Axenic culture. Medical search

Culture of an isolated organism free from any other associating or contaminating organisms.

A genus of ascomycetous fungi, family Clavicipitaceae, order Hypocreales, parasitic on various grasses (POACEAE). The sclerotia contain several toxic alkaloids. Claviceps purpurea on rye causes ergotism.

Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)

Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.

A division of organisms that exist vegetatively as complex mobile plasmodia, reproduce by means of spores, and have complex life cycles. They are now classed as protozoa but formerly were considered fungi.

A species of parasitic protozoa causing ENTAMOEBIASIS and amebic dysentery (DYSENTERY, AMEBIC). Characteristics include a single nucleus containing a small central karyosome and peripheral chromatin that is finely and regularly beaded.

One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.

Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.

The relationships of groups of organisms as reflected by their genetic makeup.

DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.

An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.

Lengthy and continuous deprivation of food. (Stedman, 25th ed)

A non-taxonomic term for unicellular microscopic algae which are found in both freshwater and marine environments. Some authors consider DIATOMS; CYANOBACTERIA; HAPTOPHYTA; and DINOFLAGELLATES as part of microalgae, even though they are not algae.

Inorganic binary compounds of iodine or the I- ion.

Devices for generating biological products that use light as the energy source. They are used for controlled BIOMASS production such as growing cyanobacteria, mosses, or algae.

Exclusive legal rights or privileges applied to inventions, plants, etc.

Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).

A phylum of photosynthetic EUKARYOTA bearing double membrane-bound plastids containing chlorophyll a and b. They comprise the classical green algae, and represent over 7000 species that live in a variety of primarily aquatic habitats. Only about ten percent are marine species, most live in freshwater.

An iron-sulfur and MOLYBDENUM containing FLAVOPROTEIN that catalyzes the oxidation of nitrite to nitrate. This enzyme can use either NAD or NADP as cofactors. It is a key enzyme that is involved in the first step of nitrate assimilation in PLANTS; FUNGI; and BACTERIA. This enzyme was formerly classified as EC 1.6.6.2.

A suborder of CRUSTACEA, order Diplostraca, comprising the water fleas. They are benthic filter feeders that consume PHYTOPLANKTON. The body is laterally compressed and enclosed in a bivalved carapace, from which the head extends.

A genus of gram-negative, rod-shaped, gliding bacteria in the family XANTHOMONADACEAE. Strongly proteolytic, it is involved in lysing a variety of microorganisms.

A genus of green plants in the family CHARACEAE, phylum STREPTOPHYTA. They have a strong garlic-like odor and are an important food source for waterfowl.

A species of flagellate parasitic EUKARYOTE. It possesses a long undulating membrane that is bordered on its outer margin by a flagellum that becomes free posteriorly. This organism causes infections in cows that could lead to temporary infertility or abortion.

A genus of flagellate EUKARYOTES possessing three long anterior flagella.

Infections with unicellular organisms formerly members of the subkingdom Protozoa. The infections may be experimental or veterinary.

A genus of parasitic flagellate EUKARYOTES distinguished by the presence of four anterior flagella, an undulating membrane, and a trailing flagellum.

Infections with unicellular organisms formerly members of the subkingdom Protozoa.

An order of parasitic EUKARYOTES typically having four to six flagella. True cysts are known in very few species. Trichomonadida were formerly members of the class Zoomastigophora in the old five kingdom paradigm.

A species of TRICHOMONAS that produces a refractory vaginal discharge in females, as well as bladder and urethral infections in males.

The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)

Governmental levies on property, inheritance, gifts, etc.

The charge levied on the consumer for drugs or therapy prescribed under written order of a physician or other health professional.

Study of stamps or postal markings. It usually refers to the design and commemorative aspects of the stamp.

Traditional Arabic methods used in medicine in the ARAB WORLD.

A state in western Australia. Its capital is Perth. It was first visited by the Dutch in 1616 but the English took possession in 1791 and permanent colonization began in 1829. It was a penal settlement 1850-1888, became part of the colonial government in 1886, and was granted self government in 1890. (From Webster's New Geographical Dictionary, 1988, p1329)

A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.

The genetic complement of MITOCHONDRIA as represented in their DNA.

Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.

The routing of water to open or closed areas where it is used for agricultural purposes.

Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.

A learning situation involving more than one alternative from which a selection is made in order to attain a specific goal.

Dermatologic disorders attendant upon non-dermatologic disease or injury.

One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

Leishmania major parasite stage-dependent host cell invasion and immune evasion. (1/14)

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Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota. (2/14)

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The genetic toolbox for Leishmania parasites. (3/14)

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Epidemiologic consequences of microvariation in Mycobacterium tuberculosis. (4/14)

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Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi. (5/14)

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Targeting host syntaxin-5 preferentially blocks Leishmania parasitophorous vacuole development in infected cells and limits experimental Leishmania infections. (6/14)

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Antileishmanial activity of warifteine: a bisbenzylisoquinoline alkaloid isolated from Cissampelos sympodialis Eichl. (Menispermaceae). (7/14)

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The response of dark septate endophytes (DSE) to heavy metals in pure culture. (8/14)

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Axenic culture "Germ Free Mouse Facility". University of Michigan. Archived from the original on 30 October 2015. Reyniers JA ( ...

https://en.wikipedia.org/wiki/Germ-free_animal

Contaldo, N.; Bertaccini, A.; Paltrinieri, S.; Windsor, H.M.; Windsor, G.D. (2012). "Axenic culture of plant pathogenic ... used for bacteria that cannot be cultured). Phytoplasma taxonomy is complicated because the organisms cannot be cultured; ... Tissue culture can be used to produce healthy clones of phytoplasma-infected plants. Cryotherapy (i.e., the freezing of plant ... Since their discovery, phytoplasmas have resisted all attempts at in vitro culture in any cell-free medium; routine cultivation ...

https://en.wikipedia.org/wiki/Phytoplasma

Specific-pathogen-free (SPF) Axenic culture Gnotobiotics. Schoeb, Trenton R.,, Eaton, Kathryn A. London. 11 August 2017. ISBN ...

https://en.wikipedia.org/wiki/Gnotobiosis

Sokolski, K.; Dovholuk, A.; Dovholuk, L.; Faletra, P. (1997). "Axenic seed culture and micropropagation of Cypripedium reginae ...

https://en.wikipedia.org/wiki/Vernalization

"Gametophytes of Botrychium multifidum as Grown in Axenic Culture". American Fern Journal. 68 (3): 71-75. doi:10.2307/1546452. ...

https://en.wikipedia.org/wiki/Sceptridium_multifidum

Gametophytes of Lycopodium obscurum as grown in axenic culture. Canadian Journal of Botany. 55. pp. 563-567. Hickey, R. James. ...

https://en.wikipedia.org/wiki/Dendrolycopodium_obscurum

Berch SM, Castellano MA (1986). "Sporulation of Endogone pisiformis in axenic and monaxenic culture". Mycologia. 78 (2): 292- ... 1990). "Taxonomy, culture, and mycorrhizal associations of some zygosporic Endogonaceae". Mycological Research. 94 (2): 173-178 ... pisiformis can be made to sporulate in test tube culture when grown with conifer seedlings. Endogone was first circumscribed by ...

https://en.wikipedia.org/wiki/Endogone

The mycelium of Tylopilus felleus can be grown in axenic culture, on agar containing growth medium. The fungus can form fruit ... 1974). "Fruitbody formation by Tylopilus fellus in axenic culture". Mycologia. 66 (1): 197-202. doi:10.2307/3758474. JSTOR ... There are few Boletaceae species known to fruit in culture, as ectomycorrhizal fungi tend to not fruit when separated from ...

https://en.wikipedia.org/wiki/Tylopilus_felleus

... axenic) cultures are isolated or available; i.e., they are not cultivated or cannot be sustained in culture for more than a few ...

https://en.wikipedia.org/wiki/Chloroflexi_(phylum)

This work was important in the development of axenic culture methods. C. briggsae can often be found in compost, garden beds, ...

