A specific protein in egg albumin that interacts with BIOTIN to render it unavailable to mammals, thereby producing biotin deficiency.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
A 60-kDa extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.
Incorporation of biotinyl groups into molecules.
The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The white of an egg, especially a chicken's egg, used in cooking. It contains albumin. (Random House Unabridged Dictionary, 2d ed)
Cold-blooded, air-breathing VERTEBRATES belonging to the class Reptilia, usually covered with external scales or bony plates.
VERTEBRATES belonging to the class amphibia such as frogs, toads, newts and salamanders that live in a semiaquatic environment.
Warm-blooded VERTEBRATES possessing FEATHERS and belonging to the class Aves.
The use of community resources, individual case work, or group work to promote the adaptive capacities of individuals in relation to their social and economic environments. It includes social service agencies.
The science and technology dealing with the procurement, breeding, care, health, and selection of animals used in biomedical research and testing.
The protection of animals in laboratories or other specific environments by promoting their health through better nutrition, housing, and care.
An indirect sympathomimetic. Tyramine does not directly activate adrenergic receptors, but it can serve as a substrate for adrenergic uptake systems and monoamine oxidase so it prolongs the actions of adrenergic transmitters. It also provokes transmitter release from adrenergic terminals. Tyramine may be a neurotransmitter in some invertebrate nervous systems.
Radiation from sources other than the source of interest. It is due to cosmic rays and natural radioactivity in the environment.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
Organizations established by endowments with provision for future maintenance.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
The study of MAGNETIC PHENOMENA.
Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads.
A biocompatible polymer used as a surgical suture material.
Relating to the size of solids.
Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3.
A carboxy-lyase that catalyzes the decarboxylation of (S)-2-Methyl-3-oxopropanoyl-CoA to propanoyl-CoA. In microorganisms the reaction can be coupled to the vectorial transport of SODIUM ions across the cytoplasmic membrane.
A biotin-dependent enzyme belonging to the ligase family that catalyzes the addition of CARBON DIOXIDE to pyruvate. It is occurs in both plants and animals. Deficiency of this enzyme causes severe psychomotor retardation and ACIDOSIS, LACTIC in infants. EC 6.4.1.1.
Precise and detailed plans for the study of a medical or biomedical problem and/or plans for a regimen of therapy.
Compounds with three aromatic rings in linear arrangement with an OXYGEN in the center ring.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium such as the aqueous humor.
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Antibodies produced by a single clone of cells.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
A specialty field of radiology concerned with diagnostic, therapeutic, and investigative use of radioactive compounds in a pharmaceutical form.
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
Hospital department responsible for the administration and management of nuclear medicine services.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A publication issued at stated, more or less regular, intervals.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
The profession of writing. Also the identity of the writer as the creator of a literary production.
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.

Adhesion energy of receptor-mediated interaction measured by elastic deformation. (1/556)

We investigated the role of receptor binding affinity in surface adhesion. A sensitive technique was developed to measure the surface energy of receptor-mediated adhesion. The experimental system involved a functionalized elastic agarose bead resting on a functionalized glass coverslip. Attractive intersurface forces pulled the two surfaces together, deforming the bead to produce an enlarged contact area. The Johnson-Kendall-Roberts (JKR) model was used to relate the surface energy of the interaction to the elasticity of the bead and the area of contact. The surface energies for different combinations of modified surfaces in solution were obtained from reflection interference contrast microscopy (RICM) measurements of the contact area formed by the bead and the coverslip. Studies with surfaces functionalized with ligand-receptor pairs showed that the relationship between surface energy and the association constant of the ligand binding has two regimes. At low binding affinity, surface energy increased linearly with the association constant, while surface energy increased logarithmically with the association constant in the high affinity regime.  (+info)

Advanced analysis of biotin metabolites in body fluids allows a more accurate measurement of biotin bioavailability and metabolism in humans. (2/556)

In previous studies, the bioavailability of biotin in humans was estimated from the recovery of biotin in urine; urinary biotin was measured by microbial growth assays or assays of avidin-binding activity. These assays underestimate concentrations of biotin metabolites, which originate from beta-oxidation, sulfur oxidation or a combination. We have developed an HPLC/avidin-binding assay that is specific for biotin and its metabolites. With the use of the HPLC/avidin-binding assay, TLC and derivatization with p-dimethylaminocinnamaldehyde, we have identified and quantitated biotin and metabolites in urine from six healthy adults. Of that total, biotin accounted for 32+/-12%, bisnorbiotin for 52+/-15%, bisnorbiotin methyl ketone for 7.9+/-5.8%, biotin-d,l-sulfoxide for 4.0+/-3.2% and biotin sulfone for 3.6+/-1.9%. After intravenous administration of 18.4 micromol of biotin, the urinary excretion of biotin metabolites increased 21-130 times above baseline values. Because the biliary excretion of biotin is quantitatively minor (1.9+/-0.2% of an intravenous [14C]biotin dose in rats), intravenously administered biotin is not exposed to intestinal microorganisms. Thus we conclude that biotin metabolites in human urine originate from biotin catabolism in human tissues rather than biotin catabolism by intestinal microorganisms. With the use of the HPLC/avidin-binding assay, we estimated the bioavailability of biotin in adults from the urinary excretion of biotin and metabolites after ingestion of 2.1, 8.2 and 81.9 micromol of biotin. These data provide evidence that biotin is nearly completely absorbed.  (+info)

Accurate measurement of avidin and streptavidin in crude biofluids with a new, optimized biotin-fluorescein conjugate. (3/556)

A new biotin-fluorescein conjugate with an ethylene diamine spacer was found to be the first fluorescent biotin derivative which truly mimicked d-biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. These exceptional properties were attributed to the small size/length of the new ligand since all larger/longer biotin derivatives are known for their mutual steric hindrance and anti-cooperative binding in 4:1 complexes with avidin and streptavidin tetramers. Specific binding of the new biotin-fluorescein conjugate towards avidin and streptavidin was accompanied by 84-88% quenching of ligand fluorescence. In the accompanying study this effect was used for rapid estimation of avidin and streptavidin in a new 'single tube assay'. In the present study the strong quenching effect was utilized to accurately monitor stoichiometric titration of biotin-binding sites in samples with >/=200 pM avidin or streptavidin. The concentration was calculated from the consumption of fluorescent ligand up to the distinct breakpoint in the fluorescence titration profile which was marked by the abrupt appearance of strongly fluorescent ligands which were in excess. Due to this protocol the assay was not perturbed by background fluorescence or coloration in the unknown samples. The new fluorescence titration assay is particularly suited for quick checks on short notice because getting started only means to thaw an aliquot of a standardized stock solution of fluorescent ligand. No calibration is required for the individual assay and the ligand stock solution needs to be restandardized once per week (or once per year) when stored at -25 degrees C (or at -70 degrees C, respectively).  (+info)

Rapid estimation of avidin and streptavidin by fluorescence quenching or fluorescence polarization. (4/556)

A new biotin-carboxyfluorescein conjugate has been presented in the accompanying study (G. Kada et al., Biochim. Biophys. Acta 000 (1999) 000-000) which contains ethylene diamine as a 4-atom spacer. This so-called biotin-4-fluorescein showed exceptionally fast and tight binding to avidin and streptavidin, and binding was accompanied by strong quenching. In the present study the specific quenching of 'biotin-4-fluorescein' was utilized to measure (strept)avidin concentrations (0.2-2 nM) by the extent of fluorescence quenching at 8 nM ligand concentration. Adsorption of (strept)avidin to the assay tubes was suppressed by inclusion of bovine serum albumin (0.1 mg/ml). Virtually the same specific response to avidin and streptavidin was also observed with commercial 'fluorescein-biotin', except that >10 h incubation times were required. The slow association of 'fluorescein-biotin' was attributed to the anti-cooperative binding which is due to the much longer spacer as compared to 'biotin-4-fluorescein'. The third ligand tested in this study was 'biotin-4-FITC' which was analogous to 'biotin-4-fluorescein' except that carboxyfluorescein was replaced by the fluorescein isothiocyanate residue. Surprisingly, this probe was much less quenched by avidin but this was compensated by an exceptionally high fluorescence polarization in the avidin-bound state. In conclusion, the new ligand 'biotin-4-fluorescein' appeared to be the most general and convenient probe: quenching was most pronounced and linearly dependent on (strept)avidin concentrations, the dose response for streptavidin was almost the same as for avidin, and the association kinetics were fast enough to reach equilibrium within 30 min incubation time.  (+info)

The relationship of glycosylation and isoelectric point with tumor accumulation of avidin. (5/556)

Radiolabeled avidin markedly accumulated in intraperitoneal tumors and was cleared rapidly from circulation when given intraperitoneally. This study investigated the mechanisms of the tumor localization of avidin. METHODS: Avidin was deglycosylated through endoglycosydase-H digestion and/or neutralized by acetylation of its lysine amino acids with acetic acid N-hydroxysuccinimide ester. Avidin and modified avidins were analyzed using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS/PAGE) and isoelectric focusing. A tumor model was established by intraperitoneal injection of human colon cancer cells, LS180, in nude mice. Avidin and modified avidins were labeled with 111In using diethyleneamine pentaacetic acid-biotin and were administered intraperitoneally into the tumor-bearing mice. The biodistribution of radioactivity was examined 2 and 24 h postinjection. RESULTS: Deglycosylated avidins revealed a major band of smaller molecules on SDS/PAGE. The isoelectric point of neutralized avidins was reduced to less than 5, whereas that of unneutralized avidins was more than 9.5. Biodistribution study demonstrated that liver uptake was decreased by deglycosylation and kidney accumulation was decreased by neutralization, respectively. The blood clearance was remarkably slowed by combined modification of deglycosylation and neutralization. The tumor uptake of radioactivity was reduced by either deglycosylation or neutralization and was further decreased with combined modification. CONCLUSION: Both high glycosylation and positive charge of avidin contributed to its accumulation in tumor. This study may facilitate development of a new vehicle for the delivery of therapeutic agents to intraperitoneal tumors.  (+info)

Orientation of the pore-forming peptide GALA in POPC vesicles determined by a BODIPY-avidin/biotin binding assay. (6/556)

We determined the orientation of a biotinylated version of the pore-forming peptide GALA (WEAALAEALAEALAEHLAEALAEALEALAA) at pH 5.0 in large unilamellar phosphatidylcholine vesicles, using the enhancement of BODIPY-avidin fluorescence subsequent to its irreversible binding to a biotin moiety. GALA and its variants were biotinylated at the N- or C-terminus. BODIPY-avidin was either added externally or was pre-encapsulated in vesicles to assess the fraction of liposome-bound biotinylated GALA that exposed its labeled terminus to the external or internal side of the bilayer, respectively. Under conditions where most of the membrane-bound peptides were involved in transmembrane aggregates and formed aqueous pores (at a lipid/bound peptide molar ratio of 2500/1), the head-to-tail (N- to C-terminus) orientation of the membrane-inserted peptides was such that 3/4 of the peptides exposed their N-terminus on the inside of the vesicle and their C-terminus on the outside. Under conditions resulting in reduced pore formation (at higher lipid/peptide molar ratios), we observed an increase in the fraction of GALA termini exposed to the outside of the vesicle. These results are consistent with a model (Parente et al., Biochemistry, 29:8720, 1990) that requires a critical number of peptides (M) in an aggregate to form a transbilayer structure. When the peptides form an aggregate of size i, with i < M = 4 to 6, the orientation of the peptides is mostly parallel to the membrane surface, such that both termini of the biotinylated peptide are exposed to external BODIPY-avidin. This BODIPY-avidin/biotin binding assay should be useful to determine the orientation of other membrane-interacting molecules.  (+info)

Chemical cleavage of the overexpressed mitochondrial F1beta precursor with CNBr: a new strategy to construct an import-competent preprotein. (7/556)

We have isolated a soluble import-competent 15 kDa N-terminal fragment of the overexpressed Nicotiana plumbaginifolia F1beta precursor of the ATP synthase (N15pF1beta). The isolation was achieved after chemical cleavage, with CNBr, of the insoluble precursor collected in inclusion bodies, followed by purification of the fragment using ion-exchange chromatography. The purity of the final product was estimated to be more than 99%. N15pF1beta contained a presequence of 54 amino acid residues (except for the N-terminal methionine residue) and 82 N-terminal residues of the mature protein. N15pF1beta was shown to be imported into isolated potato tuber mitochondria and to be processed by the isolated mitochondrial processing peptidase (MPP) integrated into the cytochrome bc1 complex of the respiratory chain. Addition of N15pF1beta at micromolar concentrations resulted in the inhibition of import of F1beta precursor and alternative oxidase precursor, synthesized in vitro, into isolated mitochondria as well as the processing of these precursors catalysed by the isolated MPP-bc1 complex. N15pF1beta conjugated via a biotin link to avidin blocked import sites even after the reisolation of mitochondria and inhibited the import of the mitochondrial precursors, indicating that it can be used as a substrate for the generation of a stable translocation intermediate. Our results present a novel procedure for the production of an N-terminal fragment of the F1beta precursor that contains all information necessary for mitochondrial targeting and processing and that can be used for structural and functional studies of the mitochondrial protein import system. This procedure has a general value because it can be used for the production of chemical quantities of any mitochondrial import substrate and presequence peptide.  (+info)

