Axenic Culture: Culture of an isolated organism free from any other associating or contaminating organisms.Claviceps: A genus of ascomycetous fungi, family Clavicipitaceae, order Hypocreales, parasitic on various grasses (POACEAE). The sclerotia contain several toxic alkaloids. Claviceps purpurea on rye causes ergotism.Peptones: Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Myxomycetes: A division of organisms that exist vegetatively as complex mobile plasmodia, reproduce by means of spores, and have complex life cycles. They are now classed as protozoa but formerly were considered fungi.Entamoeba histolytica: A species of parasitic protozoa causing ENTAMOEBIASIS and amebic dysentery (DYSENTERY, AMEBIC). Characteristics include a single nucleus containing a small central karyosome and peripheral chromatin that is finely and regularly beaded.Eukaryota: One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Nitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Starvation: Lengthy and continuous deprivation of food. (Stedman, 25th ed)Microalgae: A non-taxonomic term for unicellular microscopic algae which are found in both freshwater and marine environments. Some authors consider DIATOMS; CYANOBACTERIA; HAPTOPHYTA; and DINOFLAGELLATES as part of microalgae, even though they are not algae.Iodides: Inorganic binary compounds of iodine or the I- ion.Photobioreactors: Devices for generating biological products that use light as the energy source. They are used for controlled BIOMASS production such as growing cyanobacteria, mosses, or algae.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Biofuels: Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).Chlorophyta: A phylum of photosynthetic EUKARYOTA bearing double membrane-bound plastids containing chlorophyll a and b. They comprise the classical green algae, and represent over 7000 species that live in a variety of primarily aquatic habitats. Only about ten percent are marine species, most live in freshwater.Nitrate Reductase (NAD(P)H): An iron-sulfur and MOLYBDENUM containing FLAVOPROTEIN that catalyzes the oxidation of nitrite to nitrate. This enzyme can use either NAD or NADP as cofactors. It is a key enzyme that is involved in the first step of nitrate assimilation in PLANTS; FUNGI; and BACTERIA. This enzyme was formerly classified as EC 1.6.6.2.Cladocera: A suborder of CRUSTACEA, order Diplostraca, comprising the water fleas. They are benthic filter feeders that consume PHYTOPLANKTON. The body is laterally compressed and enclosed in a bivalved carapace, from which the head extends.Lysobacter: A genus of gram-negative, rod-shaped, gliding bacteria in the family XANTHOMONADACEAE. Strongly proteolytic, it is involved in lysing a variety of microorganisms.HydrocarbonsChara: A genus of green plants in the family CHARACEAE, phylum STREPTOPHYTA. They have a strong garlic-like odor and are an important food source for waterfowl.Tritrichomonas foetus: A species of flagellate parasitic EUKARYOTE. It possesses a long undulating membrane that is bordered on its outer margin by a flagellum that becomes free posteriorly. This organism causes infections in cows that could lead to temporary infertility or abortion.Tritrichomonas: A genus of flagellate EUKARYOTES possessing three long anterior flagella.Protozoan Infections, Animal: Infections with unicellular organisms formerly members of the subkingdom Protozoa. The infections may be experimental or veterinary.Trichomonas: A genus of parasitic flagellate EUKARYOTES distinguished by the presence of four anterior flagella, an undulating membrane, and a trailing flagellum.Protozoan Infections: Infections with unicellular organisms formerly members of the subkingdom Protozoa.Trichomonadida: An order of parasitic EUKARYOTES typically having four to six flagella. True cysts are known in very few species. Trichomonadida were formerly members of the class Zoomastigophora in the old five kingdom paradigm.Trichomonas vaginalis: A species of TRICHOMONAS that produces a refractory vaginal discharge in females, as well as bladder and urethral infections in males.Commerce: The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)Taxes: Governmental levies on property, inheritance, gifts, etc.Prescription Fees: The charge levied on the consumer for drugs or therapy prescribed under written order of a physician or other health professional.Philately: Study of stamps or postal markings. It usually refers to the design and commemorative aspects of the stamp.Great BritainGermanyMedicine, Arabic: Traditional Arabic methods used in medicine in the ARAB WORLD.Western Australia: A state in western Australia. Its capital is Perth. It was first visited by the Dutch in 1616 but the English took possession in 1791 and permanent colonization began in 1829. It was a penal settlement 1850-1888, became part of the colonial government in 1886, and was granted self government in 1890. (From Webster's New Geographical Dictionary, 1988, p1329)Telefacsimile: A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.Genome, Mitochondrial: The genetic complement of MITOCHONDRIA as represented in their DNA.Sheep: Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.Agricultural Irrigation: The routing of water to open or closed areas where it is used for agricultural purposes.DNA, Mitochondrial: Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.Problem Solving: A learning situation involving more than one alternative from which a selection is made in order to attain a specific goal.Skin Manifestations: Dermatologic disorders attendant upon non-dermatologic disease or injury.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Genetics, Medical: A subdiscipline of human genetics which entails the reliable prediction of certain human disorders as a function of the lineage and/or genetic makeup of an individual or of any two parents or potential parents.Plant Cells: Basic functional unit of plants.Confusion: A mental state characterized by bewilderment, emotional disturbance, lack of clear thinking, and perceptual disorientation.Vocabulary, Controlled: A specified list of terms with a fixed and unalterable meaning, and from which a selection is made when CATALOGING; ABSTRACTING AND INDEXING; or searching BOOKS; JOURNALS AS TOPIC; and other documents. The control is intended to avoid the scattering of related subjects under different headings (SUBJECT HEADINGS). The list may be altered or extended only by the publisher or issuing agency. (From Harrod's Librarians' Glossary, 7th ed, p163)Systematized Nomenclature of Medicine: Controlled vocabulary of clinical terms produced by the International Health Terminology Standards Development Organisation (IHTSDO).Ethical Review: A formal process of examination of patient care or research proposals for conformity with ethical standards. The review is usually conducted by an organized clinical or research ethics committee (CLINICAL ETHICS COMMITTEES or RESEARCH ETHICS COMMITTEES), sometimes by a subset of such a committee, an ad hoc group, or an individual ethicist (ETHICISTS).

Leishmania major parasite stage-dependent host cell invasion and immune evasion. (1/14)

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Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota. (2/14)

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The genetic toolbox for Leishmania parasites. (3/14)

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Epidemiologic consequences of microvariation in Mycobacterium tuberculosis. (4/14)

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Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi. (5/14)

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Targeting host syntaxin-5 preferentially blocks Leishmania parasitophorous vacuole development in infected cells and limits experimental Leishmania infections. (6/14)

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Antileishmanial activity of warifteine: a bisbenzylisoquinoline alkaloid isolated from Cissampelos sympodialis Eichl. (Menispermaceae). (7/14)

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The response of dark septate endophytes (DSE) to heavy metals in pure culture. (8/14)

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D in axenic cultures under conditions of nitrogen starvation , and at least two of these ?SPM1 encoding a serine protease andNutrient Conditions during Rice
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Used directly from the bottle, Supelpak-2SV requires no costly in-house cleaning before use and is superior to other "precleaned" XAD-2 resins, even after additional cleaning.Used directly from the bottle, Supelpak-2SV requires no costly in-house cleaning before use and is superior to other "precleaned" XAD-2 resins, even after additional cleaning.This results in savings in both lab time and hazardous solvent waste generation, disposal and associated costs. Characterisation by GC-MS has also shown Supelpak-2SV resin an excellent choice for semivolatile priority pollutants, PCBs, PAHs and dioxins/ furans. A study of extraction efficiencies using a wide range of semivolatile compounds demonstrated recoveries ,90% even for substituted phenols. Table 2 shows typical recoveries of substituted phenols from Supelpak-2SV compared to competitive products. Customers also appreciate other aspects of the product such as the availability of large single-lot batches of up to 10 kg. This significantly reduces ...
