A family of proteins that play a role as cofactors in the process of CLATHRIN recycling in cells.
Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.
Blue-light receptors that regulate a range of physiological responses in PLANTS. Examples include: PHOTOTROPISM, light-induced stomatal opening, and CHLOROPLAST movements in response to changes in light intensity.
Flavoproteins that function as circadian rhythm signaling proteins in ANIMALS and as blue-light photoreceptors in PLANTS. They are structurally-related to DNA PHOTOLYASES and it is believed that both classes of proteins may have originated from an earlier protein that played a role in protecting primitive organisms from the cyclical exposure to UV LIGHT.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
A species of nematode that is widely used in biological, biochemical, and genetic studies.
Neurodegenerative disorders involving deposition of abnormal tau protein isoforms (TAU PROTEINS) in neurons and glial cells in the brain. Pathological aggregations of tau proteins are associated with mutation of the tau gene on chromosome 17 in patients with ALZHEIMER DISEASE; DEMENTIA; PARKINSONIAN DISORDERS; progressive supranuclear palsy (SUPRANUCLEAR PALSY, PROGRESSIVE); and corticobasal degeneration.
Proteins from the nematode species CAENORHABDITIS ELEGANS. The proteins from this species are the subject of scientific interest in the area of multicellular organism MORPHOGENESIS.
Microtubule-associated proteins that are mainly expressed in neurons. Tau proteins constitute several isoforms and play an important role in the assembly of tubulin monomers into microtubules and in maintaining the cytoskeleton and axonal transport. Aggregation of specific sets of tau proteins in filamentous inclusions is the common feature of intraneuronal and glial fibrillar lesions (NEUROFIBRILLARY TANGLES; NEUROPIL THREADS) in numerous neurodegenerative disorders (ALZHEIMER DISEASE; TAUOPATHIES).
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Individuals licensed to practice medicine.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Persons whose profession is to give legal advice and assistance to clients and represent them in legal matters. (American Heritage Dictionary, 3d ed)
Promotion and protection of the rights of children; frequently through a legal process.
Predetermined sets of questions used to collect data - clinical data, social status, occupational group, etc. The term is often applied to a self-completed survey instrument.
Attitudes of personnel toward their patients, other professionals, toward the medical care system, etc.
Bites inflicted by humans.
A MARVEL domain-containing protein found in the presynaptic vesicles of NEURONS and NEUROENDOCRINE CELLS. It is commonly used as an immunocytochemical marker for neuroendocrine differentiation.
Works about controlled studies which are planned and carried out by several cooperating institutions to assess certain variables and outcomes in specific patient populations, for example, a multicenter study of congenital anomalies in children.
A malignant neoplasm that may be classified either as a glioma or as a primitive neuroectodermal tumor of childhood (see NEUROECTODERMAL TUMOR, PRIMITIVE). The tumor occurs most frequently in the first decade of life with the most typical location being the cerebellar vermis. Histologic features include a high degree of cellularity, frequent mitotic figures, and a tendency for the cells to organize into sheets or form rosettes. Medulloblastoma have a high propensity to spread throughout the craniospinal intradural axis. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2060-1)
An abnormal protein with unusual thermosolubility characteristics that is found in the urine of patients with MULTIPLE MYELOMA.
Primary or metastatic neoplasms of the CEREBELLUM. Tumors in this location frequently present with ATAXIA or signs of INTRACRANIAL HYPERTENSION due to obstruction of the fourth ventricle. Common primary cerebellar tumors include fibrillary ASTROCYTOMA and cerebellar HEMANGIOBLASTOMA. The cerebellum is a relatively common site for tumor metastases from the lung, breast, and other distant organs. (From Okazaki & Scheithauer, Atlas of Neuropathology, 1988, p86 and p141)
Glioma derived from EPENDYMOGLIAL CELLS that tend to present as malignant intracranial tumors in children and as benign intraspinal neoplasms in adults. It may arise from any level of the ventricular system or central canal of the spinal cord. Intracranial ependymomas most frequently originate in the FOURTH VENTRICLE and histologically are densely cellular tumors which may contain ependymal tubules and perivascular pseudorosettes. Spinal ependymomas are usually benign papillary or myxopapillary tumors. (From DeVita et al., Principles and Practice of Oncology, 5th ed, p2018; Escourolle et al., Manual of Basic Neuropathology, 2nd ed, pp28-9)
Works about pre-planned studies of the safety, efficacy, or optimum dosage schedule (if appropriate) of one or more diagnostic, therapeutic, or prophylactic drugs, devices, or techniques selected according to predetermined criteria of eligibility and observed for predefined evidence of favorable and unfavorable effects. This concept includes clinical trials conducted both in the U.S. and in other countries.

Identification of domain required for catalytic activity of auxilin in supporting clathrin uncoating by Hsc70. (1/38)

During clathrin-mediated endocytosis Hsc70, supported by the J-domain protein auxilin, uncoats clathrin-coated vesicles. Auxilin contains both a clathrin-binding domain and a J-domain that binds Hsc70, and it has been suggested that these two domains are both necessary and sufficient for auxilin activity. To test this hypothesis, we created a chimeric protein consisting of the J-domain of auxilin linked to the clathrin-binding domain of the assembly protein AP180. This chimera supported uncoating, but unlike auxilin it acted stoichiometrically rather than catalytically because, like Hsc70, it remained associated with the uncoated clathrin. This observation supports our proposal that Hsc70 chaperones uncoated clathrin by inducing formation of a stable Hsc70-clathrin-AP complex. It also shows that Hsc70 acts by dissociating individual clathrin triskelions rather than cooperatively destabilizing clathrin-coated vesicles. Because the chimera lacks the C-terminal subdomain of the auxilin clathrin-binding domain, it seemed possible that this subdomain is required for auxilin to act catalytically, and indeed its deletion caused auxilin to act stoichiometrically. In contrast, deletion of the N-terminal subdomain weakened auxilin-clathrin binding and prevented auxilin from polymerizing clathrin. Therefore the C-terminal subdomain of the clathrin-binding domain of auxilin is required for auxilin to act catalytically, whereas the N-terminal subdomain strengthens auxilin-clathrin binding.  (+info)

