Axenic culture. Medical search

Culture of an isolated organism free from any other associating or contaminating organisms.

A genus of ascomycetous fungi, family Clavicipitaceae, order Hypocreales, parasitic on various grasses (POACEAE). The sclerotia contain several toxic alkaloids. Claviceps purpurea on rye causes ergotism.

Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)

Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.

A division of organisms that exist vegetatively as complex mobile plasmodia, reproduce by means of spores, and have complex life cycles. They are now classed as protozoa but formerly were considered fungi.

A species of parasitic protozoa causing ENTAMOEBIASIS and amebic dysentery (DYSENTERY, AMEBIC). Characteristics include a single nucleus containing a small central karyosome and peripheral chromatin that is finely and regularly beaded.

One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.

Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.

The relationships of groups of organisms as reflected by their genetic makeup.

DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.

An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.

Lengthy and continuous deprivation of food. (Stedman, 25th ed)

A non-taxonomic term for unicellular microscopic algae which are found in both freshwater and marine environments. Some authors consider DIATOMS; CYANOBACTERIA; HAPTOPHYTA; and DINOFLAGELLATES as part of microalgae, even though they are not algae.

Inorganic binary compounds of iodine or the I- ion.

Devices for generating biological products that use light as the energy source. They are used for controlled BIOMASS production such as growing cyanobacteria, mosses, or algae.

Exclusive legal rights or privileges applied to inventions, plants, etc.

Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).

A phylum of photosynthetic EUKARYOTA bearing double membrane-bound plastids containing chlorophyll a and b. They comprise the classical green algae, and represent over 7000 species that live in a variety of primarily aquatic habitats. Only about ten percent are marine species, most live in freshwater.

An iron-sulfur and MOLYBDENUM containing FLAVOPROTEIN that catalyzes the oxidation of nitrite to nitrate. This enzyme can use either NAD or NADP as cofactors. It is a key enzyme that is involved in the first step of nitrate assimilation in PLANTS; FUNGI; and BACTERIA. This enzyme was formerly classified as EC 1.6.6.2.

A suborder of CRUSTACEA, order Diplostraca, comprising the water fleas. They are benthic filter feeders that consume PHYTOPLANKTON. The body is laterally compressed and enclosed in a bivalved carapace, from which the head extends.

A genus of gram-negative, rod-shaped, gliding bacteria in the family XANTHOMONADACEAE. Strongly proteolytic, it is involved in lysing a variety of microorganisms.

A genus of green plants in the family CHARACEAE, phylum STREPTOPHYTA. They have a strong garlic-like odor and are an important food source for waterfowl.

A species of flagellate parasitic EUKARYOTE. It possesses a long undulating membrane that is bordered on its outer margin by a flagellum that becomes free posteriorly. This organism causes infections in cows that could lead to temporary infertility or abortion.

A genus of flagellate EUKARYOTES possessing three long anterior flagella.

Infections with unicellular organisms formerly members of the subkingdom Protozoa. The infections may be experimental or veterinary.

A genus of parasitic flagellate EUKARYOTES distinguished by the presence of four anterior flagella, an undulating membrane, and a trailing flagellum.

Infections with unicellular organisms formerly members of the subkingdom Protozoa.

An order of parasitic EUKARYOTES typically having four to six flagella. True cysts are known in very few species. Trichomonadida were formerly members of the class Zoomastigophora in the old five kingdom paradigm.

A species of TRICHOMONAS that produces a refractory vaginal discharge in females, as well as bladder and urethral infections in males.

The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)

Governmental levies on property, inheritance, gifts, etc.

The charge levied on the consumer for drugs or therapy prescribed under written order of a physician or other health professional.

Study of stamps or postal markings. It usually refers to the design and commemorative aspects of the stamp.

Traditional Arabic methods used in medicine in the ARAB WORLD.

A state in western Australia. Its capital is Perth. It was first visited by the Dutch in 1616 but the English took possession in 1791 and permanent colonization began in 1829. It was a penal settlement 1850-1888, became part of the colonial government in 1886, and was granted self government in 1890. (From Webster's New Geographical Dictionary, 1988, p1329)

A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.

The genetic complement of MITOCHONDRIA as represented in their DNA.

Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.

The routing of water to open or closed areas where it is used for agricultural purposes.

Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.

A learning situation involving more than one alternative from which a selection is made in order to attain a specific goal.

Dermatologic disorders attendant upon non-dermatologic disease or injury.

One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

Leishmania major parasite stage-dependent host cell invasion and immune evasion. (1/14)

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Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota. (2/14)

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The genetic toolbox for Leishmania parasites. (3/14)

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Epidemiologic consequences of microvariation in Mycobacterium tuberculosis. (4/14)

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Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi. (5/14)

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Targeting host syntaxin-5 preferentially blocks Leishmania parasitophorous vacuole development in infected cells and limits experimental Leishmania infections. (6/14)

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Antileishmanial activity of warifteine: a bisbenzylisoquinoline alkaloid isolated from Cissampelos sympodialis Eichl. (Menispermaceae). (7/14)

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The response of dark septate endophytes (DSE) to heavy metals in pure culture. (8/14)

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Berch SM, Castellano MA (1986). "Sporulation of Endogone pisiformis in axenic and monaxenic culture". Mycologia. 78 (2): 292- ... 1990). "Taxonomy, culture, and mycorrhizal associations of some zygosporic Endogonaceae". Mycological Research. 94 (2): 173-178 ... pisiformis can be made to sporulate in test tube culture when grown with conifer seedlings. Endogone was first circumscribed by ...

https://en.wikipedia.org/wiki/Endogone

Baker, KL; Beneke, ES; Hooper, GR; Fields, WG (1977). "Host Range and Axenic Culture of the Mycoparasite Syncephalis sphaerica ...

https://en.wikipedia.org/wiki/Rhizopus_stolonifer

I. Isoenzyme and Soluble Protein Patterns of Axenic Cultures of Symbiodinium microadriaticum". Proceedings of the Royal Society ... In culture, the discarded walls accumulate at the bottom of the culture vessel. It is not known what becomes of the walls from ... Culturing is a selective process, and many Symbiodinium isolates growing on artificial media are not typical of the species ... All cultured isolates (i.e. strains) are capable of phenotypic adjustment in their capacity for light harvesting (i.e. ...

https://en.wikipedia.org/wiki/Symbiodinium

"Effect of ammonium ions on egg hatching and second-stage juveniles of Meloidogyne incognita in axenic tomato root culture". ...

https://en.wikipedia.org/wiki/Root-knot_nematode

Production of DMS from dissolved DMSP in axenic cultures of the marine phytoplankton species Phaeocystis sp., Mar. Ecol. Prog. ... not in culture), P. cordata, and P. rex. Three species (P. globosa, P. pouchetii, and P. antarctica) are associated with bloom ...

