Avian leukosis virus
RNA-Directed DNA Polymerase
Oncogene Proteins v-myb
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Avian Sarcoma Viruses
Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.
Centrifugation, Density Gradient
Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
DNA-Directed DNA Polymerase
DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.
Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed)
Cell Transformation, Viral
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
Moloney murine leukemia virus
Reverse Transcriptase Inhibitors
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Retroviridae Proteins, Oncogenic
A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)
Proto-Oncogene Proteins c-myb
The first of four extra-embryonic membranes to form during EMBRYOGENESIS. In REPTILES and BIRDS, it arises from endoderm and mesoderm to incorporate the EGG YOLK into the DIGESTIVE TRACT for nourishing the embryo. In placental MAMMALS, its nutritional function is vestigial; however, it is the source of INTESTINAL MUCOSA; BLOOD CELLS; and GERM CELLS. It is sometimes called the vitelline sac, which should not be confused with the VITELLINE MEMBRANE of the egg.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Cell Transformation, Neoplastic
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Transformation by v-Myb. (1/401)The v-myb oncogene of the avian myeloblastosis virus (AMV) is unique among known oncogenes in that it causes only acute leukemia in animals and transforms only hematopoietic cells in culture. AMV was discovered in the 1930s as a virus that caused a disease in chickens that is similar to acute myelogenous leukemia in humans (Hall et al., 1941). This avian retrovirus played an important role in the history of cancer research for two reasons. First, AMV was used to demonstrate that all oncogenic viruses did not contain a single cancer-causing principle. In particular, although both Rous sarcoma virus (RSV) and AMV could replicate in cultures of either embryonic fibroblasts or hematopoietic cells, RSV could transform only fibroblasts whereas AMV could transform only hematopoietic cells (Baluda, 1963; Durban and Boettiger, 1981a). Second, chickens infected with AMV develop remarkably high white counts and therefore their peripheral blood contains remarkably large quantities of viral particles (Beard, 1963). For this reason AMV was often used as a prototypic retrovirus in order to study viral assembly and later to produce large amounts of reverse transcriptase for both research and commercial purposes. Following the discovery of the v-src oncogene of RSV and the demonstration that it arose from the normal c-src proto-oncogene, a number of acute leukemia viruses were analysed by similar techniques and found to also contain viral oncogenes of cellular origin (Roussel et al., 1979). In the case of AMV, it was shown that almost the entire retroviral env gene had been replaced by a sequence of cellular origin (initially called mab or amv, but later renamed v-myb) (Duesberg et al., 1980; Souza et al., 1980). Remarkably, sequences contained in this myb oncogene were shared between AMV and the avian E26 leukemia virus, but were not contained in any other acutely transforming retroviruses. In addition, the E26 virus contained a second sequence of cellular origin (ets) that was unique. The E26 leukemia virus was first described in the 1960s and causes an acute erythroblastosis in chickens, more reminiscent of the disease caused by avian erythroblastosis virus (AEV) than by AMV (Ivanov et al., 1962). (+info)
Retrovirus DNA termini bound by integrase communicate in trans for full-site integration in vitro. (2/401)Integration of linear retrovirus DNA involves the concerted insertion of the viral termini (full-site integration) into the host chromosome. We investigated the interactions that occur between long terminal repeat (LTR) termini bound by avian retrovirus integrase (IN) for full-site integration in vitro. Wild-type (wt) or mutant LTR donors that possess gain-of-function ("G") or loss-of-function ("L") for full-site integration activity were used. G LTR termini are characterized as having significantly higher strand transfer activity than the wt and the L LTR termini. L LTR mutations are classified as partially or extremely defective for strand transfer activity. The L mutations were further classified by their ability to either permit or block the assembly of G or wt LTR termini into nucleoprotein complexes capable of full-site strand transfer. We demonstrated that avian myeloblastosis virus IN bound to G LTR termini increased the incorporation of partially defective L LTR termini into nucleoprotein complexes that were capable of full-site integration. The observed full-site integration activity of these assembled nucleoprotein complexes appeared to be influenced by each individual IN-LTR complex in trans. In contrast, extremely defective L LTR termini exhibited the ability to effectively block the assembly of wt LTR termini into nucleoprotein complexes capable of full-site strand transfer. Data from nonspecific DNA competition experiments suggested that IN had an apparent higher affinity for G LTR donor termini than for partially defective L LTR donor termini as measured by full-site integration activity. However, assembled nucleoprotein complexes containing either two G or two L LTR donors were stable, having a similar half-life of approximately 2 h on ice. The results suggest that LTR termini bound by IN exhibit an allosteric effect to modulate full-site integration in vitro. Similar regulatory controls also appear to exist in vivo between the wt U3 and wt U5 LTR termini in retroviruses as well as purified retrovirus preintegration complexes that promoted full-site integration in vitro. (+info)
Retinoid X receptor suppresses transformation by the v-myb oncogene. (3/401)The v-myb oncogene of avian myeloblastosis virus causes acute monoblastic leukemia in vivo and transforms myelomonocytic cells in culture. Retinoids are potent regulators of proliferation and differentiation in various cell types, and they can initiate differentiation in certain types of leukemic cells. However, the BM2 v-myb-transformed chicken monoblastic cell line is resistant to retinoic acid treatment. We found that overexpression of the retinoid X receptor confers sensitivity of BM2 cells to retinoic acid, resulting in induction of growth arrest and terminal differentiation. In contrast, the frequency of apoptosis was not affected by the retinoid X receptor in this cell type. We also demonstrated that suppression of transformation by v-Myb results from the negative effect of retinoid X receptor on v-Myb transactivation function, similar to that previously described for the retinoic acid receptor. The retinoid X receptor-induced inhibition of transactivation by v-Myb seems to be enhanced by a cell type-specific factor(s), which is not required by retinoic acid receptor. (+info)
Metal-induced infidelity during DNA synthesis. (4/401)The effect of several divalent cations on the accuracy of DNA replication in vitro has been examined. Only Be2+ altered the accuracy of DNA synthesis using purified DNA polymerase (DNA nucleotidyltransferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase; EC 220.127.116.11) from avian myeloblastosis virus. The Be2+-induced base substitutions occurred with all templates and with all nucleotides tested. Analysis of the product by equilibrium density centrifugation and processive hydrolysis with snake venom phosphodiesterase suggested that the noncomplementary nucleotides were present in phosphodiester linkage. Nearest neighbor studies indicated that many of the Be2+-induced errors were present as single base substitutions. The enhancement of error frequency could be duplicated by the pretreatment of the enzyme, but not the template, with Be2+. Glycerol gradient centrifugation dissociated the Be2+-DNA polymerase complex and restored the initial error frequency of the polymerase. Thus, the weak binding of a metal cation to a DNA polymerase could alter the accuracy with which that polymerase copied DNA. Beryllium is a known carcinogen. The potential use of this system as a screening technique to detect chemical mutagens and carcinogens is considered. (+info)
Chromatin as a template for RNA synthesis in vitro. (5/401)RNA transcribed in vitro from myeloblast chromatin by exogenously added RNA polymerase B predominantly consists of short chains that remain in hybrid structure with the template; the remainder of the product is free RNA of heterogeneous size. Addition of polyanions during synthesis caused an increase in the size and amount of free RNA with a concomitant decrease in the proportion of small RNA. The large molecular weight RNA is derived from the short RNA chains, which are synthesized de novo during the reaction in vitro. The effect of polyanions on the size and nature of the product may be related to structural changes induced in the template rather than to an inhibition of nuclease activity. (+info)
A new chemical procedure for 32P-labeling of ribonucleic acids at their 5'-ends after isolation. (6/401)A new technique, which utilizes the chemical reaction between [32P]diimidazolidate of orthophosphate and the cetyltrimethylammonium salt of high-molecular-weight RNA in nonaqueous dimethyl formamide, has been developed for the 32P-labeling of RNAs after isolation. The radioactive label of high specific activity is introduced onto a phosphorylated 5'-end of the RNA and renders it suitable for 5'-terminal group analysis. When the labeling reaction was applied to the 70S RNA of avian myeloblastosis virus, a labeled 35S RNA was isolated on sucrose-dimethyl sulfoxide gradients without apparent degradation. (+info)
Avian retrovirus DNA internal attachment site requirements for full-site integration in vitro. (7/401)Concerted integration of retrovirus DNA termini into the host chromosome in vivo requires specific interactions between the cis-acting attachment (att) sites at the viral termini and the viral integrase (IN) in trans. In this study, reconstruction experiments with purified avian myeloblastosis virus (AMV) IN and retrovirus-like donor substrates containing wild-type and mutant termini were performed to map the internal att DNA sequence requirements for concerted integration, here termed full-site integration. The avian retrovirus mutations were modeled after internal att site mutations studied at the in vivo level with human immunodeficiency virus type 1 (HIV-1) and murine leukemia virus (MLV). Systematic overlapping 4-bp deletions starting at nucleotide positions 7, 8, and 9 in the U3 terminus had a decreasing detrimental gradient effect on full-site integration, while more internal 4-bp deletions had little or no effect. This decreasing detrimental gradient effect was measured by the ability of mutant U3 ends to interact with wild-type U3 ends for full-site integration in trans. Modification of the highly conserved C at position 7 on the catalytic strand to either A or T resulted in the same severe decrease in full-site integration as the 4-bp deletion starting at this position. These studies suggest that nucleotide position 7 is crucial for interactions near the active site of IN for integration activity and for communication in trans between ends bound by IN for full-site integration. The ability of AMV IN to interact with internal att sequences to mediate full-site integration in vitro is similar to the internal att site requirements observed with MLV and HIV-1 in vivo and with their preintegration complexes in vitro. (+info)
Modeling the late steps in HIV-1 retroviral integrase-catalyzed DNA integration. (8/401)Model oligodeoxyribonucleotide substrates representing viral DNA integration intermediates with a gap and a two-nucleotide 5' overhang were used to examine late steps in human immunodeficiency virus, type 1 (HIV-1) retroviral integrase (IN)-catalyzed DNA integration in vitro. HIV-1 or avian myeloblastosis virus reverse transcriptase (RT) were capable of quantitatively filling in the gap to create a nicked substrate but did not remove the 5' overhang. HIV-1 IN also failed to remove the 5' overhang with the gapped substrate. However, with a nicked substrate formed by RT, HIV-1 IN removed the overhang and covalently closed the nick in a disintegration-like reaction. The efficiency of this closure reaction was very low. Such closure was not stimulated by the addition of HMG-(I/Y), suggesting that this protein only acts during the early processing and joining reactions. Addition of Flap endonuclease-1, a nuclease known to remove 5' overhangs, abolished the closure reaction catalyzed by IN. A series of base pair inversions, introduced into the HIV-1 U5 long terminal repeat sequence adjacent to and/or including the conserved CA dinucleotide, produced no or only a small decrease in the HIV-1 IN-dependent strand closure reaction. These same mutations caused a significant decrease in the efficiency of concerted DNA integration by a modified donor DNA in vitro, suggesting that recognition of the ends of the long terminal repeat sequence is required only in the early steps of DNA integration. Finally, a combination of HIV-1 RT, Flap endonuclease-1, and DNA ligase is capable of quantitatively forming covalently closed DNA with these model substrates. These results support the hypothesis that cellular enzyme(s) may catalyze the late steps of retroviral DNA integration. (+info)
Identification of the Avian Myeloblastosis Virus Genome | Springer for Research & Development
In addition to neoplasias caused in chickens by helper viruses of the avian myeloblastosis virus (AMV) complex, acute myeloblastic leukemia is induced by a defective leukemogenic component. To...
