A specific protein in egg albumin that interacts with BIOTIN to render it unavailable to mammals, thereby producing biotin deficiency.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
A 60-kDa extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.
Incorporation of biotinyl groups into molecules.

Adhesion energy of receptor-mediated interaction measured by elastic deformation. (1/556)

We investigated the role of receptor binding affinity in surface adhesion. A sensitive technique was developed to measure the surface energy of receptor-mediated adhesion. The experimental system involved a functionalized elastic agarose bead resting on a functionalized glass coverslip. Attractive intersurface forces pulled the two surfaces together, deforming the bead to produce an enlarged contact area. The Johnson-Kendall-Roberts (JKR) model was used to relate the surface energy of the interaction to the elasticity of the bead and the area of contact. The surface energies for different combinations of modified surfaces in solution were obtained from reflection interference contrast microscopy (RICM) measurements of the contact area formed by the bead and the coverslip. Studies with surfaces functionalized with ligand-receptor pairs showed that the relationship between surface energy and the association constant of the ligand binding has two regimes. At low binding affinity, surface energy increased linearly with the association constant, while surface energy increased logarithmically with the association constant in the high affinity regime.  (+info)

Advanced analysis of biotin metabolites in body fluids allows a more accurate measurement of biotin bioavailability and metabolism in humans. (2/556)

In previous studies, the bioavailability of biotin in humans was estimated from the recovery of biotin in urine; urinary biotin was measured by microbial growth assays or assays of avidin-binding activity. These assays underestimate concentrations of biotin metabolites, which originate from beta-oxidation, sulfur oxidation or a combination. We have developed an HPLC/avidin-binding assay that is specific for biotin and its metabolites. With the use of the HPLC/avidin-binding assay, TLC and derivatization with p-dimethylaminocinnamaldehyde, we have identified and quantitated biotin and metabolites in urine from six healthy adults. Of that total, biotin accounted for 32+/-12%, bisnorbiotin for 52+/-15%, bisnorbiotin methyl ketone for 7.9+/-5.8%, biotin-d,l-sulfoxide for 4.0+/-3.2% and biotin sulfone for 3.6+/-1.9%. After intravenous administration of 18.4 micromol of biotin, the urinary excretion of biotin metabolites increased 21-130 times above baseline values. Because the biliary excretion of biotin is quantitatively minor (1.9+/-0.2% of an intravenous [14C]biotin dose in rats), intravenously administered biotin is not exposed to intestinal microorganisms. Thus we conclude that biotin metabolites in human urine originate from biotin catabolism in human tissues rather than biotin catabolism by intestinal microorganisms. With the use of the HPLC/avidin-binding assay, we estimated the bioavailability of biotin in adults from the urinary excretion of biotin and metabolites after ingestion of 2.1, 8.2 and 81.9 micromol of biotin. These data provide evidence that biotin is nearly completely absorbed.  (+info)

Accurate measurement of avidin and streptavidin in crude biofluids with a new, optimized biotin-fluorescein conjugate. (3/556)

A new biotin-fluorescein conjugate with an ethylene diamine spacer was found to be the first fluorescent biotin derivative which truly mimicked d-biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. These exceptional properties were attributed to the small size/length of the new ligand since all larger/longer biotin derivatives are known for their mutual steric hindrance and anti-cooperative binding in 4:1 complexes with avidin and streptavidin tetramers. Specific binding of the new biotin-fluorescein conjugate towards avidin and streptavidin was accompanied by 84-88% quenching of ligand fluorescence. In the accompanying study this effect was used for rapid estimation of avidin and streptavidin in a new 'single tube assay'. In the present study the strong quenching effect was utilized to accurately monitor stoichiometric titration of biotin-binding sites in samples with >/=200 pM avidin or streptavidin. The concentration was calculated from the consumption of fluorescent ligand up to the distinct breakpoint in the fluorescence titration profile which was marked by the abrupt appearance of strongly fluorescent ligands which were in excess. Due to this protocol the assay was not perturbed by background fluorescence or coloration in the unknown samples. The new fluorescence titration assay is particularly suited for quick checks on short notice because getting started only means to thaw an aliquot of a standardized stock solution of fluorescent ligand. No calibration is required for the individual assay and the ligand stock solution needs to be restandardized once per week (or once per year) when stored at -25 degrees C (or at -70 degrees C, respectively).  (+info)

Rapid estimation of avidin and streptavidin by fluorescence quenching or fluorescence polarization. (4/556)