Molecular and functional characterization of clathrin- and AP-2-binding determinants within a disordered domain of auxilin. (2/38)

Uncoating of clathrin-coated vesicles requires the J-domain protein auxilin for targeting hsc70 to the clathrin coats and for stimulating the hsc70 ATPase activity. This results in the release of hsc70-complexed clathrin triskelia and concomitant dissociation of the coat. To understand the complex role of auxilin in uncoating and clathrin assembly in more detail, we analyzed the molecular organization of its clathrin-binding domain (amino acids 547-813). CD spectroscopy of auxilin fragments revealed that the clathrin-binding domain is almost completely disordered in solution. By systematic mapping using synthetic peptides and by site-directed mutagenesis, we identified short peptide sequences involved in clathrin heavy chain and AP-2 binding and evaluated their significance for the function of auxilin. Some of the binding determinants, including those containing sequences 674DPF and 636WDW, showed dual specificity for both clathrin and AP-2. In contrast, the two DLL motifs within the clathrin-binding domain were exclusively involved in clathrin binding. Surprisingly, they interacted not only with the N-terminal domain of the heavy chain, but also with the distal domain. Moreover, both DLL peptides proved to be essential for clathrin assembly and uncoating. In addition, we found that the motif 726NWQ is required for efficient clathrin assembly activity. Auxilin shares a number of protein-protein interaction motifs with other endocytic proteins, including AP180. We demonstrate that AP180 and auxilin compete for binding to the alpha-ear domain of AP-2. Like AP180, auxilin also directly interacts with the ear domain of beta-adaptin. On the basis of our data, we propose a refined model for the uncoating mechanism of clathrin-coated vesicles.  (+info)

Auxilin-dynamin interactions link the uncoating ATPase chaperone machinery with vesicle formation. (3/38)

The large GTPase dynamin is required for budding of clathrin-coated vesicles from the plasma membrane, after which the clathrin coat is removed by the chaperone Hsc70 and its cochaperone auxilin. Recent evidence suggests that the GTP-bound form of dynamin may recruit factors that execute the fission reaction. Here, we show that dynamin:GTP binds to Hsc70 and auxilin. We mapped two domains within auxilin that interact with dynamin, and these domains inhibit endocytosis when overexpressed in HeLa cells or when added in a permeable cell assay. The inhibition is not due to impairment of clathrin uncoating or to altered clathrin distribution in cells. Thus, in addition to its requirement for clathrin uncoating, our results show that auxilin also acts during the early steps of clathrin-coated vesicle formation. The data suggest that dynamin regulates the action of molecular chaperones in vesicle budding during endocytosis.  (+info)

Experimentally biased model structure of the Hsc70/auxilin complex: substrate transfer and interdomain structural change. (4/38)

A model structure of the Hsc70/auxilin complex has been constructed to gain insight into interprotein substrate transfer and ATP hydrolysis induced conformational changes in the multidomain Hsc70 structure. The Hsc70/auxilin system, which is a member of the Hsp70/Hsp40 chaperone system family, uncoats clathrin-coated vesicles in an ATP hydrolysis-driven process. Incorporating previous results from NMR and mutant binding studies, the auxilin J-domain was docked into the Hsc70 ATPase domain lower cleft using rigid backbone/flexible side chain molecular dynamics, and the Hsc70 substrate binding domain was docked by a similar procedure. For comparison, J-domain and substrate binding domain docking sites were obtained by the rigid-body docking programs DOT and ZDOCK, filtered and ranked by the program ClusPro, and relaxed using the same rigid backbone/flexible side chain dynamics. The substrate binding domain sites were assessed in terms of conserved surface complementarity and feasibility in the context of substrate transfer, both for auxilin and another Hsp40 protein, Hsc20. This assessment favors placement of the substrate binding domain near D152 on the ATPase domain surface adjacent to the J-domain invariant HPD segment, with the Hsc70 interdomain linker in the lower cleft. Examining Hsc70 interdomain energetics, we propose that long-range electrostatic interactions, perhaps due to a difference in the pKa values of bound ATP and ADP, could play a major role in the structural change induced by ATP hydrolysis. Interdomain electrostatic interactions also appear to play a role in stimulation of ATPase activity due to J-domain binding and substrate binding by Hsc70.  (+info)

Visualization of the binding of Hsc70 ATPase to clathrin baskets: implications for an uncoating mechanism. (5/38)

Clathrin assembly into coated pits and vesicles is promoted by accessory proteins such as auxilin and AP180, and disassembly is effected by the Hsc70 ATPase. These interactions may be mimicked in vitro by the assembly and disassembly of clathrin "baskets." The chimera C58J is a minimal construct capable of supporting both reactions; it consists of the C58 moiety of AP180, which facilitates clathrin assembly, fused with the J domain of auxilin, which recruits Hsc70 to baskets. We studied the process of disassembly by using cryo-electron microscopy to identify the initial binding site of Hsc70 on clathrin-C58J baskets at pH 6, under which conditions disassembly does not proceed further. Hsc70 interactions involve two sites: (i) its major interaction is with the sides of spars of the clathrin lattice, close to the triskelion hubs and (ii) there is another interaction at a site at the N-terminal hooks of the clathrin heavy chains, presumably via the J domain of C58J. We propose that individual triskelions may be extricated from the clathrin lattice by the concerted action of up to six Hsc70 molecules, which intercalate between clathrin leg segments, prying them apart. Three Hsc70s remain bound to the dissociated triskelion, close to its trimerization hub.  (+info)