Avian myeloblastosis ATCC ® VR-1542AS-Gt™
Avian myeloblastosis ATCC ® VR-1542AS-Gt™ Designation: antiserum against AMV RT [NCI HE 599] Application: goat antiserum against the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT)
Dissociation and isolation of the subunits of avian myeloblastosis vir by Mae Joanne Rosok
Rosok, Mae Joanne, Dissociation and isolation of the subunits of avian myeloblastosis virus RNA-directed DNA polymerase (1977). Graduate Student Theses, Dissertations, & Professional Papers. 6828 ...
Activation of v-Myb avian myeloblastosis viral oncogene homolog-like2 (MYBL2)-LIN9 complex contributes to human...
Up-regulation of the v-Myb avian myeloblastosis viral oncogene homolog-like2 B-Myb (MYBL2) gene occurs in human hepatocellular carcinoma (HCC) and is associated with faster progression of rodent hepatocarcinogenesis. We evaluated, in distinct human HCC prognostic subtypes (as defined by patient survival length), activation of MYBL2 and MYBL2-related genes, and relationships of p53 status with MYBL2 activity. Highest total and phosphorylated protein levels of MYBL2, E2F1-DP1, inactivated retinoblastoma protein (pRB), and cyclin B1 occurred in HCC with poorer outcome (HCCP), compared to HCC with better outcome (HCCB). In HCCP, highest LIN9-MYBL2 complex (LINC) and lowest inactive LIN9-p130 complex levels occurred. MYBL2 positively correlated with HCC genomic instability, proliferation, and microvessel density, and negatively with apoptosis. Higher MYBL2/LINC activation in HCC with mutated p53 was in contrast with LINC inactivation in HCC harboring wildtype p53. Small interfering RNA ...
Avian myeoloblastosis virus (AMV): only one side of the coin | Retrovirology | Full Text
The avian myeloblastosis virus (AMV) is an alpha retrovirus responsible for acute myeloblastic leukemia (AML) when injected in ovo, or in newly hatched chickens . Early in vitro dose response experiments indicated that the production of virion with leukemogenic potential required a double infection with AMV and a helper virus . The AMV strains that are used and commercially available, are derived from the orignal BAI strain A purified from chicken leukemic plasma . Leukemic plasma containing the BAI strain has been widely distributed for many years by Life Sciences Inc., in Florida which has been the official provider of national agencies in the USA. The Standard AMV-S BAI strain is a complex mixture of viruses that also includes two helper viruses in addition to AMV. The helper viruses Myeoloblastosis Associated Virus (MAV) contained in AMV-S belong to two different serological subgoups (type1 and type2, also called A and B). Both of them are oncogenic .. Both MAV-1 and MAV-2 ...
Immune Stimulation of Sensitized Chicken Lymphocytes by Avian Retrovirus Proteins | Microbiology Society
Summary Peripheral blood lymphocytes of chickens bearing tumours induced by avian sarcoma virus can be specifically stimulated to divide by the crude culture fluids of virus-infected cells. In this communication, we show that relevant antigenic activity apparently resides in each of the internal virus proteins p15 and p27. The ability of infectious culture fluids to be mitogenic for sensitized lymphocytes is greatly reduced following treatment with antibodies specific for either total avian myeloblastosis virus (AMV) protein or for p27.
Primers 1 and 2 were designed from the mRNA of a guinea pig BK B2 receptor (GenBank accession no. AJ003243) to amplify a 500-base pair fragment. Primers 3 and 4 were a pair of degenerate primers used to amplify a 401-base pair fragment for the guinea pig BK B1 receptor. Primers 3 and 4 were made from the conserved region between mouse BK B1 receptor (GenBank accession no. NM_007539) and rat BK B1 receptor (GenBank accession no. U66107). Amplification was done with an iCycler (Bio-Rad, Hercules, CA). PCR cycles consisted of denaturation for 1 min at 94°C, annealing at 56°C for 90 s, and extension at 72°C for 90 s. A total of 30 cycles was followed by completion of extension for 7 min at 72°C. PCR product (12 μl) was analyzed by electrophoresis on 2% agarose gel. Sequencing was done with an ABI 373XL DNA sequencer after the PCR product was purified with Qiaquick PCR purification kit (QIAGEN, Valencia, CA). RT-PCR without avian myeloblastosis virus reverse transcriptase was used as a negative ...
RT Components: 1U/mL RNase inhibitor, 0.625U/mL reverse transcriptase (avian myeloblastosis virus, Boehringer), 1microM G1 primer, 1.75mM dNTPs ...
v-myb myeloblastosis viral oncogene homolog (avian)-like 2 ELISA Kits | Biocompare.com
Compare v-myb myeloblastosis viral oncogene homolog (avian)-like 2 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
MYBL1 (v-myb myeloblastosis viral oncogene homolog (avian)-like 1)
MYBL1 (v-myb myeloblastosis viral oncogene homolog (avian)-like 1), Authors: Olesya Chayka, Arturo Sala. Published in: Atlas Genet Cytogenet Oncol Haematol.
MYB (myeloblastosis oncogene) - KOMP (Knockout Mouse Project)
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
MYBL2 (myeloblastosis oncogene-like 2) - KOMP (Knockout Mouse Project)
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
035170 - B6.129P2-Myb|tm1Cgn|/TbndJ
These |em|Myb|sup|f/f|/sup||/em| floxed mutant mice possess |em|loxP|/em| sites flanking exon 2 of the myeloblastosis oncogene (|em|Myb|/em|) gene. This strain may be useful for studying the proliferation and differentiation of hematopoietic progenitor cells.
MYB overexpression lysate - LY401654 | acris-antibodies.com
MYB overexpression lysate, 0.1 mg. Transient overexpression lysate of v-myb myeloblastosis viral oncogene homolog (avian) (MYB), transcript variant 2
02 Февраля 2014 - AMV by Uchiha-style
Здравсвуйте пользователи форума amv.at.ua, вчера пользователь под ником Olechkalype просила меня выложить супермодные новинки Компьютерные аксессуары/Графические планшеты в 2014, а так же сотовые бренда Intuos Pro Special Ed., так вот наконецто нашла время для этого, админы прошу не удалять, а перенести в соответствующий раздел, если я ошиблась например Компьютерные аксессуары/Графические планшеты ))) всех обнимаю ...
09 Февраля 2014 - AMV by Uchiha-style
http://18.104.22.168/evko.php?url=/products/blood_pressure_heart/lasix/order/ - http://22.214.171.124/images/order/pictures.php[/URL ...
The effect of negative stain, freeze-fracture, and thin-sectioning procedures on the size of several oncornaviruses has been determined. After negative staining, the average outer dimensions measured over several experiments for the C-type avian viruses, avian myeloblastosis virus (AMV) and Rous sarcoma virus, Prague strain, were about 1370 and 1340 Å, respectively. These values were substantially smaller than the outer dimensions of the C-type murine viruses, Friend leukemia virus (FLV), namely, 1470 Å, and Moloney leukemia virus, namely, 1460 Å. The avian viruses were similar in size to the B-type murine virus, mouse mammary tumor virus, which measured about 1290 Å. All experimental values were obtained after glutaraldehyde (1.5%) fixation and uranyl acetate (2%, pH 4.2) staining. Other negative stains, such as sodium phosphotungstate (1%, pH 6.6), lead to disruption of the outer envelope and a concomitant diminution in size. Freeze-fractured preparations of AMV showed particles with or ...
PMID:17822556 - GONUTS
The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which is responsible for the transformation of myelomonocytic cells by avian myeloblastosis virus (AMV). v-Myb is thought to exert its biological effects by deregulating the expression of specific target genes. We have recently demonstrated that the chicken Gas41 gene, whose promoter co-localizes with an origin of DNA replication, is a bona fide Myb target gene. Because of this finding we have asked whether other Myb-regulated genes are also associated with DNA replication origins. ...
The Plant Pathology Journal
The diversity and polymorphisms in the ToMV population present in one ToMV-positive plant per genotype was further assessed. Total RNAs were isolated from the upper leaf of plants at 21 and 48 d.p.i. The complete ToMV ORF4 sequence was amplified using a two-step reverse-transcriptase polymerase chain reaction (RT-PCR) protocol. The first-strand cDNA was synthesized by reverse transcription of total RNAs using random hexamer primers and the Avian myeloblastosis virus reverse transcriptase (Promega Corp., Madison, WI, USA). A 480-bp fragment (corresponding to positions 5703-6182 of the complete ToMV reference sequence, NC_002692) was amplified using primer pair ToMV-F (5′-ATGTCTTACTCAATCACTTC-3′, sense) and ToMV-R (5′-TTAAGATGCAGGTGCAGAGG-3′, antisense) and the TaKaRa LA Taq polymerase (TaKaRa, Bio Inc., Shiga, Japan). PCR amplification was performed under the following cycling conditions: initial denaturation at 94°C for 5 min; 25 cycles of 94°C for 45 s, 54°C for 30 s, and 72°C for ...
Akt Inhibitor MK2206 in Treating Patients With Progressive, Recurrent, or Metastatic Adenoid Cyst Carcinoma - Full Text View -...