https://en.wikipedia.org/wiki/Caenorhabditis_briggsae

In 2005, a monoclonal culture of the strain was isolated. The axenic strain is available from the Center for Culture of Marine ... The antibiotic susceptibility was determined using a single strain of M. pusilla with the purpose to produce axenic cultures to ... Cottrell, Matthew T.; Suttle, Curtis A. (1 June 1993). "Production of Axenic Cultures of Micromonas Pusilla (Prasinophyceae) ... This strain has been continuously cultured for two decades and is available from the Roscoff Culture Collection. ...

https://en.wikipedia.org/wiki/Micromonas

The tips of the hyphae were used to obtain axenic cultures; the fungus can grow on a variety of media commonly used to grow ... Escobar grew cultures of the fungus by placing fresh fruit bodies on agar containing growth medium with an extract of horse ... Experimental observations on the development of the fruit body, based on the growth on the fungus in pure culture, suggest that ... Mycologist Dennis McCabe studied the development of the fruit body using specimens grown in pure culture. Starting out as a ...

https://en.wikipedia.org/wiki/Limnoperdon

Baker, KL; Beneke, ES; Hooper, GR; Fields, WG (1977). "Host Range and Axenic Culture of the Mycoparasite Syncephalis sphaerica ...

https://en.wikipedia.org/wiki/Rhizopus_stolonifer

I. Isoenzyme and Soluble Protein Patterns of Axenic Cultures of Symbiodinium microadriaticum". Proceedings of the Royal Society ... In culture, the discarded walls accumulate at the bottom of the culture vessel. It is not known what becomes of the walls from ... Culturing is a selective process, and many Symbiodinium isolates growing on artificial media are not typical of the species ... All cultured isolates (i.e. strains) are capable of phenotypic adjustment in their capacity for light harvesting (i.e. ...

https://en.wikipedia.org/wiki/Symbiodinium

Therefore, axenic cultures of trichomycetes are highly valuable for obtaining pure DNA samples. As a result, the phylogenetic ... to be obtained in axenic culture, allowing for detailed studies of its nutritional requirements, cell wall composition, and ... Amoebidium appalachense was also obtained in axenic culture, and subsequent molecular analyses supported its relationship with ... Culture and nutrition of Amoebidium parasiticum. American Journal of Botany 49: 193-199. Trotter, M. J., and Whisler, H. C. ...

https://en.wikipedia.org/wiki/Amoebidium

Whittier, P. (1981). "Spore Germination and Young Gametophyte Development of Botrychium and Ophioglossum in Axenic Culture". ...

https://en.wikipedia.org/wiki/Sceptridium_dissectum

In the 1990s a group of high school students in New Hampshire made progress on axenic culture from seed and were able to obtain ... 1997). "Axenic Seed Culture and Micropropagation of Cypripedium reginae" Selbyana 18(2): 172-182. "Minnesota State Symbols". ... in Axenic Seed Culture, Bulletin of America Association for the Advancement of Sciences, Annual meeting, pp. A-112 AAAS Annual ... It is difficult to raise from seed, taking many months to germinate in sterile culture. ...

https://en.wikipedia.org/wiki/Cypripedium_reginae

These cultures have strain numbers preceded by BEA. Non-axenic cultures are marked with "B" at the end of the strain number. ... BEA has been member of the European Culture Collection Organization (ECCO) since 2001 and of the World Federation for Culture ... "Spanish Type Culture Collection (University of Valencia)" (in Spanish). Retrieved 26 December 2011. "European Culture ... gDNA "à la carte": BEA is able to extract genomic DNA from microalgae cultures from a wide range of volumes. Normally kits (i.e ...

https://en.wikipedia.org/wiki/Spanish_Bank_of_Algae

"Metabolites from the Fungal Endophyte Aspergillus austroafricanus in Axenic Culture and in Fungal-Bacterial Mixed Cultures". ...

https://en.wikipedia.org/wiki/Aspergillus_austroafricanus

Production of DMS from dissolved DMSP in axenic cultures of the marine phytoplankton species Phaeocystis sp., Mar. Ecol. Prog. ... not in culture), P. cordata, and P. rex. Three species (P. globosa, P. pouchetii, and P. antarctica) are associated with bloom ...

https://en.wikipedia.org/wiki/Phaeocystis

... in axenic culture on defined media. Retrieved from https://ir.library.oregonstate.edu/xmlui/handle/1957/44218 Archibald, P. A ... doi:10.1007/bf00940436 Ahmadjian, V. (1967). A Guide to the Algae Occurring as Lichen Symbionts: Isolation, Culture, Cultural ...

https://en.wikipedia.org/wiki/Trebouxia

... multiseries cultures which were completely axenic (bacteria-free), produce less domoic acid than P. multiseries cultures which ... "Enhancement of domoic acid production by reintroducing bacteria to axenic cultures of the diatom Pseudo-nitzschia multiseries ... For example, in a mixed batch culture the target species may die or have reduced growth rates due to competition for nutrients ... All methods of detecting phycotoxins involve extraction of the candidate toxin from a phytoplankton culture; therefore, it's ...

https://en.wikipedia.org/wiki/Phycotoxin

... they were able to grow the organism in axenic cultures from single-spore isolates of C. geaster. Until the one fungus, one name ... Finally, only Japanese specimens can be grown in culture-the spores of Texan material have not been successfully germinated on ...

https://en.wikipedia.org/wiki/Chorioactis

"Effect of ammonium ions on egg hatching and second-stage juveniles of Meloidogyne incognita in axenic tomato root culture". ...

https://en.wikipedia.org/wiki/Root-knot_nematode

An axenic culture of TM7 from the oral cavity was reported in 2014 but no sequence or culture was made available. Along with ... "Candidatus Saccharibacteria oral taxon TM7x (ID 241438) - BioProject - NCBI". Soro, V. (2014). "Axenic Culture of a Candidate ... a Recently Recognized Major Lineage of the Domain Bacteria with No Known Pure-Culture Representatives". Applied and ...

https://en.wikipedia.org/wiki/Saccharibacteria

Prokaryotes where no pure (axenic) cultures are isolated or available; i.e., they are not cultivated or cannot be sustained in ...

https://en.m.wikipedia.org/wiki/Chloroflexi_

The culture is kept under lighting with temperature and pH value held constant. The culture medium-often a minimal medium- ... Algae bioreactor Bioreactor Molecular farming Photobioreactor Physcomitrella patens Hohe, A.; Reski, R. (2005). "From axenic ... At this point the culture has to be diluted with fresh medium if it is intended for further use. According to the intended ... Once the density of the culture has reached a certain threshold, the lack of nutrients and the increasing concentration of ...

https://en.wikipedia.org/wiki/Moss_bioreactor

... of the early laboratory work on Caenorhabditis nematodes was directed towards the establishment of a defined axenic culture ... Ellsworth C. Dougherty; Eder Lindsay Hansen; Warwick L. Nicholas; J. Anthony Mollett; Evangeline A. Yarwood (1959). "Axenic ... from a laboratory culture of the closely related nematode Caenorhabditis briggsae. However much ...

https://en.wikipedia.org/wiki/History_of_research_on_Caenorhabditis_elegans

Amino acid uptake and metabolism by larvae of the marine worm Urechis caupo (Echiura), a new species in axenic culture. ...

https://en.wikipedia.org/wiki/Donal_T._Manahan

Various fungi including Penicillium and Aspergillus species growing in axenic culture. Some penicillium mold on mandarin ...

https://en.m.wikipedia.org/wiki/Penicillium

I. Isoenzyme and soluble protein patterns of axenic cultures of S. microadriaticum. Proc R Soc Lond B 207:405-27 ... 39,0 39,1 39,2 Reimer JD, Shah MMR, Sinniger F, Yanagi K, Suda S (2010) Preliminary analyses of cultured Symbiodinium isolated ... cultured symbiotic dinoflagellates: implications for extrapolating to the intact symbiosis. J Phycol 37:900-12 ... Dinoflagellata; its culture in vitro. Palau Tropical Biological Station Studies 2:265-275 ...

https://gl.wikipedia.org/wiki/Symbiodinium

However, in 2009 scientists reported a technique allowing the Q-fever pathogen Coxiella burnetii to grow in an axenic culture ...

