No data available that match "auxilins"


Identification of domain required for catalytic activity of auxilin in supporting clathrin uncoating by Hsc70. (1/38)

During clathrin-mediated endocytosis Hsc70, supported by the J-domain protein auxilin, uncoats clathrin-coated vesicles. Auxilin contains both a clathrin-binding domain and a J-domain that binds Hsc70, and it has been suggested that these two domains are both necessary and sufficient for auxilin activity. To test this hypothesis, we created a chimeric protein consisting of the J-domain of auxilin linked to the clathrin-binding domain of the assembly protein AP180. This chimera supported uncoating, but unlike auxilin it acted stoichiometrically rather than catalytically because, like Hsc70, it remained associated with the uncoated clathrin. This observation supports our proposal that Hsc70 chaperones uncoated clathrin by inducing formation of a stable Hsc70-clathrin-AP complex. It also shows that Hsc70 acts by dissociating individual clathrin triskelions rather than cooperatively destabilizing clathrin-coated vesicles. Because the chimera lacks the C-terminal subdomain of the auxilin clathrin-binding domain, it seemed possible that this subdomain is required for auxilin to act catalytically, and indeed its deletion caused auxilin to act stoichiometrically. In contrast, deletion of the N-terminal subdomain weakened auxilin-clathrin binding and prevented auxilin from polymerizing clathrin. Therefore the C-terminal subdomain of the clathrin-binding domain of auxilin is required for auxilin to act catalytically, whereas the N-terminal subdomain strengthens auxilin-clathrin binding.  (+info)

Molecular and functional characterization of clathrin- and AP-2-binding determinants within a disordered domain of auxilin. (2/38)

Uncoating of clathrin-coated vesicles requires the J-domain protein auxilin for targeting hsc70 to the clathrin coats and for stimulating the hsc70 ATPase activity. This results in the release of hsc70-complexed clathrin triskelia and concomitant dissociation of the coat. To understand the complex role of auxilin in uncoating and clathrin assembly in more detail, we analyzed the molecular organization of its clathrin-binding domain (amino acids 547-813). CD spectroscopy of auxilin fragments revealed that the clathrin-binding domain is almost completely disordered in solution. By systematic mapping using synthetic peptides and by site-directed mutagenesis, we identified short peptide sequences involved in clathrin heavy chain and AP-2 binding and evaluated their significance for the function of auxilin. Some of the binding determinants, including those containing sequences 674DPF and 636WDW, showed dual specificity for both clathrin and AP-2. In contrast, the two DLL motifs within the clathrin-binding domain were exclusively involved in clathrin binding. Surprisingly, they interacted not only with the N-terminal domain of the heavy chain, but also with the distal domain. Moreover, both DLL peptides proved to be essential for clathrin assembly and uncoating. In addition, we found that the motif 726NWQ is required for efficient clathrin assembly activity. Auxilin shares a number of protein-protein interaction motifs with other endocytic proteins, including AP180. We demonstrate that AP180 and auxilin compete for binding to the alpha-ear domain of AP-2. Like AP180, auxilin also directly interacts with the ear domain of beta-adaptin. On the basis of our data, we propose a refined model for the uncoating mechanism of clathrin-coated vesicles.  (+info)

Auxilin-dynamin interactions link the uncoating ATPase chaperone machinery with vesicle formation. (3/38)

The large GTPase dynamin is required for budding of clathrin-coated vesicles from the plasma membrane, after which the clathrin coat is removed by the chaperone Hsc70 and its cochaperone auxilin. Recent evidence suggests that the GTP-bound form of dynamin may recruit factors that execute the fission reaction. Here, we show that dynamin:GTP binds to Hsc70 and auxilin. We mapped two domains within auxilin that interact with dynamin, and these domains inhibit endocytosis when overexpressed in HeLa cells or when added in a permeable cell assay. The inhibition is not due to impairment of clathrin uncoating or to altered clathrin distribution in cells. Thus, in addition to its requirement for clathrin uncoating, our results show that auxilin also acts during the early steps of clathrin-coated vesicle formation. The data suggest that dynamin regulates the action of molecular chaperones in vesicle budding during endocytosis.  (+info)

Experimentally biased model structure of the Hsc70/auxilin complex: substrate transfer and interdomain structural change. (4/38)

A model structure of the Hsc70/auxilin complex has been constructed to gain insight into interprotein substrate transfer and ATP hydrolysis induced conformational changes in the multidomain Hsc70 structure. The Hsc70/auxilin system, which is a member of the Hsp70/Hsp40 chaperone system family, uncoats clathrin-coated vesicles in an ATP hydrolysis-driven process. Incorporating previous results from NMR and mutant binding studies, the auxilin J-domain was docked into the Hsc70 ATPase domain lower cleft using rigid backbone/flexible side chain molecular dynamics, and the Hsc70 substrate binding domain was docked by a similar procedure. For comparison, J-domain and substrate binding domain docking sites were obtained by the rigid-body docking programs DOT and ZDOCK, filtered and ranked by the program ClusPro, and relaxed using the same rigid backbone/flexible side chain dynamics. The substrate binding domain sites were assessed in terms of conserved surface complementarity and feasibility in the context of substrate transfer, both for auxilin and another Hsp40 protein, Hsc20. This assessment favors placement of the substrate binding domain near D152 on the ATPase domain surface adjacent to the J-domain invariant HPD segment, with the Hsc70 interdomain linker in the lower cleft. Examining Hsc70 interdomain energetics, we propose that long-range electrostatic interactions, perhaps due to a difference in the pKa values of bound ATP and ADP, could play a major role in the structural change induced by ATP hydrolysis. Interdomain electrostatic interactions also appear to play a role in stimulation of ATPase activity due to J-domain binding and substrate binding by Hsc70.  (+info)

Visualization of the binding of Hsc70 ATPase to clathrin baskets: implications for an uncoating mechanism. (5/38)

Clathrin assembly into coated pits and vesicles is promoted by accessory proteins such as auxilin and AP180, and disassembly is effected by the Hsc70 ATPase. These interactions may be mimicked in vitro by the assembly and disassembly of clathrin "baskets." The chimera C58J is a minimal construct capable of supporting both reactions; it consists of the C58 moiety of AP180, which facilitates clathrin assembly, fused with the J domain of auxilin, which recruits Hsc70 to baskets. We studied the process of disassembly by using cryo-electron microscopy to identify the initial binding site of Hsc70 on clathrin-C58J baskets at pH 6, under which conditions disassembly does not proceed further. Hsc70 interactions involve two sites: (i) its major interaction is with the sides of spars of the clathrin lattice, close to the triskelion hubs and (ii) there is another interaction at a site at the N-terminal hooks of the clathrin heavy chains, presumably via the J domain of C58J. We propose that individual triskelions may be extricated from the clathrin lattice by the concerted action of up to six Hsc70 molecules, which intercalate between clathrin leg segments, prying them apart. Three Hsc70s remain bound to the dissociated triskelion, close to its trimerization hub.  (+info)

An auxilin-like J-domain protein, JAC1, regulates phototropin-mediated chloroplast movement in Arabidopsis. (6/38)

