Axin Protein: A scaffolding protein that is a critical component of the axin signaling complex which binds to ADENOMATOUS POLYPOSIS COLI PROTEIN; GLYCOGEN SYNTHASE KINASE 3; and CASEIN KINASE I.Axin Signaling Complex: A specific complex of WNT SIGNALING PATHWAY proteins that mediates the phosphorylation-dependent destruction of cytosolic BETA-CATENIN. The complex is disrupted by cell surface binding of WNT PROTEINS, which allows beta-catenin levels to rise to the point where they migrate to the CELL NUCLEUS and activate transcription.Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.beta Catenin: A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.Wnt Proteins: Wnt proteins are a large family of secreted glycoproteins that play essential roles in EMBRYONIC AND FETAL DEVELOPMENT, and tissue maintenance. They bind to FRIZZLED RECEPTORS and act as PARACRINE PROTEIN FACTORS to initiate a variety of SIGNAL TRANSDUCTION PATHWAYS. The canonical Wnt signaling pathway stabilizes the transcriptional coactivator BETA CATENIN.Adenomatous Polyposis Coli Protein: A negative regulator of beta-catenin signaling which is mutant in ADENOMATOUS POLYPOSIS COLI and GARDNER SYNDROME.Wnt Signaling Pathway: A complex signaling pathway whose name is derived from the DROSOPHILA Wg gene, which when mutated results in the wingless phenotype, and the vertebrate INT gene, which is located near integration sites of MOUSE MAMMARY TUMOR VIRUS. The signaling pathway is initiated by the binding of WNT PROTEINS to cells surface WNT RECEPTORS which interact with the AXIN SIGNALING COMPLEX and an array of second messengers that influence the actions of BETA CATENIN.Smad7 Protein: An inhibitory smad protein that associates with TRANSFORMING GROWTH FACTOR BETA RECEPTORS and BONE MORPHOGENETIC PROTEIN RECEPTORS. It negatively regulates SIGNAL TRANSDUCTION PATHWAYS by inhibiting PHOSPHORYLATION of RECEPTOR-REGULATED SMAD PROTEINS.Wnt3A Protein: A Wnt protein subtype that plays a role in cell-cell signaling during EMBRYONIC DEVELOPMENT and the morphogenesis of the developing NEURAL TUBE.Wnt3 Protein: A Wnt protein subtype that plays a role in cell-cell signaling during EMBRYONIC DEVELOPMENT and the morphogenesis of the developing NEURAL TUBE. Defects in Wnt3 protein are associated with autosomal recessive tetra-AMELIA in humans.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Peer Group: Group composed of associates of same species, approximately the same age, and usually of similar rank or social status.Video Games: A form of interactive entertainment in which the player controls electronically generated images that appear on a video display screen. This includes video games played in the home on special machines or home computers, and those played in arcades.Videotape Recording: Recording of visual and sometimes sound signals on magnetic tape.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Peer Review, Research: The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.Peer Review: An organized procedure carried out by a select committee of professionals in evaluating the performance of other professionals in meeting the standards of their specialty. Review by peers is used by editors in the evaluation of articles and other papers submitted for publication. Peer review is used also in the evaluation of grant applications. It is applied also in evaluating the quality of health care provided to patients.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Genome, Human: The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).TennesseeMoon: The natural satellite of the planet Earth. It includes the lunar cycles or phases, the lunar month, lunar landscapes, geography, and soil.Faculty, Medical: The teaching staff and members of the administrative staff having academic rank in a medical school.Developmental Biology: The field of biology which deals with the process of the growth and differentiation of an organism.Placozoa: A phylum of primitive invertebrate animals that exemplify a simple body organization. Trichoplax adhaerens is considered a key species for early metazoan evolution.Beak: In some animals, the jaws together with their horny covering. The beak usually refers to the bill of birds in which the whole varies greatly in form according of the food and habits of the bird. While the beak refers most commonly to birds, the anatomical counterpart is found also in the turtle, squid, and octopus. (From Webster, 3d ed & Storer, et al., General Zoology, 6th ed, p491, 755)Neural Crest: The two longitudinal ridges along the PRIMITIVE STREAK appearing near the end of GASTRULATION during development of nervous system (NEURULATION). The ridges are formed by folding of NEURAL PLATE. Between the ridges is a neural groove which deepens as the fold become elevated. When the folds meet at midline, the groove becomes a closed tube, the NEURAL TUBE.TCF Transcription Factors: A family of DNA-binding proteins that are primarily expressed in T-LYMPHOCYTES. They interact with BETA CATENIN and serve as transcriptional activators and repressors in a variety of developmental processes.Liver Regeneration: Repair or renewal of hepatic tissue.ArchivesHepatectomy: Excision of all or part of the liver. (Dorland, 28th ed)Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Adenocarcinoma, Bronchiolo-Alveolar: A carcinoma thought to be derived from epithelium of terminal bronchioles, in which the neoplastic tissue extends along the alveolar walls and grows in small masses within the alveoli. Involvement may be uniformly diffuse and massive, or nodular, or lobular. The neoplastic cells are cuboidal or columnar and form papillary structures. Mucin may be demonstrated in some of the cells and in the material in the alveoli, which also includes denuded cells. Metastases in regional lymph nodes, and in even more distant sites, are known to occur, but are infrequent. (From Stedman, 25th ed)Adenomatosis, Pulmonary: A neoplastic disease in which the alveoli and distal bronchi are filled with mucus and mucus-secreting columnar epithelial cells. It is characterized by abundant, extremely tenacious sputum, chills, fever, cough, dyspnea, and pleuritic pain. (Stedman, 25th ed)Lung Neoplasms: Tumors or cancer of the LUNG.Hyperplasia: An increase in the number of cells in a tissue or organ without tumor formation. It differs from HYPERTROPHY, which is an increase in bulk without an increase in the number of cells.Glyceraldehyde-3-Phosphate Dehydrogenases: Enzymes that catalyze the dehydrogenation of GLYCERALDEHYDE 3-PHOSPHATE. Several types of glyceraldehyde-3-phosphate-dehydrogenase exist including phosphorylating and non-phosphorylating varieties and ones that transfer hydrogen to NADP and ones that transfer hydrogen to NAD.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Precancerous Conditions: Pathological processes that tend eventually to become malignant. (From Dorland, 27th ed)

Axin prevents Wnt-3a-induced accumulation of beta-catenin. (1/400)

When Axin, a negative regulator of the Wnt signaling pathway, was expressed in COS cells, it coeluted with glycogen synthase kinase-3beta (GSK-3beta), beta-catenin, and adenomatous polyposis coli protein (APC) in a high molecular weight fraction on gel filtration column chromatography. In this fraction, GSK-3beta, beta-catenin, and APC were co-precipitated with Axin. Although beta-catenin was detected in the high molecular weight fraction in L cells on gel filtration column chromatography, addition of conditioned medium expressing Wnt-3a to the cells increased beta-catenin in the low molecular weight fraction. However, Wnt-3a-dependent accumulation of beta-catenin was greatly inhibited in L cells stably expressing Axin. Axin also suppressed Wnt-3a-dependent activation of Tcf-4 which binds to beta-catenin and acts as a transcription factor. These results suggest that Axin forms a complex with GSK-3beta, beta-catenin, and APC, resulting in the stimulation of the degradation of beta-catenin and that Wnt-3a induces the dissociation of beta-catenin from the Axin complex and accumulates beta-catenin.  (+info)

Xenopus axin interacts with glycogen synthase kinase-3 beta and is expressed in the anterior midbrain. (2/400)

Axin is encoded by the fused locus in mice and is required for normal vertebrate axis formation. It has recently been shown that axin associates with APC, beta-catenin and glycogen synthase kinase-3 (GSK-3) in a complex that appears to regulate the level of cytoplasmic beta-catenin. We have identified the Xenopus homologue of axin through its interaction with GSK-3b. Xenopus axin (Xaxin) is expressed maternally and throughout early development with a low level of ubiquitous expression. Xaxin also shows remarkably high expression in the anterior mesencephalon adjacent to the forebrain-midbrain boundary.  (+info)

Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin. (3/400)

Wnt/Wingless directs many cell fates during development. Wnt/Wingless signaling increases the amount of beta-catenin/Armadillo, which in turn activates gene transcription. Here the Drosophila protein D-Axin was shown to interact with Armadillo and D-APC. Mutation of d-axin resulted in the accumulation of cytoplasmic Armadillo and one of the Wingless target gene products, Distal-less. Ectopic expression of d-axin inhibited Wingless signaling. Hence, D-Axin negatively regulates Wingless signaling by down-regulating the level of Armadillo. These results establish the importance of the Axin family of proteins in Wnt/Wingless signaling in Drosophila.  (+info)

Phosphorylation of axin, a Wnt signal negative regulator, by glycogen synthase kinase-3beta regulates its stability. (4/400)

Axin forms a complex with glycogen synthase kinase-3beta (GSK-3beta) and beta-catenin and promotes GSK-3beta-dependent phosphorylation of beta-catenin, thereby stimulating the degradation of beta-catenin. Because GSK-3beta also phosphorylates Axin in the complex, the physiological significance of the phosphorylation of Axin was examined. Treatment of COS cells with LiCl, a GSK-3beta inhibitor, and okadaic acid, a protein phosphatase inhibitor, decreased and increased, respectively, the cellular protein level of Axin. Pulse-chase analyses showed that the phosphorylated form of Axin was more stable than the unphosphorylated form and that an Axin mutant, in which the possible phosphorylation sites for GSK-3beta were mutated, exhibited a shorter half-life than wild type Axin. Dvl-1, which was genetically shown to function upstream of GSK-3beta, inhibited the phosphorylation of Axin by GSK-3beta in vitro. Furthermore, Wnt-3a-containing conditioned medium down-regulated Axin and accumulated beta-catenin in L cells and expression of Dvl-1(DeltaPDZ), in which the PDZ domain was deleted, suppressed this action of Wnt-3a. These results suggest that the phosphorylation of Axin is important for the regulation of its stability and that Wnt down-regulates Axin through Dvl.  (+info)

