Axenic culture. Medical search

Axenic Culture: Culture of an isolated organism free from any other associating or contaminating organisms.Claviceps: A genus of ascomycetous fungi, family Clavicipitaceae, order Hypocreales, parasitic on various grasses (POACEAE). The sclerotia contain several toxic alkaloids. Claviceps purpurea on rye causes ergotism.Peptones: Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Myxomycetes: A division of organisms that exist vegetatively as complex mobile plasmodia, reproduce by means of spores, and have complex life cycles. They are now classed as protozoa but formerly were considered fungi.Entamoeba histolytica: A species of parasitic protozoa causing ENTAMOEBIASIS and amebic dysentery (DYSENTERY, AMEBIC). Characteristics include a single nucleus containing a small central karyosome and peripheral chromatin that is finely and regularly beaded.Eukaryota: One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Nitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Starvation: Lengthy and continuous deprivation of food. (Stedman, 25th ed)Microalgae: A non-taxonomic term for unicellular microscopic algae which are found in both freshwater and marine environments. Some authors consider DIATOMS; CYANOBACTERIA; HAPTOPHYTA; and DINOFLAGELLATES as part of microalgae, even though they are not algae.Iodides: Inorganic binary compounds of iodine or the I- ion.Photobioreactors: Devices for generating biological products that use light as the energy source. They are used for controlled BIOMASS production such as growing cyanobacteria, mosses, or algae.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Biofuels: Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).Chlorophyta: A phylum of photosynthetic EUKARYOTA bearing double membrane-bound plastids containing chlorophyll a and b. They comprise the classical green algae, and represent over 7000 species that live in a variety of primarily aquatic habitats. Only about ten percent are marine species, most live in freshwater.Nitrate Reductase (NAD(P)H): An iron-sulfur and MOLYBDENUM containing FLAVOPROTEIN that catalyzes the oxidation of nitrite to nitrate. This enzyme can use either NAD or NADP as cofactors. It is a key enzyme that is involved in the first step of nitrate assimilation in PLANTS; FUNGI; and BACTERIA. This enzyme was formerly classified as EC 1.6.6.2.Cladocera: A suborder of CRUSTACEA, order Diplostraca, comprising the water fleas. They are benthic filter feeders that consume PHYTOPLANKTON. The body is laterally compressed and enclosed in a bivalved carapace, from which the head extends.Lysobacter: A genus of gram-negative, rod-shaped, gliding bacteria in the family XANTHOMONADACEAE. Strongly proteolytic, it is involved in lysing a variety of microorganisms.Hydrocarbons Chara: A genus of green plants in the family CHARACEAE, phylum STREPTOPHYTA. They have a strong garlic-like odor and are an important food source for waterfowl.Tritrichomonas foetus: A species of flagellate parasitic EUKARYOTE. It possesses a long undulating membrane that is bordered on its outer margin by a flagellum that becomes free posteriorly. This organism causes infections in cows that could lead to temporary infertility or abortion.Tritrichomonas: A genus of flagellate EUKARYOTES possessing three long anterior flagella.Protozoan Infections, Animal: Infections with unicellular organisms formerly members of the subkingdom Protozoa. The infections may be experimental or veterinary.Trichomonas: A genus of parasitic flagellate EUKARYOTES distinguished by the presence of four anterior flagella, an undulating membrane, and a trailing flagellum.Protozoan Infections: Infections with unicellular organisms formerly members of the subkingdom Protozoa.Trichomonadida: An order of parasitic EUKARYOTES typically having four to six flagella. True cysts are known in very few species. Trichomonadida were formerly members of the class Zoomastigophora in the old five kingdom paradigm.Trichomonas vaginalis: A species of TRICHOMONAS that produces a refractory vaginal discharge in females, as well as bladder and urethral infections in males.Commerce: The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)Taxes: Governmental levies on property, inheritance, gifts, etc.Prescription Fees: The charge levied on the consumer for drugs or therapy prescribed under written order of a physician or other health professional.Philately: Study of stamps or postal markings. It usually refers to the design and commemorative aspects of the stamp.Great Britain GermanyMedicine, Arabic: Traditional Arabic methods used in medicine in the ARAB WORLD.Western Australia: A state in western Australia. Its capital is Perth. It was first visited by the Dutch in 1616 but the English took possession in 1791 and permanent colonization began in 1829. It was a penal settlement 1850-1888, became part of the colonial government in 1886, and was granted self government in 1890. (From Webster's New Geographical Dictionary, 1988, p1329)Telefacsimile: A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.Genome, Mitochondrial: The genetic complement of MITOCHONDRIA as represented in their DNA.Sheep: Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.Agricultural Irrigation: The routing of water to open or closed areas where it is used for agricultural purposes.DNA, Mitochondrial: Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.Problem Solving: A learning situation involving more than one alternative from which a selection is made in order to attain a specific goal.Skin Manifestations: Dermatologic disorders attendant upon non-dermatologic disease or injury.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Genetics, Medical: A subdiscipline of human genetics which entails the reliable prediction of certain human disorders as a function of the lineage and/or genetic makeup of an individual or of any two parents or potential parents.Plant Cells: Basic functional unit of plants.Confusion: A mental state characterized by bewilderment, emotional disturbance, lack of clear thinking, and perceptual disorientation.Vocabulary, Controlled: A specified list of terms with a fixed and unalterable meaning, and from which a selection is made when CATALOGING; ABSTRACTING AND INDEXING; or searching BOOKS; JOURNALS AS TOPIC; and other documents. The control is intended to avoid the scattering of related subjects under different headings (SUBJECT HEADINGS). The list may be altered or extended only by the publisher or issuing agency. (From Harrod's Librarians' Glossary, 7th ed, p163)Systematized Nomenclature of Medicine: Controlled vocabulary of clinical terms produced by the International Health Terminology Standards Development Organisation (IHTSDO).Ethical Review: A formal process of examination of patient care or research proposals for conformity with ethical standards. The review is usually conducted by an organized clinical or research ethics committee (CLINICAL ETHICS COMMITTEES or RESEARCH ETHICS COMMITTEES), sometimes by a subset of such a committee, an ad hoc group, or an individual ethicist (ETHICISTS).