The ambient-light conditions mediate chloroplast relocation in plant cells. Under the low-light conditions, chloroplasts accumulate in the light (accumulation response), while under the high-light conditions, they avoid the light (avoidance response). In Arabidopsis (Arabidopsis thaliana), the accumulation response is mediated by two blue-light receptors, termed phototropins (phot1 and phot2) that act redundantly, and the avoidance response is mediated by phot2 alone. A mutant, J-domain protein required for chloroplast accumulation response 1 (jac1), lacks the accumulation response under weak blue light but shows a normal avoidance response under strong blue light. In dark-adapted wild-type cells, chloroplasts accumulate on the bottom of cells. Both the jac1 and phot2 mutants are defective in this chloroplast movement in darkness. Positional cloning of JAC1 reveals that this gene encodes a J-domain protein, resembling clathrin-uncoating factor auxilin at its C terminus. The amounts of JAC1 transcripts and JAC1 proteins are not regulated by light and by phototropins. A green fluorescent protein-JAC1 fusion protein showed a similar localization pattern to green fluorescent protein alone in a transient expression assay using Arabidopsis mesophyll cells and onion (Allium cepa) epidermal cells, suggesting that the JAC1 protein may be a soluble cytosolic protein. Together, these results suggest that JAC1 is an essential component of phototropin-mediated chloroplast movement.  (+info)

Depletion of GAK/auxilin 2 inhibits receptor-mediated endocytosis and recruitment of both clathrin and clathrin adaptors. (7/38)

Cyclin G-associated kinase (GAK/auxilin 2), the ubiquitous form of the neuronal-specific protein auxilin 1, is an essential cofactor for the Hsc70-dependent uncoating of clathrin-coated vesicles. We have now investigated the effect of knocking down GAK in HeLa cells by vector-based small hairpin RNA. Functionally, depletion of GAK caused a marked decrease in internalization of both transferrin and epidermal growth factor and altered mannose 6-phosphate receptor trafficking, but had little effect on the recycling of transferrin receptor back to the plasma membrane. Structurally, depletion of GAK caused a marked reduction in perinuclear clathrin associated with the trans-Golgi network and in the number of clathrin-coated pits on the plasma membrane, and reduced clathrin exchange on the few clathrin-coated pits that remained. Surprisingly, while clathrin depletion does not prevent adaptors from assembling on the membrane, depletion of GAK caused a dramatic reduction in AP2 and epsin on the plasma membrane and AP1 and GGA at the trans-Golgi network. A similar effect was caused by expression of a dominant negative Hsp70 mutant. These results suggest that GAK, in conjunction with Hsc70, not only uncoats clathrin-coated vesicles and induces clathrin exchange on clathrin-coated pits, but also mediates binding of clathrin and adaptors to the plasma membrane and the trans-Golgi network.  (+info)

Structural basis of interdomain communication in the Hsc70 chaperone. (8/38)

Hsp70 family proteins are highly conserved chaperones involved in protein folding, degradation, targeting and translocation, and protein complex remodeling. They are comprised of an N-terminal nucleotide binding domain (NBD) and a C-terminal protein substrate binding domain (SBD). ATP binding to the NBD alters SBD conformation and substrate binding kinetics, but an understanding of the mechanism of interdomain communication has been hampered by the lack of a crystal structure of an intact chaperone. We report here the 2.6 angstroms structure of a functionally intact bovine Hsc70 (bHsc70) and a mutational analysis of the observed interdomain interface and the immediately adjacent interdomain linker. This analysis identifies interdomain interactions critical for chaperone function and supports an allosteric mechanism in which the interdomain linker invades and disrupts the interdomain interface when ATP binds.  (+info)