https://en.m.wikipedia.org/wiki/Intracellular_pathogen

... on the growth of anaerobic protozoa in axenic culture". The Tokai Journal of Experimental and Clinical Medicine. 15 (6): 417-23 ...

https://sq.m.wikipedia.org/wiki/Giardia_lamblia

Prokaryotes where no pure (axenic) cultures are isolated or available, i.e., not cultivated or cannot be sustained in culture ... "The first 1000 cultured species of the human gastrointestinal microbiota". FEMS Microbiology Reviews. 38 (5): 996-1047. doi ...

https://en.wikipedia.org/wiki/Bacteroidetes

a b Prokaryotes where no pure (axenic) cultures are isolated or available, i. e. not cultivated or can not be sustained in ...

https://en.wikipedia.org/wiki/Green_sulfur_bacteria

Axenic Cell culture of the plant Physcomitrella patens on an agarplate in a Petri dish ... Virus or phage cultures require that a population of bacteria be grown in the dish first, which then becomes the culture medium ... Sonja Bäumel (2009): "Oversized petri dish". Culture of microroganisms from the artist's skin pressed onto a body-size culture ... The culture medium is often an agar plate, a layer a few mm thick of agar or agarose gel containing whatever nutrients the ...

https://en.m.wikipedia.org/wiki/Petri_dish

3D cell culturing by magnetic levitation. *Cell culture. *Chemically defined medium. *Cell culture assay ...

https://en.wikipedia.org/wiki/Category:Biotechnology

It is often essential to isolate a pure culture of microorganisms. A pure (or axenic) culture is a population of cells or ... Broth culturesEdit. One method of bacterial culture is liquid culture, in which the desired bacteria are suspended in a liquid ... Major national culture collections.[4][5]. Collection Acronym Name Location ATCC American Type Culture Collection Manassas, ... Isolation of pure culturesEdit. Main article: Axenic. For single-celled eukaryotes, such as yeast, the isolation of pure ...

https://en.m.wikipedia.org/wiki/Microbiological_culture

4-dichlorophenol both in axenic culture and in an EcM association with Pinus sylvestris. The intact rhizosphere of Pinus taeda ... There are large culture collections maintained throughout the world; however, there are only approximately 11,500 species ... Carbon uptake requires a transporter, AmMST1, that is only expressed when the fungus is cultured at low glucose levels in ... "Carbon allocation to ectomycorrhizal fungi correlates with belowground allocation in culture studies" (PDF). Ecology. 87 (3): ...

https://en.wikipedia.org/wiki/Ectomycorrhiza

It is often essential to isolate a pure culture of microorganisms. A pure (or axenic) culture is a population of cells or ... Colony-forming unit Blood culture Microbial dark matter Microbial Food Cultures Screening cultures Sputum culture Synchronous ... Stab cultures are most commonly used for short-term storage or shipment of cultures. Microbial culture collections focus on the ... the isolation of pure cultures uses the same techniques as for bacterial cultures. Pure cultures of multicellular organisms are ...

https://en.wikipedia.org/wiki/Microbiological_culture

in axenic medium" (PDF). Zoological Science. 18 (3): 381-7. doi:10.2108/zsj.18.381. Archived from the original (PDF) on 2017-10 ... Successful culturing of opalines in artificial media for periods of 1 month or more has been reported. This technique will aid ...

https://en.wikipedia.org/wiki/Opalinidae

This is similar to how viruses cannot grow in axenic culture: viruses must be living to replicate, so the culture is not a ... The microorganism must be isolated from a diseased organism and grown in pure culture. The cultured microorganism should cause ... be grown in pure culture. Viruses also require host cells to grow and reproduce and therefore cannot be grown in pure cultures ... Some pathogens cannot be cultured in the lab, and some pathogens only cause disease in humans. In summary, an infectious agent ...

https://en.wikipedia.org/wiki/Koch's_postulates

He grew over 150 trichomycete isolates in axenic culture, a critical step for experimental studies on nutritional modes and ...

https://en.wikipedia.org/wiki/Robert_W._Lichtwardt

Stool culture was considered by some researchers to be the most reliable technique, but a recent study found stool culture only ... Lanuza MD, Carbajal JA, Villar J, Mir A, Borras R (1999). "Soluble-protein and antigenic heterogeneity in axenic Blastocystis ... Protozoal culture is unavailable in most countries due to the cost and lack of trained staff able to perform protozoal culture ... Stool culture: Culturing has been shown to be a more reliable method of identifying infection. In 2006, researchers reported ...

https://en.wikipedia.org/wiki/Blastocystosis

Grown in axenic culture in the laboratory, the fungus mycelium has been shown to degrade (in addition to lignin and cellulose) ... a potent antifungal metabolite first isolated from submerged cultures of Pterula species in 1997. The antifungal activity of ...

https://en.wikipedia.org/wiki/Mycena_galopus

... axenic) cultures are isolated or available, i.e. not cultivated or can not be sustained in culture for more than a few serial ...

https://en.wikipedia.org/wiki/Epsilonproteobacteria

... penetrates the rhizoids of liverworts and may form a type of symbiosis with them, but in axenic culture tests, L. ... Bresinsky, A. & Schötz, A. (2006). "Behaviour in cultures and habitat requirements of species within the genera Loreleia and ...

https://en.wikipedia.org/wiki/Loreleia

... of the host and grow in axenic culture, at least some species may be facultative symbionts. The "amoeb-" prefix refers to the ...

https://en.wikipedia.org/wiki/Amoebidiidae

Culture Strains. Trophozoites from the E. histolytica HM1: IMSS strain were cultured in TYI-S-33 medium under axenic conditions ... "CLUPS": A New Culture Medium for the Axenic Growth of Entamoeba histolytica. F. Gonzalez-Salazar,1,2 I. Meester,2 F. J. Guzmán ... We introduce a new culture medium (CLUPS) for the axenic growth of E. histolytica. CLUPS medium is a modification of the PEHPS ... Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, ...

https://www.hindawi.com/journals/jpr/2018/2796516/

Axenic Culture, Pathogenicity, and Comparison with Xylella fastidiosa of Citrus...J. E. O. de Lima , Agrocitros Citrolima Ltda ... Antisera developed against cultured bacteria from both CLS and CVC plants reacted positively against plant extracts of both ...

https://www.apsnet.org/publications/plantdisease/1998/January/Pages/82_1_94.aspx

211pgs. Fine condition softcover, as new from publisher. First Edition Third Printing. August 2014. An authoritative and comprehensive compendium of over 130 media formulations and recipes used for the propagation of orchid seeds in vitro, this treatise presents not just the hows, but the very readable wherefores and whys of the materials and techniques used. Contents: History; Chemistry: The Elements, Isotopes, Oxidation States, The Chemistry of Ions, Formula Weight; The Metric System; Stock Solutions, Overlays; Glassware: Glass, Pipettes, Volumetric Flasks, Erlenmeyer Flasks, Graduated Cylin ...

https://www.ravenroostbooks.com/products/keyword/%20Axenic%20Culture

Find out information about axenic culture. The growth of organisms of a single species in the absence of cells or living ... organisms of any other species Explanation of axenic culture ... Related to axenic culture: pure culture, monoxenic culture. ... Axenic culture , Article about axenic culture by The Free Dictionary https://encyclopedia2.thefreedictionary.com/axenic+culture ... In a re-association experiment, cells from the heterotrophic culture were added to an axenic culture of the Anabaena sp.. ...

http://encyclopedia2.thefreedictionary.com/Axenic+culture

Metabolism of the cellular slime mould Dictyostelium discoideum grown in axenic culture J. M. Ashworth; J. M. Ashworth ... J. M. Ashworth, D. J. Watts; Metabolism of the cellular slime mould Dictyostelium discoideum grown in axenic culture. Biochem J ... Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture Biochem J (September,1970) ... 5. The rate of amino acid oxidation during axenic growth of the myxamoebae is decreased when the cells are supplied with ...