Used directly from the bottle, Supelpak-2SV requires no costly in-house cleaning before use and is superior to other "precleaned" XAD-2 resins, even after additional cleaning.Used directly from the bottle, Supelpak-2SV requires no costly in-house cleaning before use and is superior to other "precleaned" XAD-2 resins, even after additional cleaning.This results in savings in both lab time and hazardous solvent waste generation, disposal and associated costs. Characterisation by GC-MS has also shown Supelpak-2SV resin an excellent choice for semivolatile priority pollutants, PCBs, PAHs and dioxins/ furans. A study of extraction efficiencies using a wide range of semivolatile compounds demonstrated recoveries ,90% even for substituted phenols. Table 2 shows typical recoveries of substituted phenols from Supelpak-2SV compared to competitive products. Customers also appreciate other aspects of the product such as the availability of large single-lot batches of up to 10 kg. This significantly reduces ...
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a From August 1991 to September 2003, using the JNSP shell-vial protocol, we isolated 27 microorganisms for which the standard axenic culture was negative. These bacteria include fastidious, nonfastidious, and noncultivable bacteria. Presented for each microorganism are the results of 16S rRNA PCR, culture, and strain establishment in axenic medium. ...
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0001] The present invention relates to an axenic inoculation system for microalgae using a triiodide resin (TR) tube of a triiodide resin, and a method for culturing an axenic culture. More particularly, a TR tube is fabricated using a triiodide resin and a method for culturing an axenic culture. More particularly, a TR tube is fabricated using a triiodide resin and attached on an upper portion of a photobioreactor (PBR), and used for passing a culture solution containing microalgae, pure liquid and air through the TR tube. The present invention relates to an axenic inoculation system for microalgae using the TR tube which principally prevents contamination with bacteria possibly occurred due to temporary exposure to external environments at the time of inoculation of microalgae during culture, while selectively passing air, the culture and microalgae only through a closed photobioreactor (PBR). Accordingly, the present invention relates a configuration of a selective channel for selectively ...
BioAssay record AID 774779 submitted by ChEMBL: Antiprotozoal activity against axenic amastigote form of Leishmania donovani MHOM/ET/67/L82 assessed as parasite growth inhibition.
Looking for Menispermaceae? Find out information about Menispermaceae. A family of dicotyledonous woody vines in the order Ranunculales distinguished by mostly alternate, simple leaves, unisexual flowers, and a dioecious habit.... Explanation of Menispermaceae
Soil salinity affects large areas of cultivated land, causing significant reductions in crop yield globally. The Na+ toxicity of many crop plants is correlated with overaccumulation of Na+ in the shoot. We have previously suggested that the engineering of Na+ exclusion from the shoot could be achieved through an alteration of plasma membrane Na+ transport processes in the root, if these alterations were cell type specific. Here, it is shown that expression of the Na+ transporter HKT1;1 in the mature root stele of Arabidopsis thaliana decreases Na+ accumulation in the shoot by 37 to 64%. The expression of HKT1;1 specifically in the mature root stele is achieved using an enhancer trap expression system for specific and strong overexpression. The effect in the shoot is caused by the increased influx, mediated by HKT1;1, of Na+ into stelar root cells, which is demonstrated in planta and leads to a reduction of root-to-shoot transfer of Na+. Plants with reduced shoot Na+ also have increased salinity ...
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Ductless bathroom fans remove odors and germs by filtering the air through an activated charcoal filter. These fans have a lower blade speed, also called a tip speed, which allows more air to be...
Air purifier charcoal filter kit . Fits the GE Energy Star 115-Volt air purifier, AFHC09AM (sold separately) . AC filter minimum gram weight of activated charco
Combustion Integration Rack (CIR) Hardware Gather: Virts gathered the equipment for the CIR reconfiguration from FLame Extinguishment Experiment (FLEX)-2 to the FLEX-2J scheduled for Wednesday. Node 1 (N1) and Node 2 (N2) Bacteria/Charcoal Filter Remove & Replace (R&R): Virts removed N1 bacteria filters and replaced them with charcoal filters. Using charcoal filters in Node 1 will reduce the ISS atmospheric Polydimethylsiloxane (PDMS) concentration. Reducing the PDMS atmospheric concentration will subsequently reduce the quantity of dimethylsilanediol (DMSD) produced in the Common Cabin Air Assembly (CCAA) Condensing Heat Exchangers, thereby extending the life of the Multifiltration (MF) Beds that are in a zero spare posture. The bacteria filters that were removed from the N1 will replace the N2 Bacteria filters that were scheduled to be replace per preventative maintenance schedule.. Robotics Refueling Mission (RRM) Hardware Removal: Following the conclusion of last weeks RRM activities, ...
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Abstract. Fast measurements of aerosol and gas-phase constituents coupled with the ISORROPIA-II thermodynamic equilibrium model are used to study the partitioning of semivolatile inorganic species and phase state of Mexico City aerosol sampled at the T1 site during the MILAGRO 2006 campaign. Overall, predicted semivolatile partitioning agrees well with measurements. PM2.5 is insensitive to changes in ammonia but is to acidic semivolatile species. For particle sizes up to 1μm diameter, semi-volatile partitioning requires 15-30 min to equilibrate; longer time is typically required during the night and early morning hours. Aerosol and gas-phase speciation always exhibits substantial temporal variability, so that aerosol composition measurements (bulk or size-resolved) obtained over large integration periods are not reflective of its true state. When the aerosol sulfate-to-nitrate molar ratio is less than unity, predictions improve substantially if the aerosol is assumed to follow the deliquescent ...
Filtered colostomy bags is a subject I have not been able to find any information on. I am looking for a colostomy bag/pouch with a charcoal filter system that actually works. Ive only found one such product on the market but my medical coverage will no longer pay for them; it is the Hollister CenterPointLock Drainable Pouch with replaceable filter. This pouch is a double bag design and it works great when modified by punching additional holes into the inner bag. I am currently
STEMCELL Technologies announced on Friday that it received a $1.5 million grant from the National Research Council of Canada Industrial Research Assistance Program for the development of cell isolation technology.
Direct isolation of untouched immune cells from whole blood, buffy coat, or LRSC in just 25 minutes. Get pure target cells from large volumes without the need for density gradient centrifugation or RBC lysis. - USA
Direct isolation of untouched immune cells from whole blood, buffy coat, or LRSC in just 25 minutes. Get pure target cells from large volumes without the need for density gradient centrifugation or RBC lysis. - Ísland
Cell Biologics provides custom cell isolation service upon request. On request, we will isolate cells of any description from specific organs or tissues (any animal or human tissues) designated by our customers. If you would like to use of Custom Cell Isolation Service, for details regarding these special services, please contact us at [email protected] Thank You!. ...
