Avian myeloblastosis virus: A species of ALPHARETROVIRUS causing anemia in fowl.Avian leukosis virus: The type species of ALPHARETROVIRUS producing latent or manifest lymphoid leukosis in fowl.Avian Leukosis: A group of transmissible viral diseases of chickens and turkeys. Liver tumors are found in most forms, but tumors can be found elsewhere.RNA-Directed DNA Polymerase: An enzyme that synthesizes DNA on an RNA template. It is encoded by the pol gene of retroviruses and by certain retrovirus-like elements. EC 126.96.36.199.Oncogene Proteins v-myb: Transforming proteins coded by myb oncogenes. Transformation of cells by v-myb in conjunction with v-ets is seen in the avian E26 leukemia virus.PolynucleotidesTemplates, Genetic: Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.DNA Nucleotidyltransferases: Enzymes that catalyze the incorporation of deoxyribonucleotides into a chain of DNA. EC 2.7.7.-.Rauscher Virus: A strain of MURINE LEUKEMIA VIRUS associated with mouse tumors similar to those caused by the FRIEND MURINE LEUKEMIA VIRUS. It is a replication-competent murine leukemia virus. It can act as a helper virus when complexing with a defective transforming component, RAUSCHER SPLEEN FOCUS-FORMING VIRUS.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Streptonigrin: Complex cytotoxic antibiotic obtained from Streptomyces flocculus or S. rufochronmogenus. It is used in advanced carcinoma and causes leukopenia.RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Thymine Nucleotides: Phosphate esters of THYMIDINE in N-glycosidic linkage with ribose or deoxyribose, as occurs in nucleic acids. (From Dorland, 28th ed, p1154)Avian Sarcoma Viruses: Group of alpharetroviruses (ALPHARETROVIRUS) producing sarcomata and other tumors in chickens and other fowl and also in pigeons, ducks, and RATS.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Alpharetrovirus: A genus of the family RETROVIRIDAE with type C morphology, that causes malignant and other diseases in wild birds and domestic fowl.Chick Embryo: The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.TritiumSatellite Viruses: Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.Centrifugation, Density Gradient: Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Ribonucleases: Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-.Oncogenes: Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.Cell-Free System: A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)Molecular Weight: The sum of the weight of all the atoms in a molecule.DNA-Directed DNA Polymerase: DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.Phosphotungstic Acid: Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Cell Transformation, Viral: An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.Viral Proteins: Proteins found in any species of virus.Oncogenic Viruses: Viruses that produce tumors.Retroviridae: Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).Moloney murine leukemia virus: A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 mouse sarcoma, and causing lymphoid leukemia in mice. It also infects rats and newborn hamsters. It is apparently transmitted to embryos in utero and to newborns through mother's milk.Cytosine NucleotidesPoly C: A group of cytosine ribonucleotides in which the phosphate residues of each cytosine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Deoxyribonucleotides: A purine or pyrimidine base bonded to a DEOXYRIBOSE containing a bond to a phosphate group.Reverse Transcriptase Inhibitors: Inhibitors of reverse transcriptase (RNA-DIRECTED DNA POLYMERASE), an enzyme that synthesizes DNA on an RNA template.Genes, Viral: The functional hereditary units of VIRUSES.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Helper Viruses: Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.RNA, Transfer: The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.Kinetics: The rate dynamics in chemical or physical systems.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Retroviridae Proteins, Oncogenic: Retroviral proteins that have the ability to transform cells. They can induce sarcomas, leukemias, lymphomas, and mammary carcinomas. Not all retroviral proteins are oncogenic.Manganese: A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)Ribonuclease H: A ribonuclease that specifically cleaves the RNA moiety of RNA:DNA hybrids. It has been isolated from a wide variety of prokaryotic and eukaryotic organisms as well as RETROVIRUSES.Proto-Oncogene Proteins c-myb: Cellular DNA-binding proteins encoded by the myb gene (GENES, MYB). They are expressed in a wide variety of cells including thymocytes and lymphocytes, and regulate cell differentiation. Overexpression of myb is associated with autoimmune diseases and malignancies.Yolk Sac: The first of four extra-embryonic membranes to form during EMBRYOGENESIS. In REPTILES and BIRDS, it arises from endoderm and mesoderm to incorporate the EGG YOLK into the DIGESTIVE TRACT for nourishing the embryo. In placental MAMMALS, its nutritional function is vestigial; however, it is the source of INTESTINAL MUCOSA; BLOOD CELLS; and GERM CELLS. It is sometimes called the vitelline sac, which should not be confused with the VITELLINE MEMBRANE of the egg.Retroviridae Proteins: Proteins from the family Retroviridae. The most frequently encountered member of this family is the Rous sarcoma virus protein.Endonucleases: Enzymes that catalyze the hydrolysis of the internal bonds and thereby the formation of polynucleotides or oligonucleotides from ribo- or deoxyribonucleotide chains. EC 3.1.-.Nucleotides: The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.DNA, Single-Stranded: A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.Poly A: A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Magnesium: A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Integrases: Recombinases that insert exogenous DNA into the host genome. Examples include proteins encoded by the POL GENE of RETROVIRIDAE and also by temperate BACTERIOPHAGES, the best known being BACTERIOPHAGE LAMBDA.Phosphorus Isotopes: Stable phosphorus atoms that have the same atomic number as the element phosphorus, but differ in atomic weight. P-31 is a stable phosphorus isotope.Cell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Deoxyribonucleases: Enzymes which catalyze the hydrolases of ester bonds within DNA. EC 3.1.-.