Leishmania major parasite stage-dependent host cell invasion and immune evasion. (1/14)

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Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota. (2/14)

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The genetic toolbox for Leishmania parasites. (3/14)

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Epidemiologic consequences of microvariation in Mycobacterium tuberculosis. (4/14)

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Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi. (5/14)

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Targeting host syntaxin-5 preferentially blocks Leishmania parasitophorous vacuole development in infected cells and limits experimental Leishmania infections. (6/14)

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Antileishmanial activity of warifteine: a bisbenzylisoquinoline alkaloid isolated from Cissampelos sympodialis Eichl. (Menispermaceae). (7/14)

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The response of dark septate endophytes (DSE) to heavy metals in pure culture. (8/14)

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Phytoplasma

Contaldo, N.; Bertaccini, A.; Paltrinieri, S.; Windsor, H.M.; Windsor, G.D. (2012). "Axenic culture of a plant pathogenic ... Tissue culture can be used to produce healthy clones of phytoplasma-infected plants. Cryotherapy (i.e., the freezing of plant ... status (used for bacteria that cannot be cultured). Phytoplasma taxonomy is complicated because the organisms cannot be ... and cannot be cultured. Viral and phytoplasmic infections share some symptoms. In 1967, phytoplasmas were discovered in ...

Vernalization

Sokolski, K.; Dovholuk, A.; Dovholuk, L.; Faletra, P. (1997). "Axenic seed culture and micropropagation of Cypripedium reginae ...

Lycopodium obscurum

Gametophytes of Lycopodium obscurum as grown in axenic culture. Canadian Journal of Botany. 55. pp. 563-567. Hickey, R. James. ...

Gnotobiosis

Specific-pathogen-free (SPF) Axenic culture Reyniers JA (1959). "Germfree Vertebrates: Present Status". Annals of the New York ...

Endogone

Berch SM, Castellano MA (1986). "Sporulation of Endogone pisiformis in axenic and monaxenic culture". Mycologia. 78 (2): 292- ... 1990). "Taxonomy, culture, and mycorrhizal associations of some zygosporic Endogonaceae". Mycological Research. 94 (2): 173-178 ... pisiformis can be made to sporulate in test tube culture when grown with conifer seedlings. Endogone was first circumscribed by ...

Tylopilus felleus

The mycelium of Tylopilus felleus can be grown in axenic culture, on agar containing growth medium. The fungus can form fruit ... 1974). "Fruitbody formation by Tylopilus fellus in axenic culture". Mycologia. 66 (1): 197-202. doi:10.2307/3758474. JSTOR ... There are few Boletaceae species known to fruit in culture, as ectomycorrhizal fungi tend to not fruit when separated from ...

Black bread mold

Baker, KL; Beneke, ES; Hooper, GR; Fields, WG (1977). "Host Range and Axenic Culture of the Mycoparasite Syncephalis sphaerica ...

Caenorhabditis briggsae

This work was important in the development of axenic culture methods. C. briggsae can often be found in compost, garden beds, ...

Limnoperdon

The tips of the hyphae were used to obtain axenic cultures; the fungus can grow on a variety of media commonly used to grow ... Escobar grew cultures of the fungus by placing fresh fruit bodies on agar containing growth medium with an extract of horse ... Experimental observations on the development of the fruit body, based on the growth on the fungus in pure culture, suggest that ... Mycologist Dennis McCabe studied the development of the fruit body using specimens grown in pure culture. Starting out as a ...