https://portlandpress.com/biochemj/article-abstract/119/2/175/17595/Metabolism-of-the-cellular-slime-mould?redirectedFrom=fulltext

Of the three cultures, Ochrobactrum showed most metabolic activity on the widest range of NA surrogates. Brevundomonas and ... These pure cultures were screened for their ability to use a range of aliphatic, cyclic and aromatic NA surrogates in 96-well ... Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor ...

https://rd.springer.com/article/10.1007/s10532-015-9736-3

The Nematode C. elegans as an Animal Model to Explore Toxicology In Vivo: Solid and Axenic Growth Culture Conditions and ... Axenic culture of free‐living, parasitic and insect‐parasitic nematodes. Annu. Rev. Phytopathol. 16:131‐157. ... This unit includes protocols for culturing worms on solid and axenic media and acute and chronic exposure parameters for ... Use of CeHR Axenic Medium for Exposure and Gene Expression Studies. Reproductive Hazards Laboratory, U.S. Army Center for ...

http://www.currentprotocols.com/WileyCDA/CPUnit/refId-tx0109.html

Methods A wide range of Cd and Zn concentrations were applied to ectomycorrhizal fungi in axenic cultures (in mg L−1): 0; 1; 3 ... Cd and Zn interactions and toxicity in ectomycorrhizal basidiomycetes in axenic culture. Vinicius H. De Oliveira, Mark Tibbett ... A wide range of Cd and Zn concentrations were applied to ectomycorrhizal fungi in axenic cultures (in mg L−1): 0; 1; 3; 9; 27; ... De Oliveira VH, Tibbett M. (2018) Cd and Zn interactions and toxicity in ectomycorrhizal basidiomycetes in axenic culture. ...

https://peerj.com/articles/4478/

Amoebae treated with mannitol in axenic liquid culture formed 50% TRC after 17 h but no walled cysts were found. It was ... Transfer of amoebae from liquid culture to the surface of diluted axenic agar medium resulted in the formation of walled cysts ... Previous workers reported that Physarum polycephalum amoebae cultured in liquid axenic medium were induced to form cysts by the ... Amoebae treated with mannitol in axenic liquid culture formed 50% TRC after 17 h but no walled cysts were found. It was ...

https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-4-1059

The work presented herein focused on determining axenic conditions for culturing and growing insect (epimastigote) forms of T. ... Axenic culture Is the Subject Area "Axenic culture" applicable to this article? Yes. No. ... Establishment of T. vivax axenic cultures and impact of serum source. We started T. vivax adaptation to axenic cultures using ... Parasite maintenance in axenic cultures and in vitro differentiation. Epimastigote cultures were initiated with the blood of ...

https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0001461

Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are ... Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are ... Sand Cultures Under Axenic Conditions. Rooted poplars can also be grown in sand as the supporting medium under axenic ... Cocultivation Under Axenic Conditions. Culture systems for the cultivation of poplars with L. bicolor or P. involutus under in ...

https://www.frontiersin.org/articles/10.3389/fpls.2013.00332/full

The axenic cultures were successfully subcultured to fresh medium. Some produced spores in culture. The colonies were capable ... The axenic cultures were successfully subcultured to fresh medium. Some produced spores in culture. The colonies were capable ... Host : parasite interactions of rusts and axenic culture of Melampsora species Lane, William D. Abstract. The thesis is ... Axenic cultures were established using colonies isolated in this way as inoculum. The results in Chapter IV are an extension ...

https://open.library.ubc.ca/soa/cIRcle/collections/ubctheses/831/items/1.0100056

D in axenic cultures under conditions of nitrogen starvation , and at least two of these ?SPM1 encoding a serine protease ... D in axenic cultures under conditions of nitrogen starvation [13,14], and at. Posted on July 24, 2017. by Proteasome inhibitor ... D in axenic cultures under conditions of nitrogen starvation [13,14], and at least two of these ?SPM1 encoding a serine ... Moreover, the up.D in axenic cultures under conditions of nitrogen starvation [13,14], and at least two of these ?SPM1 encoding ...

http://www.esiservizi.com/2017/07/24/d-in-axenic-cultures-under-conditions-of-nitrogen-starvation-1314-and-at/

Application of genomics, transcriptomics, proteomics and metabolomics to their study in pure/mixed cultures and environmental ... with further novel taxonomic groupings known to exist based on culture-independent molecular surveys. However, the true extent ... with further novel taxonomic groupings known to exist based on culture-independent molecular surveys. However, the true extent ... Changes in fermentation end products of anaerobic fungal metabolism in axenic culture compared to co-culture with methanogens, ...

https://www.frontiersin.org/articles/10.3389/fmicb.2017.01657/full

The earliest axenic cultures were of bacteria or unicellular eukaryotes, but axenic cultures of many multicellular organisms ... identifying cultures that are no longer axenic.[citation needed] As axenic cultures are derived from very few organisms, or ... Axenic cultures of microorganisms are typically prepared by subculture of an existing mixed culture. This may involve use of a ... Axenic cultures are usually checked routinely to ensure that they remain axenic. One standard approach with microorganisms is ...

https://en.wikipedia.org/wiki/Axenic

Axenic Isolates. Fungi and bacteria-free plates were transferred to axenic culture by placing the amoebae in PYG medium as ... DNA from amoebic cultures was extracted by placing 1-2 mL of axenic Acanthamoeba cultures directly into the Maxwell 16 Tissue ... Axenic Cultures. Monoxenic cultures were selected from areas of profuse amoebic growth. Selected pieces of agar were ... Briefly, axenic cultures of A. griffini trophozoites in the exponential phase of growth (72 h) were chilled at 4°C and ...

https://www.hindawi.com/journals/jpr/2014/256310/

Axenic culture of cardiac valves was positive for 9 samples. The growth of 2 coagulase-negative Staphylococcus, 2 Streptococcus ... Blood Cultures. Blood cultures identified 48 microorganisms (Table 2). Of the 22 Streptococcus spp. cultures, 5 Streptococcus ... Axenic Culture. Thirty-eight excised cardiac valves were examined. If macroscopic lesions of infective endocarditis were ... Blood Cultures. Either Castaneda Aer/Anaer (Bio-Rad, Marnes-La-Coquette, France) or broth for blood culture (Institut Pasteur ...

https://wwwnc.cdc.gov/eid/article/11/2/04-0668_article

... thereby obtaining an axenic culture; and inoculating the axenic culture into a culture liquid inside the PBR to culture ... inoculate the axenic culture in a culture solution inside the PBR to culture microalgae. 10. The axenic inoculation system ... installed at an upper portion of the PBR to obtain an axenic culture, and then, inoculating the axenic culture in a culture ... installed at the upper portion of the PBR to obtain an axenic culture; and inoculate the axenic culture in a culture solution ...

http://www.patentsencyclopedia.com/app/20160145556

Culture System: Axenic. Cryopreservation Harvest and Preservation *Harvest cells from a culture that is at or near peak density ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just ... Incubate the culture at 50-100 µEinsteins/m2/s irradiance at 25°C. Maintain under a 14/10h light-dark photoperiod. ...

https://atcc.org/en/Products/Cells_and_Microorganisms/Protozoa/Algae_Alphanumeric/30447.aspx

Culture System: Axenic. Cryopreservation Harvest and Preservation *To achieve the best results, set up cultures with several ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... Harvest cultures and pool when the culture that received the lowest inoculum is at or near peak density. ... To establish a culture from the frozen state, place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just ...

https://www.atcc.org/en/Products/Cells_and_Microorganisms/Protozoa/Protozoa_Alphanumeric/PRA-79.aspx

Culture System: Axenic. Subcultivation Protocol: at pH 7.0. Cryopreservation Harvest and Preservation *Harvest cells from a ... Frozen Cultures:. -70°C for 1 week; liquid N2 vapor for long term storage. Freeze-dried Cultures:. 2-8°C. Live Cultures:. See ... To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just ... Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal ...

https://www.atcc.org/en/Products/Cells_and_Microorganisms/Protozoa/Protozoa_Alphanumeric/30231.aspx

Growth and Terpenoid Production of an Axenic Culture from the Liverwort Ricciocarpos natans. Wurzel, Gerald / Becker, Hans ...

