The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)
Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).
An analgesic and antipyretic that has been given by mouth and as ear drops. Antipyrine is often used in testing the effects of other drugs or diseases on drug-metabolizing enzymes in the liver. (From Martindale, The Extra Pharmacopoeia, 30th ed, p29)
Compounds that are used in medicine as sources of radiation for radiotherapy and for diagnostic purposes. They have numerous uses in research and industry. (Martindale, The Extra Pharmacopoeia, 30th ed, p1161)
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Cell surface receptors that bind signalling molecules released by neurons and convert these signals into intracellular changes influencing the behavior of cells. Neurotransmitter is used here in its most general sense, including not only messengers that act to regulate ion channels, but also those which act on second messenger systems and those which may act at a distance from their release sites. Included are receptors for neuromodulators, neuroregulators, neuromediators, and neurohumors, whether or not located at synapses.
The sum of the weight of all the atoms in a molecule.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
Radioactive substances added in minute amounts to the reacting elements or compounds in a chemical process and traced through the process by appropriate detection methods, e.g., Geiger counter. Compounds containing tracers are often said to be tagged or labeled. (Hawley's Condensed Chemical Dictionary, 12th ed)
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Detection and counting of scintillations produced in a fluorescent material by ionizing radiation.
Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes.
2-Deoxy-D-arabino-hexose. An antimetabolite of glucose with antiviral activity.
Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.
Unstable isotopes of fluorine that decay or disintegrate emitting radiation. F atoms with atomic weights 17, 18, and 20-22 are radioactive fluorine isotopes.
The rate dynamics in chemical or physical systems.
Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.
An imaging technique using compounds labelled with short-lived positron-emitting radionuclides (such as carbon-11, nitrogen-13, oxygen-15 and fluorine-18) to measure cell metabolism. It has been useful in study of soft tissues such as CANCER; CARDIOVASCULAR SYSTEM; and brain. SINGLE-PHOTON EMISSION-COMPUTED TOMOGRAPHY is closely related to positron emission tomography, but uses isotopes with longer half-lives and resolution is lower.
A benzodiazepine with pharmacologic actions similar to those of DIAZEPAM that can cause ANTEROGRADE AMNESIA. Some reports indicate that it is used as a date rape drug and suggest that it may precipitate violent behavior. The United States Government has banned the importation of this drug.
The measurement of the density of a material by measuring the amount of light or radiation passing through (or absorbed by) the material.
Unstable isotopes of copper that decay or disintegrate emitting radiation. Cu atoms with atomic weights 58-62, 64, and 66-68 are radioactive copper isotopes.
An anticonvulsant that is the active metabolite of TRIMETHADIONE.
A butyrophenone with general properties similar to those of HALOPERIDOL. It has been used in the treatment of aberrant sexual behavior. (From Martindale, The Extra Pharmacopoeia, 30th ed, p567)
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A subfamily of G-PROTEIN-COUPLED RECEPTORS that bind the neurotransmitter DOPAMINE and modulate its effects. D2-class receptor genes contain INTRONS, and the receptors inhibit ADENYLYL CYCLASES.
The study of the chemical and physical phenomena of radioactive substances.
A highly selective and specific beta antagonist that is used to characterize beta-adrenoceptors.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Cell surface proteins that bind somatostatin and trigger intracellular changes which influence the behavior of cells. Somatostatin is a hypothalamic hormone, a pancreatic hormone, and a central and peripheral neurotransmitter. Activated somatostatin receptors on pituitary cells inhibit the release of growth hormone; those on endocrine and gastrointestinal cells regulate the absorption and utilization of nutrients; and those on neurons mediate somatostatin's role as a neurotransmitter.
Elements of limited time intervals, contributing to particular results or situations.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Cell surface proteins that bind ANGIOTENSINS and trigger intracellular changes influencing the behavior of cells.
Cell-surface proteins that bind dopamine with high affinity and trigger intracellular changes influencing the behavior of cells.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An amphetamine analog that is rapidly taken up by the lungs and from there redistributed primarily to the brain and liver. It is used in brain radionuclide scanning with I-123.
Organic or inorganic compounds that contain the -N3 group.
A spiro butyrophenone analog similar to HALOPERIDOL and other related compounds. It has been recommended in the treatment of SCHIZOPHRENIA.
Isotopes that exhibit radioactivity and undergo radioactive decay. (From Grant & Hackh's Chemical Dictionary, 5th ed & McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The circulation of blood through the BLOOD VESSELS of the BRAIN.
A moderately lipophilic beta blocker (ADRENERGIC BETA-ANTAGONISTS). It is non-cardioselective and has intrinsic sympathomimetic actions, but little membrane-stabilizing activity. (From Martindale, The Extra Pharmocopoeia, 30th ed, p638)
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A subclass of IMIDES with the general structure of pyrrolidinedione. They are prepared by the distillation of ammonium succinate. They are sweet-tasting compounds that are used as chemical intermediates and plant growth stimulants.
A cardioselective beta-1-adrenergic antagonist with no partial agonist activity.
Striped GRAY MATTER and WHITE MATTER consisting of the NEOSTRIATUM and paleostriatum (GLOBUS PALLIDUS). It is located in front of and lateral to the THALAMUS in each cerebral hemisphere. The gray substance is made up of the CAUDATE NUCLEUS and the lentiform nucleus (the latter consisting of the GLOBUS PALLIDUS and PUTAMEN). The WHITE MATTER is the INTERNAL CAPSULE.
Small computers that lack the speed, memory capacity, and instructional capability of the full-size computer but usually retain its programmable flexibility. They are larger, faster, and more flexible, powerful, and expensive than microcomputers.
A neurotoxic isoxazole isolated from species of AMANITA. It is obtained by decarboxylation of IBOTENIC ACID. Muscimol is a potent agonist of GABA-A RECEPTORS and is used mainly as an experimental tool in animal and tissue studies.
A nitroimidazole that sensitizes normally radio-resistant hypoxic cells to radiation. It may also be directly cytotoxic to hypoxic cells and has been proposed as an antineoplastic.
The first artificially produced element and a radioactive fission product of URANIUM. Technetium has the atomic symbol Tc, atomic number 43, and atomic weight 98.91. All technetium isotopes are radioactive. Technetium 99m (m=metastable) which is the decay product of Molybdenum 99, has a half-life of about 6 hours and is used diagnostically as a radioactive imaging agent. Technetium 99 which is a decay product of technetium 99m, has a half-life of 210,000 years.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Analogs or derivatives of AMPHETAMINE. Many are sympathomimetics and central nervous system stimulators causing excitation, vasopressin, bronchodilation, and to varying degrees, anorexia, analepsis, nasal decongestion, and some smooth muscle relaxation.
Unstable isotopes of phosphorus that decay or disintegrate emitting radiation. P atoms with atomic weights 28-34 except 31 are radioactive phosphorus isotopes.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Stable strontium atoms that have the same atomic number as the element strontium, but differ in the atomic weight. Sr-84, 86, 87, and 88 are the stable strontium isotopes.
Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.
Any diagnostic evaluation using radioactive (unstable) isotopes. This diagnosis includes many nuclear medicine procedures as well as radioimmunoassay tests.
An organization of cells into an organ-like structure. Organoids can be generated in culture. They are also found in certain neoplasms.
Specific sites or molecular structures on cell membranes or in cells with which phencyclidine reacts or to which it binds to elicit the specific response of the cell to phencyclidine. Studies have demonstrated the presence of multiple receptor sites for PCP. These are the PCP/sigma site, which binds both PCP and psychotomimetic opiates but not certain antipsychotics, and the PCP site, which selectively binds PCP analogs.
A high-affinity muscarinic antagonist commonly used as a tool in animal and tissue studies.
The process by which a DNA molecule is duplicated.
Cell surface proteins that bind VASOACTIVE INTESTINAL PEPTIDE; (VIP); with high affinity and trigger intracellular changes which influence the behavior of cells.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Tomography using radioactive emissions from injected RADIONUCLIDES and computer ALGORITHMS to reconstruct an image.
Cell membrane proteins that bind opioids and trigger intracellular changes which influence the behavior of cells. The endogenous ligands for opioid receptors in mammals include three families of peptides, the enkephalins, endorphins, and dynorphins. The receptor classes include mu, delta, and kappa receptors. Sigma receptors bind several psychoactive substances, including certain opioids, but their endogenous ligands are not known.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Volume of biological fluid completely cleared of drug metabolites as measured in unit time. Elimination occurs as a result of metabolic processes in the kidney, liver, saliva, sweat, intestine, heart, brain, or other site.
Compounds with BENZENE fused to AZEPINES.
The compound is given by intravenous injection to do POSITRON-EMISSION TOMOGRAPHY for the assessment of cerebral and myocardial glucose metabolism in various physiological or pathological states including stroke and myocardial ischemia. It is also employed for the detection of malignant tumors including those of the brain, liver, and thyroid gland. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1162)
The thin layer of GRAY MATTER on the surface of the CEREBRAL HEMISPHERES that develops from the TELENCEPHALON and folds into gyri and sulchi. It reaches its highest development in humans and is responsible for intellectual faculties and higher mental functions.
A subclass of G-protein coupled SEROTONIN receptors that couple preferentially to GI-GO G-PROTEINS resulting in decreased intracellular CYCLIC AMP levels.
Cell-surface proteins that bind SEROTONIN and trigger intracellular changes which influence the behavior of cells. Several types of serotonin receptors have been recognized which differ in their pharmacology, molecular biology, and mode of action.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Neurotoxic proteins from the venom of the banded or Formosan krait (Bungarus multicinctus, an elapid snake). alpha-Bungarotoxin blocks nicotinic acetylcholine receptors and has been used to isolate and study them; beta- and gamma-bungarotoxins act presynaptically causing acetylcholine release and depletion. Both alpha and beta forms have been characterized, the alpha being similar to the large, long or Type II neurotoxins from other elapid venoms.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A genus of aquatic newts in the Salamandridae family. During breeding season many Triturus males have a dorsal crest which also serves as an accessory respiratory organ. One of the common Triturus species is Triturus cristatus (crested newt).
A method of chemical analysis based on the detection of characteristic radionuclides following a nuclear bombardment. It is also known as radioactivity analysis. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An essential branched-chain amino acid important for hemoglobin formation.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The directed transport of ORGANELLES and molecules along nerve cell AXONS. Transport can be anterograde (from the cell body) or retrograde (toward the cell body). (Alberts et al., Molecular Biology of the Cell, 3d ed, pG3)
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A class of compounds of the type R-M, where a C atom is joined directly to any other element except H, C, N, O, F, Cl, Br, I, or At. (Grant & Hackh's Chemical Dictionary, 5th ed)
A family of G-protein-coupled receptors that are specific for and mediate the effects of MELATONIN. Activation of melatonin receptors has been associated with decreased intracellular CYCLIC AMP and increased hydrolysis of PHOSPHOINOSITIDES.