Symbiodinium

I. Isoenzyme and Soluble Protein Patterns of Axenic Cultures of Symbiodinium microadriaticum". Proceedings of the Royal Society ... In culture, the discarded walls accumulate at the bottom of the culture vessel. It is not known what becomes of the walls from ... All cultured isolates (i.e. strains) are capable of phenotypic adjustment in their capacity for light harvesting (i.e. ... As cultures reach stationary growth phase, fewer and fewer motile cells are observed, indicating slower division rates. Large ...

Cypripedium reginae

It was difficult to raise from seed, taking many months to germinate in sterile culture until progress on axenic culture from ... 1997). "Axenic Seed Culture and Micropropagation of Cypripedium reginae" Selbyana 18(2): 172-182. Correll, Donovan (1978) [1950 ... in Axenic Seed Culture, Bulletin of America Association for the Advancement of Sciences, Annual meeting, pp. A-112 AAAS Annual ... in Axenic Seed Culture. Walsh, V.; N. Ramos; P. Faletra (2013). "Characterization of Cypripedium species and Habitats in New ...

Spanish Bank of Algae

These cultures have strain numbers preceded by BEA. Non-axenic cultures are marked with "B" at the end of the strain number. ... BEA has been member of the European Culture Collection Organization (ECCO) since 2001 and of the World Federation for Culture ... "Spanish Type Culture Collection (University of Valencia)" (in Spanish). Retrieved 26 December 2011. "European Culture ... gDNA "à la carte": BEA is able to extract genomic DNA from microalgae cultures from a wide range of volumes. Normally kits (i.e ...

Aspergillus austroafricanus

"Metabolites from the Fungal Endophyte Aspergillus austroafricanus in Axenic Culture and in Fungal-Bacterial Mixed Cultures". ...

Phaeocystis

Production of DMS from dissolved DMSP in axenic cultures of the marine phytoplankton species Phaeocystis sp., Mar. Ecol. Prog. ...

Chloroflexi (phylum)

Prokaryotes where no pure (axenic) cultures are isolated or available; i.e., they are not cultivated or cannot be sustained in ...

Phycotoxin

... multiseries cultures which were completely axenic (bacteria-free), produce less domoic acid than P. multiseries cultures which ... "Enhancement of domoic acid production by reintroducing bacteria to axenic cultures of the diatom Pseudo-nitzschia multiseries ... For example, in a mixed batch culture the target species may die or have reduced growth rates due to competition for nutrients ... All methods of detecting phycotoxins involve extraction of the candidate toxin from a phytoplankton culture; therefore, it's ...

Root-knot nematode

"Effect of ammonium ions on egg hatching and second-stage juveniles of Meloidogyne incognita in axenic tomato root culture". ...

Bartonella quintana

It was first isolated in axenic culture by J.W. Vinson in 1960, from a patient in Mexico City. He then followed Koch's ... Release of an icosahedral particle, 40 nm in length, has been detected in cultures of B. quintana's close relative, B. henselae ... To have a definite diagnosis of infection with B. quintana requires either serological cultures or nucleic acid amplification ...

Candidate division TM7

An axenic culture of TM7 from the oral cavity was reported in 2014 but no sequence or culture was made available. Along with ... "Axenic Culture of a Candidate Division TM7 Bacterium from the Human Oral Cavity and Biofilm Interactions with Other Oral ... a Recently Recognized Major Lineage of the Domain Bacteria with No Known Pure-Culture Representatives". Applied and ...

Moss bioreactor

The culture is kept under lighting with temperature and pH value held constant. The culture medium-often a minimal medium- ... Algae bioreactor Bioreactor Molecular farming Photobioreactor Physcomitrella patens Hohe, A.; Reski, R. (2005). "From axenic ... "Production of a heterologous protein in bioreactor cultures of fully differentiated moss plants" (PDF). Plant Tissue Culture ... At this point the culture has to be diluted with fresh medium if it is intended for further use. According to the intended ...

History of research on Caenorhabditis elegans

... of the early laboratory work on Caenorhabditis nematodes was directed towards the establishment of a defined axenic culture ... Ellsworth C. Dougherty; Eder Lindsay Hansen; Warwick L. Nicholas; J. Anthony Mollett; Evangeline A. Yarwood (1959). "Axenic ... from a laboratory culture of the closely related nematode Caenorhabditis briggsae. However much ...

Donal T. Manahan

Amino acid uptake and metabolism by larvae of the marine worm Urechis caupo (Echiura), a new species in axenic culture. ...

Germ-free animal

Axenic culture University of Michigan Germ Free Animal Facility - example facility for raising germ-free animals Reyniers, J.A ...

Microbiological culture

It is often essential to isolate a pure culture of microorganisms. A pure (or axenic) culture is a population of cells or ... Stab cultures are most commonly used for short-term storage or shipment of cultures. Microbial culture collections focus on the ... Blood culture International Collection of Microorganisms from Plants Microbial Food Cultures NCPPB Screening cultures Sputum ... the isolation of pure cultures uses the same techniques as for bacterial cultures. Pure cultures of multicellular organisms are ...