PRIMARY OBJECTIVES:. I. To determine the confirmed response rate of patients with progressive, recurrent/metastatic adenoid cyst carcinoma (ACC) treated with v-akt murine thymoma viral oncogene homolog 1 (Akt) inhibitor MK2206 (MK-2206).. SECONDARY OBJECTIVES:. I. To evaluate the progression-free survival (PFS), overall survival (OS), and safety/tolerability for MK-2206 in these patients.. TERTIARY OBJECTIVES:. I. To explore potential genetic/cytogenetic/histopathologic predictors of clinical outcome (i.e., response, PFS, OS) to MK-2206.. II. To explore the hypothesis that MK-2206-mediated Akt inhibition and downregulation of v-myb avian myeloblastosis viral oncogene homolog (c-myb) protein levels in ACC tumors correlates to clinical outcome (i.e., response, PFS, OS).. OUTLINE:. Patients receive Akt inhibitor MK2206 orally (PO) once weekly for 4 weeks. Courses repeat every 28 days in the absence of disease progression or unacceptable toxicity.. After completion of treatment, patients are ...
AMV BBDO | LBBOnline
Little Black Book, 96 Normal 0 false false false EN-GB JA X-NONE
Eugenics and Depopulation: 2008-03-16
During the late 1960s and early 1970s, cell tumor biology researchers determined that synthetic RNA and feline leukaemia virus (FELV) template added to human type C viruses--those associated with cancers of the lymph nodes increased the rate of DNA production (and subsequent provirus and virus reproduction) as much as thirty times.(1) Such hybrid viruses, these researchers reported, may cause many cancers besides leukaemias and lymphomas, including sarcomas. Other NCI and Litton Bionetics teams reported modifying the fortieth discovered simian virus (SV40) by infusing it with nucleic acids from other species including FELV RNA, avian (i.e., chicken) myeloblastosis virus (AMV) RNA, associated with leukemia and sarcoma development, and mouse sarcoma RNA to: 1) make them carcinogenic, 2) prompt extreme immunosuppression in primates,(2,4,11) and 3) study RNA-dependent DNA polymerase (i.e., reverse transcriptase) and its relationship to human carcinogenesis,(6,11-14) For example, early work in ...
AMV First Strand cDNA Synthesis Kit | NEB
The AMV First Strand cDNA Synthesis Kit combines AMV Enzyme Mix and AMV Reaction Mix. The AMV Enzyme Mix is an optimized blend of AMV Reverse Transcriptase and a Murine RNase Inhibitor. AMV Reverse Transcriptase provides first strand cDNA synthesis reactions with a broader optimal reaction temperature from 37°C to 50°C
Reverse Transcriptases | NEB
Reverse transcriptases can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template.
Bleach Blood AMV to Mushroomhead 1200
My myspace is: myspace****/deathfiend77 This is my very first AMV to Mushroomhead 12 Hundred.. I used Windows movie maker, and this is what I got. I think it came out as best as it possibly could considering that I had 1 episode of Bleach ( a very bad filler), and like 3 episodes of blood. So Enjoy :) Mushroomhead Mushroomhead Mushroomhead Mushroomhead Mushroomhead Mushroomhead Mushroomhead Mushroomhead Mushoomhead Mushroomhead Mushroomhead Mushroomhead Mushroomhead Mushroomhead
Chromosome assignments of four mouse cellular homologs of sarcoma and leukemia virus oncogenes<...
TY - JOUR. T1 - Chromosome assignments of four mouse cellular homologs of sarcoma and leukemia virus oncogenes. AU - Sakaguchi, A. Y.. AU - Lalley, P. A.. AU - Zabel, B. U.. AU - Ellis, R. W.. AU - Scolnick, E. M.. AU - Naylor, S. L.. PY - 1984. Y1 - 1984. N2 - Molecular probes for the oncogenes of Rous sarcoma virus (v-src), avian myeloblastosis virus (v-myb), Kirsten murine sarcoma virus (v-Ki-ras), and Harvey murine sarcoma virus (v-Ha-ras) were hybridized to the DNA from mouse-Chinese hamster somatic cell hybrids. The v-src, v-myb, v-Ki-ras, and v-Ha-ras genes each detected one or a few homologous mouse DNA fragments whose segregation was analyzed in cell hybrids. Mouse cellular homologs c-src, c-Ki-ras, c-Ha-ras, and c-myb segregated concordantly with chromosomes 2, 6, 7, and 10, respectively. Comparison with the known locations of human c-src (chromosome 20) and human c-Ha-ras1 (chromosome 11 short arm) suggests that the human and mouse homologs of these two viral oncogenes reside in ...
Nephroblastomas | definition of nephroblastomas by Medical dictionary
Looking for online definition of nephroblastomas in the Medical Dictionary? nephroblastomas explanation free. What is nephroblastomas? Meaning of nephroblastomas medical term. What does nephroblastomas mean?
Difference between revisions of Griffin:Nested RT-PCR - OpenWetWare
The amplification of RNA requires the conversion of the RNA substrate into DNA. This is achieved through the use of a reverse transcriptase such as AMV RT (avian myeloblastis virus reverse transcriptase) or M-MuLV RT (moloney murine leukemia virus reverse transcriptase). The resulting cDNA can be used as a template for a standard PCR. Nested PCR means that two pairs of PCR primers were used for a single locus. The first pair generates an amplicon within the locus as seen in any PCR experiment. The second pair of primers (nested primers) bind within the first amplicon and produce a second PCR product that will be shorter than the first one. The logic behind this strategy is that if the wrong locus were amplified by mistake, the probability is very low that it would also be amplified a second time by a second pair of primers. ...
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nephroblastoma | Britannica.com
nephroblastoma: Malignant renal (kidney) tumour of early childhood. In 75 percent of the cases, the tumour grows before the age of five; about two-thirds of the instances are apparent by two years...
How to Choose the Right Reverse Transcriptase
Different reverse transcriptases have different characteristics, and some are more well suited to specific applications than others. This article provides guidelines on how to choose between reverse transcriptases, comparing the properties of common RT enzymes such as AMV, MMLV and GoScript.
Expression of Avian Retrovirus Transforming Genes - J Parsons
The product of both the viral and cellular myc genes has been implicated as being a primary mediator of events leading to cellular transformation. In addition,...
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C myb genes Dictionary Definition and More from Dictionary.net
Retrovirus- associated DNA sequences ( v- myb) originally isolated from the avian myeloblastosis and E26 leukemia viruses. The proto- oncogene c- myb codes for a nuclear protein involved in transcriptional regulation and appears to be essential for hematopoietic cell proliferation. The human myb gene is located at 6q22- 23 on the short arm of chromosome 6. This is the point of break in translocations involved in T- cell acute lymphatic leukemia and in some ovarian cancers and melanomas. ( From Ibelgaufts, Dictionary of Cytokines, 1995 ...
NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse...
SVEC4-10 and MEF cells (2 × 106) were mock-infected or infected with WT or Δm145 MCMV (1 and 0.5 PFU/cell, respectively). Upon 12 h of infection, the cells were collected and total RNA was isolated by TriPure reagent (Roche Diagnostics) according to the manufacturers instruction. cDNA was synthetized from 1 μg of total RNA by using the 1st Strand cDNA Synthesis Kit for RT-PCR (Roche Diagnostics, containing: oligo (dT)15 primers (0.04 U A260), 1 mM dNTPs, 5 mM MgCl2, 2.5 U/μl RNase inhibitor, AMV RT buffer (×1), and 1 U/μl AMV reverse transcriptase; final volume of reaction, 20 μl) under the following conditions: 10 min at 25°C, 1 h at 42°C, 10 min at 95°C, and 10 min at 4°C. The cDNA was diluted 1:5 in water and 2 μl was used for each quantitative real-time PCR reaction designed to measure the number of MULT-1 and β-actin cDNA copies in each sample and performed on LightCycler (Roche Diagnostics). The MULT-1 cDNA PCR product (185 bp) was amplified by using exon spanning MULT-F ...
Invitrogen™ Cloned AMV First-Strand cDNA Synthesis Kit 100 reactions Invitrogen™ Cloned AMV First-Strand cDNA Synthesis Kit
Invitrogen™ Cloned AMV First-Strand cDNA Synthesis Kit 100 reactions Invitrogen™ Cloned AMV First-Strand cDNA Synthesis Kit RT-PCR Kits
Clinicopathological study of nephroblastomas in Ibadan.
The present study reviews 31 patients with histologically confirmed nephroblastoma seen over a 5-year period (1985-1989). There was a predominance of female patients with a male: female ratio of 1:1.7, and the average age of the patients was 47 month
nsf13584 NSF Graduate Research Fellowship Program | NSF - National Science Foundation
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ވިލްމުސް ޓިއުމަރު - ވިކިޕީޑިއާ
ގުރުދާގެ ކެންސަރު(އިނގިރޭސި ބަހުން: Nephroblastoma)ގެ ތަފާތު ވައްތަރު ތައް ހުރެއެވެ. އޭގެ ތެރެއިން ފާހަގަ ކުރެވޭ އެއް ވައްތަރަކީ ވިލްމްސް ޓިއުމަރު(އިނގިރޭސި ބަހުން: Wilms Tumour) އެވެ. އަނެއް ވައްތަރަކީ ގުރުދާ ސެލް ކެންސަރު(އިނގިރޭސި ބަހުން: Hypernephroma) ގެ ނަމުން މަޝްހޫރު ވެފައިވާ ވައްތަރެވެ. ވިލްމްސްޓިއުމަރު އަށް ނެފްރޯބްލާސްޓޯމާ އޭ ވެސް ކިޔައެވެ. މިޓިއުމަރު އެންމެ ފުރަތަމަ ހޯދީ ޖަރުމަނުގެ ސާޖަރީ ޑޮކްޓަރެއް ކަމުގައި ޑރ ވިލްމްސް އެވެ. އެހެންކަމުން މިޓިއުމަރަށް މިބުނި ޑޮކްޓަރުގެ ނަމުން ނަންދެވުނެވެ. އާންމު ގޮތެއްގައި ވިލްމްސް ޓިއުމަރު ކުރިމަތިވަނީ ...
Oncornavirus | definition of oncornavirus by Medical dictionary
Looking for online definition of oncornavirus in the Medical Dictionary? oncornavirus explanation free. What is oncornavirus? Meaning of oncornavirus medical term. What does oncornavirus mean?
Diagnostics of nephroblastoma (Wilms tumor): Costs for treatment #204723 in Germany | BookingHealth
Diagnostics of nephroblastoma (Wilms tumor) (costs for program #204723) ✔ Academic Hospital Schwabing ✔ Department of Pediatrics ✔ BookingHealth.com
Diagnostics of nephroblastoma (Wilms tumor): Costs for treatment #206387 in Switzerland | BookingHealth
Diagnostics of nephroblastoma (Wilms tumor) (costs for program #206387) ✔ Hirslanden Multispecialty Clinic Aarau ✔ Department of Oncology ✔ BookingHealth.com
Positive Tested Species Reactivity: Avian-myoblastosis-virus-%28AMV%29, Broad-species, Recommended Applications: Function...