A new biotin-carboxyfluorescein conjugate has been presented in the accompanying study (G. Kada et al., Biochim. Biophys. Acta 000 (1999) 000-000) which contains ethylene diamine as a 4-atom spacer. This so-called biotin-4-fluorescein showed exceptionally fast and tight binding to avidin and streptavidin, and binding was accompanied by strong quenching. In the present study the specific quenching of 'biotin-4-fluorescein' was utilized to measure (strept)avidin concentrations (0.2-2 nM) by the extent of fluorescence quenching at 8 nM ligand concentration. Adsorption of (strept)avidin to the assay tubes was suppressed by inclusion of bovine serum albumin (0.1 mg/ml). Virtually the same specific response to avidin and streptavidin was also observed with commercial 'fluorescein-biotin', except that >10 h incubation times were required. The slow association of 'fluorescein-biotin' was attributed to the anti-cooperative binding which is due to the much longer spacer as compared to 'biotin-4-fluorescein'. The third ligand tested in this study was 'biotin-4-FITC' which was analogous to 'biotin-4-fluorescein' except that carboxyfluorescein was replaced by the fluorescein isothiocyanate residue. Surprisingly, this probe was much less quenched by avidin but this was compensated by an exceptionally high fluorescence polarization in the avidin-bound state. In conclusion, the new ligand 'biotin-4-fluorescein' appeared to be the most general and convenient probe: quenching was most pronounced and linearly dependent on (strept)avidin concentrations, the dose response for streptavidin was almost the same as for avidin, and the association kinetics were fast enough to reach equilibrium within 30 min incubation time.  (+info)

The relationship of glycosylation and isoelectric point with tumor accumulation of avidin. (5/556)

Radiolabeled avidin markedly accumulated in intraperitoneal tumors and was cleared rapidly from circulation when given intraperitoneally. This study investigated the mechanisms of the tumor localization of avidin. METHODS: Avidin was deglycosylated through endoglycosydase-H digestion and/or neutralized by acetylation of its lysine amino acids with acetic acid N-hydroxysuccinimide ester. Avidin and modified avidins were analyzed using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS/PAGE) and isoelectric focusing. A tumor model was established by intraperitoneal injection of human colon cancer cells, LS180, in nude mice. Avidin and modified avidins were labeled with 111In using diethyleneamine pentaacetic acid-biotin and were administered intraperitoneally into the tumor-bearing mice. The biodistribution of radioactivity was examined 2 and 24 h postinjection. RESULTS: Deglycosylated avidins revealed a major band of smaller molecules on SDS/PAGE. The isoelectric point of neutralized avidins was reduced to less than 5, whereas that of unneutralized avidins was more than 9.5. Biodistribution study demonstrated that liver uptake was decreased by deglycosylation and kidney accumulation was decreased by neutralization, respectively. The blood clearance was remarkably slowed by combined modification of deglycosylation and neutralization. The tumor uptake of radioactivity was reduced by either deglycosylation or neutralization and was further decreased with combined modification. CONCLUSION: Both high glycosylation and positive charge of avidin contributed to its accumulation in tumor. This study may facilitate development of a new vehicle for the delivery of therapeutic agents to intraperitoneal tumors.  (+info)

Orientation of the pore-forming peptide GALA in POPC vesicles determined by a BODIPY-avidin/biotin binding assay. (6/556)

We determined the orientation of a biotinylated version of the pore-forming peptide GALA (WEAALAEALAEALAEHLAEALAEALEALAA) at pH 5.0 in large unilamellar phosphatidylcholine vesicles, using the enhancement of BODIPY-avidin fluorescence subsequent to its irreversible binding to a biotin moiety. GALA and its variants were biotinylated at the N- or C-terminus. BODIPY-avidin was either added externally or was pre-encapsulated in vesicles to assess the fraction of liposome-bound biotinylated GALA that exposed its labeled terminus to the external or internal side of the bilayer, respectively. Under conditions where most of the membrane-bound peptides were involved in transmembrane aggregates and formed aqueous pores (at a lipid/bound peptide molar ratio of 2500/1), the head-to-tail (N- to C-terminus) orientation of the membrane-inserted peptides was such that 3/4 of the peptides exposed their N-terminus on the inside of the vesicle and their C-terminus on the outside. Under conditions resulting in reduced pore formation (at higher lipid/peptide molar ratios), we observed an increase in the fraction of GALA termini exposed to the outside of the vesicle. These results are consistent with a model (Parente et al., Biochemistry, 29:8720, 1990) that requires a critical number of peptides (M) in an aggregate to form a transbilayer structure. When the peptides form an aggregate of size i, with i < M = 4 to 6, the orientation of the peptides is mostly parallel to the membrane surface, such that both termini of the biotinylated peptide are exposed to external BODIPY-avidin. This BODIPY-avidin/biotin binding assay should be useful to determine the orientation of other membrane-interacting molecules.  (+info)

Chemical cleavage of the overexpressed mitochondrial F1beta precursor with CNBr: a new strategy to construct an import-competent preprotein. (7/556)