https://en.wikipedia.org/wiki/Phaeocystis

... they were able to grow the organism in axenic cultures from single-spore isolates of C. geaster. Until the one fungus, one name ... Finally, only Japanese specimens can be grown in culture-the spores of Texan material have not been successfully germinated on ...

https://en.wikipedia.org/wiki/Chorioactis

... of the host and grow in axenic culture, at least some species may be facultative symbionts. The "amoeb-" prefix refers to the ...

https://en.wikipedia.org/wiki/Amoebidiidae

He grew over 150 trichomycete isolates in axenic culture, a critical step for experimental studies on nutritional modes and ...

https://en.wikipedia.org/wiki/Robert_W._Lichtwardt

However, in 2009, scientists reported a technique allowing the bacteria to grow in an axenic culture and suggested the ...

https://en.wikipedia.org/wiki/Coxiella_burnetii

... penetrates the rhizoids of liverworts and may form a type of symbiosis with them, but in axenic culture tests, L. ... Bresinsky, A. & Schötz, A. (2006). "Behaviour in cultures and habitat requirements of species within the genera Loreleia and ...

https://en.wikipedia.org/wiki/Loreleia

2017] Notes: ♦ Type strain lost or not available ♪ Prokaryotes where no pure (axenic) cultures are isolated or available, i.e. ... not cultivated or can not be sustained in culture for more than a few serial passages ♠ Strains found at the National Center ...

https://en.wikipedia.org/wiki/Spirochaete

They are unusually easy to propagate in pure culture, and some 40% of the many Smittium species have been established as axenic ...

https://en.wikipedia.org/wiki/Smittium

Prokaryotes where no pure (axenic) cultures are isolated or available; i.e., they are not cultivated or cannot be sustained in ...

https://en.m.wikipedia.org/wiki/Chloroflexi_

Various fungi including Penicillium and Aspergillus species growing in axenic culture. Some penicillium mold on mandarin ...

https://en.m.wikipedia.org/wiki/Penicillium

I. Isoenzyme and soluble protein patterns of axenic cultures of S. microadriaticum. Proc R Soc Lond B 207:405-27 ... 39,0 39,1 39,2 Reimer JD, Shah MMR, Sinniger F, Yanagi K, Suda S (2010) Preliminary analyses of cultured Symbiodinium isolated ... cultured symbiotic dinoflagellates: implications for extrapolating to the intact symbiosis. J Phycol 37:900-12 ... Dinoflagellata; its culture in vitro. Palau Tropical Biological Station Studies 2:265-275 ...

https://gl.wikipedia.org/wiki/Symbiodinium

Prokaryotes where no pure (axenic) cultures are isolated or available, i.e., not cultivated or cannot be sustained in culture ... "The first 1000 cultured species of the human gastrointestinal microbiota". FEMS Microbiology Reviews. 38 (5): 996-1047. doi ...

https://en.wikipedia.org/wiki/Bacteroidetes

4-dichlorophenol both in axenic culture and in an EcM association with Pinus sylvestris. The intact rhizosphere of Pinus taeda ... There are large culture collections maintained throughout the world; however, there are only approximately 11,500 species ... Carbon uptake requires a transporter, AmMST1, that is only expressed when the fungus is cultured at low glucose levels in ... "Carbon allocation to ectomycorrhizal fungi correlates with belowground allocation in culture studies" (PDF). Ecology. 87 (3): ...

https://en.wikipedia.org/wiki/Ectomycorrhiza

It is often essential to isolate a pure culture of microorganisms. A pure (or axenic) culture is a population of cells or ... Broth culturesEdit. One method of bacterial culture is liquid culture, in which the desired bacteria are suspended in a liquid ... Major national culture collections.[4][5]. Collection Acronym Name Location ATCC American Type Culture Collection Manassas, ... Isolation of pure culturesEdit. Main article: Axenic. For single-celled eukaryotes, such as yeast, the isolation of pure ...

https://en.m.wikipedia.org/wiki/Microbiological_culture

Vacuolar form The vacuolar form is the typical cell form of Blastocystis seen in culture and is often used for the ... As yet, no publication has identified the subtypes of most of the ATCC isolates, which are mostly axenic. Researchers have ... Colony growth on solid medium colonies on solid culture medium using a synthetic medium with added supplements have both been ... A research study has reported that amoeboid forms are produced only in cultures taken from symptomatic individuals, with ...

https://en.wikipedia.org/wiki/Blastocystis

a b Prokaryotes where no pure (axenic) cultures are isolated or available, i. e. not cultivated or can not be sustained in ...

https://en.wikipedia.org/wiki/Green_sulfur_bacteria

... on the growth of anaerobic protozoa in axenic culture". The Tokai Journal of Experimental and Clinical Medicine. 15 (6): 417-23 ...

https://sq.m.wikipedia.org/wiki/Giardia_lamblia

Axenic Cell culture of the plant Physcomitrella patens on an agarplate in a Petri dish ... Virus or phage cultures require that a population of bacteria be grown in the dish first, which then becomes the culture medium ... Sonja Bäumel (2009): "Oversized petri dish". Culture of microroganisms from the artist's skin pressed onto a body-size culture ... The culture medium is often an agar plate, a layer a few mm thick of agar or agarose gel containing whatever nutrients the ...

https://en.m.wikipedia.org/wiki/Petri_dish

However, in 2009 scientists reported a technique allowing the Q-fever pathogen Coxiella burnetii to grow in an axenic culture ...

https://en.m.wikipedia.org/wiki/Intracellular_pathogen

Stool culture was considered by some researchers to be the most reliable technique, but a recent study found stool culture only ... Lanuza MD, Carbajal JA, Villar J, Mir A, Borras R (1999). "Soluble-protein and antigenic heterogeneity in axenic Blastocystis ... Protozoal culture is unavailable in most countries due to the cost and lack of trained staff able to perform protozoal culture ... Stool culture: Culturing has been shown to be a more reliable method of identifying infection. In 2006, researchers reported ...

https://en.wikipedia.org/wiki/Blastocystosis

3D cell culturing by magnetic levitation. *Cell culture. *Chemically defined medium. *Cell culture assay ...

https://en.wikipedia.org/wiki/Category:Biotechnology

D in axenic cultures under conditions of nitrogen starvation , and at least two of these ?SPM1 encoding a serine protease ... D in axenic cultures under conditions of nitrogen starvation [13,14], and at. Posted on July 24, 2017. by Proteasome inhibitor ... D in axenic cultures under conditions of nitrogen starvation [13,14], and at least two of these ?SPM1 encoding a serine ... Moreover, the up.D in axenic cultures under conditions of nitrogen starvation [13,14], and at least two of these ?SPM1 encoding ...