Vampirovibrio chlorellavorus

V. chlorellavorus will not grow in axenic cultures, depending on access to living cells of its preferred algae host, Chlorella ... Since Vampirovibrio chlorellavorus has not been cultured in recent years, it is possible to learn about its future research ... Subsequently, Soo and Hugenholtz's team performed a genomic reconstruction in 2014 from a culture previously deposited into the ... chlorellavorus in a lysis experiment with the algae Chlorella vulgaris from Ukrainian reservoir waters from a mass culture of ...

Amoebidiidae

... of the host and grow in axenic culture, at least some species may be facultative symbionts. The "amoeb-" prefix refers to the ...

Intracellular parasite

However, in 2009 scientists reported a technique allowing the Q-fever pathogen Coxiella burnetii to grow in an axenic culture ...

Bacteroidetes

Prokaryotes where no pure (axenic) cultures are isolated or available, i.e., not cultivated or cannot be sustained in culture ... "The first 1000 cultured species of the human gastrointestinal microbiota". FEMS Microbiology Reviews. 38 (5): 996-1047. doi ...

AXENIC INOCULATION SYSTEM FOR MICROALGAE USING TR TUBE OF TRIIODIDE RESIN AND METHOD FOR CULTURING AXENIC CULTURE - Patent...

... thereby obtaining an axenic culture; and inoculating the axenic culture into a culture liquid inside the PBR to culture ... inoculate the axenic culture in a culture solution inside the PBR to culture microalgae. 10. The axenic inoculation system ... installed at an upper portion of the PBR to obtain an axenic culture, and then, inoculating the axenic culture in a culture ... installed at the upper portion of the PBR to obtain an axenic culture; and inoculate the axenic culture in a culture solution ...

patentsencyclopedia.com

Tritrichomonas foetus (Riedmuller) Wenrich and Emerson ATCC ® 3023

Culture System: Axenic. Subcultivation Protocol: at pH 7.0. Cryopreservation Harvest and Preservation *Harvest cells from a ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just ... Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal ...

atcc.org

"CLUPS": A New Culture Medium for the Axenic Growth of Entamoeba...

Culture Strains. Trophozoites from the E. histolytica HM1: IMSS strain were cultured in TYI-S-33 medium under axenic conditions ... "CLUPS": A New Culture Medium for the Axenic Growth of Entamoeba histolytica. F. Gonzalez-Salazar,1,2 I. Meester,2 F. J. Guzmán ... We introduce a new culture medium (CLUPS) for the axenic growth of E. histolytica. CLUPS medium is a modification of the PEHPS ... Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, ...

hindawi.com

Axenic culture | Article about axenic culture by The Free Dictionary

Find out information about axenic culture. The growth of organisms of a single species in the absence of cells or living ... organisms of any other species Explanation of axenic culture ... Related to axenic culture: pure culture, monoxenic culture. ... Axenic culture , Article about axenic culture by The Free Dictionary https://encyclopedia2.thefreedictionary.com/axenic+culture ... In a re-association experiment, cells from the heterotrophic culture were added to an axenic culture of the Anabaena sp.. ...

encyclopedia2.thefreedictionary.com

Biodegradation of naphthenic acid surrogates by axenic cultures | Springer for Research & Development

Of the three cultures, Ochrobactrum showed most metabolic activity on the widest range of NA surrogates. Brevundomonas and ... These pure cultures were screened for their ability to use a range of aliphatic, cyclic and aromatic NA surrogates in 96-well ... Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor ...

rd.springer.com

The Nematode C. elegans as an Animal Model to Explore Toxicology In Vivo: Solid and Axenic Growth Culture Conditions and...

The Nematode C. elegans as an Animal Model to Explore Toxicology In Vivo: Solid and Axenic Growth Culture Conditions and ... Axenic culture of free‐living, parasitic and insect‐parasitic nematodes. Annu. Rev. Phytopathol. 16:131‐157. ... This unit includes protocols for culturing worms on solid and axenic media and acute and chronic exposure parameters for ... Use of CeHR Axenic Medium for Exposure and Gene Expression Studies. Reproductive Hazards Laboratory, U.S. Army Center for ...

currentprotocols.com

Cd and Zn interactions and toxicity in ectomycorrhizal basidiomycetes in axenic culture [PeerJ]

Methods A wide range of Cd and Zn concentrations were applied to ectomycorrhizal fungi in axenic cultures (in mg L−1): 0; 1; 3 ... Cd and Zn interactions and toxicity in ectomycorrhizal basidiomycetes in axenic culture. Vinicius H. De Oliveira, Mark Tibbett ... A wide range of Cd and Zn concentrations were applied to ectomycorrhizal fungi in axenic cultures (in mg L−1): 0; 1; 3; 9; 27; ... De Oliveira VH, Tibbett M. (2018) Cd and Zn interactions and toxicity in ectomycorrhizal basidiomycetes in axenic culture. ...