Cell Biologics provides custom cell isolation service to our customers upon request. On request, we will isolate cells of any description from specific organs or tissues (any animal or human tissues) designated by our customers. If you would like to use of Custom Cell Isolation Service, for details regarding these special services, please contact us at [email protected] Thank You!. ...
Isoliensinine, a natural phenolic bisbenzyltetrahydroisoquinoline alkaloid, has received considerable attention for its potential biological effects such as antioxidant and anti-HIV activities. From the dog hepatic microsomes of isoliensinine, three new N-demethylated and O-demethylated metabolites, 2-N-desmethyl-isoliensinine (M1), 2-N-desmethylisoliensinine (M2), and 2-N-6-O-didesmethylisoliensinine (M3), were identified by high-performance liquid chromatography and data-dependent electrospray ionization tandem mass spectrometry. Possible metabolic pathways for isoliensinine have been proposed. The result should prove very helpful for evaluation of the drug-like properties of isoliensinine and other bisbenzylisoquinoline alkaloids.
AXENIC INOCULATION SYSTEM FOR MICROALGAE USING TR TUBE OF TRIIODIDE RESIN AND METHOD FOR CULTURING AXENIC CULTURE - A triiodide resin (TR) tube of the present invention has a cylindrical shape. A resin exhaust preventing part is provided at a lower portion of the TR tube, and a triiodide resin is received inside the TR tube. A contaminated culture passes through the TR tube to allow an axenic culture to flow out. When a microalgae culture contaminated with bacteria passes through the TR tube filled with the triiodide resin, a pure culture of microalgae can be prepared by using the sterilizing rate of bacteria populations, which is relatively higher than that of microalgae, due to characteristics of iodine exhibiting negative polarity. An axenic inoculation system for microalgae using the TR tube of the present invention comprises the steps of: sterilizing a photobioreactor (PBR) with peracetic acid, washing the PBR with purified water, and filling the PBR with a sterilized culture; allowing a ...
Saprotrophic nutrition /sæprəˈtrɒfɪk, -proʊ-/ or lysotrophic nutrition is a process of chemoheterotrophic extracellular digestion involved in the processing of decayed organic matter. It occurs in saprotrophs and heterotrophs, and is most often associated with fungi (for example Mucor) and soil bacteria. Saprotrophic microscopic fungi are sometimes called saprobes; saprotrophic plants or bacterial flora are called saprophytes (sapro- + -phyte, "rotten material" + "plant"), though it is now believed that all plants previously thought to be saprotrophic are in fact parasites of microscopic fungi or other plants. The process is most often facilitated through the active transport of such materials through endocytosis within the internal mycelium and its constituent hyphae. Various word roots relating to decayed matter (detritus, sapro-), eating and nutrition (-vore, -phage), and plants or life forms (-phyte, -obe) produce various terms, such as detritivore, detritophage, saprotroph, ...
Troupin, G. 1962. Monographie des Menispermaceae africaines. 313 pp. (Mémoires in-8vo, Nouv. sér., Tome XIII, fasc. 2.) Académie Royale des Sciences dOutre-Mer, Classe des Sciences Naturelles et Médicales, Bruxelles. PDF (20 MB) Reference page ...
Author of Umetnost v Primorju, Crngrob, Oris zgodovine umetnosti pri Slovencih, Slikarstvo v Sloveniji od 12. do srede 16. stoletja, Ptujska gora, Monvmenta artis slovenicae
Bisbenzylisoquinoline alkaloids were isolated from Stephania cepharantha Hayata and their proliferative activities on cultured hair cells from the murine skin were evaluated. Cepharanthine (1), cepharanoline (9), isotetrandrine (2), and berbamine (7) showed significant activities in the range of 0.01-0.1 microgram/ml, but had no activity on cultured keratinocytes or fibroblasts from the murine skin.
Ten Leishmania isolates (L. infantum: 6 isolates, L. donovani, L. tropica, L. major, L. aethiopica: one isolate each) have been successfully adapted to grow as axenic amastigote or amastigote-like forms. Promastigote forms were propagated in vitro (27oC, pH 7.2) in a defined liquid medium requiring no serum or serum substitutes at. Amastigotes forms were cultivated in a slightly modified medium [1] substituted with 1% of a commercially available serum fraction produced from abundantly available adult bovine serum (FC III, HyClone) which obviates the need for fetal bovine serum. Axenic amastigotes were produced from promastigotes by increasing the temperature stepwise to 36oC followed by decreasing the pH of the medium stepwise to 5.4 [2 ...
Imagine this: A biodegradable plastic that breaks down instead of sitting in landfills for centuries, activated charcoal filters made from pecan shells, a plant that will pull poisonous mercury from the ground.
Akadeums Mouse CD4+ T Cell Isolation Kit uses microbubbles and a negative selection enrichment method to isolate cells. Read more about the product here.
Taiwan is a continental island lying at the boundary between the Eurasian and the Philippine tectonic plates and possesses high biodiversity. Southern Taiwan, viz. Hengchun Peninsula, is notably floristically different from northern Taiwan. The floristic origin and relationships of the Hengchun Peninsula have been rarely investigated in a phylogenetic context. In this study, data from six plastid and nuclear sequences were used to reconstruct phylogenetic relationships within Burasaieae (Menispermaceae), which mainly inhabits tropical rainforests. The tree-based comparisons indicate that the position of Tinospora sensu stricto conflicts significantly between the cpDNA and ITS trees. However, alternative hypothesis tests from the ITS data did not reject the result of the cpDNA data, which suggests that tree-based comparisons might sometimes generate an artificial incongruence, especially when markers with high homoplasy are used. Based on the combined cpDNA and ITS data, we present an inter ...
PATHA - Botanical Name- Cissampelos parieta Linn. (North India) Cyclea peltata (south India ) . It is one of the best brain tonic used in Ayurveda along with other drugs. In the Vedic literature Pãthã is described as Pata . . usage of patha in Ayurveda with pictures and details.
There are antimicrobial air purifiers, which work to eliminate airborne bacteria and germs. Similar to the HEPA filters, but theres a high powered filtration system with two filters- a pre-filter and a charcoal filter. The air contaminants go into the pre-filter, then pass through the charcoal filter. There the air is zapped with electrostatic light technology and UV sanitizers that kill 99.9% of airborne germs and bacteria. Then the purified air is converted and returned into the room.. The greatest health benefit is for those individuals with chronic breathing conditions that results in compromise lung functioning, this would include Chronic Obstructive Pulmonary Disease (COPD), chronic bronchitis and those suffering from chronic emphysema. This type of air purifier is also very effective during cold and flu season. Because it can literally kill dangerous airborne bacteria before theyre transferred from person-to-person. Click here to know what is the best air purifier for your home.. ...