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Sorry gan klo :repost moga-moga bisa bikin :ngakak PREMAN A: Eh, loe tau gak kenapa preman-preman sini pada nunduk tiap liat gue? B: Gak... Gak tau bang. (sambil ketakutan) A: Karena gue pendek tau!! :ngakaks DITILANG POLISI A: Mana surat-suratmu?? B: Maaf pak, saya sekarang udah gak pake surat, saya sekarang pake e-mail A: :capedes MIMPI A: Gue mimpi dikejar-kejar anjing B: Udahlah, cuma mimpi. A
Hmm..sama laa ngan adik sy..dia ngan famili mentua dia dh booked tiket ke PADANG ujung tahun lepas lagik..Nak pi ujung November ni. Nak tak nak..terpaksa ler pi gak. Kalo tukar ke tempat lain..kena tmbah byk duit lagik ...
Chloroplasts change their position in a cell in response to environmental light conditions (Wada et al., 1993, 2003). Low-fluence rate light induces movement of chloroplasts toward the irradiated area, resulting in chloroplast accumulation at the front face of the cell (accumulation response). Conversely, under high-fluence rate light, chloroplasts move to the anticlinal wall of the cell to avoid photodamage (avoidance response; Kasahara et al., 2002). Chloroplast photorelocation movement is found in several photosynthetic plant species, including yellow and green algae, mosses, ferns, and flowering plants. In most plant species, chloroplast movement is induced by irradiation with blue light, although it is also induced by red light in some cryptogam plants (Wada et al., 1993, 2003). The flowering plant Arabidopsis (Arabidopsis thaliana) has two types of blue-light photoreceptor, cryptochromes (cry1 and cry2) and phototropins (phot1 and phot2). Cryptochrome is a flavoprotein similar to the ...
SMAP2 immunoprecipitated clathrin and AP-1 through a putative clathrin-binding domain and a CALM-binding domain, and SMAP2 mutants that did not interact with clathrin or AP-1 could not localize to recycling ...
Eh iya Mel, elu jadi di BalSud kan ya kawinannya? FYI, kemarin ini gue ke kondangan di situ, lagi2 sound systemnya kok gak enak ya. Mungkin elu kudu nambah 20rb watt lagi deh. Soalnya suara MC itu gak kedengeran, padahal MC-nya itu udah sampe teriak2 loh. Dan MC ini udah biasa di hotel dan bagus. Sayang deh kalo lu udah bayar MC mahal2, tau2 nanti gak kedengeran. ReplyDelete ...
Patot ar Ain debabbbb..sbb bapak dia pon debab gak..hehehe..bapak ain ni sbenarnye abang yan....ermmmAbg yg ketiga...tp dia anak yg ke-9 drpd 10 org adik bradik...Dia ni kawen tahun 2007...Alhamdulillah dah ade 2 org anak....yg si Ain ni anak yg ke-2...anak yg pertama lelaki....namenye... ...
Nucleotide binding to the 70 kDa heat-shock cognate protein (Hsc70) from mung bean seeds and pig brain was investigated, as well as the clathrin uncoating activity of Hsc70 in the presence of these nucleotides. The two enzymes were found to behave identically. ATP bound to two different forms of Hsc70, with dissociation constants of 1.1±0.1 µM and 1.4±0.7 mM respectively at 25 °C. This corresponds to ΔG0´ = -34 and -16 kJ/mol respectively. From the temperature-dependence of the dissociation constant of the high-affinity site, ΔH0´ was calculated to -36±2 kJ/mol. This gives ΔS0´ = 6.7 J/mol per K. Adenosine 5´-[γ-thio]triphosphate, ADP, adenosine 5´-[β,γ-imino]triphosphate and adenosine 5´-[β,γ-methylene]triphosphate showed dissociation constants of 2.3, 11, 31 and 284 µM respectively. The order of affinities corresponded to the order of effectiveness in uncoating of pig brain coated vesicles. The implications of these findings for the mechanism of Hsc70 action are discussed. ...
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Udah lama gak update blog, mestinya nulis tentang update sekolah Anelka yaa hehe.. *kumpulin niat* Anw gak terasa banget duedatenya Mimi semakin dekat.. Salah satu yang mau dibenahi menyambut kelahiran itu mobil. Karena mobil yang ada cuma 2 row, dan kalo ditambah 1 carseat lagi jadi sumpek pek gak muat apa2 lagi.. Mana lagi, sampai…
alhamdulillah....harap sumenye okay sentiasa..eida jgn lupe doakan utk nis key..nis pn sentiasa doakan baby eida pn okay gak..yeke baby girl??he..he..tapi nape ramai yg ramal nis bakal dpt boy lagik??kalender cina pn camtu gak..kire suprise nih..!! ...
all of a sudden the older one went in his litter box and peed a little, then jumped on a sofa in our sun room and peed a little. (Thank goodness we have a blanket/sheet on all sofas so we dont have too much fur on them. He then went on the sofa in the Living Room and did the same ...
Researchers will formulate a model that predicts the spatiotemporal variability in groundwater vulnerability to UO&G-associated chemicals. This statistical model will be trained with calculations of a physically based, hydrologic model that simulates the probability of overlap between capture zones of drinking-water wells and UO&G contaminant sources in Susquehanna County, PA and Belmont County, OH. Once calibrated, the resultant vulnerability index model will be tested against water-quality data collected in a large-scale sampling campaign of 500 homes across four additional PA and OH counties. Water samples will be analyzed for a broad range of inorganic and organic compounds, including UO&G-related contaminants that cannot be detected by conventional analytical techniques. Our studies will culminate with a case-control study of 8,000 neonates in PA and OH to determine whether exposure to UO&G contaminants (as estimated by our vulnerability index model) is associated with changes in the ...
Specific receptor-mediated endocytosis pathways can be investigated with fluorescent ligands including pHrodo™ dyes targeted to common receptors, including, EGF, LDL, and transferrin.. The most convenient, no-wash assay format uses pHrodo™ Red or pHrodo™ Green to allow discrimination of stages in the endocytic pathway from early endosome to lysosome formation with no quench or wash required.. pHrodo™ dyes are essentially non-fluorescent at neutral pH and exhibit increasing signal with a red or green readout respectively as the pH decreases. The increase in fluorescent signal can be used to monitor progression in the endocytic pathway.. ...
gimana sih caranya bikin web yang keren gini, ajarin dunk mas..:D. kunjungi web saya juga mas…kasih coment, biar bisa lebih maju dalam nge bLOG..huehue…paling engga sesama rekan kerja bisa bagi bagi ilmu, meskipun berbeda pulau mas..hehehe... oh ya..belum kenal udah sok akrab, tapi gak apa kan mas..dari pada sok musuhan..hehehe..sekali lagi..salam kenal mas dari RuL di ujung pulau sumatera di Bumi Serambi Mekkah. keep update mas…:). ...
:selamat SELAMAT DATANG :selamat BUAT AGAN/AGANWATI YANG BAIK GAK BAKALAN LUPA SAMA KOMENGNYA SERTA :rate5 DAN :cendolbig Semoga Gak :repost Indonesia negara tercinta kita ini berada dalam cicin api pasifik, atau kata mudahnya memiliki banyak gunung berapi, gunung berapi yang dianugrahkan di negara kita memang memiliki 2 sisi, yaitu anugrah karena bisa menyuburkan tanah:malus, serta bencana jika k