An auxilin-like J-domain protein, JAC1, regulates phototropin-mediated chloroplast movement in Arabidopsis. (6/38)

The ambient-light conditions mediate chloroplast relocation in plant cells. Under the low-light conditions, chloroplasts accumulate in the light (accumulation response), while under the high-light conditions, they avoid the light (avoidance response). In Arabidopsis (Arabidopsis thaliana), the accumulation response is mediated by two blue-light receptors, termed phototropins (phot1 and phot2) that act redundantly, and the avoidance response is mediated by phot2 alone. A mutant, J-domain protein required for chloroplast accumulation response 1 (jac1), lacks the accumulation response under weak blue light but shows a normal avoidance response under strong blue light. In dark-adapted wild-type cells, chloroplasts accumulate on the bottom of cells. Both the jac1 and phot2 mutants are defective in this chloroplast movement in darkness. Positional cloning of JAC1 reveals that this gene encodes a J-domain protein, resembling clathrin-uncoating factor auxilin at its C terminus. The amounts of JAC1 transcripts and JAC1 proteins are not regulated by light and by phototropins. A green fluorescent protein-JAC1 fusion protein showed a similar localization pattern to green fluorescent protein alone in a transient expression assay using Arabidopsis mesophyll cells and onion (Allium cepa) epidermal cells, suggesting that the JAC1 protein may be a soluble cytosolic protein. Together, these results suggest that JAC1 is an essential component of phototropin-mediated chloroplast movement.  (+info)

Depletion of GAK/auxilin 2 inhibits receptor-mediated endocytosis and recruitment of both clathrin and clathrin adaptors. (7/38)

Cyclin G-associated kinase (GAK/auxilin 2), the ubiquitous form of the neuronal-specific protein auxilin 1, is an essential cofactor for the Hsc70-dependent uncoating of clathrin-coated vesicles. We have now investigated the effect of knocking down GAK in HeLa cells by vector-based small hairpin RNA. Functionally, depletion of GAK caused a marked decrease in internalization of both transferrin and epidermal growth factor and altered mannose 6-phosphate receptor trafficking, but had little effect on the recycling of transferrin receptor back to the plasma membrane. Structurally, depletion of GAK caused a marked reduction in perinuclear clathrin associated with the trans-Golgi network and in the number of clathrin-coated pits on the plasma membrane, and reduced clathrin exchange on the few clathrin-coated pits that remained. Surprisingly, while clathrin depletion does not prevent adaptors from assembling on the membrane, depletion of GAK caused a dramatic reduction in AP2 and epsin on the plasma membrane and AP1 and GGA at the trans-Golgi network. A similar effect was caused by expression of a dominant negative Hsp70 mutant. These results suggest that GAK, in conjunction with Hsc70, not only uncoats clathrin-coated vesicles and induces clathrin exchange on clathrin-coated pits, but also mediates binding of clathrin and adaptors to the plasma membrane and the trans-Golgi network.  (+info)

Structural basis of interdomain communication in the Hsc70 chaperone. (8/38)

Hsp70 family proteins are highly conserved chaperones involved in protein folding, degradation, targeting and translocation, and protein complex remodeling. They are comprised of an N-terminal nucleotide binding domain (NBD) and a C-terminal protein substrate binding domain (SBD). ATP binding to the NBD alters SBD conformation and substrate binding kinetics, but an understanding of the mechanism of interdomain communication has been hampered by the lack of a crystal structure of an intact chaperone. We report here the 2.6 angstroms structure of a functionally intact bovine Hsc70 (bHsc70) and a mutational analysis of the observed interdomain interface and the immediately adjacent interdomain linker. This analysis identifies interdomain interactions critical for chaperone function and supports an allosteric mechanism in which the interdomain linker invades and disrupts the interdomain interface when ATP binds.  (+info)

Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Untuk mengamankan file website dan database pada xampp gak harus menginstallnya di local disk D, menginstallnya di local disk C pun bisa
Sorry gan klo :repost moga-moga bisa bikin :ngakak PREMAN A: Eh, loe tau gak kenapa preman-preman sini pada nunduk tiap liat gue? B: Gak... Gak tau bang. (sambil ketakutan) A: Karena gue pendek tau!! :ngakaks DITILANG POLISI A: Mana surat-suratmu?? B: Maaf pak, saya sekarang udah gak pake surat, saya sekarang pake e-mail A: :capedes MIMPI A: Gue mimpi dikejar-kejar anjing B: Udahlah, cuma mimpi. A
Nahhh… kalo spesifikasi yang Sony Ericsson Xperia X1 ama T-Mobile G1 gak usah gw tulis ya? he..he.. silahkan bandingin photo-photonya dulu deh… nahh.. kalo spek sih gw yakin karena harga selangit juga gak ngecewakan.. tapi kalo urusan handal nggak nya.. gw gak bisa nulis disini.. daripada ntr gw di kira sok teuuu… mending nanya langsung ama yang udah pegang ya bro? soal Harga Nokia N97 juga, meding cek langsung ke Nokia centernya aja langsung ya? wokke.. nahhh… buat email2 dari bro Anjaz, sis Icha, Bimo, Kuda nil, Raka, Toni Abdilah.. dan beberapa lagi yang namanya susah binti ribet… sorry gw cuma bisa review segini.. soalnya mo kerja dulu gw.. he..he ...
Hmm..sama laa ngan adik sy..dia ngan famili mentua dia dh booked tiket ke PADANG ujung tahun lepas lagik..Nak pi ujung November ni. Nak tak nak..terpaksa ler pi gak. Kalo tukar ke tempat lain..kena tmbah byk duit lagik ...
We describe a 120-kDa protein (pp120) that is phosphorylated on tyrosine in cells attached to fibronectin-coated surfaces. The protein appears to be located in focal contacts where it codistributes with beta 1 integrins. pp120 is distinct from the beta 1 subunit of integrins and from vinculin and al …
Chloroplasts change their position in a cell in response to environmental light conditions (Wada et al., 1993, 2003). Low-fluence rate light induces movement of chloroplasts toward the irradiated area, resulting in chloroplast accumulation at the front face of the cell (accumulation response). Conversely, under high-fluence rate light, chloroplasts move to the anticlinal wall of the cell to avoid photodamage (avoidance response; Kasahara et al., 2002). Chloroplast photorelocation movement is found in several photosynthetic plant species, including yellow and green algae, mosses, ferns, and flowering plants. In most plant species, chloroplast movement is induced by irradiation with blue light, although it is also induced by red light in some cryptogam plants (Wada et al., 1993, 2003). The flowering plant Arabidopsis (Arabidopsis thaliana) has two types of blue-light photoreceptor, cryptochromes (cry1 and cry2) and phototropins (phot1 and phot2). Cryptochrome is a flavoprotein similar to the ...
A total of 89 J-domain proteins were identified in the genome of the model flowering plant Arabidopsis thaliana. The deduced amino acid sequences of the J-domain proteins were analyzed for an assortment of structural features and motifs. Based on the results of sequence comparisons and structure and …
SMAP2 immunoprecipitated clathrin and AP-1 through a putative clathrin-binding domain and a CALM-binding domain, and SMAP2 mutants that did not interact with clathrin or AP-1 could not localize to recycling ...
Begini cara proses kredit motor yang paling gampang gak bikin kamu ribet dan kesusahan. ✓Ajukan Kredit ✓Isi Form✓Sur vey & Verifikasi ✓Motor & STNK Dikirim
Eh iya Mel, elu jadi di BalSud kan ya kawinannya? FYI, kemarin ini gue ke kondangan di situ, lagi2 sound systemnya kok gak enak ya. Mungkin elu kudu nambah 20rb watt lagi deh. Soalnya suara MC itu gak kedengeran, padahal MC-nya itu udah sampe teriak2 loh. Dan MC ini udah biasa di hotel dan bagus. Sayang deh kalo lu udah bayar MC mahal2, tau2 nanti gak kedengeran. ReplyDelete ...
Patot ar Ain debabbbb..sbb bapak dia pon debab gak..hehehe..bapak ain ni sbenarnye abang yan....ermmmAbg yg ketiga...tp dia anak yg ke-9 drpd 10 org adik bradik...Dia ni kawen tahun 2007...Alhamdulillah dah ade 2 org anak....yg si Ain ni anak yg ke-2...anak yg pertama lelaki....namenye... ...
Nucleotide binding to the 70 kDa heat-shock cognate protein (Hsc70) from mung bean seeds and pig brain was investigated, as well as the clathrin uncoating activity of Hsc70 in the presence of these nucleotides. The two enzymes were found to behave identically. ATP bound to two different forms of Hsc70, with dissociation constants of 1.1±0.1 µM and 1.4±0.7 mM respectively at 25 °C. This corresponds to ΔG0´ = -34 and -16 kJ/mol respectively. From the temperature-dependence of the dissociation constant of the high-affinity site, ΔH0´ was calculated to -36±2 kJ/mol. This gives ΔS0´ = 6.7 J/mol per K. Adenosine 5´-[γ-thio]triphosphate, ADP, adenosine 5´-[β,γ-imino]triphosphate and adenosine 5´-[β,γ-methylene]triphosphate showed dissociation constants of 2.3, 11, 31 and 284 µM respectively. The order of affinities corresponded to the order of effectiveness in uncoating of pig brain coated vesicles. The implications of these findings for the mechanism of Hsc70 action are discussed. ...
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Udah lama gak update blog, mestinya nulis tentang update sekolah Anelka yaa hehe.. *kumpulin niat* Anw gak terasa banget duedatenya Mimi semakin dekat.. Salah satu yang mau dibenahi menyambut kelahiran itu mobil. Karena mobil yang ada cuma 2 row, dan kalo ditambah 1 carseat lagi jadi sumpek pek gak muat apa2 lagi.. Mana lagi, sampai…