Systemic ciliatosis caused by histophagous ciliates constitutes a serious disease of cultured turbot. Six ciliate isolates were obtained from parasitized turbot during six epizootics at four different farms located in Spain, France and Portugal. Axenic cultures of the six isolates were obtained by periodical subculturing in ATCC 1651MA or supplemented L-15 media. In basal media or seawater, the parasites could survive starving for long periods with no apparent proliferation. In adequate media, growth kinetics was found to be very similar for isolates A and B, with a clear influence of temperature. Morphological studies demonstrated that all isolates share common features that allows their assignment to either Philasterides Kahl, 1931 or Miamiensis Thompson et Moewus, 1964. However, statistically significant differences were evident in pairwise comparisons of the isolates from the four farm sites in 16 taxonomically relevant morphometric features. This could allow the discrimination of different ...
Some compounds articulated around a piperazine or an ethylenediamine linker have been evaluated in vitro to determine their activity in the presence of a 3T6 fibroblast cell line and an axenic culture of Pneumocystis carinii, respectively. The most efficient antifungal derivatives, namely N,N′-bis(benzamidine-4-yl)ethane-1,2-diamine (compound 6, a diamidine) and N-(benzamidine-4-yl)-N′-phenylethane-1,2-diamine (compound 7, a monoamidine), exhibited no cytotoxicity and were evaluated in vivo in a rat model. Only the diamidine 6 emerged as a promising hit for further studies.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
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... - Instruments Consumables Reagents Advanced BioMatrix,RANDOX,RANDOX ELISA,Biomedical, biochemical reagents, laboratory supplies, equipment, antibodies, ELISA kits, diagnostic reagents, methods of experimental techniques, general analytical instruments, material testing instruments and equipment, used laboratory equipment, instruments and equipment, life sciences, environmental monitoring equipment , measurement, measuring instruments, rotating wall bioreactor, three-dimensional tissue / stem cell culture system; microcapsule
AOAC Official Method AOAC 969.02-1975, Phosphorus (Total) in fertilizers. Alkalimetri - The files are in electronic format(PDF/DOC/DOCX) and will be sent to your email within 24 hours. Test Method:AOAC 969.02-1975Title:Phosphorus (Total) in fertilizers. Alkalimetric quinolinium molybdophosphate methodPages:1
Phenomenex Inc., a global leader in the research and manufacture of advanced technologies for the separation sciences, introduces Zebron™ ZB-SemiVolatiles GC columns. Specially designed for the separation of semivolatile compounds, the new columns feature Phenomenexs proprietary Enviro-Inert™ technology, which improves column inertness for ultra-low-level detection without altering selectivity. Zebron ZB-SemiVolatiles GC columns separate 135 compounds in under 14 minutes, and exceed the
Reto Dorta of the University of Zurich, exploring (J. Am. Chem. Soc. 2010, 132, 15179. ) several alternatives, found that only 4c cyclized cleanly to 5. Karol Grela of the Polish Academy of Sciences showed (Synlett 2010, 2931. ) that 3-nitropropene (not illustrated) participated in cross metathesis when catalyst 1c was used. Shawn K. Collins of the Universit de Montr al complexed (J. Am. Chem. Soc. 2010, 132, 12790. ) 6 with a quinolinium salt to direct paracyclophane formation. Min Shi of the Shanghai Institute of Organic Chemistry incorporated (Org. Lett. 2010, 12, 4462. ) the cyclopropene 8 in cross metathesis, to give 10. A. Srikrishna of the Indian Institute of Science, Bangalore constructed (Synlett 2010, 3015. ) the cyclooctenone 12 by ring-closing metathesis.. ...
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Trypanosoma brucei, i.e. the causative agent of sleeping sickness in humans and of nagana in cattle can undergo apoptosis in the mammalian bloodstream form (BSF) [20, 21] and the procyclic form within the midgut of the tsetse fly [22, 23]. In the mammalian bloodstream, parasitemia of T. brucei increases and decreases periodically and this is partially due to effective antibody-mediated immune responses of the host and antigenic variation of the major surface glycoprotein of the parasite. However, the cell density of T. brucei is also regulated in axenic cultures in the absence of any host-derived immune effectors. After reaching a cell density-dependent threshold, further expansion of the cell population is restricted by differentiation from the replicating long slender (LS) form to the non-dividing short stumpy (SS) form [24]. Subsequently, the parasite density even decreases and this is accompanied by the occurrence of morphological and biochemical markers for apoptosis [20]. Likewise, ...
Algae with potential biotechnological applications in different industries are commonly isolate from the environment in order to obtain pure (axenic) stocks that can be safely stored for long periods of time. To obtain axenic cultures, antibiotics are frequently used, and cryopreservation is applied to keep standing stocks. However, many of these now standard methods were developed using strains coming from pristine-to-low intervened environments and cold-to-temperate regions. Thus, it is still not well understand the potential effect of said methods on the life cycle and biochemical profile of algae isolates from hiper-eutrophic and constant high-temperature tropical regions, which could potentially render them unsuitable for their intended biotechnological application. In this study, we conducted a genetic characterization (18S rRNA) and evaluated the effect of purification (use of the antibiotic chloramphenicol, CPA) and cryopreservation (dimethyl sulfoxide (DMSO)-sucrose mix and Glycerol) on the
We present a comprehensive transcriptome analysis of the fungus Ascosphaera apis, an economically important pathogen of the Western honey bee (Apis mellifera) that causes chalkbrood disease. Our goals were to further annotate the A. apis reference genome and to identify genes that are candidates for being differentially expressed during host infection versus axenic culture. We compared A. apis transcriptome sequence from mycelia grown on liquid or solid media with that dissected from host-infected tissue. 454 pyrosequencing provided 252 Mb of filtered sequence reads from both culture types that were assembled into 10,087 contigs. Transcript contigs, protein sequences from multiple fungal species, and ab initio gene predictions were included as evidence sources in the Maker gene prediction pipeline, resulting in 6,992 consensus gene models. A phylogeny based on 12 of these protein-coding loci further supported the taxonomic placement of Ascosphaera as sister to the core Onygenales. Several common protein
Programmed cell death (PCD) has been observed in many unicellular eukaryotes; however, in very few cases have the pathways been described. Recently the early divergent amitochondrial eukaryote Giardia has been included in this group. In this paper we investigate the processes of PCD in Giardia. We performed a bioinformatics survey of Giardia genomes to identify genes associated with PCD alongside traditional methods for studying apoptosis and autophagy. Analysis of Giardia genomes failed to highlight any genes involved in apoptotic-like PCD; however, we were able to induce apoptotic-like morphological changes in response to oxidative stress (H2O2) and drugs (metronidazole). In addition we did not detect caspase activity in induced cells. Interestingly, we did observe changes resembling autophagy when cells were starved (staining with MDC) and genome analysis revealed some key genes associated with autophagy such as TOR, ATG1 and ATG 16. In organisms such as Trichomonas vaginalis, Entamoeba ...
A filtration and circulation system for maintaining the water quality in a fish tank favorable for promoting the production and growth of fish therein and for circulating water therethrough at a rate designed to provide a high rate of water exchange in the tank. The system includes particulate, biological and activated charcoal filters serially arranged in pressurized and unpressurized sections thereof for converting or removing ammonia, waste food and excrement from the water. In addition, parallel flow paths from these filters through a protein skimmer and an ultraviolet sterilizer section, respectively, are provided for lower volume treatment of the water being circulated therethrough.