Tumor pretargeting with avidin improves the therapeutic index of biotinylated tumor necrosis factor alpha in mouse models. (8/556)

The clinical use of tumor necrosis factor alpha (TNF) as an anticancer drug is limited to local or locoregional administration because of dose-limiting systemic toxicity. We investigated in animal models whether the therapeutic index of systemically administered human or murine TNF can be increased by tumor pretargeting strategies based on the biotin-avidin system. Pretargeting of s.c. mouse WEHI-164 fibrosarcoma and RMA lymphoma genetically engineered to express the Thy 1.1 antigen on the cell membrane was achieved by i.p. injection of a biotinylated anti-Thy 1.1 antibody and avidin. This pretreatment increased the antitumor activity of systemically administered biotin-TNF conjugates by at least 5-fold. In contrast, pretargeting did not increase the toxicity of biotin-TNF, as judged by animal survival and weight loss after treatment. Ex vivo analysis of tumor cells 24 h after treatment showed that biotin-TNF persisted for several hours on the surface of pretargeted tumors, but not when avidin was omitted. The potentiation of the antitumor effects was related primarily to indirect mechanisms, involving a host-mediated response. The results indicate that tumor pretargeting improves the antitumor activity of TNF. Tumor pretargeting with avidin, which is currently used to increase the uptake of radioactive-labeled biotin in patients, could represent a new strategy for improving the therapeutic index of TNF.  (+info)