We have isolated a soluble import-competent 15 kDa N-terminal fragment of the overexpressed Nicotiana plumbaginifolia F1beta precursor of the ATP synthase (N15pF1beta). The isolation was achieved after chemical cleavage, with CNBr, of the insoluble precursor collected in inclusion bodies, followed by purification of the fragment using ion-exchange chromatography. The purity of the final product was estimated to be more than 99%. N15pF1beta contained a presequence of 54 amino acid residues (except for the N-terminal methionine residue) and 82 N-terminal residues of the mature protein. N15pF1beta was shown to be imported into isolated potato tuber mitochondria and to be processed by the isolated mitochondrial processing peptidase (MPP) integrated into the cytochrome bc1 complex of the respiratory chain. Addition of N15pF1beta at micromolar concentrations resulted in the inhibition of import of F1beta precursor and alternative oxidase precursor, synthesized in vitro, into isolated mitochondria as well as the processing of these precursors catalysed by the isolated MPP-bc1 complex. N15pF1beta conjugated via a biotin link to avidin blocked import sites even after the reisolation of mitochondria and inhibited the import of the mitochondrial precursors, indicating that it can be used as a substrate for the generation of a stable translocation intermediate. Our results present a novel procedure for the production of an N-terminal fragment of the F1beta precursor that contains all information necessary for mitochondrial targeting and processing and that can be used for structural and functional studies of the mitochondrial protein import system. This procedure has a general value because it can be used for the production of chemical quantities of any mitochondrial import substrate and presequence peptide.  (+info)

Tumor pretargeting with avidin improves the therapeutic index of biotinylated tumor necrosis factor alpha in mouse models. (8/556)

The clinical use of tumor necrosis factor alpha (TNF) as an anticancer drug is limited to local or locoregional administration because of dose-limiting systemic toxicity. We investigated in animal models whether the therapeutic index of systemically administered human or murine TNF can be increased by tumor pretargeting strategies based on the biotin-avidin system. Pretargeting of s.c. mouse WEHI-164 fibrosarcoma and RMA lymphoma genetically engineered to express the Thy 1.1 antigen on the cell membrane was achieved by i.p. injection of a biotinylated anti-Thy 1.1 antibody and avidin. This pretreatment increased the antitumor activity of systemically administered biotin-TNF conjugates by at least 5-fold. In contrast, pretargeting did not increase the toxicity of biotin-TNF, as judged by animal survival and weight loss after treatment. Ex vivo analysis of tumor cells 24 h after treatment showed that biotin-TNF persisted for several hours on the surface of pretargeted tumors, but not when avidin was omitted. The potentiation of the antitumor effects was related primarily to indirect mechanisms, involving a host-mediated response. The results indicate that tumor pretargeting improves the antitumor activity of TNF. Tumor pretargeting with avidin, which is currently used to increase the uptake of radioactive-labeled biotin in patients, could represent a new strategy for improving the therapeutic index of TNF.  (+info)

Avidin is a protein found in the white of eggs (egg whites) and some other animal tissues. It has a high binding affinity for biotin, also known as vitamin B7 or vitamin H, which is an essential nutrient for humans and other organisms. This property makes avidin useful in various biochemical and medical applications, such as immunohistochemistry, blotting techniques, and drug delivery systems.

Biotin-avidin interactions are among the strongest non-covalent interactions known in nature, with a dissociation constant (Kd) of approximately 10^-15 M. This means that once biotin is bound to avidin, it is very difficult to separate them. In some cases, this property can be exploited to create stable and specific complexes for various applications.

However, it's worth noting that the high affinity of avidin for biotin can also have negative effects in certain contexts. For example, raw egg whites contain large amounts of avidin, which can bind to biotin in the gut and prevent its absorption if consumed in sufficient quantities. This can lead to biotin deficiency, which can cause various health problems. Cooking egg whites denatures avidin and reduces its ability to bind to biotin, making cooked eggs a safe source of biotin.

Biotin is a water-soluble vitamin, also known as Vitamin B7 or Vitamin H. It is a cofactor for several enzymes involved in metabolism, particularly in the synthesis and breakdown of fatty acids, amino acids, and carbohydrates. Biotin plays a crucial role in maintaining healthy skin, hair, nails, nerves, and liver function. It is found in various foods such as nuts, seeds, whole grains, milk, and vegetables. Biotin deficiency is rare but can occur in people with malnutrition, alcoholism, pregnancy, or certain genetic disorders.

Streptavidin is not a medical term per se, but rather a biochemical term used in the field of medicine and laboratory research. Streptavidin is a protein that is derived from the bacterium Streptomyces avidinii. It has a unique ability to bind very strongly and specifically to another molecule called biotin, with an association constant that is one of the strongest non-covalent interactions known in nature.

This property makes streptavidin a valuable tool in various medical and research applications such as immunoassays, histology, molecular biology, and drug delivery systems. For example, biotinylated molecules (such as antibodies, DNA, or enzymes) can be linked to streptavidin for detection, purification, or targeting purposes.

