Culture of an isolated organism free from any other associating or contaminating organisms.
A genus of ascomycetous fungi, family Clavicipitaceae, order Hypocreales, parasitic on various grasses (POACEAE). The sclerotia contain several toxic alkaloids. Claviceps purpurea on rye causes ergotism.
Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A division of organisms that exist vegetatively as complex mobile plasmodia, reproduce by means of spores, and have complex life cycles. They are now classed as protozoa but formerly were considered fungi.
A species of parasitic protozoa causing ENTAMOEBIASIS and amebic dysentery (DYSENTERY, AMEBIC). Characteristics include a single nucleus containing a small central karyosome and peripheral chromatin that is finely and regularly beaded.
One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
The relationships of groups of organisms as reflected by their genetic makeup.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
Individuals enrolled in a school or formal educational program.
The teaching staff and members of the administrative staff having academic rank in a medical school.
The teaching staff and members of the administrative staff having academic rank in an educational institution.
Individuals enrolled in a school of medicine or a formal educational program in medicine.
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
A biologic alkylating agent that exerts its cytotoxic effects by forming DNA ADDUCTS and DNA interstrand crosslinks, thereby inhibiting rapidly proliferating cells. The hydrochloride is an antineoplastic agent used to treat HODGKIN DISEASE and LYMPHOMA.
Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)

Leishmania major parasite stage-dependent host cell invasion and immune evasion. (1/14)

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Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota. (2/14)

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The genetic toolbox for Leishmania parasites. (3/14)

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Epidemiologic consequences of microvariation in Mycobacterium tuberculosis. (4/14)

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Apoptotic-like activity of staurosporine in axenic cultures of Trypanosoma evansi. (5/14)

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Targeting host syntaxin-5 preferentially blocks Leishmania parasitophorous vacuole development in infected cells and limits experimental Leishmania infections. (6/14)

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Antileishmanial activity of warifteine: a bisbenzylisoquinoline alkaloid isolated from Cissampelos sympodialis Eichl. (Menispermaceae). (7/14)

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The response of dark septate endophytes (DSE) to heavy metals in pure culture. (8/14)

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The earliest axenic cultures were of bacteria or unicellular eukaryotes, but axenic cultures of many multicellular organisms ... identifying cultures that are no longer axenic.[citation needed] As axenic cultures are derived from very few organisms, or ... Axenic cultures of microorganisms are typically prepared by subculture of an existing mixed culture. This may involve use of a ... Axenic cultures are usually checked routinely to ensure that they remain axenic. One standard approach with microorganisms is ...
Axenic culture "Germ Free Mouse Facility". University of Michigan. Archived from the original on 30 October 2015. Reyniers JA ( ... There are two main methods for yielding gnotobiotic or axenic flies. One of them is to collect the eggs and dechorionate them. ... "Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions". Journal of Visualized Experiments (113 ...
Contaldo, N.; Bertaccini, A.; Paltrinieri, S.; Windsor, H.M.; Windsor, G.D. (2012). "Axenic culture of plant pathogenic ... used for bacteria that cannot be cultured). Phytoplasma taxonomy is complicated because the organisms cannot be cultured; ... Tissue culture can be used to produce healthy clones of phytoplasma-infected plants. Cryotherapy (i.e., the freezing of plant ... Since their discovery, phytoplasmas have resisted all attempts at in vitro culture in any cell-free medium; routine cultivation ...
In the 1990s a group of high school students in New Hampshire made progress on axenic culture from seed and were able to obtain ... "Axenic Seed Culture of Cypripedium arietinum" (PDF). Static1.squarespace.com. Retrieved 18 March 2022. Sokolski K. & Peter ... 1997). "Axenic Seed Culture and Micropropagation of Cypripedium reginae" Selbyana 18(2): 172-182. "Minnesota State Symbols". ... in Axenic Seed Culture, Bulletin of America Association for the Advancement of Sciences, Annual meeting, pp. A-112 AAAS Annual ...
"Axenic seed culture and micropropagation of Cypripedium reginae". Selbyana. 18 (2): 172-82. JSTOR 41760430. Chouard, P. (June ...
Gifford, Jr., Ernest M.; Brandon, Dorothy D. (1978). "Gametophytes of Botrychium multifidum as Grown in Axenic Culture". ...
Gametophytes of Lycopodium obscurum as grown in axenic culture. Canadian Journal of Botany. 55. pp. 563-567. Hickey, R. James. ...
Brenner then received cultures of this strain from Dougherty. Over the decades since Nick's first axenic cultures of C. elegans ... Dougherty, Ellsworth C. (1959). "Introduction to Axenic Culture of Invertebrate Metazoa: A Goal". Annals of the New York ... produced the axenic culture of it, and brought it to Dougherty's lab. It was a remarkable journey in itself transporting the ... an objective was to determine the nature of undefined factors of Rb and Cb required for axenic culture of Caenorhabditis ...