Ambiguity of the thymidine index. (1/6999)

The observed thymidine indices of seven experimental tumor lines are compared as a function of duration of emulsion exposure. The effects of dose level of tritiated thymidine and background threshold are also evaluated. The results indicate that an arbitrary high background threshold discriminates against "lightly" labeled cells at short periods of exposure but that the chosen threshold becomes less critical with longer exposure. The observed thymidine index increases with increasing duration of emulsion exposure but appears to approach a plateau for all tumor systems. The "thymidine index curves" are significantly different for each tumor. There is an inverse relationship between the dose of tritiated thymidine and the duration of exposure required to recognize the same fraction of cells as labeled in a given tumor. Similar experimental conditions do not necessarily guarantee a valid basis for comparison of observed thymidine indices among tumors.  (+info)

Integrin subunit gene expression is regionally differentiated in adult brain. (2/6999)

Integrins are a diverse family of heterodimeric (alphabeta) adhesion receptors recently shown to be concentrated within synapses and involved in the consolidation of long-term potentiation. Whether neuronal types or anatomical systems in the adult rat brain are coded by integrin type was studied in the present experiments by mapping the relative densities of mRNAs for nine alpha and four beta subunits. Expression patterns were markedly different and in some regions complementary. General results and areas of notable labeling were as follows: alpha1-limited neuronal expression, neocortical layer V, hippocampal CA3; alpha3 and alpha5-diffuse neuronal and glial labeling, Purkinje cells, hippocampal stratum pyramidale, locus coeruleus (alpha3); alpha4- discrete limbic regions, olfactory cortical layer II, hippocampal CA2; alpha6-most prominently neuronal, neocortical subplate, endopiriform, subiculum; alpha7-discrete, all neocortical layers, hippocampal granule cells and CA3, cerebellar granule and Purkinje cells, all efferent cranial nerve nuclei; alpha8-discrete neuronal, deep cortex, hippocampal CA1, basolateral amygdala, striatum; alphaV-all cortical layers, striatum, Purkinje cells; beta4-dentate gyrus granule cells; beta5-broadly distributed, neocortex, medial amygdala, cerebellar granule and Purkinje cells, efferent cranial nerve nuclei; alpha2, beta2, and beta3-mRNAs not detected. These results establish that brain subfields express different balances of integrin subunits and thus different integrin receptors. Such variations will determine which matrix proteins are recognized by neurons and the types of intraneuronal signaling generated by matrix binding. They also could generate important differences in synaptic plasticity across brain systems.  (+info)

Activated macrophages and microglia induce dopaminergic sprouting in the injured striatum and express brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. (3/6999)

Nigrostriatal dopaminergic neurons undergo sprouting around the margins of a striatal wound. The mechanism of this periwound sprouting has been unclear. In this study, we have examined the role played by the macrophage and microglial response that follows striatal injury. Macrophages and activated microglia quickly accumulate after injury and reach their greatest numbers in the first week. Subsequently, the number of both cell types declines rapidly in the first month and thereafter more slowly. Macrophage numbers eventually cease to decline, and a sizable group of these cells remains at the wound site and forms a long-term, highly activated resident population. This population of macrophages expresses increasing amounts of glial cell line-derived neurotrophic factor mRNA with time. Brain-derived neurotrophic factor mRNA is also expressed in and around the wound site. Production of this factor is by both activated microglia and, to a lesser extent, macrophages. The production of these potent dopaminergic neurotrophic factors occurs in a similar spatial distribution to sprouting dopaminergic fibers. Moreover, dopamine transporter-positive dopaminergic neurites can be seen growing toward and embracing hemosiderin-filled wound macrophages. The dopaminergic sprouting that accompanies striatal injury thus appears to result from neurotrophic factor secretion by activated macrophages and microglia at the wound site.  (+info)