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
Inhibition of the RNase H activity of HIV reverse transcriptase by azidothymidylate<...
TY - JOUR. T1 - Inhibition of the RNase H activity of HIV reverse transcriptase by azidothymidylate. AU - Tan, Cheng Keat. AU - Civil, Rigal. AU - Mohsin Mian, A.. AU - So, Antero G.. AU - Downey, Kathleen M.. PY - 1991/12/1. Y1 - 1991/12/1. N2 - The effects of AZTMP and other nucleoside 5′-monophosphates on the RNA-dependent DNA polymerase and RNase H activities of a recombinant HIV reverse transcriptase have been investigated. Both activities are sensitive to inhibition by millimolar concentrations of AZTMP with MgCl2 as divalent cation activator. Substitution of Mn2+ for Mg2+ markedly potentiates the inhibition of RNase H activity by AZTMP, reducing the IC50 from 5 to 0.05 mM. In contrast, Mn2+ does not alter the sensitivity of the RNA-dependent DNA polymerase activity to inhibition by AZTMP. The inhibition of RNase H activity by AZTMP can be reversed by increasing concentrations of the substrate poly(A)/poly(dT), suggesting that AZTMP may compete with the substrate for binding at the ...
Assessing the renal handling of a dietary protein load in patients managed for nephroblastoma
ENGLISH ABSTRACT: Introduction and purpose The aim of the study was to determine the renal handling of a once-off bolus dietary protein load in patients treated for nephroblastoma. Patients who have been managed for nephroblastoma always have suboptimal amounts of kidney tissue as a result of their medical management which includes nephrectomies, chemotherapy and or radiotherapy. Little data are available indicating the extent of renal impairment expected in such patients as a result of their disease and management. The study was to determine whether the use of regular screening tests such as serum urea, creatinine and urine microalbumin, in conjunction with a dietary protein load could help detect early progressive deterioration of kidney function in nephroblastoma patients. Methodology The study was a quantitative non-randomised intervention study in which patients served as their own control before and after a protein load. Thirty-four participants were included in the study. Each participant ...
Wilms Tumor (Nephroblastoma) - Pipeline Review, H2 2016
Wilms Tumor (Nephroblastoma) - Pipeline Review, H2 2016 Wilms Tumor (Nephroblastoma) - Pipeline Review, H2 2016 Summary Global Markets Directs latest Pharmaceutical and Healthcare disease pipeline guide - Market research report and industry analysis - 10561165
Reverse Transcriptases - MP Biomedicals
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
Blast Cells and Myeloblasts Overview
In medicine, the suffix -blast refers to immature, precursor cells or stem cells. These cells are important in disease detection. Learn more.
LineCraft TR : A Futuristic Aircraft Specializes In High Speed, Mid Altitude Flight - Tuvie
LineCraft TR is a single seater concept aircraft that comprises a collection of Ludicrous Speed engines, generating massive amount of gs. The engine contains eight miniatures and two large speed brakes to control the roll and yaw of the aircraft. LineCraft was designed for racing short to medium distances on the sky or over desert areas. This aircraft is specialized in high speed and mid altitude flight. The giant air brakes are required to control the aircraft because of the compact size and light weight. The ultimate speed of the aircraft has made it a good racer but an experienced pilot is required to fly this extraordinary piece.. ...
Identification and characterization of avian retroviruses in chicken
Identification and characterization of avian retroviruses in chicken embryo-derived yellow fever vaccines: investigation of transmission to vaccine recipients.
nephroblastoma Disease Ontology Browser - DOID:2154
Mutations in human and/or mouse homologs are associated with this disease. Synonyms: adult nephroblastoma; adult renal Wilms tumor; childhood renal Wilms tumor; childhood renal Wilms cancer; renal Wilms tumor; Wilms tumor
Lining Up the Scripts: Reverse Transcriptase Comparison Study
In this article we demonstrate the high quality and robustness of the GoScript™ Reverse Transcriptase and support its use for highly sensitive gene expression analysis.
Positive Tested Species Reactivity: Avian-myoblastosis-virus-%28AMV%29, Toxoplasma-gondii, Recommended Applications: Chromatin...
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
AMV#42 - The Numbers Station - Atoms, Motion & the Void
Part 3 of the 6 part series, The Numbers Station, in which Sherwin finds a curiously familiar cabin in a curiously unfamiliar spot. With lovely art by Kevin Kobasic. Find me on twitter @sherwinsleeves. And on Facebook. ...
"Subcellular localization of proteins encoded by oncogenes of avian myeloblastosis virus and avian leukemia virus E26 and by ... The Myb gene family is named after the eponymous gene in Avian myeloblastosis virus. The viral Myb (v-Myb, P01104) recognizes ... "Entrez Gene: v-myb myeloblastosis viral oncogene homolog (avian)". Klempnauer KH, Symonds G, Evan GI, Bishop JM (June 1984). " ... Myb proto-oncogene protein is a member of the MYB (myeloblastosis) family of transcription factors. The protein contains three ...
William A. Haseltine
"Specific Binding of Tryptophan Transfer RNA to Avian Myeloblastosis Virus Reverse Transcriptase". Proceedings of the National ... They proposed to use methods similar to those used to isolate the HTLV virus to find this new virus. These methods were ... IN: HIV and Other Highly Pathogenic Viruses. pp. 25-32. Haseltine, WA (1988). "Replication and Pathogenesis of the AIDS Virus ... "Gibbon Ape Leukemia Virus Hall's Island: New Strain of Gibbon Ape Leukemia Virus". Journal of Virology. 29 (1): 395-400. doi: ...
Species include the Rous sarcoma virus, avian leukosis virus, and avian myeloblastosis virus. Not all animals that can infect ... The tumor caused by the virus is usually in the form of lymphoma and leukemia. It occurs after a long and latent process. The ...
Antimicrobial properties of copper
Vasudevachari, M; Antony, A (1982). "Inhibition of avian myeloblastosis virus reverse transcriptase and virus inactivation by ... The result is inactivation of bacteria or viruses. Copper complexes form radicals that inactivate viruses. Copper may disrupt ... Influenza A virus was found to survive in large numbers on stainless steel. Once surfaces are contaminated with virus particles ... "Viruses Influenza A". Archived from the original on 2009-10-18. Retrieved 2010-04-07. Barker, J; Vipond, IB; Bloomfield, SF ( ...
This region gets its name from a viral protein called Myb derived from the avian myeloblastosis virus. Specifically, the ...
Maurice Green (virologist)
Grandgenett, D. P.; Gerard, G. F.; Green, M. (1973). "A Single Subunit from Avian Myeloblastosis Virus with Both RNA-Directed ... Later, Green's research extended to the RNA tumor viruses (tumor inducing viruses with an RNA genome). Before reverse ... His studies included characterizing the viruses' DNA, investigating the tumor-inducing properties of the viruses, and ... workers conducted important studies on the biochemical features of reverse transcriptases of avian and murine RNA tumor viruses ...
AMV reverse transcriptase from the avian myeloblastosis virus also has two subunits, a 63 kDa subunit and a 95 kDa subunit. ... Konishi A, Yasukawa K, Inouye K (July 2012). "Improving the thermal stability of avian myeloblastosis virus reverse ... Biology portal Viruses portal cDNA library DNA polymerase msDNA Reverse transcribing virus RNA polymerase Telomerase ... ISBN 978-0-87969-382-4. Bernstein A, Weiss R, Tooze J (1985). "RNA tumor viruses". Molecular Biology of Tumor Viruses (2nd ed ...
The AMV reverse transcriptase from the avian myeloblastosis virus may also be used for RNA templates with strong secondary ... Some viruses also use cDNA to turn their viral RNA into mRNA (viral RNA → cDNA → mRNA). The mRNA is used to make viral proteins ... Here, the host cell membrane becomes attached to the virus' lipid envelope which allows the viral capsid with two copies of ... In cellular life, cDNA is generated by viruses and retrotransposons for integration of RNA into target genomic DNA. In ...
... a part of the structure of the brain Avian myeloblastosis virus is a virus of the family Retroviridae that can infect wild and ... a video consisting of anime clips arranged to a song Science Alfalfa mosaic virus, a plant virus of the family Bromoviridae ...
NASBA (molecular biology)
In order for successful amplification to occur, an enzyme cocktail containing, Avian Myeloblastosis Reverse Transcriptase (AMV- ... zika virus, foot-and-mouth disease virus, severe acute respiratory syndrome (SARS)-associated coronavirus, human bocavirus ( ... Collins, RA; Ko, LS; So, KL; Ellis, T; Lau, LT; Yu, AC (2002). "Detection of highly pathogenic and low pathogenic avian ... The NASBA technique has been used to develop rapid diagnostic tests for several pathogenic viruses with single-stranded RNA ...
জৈৱ প্ৰযুক্তি - অসমীয়া ৱিকিপিডিয়া
Rous Sarcoma virus/ RSV, Mouse Mammary tumour virus/MMTV, Avian Myeloblastosis Virus/AMV আৰু Murine Leukaemia Virus/MuLV) পৰা ...
List of MeSH codes (B04)
... leukosis virus, avian MeSH B04.820.650.070.550 - myeloblastosis virus, avian MeSH B04.820.650.070.775 - sarcoma viruses, avian ... avian MeSH B04.909.574.807.070.500 - leukosis virus, avian MeSH B04.909.574.807.070.550 - myeloblastosis virus, avian MeSH ... avian MeSH B04.909.777.731.070.500 - leukosis virus, avian MeSH B04.909.777.731.070.550 - myeloblastosis virus, avian MeSH ... encephalomyelitis virus, avian MeSH B04.820.565.400.410 - hepatitis a virus MeSH B04.820.565.400.410.500 - hepatitis a virus, ...
Gag polyprotein - Avian myeloblastosis virus
Avian myeloblastosis-associated virus 1/2. Avian myeloblastosis-associated virus type 2. Avian leukosis virus HPRS103. Avian ... Rous sarcoma virus. Avian leukosis and sarcoma virus. Avian myeloblastosis-associated virus type 1. Avian leukosis virus ... Avian endogenous virus EV-1. Avian leukosis virus (ALV). Rous-associated virus type 2. Avian leukemia virus. Avian leukosis ... Avian endogenous virus EV-1. Avian leukosis virus (ALV). Rous-associated virus type 2. Gallus gallus (Chicken). Avian leukosis ...