Berch SM, Castellano MA (1986). "Sporulation of Endogone pisiformis in axenic and monaxenic culture". Mycologia. 78 (2): 292- ... 1990). "Taxonomy, culture, and mycorrhizal associations of some zygosporic Endogonaceae". Mycological Research. 94 (2): 173-178 ... pisiformis can be made to sporulate in test tube culture when grown with conifer seedlings. Endogone was first circumscribed by ...
The mycelium of Tylopilus felleus can be grown in axenic culture, on agar containing growth medium. The fungus can form fruit ... 1974). "Fruitbody formation by Tylopilus fellus in axenic culture". Mycologia. 66 (1): 197-202. doi:10.2307/3758474. JSTOR ... There are few Boletaceae species known to fruit in culture since ectomycorrhizal fungi tend to not fruit when separated from ...
This work was important in the development of axenic culture methods. C. briggsae can often be found in compost, garden beds, ...
In 2005, a monoclonal culture of the strain was isolated. The axenic strain is available from the Center for Culture of Marine ... The antibiotic susceptibility was determined using a single strain of M. pusilla with the purpose to produce axenic cultures to ... Cottrell, Matthew T.; Suttle, Curtis A. (1 June 1993). "Production of Axenic Cultures of Micromonas Pusilla (Prasinophyceae) ... This strain has been continuously cultured for two decades and is available from the Roscoff Culture Collection. ...
The tips of the hyphae were used to obtain axenic cultures; the fungus can grow on a variety of media commonly used to grow ... Escobar grew cultures of the fungus by placing fresh fruit bodies on agar containing growth medium with an extract of horse ... Experimental observations on the development of the fruit body, based on the growth on the fungus in pure culture, suggest that ... Mycologist Dennis McCabe studied the development of the fruit body using specimens grown in pure culture. Starting out as a ...
Baker, KL; Beneke, ES; Hooper, GR; Fields, WG (1977). "Host Range and Axenic Culture of the Mycoparasite Syncephalis sphaerica ...
I. Isoenzyme and Soluble Protein Patterns of Axenic Cultures of Symbiodinium microadriaticum". Proceedings of the Royal Society ... In culture, the discarded walls accumulate at the bottom of the culture vessel. It is not known what becomes of the walls from ... Culturing is a selective process, and many Symbiodinium isolates growing on artificial media are not typical of the species ... All cultured isolates (i.e. strains) are capable of phenotypic adjustment in their capacity for light harvesting (i.e. ...
Specific-pathogen-free (SPF) Axenic culture Basic M, Bleich A (June 2019). "Gnotobiotics: Past, present and future". Laboratory ... Gnotobiotic plants are plants that are either grown without microorganisms present (aseptic, axenic, or sterile) or grown in ... Hale MG, Lindsey DL, Hameed KM (1973). "Gnotobiotic Culture of Plants and Related Research". Botanical Review. 39 (3): 261-273 ...
Therefore, axenic cultures of trichomycetes are highly valuable for obtaining pure DNA samples. As a result, the phylogenetic ... to be obtained in axenic culture, allowing for detailed studies of its nutritional requirements, cell wall composition, and ... Amoebidium appalachense was also obtained in axenic culture, and subsequent molecular analyses supported its relationship with ... Culture and nutrition of Amoebidium parasiticum. American Journal of Botany 49: 193-199. Trotter, M. J., and Whisler, H. C. ...
These animal cells are added to a specified axenic growth medium for culturing. At the same time, and xenic culture is also ... Several types of animal cells have been used in B. mandrillaris culturing including rat glioma cells, human lung cells, and ... The amoeba cannot be cultured on an agar plate coated with E. coli because, unlike Naegleria or Acanthamoeba, Balamuthia ... Balamuthia , Parasites , CDC for images: Cyst of B. mandrillaris and Trophozoite of B. mandrillaris in culture. Credit: DPDx ( ...
Whittier, P. (1981). "Spore Germination and Young Gametophyte Development of Botrychium and Ophioglossum in Axenic Culture". ...
Axenic culture of amoebae was achieved through selection of mutants capable of axenic growth. Under conditions of starvation or ... Like amoebae, the plasmodium can consume whole microbes, but also readily grows axenically in liquid cultures, nutrient agar ... McCullough, Claire (1978). "Genetic Factors Determining the Growth of Physarum polycephalum Amoebae in Axenic Medium" (PDF). ...
These cultures have strain numbers preceded by BEA. Non-axenic cultures are marked with "B" at the end of the strain number. ... BEA has been member of the European Culture Collection Organization (ECCO) since 2001 and of the World Federation for Culture ... "Spanish Type Culture Collection (University of Valencia)" (in Spanish). Retrieved 26 December 2011. "European Culture ... gDNA "à la carte": BEA is able to extract genomic DNA from microalgae cultures from a wide range of volumes. Normally kits (i.e ...