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Transformation by v-Myb. (1/401)The v-myb oncogene of the avian myeloblastosis virus (AMV) is unique among known oncogenes in that it causes only acute leukemia in animals and transforms only hematopoietic cells in culture. AMV was discovered in the 1930s as a virus that caused a disease in chickens that is similar to acute myelogenous leukemia in humans (Hall et al., 1941). This avian retrovirus played an important role in the history of cancer research for two reasons. First, AMV was used to demonstrate that all oncogenic viruses did not contain a single cancer-causing principle. In particular, although both Rous sarcoma virus (RSV) and AMV could replicate in cultures of either embryonic fibroblasts or hematopoietic cells, RSV could transform only fibroblasts whereas AMV could transform only hematopoietic cells (Baluda, 1963; Durban and Boettiger, 1981a). Second, chickens infected with AMV develop remarkably high white counts and therefore their peripheral blood contains remarkably large quantities of viral particles (Beard, 1963). For this reason AMV was often used as a prototypic retrovirus in order to study viral assembly and later to produce large amounts of reverse transcriptase for both research and commercial purposes. Following the discovery of the v-src oncogene of RSV and the demonstration that it arose from the normal c-src proto-oncogene, a number of acute leukemia viruses were analysed by similar techniques and found to also contain viral oncogenes of cellular origin (Roussel et al., 1979). In the case of AMV, it was shown that almost the entire retroviral env gene had been replaced by a sequence of cellular origin (initially called mab or amv, but later renamed v-myb) (Duesberg et al., 1980; Souza et al., 1980). Remarkably, sequences contained in this myb oncogene were shared between AMV and the avian E26 leukemia virus, but were not contained in any other acutely transforming retroviruses. In addition, the E26 virus contained a second sequence of cellular origin (ets) that was unique. The E26 leukemia virus was first described in the 1960s and causes an acute erythroblastosis in chickens, more reminiscent of the disease caused by avian erythroblastosis virus (AEV) than by AMV (Ivanov et al., 1962). (+info)
Retrovirus DNA termini bound by integrase communicate in trans for full-site integration in vitro. (2/401)Integration of linear retrovirus DNA involves the concerted insertion of the viral termini (full-site integration) into the host chromosome. We investigated the interactions that occur between long terminal repeat (LTR) termini bound by avian retrovirus integrase (IN) for full-site integration in vitro. Wild-type (wt) or mutant LTR donors that possess gain-of-function ("G") or loss-of-function ("L") for full-site integration activity were used. G LTR termini are characterized as having significantly higher strand transfer activity than the wt and the L LTR termini. L LTR mutations are classified as partially or extremely defective for strand transfer activity. The L mutations were further classified by their ability to either permit or block the assembly of G or wt LTR termini into nucleoprotein complexes capable of full-site strand transfer. We demonstrated that avian myeloblastosis virus IN bound to G LTR termini increased the incorporation of partially defective L LTR termini into nucleoprotein complexes that were capable of full-site integration. The observed full-site integration activity of these assembled nucleoprotein complexes appeared to be influenced by each individual IN-LTR complex in trans. In contrast, extremely defective L LTR termini exhibited the ability to effectively block the assembly of wt LTR termini into nucleoprotein complexes capable of full-site strand transfer. Data from nonspecific DNA competition experiments suggested that IN had an apparent higher affinity for G LTR donor termini than for partially defective L LTR donor termini as measured by full-site integration activity. However, assembled nucleoprotein complexes containing either two G or two L LTR donors were stable, having a similar half-life of approximately 2 h on ice. The results suggest that LTR termini bound by IN exhibit an allosteric effect to modulate full-site integration in vitro. Similar regulatory controls also appear to exist in vivo between the wt U3 and wt U5 LTR termini in retroviruses as well as purified retrovirus preintegration complexes that promoted full-site integration in vitro. (+info)
Retinoid X receptor suppresses transformation by the v-myb oncogene. (3/401)The v-myb oncogene of avian myeloblastosis virus causes acute monoblastic leukemia in vivo and transforms myelomonocytic cells in culture. Retinoids are potent regulators of proliferation and differentiation in various cell types, and they can initiate differentiation in certain types of leukemic cells. However, the BM2 v-myb-transformed chicken monoblastic cell line is resistant to retinoic acid treatment. We found that overexpression of the retinoid X receptor confers sensitivity of BM2 cells to retinoic acid, resulting in induction of growth arrest and terminal differentiation. In contrast, the frequency of apoptosis was not affected by the retinoid X receptor in this cell type. We also demonstrated that suppression of transformation by v-Myb results from the negative effect of retinoid X receptor on v-Myb transactivation function, similar to that previously described for the retinoic acid receptor. The retinoid X receptor-induced inhibition of transactivation by v-Myb seems to be enhanced by a cell type-specific factor(s), which is not required by retinoic acid receptor. (+info)
Metal-induced infidelity during DNA synthesis. (4/401)The effect of several divalent cations on the accuracy of DNA replication in vitro has been examined. Only Be2+ altered the accuracy of DNA synthesis using purified DNA polymerase (DNA nucleotidyltransferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase; EC 188.8.131.52) from avian myeloblastosis virus. The Be2+-induced base substitutions occurred with all templates and with all nucleotides tested. Analysis of the product by equilibrium density centrifugation and processive hydrolysis with snake venom phosphodiesterase suggested that the noncomplementary nucleotides were present in phosphodiester linkage. Nearest neighbor studies indicated that many of the Be2+-induced errors were present as single base substitutions. The enhancement of error frequency could be duplicated by the pretreatment of the enzyme, but not the template, with Be2+. Glycerol gradient centrifugation dissociated the Be2+-DNA polymerase complex and restored the initial error frequency of the polymerase. Thus, the weak binding of a metal cation to a DNA polymerase could alter the accuracy with which that polymerase copied DNA. Beryllium is a known carcinogen. The potential use of this system as a screening technique to detect chemical mutagens and carcinogens is considered. (+info)