An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of beta-catenin. (5/400)

beta-catenin plays an essential role in the Wingless/Wnt signaling cascade and is a component of the cadherin cell adhesion complex. Deregulation of beta-catenin accumulation as a result of mutations in adenomatous polyposis coli (APC) tumor suppressor protein is believed to initiate colorectal neoplasia. beta-catenin levels are regulated by the ubiquitin-dependent proteolysis system and beta-catenin ubiquitination is preceded by phosphorylation of its N-terminal region by the glycogen synthase kinase-3beta (GSK-3beta)/Axin kinase complex. Here we show that FWD1 (the mouse homologue of Slimb/betaTrCP), an F-box/WD40-repeat protein, specifically formed a multi-molecular complex with beta-catenin, Axin, GSK-3beta and APC. Mutations at the signal-induced phosphorylation site of beta-catenin inhibited its association with FWD1. FWD1 facilitated ubiquitination and promoted degradation of beta-catenin, resulting in reduced cytoplasmic beta-catenin levels. In contrast, a dominant-negative mutant form of FWD1 inhibited the ubiquitination process and stabilized beta-catenin. These results suggest that the Skp1/Cullin/F-box protein FWD1 (SCFFWD1)-ubiquitin ligase complex is involved in beta-catenin ubiquitination and that FWD1 serves as an intracellular receptor for phosphorylated beta-catenin. FWD1 also links the phosphorylation machinery to the ubiquitin-proteasome pathway to ensure prompt and efficient proteolysis of beta-catenin in response to external signals. SCFFWD1 may be critical for tumor development and suppression through regulation of beta-catenin protein stability.  (+info)

Functional domains of axin. Importance of the C terminus as an oligomerization domain. (6/400)

To understand the mechanism of how Axin acts as an inhibitory molecule in the Wnt pathway, we generated a series of mutated forms of Axin. From the binding experiments, we defined the domains of Axin that bind glycogen synthase kinase-3beta (GSK-3beta) and beta-catenin. We also examined the ability of each Axin mutant to inhibit lymphoid enhancer factor-1 (Lef-1) reporter activity in a cell line expressing high levels of beta-catenin. Axin mutants that did not bind GSK-3beta or beta-catenin were ineffective in suppressing Lef-1 reporter activity. Binding GSK-3beta and beta-catenin was not sufficient for this inhibitory effect of Axin. Axin mutants with C-terminal truncations lacked the ability to inhibit Lef-1 reporter activity, even though they bound GSK-3beta and beta-catenin. The C-terminal region was required for binding to Axin itself. Substitution of the C-terminal region with an unrelated dimerizing molecule, the retinoid X receptor restored its inhibitory effect on Lef-1-dependent transcription. The oligomerization of Axin through its C terminus is important for its function in regulation of beta-catenin-mediated response.  (+info)

The cyclin D1 gene is a target of the beta-catenin/LEF-1 pathway. (7/400)

beta-Catenin plays a dual role in the cell: one in linking the cytoplasmic side of cadherin-mediated cell-cell contacts to the actin cytoskeleton and an additional role in signaling that involves transactivation in complex with transcription factors of the lymphoid enhancing factor (LEF-1) family. Elevated beta-catenin levels in colorectal cancer caused by mutations in beta-catenin or by the adenomatous polyposis coli molecule, which regulates beta-catenin degradation, result in the binding of beta-catenin to LEF-1 and increased transcriptional activation of mostly unknown target genes. Here, we show that the cyclin D1 gene is a direct target for transactivation by the beta-catenin/LEF-1 pathway through a LEF-1 binding site in the cyclin D1 promoter. Inhibitors of beta-catenin activation, wild-type adenomatous polyposis coli, axin, and the cytoplasmic tail of cadherin suppressed cyclin D1 promoter activity in colon cancer cells. Cyclin D1 protein levels were induced by beta-catenin overexpression and reduced in cells overexpressing the cadherin cytoplasmic domain. Increased beta-catenin levels may thus promote neoplastic conversion by triggering cyclin D1 gene expression and, consequently, uncontrolled progression into the cell cycle.  (+info)

Interaction of axin and Dvl-2 proteins regulates Dvl-2-stimulated TCF-dependent transcription. (8/400)

Axin promotes the phosphorylation of beta-catenin by GSK-3beta, leading to beta-catenin degradation. Wnt signals interfere with beta-catenin turnover, resulting in enhanced transcription of target genes through the increased formation of beta-catenin complexes containing TCF transcription factors. Little is known about how GSK-3beta-mediated beta-catenin turnover is regulated in response to Wnt signals. We have explored the relationship between Axin and Dvl-2, a member of the Dishevelled family of proteins that function upstream of GSK-3beta. Expression of Dvl-2 activated TCF-dependent transcription. This was blocked by co-expression of GSK-3beta or Axin. Expression of a 59 amino acid GSK-3beta-binding region from Axin strongly activated transcription in the absence of an upstream signal. Introduction of a point mutation into full-length Axin that prevented GSK-3beta binding also generated a transcriptional activator. When co-expressed, Axin and Dvl-2 co-localized within expressing cells. When Dvl-2 localization was altered using a C-terminal CAAX motif, Axin was also redistributed, suggesting a close association between the two proteins, a conclusion supported by co-immunoprecipitation data. Deletion analysis suggested that Dvl-association determinants within Axin were contained between residues 603 and 810. The association of Axin with Dvl-2 may be important in the transmission of Wnt signals from Dvl-2 to GSK-3beta.  (+info)

*SENP2

... a novel Axin-binding protein, is involved in downregulation of beta-catenin". Mol. Cell. Biol. 22 (11): 3803-19. doi:10.1128/ ... SUMO1 (UBL1; MIM 601912) is a small ubiquitin-like protein that can be covalently conjugated to other proteins. SENP2 is one of ... which is an axin-binding protein promoting beta-catenin degradation". J. Biol. Chem. 276 (42): 39060-6. doi:10.1074/jbc. ... The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (1): 65-73. doi: ...

*SUMO3

... a novel Axin-binding protein, is involved in downregulation of beta-catenin". Molecular and Cellular Biology. 22 (11): 3803-19 ... Small ubiquitin-related modifier 3 is a protein that in humans is encoded by the SUMO3 gene. SUMO proteins, such as SUMO3, and ... Su HL, Li SS (August 2002). "Molecular features of human ubiquitin-like SUMO genes and their encoded proteins". Gene. 296 (1-2 ... Eaton EM, Sealy L (August 2003). "Modification of CCAAT/enhancer-binding protein-beta by the small ubiquitin-like modifier ( ...

*Beta-catenin

The scaffold protein axin (two closely related paralogs, axin 1 and axin 2) contains a similar interaction motif on its long, ... This way, axin acts as a true scaffold protein, bringing an enzyme (GSK3) together with its substrate (β-catenin) into close ... But even axin does not act alone. Through its N-terminal regulator of G-protein signaling (RGS) domain, it recruits the ... Beta-catenin contains no such motifs, but a special protein does: axin. What is more, its GSK3 docking motif is directly ...

*ZBED3

Zinc finger BED domain-containing protein 3 also known as axin-interacting protein is a protein in humans that is encoded by ... as a novel Axin-interacting protein that activates Wnt/beta-catenin signaling". The Journal of Biological Chemistry. 284 (11): ...

*DYNLT1

Strovel ET, Wu D, Sussman DJ (2000). "Protein phosphatase 2Calpha dephosphorylates axin and activates LEF-1-dependent ... Dynein light chain Tctex-type 1 is a protein that in humans is encoded by the DYNLT1 gene. Cytoplasmic dynein is the major ... 2004). "Herpes simplex virus type 1 capsid protein VP26 interacts with dynein light chains RP3 and Tctex1 and plays a role in ... 1997). "The mouse t-complex-encoded protein Tctex-1 is a light chain of brain cytoplasmic dynein". J. Biol. Chem. 271 (50): ...

*DVL2

2004). "The DIX domain protein coiled-coil-DIX1 inhibits c-Jun N-terminal kinase activation by Axin and dishevelled through ... Segment polarity protein dishevelled homolog DVL-2 is a protein that in humans is encoded by the DVL2 gene. This gene encodes a ... Strovel ET, Wu D, Sussman DJ (2000). "Protein phosphatase 2Calpha dephosphorylates axin and activates LEF-1-dependent ... 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. ...

*AXIN1

Axin-1 is a protein that in humans is encoded by the AXIN1 gene. This gene encodes a cytoplasmic protein which contains a ... Fagotto F, Jho Eh, Zeng L, Kurth T, Joos T, Kaufmann C, Costantini F (1999). "Domains of axin involved in protein-protein ... cadherin-associated protein) beta 1, glycogen synthase kinase 3 beta, protein phosphatase 2, and itself. This protein functions ... Hsu W, Zeng L, Costantini F (1999). "Identification of a domain of Axin that binds to the serine/threonine protein phosphatase ...

*AXIN2

Axin-2 also known as axin-like protein (Axil) or axis inhibition protein 2 (AXIN2) or conductin is a protein that in humans is ... The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the ... "Axin is a scaffold protein in TGF-β signaling that promotes degradation of Smad7 by Arkadia". EMBO J. 25 (8): 1646-58. doi: ... "Entrez Gene: AXIN2 axin 2 (conductin, axil)". Vastardis H (June 2000). "The genetics of human tooth agenesis: new discoveries ...

*RNF111

"Axin is a scaffold protein in TGF-beta signaling that promotes degradation of Smad7 by Arkadia". The EMBO Journal. 25 (8): 1646 ... The protein encoded by this gene contains a RING finger domain, a motif known to be involved in protein-protein and protein-DNA ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038/ ... "Entrez Gene: RNF111 ring finger protein 111". Koinuma D, Shinozaki M, Komuro A, Goto K, Saitoh M, Hanyu A, Ebina M, Nukiwa T, ...

*GSK3B

Kishida S, Yamamoto H, Hino S, Ikeda S, Kishida M, Kikuchi A (Jun 1999). "DIX domains of Dvl and axin are necessary for protein ... Guo X, Ramirez A, Waddell DS, Li Z, Liu X, Wang XF (Jan 2008). "Axin and GSK3- control Smad3 protein stability and modulate TGF ... Nakamura T, Hamada F, Ishidate T, Anai K, Kawahara K, Toyoshima K, Akiyama T (Jun 1998). "Axin, an inhibitor of the Wnt ... Tanji C, Yamamoto H, Yorioka N, Kohno N, Kikuchi K, Kikuchi A (Oct 2002). "A-kinase anchoring protein AKAP220 binds to glycogen ...