Goldstein, Solomon; Belsky, Melvin (1964). "Axenic Culture Studies of a New Marine Phycomycete Possessing an Unusual Type of ... Thraustochytrids are currently cultured for use in fish feed and production of dietary supplements for humans and animals. In ... Role of Culture Conditions, Energy Sources, and Inhibitors". Journal of Bacteriology. 93 (4): 1281-1288. doi:10.1128/jb.93.4. ... There have been cases of thraustochytrids being cultured from algal surfaces but only in low numbers. Notably, in a case of ...
"Metabolites from the Fungal Endophyte Aspergillus austroafricanus in Axenic Culture and in Fungal-Bacterial Mixed Cultures". ...
Production of DMS from dissolved DMSP in axenic cultures of the marine phytoplankton species Phaeocystis sp., Mar. Ecol. Prog. ... not in culture), P. cordata, and P. rex. Three species (P. globosa, P. pouchetii, and P. antarctica) are associated with bloom ...
... in axenic culture on defined media. Retrieved from https://ir.library.oregonstate.edu/xmlui/handle/1957/44218 Archibald, P. A ... doi:10.1007/bf00940436 Ahmadjian, V. (1967). A Guide to the Algae Occurring as Lichen Symbionts: Isolation, Culture, Cultural ...
... multiseries cultures which were completely axenic (bacteria-free), produce less domoic acid than P. multiseries cultures which ... "Enhancement of domoic acid production by reintroducing bacteria to axenic cultures of the diatom Pseudo-nitzschia multiseries ... For example, in a mixed batch culture the target species may die or have reduced growth rates due to competition for nutrients ... All methods of detecting phycotoxins involve extraction of the candidate toxin from a phytoplankton culture; therefore, it's ...
... they were able to grow the organism in axenic cultures from single-spore isolates of C. geaster. Until the one fungus, one name ... Finally, only Japanese specimens can be grown in culture-the spores of Texan material have not been successfully germinated on ...
... expansum can be identified by its morphological characteristics and secondary metabolites in fruit or in axenic culture. The ...
"Effect of ammonium ions on egg hatching and second-stage juveniles of Meloidogyne incognita in axenic tomato root culture". ...
Hill, S.T. (1965). "Axenic culture of the foreign grain beetle, Ahasverus advena (Waltl) (Coleoptera, Silvanidae), and the role ...
It is often essential to isolate a pure culture of microorganisms. A pure (or axenic) culture is a population of cells or ... Colony-forming unit Blood culture Microbial dark matter Microbial Food Cultures Screening cultures Sputum culture Synchronous ... Stab cultures are most commonly used for short-term storage or shipment of cultures. Microbial culture collections focus on the ... it is necessary to preserve it in a viable state for further study and use in cultures called stock cultures. These cultures ...
The culture is kept under lighting with temperature and pH value held constant. The culture medium-often a minimal medium- ... Algae bioreactor Bioreactor Molecular farming Photobioreactor Physcomitrella patens Hohe, A.; Reski, R. (2005). "From axenic ... At this point the culture has to be diluted with fresh medium if it is intended for further use. According to the intended ... Once the density of the culture has reached a certain threshold, the lack of nutrients and the increasing concentration of ...
in axenic medium" (PDF). Zoological Science. 18 (3): 381-7. doi:10.2108/zsj.18.381. Archived from the original (PDF) on 2017-10 ... Successful culturing of opalines in artificial media for periods of 1 month or more has been reported. This technique will aid ...
Symbiotic Symbiotaphrina species can be isolated in axenic culture by aseptically dissecting beetle guts, spreading them onto ... Shen, S.K.; Dowd, P.F. (1991). "Detoxification spectrum of the cigarette beetle symbiont Symbiotaphrina kochii in culture". ...
However, in 2009 scientists reported a technique allowing the Q-fever pathogen Coxiella burnetii to grow in an axenic culture ...
Efforts to culture the bacteria in vitro remain unsuccessful. Because the organism is an obligate intracellular parasite, it ... The growth conditions needed for Mycobacterium leprae are known, but an exact axenic medium to support the growth of ... Mycobacterium leprae's inability to be grown in axenic media indicates its reliance on nutrients and intermediates from its ... as well as its inability to be cultured in the laboratory. Comparing the genome sequence of Mycobacterium leprae with that of ...
This is similar to how viruses cannot grow in axenic culture: viruses must be living to replicate, so the culture is not a ... The microorganism must be isolated from a diseased organism and grown in pure culture. The cultured microorganism should cause ... be grown in pure culture. Viruses also require host cells to grow and reproduce and therefore cannot be grown in pure cultures ... Some pathogens cannot be cultured in the lab, and some pathogens only cause disease in humans. In summary, an infectious agent ...