30 Ogos plak bday girl... Sape lg yg gegirl dlm umah ni kan... Ofkos la mama...hahaha... Tp yg geram + nk merajuk tp xjd, maghrib ms buka puasa tu bru la Abg igt nk wish..huhuhu sabo je la kan... Seb baik ada alasan yg kukuh...xpe la, dimaafkan... Utk bday girl plak, msk sendiri gak... Wat roti jala ngan kuzi ayam... Roti jala tu pki tepung blue key...hahaha punya la xcek brg dapur, rupanye tepung gandum abis dah.. Hentam je la... Sdp gak tau ...
Sering mengeluh soal barang bawaan dalam tas sangat berat? Toss dulu sama saya! Gak tau kenapa selalu bertahan untuk bawa tas yang berat ini. Apa karena pa
Recent mooring observations of ocean temperature provide the first high resolution, long term record of temperature variability in the Makassar Strait of the Indonesian Seas. The mooring observations span the entire cycle of the strong 1997/1998 El Nino. A high correlation (r = 0.67) is found between variability in the average thermocline temperature, to variability in the southward Makassar volume transport: during high (low) volume transport, the average temperature of the thermocline is also high(low). In addition, from nearly 15 years of XBT data, the Makassar thermocline temperature is shown to be highly correlated (r = 0.77) to SOI. This reveals that the Makassar temperature field - when coupled with the throughflow transmits the equatorial Pacific Fl Nino and La Nina temperature fluctuations into the Indian Ocean. The ENSO variability in the internal energy transport is calculated: 0.63 PW during the La Nina months of December 1996 through February 1997, and 0.39 PW during the El Nino ...
PhD Project - High resolution cryo-electron microscopy of clathrin cage networks at University of Warwick, listed on FindAPhD.com
Characterization of clathrin-coated vesicles isolated from MPR-deficient fibroblasts. The material contained in fractions 8 to 10 of the density gradients sho
dan dia juga yg tolong aku balut..serius..dia yg tolong pegangkan kertas pembalut ni bila aku sibuk gunting seletep....hehehehe....senang..dah tak payah suh laki aku tolong aku ...
1st ni hakak nak ucapkan ribuan terima kasih pada Astro & Redmummy yang sudi panggil hakak sebagai jemputan tuk Project Alpha tempoh hari(korang dah undi tuk vote kak red)...Bukan hakak sorang jer yer dek non..kak rose, sid pun ada gak..Tapi kak ayu ngan yanok x dak...x per janji tempias2 tu tetap ada kan...GBM tetap kitorang bawak jugak..kan..Apsal hakak letak letak Rimmel punya make-up?Dan-dan jer tau...Coz, hakak baru dapat from sid..Walaupun kak red dah lama bagi kat hakak..tapi hakak x sempat nk jumpa dorang...Last2 sid gak tolong hantarkan kat offc hakak ...
Eunhyuk itu pelit. kalau pergi bareng dan masalah bayar - membayar dia suka bilang sama Leeteuk " Hyung, bukannya kamu yang bayar?". Kata Leeteuk, Eunhyuk suka ngutang tapi lupa bayar. Ini juga yang dikatakan sama Sungmin (kalo gw gak salah ingat! Ingatan gw rada2 cuy… hehehehe) pas di Intimate Note! Dia ampe teriak, aku ini dongsaeng mu tapi kau gak pernah bayarin aku makan! Hahahaha… ...
Buy Korg NanoPad 2 (White) Slim-Line USB Pad Controller online from GAK.co.uk. Unbeatable prices and next day delivery from the UKs no1 instrument store. Order Today.
Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinsons disease, on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parsons lab at Kings College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinsons. Read more on her story here. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 23rd Feburary 2018. Apply now!. ...
Expression of GAK (DNAJC26) in adrenal gland tissue. Antibody staining with HPA027405, HPA027459 and HPA027463 in immunohistochemistry.
camtu la gak asyraaf time kat spital aritu... kesian tol.. rase cam nk menitik air mata time dia kena amik darah.. 3 kali cucuk br kuar darah... makan pn x lalu.. aku wat bubur pn dia x mkn.. last2 aku blend... br dia mkn.. mungkin time tu xde selera nk mkn kan. risau btl bile selera anak hilang.. macam2 vitamin la aku bg... gelabah gak kan kalu brdua ngan asben je time2 gni.. time ni gak la rase cam nk je mak ayah kita ade skali.. :D ...
p/s: tinggal 3 hari lagi nak final sem 2. kadang2 auni rasa blur bile tengok soklan past year. especially part anatomy. Auni tak tau orang lain macammane. tengok macam dorang sume dah ready. but semalam ade yang cerita kat auni menangis2 sebab stress sangat dengan exam ni. buat yang berkenaan, tak usah pikir2 lagi. sbenarnya jawapan tu sume da ade lam kepala kita. cuma kite terlupa mane kite letak kunci for jawapan tu. macammane nak dapatkan kunci tu balik? senang. RELAX..tarik nafas. when u feel stress, switch to thing which make u NOT stress. macam tgok citer industan ke (like i did yesterday), nyanyi ke (like i did yesterday), menari ke (like i did yesterday), berangan ke (like i did yesterday) or masak2 (like i did yesterday..err.. asal rase cam banyak semacam je auni buat semalam? em. aah.betol.. mmg banyakla). after that ull find the way.trust me. U WILL. k? otak pun nak relax gak. jangan pakse. otak tu makhluk tuhan gak. mesti die ade perasaan.kan? kan? (terbayang otak itu macam kartun ...
dan balik umah td,mak abah kecoh kangah dpt offer letter drpd poli..kangah yg spatutnye masuk ntok intake november telah dinaiktaraf ntok intake jun..klaw die trime offer tuh, kenalaa mendaftar next week! tekejut kot.. tp kalaw aq jadi die sah2 dah grab dah, sbb memula mmg x nmpk tp bile org sekeliling da grad,da keje..haii, x sabar nk grad cpat2 dan pgg duit sndrik..kire mmg rezeki die dpt offer gitu, jarang kot terjadi dan satu ag menurut pendapat org ramai, method poli ni sgt beza ngn universiti, sbb poli utamakan kemahiran dan perlu bersaing lebih dgn graduan universiti,jadi watpe nk buang masa satu sem kan.. tp tepulanglah kat tuan punya badan kan..susah gak nk get used sbb x penah dok asrama..dan skali dok asrama terus kena campak jauh..dilema gak tuh.. sdgkn aq pun x pernah dok luar selangor,tu yg teruja tuh! tp nilah dipanggil perubahan fasa kehidupan..dr kecik ke besar,dari murid skolah ke mahasiswa dan dari rumah ke hostel ...
Sampai sana ahad kul 1 ptg.. Sampai2 sana je terus makan dulu.. Memang sedang elok jer waktu lunch kan.. Hujan... Sempat gak kami bakar ayam dan daging kambing.. Pastu kami cari tempat tido.. Sebelum tu ade call 2 chalet.. dua2 penuh.. Mase sampai sane tu mmg tawakal je la cari chalet.. Hampir 2 jam cari dan tanye hampir 20 chalet, akhirnya dapat gak... huhu.. La Bella Resort... Ngam2 je yg kosong adalah sebuah dorm utk 14 orang.. Alhamdulillah memang rezeki kami la ...