In summary, streptavidin is a bacterial protein that binds strongly and specifically to biotin, which is used in various medical and research applications as a tool for detection, purification, or targeting purposes.

Biotinyllation is a process of introducing biotin (a vitamin) into a molecule, such as a protein or nucleic acid (DNA or RNA), through chemical reaction. This modification allows the labeled molecule to be easily detected and isolated using streptavidin-biotin interaction, which has one of the strongest non-covalent bonds in nature. Biotinylated molecules are widely used in various research applications such as protein-protein interaction studies, immunohistochemistry, and blotting techniques.

The monomeric avidin is created by treatment of immobilized native avidin with urea or guanidine HCl (6-8 M), giving it a lower ... Dimeric members of the avidin family are also found in some bacteria. In chicken egg white, avidin makes up approximately 0.05 ... A 1991 assay for the Journal of Food Science detected substantial avidin activity in cooked egg white: "mean residual avidin ... Aware of the strength and specificity of the avidin-biotin complex, researchers began to exploit chicken avidin and ...
Green NM (1975). "Avidin". Advances in Protein Chemistry. 29: 85-133. doi:10.1016/S0065-3233(08)60411-8. ISBN 9780120342297. ...
Green NM (1975). "Avidin". Advances in Protein Chemistry. 29: 85-133. doi:10.1016/s0065-3233(08)60411-8. ISBN 9780120342297. ...
Avidin is the other most notable biotin-binding protein. Originally isolated from egg yolk, avidin only has 30% sequence ... Avidin has a higher affinity for biotin (Kd ~ 10−15M) but in contrast to streptavidin, avidin is glycosylated, positively ... it has the advantage of much lower nonspecific binding than avidin. Deglycosylated avidin (NeutrAvidin) is more comparable to ... On the other hand, streptavidin is the better biotin-conjugate binder; avidin has a lower binding affinity than streptavidin ...
"Meeduma Wage Avidin muhurath ceremony". Sarasaviya. Retrieved 24 August 2019. "The Journey of Sri Lanka's iconic entertainer ... Kandayama Ira Awara Isuru Pawura Kalu Kumari Kasee Salu Kinduru Adaviya Kolam Kuttama Mahathala Hatana Meeduma Wage Avidin ...
"Sudu Andagena Kalu Avidin in Rupavahini". Sarasaviya (in Sinhala). 21 March 2019. Retrieved 21 March 2019. "Mesmerising ... "Sudu Andagena Kalu Avidin in sri lanka Rupavahini Corporation" (in Sinhala). Mawbima. 23 March 2019. Retrieved 23 March 2019. " ...
"Sudu Andagena Kalu Avidin in sri lanka Rupavahini Corporation" (in Sinhala). Mawbima. 23 March 2019. Retrieved 23 March 2019. " ... "Sudu Andagena Kalu Avidin in Rupavahini". Sarasaviya (in Sinhala). 21 March 2019. Retrieved 21 March 2019. "Michelle has been ...
"Sudu Andagena Kalu Avidin in Rupavahini". Sarasaviya (in Sinhala). 21 March 2019. Retrieved 21 March 2019. "Michelle talks ...
"Sudu Andagena Kaḷu Avidin wins awards". Sarasaviya. Retrieved 23 June 2022. "All about Chandali". Sarasaviya. Retrieved 23 June ...
Livnah, O., Bayer, E.A., Wilchek, M., and Sussman, J. (1993). "Three-dimensional structures of avidin and the avidin-biotin ... and the avidin-biotin system. The avidin-biotin complex is the highest affinity interaction in nature, and its utilization to ... Consequently, the avidin-biotin system can frequently replace radioactive probes. Together with Ed Bayer, Wilchek established ... Early in the 1970s, they exploited Avidin as a probe and developed new methods and reagents to biotinylate antibodies and other ...
Biotinylated plasmid DNA was bound by avidin. New protein was synthesized by using cell-free expression system i.e. rabbit ...
"Avidin-Biotin Interaction - CA". www.thermofisher.com. Retrieved 2019-03-01. Kozubek S, Lukásová E, Amrichová J, Kozubek M, ...
"Components of Avidin-Biotin Technology: A Handbook." Pierce Biotechnology, Inc. Rockford, IL: June, 2003. "Photobiotin acetate ... Dontha, Narasaiah; Nowall, Wilbur B.; Kuhr, Werner G. (1997). "Generation of Biotin/Avidin/Enzyme Nanostructures with Maskless ...
Michael Green, N. (1990), "[5] Avidin and streptavidin", Avidin-Biotin Technology, Methods in Enzymology, vol. 184, Elsevier, ... After the discovery of the fast interaction between Biotin and (Strept)Avidin, which show a high binding affinity, this ... Hnatowich, D. J.; Virzi, F.; Rusckowski, M. (August 1987). "Investigations of avidin and biotin for imaging applications". ...
"Immobilized nitro-avidin and nitro-streptavidin as reusable affinity matrices for application in avidin-biotin technology". ... Because egg-derived avidin binds strongly to biotin with a dissociation constant Kd ≈ 10−15 M, biotinylated compounds of ... Second, the sample is incubated with avidin bound to beads, then rinsed, removing all unbound proteins, while leaving only the ... Gyorgy P, Rose CS (1943). "The Liberation of Biotin from the Avidin-Biotin Complex (AB)". Experimental Biology and Medicine. 53 ...