Avidin fed to A. pisum was found to bind to the stomach region of the gut after ingestion and was retained for at least 72 hours. Feeding conjugates of avidin with fluorescently labelled biotin, or a fluorescently labelled, biotinylated peptide, showed that the conjugated compound was also retained in the aphid gut after feeding. A conjugate between avidin and biotinylated leucomyosuppressin (LMS), a myoinhibitory peptide hormone that affects gut contractions in insects, was prepared and fed to aphids. The avidin : biotin-LMS conjugate had insecticidal activity towards A. pisum when fed in diet at levels which neither of the components (avidin or biotin-LMS) caused significant mortality. It was hypothesised that binding to the gut, through the avidin moiety, was responsible for the observed oral toxicity of the avidin : biotin-LMS conjugate. The same principle was applied to lepidopteran larvae. A conjugate between avidin and biotinylated allatostatin was prepared and fed to M. brassicae larvae. ...
Avidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and specificity consisting of 128 amino acids and has a molecular mass of about 67K Daltons. Avidin-Biotin is a modified form of affinity purified avidin that combines the high affinity specific activity of the native egg white avidin to biotin molecule together with a very low background of the streptavidin produced by the bacteria Streptomyces avidinii.
Although thoroughly studied in terms of structural, biochemical and biophysical characteristics, the biological functions of avidins are not fully understood. Here, we expand the research on avidins by reporting xenavidin, the first avidin-like protein from a frog, which was identified as an expressed sequence tag (EST) from the Xenopus tropicalis genome project. Avidins appear to be conserved among egg-laying vertebrates [3, 4, 6] and are thought to have a role as defence proteins against microbial infections [43, 44]. Bird eggs contain egg yolk, the compartment for embryonic development, and the egg white that surrounds the egg yolk provides both nutrients and protection for the embryo. In frogs, the equivalent to egg white is called egg jelly [45]. The avidin content of egg jelly may have an important role in the embryonic development of frogs, a hypothesis that is also supported by recent studies within natural bird populations, in which the concentration of avidin in egg white appears to be ...
Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis.. Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA.. Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin ...
The Fluorescent Avidin Kit contains 500g of three fluorescent avidin products: Fluorescein Avidin DCS, Texas Red Avidin D and AMCA Avidin D.
One of the most popular methods of noncovalent conjugation is to make use of the natural strong binding of avidin or its derivative streptavidin to biotin. Each avidin molecule contains a maximum of four biotin binding sites thereby increasing the strength of their interaction with biotin. Depending on the functionality present on the biotinylation compounds, specific reactive groups on antibodies may be modified to create a avidin binding site. Amines, carboxylates, sulfhydryls, and carbohydrate groups can be specifically targeted for biotinylation through the appropriate choice of biotin derivative.. Avidin is a glycoprotein found in egg whites that contains four identical subunits of 16,400 Da each. The subunits each contain one binding site for biotin also known as vitamin H. The biotin interaction with avidin is the strongest noncovalent affinity known, exhibiting a dissociation constant of about 1.3×10-15M. Tryptophan and lysine residues in each subunit are involved in forming the binding ...
The avidin-biotin system is a technique for studying the interaction between two biomolecules in an indirect manner, as follows: Biotin is chemically coupled to a binder molecule (e.g., a protein, DNA, hormone, etc.) without disturbing the interaction with its target molecule; avidin is then used to sandwich between the biotinylated binder and a reporter molecule or probe. This allows for a variety of tasks, including localization and identification of the binder or target molecule. Consequently, the avidin-biotin system can frequently replace radioactive probes. Together with Ed Bayer, Wilchek established the Avidin-biotin system as a powerful tool in biological sciences. Early in the 1970s, they exploited Avidin as a probe and developed new methods and reagents to biotinylate antibodies and other biomolecules. Today, the system is applied in research and diagnostics as well as medical devices and pharmaceuticals. Examples include western blot, ELISA, ELISPOT and pull-down assays.[12] More ...
Biotin Agarose Resin is used for purification or removal of avidin or streptavidin samples. Biotin is immobilized through a spacer arm by means of covalent binding that minimize leakage. The binding is very strong, making it suitable for non-reversible b
Immunohistochemistry protocol - Avidin/Biotin Method (ABC). The principle of ABC detection kits is based on the affinity of avidin (or streptavidin) for biotin...
Fingerprint Dive into the research topics of Electrophoretic layer-by-layer assembly of biotin/avidin functionalized nanoparticles. Together they form a unique fingerprint. ...
Abstract The avidin-biotin-peroxidase complex enzyme-linked immunosorbent assay (ABC-ELISA) and standard ELISA were used for the detection of Echinococcus granulosus antibody in sera of 101 patients operated on for hydatid disease, 40 patients with miscellaneous nonhydatid diseases, and 61 normal subjects. Sensitivity and specificity of the two procedures were comparable and the geometric mean antibody titer detected with ABC-ELISA was higher than with standard ELISA. The ABC-ELISA is a sensitive, specific, simple, and convenient method for diagnosing hydatidosis.
The ABC method is an indirect method of immunohistochemistry. It stands for the Avidin-Biotin Complex Method. Firstly, the tissue of interest is sectioned and is incubated with a primary antiserum that targets the antigen we would like to locate in the tissue section. An antiserum is a serum containing antibodies such as agglutinins and antitoxins. This antiserum is known as the primary antibody and is injected into the tissue to target the antigen and causes an antibody-antigen reaction. After the primary antibody is inserted. A secondary labeled antibody is added, namely a biotinylated antibody. The secondary antibody reacts with the first antibody and launches a large mass of biotin into the area in which the antigen is situated. The secondary antibody is reacts in an opposite manner to the primary antibody. The secondary antibody does not have the intention in reacting with the antigen. The addition of the avidin biotin enzyme complex causes binding to the secondary antibody. The avidin ...
Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
1. The reaction between avidin and biotin was found to be exothermic, DeltaH being -20.3kcal./mole of biotin bound. The corresponding value of DeltaH for streptavidin was -23kcal./mole. 2. The heat evolved was independent of the pH (between 5 and 9), of the buffer (borate or ammonia) and of the fractional saturation of the avidin with biotin. 3. The entropy change for the reaction was zero, and it is suggested that the entropy increase to be expected from hydrophobic interactions was counterbalanced by a decrease in entropy accompanying the formation of buried hydrogen bonds. 4. Modification of the potential hydrogen-bonding sites of the imidazolidone ring led to a decreased heat output and a positive entropy of reaction.. ...
Abcam provides specific protocols for Endogenous Avidin + Biotin Blocking System (ab3387) : Endogenous Avidin + Biotin Blocking System
Avidin Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibians. It contains four identical subunits having a combined
In biochemistry, biotinylation is the entire process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is quick, certain and is not likely to perturb the natural operate from the molecule due to the small dimension of biotin (MW = 244.31 g/mol). Biotin binds to streptavidin and avidin with an especially superior affinity, quick on-price, and higher specificity, and these interactions are exploited in many areas of biotechnology to isolate biotinylated molecules of desire. Biotin-binding to streptavidin and avidin is resistant to extremes of heat, pH and proteolysis, earning capture of biotinylated molecules attainable in numerous types of environments ...
2000 Da) were able to fill all three of the remaining avidin-binding sites, while only one molecule of biotinylated PEG5000 or stem cell factor bound to each avidin. The resulting biotin−avidin−biotin linkages were stable for prolonged periods under continuous perfusion, even in the presence of excess free biotin. Hematopoietic M07e cells bound to immobilized peptide ligands for α5β1 (cyclic RGD) and α4β1 (cylic LDV) integrins in a DPB-dose-dependent manner, with near-maximal binding to cylic LDV for surfaces containing 1% DPB. Multiple ligands were adsorbed in a controlled manner by incubating NeutrAvidin with the respective ligands in the desired molar ratio and then adding the resulting complexes to DPB-containing surfaces. Cell adhesion to surfaces containing both cylic LDV and cyclic RGD increased in an additive manner compared to that for the individual ligands. The bioactivity of adsorbed biotinylated stem cell factor was retained, as demonstrated by DPB-dose-dependent M07e cell ...
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In the biotin-avidin method, cells are incubated first with biotin-conjugated monoclonal antibody and then subsequently incubated with fluorochrome-conjugated avidin. In the second antibody method, cells are incubated with an unconjugated monoclonal antibody followed by fluorochrome-conjugated goat anti-mouse Ig.. These sandwich techniques provide increased fluorescence intensity, which is valuable for microscopy. However, indirect methods can result in the formation of complexes that will artifactually stain Fc receptor-binding cells. The biotin-avidin method gives the least amount of artifactual staining particularly when streptavidin conjugates are used. Indirect methods will alter the proportionality between the amount of antigen and the fluorescence intensity per cell, therefore, these methods are not recommended for assessing the absolute number of antigenic determinants per cell. However, indirect methods can be used to determine the relative brightness differences between cell ...
Adenoviral vectors have great potential for use in gene therapy and genetic immunization. The targeting of Ad vectors to the relevant tissue and cell types in vivo could greatly improve their safety and performance by lowering the effective dosage required for therapeutic levels of gene expression. Redirection of Ad vector tropism will require physical modifications of the adenoviral capsid but direct genetic modification of the Ad capsid has so far been limited to small peptides. A novel system for the attachment of targeting ligands to the Ad capsid, based on the extremely strong avidin-biotin interaction, is described herein. The genetic insertion of a biotin acceptor peptide (BAP) into the fiber, protein IX, or hexon components of the Ad capsid has resulted in vectors that are metabolically biotinylated upon production in host cells. Avidin-dependent redirection of transduction through a variety of biotinylated ligands is greatly dependent on the nature of the biotinylated capsid protein. ...
TY - JOUR. T1 - An avidin-based assay for histone debiotinylase activity in human cell nuclei. AU - Chew, Yap Ching. AU - Sarath, Gautam. AU - Zempleni, Janos. PY - 2007/7/1. Y1 - 2007/7/1. N2 - The covalent binding of biotin to histones participates in heterochromatin formation, cell cycle progression and cellular response to DNA breaks. Biotinylation of histones appears to be a reversible process, but the identity of enzymes that remove biotin marks is largely unknown. Our long-term goal is to identify histone debiotinylases in human cells. Here we developed an avidin-based plate assay to quantify histone debiotinylase activities in nuclear extracts. This assay is an essential first step in purifying and identifying histone debiotinylases from human cells. Using this assay, we demonstrated that debiotinylation of histones depends on temperature and pH, consistent with enzyme catalysis. Experiments with purified histones, proteases and protease inhibitors provide evidence that removal of biotin ...
TY - JOUR. T1 - An equilibrium model of endothelial cell adhesion via integrin-dependent and integrin-independent ligands. AU - Chan, Bernard P.. AU - Bhat, Vinayak D.. AU - Yegnasubramanian, Srinivasan. AU - Reichert, William M.. AU - Truskey, George A.. PY - 1999/12/1. Y1 - 1999/12/1. N2 - Endothelial cell adhesion can be enhanced by supplementing integrin-mediated adhesion via fibronectin with the high-affinity avidin-biotin system in which biotin is covalently linked to membrane proteins and avidin binds to biotinylated surfaces (Bhat et al. J Biomed Mater Res 1998;41:377-85). An equilibrium model was extended to explain detachment of spreading cells following exposure to flow for this two ligand system. The two different receptor-ligand systems were treated as springs in parallel in which the equilibrium dissociation constant was a function of the separation distance of the cell from the surface. Flow experiments were performed to measure the endothelial cell adhesion strength as a function ...
TY - JOUR. T1 - Pilot therapy trial of CEA positive tumours using a three-step pretargeting approach. AU - Paganelli, G.. AU - Magnani, P.. AU - Chinol, M.. AU - Sudati, F.. AU - Zito, F.. AU - Mangili, F.. AU - Li, M.. AU - Meares, C. F.. AU - Siccardi, A. G.. AU - Fazio, F.. PY - 1998. Y1 - 1998. N2 - The three-step pretargeting approach, based on the avidin-biotin system, has been shown to improve the target/non target ratio in radioimmunoscintigraphy. This pilot study was designed to assess the feasibility of applying the same strategy to radioimmunotherapy. Five patients, with advanced metastatic CEA positive carcinomas were studied. All patients received two cycles of therapy with an interval of 3 months in between. The protocol consisted of an i.v. injection of 10 mg of biotinylated anti-CEA antibody, followed 24h later by avidin: 3 mg as rapid bolus (chase effect) plus 50 mg in 100 ml of saline. Biotin-LC-DOTA (2.5 mg) was labelled with Y-90 and injected i.v. 24 h later. The injected ...
Numerous spider toxins are of interest as tools for neurophysiological research or as lead molecules for the development of pharmaceuticals and insecticides. Direct detection and identification of the interacting proteins of a spider toxin are helpful for its action-mechanism analysis and practi...
Our family of biotin-binding proteins includes streptavidin, avidin, and NeutrAvidin® protein. See all conjugated and unconjugated streptavidin products.
Immunoelectron microscopy identifying avidin (8-nm gold) and cathepsin L (15-nm gold) immunoreactivity in organelles from late endosome, lysosome, and hybrid
Buy AVD recombinant protein, Avidin (AVD) Recombinant Protein-NP_990651.1 (MBS1122773) product datasheet at MyBioSource, Recombinant Proteins
Gentaur molecular products has all kinds of products like :search , MarkerGene \ Trifluoroacetamidoiminobiotin NHS ester, Reversibly binds to avidin preventing protein denaturation during purification, 10mg \ M1295 for more molecular products just contact us
Structural Characterization of the Avidin Interactions with Fluorescent Pyrene-Conjugates: 1-Biotinylpyrene and 1-Desthiobiotinylpyrene†. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
In the first part of this work, microfabricated Capillary Electrophoresis devices were integrated with microfabricated PCR devices for fast DNA amplification and separation., rapid on-chip amplification and separation of genes of interest was performed. A micro DNA extraction device using paramagnetic micro silica beads was consequently developed, subsequently a combined on-chip DNA extraction from a single drop of blood and PCR amplification were performed. In the second part of this work, micro array-based strategies for proteomics studies were developed. Two new strategies for site-specific immobilization of kinase substrates onto functionalized slides were developed: In addition, a novel fluorescent antibody-based detection was developed to rapidly screen for kinase substrate phosphorylation. Avidin-biotin is one of the strongest know non-covalent interaction, and by arraying C-terminal biotinylated proteins on avidin functionalized slides, a very stable array of functionally active proteins ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