Development and cytodifferentiation of the rabbit pars intermedia. II. Neonatal to adult. (4/6999)

Material from pars intermedia obtained from rabbits ranging from the second week post-partum to the adult stage, and including specimens from pregnant animals, was studied. The rate of cell division became greatly reduced early in postnatal) development. The commonest type of cell (the pars intermedia-glandular cell) becomes increasingly PAS-positive during the early stages of development. Although by 35 days differentiation of all the ACT-type cells is complete, the pars intermedia-glandular cells take as long as 53 days to mature. The epithelioid border of the hypophysial cleft persists throughout life, commonly containing dark cells. A ciliary fringe frequently appears in neonates and persists in pregnancy. Possible functions of such cilia are discussed. Throughout development the fine structure of the vasculature was studied. Secretory granules resembling those within the cells were seen in and around the blood vessels, and the mode of endocrine secretion in the pars intermedia tissue is discussed. The pars intermedia-glandular cells of the pregnant rabbits appeared hyperactive. The functional significance of the mammalian pars intermedia is discussed.  (+info)

Onset of nucleolar and extranucleolar transcription and expression of fibrillarin in macaque embryos developing in vitro. (5/6999)

Specific aims were to characterize the onset of nucleolar and extranucleolar transcription and expression of the nucleolar protein fibrillarin during preimplantation development in vitro in macaque embryos using autoradiographic and immunocytochemical techniques. Autoradiography was performed on whole embryos cultured with [3H]uridine for assessment of nucleolar (rRNA) and extranucleolar (mRNA) transcription. Expression of fibrillarin was immunocytochemically assessed in whole embryos using a primary antibody against fibrillarin and a fluorescein isothiocyanate-conjugated secondary antibody. Extranucleolar incorporation of [3H]uridine was first detected in 2-cell embryos cultured 6-10 h with [3H]uridine. Culture with alpha-amanitin prevented incorporation of label in 2-cell embryos, and treatment with ribonuclease reduced the signal to background levels, indicating that [3H]uridine was incorporated into mRNA and not rRNA or DNA. Nucleolar incorporation of [3H]uridine was not evident in pronucleate-stage or 2- to 5-cell embryos, but it was detected in one 6-cell embryo and in all 8-cell to blastocyst-stage embryos. Fibrillarin was first expressed in some 6- to 7-cell embryos, but it was consistently expressed in all 8-cell embryos. Fibrillarin was localized to the perimeter of the nucleolar precursor bodies, forming a ring that completely encapsulated these structures. Fibrillarin was not expressed in 8- to 16-cell embryos cultured with alpha-amanitin, indicating that it is transcribed, rather than recruited, at the 8-cell stage. In conclusion, in in vitro-fertilized macaque embryos developing in vitro, extranucleolar synthesis of mRNA is initiated at the 2-cell stage while the onset of nucleolar transcription occurs at the 6- to 8-cell stage, coincident with expression of fibrillarin.  (+info)

The cerebral metabolic consequences of nitric oxide synthase deficiency: glucose utilization in endothelial and neuronal nitric oxide synthase null mice. (6/6999)

Nitric oxide has multiple physiologic roles in the CNS. Inhibiting nitric oxide synthesis might therefore alter functional activity within the brain. We used [14C]-2-deoxyglucose in vivo autoradiography to measure local CMRglc in "knockout" mice lacking the genes for either the endothelial (eNOS) or neuronal (nNOS) isoforms of nitric oxide synthase, and in the progenitor strains (SV129, C57B1/6). Glucose utilization levels did not significantly differ between nNOS and eNOS knockout mice and C57B1/6 mice in any of the 48 brain regions examined, but were relatively lower in some subcortical regions in SV129 mice.  (+info)

Age-related reductions in [3H]WIN 35,428 binding to the dopamine transporter in nigrostriatal and mesolimbic brain regions of the fischer 344 rat. (7/6999)

In the present study, we used the potent cocaine analog [3H]WIN 35, 428 to map and quantify binding to the dopamine transporter (DAT) within the dorsal striatum, nucleus accumbens, substantia nigra, and ventral tegmental area in young (6-month-old), middle-aged (12-month-old), and aged (18- and 24-month-old) Fischer 344 rats. Quantitative autoradiographic analysis of indirect [3H]WIN 35,428 saturation curves revealed two-site binding for all four brain regions in every age group. The percentage of binding to the high- or low-affinity sites did not differ with age or region and was approximately 50%. However, significant age-related decreases in the overall density (Bmax) of [3H]WIN 35,428-binding sites were observed in the striatum, nucleus accumbens, substantia nigra, and ventral tegmental area. The Bmax within all brain regions declined by more than 15% every 6 months, with the Bmax in the aged (24-month-old) group being approximately half that measured in the young adult (6-month-old) group. Competition experiments indicated that nomifensine also exhibited two-site binding to the DAT in Fischer 344 rats. No consistent age-related differences in binding affinities were noted with either [3H]WIN 35,428 or nomifensine. Taken together, these results support the hypothesis that functional DATs within the nigrostriatal and mesolimbic systems are down-regulated with age, without changing their affinity for ligands.  (+info)

Effect of diabetes and aminoguanidine therapy on renal advanced glycation end-product binding. (8/6999)

BACKGROUND: Advanced glycation end-products (AGEs) have been implicated in the pathogenesis of diabetic nephropathy, and aminoguanidine (AG) has been shown to decrease the accumulation of AGEs in the diabetic kidney. METHODS: This study investigates changes in AGE binding associated with diabetes in the rat kidney using in vitro and in vivo autoradiographic techniques. Male Sprague-Dawley rats were randomized into control and diabetic groups with and without AG treatment and were sacrificed after three weeks. Frozen kidney sections (20 microm) were incubated with [125I]-AGE-RNase or [125I]-AGE-BSA. To localize the AGE binding site, in vivo autoradiography was performed by injection of 15 microCi of [125I]-AGE-BSA into the abdominal aorta of the rat. RESULTS: Low-affinity binding sites specific for AGEs in the renal cortex (IC50 = 0.28 microm) were detected by in vitro autoradiography. There was a significant increase in [125I]-AGE binding in the diabetic kidney, which was prevented by AG treatment. Emulsion autoradiography revealed that binding was localized primarily to proximal tubules in the renal cortex. Renal AGE levels, as assessed by fluorescence or by radioimmunoassay, were increased after three weeks of diabetes. This increase was attenuated by AG therapy. CONCLUSIONS: AGE binding sites are present within the proximal tubules of the kidney and appear to be modulated by endogenous AGE levels. It remains to be determined if these binding sites represent receptors involved in clearance of AGEs or are linked to pathogenic pathways that lead to the development of diabetic nephropathy.  (+info)