*Ubiquitin C

Guo X, Ramirez A, Waddell DS, Li Z, Liu X, Wang XF (January 2008). "Axin and GSK3- control Smad3 protein stability and modulate ... "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi:10.1016/j.cell ... Polyubiquitin-C is a protein encoded by the UBC gene in humans. Polyubiquitin-C is one of the sources of ubiquitin, along with ... Chen L, Dong W, Zou T, Ouyang L, He G, Liu Y, Qi Y (August 2008). "Protein phosphatase 4 negatively regulates LPS cascade by ...

*Wnt signaling pathway

Phosphorylation by other proteins in the destruction complex subsequently binds Axin to the cytoplasmic tail of LRP5/6. Axin ... This destruction complex includes the following proteins: Axin, adenomatosis polyposis coli (APC), protein phosphatase 2A (PP2A ... Dsh proteins are present in all organisms and they all share the following highly conserved protein domains: an amino-terminal ... For example, Wnt proteins are palmitoylated. The protein porcupine mediates this process, which means that it helps regulate ...

*DVL1

Fagotto F, Jho Eh, Zeng L, Kurth T, Joos T, Kaufmann C, Costantini F (1999). "Domains of axin involved in protein-protein ... Kishida S, Yamamoto H, Hino S, Ikeda S, Kishida M, Kikuchi A (Jun 1999). "DIX domains of Dvl and axin are necessary for protein ... Kishida S, Yamamoto H, Hino S, Ikeda S, Kishida M, Kikuchi A (1999). "DIX domains of Dvl and axin are necessary for protein ... Segment polarity protein dishevelled homolog DVL-1 is a protein that in humans is encoded by the DVL1 gene. DVL1, the human ...

*LRP5

"Low-density lipoprotein receptor-related protein-5 binds to Axin and regulates the canonical Wnt signaling pathway". Molecular ... Low-density lipoprotein receptor-related protein 5 is a protein that in humans is encoded by the LRP5 gene. LRP5 is a key ... LRP5 acts as a co-receptor with LRP6 and the Frizzled protein family members for transducing signals by Wnt proteins through ... "SUMOylation target sites at the C terminus protect Axin from ubiquitination and confer protein stability". FASEB Journal. 22 ( ...

*DVL3

1999). "DIX domains of Dvl and axin are necessary for protein interactions and their ability to regulate beta-catenin stability ... Segment polarity protein dishevelled homolog DVL-3 is a protein that in humans is encoded by the DVL3 gene. This gene is a ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038 ... "DIX domains of Dvl and axin are necessary for protein interactions and their ability to regulate beta-catenin stability". Mol. ...

*WNT1

2001). "Low-density lipoprotein receptor-related protein-5 binds to Axin and regulates the canonical Wnt signaling pathway". ... Proto-oncogene protein Wnt-1 is a protein that in humans is encoded by the WNT1 gene. The WNT gene family consists of ... It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein ... These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate ...

*SUMO2

... which is an axin-binding protein promoting beta-catenin degradation". The Journal of Biological Chemistry. 276 (42): 39060-6. ... Small ubiquitin-related modifier 2 is a protein that in humans is encoded by the SUMO2 gene. This gene encodes a protein that ... However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, ... "In vitro modification of human centromere protein CENP-C fragments by small ubiquitin-like modifier (SUMO) protein: definitive ...

*GSK-3

... while the phosphorylation of Beta-catenin by GSK-3 is mediated by the binding of both proteins to Axin, a scaffold protein, ... GSK-3 is thought to directly promote Aβ production and to be tied to the process of the hyperphosphorylation of tau proteins, ... Woodgett JR (September 2001). "Judging a protein by more than its name: GSK-3". Sci. STKE. 2001 (100): RE12. doi:10.1126/stke. ... Glycogen synthase kinase 3 is a serine/threonine protein kinase that mediates the addition of phosphate molecules onto serine ...

*PPP2R5B

... of adenomatous polyposis coli gene product can be modulated by beta-catenin and protein phosphatase 2A complexed with Axin". ... "Direct activation of protein phosphatase-2A0 by HIV-1 encoded protein complex NCp7:vpr". FEBS Letters. 401 (2-3): 197-201. doi: ... Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell ... Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform is an enzyme that in humans is encoded by the ...

*PPP2R5C

... of adenomatous polyposis coli gene product can be modulated by beta-catenin and protein phosphatase 2A complexed with Axin". ... The product of this gene belongs to the Protein phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of ... "Direct activation of protein phosphatase-2A0 by HIV-1 encoded protein complex NCp7:vpr". FEBS Letters. 401 (2-3): 197-201. doi: ... Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform is an enzyme that in humans is encoded by the ...

*Dishevelled

... of dishevelled in Drosophila defines novel domains in the dishevelled protein as well as novel suppressing alleles of axin". ... There are three human genes that encode for the dishevelled proteins: DVL1 DVL2 DVL3 Penton A, Wodarz A, Nusse R (June 2002). " ... Dishevelled (Dsh) is a family of proteins involved in canonical and non-canonical Wnt signalling pathways. Dsh is a cytoplasmic ... dishevelled proteins at the US National Library of Medicine Medical Subject Headings (MeSH). ...

*PPP2CB

Hsu W, Zeng L, Costantini F (Feb 1999). "Identification of a domain of Axin that binds to the serine/threonine protein ... "Direct activation of protein phosphatase-2A0 by HIV-1 encoded protein complex NCp7:vpr". FEBS Letters. 401 (2-3): 197-201. doi: ... is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A". The Journal of ... "p42 MAP kinase phosphorylation sites in microtubule-associated protein tau are dephosphorylated by protein phosphatase 2A1. ...

*Secreted frizzled-related protein 1

Axin is a scaffolding protein which holds the degradation complex together. The activated Dvl associates with Axin and prevents ... the SFRP1 netrin-related motif is also found in a range of other proteins that is thought to mediate protein-protein ... Secreted frizzled-related protein 1, also known as SFRP1, is a protein which in humans is encoded by the SFRP1 gene. Secreted ... SFRP1 are heparin-binding proteins, with the heparin-binding domain within the C-terminal region of the SFRP1 protein. In vitro ...

*Autocrine signalling

... leads to stabilization of β-catenin through inactivation of a protein complex containing the tumor suppressors APC and Axin. ... De-regulation of the autocrine Wnt signaling pathway via mutations in APC and Axin have been linked to activation of various ... In colorectal cancer, for example, mutations in APC, axin, or β-catenin promote β-catenin stabilization and transcription of ... genes encoding cancer-associated proteins. Furthermore, in human breast cancer, interference with the de-regulated Wnt ...

*DIXDC1

"The DIX domain protein coiled-coil-DIX1 inhibits c-Jun N-terminal kinase activation by Axin and dishevelled through distinct ... Dixin is a protein that in humans is encoded by the DIXDC1 gene. When active it stops cancer metastasis due to extreme ... The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 7 (6): 347-55. ... "KIAA1735 gene on human chromosome 11q23.1 encodes a novel protein with myosine-tail homologous domain and C-terminal DIX domain ...