Lanjut cerita soal shopping-shopping…Kemarin waktu pulang ke Semarang, aq sempet shopping-shopping…hehehehe…Cuma karena buru2, akhirnya barang2nya gak kefoto deh…zzzzz….Ternyata ibuku udah beliin kebutuhan-kebutuhan baju baby yang dasar…Jadi aq udah gak beli-beli lagi deh…Baju-baju lengan panjang, lengan pendek, tanpa lengan, popok kain, celana pendek, celana panjang, crelana pop, popok kain, gurita, alas ompol, dll udah dibeliin semua sama ibuku…Tadinya pengen beli yang merk Fluffy, Libby ato Nova…menurut referensi merk-merk itu yang banyak direkomendasiin…Ternyata udah dibeliin ibuku…ibuku beli yang lusinan, lebih murah jadinya…kainnya juga lumayan kok…Makasiiiihhh uti-nya dedek… =) Kemarin di Semarang akhirnya beli keperluan baby selain baju-baju basic…Aq belanja di Sejati baby shop Jl. Sompok…Rameeee deh disana…banyaak banget yang beli…tempatnya cukup nyaman…beda sama Kitty, klo di Kitty kan qt bisa ambil-ambil n pilih-pilih sendiri..Kalo di Sejati, qt ...
Tiba-tiba kepikiran soal ini : kamu gak perlu nikah untuk tahu dia pasanganmu yang sah atau bukan. Kamu cuma perlu seks. Haha. Maybe not. Kamu cukup tahu bahwa kamu seneng dia pasanganmu, kamu seneng mantanmu cuma sekedar mantan, syukur-syukur masih friendly as close as a sodara to you.
Gw dibeliin mie ini sama nyokap sebelum booming, tanpa tahu kalo nih mie pedes banget, kirain mie goreng Korea pake gochujang biasa, kan kayanya enak tuh ya... Pas makan bujug dah *gw ga tahan pedes*. Kedua kali masak, bumbunya gak dipake semua, plus dicampur indomie goreng, lalu dibagi dua (makan berdua Benny), Tetep aja looooh gw ga tahaaan. Padahal rasanya enak banget menurut gw, mie nya kenyal2, tapi kenapa musti ekstrem gitu sih pedesnya????? Btw Benny menurut standar gw doyan cabe tapi dia kepedesan juga. Ckck. ReplyDelete ...
feeling much better. semalam akhirnya gue memutuskan untuk makan obat lodia dan minum obat maag plantacid buat ngilangin nyeri. lumayan hari ini enakan perut. nyeri berkurang dan ke belakang gak sesering tiga hari lalu yang mana kayak dikuras habis semua isi perut. perjuangan banget dah ...
Sunday, March 29, 2015, 3:55 PM - 6:10 PM • Room 258C. Follow the conversation: #protein. Chaired: E. Rhoades. 3:55 Chairs Introduction.. 4:00 109.1 Mechanisms of Folding upon Binding J. Clarke. Univ. of Cambridge.. 4:25 Changes in Water Availability or Molecular Crowding Induce Folding in Instrinsically Disordered Stress Proteins from Plants A.A. Covarrubias, C.L. Cuevas-Velazquez, L.Y. Rivera-Najera, D.F. Rendon-Luna, G. Saab-Rincón, M.A. Martinez-Yamout, H. Dyson and C. Amero. Biotechnol. Inst., UNAM, Cuernavaca, The Scripps Res. Inst. and UAEM, Autonomous Univ. of Mexico State, Cuernavaca. (712.11). 4:40 109.2 The Role of Protein Disorder and Self-Association in the Formation of Cellular Bodies T. Mittag, M.R. Marzahn, J. Lee, A. Palud, S. Marada, A. Nourse, J.P. Taylor and S.K. Ogden. St. Jude Childrens Res. Hosp.. 5:05 Specificity of the Yeast Auxilin Homolog Swa2 in [URE3] Prion Propagation Is Solely Determined by the TPR Domain J.K. Hines, E.M. Troisi, M.E. Rockman and E.E. Oliver. ...
hai dila..sy tulun jwbkn la ea..hihi..aritu sy de p cari brg2 cross stitch..lau nk cari benang n brg2 die tu cube jln ke depan sket dr semua house tu..cari cimb n maybank..ade kedai barang alat2 jahitan n brang2 hntaran sebelah je ngan cimb tu..kdai tu agak lengkap la ..benang pon bnyk pilihan..cuma dr segi harga tu standard RM1.80..ade lg satu kedai jual brg2 hantaran n bunga2 menghala ke masjid india yg kaler oren tu pon de jual gak brg2 cross stitch n benang..cuma dr segi hrga kain tu, kt kdai ni lebih mahal..tp hrga benang sama je..ha kt level bwh semua house tu pon ade gak..benang dmc cume rm1.20 je tp xbnyk pilihan gat..n brg2 len cam kain pon xde dijual..dlm ketiga-tiga kedai nih, cuma yg kedai menghala ke masjid oren tu je ade jual pattern..tp tu pon pattern lama..cube cari pattern online..bnyk gak yg latest n lawa..hope info nih membantu..hihi..^___^)v~. ReplyDelete ...
Sbb dia banyak masa terluang dia tolong Aso kt Biology Club.. Sejak tu la dia rapat. Aso ni pn sedih gak sbb kehilangan abang dia. Abang dia lemas masa dorg sama² memancing. Ayah Aso seorg doktor & dia nk Aso jd doktor gak. Tp Aso ni jenis keras kepala, x macam abg dia. Bila Aso kawan ngan Aya, ayah Aso x setuju sbb dia rase Aso ni terlalu muda & x matang. Die xnak nnt, bila saat Aso da bosan jaga Aya (sbb keadaan dia meneruk), Aso tinggalkan Aya, pasti yg rasa terluka tu nnt Aya sdr. Tapi Aso x peduli sbb dia mmg kawan yg baik... Sebab Aya, Aso pilih nk jadi doktor w/pn die masih rasa usaha doktor tu semua sia². Sbb xde doktor berjaya ubati Aya pn.. Aso la kawan yg sentiasa ade di sisi Aya. ...
Sbb dia banyak masa terluang dia tolong Aso kt Biology Club.. Sejak tu la dia rapat. Aso ni pn sedih gak sbb kehilangan abang dia. Abang dia lemas masa dorg sama² memancing. Ayah Aso seorg doktor & dia nk Aso jd doktor gak. Tp Aso ni jenis keras kepala, x macam abg dia. Bila Aso kawan ngan Aya, ayah Aso x setuju sbb dia rase Aso ni terlalu muda & x matang. Die xnak nnt, bila saat Aso da bosan jaga Aya (sbb keadaan dia meneruk), Aso tinggalkan Aya, pasti yg rasa terluka tu nnt Aya sdr. Tapi Aso x peduli sbb dia mmg kawan yg baik... Sebab Aya, Aso pilih nk jadi doktor w/pn die masih rasa usaha doktor tu semua sia². Sbb xde doktor berjaya ubati Aya pn.. Aso la kawan yg sentiasa ade di sisi Aya. ...
Seperti judulnya, postingan kali ini gak terlalu serius, enteng-enteng aja. Anggap kita sudah seminggu ini belajar dan berusaha keras, dan sekarang di akhir
wa rasa sbb dia tau profile dia ada byk problem,byk kes,tu yg dia kena cepat tabur gula2 kat tanah.satu cara yg cepat,dgn ikut idea2 anwar.sume dia nak buat beb.1st dulu,idea lompat2 parti.pastu,16sept dah umum cuti penubuhan msia.skrg pun dah pandai guna perkataan rakyat.sekolah 1 aliran dia nak buat gak ...
ups....aebelum aku sign out.. lupa plak yg aku ada byk koleksi gambar dania bobo... sukerrr sgt menatap wajah indah tatkala dania bobo... (ekceli, masa pegnen dulu aku shuuuukeeeeeeeee sgt tgk muka hubby gak time hubby tido...hikhikhik...) ...
Natúrkozmetikumok az ápolt, fiatalos, egészséges bőrért, a lélek szépségéért. Kaviczky természetes, vegán arc- szemkörnyék- és testápolók virágkivonatokkal, drágakövekkel és a gyógyvíz erejével. Tanácsadással segítjük, hogy milyen krémet válasszon.
And no, its not just soup, hot tea, and warm sammies. These foods are so incredibly surprising but are all backed by scientific data.
Internalization of cargo proteins and lipids at the cell surface occurs in both a constitutive and signal-regulated manner through clathrin-mediated and other endocytic pathways. Clathrin-coated vesicle formation is a principal uptake route in response to signalling events. Protein-lipid and protein-protein interactions control both the targeting of signalling molecules and their binding partners to membrane compartments and the assembly of clathrin coats. An emerging aspect of membrane trafficking research is now addressing how signalling cascades and vesicle coat assembly and subsequently disassembly are integrated.
Gentaur molecular products has all kinds of products like :search , Exbio \ Clathrin heavy chain \ 11-526-C025 for more molecular products just contact us