"Organelle pH studies using targeted avidin and fluorescein-biotin". Chemistry & Biology. 7 (3): 197-209. doi:10.1016/S1074-5521 ...
"Artificial metalloenzymes for enantioselective catalysis based on biotin-avidin". Journal of the American Chemical Society. 125 ...
A new avidin-biotin ELISA assay for its measurement". Journal of Immunoassay. 13 (1): 47-60. doi:10.1080/15321819208019824. ...
The biotin-avidin system quickly became a central method in cell biology, immunology, and protein engineering, as well as ... Heitzmann H, Richards FM (1974). "Use of the avidin-biotin complex for specific staining of biological membranes in electron ... Diamandis EP, Christopoulos TK (1991). "The biotin-(strept)avidin system: principles and applications in biotechnology". ... new types of chemical tags such as biotin/avidin, the nuclear magnetic resonance (NMR) chemical shift index, and structural and ...
Gyorgy, P.; Rose, C. S. (1943). "The Liberation of Biotin from the Avidin-Biotin Complex (AB)". Experimental Biology and ... work on biotin and in 1941 published a paper demonstrating that egg-white injury was caused by the binding of biotin by avidin ...
"Adoptive immunotherapy by avidin-driven cytotoxic T lymphocyte-tumor bridging". Cancer Research. 60 (15): 4211-5. PMID 10945632 ...
Livnah, O.; Bayer, E.; Wilchek, M.; Sussman, J. (1993). "Three-dimensional structures of avidin and the avidin-biotin complex ... Biotin and avidin bind with a dissociation constant of roughly 10−15 M = 1 fM = 0.000001 nM.Ribonuclease inhibitor proteins may ...
"Sudu Andagena Kalu Avidin in sri lanka Rupavahini Corporation" (in Sinhala). Mawbima. 23 March 2019. Retrieved 23 March 2019. " ...
"Sudu Andagena Kalu Avidin in sri lanka Rupavahini Corporation" (in Sinhala). Mawbima. 23 March 2019. Retrieved 23 March 2019. " ...
"Sudu Andagena Kalu Avidin in sri lanka Rupavahini Corporation" (in Sinhala). Mawbima. 23 March 2019. Retrieved 23 March 2019. " ...
She acted in a negative role in the drama Oba Ayemath Avidin. Anuradha was represented as a judge in many reality shows such as ... Maha Polowa Ape Newei Oba Ayemath Avidin Pinibara Yamaya Sakisanda Suwaris Wahinna Muthu Wessak Her acting career made a ...
The avidin-peroxidase labeling TUNEL assay is applicable for light absorption microscopy. Many TUNEL-related kits are ...
Avidin is an antinutrient found in active form in raw egg whites. It binds very tightly to biotin (vitamin B7) and can cause ...
Previously, a biotin-avidin magnetic bead system was used for this purpose. BioTechniques. 1998 Sep;25(3):516-21. Mutation ...
When cooked, avidin is partially denatured and binding to biotin is reduced. However one study showed that 30-40% of the avidin ... Egg whites contain high levels of avidin, a protein that binds biotin strongly. ...
The monomeric avidin is created by treatment of immobilized native avidin with urea or guanidine HCl (6-8 M), giving it a lower ... Dimeric members of the avidin family are also found in some bacteria. In chicken egg white, avidin makes up approximately 0.05 ... A 1991 assay for the Journal of Food Science detected substantial avidin activity in cooked egg white: "mean residual avidin ... Aware of the strength and specificity of the avidin-biotin complex, researchers began to exploit chicken avidin and ...
More info for Family b.61.1.1: Avidin/streptavidin. Timeline for Family b.61.1.1: Avidin/streptavidin: *Family b.61.1.1: Avidin ... Lineage for Family b.61.1.1: Avidin/streptavidin. *Root: SCOP 1.55 *. Class b: All beta proteins [48724] (93 folds). ... Family b.61.1.1: Avidin/streptavidin appears in the current release, SCOPe 2.08. ...
Investigations of Avidin and Biotin for Imaging Applications Message Subject (Your Name) has sent you a message from Journal of ... Investigations of Avidin and Biotin for Imaging Applications. D.J. Hnatowich, F. Virzi and M. Rusckowski ... Biochemical and Biological Characterization of a New Oxidized Avidin with Enhanced Tissue Binding Properties ...
Development of an avidin-biotin dot enzyme-linked immunosorbent assay and its comparison with other serological tests for ... View more info for Development of an avidin-biotin dot enzyme-linked immunosorbent assay and its comparison with other ... the reaction being amplified by an avidinbiotin system with biotinylated anti-horse IgG and horseradish peroxidase-avidin D. ...
Laboratory mice are commonly used in animal models for cognition studies - several of which are offered by us. These tests consist of administering drugs to mice and then testing various aspects of their behavior that reflect cognitive functions (like memory, spatial learning, social interactions, anxiety and depression). Y-Maze Test…