alhamdulillah....harap sumenye okay sentiasa..eida jgn lupe doakan utk nis key..nis pn sentiasa doakan baby eida pn okay gak..yeke baby girl??he..he..tapi nape ramai yg ramal nis bakal dpt boy lagik??kalender cina pn camtu gak..kire suprise nih..!! ...
Remaja putri pelaku sex pranikah pasti bingung bin kelabakan kalo udah telat menstruasi, masalahnya selama ini telat menstruasi selalu identik dengan kehamilan, padahal belum tentu lho, apalagi kalo gak berhubungan sex sama sekali, masak gak ngapa-ngapain tiba-tiba hamil? (sumber: juraganmedis.com). Telat menstruasi atau bahasa kerennya amenorrhea sering terjadi pada banyak wanita, terutama pada remaja putri…
um..smlm da bli tiket raya..hoho..horey..setel satu hal..blom puasa lagik da sebuk pk pasal raya..hoho..tp ok gak..sementara tiket masih murah nih..tu pon da
Joosen, Margot C. W.; Brouwers, Evelien P. M.; van Beurden, Karlijn M.; Terluin, Berend; Ruotsalainen, Jani H.; Woo, Jong-Min; Choi, Kyeong-Sook; Eguchi, Hisashi; Moriguchi, Jiro; van der Klink, Jac J. L.; van Weeghel, Jaap ...
all of a sudden the older one went in his litter box and peed a little, then jumped on a sofa in our sun room and peed a little. (Thank goodness we have a blanket/sheet on all sofas so we dont have too much fur on them. He then went on the sofa in the Living Room and did the same ...
TY - JOUR. T1 - Influence of the cytosolic carboxy termini of human bl and b2 kinin receptors on ligand internalization. AU - Faussner, A.. AU - Proud, D.. AU - Towns, M.. AU - Bathon, J.. PY - 1996. Y1 - 1996. N2 - To examine the role of the cytoplasmic carboxy termini of human B1 and B2 kinin receptors (B1R and B2R) in the internalization of their respective ligands, des-ArglO-kallidin (DAK) and bradykinin (BK), both wild-type receptors, as well as a truncated B2R and chimeric receptors, were expressed as stable clones in CHO cells. Incubation of [3H]-BK at 37°C with cells expressing wild type B2R (Kd = 2nM) resulted in a pronounced and rapid ligand internalization (-80% after 10 min). By contrast incubation of [3H]DAK with cells expressing B1R (Kd = 0.4 nM) resulted in a modest, slow internalization of the ligand (. AB - To examine the role of the cytoplasmic carboxy termini of human B1 and B2 kinin receptors (B1R and B2R) in the internalization of their respective ligands, ...
TY - JOUR. T1 - AtJ6, a unique J-domain protein from Arabidopsis thaliana. AU - Kroczyńska, Barbara. AU - Coop, Nichole E.. AU - Miernyk, Jan A.. PY - 2000/2/7. Y1 - 2000/2/7. N2 - An Arabidopsis thaliana cDNA encoding a protein, AtJ6, related to Escherichia coli DnaJ was sequenced. Translation of the At J6 nucleotide sequence yields a protein with an N-terminal J-domain, but which lacks the G/F, and C-rich domains characteristic of DnaJ. In addition to the J-domain, one region of the sequence has homology with the prokaryotic FtsA protein. The remainder of the At J6 sequence is not notably related to anything in the sequence databases. The At J6 sequence represents a unique member of the A. thaliana J-domain protein family. The results of Northern analysis revealed that the At J6 mRNA is expressed in all organs of A. thaliana; expression was high in leaves, flowers, and siliques, and low in roots. The results of Southern analysis are consistent with a single unique gene.. AB - An Arabidopsis ...
Researchers will formulate a model that predicts the spatiotemporal variability in groundwater vulnerability to UO&G-associated chemicals. This statistical model will be trained with calculations of a physically based, hydrologic model that simulates the probability of overlap between capture zones of drinking-water wells and UO&G contaminant sources in Susquehanna County, PA and Belmont County, OH. Once calibrated, the resultant vulnerability index model will be tested against water-quality data collected in a large-scale sampling campaign of 500 homes across four additional PA and OH counties. Water samples will be analyzed for a broad range of inorganic and organic compounds, including UO&G-related contaminants that cannot be detected by conventional analytical techniques. Our studies will culminate with a case-control study of 8,000 neonates in PA and OH to determine whether exposure to UO&G contaminants (as estimated by our vulnerability index model) is associated with changes in the ...
Specific receptor-mediated endocytosis pathways can be investigated with fluorescent ligands including pHrodo™ dyes targeted to common receptors, including, EGF, LDL, and transferrin.. The most convenient, no-wash assay format uses pHrodo™ Red or pHrodo™ Green to allow discrimination of stages in the endocytic pathway from early endosome to lysosome formation with no quench or wash required.. pHrodo™ dyes are essentially non-fluorescent at neutral pH and exhibit increasing signal with a red or green readout respectively as the pH decreases. The increase in fluorescent signal can be used to monitor progression in the endocytic pathway.. ...
Pas kemaren FA, temen gwe share tentang ini.. gwe bahkan baru menyadari ada ayat ini di Alkitab, bahkan opung Daud juga sadar dari zaman dahulu kalau memang kadang kita bisa melakukan sesuatu tanpa kita sadari, dan opung Daud sadar kalau yang dia gak sadari, gak bisa dia kontrol, dan He asked God, untuk membebaskan dia dari segala hal yang dia gak sadarin, yang mungkin di hari-hari ke depan akan dia lakukan, akan dia ucapkan ...
gimana sih caranya bikin web yang keren gini, ajarin dunk mas..:D. kunjungi web saya juga mas…kasih coment, biar bisa lebih maju dalam nge bLOG..huehue…paling engga sesama rekan kerja bisa bagi bagi ilmu, meskipun berbeda pulau mas..hehehe... oh ya..belum kenal udah sok akrab, tapi gak apa kan mas..dari pada sok musuhan..hehehe..sekali lagi..salam kenal mas dari RuL di ujung pulau sumatera di Bumi Serambi Mekkah. keep update mas…:). ...