Antigenic variation of the Avian Myeloblastosis Virus obtained from chick embryo fibroblasts | SpringerLink
Immunelektrophoretisch wird ein verschiedenes Verhalten des Vogel-Myeblastosis-Virus nachgewiesen, je nachdem, ob dieses in ... Chick Embryo Antigenic Variation Avian Myeloblastosis Virus Chick Embryo Fibroblast This work was done while the author was at ... Antigenic variation of the Avian Myeloblastosis Virus obtained from chick embryo fibroblasts. ... Immunelektrophoretisch wird ein verschiedenes Verhalten des Vogel-Myeblastosis-Virus nachgewiesen, je nachdem, ob dieses in ...
Identification of the leukemogenic protein of avian myeloblastosis virus and of its normal cellular homologue | PNAS
Identification of the leukemogenic protein of avian myeloblastosis virus and of its normal cellular homologue. W J Boyle, J S ... The genome of the replication-defective avian myeloblastosis virus (AMV) contains an inserted cellular sequence (amv) that is ... Identification of the leukemogenic protein of avian myeloblastosis virus and of its normal cellular homologue ... Identification of the leukemogenic protein of avian myeloblastosis virus and of its normal cellular homologue ...
Identification of the Avian Myeloblastosis Virus Genome | Springer for Research & Development
In addition to neoplasias caused in chickens by helper viruses of the avian myeloblastosis virus (AMV) complex, acute ... In addition to neoplasias caused in chickens by helper viruses of the avian myeloblastosis virus (AMV) complex, acute ... Souza L.M., Bergmann D.G., Baluda M.A. (1981) Identification of the Avian Myeloblastosis Virus Genome. In: Neth R., Gallo R.C ... Silva RF, Baluda MA (to be published) Avian myeloblastosis virus proteins in leukemic chicken myeloblasts. J VirolGoogle ...
Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase) ...
Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase) ... A complex of tRNATrp and the avian myeloblastosis virus reverse transcriptase has been demonstrated using chromatography on ... Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase). ... The ability of tryptophan tRNA (tRNATrp) to initiate reverse transcription of the 70S RNA of avian RNA tumor viruses suggested ...
Extensive in vitro transcription of rous sarcoma virus RNA by avian myeloblastosis virus DNA polymerase and concurrent...
Extensive in vitro transcription of rous sarcoma virus RNA by avian myeloblastosis virus DNA polymerase and concurrent ... Extensive in vitro transcription of rous sarcoma virus RNA by avian myeloblastosis virus DNA polymerase and concurrent ... Extensive in vitro transcription of rous sarcoma virus RNA by avian myeloblastosis virus DNA polymerase and concurrent ... Extensive in vitro transcription of rous sarcoma virus RNA by avian myeloblastosis virus DNA polymerase and concurrent ...
AMV Reverse Transcriptase, recombinant, GMP Grade from Avian Myeloblastosis Virus, expressed in E. coli
Avian Myeloblastosis Virus Reverse Transcriptase | Fisher Scientific | Bioz
Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: ... Fisher Scientific avian myeloblastosis virus reverse transcriptase. Avian Myeloblastosis Virus Reverse Transcriptase, supplied ... avian myeloblastosis virus-reverse transcriptase. (Fisher Scientific, Pittsburgh, PA).. Article Title: Molecular Mechanism of ... avian myeloblastosis virus reverse transcriptase. . .. The reaction mixture was incubated at 42°C for 50 min, and the cDNA ...
How to Choose the Right Reverse Transcriptase
Avian Myeloblastosis Virus Reverse Transcriptase. Avian myeloblastosis virus (AMV) reverse transcriptase is one of the most ... DeStefano, J.J. et al. (1991) Polymerization and RNase H activities of the reverse transcriptases from avian myeloblastosis, ... Moloney Murine Leukemia Virus Reverse Transcriptase. Moloney murine leukemia virus RT is often used for reverse transcription ... Temin, H.M. and Mizutani, S. (1970) RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature 226, 1211-3. ...
Avian myeloblastosis ATCC ® VR-1542AS-Gt™
... goat antiserum against the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) ... Avian myeloblastosis ATCC ® VR-1542AS-Gt™ Designation: antiserum against AMV RT [NCI HE 599] Application: ... Avian myeloblastosis (ATCC® VR-1542AS-Gt™) Classification: Retrovirus, Avian Type C Retrovirus Group / Product Format: frozen ... goat antiserum against the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT). The antiserum was prepared by ...
Avian-myeloblastosis virus-rna is terminally redundant - implications for mechanism of retrovirus replication
AMV Reverse Transcriptase, recombinant, GMP Grade from Avian Myeloblastosis Virus, expressed in E. coli
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William A. Haseltine - Wikipedia
"Specific Binding of Tryptophan Transfer RNA to Avian Myeloblastosis Virus Reverse Transcriptase". Proceedings of the National ... They proposed to use methods similar to those used to isolate the HTLV virus to find this new virus. These methods were ... IN: HIV and Other Highly Pathogenic Viruses. pp. 25-32. Haseltine, WA (1988). "Replication and Pathogenesis of the AIDS Virus ... "Gibbon Ape Leukemia Virus Halls Island: New Strain of Gibbon Ape Leukemia Virus". Journal of Virology. 29 (1): 395-400. doi: ...
Alpharetrovirus - Wikipedia
Molecular Analysis of Echovirus 13 Isolates and Aseptic Meningitis, Spain - Volume 9, Number 8-August 2003 - Emerging...
1 U of avian myeloblastosis virus RT; and 1 U of Thermus flavus DNA polymerase and Rnase-free distilled water to a final volume ... Virus taxonomy. Seventh report of the International Committee on Taxonomy on Viruses. San Diego: Academic Press; 2000. ... The HEV-B cluster includes coxsackie virus B (CBV), coxsackie virus A9, ENV69, and all echoviruses (EV). ... The viruses could also be identified directly from most of the CSF samples assayed (three of them included in this study; other ...
Transcriptional Control of the Hydrogen Cyanide Biosynthetic Genes hcnABC by the Anaerobic Regulator ANR and the Quorum-Sensing...
Avian myeloblastosis virus reverse transcriptase (30 U; Pharmacia) was used to extend the primer in a reaction mixture ... infection with human immunodeficiency virus, or severe burn wounds (16, 29). The pathogenesis of P. aeruginosa infections is ... in Pseudomonas fluorescens strain P3 improve the induction of systemic resistance in tobacco against tobacco necrosis virus. ...
VP7 and VP4 Genotyping of Human Group A Rotavirus in Buenos Aires, Argentina | Journal of Clinical Microbiology
7 U of avian myeloblastosis virus (M5101; Promega); and 20 U of RNasin RNase inhibitor (N2511; Promega). Oligonucleotide ... Viruses.Human rotavirus strains Wa (G1P), DS-1 (G2P), P (G3P), and VA 70 (G4P), propagated in cultures of MA104 ... Detection of virus and coproantibodies in consecutive samples. (A) Consecutive samples from two patients taken at daily ... These results suggest that detection of rotavirus-specific IgM and IgA in the absence of virus is probably more likely for a ...
Antisera and Monoclonal Antibodies Page 8
goat antiserum against the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT). * Product Sheet ... Avian myeloblastosis (ATCC® VR-1542AS-Gt™) ATCC® Number: VR-1542AS-Gt™ Classification: Retrovirus, Avian Type C Retrovirus ... Influenza A virus (ATCC® VR-1287™) ATCC® Number: VR-1287™ Product Format: freeze-dried ... Lone star virus (ATCC® VR-1230AF™) ATCC® Number: VR-1230AF™ Classification: Bunyaviridae ...
Activity of a Chick Collagen Gene in Heterologous and Homologous Cell-Free Extracts | SpringerLink
Patent US4394443 - Method for cloning genes - Google Patents
... reverse transcriptase from avian myeloblastosis virus, from Life Sciences, Inc., St. Petersburg, FL.; .sup.γ-32 P-ATP, of ... Cucumber mosaic virus coat protein gene. US5624805 *. May 12, 1995. Apr 29, 1997. Celtrix Pharmaceuticals, Inc.. Uses for ... Method for detecting, identifying, and quantitating organisms and viruses. US5641632 *. Jun 2, 1995. Jun 24, 1997. Gen-Probe ... Method of protecting plants by introducing a gene coded for a protein which enhances virus infection of host insects. ...
Patent US5284940 - Preparation for nucleic acid samples - Google Patents
Reverse transcription conditions were studied and optimized for obtaining cDNA from the RNA using avian myeloblastosis virus ( ... Hemin does not inhibit the activity of reverse transcriptase purified from avian myeloblastosis virus. ... Cell fusion can be induced either by virus, such as Epstein-Barr or Sendai virus, or polyethylene glycol. Polyethylene glycol ( ... The low copy situation of virus infection is a good case in point. Since the number of HIV infected T4 cells can be as low as ...
Cardiomyopathy in Transgenic myf5 Mice | Circulation Research
Endoplasmic reticulum stress induces PRNP prion protein gene expression in breast cancer | Breast Cancer Research | Full Text
From Gene to Protein: Information Transfer in Normal and Abnormal Cells - 1st Edition
Avian Myeloblastosis Virus (AMV) Linear Duplex DNA: In Vitro Enzymatic Synthesis and Structural Organization Analysis. Studies ... Noncoding Inserts in Hemoglobin and Simian Virus 40 Genes Are Bounded by Self-Complementary Regions. A Variant of Polyoma Virus ... Role of Protein Synthesis in the Replication of the Killer Virus of Yeast. Effects of the Catalytic Subunit of Protein Kinase ... An Endonuclease-Sensitive Region in Simian Virus 40 Chromatin. Expression of Cloned Viral and Chromosomal Plasmid-Linked DNA in ...