He grew over 150 trichomycete isolates in axenic culture, a critical step for experimental studies on nutritional modes and ... "Culture collection". www.nhm.ku.edu. Archived from the original on 2004-08-12. (Articles with short description, Short ...
Stool culture was considered by some researchers to be the most reliable technique, but a recent study found stool culture only ... Lanuza MD, Carbajal JA, Villar J, Mir A, Borras R (1999). "Soluble-protein and antigenic heterogeneity in axenic Blastocystis ... Protozoal culture is unavailable in most countries due to the cost and lack of trained staff able to perform protozoal culture ... Stool culture: Culturing has been shown to be a more reliable method of identifying infection. In 2006, researchers reported ...
Grown in axenic culture in the laboratory, the fungus mycelium has been shown to degrade (in addition to lignin and cellulose) ... a potent antifungal metabolite first isolated from submerged cultures of Pterula species in 1997. The antifungal activity of ...
Allen, Rey; Rittmann, Bruce E.; Curtiss, Roy (2019). "Axenic Biofilm Formation and Aggregation by Synechocystis sp. Strain PCC ... Villareal, Tracy A. (1990). "Laboratory Culture and Preliminary Characterization of the Nitrogen-Fixing Rhizosolenia-Richelia ...
... penetrates the rhizoids of liverworts and may form a type of symbiosis with them, but in axenic culture tests, L. ... Bresinsky, A. & Schötz, A. (2006). "Behaviour in cultures and habitat requirements of species within the genera Loreleia and ...
In unialgal cultures, Ectocarpus are described to have a branched appearance, in contrast, axenic cultures show Ectocarpus ... Cultured specimens in the laboratory tend to be fertile as early as 1-3 cm in length. ... A protocol has been established in culturing Ectocarpus in the laboratory using sporophyte filament as a starter, collected and ...
... of the host and grow in axenic culture, at least some species may be facultative symbionts. The "amoeb-" prefix refers to the ...
... axenic) cultures are isolated or available, i.e. not cultivated or can not be sustained in culture for more than a few serial ...
Furthermore, no species have been obtained in axenic culture, so observations are limited to the individuals present at the ...
... ignore species of morphologically variant bacteria that are unable to grow within axenic cultures. Winogradsky explained the ...
There, and during travels around the globe, he performed research on the axenic culture of nematodes, nematode parasites of ...
M-CMRL, a new axenic medium to replace indicator cell cultures for the isolation of all strains of M. hyorhinis. In Vitro Cell ... Isolation of mycoplasma from cell cultures by axenic techniques. In: Tully JG, Razin S, editors. Molecular and diagnostic ... Each culture plate was inoculated with 0.1 mL of vaccine. M-CMRL plates were incubated aerobically at 36°C, and SP-4 and DM-1 ... The cultures are grown in a sterile synthetic liquid medium devoid of enriched supplements such as serum. The filtrates ...
For some parasites, axenic cultures can be obtained for research purposes. They are not needed for diagnosis. ... This is a scanning electron micrograph (SEM) of an in vitro Giardia lamblia culture. This photograph contains both trophozoites ... This is a scanning electron micrograph (SEM) of an in vitro Giardia lamblia culture. This photograph contains both trophozoites ... E histolytica can be cultured for research and susceptibility purposes (especially for emetine susceptibility) but does not ...
Use of Antibiotics for Maintenance of Axenic Cultures of Amphidinium carterae for the Analysis of Translation. Marine Drugs 15( ... Use of Antibiotics for Maintenance of Axenic Cultures of Amphidinium carterae for the Analysis of Translation. Marine Drugs 15( ... nutrient-limited chemostat cultures and nutrient-enriched batch cultures. Mar Pollut Bull 104(1-2):34-43. ... The effects of elevated CO2 on the growth and toxicity of field populations and cultures of the saxitoxin-producing ...
Kaneda Y, Tanaka T, and Saw T. Effects of berberine, a plant alkaloid, on the growth of anaerobic protozoa in axenic culture. ... its cultured cells extract, and combined use of these extracts and glycyrrhizae radix extract in rats]. Yakugaku Zasshi 1993; ...
... species was the unicellular green alga Selenastrum capricornutum ATCC 22662 from laboratory cultures maintained under axenic ... A 3 day old culture of the alga in the exponential growth phase was used as inoculum for the test. The culture was grown in the ... Six replicate cultures of the culture medium control, solvent control and each concentration of Aquapel 364 were employed. The ... See standard culture medium. Test temperature:. 24.0 - 24.1 C. pH:. 7.4-7.5 at the start of the test. 9.1-9.7 at the end of the ...