https://www.degruyter.com/view/j/znc.1990.45.issue-1-2/issue-files/znc.1990.45.issue-1-2.xml

M. tuberculosis axenic cultures. Recombinant IL-26 monomers (R&D Systems) were diluted in 10 mM sodium phosphate, pH 7.2. ... leprae in axenic culture and reduced bacteria viability. Furthermore, IL-26, when added to human monocyte-derived macrophages ... leprae (Figure 5B). Similarly, IL-26 inhibited the growth of Mycobacterium tuberculosis H37Ra in axenic culture, quantified by ... leprae was incubated with recombinant IL-26 protein in axenic culture. After 6 hours, IL-26 was detected on the surface of some ...

https://www.jci.org/articles/view/99550

Aquatic plants stimulate the growth of and biofilm formation by Mycobacterium ulcerans in axenic culture and harbor these ...

https://findanexpert.unimelb.edu.au/display/publicationS1220471

Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi Apoptotic-like activity of staurosporine in ... Apoptosis , Enzyme Inhibitors/pharmacology , Staurosporine/pharmacology , Trypanosoma/drug effects , Axenic Culture , Flow ... For access to these features we have used the flow cytometry and fluorescence microscopy with cultures in the stationary phase ... axenic cultures of Trypanosoma evansi / Actividad proapoptótica de la estaurosporina en cultivos axénicos de Trypanosoma evansi ...

https://search.bvsalud.org/gim/resource/en/lil-625263

ESTABLISHMENT OF AXENIC CULTURE ft.pdf Restricted to Registered users only Download (14MB) ... Establishment of Axenic Culture and Induction Of Embryogenic Callus from Floral Explants of Local Cocoa (Theobroma (J Cacao L ... Nuramalia, Binti Kamal Abidin Shah (2013) Establishment of Axenic Culture and Induction Of Embryogenic Callus from Floral ... S Agriculture , SB Plant culture. Divisions:. Academic Faculties, Institutes and Centres , Faculty of Resource Science and ...

https://ir.unimas.my/id/eprint/27406/

Previous message: axenic cultures *Next message: Searching.. * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] ...

http://www.bio.net/bionet/mm/methods/1993-October/007959.html

PCR before sample culture. Axenic culture. Cell culture detection at day 21. ... Characteristics of 27 microorganisms only isolated in the shell-vial culture by the JNSP protocol for which axenic cultures ... Presented for each microorganism are the results of 16S rRNA PCR, culture, and strain establishment in axenic medium. ... of positive shell-vial cultures. Species. No. of strains established from shell-vial culture. ...

https://jcm.asm.org/content/43/10/4993/figures-only

The phytoplankton cultures examined were, Prochlorococcus (axenic and xenic), Synechococcus (axenic), Emiliania huxleyi, ... All cultures were axenic except C. leptoporous. The cultures were grown at 20-25 °C (depending on species) in an even split ... All cultures were axenic, with the exception of Porphyridium sp. and Prorocentrum sp. Cultures were bubbled with ultra-high ... It was unclear whether the cultures were axenic. Scarratt and Moore [92] examined cultures of several marine phytoplanktons: ...

http://www.mdpi.com/2218-1989/7/3/39/htm

Antisera developed against cultured bacteria from both CLS and CVC plants reacted positively against plant extracts of both diseases in dot immunobinding assays (DIBA). (apsnet.org)
Because the particles were assumed to be bacterial pathogens, efforts were made to produce an axenic culture of the ameba isolate, and 16S rRNA PCR was performed to identify any intracellular bacteria. (thefreedictionary.com)
Aquatic plants stimulate the growth of and biofilm formation by Mycobacterium ulcerans in axenic culture and harbor these bacteria in the environment. (thefreedictionary.com)
1. The DNA, RNA, protein and carbohydrate contents of myxamoebae of Dictyostelium discoideum strain Ax-2 were measured after growth on bacteria or in various axenic media. (portlandpress.com)
The earliest axenic cultures were of bacteria or unicellular eukaryotes, but axenic cultures of many multicellular organisms are also possible. (wikipedia.org)
When a microalgae culture contaminated with bacteria passes through the TR tube filled with the triiodide resin, a pure culture of microalgae can be prepared by using the sterilizing rate of bacteria populations, which is relatively higher than that of microalgae, due to characteristics of iodine exhibiting negative polarity. (patentsencyclopedia.com)
According to the present invention, the microalgae contaminated with bacteria are sterilized together with bacteria in external air flowing in the TR tube while passing through the TR tube, thereby causing no secondary contamination at the time of inoculation or culture. (patentsencyclopedia.com)
6. A method for culturing axenic culture of microalgae using a triiodide resin, comprising: passing a microalgae culture contaminated with a number of bacteria through a triiodide resin (TR) tube to obtain an axenic culture, wherein the TR tube includes the triiodide resin and has a resin discharge prevention part at a lower portion thereof. (patentsencyclopedia.com)
10. The axenic inoculation system according to claim 9, wherein the microalgae contaminated with bacteria is sterilized by an open triiodide resin (TR) tube. (patentsencyclopedia.com)
a Protocols (type of cell used, length of incubation, and culture revelation) were different according to the required bacteria. (asm.org)
Insight into the diversity of antibiotic resistance genes in the intestinal bacteria of shrimp Penaeus vannamei by culture-dependent and independent approaches. (bioportfolio.com)
How the host cell compensates for these losses remains unknown, because the presence of bacteria in all available P. chromatophora cultures excluded elucidation of the full metabolic capacity and occurrence of HGT in this species. (pnas.org)
Here we generated a high-quality transcriptome and draft genome assembly from the first bacteria-free P. chromatophora culture to deduce rules that govern organelle integration into cellular metabolism. (pnas.org)
Procedures used to prevent the introduction of fungi, bacteria, viruses, mycoplasma or other microorganisms into cell, tissue and organ cultures. (sivb.org)
Bacteria associated with cultured rotifers and artemia are detrimental to larval turbot, Scophthalmus maximus L. Aquacultural Engineering 7: 289-293. (springer.com)
One method of bacterial culture is liquid culture, in which the desired bacteria are suspended in a liquid nutrient medium, such as Luria Broth , in an upright flask. (wikipedia.org)
Liquid cultures are ideal for preparation of an antimicrobial assay in which the experimenter inoculates liquid broth with bacteria and lets it grow overnight (they may use a shaker for uniform growth). (wikipedia.org)
Once the growth medium in the petri dish is inoculated with the desired bacteria, the plates are incubated at the optimal temperature for the growing of the selected bacteria (for example, usually at 37 degrees Celsius, or the human body temperature , for cultures from humans or animals, or lower for environmental cultures). (wikipedia.org)
Our microbial cultures can confirm the absence of bacteria in samples from germ-free animals. (criver.com)

Previous workers reported that Physarum polycephalum amoebae cultured in liquid axenic medium were induced to form cysts by the addition of mannitol. (microbiologyresearch.org)

Axenic cultures were established using colonies isolated in this way as inoculum. (ubc.ca)
Subculture selection may also involve manually sampling the target organism from an uncontaminated growth front in an otherwise mixed culture, and using this as an inoculum source for the subculture. (wikipedia.org)
Harvest cultures and pool when the culture that received the lowest inoculum is at or near peak density. (atcc.org)
There was a correlation between nutrient availability and cell yields: the two and three days-old cultures started with 40 × 10 3 cells ml -1 and grown in medium f/2 had lower concentrations than those grown in 2f medium, whereas in cultures with 80 × 10 3 cells ml -1 inoculum the yield was significantly higher only after three days. (bioone.org)

The culture collection of algae at Indiana University. (atcc.org)
This project will focus primarily on laboratory cultures of axenic marine algae. (epa.gov)
A generalized set of conditions for culturing micro-algae (modified from Anonymous, 1991). (fao.org)
The production of high value products in micro algae for pharmaceutical use is gaining increased interest, which requires large-scale axenic culture of microalgae. (edu.au)
Algae without HKB upregulated their expression of genes coding for ammonium transporters, implying uptake of inorganic nitrogen from the culture medium when prey were unavailable. (biomedcentral.com)