*Catenin

... s are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two ... APC or Axin, each of which can lead to this de-regulated β-catenin level stabilization in cells. While less attention is ... Mutations in genes encoding these proteins can lead to inactivation of cadherin cell adhesions and elimination of contact ... "Adjuvant immunochemotherapy with protein-bound polysaccharide K for colon cancer in relation to oncogenic β-catenin activation ...
Colorectal cancer is the second leading cause of cancer incidence and cancer death among adults in Europe. It is estimated for the year 2000 alone that 362,620 people were diagnosed with colorectal cancer, and 198,778 patients died owing to this disease (Ferlay et al., 2001). In more than 80% of the sporadic colorectal cancers, both alleles of the Adenomatous Polyposis Coli (APC) gene are inactivated (Kinzler and Vogelstein, 1996). The APC protein forms - together with Axin and GSK3β - a degradation complex for β-catenin. In this complex, GSK3β phosphorylates β-catenin, which in turn is ubiquitinated and thereby targeted for destruction. Wnt signalling inhibits the degradation complex and hence leads to the cytoplasmic accumulation and entry of β-catenin into the nucleus, where it forms a complex with members of the Pangolin(Pan)/TCF/Lef family of DNA-binding proteins, the putative adaptor protein Legless/BCL9 (Lgs) and the transcriptional regulator Pygopus (Pygo) (Behrens et al., 1996; ...
This is, to our knowledge, the first report describing active canonical Wnt together with YAP/Taz/TEAD signaling in adult skeletal muscle fibers. Using Axin2-lacZ reporter mice, we identified active canonical Wnt signaling in type II myofibers (Fig. 2) (Lustig et al., 2002). This mouse model is well established for tracing active canonical Wnt signaling since Axin2 is a direct target of β-catenin-mediated gene expression (Barolo, 2006). In fact, heterozygous Axin2-lacZ mice, without any known signs of haploinsufficiency, express muscular β-gal in type II muscle fibers, suggesting that active canonical Wnt signaling is present physiologically in adult muscle fibers (Fig. 2). In homozygous Axin2-lacZ mice, not only is the doubled lacZ gene dosage responsible for elevated Axin2-lacZ reporter expression, but also the derepression of canonical Wnt signaling, since Axin2 itself is a negative regulator and target of canonical Wnt signaling and therefore participates in a negative-feedback loop. In ...
Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (By similarity). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity). Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling (By similarity ...
In cells not exposed to the signal, β-catenin levels are kept low through interactions with the protein kinase zw3/GSK-3, CK1a, APC and Axin (Behrens, 1998 Itoh 1998., Hamada, 1999.) β-catenin is degraded, after phosphorylation by GSK-3 and CK1 alpha (Yanagawa 2002, Liu 2002, Amit 2002), through the ubiquitin pathway (Aberle 1997.), involving interactions with Slimb/bTrCP (Jiang 1998, Marikawa 1998,; reviewed in Maniatis 1999). In a current model, Wnt signaling initially leads to a complex between Dsh, GBP/Frat1, Axin and Zw3/GSK, which may be the regulatory step in the inactivation of Zw3/GSK (Salic, 2000; Farr 2000). The DIX domain in Axin is similar to the NH2 terminus in Dsh, and promotes interactions between Dsh and Axin (Hsu 1999, Smalley, 1999). As a consequence, GSK does not phosphorylate β-catenin anymore, releasing it from the Axin complex and accumulation (Salic, 2000).The stabilized β-catenin then enters the nucleus to interact with TCF. β-catenin can convert TCF into a ...
There are two genes that encode apc in Drosophila, apc 1 and 2. They are largely redundant, and only double mutants lead to strong pathway activation in embryos (Ahmed et al. 2002; McCartney et al. 2006). In apc1 apc2 double mutants, I assayed the ability of membrane-tethered apc2 to block signaling activation. As shown in Figure 1H, there was no real effect on the patterning of the cuticle. In contrast, expression of untethered apc2 rescued to a wild-type cuticle pattern (Figure 1G). I further used the apc1 apc2 double mutants to test the activity of both membrane-tethered and -untethered axin. Interestingly, expression of axin abolished signaling brought about by loss of both apc genes leading to a completely denticle-covered cuticle (Figure 1E). The tethered axin, however, was unable to overcome the loss of apc and signaling was not blocked (naked phenotype, Figure 1F).. To further assay the activity of the membrane-tethered and -untethered apc2 and axin, I expressed all four in a triple ...
Four candidate genes were differentially expressed in PCa recurrence. Of these, low AXIN2 expression was internally validated in the discovery cohort. Validation in external cohorts and in silico demonstrated that low AXIN2 was independently associated with more aggressive PCa, biochemical recurrence, and metastasis-free survival after RP. Functionally, siRNA-mediated depletion of AXIN2 significantly increased invasiveness, proliferation, and tumor growth. Conversely, ectopic overexpression of AXIN2 significantly reduced invasiveness, proliferation, and tumor growth ...
Complete information for AXIN2 gene (Protein Coding), Axin 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Negative regulator of the canonical Wnt signaling pathway involved in neuroectodermal patterning. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex ...
Gentaur molecular products has all kinds of products like :search , Prospecbio \ Mouse Anti Human Axin-1 AXIN1 \ ant-021 for more molecular products just contact us
APC and AXIN2 are important tumor suppressors that function in the Wnt signaling pathway by regulating the level of β-catenin. APC and AXIN2 are essential interacting components of the β-catenin destruction complex that function to down-regulate β-catenin through feedback inhibition (32, 33). Although germ line and somatic APC mutations are infrequent in human primary breast tumors (34, 35), the predisposition of the ApcMin mouse model provides strong evidence in support of a role for APC in breast cancer (18). Specifically, the Apc-deficient murine mammary glands develop metaplasia and rapidly progress to neoplasia in the presence of p53 mutations (36). Similarly, Axin2 knockout mice are more prone to carcinogen-induced neoplasia. In addition, epithelial-to-mesenchymal transition in invasive human breast cancers is associated with an AXIN2-dependent pathway that stabilizes β-catenin and GSK3β and regulates SNAIL1 activity (37). These observations led to the hypothesis that genetic ...
Compare & find the top performing anti-Mouse (Murine) AXIN1 antibody for Immunofluorescence (Paraffin-embedded Sections) (IF (p)).
Human AXIN2 partial ORF ( NP_004646, 611 a.a. - 710 a.a.) recombinant protein with GST-tag at N-terminal. (H00008313-Q02) - Products - Abnova
Axin 2 antibody (axin 2) for ICC/IF, IHC-P, WB. Anti-Axin 2 pAb (GTX30080) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
The Wnt family of growth factors and components of their signalling pathways have diverse roles in development and disease. Wnt signalling influences many aspects of embryonic patterning, cell proliferation as well as the maintenance and differentiation of stem cells, and is critical in axis formation (figure 1b; reviewed in Moon et al. 2004; Kohn & Moon 2005; Clevers 2006). In the absence of the so-called canonical Wnt signalling (Wnt/β-catenin), β-catenin is rapidly sequestered in a cytoplasmic degradation complex containing axin, the adenomatous polyposis tumour suppressor protein (APC) and the serine threonine kinase GSK-3β. GSK-3 phosphorylation of β-catenin targets the latter for proteasomal degradation (figure 1b). Wnt binding to its co-receptors Frizzled and LRP5/6 (low-density lipoprotein receptor-related protein) activates a cytoplasmic phosphoprotein (Dishevelled, Dsh) which downregulates GSK-3 and inhibits the degradation of β-catenin. Stabilized β-catenin protein interacts ...
Wnt proteins are secreted morphogens that are required for basic developmental processes, such as cell-fate specification, progenitor-cell proliferation and the control of asymmetric cell division, in many different species and organs. There are at least three different Wnt pathways: the canonical pathway, the planar cell polarity (PCP) pathway and the Wnt/Ca2+ pathway. In the canonical Wnt pathway, the major effect of Wnt ligand binding to its receptor is the stabilization of cytoplasmic beta-catenin through inhibition of the bea-catenin degradation complex. Beta-catenin is then free to enter the nucleus and activate Wnt-regulated genes through its interaction with TCF (T-cell factor) family transcription factors and concomitant recruitment of coactivators. Planar cell polarity (PCP) signaling leads to the activation of the small GTPases RHOA (RAS homologue gene-family member A) and RAC1, which activate the stress kinase JNK (Jun N-terminal kinase) and ROCK (RHO-associated ...
Next, α catenin links bound β catenin and γ catenin to the actin filaments of the cell cytoskeleton via interactions with various actin binding proteins or by binding actin itself.24-26 The p120 protein, however, binds at a unique site directly to E-cadherin that is bound to either β catenin or γ catenin without linking it to the actin cytoskeleton.24-26 The p120 protein has been reported to act as an inhibitor of cadherin mediated adhesion, although the actual mechanism of inhibition is unclear.27. The β catenin molecule interacts with the adenomatous polyposis coli (APC) tumour suppressor protein, glycogen synthase kinase β, and an adaptor protein axin (or a homologue, conductin) in a complex, which is responsible for its degradation.28 This destruction complex is essential to maintain appropriate concentrations of free cytoplasmic β catenin. APC and E-cadherin have been shown to compete for the same binding region on β catenin.29 It has been suggested that APC modulates the ...
Aqeilan, R. I., et al. (2005). WW domain-containing proteins, WWOX and YAP, compete for interaction with ErbB-4 and modulate its transcriptional function. Cancer Res. 65(15): 6764-72. 16061658 Alarcon, C., et al.. (2009). Nuclear CDKs drive Smad transcriptional activation and turnover in BMP and TGF-beta pathways. Cell 139(4): 757-69. PubMed Citation: 19914168 Aragón, E., et al. (2011). A Smad action turnover switch operated by WW domain readers of a phosphoserine code. Genes Dev. 25(12): 1275-88. PubMed Citation: 21685363 Azzolin, L., Panciera, T., Soligo, S., Enzo, E., Bicciato, S., Dupont, S., Bresolin, S., Frasson, C., Basso, G., Guzzardo, V., Fassina, A., Cordenonsi, M. and Piccolo, S. (2014). YAP/TAZ incorporation in the beta-Catenin destruction complex orchestrates the Wnt response. Cell 158: 157-170. PubMed ID: 24976009 Badouel, C., et al. (2009). The FERM-domain protein Expanded regulates Hippo pathway activity via direct interactions with the transcriptional activator Yorkie. Dev. ...