Axenic Culture Preferred Concept UI. M0556796. Scope Note. Culture of an isolated organism free from any other associating or ... Culture of an isolated organism free from any other associating or contaminating organisms.. Entry Term(s). Axenic Cultures See ... Axenic Culture Preferred Term Term UI T789063. Date04/15/2011. LexicalTag NON. ThesaurusID NLM (2012). ... Axenic Cultures Term UI T790060. Date05/03/2011. LexicalTag NON. ThesaurusID NLM (2012). ...
In contrast, cell-dense cultures grown under optimal conditions appear more sensitive to exposure during extended incubation, ... In contrast, cell-dense cultures grown under optimal conditions appear more sensitive to exposure during extended incubation, ... Axenic Prochlorococcus sp. MED4 cultures (isolate source: Mediterranean Sea, South France) were grown on-site using Pro99 media ... axenic culture was added to NSW or Pro99 media, respectively, and preadapted to experimental conditions: 23°C and 10 μmol ...
Axenic , Agar. .strain-tags a#media-type { background: #363630; } .strain-tags a#media-type:before { border-color: transparent ... when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, ... FAQs: Living Algal Strains FAQs: Culture Maintenance Preparation of Living Algal Strains For Orders. For agar cultures, strains ... One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no ...
1970; Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J 119:171-174 ... Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria Rosmarie Rippka, Josette Deruelles, John ...
Culture system. Axenic Handling procedure. Storage and Culture Initiation Frozen ampules packed in dry ice should either be ... Culture maintenance. *Vigorously agitate a culture at or near peak density and aseptically transfer 0.1 ml to 0.2 ml to a fresh ... To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just sufficiently to ... For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid ...
However, this strain has strong interactions with other bacteria, making it impossible to obtain axenic cultures for sequencing ... However, this strain has strong interactions with other bacteria, making it impossible to obtain axenic cultures for sequencing ... However, this strain has strong interactions with other bacteria, making it impossible to obtain axenic cultures for sequencing ... However, this strain has strong interactions with other bacteria, making it impossible to obtain axenic cultures for sequencing ...
Axenic culture media for E. histolytica:. TYI-S-33 (12): Dissolve in this order in 600 ml deionized or glass distilled water:. ... monoxenic and axenic E. dispar SAW760, axenic E. invadens IP-1, E. moshkovskii Laredo and FIC, and E. terrapinae M) with ... dispar is that they are refractory to axenization by the classic technique for obtaining axenic E. histolytica cultures. One ... To complete TYI-S-33 medium for use in axenic cultivation add 2.0 ml of vitamin mix #18 and 10-15 ml of heat inactivated adult ...
These passive systems will be created by co-encapsulating axenic bacterial cultures with a slow release compound (SRC) in ... of Passive and Sustainable Cometabolic Systems to Treat Complex Contaminant Mixtures by Encapsulating Microbial Cultures and ...
Exponential phase axenic cultures were aliquoted for further Tosedostat inhibitor database experiments. Assays for Algicidal ... a total volume of 25 L culture medium having the same composition as the pre-culture. The culture broth was centrifuged at ... The pre-culture (incubated at 28C for 1 day in an orbital incubator set to 150 rpm) was used to inoculate (5% v/v) ... All Cultures were maintained in f/2 medium (without silicate) prepared with natural seawater [34] at 201C under a 1212 h ...
Evaluation of antioxidant and anticancer activity of crude extract and different fractions of Chlorella vulgaris axenic culture ... The effect of recycling culture medium after harvesting of Chlorella vulgaris biomass by flocculating bacteria on microalgal ...
2005). Both Bs and Fg conidia were harvested from 10 to 14-day-old axenic cultures with a sterile glass rod and suspended in ... 2001). Fusarium graminearum wild-type strain 1003 (teleomorph: Gibberella zeae) was cultured on Haarleen Agar (HA; 8 g malt ... Bipolaris sorokiniana culture KN2 was grown on complete medium at 25 °C and propagated as described in Kumar et al. ( ...
To obtain axenic cultures of the pathogen, fresh potato tubers of the susceptible variety Asante were cleaned under running tap ... After cooling, the media was dispensed into petri dishes and P. infestans isolates were maintained in culture. The cultures ... Inoculum was made by adding 5 mL of distilled water to the culture plates and gently scraping off mycelia using sterile blades ... The infected internodes were placed on co-culture medium and subsequently transferred to regeneration medium (Cuellar et al., ...
Gillespie on developing axenic culture media to support cell-free Rickettsia growth. ...
A11.671.501.234.60 Axenic Culture E5.240.124 E5.481.500.124 Axons A8.663.542.145 A8.675.542.145 A11.671.501.145 Batch Cell ... I1.880.853.100.450 Culture I1.880.143 I1.880.853.100 Culture Techniques E5.240 E5.481.500 Cystoscopy E4.800.250.180 E4.502. ... A8.186.211.730.317.578 Organ Culture Techniques E5.240.484 E5.481.500.484 Orphan Drug Production J1.576.318.600 J1.576.655.750. ... A11.671.501.145.750 Primary Cell Culture E5.240.249.500 E5.481.500.249.500 Proctoscopy E4.800.250.250.250.680 E4.502.250.250. ...