http://www.esiservizi.com/2017/07/24/d-in-axenic-cultures-under-conditions-of-nitrogen-starvation-1314-and-at/

Culture Strains. Trophozoites from the E. histolytica HM1: IMSS strain were cultured in TYI-S-33 medium under axenic conditions ... "CLUPS": A New Culture Medium for the Axenic Growth of Entamoeba histolytica. F. Gonzalez-Salazar,1,2 I. Meester,2 F. J. Guzmán ... We introduce a new culture medium (CLUPS) for the axenic growth of E. histolytica. CLUPS medium is a modification of the PEHPS ... Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, ...

https://www.hindawi.com/journals/jpr/2018/2796516/

Axenic Culture, Pathogenicity, and Comparison with Xylella fastidiosa of Citrus...J. E. O. de Lima , Agrocitros Citrolima Ltda ... Antisera developed against cultured bacteria from both CLS and CVC plants reacted positively against plant extracts of both ...

https://www.apsnet.org/publications/plantdisease/1998/January/Pages/82_1_94.aspx

211pgs. Fine condition softcover, as new from publisher. First Edition Third Printing. August 2014. An authoritative and comprehensive compendium of over 130 media formulations and recipes used for the propagation of orchid seeds in vitro, this treatise presents not just the hows, but the very readable wherefores and whys of the materials and techniques used. Contents: History; Chemistry: The Elements, Isotopes, Oxidation States, The Chemistry of Ions, Formula Weight; The Metric System; Stock Solutions, Overlays; Glassware: Glass, Pipettes, Volumetric Flasks, Erlenmeyer Flasks, Graduated Cylin ...

https://www.ravenroostbooks.com/products/keyword/%20Axenic%20Culture

Find out information about axenic culture. The growth of organisms of a single species in the absence of cells or living ... organisms of any other species Explanation of axenic culture ... Related to axenic culture: pure culture, monoxenic culture. ... Axenic culture , Article about axenic culture by The Free Dictionary https://encyclopedia2.thefreedictionary.com/axenic+culture ... In a re-association experiment, cells from the heterotrophic culture were added to an axenic culture of the Anabaena sp.. ...

http://encyclopedia2.thefreedictionary.com/Axenic+culture

Metabolism of the cellular slime mould Dictyostelium discoideum grown in axenic culture J. M. Ashworth; J. M. Ashworth ... J. M. Ashworth, D. J. Watts; Metabolism of the cellular slime mould Dictyostelium discoideum grown in axenic culture. Biochem J ... Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture Biochem J (September,1970) ... 5. The rate of amino acid oxidation during axenic growth of the myxamoebae is decreased when the cells are supplied with ...

https://portlandpress.com/biochemj/article-abstract/119/2/175/17595/Metabolism-of-the-cellular-slime-mould?redirectedFrom=fulltext

Of the three cultures, Ochrobactrum showed most metabolic activity on the widest range of NA surrogates. Brevundomonas and ... These pure cultures were screened for their ability to use a range of aliphatic, cyclic and aromatic NA surrogates in 96-well ... Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor ...

https://rd.springer.com/article/10.1007/s10532-015-9736-3

The Nematode C. elegans as an Animal Model to Explore Toxicology In Vivo: Solid and Axenic Growth Culture Conditions and ... Axenic culture of free‐living, parasitic and insect‐parasitic nematodes. Annu. Rev. Phytopathol. 16:131‐157. ... This unit includes protocols for culturing worms on solid and axenic media and acute and chronic exposure parameters for ... Use of CeHR Axenic Medium for Exposure and Gene Expression Studies. Reproductive Hazards Laboratory, U.S. Army Center for ...

http://www.currentprotocols.com/WileyCDA/CPUnit/refId-tx0109.html

Methods A wide range of Cd and Zn concentrations were applied to ectomycorrhizal fungi in axenic cultures (in mg L−1): 0; 1; 3 ... Cd and Zn interactions and toxicity in ectomycorrhizal basidiomycetes in axenic culture. Vinicius H. De Oliveira, Mark Tibbett ... A wide range of Cd and Zn concentrations were applied to ectomycorrhizal fungi in axenic cultures (in mg L−1): 0; 1; 3; 9; 27; ... De Oliveira VH, Tibbett M. (2018) Cd and Zn interactions and toxicity in ectomycorrhizal basidiomycetes in axenic culture. ...

https://peerj.com/articles/4478/

The work presented herein focused on determining axenic conditions for culturing and growing insect (epimastigote) forms of T. ... Axenic culture Is the Subject Area "Axenic culture" applicable to this article? Yes. No. ... Establishment of T. vivax axenic cultures and impact of serum source. We started T. vivax adaptation to axenic cultures using ... Parasite maintenance in axenic cultures and in vitro differentiation. Epimastigote cultures were initiated with the blood of ...

https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0001461

https://en.wikipedia.org/wiki/Endogone

Amoebae treated with mannitol in axenic liquid culture formed 50% TRC after 17 h but no walled cysts were found. It was ... Transfer of amoebae from liquid culture to the surface of diluted axenic agar medium resulted in the formation of walled cysts ... Previous workers reported that Physarum polycephalum amoebae cultured in liquid axenic medium were induced to form cysts by the ... Amoebae treated with mannitol in axenic liquid culture formed 50% TRC after 17 h but no walled cysts were found. It was ...

https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-4-1059

Baker, KL; Beneke, ES; Hooper, GR; Fields, WG (1977). "Host Range and Axenic Culture of the Mycoparasite Syncephalis sphaerica ...

https://en.wikipedia.org/wiki/Rhizopus_stolonifer

Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are ... Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are ... Sand Cultures Under Axenic Conditions. Rooted poplars can also be grown in sand as the supporting medium under axenic ... Cocultivation Under Axenic Conditions. Culture systems for the cultivation of poplars with L. bicolor or P. involutus under in ...

https://www.frontiersin.org/articles/10.3389/fpls.2013.00332/full

Application of genomics, transcriptomics, proteomics and metabolomics to their study in pure/mixed cultures and environmental ... with further novel taxonomic groupings known to exist based on culture-independent molecular surveys. However, the true extent ... with further novel taxonomic groupings known to exist based on culture-independent molecular surveys. However, the true extent ... Changes in fermentation end products of anaerobic fungal metabolism in axenic culture compared to co-culture with methanogens, ...

https://www.frontiersin.org/articles/10.3389/fmicb.2017.01657/full

In the current study, we describe improved ACCM-based culture methods and their use in a fully axenic system for C. burnetii ... Expansion of the Coxiella burnetii genetics tool box using axenic culture. Abstr. 24th Meet. Am. Soc. Rickettsiol., abstr. 94. ... Second, axenic culture allows recovery of some C. burnetii transformants incapable of intracellular growth. Third, ... Here, we demonstrate that mouse tissue samples containing as little as 10 NMII GE can initiate cultures in ACCM-2. Thus, axenic ...