At the base of the glands, however, there are long lived stem cells, which continuously generate new cells, explains Dr. Sigal. He adds: In addition to identifying these cells, we also wanted to discover the processes which control their regeneration. Stem cell characterization revealed that there are two different types of stem cells in the stomach, both of which are positive for the protein AXIN2. We found that the cells directly below the glands produce a specific molecule known as R spondin. This molecule has a major effect on stem cells. It drives cell division in one type of stem cell, thereby increasing the speed of gastric gland regeneration, says the researcher. Infection with H. pylori results in an increased production of R spondin and increased stem cell activity. It is likely that a sustained high rate of stem cell proliferation promotes cancer formation ...
Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation, By similarity. {ECO:0000250, ECO:0000269,PubMed:11357136, ECO:0000269,PubMed:11448771, ECO:0000269,PubMed:15778503, ECO:0000269,PubMed:16341017, ECO:0000269,PubMed:16513652, ECO:0000269,PubMed:17326769, ECO:0000269,PubMed:17400545, ECO:0000269,PubMed:19107203, ECO:0000269,PubMed:19293931, ECO:0000269,PubMed:19801552 ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Rabbit polyclonal Axin 2 antibody validated for WB, IHC, ICC/IF and tested in Human, Mouse and Rat. With 1 independent review. Immunogen corresponding to…
Bonjour, je rcherche la procedure pour flasher un axil ad-600 new usual et les programmes necessaires. En cherchant sur le forum jai eu de bonnes pistes mais souvent les liens sont mort.|-(
Qtº + pesquiso + dúvidas me surgem. Será q alguém me pode indicar qual o firm correcto para este receptor e como se colocam a k *** no mesmo?
beta-catenin destruction complex, beta-catenin-TCF7L2 complex, catenin complex, cell cortex, cell junction, cell periphery, cell-cell adherens junction, cell-cell junction, centrosome, cytoplasm
Rabbit polyclonal antibody raised against synthetic peptide of Lytechinus variegatus AXIN. A synthetic peptide corresponding to C-terminus of Lytechinus variegatus AXIN. (PAB16080) - Products - Abnova
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
0 ➲ downloads ▶▶ AXIL™ Logo vector ◀◀ Download AXIL Vector Logo for Free. AXIL vector logo in EPS vector format for Adobe Illustrator, Corel Draw and others vector editors (Win/Mac/Linux)
Plasmid pAG228 TNKS2 3UTR mut from Dr. David Bartels lab contains the insert TNKS2 3UTR mut and is published in Science. 2005 Dec 16. 310(5755):1817-21. This plasmid is available through Addgene.
Axil RT 202 - Digital Receiver mit Testberichten und Angeboten ab € (Stand ) im Preisvergleich bei dooyoo.de - Kaufberatung und Tests f r Axil - Digital Receiver
The AXIL Z Flush Mount Power and Data Center is made of an anodized aluminum frame that can be installed on any desk or table top incorporates waterproof outlets engineered for maximum protection against liquid spills preventing electric shocks.
Information about Dix Hills NY public health major. You can enter healthcare with either an associates degree or a bachelors. As a medical professional, you may provide patient care under the supervision of hospital staff.
I use these for everything. Mowing the lawn, shooting, wood work, these things are awesome. I had them waterproofed as well. Shout out to Tai for helping me. Awesome Customer service!. ...
Activity of the canonical Wnt signal pathway in kidney homogenates of sham and nephrectomized rats. The expression of active Wnt components in kidney homogenate
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Axil Swims high waisted bottom that is comfortable and flattering. Comes in Olive, Honey, Rosé, and Black. It is made of high quality shiny fabric an
Background: The Wnt signal transduction pathway is important in a wide variety of developmental processes as well as in the genesis of human cancer. Vertebrate Wnt pathways can be functionally separated into two classes ...
TNKS1BP1 Full-Length MS Protein Standard (NP_203754), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics.
|address||span style=font-size: x-small; font-family: arial,helvetica,sans-serif;||strong|Product description|/strong| |/span||/address| |ul| |li||address||span style=font-size: x-small; font-family: arial,helvetica,sans-serif;|Shorts Axil|/span||/a
Ok thanks everyone for there help, also a big cheers to the AZ crew, so awesome to be able to jump on my phone with a problem and or question an have access to all of this info and fellow zookers that have endless knowledge of these machines ...
Hiii guyzz I m back with my another epi thank u sooooo much for ur lov n support tnks to silent reader also n sorry to swasan n raglak fans for not making
Le faible écart de revenu entre le Danemark et les pays les plus avancés de l’OCDE s’est constamment creusé au cours des dix derni&#
Postsynaptic scaffolding proteins ensure efficient neurotransmission by anchoring receptors and signaling molecules in synapse-specific subcellular domains. In turn, posttranslational modifications of scaffolding proteins contribute to synaptic plasticity by remodeling the postsynaptic apparatus. Though these mechanisms are operant in glutamatergic synapses, little is known about regulation of GABAergic synapses, which mediate inhibitory transmission in the CNS. Here, we focused on gephyrin, the main scaffolding protein of GABAergic synapses. We identify a unique phosphorylation site in gephyrin, Ser270, targeted by glycogen synthase kinase 3β (GSK3β) to modulate GABAergic transmission. Abolishing Ser270 phosphorylation increased the density of gephyrin clusters and the frequency of miniature GABAergic postsynaptic currents in cultured hippocampal neurons. Enhanced, phosphorylation-dependent gephyrin clustering was also induced in vitro and in vivo with lithium chloride. Lithium is a GSK3β ...
AXIN1_HUMAN] Defects in AXIN1 are involved in hepatocellular carcinoma (HCC) [MIM:114550].[1] [2] Defects in AXIN1 are a cause of caudal duplication anomaly (CADUA) [MIM:607864]. Caudal duplication anomaly is characterized by the occurrence of duplications of different organs in the caudal region. Note=Caudal duplication anomaly is associated with hypermethylation of the AXIN1 promoter.[3] [APC_HUMAN] Defects in APC are a cause of familial adenomatous polyposis (FAP) [MIM:175100]; which includes also Gardner syndrome (GS). FAP and GS contribute to tumor development in patients with uninherited forms of colorectal cancer. FAP is characterized by adenomatous polyps of the colon and rectum, but also of upper gastrointestinal tract (ampullary, duodenal and gastric adenomas). This is a viciously premalignant disease with one or more polyps progressing through dysplasia to malignancy in untreated gene carriers with a median age at diagnosis of 40 years.[4] [5] [6] [7] [8] [9] [10] [11] [12] [13] ...
Axil Meaning in Malayalam, Axil in Malayalam, Axil Malayalam Equivalent, English to Malayalam Free Dictionary : Malayalam to English Free Dictionary : Meaning of Axil in Malayalam : Online Malayalam English Free Dictionary Online രണ്ട് ലക്ഷത്തിലധികം വാക്കുകളും അവയുടെ അർത്ഥങ്ങളും വ്യാഖ്യാനങ്ങളുമുള്ള നിഘണ്ടു ഇംഗ്ലീഷ് - മലയാളം, മലയാളം - മലയാളം നിഘണ്ടു. The biggest and fastest English-Malayalam, Malayalam-Malayalam Dictionary with hundred thousands of words and definitions
Glycogen synthase kinase 3 (GSK-3) in its active state complexes with APC and AXIN to suppress phosphorylation of β-catenin, which then gets degraded via the proteasome. GSK-3 becomes inactivated through the PI3K/AKT pathway when the cell receives signals like growth factors, cytokines, or insulin. WNT signaling can also inactivate GSK-3 through Dsh. Inactivation of GSK-3 leads to gene expression, cell survival, and cell proliferation.. Click on the poster below to view the interactive version.. ...
Axil- Keep in mind that Bose particles are those with 0 or integer intrinsic spin , either + or -. I think the theory for BEC allows for existence of a condensate of particles with different integer spin states. Kims theory for LENR addresses such a BEC I believe. The collapse of a BEC which part of an entangled system-a metal lattice for example-may allow transfer of the potential energy of the BEC to the lattice phonic (kinetic-energy) while conserving angular momentum. Bob Cook From: Axil Axil,mailto:[email protected], Sent: Monday, September 4, 2017 9:39 AM To: vortex-l,mailto:[email protected], Subject: Re: [Vo]:RE: f13C or faux13C Correction therefore decisive should read therefore destructive On Mon, Sep 4, 2017 at 12:37 PM, Axil Axil ,[email protected],mailto:[email protected],, wrote: C12 is a boson and as such is LENR capable. C13 is a fermion and therefore decisive to the formation of a bose condensate of atoms. It is reasonable to expect that C12 will aid in the production of ...
DCAT: At the organizations annual membership meeting DCAT installed Dix Weaver, Sr. supply chain consultant at Eli Lilly and company as the new president of the organization for 2008. Mr. Weaver succeeds Joe Colleluori, Lonza Inc. and is
gill dix is on ePHOTOzine, a photography site that allows photographers to share photos and ideas. gill dix uses ePHOTOzine to upload photos, comment on photos and talk about photography. Here you can view gill dixs photos. Sign Up to ePHOTOzine to communicate with gill dix, vote on their pictures and upload your own.
Depois de uma semana consegui instalar o firm e colocar a TVCabo a funcionar, mas os espanhois desapareceram... s o keys novas. Algu m para ajudar???
Technical Arboriculture Limited , 1 Chase Farm Close, Waltham Chase, Southampton, Hampshire. SO32 2UB , 01489 896655 , Reg No. 6018958 (England and Wales). ...
The (Adenomatous Polyposis Coli) APC protein normally builds a "destruction complex" with glycogen synthase kinase 3-alpha and or beta (GSK-3α/β) and axin via interactions with the 20 AA and SAMP repeats[citation needed]. This complex is then able to bind β-catenins in the cytoplasm, that have dissociated from adherens contacts between cells. With the help of casein kinase 1 (CK1), which carries out an initial phosphorylation of β-catenin, GSK-3β is able to phosphorylate β-catenin a second time. This targets β-catenin for ubiquitination and degradation by cellular proteasomes. This prevents it from translocating into the nucleus, where it acts as a transcription factor for proliferation genes. APC is also thought to be targeted to microtubules via the PDZ binding domain, stabilizing them[citation needed]. The deactivation of the APC protein can take place after certain chain reactions in the cytoplasm are started, e.g. through the Wnt signals that destroy the conformation of the ...