Reconstituted syntrophic cultures from axenic cultures immediately resumed syntrophic growth, and the calculated growth rates ... of these cultures were highest for cells which were inoculated from the axenic S. fumaroxidans cultures that exhibited the ... hungatei were studied in syntrophic propionate-oxidizing cultures and compared to the levels in axenic cultures of both ...
Axenic Culture Preferred Concept UI. M0556796. Scope Note. Culture of an isolated organism free from any other associating or ... Culture of an isolated organism free from any other associating or contaminating organisms.. Entry Term(s). Axenic Cultures See ... Axenic Culture Preferred Term Term UI T789063. Date04/15/2011. LexicalTag NON. ThesaurusID NLM (2012). ... Axenic Cultures Term UI T790060. Date05/03/2011. LexicalTag NON. ThesaurusID NLM (2012). ...
Axenic Culture MeSH DeCS ID:. 5995 Unique ID:. D005856 NLM Classification:. QY 58 ... Animal, Axenic. Animals, Axenic. Axenic Animal. Axenic Animals. Germ Free Life. Germfree Life. Gnotobiotic. Gnotobiotics. Life ... Axenic Animals - Narrower Concept UI. M0009204. Preferred term. Axenic Animals Entry term(s). Animal, Axenic Animals, Axenic ...
In axenic cultures, Ped. pentosaceus MITJ-10 produced more diacetyl (63.27 mg l(-1)) by adding glucose, yeast extract and CaCO3 ... In this study, apple juice was fermented using Hanseniaspora osmophila X25-5 in pure culture as well as mixed culture with ... Hanseniaspora uvarum UFLA CAF76 showed the best fermentation performance; hence it was selected to evaluate different culture ... Three series of 5-day submerged cultures with Pediococcus pentosaceus MITJ-10 and Lactobacillus acidophilus Hansen 1748 were ...
... isolated and cultured in axenic condition for research purposes.. Selected Publications. Mutalipassi, M., Mazzella, V., Schott ... Mutalipassi M, Di Natale M, Mazzella V, Zupo V. Automated culture of aquatic model organisms: Shrimp larvae husbandry for the ...
Axenic Culture Batch Cell Culture Techniques Cell Engineering Chemistry Techniques, Synthetic Chemoradiotherapy ... Primary Cell Culture Real-Time Polymerase Chain Reaction Solid-Phase Synthesis Techniques Therapy with Helminths Ultrasonic ...
Axenic culture of cardiac valves was positive for 9 samples. The growth of 2 coagulase-negative Staphylococcus, 2 Streptococcus ... Blood Cultures. Blood cultures identified 48 microorganisms (Table 2). Of the 22 Streptococcus spp. cultures, 5 Streptococcus ... Axenic Culture. Thirty-eight excised cardiac valves were examined. If macroscopic lesions of infective endocarditis were ... Blood Cultures. Either Castaneda Aer/Anaer (Bio-Rad, Marnes-La-Coquette, France) or broth for blood culture (Institut Pasteur ...
Current axenic algae cultivation systems lack robustness, are expensive and generally have marginal energy returns. Here it is ... 34] showed a volumetric productivity of 730 g m−3 day−1 for an axenic culture of Chlorella vulgaris grown in a 12:12 light and ... This value is remarkably high compared to other axenic culture systems and is a product of the very high volumetric ... Fatty acid content and profiles were consistent with previously reported axenic cultures of cyanobacteria. It should be noted ...
Scanning electron micrograph of dichotomously branched, subterranean gametophyte of Psilotum nudum grown in axenic culture. ...
For this reason, transformations that lead to the mineralization of organic materials by axenic cultures or microbial consortia ... may be exploited for the purposes of environmental remediation and waste treatment Axenic cultures or mixed populations of ... To each jar 2,3,4 or 5 % lactose was added, then the sample was inoculated with 1 ml of a culture of L. plantarum grown for 18 ... These doubling times have been measured as 1-2 days and 3-6 days respectively under optimal conditions in pure culture, and are ...
Axenic culture of selected tissue of Posidonia oceanica.. Aquatic Botany, 37 : 171-188. ... Cultures en milieu artificiel de Posidonia oceanica partir de graines.. Proceedings 2nd International Workshop on Posidonia ... Growth and devopment in culture of orthotropic rhizomes of Posidonia oceanica.. Aquatic Botany, 39 : 367-377. ... Analyse bibliographique sur la culture des phan rogames marines.. Posidonia newsletter 80, 3(1) : 2-67. ...