Chromatin as a template for RNA synthesis in vitro. (5/401)RNA transcribed in vitro from myeloblast chromatin by exogenously added RNA polymerase B predominantly consists of short chains that remain in hybrid structure with the template; the remainder of the product is free RNA of heterogeneous size. Addition of polyanions during synthesis caused an increase in the size and amount of free RNA with a concomitant decrease in the proportion of small RNA. The large molecular weight RNA is derived from the short RNA chains, which are synthesized de novo during the reaction in vitro. The effect of polyanions on the size and nature of the product may be related to structural changes induced in the template rather than to an inhibition of nuclease activity. (+info)
A new chemical procedure for 32P-labeling of ribonucleic acids at their 5'-ends after isolation. (6/401)A new technique, which utilizes the chemical reaction between [32P]diimidazolidate of orthophosphate and the cetyltrimethylammonium salt of high-molecular-weight RNA in nonaqueous dimethyl formamide, has been developed for the 32P-labeling of RNAs after isolation. The radioactive label of high specific activity is introduced onto a phosphorylated 5'-end of the RNA and renders it suitable for 5'-terminal group analysis. When the labeling reaction was applied to the 70S RNA of avian myeloblastosis virus, a labeled 35S RNA was isolated on sucrose-dimethyl sulfoxide gradients without apparent degradation. (+info)
Avian retrovirus DNA internal attachment site requirements for full-site integration in vitro. (7/401)Concerted integration of retrovirus DNA termini into the host chromosome in vivo requires specific interactions between the cis-acting attachment (att) sites at the viral termini and the viral integrase (IN) in trans. In this study, reconstruction experiments with purified avian myeloblastosis virus (AMV) IN and retrovirus-like donor substrates containing wild-type and mutant termini were performed to map the internal att DNA sequence requirements for concerted integration, here termed full-site integration. The avian retrovirus mutations were modeled after internal att site mutations studied at the in vivo level with human immunodeficiency virus type 1 (HIV-1) and murine leukemia virus (MLV). Systematic overlapping 4-bp deletions starting at nucleotide positions 7, 8, and 9 in the U3 terminus had a decreasing detrimental gradient effect on full-site integration, while more internal 4-bp deletions had little or no effect. This decreasing detrimental gradient effect was measured by the ability of mutant U3 ends to interact with wild-type U3 ends for full-site integration in trans. Modification of the highly conserved C at position 7 on the catalytic strand to either A or T resulted in the same severe decrease in full-site integration as the 4-bp deletion starting at this position. These studies suggest that nucleotide position 7 is crucial for interactions near the active site of IN for integration activity and for communication in trans between ends bound by IN for full-site integration. The ability of AMV IN to interact with internal att sequences to mediate full-site integration in vitro is similar to the internal att site requirements observed with MLV and HIV-1 in vivo and with their preintegration complexes in vitro. (+info)
Modeling the late steps in HIV-1 retroviral integrase-catalyzed DNA integration. (8/401)Model oligodeoxyribonucleotide substrates representing viral DNA integration intermediates with a gap and a two-nucleotide 5' overhang were used to examine late steps in human immunodeficiency virus, type 1 (HIV-1) retroviral integrase (IN)-catalyzed DNA integration in vitro. HIV-1 or avian myeloblastosis virus reverse transcriptase (RT) were capable of quantitatively filling in the gap to create a nicked substrate but did not remove the 5' overhang. HIV-1 IN also failed to remove the 5' overhang with the gapped substrate. However, with a nicked substrate formed by RT, HIV-1 IN removed the overhang and covalently closed the nick in a disintegration-like reaction. The efficiency of this closure reaction was very low. Such closure was not stimulated by the addition of HMG-(I/Y), suggesting that this protein only acts during the early processing and joining reactions. Addition of Flap endonuclease-1, a nuclease known to remove 5' overhangs, abolished the closure reaction catalyzed by IN. A series of base pair inversions, introduced into the HIV-1 U5 long terminal repeat sequence adjacent to and/or including the conserved CA dinucleotide, produced no or only a small decrease in the HIV-1 IN-dependent strand closure reaction. These same mutations caused a significant decrease in the efficiency of concerted DNA integration by a modified donor DNA in vitro, suggesting that recognition of the ends of the long terminal repeat sequence is required only in the early steps of DNA integration. Finally, a combination of HIV-1 RT, Flap endonuclease-1, and DNA ligase is capable of quantitatively forming covalently closed DNA with these model substrates. These results support the hypothesis that cellular enzyme(s) may catalyze the late steps of retroviral DNA integration. (+info)
William A. Haseltine
"Specific Binding of Tryptophan Transfer RNA to Avian Myeloblastosis Virus Reverse Transcriptase". Proceedings of the National ... They proposed to use methods similar to those used to isolate the HTLV virus to find this new virus. These methods were ... "Gibbon Ape Leukemia Virus Hall's Island: New Strain of Gibbon Ape Leukemia Virus". Journal of Virology. 29 (1): 395-400. PMC ... The laboratory also created hybrid viruses that carry some monkey and some HIV genes-the so-called SHIV viruses-so that new ...
Species include the Rous sarcoma virus, avian leukosis virus, and avian myeloblastosis virus. Alpharetrovirus at the US ...
AMV reverse transcriptase from the avian myeloblastosis virus also has two subunits, a 63 kDa subunit and a 95 kDa subunit. ... Konishi A, Yasukawa K, Inouye K (2012). "Improving the thermal stability of avian myeloblastosis virus reverse transcriptase α- ... VIRUSES > F. ANIMAL VIRUS LIFE CYCLES > 3. The Life Cycle of HIV Community College of Baltimore County. Updated: Jan 2008. ... Retroviruses, also referred to as class VI ssRNA-RT viruses, are RNA reverse-transcribing viruses with a DNA intermediate. ...
Antimicrobial properties of copper
Vasudevachari, M; Antony, A (1982). "Inhibition of avian myeloblastosis virus reverse transcriptase and virus inactivation by ... The result is inactivation of bacteria or viruses Copper complexes form radicals that inactivate viruses. Copper may disrupt ... Influenza A virus was found to survive in large numbers on stainless steel. Once surfaces are contaminated with virus particles ... These viruses are a frequent cause of diarrhea. In a recent study, 75% of adenovirus particles were inactivated on copper ( ...