2009) The financial benefits of various control strategies against Trypanosoma evansi infection (surra) in backyard livestock in Mindanao, Philippines. In: 76th National Scientific Conference and Annual Convention of the Philippine Veterinary Medical Association (PVMA), 18 - 20 February, Davao City, Philippines. Taylor, T., Boyle, D., Colling, A., Reid, S. and Dargantes, A.P. ...
High-content screening (HCS), also known as high-content analysis (HCA), is a method that is used in drug discovery to identify substances that alter...
Use Human TIMP1 HEK293 Cell Lysate for fast, easy, and consistent DNA/RNA Purification, Antibody/Protein Purification, Cell Isolation.
The card agglutination test for Trypanosoma evansi (CATT/T. evansi) for the detection of antibodies, and Suratex for the detection of circulating antigens were compared in a cross-sectional study involving camels in eastern and central parts of Kenya. Of the 2227 camels screened, 2038 were owned by nomadic pastoralists in T. evansi endemic areas in eastern Kenya. A herd of 86 camels were from a ranch in Mugwoni. In Athi River area, 35 camels belonged to Kenya Trypanosomiasis Research Institute, and 68 were slaughter animals. Diagnostic sensitivity estimates were obtained by testing sera from 51 camels that had been found to be parasitologically positive by the haematocrit centrifugation technique, buffy-coat technique and mouse inoculation. Diagnostic specificity was estimated by testing sera from 35 camels known to be trypanosome-free. Positive and negative predictive values (NPVs) were calculated using a range of prevalence values. The sensitivity of CATT/T. evansi (68.6%) was higher than that ...
Effect of methanolic extract of Cissampelos pareira on Acetic acid and Dextran Sodium Sulphate (DSS) induced inflammatory bowel disease (IBD) in albino rats and mice
Pitting corrosion is characterised by the formation of small anodes and corresponding large cathodes, which allow the formation of pits in the metal matrix. A number of mechanisms have been put forward to account for the involvement of SRB in this process including: cathodic depolarization of hydrogen, bacterial sulphide acting as the cathode, and changes in pH due to sulphate-reducing bacteria (SRB). To date there has been little success in reproducibly inducing SRB pitting of stainless steels in a laboratory system. A concentric electrode has been designed in which a small anode is artificially produced within a large cathode by applying a galvanic current of 12 muA /sq cm. During the application of this galvanic current, various consortia and axenic cultures of bacteria are allowed to colonise the surface of the stainless steel concentric electrode. After seventy-two hours the induced current is removed and the resultant current monitored. Electrochemical impedance spectroscopy (EIS) is carried out
Effective for radioactive water. Clay binds to radioactive particles. If radioactive fallout has contaminated the water supplies, earth filters utilizing clay type soil, will effectively remove the radioactive particles from the water. This method is better than distillation, ion-exchange filters, or charcoal filters for this purpose. Perforate the bottom of a 5-gallon can or wastebasket with holes punched within 2 inches of the center. Place a two-inch layer of washed pebbles on the bottom of the can. Cover the pebbles with one thickness of terry cloth towel or other porous cloth. Scrape the top 4-5 inches of soil off the ground to get below the fallout, and dig enough clay-type soil to fill the can to a depth of 8 inches, packing it tightly against the sides. Cover the soil in the can with another thickness of toweling and another one or two inches of pebbles. Suspend the can over a clean container and pour the contaminated water into the top. Clear (but unpurified) water will come out the ...
But you can have degrees of filtration for different purposes. My parents have a well and have a whole-house sediment filter (cheap) where the line runs into the house, which strips out the worst of the stuff and keeps it from eating the pipes, and then run drinking water through an under-the-counter activated charcoal filter in the kitchen for drinking and making coffee. They just decided that removing the sulfur from bathing water wasnt worth the cost. Your friends should consider their options and weigh them against how much they care. You can buy a lot of replacement faucets and toilet flapper valves for the price of a whole-house RO system or even a good high-volume filter ...
My pack space for my Thursday trip is at a severe premium, so if I can leave behind my filter kit, that would really help. Chlorine dioxide (Aquamira) is effective against cysts, but it is relatively expensive, plus its not available in stores in my town. If I were to pick some up on the way it would add about an hour to my trip. If I order online, I have to pay shipping, and it might not get here on time. The local WM does have an iodine based treatment (yuck), but I was thinking maybe bleach would fit the bill. Ive read the US recommendations for sanitizing drinking water with bleach, and Im comfortable with it. I also have a couple of in-bottle charcoal filters to help with the taste. However, bleach is not effective against giardia and cryptosporidium. Does anyone know if these are an issue in the Caney Creek Wilderness ...
Trimethoprim ………………… 160 mg. Fish-Sulfa Forte™ (Sulfamethoxazole/Trimethoprim or SMZ-TMP) is an antibiotic that exerts a bactericidal action on gram-positive and some gram negative bacteria in fish. It is useful for control of some common bacterial diseases in fish, including diseases caused by bacteria such as Areomonas, Pseudomonas, and Mycobacterial (Gill deseases and Chondrococcus).. Directions: Add one tablet (960 mg) into aquarium for each 40 gallons of water to be treated. Repeat in 24 hours. It is suggested that a partial water change be made between treatments. While duration of treatment depends on type and severity of infection, it is recommended that extended medication baths continue for a minimum of 5 days but not more than 10 days. Discontinue treatment with this medication if no improvement is noted within 5 days. To remove harmless yellow color, change 20% of water and use charcoal filter until clear.. Warnings: For aquarium and ornamental fish only. Not for ...
Brotowali (Tinospora crispa (L.) Miers.hen jin t) Sinonim : Tinospora rumphii, Boerl. T. tuberculata Beumee. Cocculus crispus, DC. Menispermum verrucosum. M.crispum, Linn. M.tuberculatum, Lamk. Familia : Menispermaceae Uraian : Tumbuhan liar di hutan, ladang atau ditanam dihalaman dekat pagar. Biasa ditanam sebagai tumbuhan obat. Menyukai tempat panas, termasuk perdu, memanjat, tinggi batang sampai 2,5 m.…
The PerkinElmer Elite 608 columns specifically designed for the analysis of semivolatile pesticides by US EPA method 608. The mid polarity phase is a phenyl methyl polysiloxane phase.
Use Human IL1R1(Fc) HEK293 Cell Lysate for fast, easy, and consistent DNA/RNA Purification, Antibody/Protein Purification, Cell Isolation.
Contaldo, N.; Bertaccini, A.; Paltrinieri, S.; Windsor, H.M.; Windsor, G.D. (2012). "Axenic culture of a plant pathogenic ... Tissue culture can be used to produce healthy clones of phytoplasma-infected plants. Cryotherapy (i.e., the freezing of plant ... status (used for bacteria that cannot be cultured). Phytoplasma taxonomy is complicated because the organisms cannot be ... and cannot be cultured. Viral and phytoplasmic infections share some symptoms. In 1967, phytoplasmas were discovered in ...
Sokolski, K.; Dovholuk, A.; Dovholuk, L.; Faletra, P. (1997). "Axenic seed culture and micropropagation of Cypripedium reginae ...
Gametophytes of Lycopodium obscurum as grown in axenic culture. Canadian Journal of Botany. 55. pp. 563-567. Hickey, R. James. ...
Specific-pathogen-free (SPF) Axenic culture Reyniers JA (1959). "Germfree Vertebrates: Present Status". Annals of the New York ...