Axenic cultivation of E. histolytica was first accomplished by Diamond in 1961 [ 9 ], using a diphasic medium composed of a serum-enriched agar base covered by a broth supplemented with chicken extract and vitamins. (hindawi.com)
Term used to denote the maintenance or cultivation of cells in vitro including the culture of single cells. (sivb.org)
In obtaining a culture from another laboratory, the proper designation of the culture, as originally named and described, must be maintained and any deviations in cultivation from the original must be reported in any publication. (sivb.org)
Method of cultivation: Axenic cultures maitained in liquid medium. (europa.eu)

In biology, axenic describes the state of a culture in which only a single species, variety, or strain of organism is present and entirely free of all other contaminating organisms. (wikipedia.org)
This may involve use of a dilution series, in which a culture is successively diluted to the point where subsamples of it contain only a few individual organisms, ideally only a single individual (in the case of an asexual species). (wikipedia.org)
In the case of an asexual species derived from a single individual, the resulting culture should consist of identical organisms (though processes such as mutation and horizontal gene transfer may introduce a degree of variability). (wikipedia.org)
A pure (or axenic ) culture is a population of cells or multicellular organisms growing in the absence of other species or types. (wikipedia.org)
Comparison of development of axenic cultures of five species of lichen-forming fungi. (wikimedia.org)
Motile plasmodia are also found in some cultured species (Okamoto et al. (tolweb.org)

Continuous growth of bloodstream forms of Trypanosoma brucei brucei in an axenic culture system containing a low concentration of serum. (thefreedictionary.com)
Trypanosoma evansi es un hemoparásito, el cual es el agente causal de la surra ( tripanosomiasis ) en mamíferos , perteneciente al orden Kinetoplastidae. (bvsalud.org)
Este estudio se oriento a caracterizar la muerte celular similar a apoptosis en cultivos in vitro de Trypanosoma evansi a través del uso del inductor esturosporina. (bvsalud.org)
El efecto apoptótico de la estaurosporina en Trypanosoma evansi fue evaluado a una concentración de 20 nM. (bvsalud.org)

Activities of phenol oxidizing enzymes of ectomycorrhizal fungi in axenic culture and in symbiosis with Scots pine (Pinus sylvestris L. (thefreedictionary.com)

donovani strain MHOM/ET/67/L82 were grown in axenic culture at 37 [degrees]C in SM medium (Cunningham 1977) at pH 5. (thefreedictionary.com)
2. Myxamoebae grown in the different axenic media have similar DNA, RNA and protein contents, but there are marked differences in the contents of glycogen and free sugars. (portlandpress.com)
Axenic Mtb cultures were grown in Sauton's broth, a defined, minimal medium, to late-log phase. (nih.gov)
grown for two or three days in f/2 and 2f media were found to be dependent on the initial cell concentration, since the cultures started with 80 × 10 3 cells ml -1 gave better yields than those started with 40 × 10 3 cells ml -1 . (bioone.org)
Microbiological cultures can be grown in petri dishes of differing sizes that have a thin layer of agar-based growth medium. (wikipedia.org)
To verify the response of host cells to trichomonad infection, a primary culture of bovine epithelial cells (BOECs) was grown to model host cells, following previously published procedures (Midlej et al. (scielo.br)
This study aims to compare the phytochemical composition and pharmacological properties of axenically and naturally grown liverwort L. cruciata to find out whether the axenically cultured bryophytes can be used as a substitute to naturally grown ones to meet up the demands of sample available for use in research purposes. (biomedcentral.com)

Better culture media could facilitate the in vitro study of microorganisms such as E. histolytica . (hindawi.com)
Here we report on the optimization of non-infective T. vivax epimastigote axenic cultures and on the process of parasite in vitro differentiation into metacyclic infective forms. (plos.org)
The axenic culture of some pathogens is complicated because they normally thrive within host tissues which exhibit properties that are difficult to replicate in vitro. (wikipedia.org)
Myriads of elements affect human embryo culture in vitro. (bioportfolio.com)
Percent shoot regeneration from leaf explant was highest (86.66%) followed by in vitro source of explant (83.65%), B5 basal medium (83.20%) or 2.27 uM TDZ (73.70%) after 60 days of initial culture. (thefreelibrary.com)
Furthermore, we have demonstrated that curcumin inhibited the histone acetyltransferase (HAT) activity of the recombinant PfGCN5 in vitro and reduced nuclear HAT activity of the parasite in culture. (thenakedscientists.com)
Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (scielo.br)
Fresh spores were inoculated in vitro in different culture strengths of Knop's nutrient solution (one-fourth strength, half strength, full strength, one and a half strength and double strength), in order to evaluate the effects of this solution on spore germination and on the development of external protonema. (scielo.br)
The results of this study demonstrate the potential of in vitro culture techniques for bryophyte spore studies and germplasm preservation. (scielo.br)
Axenic culture of L. cruciata was established by propagating gemmae under in vitro condition. (biomedcentral.com)

Transfer of amoebae from liquid culture to the surface of diluted axenic agar medium resulted in the formation of walled cysts identical in appearance with those formed on bacterial lawns. (microbiologyresearch.org)
All the pustules grew for the first two weeks and after 4 months about 30% of them became established as vegetative axenic culture colonies on the agar medium. (ubc.ca)

Growth systems to cultivate poplars under axenic conditions in agar and sand cultures with and without mycorrhizal fungi are described. (frontiersin.org)

and inoculating the axenic culture into a culture liquid inside the PBR to culture microalgae. (patentsencyclopedia.com)
and inoculate the axenic culture in a culture solution inside the PBR to culture microalgae. (patentsencyclopedia.com)
[0001] The present invention relates to an axenic inoculation system for microalgae using a triiodide resin (TR) tube of a triiodide resin, and a method for culturing an axenic culture. (patentsencyclopedia.com)
More particularly, a TR tube is fabricated using a triiodide resin and attached on an upper portion of a photobioreactor (PBR), and used for passing a culture solution containing microalgae, pure liquid and air through the TR tube. (patentsencyclopedia.com)
This project aims to bridge this gap, creating a scalable single-use photobioreactor for the production of axenic microalgae capable of producing high value products. (edu.au)

Axenic cultures of microorganisms are typically prepared by subculture of an existing mixed culture. (wikipedia.org)
One standard approach with microorganisms is to spread a sample of the culture onto an agar plate, and to incubate this for a fixed period of time. (wikipedia.org)
For example, a throat culture is taken by scraping the lining of tissue in the back of the throat and blotting the sample into a medium to be able to screen for harmful microorganisms, such as Streptococcus pyogenes , the causative agent of strep throat. (wikipedia.org)
It is often essential to isolate a pure culture of microorganisms. (wikipedia.org)
Microbial culture collections focus on the acquisition, authentication, production, preservation, catalogueing and distribution of viable cultures of standard reference microorganisms , cell lines and other materials for research in microbial systematics . (wikipedia.org)

To date, nine anaerobic fungal genera have been described, with further novel taxonomic groupings known to exist based on culture-independent molecular surveys. (frontiersin.org)
Lichen growth forms cannot be recapitulated in the laboratory by culturing the plant and fungal partners together. (sciencemag.org)

Selection of those cultures consisting solely of the desired organism produces the axenic culture. (wikipedia.org)
A total of 15 isolates of Giardia intestinalis, the first axenic cultures of this organism to be described from Germany, were established in Bonn from faecal cysts obtained from human and animal stool specimens. (nih.gov)
Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both. (wikipedia.org)
A pure culture may originate from a single cell or single organism, in which case the cells are genetic clones of one another. (wikipedia.org)