... Reactive Dyes & Labeling Kits (32) Cellular & Molecular Detection (13) Crosslinkers & ... Reactive Tags (7) Nanoparticles & Beads (1) Bioconjugates & Services (20) - Avidin and streptavidin conjugates (1) - ...
1:200 avidin-FITC (Vector Antibodies). 1:200 anti-avidin biotinylated. *All antibodies are diluted in 4X SSC/Tween 20/BSA.. ... Make enough avidin mix for at least one more sample than the actual number of samples (recipe follows). Add 100 µL of avidin ... Resuspend pellet in 100 µL of buffer made of 4X SSC with 0.1% Triton X-100, 1% BSA and 2.5 µg/mL avidin FITC. Incubate for 30 ...
from egg white. 10-14 units/mg of solid. product code: AVN 1 unit equals the protein required to bind 1 microgram of d-biotin at pH8.9
... deglycosylated avidin purified from egg white Shop SouthernBiotech™ Neutralite Avidin at Fishersci.com ...
Return to Article Details Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation Download Download ...
View Mouse EphB2 Biotinylated Antibody (BAF467) datasheet.
查看 Human TrkB Biotinylated Antibody (BAF397) 数据表.
Avidin & Biotinylated Reagents (24). Specialty Products (7). Antibodies (97). VECTASTAIN ABC (Avidin-Biotin Complex Kits) (26) ...
Avidin. 1. The use of (14-C)biotin for kinetic studies and for assay. Biochem. J. 1963;89:585-591. View abstract. ... Concentrationand assay of avidin, injury-producing agents in raw egg white. J Biol Chem. 1941;:535-43. ...
Avidin-dependent redirection of transduction through a variety of biotinylated ligands is greatly dependent on the nature of ... A novel system for the attachment of targeting ligands to the Ad capsid, based on the extremely strong avidin-biotin ... Avidin-dependent redirection of transduction through a variety of biotinylated ligands is greatly dependent on the nature of ... A novel system for the attachment of targeting ligands to the Ad capsid, based on the extremely strong avidin-biotin ...
"Sudu Andagena Kalu Avidin in Rupavahini". Sarasaviya (in Sinhala). 21 March 2019. Retrieved 21 March 2019. "Mesmerising ... "Sudu Andagena Kalu Avidin in sri lanka Rupavahini Corporation" (in Sinhala). Mawbima. 23 March 2019. Retrieved 23 March 2019. " ...
... blocking endogenous avidin and biotin (Cat. No. 00-4303, Invitrogen, Carlsbad, CA) and staining overnight with preassembled ...
Targeted labeling of cancer cells using biotin tagged avidin functionalized biocompatible Targeted labeling of cancer cells ... The present study details the development of biotin tagged avidin functionalized Zinc Sulphide [ZnS] nanocrystals through a ... Further biotinylation of these particles through the strong non-covalent interaction between biotin and avidin enabled highly ... using biotin tagged avidin functionalized biocompatible fluorescent nanocrystals. Aswathy, J; Jahnavi, S; Krishna, R; Manzoor, ...
Avidin. 60 °C - 103 bp. Forward 5′ - 3′. TCGTTTCTCCTCTGACGGGCT. Reverse 5′ - 3′. TGCCCTGCCCAGGTGGTGAT. ...
The biotin-avidin bond is essentially irreversible; as a result, biotin is not liberated from food, and the biotin-avidin ... Heat destroys the avidin, so those who eat cooked eggs are not at risk of biotin deficiency. Raw egg whites lead to biotin ... Avidin, a protein found in egg whites, binds strongly to biotin, impairing the absorption of the vitamin, leading to severe ... The ureido ring is involved in the high affinity binding of biotin to avidin, a glycoprotein found in egg-white. A valeric acid ...
... laser scanning microscope by incubation bacterial cells with biotinylated bovine lactoferrin and FITC-conjugated avidin. ...
... the avidin-biotin complex is the most popular immunhistochemistry method today. ... With over 100K citations and counting, the avidin-biotin complex is the most popular immunhistochemistry method today.. The ... Sensitive, reliable, and reproducible, the avidin-biotin complex is the most widely used immunohistochemistry method. Vector ...
Signals were detected with fluorescein avidin DN (Vector Laboratories, Burlingame, CA, USA). Chromosomes were counterstained ...
Endogenous Biotin or Avidin/Streptavidin Binding Proteins. *. Endogenous Human Ig. *. Endogenous Mouse Ig ...
Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains. MeSH, 1998. Surface ... avidin: Protein found in raw egg white which binds to biotin (etymology is from its avidity to biotin). The interaction of ... Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the Avidin- ... Biotin-binding to streptavidin and avidin is resistant to extremes of heat, pH and proteolysis, making capture of biotinylated ...