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Although ovarian cancer implants can be targeted using an avidin-3 rhodamineX complex, there are several reasons to consider alternative complexes. GmSA-20ROX is substantially less immunogenic compared with avidin, and GmSA has been used as the basis for a technetium-99m-labeled radiopharmaceutical to assess hepatic reserve in humans. Additionally, GmSA-20ROX binds target cells more rapidly and more efficiently than avidin due to its multivalency (7, 16). Because GmSA synthesis involves the conjugation of 23 d-galactosamines reacted with carboxyl groups rather than amino groups on an albumin molecule (7), there are multiple binding sites for the d-galactose receptor and the molecule has a favorably high isoelectric point (7, 16). Moreover, activation via dequenching of the rhodamineX after cellular internalization provides a generalizable platform for targeted fluorescent probes by exchanging the targeting moiety (5). We used the d-galactose receptor as a target in this study because it is ...
Grade: Biotech Appearance Fine, white homogeneous powder Moisture content ≤10% Gel Strength 1.5% ≥1,120 g/cm2 Gelling Point 34.5 - 37.5°C Electroendosmosis-Mr 0.09-0.13 Sulfate ≤0.15% DNase , RNase and protease None detected ...
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
Fluorescent microscopy is a technique where a lower wavelength of light is applied to a sample, but only the light emitted at a higher wavelength is detected. Fluorescent microscopy allows for the sensitive visualization of fluorescent molecules against a dark background. Let Protein Mods label your primary antibody, so you can avoid wasting time with secondary antibodies. In addition to services, Protein Mods offers a number of proteins modified with fluorescein, tetramethylrhodamine, and CY5.5 for your fluorescent microscopy needs. Additional fluorophore options are available upon request. Anti-rabbit IgG Anti-mouse IgG Avidin Protein A Negative controls available in our catalog: Rabbit IgG Mouse IgG Canine IgG Rat IgG BSA
Rachel Teitelbaum wrote: , Hi Have you done a dilution experiment? Are you using a 2nd AB or a biotin avidin system? What is the enzyme read out or are you using fluorescence? Can you stain lymphocytes and pick up positives on a FACS ...
Tumour pretargeting is a promising strategy for cancer diagnosis and therapy allowing for the rational use of long circulating, highly specific monoclonal antibodies (mAbs) for both non-invasive cancer radioimmunodetection (RID) and radioimmunotherapy (RIT). In contrast to conventional RID/RIT where the radi
Labelling antibodies and proteins is easier than ever with SureLINK Protein Labelling Kits and Reagents! These fast, convenient, ready-to-use kits are sure to work every time whether you are labelling your proteins with biotin, enzymes, or fluroescein.. SureLINK AP Conjugation Kits and Reagents. These kits are based on novel conjugation chemistry far superior to conventional methods such as maleimide/thio, glutaraldehyde and avidin/biotin. Kits and modified enzymes are capable of conjugating a variety of proteins in a range of sizes.. SureLINK HRP Conjugation Kits and Reagents. These kits are based on the well-established periodate chemistry that yields consistent, reproducible antibody or protein conjugates. Horseradish peroxidase (HRP) is pre-activated and supplied in reaction size vials. Kits and enzymes are capable of conjugating a variety of proteins in a range of sizes.. SureLINK Chromophoric Biotin Labelling Kits. These kits provide an easy-to-use method for biotinylating antibodies and ...
Hi, One of my pathologists is asking if I can take a slide that has already been stained with a peroxidase/avidin/biotin/DAB detection system and re-stain with an Alkaline Phosphotase detection system. Can it work? Must it be de-stained? Any info will help. Thanks, Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ...
10-plate (plates NOT included). Monoclonal antibody to human interleukin 10 (IL-10). Mouse IgG|sub|1. Biotinylated monoclonal antibody to human interleukin 10 (IL-10).Mouse IgG|sub|1. The cytokine ELISPOT (Enzyme-Linked ImmunoSPOT) assay is designed to enumerate cytokine-secreting cells in single cell suspensions of lymphoid tissue, central nerve system (CNS) tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMCs). The assay has the advantage of detecting only activated/memory T cells and has the ability to detect cytokine release in response to antigen by a single cell thereby permitting direct calculation of responder T cell frequencies. The high sensitivity and easy performance, allowing the determination of peptide-reactive T cells without prior in vitro expansion, makes the ELISPOT assay eminently well suited to monitor T cell responses. The higher sensitivity of ELISPOT in comparison to that of ELISA or intracellular staining is due to the plate-bound antibodies directly
20-plate (plates NOT included). Monoclonal antibody to mouse interleukin 4 (IL-4). Rat IgG|sub|1. Biotinylated monoclonal antibody to mouse interleukin 4 (IL-4). Rat IgG|sub|1. The cytokine ELISPOT (Enzyme-Linked ImmunoSPOT) assay is designed to enumerate cytokine-secreting cells in single cell suspensions of lymphoid tissue, central nerve system (CNS) tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMCs). The assay has the advantage of detecting only activated/memory T cells and has the ability to detect cytokine release in response to antigen by a single cell thereby permitting direct calculation of responder T cell frequencies. The high sensitivity and easy performance, allowing the determination of peptide-reactive T cells without prior in vitro expansion, makes the ELISPOT assay eminently well suited to monitor T cell responses. The higher sensitivity of ELISPOT in comparison to that of ELISA or intracellular staining is due to the plate-bound antibodies directly
Non-covalent binding of avidin to biotinylated erythrocytes results in complement-dependent haemolysis. Biotinylated erythrocytes, as well as native cells, are not lysed by complement. Complement activation requires a tight contact between avidin and the erythrocyte membrane, since avidin does not in itself activate complement and does not inhibit lysis of sensitized sheep erythrocytes. The efficiency of haemolysis depends on avidins surface density. When the avidin concentration in the reaction mixture is less than 15 micrograms/ml, erythrocyte lysis is not induced. However, the attachment of biotinylated antibodies to avidin-carrying erythrocytes decreases dramatically. Acylation of avidin with succinic anhydride strongly decreases its ability to induce complement-dependent haemolysis. However, the ability of avidin to cross-link the biotin-containing structures decreases after acylation. A 50% modification of avidin by succinic anhydride (pI about 7.0) allows preparation of ...
Looking for online definition of avidin in the Medical Dictionary? avidin explanation free. What is avidin? Meaning of avidin medical term. What does avidin mean?
The avidin-biotin technology has many applications, including molecular detection; immobilization; protein purification; construction of supramolecular assemblies and artificial metalloenzymes. Here we present the recombinant expression of novel biotin-binding proteins from bacteria and the purification and characterization of a secreted burkavidin from the human pathogen Burkholderia pseudomallei. Expression of the native burkavidin in Escherichia coli led to periplasmic secretion and formation of a biotin-binding, thermostable, tetrameric protein containing an intra-monomeric disulphide bond. Burkavidin showed one main species as measured by isoelectric focusing, with lower isoelectric point (pI) than streptavidin. To exemplify the potential use of burkavidin in biotechnology, an artificial metalloenzyme was generated using this novel protein-scaffold and shown to exhibit enantioselectivity in a rhodium-catalysed hydrogenation reaction ...
Streptavidin is a protein that has similar binding properties to egg white avidin. It is isolated from streptomyces avidinii. Streptavidin has a molecular weight of 60 kDa and has 4 subunits. Each subunit can bind one molecule of biotin. Biotin is a water-soluble vitamin. Streptavidin has an extremely high binding affinity (Kd=10-15) for biotin. It has proven useful in the detection of antigens coupled with biotinylated secondary antibodies. There are several advantages when using streptavidin versus an avidin complex (ABC).In contrast to avidin, streptavidin is not glycosylated and is therefore uncharged at neutral pH (6.5 versus 10). This lowers nonspecific background staining. Streptavidin also lacks carbohydrate side chains that may be another cause of non-specific background. Another key advantage of streptavidin is the significant increase in sensitivity (probably due to less steric hindrance), thus facilitating an increase in overall binding capacity. Finally, streptavidin-enzyme conjugates are
A plethora of biomolecules different from nucleic acids or antibodies has also been coupled to OFGs for biosensing applications, ranging from the high affinity couple avidin-biotin, to enzymes and bacteriophages.. The main criterion of biosensing is the specific molecule recognition through a BRE, which selectively targets the desired analyte. In a series of studies, the pair biotin/streptavidin or avidin was used as a demonstrative bio-conjugate pair, which shows an extraordinarily high affinity (Kd≈10−14m, Kd≈10−15m, biotin/streptavidin and biotin/avidin, respectively). These kinds of biosensors are for the great majority characterized by the use of biotin as the BRE and the streptavidin as the analyte to be detected in buffer solutions. Marques et al. [60] developed a sensor based on an LPG modified with a coating of silica core gold shell (SiO2:Au) nanoparticles (NPs), which were deposited using the layer-by-layer method (poly(ally-lamine hydrochloride) (PAH) polycation layer), ...
A plethora of biomolecules different from nucleic acids or antibodies has also been coupled to OFGs for biosensing applications, ranging from the high affinity couple avidin-biotin, to enzymes and bacteriophages.. The main criterion of biosensing is the specific molecule recognition through a BRE, which selectively targets the desired analyte. In a series of studies, the pair biotin/streptavidin or avidin was used as a demonstrative bio-conjugate pair, which shows an extraordinarily high affinity (Kd≈10−14m, Kd≈10−15m, biotin/streptavidin and biotin/avidin, respectively). These kinds of biosensors are for the great majority characterized by the use of biotin as the BRE and the streptavidin as the analyte to be detected in buffer solutions. Marques et al. [60] developed a sensor based on an LPG modified with a coating of silica core gold shell (SiO2:Au) nanoparticles (NPs), which were deposited using the layer-by-layer method (poly(ally-lamine hydrochloride) (PAH) polycation layer), ...
OBJECTIVE: To analyze the role of immunochemistry in serous effusions.STUDY DESIGN: We analyzed cell blocks of 18 pleural and 18 peritoneal effusions diagnosed as malignant (18), benign (14) and suspicious (4). They were immunostained by the avidin-biotin complex method with a panel of four monoclonal antibodies-CEA, Ber-EP4, LeuM1 (CD15) and r53-and for lectins (Ulex europaeus) UEA-1, ConA and ConBr. RESULTS: Seventeen of the 18 cases of adenocarcinoma were positive for CEA (95%), 12 (66.6%)for Bev-EP4, 11 (61 %)for CD15 nild 11 (62 %)for p53. Twelve of the 18 (66.6%) were positive for UEA-1, CEA, Ber-EP4 and CD15. UEA-1 did not react with mesothelial cells. p53 Gave a positive reaction in only one case, reactive mesothelial cells. ConA and ConBr reacted indiscriminately with benign and malignant cells; thus, it was not useful in distinguishing between these cells.CONCLUSION: In this context no antibody used alone is reliable for corroborating a diagnosis, but the selective use of a small panel ...
So, who is Muscle Egg egg whites? Muscle Egg is a Family owned enterprise. We are a state of the art egg processing facility that has been providing the highest quality eggs and egg products for over 50 years. Muscle Egg Liquid Egg Whites are produced in a USDA compliant facility with state of the art equipment. This ensures the safest, highest quality liquid egg whites possible. Our egg whites are salmonella, listeria and avidin free. Avidin, found in raw eggs, affects B vitamin biotin, stops it being absorbed, and causes problems with fatty acid synthesis and blood sugar levels. The pasteurising process for the liquid egg whites removes the avidin, and any salmonella or listeria, but the egg whites remain liquid, ready for cooking or immediate consumption. ...
Fluorescent Dyes , Biotins and Streptavidins , Fluorescein biotin; Green fluorescent biotin used for studying avidin binding; C42H50 N6O8S2
In 2012, Roux et al. published a nice paper, that received no less than four article recommendations from F1000 researchers. The paper described a method for tracking the interaction partners a protein has had within a cell (a history of its interacting partners). The method, called BioID, is based on proximity-dependent biotinylation of proteins by a promiscuous biotin ligase mutant BirA (R118G), which is fused to your protein of interest. After an overnight incubation with biotin, cells can be subjected to harsh lysis and biotinylated proteins can be isolated and identified by mass spectroscopy to determine the proteins that had come into contact with the chimeric BirA (R118G) protein. This method is a bit different from standard co-IP or pull-down experiments, because it allows one to identify proteins who interact transiently or weakly with the protein of interest. Also, due to the strong biotin-avidin binding affinity, harsh washes can greatly reduce background protein binding. [Read ...
Isolated C7 (m.w. 120,000) in 1% deoxycholate (DOC) forms dimers with an apparent m.w. of 230,000 and a DOC-binding capacity of 82 mol per mol of dimer. Dimerization of C7 also occurs in the presence of DOC-phospholipid mixed micelles and eventuates in the insertion of C7 dimers into the lipid bilayer upon the removal of the detergent. C5b-7 complex formation in the fluid phase or on lipid vesicles likewise involves polymerization. C5b-7 sedimented with 17-40S, which suggests a dimeric to hexameric composition. In avidin-biotin binding experiments in which two differentially labeled forms of C5b,6 (biotinyl 125I-C5b,6, and 131I-C5b,6) were used in equimolar amounts to assemble C5b-7, more than 50% of the biotinyl 125I-C5b,6-containing complexes also contained 131I label; again suggesting that C5b-7 consisted of oligomers rather than monomers. The conformation of C7 in C5b-7 and in dimeric C7 appeared similar by the following criteria. On formation of C5b-7 from C5b,6 and C7, a 20% increase in ...
Arrayit microarray substrate and microarray slide products are polished to atomic smoothness and treated and coated with clean, amine, aldehyde, epoxy, mirror, avidin, streptavidin, gold, sputtered, nitrocellulose, and PVDF surface chemistries for superior performance in research, genomics, proteomics and diagnostics. Substrate slide dimensions are standard 25 x 76 x 1 mm open platform. Arrayit also offers custom microarray substrates and microarray slides to any dimension with laser etching, chrome fiducials, and custom glass formulations.
Biotin is an important molecule for modern biological studies including, e.g., cellular transport. Its exclusive affinity to fluorescent streptavidin/avidin proteins allows ready and specific detection. As a consequence methods for the attachment of biotin to various biological targets are of high importance, especially when they are very selective and can also proceed in water. One useful method is Hüisgen dipolar [3+2]-cycloaddition, commonly referred to as