30 Ogos plak bday girl... Sape lg yg gegirl dlm umah ni kan... Ofkos la mama...hahaha... Tp yg geram + nk merajuk tp xjd, maghrib ms buka puasa tu bru la Abg igt nk wish..huhuhu sabo je la kan... Seb baik ada alasan yg kukuh...xpe la, dimaafkan... Utk bday girl plak, msk sendiri gak... Wat roti jala ngan kuzi ayam... Roti jala tu pki tepung blue key...hahaha punya la xcek brg dapur, rupanye tepung gandum abis dah.. Hentam je la... Sdp gak tau ...
:selamat SELAMAT DATANG :selamat BUAT AGAN/AGANWATI YANG BAIK GAK BAKALAN LUPA SAMA KOMENGNYA SERTA :rate5 DAN :cendolbig Semoga Gak :repost Indonesia negara tercinta kita ini berada dalam cicin api pasifik, atau kata mudahnya memiliki banyak gunung berapi, gunung berapi yang dianugrahkan di negara kita memang memiliki 2 sisi, yaitu anugrah karena bisa menyuburkan tanah:malus, serta bencana jika k
Sering mengeluh soal barang bawaan dalam tas sangat berat? Toss dulu sama saya! Gak tau kenapa selalu bertahan untuk bawa tas yang berat ini. Apa karena pa
Jangan cari masalah sama Eminem lah, kalau gak mau dijadiin bahan Lirik Eminem di album barunya Kamikaze. 13 lagu muantap jiwa pembantai alay
Recent mooring observations of ocean temperature provide the first high resolution, long term record of temperature variability in the Makassar Strait of the Indonesian Seas. The mooring observations span the entire cycle of the strong 1997/1998 El Nino. A high correlation (r = 0.67) is found between variability in the average thermocline temperature, to variability in the southward Makassar volume transport: during high (low) volume transport, the average temperature of the thermocline is also high(low). In addition, from nearly 15 years of XBT data, the Makassar thermocline temperature is shown to be highly correlated (r = 0.77) to SOI. This reveals that the Makassar temperature field - when coupled with the throughflow transmits the equatorial Pacific Fl Nino and La Nina temperature fluctuations into the Indian Ocean. The ENSO variability in the internal energy transport is calculated: 0.63 PW during the La Nina months of December 1996 through February 1997, and 0.39 PW during the El Nino ...
PhD Project - High resolution cryo-electron microscopy of clathrin cage networks at University of Warwick, listed on FindAPhD.com
Discuss the regulation of gene expression in HIV and the life cycle, and comment on the importance of these in the success - Essay Example Memory helper cells are differentially infected by the virus. The virus binds to the target cell using interactions between viral surface proteins (gp120) and cell surface proteins. The CD4 antigen, and the CXCR4 and CCR5 co-receptors on the host cell membrane are crucial in mediating viral entry into the cell. The interaction allows the viral and cellular membranes to fuse, so that the viral contents, including RNA and viral enzymes, enter the host cell. The viral capsid then uncoats and disassembles to release the 2 viral RNA strands, which are used to make complementary DNA by the viral enzyme reverse transcriptase. The virus cDNA is transported to the nucleus, where the viral integrase enzyme incorporates viral DNA into the host DNA, forming the provirus. The viral DNA genome remains latent in the cell for many years, as long as the T cell is quescent. ...
Characterization of clathrin-coated vesicles isolated from MPR-deficient fibroblasts. The material contained in fractions 8 to 10 of the density gradients sho
dan dia juga yg tolong aku balut..serius..dia yg tolong pegangkan kertas pembalut ni bila aku sibuk gunting seletep....hehehehe....senang..dah tak payah suh laki aku tolong aku ...
1st ni hakak nak ucapkan ribuan terima kasih pada Astro & Redmummy yang sudi panggil hakak sebagai jemputan tuk Project Alpha tempoh hari(korang dah undi tuk vote kak red)...Bukan hakak sorang jer yer dek non..kak rose, sid pun ada gak..Tapi kak ayu ngan yanok x dak...x per janji tempias2 tu tetap ada kan...GBM tetap kitorang bawak jugak..kan..Apsal hakak letak letak Rimmel punya make-up?Dan-dan jer tau...Coz, hakak baru dapat from sid..Walaupun kak red dah lama bagi kat hakak..tapi hakak x sempat nk jumpa dorang...Last2 sid gak tolong hantarkan kat offc hakak ...
Eunhyuk itu pelit. kalau pergi bareng dan masalah bayar - membayar dia suka bilang sama Leeteuk Hyung, bukannya kamu yang bayar?. Kata Leeteuk, Eunhyuk suka ngutang tapi lupa bayar. Ini juga yang dikatakan sama Sungmin (kalo gw gak salah ingat! Ingatan gw rada2 cuy… hehehehe) pas di Intimate Note! Dia ampe teriak, aku ini dongsaeng mu tapi kau gak pernah bayarin aku makan! Hahahaha… ...
Baru2 ni kat FB cam keluar game baru..baru tu tak baru gak la kot...aku pon cam dah seminggu gak dah kot main ni..hehe..nak kata addicted tu ntah laa..haaha.
Buy Korg NanoPad 2 (White) Slim-Line USB Pad Controller online from GAK.co.uk. Unbeatable prices and next day delivery from the UKs no1 instrument store. Order Today.
Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinsons disease, on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parsons lab at Kings College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinsons. Read more on her story here. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 23rd Feburary 2018. Apply now!. ...
Expression of GAK (DNAJC26) in adrenal gland tissue. Antibody staining with HPA027405, HPA027459 and HPA027463 in immunohistochemistry.