Bubb, M.O.; Green, F.; Conradie, J.D.; Tchernyshev, B.; Bayer, E.A.; Wilchek, M. Natural antibodies to avidin in human serum. ... Ninety-six-well MaxiSorp microtiter plates (Corning, Corning, NY, USA) were precoated with 1 μg/mL avidin in PBS at 4 °C ... Thus, we developed an ELISA to screen anti-AQP5 autoantibodies by utilizing an avidin-biotinylated peptide coating system that ... To remove natural antibodies to avidin in human serum [17] and nonspecifically bound antibodies, the concentration of ...
Conjugation with avidin-biotin complex (Vecstatin Elite ABC kit; Vector Laboratories) was followed by visualization with 3,3′- ... Conjugation with avidin-biotin complex (Vecstatin Elite ABC kit; Vector Laboratories) was followed by visualization with DAB ( ...
  • Aware of the strength and specificity of the avidin-biotin complex, researchers began to exploit chicken avidin and streptavidin as probes and affinity matrices in numerous research projects. (wikipedia.org)
  • The interaction of biotin and Avidin or Streptavidin has been exploited for use in many protein and nucleic acid detection and purification methods. (genomicglossaries.com)
  • Biotin-binding to streptavidin and avidin is resistant to extremes of heat, pH and proteolysis, making capture of biotinylated molecules possible in a wide variety of environments. (genomicglossaries.com)
  • The overall staining procedure uses one less reagent (streptavidin peroxidase) and rinsing step, eliminates avidin-biotin blocking procedures and may reduce overall staining time. (abcam.com)
  • and the solubility of streptavidin (isoelectric pH5) in aqueous buffer is much lower than avidin.The binding of streptavidin is similar to that of avidin with less non-specific binding due to the lack of carbohydrate groups. (thomassci.com)
  • the binding of streptavidin to biotin is similar to that of avidin. (thomassci.com)
  • The test was based on the detection of IgG antibodies to Pseudomonas mallei antigens bound to nitrocellulose coated on plastic strips (dipsticks), the reaction being amplified by an avidinbiotin system with biotinylated anti-horse IgG and horseradish peroxidase-avidin D. Sera from 810 normal, six naturally infected and 48 sensitized equines were tested by this assay and results were compared with complement fixation, indirect haemagglutination and counter-immunoelectrophoresis tests. (edu.in)
  • Visualization of the human immunoglobulins specifically bound to HIV-1 proteins is accomplished in situ by using a series of reactions with goat anti-human IgG conjugated with biotin, avidin conjugated with horseradish peroxidase (HRP), and the HRP substrate 4-chloro-1-naphthol. (cdc.gov)
  • as a result, biotin is not liberated from food, and the biotin-avidin complex is lost in the feces. (medscape.com)
  • the biotin-avidin complex is not broken during passage of the food bolus through the stomach and intestines. (medscape.com)
  • Soon after, researchers Bayer and Wilchek developed new methods and reagents to biotinylate antibodies and other biomolecules, allowing the transfer of the avidin-biotin system to a range of biotechnological applications. (wikipedia.org)
  • For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support. (wikipedia.org)
  • Deglycosylated, neutral forms of chicken avidin are available through Sigma-Aldrich (Extravidin), Thermo Scientific (NeutrAvidin), Invitrogen (NeutrAvidin), and e-Proteins (NeutraLite). (wikipedia.org)
  • Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. (bdbiosciences.com)
  • The final step in solving the mystery of egg-white injury syndrome was the demonstration that the syndrome could be prevented by heating the egg whites, a process that denatures avidin and destroys its affinity for biotin. (medscape.com)
  • Cooking denatures avidin, a term used when the shape of a protein is changed. (huffpost.com)
  • Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. (abcam.com)
  • The ureido ring is involved in the high affinity binding of biotin to avidin, a glycoprotein found in egg-white. (medscape.com)
  • The dissociation constant of the avidin-biotin complex is measured to be KD ≈ 10−15 M, making it one of the strongest known non-covalent bonds. (wikipedia.org)
  • Given the strength of the avidin-biotin bond, dissociation of the avidin-biotin complex requires extreme conditions that cause protein denaturation. (wikipedia.org)
  • The non-reversible nature of the avidin-biotin complex can limit avidin's application in affinity chromatography applications where release of the captured ligand is desirable. (wikipedia.org)
  • With over 100K citations and counting, the avidin-biotin complex is the most popular immunhistochemistry method today. (the-scientist.com)
  • Sensitive, reliable, and reproducible, the avidin-biotin complex is the most widely used immunohistochemistry method. (the-scientist.com)