peerj.com

Frontiers | Growing poplars for research with and without mycorrhizas | Plant Science

Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are ... Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are ... Sand Cultures Under Axenic Conditions. Rooted poplars can also be grown in sand as the supporting medium under axenic ... Cocultivation Under Axenic Conditions. Culture systems for the cultivation of poplars with L. bicolor or P. involutus under in ...

frontiersin.org

Genetic Engineering of Trypanosoma (Dutonella) vivax and In Vitro Differentiation under Axenic Conditions

The work presented herein focused on determining axenic conditions for culturing and growing insect (epimastigote) forms of T. ... Axenic culture Is the Subject Area "Axenic culture" applicable to this article? Yes. No. ... Establishment of T. vivax axenic cultures and impact of serum source. We started T. vivax adaptation to axenic cultures using ... Parasite maintenance in axenic cultures and in vitro differentiation. Epimastigote cultures were initiated with the blood of ...

journals.plos.org

D in axenic cultures under conditions of nitrogen starvation [13,14], and at | esiservizi.com

D in axenic cultures under conditions of nitrogen starvation , and at least two of these ?SPM1 encoding a serine protease ... D in axenic cultures under conditions of nitrogen starvation [13,14], and at. Posted on July 24, 2017. by Proteasome inhibitor ... D in axenic cultures under conditions of nitrogen starvation [13,14], and at least two of these ?SPM1 encoding a serine ... Moreover, the up.D in axenic cultures under conditions of nitrogen starvation [13,14], and at least two of these ?SPM1 encoding ...

esiservizi.com

Frontiers | PCR and Omics Based Techniques to Study the Diversity, Ecology and Biology of Anaerobic Fungi: Insights, Challenges...

Application of genomics, transcriptomics, proteomics and metabolomics to their study in pure/mixed cultures and environmental ... with further novel taxonomic groupings known to exist based on culture-independent molecular surveys. However, the true extent ... with further novel taxonomic groupings known to exist based on culture-independent molecular surveys. However, the true extent ... Changes in fermentation end products of anaerobic fungal metabolism in axenic culture compared to co-culture with methanogens, ...

frontiersin.org

Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case

Axenic Isolates. Fungi and bacteria-free plates were transferred to axenic culture by placing the amoebae in PYG medium as ... DNA from amoebic cultures was extracted by placing 1-2 mL of axenic Acanthamoeba cultures directly into the Maxwell 16 Tissue ... Axenic Cultures. Monoxenic cultures were selected from areas of profuse amoebic growth. Selected pieces of agar were ... Briefly, axenic cultures of A. griffini trophozoites in the exponential phase of growth (72 h) were chilled at 4°C and ...

hindawi.com

Ankistrodesmus braunii Brunnthaler ATCC ® 30447™

Culture System: Axenic. Cryopreservation Harvest and Preservation *Harvest cells from a culture that is at or near peak density ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just ... Incubate the culture at 50-100 µEinsteins/m2/s irradiance at 25°C. Maintain under a 14/10h light-dark photoperiod. ...

atcc.org

Acanthamoeba polyphaga (Puschkarew) Page ATCC ® PRA-79™

Culture System: Axenic. Cryopreservation Harvest and Preservation *To achieve the best results, set up cultures with several ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... Harvest cultures and pool when the culture that received the lowest inoculum is at or near peak density. ... To establish a culture from the frozen state, place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just ...

atcc.org

Zeitschrift für Naturforschung C

Growth and Terpenoid Production of an Axenic Culture from the Liverwort Ricciocarpos natans. Wurzel, Gerald / Becker, Hans ...

degruyter.com

Aquatic plants stimulate the growth of and biofilm formation by Mycobacterium ulcerans in axenic culture and harbor these...

Aquatic plants stimulate the growth of and biofilm formation by Mycobacterium ulcerans in axenic culture and harbor these ...

findanexpert.unimelb.edu.au

Sheep mitochondrial genome

Previous message: axenic cultures *Next message: Searching.. * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] ...

bio.net

Use of Shell-Vial Cell Culture Assay for Isolation of Bacteria from Clinical Specimens: 13 Years of Experience | Journal of...