https://aem.asm.org/content/77/11/3720?ijkey=38a2366ca7b3eb6e7ae5e8a51d1185b38b108104&keytype2=tf_ipsecsha

Axenic Isolates. Fungi and bacteria-free plates were transferred to axenic culture by placing the amoebae in PYG medium as ... DNA from amoebic cultures was extracted by placing 1-2 mL of axenic Acanthamoeba cultures directly into the Maxwell 16 Tissue ... Axenic Cultures. Monoxenic cultures were selected from areas of profuse amoebic growth. Selected pieces of agar were ... Briefly, axenic cultures of A. griffini trophozoites in the exponential phase of growth (72 h) were chilled at 4°C and ...

https://www.hindawi.com/journals/jpr/2014/256310/

... thereby obtaining an axenic culture; and inoculating the axenic culture into a culture liquid inside the PBR to culture ... inoculate the axenic culture in a culture solution inside the PBR to culture microalgae. 10. The axenic inoculation system ... installed at an upper portion of the PBR to obtain an axenic culture, and then, inoculating the axenic culture in a culture ... installed at the upper portion of the PBR to obtain an axenic culture; and inoculate the axenic culture in a culture solution ...

http://www.patentsencyclopedia.com/app/20160145556

Culture System: Axenic. Cryopreservation Harvest and Preservation *Harvest cells from a culture that is at or near peak density ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just ... Incubate the culture at 50-100 µEinsteins/m2/s irradiance at 25°C. Maintain under a 14/10h light-dark photoperiod. ...

https://atcc.org/en/Products/Cells_and_Microorganisms/Protozoa/Algae_Alphanumeric/30447.aspx

Culture System: Axenic. Cryopreservation Harvest and Preservation *To achieve the best results, set up cultures with several ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... Harvest cultures and pool when the culture that received the lowest inoculum is at or near peak density. ... To establish a culture from the frozen state, place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just ...

https://www.atcc.org/en/Products/Cells_and_Microorganisms/Protozoa/Protozoa_Alphanumeric/PRA-79.aspx

Culture System: Axenic. Subcultivation Protocol: at pH 7.0. Cryopreservation Harvest and Preservation *Harvest cells from a ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just ... Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal ...

https://www.atcc.org/en/Products/Cells_and_Microorganisms/Protozoa/Protozoa_Alphanumeric/30231.aspx

Growth and Terpenoid Production of an Axenic Culture from the Liverwort Ricciocarpos natans. Wurzel, Gerald / Becker, Hans ...

https://www.degruyter.com/view/j/znc.1990.45.issue-1-2/issue-files/znc.1990.45.issue-1-2.xml

M. tuberculosis axenic cultures. Recombinant IL-26 monomers (R&D Systems) were diluted in 10 mM sodium phosphate, pH 7.2. ... leprae in axenic culture and reduced bacteria viability. Furthermore, IL-26, when added to human monocyte-derived macrophages ... leprae (Figure 5B). Similarly, IL-26 inhibited the growth of Mycobacterium tuberculosis H37Ra in axenic culture, quantified by ... leprae was incubated with recombinant IL-26 protein in axenic culture. After 6 hours, IL-26 was detected on the surface of some ...

https://www.jci.org/articles/view/99550

Aquatic plants stimulate the growth of and biofilm formation by Mycobacterium ulcerans in axenic culture and harbor these ...

https://findanexpert.unimelb.edu.au/display/publicationS1220471

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http://www.bio.net/bionet/mm/methods/1993-October/007959.html

PCR before sample culture. Axenic culture. Cell culture detection at day 21. ... Characteristics of 27 microorganisms only isolated in the shell-vial culture by the JNSP protocol for which axenic cultures ... Presented for each microorganism are the results of 16S rRNA PCR, culture, and strain establishment in axenic medium. ... of positive shell-vial cultures. Species. No. of strains established from shell-vial culture. ...

https://jcm.asm.org/content/43/10/4993/figures-only

... the first axenic cultures of this organism to be described from Germany, were established in Bonn from faecal cysts obtained ... A total of 15 isolates of Giardia intestinalis, the first axenic cultures of this organism to be described from Germany, were ... Characterization of axenic isolates of Giardia intestinalis established from humans and animals in Germany Parasitol Res. 1998 ... intestinalis isolates can grow in axenic culture. ... cultures and both assemblage B isolates being slow growers. ...

https://pubmed.ncbi.nlm.nih.gov/9660132/?dopt=Abstract

The phytoplankton cultures examined were, Prochlorococcus (axenic and xenic), Synechococcus (axenic), Emiliania huxleyi, ... All cultures were axenic except C. leptoporous. The cultures were grown at 20-25 °C (depending on species) in an even split ... All cultures were axenic, with the exception of Porphyridium sp. and Prorocentrum sp. Cultures were bubbled with ultra-high ... It was unclear whether the cultures were axenic. Scarratt and Moore [92] examined cultures of several marine phytoplanktons: ...

http://www.mdpi.com/2218-1989/7/3/39/htm

Axenic cultures of symbiotic algae. *Modulated fluorescence of chlorophyll, kinetics (fluorometer, actinic light) ... Microbial cultures. Development of bioreactors under thermal insulation; genetic characterization and enzymatic analysis of ...

https://www.uv.es/uvweb/cavanilles-institute-biodiversity-biology/en/l-institut/equipment-1285898354573.html

Axenic Culture. Culture of an isolated organism free from any other associating or contaminating organisms. ... A shift from culture-dependent to culture-independent ide.... Metagenomic analyses of the gut microbiota associated with ... Culture. A collective expression for all behavior patterns acquired and socially transmitted through symbols. Culture includes ... Myriads of elements affect human embryo culture in vitro. These include, but not limited to, air quality, culture media, ...

https://www.bioportfolio.com/resources/pmarticle/2346725/Combined-culture-and-metagenomic-analyses-reveal-significant-shifts-in-the-composition-of.html