This region gets its name from a viral protein called Myb derived from the avian myeloblastosis virus. Specifically, the ...
Maurice Green (virologist)
Grandgenett, D. P.; Gerard, G. F.; Green, M. (1973). "A Single Subunit from Avian Myeloblastosis Virus with Both RNA-Directed ... Later, Green's research extended to the RNA tumor viruses (tumor inducing viruses with an RNA genome). Before reverse ... His studies included characterizing the viruses' DNA, investigating the tumor-inducing properties of the viruses, and ... workers conducted important studies on the biochemical features of reverse transcriptases of avian and murine RNA tumor viruses ...
... a Finnish military vehicle Avian myeloblastosis. ... a plant virus of the family Bromoviridae All Mobile Video, a ... a British advertising agency Alfalfa mosaic virus, ...
List of virus species
... virus 2 Avian coronavirus Avian dependoparvovirus 1 Avian leukosis virus Avian metapneumovirus Avian myeloblastosis virus Avian ... virus A Potato virus H Potato virus M Potato virus P Potato virus S Potato virus T Potato virus U Potato virus V Potato virus X ... virus Garlic virus A Garlic virus B Garlic virus C Garlic virus D Garlic virus E Garlic virus X Gayfeather mild mottle virus ... virus Vibrio virus 493 Vibrio virus CTXphi Vibrio virus fs1 Vibrio virus fs2 Vibrio virus K139 Vibrio virus Kappa Vibrio virus ...
জৈৱ প্ৰযুক্তি - অসমীয়া ৱিকিপিডিয়া
Rous Sarcoma virus/ RSV, Mouse Mammary tumour virus/MMTV, Avian Myeloblastosis Virus/AMV আৰু Murine Leukaemia Virus/MuLV) পৰা ...
List of MeSH codes (B04)
... avian MeSH B04.820.650.070.500 --- leukosis virus, avian MeSH B04.820.650.070.550 --- myeloblastosis virus, avian MeSH B04.820. ... avian MeSH B04.909.574.807.070.500 --- leukosis virus, avian MeSH B04.909.574.807.070.550 --- myeloblastosis virus, avian MeSH ... avian MeSH B04.909.777.731.070.500 --- leukosis virus, avian MeSH B04.909.777.731.070.550 --- myeloblastosis virus, avian MeSH ... yellow fever virus MeSH B04.820.250.400 --- gb virus a MeSH B04.820.250.405 --- gb virus b MeSH B04.820.250.410 --- GB virus C ...
Taxonomic list of viruses
Avian carcinoma Mill Hill virus 2 Avian leukosis virus Avian myeloblastosis virus Avian myelocytomatosis virus 29 Avian sarcoma ... virus Shuni virus Simbu virus Tacaiuma virus Tete virus Thimiri virus Timboteua virus Turlock virus Wyeomyia virus Zegla virus ... virus Uganda S virus Usutu virus Wesselsbron virus West Nile virus Yaounde virus Yellow fever virus Yokose virus Zika virus ... Capim virus Caraparu virus Catu virus Estero Real virus Gamboa virus Guajara virus Guama virus Guaroa virus Kaeng Khoi virus ...
In addition to neoplasias caused in chickens by helper viruses of the avian myeloblastosis virus (AMV) complex, acute myeloblastic leukemia is induced by a defective leukemogenic component. To...
Avian myeloblastosis ATCC ® VR-1542AS-Gt™ Designation: antiserum against AMV RT [NCI HE 599] Application: goat antiserum against the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT)
Rosok, Mae Joanne, "Dissociation and isolation of the subunits of avian myeloblastosis virus RNA-directed DNA polymerase" (1977). Graduate Student Theses, Dissertations, & Professional Papers. 6828 ...
The avian myeloblastosis virus (AMV) is an alpha retrovirus responsible for acute myeloblastic leukemia (AML) when injected in ovo, or in newly hatched chickens . Early in vitro dose response experiments indicated that the production of virion with leukemogenic potential required a double infection with AMV and a helper virus . The AMV strains that are used and commercially available, are derived from the orignal BAI strain A purified from chicken leukemic plasma . Leukemic plasma containing the BAI strain has been widely distributed for many years by Life Sciences Inc., in Florida which has been the official provider of national agencies in the USA. The Standard AMV-S BAI strain is a complex mixture of viruses that also includes two helper viruses in addition to AMV. The helper viruses Myeoloblastosis Associated Virus (MAV) contained in AMV-S belong to two different serological subgoups (type1 and type2, also called A and B). Both of them are oncogenic .. Both MAV-1 and MAV-2 ...
Immune Stimulation of Sensitized Chicken Lymphocytes by Avian Retrovirus Proteins | Microbiology Society
Summary Peripheral blood lymphocytes of chickens bearing tumours induced by avian sarcoma virus can be specifically stimulated to divide by the crude culture fluids of virus-infected cells. In this communication, we show that relevant antigenic activity apparently resides in each of the internal virus proteins p15 and p27. The ability of infectious culture fluids to be mitogenic for sensitized lymphocytes is greatly reduced following treatment with antibodies specific for either total avian myeloblastosis virus (AMV) protein or for p27.
Primers 1 and 2 were designed from the mRNA of a guinea pig BK B2 receptor (GenBank accession no. AJ003243) to amplify a 500-base pair fragment. Primers 3 and 4 were a pair of degenerate primers used to amplify a 401-base pair fragment for the guinea pig BK B1 receptor. Primers 3 and 4 were made from the conserved region between mouse BK B1 receptor (GenBank accession no. NM_007539) and rat BK B1 receptor (GenBank accession no. U66107). Amplification was done with an iCycler (Bio-Rad, Hercules, CA). PCR cycles consisted of denaturation for 1 min at 94°C, annealing at 56°C for 90 s, and extension at 72°C for 90 s. A total of 30 cycles was followed by completion of extension for 7 min at 72°C. PCR product (12 μl) was analyzed by electrophoresis on 2% agarose gel. Sequencing was done with an ABI 373XL DNA sequencer after the PCR product was purified with Qiaquick PCR purification kit (QIAGEN, Valencia, CA). RT-PCR without avian myeloblastosis virus reverse transcriptase was used as a negative ...