Frontiers | CD25+ B-1a Cells Express Aicda | Immunology
Protein Tyrosine Kinase and Phosphatase Expression Profiling - Cancer Research | Sigma-Aldrich
Role of Endothelin in Deterioration of Heart Failure Due to Cardiomyopathy in Hamsters | Circulation
PolymeraseRetrovirusOncogeneGenomeLeukosis virusSarcomaMurine leukemia virusesViralProteinsProteinAntigenic VariationSequencesCharacterizationInfectionTRNATrpGenePolymerasesSubtypes have been identifiedCDNAHerpesvirusReverse transcriptionAntigen inducedMoloneyOncogenesLeukemogenicGenesColiHepatitis C virInfluenza A virusesSpecies1999MammaryStrainTumorsEndogenousChickensOutbreaksVitroIdentificationTumor cellsTypeSwine influenzaBirdsSubtype
- Extensive in vitro transcription of rous sarcoma virus RNA by avian myeloblastosis virus DNA polymerase and concurrent activation of the associated RNase H. (asm.org)
- Conditions are described that promote the efficient reverse transcription of most of Rous sarcoma virus (RSV) RNA sequences by avian myeloblastosis virus DNA polymerase in vitro. (asm.org)
- Under optimal conditions of reverse transcription, we find now that the RNase H associated with the avian myeloblastosis virus DNA polymerase is active in degrading the RNA moiety of the RNA-DNA hybrids synthesized. (asm.org)
- Structural requirements for binding of bovine tRNATrp with avian myeloblastosis virus DNA polymerase. (uni-trier.de)
- The most common genotype of H3N2 virus isolated to date is a triple reassortant containing hemagglutinin (HA), neuraminidase (NA), and PB1 polymerase genes of human influenza virus origin, matrix (M), nonstructural (NS), and nucleoprotein (NP) genes of classical swine influenza virus origin, and PA and PB2 polymerase genes of avian influenza virus origin ( 8 , 15 , 17 ). (asm.org)
- The term "Retroviruses" refer to a family of viruses which contain RNA and which encode a reverse transcriptase (RNA-dependent DNA polymerase). (google.es)
- The avian myeloblastosis virus (AMV) reverse transcriptase, Taq DNA polymerase and other related reagents were products of Promega, USA. (thefreedictionary.com)
- Leis J., Hurwitz J., Joklik W. К The avian myeloblastosis virus RNA - dependent DNA polymerase. (medkursor.ru)
- Lack of exodeoxyribonuc lease activity and error - correcting function in avian myeloblastosis virus DNA polymerase. (medkursor.ru)
- This invention provides a novel formulation of reverse transcriptase enzymes comprising a reverse transcriptase derived from Avian Myeloblastosis Virus and combined with a reverse transcriptase derived from Moloney Murine Leukaemia Virus, and further provides an improved method of amplifying nucleic acid sequences by reverse transcription or coupled reverse transcription and polymerase chain reaction. (freepatentsonline.com)
- In contrast, pyrophosphate is not detectably mutagenic with avian myeloblastosis virus DNA polymerase or DNA polymerases alpha and beta from animal cells, which lack associated proofreading activities. (nih.gov)
- To define the HA mutations related to these antigenic sites, we cloned and sequenced the HA genes amplified by polymerase chain reaction of parent virus and MAb-selected escape mutants. (scribd.com)
- These various acylated derivatives, as well as the free N -trityl substituted DL-phenylalanyl amino acids, were tested for their effect on the DNA polymerase activity of the Moloney murine leukaemia virus reverse transcriptase (M-MuLV RT). (scielo.org.za)
- As HIV uses reverse transcriptase to copy its genetic material and generate new viruses (part of a retrovirus proliferation circle), specific drugs have been designed to disrupt the process and thereby suppress its growth. (bionity.com)
- Identification of nucleotide sequences which may encode the oncogenic capacity of avian retrovirus MC29. (asm.org)
- 2. The method of claim 1 wherein said human retrovirus is a human immunodeficiency virus. (google.es)
- 3. The method according to claim 1, wherein said human retrovirus is selected from the group consisting of human T-cell lymphotropic virus-III, lymphoadenopathy associated virus, and AIDS-related virus. (google.es)
- 4. The method according to claim 1, wherein said human retrovirus is selected from the group consisting of human T-cell lymphotropic virus-I, human T-cell lymphotropic virus-II, and Kawasaki disease causative retroviruses. (google.es)
- 13,20) There is often an underlying viral cause, as with Marek's disease (herpesvirus), myeloblastosis (unknown retrovirus), and lymphoid leukosis (reticuloendotheliosis virus and avian leukosis virus). (thefreedictionary.com)
- Moloney murine leukaemia virus (M-MuLV) is a member of the retrovirus family. (scielo.org.za)
- The genome of the replication-defective avian myeloblastosis virus (AMV) contains an inserted cellular sequence (amv) that is part of the oncogene responsible for acute myeloblastic leukemia in chickens infected with AMV. (pnas.org)
- [ 27 ] The proto-oncogene C-Myb (v-myb myeloblastosis viral oncogene homolog [avian], also known as Myb ) is a critical target of miR-150. (medscape.com)
- Innen dette spesialfeltet har vi levert en rekke bidrag gjennom årene (i tidsskrift som Science, EMBO J, Blood, Oncogene, Nucleic Acids Res, J Biol Chem), godt hjulpet av 11 ferdige Dr.grads-kandidater og 30 Masterstudenter, så vel som av samarbeidende forskere fra syv land. (uio.no)
- As discussed in the introduction and in our recent review, (4) the Abbott Molecular 4-probe melanoma FISH test targeting ras responsive element binding protein 1 (RREB1, 6p25), myeloblastosis viral oncogene homologue (MYB, 6q23), centromere 6, and cyclin D1 (CCND1,11q13) probes5 is the first commercially available FISH probe set showing promise as a diagnostic adjunct in evaluation of melanocytic lesions. (thefreedictionary.com)
- MYB, v-myb myeloblastosis virus oncogene homologue;RREB1, ras responsive element binding protein 1. (thefreedictionary.com)
- The v-myb oncogene causes myeloblastosis in chickens. (bloodjournal.org)
- Souza LM, Baluda MA (to be published) Identification of the avian myeloblastosis virus genome. (springer.com)
- Souza LM, Komaromy MC, Baluda MA (1980) Identification of a proviral genome associated with avian myeloblastic leukemia. (springer.com)
- Souza LM, Strommer JN, Hillyard RL, Komaromy MC, Baluda MA (to be published b) Cellular sequences are present in the presumptive avian myeloblastosis virus genome. (springer.com)
- Souza L.M., Bergmann D.G., Baluda M.A. (1981) Identification of the Avian Myeloblastosis Virus Genome. (springer.com)
- Part of the neutralizing antigenic site III has been described in this genome region in poliovirus and swine vesicular disease virus. (cdc.gov)
- Analysis of this fragment of the enteroviral genome has permitted the study of important pathogenic structures, such as part of the antigenic sites described for polioviruses ( 13 ), CBV, and swine vesicular disease virus (SVDV) ( 14 , 15 ), and part of the canyon structure, related to viral attachment, which has been described in several HEVs and rhinoviruses ( 16 ). (cdc.gov)
- Reverse-transcribing RNA viruses , such as retroviruses , use the enzyme to reverse-transcribe their RNA genomes into DNA, which is then integrated into the host genome and replicated along with it. (bionity.com)
- Transcription of the viral genome in cell lines transformed by simian virus 40. (uni-trier.de)
- Recombinants between herpes simplex virus types 1 and 2: analyses of genome structures and expression of immediate early polypeptides. (asm.org)
- Absence of circularly permuted and largely redundant sequences in the genome of visna virus. (asm.org)
- Billeter M. A., Coffin J. M., Joho R. Mapping of the genome of prague rous sarcoma virus В. (medkursor.ru)
- This work reports a method for rapid amplification of the complete genome of equine influenza virus subtype 2 (H3N8). (scielo.org.ar)
- The genome of equine influenza A virus contains eight linear segments of negative stranded RNA (about 13.6 kb). (scielo.org.ar)
- Species include the Rous sarcoma virus, avian leukosis virus, and avian myeloblastosis virus. (wikipedia.org)
- Binding of tryptophanyl-tRNA to the reverse transcriptase of replication-defective avian sarcoma viruses. (asm.org)
- Characterization of Rous sarcoma virus src gene products synthesized in vitro. (asm.org)
- Lee S. G., Lipmann F. Isolation from normal and Rous sarcoma virus - transformed chicken fibroblasts of a factor that binds glucose and stimulated its transport. (medkursor.ru)
- Linial M. Infection of resistant avian cells by subroup В Rous sarcoma virus. (medkursor.ru)
- Quntaka R. V., Bishop J. M., Varmus H. E. Covalently closed circular DNA of avian sarcoma virus: purification from nuclei of infected quail tumor cells and measurement by electron microscopy and gel electrophoresis. (medkursor.ru)
- Bandyopadhyay A. K. Partial purification and characterization of DNA polymerases from a Rous sarcoma virus - transformed rat cell line. (medkursor.ru)
- Bergholz С M., Wolfe L. G., Delnhardt F. Oncogenicity in marmosets of HL - 23/V, a type С oncornavirus isolated from human leukemic cells, and comparison with simian sarcoma virus type I (SSV - I/SSAV - I). - «J. nat. (medkursor.ru)
- Brugge J. S., Erikon R. L. Identification of a transformationspecific antigen induced by an avian sarcoma virus. (medkursor.ru)
- 19) which have greatly enhanced opportunities for the study of such difficult systems as the Rous sarcoma virus (20). (chestofbooks.com)
- Like the oncogenic human γ-herpesviruses Kaposi's sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV), MDV-1 encodes several viral microRNAs (miRNAs). (asm.org)
Murine leukemia viruses1
- Replication-defective ecotropic murine leukemia viruses: Detection and quantitation of infectivity using helper-dependent XC plaque formation. (asm.org)
- Linear viral DNA isolated from the cytoplasm of helper virus (MAV-1 or MAV-2) infected chicken embryonic fibroblasts (CEF) has a mass of 5.3 million daltons (md) (Bergmann et al. (springer.com)
- 1980). A Hind III digest of AMV-S linear viral DNA also indicates that more than 90% of the AMV-S virus complex is MAV-1 like, while the remainder represents both the leukemogenic and MAV-2-like viruses (Souza et al. (springer.com)
- Reassortment between human H1N1 and H3N2 viruses is hypothesized to have initially created an H1N2 subtype virus, which subsequently acquired all six of its internal viral protein genes through reassortment with a wholly avian virus ( 2 ). (asm.org)
- Translation of type C viral RNAs in Xenopus laevis oocytes: evidence that the 120,000-molecular-weight polyprotein expressed in Abelson leukemia virus-transformed cells is virus coded. (asm.org)