  • To complete TYI-S-33 medium for use in axenic cultivation add 2.0 ml of vitamin mix #18 and 10-15 ml of heat inactivated adult bovine serum to each 88 ml of TYI broth. (lshtm.ac.uk)
  • Current axenic algae cultivation systems lack robustness, are expensive and generally have marginal energy returns. (biomedcentral.com)
  • Present approaches typically focus on the axenic cultivation of a single strain, such as Spirulina , Nannochloropsis , Chlorella or Dunaliella . (biomedcentral.com)
  • E histolytica can be cultured for research and susceptibility purposes (especially for emetine susceptibility) but does not contribute to diagnostic purposes. (medscape.com)
  • In most cases 13 ml of TYI-S-33 per tube is the correct amount as inocula for axenic E. histolytica are generally small in established cultures. (lshtm.ac.uk)
  • One characteristic of what were once known as 'non-pathogenic E. histolytica ' isolates and are now known as E. dispar is that they are refractory to axenization by the classic technique for obtaining axenic E. histolytica cultures. (lshtm.ac.uk)
  • The cultures are grown in a sterile synthetic liquid medium devoid of enriched supplements such as serum. (cdc.gov)
  • The culture was grown in the medium, and under the environmental conditions, described for the test. (europa.eu)
  • In contrast, cell-dense cultures grown under optimal conditions appear more sensitive to exposure during extended incubation, likely as a result of increased rate of encounter between cyanobacteria and nanoparticles at high cell densities. (frontiersin.org)
  • 2. Evaluation of antioxidant and anticancer activity of crude extract and different fractions of Chlorella vulgaris axenic culture grown under various concentrations of copper ions. (nih.gov)
  • In order to extract the algicidal compounds, BS02 was prepared in distilled water from cultures grown on AC1 solid medium, was used to inoculate 1000 mL flasks, containing 500 mL AC1 liquid medium. (woofahs.com)
  • One set was tested by culture techniques for the presence of live organisms at the Mycoplasma Laboratory, Science Applications International Corporation, National Cancer Institute, Frederick Research and Development Center, Frederick, MD. A commercial nongovernment facility, Charles River Tektagen, tested the second set for mycoplasma DNA by polymerase chain reaction (PCR) assay. (cdc.gov)
  • Culture of an isolated organism free from any other associating or contaminating organisms. (nih.gov)
  • UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. (utex.org)
  • One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. (utex.org)
  • 2. Isolation and culture methods of algae. (nig.ac.jp)
  • The biphasic or soil-water culture method for growing algae and flagellata. (nig.ac.jp)
  • In Cultures and Collections of Algae, Eds. (nig.ac.jp)
  • Fresh- and salt-water forms of Spirulina platensis in axenic cultures. (limnology-journal.org)
  • I) On the pure culture of Spirulina platensis. (nig.ac.jp)
  • The test species was the unicellular green alga Selenastrum capricornutum ATCC 22662 from laboratory cultures maintained under axenic conditions. (europa.eu)
  • A 3 day old culture of the alga in the exponential growth phase was used as inoculum for the test. (europa.eu)
  • Growth characteristics of freshwater red tide alga, Peridinium based on axenic culture. (nig.ac.jp)
  • For agar cultures , strains are transferred to the surface of fresh agar slants in 20 mm diamter glass screw-cap tubes in preparation for shipment. (utex.org)
  • Agar and liquid cultures are packaged within a Styrofoam box and shipped the same day they are packaged. (utex.org)
  • Components and Strategies Bacterial Ethnicities BS02 was cultured in sea agar 2216E (pH 7.47.8) in 25C for 2448 h, and was preserved in ?80C in marine broth with 20% (v/v) glycerol. (woofahs.com)
  • Assays for Algicidal Activity The algicidal activity was carried out in 24-well plates (2 mL of cultures in the exponential growth phase were assigned to each well). (woofahs.com)
  • Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen. (atcc.org)
  • The extracted fractions or purified components dissolved in Dimethyl sulfoxide (DMSO) were added into cultures at different final concentrations in triplicate. (woofahs.com)
  • The utility of Wolbachia in control applications would be enhanced if the microbe could be experimentally manipulated by genetic engineering to express selectable markers, which in turn will be advanced by improving manipulation of Wolbachia in cell lines and expanding the diversity of Wolbachia strains that can be investigated in culture. (encyclopedia.pub)
  • 11. The effect of recycling culture medium after harvesting of Chlorella vulgaris biomass by flocculating bacteria on microalgal growth and the functionary mechanism. (nih.gov)
  • On the same time, we are building a Cyanobacteria bank, where a large variety of marine species are going to be identified, isolated and cultured in axenic condition for research purposes. (szn.it)