Antisera developed against cultured bacteria from both CLS and CVC plants reacted positively against plant extracts of both diseases in dot immunobinding assays (DIBA). (apsnet.org)
Because the particles were assumed to be bacterial pathogens, efforts were made to produce an axenic culture of the ameba isolate, and 16S rRNA PCR was performed to identify any intracellular bacteria. (thefreedictionary.com)
Aquatic plants stimulate the growth of and biofilm formation by Mycobacterium ulcerans in axenic culture and harbor these bacteria in the environment. (thefreedictionary.com)
1. The DNA, RNA, protein and carbohydrate contents of myxamoebae of Dictyostelium discoideum strain Ax-2 were measured after growth on bacteria or in various axenic media. (portlandpress.com)
When a microalgae culture contaminated with bacteria passes through the TR tube filled with the triiodide resin, a pure culture of microalgae can be prepared by using the sterilizing rate of bacteria populations, which is relatively higher than that of microalgae, due to characteristics of iodine exhibiting negative polarity. (patentsencyclopedia.com)
According to the present invention, the microalgae contaminated with bacteria are sterilized together with bacteria in external air flowing in the TR tube while passing through the TR tube, thereby causing no secondary contamination at the time of inoculation or culture. (patentsencyclopedia.com)
6. A method for culturing axenic culture of microalgae using a triiodide resin, comprising: passing a microalgae culture contaminated with a number of bacteria through a triiodide resin (TR) tube to obtain an axenic culture, wherein the TR tube includes the triiodide resin and has a resin discharge prevention part at a lower portion thereof. (patentsencyclopedia.com)
10. The axenic inoculation system according to claim 9, wherein the microalgae contaminated with bacteria is sterilized by an open triiodide resin (TR) tube. (patentsencyclopedia.com)
a Protocols (type of cell used, length of incubation, and culture revelation) were different according to the required bacteria. (asm.org)
Insight into the diversity of antibiotic resistance genes in the intestinal bacteria of shrimp Penaeus vannamei by culture-dependent and independent approaches. (bioportfolio.com)
Procedures used to prevent the introduction of fungi, bacteria, viruses, mycoplasma or other microorganisms into cell, tissue and organ cultures. (sivb.org)
Bacteria associated with cultured rotifers and artemia are detrimental to larval turbot, Scophthalmus maximus L. Aquacultural Engineering 7: 289-293. (springer.com)
One method of bacterial culture is liquid culture, in which the desired bacteria are suspended in a liquid nutrient medium, such as Luria Broth , in an upright flask. (wikipedia.org)
Liquid cultures are ideal for preparation of an antimicrobial assay in which the experimenter inoculates liquid broth with bacteria and lets it grow overnight (they may use a shaker for uniform growth). (wikipedia.org)
Once the growth medium in the petri dish is inoculated with the desired bacteria, the plates are incubated at the optimal temperature for the growing of the selected bacteria (for example, usually at 37 degrees Celsius, or the human body temperature , for cultures from humans or animals, or lower for environmental cultures). (wikipedia.org)
Our microbial cultures can confirm the absence of bacteria in samples from germ-free animals. (criver.com)
Working with an axenic culture allowed the study of the whole process of degradation of phytoplankton released DOM, simulating the release of material by these cyanobacteria in a reservoir and its utilization by the bacterial heterotrophic community as the utilization of co-cultures of phytoplankton/bacteria can make subtle changes in the released organic matter composition (Bruckner et al. (thefreedictionary.com)

Previous workers reported that Physarum polycephalum amoebae cultured in liquid axenic medium were induced to form cysts by the addition of mannitol. (microbiologyresearch.org)

Axenic cultivation of E. histolytica was first accomplished by Diamond in 1961 [ 9 ], using a diphasic medium composed of a serum-enriched agar base covered by a broth supplemented with chicken extract and vitamins. (hindawi.com)
Term used to denote the maintenance or cultivation of cells in vitro including the culture of single cells. (sivb.org)
In obtaining a culture from another laboratory, the proper designation of the culture, as originally named and described, must be maintained and any deviations in cultivation from the original must be reported in any publication. (sivb.org)
Method of cultivation: Axenic cultures maitained in liquid medium. (europa.eu)
Synxenic and attempted axenic cultivation of rotifers. (thefreedictionary.com)

donovani strain MHOM/ET/67/L82 were grown in axenic culture at 37 [degrees]C in SM medium (Cunningham 1977) at pH 5. (thefreedictionary.com)
2. Myxamoebae grown in the different axenic media have similar DNA, RNA and protein contents, but there are marked differences in the contents of glycogen and free sugars. (portlandpress.com)
Although most species will only produce spores in nature, the type species E. pisiformis can be made to sporulate in test tube culture when grown with conifer seedlings. (wikipedia.org)
The cultures are grown in a sterile synthetic liquid medium devoid of enriched supplements such as serum. (cdc.gov)
Axenic Mtb cultures were grown in Sauton's broth, a defined, minimal medium, to late-log phase. (nih.gov)
grown for two or three days in f/2 and 2f media were found to be dependent on the initial cell concentration, since the cultures started with 80 × 10 3 cells ml -1 gave better yields than those started with 40 × 10 3 cells ml -1 . (bioone.org)
There was a correlation between nutrient availability and cell yields: the two and three days-old cultures started with 40 × 10 3 cells ml -1 and grown in medium f/2 had lower concentrations than those grown in 2f medium, whereas in cultures with 80 × 10 3 cells ml -1 inoculum the yield was significantly higher only after three days. (bioone.org)
Microbiological cultures can be grown in petri dishes of differing sizes that have a thin layer of agar-based growth medium. (wikipedia.org)
To verify the response of host cells to trichomonad infection, a primary culture of bovine epithelial cells (BOECs) was grown to model host cells, following previously published procedures (Midlej et al. (scielo.br)
This study aims to compare the phytochemical composition and pharmacological properties of axenically and naturally grown liverwort L. cruciata to find out whether the axenically cultured bryophytes can be used as a substitute to naturally grown ones to meet up the demands of sample available for use in research purposes. (biomedcentral.com)
Healthy stems and leaves of ten, 30-day-old plants of S. tragus were spray-inoculated with an aqueous suspension of conidia (1.0 x 106 alpha conidia/ml plus 0.1 percent v/v polysorbate 20) harvested from 14-day-old cultures grown on 20 percent V8 juice agar. (usda.gov)
Healthy stems and leaves of ten, 30-day-old plants of S. tragus were spray-inoculated with an aqueous suspension of conidia (1.0 x 106 alpha conidia/ml) harvested from 14-day-old cultures grown on 20 percent V8 juice agar. (usda.gov)

Harvest cultures and pool when the culture that received the lowest inoculum is at or near peak density. (atcc.org)

Activities of phenol oxidizing enzymes of ectomycorrhizal fungi in axenic culture and in symbiosis with Scots pine (Pinus sylvestris L. (thefreedictionary.com)
Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are described. (frontiersin.org)
Comparison of development of axenic cultures of five species of lichen-forming fungi. (wikimedia.org)
2003 Improvement of axenic culture of arbuscular mycorrhizal fungi. (thefreedictionary.com)