- In comparison with other viral vectors designed for targeted transgene delivery, this M-PMV VLP system represents the lowest risk of generating virus-associated pathology, as the VLPs do not contain any viral coding sequences and are formed in a cell-free system. (deepdyve.com)
- The reverse transcriptase, which is capable of using the viral RNA as a template for the synthesis of complementary DNA, is essential to the first stage of the virus' self-replication. (google.es)
- Here, we present an analysis of the mRNAs targeted by viral miRNAs expressed in the chicken T-cell line MSB1, which is naturally coinfected with MDV-1 and the related nonpathogenic virus MDV-2. (asm.org)
- RNAseH assays were performed under a wide variety of conditions employing substrate RNAs of Duck Hepatitis B Virus sequence annealed to complementary DNA oligonucleotides and permeabilized intracellular viral core particles. (biomedcentral.com)
- Silva RF, Baluda MA (to be published) Avian myeloblastosis virus proteins in leukemic chicken myeloblasts. (springer.com)
- VP7 and VP4 proteins elicit, independently, neutralizing antibodies and specify the virus G (outer shell glycoprotein) and P (for protease-susceptible protein) serotypes, respectively. (asm.org)
- Most recently it has been shown that H1N2 viruses with HA proteins antigenically related to those of the H1N2 viruses from the United Kingdom are circulating among pigs in Belgium ( 14 ). (asm.org)
- Ruml, Tomáš 2014-04-01 00:00:00 Retroviral gag proteins, as well as fragments minimally containing the capsid (CA) and nucleocapsid (NC) subunits of Gag, are able to spontaneously assemble into virus-like particles (VLPs). (deepdyve.com)
- Because the encoded zebrafish UDP glucuronosyltransferase (UGT) proteins do not display orthologous relationships to any of the mammalian and avian UGT enzymes based on molecular phylogeny, it is difficult to predict their substrate specificity. (aspetjournals.org)
- Naso R. E., Brown R. L. Synthesis and cleavage of Rauscher leukemia virus precursor proteins in synchronized cells. (medkursor.ru)
- Influenza viruses belong to the Orthomyxoviridae family and are classified into three great types: A, B, and C, according to antigenic differences in their nucleoprotein (NP) and matrix (M) proteins. (scielo.org.ar)
- Three antisera raised against distinct synthetic peptides predicted from the long open reading frame of amv specifically precipitated the same 48-kilodalton protein (p48amv) from leukemic myeloblasts but not from normal hematopoietic tissue, fibroblasts, or from fibroblasts infected with the AMV helper virus, MAV-1 (myeloblastosis-associated virus type 1). (pnas.org)
- Avian myeloblastosis virus (AMV) encodes a v-Myb protein that when compared to. (springer.com)
- Cumulatively, the present results provide several lines of evidence that HSV gH/gL interacts with a cell surface cognate protein(s), that this protein is not necessarily an αVβ3 integrin, and that this interaction is required for the process of virus entry/fusion. (pubmedcentralcanada.ca)
- Leamnson R. N., Shander M. H. M., Halpern M. S. A structural protein complex in moloney leukemia virus. (medkursor.ru)
- Luka J., Siegert W., Klein G. Solubilization of the Epstein - Barr virus - determined nuclear antigen and its characterization as a DNA - binding protein. (medkursor.ru)
- Burnette W. N., Holladay L. A., Mitchell W. M. Physical and chemical properties of moloney murine leukemia virus p30 protein: a major core structural component exhibiting high helicity and self - association. (medkursor.ru)
- A screen for protein tyrosine phosphatases (PTPs) expressed in the chick inner ear yielded a high proportion of clones encoding an avian ortholog of protein tyrosine phosphatase receptor Q (Ptprq), a receptor-like PTP. (jneurosci.org)
- Purified plasma membranes from normal rat liver, a rat hepatoma and a rat hepatic fibrosarcoma have been shown to contain a protein which drastically inhibits avian myeloblastosis virus reverse transcriptase activity. (ias.ac.in)
- iii) RNAseH activity has been detected for the human hepatitis B virus when the RNAseH domain was expressed as a fusion with the maltose binding protein [ 24 ]. (biomedcentral.com)
- therefore, the viruses may be subjected to little immune pressure and consequently undergo less antigenic variation than do human strains. (scribd.com)
- To evaluate the level of antigenic variation of these viruses during their maintenance, we previously prepared a panel of monoclonal antibodies (MAbs) to the hemagglutinin (HA) of a recent HlNi swine virus (36). (scribd.com)
- Short helper-virus sequences provide its carboxyl terminus and also may provide the amino terminus. (pnas.org)
- We describe the molecular epidemiology of the EV13 isolates obtained in Spain from the 2000 outbreak by analyzing the VP1 partial sequences of 64 identified viruses. (cdc.gov)
- Benveniste R. E., Callahan R., Todaro G. J. Two direct endogenous type С viruses isolated from the asian rodent Mus cervicolor: conversion of virogene sequences in related rodent species. (medkursor.ru)
- Bergmann DG, Souza LM, Baluda MA (1980) Characterization of the avian myeloblastosis associated virus DNA intermediates. (springer.com)
- The product obtained by this method can be cloned, used in later sequencing reactions or nested-PCR with the purpose of achieving a rapid diagnosis and characterization of the equine influenza virus type A. This detection assay might be a valuable tool for diagnosis and screening of field samples as well as for conducting molecular studies. (scielo.org.ar)
- Chikungunya Virus Acute-phase blood samples were collected from 69 patients with clinically suspected cases of DENV/CHIKV and Dengue Virus co-infection. (cdc.gov)
- Of the 17 CHIKV-positive patients, 10 were male and infection with 2 dengue viruses (DENV-1 and DENV-4) 6 were female. (cdc.gov)
- Pseudomonas aeruginosa is a gram-negative bacterium that can cause serious infections in patients suffering from cystic fibrosis, cancer, infection with human immunodeficiency virus, or severe burn wounds ( 16 , 29 ). (asm.org)
- The emergence of these viruses was remarkable because influenza among American pigs had historically been due almost exclusively to infection with classical H1N1 swine influenza viruses ( 3 , 5 , 11 ). (asm.org)
- Conditions leading to the establishment of the N (a gene dependent) and A (a gene independent) transformed states after polyoma virus infection of rat fibroblasts. (asm.org)
- Does simian virus 40 DNA integrate into cellular DNA during productive infection? (asm.org)
- Electron microscopic study of measles virus infection: cell fusion and hemadsorption. (asm.org)
- There is serious concern that recombination with human influenza viruses may already have occurred raising the danger of human to human infection. (thefreedictionary.com)
- Before the introduction of screening of blood donors for hepatitis C virus (HCV), the risk of acquiring HCV infection as a result of transfusion was about 10% . (who.int)
- Biegeleisen K., Rush M. G. Association of herpes simples virus type I DNA with host chromosomal DNA during productive infection. (medkursor.ru)
- Of particular importance for the present report has been the observation, by electron microscopy (9), of definitive cell ultrastructures specific and, under some conditions, quantitatively related to infection of the cell and liberation of the virus. (chestofbooks.com)
- Dr. Beard: It would seem appropriate to comment on the influence of the host in the occurrence of the malignant process associated with virus infection. (chestofbooks.com)
- The disabling disorder known as chronic fatigue syndrome or myalgic encephalomyelitis (CFS/ME) has been linked in two independent studies to infection with xenotropic murine leukemia virus-related virus (XMRV) and polytropic murine leukemia virus (pMLV). (asm.org)
- A complex of tRNATrp and the avian myeloblastosis virus reverse transcriptase has been demonstrated using chromatography on Sephadex G-100 columns. (caltech.edu)
- C gene (294 bp) of CHIKV were determined by using an hikungunya virus (CHIKV) was isolated in Tanganyi- automated 310 DNA sequencer (Applied Biosystems Inc., ka (now Tanzania) in 1953 ( 1 ). (cdc.gov)
- Phylogenetic analyses demonstrated that Sw/IN/99 had resulted from reassortment between a classical H1N1 swine influenza virus, which supplied its HA gene segment, and one of the triple-reassortant swine H3N2 viruses, which supplied all of the remaining RNA segments ( 7 ). (asm.org)
- Lai Ching-Juh, Nathans D. The ВС gene of simian virus 40. (medkursor.ru)
- Barbacid M., Stephenson J. R., Aaronson S. A. Gag Gene of mammalian type - С RNA tumour viruses. (medkursor.ru)
- Benjamin T. L. Tumor viruses and cellular gene expression. (medkursor.ru)
- We examined the cellular function of the CaMNR1 gene by virus-induced gene silencing and ectopic overexpression in pepper and Arabidopsis plants, respectively. (redorbit.com)
- Avian Myeloblastosis Virus (AMV) Reverse Transcriptase ( NEB #M0277 ) and Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase ( NEB #M0253 ) are RNA-directed DNA polymerases. (neb.com)
Subtypes have been identified1
- At present, 16 HA and 9 NA subtypes have been identified among influenza A viruses. (scielo.org.ar)
- Ten micrograms of RNA treated with DNase I (Ambion, Austin, Tex.) was used for cDNA synthesis with avian myeloblastosis virus reverse transcriptase (Fisher Scientific, Pittsburgh, Pa. (bioz.com)
- The radiolabeled primer was annealed to 10 μg of chlamydial RNA, and cDNA was synthesized with avian myeloblastosis virus reverse transcriptase . (bioz.com)
- Marek's disease virus 1 (MDV-1), an oncogenic α-herpesvirus that induces T-cell lymphomas in chickens, serves as model system to study transformation by lymphotropic herpesviruses. (asm.org)
- Here, we present an analysis of the mRNAs targeted by miRNAs encoded by the oncogenic avian herpesvirus MDV-1 in transformed chicken T cells, including a short list of mRNAs that are also targeted by miR-155 seed family miRNAs in EBV- or KSHV-transformed human B cells, and present an initial functional analysis of some of these miRNA targets. (asm.org)
- 13. A kit for use in a reverse transcription reaction and comprising a reverse transcriptase derived from Moloney Murine Leukaemia Virus and a reverse transcriptase derived from Avian Myeloblastosis Virus together with instructions to combine the reverse transcriptases to form a formulation as claimed in 1 . (freepatentsonline.com)
- Padgett B. L., Hunt J. M., Walker D. L. Specificity of the tumor - specific transplantation antigen induced by JC virus, a human polyomavirus. (medkursor.ru)
- McCater J. A. Genetic studies of the ploidy of moloney murine leukemia virus, - «J. Virol. (medkursor.ru)