PCR before sample culture. Axenic culture. Cell culture detection at day 21. ... Characteristics of 27 microorganisms only isolated in the shell-vial culture by the JNSP protocol for which axenic cultures ... Presented for each microorganism are the results of 16S rRNA PCR, culture, and strain establishment in axenic medium. ... of positive shell-vial cultures. Species. No. of strains established from shell-vial culture. ...

jcm.asm.org

Metabolites | Free Full-Text | Volatile Metabolites Emission by In Vivo Microalgae-An Overlooked Opportunity? | HTML

The phytoplankton cultures examined were, Prochlorococcus (axenic and xenic), Synechococcus (axenic), Emiliania huxleyi, ... All cultures were axenic except C. leptoporous. The cultures were grown at 20-25 °C (depending on species) in an even split ... All cultures were axenic, with the exception of Porphyridium sp. and Prorocentrum sp. Cultures were bubbled with ultra-high ... It was unclear whether the cultures were axenic. Scarratt and Moore [92] examined cultures of several marine phytoplanktons: ...

mdpi.com

Equipment

Axenic cultures of symbiotic algae. *Modulated fluorescence of chlorophyll, kinetics (fluorometer, actinic light) ... Microbial cultures. Development of bioreactors under thermal insulation; genetic characterization and enzymatic analysis of ...

uv.es

Axenic - Wikipedia

The earliest axenic cultures were of bacteria or unicellular eukaryotes, but axenic cultures of many multicellular organisms ... identifying cultures that are no longer axenic. As axenic cultures are derived from very few organisms, or even a single ... Axenic culture is also an important tool for the study of symbiotic and parasitic organisms in a controlled manner. Axenic ... Axenic cultures are usually checked routinely to ensure that they remain axenic. One standard approach with microorganisms is ...

en.wikipedia.org

Combined culture and metagenomic analyses reveal significant shifts in the composition of the cutaneous microbiome in psoriasis.

Axenic Culture. Culture of an isolated organism free from any other associating or contaminating organisms. ... A shift from culture-dependent to culture-independent ide.... Metagenomic analyses of the gut microbiota associated with ... Culture. A collective expression for all behavior patterns acquired and socially transmitted through symbols. Culture includes ... Myriads of elements affect human embryo culture in vitro. These include, but not limited to, air quality, culture media, ...

bioportfolio.com

Q Fever Publications | PubFacts

Use of Axenic Culture Tools to Study Coxiella burnetii.. Authors:. Savannah E Sanchez Eduardo Vallejo-Esquerra Anders Omsland ... Lipid A Has Significance for Optimal Growth of in Macrophage-Like THP-1 Cells and to a Lesser Extent in Axenic Media and Non- ... Here we report the characterization of the role of lipid A in growth in axenic media, monkey kidney cells (BGMK and Vero), and ... Molecular detection of Coxiella burnetii in heart valve tissue from patients with culture-negative infective endocarditis.. ...

pubfacts.com

Basidiomata - definition of Basidiomata by The Free Dictionary

Pegler in axenic culture and obtained successful basidiomata production.. Production of Panus strigellus spawn using the ... Development in vitro of the symbiotic fungus of Atta sexdens rubropilosa Forel, 1908 (Hymenoptera: Formicidae) in culture ...

thefreedictionary.com

Marine Drugs | Free Full-Text | Antibacterial Activity of Marine and Black Band Disease Cyanobacteria against Coral-Associated...

Cultures of unicellular cyanobacterial isolates were axenic.. Heterotrophic bacteria used as test strains were isolated from ... Pure cultures were maintained at room temperature on marine agar slants, while cyanobacterial cultures were maintained on ... All filamentous cyanobacteria were cultured from individual trichomes and were uni-algal cultures. Accompanying bacteria were ... were obtained as non-axenic uni-algal cultures as previously described [33,37]. The three BBD cyanobacterial genera have been ...

mdpi.com

Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal slant for medium 2154). (atcc.org)

Preparation of Axenic Cultures of Algae by Use of a French Press" (PDF). (wikipedia.org)
The culture collection of algae at Indiana University. (atcc.org)
This project will focus primarily on laboratory cultures of axenic marine algae. (epa.gov)
A generalized set of conditions for culturing micro-algae (modified from Anonymous, 1991). (fao.org)
Algae without HKB upregulated their expression of genes coding for ammonium transporters, implying uptake of inorganic nitrogen from the culture medium when prey were unavailable. (biomedcentral.com)

donovani strain MHOM/ET/67/L82 were grown in axenic culture at 37 [degrees]C in SM medium (Cunningham 1977) at pH 5. (thefreedictionary.com)
The cultures are grown in a sterile synthetic liquid medium devoid of enriched supplements such as serum. (cdc.gov)
Axenic Mtb cultures were grown in Sauton's broth, a defined, minimal medium, to late-log phase. (nih.gov)
Microbiological cultures can be grown in petri dishes of differing sizes that have a thin layer of agar-based growth medium. (wikipedia.org)
This study aims to compare the phytochemical composition and pharmacological properties of axenically and naturally grown liverwort L. cruciata to find out whether the axenically cultured bryophytes can be used as a substitute to naturally grown ones to meet up the demands of sample available for use in research purposes. (biomedcentral.com)
Healthy stems and leaves of ten, 30-day-old plants of S. tragus were spray-inoculated with an aqueous suspension of conidia (1.0 x 106 alpha conidia/ml plus 0.1 percent v/v polysorbate 20) harvested from 14-day-old cultures grown on 20 percent V8 juice agar. (usda.gov)
Healthy stems and leaves of ten, 30-day-old plants of S. tragus were spray-inoculated with an aqueous suspension of conidia (1.0 x 106 alpha conidia/ml) harvested from 14-day-old cultures grown on 20 percent V8 juice agar. (usda.gov)
A. castellanii trophozoites were grown under axenic conditions to establish a cyst-free culture. (arvojournals.org)