Berch SM, Castellano MA (1986). "Sporulation of Endogone pisiformis in axenic and monaxenic culture". Mycologia. 78 (2): 292- ... 1990). "Taxonomy, culture, and mycorrhizal associations of some zygosporic Endogonaceae". Mycological Research. 94 (2): 173-178 ... pisiformis can be made to sporulate in test tube culture when grown with conifer seedlings. Endogone was first circumscribed by ...
The mycelium of Tylopilus felleus can be grown in axenic culture, on agar containing growth medium. The fungus can form fruit ... 1974). "Fruitbody formation by Tylopilus fellus in axenic culture". Mycologia. 66 (1): 197-202. doi:10.2307/3758474. JSTOR ... There are few Boletaceae species known to fruit in culture, as ectomycorrhizal fungi tend to not fruit when separated from ...
Baker, KL; Beneke, ES; Hooper, GR; Fields, WG (1977). "Host Range and Axenic Culture of the Mycoparasite Syncephalis sphaerica ...
This work was important in the development of axenic culture methods. C. briggsae can often be found in compost, garden beds, ...
The tips of the hyphae were used to obtain axenic cultures; the fungus can grow on a variety of media commonly used to grow ... Escobar grew cultures of the fungus by placing fresh fruit bodies on agar containing growth medium with an extract of horse ... Experimental observations on the development of the fruit body, based on the growth on the fungus in pure culture, suggest that ... Mycologist Dennis McCabe studied the development of the fruit body using specimens grown in pure culture. Starting out as a ...
I. Isoenzyme and Soluble Protein Patterns of Axenic Cultures of Symbiodinium microadriaticum". Proceedings of the Royal Society ... In culture, the discarded walls accumulate at the bottom of the culture vessel. It is not known what becomes of the walls from ... All cultured isolates (i.e. strains) are capable of phenotypic adjustment in their capacity for light harvesting (i.e. ... As cultures reach stationary growth phase, fewer and fewer motile cells are observed, indicating slower division rates. Large ...
It was difficult to raise from seed, taking many months to germinate in sterile culture until progress on axenic culture from ... 1997). "Axenic Seed Culture and Micropropagation of Cypripedium reginae" Selbyana 18(2): 172-182. Correll, Donovan (1978) [1950 ... in Axenic Seed Culture, Bulletin of America Association for the Advancement of Sciences, Annual meeting, pp. A-112 AAAS Annual ... in Axenic Seed Culture. Walsh, V.; N. Ramos; P. Faletra (2013). "Characterization of Cypripedium species and Habitats in New ...
These cultures have strain numbers preceded by BEA. Non-axenic cultures are marked with "B" at the end of the strain number. ... BEA has been member of the European Culture Collection Organization (ECCO) since 2001 and of the World Federation for Culture ... "Spanish Type Culture Collection (University of Valencia)" (in Spanish). Retrieved 26 December 2011. "European Culture ... gDNA "à la carte": BEA is able to extract genomic DNA from microalgae cultures from a wide range of volumes. Normally kits (i.e ...
"Metabolites from the Fungal Endophyte Aspergillus austroafricanus in Axenic Culture and in Fungal-Bacterial Mixed Cultures". ...
Production of DMS from dissolved DMSP in axenic cultures of the marine phytoplankton species Phaeocystis sp., Mar. Ecol. Prog. ...
Prokaryotes where no pure (axenic) cultures are isolated or available; i.e., they are not cultivated or cannot be sustained in ...
... multiseries cultures which were completely axenic (bacteria-free), produce less domoic acid than P. multiseries cultures which ... "Enhancement of domoic acid production by reintroducing bacteria to axenic cultures of the diatom Pseudo-nitzschia multiseries ... For example, in a mixed batch culture the target species may die or have reduced growth rates due to competition for nutrients ... All methods of detecting phycotoxins involve extraction of the candidate toxin from a phytoplankton culture; therefore, it's ...
"Effect of ammonium ions on egg hatching and second-stage juveniles of Meloidogyne incognita in axenic tomato root culture". ...
It was first isolated in axenic culture by J.W. Vinson in 1960, from a patient in Mexico City. He then followed Koch's ... Release of an icosahedral particle, 40 nm in length, has been detected in cultures of B. quintana's close relative, B. henselae ... To have a definite diagnosis of infection with B. quintana requires either serological cultures or nucleic acid amplification ...
An axenic culture of TM7 from the oral cavity was reported in 2014 but no sequence or culture was made available. Along with ... "Axenic Culture of a Candidate Division TM7 Bacterium from the Human Oral Cavity and Biofilm Interactions with Other Oral ... a Recently Recognized Major Lineage of the Domain Bacteria with No Known Pure-Culture Representatives". Applied and ...
The culture is kept under lighting with temperature and pH value held constant. The culture medium-often a minimal medium- ... Algae bioreactor Bioreactor Molecular farming Photobioreactor Physcomitrella patens Hohe, A.; Reski, R. (2005). "From axenic ... "Production of a heterologous protein in bioreactor cultures of fully differentiated moss plants" (PDF). Plant Tissue Culture ... At this point the culture has to be diluted with fresh medium if it is intended for further use. According to the intended ...
... of the early laboratory work on Caenorhabditis nematodes was directed towards the establishment of a defined axenic culture ... Ellsworth C. Dougherty; Eder Lindsay Hansen; Warwick L. Nicholas; J. Anthony Mollett; Evangeline A. Yarwood (1959). "Axenic ... from a laboratory culture of the closely related nematode Caenorhabditis briggsae. However much ...
Amino acid uptake and metabolism by larvae of the marine worm Urechis caupo (Echiura), a new species in axenic culture. ...
Axenic culture University of Michigan Germ Free Animal Facility - example facility for raising germ-free animals Reyniers, J.A ...
It is often essential to isolate a pure culture of microorganisms. A pure (or axenic) culture is a population of cells or ... Stab cultures are most commonly used for short-term storage or shipment of cultures. Microbial culture collections focus on the ... Blood culture International Collection of Microorganisms from Plants Microbial Food Cultures NCPPB Screening cultures Sputum ... the isolation of pure cultures uses the same techniques as for bacterial cultures. Pure cultures of multicellular organisms are ...
V. chlorellavorus will not grow in axenic cultures, depending on access to living cells of its preferred algae host, Chlorella ... Since Vampirovibrio chlorellavorus has not been cultured in recent years, it is possible to learn about its future research ... Subsequently, Soo and Hugenholtz's team performed a genomic reconstruction in 2014 from a culture previously deposited into the ... chlorellavorus in a lysis experiment with the algae Chlorella vulgaris from Ukrainian reservoir waters from a mass culture of ...
... of the host and grow in axenic culture, at least some species may be facultative symbionts. The "amoeb-" prefix refers to the ...
However, in 2009 scientists reported a technique allowing the Q-fever pathogen Coxiella burnetii to grow in an axenic culture ...
Prokaryotes where no pure (axenic) cultures are isolated or available, i.e., not cultivated or cannot be sustained in culture ... "The first 1000 cultured species of the human gastrointestinal microbiota". FEMS Microbiology Reviews. 38 (5): 996-1047. doi ...