  • Sequential serial paraffin sections from each specimen were prepared for immunohistochemical staining using an avidin-biotin-complex (ABC) technique [12]. (who.int)
  • Neutravidin, a deglycosylated avidin with modified arginines, exhibits a more neutral isoelectric point (pI) and is available as an alternative to native avidin, whenever problems of non-specific binding arise. (wikipedia.org)
  • The name of the protein was later revised to "avidin" based on its affinity for biotin (avid + biotin). (wikipedia.org)
  • The biochemical basis for egg-white injury syndrome was quickly elucidated when raw egg whites were found to contain the glycoprotein avidin, which has a remarkable affinity for biotin. (medscape.com)
  • citation needed] As a basically charged glycoprotein, avidin exhibits non-specific binding in some applications. (wikipedia.org)
  • Avidin, a protein found in egg whites, binds strongly to biotin, impairing the absorption of the vitamin, leading to severe biotin deficiency in those who consume excessive amounts of raw eggs. (medscape.com)
  • Raw eggs contain a protein called avidin, which binds and inactivates biotin. (huffpost.com)
  • Bound antibody was detected using avidin-biotin-peroxidase (Vector Labs) and diaminobenzidine (DAB). (cellimagelibrary.org)
  • Further biotinylation of these particles through the strong non-covalent interaction between biotin and avidin enabled highly specific labeling of the biotin receptors on human hepatocellular carcinoma (HepG2) cells . (bvsalud.org)
  • A novel system for the attachment of targeting ligands to the Ad capsid, based on the extremely strong avidin-biotin interaction, is described herein. (rice.edu)
  • Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the Avidin-biotin interaction to be used for the development of robust and highly sensitive assays. (genomicglossaries.com)
  • After treatment, rats were euthanized, and tail tissues were chemically fixed for histological analysis of nerves (PGP9.5, neuropeptide Y, calcitonin gene related protein) and mast cells (avidin, Alcian Blue/Safranin O).2 Single sections were doublestained for nerve fibers and mast cells to permit assessment of the degree of physical overlap. (cdc.gov)
  • The present study details the development of biotin tagged avidin functionalized Zinc Sulphide [ZnS] nanocrystals through a simple aqueous chemistry route at room temperature for targeted imaging applications. (bvsalud.org)
  • In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1800 μg per egg). (wikipedia.org)
  • Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. (wikipedia.org)
  • Functional avidin is found in raw egg, but depending on the amount of heat it is exposed to during cooking, the quantity of molecules available for binding biotin can change. (wikipedia.org)
  • Surface functionalization of Manganese doped ZnS nanocrystals with L-cysteine provided functional groups that facilitated its conjugation to avidin . (bvsalud.org)
  • The natural function of avidin in eggs is not known, although it has been postulated to be made in the oviduct as a bacterial growth inhibitor, by binding biotin helpful for bacterial growth. (wikipedia.org)
  • Denatured avidin does not bind biotin, so cooked eggs are a good source of bioavailable biotin. (huffpost.com)
  • Today, avidin is used in a variety of applications ranging from research and diagnostics to medical devices and pharmaceuticals. (wikipedia.org)
  • Dimeric members of the avidin family are also found in some bacteria. (wikipedia.org)
  • The modified avidin exhibits strong biotin binding characteristics at pH 4 and releases biotin at a pH of 10 or higher. (wikipedia.org)
  • In its tetrameric form, avidin is estimated to be 66-69 kDa in size. (wikipedia.org)
  • Targeted labeling of cancer cells using biotin tagged avidin functionalized biocompatible fluorescent nanocrystals. (bvsalud.org)
  • Cell binding ability of lactoferrin was inspected under an inverted confocal laser scanning microscope by incubation bacterial cells with biotinylated bovine lactoferrin and FITC-conjugated avidin. (nih.gov)
  • CD34+ cells were enriched from marrows of five baboons using avidin-biotin immunoadsorption. (nih.gov)
  • Discussion: PGP9.5, NPY and CGRP staining of nerve fibers and avidin-positive mast cells is feasible in rat tail sections. (cdc.gov)
  • Research in the 1970s helped establish the avidin-biotin system as a powerful tool in biological sciences. (wikipedia.org)
  • The presence of FGF-10 was detected by means of the avidin-biotin-peroxidase immunohistochemistry technique. (bvsalud.org)
  • Avidin-dependent redirection of transduction through a variety of biotinylated ligands is greatly dependent on the nature of the biotinylated capsid protein. (rice.edu)