Dear everone, I am a research asssitant working in Cincinnati Childrens Hospital Research Foundation. I am currently doing dual-label FISH (Fluorecent Insitu Hybridization). I choosed the following schema: 1. dig labeld mRNA probe of gene1---------mouse anti-dig(Roche)---------Goat anti mouse with HRP(Molecular Biology)------Tyramide Signal Amplification (Molecular Biology) 2. biotin labeld mRNA probe of gene2------------strepavidin-HRP (Jacson Immuno)------Tyramide Signal Amplification (Molecular Biology) The first one (dig) gave me nice staining, but the second one (strepavidin) always gave high background. I know the biotin labeled probe is good, since when I use this probe tested with mouse-anti-bio antibody, then followed by Goat-anti-mouse-HRP second antibody, it works beautifully. So, the problem could be biotin-avidin problem. Did anyone experience same problem when doing FISH? Appreciate it! Sincerely Yours, Ying Wen -------------- next part -------------- An HTML attachment was ...
Detecting ligand-receptor binding on cell membrane surfaces is required to understand their function and behavior. Detection platforms can also provide an avenue for the development of medical devices and sensor biotechnology. The use of fluorescence techniques for such purposes is highly desirable as they provide high sensitivity. Herein, we describe a technique that utilizes the sensitivity of fluorescence without directly tagging the analyte of interest to monitor ligand-receptor interactions on supported lipid bilayers. The fluorescence signal is modulated according to the charge state of the target analyte. The binding event elicits protonation or deprotonation of pH-responsive reporter dyes embedded in the lipid bilayer. Supported lipid membranes containing ortho-conjugated rhodamine B-POPE (1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine), which fluoresces in its protonated but not in its deprotonated form, were utilized as sensor platforms for biotin-avidin and biotin
For histological analyses, we have chosen the two major branches of the femoral nerve, the quadriceps muscle nerve, and the cutaneous saphenous nerve of the mouse. In some experiments, sciatic nerves have been investigated at the level of the sciatic notch. In addition, we investigated the spinal roots of the third and fourth lumbar segment.. For detection and quantification of T lymphocytes, antibodies to CD8 (a gift from R. Zinkernagel, University of Zürich, Zürich, Switzerland) have been used on serial nerve cryosections as described previously (Schmid et al. 2000).. For detection of macrophages, antibodies to mouse F4/80 (1:300; Serotec) were applied overnight on 14-μm-thick serial sections from fresh frozen femoral nerves and spinal roots. To visualize primary antibodies, a biotinylated secondary antibody to rat Igs was applied for 1 h, followed by avidin/biotin reagent (Dako) and staining with diaminobenzidine-HCl and H2O2. For negative control, the primary antibody was omitted. In ...
Representative HSP-70-immunostained lung sections obtained following administration of AdGFP or AdHSP. C48 indicates 2CLP with virus administration; tissue was fixed 48 hours afterward. Primary antibody was polyclonal goat anti-rat HSP-70; secondary antibody was rabbit anti-goat IgG conjugated to horseradish peroxidase. Detection was performed with immunoperoxidase/avidin/biotin and metal-enhanced 3,3-diamino-benzidine. Arrows indicate HSP-70 staining, primarily in type II pulmonary epithelial cells ...
Surface biotinylation and NeutrAvidin pull-down were performed similar to what has been described previously (Zha et al., 2009a; Jing et al., 2011). For biotinylation of organotypic hippocampal slices, each filter, which contained 6-8 slices, was cut out and put in a six-well plate. CHO cells or slices were then washed three times with ice-cold PBS+/+, followed by 30 min incubation at 4°C in 1.5 ml (for CHO cells in 60 mm dishes) or 1 ml (for slices in each well of the 6-well plate) of PBS+/+ containing 0.5 mg/ml Sulfo-NHS-LC-Biotin. Cells were washed once with cold PBS+/+ and the reaction was quenched by 100 mm glycine in PBS+/+. Of note, it is essential, especially for biotinylation of slices, to keep the solution and plates ice cold during the whole procedure. Cells were lysed in 300 μl of NeutrAvidin lysis buffer (PBS, 1% Triton, 0.5% SDS, 0.5 mg/ml N-ethylmaleimide, with protease inhibiters). Cell lysates were sonicated briefly and centrifuged at full speed with a desktop centrifuge for ...
Biotin-labeled oligonucleotides have become extremely popular in DNA capture and detection applications, due to their very high sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotin-based detection methods are widely used by researchers. Biotin labeling is compatible with PCR and most hybridization techniques.Key Features of the Biotin Phosphoramidite Behaves like any standard amidite on the DNA synthesizer Readily soluble in acetonitrile Biotin compound has a dimethozytrityl (DMT) - group on the ring structure, which allows coupling-yield determination and trityl-selective purification A long, water-compatible linker arm ensures maximum sensitivity The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purification is required Provides a coupling efficiency of 95%
Due to the immobilization of the capture enzyme HRP in close proximity to the marker enzyme (GOD), more intense and specific staining is produced than can be obtained with soluble HRP as coupling enzyme in the substrate medium. Indirect antibody labelled and antibody bridge techniques including the avidin (streptavidin)-biotin principle have proven the usefulness of this GOD labelling procedure for antigen localization in paraffin sections. Antigens such as IgA in tonsil, alpha-fetoprotein in liver and tissue polypeptide antigen in mammary gland served as models ...
Catalytic colloidal metal particles bound to a biomolecule such as an antibody, avidin, or streptavidin are useful for detecting the presence of the biomolecule in an assay such as an immunoassay.
Zen Cart! : Mouse IgM (mu) - Primary Antibodies Secondary Antibodies Protein A & G Kits IQFCS Avidin/Streptavidin Magnetic Beads Biochemical Reagents Services & Technology ecommerce, open source, shop, online shopping
Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound.
Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound.
ImmunoCruz trade rabbit ABC Staining System utilizes avidin and biotinylated horseradish peroxidase ABC reagents. Sufficient for 200 slides.
Lakkey, HV and Rao, AGA and Prakash, V and Krishnaswamy, PR and Savithri, HS and Rao, NA and Ramadoss, CS (1999) Affinity properties of phosvitin: Interaction of phosvitin with serine hydroxymethyl transferase. In: Indian Journal Of Biochemistry & Biophysics, 36 (2). pp. 69-76. Shenai, BR and Komalam, BL and Arvind, AS and Krishnaswamy, PR and Rao, Subba PV (1996) Recombinant antigen-based avidin-biotin microtiter enzyme-linked immunosorbent assay for serodiagnosis of invasive amebiasis. In: Journal of Clinical Microbiology, 34 (4). pp. 828-833. Nagpal, S and Sriramarao, P and Krishnaswamy, PR and Metcalfe, DD and Rao, PV (1990) Demonstration of IgE Antibodies to Nucleic Acid Antigens in Patients with Sle. In: Autoimmunity, 8 (1). pp. 59-64. ...
Chemistry, Biophysics, Peptide, Biomolecule, Protein engineering, Supramolecular chemistry, Nanotechnology, Biosensor, Avidin, Linker,
Eyes removed from normal or immunized animals at the various stages of EAU were embedded in optimal cutting temperature (OCT) compound, snap frozen, and stored at −30°C. Serial 8-μm cryostat sections of the eyes were taken onto poly(l-lysine)-coated slides and air dried overnight. Cells were isolated from diseased eyes at various phases of disease and cytospins made with ∼5 × 105 cells per slide. Before staining, tissue sections or cytospins were fixed in an acetone-methanol (1:1) mix at −20°C for 5 to 10 minutes and air dried. Sections were stained using either single or dual fluorescence for the following Abs: nitric oxide synthase (NOS)-2 (Clone 6, 1:100; Transduction Laboratories, Nottingham, UK) and infiltrating blood-borne macrophage marker ED1 (1:50, FITC conjugate; Serotec), and also through a standard avidin-biotin (alkaline phosphatase) technique for ED1 (1:100, purified, Serotec). The number of ED1-positive cells infiltrating throughout the retina and subretinal space, were ...
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Mishi i pabërë: Shmangni kategorikisht mishin e pagatuar gjatë shtatzënisë pasi ai rrit mundësinë e prekjes nga toksoplasmosa, një infeksion i krijuar nga paraziti me të njëjtin emër.. Djathërat e butë dhe gjysmë të butë: Kërkojmë ndjesë nëna, por gjatë kësaj faze nuk mund të konsumoni djathë Gorgonzola dhe Camembert pasi rrezikoni të prekeni nga listerioza.. Qumështi jo pasterizuar: Qumështi është shumë i rëndësishëm në regjimin e një gruaje shtatzënë, por vetëm ai i pasterizuar. Qumështi i papasterizuar përmban agjentë patogjenë të dëmshëm.. Vezët e gjalla: Shmangni konsumin e vezëve të gjalla pasi e bardha e vezës përmban avidinë, një substancë që pengon përhtithjen e vitaminës H.. Pijet alkolike: E dimë që ju tundon një gëllënjkë verë ose një kokteil me alkolin tuaj të preferuar, por duhet të prisni deri në fund të nëntë-mujorit. Konsumi i pijeve alkoolike mund të dëmtojë zhvillimin fizik të fetusit.. Coca- Cola: ...
Vitamin H (D-biotin) is a white to off-white crystalline powder. It is important for the degeneration of amino and fatty acids. Applications for Vitamin...
EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.,This kit is designed to select cells labeled with biotinylated antibodies. Kit includes reagents for labeling 3 x 10 cells and EasySep magnet.,biological,biology supply,biology supplies,biology product
Avoid the delays associated with coordinating antibody sample shipment and testing between another service provider. The assay development team at MBS can compress project timelines by designing and performing additional assays to more thoroughly characterize the hybridoma candidates in alignment with the known project goals. With our high-throughput MultiPure process, a library of purified and biotinylated antibody samples is available to support matched pair compatibility screening for sandwich ELISA applications. Antibody pairs can be assessed for sensitivity and evaluated for specificity against cross-reactants of concern. ...
Company profile & key executives for Haba West Sales & Marketing (4886579Z:-) including description, corporate address, management team and contact info.
Does anyone experience this? Whenever I get the flu it doesnt affect my bgs at all and when its a bacterial infection requiring antibiotics (like strept throat), my bgs immediately shoot to over 400 and I know that I need antibiotics to bring it down. As soon as I take them, it goes down in a few hours. I also have found that if I am getting strept throat, I can take 3 echinachea (sp?) and it makes my bgs come down, too. Personally, I prefer taking the herbs so I dont have to go to the Dr. :) Jenie _________________________________________________________________ Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp ---------------------------------------------------------- for HELP or to subscribe/unsubscribe, contact: [email protected] send a DONATION http://www.Insulin-Pumpers.org/donate.shtml ...
Each set comes with six, themed puzzles, a matching puzzle piece for each of the six puzzles for cognitive associative development and then a wooden figure for imaginative story play. The chunky, high glass, soft snap cardboard puzzle pieces make them easy enough for a 1 ½ year old to complete all by themselves. Three styles available: Farm, Animals, Construction ...
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Biotin is a B vitamin which helps the body convert food into energy and plays many other important roles in health. Find out about the benefits of biotin here.
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Biotin helps your body produce energy from carbohydrate, proteins, and fats. Learn about food sources of biotin & problems associated with deficiency
Green, NM (1975). "Avidin". Advances in Protein Chemistry. 29: 85-133. doi:10.1016/S0065-3233(08)60411-8. ISBN 9780120342297. ...
Green NM (1975). "Avidin". Advances in Protein Chemistry. 29: 85-133. doi:10.1016/s0065-3233(08)60411-8. PMID 237414. Holmberg ...
Avidin is the other most notable biotin-binding protein. Originally isolated from egg yolk, avidin only has 30% sequence ... Avidin has a higher affinity for biotin (Kd ~ 10−15M) but in contrast to streptavidin, avidin is glycosylated, positively ... it has the advantage of much lower nonspecific binding than avidin. Deglycosylated avidin (NeutrAvidin) is more comparable to ... On the other hand, streptavidin is the better biotin-conjugate binder; avidin has a lower binding affinity than streptavidin ...
Avidin biotin media is used in the purification process of biotin/avidin and their derivatives. ...
"Meeduma Wage Avidin muhurath ceremony". Sarasaviya. Retrieved 24 August 2019. "The Journey of Sri Lanka's iconic entertainer ... Nisalai Hathwana Kandayama Ira Awara Isuru Pawura Kalu Kumari Kasee Salu Kinduru Adaviya Mahathala Hatana Meeduma Wage Avidin ...
The avidin-biotin system[edit]. The avidin-biotin system is a technique for studying the interaction between two biomolecules ... and the avidin-biotin system. The Avidin-biotin complex is the highest affinity interaction in nature, and its utilization to ... "Three-dimensional structures of avidin and the avidin-biotin complex". Proc. Natl. Acad. Sci. 90 (11): 5076-5080. Bibcode: ... The studies have culminated in the determination of the 3D structure of the avidin-biotin complex by X-ray crystallography,[13] ...
Biotinylated plasmid DNA was bound by avidin. New protein was synthesized by using cell-free expression system i.e. rabbit ...
"Avidin-Biotin Interaction - CA". www.thermofisher.com. Retrieved 2019-03-01. Kozubek S, Lukásová E, Amrichová J, Kozubek M, ...
"Components of Avidin-Biotin Technology: A Handbook." Pierce Biotechnology, Inc. Rockford, IL: June, 2003. "Photobiotin acetate ... Dontha, Narasaiah; Nowall, Wilbur B.; Kuhr, Werner G. (1997). "Generation of Biotin/Avidin/Enzyme Nanostructures with Maskless ...
Michael Green, N. (1990), "[5] Avidin and streptavidin", Avidin-Biotin Technology, Methods in Enzymology, 184, Elsevier, pp. 51 ... After the discovery of the fast interaction between Biotin and (Strept)Avidin, which show a high binding affinity, this ... Hnatowich, D. J.; Virzi, F.; Rusckowski, M. (August 1987). "Investigations of avidin and biotin for imaging applications". ...
"Immobilized nitro-avidin and nitro-streptavidin as reusable affinity matrices for application in avidin-biotin technology". ... Because egg-derived avidin binds strongly to biotin with a dissociation constant Kd of ≈10−8 M, biotinylated compounds of ... Second, the sample is incubated with avidin bound to beads, then rinsed, removing all unbound proteins while leaving only the ... Gyorgy P, Rose CS (1943). "The Liberation of Biotin from the Avidin-Biotin Complex (AB)". Experimental Biology and Medicine. 53 ...
"Organelle pH studies using targeted avidin and fluorescein-biotin". Chemistry & Biology. 7 (3): 197-209. doi:10.1016/S1074-5521 ...
"Artificial metalloenzymes for enantioselective catalysis based on biotin-avidin". Journal of the American Chemical Society. 125 ...
A new avidin-biotin ELISA assay for its measurement". Journal of Immunoassay. 13 (1): 47-60. doi:10.1080/15321819208019824. ...
The biotin-avidin system quickly became a central method in cell biology, immunology, and protein engineering, as well as ... Heitzmann H, Richards FM (1974). "Use of the avidin-biotin complex for specific staining of biological membranes in electron ... Diamandis EP, Christopoulos TK (1991). "The biotin-(strept)avidin system: principles and applications in biotechnology". ... new types of chemical tags such as biotin/avidin, the nuclear magnetic resonance (NMR) chemical shift index, and structural and ...
Gyorgy, P.; Rose, C. S. (1943). "The Liberation of Biotin from the Avidin-Biotin Complex (AB)". Experimental Biology and ... work on biotin and in 1941 published a paper demonstrating that egg-white injury was caused by the binding of biotin by avidin ...
"Adoptive immunotherapy by avidin-driven cytotoxic T lymphocyte-tumor bridging". Cancer Research. 60 (15): 4211-5. PMID 10945632 ...
Livnah, O.; Bayer, E.; Wilchek, M.; Sussman, J. (1993). "Three-dimensional structures of avidin and the avidin-biotin complex ... Biotin and avidin bind with a dissociation constant of roughly 10−15 M = 1 fM = 0.000001 nM.Ribonuclease inhibitor proteins may ...