After monolayer cultures of rat islets were exposed to [125I]insulin,[125I]glucagon, and [125I]tyrosinyl somatostatin, specific autoradiographic grains associated with each radioactively labeled ligand were found on B, A, and D cells.The density of labeling of the B, A, and D cells with each labeled ligand correlated well with the known actions of the three hormones on each of the islet cells. ...
Many physiological mechanisms as well as numerous neurological diseases involve the complex interaction of various receptors. Quantitative evaluation of the receptor distribution is essential for developing improved procedures in diagnosis and effective treatments of neurological diseases.. Quantitative Receptor Autoradiography. Using in-vitro autoradiography we analyze a broad range of different neuroreceptors in brain slices (10 -20 µm thick). After preincubation in buffer to remove endogenous ligand, sections are incubated with a radioactive substance, that binds specificly to the receptor being studied (radioligand). For the assessment of non-specific binding, selected slices are incubated with the radioligand in presence of an specific antagonist. After washing and a rapid rinse in ice-cold water the sections are dried and exposed against phosphor-imaging plates or conventional film slides in combination with tritium activity standards (see figure 1) for quantitative evaluation. Specific ...
An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance. Alternatively, the autoradiograph is also available as a digital image (digital autoradiography), due to the recent development of scintillation gas detectors or rare earth phosphorimaging systems. The film or emulsion is apposed to the labeled tissue section to obtain the autoradiograph (also called an autoradiogram). The auto- prefix indicates that the radioactive substance is within the sample, as distinguished from the case of historadiography or microradiography, in which the sample is X-rayed using an external source. Some autoradiographs can be examined microscopically for localization of silver grains (such as on the interiors or exteriors of cells or organelles) in which the process is termed micro-autoradiography. For example, micro-autoradiography was used to examine whether atrazine was being ...
TY - JOUR. T1 - Tritiated 2‐deoxy‐D‐glucose. T2 - A high‐resolution marker for autoradiographic localization of brain metabolism. AU - Hammer, Ronald P.. AU - Herkenham, Miles. PY - 1984/1/1. Y1 - 1984/1/1. N2 - The technique for autoradiographic localization of 2‐deoxy‐D‐glucose (2DG) uptake has become a useful method for observing alterations of functional brain activity resulting from experimental manipulation. Autoradiographic resolution is improved using tritiated ([3H]) rather than carbon‐14 ([14C])2DG, due to the lower energy and shorter path of tritium emissions. In addition, lower 2DG uptake by white matter relative to gray matter is exaggerated in the [3H]2DG autoradiographs due to the greater absorption of tritium emissions by lipids. Using [3H]2DG, it is possible to observe differential metabolic labeling in various individual nuclei or portions of nuclei that is unresolvable using [14C]2DG in the awake, normal animal. Heterogeneous patterns of 2DG uptake seen only ...
Various techniques for detection of blood platelets in tissue section were evaluated in an incremental air embolism model of ischemia in order to further investigate the accumulation of platelets in brains subjected to cell-damaging ischemia. The experimental animals were dogs anesthetized with alpha-chloralose. A new autoradiographic technique was devised that allows precise localization of 111Indium-labeled platelets in brain sections. The technique is described in detail along with some effects of the label on platelet function and behavior. The technique can be performed in conjunction with 14C-iodoantipyrine autoradiographic measurement of blood flow without causing mutual interference or other forms of interaction. This permits the simultaneous investigation of local blood flow and deposition of platelets. ...
Double-tracer autoradiographic study of protein synthesis and glucose consumption in rats with focal cerebral ischemia. - T Christensen, T Balchen, T Bruhn, N H Diemer
A potent, synthetic cannabinoid was radiolabeled and used to characterize and precisely localize cannabinoid receptors in slide- mounted sections of rat brain and pituitary. Assay conditions for 3H- CP55,940 binding in Tris-HCl buffer with 5% BSA were optimized, association and dissociation rate constants determined, and the equilibrium dissociation constant (Kd) calculated (21 nM by liquid scintillation counting, 5.2 nM by quantitative autoradiography). The results of competition studies, using several synthetic cannabinoids, add to prior data showing enantioselectivity of binding and correlation of in vitro potencies with potencies in biological assays of cannabinoid actions. Inhibition of binding by guanine nucleotides was selective and profound: Nonhydrolyzable analogs of GTP and GDP inhibited binding by greater than 90%, and GMP and the nonhydrolyzable ATP analog showed no inhibition. Autoradiography showed great heterogeneity of binding in patterns of labeling that closely conform to ...
We used the highly selective 5-HT(6) receptor radioligand [(125)I]SB-258585 (4-iodo-N-[4-methoxy-3-(4-methylpiperazin-1-yl)phenyl]benzene-sulfonamide) to perform autoradiographic binding studies on the rat brain. High levels of specific binding occurred in the corpus striatum, nucleus accumbens, Islands of Calleja and the olfactory tubercle. A high level of binding also appeared in the choroid plexus. Moderate levels occurred in several regions of the hippocampal formation and in certain regions of the cerebral cortex, thalamus, hypothalamus, and substantia nigra; and very low levels in the globus pallidus, cerebellum, other mesencephalic regions, and the rhombencephalon. Displacement of total binding with 10 microM unlabelled SB-214111 (4-bromo-N-[4-methoxy-3-(4-methylpiperazin-1-yl)phenyl]benzene-sulfonamide), another selective 5-HT(6) receptor antagonist, or 10 microM unlabelled methiothepin, reduced binding to barely discernible levels. Some animals received unilateral injections of 6
We have investigated 5-HT1A (serotonin1A) and 5-HT2A (serotonin2A) receptor mRNA abundance and binding site densities in various neocortical and hippocampal regions of schizophrenics and control subjects. Age, agonal state (brain pH), and post mortem interval were included where necessary as covariates in our analyses. In schizophrenics, 5-HT1A binding site densities, determined autoradiographically by [3H]8-hydroxy-2,3-(dipropylamino)-tetralin ([3H]8-OH-DPAT), were significantly increased (+23%) in the dorsolateral prefrontal cortex, with a similar trend in anterior cingulate gyrus. These increases were not accompanied by any change in 5-HT1A receptor mRNA. No differences between the groups in [3H]8-OH-DPAT binding or 5-HT1A receptor mRNA were seen in superior temporal gyrus, striate cortex, or hippocampus. 5-HT2A binding sites, determined by [3H]ketanserin, were decreased in the dorsolateral prefrontal cortex (-27%) and parahippocampal gyrus (-38%) of schizophrenics, with a similar trend in cingulate
Blanks, J C. and Bok, D, An autoradiographic analysis of postnatal cell proliferation in the normal and degenerative mouse retina. (1977). Subject Strain Bibliography 1977. 3447 ...
Mendelsohn, M L., Autoradiographic analysis of cell proliferation in spontaneous breast cancer of c3h mouse. Iii. The growth factor. (1961). Subject Strain Bibliography 1961. 685 ...
Unmodified oligodeoxynucleotides (ODNs) were synthesized and tested for their ability to cross external eukaryotic cell membranes and to enter the cytosol and nucleus in tissue cultures. The ODNs were labeled with high-specific-activity [3H]thymidine (| or = 100 Ci/mmol), or [ alpha-32P]ATP or [ gamma-32P]ATP (300-1000 Ci/mmol; 1 Ci = 37 GBq), and the label was either in the central portion of the molecule or at the 3 or 5 end. The cells employed were for the most part 3T6 murine fibroblasts, grown in monolayers, either semiconfluent or confluent, but some experiments were carried out with chicken embryo fibroblasts or human HeLa cells. Parallel wells in the same experiment were prepared for electron microscopy or for cell fractionation and radioactivity assays. Electron microscopic autoradiography indicated that ODNs cross the external cell membrane, traverse the cytosol, and begin to enter the cell nucleus within a few seconds to 5 min at 37 degrees C in Dulbeccos medium without added serum. After