The work presented herein focused on determining axenic conditions for culturing and growing insect (epimastigote) forms of T. vivax and prompting their differentiation into metacyclic forms that are infectious for the mammalian host. (plos.org)
The medium used and other cultural conditions were, found to influence both the type of development and the longevity of cultured protoplasts. (ubc.ca)
However, a major impediment to validating this model is a poor understanding of the actual nutrient conditions encountered by M. oryzae during infection, what nutrients can be acquired from the host, and how closely axenic growth in synthetic minimal media mimics the nutrient conditions of the plant. (esiservizi.com)
Bacterial additives that consistently enhance rotifer growth under synxenic culture conditions 2. (springer.com)
Here, we describe modified ACCM and culture conditions that support improved growth of C. burnetii and their use in genetic transformation and pathogen isolation from tissue samples. (asm.org)
A microbiological culture , or microbial culture , is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. (wikipedia.org)
A total of 21 out of 79 amoeba peptidase genes analysed were found to be significantly expressed under standard axenic culture conditions whereas the remaining are not expressed or at very low levels only. (biomedcentral.com)
Under standard culture conditions or upon heat-stress only a relatively small number of these genes is significantly expressed and only very few variations become apparent between various clinical E. histolytica isolates, calling into question the importance of these enzymes in E. histolytica pathogenicity. (biomedcentral.com)
Appropriate culture conditions, media, and the effect of hormones on growth and development were studied. (biomedcentral.com)
Although culturing plant tissues and organs under axenic conditions was first established and profitably employed in bryophytes, especially mosses [ 13 ], bryophytes did not retain for long their rightful place as a highly favored research object. (biomedcentral.com)

A completely axenic C. burnetii genetic transformation system was developed using ACCM-2 that allowed isolation of transformants in about 2 1/2 weeks. (asm.org)

[4] [5] Culture collection are also repositories of type strains . (wikipedia.org)

In any published description of a culture, one must make every attempt to publish the characterization or history of the culture. (sivb.org)
Mixed cultures, co-cultures and pure (axenic) cultures and conventional and molecular procedures for their characterization and improvement. (unibo.it)

Development of media formulations permitting axenic culture of marine pathogens has enabled scientists to study the growth of parasites, their virulence factors and host-parasite interactions. (thefreedictionary.com)
2006). Even HeLa cells cultured with this parasite have been reported to secrete some cytokines (Chang et al. (scielo.br)
Host cell cultures were exposed to a parasite:BOECS cell ratio of 5:1 for 12 h in 37ºC ( Fig. 1C, D ). Cells were equilibrated in incubation medium for 5 min at 37ºC (5% CO 2 ) prior to parasite addition. (scielo.br)

The axenic cultures were successfully subcultured to fresh medium. (ubc.ca)
NMII was successfully cultured in ACCM-2 when medium was inoculated with as little as 10 genome equivalents contained in tissue homogenates from infected SCID mice. (asm.org)

1978. Method for the simultaneous establishment of many axenic cultures of Paramecium . (springer.com)

Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 mL of fresh ATCC medium 712 in a T-25 tissue culture flask or plastic 16 x 125 mm screw-capped test tube. (atcc.org)
Molecular detection of Coxiella burnetii in heart valve tissue from patients with culture-negative infective endocarditis. (pubfacts.com)
Here, we examined the diagnostic utility of Q fever polymerase chain reaction (PCR) of formalin-fixed heart valve tissue from patients with blood culture-negative IE who underwent heart valve surgery. (pubfacts.com)
Clonal propagation through tissue culture offers an alternative approach to vegetative propagation. (thefreelibrary.com)
In tissue culture, cytokinins play an important role as they promote cell division and develop meristematic centers leading to the formation of organs, mainly shoots (Peeters et al. (thefreelibrary.com)
There was, however, a significant difference in the average cell size, with diameters ranging from 9.30 +/- 0.184 to 11.11 +/- 0.22 microns and from 9.17 +/- 0.05 to 11.25 +/- 0.24 microns for cells growing in Excell 401 serum-free medium in spinner flask cultures and in TNM-FH medium supplemented with 10% FBS in tissue flask cultures, respectively. (biomedsearch.com)

Coxiella burnetii is a common cause of blood culture-negative infective endocarditis (IE). (pubfacts.com)

We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. (hindawi.com)
In 1988, our group reported an in-house developed axenic culture medium, PEHPS [ 14 ]. (hindawi.com)
The axenic culture medium 199 supplemented by 5% of calf newborn serum was used. (thefreedictionary.com)
About 100 [micro]l of the culture medium with 105 amastigotes from axenic culture with or without a serial drug dilution was seeded in 96-well microtiter plates. (thefreedictionary.com)
If the protoplasts isolated from either resistant or susceptible varieties, or both, were burst when incubated with exudate from axenic cultures or spore germination medium this would constitute evidence in support of the toxin theory. (ubc.ca)
The search for toxic substances in both axenic culture exudate and spore germination medium yielded negative results. (ubc.ca)
Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal slant for medium 2154). (atcc.org)
KEYWORDS: biomass production, Culture medium, Dunaliella sp. (bioone.org)
For the purpose of gelling the microbial culture, the medium of agarose gel ( agar ) is used. (wikipedia.org)

Axenic culture is an important tool for the study of symbiotic and parasitic organisms in a controlled environment. (wikipedia.org)

A cell line arises from a primary culture at the time of the first successful subculture. (sivb.org)
Growth studies are carried through two subculture generations and cell growth is plotted as a logarithmic function of time in culture. (sigmaaldrich.com)

1:800) and 7 minor criteria (positive blood cultures, fever, previous heart disease, arterial embolism, positive results on serologic examination for endocarditis bacterial pathogens, immunologic disorders, and atypical but compatible findings on echocardiography) ( 12 ). (cdc.gov)

The aim of this study was to determine whether T. foetus and T. vaginalis stimulated the release of the cytokine interleukin (IL)-10 from cultured bovine epithelial cells. (scielo.br)
To characterise the inflammatory response induced by these parasites, primary cultures of bovine oviduct epithelial cells were exposed to either T. vaginalis or T. foetus. (scielo.br)

A morphological description must await achieving axenic culture , but the partial sequence of the SSUrDNA has been given the GenBank accession numbers AF228362 and AF228363 for the V1, and V2 and V3 regions of the SSUrDNA, respectively. (thefreedictionary.com)

The recent description of a method for axenic (host cell-free) propagation of C. burnetii is a significant technological advance that will facilitate biochemical and genetic studies of the pathogen ( 22 ). (asm.org)

Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. (hindawi.com)
To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters. (hindawi.com)
This unit includes protocols for culturing worms on solid and axenic media and acute and chronic exposure parameters for Parkinson's disease-associated toxins and hemin chloride. (currentprotocols.com)
Various specific recipes for algal culture media are described by Vonshak (1986). (fao.org)
The complexity and cost of the above culture media often excludes their use for large-scale culture operations. (fao.org)
Anaerobic agents are the most difficult to detect from oxygen-containing environments and, therefore, it is critical that anaerobic collection culture media, anaerobic chambers, and powerful microbial identification technologies be utilized to ensure accurate results. (criver.com)
It is a common supplement for cell culture media. (sigmaaldrich.com)
FBS remains a popular media supplement because it provides a wide array of functions in cell culture. (sigmaaldrich.com)

All 62 blood culture-negative endocarditis cases were tested by serologic or molecular methods or both. (cdc.gov)
Our data underline the high prevalence of infective endocarditis caused by Bartonella quintana in northern Africa and the role of serologic and molecular tools for the diagnosis of blood culture-negative endocarditis. (cdc.gov)
Microbial cultures are foundational and basic diagnostic methods used extensively as a research tool in molecular biology . (wikipedia.org)
Traditional culture-based studies in the past have documented the trophic tendencies of various mixotrophs [ 10 - 13 ], but have not provided details on the molecular processes occurring in the organisms. (biomedcentral.com)
To clarify the molecular bases of this metabolic feature, we investigated light-driven lipid biosynthesis in Nannochloropsis gaditana cultures combining the analysis of photosynthetic functionality with transcriptomic, lipidomic and metabolomic approaches. (plantphysiol.org)

FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture. (sigmaaldrich.com)

The availability of the complete genome sequence of the nematode C. elegans , coupled with the worm's size, growth rate, ease of culturing, and the realization that basic biological mechanisms and disease processes between worms and humans are highly conserved, makes this genetically tractable model a remarkable opportunity to dissect and identify in vivo the cellular processes involved in toxin‐induced cell dysregulation and death. (currentprotocols.com)