... thereby obtaining an axenic culture; and inoculating the axenic culture into a culture liquid inside the PBR to culture ... inoculate the axenic culture in a culture solution inside the PBR to culture microalgae. 10. The axenic inoculation system ... installed at an upper portion of the PBR to obtain an axenic culture, and then, inoculating the axenic culture in a culture ... installed at the upper portion of the PBR to obtain an axenic culture; and inoculate the axenic culture in a culture solution ...
Culture System: Axenic. Subcultivation Protocol: at pH 7.0. Cryopreservation Harvest and Preservation *Harvest cells from a ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just ... Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal ...
Culture Strains. Trophozoites from the E. histolytica HM1: IMSS strain were cultured in TYI-S-33 medium under axenic conditions ... "CLUPS": A New Culture Medium for the Axenic Growth of Entamoeba histolytica. F. Gonzalez-Salazar,1,2 I. Meester,2 F. J. Guzmán ... We introduce a new culture medium (CLUPS) for the axenic growth of E. histolytica. CLUPS medium is a modification of the PEHPS ... Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, ...
Find out information about axenic culture. The growth of organisms of a single species in the absence of cells or living ... organisms of any other species Explanation of axenic culture ... Related to axenic culture: pure culture, monoxenic culture. ... Axenic culture , Article about axenic culture by The Free Dictionary https://encyclopedia2.thefreedictionary.com/axenic+culture ... In a re-association experiment, cells from the heterotrophic culture were added to an axenic culture of the Anabaena sp.. ...
Of the three cultures, Ochrobactrum showed most metabolic activity on the widest range of NA surrogates. Brevundomonas and ... These pure cultures were screened for their ability to use a range of aliphatic, cyclic and aromatic NA surrogates in 96-well ... Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor ...
The Nematode C. elegans as an Animal Model to Explore Toxicology In Vivo: Solid and Axenic Growth Culture Conditions and ... Axenic culture of free‐living, parasitic and insect‐parasitic nematodes. Annu. Rev. Phytopathol. 16:131‐157. ... This unit includes protocols for culturing worms on solid and axenic media and acute and chronic exposure parameters for ... Use of CeHR Axenic Medium for Exposure and Gene Expression Studies. Reproductive Hazards Laboratory, U.S. Army Center for ...
Methods A wide range of Cd and Zn concentrations were applied to ectomycorrhizal fungi in axenic cultures (in mg L−1): 0; 1; 3 ... Cd and Zn interactions and toxicity in ectomycorrhizal basidiomycetes in axenic culture. Vinicius H. De Oliveira, Mark Tibbett​ ... A wide range of Cd and Zn concentrations were applied to ectomycorrhizal fungi in axenic cultures (in mg L−1): 0; 1; 3; 9; 27; ... De Oliveira VH, Tibbett M. (2018) Cd and Zn interactions and toxicity in ectomycorrhizal basidiomycetes in axenic culture. ...
Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are ... Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are ... Sand Cultures Under Axenic Conditions. Rooted poplars can also be grown in sand as the supporting medium under axenic ... Cocultivation Under Axenic Conditions. Culture systems for the cultivation of poplars with L. bicolor or P. involutus under in ...
The work presented herein focused on determining axenic conditions for culturing and growing insect (epimastigote) forms of T. ... Axenic culture Is the Subject Area "Axenic culture" applicable to this article? Yes. No. ... Establishment of T. vivax axenic cultures and impact of serum source. We started T. vivax adaptation to axenic cultures using ... Parasite maintenance in axenic cultures and in vitro differentiation. Epimastigote cultures were initiated with the blood of ...
D in axenic cultures under conditions of nitrogen starvation , and at least two of these ?SPM1 encoding a serine protease ... D in axenic cultures under conditions of nitrogen starvation [13,14], and at. Posted on July 24, 2017. by Proteasome inhibitor ... D in axenic cultures under conditions of nitrogen starvation [13,14], and at least two of these ?SPM1 encoding a serine ... Moreover, the up.D in axenic cultures under conditions of nitrogen starvation [13,14], and at least two of these ?SPM1 encoding ...
Application of genomics, transcriptomics, proteomics and metabolomics to their study in pure/mixed cultures and environmental ... with further novel taxonomic groupings known to exist based on culture-independent molecular surveys. However, the true extent ... with further novel taxonomic groupings known to exist based on culture-independent molecular surveys. However, the true extent ... Changes in fermentation end products of anaerobic fungal metabolism in axenic culture compared to co-culture with methanogens, ...
Axenic Isolates. Fungi and bacteria-free plates were transferred to axenic culture by placing the amoebae in PYG medium as ... DNA from amoebic cultures was extracted by placing 1-2 mL of axenic Acanthamoeba cultures directly into the Maxwell 16 Tissue ... Axenic Cultures. Monoxenic cultures were selected from areas of profuse amoebic growth. Selected pieces of agar were ... Briefly, axenic cultures of A. griffini trophozoites in the exponential phase of growth (72 h) were chilled at 4°C and ...
Culture System: Axenic. Cryopreservation Harvest and Preservation *Harvest cells from a culture that is at or near peak density ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just ... Incubate the culture at 50-100 µEinsteins/m2/s irradiance at 25°C. Maintain under a 14/10h light-dark photoperiod. ...
Culture System: Axenic. Cryopreservation Harvest and Preservation *To achieve the best results, set up cultures with several ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... Harvest cultures and pool when the culture that received the lowest inoculum is at or near peak density. ... To establish a culture from the frozen state, place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just ...
Growth and Terpenoid Production of an Axenic Culture from the Liverwort Ricciocarpos natans. Wurzel, Gerald / Becker, Hans ...
Aquatic plants stimulate the growth of and biofilm formation by Mycobacterium ulcerans in axenic culture and harbor these ...
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PCR before sample culture. Axenic culture. Cell culture detection at day 21. ... Characteristics of 27 microorganisms only isolated in the shell-vial culture by the JNSP protocol for which axenic cultures ... Presented for each microorganism are the results of 16S rRNA PCR, culture, and strain establishment in axenic medium. ... of positive shell-vial cultures. Species. No. of strains established from shell-vial culture. ...
The phytoplankton cultures examined were, Prochlorococcus (axenic and xenic), Synechococcus (axenic), Emiliania huxleyi, ... All cultures were axenic except C. leptoporous. The cultures were grown at 20-25 °C (depending on species) in an even split ... All cultures were axenic, with the exception of Porphyridium sp. and Prorocentrum sp. Cultures were bubbled with ultra-high ... It was unclear whether the cultures were axenic. Scarratt and Moore [92] examined cultures of several marine phytoplanktons: ...
Axenic cultures of symbiotic algae. *Modulated fluorescence of chlorophyll, kinetics (fluorometer, actinic light) ... Microbial cultures. Development of bioreactors under thermal insulation; genetic characterization and enzymatic analysis of ...
The earliest axenic cultures were of bacteria or unicellular eukaryotes, but axenic cultures of many multicellular organisms ... identifying cultures that are no longer axenic. As axenic cultures are derived from very few organisms, or even a single ... Axenic culture is also an important tool for the study of symbiotic and parasitic organisms in a controlled manner. Axenic ... Axenic cultures are usually checked routinely to ensure that they remain axenic. One standard approach with microorganisms is ...