CS1 maint: discouraged parameter (link) "Sudu Andagena Kalu Avidin in Rupavahini". Sarasaviya (in Sinhala). 21 March 2019. ... "Sudu Andagena Kalu Avidin in sri lanka Rupavahini Corporation" (in Sinhala). Mawbima. 23 March 2019. Retrieved 23 March 2019. ...
CS1 maint: discouraged parameter (link) "Sudu Andagena Kalu Avidin in sri lanka Rupavahini Corporation" (in Sinhala). Mawbima. ... CS1 maint: discouraged parameter (link) "Sudu Andagena Kalu Avidin in Rupavahini". Sarasaviya (in Sinhala). 21 March 2019. ...
"Sudu Andagena Kalu Avidin in sri lanka Rupavahini Corporation" (in Sinhala). Mawbima. 23 March 2019. Retrieved 23 March 2019. " ...
"Sudu Andagena Kalu Avidin in sri lanka Rupavahini Corporation" (in Sinhala). Mawbima. 23 March 2019. Retrieved 23 March 2019. " ...
CS1 maint: discouraged parameter (link) "Sudu Andagena Kalu Avidin in Rupavahini". Sarasaviya (in Sinhala). 21 March 2019. ...
She acted in a negative role in the drama Oba Ayemath Avidin. Anuradha was represented as a judge in many reality shows such as ... Maha Polowa Ape Newei Oba Ayemath Avidin Pinibara Yamaya Sakisanda Suwaris Wahinna Muthu Wessak Her acting career made a ...
The avidin-peroxidase labeling TUNEL assay is applicable for light absorption microscopy. Many TUNEL-related kits are ...
Avidin is an antinutrient found in active form in raw egg whites. It binds very tightly to biotin (vitamin B7) and can cause ...
Previously, a biotin-avidin magnetic bead system was used for this purpose. BioTechniques. 1998 Sep;25(3):516-21. Mutation ...
When cooked, avidin is partially denatured and binding to biotin is reduced. However one study showed that 30-40% of the avidin ... Egg whites contain high levels of avidin, a protein that binds biotin strongly. ...
He had leading roles in the discovery of folic acid, pantothenic acid, vitamin B6, lipoic acid, and avidin. He was elected to ... He also worked on discovering and isolating vitamin B6, lipoic acid, and avidin. Throughout his career Williams was a prolific ...
The monomeric avidin is created by treatment of immobilized native avidin with urea or guanidine HCl (6-8 M), giving it a lower ... Dimeric members of the avidin family are also found in some bacteria. In chicken egg white, avidin makes up approximately 0.05 ... A 1991 assay for the Journal of Food Science detected substantial avidin activity in cooked egg white: "mean residual avidin ... Aware of the strength and specificity of the avidin-biotin complex, researchers began to exploit chicken avidin and ...
Structures: Avidin/streptavidin (IPR005468). PDBe. The Protein Data Bank (PDB) is a repository for the 3-D structural data of ...
Egg-White Avidin in its functional complex with Biotin Avidin is a glycoprotein made of four identical subunits. Biotin is a ... Two Avidin chains are contained in an asymmetric unit. Avidins binding with biotin is one of the tightest binding ... The avidin-biotin complex is extremely tight and stable, the complex is stable in 9 M Urea. Tryptophan residues 70, 97 and 110 ... In each chain of avidin, when biotin is bound it is in close contact with Trp 70 and Trp 97 ...
... is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibians. It contains four ... Relationship between avidin and biotin Avidin has a very strong affinity for biotin with a KD (dissociation constant) of ... Carbohydrate accounts for about 10% of the total mass of avidin. Avidin has a basic isoelectric point (pI) of 10-10.5 and is ... Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibians. It contains four identical ...
The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of ...
Biotin-(strept)avidin complex is widely used in biotechnology because of its extremely high binding constant, but there is no ... Biotin-(strept)avidin complex is widely used in biotechnology because of its extremely high binding constant, but there is no ... avidin, but recover native affinity after UV irradiation. For application at the cell surface, we introduced an amine-reactive ...
Avidin is a glycosylated biotin-binding protein that has a basic isoelectric point and is widely used to detect biotinylated ... Our avidin, fluorescein conjugate has excitation and emission maxima ~494/518. ... Our avidin, fluorescein conjugate has excitation and emission maxima ~494/518. Avidin is a glycosylated biotin-binding protein ...
ALKALINE PHOSPHATASE-AVIDIN. (Avidin-AP). This conjugate is prepared using avidin from egg white. It is provided lyophilized ... AVIDIN-HRP. Liquid in 0.02M PBS, pH 7.3, with 10% glycerol and 0.01% thimerosal.. ...
... Ying Wen wenvt5 at yahoo.com Sat Dec 17 09:35:42 EST 2005 * ... So, the problem could be biotin-avidin problem. Did anyone experience same problem when doing FISH? Appreciate it! Sincerely ...
Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin). ... The biological function of avidin is not known. ... IPR005469 Avidin. IPR017889 Avidin-like_CS. IPR036896 Avidin- ... IPR005469 Avidin. IPR017889 Avidin-like_CS. IPR036896 Avidin-like_sf. IPR005468 Avidin/str. ... "Egg white avidin. II. Isolation, composition, and amino acid sequences of the tryptic peptides.". Huang T.-S., DeLange R.J.. J ...
LumAvidin Microspheres are avidin-coupled polystyrene microparticles that have been color coded into 100 spectrally distinct ... Avidin coupled, non-magnetic microspheres-the easiest way to get your peptide or protein of interest on a bead. ... LumAvidin® Microspheres are avidin-coupled polystyrene microparticles that have been color coded into 100 spectrally distinct ... sets, or "regions." Similar to an avidin-coated ELISA plate, when reacted with a biotinylated ligand, such as a peptide, each ...
Abcam provides specific protocols for Endogenous Avidin + Biotin Blocking System (ab3387) : Endogenous Avidin + Biotin Blocking ...
Abcam provides specific protocols for Anti-Avidin antibody (HRP) (ab7233) : Immunohistochemistry protocols, Western blot ...
Fluorescein Avidin DCS, Texas Red Avidin D and AMCA Avidin D. ... Avidin Kit contains 500g of three fluorescent avidin products: ... The Fluorescent Avidin Kit contains 500g of three fluorescent avidin products: Fluorescein Avidin DCS, Texas Red Avidin D and ... 500 µg of three fluorescent avidin products: Fluorescein Avidin DCS, Texas Red® Avidin D and AMCA Avidin D. ... Texas Red Avidin D is deep red (excites at 595 nm and emits at 615 nm), and AMCA Avidin D is blue (excites at 350 nm and emits ...
... is a highly fluorescent conjugate of Avidin D and sulforhodamine 101. Texas Red,sup,®,/sup, Avidin D excites at about 595 nm ... Avidin D, Texas Red® labeled, is a highly fluorescent conjugate of Avidin D and sulforhodamine 101. Texas Red® Avidin D excites ... Avidin D, Texas Red® labeled, is a highly fluorescent conjugate of Avidin D and sulforhodamine 101. Since the excitation and ... Avidin D can be employed with Fluorescein Avidin D or other fluorescein conjugates to simultaneously localize two antigens in ...
Our results support the hypothesis that cytoplasmic proteins abundant in fungal fruiting bodies are involved in fungal resistance against predation. The toxicity of these proteins toward stylet-feeding nematodes, which are also capable of feeding on plants, and the abundance of these proteins in edible mushrooms, may open possible avenues for biological crop protection against parasitic nematodes, e.g., by expression of these proteins... ...
... the unbinding of biotin from avidin by means of external harmonic forces with force constants close to those of AFM cantilevers ... Molecular dynamics study of unbinding of the avidin-biotin complex Biophys J. 1997 Apr;72(4):1568-81. doi: 10.1016/S0006-3495( ... We report molecular dynamics simulations that induce, over periods of 40-500 ps, the unbinding of biotin from avidin by means ... the role of key residues contributing to adhesion as well as the spatial range over which avidin binds biotin. In contrast to ...
... avidin explanation free. What is avidin? Meaning of avidin medical term. What does avidin mean? ... Looking for online definition of avidin in the Medical Dictionary? ... These technical questions were examined several years ago with a proof-of-principle product, avidin. Avidin is a protein ... Avidin , definition of avidin by Medical dictionary https://medical-dictionary.thefreedictionary.com/avidin ...
Order this anti-Avidin antibody. , Product number ABIN116961 ... Rabbit Polyclonal Avidin antibody for EIA, IHC (fro), IHC (p), ... anti-Avidin antibody (Biotin) Avidin antibody (Biotin). Details for Product anti-Avidin Antibody No. ABIN116961, Supplier: Log ... Product Details anti-Avidin Antibody References Handling Application Details Target details back to top ... References for anti-Avidin antibody (ABIN116961) Handling Application Details Target details back to top ...
... avidin used in a wide variety of protein detection applications, from Santa Cruz. , Molecular Weight: ~60kD ... Avidin-D is a deglycosylated avidin purified from egg white with a neutral isoelectric point (pI = 6.3). Avidin-D is preferred ... Avidin-D (deglycosylated) Neutralized (pI = 6.3) avidin used in a wide variety of protein detection applications *Home. ... Native Avidin (sc-362068), and/or Streptavidin (sc-363872) bind extremely well with Biotin, but they may exhibit non-specific ...
Thermo Scientific™ DyLight™ streptavidin and avidin conjugates are preparations of biotin binding proteins labeled with Thermo ...
The avidin-biotin interaction is widely employed in the design of functional protein self-assemblies. Herein we assessed how ... supramolecular complex formation by site-specific avidin-. biotin. interactions Yutaro Mori,a Rie Wakabayashi,a Masahiro Gotoab ... The avidin-. biotin. interaction is widely employed in the design of functional protein. self-assemblies. Herein we assessed ... supramolecular complex formation by site-specific avidin-. biotin. interactions Y. Mori, R. Wakabayashi, M. Goto and N. Kamiya ...
In this paper, we report on the immobilization of antibodies onto the surface of an optical fiber through an avidin-biotin ... Enhanced biosensor performance using an avidin-biotin bridge for antibody immobilization Author(s): Upvan Narang; George P. ... for the assays in which the antibody is immobilized via avidin-biotin bridges compared to covalent attachment method. ...
Avidin/Biotin blocking kit, GTX30966, Applications: ICC/IF, IHC, WB; Immunocytochemistry/ Immunofluorescence (ICC/IF), ... Tissues may also bind avidin, biotin, peroxidase biotin, peroxidase streptavidin in avidin biotin based Immunohistochemistry. ... Avidin/Biotin blocking kit. Background. Some tissue sections contain endogenous biotin, biotin binding proteins, lectins or non ... It may be necessary to block the tissue with avidin, followed by biotin. These reagents are added before the primary antibody. ...
Tumor Pretargeting: Role of Avidin/Streptavidin on Monoclonal Antibody Internalization. Patrizia Casalini, Elena Luison, Sylvie ... Tumor Pretargeting: Role of Avidin/Streptavidin on Monoclonal Antibody Internalization. Patrizia Casalini, Elena Luison, Sylvie ... Tumor Pretargeting: Role of Avidin/Streptavidin on Monoclonal Antibody Internalization Message Subject (Your Name) has sent you ... Tumor Pretargeting: Role of Avidin/Streptavidin on Monoclonal Antibody Internalization. Patrizia Casalini, Elena Luison, Sylvie ...
Tumor Cell Targeting with Antibody-Avidin Complexes and Biotinylated Tumor Necrosis Factor α. Monica Moro, Micaela Pelagi, ... Tumor Cell Targeting with Antibody-Avidin Complexes and Biotinylated Tumor Necrosis Factor α ... Tumor Cell Targeting with Antibody-Avidin Complexes and Biotinylated Tumor Necrosis Factor α ... Tumor Cell Targeting with Antibody-Avidin Complexes and Biotinylated Tumor Necrosis Factor α ...
In addition, as (strept)avidin possesses 4 binding sites, it was demonstrated that it is possible to immobilize one binding ... Moreover, the incorporation of biotinylated coordination compounds [Ru(bpy)2(Biot-bpy)]2+ in (strept)avidin displays a ... avidin technology. The metal biotinylatedprecursor ensures the activity of the catalyst, whereas the host protein governs the ... In addition, as (strept)avidin possesses 4 binding sites, it was demonstrated that it is possible to immobilize one binding ...
Our main objective was to create new avidin tools with novel properties for avidin-biotin technology which would have improved ... In addition we obtained valuable basic information about avidin. We modified avidin by the means of protein engineering using a ... Avidin is a basic and rather stable tetrameric glycoprotein found in chicken egg-white. It has an enormous affinity toward ... Avidin engineering : modification of function, oligomerization, stability and structure topology. DSpace/Manakin Repository. ...
1 binding mode of GdL3-4 to avidin with the binding affinity as high as that of the parent avidin-biotin complex. The high ... The avidin-GdL3-4 complexes thus obtained exhibit changes in both r1 and r2 that are pH dependent. The results reveal a new ... The binding affinity with avidin was assessed with a fluorescence displacement assay and with MRI phantom experiments in a 3T ... Synthesis and characterization of pH-sensitive, biotinylated MRI contrast agents and their conjugates with avidin S. M. Vibhute ...
... and 204 μg avidin/ml over 12 d. The LC50 was 136 μg avidin/ml (108-188 95% CL). The combined effects of avidin (136 μg avidin/ ... Avidin is a protein found in chicken egg that sequesters biotin and has shown insecticidal properties against a range of insect ... The addition of avidin at the LC50 concentration significantly reduced consumption by neonates, but it did not affect ... Susannah G. Cooper, David S. Douches, and Edward J. Grafius "Insecticidal Activity of Avidin Combined with Genetically ...
  • Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. (wikipedia.org)
  • In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1800 μg per egg). (wikipedia.org)
  • Functional avidin is found only in raw egg, as the biotin affinity of the protein is destroyed by cooking. (wikipedia.org)
  • The name of the protein was later revised to "avidin" based on its affinity for biotin (avid + biotin). (wikipedia.org)
  • citation needed] Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. (wikipedia.org)
  • Given the strength of the avidin-biotin bond, dissociation of the avidin-biotin complex requires extreme conditions that cause protein denaturation. (wikipedia.org)
  • Extensive chemical modification has little effect on the activity of avidin, making it especially useful for protein purification . (bionity.com)
  • Avidin has a very strong affinity for biotin with a K D ( dissociation constant ) of approximately 10 -15 M -1 [1] , the highest known affinity between any protein and its ligand, and, as such, prevents biotin absorption in the gastrointestinal tract. (bionity.com)
  • Avidin is a glycosylated biotin-binding protein that has a basic isoelectric point and is widely used to detect biotinylated probes. (thermofisher.com)
  • The activity of Avidin-D is approximately 13-14 units per mg of protein. (scbt.com)
  • The avidin- biotin interaction is widely employed in the design of functional protein self-assemblies. (rsc.org)
  • Herein we assessed how the spatial arrangement of the avidin- biotin interaction between protein building blocks affects the formation of a protein supramolecular complex (PSC). (rsc.org)
  • 1. After protein blocking step, soak blot in avidin reagent A, diluted 1:20 with tris/saline buffer for 5-10 minute. (genetex.com)
  • We modified avidin by the means of protein engineering using a rational mutation design strategy. (jyu.fi)
  • Avidin is a protein found in chicken egg that sequesters biotin and has shown insecticidal properties against a range of insect. (bioone.org)
  • While a control protein (Avitag-GFP) is successfuly purified on monomeric avidin column, our protein always goes through the column and does not bind. (protocol-online.org)
  • Avidin, a biotin-binding protein known to be insecticidal, was evaluated as a possible 'carrier' protein. (bl.uk)
  • Recombinant avidin was produced in high yields using Pichia pastoris and was compared to the native egg white protein. (bl.uk)
  • Numerous attempts were made to produce a fully functional insecticidal avidin-based fusion protein containing scorpion or spider toxins by expression as recombinant proteins in P. pastoris but, following tests against Mamestra brassicae (cabbage moth) larvae, the fusion proteins were found to be non-toxic. (bl.uk)