Microbial cultures. (uv.es)
Conservation of microbial cultures. (unibo.it)

The data indicate that genetically based metabolic differences may determine how rapidly G. intestinalis isolates can grow in axenic culture. (nih.gov)

Nitrogen flow in a chemostat culture of the rotifer Brachionus plicatilis . (springer.com)

The role played by specific probiotic candidates is still indistinct and further research is required to ascertain their significance in rotifer culture. (springer.com)
In this study, euryhaline rotifer Brachionus rotundiformis (SS-type) was used to evaluate the effect of two commercial probiotic products on rotifer density, reproductive parameters and unionized ammonia concentration for a twelve-day culture period. (springer.com)

Ideally, cultured parasites should present the same growth and virulence characteristics as their counterparts in their natural habitat [ 8 ]. (hindawi.com)

The results in Chapter IV are an extension and modification of those in Chapter III and describe the axenic culture of poplar rust for the first time. (ubc.ca)
This term can also be used to describe agents which contaminate cell cultures. (sivb.org)

If culture was positive, the strain was identified. (cdc.gov)

The supernatants of cultures with parasites or without (controls) were collected and cytokine levels were quantified with specific kits for measuring IL-10 (Becton Dickinson, Franklin Lakes, NJ, USA), which were used according to the manufacturers' instructions. (scielo.br)

Most cases of blood culture-neg- using the modified Duke criteria, which includes 3 major ative endocarditis have been thought to be caused by pre- criteria (blood cultures typical of infective endocarditis, vious antimicrobial therapy. (cdc.gov)

Integrate a source of irradiance, optimised gas (CO2/O2) exchange and temperature control into GE Healthcare's Xcellerex bioreactor platform, set up a flexible sensor array for monitoring of the culture. (edu.au)

When available, cardiac tools for the diagnosis of blood culture-negative endo- valves and serum samples were stored to perform retro- carditis. (cdc.gov)
How is Fetal Bovine Serum, Product F2442, cell culture tested? (sigmaaldrich.com)
Hybridoma -tested serum has been shown to support mouse myeloma cells and hybridoma cells in culture. (sigmaaldrich.com)

Cattle infectious subspecies in axenic asynchronous culture. (lshtm.ac.uk)
Charles River offers comprehensive, accurate, and sensitive detection of infectious agents using both aerobic and anaerobic cultures of samples obtained from the isolator environment, individual cages, and directly from colony animals. (criver.com)

In a re-association experiment, cells from the heterotrophic culture were added to an axenic culture of the Anabaena sp. (thefreedictionary.com)
5. The rate of amino acid oxidation during axenic growth of the myxamoebae is decreased when the cells are supplied with glucose. (portlandpress.com)
Harvest cells from a culture that is at or near peak density by centrifugation at 700-800 x g for 5 min. (atcc.org)
For access to these features we have used the flow cytometry and fluorescence microscopy with cultures in the stationary phase and adjusted to a density of 10(6) cells /mL. (bvsalud.org)
There is hardly a field of biological investigation in which culturing of such cells is not employed. (sivb.org)
Cells, or cultures derived from them, which will grow, survive, or maintain function only when attached to a surface such as glass or plastic. (sivb.org)
The percentage of cells plated (seeded, inoculated) which attach to the surface of the culture vessel within a specified period of time. (sivb.org)
An axenic culture may include the purposeful cocultivation of different types of cells, tissues or organisms. (sivb.org)
In cell cultures, the cells are no longer organized into tissues. (sivb.org)
The term cell line implies that cultures from it consist of lineages of cells originally present in the primary culture. (sivb.org)
Batch culture experiments without the flagellate demonstrated that the elongation of C. acidovorans PX54 cells was dependent on their growth rate. (asm.org)
Concentrations of cells in phytoplankton cultures are generally higher than those found in nature. (fao.org)

Here we show that curcumin , a polyphenolic organic molecule derived from turmeric, inhibits chloroquine-resistant Plasmodium falciparum growth in culture in a dose dependent manner with an IC(50) of approximately 5 microM. (thenakedscientists.com)

More particularly, a TR tube is fabricated using a triiodide resin and a method for culturing an axenic culture. (patentsencyclopedia.com)

The Effect of Initial Cell and Nutrient Concentrations on the Growth and Biomass Production of Outdoor Cultures of Dunaliella sp. (bioone.org)
The final concentrations of non-axenic outdoor mass cultures of Dunaliella sp. (bioone.org)

Shell-vial culture using the JNSP protocol from January 1996 to September 2003. (asm.org)

There are several types of bacterial culture methods that are selected based on the agent being cultured and the downstream use. (wikipedia.org)
Improved Methods for the Axenic Culture of Puccinia recondita f. sp. (apsnet.org)

An Investigation on principle biochemical components, photosynthetic pigments, nucleic acid and enzymatic activities of axenic culture of Scytonema sp. (nepjol.info)

Moreover, skin microbiome studies have heavily relied on 16S rRNA sequencing data in the absence of bacterial culture. (bioportfolio.com)

Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. (hindawi.com)

An E. festucae Δ bemA mutant was defective in hyphal morphogenesis and growth in culture and in planta . (pnas.org)

This confusion is especially acute in the field of vertebrate, invertebrate and plant cell culture. (sivb.org)

Incubate the culture at 50-100 µEinsteins/m 2 /s irradiance at 25°C. Maintain under a 14/10h light-dark photoperiod. (atcc.org)

Organisms16

Diseases4

Chemicals and Drugs10

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Psychiatry and Psychology1

Phenomena and Processes2

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture3

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Leishmania major parasite stage-dependent host cell invasion and immune evasion. (1/14)

Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota. (2/14)

The genetic toolbox for Leishmania parasites. (3/14)

Epidemiologic consequences of microvariation in Mycobacterium tuberculosis. (4/14)

Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi. (5/14)

Targeting host syntaxin-5 preferentially blocks Leishmania parasitophorous vacuole development in infected cells and limits experimental Leishmania infections. (6/14)

Antileishmanial activity of warifteine: a bisbenzylisoquinoline alkaloid isolated from Cissampelos sympodialis Eichl. (Menispermaceae). (7/14)

The response of dark septate endophytes (DSE) to heavy metals in pure culture. (8/14)

Axenic Culture

Claviceps

Peptones

Culture Media

Myxomycetes

Entamoeba histolytica

Eukaryota

Biodegradation, Environmental

Phylogeny

DNA, Ribosomal

Molecular Sequence Data

RNA, Ribosomal, 16S

Nitrogen

Starvation

Microalgae

Iodides

Photobioreactors

Patents as Topic

Biofuels

Chlorophyta

Nitrate Reductase (NAD(P)H)

Cladocera

Lysobacter

Hydrocarbons

Chara

Tritrichomonas foetus

Tritrichomonas

Protozoan Infections, Animal

Trichomonas

Protozoan Infections

Trichomonadida

Trichomonas vaginalis

Commerce

Taxes

Prescription Fees

Philately

Great Britain

Germany

Medicine, Arabic

Western Australia

Telefacsimile

Genome, Mitochondrial

Sheep

Agricultural Irrigation

DNA, Mitochondrial

Problem Solving

Skin Manifestations

Bacteria

Polymerase Chain Reaction

Leishmania major parasite stage-dependent host cell invasion and immune evasion. (1/14)

Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota. (2/14)

The genetic toolbox for Leishmania parasites. (3/14)

Epidemiologic consequences of microvariation in Mycobacterium tuberculosis. (4/14)

Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi. (5/14)

Targeting host syntaxin-5 preferentially blocks Leishmania parasitophorous vacuole development in infected cells and limits experimental Leishmania infections. (6/14)

Antileishmanial activity of warifteine: a bisbenzylisoquinoline alkaloid isolated from Cissampelos sympodialis Eichl. (Menispermaceae). (7/14)

The response of dark septate endophytes (DSE) to heavy metals in pure culture. (8/14)

D in axenic cultures under conditions of nitrogen starvation [13,14], and at | esiservizi.com

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