Axenic Culture

Claviceps

Peptones

Culture Media

Myxomycetes

Entamoeba histolytica

Eukaryota

Biodegradation, Environmental

Phylogeny

DNA, Ribosomal

Molecular Sequence Data

RNA, Ribosomal, 16S

Students

Faculty, Medical

Faculty

Students, Medical

Research

Mechlorethamine

Publications

Axenic

Germ-free animal

Phytoplasma

Cypripedium reginae

Vernalization

Sceptridium multifidum

Dendrolycopodium obscurum

Warwick L. Nicholas

Endogone

Tylopilus felleus

Caenorhabditis briggsae

Micromonas

Limnoperdon

Rhizopus stolonifer

Symbiodinium

Gnotobiosis

Amoebidium

Balamuthia mandrillaris

Sceptridium dissectum

Physarum polycephalum

Spanish Bank of Algae

Thraustochytrids

Aspergillus austroafricanus

Phaeocystis

Trebouxia

Phycotoxin

Chorioactis

Penicillium expansum

Root-knot nematode

Foreign grain beetle

Microbiological culture

Moss bioreactor

Opalinidae

Symbiotaphrina

Intracellular parasite

Mycobacterium leprae

Koch's postulates

Robert W. Lichtwardt

Blastocystosis

Mycena galopus

Cyanobacteria

Loreleia

Ectocarpus

Amoebidiidae

Campylobacterota

Paramoebidium

Pleomorphism (microbiology)

George Poinar Jr.

Cultivation3

• To complete TYI-S-33 medium for use in axenic cultivation add 2.0 ml of vitamin mix #18 and 10-15 ml of heat inactivated adult bovine serum to each 88 ml of TYI broth. (lshtm.ac.uk)
• Current axenic algae cultivation systems lack robustness, are expensive and generally have marginal energy returns. (biomedcentral.com)
• Present approaches typically focus on the axenic cultivation of a single strain, such as Spirulina , Nannochloropsis , Chlorella or Dunaliella . (biomedcentral.com)

Histolytica3

• E histolytica can be cultured for research and susceptibility purposes (especially for emetine susceptibility) but does not contribute to diagnostic purposes. (medscape.com)
• In most cases 13 ml of TYI-S-33 per tube is the correct amount as inocula for axenic E. histolytica are generally small in established cultures. (lshtm.ac.uk)
• One characteristic of what were once known as 'non-pathogenic E. histolytica ' isolates and are now known as E. dispar is that they are refractory to axenization by the classic technique for obtaining axenic E. histolytica cultures. (lshtm.ac.uk)

Grown5

• The cultures are grown in a sterile synthetic liquid medium devoid of enriched supplements such as serum. (cdc.gov)
• The culture was grown in the medium, and under the environmental conditions, described for the test. (europa.eu)
• In contrast, cell-dense cultures grown under optimal conditions appear more sensitive to exposure during extended incubation, likely as a result of increased rate of encounter between cyanobacteria and nanoparticles at high cell densities. (frontiersin.org)
• 2. Evaluation of antioxidant and anticancer activity of crude extract and different fractions of Chlorella vulgaris axenic culture grown under various concentrations of copper ions. (nih.gov)
• In order to extract the algicidal compounds, BS02 was prepared in distilled water from cultures grown on AC1 solid medium, was used to inoculate 1000 mL flasks, containing 500 mL AC1 liquid medium. (woofahs.com)

Organisms3

• One set was tested by culture techniques for the presence of live organisms at the Mycoplasma Laboratory, Science Applications International Corporation, National Cancer Institute, Frederick Research and Development Center, Frederick, MD. A commercial nongovernment facility, Charles River Tektagen, tested the second set for mycoplasma DNA by polymerase chain reaction (PCR) assay. (cdc.gov)
• Culture of an isolated organism free from any other associating or contaminating organisms. (nih.gov)
• UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. (utex.org)

Algae4

• One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. (utex.org)
• 2. Isolation and culture methods of algae. (nig.ac.jp)
• The biphasic or soil-water culture method for growing algae and flagellata. (nig.ac.jp)
• In Cultures and Collections of Algae, Eds. (nig.ac.jp)

Platensis2

• Fresh- and salt-water forms of Spirulina platensis in axenic cultures. (limnology-journal.org)
• I) On the pure culture of Spirulina platensis. (nig.ac.jp)

Alga3

• The test species was the unicellular green alga Selenastrum capricornutum ATCC 22662 from laboratory cultures maintained under axenic conditions. (europa.eu)
• A 3 day old culture of the alga in the exponential growth phase was used as inoculum for the test. (europa.eu)
• Growth characteristics of freshwater red tide alga, Peridinium based on axenic culture. (nig.ac.jp)

Agar3

• For agar cultures , strains are transferred to the surface of fresh agar slants in 20 mm diamter glass screw-cap tubes in preparation for shipment. (utex.org)
• Agar and liquid cultures are packaged within a Styrofoam box and shipped the same day they are packaged. (utex.org)
• Components and Strategies Bacterial Ethnicities BS02 was cultured in sea agar 2216E (pH 7.47.8) in 25C for 2448 h, and was preserved in ?80C in marine broth with 20% (v/v) glycerol. (woofahs.com)

Exponential growth phase1

• Assays for Algicidal Activity The algicidal activity was carried out in 24-well plates (2 mL of cultures in the exponential growth phase were assigned to each well). (woofahs.com)

ATCC1

• Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen. (atcc.org)

Concentrations1

• The extracted fractions or purified components dissolved in Dimethyl sulfoxide (DMSO) were added into cultures at different final concentrations in triplicate. (woofahs.com)