Axenic Culture. Culture of an isolated organism free from any other associating or contaminating organisms. ... A shift from culture-dependent to culture-independent ide.... Metagenomic analyses of the gut microbiota associated with ... Culture. A collective expression for all behavior patterns acquired and socially transmitted through symbols. Culture includes ... Myriads of elements affect human embryo culture in vitro. These include, but not limited to, air quality, culture media, ...
Use of Axenic Culture Tools to Study Coxiella burnetii.. Authors:. Savannah E Sanchez Eduardo Vallejo-Esquerra Anders Omsland ... Lipid A Has Significance for Optimal Growth of in Macrophage-Like THP-1 Cells and to a Lesser Extent in Axenic Media and Non- ... Here we report the characterization of the role of lipid A in growth in axenic media, monkey kidney cells (BGMK and Vero), and ... Molecular detection of Coxiella burnetii in heart valve tissue from patients with culture-negative infective endocarditis.. ...
Pegler in axenic culture and obtained successful basidiomata production.. Production of Panus strigellus spawn using the ... Development in vitro of the symbiotic fungus of Atta sexdens rubropilosa Forel, 1908 (Hymenoptera: Formicidae) in culture ...
Cultures of unicellular cyanobacterial isolates were axenic.. Heterotrophic bacteria used as test strains were isolated from ... Pure cultures were maintained at room temperature on marine agar slants, while cyanobacterial cultures were maintained on ... All filamentous cyanobacteria were cultured from individual trichomes and were uni-algal cultures. Accompanying bacteria were ... were obtained as non-axenic uni-algal cultures as previously described [33,37]. The three BBD cyanobacterial genera have been ...
  • Axenic cultivation of E. histolytica was first accomplished by Diamond in 1961 [ 9 ], using a diphasic medium composed of a serum-enriched agar base covered by a broth supplemented with chicken extract and vitamins. (hindawi.com)
  • In obtaining a culture from another laboratory, the proper designation of the culture, as originally named and described, must be maintained and any deviations in cultivation from the original must be reported in any publication. (sivb.org)
  • They are digenetic parasites: the flagellated promastigote forms, which can be derived in axenic culture, differentiate within the alimentary tracts of sandfly vectors from a replicating procyclic to a nonreplicating, infectious metacyclic stage, whereas obligately intracellular amastigotes live and replicate in mammalian mononuclear phagocytes, such as macrophages ( 1 ). (asm.org)
  • We further demonstrated that reduction of CK1 activity by specific inhibitors, such as D4476, blocks promastigote growth, strongly compromises axenic amastigote viability, and decreases the number of intracellular Leishmania donovani and L. amazonensis amastigotes in infected macrophages. (asm.org)
  • A morphological description must await achieving axenic culture , but the partial sequence of the SSUrDNA has been given the GenBank accession numbers AF228362 and AF228363 for the V1, and V2 and V3 regions of the SSUrDNA, respectively. (thefreedictionary.com)
  • Cultures are authenticated using morphological and molecular techniques. (atcc.org)
  • The work presented herein focused on determining axenic conditions for culturing and growing insect (epimastigote) forms of T. vivax and prompting their differentiation into metacyclic forms that are infectious for the mammalian host. (plos.org)
  • Although culturing plant tissues and organs under axenic conditions was first established and profitably employed in bryophytes, especially mosses [ 13 ], bryophytes did not retain for long their rightful place as a highly favored research object. (biomedcentral.com)
  • Microbial cultures. (uv.es)
  • Although these procedures are used to prevent microbial contamination of cultures, they also prevent cross contamination of cell cultures as well. (sivb.org)
  • Microbial cultures are foundational and basic diagnostic methods used extensively as a research tool in molecular biology . (wikipedia.org)
  • Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both. (wikipedia.org)
  • Anaerobic agents are the most difficult to detect from oxygen-containing environments and, therefore, it is critical that anaerobic collection culture media, anaerobic chambers, and powerful microbial identification technologies be utilized to ensure accurate results. (criver.com)
  • To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. (atcc.org)
  • To establish a culture from the frozen state, place an ampule in a water bath set at 35°C (2-3 min). (atcc.org)
  • A completely axenic C. burnetii genetic transformation system was developed using ACCM-2 that allowed isolation of transformants in about 2 1/2 weeks. (asm.org)
  • Culture media are undefined because they contain mammalian serum, which is a rich source of complex lipids and proteins. (figshare.com)
  • The recent description of a method for axenic (host cell-free) propagation of C. burnetii is a significant technological advance that will facilitate biochemical and genetic studies of the pathogen ( 22 ). (asm.org)
  • A pure culture may originate from a single cell or single organism, in which case the cells are genetic clones of one another. (wikipedia.org)
  • This unit includes protocols for culturing worms on solid and axenic media and acute and chronic exposure parameters for Parkinson's disease-associated toxins and hemin chloride. (currentprotocols.com)
  • Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. (hindawi.com)
  • Various specific recipes for algal culture media are described by Vonshak (1986). (fao.org)
  • The complexity and cost of the above culture media often excludes their use for large-scale culture operations. (fao.org)
  • Diagnosis is based on serologic analysis, culture, and molecular biology. (cdc.gov)
  • Such diseases were originally thought to be caused by viruses, which, like phytoplasmas, require insect vectors, and cannot be cultured. (wikipedia.org)
  • they were cultured without flagellates in the first phase of the experiments and in the presence of the flagellates in the second phase. (asm.org)
  • In monolayer culture experiments, DH1-derived gskA -null cells do not display the cAMP repression of stalk cell induction, which is normally seen in wild-type cells, and are only poorly induced to form spores. (biologists.org)
  • 454 pyrosequencing provided 252 Mb of filtered sequence reads from both culture types that were assembled into 10,087 contigs. (biomedsearch.com)
  • Incubate the culture at 50-100 µEinsteins/m 2 /s irradiance at 25°C. Maintain under a 14/10h light-dark photoperiod. (atcc.org)
  • Our goals were to further annotate the A. apis reference genome and to identify genes that are candidates for being differentially expressed during host infection versus axenic culture. (biomedsearch.com)
  • Concentrations of cells in phytoplankton cultures are generally higher than those found in nature. (fao.org)
  • The percentage of cells plated (seeded, inoculated) which attach to the surface of the culture vessel within a specified period of time. (sivb.org)
  • The live food(s) would be cultured in a dedicated vessel. (wetwebmedia.com)
  • The vaccine, which is administered subcutaneously over an 18-month schedule, is derived from sterile, acellular filtrates of microaerophilic cultures of the avirulent, nonencapsulated V770-NP1-R strain of Bacillus anthracis . (cdc.gov)
  • In a re-association experiment, cells from the heterotrophic culture were added to an axenic culture of the Anabaena sp. (thefreedictionary.com)
  • There is hardly a field of biological investigation in which culturing of such cells is not employed. (sivb.org)
  • Cells, or cultures derived from them, which will grow, survive, or maintain function only when attached to a surface such as glass or plastic. (sivb.org)
  • The term cell line implies that cultures from it consist of lineages of cells originally present in the primary culture. (sivb.org)
  • Mycoplasma contamination is considered to be a potential problem for vaccines that are produced in cell cultures. (cdc.gov)
  • This term can also be used to describe agents which contaminate cell cultures. (sivb.org)