PTPRK‐RSPO3 rearrangements typically lead to aberrant expression of fusion transcripts by colorectal cancer cells that normally do not express RSPO3 (Sato et al, 2009; Seshagiri et al, 2012). Therefore, in principle, searching for CRC samples displaying high RSPO3 mRNA levels should pinpoint tumors bearing RSPO3 fusions. However, stromal cells have been identified as a source for RSPO3 expression in the intestine (Kabiri et al, 2014). To verify the stromal origin of RSPO3 transcripts in CRC, we exploited the fact that in CRC patient‐derived xenografts (PDXs), human stroma is substituted by mouse stroma (Isella et al, 2015). Analysis of our previous species‐specific analysis of RNA‐seq data from CRC PDXs (Isella et al, 2015) revealed that the fraction of RSPO3 transcripts of mouse origin is 99.9%. As a consequence, it is expected that in a human CRC sample, RSPO3 mRNA levels are correlated with the richness of stroma that we found to be reliably approximated by a transcriptional ...
AXIL operates with a clear vision that innovation is the heart of its business. With the fast-paced level of product and technology expansion, AXIL has lead the way in providing the very best product designs and advancements to the sporting goods industry.. AXIL has and always will deliver the finest performing hearing enhancement and protection devices available. From ultra comfortable fitting processes, to stereo quality sound performance, to reliable & durable engineering - AXIL continues to redefine the premium products in the market.. AXIL is a family business heritage, with over 50 years of design, manufacturing and retailing experience. Todays AXIL team relies on this experience to deliver the highest quality and most advanced product line to the sporting goods market.. ...
J:169706 Abdul-Ghani M, Dufort D, Stiles R, De Repentigny Y, Kothary R, Megeney LA, Wnt11 promotes cardiomyocyte development by caspase-mediated suppression of canonical Wnt signals. Mol Cell Biol. 2011 Jan;31(1):163-78 ...
Otto Dix is a difficult artist to like, writes Judith Dobrzynski of the Weimar artist known for his harsh, cruel depictions of social and moral decay. But admiration is a different thing altogether.
Six people, two of them children, were injured in a two-car crash Monday night on the south service road of the Long Island Expressway in Dix Hills, a fire official said.The crash occurred at Deer Par
320 1H1012 - MemoryTen products carry a lifetime exchange or repair warranty against manufacturing defects. Products may also be returned in original condition within 14 days of delivery for full credit minus shipping. We also supply unlimited lifetime tech support for this item.
Axil Axil suggested in the Vortex discussion list - about the only list I read these days - the name nanoplasmonics for developments in cold fusion (while referencing a very funny mockery of how academics will revise the history of cold fusion in 2015 - History is written by the losers). The field is so…
本页面有Yongning Ventilation System Manufacturing Co.,Ltd.提供BT35 Blastproof Axil Blower相关产品及服务信息,产品名称主要以批发和零售为主,还包BT35 Blastproof Axil Blower、价格、BT35 Blastproof Axil Blower厂家等,想了解更加全面的BT35 Blastproof Axil Blower信息及产品信息就上Yongning Ventilation System Manufacturing Co.,Ltd.官网(http://yongh.qiyeku.cn)找更多BT35 Blastproof Axil Blower的信息!
Evidence to date indicates the predominance of activation of β-catenin/TCF-regulated target genes following Wnt pathway deregulation in the development and progression of cancer. The present study offers evidence that PLD1 drives a positive feedback loop to reinforce the Wnt/β-catenin/TCF signaling axis. We found that injection of LiCl into mice significantly increased the level of PLD1 protein in colon tissues (data not shown), suggesting its physiologic relevance in vivo. β-Catenin and TCF-4 elevated the expression and activity of PLD1. We were able to define three functional TCF binding sites within the PLD1 promoter, suggesting that the PLD1 gene is a direct transcriptional target of β-catenin/TCF signaling. We found that the mRNA level and promoter activity of PLD1 were diminished by wild-type ectopic expression of APC, axin, and GSK3β, whereas expression of Dvl3 and inactive mutant APC as positive regulators of Wnt signaling increased expression of PLD1 (data not shown). Thus, it is ...
G007-LK is a potent, rule of 5 compliant and a metabolically stable TNKS1/2 inhibitor. G007-LK displayed high selectivity toward tankyrases 1 and 2 with biochemical IC50 values of 46 nM and 25 nM, respectively, and a cellular IC50 value of 50 nM combined with an excellent pharmacokinetic profile in mice. The PARP domain of TNKS2 was cocrystallized with G007-LK and the X-ray structure was determined at 2.8 Ã… resolution in the space group P3221.
Diagnosis Antedonidae with centrodorsal conical, not higher than wide, with sockets in 10 columns, separated interradially by naked, shallow groove. Proximal brachials smooth, cylindrical, and not in lateral contact. Single known specimen has 14 arms; synarthry between primibrachials 1 and 2; secundibrachials with an axil at second or fourth ossicle or undivided; placement of syzygies depends on whether the secundibrachial series is part of the undivided arm (syzygy between secundibrachials 3 and 4) or terminates in an axil and ray division (no syzygy between secundibrachials 3 and 4). [details] ...
Infection with Helicobacter pylori is a major risk factor for the development of gastric cancer, and infection with strains carrying the virulence factor CagA significantly increases this risk. To investigate the mechanisms by which CagA promotes carcinogenesis, we generated transgenic zebrafish expressing CagA ubiquitously or in the anterior intestine. Transgenic zebrafish expressing either the wild type or a phosphorylation-resistant form of CagA exhibited significantly increased rates of intestinal epithelial cell proliferation and showed significant upregulation of the Wnt target genes cyclinD1, axin2 and the zebrafish c-myc ortholog myca. Coexpression of CagA with a loss-of-function allele encoding the β-catenin destruction complex protein Axin1 resulted in a further increase in intestinal proliferation. Coexpression of CagA with a null allele of the key β-catenin transcriptional cofactor Tcf4 restored intestinal proliferation to wild-type levels. These results provide in vivo evidence of ...
The Wnt/ß-catenin signaling pathway controls important cellular events during development and often contributes to disease when dysregulated. Using high throughput screening we have identified a new small molecule inhibitor of Wnt/ß-catenin signaling, WIKI4. WIKI4 inhibits expression of ß-catenin target genes and cellular responses to Wnt/ß-catenin signaling in cancer cell lines as well as in human embryonic stem cells. Furthermore, we demonstrate that WIKI4 mediates its effects on Wnt/ß-catenin signaling by inhibiting the enzymatic activity of TNKS2, a regulator of AXIN ubiquitylation and degradation. While TNKS has previously been shown to be the target of small molecule inhibitors of Wnt/ß-catenin signaling, WIKI4 is structurally distinct from previously identified TNKS inhibitors.
Cheeky coverage with self adjustable tie bottoms are it! Accentuate that booty while being family beach day appropriate in the Ellie bikini bottoms. Avail
Get info about Dix Hills NY licensed vocational nurse LVN jobs. Seek out accredited nursing programs for the best training. Given a brief hospital internship or prior work experience, you can expect a great return on your medical education.
Were looking for any eye-catching details like swirling grain, knots, evidence of any traumas the tree suffered long ago and for abnormalities in growth, Andy said, levering a piece of timber out. It was a ten-inch-wide, six-foot-long board of discoloured wood from the centre of my tree. There was detail in the figure, but it was difficult to make out. Andy said it was a carpenters hunch at this point: passing the board through the thicknessing machine would make the figure more visible, by neatly cutting the fibres that had previously been roughly torn by the saw at the sawmill ...
Plasmid pEMB36(revcomp)/Dvl1_1_670 from Dr. Michael J Fox Foundation MJFFs lab contains the insert DVL1 and is published in Unpublished This plasmid is available through Addgene.
Research Grants, Research Topics, Publications, Genomes and Genes, Species, Scientific Experts about adenomatous polyposis coli protein
Axon Medchem | Prime source supplier of high-value life science products, providing Axon Ligands™ for pharmaceutical research as world-wide recognized drug standards
Dylan Daniels and Molly Cavalli plays excellently in this amazing flick. Their lesbian lust flows all over the place and splashes out of the screen straight in your pants.
Strict Standards: Non-static method Registry::getInstance() should not be called statically in /home/alocalza/public_html/defaults_setup.php on line 198 ...
Blue Gartr Forums Built for the gamer inside all of us. Fresh to Elite, join our community supporting Final Fantasy, World of Warcraft, other MMOs, and all sorts of games
[Buda Adans. Fam. Pl. 2: 507. 1763.] [Spergularia Pers. Syn. 1: 504. 1805.] Low annual or perennial herbs, mostly with fleshy linear or setaceous leaves, often with others clustered in the axils...
Reliable plumbers are an important resource for every property owner. Axil Plumbing is licensed, insured, and trained to perform service in your home or business. Our locally owned company is based in Missoula and was started by master plumber Jason Templin.. Jason worked with a local plumbing company for more than 11 years before branching out to start Axil Plumbing, Heating & Air, LLC in May of 2014. Since then weve been busy building a business we can be proud of and spreading the word.. You can count on Axil Plumbing for fast, friendly, and flat-rate plumbing, heating and air conditioning needs. We specialize in boilers and on-demand hot water heaters and excel in advanced/high-efficient technology. We offer services for residential and light commercial properties and enjoy working with property managers and other local partners.. We make sure our customers are happy before we even start! Our upfront and flat-rate pricing takes away the guess work: we tell you the price and you say when to ...
Flowers: blue to purple, 5-parted, 1/4" long, very irregularly-shaped, slender spur at the base, 2 distinct lips with the lower one open at the throat and marked with yellow, 4 stamens; solitary, stalked flowers from the leaf axils; blooms June-Sept ...
I know that it is common to see a bit of spotting after a pelvic exam, but just wondering if mine is from my exam or an actual show? I am 38 weeks today and had the pelvic 2 days ago to check for dil...