- Avian Myeloblastosis Virus (AMV) Reverse Transcriptase and Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase are RTs that are commonly used in molecular biology workflows. (neb.com)
- 1. A formulation having reverse transcriptase activity and comprising a combination of a reverse transcriptase derived from Moloney Murine Leukaemia Virus and a reverse transcriptase derived from Avian Myeloblastosis Virus. (freepatentsonline.com)
- 9. A formulation as claimed in claim 1 wherein the ratio of units of enzyme of Moloney Murine Leukaemia Virus to Myeloblastosis Virus is balanced toward Moloney Murine Leukaemia Virus, preferably being between 3:2 and 7:1 and preferably about 4:1. (freepatentsonline.com)
- A ThermoScript TM reverse transcriptase instead of the avian myeloblastosis virus reverse transcriptase or Moloney murine leukemia virus reverse transcriptase was used. (scielo.org.ar)
- Se utilizó la enzima transcriptasa reversa ThermoScript TM en lugar de la transcriptasa reversa del virus de la mieloblastosis aviar o la transcriptasa reversa del virus de la leucemia murina de Moloney. (scielo.org.ar)
- An increasing array of 5′ nuclease assays have been applied to detect or distinguish between a wide variety of targets including c- erb-2 oncogenes ( 18 ), Vβ repertoire ( 30 ), Salmonella ( 12 ), hepatitis C virus ( 37 , 39 ), human papillomavirus ( 49 ), leafroll virus ( 45 ), and Escherichia coli SLT-1 ( 55 ). (asm.org)
- In addition to neoplasias caused in chickens by helper viruses of the avian myeloblastosis virus (AMV) complex, acute myeloblastic leukemia is induced by a defective leukemogenic component. (springer.com)
- Thus, the avian nephroblastoma is a valid source of cancer-associated genes. (aacrjournals.org)
- Acquisition of oncogenicity by endogenous mouse type C viruses: effects of variations in env and gag genes. (asm.org)
- Our next step, as described in this report, was to identify the genetic changes associated with the four antigenic sites on the HA of HlNi swine viruses by sequencing the HA genes of MAb-selected mutants and the parent strain, Sw/ IN/1726/88 (Sw/IN/88), for comparison with the Hi HA of AIPR/8/34 (6, 21, 32, 42). (scribd.com)
- Lebowitz P., Stem R., Ghosh P. K. Specificity of initiation of transcription of simian virus 40 DNA 1 by Escherichia coli RNA polvmerase: identification and localization of five sites for initiation with (Y - 32P) ATP. (medkursor.ru)
- duck hepatitis B virus RNAseH activity was not detected under any of these conditions, although E. coli or Avian Myeloblastosis Virus RNAseH activity could be detected under all conditions. (biomedcentral.com)
Hepatitis C vir3
- ABSTRACT The seroprevalence of hepatitis C virus (HCV) specific antibodies and HCV genotypes distribution were studied among 559 Iraqi children with thalassaemia in receipt of repeated blood transfusions. (who.int)
- So the aims of this study were to identify the seroprevalence of hepatitis C virus specific antibodies among children with thalassaemia in receipt of blood transfusions in a teaching hospital in Baghdad and to investigate the HCV genotype distribution among these patients. (who.int)
- A new virus, Hepatitis C virus (HCV), which has proven to be the major etiologic agent of blood-borne NANBH, was discovered by Applicant. (freepatentsonline.com)
Influenza A viruses3
- H3N2 influenza A viruses have caused widespread outbreaks of respiratory disease and, in some cases, abortion among pigs throughout the major swine-producing regions of the United States since 1998. (asm.org)
- Influenza A viruses are widespread in nature and cause disease in a variety of species, including humans, lower mammals, and birds (14). (scribd.com)
- Influenza A viruses of the HlNl subtype were first detected in pigs in the United States in 1930. (scribd.com)
- Historically, close contact of fowl with humans has been shown epidemiologically to foster cross-species jump (avian to human) of new influenza strains (e.g. (thefreedictionary.com)
- H5N2 Low Pathogenicity Avian influenza (LPAI) was recorded in ornamental birds and decoy ducks in two provinces in northern Italy in January-February (two regions) and H7N2 LPAI was recorded on a mixed species agritourism farm in a third province in March. (thepoultrysite.com)
- Beth E., Cikes M., Schloen L. Interspecies species- and type - specific determinants of human papovaviruses (JC and BK) and of simian virus 40. (medkursor.ru)
- On the other hand influenza viruses type A have been isolated from many species including humans, pigs, horses, marine mammals and a wide range of domestic and wild birds (10) . (scielo.org.ar)
- All these subtypes are found in avian species. (scielo.org.ar)
- An H1N2 influenza A virus was isolated from a pig in the United States for the first time in 1999 (A. I. Karasin, G. A. Anderson, and C. W. Olsen, J. Clin. (asm.org)
- Phylogenetic analyses of eight such viruses isolated from pigs in Indiana, Illinois, Minnesota, Ohio, Iowa, and North Carolina during 2000 to 2001 showed that these viruses are all of the same reassortant genotype as that of the initial H1N2 isolate from 1999. (asm.org)
- Subsequent to the appearance of these H3N2 viruses, an H1N2 virus (A/Swine/Indiana/9K035/99 [Sw/IN/99]) was isolated from a pig in Indiana during a 6-week-long outbreak of influenza-like illness and abortions on the farm of origin in November 1999. (asm.org)
- Modulation of mouse mammary tumor virus production in the MJY-alpha cell line. (asm.org)
- More recently members of the same group ( 13 ) studying mice with constitutive activation of human Smo under control of the mouse mammary tumor virus (MMTV) promoter, found that mammary ductal cells showed increased proliferation, altered differentiation, and developed ductal dysplasias. (aacrjournals.org)
- Mammary glands from MMTV (mouse mammary tumor virus)- neu/p27 +/− mice exhibited alveolar hyperplasia, enhanced proliferation, decreased apoptosis, and accelerated tumor formation compared to MMTV - neu/p27 +/+ glands. (asm.org)
- The H3N8 subtype of equine influenza virus was first isolated in 1963 during an outbreak of this disease in Miami (prototype strain: A/equine2/ Miami/63) and can be found in different parts of the world, except in Australia, New Zealand, and Iceland. (scielo.org.ar)
- A major development in the recent study of virus tumors has been the demonstration of virus particles in association with tumor cells and the physical identification of ultrastructural cell-virus interrelationships. (chestofbooks.com)
- Levy J. A. Endogenous C - type viruses double agents in natural life proceses. (medkursor.ru)
- Levy J. A. Endogenous С - type viruses in normal and «abnormal» cell development. (medkursor.ru)
- Longstreth J. D., I hie J. N., Banna M. G., Jr. Autogenous immunity to endogenous RNA tumor virus: virion and virusinduced cell surface antigens. (medkursor.ru)
- 1,000 endogenous mRNAs targeted by miRNAs encoded by each virus, many of which are targeted by both MDV-1 and MDV-2 miRNAs. (asm.org)
- The UK's avian disease emerging threats report for the first quarter of 2015 describes cases of enterococcal and streptococcal infections, colibacillosis of several types, and an unusual diagnosis of leucocis in chickens. (thepoultrysite.com)
- These viruses are commonly reported in association with aseptic meningitis outbreaks in pediatric patients ( 1 ). (cdc.gov)
- The H1N2 viruses in France did not spread beyond their initial farms of origin ( 4 ), but the H1N2 viruses in Japan and the United Kingdom caused large-scale outbreaks of disease and spread widely in the swine populations of these countries ( 1 , 2 , 6 , 12 ). (asm.org)
- During the period January to March 2015 outbreaks of H5N8 Highly Pathogenic Avian Influenza (HPAI) were officially reported in Europe in poultry flocks in Germany and Hungary. (thepoultrysite.com)
- Here, we took advantage of an in vitro system for the generation of non-enveloped Mason-Pfizer monkey virus (M-PMV) VLPs derived from a self-assembling CA-NC subunit of Gag. (deepdyve.com)
- In Spain, an outbreak of aseptic meningitis attributable to EV13 occurred from February to October 2000 ( 25 ), the first identification of this virus in Spain since record keeping began in 1988 ( 28 ). (cdc.gov)
- Brugge J. S., Purchio A. F., Erikson R. L. The distribution of virus - specific RNA in RSV - induced hamster tumor cells. (medkursor.ru)
- Aedes aegypti mosquitoes are common vectors for by using second-round amplification with type-specific dengue virus and chikungunya virus. (cdc.gov)
- Aspartic proteinases from human immunodeficiency virus type 1 (HIV-1) and avian myeloblastosis virus (AMV) were found to interfere with microtubule assembly. (nih.gov)
- Among those infective to human, important ones are adult T-cell leukemia virus (ATLV), or human T-lymphotropic virus type I (HTLV-I), and type II (HTLV-II). (google.es)
- Levin R. K., Parks W. P., Scolnick E. M. Expression of feline type - С virus in normal and tumor tissues of the domestic cat. (medkursor.ru)
- Liebermann D., Sachs L. Type С RNA virus production and cell competence for normal differentiation in myeloid leukaemic cells. (medkursor.ru)
- Influenza viruses type A can be further subdivided in different serologically differentiated subtypes according to the structure and composition of two antigenic glycoproteins located in the surface of the virion: haemagglutinin (HA) involved in binding of the virus to host cells and neuraminidase (NA) implicated in release of virus from infected cells. (scielo.org.ar)
- The hemagglutinin (HA) of a recent swine influenza virus, A/Sw/IN/1726/88 (HlNl), was shown previously to have four antigenic sites, as determined from analysis of monoclonal antibody (MAb)-selected escape mutants. (scribd.com)
- Thus, swine influenza viruses evolve more slowly than human viruses, possibly because they are not subjected to the same degree of immune selection. (scribd.com)
- The only means of control when avian influenza has been observed in a flock of domestic fowl is destruction of infected birds and disinfection of the farm. (thefreedictionary.com)
- A disease of birds due to strains of influenza A virus. (thefreedictionary.com)
- Although wild birds, the natural hosts, seldom become sick when infected, avian influenza viruses can cause disease in domestic poultry and, rarely, in human beings. (thefreedictionary.com)
- The virus is capable of spreading from birds to humans. (thefreedictionary.com)
- However, only two equine influenza virus subtypes have been associated with the disease in horses, the H7N7 subtype (equi-1) and the H3N8 subtype (equi-2). (scielo.org.ar)
- Recent studies of the H3N8 subtype of equine influenza viruses have demonstrated that these strains have diverged into two distinct evolutionary lineages (2) . (scielo.org.ar)