Strains1

• The utility of Wolbachia in control applications would be enhanced if the microbe could be experimentally manipulated by genetic engineering to express selectable markers, which in turn will be advanced by improving manipulation of Wolbachia in cell lines and expanding the diversity of Wolbachia strains that can be investigated in culture. (encyclopedia.pub)

Flocculating bacteria1

• 11. The effect of recycling culture medium after harvesting of Chlorella vulgaris biomass by flocculating bacteria on microalgal growth and the functionary mechanism. (nih.gov)

Species1

• On the same time, we are building a Cyanobacteria bank, where a large variety of marine species are going to be identified, isolated and cultured in axenic condition for research purposes. (szn.it)

Sterile2

• The vaccine, which is administered subcutaneously over an 18-month schedule, is derived from sterile, acellular filtrates of microaerophilic cultures of the avirulent, nonencapsulated V770-NP1-R strain of Bacillus anthracis . (cdc.gov)
• It turns out that "axenic" flies-those raised in a sterile environment-leap, at the age of about 10 days, to a kind of metabolic middle age (30 days or so) and remain there until the end, which is itself delayed by about 20 percent compared to siblings raised under normal, non-sterile conditions. (nih.gov)

Medium8

• On each day of analysis, the sample preparation procedure was validated by fortifying triplicate 20 ml aliquots of culture medium with 50 ul of a 400 mg L-1 stock of Aquapel 364, prepared in acetone. (europa.eu)
• This study was run with a culture medium and a solvent control together with a nominal Aquapel 364 concentration of 1.0 mg I-1. (europa.eu)
• The test solution was prepared from this stock solution by addition of appropriate volumes to 1 L of culture medium and stirring thoroughly. (europa.eu)
• A solvent control was similarly prepared by the addition of 0.1 ml of acetone to 1 litre of culture medium, to give a solvent concentration of 100 ul/L. The control consisted of culture medium only. (europa.eu)
• All Cultures were maintained in f/2 medium (without silicate) prepared with natural seawater [34] at 201C under a 1212 h lightCdark cycle with a light intensity of 50 mmol photons m?2s?1. (woofahs.com)
• The pre-culture (incubated at 28C for 1 day in an orbital incubator set to 150 rpm) was used to inoculate (5% v/v) a total volume of 25 L culture medium having the same composition as the pre-culture. (woofahs.com)
• In contrast, E. coli and many familiar Gram-negative pathogens of humans classified as gamma-proteobacteria can be cultured in liquid medium and plated on solid media as free-living microbes. (encyclopedia.pub)
• Establishment of synthetic culture medium [Junsuibaiyoho niyoru tansuiakashio Peridinium no zosyokutokusei no kaiseki-goseibaichi no kakuritsu]. (nig.ac.jp)

Laboratory1

• Laboratory culture and morphology of Colacium vesiculosum Ehrb. (nig.ac.jp)

Conditions3

• Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. (utex.org)
• After a stock culture is established, subcultures may be used for testing other conditions. (utex.org)
• Competitive dominance of the cyanobacterium Microcystis aeruginosa in nutrient-rich culture conditions with special reference to dissolved inorganic carbon uptake. (limnology-journal.org)

Genetic1

• Bartonella melophagi in blood of domestic sheep (Ovis aries) and sheep keds (Melophagus ovinus) from the southwestern US: Cultures, genetic characterization, and ecological connections. (cdc.gov)

Biomass1

• To harvest algal biomass from a suspended culture, the culture is commonly concentrated via sedimentation or flocculation to around 1% (dry basis) then further concentration using centrifugation to 20% (dry basis) [ 16 , 17 ]. (biomedcentral.com)

Media2

• Part I. Methods and culture media. (nig.ac.jp)
• Culture media for Euglena gracilis. (nig.ac.jp)

Liquid1

• For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. (atcc.org)

Animals1

• and (3) metabolites not produced in axenic animals. (who.int)

Data2

• Coupling feeding activity, growth rates and molecular data shows dietetic needs of Ciona robusta (Ascidiacea, Phlebobranchia) in automatic culture plants. (szn.it)
• Our data underline the high prevalence of infective endocarditis caused by Bartonella quintana in northern Africa and the role of serologic and molecular tools for the diagnosis of blood culture-negative endocarditis. (cdc.gov)

Cell1

• Mycoplasma contamination is considered to be a potential problem for vaccines that are produced in cell cultures. (cdc.gov)

Pure culture1

• In this study, apple juice was fermented using Hanseniaspora osmophila X25-5 in pure culture as well as mixed culture with Torulaspora quercuum X24-4, which was inoculated simultaneously or sequentially. (bvsalud.org)

Treatment1

• The rate of blood culture-negative endocarditis in Algeria is as high as 76% ( 2 ), which leads to difficulty in antimicrobial treatment. (cdc.gov)

Collection1

• Collection and mass culture [Shiryo no saisyu to baiyo]. (nig.ac.jp)

Cases4

• Causative agents were detected by blood cultures in 48 cases. (cdc.gov)
• All 62 blood culture-negative endocarditis cases were tested by serologic or molecular methods or both. (cdc.gov)
• Most cases of blood culture-negative endocarditis have been thought to be caused by previous antimicrobial therapy. (cdc.gov)
• But within few years of successive culture, farmers faced a bitter experience, most of the culture ponds became hypernutrified which in extreme cases caused eutrophication by algal population due to continuous deposition of nutrients from unutilized natural food, excessive supplementary feed, uneaten portion of feed and excretory products. (medcraveonline.com)

Results1

• 1:800) and 7 minor criteria (positive blood cultures, fever, previous heart disease, arterial embolism, positive results on serologic examination for endocarditis bacterial pathogens, immunologic disorders, and atypical but compatible findings on echocardiography) ( 12 ). (cdc.gov)