Proteins matched: Axin beta-catenin binding (IPR014936) | InterPro | EMBL-EBIProteins matched: Axin beta-catenin binding (IPR014936) | InterPro | EMBL-EBI

We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ... Proteins matched: Axin beta-catenin binding (IPR014936) This domain is found in the following proteins: Showing 221 to 240 of ...
more infohttp://www.ebi.ac.uk/interpro/entry/IPR014936/proteins-matched?start=220

Proteins matched: Axin beta-catenin binding (IPR014936) filtered by species | InterPro | EMBL-EBIProteins matched: Axin beta-catenin binding (IPR014936) filtered by species | InterPro | EMBL-EBI

We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ... Proteins matched: Axin beta-catenin binding (IPR014936) Filtered by species: cellular organisms (includes child species) ( ...
more infohttp://www.ebi.ac.uk/interpro/entry/IPR014936/proteins-matched?taxonomy=131567

Low-density lipoprotein receptor-related protein-5 binds to Axin and regulates the canonical Wnt signaling pathway.  - PubMed -...Low-density lipoprotein receptor-related protein-5 binds to Axin and regulates the canonical Wnt signaling pathway. - PubMed -...

Low-density lipoprotein receptor-related protein-5 binds to Axin and regulates the canonical Wnt signaling pathway.. Mao J1, ... Addition of Wnt caused the translocation of Axin to the membrane and enhanced the interaction between Axin and LRP-5. In ... we identified Axin as a protein that interacts with the intracellular domain of LRP-5. LRP-5, when expressed in fibroblast ... Thus, we conclude that the binding of Axin to LRP-5 is an important part of the Wnt signal transduction pathway. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/11336703?dopt=Abstract

Axin1 - Axin-1 - Mus musculus (Mouse) - Axin1 gene & proteinAxin1 - Axin-1 - Mus musculus (Mouse) - Axin1 gene & protein

Pfam protein domain database. More...Pfami. View protein in Pfam. PF16646 AXIN1_TNKS_BD, 1 hit. PF08833 Axin_b-cat_bind, 1 ... ubiquitin protein ligase binding Source: BHF-UCLInferred from physical interactioni*. "Axin is a scaffold protein in TGF-beta ... positive regulation of protein ubiquitination Source: MGIInferred from direct assayi*. "Axin is a scaffold protein in TGF-beta ... protein-containing complex Source: MGIInferred from direct assayi*. "Axin is a scaffold protein in TGF-beta signaling that ...
more infohttps://www.uniprot.org/uniprot/O35625

axin1 - Axin-1 - Danio rerio (Zebrafish) - axin1 gene & proteinaxin1 - Axin-1 - Danio rerio (Zebrafish) - axin1 gene & protein

View protein in InterPro. IPR029797. AXIN1. IPR014936. Axin_b-cat-bd. IPR032101. Axin_TNKS-bd. IPR001158. DIX. IPR016137. RGS. ... View protein in InterPro. IPR029797. AXIN1. IPR014936. Axin_b-cat-bd. IPR032101. Axin_TNKS-bd. IPR001158. DIX. IPR016137. RGS. ... Pfam protein domain database. More...Pfami. View protein in Pfam. PF16646. AXIN1_TNKS_BD. 1 hit. PF08833. Axin_b-cat_bind. 1 ... Pfam protein domain database. More...Pfami. View protein in Pfam. PF16646. AXIN1_TNKS_BD. 1 hit. PF08833. Axin_b-cat_bind. 1 ...
more infohttp://www.uniprot.org/uniprot/P57094

The Regulator of G Protein Signaling Domain of Axin Selectively Interacts with Gα12 but Not Gα13 | Molecular PharmacologyThe Regulator of G Protein Signaling Domain of Axin Selectively Interacts with Gα12 but Not Gα13 | Molecular Pharmacology

GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins. Annu ... One subfamily of RGS proteins, the primary member of which is a protein termed axin, contains multiple domains that facilitate ... Known binding axin partners are indicated above the appropriate domain binding site. A, full-length axin; B, GFP-AxinΔDIX; C, ... A cocrystal structure of the RGS domain of axin with the axin-binding domain of APC has confirmed that the axin RGS domain is ...
more infohttp://molpharm.aspetjournals.org/content/70/4/1461

Axin interactor, dorsalization-associated proteinAxin interactor, dorsalization-associated protein

Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta ... Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein ... A beta-catenin-independent dorsalization pathway activated by Axin/JNK signaling and antagonized by aida.. ... independent dorsalization pathway activated by AXIN/JNK-signaling (By similarity). ...
more infohttps://pharos.nih.gov/idg/targets/Q96BJ3

GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Axin is a scaffold protein in TGF-beta signaling that promotes...GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Axin is a scaffold protein in TGF-beta signaling that promotes...

Axin is a scaffold protein in TGF-beta signaling that promotes degradation of Smad7 by Arkadia. ... AXIN1 BOS_22580] Axin 1 (Axin-1-like isoform 2). [AXIN1] Axin 1. [AXIN1] Axin 1. [AXIN1] Axin 1. [Axin1] Axin-1 (Axin1 protein) ... axin1 axin] Axin-1 (Axis inhibition protein 1). [Axn CG7926] Axin (Axis inhibition protein) (d-Axin) (dAxin). [AXIN1 AXIN AXN] ... AXIN1 AXIN] Axin-1 (Axis inhibition protein 1) (hAxin). [Axin1 Axin Fu] Axin-1 (Axis inhibition protein 1) (Protein Fused). [ ...
more infohttp://www.antibody-antibodies.com/pubmed-PubMed:16601693-PubMedArticl.html

MEDLINE - Resultado p gina 1
	MEDLINE - Resultado p gina 1

0 (Axin Protein); EC 2.4.2.30 (Tankyrases); EC 2.4.4.30 (TNKS protein, human); EC 3.1.2.15 (USP25 protein, human); EC 3.4.19.12 ... 0 (AXIN1 protein, human); 0 (AXIN2 protein, human); 0 (Axin Protein); 0 (G007-LK); 0 (Sulfones); 0 (Triazoles); 0 (beta Catenin ... 0 (AMOTL1 protein, human); 0 (AMOTL2 protein, human); 0 (Adaptor Proteins, Signal Transducing); 0 (Antineoplastic Agents); 0 ( ... Wnt signaling is negatively regulated by Axin, a scaffolding protein that controls a rate-limiting step in the destruction of - ...
more infohttp://bases.bireme.br/cgi-bin/wxislind.exe/iah/online/?IsisScript=iah/iah.xis&nextAction=lnk&base=MEDLINE&lang=p&format=detailed.pft&indexSearch=EX&exprSearch=D08.811.913.400.725.115.690.840

Similar articles for PubMed (Select 12636921) - PubMed - NCBISimilar articles for PubMed (Select 12636921) - PubMed - NCBI

Axin and the Axin/Arrow-binding protein DCAP mediate glucose-glycogen metabolism. ... Low-density lipoprotein receptor-related protein-5 binds to Axin and regulates the canonical Wnt signaling pathway. ... Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin. ... Regulation of the protein kinase activity of Shaggy(Zeste-white3) by components of the wingless pathway in Drosophila cells and ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?LinkName=pubmed_pubmed&from_uid=12636921

a) Immunoblot analysis of β-catenin, cyclin D1, cycli | Open-ia) Immunoblot analysis of β-catenin, cyclin D1, cycli | Open-i

Amyloid Precursor Protein Secretases. *Animals. *Aspartic Acid Endopeptidases. *Axin Protein. *Cyclin D1 ... Western blotting showed that PS1−/− cells contained higher amounts of β-catenin and cyclin D1 protein (Fig. 1 a). In contrast, ... Western blotting showed that PS1−/− cells contained higher amounts of β-catenin and cyclin D1 protein (Fig. 1 a). In contrast, ... In addition to its documented role in the proteolytic processing of Notch-1 and the beta-amyloid precursor protein, presenilin ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2195782_JCB0011002.f1c&req=4

LDL receptor-related proteins 5 and 6 in Wnt/β-catenin signaling: Arrows point the way | DevelopmentLDL receptor-related proteins 5 and 6 in Wnt/β-catenin signaling: Arrows point the way | Development

... and takes place in a protein complex assembled by the scaffolding protein Axin and the tumor suppressor protein Apc, the ... Axin-Lrp6 binding has not been mapped. Known binding sites for various Axin-binding proteins are depicted with square brackets ... Schematic diagram of Axin, showing its binding sites for various interacting proteins, including Arrow/Lrp5. How Axin binds to ... Fz-associated Dishevelled (Dvl) protein may bind Axin and inhibit Axin-Gsk3 phosphorylation ofβ -catenin, either directly or ...
more infohttp://dev.biologists.org/content/131/8/1663?ijkey=f3e491daa4e22bac74a0a20efc4a800a3239d753&keytype2=tf_ipsecsha

ZFIN Publication: Heisenberg et al., 2001ZFIN Publication: Heisenberg et al., 2001

Axin Protein. *Body Patterning/genetics*. *Body Patterning/physiology. *Calcium-Calmodulin-Dependent Protein Kinases/genetics* ... Here we show that mbl(-/-) embryos carry an amino-acid change at a conserved site in the Wnt pathway scaffolding protein, Axin1 ... The amino-acid substitution present in the mbl allele abolishes the binding of Axin to Gsk3 and affects Tcf-dependent ...
more infohttp://zfin.org/ZDB-PUB-010705-2

Axin | SpringerLinkAxin | SpringerLink

Coexpression and protein-protein complexing of DIX domains of human Dvl1 and Axin1 protein. BMB Rep. 2010;43:609-13. doi: ... Axin and GSK3- control Smad3 protein stability and modulate TGF- signaling. Genes Dev. 2008;22:106-20. doi:10.1101/gad.1590908. ... Axin is a scaffold protein in TGF-beta signaling that promotes degradation of Smad7 by Arkadia. EMBO J. 2006;25:1646-58. doi: ... Axin forms a complex with MEKK1 and activates c-Jun NH(2)-terminal kinase/stress-activated protein kinase through domains ...
more infohttps://link.springer.com/referenceworkentry/10.1007/978-1-4614-6438-9_101525-1

ZFIN Publication: Cheesman et al., 2011ZFIN Publication: Cheesman et al., 2011

Axin Protein. *Bromodeoxyuridine. *Cell Proliferation*. *Immunohistochemistry. *In Situ Hybridization. *Intestinal Mucosa/ ...
more infohttp://zfin.org/ZDB-PUB-101011-48

USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds | PNASUSP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds | PNAS

AXIN protein levels are negatively regulated by the poly(ADP)-ribosylation activity of the TNKS1/2 enzyme. Inhibiting TNKS1/2 ... results in increased AXIN protein levels and, consequently, decreased β-catenin activity. USP6-induced Wnt signaling was ... 2011) The ubiquitin-specific protease USP34 regulates axin stability and Wnt/β-catenin signaling. Mol Cell Biol 31(10):2053- ... 2G, basal as well as WNT3A-induced total β-catenin protein levels were elevated following USP6 overexpression. This increase in ...
more infohttps://www.pnas.org/content/113/21/E2945?ijkey=89684f671260530360ce9bf0aca8c52c3d6b7317&keytype2=tf_ipsecsha

Novel function of ∆Np63 in cell polarity and metabolism in pubertal mammary gland development.Novel function of ∆Np63 in cell polarity and metabolism in pubertal mammary gland development.