Transfer of amoebae from liquid culture to the surface of diluted axenic agar medium resulted in the formation of walled cysts identical in appearance with those formed on bacterial lawns. (microbiologyresearch.org)
For the purpose of gelling the microbial culture, the medium of agarose gel ( agar ) is used. (wikipedia.org)
Stab cultures are similar to agar plates, but are formed by solid agar in a test tube. (wikipedia.org)

One set was tested by culture techniques for the presence of live organisms at the Mycoplasma Laboratory, Science Applications International Corporation, National Cancer Institute, Frederick Research and Development Center, Frederick, MD. A commercial nongovernment facility, Charles River Tektagen, tested the second set for mycoplasma DNA by polymerase chain reaction (PCR) assay. (cdc.gov)
An axenic culture may include the purposeful cocultivation of different types of cells, tissues or organisms. (sivb.org)
A microbiological culture , or microbial culture , is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. (wikipedia.org)
A pure (or axenic ) culture is a population of cells or multicellular organisms growing in the absence of other species or types. (wikipedia.org)
Traditional culture-based studies in the past have documented the trophic tendencies of various mixotrophs [ 10 - 13 ], but have not provided details on the molecular processes occurring in the organisms. (biomedcentral.com)

[0001] The present invention relates to an axenic inoculation system for microalgae using a triiodide resin (TR) tube of a triiodide resin, and a method for culturing an axenic culture. (patentsencyclopedia.com)
Intrahepatic inoculation with 1 and 2 × 10 4 axenic amebae resulted in an increase in the size of the liver abscess for a period of 2 wk and its apparent total resorption by day 17 post-inoculation, accompanied by a corresponding gain of body weight. (ajtmh.org)

Motile plasmodia are also found in some cultured species (Okamoto et al. (tolweb.org)

Better culture media could facilitate the in vitro study of microorganisms such as E. histolytica . (hindawi.com)
Here we report on the optimization of non-infective T. vivax epimastigote axenic cultures and on the process of parasite in vitro differentiation into metacyclic infective forms. (plos.org)
Myriads of elements affect human embryo culture in vitro. (bioportfolio.com)
Percent shoot regeneration from leaf explant was highest (86.66%) followed by in vitro source of explant (83.65%), B5 basal medium (83.20%) or 2.27 uM TDZ (73.70%) after 60 days of initial culture. (thefreelibrary.com)
Furthermore, we have demonstrated that curcumin inhibited the histone acetyltransferase (HAT) activity of the recombinant PfGCN5 in vitro and reduced nuclear HAT activity of the parasite in culture. (thenakedscientists.com)
Axenic culture of L. cruciata was established by propagating gemmae under in vitro condition. (biomedcentral.com)

The culture collection of algae at Indiana University. (atcc.org)
This project will focus primarily on laboratory cultures of axenic marine algae. (epa.gov)
A generalized set of conditions for culturing micro-algae (modified from Anonymous, 1991). (fao.org)
The production of high value products in micro algae for pharmaceutical use is gaining increased interest, which requires large-scale axenic culture of microalgae. (edu.au)
Algae without HKB upregulated their expression of genes coding for ammonium transporters, implying uptake of inorganic nitrogen from the culture medium when prey were unavailable. (biomedcentral.com)

and inoculating the axenic culture into a culture liquid inside the PBR to culture microalgae. (patentsencyclopedia.com)
and inoculate the axenic culture in a culture solution inside the PBR to culture microalgae. (patentsencyclopedia.com)
More particularly, a TR tube is fabricated using a triiodide resin and attached on an upper portion of a photobioreactor (PBR), and used for passing a culture solution containing microalgae, pure liquid and air through the TR tube. (patentsencyclopedia.com)
This project aims to bridge this gap, creating a scalable single-use photobioreactor for the production of axenic microalgae capable of producing high value products. (edu.au)

The vaccine, which is administered subcutaneously over an 18-month schedule, is derived from sterile, acellular filtrates of microaerophilic cultures of the avirulent, nonencapsulated V770-NP1-R strain of Bacillus anthracis . (cdc.gov)
Strain IP-106 of Entamoeba histolytica was isolated 12 yr ago from a case of amebic dysentery and has been maintained in axenic culture for the past 11 yr. (ajtmh.org)
strain PWH3a was infected with a lytic virus (PWH3a-P1) and the resulting 36.0 µ mol L −1 of dissolved organic N (DON) in the lysate was added to cultures containing cyanobacteria ( Synechococcus sp. (biogeosciences.net)

Among the topics are a novel protocol for model calibration in biological wastewater treatment, the characterization of pure cultures isolated from sulfamethoxazole-acclimated activated sludge with respect to taxonomic identification and sulfamethoxazole biodegradation potential, the bacterial consortium and axenic cultures isolated from activated sewage sludge for biodegradation and imidazolium-based ionic liquid, and the scenario analysis of nutrient removal from municipal wastewater by microbial biofilms. (thefreedictionary.com)

In any published description of a culture, one must make every attempt to publish the characterization or history of the culture. (sivb.org)

The work presented herein focused on determining axenic conditions for culturing and growing insect (epimastigote) forms of T. vivax and prompting their differentiation into metacyclic forms that are infectious for the mammalian host. (plos.org)
However, a major impediment to validating this model is a poor understanding of the actual nutrient conditions encountered by M. oryzae during infection, what nutrients can be acquired from the host, and how closely axenic growth in synthetic minimal media mimics the nutrient conditions of the plant. (esiservizi.com)
Here, we describe modified ACCM and culture conditions that support improved growth of C. burnetii and their use in genetic transformation and pathogen isolation from tissue samples. (asm.org)
Bacterial additives that consistently enhance rotifer growth under synxenic culture conditions 2. (springer.com)
A total of 21 out of 79 amoeba peptidase genes analysed were found to be significantly expressed under standard axenic culture conditions whereas the remaining are not expressed or at very low levels only. (biomedcentral.com)
Under standard culture conditions or upon heat-stress only a relatively small number of these genes is significantly expressed and only very few variations become apparent between various clinical E. histolytica isolates, calling into question the importance of these enzymes in E. histolytica pathogenicity. (biomedcentral.com)
Appropriate culture conditions, media, and the effect of hormones on growth and development were studied. (biomedcentral.com)
Although culturing plant tissues and organs under axenic conditions was first established and profitably employed in bryophytes, especially mosses [ 13 ], bryophytes did not retain for long their rightful place as a highly favored research object. (biomedcentral.com)
For assays, the parasites were maintained in axenic conditions at 36.5[degrees]C for 30 days by serial subcultivation in TYI-S-33, PEHPS, or CLUPS medium. (thefreedictionary.com)
Production of algal biomass, chlorophyll, starch and lipids using aquaculture wastewater under axenic and non-axenic conditions. (thefreedictionary.com)

A completely axenic C. burnetii genetic transformation system was developed using ACCM-2 that allowed isolation of transformants in about 2 1/2 weeks. (asm.org)