  • The OX26 antibody was affinity purified from ascites fluid and was covalently coupled via a thioether linkage to avidin, and the conjugate was purified to homogeneity by gel filtration fast protein liquid chromatography. (aspetjournals.org)
  • Avidin-dependent redirection of transduction through a variety of biotinylated ligands is greatly dependent on the nature of the biotinylated capsid protein. (rice.edu)
  • In this study, we applied sonodynamic therapy to cancer cells based on the delivery of titanium dioxide (TiO2) nanoparticles (NPs) modified with avidin protein, which preferentially discriminated cancerous cells from healthy cells. (semanticscholar.org)
  • Background and aims: Chicken avidin is a homotetrameric β-barrel protein, whose affinity towards its natural ligand biotin is the strongest non-covalent protein-ligand interaction known. (uta.fi)
  • Residues lining these cavities have been selected as targets for mutagenesis in studies aiming towards the development of novel avidin-based protein structures with different functions. (uta.fi)
  • Hydrogen bonding patterns, ligand motion, the mobilities of individual residues as a function of time and with respect to those of apo-avidin were calculated, and protein-ligand contact area was estimated. (uta.fi)
  • Below are the list of possible Avidin-related protein products. (mybiosource.com)
  • Alternatively, protein immobilization techniques on chemically derivatized beads are well described.10 Herein, we report the detection of the interaction between avidin immobilized onto acrylic resin beads and biotin labelled with a metallocarbonyl tag via an amineterminated polyethylenimine dendrimer. (docme.ru)
  • High-resolution crystal structure of an avidin-related protein: insight into high-affinity biotin binding and protein stability. (semanticscholar.org)
  • Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and specificity consisting of 128 amino acids and has a molecular mass of about 67K Daltons. (neobiolab.com)
  • Binding activity of Avidin-Biotin is 10-15units/mg protein. (neobiolab.com)
  • Avidin prevents biotinylated proteins from binding to Strep-Tactin® or Strep-Tactin XT columns during Twin-Strep-tag and Strep-tag® protein purification. (iba-lifesciences.com)
  • The avidin-biotin system utilizes biotin, a low molecular weight vitamin, which can be chemically coupled to low or high molecular weight molecules such Protein, DNA or hormones. (blogspot.com)
  • The Avidin: Biotin complex takes advantage of two important properties of avidin protein: 1) Very high affinity forBiotin (over one million times higher than antibody for most antigens), and 2) Multiple fourBiotin-binding sites. (glycomatrix.com)
  • However, the basis of the model relies on the strong interaction between biotin and avidin or streptavidin, which are considered to be some of the strongest non-covalent interactions between a protein and ligand. (news-medical.net)
  • The biotin-avidin/streptavidin interactions are some of the strongest known non-covalent interactions and we can harness their power to help us purify proteins by sticking a biotin mimic as a tag on our protein and binding it to little beads coated with a streptavidin mimic. (thebumblingbiochemist.com)
  • It looks (and acts) A LOT like another protein called avidin even though sequence-wise they're only 30% identical. (thebumblingbiochemist.com)
  • Biotin's affinity for the protein avidin makes it ideal for protein and nucleic acid detection or purification methods. (emerging-researchers.org)
  • In this study, Tat protein, which has been proposed as the specific target for AIDS vaccine, was separated and purified from a complex mixture of proteins, known as bacterial lysate (BL) using avidin-biotin interaction in 4-stack microfiltration membranes system. (elsevier.com)
  • The critical factors involved in the process, mainly, the accessibility of the covalently immobilized avidin sites by the biotinylated protein and the associated fouling of the membranes due to the permeation of proteins, were also studied. (elsevier.com)
  • Avidin biotin-binding protein immobilized onto beaded agarose resin for affinity purification methods involving biotinylated proteins and ligands. (fishersci.com)
  • Our avidin, fluorescein conjugate has excitation and emission maxima ~494/518. (thermofisher.com)
  • Avidin D, Texas Red ® labeled, is a highly fluorescent conjugate of Avidin D and sulforhodamine 101. (vectorlabs.com)
  • This Avidin, Peroxidase Conjugate is validated for use in EIA Immunohistochemistry. (emdmillipore.com)
  • A conjugate between avidin and biotinylated leucomyosuppressin (LMS), a myoinhibitory peptide hormone that affects gut contractions in insects, was prepared and fed to aphids. (bl.uk)
  • The avidin : biotin-LMS conjugate had insecticidal activity towards A. pisum when fed in diet at levels which neither of the components (avidin or biotin-LMS) caused significant mortality. (bl.uk)
  • It was hypothesised that binding to the gut, through the avidin moiety, was responsible for the observed oral toxicity of the avidin : biotin-LMS conjugate. (bl.uk)
  • A conjugate between avidin and biotinylated allatostatin was prepared and fed to M. brassicae larvae. (bl.uk)
  • Biotin delivery to brain with a covalent conjugate of avidin and a monoclonal antibody to the transferrin receptor. (aspetjournals.org)
  • The biotin binding capacity of the avidin-OX26 conjugate was measured, and 2.3 biotin binding sites per avidin-OX26 (1:1) conjugate were detected. (aspetjournals.org)
  • Transcytosis through the BBB of [3H]biotin bound to either unconjugated avidin or to the avidin-OX26 conjugate was measured with an internal carotid artery perfusion/capillary depletion technique. (aspetjournals.org)
  • 3H]Biotin bound to the avidin-OX26 conjugate was transported through the BBB at rates that equaled the rate of transcytosis of the unconjugated OX26 antibody. (aspetjournals.org)
  • The clearance from plasma of [3H]biotin bound to the avidin-OX26 conjugate approximated the rate of clearance of the unconjugated OX26 antibody, and not the rate of clearance of [3H]biotin bound to avidin, which was cleared from plasma at much faster rates. (aspetjournals.org)
  • and (c) lipid encapsulated particles activated with a biotin activating agent, whereby the ligand is conjugated to the particles through an avidin-biotin interaction and the resulting conjugate is bound to the molecular epitopes on such surface. (patents.com)
  • whereby said ligand is conjugated to said emulsion through an avidin-biotin interaction and the resulting conjugate is bound to said biological surface to enhance the acoustic reflectivity thereof for ultrasonic imaging. (patents.com)
  • Pharmacokinetics and organ clearance of a 3'-biotinylated, internally [32P]-labeled phosphodiester oligodeoxynucleotide coupled to a neutral avidin/monoclonal antibody conjugate. (aspetjournals.org)
  • Detection of hybridized probes was carried out with 0.07% avidin FITC conjugate (Sigma) in C buffer (0.1 M NaHCO3 /0.15 M NaCl) for 1 h, followed by two rounds of signal amplification using 2.5% anti-avidin biotin conjugate (Sigma) in blocking buffer (1.26% NaHCO3 , 0.018% sodium citrate, 0.0386% Triton X-100 an 1% non-fat dried milk) for 30 min. (bioz.com)
  • Each treatment with anti-avidin biotin conjugate was followed by a treatment with avidin-FITC. (bioz.com)
  • The probe with Biotin was first stained with ExtraAvidin-Cy3 antibody (Sigma Chemical, St. Louis, MO) and the signal was amplified by orderly adding monoclonal Biotin-conjugated anti-avidin antibody (Sigma Chemical) and ExtraAvidin-Cy3 antibody. (bioz.com)
  • Labeled avidin is allowed to bind to biotin-tagged antibodies to amplify antigen-antibody reactions that may be difficult to visualize. (thefreedictionary.com)
  • Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. (antibodies-online.com)
  • In all cases, we observe an improved performance of the biosensor, i.e., lower limit of detection and wide linear dynamic range, for the assays in which the antibody is immobilized via avidin-biotin bridges compared to covalent attachment method. (spie.org)
  • The avidin-biotin-peroxidase complex enzyme-linked immunosorbent assay (ABC-ELISA) and standard ELISA were used for the detection of Echinococcus granulosus antibody in sera of 101 patients operated on for hydatid disease, 40 patients with miscellaneous nonhydatid diseases, and 61 normal subjects. (ajtmh.org)
  • The ABC methodology consists of using an unlabeled primary antibody, a biotinylated secondary antibody, and a complex of avidin-biotin peroxidase. (labce.com)
  • To simplify coupling of therapeutics to the OX26 antibody, the present studies examine the use of the avidin/biotin system to promote coupling of biotin and biotinylated drugs to brain transport vectors. (aspetjournals.org)
  • Small volumes of Avidin, HRP Labeled secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (mybiosource.com)
  • The avidin-biotin-antibody complex was revealed histochemically using a nickel-intensified diaminobenzidine reaction (Sigma). (bioz.com)
  • There are two main methods of antibody biotinylation, the avidin-biotin complex (ABC) method, and the labeled streptavidin-biotin (LSAB) method, both of which are discussed in detail. (news-medical.net)
  • The basic method of antibody biotinylation can be split into two separate techniques relating to whether avidin or streptavidin interactions are utilized. (news-medical.net)
  • A portion of this mixture is then added to the sample, where remaining biotin-binding sites on the avidin bind to the biotinylated antibody that is already attached to the tissue sample. (news-medical.net)
  • In most cases a probe molecule (antibody) is connected to a marker molecule (fluorophore or nanoparticle) through the biotin-avidin bridge. (emerging-researchers.org)
  • For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support. (wikipedia.org)
  • Deglycosylated, neutral forms of chicken avidin are available through Sigma-Aldrich (Extravidin), Thermo Scientific (NeutrAvidin), Invitrogen (NeutrAvidin), and e-Proteins (NeutraLite). (wikipedia.org)
  • Native Avidin (sc-362068), and/or Streptavidin (sc-363872) bind extremely well with Biotin, but they may exhibit non-specific binding of lectins or other carbohydrate binding proteins, cell surface proteins and Fibronectin. (scbt.com)
  • Thermo Scientific™ DyLight™ streptavidin and avidin conjugates are preparations of biotin binding proteins labeled with Thermo Scientific™ DyLight™ fluorescent dyes for use in high-performance fluorescence assays and cellular imaging experiments. (thermofisher.com)
  • Avidin is readily transported to the haemolymph of lepidopteran larvae after feeding, which suggested that it might replace GNA in synthetic insecticidal fusion proteins. (bl.uk)
  • Conversely, beads made of natural or synthetic polymers are widely used as solid phases for immunoassays.9 Beads covered with a large variety of proteins, including avidin, are commercially available. (docme.ru)
  • The Avidin is purified by affinity chromatographic techniques.The purification procedure ensures minimal contamination by other proteins or DNA.The resulting high activity and purity of the product gives very low non-specific binding (NSB). (neobiolab.com)
  • Avidin or streptavidin can be chemically coupled and are fluorescent, radioactive, or other types of macromolecules (proteins,polysaccharides, etc. (blogspot.com)
  • These interactions are also immune to the pressures of pH and temperature extremes, as well as high salt concentrations and denaturants, making the biotin-avidin/streptavidin interaction incredibly useful for targeting proteins of interest. (news-medical.net)
  • After more than five years of research, e-Proteins has implemented a innovative process that enables to produce a Poly-neutralite avidin with a profile presenting a higher level of dimers, trimers and quadrimer comparing the the cross-linking steptavidin profile. (e-proteins.com)
  • With Poly (or Oligomerized) NeutraLite Avidin, e-Proteins goes one step further in increasing the intensity of the signal by some 133%, leading to an overall improvement of min 500% in specificity in comparison to the original avidin . (e-proteins.com)
  • Reversibly bind biotinylated proteins and other ligands with monomers of avidin covalently attached as individual subunits onto beaded resins. (fishersci.com)
  • These two SCAs were converted to the biotinylated systems GdL 3 and GdL 4 and their interaction with avidin was investigated. (rsc.org)
  • A novel system for the attachment of targeting ligands to the Ad capsid, based on the extremely strong avidin-biotin interaction, is described herein. (rice.edu)
  • These findings are critical for the design of multiplexed assays where probe molecules are immobilized to biosensors via the avidin?biotin interaction. (soton.ac.uk)
  • The specific interaction between biotin and avidin was exploited in the affinity purification of solid-phase synthesized peptide libraries. (cnrs-orleans.fr)
  • Advantage of Avidin is its ability of reversible interaction, in many applications reversible interaction is desired. (blogspot.com)
  • Biotin-avidin technology is a widely explored interaction in bioscience. (emerging-researchers.org)
  • The Avidin-biotin complex is the highest affinity interaction in nature, and its utilization to biochemistry integrates all of the former approaches. (wikipedia.org)
  • In pretargeted radioimmunotherapy, 2 main approaches can be distinguished: pretargeting strategies based on the avid interaction between streptavidin (SA) or avidin and biotin, and pretargeting strategies based on the use of bispecific antibodies. (snmjournals.org)
  • Biotin-(strept)avidin complex is widely used in biotechnology because of its extremely high binding constant, but there is no report describing spatiotemporally controlled formation of the complex in live cells. (rcsb.org)
  • Here, based on X-ray crystal structure analysis and calorimetric data, we designed and synthesized photoreleasable biotins, which show greatly reduced affinity for (strept)avidin, but recover native affinity after UV irradiation. (rcsb.org)
  • The present work describes our efforts in the design of artificial metalloenzymes based on biotin-(strept)avidin technology. (rero.ch)
  • In addition, as (strept)avidin possesses 4 binding sites, it was demonstrated that it is possible to immobilize one binding site leaving 3 free sites for the incorporation of the biotinylated precursor. (rero.ch)
  • Moreover, the incorporation of biotinylated coordination compounds [Ru(bpy)2(Biot-bpy)]2+ in (strept)avidin displays a significant decrease of binding affinity (5-7 orders of magnitude) than biotin. (rero.ch)
  • Incorporation of achiral biotinylated rhodium−diphosphine complexes into (strept)avidin yields artificial metalloenzymes for the hydrogenation of N-protected dehydroamino acids. (unibas.ch)
  • Soon after, researchers Bayer and Wilchek developed new methods and reagents to biotinylate antibodies and other biomolecules, allowing the transfer of the avidin-biotin system to a range of biotechnological applications. (wikipedia.org)
  • In this paper, we report on the immobilization of antibodies onto the surface of an optical fiber through an avidin-biotin bridge for the detection of ricin, ovalbumin, and Bacillus globigii (Bg). (spie.org)
  • However, the attachment of biotinylated antibodies to avidin-carrying erythrocytes decreases dramatically. (biochemj.org)
  • Antibodies in the Chromocyte database for EAAT1 / Excitatory amino acid transporter 1 and Streptavidin / Avidin. (chromocyte.com)
  • This is where avidin-biotin complexes connected through reporter enzymes are incubated with biotinylated antibodies. (news-medical.net)
  • These membrane-bounded organelles from the interface peak fractions were immunolabeled with antibodies to both cathepsin L and avidin. (nih.gov)
  • Neutralite avidin is deglycosylated version of egg white avidin that retains its affinity for biotin but has a near-neutral pH which minimizes non-specific interactions. (bio-rad-antibodies.com)
  • 2000) Recombinant NeutraLite avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties. (bio-rad-antibodies.com)
  • Since the excitation and emission maxima are well separated from those of fluorescein, Texas Red ® Avidin D can be employed with Fluorescein Avidin D or other fluorescein conjugates to simultaneously localize two antigens in the same tissue section. (vectorlabs.com)