They contain an N-terminal dishevelled-AXIN PROTEIN (DIX) domain, which mediates oligomerization; a central PDZ DOMAIN which ... Dishevelled Proteins. A family of proteins that are key components of the WNT SIGNALING PATHWAY, where they function downstream ... Angiopoietin-like 4 Protein. A secreted angiopoietin-like protein expressed under hypoxic conditions by ENDOTHELIAL CELLS. It ... Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins ...
more infohttps://www.bioportfolio.com/resources/pmarticle/2551524/Novel-function-of-Np63-in-cell-polarity-and-metabolism-in-pubertal-mammary.html

The trimeric G protein Go inflicts a double impact on axin in the Wnt/frizzled signaling pathwayThe trimeric G protein Go inflicts a double impact on axin in the Wnt/frizzled signaling pathway

... Type of Publication:. Journal ... The trimeric G protein Go inflicts a double impact on axin in the Wnt/frizzled signaling pathway. * Home ... The trimeric G protein Go inflicts a double impact on axin in the Wnt/frizzled signaling pathway. In: Developmental Dynamics. ... Gαo physically binds the RGS domain of Axin and recruits Axin it to the plasma membrane, antagonizing the Axin-based ...
more infohttps://kops.uni-konstanz.de/handle/123456789/14815

JoVE | Peer Reviewed Scientific Video Journal - Methods and ProtocolsJoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols

Tankyrase (TNKS) is a poly-ADP-ribosylating protein (PARP) whose activity suppresses cellular axin protein levels and elevates ... They have been shown to directly bind to axin proteins, which negatively regulate the Wnt pathway by promoting ?-catenin ... At the protein level, a two-step strategy was taken using cells expressing His(6)-tagged ubiquitin, enriching proteins first ... To identify these substrates, we have taken both peptide and protein level approaches to enrich for ubiquitinated proteins in ...
more infohttps://www.jove.com/visualize?author=Paul%20S%20Andrews%20

Molecular Pathways: YAP and TAZ Take Center Stage in Organ Growth and Tumorigenesis | Clinical Cancer ResearchMolecular Pathways: YAP and TAZ Take Center Stage in Organ Growth and Tumorigenesis | Clinical Cancer Research

... and Axin proteins, thereby rescuing TAZ from degradation. Tankyrase inhibitors may reactivate Axin function and inhibit YAP/TAZ ... In keeping with the pro-YAP/TAZ role of Rho (31), recent results indicate that agonists of some G-protein-coupled receptors ( ... First, TAZ protein stabilization is an essential downstream effector of epithelial-to-mesenchymal transition (EMT) in breast ... Mst1 and Mst2 protein kinases restrain intestinal stem cell proliferation and colonic tumorigenesis by inhibition of Yes- ...
more infohttps://clincancerres.aacrjournals.org/content/19/18/4925?ijkey=10244011108c14ad7f4ca7630a909d944306379f&keytype2=tf_ipsecsha

Investigating the Influence of Hormone Signaling Pathways on Mammary Gland Development and the Onset of Breast Cancer | Learn &...Investigating the Influence of Hormone Signaling Pathways on Mammary Gland Development and the Onset of Breast Cancer | Learn &...

Axin2 is one of the Axin proteins which presumably play a role in the regulation of b-catenin stability. The protein b-catenin ... EGFP stands for Enhanced Green Fluorescence Protein, a protein that emits green when excited by blue light. In cell and ... Integrin β1 (CD29) and α6 (CD49f) are proteins that are integrin units. Integrin proteins are membrane receptors involved in ... cDNA is also used to express a specific protein in a cell that does not normally express that protein. ...
more infohttps://www.leica-microsystems.com/science-lab/investigating-the-influence-of-hormone-signaling-pathways-on-mammary-gland-development-and-the-onset-of-breast-cancer/

XAV939 inhibits TNKS PARP-activity in MB cell lines.WB  | Open-iXAV939 inhibits TNKS PARP-activity in MB cell lines.WB | Open-i

A. Both cell lines showed an increase in total Axin protein levels at 8 h after treatment (80% and 70% respectively in ONS-76 ... A. Both cell lines showed an increase in total Axin protein levels at 8 h after treatment (80% and 70% respectively in ONS-76 ... Mentions: XAV939 is known to induce WNT signaling inhibition by increasing Axin protein levels, thereby promoting β-catenin ... Mentions: XAV939 is known to induce WNT signaling inhibition by increasing Axin protein levels, thereby promoting β-catenin ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC4383513_pone.0124149.g001&req=4

Anti-CXXC4 antibody (ab105400) | AbcamAnti-CXXC4 antibody (ab105400) | Abcam

Dvl binding protein IDAX (inhibition of the Dvl and Axin complex) antibody ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ... Lysates/proteins at 15 µg per lane.. Predicted band size: 21 kDa. ... The Universal Protein Resource (UniProt) in 2010. Nucleic Acids Res. 38:D142-D148 (2010) . ...
more infohttps://www.abcam.com/cxxc4-antibody-ab105400.html

ELM - Browse/Search classesELM - Browse/Search classes

DOC_GSK3_Axin_1 Docking motif present in Axin protein binds the GSK-3β kinase and aids the phosphorylation of components in the ... A binding site for IRF-3 protein present in various innate adaptor proteins and the viral protein NSP1to trigger the innate ... The EEY/F$ motif is highly conserved and so far limited to a few known proteins, alpha-tubulin, EB proteins and CLIP170.. [ED ... This degron motif is present in Aux/IAA transcriptional repressor proteins and binds to TIR1/AFB F-box proteins of the SCF E3 ...
more infohttp://elm.eu.org/elms

Suppression of Slit3 induces tumor proliferation and chemoresistance in hepatocellular carcinoma through activation of GSK3β/β...Suppression of Slit3 induces tumor proliferation and chemoresistance in hepatocellular carcinoma through activation of GSK3β/β...

protein complex comprising of β-catenin, GSK-3β,APC These clinical findings in combination with the functional and AXIN ... protein complex comprising of β-catenin, GSK-3β,APC These clinical findings in combination with the functional and AXIN ... Protein expression of certain proteins associated with cell cycle gested that the GSK3β/β-catenin pathway served as a ... Protein expression of certain proteins associated with cell cycle gested that the GSK3β/β-catenin pathway served as a ...
more infohttps://www.deepdyve.com/lp/springer_journal/suppression-of-slit3-induces-tumor-proliferation-and-chemoresistance-Cs9Tu0TcPk
  • In the absence of Wnt ligand, cytosolic β-catenin is bound to a complex of proteins including axin, adenomatous polyposis coli (APC), and glycogen synthase kinase 3β, where it becomes phosphorylated, ubiquitinated, and directed to the proteosome for degradation. (aspetjournals.org)
  • PrxII deletion in mice with inactivating mutation of adenomatous polyposis coli (APC) gene reduces intestinal adenomatous polyposis via Axin/ -catenin axis and thereby promotes survival. (bireme.br)
  • To understand how the Wnt coreceptor LRP-5 is involved in transducing the canonical Wnt signals, we identified Axin as a protein that interacts with the intracellular domain of LRP-5. (nih.gov)
  • Herein, we demonstrate both in vitro and in cells that this domain interacts with the α subunit of the heterotrimeric G protein G 12 but not with the closely related Gα 13 or with several other heterotrimeric G proteins. (aspetjournals.org)
  • Wg/Wnt signal can be transmitted through arrow/LRP5,6 and Axin independently of Zw3/Gsk3beta activity. (nih.gov)
  • Pulse-chase experiments further illustrated that Axin significantly decreased the half-life of Smad7. (antibody-antibodies.com)
  • At the molecular level, Tankyrase inhibitors significantly decreased YAP protein levels, reduced the expression of YAP target genes, and inhibited YAP/TEAD luciferase reporter activity. (bireme.br)
  • Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (nih.gov)
  • Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse Axin 2. (abcam.com)
  • Here, we show that Axin activates TGF-beta signaling by forming a multimeric complex consisting of Smad7 and ubiquitin E3 ligase Arkadia. (antibody-antibodies.com)
  • This high affinity motif is required for the efficient recruitment of target proteins to the Cul3-based E3 ligase. (eu.org)
  • In addition, Tankyrase inhibitors administration was accompanied by upregulation of Angiomotin-like 1 (AMOTL1) and Angiomotin-like 2 (AMOTL2) proteins, two major negative regulators of YAP. (bireme.br)
  • Altogether, the present data indicate that XAV-939 and G007-LK Tankyrase inhibitors could suppress proliferation of hepatocellular carcinoma cells and downregulate YAP/TAZ by stabilizing AMOTL1 and AMOTL2 proteins, thus representing new potential anticancer drugs against hepatocellular carcinoma. (bireme.br)
  • While many components involved in the TGF-beta pathway have been elucidated, little is known about how those components are coordinated by scaffold proteins. (antibody-antibodies.com)
  • Cdk5-mediated phosphorylation of Axin directs axon formation during cerebral cortex development. (springer.com)
  • RNA-sequencing analysis reveals reduced expression of oxidative phosphorylation (OXPHOS)-associated proteins and desmosomal polarity proteins. (bioportfolio.com)
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • PSMG1 is a chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. (antibody-antibodies.com)
  • Here recent discoveries on the mechanism of how progesterone and its downstream signaling protein Wnt4, which promotes mammary gland development, control mammary stem cell function is reported. (leica-microsystems.com)
  • Based on the model, we can propose that controlling axin levels and its turnover is the major way by which the pathway can be regulated," Lee says. (vanderbilt.edu)
  • Interestingly, Axin associates with Arkadia and Smad7 independently of TGF-beta signal, in contrast to its transient association with inactive Smad3. (antibody-antibodies.com)
  • We found an effective increase in total Axin levels, 8 h after treatment with XAV939 (5 μM) and a decrease in β-catenin protein levels, both in total and in nuclear extracts, at 16 h. (nih.gov)
  • Densitometry analyses revealed a 70% increase of total Axin protein levels in DAOY cells and an 80% increase in ONS-76 cells, treated with XAV939 (5 μM) compared to DMSO treated control cells. (nih.gov)
  • Despite its inability to act as a GTPase-activating protein, we demonstrate that in cells, axinRGS can compete for Gα 12 binding with the RGS domain of p115RhoGEF, a known G 12 -interacting protein that links G 12 signaling to activation of the small G protein Rho. (aspetjournals.org)
  • These findings establish that the RGS domain of axin is able to directly interact with the α subunit of heterotrimeric G protein G 12 and provide a unique tool to interdict Gα 12 -mediated signaling processes. (aspetjournals.org)
  • The RGS domain of axin is the site on this protein at which binding of APC occurs. (aspetjournals.org)
  • This protein is contains a conserved, 120 amino acid motif called the RGS domain. (antibody-antibodies.com)
  • In addition to their GTPase-stimulating activities, some RGS proteins act as scaffolding molecules that hold signaling complexes together. (aspetjournals.org)
  • Ab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa. (abcam.com)
  • These Axin null mutants normally led to lethality at embryonic day 8-10, and in addition to causing embryonic axis duplications, they also led to. (springer.com)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • Axin depends on Arkadia to facilitate TGF-beta signaling, as their small interfering RNAs reciprocally abolished the stimulatory effect on TGF-beta signaling. (antibody-antibodies.com)