Lichen growth forms cannot be recapitulated in the laboratory by culturing the plant and fungal partners together. (sciencemag.org)

To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters. (hindawi.com)
Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. (hindawi.com)

[4] [5] Culture collection are also repositories of type strains . (wikipedia.org)

Continuous growth of bloodstream forms of Trypanosoma brucei brucei in an axenic culture system containing a low concentration of serum. (thefreedictionary.com)

We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. (hindawi.com)
In 1988, our group reported an in-house developed axenic culture medium, PEHPS [ 14 ]. (hindawi.com)
The axenic culture medium 199 supplemented by 5% of calf newborn serum was used. (thefreedictionary.com)
About 100 [micro]l of the culture medium with 105 amastigotes from axenic culture with or without a serial drug dilution was seeded in 96-well microtiter plates. (thefreedictionary.com)
NMII was successfully cultured in ACCM-2 when medium was inoculated with as little as 10 genome equivalents contained in tissue homogenates from infected SCID mice. (asm.org)
Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 mL of fresh ATCC medium 712 in a T-25 tissue culture flask or plastic 16 x 125 mm screw-capped test tube. (atcc.org)
Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal slant for medium 2154). (atcc.org)
KEYWORDS: biomass production, Culture medium, Dunaliella sp. (bioone.org)
There was, however, a significant difference in the average cell size, with diameters ranging from 9.30 +/- 0.184 to 11.11 +/- 0.22 microns and from 9.17 +/- 0.05 to 11.25 +/- 0.24 microns for cells growing in Excell 401 serum-free medium in spinner flask cultures and in TNM-FH medium supplemented with 10% FBS in tissue flask cultures, respectively. (biomedsearch.com)
For example, a throat culture is taken by scraping the lining of tissue in the back of the throat and blotting the sample into a medium to be able to screen for harmful microorganisms, such as Streptococcus pyogenes , the causative agent of strep throat. (wikipedia.org)
Host cell cultures were exposed to a parasite:BOECS cell ratio of 5:1 for 12 h in 37ºC ( Fig. 1C, D ). Cells were equilibrated in incubation medium for 5 min at 37ºC (5% CO 2 ) prior to parasite addition. (scielo.br)

Ideally, cultured parasites should present the same growth and virulence characteristics as their counterparts in their natural habitat [ 8 ]. (hindawi.com)
Development of media formulations permitting axenic culture of marine pathogens has enabled scientists to study the growth of parasites, their virulence factors and host-parasite interactions. (thefreedictionary.com)
To characterise the inflammatory response induced by these parasites, primary cultures of bovine oviduct epithelial cells were exposed to either T. vaginalis or T. foetus. (scielo.br)
The supernatants of cultures with parasites or without (controls) were collected and cytokine levels were quantified with specific kits for measuring IL-10 (Becton Dickinson, Franklin Lakes, NJ, USA), which were used according to the manufacturers' instructions. (scielo.br)
They are digenetic parasites: the flagellated promastigote forms, which can be derived in axenic culture, differentiate within the alimentary tracts of sandfly vectors from a replicating procyclic to a nonreplicating, infectious metacyclic stage, whereas obligately intracellular amastigotes live and replicate in mammalian mononuclear phagocytes, such as macrophages ( 1 ). (asm.org)

Microbial cultures. (uv.es)
Although these procedures are used to prevent microbial contamination of cultures, they also prevent cross contamination of cell cultures as well. (sivb.org)
Microbial cultures are foundational and basic diagnostic methods used extensively as a research tool in molecular biology . (wikipedia.org)
Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both. (wikipedia.org)
Microbial culture collections focus on the acquisition, authentication, production, preservation, catalogueing and distribution of viable cultures of standard reference microorganisms , cell lines and other materials for research in microbial systematics . (wikipedia.org)
Anaerobic agents are the most difficult to detect from oxygen-containing environments and, therefore, it is critical that anaerobic collection culture media, anaerobic chambers, and powerful microbial identification technologies be utilized to ensure accurate results. (criver.com)

It is often essential to isolate a pure culture of microorganisms. (wikipedia.org)

The aim of this study was to determine whether T. foetus and T. vaginalis stimulated the release of the cytokine interleukin (IL)-10 from cultured bovine epithelial cells. (scielo.br)

A morphological description must await achieving axenic culture , but the partial sequence of the SSUrDNA has been given the GenBank accession numbers AF228362 and AF228363 for the V1, and V2 and V3 regions of the SSUrDNA, respectively. (thefreedictionary.com)

The recent description of a method for axenic (host cell-free) propagation of C. burnetii is a significant technological advance that will facilitate biochemical and genetic studies of the pathogen ( 22 ). (asm.org)
Clonal propagation through tissue culture offers an alternative approach to vegetative propagation. (thefreelibrary.com)

Molecular detection of Coxiella burnetii in heart valve tissue from patients with culture-negative infective endocarditis. (pubfacts.com)
Coxiella burnetii is a common cause of blood culture-negative infective endocarditis (IE). (pubfacts.com)

Polymorphic behavior of Ulva lactuca (Chlorophyta) in axenic culture. (algaebase.org)

Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. (hindawi.com)
This unit includes protocols for culturing worms on solid and axenic media and acute and chronic exposure parameters for Parkinson's disease-associated toxins and hemin chloride. (currentprotocols.com)
Various specific recipes for algal culture media are described by Vonshak (1986). (fao.org)
The complexity and cost of the above culture media often excludes their use for large-scale culture operations. (fao.org)

The availability of the complete genome sequence of the nematode C. elegans , coupled with the worm's size, growth rate, ease of culturing, and the realization that basic biological mechanisms and disease processes between worms and humans are highly conserved, makes this genetically tractable model a remarkable opportunity to dissect and identify in vivo the cellular processes involved in toxin‐induced cell dysregulation and death. (currentprotocols.com)

Here, we examined the diagnostic utility of Q fever polymerase chain reaction (PCR) of formalin-fixed heart valve tissue from patients with blood culture-negative IE who underwent heart valve surgery. (pubfacts.com)
In tissue culture, cytokinins play an important role as they promote cell division and develop meristematic centers leading to the formation of organs, mainly shoots (Peeters et al. (thefreelibrary.com)

A pure culture may originate from a single cell or single organism, in which case the cells are genetic clones of one another. (wikipedia.org)

2006). Even HeLa cells cultured with this parasite have been reported to secrete some cytokines (Chang et al. (scielo.br)

A total of 15 isolates of Giardia intestinalis, the first axenic cultures of this organism to be described from Germany, were established in Bonn from faecal cysts obtained from human and animal stool specimens. (nih.gov)
The data indicate that genetically based metabolic differences may determine how rapidly G. intestinalis isolates can grow in axenic culture. (nih.gov)