Activities of phenol oxidizing enzymes of ectomycorrhizal fungi in axenic culture and in symbiosis with Scots pine (Pinus sylvestris L. (thefreedictionary.com)

Axenic cultivation of E. histolytica was first accomplished by Diamond in 1961 [ 9 ], using a diphasic medium composed of a serum-enriched agar base covered by a broth supplemented with chicken extract and vitamins. (hindawi.com)
In obtaining a culture from another laboratory, the proper designation of the culture, as originally named and described, must be maintained and any deviations in cultivation from the original must be reported in any publication. (sivb.org)

Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are described. (frontiersin.org)

They are digenetic parasites: the flagellated promastigote forms, which can be derived in axenic culture, differentiate within the alimentary tracts of sandfly vectors from a replicating procyclic to a nonreplicating, infectious metacyclic stage, whereas obligately intracellular amastigotes live and replicate in mammalian mononuclear phagocytes, such as macrophages ( 1 ). (asm.org)
We further demonstrated that reduction of CK1 activity by specific inhibitors, such as D4476, blocks promastigote growth, strongly compromises axenic amastigote viability, and decreases the number of intracellular Leishmania donovani and L. amazonensis amastigotes in infected macrophages. (asm.org)

One standard approach with microorganisms is to spread a sample of the culture onto an agar plate, and to incubate this for a fixed period of time. (wikipedia.org)
For the purpose of gelling the microbial culture, the medium of agarose gel ( agar ) is used. (wikipedia.org)
Stab cultures are similar to agar plates, but are formed by solid agar in a test tube. (wikipedia.org)

Axenic cultures of microorganisms are typically prepared by subculture of an existing mixed culture. (wikipedia.org)
For example, a throat culture is taken by scraping the lining of tissue in the back of the throat and blotting the sample into a medium to be able to screen for harmful microorganisms, such as Streptococcus pyogenes , the causative agent of strep throat. (wikipedia.org)
It is often essential to isolate a pure culture of microorganisms. (wikipedia.org)
Microbial culture collections focus on the acquisition, authentication, production, preservation, catalogueing and distribution of viable cultures of standard reference microorganisms , cell lines and other materials for research in microbial systematics . (wikipedia.org)

The present invention relates to an axenic inoculation system for microalgae using a triiodide resin (TR) tube of a triiodide resin, and a method for culturing an axenic culture. (patentsencyclopedia.com)

About 100 [micro]l of the culture medium with 105 amastigotes from axenic culture with or without a serial drug dilution was seeded in 96-well microtiter plates. (thefreedictionary.com)

A morphological description must await achieving axenic culture , but the partial sequence of the SSUrDNA has been given the GenBank accession numbers AF228362 and AF228363 for the V1, and V2 and V3 regions of the SSUrDNA, respectively. (thefreedictionary.com)
Cultures are authenticated using morphological and molecular techniques. (atcc.org)

The work presented herein focused on determining axenic conditions for culturing and growing insect (epimastigote) forms of T. vivax and prompting their differentiation into metacyclic forms that are infectious for the mammalian host. (plos.org)
Although culturing plant tissues and organs under axenic conditions was first established and profitably employed in bryophytes, especially mosses [ 13 ], bryophytes did not retain for long their rightful place as a highly favored research object. (biomedcentral.com)

A cell line arises from a primary culture at the time of the first successful subculture. (sivb.org)

To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters. (hindawi.com)
Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. (hindawi.com)

Microbial cultures. (uv.es)
Although these procedures are used to prevent microbial contamination of cultures, they also prevent cross contamination of cell cultures as well. (sivb.org)
Microbial cultures are foundational and basic diagnostic methods used extensively as a research tool in molecular biology . (wikipedia.org)
Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both. (wikipedia.org)
Anaerobic agents are the most difficult to detect from oxygen-containing environments and, therefore, it is critical that anaerobic collection culture media, anaerobic chambers, and powerful microbial identification technologies be utilized to ensure accurate results. (criver.com)

To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. (atcc.org)
To establish a culture from the frozen state, place an ampule in a water bath set at 35°C (2-3 min). (atcc.org)

A completely axenic C. burnetii genetic transformation system was developed using ACCM-2 that allowed isolation of transformants in about 2 1/2 weeks. (asm.org)

The axenic clinical isolates were cytopathogenic to rabbit corneal fibroblasts. (biomedcentral.com)

Molecular detection of Coxiella burnetii in heart valve tissue from patients with culture-negative infective endocarditis. (pubfacts.com)
Coxiella burnetii is a common cause of blood culture-negative infective endocarditis (IE). (pubfacts.com)