camtu la gak asyraaf time kat spital aritu... kesian tol.. rase cam nk menitik air mata time dia kena amik darah.. 3 kali cucuk br kuar darah... makan pn x lalu.. aku wat bubur pn dia x mkn.. last2 aku blend... br dia mkn.. mungkin time tu xde selera nk mkn kan. risau btl bile selera anak hilang.. macam2 vitamin la aku bg... gelabah gak kan kalu brdua ngan asben je time2 gni.. time ni gak la rase cam nk je mak ayah kita ade skali.. :D ...
p/s: tinggal 3 hari lagi nak final sem 2. kadang2 auni rasa blur bile tengok soklan past year. especially part anatomy. Auni tak tau orang lain macammane. tengok macam dorang sume dah ready. but semalam ade yang cerita kat auni menangis2 sebab stress sangat dengan exam ni. buat yang berkenaan, tak usah pikir2 lagi. sbenarnya jawapan tu sume da ade lam kepala kita. cuma kite terlupa mane kite letak kunci for jawapan tu. macammane nak dapatkan kunci tu balik? senang. RELAX..tarik nafas. when u feel stress, switch to thing which make u NOT stress. macam tgok citer industan ke (like i did yesterday), nyanyi ke (like i did yesterday), menari ke (like i did yesterday), berangan ke (like i did yesterday) or masak2 (like i did yesterday..err.. asal rase cam banyak semacam je auni buat semalam? em. aah.betol.. mmg banyakla). after that ull find the way.trust me. U WILL. k? otak pun nak relax gak. jangan pakse. otak tu makhluk tuhan gak. mesti die ade perasaan.kan? kan? (terbayang otak itu macam kartun ...
Sampai sana ahad kul 1 ptg.. Sampai2 sana je terus makan dulu.. Memang sedang elok jer waktu lunch kan.. Hujan... Sempat gak kami bakar ayam dan daging kambing.. Pastu kami cari tempat tido.. Sebelum tu ade call 2 chalet.. dua2 penuh.. Mase sampai sane tu mmg tawakal je la cari chalet.. Hampir 2 jam cari dan tanye hampir 20 chalet, akhirnya dapat gak... huhu.. La Bella Resort... Ngam2 je yg kosong adalah sebuah dorm utk 14 orang.. Alhamdulillah memang rezeki kami la ...
dan balik umah td,mak abah kecoh kangah dpt offer letter drpd poli..kangah yg spatutnye masuk ntok intake november telah dinaiktaraf ntok intake jun..klaw die trime offer tuh, kenalaa mendaftar next week! tekejut kot.. tp kalaw aq jadi die sah2 dah grab dah, sbb memula mmg x nmpk tp bile org sekeliling da grad,da keje..haii, x sabar nk grad cpat2 dan pgg duit sndrik..kire mmg rezeki die dpt offer gitu, jarang kot terjadi dan satu ag menurut pendapat org ramai, method poli ni sgt beza ngn universiti, sbb poli utamakan kemahiran dan perlu bersaing lebih dgn graduan universiti,jadi watpe nk buang masa satu sem kan.. tp tepulanglah kat tuan punya badan kan..susah gak nk get used sbb x penah dok asrama..dan skali dok asrama terus kena campak jauh..dilema gak tuh.. sdgkn aq pun x pernah dok luar selangor,tu yg teruja tuh! tp nilah dipanggil perubahan fasa kehidupan..dr kecik ke besar,dari murid skolah ke mahasiswa dan dari rumah ke hostel ...
Lanjut cerita soal shopping-shopping…Kemarin waktu pulang ke Semarang, aq sempet shopping-shopping…hehehehe…Cuma karena buru2, akhirnya barang2nya gak kefoto deh…zzzzz….Ternyata ibuku udah beliin kebutuhan-kebutuhan baju baby yang dasar…Jadi aq udah gak beli-beli lagi deh…Baju-baju lengan panjang, lengan pendek, tanpa lengan, popok kain, celana pendek, celana panjang, crelana pop, popok kain, gurita, alas ompol, dll udah dibeliin semua sama ibuku…Tadinya pengen beli yang merk Fluffy, Libby ato Nova…menurut referensi merk-merk itu yang banyak direkomendasiin…Ternyata udah dibeliin ibuku…ibuku beli yang lusinan, lebih murah jadinya…kainnya juga lumayan kok…Makasiiiihhh uti-nya dedek… =) Kemarin di Semarang akhirnya beli keperluan baby selain baju-baju basic…Aq belanja di Sejati baby shop Jl. Sompok…Rameeee deh disana…banyaak banget yang beli…tempatnya cukup nyaman…beda sama Kitty, klo di Kitty kan qt bisa ambil-ambil n pilih-pilih sendiri..Kalo di Sejati, qt ...
feeling much better. semalam akhirnya gue memutuskan untuk makan obat lodia dan minum obat maag plantacid buat ngilangin nyeri. lumayan hari ini enakan perut. nyeri berkurang dan ke belakang gak sesering tiga hari lalu yang mana kayak dikuras habis semua isi perut. perjuangan banget dah ...
Gw dibeliin mie ini sama nyokap sebelum booming, tanpa tahu kalo nih mie pedes banget, kirain mie goreng Korea pake gochujang biasa, kan kayanya enak tuh ya... Pas makan bujug dah *gw ga tahan pedes*. Kedua kali masak, bumbunya gak dipake semua, plus dicampur indomie goreng, lalu dibagi dua (makan berdua Benny), Tetep aja looooh gw ga tahaaan. Padahal rasanya enak banget menurut gw, mie nya kenyal2, tapi kenapa musti ekstrem gitu sih pedesnya????? Btw Benny menurut standar gw doyan cabe tapi dia kepedesan juga. Ckck. ReplyDelete ...
Tiba-tiba kepikiran soal ini : kamu gak perlu nikah untuk tahu dia pasanganmu yang sah atau bukan. Kamu cuma perlu seks. Haha. Maybe not. Kamu cukup tahu bahwa kamu seneng dia pasanganmu, kamu seneng mantanmu cuma sekedar mantan, syukur-syukur masih friendly as close as a sodara to you.
Stress xyah citer la...ditambah plak ngn batuk and selsema yg berterusan nie... Kerja opis yg xpenah surut... Bertambah2 stress.. But to be positive...saya juz brsyukur dgn apa yg dh ada sbb itu je yg saya dan family mampu buat... Majlis saya mungkin x segrand mana...tp insyaallah akan menjadi memori paling indah wat saya, bakal suami dan keluarga kami... Saya gak harap kwn2 dan sumer tetamu yg dtg akan kenyang, senang ati dan sama2 gembira meraikan kami... Insyaallah ...