Sori sayang...mama lewat wat entri spesial nie... Bad faham2la akhir2 nie mama sering x sht...kadang2 sakit sana..sini.. macam ari nie MC lg...huhuhu...tp ekceli..daku ingat nak g keje gak pagi tadi...bangun tido kaki krem..gagah gak g mandi..then kaki macam x ley pijak sakit kat bawah pelipat dan batang betis..daku baring jap..ingat dlm kul 10pg tu nak g la...tiba2 dengan muka minta kesian..Bad tanya.."mama nak g keje gak ke? Ari ni kan besday Abg Bad..sampai hati...la .... dia request perhatianku rupanya....daku gtau..mama da tempah kek kat kawan sayang...nanti mama balik bw kek ye...hadiah kan da dapat ari tu..Beskal yg da beberapa kali Bad jatuh tuh"...alamak..dia start konsert air mata lak...huhuhu..last2 daku x sampai hati..daku sms bos daku gtau x sihat ..nak g klinik..da mmg x sht so mmg dpt mc la..bonusnya dapat 2 hari sbb doktor tengok tangan n kakiku bengkak x surut2...alhamdulillah... balik dr klinik (mak bw kete..daku x mampu ..) terus g amik kek kat opis disamping hantar MC tuh... ...
Memang bener kata2 orang bijak, orang2 soleh, orang2 pintar, orang2 cerdas, orang aring dsb, bahwa memang petuah dan nasehat itu bisa datang dari mana saja. Terkadang nasehat itu lebih kueren dari pada petuah2 orang bijak, motivator, eterpreneur dan semacamnya.. Berawal dari kelaparan setengah mati, akhirnya memutuskan mampir di lontong pecel "Peni" Sirojudin sekaligus kangen2an lama gak mam situ. Asik nih kalo mam situ, berasa kek anaknya sendiri, minta pecel model apa aja pasti dikasi, ples diajak ngobrol pula.. Singkat kata singkat cerita..berawal dari obrolan kaki ne mbak.e sek jare segedhe gaban, celana gak muat, pengen kurus, sampe cerita KrisDayanti yg udah beranak 3 tp msh tetep langsing…disambung cerita kehilangan uang…kehilangan motor dimana sang maling tu sempet ngajak senyum ma ibunya..sampe maling dibakar,..digebugin..anakn. ...
A blog about an Advanced Dungeons and Dragons (AD&D) First Edition or OSRIC compatible megadungeon. Get a free dungeon room every day.
Last nite, entah nape la tetibe rase groggy. Huhu..pelik betul xde symptom2 awal, tau2 je demam panas. Patutla tak de selera makan 2-3 hari ni. Dahla demam panas pastu plak kene diarrhea teruk. Pkul 5 pagi dh bgun pegi cari toilet. Bukan sekali pegi tp berkali-kali gak la. Pagi tu, g klinik UMK time tu la plak doktor xde..kne suruh g pkul 10pg. Aishhh ...
Ambillah kesempatan cuti sekolah ini untuk membawa anak-anak anda bercuti. Masih ada 2 hari lagi untuk anda ke sana. InsyaAllah anda tak akan rugi ke sana kerana terdapat banyak aktiviti sampingan yang dijalankan. Anda juga berpeluang naik belon udara panas tersebut dengan bayaran yang telah ditentukan. Teringin gak nak naik belon tu. InsyaAllah jika esok ada kelapangan saya juga akan ke sana. ...
Ini J-pop sudah lama nongkrong di Kompi.... yah sejak 2 tahun lalu pas dihibahin bejibun J-pop sama mbak Rin. Kebetulan baru-baru ini dapat dorama hanakimi juga dari si mbak. Eh gak tau nya Ikenai Taiyou ini salah satu OST dorama tersebut. Yah, langsung suka lagi deh...hehCekidot Lyricnya...Ikenai taiyou Na NaChotto de ii kara misete kurenai…
Kenalilah dirimu maka kamu bisa mengenali dunia, bahagiakanlah dirimu maka dunia akan tersenyum bersamamu. Iseng - iseng baca sebuah artikel di tautan FB terkait masalah MKD bobrok yang sekarang ini lagi booming, eh tau - tau bisa dapet artikel yang ngebahas tentang anak no 2. Sebenarnya gak percaya sih sama kaya gituan, itu kan seperti…
Marburg virus life cycle begins with virion attachment to specific cell-surface receptors, followed by fusion of the virion envelope with cellular membranes and the concomitant release of the virus nucleocapsid into the cytosol. The virus RdRp partially uncoats the nucleocapsid and transcribes the genes into positive-stranded mRNAs, which are then translated into structural and nonstructural proteins. Marburgvirus L binds to a single promoter located at the 3 end of the genome. Transcription either terminates after a gene or continues to the next gene downstream. This means that genes close to the 3 end of the genome are transcribed in the greatest abundance, whereas those toward the 5 end are least likely to be transcribed. The gene order is therefore a simple but effective form of transcriptional regulation. The most abundant protein produced is the nucleoprotein, whose concentration in the cell determines when L switches from gene transcription to genome replication. Replication results in ...
Dari bbrp hr setelah masa subur lewat, dada sy (baca : payudara) kerasa nyeri n membengkak. Pikir sy ini tanda2 PMS, tp sy jg smpet heran koq sedini itu gejala PMSnya, msh kurang 10hari lebih dr jadwal haid. Akhirnya sy abaikan aja n lanjut aktivitas sehari2 kayak biasa, masak, cuci setrika, bersih2 rumah, urus toko, jalan kaki ke pasar, dll. Trus hari jumat tgl 25 Maret, sy ngerasa tenggorokan sy gatal bgt kyk mau batuk. Sy cm makanin permen mentol gt aja sambil minum jeruk nipis. Trus akhirnya bener hari minggunya sy kena batuk. Minggu mlm sy mulai ngerasa badan sy gak enaaak bgt, kyk mau demam or masuk angin, sakit semua. Sy pikir mmg begitu kan, batuk di hari2 awal mmg bikin rontok badan, ditambah sy ngerasa PMS jg, tambah maknyuss rontok badannya. Sebelumnya, minggu sore itu sy blg ke hubby klo mau beli obat batuk, biar batuknya gak keterusan n lama sembuhnya. Tp hubby gak ngebolehin sy mnum obat, kyk dia feeling gt kalo nanti jadi gmn. Sy sempet ngeyel wkt itu, sy blg sm hubby "apa2 ...
aku mengharap....dpt wat loan ngn in law aku....huh...ni seme over budget utk renovate umah...harap2 lar dpt....takut gak sebenarnya....klau tak dpt pinjam gak kene cari alternative lain lar ...
haa dr celcom colony yang menari2 di bloh contest..alhamdulillah ada gak BE baru..sudi gak nuffnang bagilu BE.so ada yang 2 ketul lama punya n yang baru dr celcom..n heartbeat tak tampal semula sbb takde broadband kat umah sejak maira ilangkan benda tuh n tak on2 sampai skang.n opis plak takleh nak buakak heartbeat. ...
Ini adalah salah satu bedak tabur paling populer sejak jaman dulu kala ya. Tapi menurut saya, setelah mencoba berbagai merk bedak tabur lokal (hehe gak pernah beli bedak tabur merk luar), yang harganya kadang lebih mahal daru Marcks ini pun so-so aja kualitasnya. Beberapa bedak tabur yang saya coba sih antara lain Viva (bedak Keluarga namanya, kemasannya mirip Marcks), Wardah Luminous, Marcks Venus (yang lebih mahal harganya). Karena pemakaiannnya irit, jadi saya jarang repurchase sehingga varian yang saya coba baru itu-itu aja. Mana dulu saya gak ngeh fungsi bedak tabur sehingga saya cuma ngandalin bedak Two Way Cake doang, kacau ih ...
Well, andai sobat gibol tinggal di Brazil ternyata bisa loh nonton live piala dunia dalam ponsel Nokia. Yep, soalnya Nokia Lumia 630 edisi spesial Piala Dunia ini membawa fitur baru yaitu mampu menerima siaran TV Digital berstandar ISDB-T. Standar ISDB-T memang udah jadi standar siaran televisi di Brazil sob jadi orang Brazil bisa nonton gratis. Nah karena di Indonesia masih pake standar DVB-T2 yah kita gak bisa pake fitur ini dulu yah sob. # ...