RT Components: 1U/mL RNase inhibitor, 0.625U/mL reverse transcriptase (avian myeloblastosis virus, Boehringer), 1microM G1 primer, 1.75mM dNTPs ...
Compare v-myb myeloblastosis viral oncogene homolog (avian)-like 2 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
MYB overexpression lysate, 0.1 mg. Transient overexpression lysate of v-myb myeloblastosis viral oncogene homolog (avian) (MYB), transcript variant 2
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The effect of negative stain, freeze-fracture, and thin-sectioning procedures on the size of several oncornaviruses has been determined. After negative staining, the average outer dimensions measured over several experiments for the C-type avian viruses, avian myeloblastosis virus (AMV) and Rous sarcoma virus, Prague strain, were about 1370 and 1340 Å, respectively. These values were substantially smaller than the outer dimensions of the C-type murine viruses, Friend leukemia virus (FLV), namely, 1470 Å, and Moloney leukemia virus, namely, 1460 Å. The avian viruses were similar in size to the B-type murine virus, mouse mammary tumor virus, which measured about 1290 Å. All experimental values were obtained after glutaraldehyde (1.5%) fixation and uranyl acetate (2%, pH 4.2) staining. Other negative stains, such as sodium phosphotungstate (1%, pH 6.6), lead to disruption of the outer envelope and a concomitant diminution in size. Freeze-fractured preparations of AMV showed particles with or ...
The diversity and polymorphisms in the ToMV population present in one ToMV-positive plant per genotype was further assessed. Total RNAs were isolated from the upper leaf of plants at 21 and 48 d.p.i. The complete ToMV ORF4 sequence was amplified using a two-step reverse-transcriptase polymerase chain reaction (RT-PCR) protocol. The first-strand cDNA was synthesized by reverse transcription of total RNAs using random hexamer primers and the Avian myeloblastosis virus reverse transcriptase (Promega Corp., Madison, WI, USA). A 480-bp fragment (corresponding to positions 5703-6182 of the complete ToMV reference sequence, NC_002692) was amplified using primer pair ToMV-F (5′-ATGTCTTACTCAATCACTTC-3′, sense) and ToMV-R (5′-TTAAGATGCAGGTGCAGAGG-3′, antisense) and the TaKaRa LA Taq polymerase (TaKaRa, Bio Inc., Shiga, Japan). PCR amplification was performed under the following cycling conditions: initial denaturation at 94°C for 5 min; 25 cycles of 94°C for 45 s, 54°C for 30 s, and 72°C for ...
Akt Inhibitor MK2206 in Treating Patients With Progressive, Recurrent, or Metastatic Adenoid Cyst Carcinoma - Full Text View -...
PRIMARY OBJECTIVES:. I. To determine the confirmed response rate of patients with progressive, recurrent/metastatic adenoid cyst carcinoma (ACC) treated with v-akt murine thymoma viral oncogene homolog 1 (Akt) inhibitor MK2206 (MK-2206).. SECONDARY OBJECTIVES:. I. To evaluate the progression-free survival (PFS), overall survival (OS), and safety/tolerability for MK-2206 in these patients.. TERTIARY OBJECTIVES:. I. To explore potential genetic/cytogenetic/histopathologic predictors of clinical outcome (i.e., response, PFS, OS) to MK-2206.. II. To explore the hypothesis that MK-2206-mediated Akt inhibition and downregulation of v-myb avian myeloblastosis viral oncogene homolog (c-myb) protein levels in ACC tumors correlates to clinical outcome (i.e., response, PFS, OS).. OUTLINE:. Patients receive Akt inhibitor MK2206 orally (PO) once weekly for 4 weeks. Courses repeat every 28 days in the absence of disease progression or unacceptable toxicity.. After completion of treatment, patients are ...
During the late 1960s and early 1970s, cell tumor biology researchers determined that synthetic RNA and feline leukaemia virus (FELV) "template" added to "human type C" viruses--those associated with cancers of the lymph nodes increased the rate of DNA production (and subsequent provirus and virus reproduction) as much as thirty times.(1) Such hybrid viruses, these researchers reported, may cause many cancers besides leukaemias and lymphomas, including sarcomas. Other NCI and Litton Bionetics teams reported modifying the fortieth discovered simian virus (SV40) by infusing it with nucleic acids from other species including FELV RNA, avian (i.e., chicken) myeloblastosis virus (AMV) RNA, associated with leukemia and sarcoma development, and mouse sarcoma RNA to: 1) make them carcinogenic, 2) prompt extreme immunosuppression in primates,(2,4,11) and 3) study RNA-dependent DNA polymerase (i.e., reverse transcriptase) and its relationship to human carcinogenesis,(6,11-14) For example, early work in ...
The AMV First Strand cDNA Synthesis Kit combines AMV Enzyme Mix and AMV Reaction Mix. The AMV Enzyme Mix is an optimized blend of AMV Reverse Transcriptase and a Murine RNase Inhibitor. AMV Reverse Transcriptase provides first strand cDNA synthesis reactions with a broader optimal reaction temperature from 37°C to 50°C
Reverse transcriptases can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template.
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Looking for online definition of nephroblastomas in the Medical Dictionary? nephroblastomas explanation free. What is nephroblastomas? Meaning of nephroblastomas medical term. What does nephroblastomas mean?
The amplification of RNA requires the conversion of the RNA substrate into DNA. This is achieved through the use of a reverse transcriptase such as AMV RT (avian myeloblastis virus reverse transcriptase) or M-MuLV RT (moloney murine leukemia virus reverse transcriptase). The resulting cDNA can be used as a template for a standard PCR. Nested PCR means that two pairs of PCR primers were used for a single locus. The first pair generates an amplicon within the locus as seen in any PCR experiment. The second pair of primers (nested primers) bind within the first amplicon and produce a second PCR product that will be shorter than the first one. The logic behind this strategy is that if the wrong locus were amplified by mistake, the probability is very low that it would also be amplified a second time by a second pair of primers. ...