CCD (charged coupled device) and CMOS imaging technologies can be applied to thin tissue autoradiography as potential imaging alternatives to using conventional film. In this work, we compare two particular devices: a CCD operating in slow scan mode and a CMOS-based active pixel sensor, operating at near video rates. Both imaging sensors have been operated at room temperature using direct irradiation with images produced from calibrated microscales and radiolabelled tissue samples. We also compare these digital image sensor technologies with the use of conventional film. We show comparative results obtained with (14)C calibrated microscales and (35)S radiolabelled tissue sections. We also present the first results of (3)H images produced under direct irradiation of a CCD sensor operating at room temperature. Compared to film, silicon-based imaging technologies exhibit enhanced sensitivity, dynamic range and linearity.. ...
Regional measurements of 131I-9.2.27 distribution in human melanoma tumors were obtained using quantitative autoradiography. Tumors were removed from patients 72-96 h after they had received an i.v. injection of 9.15 mCi (100 mg) of 131I-9.2.27. The autoradiographic images showed that the radioactivity reaching the tumor was heterogeneously distributed. Areas of relative high and low uptake were selected in each tumor. Regions of high activity contained from 51 to 1371 nCi/g, while areas with low uptake had radioactivity ranging from 12 to 487 nCi/g. The reliability of the autoradiographic measurements was demonstrated by the strong positive correlation with direct tissue sample counting (r = 0.994 P , 0.001). Since comparative immunocytochemistry showed a homogeneous and diffuse staining of target antigen on viable tumor cells, variability of monoclonal antibody uptake within individual tumors was not primarily due to heterogeneity of antigen expression in these cases. However, antigen levels ...
The presence and abundance of 5-HT1A and 5-HT2A receptor mRNAs in post mortem human hippocampus was investigated using a novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) technique using cyclophilin mRNA as an internal standard. 5-HT1A and 5-HT2A receptor mRNAs were each co-amplified with varying dilutions of cyclophilin primers, and their abundance expressed as a ratio of cyclophilin mRNA. Using this technique in combination with quantitative autoradiography we have investigated the effect of aging on hippocampal 5-HT1A and 5-HT2A receptor mRNA abundance and binding site densities. There was a significant negative correlation between hippocampal 5-HT1A receptor binding site densities and age and a similar trend for 5-HT1A receptor mRNA abundance. Neither 5-HT2A receptor binding site densities nor mRNA abundance were affected by age. Both 5-HT1A and 5-HT2A receptor binding site densities in individual subjects correlated significantly with abundance of their encoding mRNA. This
Read Quantitative autoradiographic mapping of 5‐HT 3 receptors in the rat CNS using ( 125 I)iodo‐zacopride and ( 3 H)zacopride as radioligands, Synapse on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Distribution of 2-chloro-2 -deoxyadenosine, 2-chloro-2 -arabino-fluoro-2 -deoxyadenosine, Fludarabine and Cytarabine in mice: a whole-body autoradiography study ...
Ling, E.A. (1974). Ultrastructure and radioautographic studies of the thyroid gland. Annals of the Academy of Medicine Singapore 3 (3) : 207-211. [email protected] Repository ...
A technique has been developed that allows repeated autoradiographs to be made of the isotope distribution in the chromosomes of a single cell. A series of 10 separate autoradiographs were made of a Chinese hamster diploid male metaphase cell which had been labeled with tritiated thymidine during the first 15 minutes of its DNA synthesis period in the previous interphase. Each autoradiograph had low grain densities above the chromosomes so that quantitation was feasible. The separate autoradiographs were photographically combined into a single composite in which grain images were converted to lines oriented at right angles to the chromosome axis. The line densities were then measured with a recording microdensitometer to yield graphs reflecting the isotope distribution along each chromosome. The area under each graph was directly proportional to the total number of grains counted above the corresponding chromosome in the 10 separate autoradiographs. The distribution of isotope along the ...
Morphological analysis of transplantable pancreatic acinar carcinoma of rat has revealed a continuum of cells from those which totally lack mature secretory granules to cells with abundant well-formed secretory granules. This tumor is easily dissociable into single cells which incorporate [3H]thymidine and [3H]leucine into DNA and protein at a linear rate for 3 to 4 hr. The number of cells synthesizing DNA, as assessed by light microscope autoradiography, was about 17%. Dissociated neoplastic pancreatic acinar cells are classified into four cell types (types I to IV) on the basis of nuclear morphology, degree of polarization, and the extent of specialization of cytoplasmic organelles. Type IV cells possess all the characteristics of mature pancreatic acinar cells, such as abundant rough endoplasmic reticulum, prominent Golgi apparatus, and numerous secretory granules. Cell types III, II, and I are progressively less differentiated. An analysis of the cytological features of 285 dissociated tumor ...
Agar Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions suitable for Autoradiography studies and the detection of radioactively labelled samples.
The distribution of infused tritiated norepinephrine (NE-3H) in small mesenteric arteries and intestinal arterioles in rats was investigated with electron microscopic radioautography. Silver grains, indicating the presence of the tritium label on the sections, were found lying mainly over axon bundles, but some were present over collagen and smooth muscle cells. Axons with the highest concentrations of silver grains had been sectioned at points where they were naked of Schwann cell sheath, were dilated into varicosities, and contained small granular vesicles. This finding was taken as confirmatory circumstantial evidence that the small granular vesicles were the sites of uptake and storage of NE. The short interval between the start of infusion and the fixation of the tissue appeared to rule out any process other than a direct uptake of NE by the peripheral axons. If axonal sites of uptake of NE-3H correspond to sites of release of NE, then the evidence suggests that such sites of release are ...
The Center for Quantitative Cancer Imaging has as part of its basic science imaging infrastructure a Fuji BAS-5000 Phosphor imager. This device offers extremely high resolution (25 μm) and image quality. It uses Fujifilms unique confocal laser and light-collecting optics. The BAS-5000 is amenable to fine-structure studies of a wide variety of tissue samples. With a dynamic range up to five orders of magnitude and a pixel size as small as 25 μm, the system allows for very high-resolution quantitative autoradiography studies. The system can provide rapid scan times. A 20 x 25 cm imaging plate can be scanned at 50 μm in as little as five minutes. The system includes software for region of interest (ROI) analysis and quantitative assessment of tracer accumulation. The system can image all PET isotopes as well as C-14 and tritium. The system also has the capabilities to perform various optical-based assays, including 2D electrophoresis and thin layer chromatography.. ...