Buy Zoom SGH-6 Shotgun Mic online from GAK.co.uk. Unbeatable prices and next day delivery from the UKs no1 instrument store. Order Today.
org panggil aku is. ade yg panggil afiq. ade gak yg pnggil is afiq. skarang study kat Faculty of Medicine,Ain Shams University,Cairo,Egypt. berharap dpt menjadi doctor yg hebat ...
Temen pasti ada yang lama dan pasti ada juga yang baru.. seperti di sini, saya ketemu banyak temen2 baru, sobat blogger yang baik-baik, lucu-lucu, dan imut-imut... *saya anggap imut semua, gak tau yang gede, kecil, ato amit...semua dianggep imut... :) :zNah, kemaren hari Sabtu malem 28 Februari 2009 saya ketemu dengan temen2 lama, sebenernya saya…
Dah terkeluar dari topik plak.. gini je.. kepada perokok tegar.. sempena hari tanpa tembakau yang diadakan setiap tahun pada 31 Julai... apa kata korang hormat kat orang-orang yang tak merokok.. sehari je bro... cuba test korang boleh tak hidup tanpa rokok??? Ala nanti korang kena stop smoking gak di siang hari pada bulan puasa... ke tak puasa?.. Fakta yang belum pasti erk.. ramai lelaki yang merokok, kalau tak makan tu no hal.. tapi kalau tak merokok... pusing satu kepala.. last-last ponteng puasa... alang-alang dah ponteng makan je la sekali... hahahhaa ...
Malam kelmarin mak bawak anak mak ni g klinik..Nak tahu kenapa?? hmm, taik anak mak ni keras..tiap kali nak berak, tiap kali tu jugakla deir menangis..kesian..pastu, g la bwk dier ke klinik...tgk la aktifnya dier...siap ketuk2 pintu bilik doctor lagi..ahahha...bila msuk dlm, doctor masukkan minyak ikut lubang bontot dier (mmg khas ntuk bby yg alami mslh ni la..) ..dan2 tu gak melabur uolsss... kesian hazreeq... ...
The E-appliance is an easy to use diagnostic tool. When a patient wears the E-appliance for a single night, the absence of pain in the morning indicates a muscular problem that can be predictably treated. Pain that persists or increases indicates an internal derangement, which requires a different treatment. A single arch appliance will allow you to make a proper diagnosis only if it is properly balanced and the joint is in its most natural position ...
The history of the universe is a history of entropy liberation, which means, simply, that entropy increases. But what does that mean? Many sources will tell you that the increase of entropy is dissipation[1], degradation, and dissolution, and theyre right, but theyre not entirely right. It is a glass half empty/full sort of situation, for along with the dissipation, degradation, and dissolution comes everything else. ...
Aku dah melintasi satu bilik. Lek je. Aku wat muka slumber badak. Badak pun mau mati pengsan tengok aku wat muka cam dia. Sampai bilik kedua dari bilik aku.. Tiba-tiba mata aku melirik pada benda hitam kat longkang. Zupp! Sekali.. Aku tak tegur. Rilek lagi. Zuupp!! Kali kedua. Aku tak toleh lagi. Muka masih badak lagi. (badak jantan ok bukan badak betina). Zupp! Kali ketiga weh!! Aku dah tak tahan dah kali ni. Aku nak pandang gak biarlah apa je yang melintas-lintas tu. Matlamat aku nak tengok gak. Aku pun berani tak berani (muka badak da tuka jadi muka beruk nervous) toleh la kat benda yang zupp! Zupp! tadi tuh.. ...
after breakfast while blogging, playing guitar and reading the bible -- johanna went out of her room to paint the scenery. i missed painting, better go and start now. btw, kok gw blum dapet2 ya...? GW GAK HAMIL! Nih anak, cowok aja gak punya mau hamil ...
Kalo di Jakarta ada Soulmate yah..bisa pesen pake Palladium..Nah di Surabaya???Berhubung aq n bebekq sama2 perantauan jadi gak tau tempat2 yang jual perhiasan custom gitu..Setelah tanya2 ke temen kantorku, dimana nyari cincin yang bisa pasangan tapi yang cincin cowoknya bukan dari emas, dia kasih rekomendasi toko...dan tokonya toko online sodara-sodara...Waduuuhh...gimana ini??bebekq sempet ragu2 gitu..Tapi temenku bilang kalo toko ini reccomended...Udah ada beberapa temenku yang pesen di toko ini dan hasilnya gak mengecewakan...Nama tokonya Loving You Wedding ring...Setelah bebekq yakin, baru deh nyoba tanya2 ke ownernya..Ownernya namanya mbak Anik, helpfull banget kok mbak Anik ini...Ternyata dia juga terima custom order..Langsung deh pengen cincin yang modelnya lain dari yang udah pernah kita liat...pengen yang unik dan beda gituuu..hehehehe...Aq n bebekq berkreasi bikin cincin dengan penggabungan dua model cincin..Setelah dijelasin dengan gambar lewat email n mbak Anik ngerti soal modelnya, ...
Hari raya ke-5 Ash 1 family dtg beraya kat umah parents aku di JB..huii..ramai gak aa..family dorang je adala 20++..huhu..dorang g bercuti kat Tioman *ape daa..org sibuk beraya dorg bercuti..rugi x de kampung..huhu* then balik from Tioman, dorang sume tros ke JB.. 4-5 kereta gak la dorg tuh..kitorg 1 family pon bertungkus lumus masak & my dad selaku Chef Besar di umah kami yg masak & mengeluarkan sgala resepi di kpala..kami yg pompuan 3 org tulung tgk & prepare benda2 lain..haha ...
penat dowh balik dari perak....duduk dalam keta 9 jam ari ni dan semalam... aku gak yang gatal2 nak ikut...sebab borink duk hostel x der mende nak buat...x der ke???? padahal keje bertimbun...repot 2 bijik...campur esemen bi lagi....pretest 2 bijik nunggu isnin ni....tapi x pelar asalkan aku bahagia... study nanti boleh buat.... ahahahahahahha ...
para mapres ini malah pada berantem. Emil salim gak usah lah jadi presiden jom,udah lewat masanya.Biar dia dan habibie jadi inspirator yang muda, sering sering masuk talkshow,buat nyaingin yang ngomongnya komprador dan komprador lagi itu, Biar seimbang ide ide yang berkembang. Orang kayak emil salim itu hebat ya, lahir di jaman jepang ama belanda tapi pikirannya jauh ke depan. Berkembang terus. Dunia udah arahnya ke kooperasi global, kok masih mikirnya jaman penjajahan, komprador dan kooptasi ...
yg majalah lain pernah ter-beli macam Mami&Baby (agak serius, a bit boring la for me..)dan satu tu, apa nama tah Maternity ke MAternal magazine. pun bosan gak.. hihi... ...
Hayo siapa yang sering berkegiatan outdoor atau kulit sering merah dan gatal karena kepanasan sinar matahari? Nah.. gak perlu kuatir lagi, kini ada aloe vera mist dari innisfree ini, yang bisa menenangkan kulitmu yang terbakar sinar UV ...
Grandes Tueuses: M\u00e9ningite - l\u0027histoire (FR)","M2":"Description:","[Truncate(150):Document.CaptionLong]":"Dans l\u2019histoire de la m\u00e9decine, la m\u00e9ningite est totalement absente des descriptions antiques. Pas un mot chez Hippocrate, le p\u00e8re grec de la m\u00e9decine...","GAK":"[email protected]**","EID":"24BDHUHDSB05","IID":"MSF171077 ...
Long Ago... I know history implies that well go far back into the familys past, but the truth is that I dont know all that much about the history of the family before my parents met. Here is about all I know. On Dads side, his mother was Lillian McSpadden. She had two siblings --…