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nephroblastoma: Malignant renal (kidney) tumour of early childhood. In 75 percent of the cases, the tumour grows before the age of five; about two-thirds of the instances are apparent by two years...
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Retrovirus- associated DNA sequences ( v- myb) originally isolated from the avian myeloblastosis and E26 leukemia viruses. The proto- oncogene c- myb codes for a nuclear protein involved in transcriptional regulation and appears to be essential for hematopoietic cell proliferation. The human myb gene is located at 6q22- 23 on the short arm of chromosome 6. This is the point of break in translocations involved in T- cell acute lymphatic leukemia and in some ovarian cancers and melanomas. ( From Ibelgaufts, Dictionary of Cytokines, 1995 ...
NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse...
SVEC4-10 and MEF cells (2 × 106) were mock-infected or infected with WT or Δm145 MCMV (1 and 0.5 PFU/cell, respectively). Upon 12 h of infection, the cells were collected and total RNA was isolated by TriPure reagent (Roche Diagnostics) according to the manufacturers instruction. cDNA was synthetized from 1 μg of total RNA by using the 1st Strand cDNA Synthesis Kit for RT-PCR (Roche Diagnostics, containing: oligo (dT)15 primers (0.04 U A260), 1 mM dNTPs, 5 mM MgCl2, 2.5 U/μl RNase inhibitor, AMV RT buffer (×1), and 1 U/μl AMV reverse transcriptase; final volume of reaction, 20 μl) under the following conditions: 10 min at 25°C, 1 h at 42°C, 10 min at 95°C, and 10 min at 4°C. The cDNA was diluted 1:5 in water and 2 μl was used for each quantitative real-time PCR reaction designed to measure the number of MULT-1 and β-actin cDNA copies in each sample and performed on LightCycler (Roche Diagnostics). The MULT-1 cDNA PCR product (185 bp) was amplified by using exon spanning MULT-F ...
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The present study reviews 31 patients with histologically confirmed nephroblastoma seen over a 5-year period (1985-1989). There was a predominance of female patients with a male: female ratio of 1:1.7, and the average age of the patients was 47 month
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Looking for online definition of oncornavirus in the Medical Dictionary? oncornavirus explanation free. What is oncornavirus? Meaning of oncornavirus medical term. What does oncornavirus mean?
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Diagnostics of nephroblastoma (Wilms tumor): Costs for treatment #206387 in Switzerland | BookingHealth
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Positive Tested Species Reactivity: Avian-myoblastosis-virus-%28AMV%29, Broad-species, Recommended Applications: Function...
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
ENGLISH ABSTRACT: Introduction and purpose The aim of the study was to determine the renal handling of a once-off bolus dietary protein load in patients treated for nephroblastoma. Patients who have been managed for nephroblastoma always have suboptimal amounts of kidney tissue as a result of their medical management which includes nephrectomies, chemotherapy and or radiotherapy. Little data are available indicating the extent of renal impairment expected in such patients as a result of their disease and management. The study was to determine whether the use of regular screening tests such as serum urea, creatinine and urine microalbumin, in conjunction with a dietary protein load could help detect early progressive deterioration of kidney function in nephroblastoma patients. Methodology The study was a quantitative non-randomised intervention study in which patients served as their own control before and after a protein load. Thirty-four participants were included in the study. Each participant ...
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Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
In medicine, the suffix -blast refers to immature, precursor cells or stem cells. These cells are important in disease detection. Learn more.
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Mutations in human and/or mouse homologs are associated with this disease. Synonyms: adult nephroblastoma; adult renal Wilms tumor; childhood renal Wilms tumor; childhood renal Wilms cancer; renal Wilms tumor; Wilms tumor
Here we present data showing that GoScript Reverse Transcriptase performs as well as or better than competitor reverse transcriptases in terms of sensitivity, transcript length produced and sensitivity to ethanol contamination.
Identification of the Avian Myeloblastosis Virus Genome | Springer for Research & Development
In addition to neoplasias caused in chickens by helper viruses of the avian myeloblastosis virus (AMV) complex, acute ... In addition to neoplasias caused in chickens by helper viruses of the avian myeloblastosis virus (AMV) complex, acute ... Souza L.M., Bergmann D.G., Baluda M.A. (1981) Identification of the Avian Myeloblastosis Virus Genome. In: Neth R., Gallo R.C ... Silva RF, Baluda MA (to be published) Avian myeloblastosis virus proteins in leukemic chicken myeloblasts. J VirolGoogle ...https://rd.springer.com/chapter/10.1007/978-3-642-67984-1_78
Avian myeloblastosis ATCC ® VR-1542AS-Gt™
... goat antiserum against the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) ... Avian myeloblastosis ATCC ® VR-1542AS-Gt™ Designation: antiserum against AMV RT [NCI HE 599] Application: ... Avian myeloblastosis (ATCC® VR-1542AS-Gt™) Classification: Retrovirus, Avian Type C Retrovirus Group / Product Format: frozen ... goat antiserum against the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT). The antiserum was prepared by ...https://www.atcc.org/en/Products/Cells_and_Microorganisms/Viruses/Antisera_and_Monoclonal_Antibodies/VR-1542AS-Gt.aspx