  • The vaccine, which is administered subcutaneously over an 18-month schedule, is derived from sterile, acellular filtrates of microaerophilic cultures of the avirulent, nonencapsulated V770-NP1-R strain of Bacillus anthracis . (cdc.gov)
  • It turns out that "axenic" flies-those raised in a sterile environment-leap, at the age of about 10 days, to a kind of metabolic middle age (30 days or so) and remain there until the end, which is itself delayed by about 20 percent compared to siblings raised under normal, non-sterile conditions. (nih.gov)
  • On each day of analysis, the sample preparation procedure was validated by fortifying triplicate 20 ml aliquots of culture medium with 50 ul of a 400 mg L-1 stock of Aquapel 364, prepared in acetone. (europa.eu)
  • This study was run with a culture medium and a solvent control together with a nominal Aquapel 364 concentration of 1.0 mg I-1. (europa.eu)
  • The test solution was prepared from this stock solution by addition of appropriate volumes to 1 L of culture medium and stirring thoroughly. (europa.eu)
  • A solvent control was similarly prepared by the addition of 0.1 ml of acetone to 1 litre of culture medium, to give a solvent concentration of 100 ul/L. The control consisted of culture medium only. (europa.eu)
  • All Cultures were maintained in f/2 medium (without silicate) prepared with natural seawater [34] at 201C under a 1212 h lightCdark cycle with a light intensity of 50 mmol photons m?2s?1. (woofahs.com)
  • The pre-culture (incubated at 28C for 1 day in an orbital incubator set to 150 rpm) was used to inoculate (5% v/v) a total volume of 25 L culture medium having the same composition as the pre-culture. (woofahs.com)
  • In contrast, E. coli and many familiar Gram-negative pathogens of humans classified as gamma-proteobacteria can be cultured in liquid medium and plated on solid media as free-living microbes. (encyclopedia.pub)
  • Establishment of synthetic culture medium [Junsuibaiyoho niyoru tansuiakashio Peridinium no zosyokutokusei no kaiseki-goseibaichi no kakuritsu]. (nig.ac.jp)
  • Laboratory culture and morphology of Colacium vesiculosum Ehrb. (nig.ac.jp)
  • Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. (utex.org)
  • After a stock culture is established, subcultures may be used for testing other conditions. (utex.org)
  • Competitive dominance of the cyanobacterium Microcystis aeruginosa in nutrient-rich culture conditions with special reference to dissolved inorganic carbon uptake. (limnology-journal.org)
  • Bartonella melophagi in blood of domestic sheep (Ovis aries) and sheep keds (Melophagus ovinus) from the southwestern US: Cultures, genetic characterization, and ecological connections. (cdc.gov)
  • To harvest algal biomass from a suspended culture, the culture is commonly concentrated via sedimentation or flocculation to around 1% (dry basis) then further concentration using centrifugation to 20% (dry basis) [ 16 , 17 ]. (biomedcentral.com)
  • Part I. Methods and culture media. (nig.ac.jp)
  • Culture media for Euglena gracilis. (nig.ac.jp)
  • For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. (atcc.org)
  • and (3) metabolites not produced in axenic animals. (who.int)
  • Coupling feeding activity, growth rates and molecular data shows dietetic needs of Ciona robusta (Ascidiacea, Phlebobranchia) in automatic culture plants. (szn.it)
  • Our data underline the high prevalence of infective endocarditis caused by Bartonella quintana in northern Africa and the role of serologic and molecular tools for the diagnosis of blood culture-negative endocarditis. (cdc.gov)
  • Mycoplasma contamination is considered to be a potential problem for vaccines that are produced in cell cultures. (cdc.gov)
  • In this study, apple juice was fermented using Hanseniaspora osmophila X25-5 in pure culture as well as mixed culture with Torulaspora quercuum X24-4, which was inoculated simultaneously or sequentially. (bvsalud.org)
  • The rate of blood culture-negative endocarditis in Algeria is as high as 76% ( 2 ), which leads to difficulty in antimicrobial treatment. (cdc.gov)
  • Collection and mass culture [Shiryo no saisyu to baiyo]. (nig.ac.jp)
  • Causative agents were detected by blood cultures in 48 cases. (cdc.gov)
  • All 62 blood culture-negative endocarditis cases were tested by serologic or molecular methods or both. (cdc.gov)
  • Most cases of blood culture-negative endocarditis have been thought to be caused by previous antimicrobial therapy. (cdc.gov)
  • But within few years of successive culture, farmers faced a bitter experience, most of the culture ponds became hypernutrified which in extreme cases caused eutrophication by algal population due to continuous deposition of nutrients from unutilized natural food, excessive supplementary feed, uneaten portion of feed and excretory products. (medcraveonline.com)
  • 1:800) and 7 minor criteria (positive blood cultures, fever, previous heart disease, arterial embolism, positive results on serologic examination for endocarditis bacterial pathogens, immunologic disorders, and atypical but compatible findings on echocardiography) ( 12 ). (cdc.gov)