Reactome | Clathrin recruits auxilins to the clathrin-coated vesicleReactome | Clathrin recruits auxilins to the clathrin-coated vesicle

Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
more infohttp://www.reactome.org/content/detail/R-RNO-8868659

Reactome | Clathrin recruits auxilins to the clathrin-coated vesicleReactome | Clathrin recruits auxilins to the clathrin-coated vesicle

Clathrin recruits auxilins to the clathrin-coated vesicle (Bos taurus) Clathrin recruits auxilins to the clathrin-coated ... Clathrin recruits auxilins to the clathrin-coated vesicle (Canis familiaris) Clathrin recruits auxilins to the clathrin-coated ... Clathrin recruits auxilins to the clathrin-coated vesicle (Gallus gallus) Clathrin recruits auxilins to the clathrin-coated ... Clathrin recruits auxilins to the clathrin-coated vesicle (Sus scrofa) Clathrin recruits auxilins to the clathrin-coated ...
more infohttp://www.reactome.org/content/detail/R-HSA-8868659

Auxilin depletion causes self-assembly of clathrin into membraneless cages in vivo.Auxilin depletion causes self-assembly of clathrin into membraneless cages in vivo.

Both auxilins bind clathrin, adaptors (5?8) and dynamin (9). Their C-terminal J domain confers the specificity for binding to ... Relative abundance of auxilins To estimate the relative abundance of auxilin 1 and auxilin 2 in HeLa CCVs, we used our ... Depletion of both auxilins in HeLa cells resulted in a change in cell morphology with most cells appearing more rounded, which ... Auxilins / genetics, physiology*. Cell Membrane / metabolism*. Clathrin / chemistry*, metabolism*. Clathrin-Coated Vesicles / ...
more infohttp://www.biomedsearch.com/nih/Auxilin-depletion-causes-self-assembly/18489706.html

Reactome | IL7R [clathrin-coated vesicle membrane]Reactome | IL7R [clathrin-coated vesicle membrane]

Clathrin recruits auxilins to the clathrin-coated vesicle (Homo sapiens) * PI(4)P:p-T156 AP-2:clathrin:ITSNs:EPS15:REPS1:SGIP1: ...
more infohttps://reactome.org/content/detail/R-HSA-9028500

Reactome | N-WASP [cytosol]Reactome | N-WASP [cytosol]

Clathrin recruits auxilins to the clathrin-coated vesicle (Homo sapiens) * PI(4)P:p-T156 AP-2:clathrin:ITSNs:EPS15:REPS1:SGIP1: ...
more infohttps://reactome.org/content/detail/R-HSA-201873

An Auxilin-Like J-Domain Protein, JAC1, Regulates Phototropin-Mediated Chloroplast Movement in Arabidopsis | Plant PhysiologyAn Auxilin-Like J-Domain Protein, JAC1, Regulates Phototropin-Mediated Chloroplast Movement in Arabidopsis | Plant Physiology

5). Auxilins have nonconserved clathrin-binding regions at the N terminus and all except the worm auxilin contain an Asp-Pro- ... 5C), although cow, worm, and yeast auxilins do not have this motif. At present, it is not clear whether the FxDxF motif and ... Across the C-terminal domain, JAC1 shares between 25% and 31% amino acid similarity with animal and yeast auxilins (Fig. 5B). A ... However, their N-terminal domains have no homology to other nonplant auxilins and no other recognizable domain, but a short ...
more infohttp://www.plantphysiol.org/content/139/1/151?ijkey=5d4ea0f868e60d199cf68d65efadc4eea3ca3fe1&keytype2=tf_ipsecsha

Search Results | RUPSearch Results | RUP

In contrast, we could not detect auxilins in abortive pits or at any time during coated pit assembly. We previously showed that ... These bursts contain a very small number of auxilins, and even four to six molecules are sufficient to mediate uncoating. ...
more infohttps://rupress.org/jcb/search-results?f_SemanticFilterTopics=paracrine

Search Results | RUPSearch Results | RUP

In contrast, we could not detect auxilins in abortive pits or at any time during coated pit assembly. We previously showed that ... These bursts contain a very small number of auxilins, and even four to six molecules are sufficient to mediate uncoating. ...
more infohttps://rupress.org/jcb/search-results?f_SemanticFilterTopics=dictyostelium

Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating.  - PubMed - NCBIStructure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating. - PubMed - NCBI

Auxilins/metabolism*. *Auxilins/ultrastructure. *Cattle. *Clathrin/chemistry. *Clathrin/metabolism*. *Clathrin/ultrastructure* ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/15502813

Profiling the downstream genes of tumor suppressor PTEN in lung cancer cells by complementary DNA microarray<...Profiling the downstream genes of tumor suppressor PTEN in lung cancer cells by complementary DNA microarray<...

TY - JOUR. T1 - Profiling the downstream genes of tumor suppressor PTEN in lung cancer cells by complementary DNA microarray. AU - Hong, Tse-Ming. AU - Yang, P. C.. AU - Peck, K.. AU - Chen, J. J.W.. AU - Yang, S. C.. AU - Chen, Y. C.. AU - Wu, C. W.. PY - 2000/1/1. Y1 - 2000/1/1. N2 - The phosphatase and tensin homology deleted on chromosome 10 (PTEN) is a tumor suppressor gene with sequence homology to tyrosine phosphatases and the cytoskeletal proteins tensin and auxilin. PTEN has recently been shown to inhibit cell migration and the spreading and formation of focal adhesions. This study investigated the role of PTEN in carcinoma invasion in a lung-cancer cell line and examined the downstream genes regulated by PTEN. We have previously established a cell-line model in human lung adenocarcinoma with different invasive abilities and metastatic potentials. Examining PTEN gene expression in these cell lines, we found that a homozygous deletion in exon 5 is associated with high invasive ability. ...
more infohttps://researchoutput.ncku.edu.tw/en/publications/profiling-the-downstream-genes-of-tumor-suppressor-pten-in-lung-c

Experts and Doctors on  in United StatesExperts and Doctors on in United States

auxilins*insect hormones*clathrin heavy chains*embryonic structures*molting*frizzled receptors*sense organs*paleography*animal ...
more infohttp://www.labome.org/locale/experts-and-doctors-on-in-united-states-22015.html

SNARE Proteins | Profiles RNSSNARE Proteins | Profiles RNS

Auxilins. *Caveolins. *Class III Phosphatidylinositol 3-Kinases. *Clathrin. *Coat Protein Complex I ...
more infohttps://profiles.umassmed.edu/display/122367

dict.md | Adict.md | A

Auxilins [Chemical/Ingredient]. Auxilium Pharmaceuticals Inc. Brand of Testosterone. AUXILLARY POWER SUPPLY (AC OR DC) FOR ...
more infohttp://en.dict.md/A/520

Antigens, CD63 | Profiles RNSAntigens, CD63 | Profiles RNS

Auxilins. *Caveolins. *Class III Phosphatidylinositol 3-Kinases. *Clathrin. *Coat Protein Complex I ...
more infohttps://profiles.rush.edu/display/26011

Reactome | sh3kbp1 [cytosol]Reactome | sh3kbp1 [cytosol]

Clathrin recruits auxilins to the clathrin-coated vesicle (Danio rerio) * PI(4)P:p-T156 AP-2:clathrin:ITSNs:EPS15:REPS1:SGIP1: ...
more infohttp://www.reactome.org/content/detail/R-DRE-182957

Reactome | AP2B1 [cytosol]Reactome | AP2B1 [cytosol]

Clathrin recruits auxilins to the clathrin-coated vesicle (Bos taurus) * PI(4)P:p-T156 AP-2:clathrin:ITSNs:EPS15:REPS1:SGIP1: ...
more infohttps://reactome.org/content/detail/R-BTA-182166
  • In this study, we show that HeLa cells also express auxilin 1, previously thought to be neuron specific, and that both auxilins need to be depleted for inhibition of clathrin-mediated endocytosis and intracellular sorting. (biomedsearch.com)
  • Depleting both auxilins cause an approximately 50% reduction in the number of clathrin-coated pits at the plasma membrane but enhances the association of clathrin and adaptors with intracellular membranes. (biomedsearch.com)
  • Together, these findings indicate that the two auxilins have overlapping functions and that they not only facilitate the uncoating of CCVs but also prevent the formation of nonproductive clathrin cages in the cytosol. (biomedsearch.com)