  • Feeding conjugates of avidin with fluorescently labelled biotin, or a fluorescently labelled, biotinylated peptide, showed that the conjugated compound was also retained in the aphid gut after feeding. (bl.uk)
  • Avidin conjugates of peptides that have little or no oral toxicity to insects, as a result of restricted access to sites of action, could have the potential to form a novel class of insecticidal compounds. (bl.uk)
  • The dissociation constant of the avidin-biotin complex is measured to be KD ≈ 10−15 M, making it one of the strongest known non-covalent bonds. (wikipedia.org)
  • Aware of the strength and specificity of the avidin-biotin complex, researchers began to exploit chicken avidin and streptavidin as probes and affinity matrices in numerous research projects. (wikipedia.org)
  • The non-reversible nature of the avidin-biotin complex can limit avidin's application in affinity chromatography applications where release of the captured ligand is desirable. (wikipedia.org)
  • Biotin is a vitamin that is involved in carboxylation-decarboxylation reactions and forms a highly stable complex with Avidin. (csbsju.edu)
  • The avidin-biotin complex is extremely tight and stable, the complex is stable in 9 M Urea. (csbsju.edu)
  • Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin). (rcsb.org)
  • After adding avidin to the modified S1-ADP-Vi, the complex of actin-tropomyosin and S1-ADP-Vi, of which the biotinylated ADP was associated tightly with avidin , was observed by electron microscopy. (thefreedictionary.com)
  • The fluorometric assay and MRI E-titrations revealed a 3 : 1 binding mode of GdL 3-4 to avidin with the binding affinity as high as that of the parent avidin-biotin complex. (rsc.org)
  • We found that immobilization resulted in both a decrease in the rate of binding and an increase in the rate of dissociation leading to immobilized complexes having equilibrium dissociation constants of 7 ± 3 × 10?12 M, higher than the value measured for the complex between biotin-modified oligonucleotide and nonimmobilized avidin and approximately 4 orders of magnitude larger than values for the wild-type avidin?biotin complex. (soton.ac.uk)
  • Furthermore, we demonstrated that the association of the RGD-avidin-TRAIL complex onto endothelial cells enhanced the tumor cell killing activity. (uu.nl)
  • The Avidin: Biotin complex, once formed, remains stable and can be used for several days after formulation. (glycomatrix.com)
  • Avidin Biotin Peroxidase Complex, supplied by Thermo Fisher, used in various techniques. (bioz.com)
  • If avidin is used, the avidin-biotin complex (ABC) method is appropriate. (news-medical.net)
  • We have developed an immunohistochemical technique using the avidin-biotin-peroxidase complex that is equally effective as direct immunofluorescence in detecting IgA. (qxmd.com)
  • Three-dimensional structures of avidin and the avidin-biotin complex. (ui.ac.id)
  • The avidin- GdL 3-4 complexes thus obtained exhibit changes in both r 1 and r 2 that are pH dependent. (rsc.org)
  • This allows a variety of macromolecular complexes to be formed by mixing Avidin (Solution A) with aBiotinylated enzyme (Solution B) prior to use. (glycomatrix.com)
  • A biotinylated enzyme is then pre-incubated with free avidin, resulting in avidin-biotin-enzyme complexes. (news-medical.net)
  • In most cases, the ABC method will be the first consideration unless the avidin-biotin-enzyme complexes become too large and can no longer pass through the tissue. (news-medical.net)
  • Similar to an avidin-coated ELISA plate, when reacted with a biotinylated ligand, such as a peptide, each of these beads can be used as the substrate on which an assay can be performed. (luminexcorp.com)
  • Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White]. (antibodies-online.com)
  • The binding affinity with avidin was assessed with a fluorescence displacement assay and with MRI phantom experiments in a 3T MRI scanner. (rsc.org)
  • A new 'bead method' for Candida mannan detection was compared with avidin biotin enzyme-linked immunosorbent assay (AB-ELISA) using the serum of mice infected with C. albicans. (eurekamag.com)
  • Quantitation of avidin- and cathepsin L-labeled structures in the late endosome preparation used in the fusion assay showed that many endosomes were heavily loaded with avidin but contained very little cathepsin L (Fig. 3). (nih.gov)
  • However, since a significant proportion of cathepsin L-positive, avidin-negative structures were also observed in the endosomal preparation, late endosomes were incubated in the content mixing assay in the absence of lysosomes at 37°C for 10 min. (nih.gov)
  • Here we developed an avidin-based plate assay to quantify histone debiotinylase activities in nuclear extracts. (elsevier.com)
  • Tissues may also bind avidin, biotin, peroxidase biotin, peroxidase streptavidin in avidin biotin based Immunohistochemistry. (genetex.com)
  • Results: Two candidate binding modes for testosterone binding in avidin-based structures were identified using calculations of ligand and residue motion and hydrogen bonding patterns. (uta.fi)
  • Conclusions: Molecular dynamics simulations were found to be a potential tool for studying ligand binding in avidin-based structures. (uta.fi)
  • Accepted 24 November 2003 Molecular recognition between avidin covalently immobilized at the surface of acrylic resin beads and a transition metallocarbonyl tracer of the biotin ligand was detected using diffuse reflectance infrared Fourier transform spectroscopy. (docme.ru)
  • Ark Therapeutics has developed Scavidin, a version of a naturally occurring molecule called avidin , which recognises the cancer-fighting drug used in radiotherapy and chemotherapy and then "shuttles" it back and forth in the body, picking up the cancer drugs and taking them to the heart of the tumour. (thefreedictionary.com)
  • The avidin : biotin-allatostatin was non-toxic, most likely due to cleavage between the biotin molecule and allatostatin as a result of the high levels of gut proteolysis in lepidoptera. (bl.uk)
  • Avidin-Biotin is a modified form of affinity purified avidin that combines the high affinity specific activity of the native egg white avidin to biotin molecule together with a very low background of the streptavidin produced by the bacteria Streptomyces avidinii. (neobiolab.com)
  • The purification of the peptide mixture containing N-biotinylated sequences was performed by affinity chromatography on an avidin-agarose column. (cnrs-orleans.fr)
  • Biotin Agarose Resin is used for purification or removal of avidin or streptavidin samples. (abtbeads.com)
  • Targeted sonocatalytic cancer cell injury using avidin-conjugated titanium dioxide nanoparticles. (semanticscholar.org)
  • Absolute Mag™ Avidin Magnetic Dextran Nanoparticles, 100 nm (# WHM-G074) are superparamagnetic iron oxide in a matrix of dextran. (cd-bioparticles.com)
  • These nanoparticles are designed with covalently bound avidin on the surface for binding of biotinylated molecules with a high affinity. (cd-bioparticles.com)
  • Switchavidin: reversible biotin-avidin-biotin bridges with high affinity and specificity. (semanticscholar.org)
  • Avidin binds biotin (also known as vitamin H) with an egg-stremely high degree of affinity and specificity making the system a very useful tool in biotechnology and medicine. (ebi.ac.uk)
  • We report molecular dynamics simulations that induce, over periods of 40-500 ps, the unbinding of biotin from avidin by means of external harmonic forces with force constants close to those of AFM cantilevers. (nih.gov)
  • A 68-kD tetrameric glycoprotein found in egg white which has a high affinity for biotin, an association used in immunology for the avidin-biotinylated immunoperoxidase method. (thefreedictionary.com)
  • 10% of the molecular weight is contributed by carbohydrate, composed of four to five mannose and three N-acetylglucosamine residues The carbohydrate moieties of avidin contain at least three unique oligosaccharide structural types that are similar in structure and composition. (wikipedia.org)
  • The Avidin-Biotin cComplex (ABC) method of IHC staining incorporates a large glycoprotein called avidin that has a very high affinity for a low molecular weight vitamin called biotin. (labce.com)
  • In this study, I have used computational methods to model the molecular interactions of two mutant avidins named NRMNH and NQNGY, with testosterone, and those of two keto-biotin binders, T35P,N118M and T35K,N118M, with biotin and keto-biotin. (uta.fi)
  • Avidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containing 4 subunits each with a binding site for biotin. (neobiolab.com)
  • 4. Block with reagent A (Avidin) for 5-10 minutes, rinse with buffer. (genetex.com)
  • The natural function of avidin in eggs is not known, although it has been postulated to be made in the oviduct as a bacterial growth inhibitor, by binding biotin helpful for bacterial growth. (wikipedia.org)
  • The biological function of avidin is not known. (rcsb.org)
  • Chicken egg white avidin conjugated to horseradish peroxidase. (emdmillipore.com)
  • Avidin can be labeled with peroxidase or fluorescein. (labce.com)
  • Eleven immunofluorescence-proved cases of DH were found positive for IgA with the avidin-biotin-peroxidase method. (qxmd.com)
  • All the samples that were positive on direct immunofluorescence were positive with the avidin-biotin-peroxidase method. (qxmd.com)
  • The diagnosis of DH on formalin-fixed tissue is possible with the use of an avidin-biotin-peroxidase method, which is convenient and cost-effective. (qxmd.com)
  • Neutravidin, a deglycosylated avidin with modified arginines, exhibits a more neutral isoelectric point (pI) and is available as an alternative to native avidin, whenever problems of non-specific binding arise. (wikipedia.org)
  • Avidin has a basic isoelectric point (pI) of 10-10.5 and is stable over a wide range of pH and temperature. (bionity.com)
  • Avidin-D is a deglycosylated avidin purified from egg white with a neutral isoelectric point (pI = 6.3). (scbt.com)
  • Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibians. (bionity.com)
  • Avidin is a basic and rather stable tetrameric glycoprotein found in chicken egg-white. (jyu.fi)
  • Avidin-Fluor 550 product is streptavidin-linked fluorophore with 550 absorbance maximum used for fluorescence-based detection of biotinylated molecules. (tymora-analytical.com)
  • The detection and amplification of the hybridization signal was performed using antidigoxigenin-rhodamine for 5S rDNA (Roche) and avidin-FITC and anti-avidin-biotin for 18S rDNA (Sigma). (bioz.com)
  • The simulations reveal a variety of unbinding pathways, the role of key residues contributing to adhesion as well as the spatial range over which avidin binds biotin. (nih.gov)
  • We showed also that the tetrameric quaternary structure of avidin can be broken by only two crucial point mutations in the interface residues and that the resultant monomeric avidin was biologically active in the sense of biotin binding. (jyu.fi)
  • In pretargeting strategies based on biotin and SA or avidin, the use of a clearing agent that could remove the pretargeting construct from the circulation markedly improved the targeting of the radiolabeled biotin to the tumor. (snmjournals.org)
  • Aside from its use in the biotin-avidin system, such SiNPs also are believed to be generally useful fluorescent markers in various kinds of FRET assays, not the least because the fluorophore is located on the surface of the SiNPs (and thus always much closer to the second fluorophore) rather than being doped deep in its interior. (uni-regensburg.de)
  • They both bind biotin - avidin even binds a little stronger - but avidin also has properties that make it less appealing - it's glycosylated, positively-charged, tends to aggregate (clump up) etc. (thebumblingbiochemist.com)
  • The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis. (ui.ac.id)
  • 500 µg of three fluorescent avidin products: Fluorescein Avidin DCS, Texas Red ® Avidin D and AMCA Avidin D. (vectorlabs.com)
  • The surface of the fluorescent SiNPs was biotinylated, and binding of labeled avidin to the surface was studied via FRET in two model cases. (uni-regensburg.de)
  • In the first, FRET occurs from the biotinylated fluorescent SiNP (the donor) to the labeled avidin (the acceptor). (uni-regensburg.de)
  • The addition of avidin at the LC 50 concentration significantly reduced consumption by neonates, but it did not affect consumption by third instars feeding on the susceptible line and the leptine line. (bioone.org)
  • When the avidin concentration in the reaction mixture is less than 15 micrograms/ml, erythrocyte lysis is not induced. (biochemj.org)
  • Researchers have created an avidin with reversible binding characteristics through nitration or iodination of the binding site tyrosine. (wikipedia.org)
  • Avidin monomers can be immobilized and can be used for reversible interactions. (blogspot.com)
  • The binding is very strong, making it suitable for non-reversible binding applications (e.g. removal of avidin components from a sample). (abtbeads.com)
  • We culled the best antiaging products under $20 that are packed with powerful ingredients like Vitamin Outlet Largo Florida Solution Avidin vitamin C (ightens skin evens out hyperpigmentation) retinol (boosts collagen and buffs out dark spots) glycolic acid (exfoliates to boost Alpha Skin Care Intensive Rejuvenating Serum $18. (copernicus-sagres.eu)
  • Avidin is a glycoprotein made of four identical subunits. (csbsju.edu)
  • Specific binding of a biotinylated metallocarbonyl-labelled dendrimer to immobilized avidin detected by diffuse-reflectance infrared Fourier transform spectroscopy. (docme.ru)
  • DOI:10.1002/aoc.591 Chemistry Specific binding of a biotinylated, metallocarbonyllabelled dendrimer to immobilized avidin detected by diffuse-reflectance infrared Fourier transform spectroscopy Bogna Rudolf1 , Janusz Zakrzewski1 *, Grzegorz Celichowski2 , Miche?le Salmain3 , Anne Vessie?res3 ** and Ge?rard Jaouen3 1 University of ?o?dz? (docme.ru)
  • Avidin is a 68,000 M.W. tetrameric biotin-binding glycoprotein with four high affinity binding sites (dissociation constant of 10 -15 M). Conjugated avidin may be used to enhance the sensitivities of immunoassays and immunohistochemical techniques. (emdmillipore.com)
  • In this work, the kinetics and dissociation constant for the binding of a biotin-modified oligonucleotide to microparticle-immobilized avidin were measured. (soton.ac.uk)
  • The measured rate and equilibrium dissociation constants of avidin immobilized by these different methods have been compared with those for nonimmobilized avidin. (soton.ac.uk)
  • Avidin is produced in the oviducts of bird s, reptiles and amphibians and is deposited in the whites of their eggs. (ebi.ac.uk)
  • Recombinant avidin has insecticidal activity towards hemipteran plant pests. (bl.uk)
  • This script performs a pairwise sequence alignment of avidin ( Gallus gallus ) with streptavidin ( Streptomyces lavendulae ). (biotite-python.org)
  • The lack of toxicity was most likely due to incorrect folding of the toxin component of the fusion, since the avidin component was functional. (bl.uk)
  • Functional avidin has four identical cavities that can bind small, hydrophobic molecules. (uta.fi)
  • Avidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. (neobiolab.com)
  • ICAT tagged peptides) can be fractionated using Avidin Chromatography on the AB Vision work station or using a cartridge system. (yale.edu)
  • We succeeded in engineering an avidin with enhanced thermal stability characteristics by introducing cysteine residues to the monomer-monomer interfaces, which formed covalent intermonomeric disulfide bridges. (jyu.fi)
  • Non-covalent binding of avidin to biotinylated erythrocytes results in complement-dependent haemolysis. (biochemj.org)
  • Avidin has been immobilized by both covalent coupling and bioaffinity capture to a surface prefunctionalized with biotin. (soton.ac.uk)
  • Avidin was discovered in egg whites after it was found that animals that were fed an egg-white diet were having health problems. (thebumblingbiochemist.com)
  • They called it "egg white injury" and it turned out that what was happening was that avidin in the egg whites was binding & sequestering biotin (potentially to prevent bacteria from having it). (thebumblingbiochemist.com)