Conceptual computer illustration of the DNA double helix together with a graphic representation of an autoradiograph display. The pattern of the DNA autoradiograph bands is unique to each individual, but some bands are shared by related people, such as a parent & child. DNA fingerprints can be used to prove conclusively whether people are related. - Stock Image C010/5265
The PMGKAY was announced in 2020 for three months till July and later extended till November to combat the economic impact of COVID-19 on the poor
The distribution of cells expressing mu-receptor mRNA and mu-receptor binding sites were compared in brain and spinal cord tissue sections using a combination of in situ hybridization and receptor autoradiographic techniques. mu-Receptor mRNA was visualized with a 35S-labeled cRNA probe directed to transmembrane III-VI of the rat mu-receptor, while mu-receptor binding sites were labeled with the mu-selective ligand [3H]DAMGO ...
TY - JOUR. T1 - Prediction of histopathological outcome using averaged multimodal information in rat. AU - Zhao, Weizhao. AU - Ginsberg, Myron D.. AU - Belayev, Ludmila. AU - Truettner, Jessie. AU - Schmidt-Kastner, Rainald. PY - 1998/12/1. Y1 - 1998/12/1. N2 - Commonly used autoradiographic image analysis for stroke research has been restricted to the assessment of local cerebral blood flow (LCBF), glucose utilization (LCMRglc) or messenger RNA (mRNA) (by in situ hybridization) in individual brains, with repeated measurements from the same animal. Histopathological analysis of perfusion-fixed paraffin-embedded brain material has long been regarded as the `gold standard for the quantitative assessment of tissue injury in experimental models of cerebral ischemia. It is desirable to reveal the interrelationship between signals measured at the time of ischemia and the final distribution of cell damage. In this study, averaged images for each modality were derived from individual rats and ...
The IVISR provides optical, CT, ultrasound, high-resolution planar gamma imaging, and PET of mice and rats, as well as high-resolution digital autoradiography and fluorescent imaging of thin whole body rodent sections.
Biospace Lab was founded in 1989 by G. Charpak to industrialize the world first digital autoradiography system and distribute it globally. He was awarded by
The autoradiographic analysis of neurotransmitter receptor distribution is a powerful technique that provides extensive information on the localization of neurotransmitter systems. Computer methodologies are described for the analysis of autoradiographic material which include quench correction, 3-dimensional display, and quantification based on anatomical boundaries determined from the tissue sections. These methodologies are applied to the problem of the distribution of glycine receptors measured by 3H-strychnine binding in the mouse CNS. The most distinctive feature of this distribution is its marked caudorostral gradient. The highest densities of binding sites within this gradient were seen in somatic motor and sensory areas; high densities of binding were seen in branchial efferent and special sensory areas. Moderate levels were seen in nuclei related to visceral function. Densities within the reticular formation paralleled the overall gradient with high to moderate levels of binding. The ...
Figure 1 Antiphospho-Pak1 antibody specifically recognizes activated Pak1. (A) Activation loop sequence of the phosphorylated peptide used for production of polyclonal antiphospho-Pak1 antisera. (B) Immunoblots (lanes 1-4) and radiolabeled kinase reaction products (lanes 5 and 6) of baculovirus-produced Pak1. Purified KD or CA Pak1 was incubated in protein kinase buffer in the presence of ATP, then immunoblotted using anti-Pak1 (lanes 1 and 2) and antiphospho-Pak antisera (lanes 3 and 4) autoradiographed to detect antibody-bound proteins. Although both forms of the protein were detected with anti-Pak1, only activated Pak1 was detected using the phospho-specific antibody, despite the large amount of recombinant protein used in this assay. Lanes 5 and 6 show autoradiographed PAGE gel of reaction products from a kinase assay performed in the presence of [γ-32P]ATP. Molecular mass in kilodaltons is shown to the right of the blot. (C) Comparison between immunoblot and radiolabeled kinase reaction ...
The first concerns cellular mechanisms of learning and memory. There is considerable evidence that a crucial step in memory formation is the synthesis of new proteins in the hippocampus. We are using autoradiographic techniques to focus on proteins that are made in dendrites of hippocampal neurons, near synapses. Very few proteins are made there, but we have found out that electrical stimulation of the axons that make contact with these dendrites greatly stimulates their synthesis of proteins. This only happens when the stimulus is combined with another neurotransmitter, acetylcholine. There is reason to believe this phenomenon is important in memory formation and we are studying its mechanisms and significance. The second area concerns the regulation of synaptic transmission by glycolysis. We have found that synaptic transmission in the hippocampus will only occur if glycolysis is active, even if adequate energy is supplied in other ways. This is very unexpected. There appears to be a ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Ive had a decades-long fascination with herbs and natural healing and found over the years that many such treatments can be quite successful. Im...
The Ghana Socioeconomic Panel Survey 2009-2010 collected baseline information for a panel of 5,009 households (18,889 household members) across all regions of Ghana. Two questionnaires collected information about household demographics, housing characteristics, household and agricultural assets, agricultural production, household enterprises, health and fertility, and household consumption and expenditure. Cognitive tests were conducted for children in the household, and psychological tests were conducted for the household head, spouse, and a randomly chosen household member.. A community questionnaire was also used to collect information about community institutions, political organizations, development programs, and infrastructure. Geographic (GPS) information was also recorded for all households and community facilities.. ...
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We investigated the effect of a novel quinazoline derivative (KB-5666), a lipid peroxidation inhibitor, on ischemic neuronal damage using Mongolian gerbils. The animals were sacrificed 7 or 30 days after 5 min of forebrain ischemia induced by bilateral common carotid artery occlusion. Morphologic changes, a microtubule-associated protein 2 (MAP2) immunohistochemical study and quantitative autoradiographic study using [3H]phorbol 12, 13-dibutyrate ([3H]PDBu) were evaluated in the hippocampus after ischemia. KB-5666 (3-50 mg/kg, i.v.) showed protective effects against neuronal death of the CA1 subfield 5 min before ischemia, immediately or 1 hr after ischemia, but not 4 hr after ischemia. KB-5666 (i.p.) also showed protective effects in a dose-dependent manner immediately after ischemia. Furthermore, KB-5666 dose-dependently prevented a marked decrease in microtubule-associated protein 2 immunoreactivity in the dendritic fields of the CA1 pyramidal cells after ischemia. The [3H]PDBu binding ...
The intracellular transport of venom proteins has been studied in active and resting venom glands of the snake Vipera palaestinae by electron microscope radioautography after an intra-arterial injection of [3H]leucine. In the active gland, most of the label is initially (10 min) found over the RER. By 30 min, the relative grain density of the Golgi complex reaches its maximum, with concomitant increase in the labeling of the condensing vacuoles. Later on, a steep increase in radioactivity of the secretory granules is observed. At 3 h, these granules, which comprise about 2% of the cell volume, contain 22% of the total grains. At the following hour, their labeling declines and at the same time the radioactivity of the secreted venom is increased. It is concluded that, in the active cell, venom proteins are transported via the Golgi apparatus into membrane-bounded granules which are the immediate source of the secreted venom. An alternative pathway, which involves the RER cisternae as a storage ...