Identification and Regulation of c-Myb Target Genes in MCF-7 Cells | BMC Cancer | Full Text
... shown that overexpression of the normal c-Myb protein or the oncogenic derivative v-Myb protein from Avian Myeloblastosis Virus ... The c-myb gene is a cellular proto-oncogene from which the v-myb oncogenes expressed by two avian leukemia viruses are derived ... along with the lentiviral packaging plasmid delta 8.9 and the pMD.G plasmid expressing the vesicular stomatitis virus ...https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-30
Avian Leukosis, Lymphoid Leukosis, Leukosis/Sarkoma Group - El Sitio Avicola
The causative viruses are rapidly inactivated at ambient temperature and on exposure to most disinfectants. Signs. *Depression ... A complex of viral diseases with various manifestations such as lymphoid leukosis, myeloblastosis (see Sero-type J), ... Avian Leukosis, Lymphoid Leukosis, Leukosis/Sarkoma Group Extracted From: A Pocket Guide to ...http://www.elsitioavicola.com/diseaseinfo/11/avian-leukosis-lymphoid-leukosis-leukosis-sarkoma-group/
Gag polyprotein - Avian myeloblastosis virus
Avian myeloblastosis-associated virus 1/2. Avian myeloblastosis-associated virus type 2. Avian leukosis virus HPRS103. Avian ... Rous sarcoma virus. Avian leukosis and sarcoma virus. Avian myeloblastosis-associated virus type 1. Avian leukosis virus ... Avian endogenous virus EV-1. Avian leukosis virus (ALV). Rous-associated virus type 2. Avian leukemia virus. Avian leukosis ... Avian endogenous virus EV-1. Avian leukosis virus (ALV). Rous-associated virus type 2. Gallus gallus (Chicken). Avian leukosis ...http://www.uniprot.org/uniprot/Q86830
Antigenic variation of the Avian Myeloblastosis Virus obtained from chick embryo fibroblasts | SpringerLink
Immunelektrophoretisch wird ein verschiedenes Verhalten des Vogel-Myeblastosis-Virus nachgewiesen, je nachdem, ob dieses in ... Chick Embryo Antigenic Variation Avian Myeloblastosis Virus Chick Embryo Fibroblast This work was done while the author was at ... Antigenic variation of the Avian Myeloblastosis Virus obtained from chick embryo fibroblasts. ... Immunelektrophoretisch wird ein verschiedenes Verhalten des Vogel-Myeblastosis-Virus nachgewiesen, je nachdem, ob dieses in ...https://link.springer.com/article/10.1007/BF01946182
Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase) ...
Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase) ... A complex of tRNATrp and the avian myeloblastosis virus reverse transcriptase has been demonstrated using chromatography on ... Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase). ... The ability of tryptophan tRNA (tRNATrp) to initiate reverse transcription of the 70S RNA of avian RNA tumor viruses suggested ...https://authors.library.caltech.edu/7630/
AMV Reverse Transcriptase, recombinant, GMP Grade from Avian Myeloblastosis Virus, expressed in E. coli
AMV Reverse Transcriptase, recombinant, GMP Grade from Avian Myeloblastosis Virus, expressed in ,i,E. coli,/i, ...http://custombiotech.roche.com/home/Product_Details/3_6_14_3_8_2.product-classes%5Call-products.html
AMV Reverse Transcriptase, recombinant, GMP Grade from Avian Myeloblastosis Virus, expressed in E. coli
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Molecular Analysis of Echovirus 13 Isolates and Aseptic Meningitis, Spain - Volume 9, Number 8-August 2003 - Emerging...
... of the neutralizing antigenic site III has been described in this genome region in poliovirus and swine vesicular disease virus ... 1 U of avian myeloblastosis virus RT; and 1 U of Thermus flavus DNA polymerase and Rnase-free distilled water to a final volume ... Virus taxonomy. Seventh report of the International Committee on Taxonomy on Viruses. San Diego: Academic Press; 2000. ... The HEV-B cluster includes coxsackie virus B (CBV), coxsackie virus A9, ENV69, and all echoviruses (EV). ...https://wwwnc.cdc.gov/eid/article/9/8/03-0080_article
Transcriptional Control of the Hydrogen Cyanide Biosynthetic Genes hcnABC by the Anaerobic Regulator ANR and the Quorum-Sensing...
Avian myeloblastosis virus reverse transcriptase (30 U; Pharmacia) was used to extend the primer in a reaction mixture ... infection with human immunodeficiency virus, or severe burn wounds (16, 29). The pathogenesis of P. aeruginosa infections is ... in Pseudomonas fluorescens strain P3 improve the induction of systemic resistance in tobacco against tobacco necrosis virus. ...https://jb.asm.org/content/182/24/6940?ijkey=19be0c3c2c186e70b498d0cdaada6ca1b7359739&keytype2=tf_ipsecsha
Antisera and Monoclonal Antibodies Page 8
goat antiserum against the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT). * Product Sheet ... Avian myeloblastosis (ATCC® VR-1542AS-Gt™) ATCC® Number: VR-1542AS-Gt™ Classification: Retrovirus, Avian Type C Retrovirus ... Influenza A virus (ATCC® VR-1287™) ATCC® Number: VR-1287™ Product Format: freeze-dried ... Lone star virus (ATCC® VR-1230AF™) ATCC® Number: VR-1230AF™ Classification: Bunyaviridae ...https://www.atcc.org/en/Products/Cells_and_Microorganisms/Viruses/Antisera_and_Monoclonal_Antibodies.aspx?dsNav=Ro:70,Up:Page_Product_Listing&slp=1
Activity of a Chick Collagen Gene in Heterologous and Homologous Cell-Free Extracts | SpringerLink
Collagen Gene Rous Sarcoma Virus Avian Myeloblastosis Virus Chick Embryo Fibroblast Nuclease Mapping ...https://link.springer.com/chapter/10.1007/978-1-4684-7459-6_10
Patent US4394443 - Method for cloning genes - Google Patents
... reverse transcriptase from avian myeloblastosis virus, from Life Sciences, Inc., St. Petersburg, FL.; .sup.γ-32 P-ATP, of ... Cucumber mosaic virus coat protein gene. US5624805 *. May 12, 1995. Apr 29, 1997. Celtrix Pharmaceuticals, Inc.. Uses for ... Method for detecting, identifying, and quantitating organisms and viruses. US5641632 *. Jun 2, 1995. Jun 24, 1997. Gen-Probe ... Method of protecting plants by introducing a gene coded for a protein which enhances virus infection of host insects. ...http://www.google.com/patents/US4394443?dq=6,460,050