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Clathrin recruits auxilins to the clathrin-coated vesicle (Bos taurus) Clathrin recruits auxilins to the clathrin-coated ... Clathrin recruits auxilins to the clathrin-coated vesicle (Canis familiaris) Clathrin recruits auxilins to the clathrin-coated ... Clathrin recruits auxilins to the clathrin-coated vesicle (Gallus gallus) Clathrin recruits auxilins to the clathrin-coated ... Clathrin recruits auxilins to the clathrin-coated vesicle (Sus scrofa) Clathrin recruits auxilins to the clathrin-coated ...
Both auxilins bind clathrin, adaptors (5?8) and dynamin (9). Their C-terminal J domain confers the specificity for binding to ... Relative abundance of auxilins To estimate the relative abundance of auxilin 1 and auxilin 2 in HeLa CCVs, we used our ... Depletion of both auxilins in HeLa cells resulted in a change in cell morphology with most cells appearing more rounded, which ... Auxilins / genetics, physiology*. Cell Membrane / metabolism*. Clathrin / chemistry*, metabolism*. Clathrin-Coated Vesicles / ...
Clathrin recruits auxilins to the clathrin-coated vesicle (Homo sapiens) * PI(4)P:p-T156 AP-2:clathrin:ITSNs:EPS15:REPS1:SGIP1: ...
Clathrin recruits auxilins to the clathrin-coated vesicle (Homo sapiens) * PI(4)P:p-T156 AP-2:clathrin:ITSNs:EPS15:REPS1:SGIP1: ...
5). Auxilins have nonconserved clathrin-binding regions at the N terminus and all except the worm auxilin contain an Asp-Pro- ... 5C), although cow, worm, and yeast auxilins do not have this motif. At present, it is not clear whether the FxDxF motif and ... Across the C-terminal domain, JAC1 shares between 25% and 31% amino acid similarity with animal and yeast auxilins (Fig. 5B). A ... However, their N-terminal domains have no homology to other nonplant auxilins and no other recognizable domain, but a short ...
In contrast, we could not detect auxilins in abortive pits or at any time during coated pit assembly. We previously showed that ... These bursts contain a very small number of auxilins, and even four to six molecules are sufficient to mediate uncoating. ...
Fingerprint Dive into the research topics where Joanna L Howarth is active. These topic labels come from the works of this person. Together they form a unique fingerprint. ...
Bartelink, I. H., Prideaux, B., Krings, G., Wilmes, L., Lee, P. R. E., Bo, P., Hann, B., Coppé, J. P., Heditsian, D., Swigart-Brown, L., Jones, E. F., Magnitsky, S., Keizer, R. J., de Vries, N., Rosing, H., Pawlowska, N., Thomas, S., Dhawan, M., Aggarwal, R., Munster, P. N. & 8 others, Esserman, L. J., Ruan, W., Wu, A. H. B., Yee, D., Dartois, V., Savic, R. M., Wolf, D. M. & van t Veer, L., Sep 11 2017, In : Breast Cancer Research. 19, 1, 107.. Research output: Contribution to journal › Article ...
Opladen, T., López-Laso, E., Cortès-Saladelafont, E., Pearson, T. S., Sivri, H. S., Yildiz, Y., Assmann, B., Kurian, M. A., Leuzzi, V., Heales, S., Pope, S., Porta, F., García-Cazorla, A., Honzík, T., Pons, R., Regal, L., Goez, H., Artuch, R., Hoffmann, G. F., Horvath, G. & 10 others, Thöny, B., Scholl-Bürgi, S., Burlina, A., Verbeek, M. M., Mastrangelo, M., Friedman, J., Wassenberg, T., Jeltsch, K., Kulhánek, J. & Kuseyri Hübschmann, O., May 26 2020, In : Orphanet Journal of Rare Diseases. 15, 1, 126.. Research output: Contribution to journal › Review article ...
Auxilins/metabolism*. *Auxilins/ultrastructure. *Cattle. *Clathrin/chemistry. *Clathrin/metabolism*. *Clathrin/ultrastructure* ...
auxilins*protoplasts*aquaglyceroporins*vitreoscilla*carbohydrate epimerases*aminoacyltransferases*atriplex*cotton fiber* ...
Auxilins. *Caveolins. *Class III Phosphatidylinositol 3-Kinases. *Clathrin. *Coat Protein Complex I ...
auxilins*insect hormones*clathrin heavy chains*embryonic structures*molting*frizzled receptors*sense organs*paleography*animal ...
Auxilins. *Caveolins. *Class III Phosphatidylinositol 3-Kinases. *Clathrin. *Coat Protein Complex I ...
Auxilins (in mammalian cells, auxilin1 (Aux1) and auxilin2 or GAK) need both a clathrin-binding area and a phosphatase and ... the fact that auxilin PTEN-like area interacts with a particular phosphoinositide in the coat-engulfed membrane which auxilins ...
Auxilins [Chemical/Ingredient]. Auxilium Pharmaceuticals Inc. Brand of Testosterone. AUXILLARY POWER SUPPLY (AC OR DC) FOR ...
Auxilins. *Caveolins. *Class III Phosphatidylinositol 3-Kinases. *Clathrin. *Coat Protein Complex I ...
Auxilins. *Caveolins. *Class III Phosphatidylinositol 3-Kinases. *Clathrin. *Coat Protein Complex I ...
Auxilins. *Caveolins. *Class III Phosphatidylinositol 3-Kinases. *Clathrin. *Coat Protein Complex I ...
The auxilins of yeast and C. elegans, which lack kinase and PTEN domains, uncoat clathrin-coated vesicles (CCVs) in vitro and ...
  • In this study, we show that HeLa cells also express auxilin 1, previously thought to be neuron specific, and that both auxilins need to be depleted for inhibition of clathrin-mediated endocytosis and intracellular sorting. (biomedsearch.com)
  • Depleting both auxilins cause an approximately 50% reduction in the number of clathrin-coated pits at the plasma membrane but enhances the association of clathrin and adaptors with intracellular membranes. (biomedsearch.com)
  • Together, these findings indicate that the two auxilins have overlapping functions and that they not only facilitate the uncoating of CCVs but also prevent the formation of nonproductive clathrin cages in the cytosol. (biomedsearch.com)
  • Auxilins (in mammalian cells, auxilin1 (Aux1) and auxilin2 or GAK) need both a clathrin-binding area and a phosphatase and tensin homologue (PTEN)-like area for effective recruitment to recently budded clathrin-coated vesicles4,7,8 (Prolonged Data Fig. 1a, Supplementary Video 1). (chinamicrospine.com)