Nitrogen flow in a chemostat culture of the rotifer Brachionus plicatilis . (springer.com)

The role played by specific probiotic candidates is still indistinct and further research is required to ascertain their significance in rotifer culture. (springer.com)
In this study, euryhaline rotifer Brachionus rotundiformis (SS-type) was used to evaluate the effect of two commercial probiotic products on rotifer density, reproductive parameters and unionized ammonia concentration for a twelve-day culture period. (springer.com)

Integrate a source of irradiance, optimised gas (CO2/O2) exchange and temperature control into GE Healthcare's Xcellerex bioreactor platform, set up a flexible sensor array for monitoring of the culture. (edu.au)

These pure cultures were screened for their ability to use a range of aliphatic, cyclic and aromatic NA surrogates in 96-well microtiter plates using water-soluble tetrazolium redox dyes (Biolog Redox Dye H) as the indicator of metabolic activity. (springer.com)
Pure cultures from these isolations were sent to the quarantine facility of the Foreign Disease-Weed Science Research Unit, USDA, ARS, Fort Detrick, MD for testing. (usda.gov)

Cattle infectious subspecies in axenic asynchronous culture. (lshtm.ac.uk)
Charles River offers comprehensive, accurate, and sensitive detection of infectious agents using both aerobic and anaerobic cultures of samples obtained from the isolator environment, individual cages, and directly from colony animals. (criver.com)

1978. Method for the simultaneous establishment of many axenic cultures of Paramecium . (springer.com)

In cell cultures, the cells are no longer organized into tissues. (sivb.org)

Here we show that curcumin , a polyphenolic organic molecule derived from turmeric, inhibits chloroquine-resistant Plasmodium falciparum growth in culture in a dose dependent manner with an IC(50) of approximately 5 microM. (thenakedscientists.com)

More particularly, a TR tube is fabricated using a triiodide resin and a method for culturing an axenic culture. (patentsencyclopedia.com)

The Effect of Initial Cell and Nutrient Concentrations on the Growth and Biomass Production of Outdoor Cultures of Dunaliella sp. (bioone.org)
The final concentrations of non-axenic outdoor mass cultures of Dunaliella sp. (bioone.org)
Concentrations of cells in phytoplankton cultures are generally higher than those found in nature. (fao.org)

Shell-vial culture using the JNSP protocol from January 1996 to September 2003. (asm.org)

In a re-association experiment, cells from the heterotrophic culture were added to an axenic culture of the Anabaena sp. (thefreedictionary.com)
5. The rate of amino acid oxidation during axenic growth of the myxamoebae is decreased when the cells are supplied with glucose. (portlandpress.com)
Harvest cells from a culture that is at or near peak density by centrifugation at 700-800 x g for 5 min. (atcc.org)
There is hardly a field of biological investigation in which culturing of such cells is not employed. (sivb.org)
Cells, or cultures derived from them, which will grow, survive, or maintain function only when attached to a surface such as glass or plastic. (sivb.org)
The percentage of cells plated (seeded, inoculated) which attach to the surface of the culture vessel within a specified period of time. (sivb.org)
The term cell line implies that cultures from it consist of lineages of cells originally present in the primary culture. (sivb.org)
Batch culture experiments without the flagellate demonstrated that the elongation of C. acidovorans PX54 cells was dependent on their growth rate. (asm.org)

There are several types of bacterial culture methods that are selected based on the agent being cultured and the downstream use. (wikipedia.org)
Improved Methods for the Axenic Culture of Puccinia recondita f. sp. (apsnet.org)

This confusion is especially acute in the field of vertebrate, invertebrate and plant cell culture. (sivb.org)
maritima, any treatment effects must have originated in the plant rather than an epiphyte, as only axenic plants were used. (thefreedictionary.com)

To date, nine anaerobic fungal genera have been described, with further novel taxonomic groupings known to exist based on culture-independent molecular surveys. (frontiersin.org)

Moreover, skin microbiome studies have heavily relied on 16S rRNA sequencing data in the absence of bacterial culture. (bioportfolio.com)

An E. festucae Δ bemA mutant was defective in hyphal morphogenesis and growth in culture and in planta . (pnas.org)

The live food(s) would be cultured in a dedicated vessel. (wetwebmedia.com)

Diagnosis is based on serologic analysis, culture, and molecular biology. (cdc.gov)

Organisms16

Diseases4

Chemicals and Drugs10

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Psychiatry and Psychology1

Phenomena and Processes2

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture3

Humanities2

Information Science3

Health Care5

Geographicals3

Leishmania major parasite stage-dependent host cell invasion and immune evasion. (1/14)

Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota. (2/14)

The genetic toolbox for Leishmania parasites. (3/14)

Epidemiologic consequences of microvariation in Mycobacterium tuberculosis. (4/14)

Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi. (5/14)

Targeting host syntaxin-5 preferentially blocks Leishmania parasitophorous vacuole development in infected cells and limits experimental Leishmania infections. (6/14)

Antileishmanial activity of warifteine: a bisbenzylisoquinoline alkaloid isolated from Cissampelos sympodialis Eichl. (Menispermaceae). (7/14)

The response of dark septate endophytes (DSE) to heavy metals in pure culture. (8/14)

Axenic Culture

Claviceps

Peptones

Culture Media

Myxomycetes

Entamoeba histolytica

Eukaryota

Biodegradation, Environmental

Phylogeny

DNA, Ribosomal

Molecular Sequence Data

RNA, Ribosomal, 16S

Nitrogen

Starvation

Microalgae

Iodides

Photobioreactors

Patents as Topic

Biofuels

Chlorophyta

Nitrate Reductase (NAD(P)H)

Cladocera

Lysobacter

Hydrocarbons

Chara

Tritrichomonas foetus

Tritrichomonas

Protozoan Infections, Animal

Trichomonas

Protozoan Infections

Trichomonadida

Trichomonas vaginalis

Commerce

Taxes

Prescription Fees

Philately

Great Britain

Germany

Medicine, Arabic

Western Australia

Telefacsimile

Genome, Mitochondrial

Sheep

Agricultural Irrigation

DNA, Mitochondrial

Problem Solving

Skin Manifestations

Bacteria

Polymerase Chain Reaction

Leishmania major parasite stage-dependent host cell invasion and immune evasion. (1/14)

Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota. (2/14)

The genetic toolbox for Leishmania parasites. (3/14)

Epidemiologic consequences of microvariation in Mycobacterium tuberculosis. (4/14)

Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi. (5/14)

Targeting host syntaxin-5 preferentially blocks Leishmania parasitophorous vacuole development in infected cells and limits experimental Leishmania infections. (6/14)

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Menispermaceae

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