Patent US4394443 - Method for cloning genes - Google Patents
... reverse transcriptase from avian myeloblastosis virus, from Life Sciences, Inc., St. Petersburg, FL.; .sup.γ-32 P-ATP, of ... Cucumber mosaic virus coat protein gene. US5624805 *. May 12, 1995. Apr 29, 1997. Celtrix Pharmaceuticals, Inc.. Uses for ... Method for detecting, identifying, and quantitating organisms and viruses. US5641632 *. Jun 2, 1995. Jun 24, 1997. Gen-Probe ... Method of protecting plants by introducing a gene coded for a protein which enhances virus infection of host insects. ...http://www.google.com/patents/US4394443?dq=4484186
Patent US5284940 - Preparation for nucleic acid samples - Google Patents
Reverse transcription conditions were studied and optimized for obtaining cDNA from the RNA using avian myeloblastosis virus ( ... Hemin does not inhibit the activity of reverse transcriptase purified from avian myeloblastosis virus. ... Cell fusion can be induced either by virus, such as Epstein-Barr or Sendai virus, or polyethylene glycol. Polyethylene glycol ( ... The low copy situation of virus infection is a good case in point. Since the number of HIV infected T4 cells can be as low as ...http://www.google.ca/patents/US5284940
Cardiomyopathy in Transgenic myf5 Mice | Circulation Research
avian myeloblastosis virus. ANF. =. atrial natriuretic factor. bHLH. =. basic helix-loop-helix. ...http://circres.ahajournals.org/content/78/3/379
Endoplasmic reticulum stress induces PRNP prion protein gene expression in breast cancer | Breast Cancer Research | Full Text
Reverse transcription was accomplished with avian myeloblastosis virus (AMV) reverse transcriptase (Roche Diagnostics, Laval, ...https://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3398
From Gene to Protein: Information Transfer in Normal and Abnormal Cells - 1st Edition
Avian Myeloblastosis Virus (AMV) Linear Duplex DNA: In Vitro Enzymatic Synthesis and Structural Organization Analysis. Studies ... Noncoding Inserts in Hemoglobin and Simian Virus 40 Genes Are Bounded by Self-Complementary Regions. A Variant of Polyoma Virus ... Role of Protein Synthesis in the Replication of the Killer Virus of Yeast. Effects of the Catalytic Subunit of Protein Kinase ... An Endonuclease-Sensitive Region in Simian Virus 40 Chromatin. Expression of Cloned Viral and Chromosomal Plasmid-Linked DNA in ...https://www.elsevier.com/books/from-gene-to-protein-information-transfer-in-normal-and-abnormal-cells/russell/978-0-12-604450-8
Frontiers | CD25+ B-1a Cells Express Aicda | Immunology
cDNA was prepared using avian myeloblastosis virus reverse transcriptase (Bio-Rad). Gene expression was then measured by real- ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.00672/full
Protein Tyrosine Kinase and Phosphatase Expression Profiling - Cancer Research | Sigma-Aldrich
Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) (R9376), 5 U/µL.. *AMV RT buffer 5X: 250 mM Tris-HCl (T5941), 40 mM ...https://www.sigmaaldrich.com/life-science/cell-biology/cancer-research/learning-center/cancer-research-protocols/ptk-ptp-expression.html
William A. Haseltine - Wikipedia
"Specific Binding of Tryptophan Transfer RNA to Avian Myeloblastosis Virus Reverse Transcriptase". Proceedings of the National ... They proposed to use methods similar to those used to isolate the HTLV virus to find this new virus. These methods were ... "Gibbon Ape Leukemia Virus Halls Island: New Strain of Gibbon Ape Leukemia Virus". Journal of Virology. 29 (1): 395-400. PMC ... The laboratory also created hybrid viruses that carry some monkey and some HIV genes-the so-called SHIV viruses-so that new ...https://en.wikipedia.org/wiki/William_A._Haseltine
Role of Endothelin in Deterioration of Heart Failure Due to Cardiomyopathy in Hamsters | Circulation
24 Total RNA was reverse-transcribed by avian myeloblastosis virus reverse transcriptase. The gene-specific primer pair of ...http://circ.ahajournals.org/content/99/16/2171
Expression and purification of human TAT-p53 fusion protein in Pichia pastoris and its influence on HepG2 cell apoptosis.
Previous Document: Improving the thermal stability of avian myeloblastosis virus reverse transcriptase ?-subunit by sit.... ...http://www.biomedsearch.com/nih/Expression-purification-human-TAT-p53/22426841.html
- In addition to neoplasias caused in chickens by helper viruses of the avian myeloblastosis virus (AMV) complex, acute myeloblastic leukemia is induced by a defective leukemogenic component. (springer.com)
- 1980). A Hind III digest of AMV-S linear viral DNA also indicates that more than 90% of the AMV-S virus complex is MAV-1 like, while the remainder represents both the leukemogenic and MAV-2-like viruses (Souza et al. (springer.com)
- The c- myb gene is a cellular proto-oncogene from which the v- myb oncogenes expressed by two avian leukemia viruses are derived [ 12 ]. (biomedcentral.com)
- Souza LM, Baluda MA (to be published) Identification of the avian myeloblastosis virus genome. (springer.com)
- Souza LM, Komaromy MC, Baluda MA (1980) Identification of a proviral genome associated with avian myeloblastic leukemia. (springer.com)
- Souza LM, Strommer JN, Hillyard RL, Komaromy MC, Baluda MA (to be published b) Cellular sequences are present in the presumptive avian myeloblastosis virus genome. (springer.com)
- Souza L.M., Bergmann D.G., Baluda M.A. (1981) Identification of the Avian Myeloblastosis Virus Genome. (springer.com)
- Structural requirements for binding of bovine tRNATrp with avian myeloblastosis virus DNA polymerase. (uni-trier.de)