Grain counting by eye is a tedious and time-consuming technique but one with great potential in cell kinetics and for the study of DNA excision repair activity (unscheduled DNA synthesis or UDS). We have been investigating the levels of UDS in human skin sections exposed in situ to ultraviolet radiation using a short-term incubation in tritiated thymidine and autoradiography and the decline in UDS levels with time (repair kinetics). We have adapted an automated image analysis system automatically to assess the number of grains over epidermal cell nuclei in autoradiographs of sections of epidermis. An excellent correlation was observed between visual counting and machine measurement of the area (in pixels) occupied by silver grains. The levels of UDS declined with time as lesions are progressively repaired. The half time (+/- standard deviation) for the reduction in UDS is 7.25 +/- 0.18 h. The grain counts can be significantly increased by increasing the autoradiographic exposure, by increasing ...
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The publisher regrets that a mistake was published in this article. On page 747, the word antagonist was incorrectly published instead of agonist. The whole sentence should correctly read: Importantly, also in response to an adenosine A1 agonist, old rats had less sleep compared with young animals. Our sincere apologies are offered to the authors, editor, and readers.. The original article of this erratum was published in J Neurosci Res 2004; 78: 742-748 ...
Charles River designs and conducts GLP-compliant quantitative whole-body autoradiography (QWBA) programs integrated with our preclinical drug metabolism services.
An assemblage useful for autoradiography comprising, in order, a sample layer containing a radioactively labeled biological sample in contact with a phosphor layer, a film layer and a reflector layer. The phosphor layer is preferably a gadolinium oxysulfide containing layer and the film is preferably a photographic film containing a tabular grain emulsion.
TY - JOUR. T1 - Autoradiography of monoamine uptake in Molluscan ganglia. AU - Elekes, K.. PY - 1978/1/1. Y1 - 1978/1/1. UR - http://www.scopus.com/inward/record.url?scp=0018084061&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0018084061&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0018084061. VL - 10. SP - S268. JO - Neuroscience Letters. JF - Neuroscience Letters. SN - 0304-3940. IS - suppl. 1. ER - ...
Read independent reviews on EN3HANCE™ Spray Surface Autoradiography Enhancer, 2oz Spray Can from PerkinElmer, Inc. on SelectScience
DNA. Computer artwork of a DNA (deoxyribonucleic acid) double helix seen against autoradiograms of genetic sequences (upper right). The spiralling strands of the DNA helix (blue) are composed of complex chemical groups called nucleotides, which consist of a sugar phosphate and a base group. Complementary pairing between the base groups of nucleotides on opposite strands holds the helix together. The sequence of the 4 base groups (adenine, cytosine, guanine and thymine; coloured rods) along the DNA helix is unique for every individual and is known as the genetic code. The autoradiograms depict DNA base sequences, each band (dark blue) representing an individual base. - Stock Image G110/0642
The effect of aerosol upon cloud properties is studied over the Baltic Sea region, which presents a distinct contrast of aerosol loading between the clean Fennoscandia and the polluted area of central-eastern Europe. Statistically significant positive values are found over the Baltic Sea and Fennoscandia, while negative values are found over central-eastern Europe, contradicting the theory of aerosol indirect effect on clouds ...
A variety of methods have been used in published studies for image segmentation (manual outlining, thresholding, magic wand, use of templates, etc.) to determine what is part of the structure or region of interest and what is not. Similarly, once grey levels are measured, a variety of methods have been employed to convert them into optical densities or units of radioactivity. Based on the above results, the line method was the best way to select the structure or region of interest as it was not subject to floor-effects, had a low coefficient of variation, and low within-sample variability. This method is similar to, and a modification of the outline method, which requires that the actual boundaries of the structure be determined, whereas the line method sampled only from the interior of the structure. Since the DG is a long narrow structure, this was best done with a line down the centre. Larger structures can follow the same principle by outlining the structure but staying well inside the ...
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There are 13 fifteen-letter words containing A, D, G, U and Y: AUTORADIOGRAPHY DAGUERREOTYPERS DAGUERREOTYPIES ... PSEUDOPREGNANCY RADIOAUTOGRAPHY UNDERSTANDINGLY. Every word on this site can be used while playing scrabble. Create other lists, beginning with or ending with letters of your choice.
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... Labsupply labsupply at aol.com Tue May 5 23:09:59 EST 1998 *Previous message: Practical Ion Channel ... labscientific, inc sells inexpensive autoradiography film suitable for ecl, western blots, sequencing and gel shift analysis, ...
Recommended for direct autoradiography of weak and medium energy β-radioisotopes, such as 35S,33P, and 14C ... whether from chemiluminescent Western blot or DNA or RNA autoradiography. ...
... Samuel C. Blackman scb1 at midway.uchicago.edu Fri May 16 15:22:51 EST 1997 *Previous ... transfer to nitrocellulose or PVDF and then detect the labelled proteins by autoradiography. Ive heard that it is possible if ...
An assemblage useful for autoradiography comprising, in order, a sample layer containing a radioactively labeled biological ... 1995 , entitled AUTORADIOGRAPHY ASSEMBLAGE USING TRANSPARENT SCREEN.. FIELD OF THE INVENTION. The present invention is directed ... This field is sometimes referred to as autoradiography.. BACKGROUND OF THE INVENTION. An autoradiograph is a recording of the ... Electronic imaging system for autoradiography. US6521329 *. 18 Jun 2001. 18 Feb 2003. Eastman Kodak Company. Radiographic ...
... to produce a radioactive rim that was imaged by autoradiography. The tagged salt was compacted at pressures up to 138 MPa /20 ... to produce a radioactive rim that was imaged by autoradiography. The tagged salt was compacted at pressures up to 138 MPa /20 ...
Quantitative autoradiography of serotonin receptors in the rat brain. Brain Res. 242: 197-204.PubMedCrossRefGoogle Scholar ... Serotonin receptor localization in rat brain by light microscopic autoradiography. Eur. J. Pharmacol. 62: 237-239.PubMed ... Receptor autoradiography with tritium-sensitive film: potential for computerized densitometry. Neuroscience Lett. 25: 101-105. ... Estradiol-concentrating neurons: Topography in the hypothalamus by dry-mount autoradiography. Science 162: 1001-1003.PubMed ...
This overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial ... Advances in autoradiography: A new film brings quality improvements. In The Biotechnology Report pp. 147‐149. Campden ... Quantitative autoradiography is described as a means of calibrating experimental results. Importantly, specific guidelines are ... This overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial ...
Autoradiography Accessories. BioMax Cassette. Designed to maximize image resolution and background clarity. ... Where to buy BioMax Autoradiography Film.. Find a Carestream Scientific Film Dealer in your area. ... The most sensitive intensifying screen available for use in autoradiography.. BioMax TranScreen HE. Provides maximum ...