Culture media are undefined because they contain mammalian serum, which is a rich source of complex lipids and proteins. (figshare.com)

The recent description of a method for axenic (host cell-free) propagation of C. burnetii is a significant technological advance that will facilitate biochemical and genetic studies of the pathogen ( 22 ). (asm.org)
A pure culture may originate from a single cell or single organism, in which case the cells are genetic clones of one another. (wikipedia.org)

Here, we examined the diagnostic utility of Q fever polymerase chain reaction (PCR) of formalin-fixed heart valve tissue from patients with blood culture-negative IE who underwent heart valve surgery. (pubfacts.com)

This unit includes protocols for culturing worms on solid and axenic media and acute and chronic exposure parameters for Parkinson's disease-associated toxins and hemin chloride. (currentprotocols.com)

Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. (hindawi.com)
Various specific recipes for algal culture media are described by Vonshak (1986). (fao.org)
The complexity and cost of the above culture media often excludes their use for large-scale culture operations. (fao.org)

Diagnosis is based on serologic analysis, culture, and molecular biology. (cdc.gov)

Such diseases were originally thought to be caused by viruses, which, like phytoplasmas, require insect vectors, and cannot be cultured. (wikipedia.org)

1978. Method for the simultaneous establishment of many axenic cultures of Paramecium . (springer.com)

In cell cultures, the cells are no longer organized into tissues. (sivb.org)

they were cultured without flagellates in the first phase of the experiments and in the presence of the flagellates in the second phase. (asm.org)
In monolayer culture experiments, DH1-derived gskA -null cells do not display the cAMP repression of stalk cell induction, which is normally seen in wild-type cells, and are only poorly induced to form spores. (biologists.org)

Thirteen years of cell culture using shell-vial assay from clinical specimens from September 1991 to August 2003. (asm.org)
These specimens are from an axenic asynchronous culture of T. brucei brucei, which causes nagana. (sciencephoto.com)

There are several types of bacterial culture methods that are selected based on the agent being cultured and the downstream use. (wikipedia.org)
Improved Methods for the Axenic Culture of Puccinia recondita f. sp. (apsnet.org)

454 pyrosequencing provided 252 Mb of filtered sequence reads from both culture types that were assembled into 10,087 contigs. (biomedsearch.com)

Moreover, skin microbiome studies have heavily relied on 16S rRNA sequencing data in the absence of bacterial culture. (bioportfolio.com)

Incubate the culture at 50-100 µEinsteins/m 2 /s irradiance at 25°C. Maintain under a 14/10h light-dark photoperiod. (atcc.org)

Our goals were to further annotate the A. apis reference genome and to identify genes that are candidates for being differentially expressed during host infection versus axenic culture. (biomedsearch.com)

Concentrations of cells in phytoplankton cultures are generally higher than those found in nature. (fao.org)

The percentage of cells plated (seeded, inoculated) which attach to the surface of the culture vessel within a specified period of time. (sivb.org)
The live food(s) would be cultured in a dedicated vessel. (wetwebmedia.com)

The vaccine, which is administered subcutaneously over an 18-month schedule, is derived from sterile, acellular filtrates of microaerophilic cultures of the avirulent, nonencapsulated V770-NP1-R strain of Bacillus anthracis . (cdc.gov)

In a re-association experiment, cells from the heterotrophic culture were added to an axenic culture of the Anabaena sp. (thefreedictionary.com)
There is hardly a field of biological investigation in which culturing of such cells is not employed. (sivb.org)
Cells, or cultures derived from them, which will grow, survive, or maintain function only when attached to a surface such as glass or plastic. (sivb.org)
The term cell line implies that cultures from it consist of lineages of cells originally present in the primary culture. (sivb.org)

This confusion is especially acute in the field of vertebrate, invertebrate and plant cell culture. (sivb.org)

Mycoplasma contamination is considered to be a potential problem for vaccines that are produced in cell cultures. (cdc.gov)
This term can also be used to describe agents which contaminate cell cultures. (sivb.org)

More particularly, a TR tube is fabricated using a triiodide resin and a method for culturing an axenic culture. (patentsencyclopedia.com)

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Leishmania major parasite stage-dependent host cell invasion and immune evasion. (1/14)

Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota. (2/14)

The genetic toolbox for Leishmania parasites. (3/14)

Epidemiologic consequences of microvariation in Mycobacterium tuberculosis. (4/14)

Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi. (5/14)

Targeting host syntaxin-5 preferentially blocks Leishmania parasitophorous vacuole development in infected cells and limits experimental Leishmania infections. (6/14)

Antileishmanial activity of warifteine: a bisbenzylisoquinoline alkaloid isolated from Cissampelos sympodialis Eichl. (Menispermaceae). (7/14)

The response of dark septate endophytes (DSE) to heavy metals in pure culture. (8/14)