AUTORADIOGRAPHY FILMS. Carestream Health, the worldwide leader in developing autoradiography films, sets the standard for ... A high-performance, all-purpose autoradiography film that provides maximum sensitivity. BioMax XAR A general purpose ... Autoradiography Film Tips Carestream Molecular Imaging recommended film storage, handling, proper safelighting and exposure. ... Where to buy BioMax Autoradiography Film.. Find a Carestream Scientific Film Dealer in your area. ...
Animal cell · Animal model · Gene rearrangement · Mouse · Nonhuman · Oncogene c myc · Animal · Autoradiography · Bone Marrow · ... Health · agar gel electrophoresis · autoradiography · blood sampling · cohort analysis · dna determination · genetic model · ... Nutrition · Radioisotope · Tin chloride · Animal experiment · Autoradiography · Blood and hemopoietic system · Electron ... Autoradiography · Bacterial Toxins · Carbon Radioisotopes · Cell Adhesion Molecules · Cells, Cultured · Cytotoxins · E-Selectin ...
Quantitative Receptor Autoradiography. Using in-vitro autoradiography we analyze a broad range of different neuroreceptors in ... In vitro techniques: Autoradiography, Immunohistochemistry, Mass spectrometry. Many physiological mechanisms as well as ... 2: Definition of regions in autoradiography: via histological stainings (right) it is possible to quantify the receptor density ...
Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions suitable for Autoradiography ... Ilford emulsions for autoradiography. Add to Order. Add to Quote. More Information Ilford offers nuclear emulsions with either ... Micro autoradiography. Agar Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions ... Kodak NTB emulsion for autoradiography. Add to Order. Add to Quote. More Information Kodak manufactures nuclear emulsion type ...
Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions suitable for Autoradiography ... Ilford emulsions for autoradiography. Add to Quote. More Information Ilford offers nuclear emulsions with either 0.20µm (K) or ... Micro autoradiography. Agar Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions ... Kodak NTB emulsion for autoradiography. Add to Quote. More Information Kodak manufactures nuclear emulsion type NTB for use in ...
... measured ex vivo by quantitative autoradiography.. Schotte A1, Janssen PF, Megens AA, Leysen JE. ... were investigated precisely using receptor autoradiography in sections of the same rat brain except for histamine H1 receptors ...
Kd values, determined from slice autoradiography, were between 9.17 and 15.6 nM. The HDAC1-3 density (Bmax), averaged over ... "Class I HDAC Imaging Using [3H]CI-994 Autoradiography." Epigenetics 8 (7) (July 1): 756-764. doi:10.4161/epi.25202. ... was evaluated as an autoradiography probe for ex vivo labeling and localizing of class I HDAC (isoforms 1-3) in the rodent ... we have developed the first known autoradiography tool for imaging class I HDAC enzymes. Although validated in the CNS, [3H]CI- ...
Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets ... Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets ... Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets ... Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets ...
Scientists studying a DNA sequencing autoradiogram of novel recombinant proteins cloned for the production of VaxSyn, an experimental AIDS vaccine. VaxSyn is based on a genetically- engineered, recombinant form of the HIV protein gp160, a precursor to one of HIVs surface proteins. VaxSyn contains no attenuated infectious parts of HIV & therefore carries no risk of transmitting AIDS. The vaccine is being developed by MicroGeneSys and is undergoing trials as a treatment of HIV-positive patients & as a potential vaccine against HIV infection. - Stock Image G210/0383
Autoradiography with Tritiated Methotrexate and the Cellular Distribution of Folate Reductase Message Subject. (Your Name) has ... Autoradiography with Tritiated Methotrexate and the Cellular Distribution of Folate Reductase. By Zbigniew Darzynkiewicz, ... Autoradiography with Tritiated Methotrexate and the Cellular Distribution of Folate Reductase. By Zbigniew Darzynkiewicz, ...
High Sensitivity Autoradiography Film, HyBlot CL® Autoradiography Film, BluLite™ Autoradiography.. ... found in: HyBlot CL® Autoradiography Film, HyBlot ES® ... HyBlot CL® Autoradiography Film labForce (a Thomas Scientific ... Extra sensitive autoradiography film, x-ray film For blotting & sequence autoradiography Use with 14C, 35S, 32P, 33P and 125I ... Also suitable for autoradiography, but with less sensitivity for high-energy isotopes than X-Omat AR and BioMax MS, and than ...
... ... imaging and quantitative whole-body autoradiography (QWBAR) with [18F]FDOPA. The images were applied for the measurement of ...
... of potential precursor populations in the mouse olfactory epithelium using immunocytochemistry and autoradiography. M Schwartz ... receptor cell regeneration stimulated by olfactory bulbectomy using a combination of immunocytochemistry and autoradiography. ... of potential precursor populations in the mouse olfactory epithelium using immunocytochemistry and autoradiography ... of potential precursor populations in the mouse olfactory epithelium using immunocytochemistry and autoradiography ...
A photographic emulsion is placed... Explanation of Autoradiography ... Find out information about Autoradiography. A photographic technique used to localize a radioactive substance within a solid ... Autoradiography. Also found in: Dictionary, Thesaurus, Medical, Wikipedia. Autoradiography. A photographic technique used to ... autoradiography. [¦ȯd·ō‚rād·ē′äg·rə·fē] (engineering) A technique for detecting radioactivity in a specimen by producing an ...
Microcomputer programs for quantitative autoradiography and stereology. Pierre Cau, Laboratoire dHistologie (ERA-CNRS n° 322 ... Pierre Cau & Jean-Louis Boudier, «Microcomputer programs for quantitative autoradiography and stereology», Acta Stereologica [ ... 4 BASIC programs for quantitative autoradiography and stereology were written for a Hewlett-Packard HP 85 microcomputer. ...
The anatomical distribution of the radionuclide was visualized using autoradiography at predefined time points. The gills did ... Metal Transfer among Organs Following Short- and Long-Term Exposures Using Autoradiography: Cadmium Bioaccumulation by the ...
In vitro autoradiography of receptor-activated G proteins in rat brain by agonist-stimulated guanylyl 5-[gamma-[35S]thio]- ... In vitro autoradiography of receptor-activated G proteins in rat brain by agonist-stimulated guanylyl 5-[gamma-[35S]thio]- ... In vitro autoradiography of receptor-activated G proteins in rat brain by agonist-stimulated guanylyl 5-[gamma-[35S]thio]- ... In vitro autoradiography of receptor-activated G proteins in rat brain by agonist-stimulated guanylyl 5-[gamma-[35S]thio]- ...
Autoradiography. Enzyme Inhibitors / metabolism*. Female. Frontal Lobe / cytology, enzymology*. Humans. Male. Mesencephalon / ... converting enzyme was mapped in the human basal forebrain and midbrain by using quantitative in vitro autoradiography employing ...
Effects of Chronic Treatment with Δ9-Tetrahydrocannabinol on Cannabinoid-Stimulated [35S]GTPγS Autoradiography in Rat Brain. ... Effects of Chronic Treatment with Δ9-Tetrahydrocannabinol on Cannabinoid-Stimulated [35S]GTPγS Autoradiography in Rat Brain ... Effects of Chronic Treatment with Δ9-Tetrahydrocannabinol on Cannabinoid-Stimulated [35S]GTPγS Autoradiography in Rat Brain ... Effects of Chronic Treatment with Δ9-Tetrahydrocannabinol on Cannabinoid-Stimulated [35S]GTPγS Autoradiography in Rat Brain ...
Measurements of glucose utilization by (14C)deoxyglucose autoradiography indicated equivalent metabolic rates for the grafted ... Measurements of glucose utilization by (14C)deoxyglucose autoradiography indicated equivalent metabolic rates for the grafted ... 59 BASIC BIOLOGICAL SCIENCES; BRAIN; PHYSIOLOGY; DOPA; METABOLISM; DOPAMINE; GRAFTS; BIOLOGICAL FUNCTIONS; AUTORADIOGRAPHY; ... 2-deoxy-D-glucose autoradiography. [Rats]}. author = {Schmidt, R H, Ingvar, M, Lindvall, O, Stenevi, U, and Bjoerklund, A}. ...
Autoradiography of Mast Cells in Experimental Skin Tumors of Mice Injected with Radioactive Sulfur (S35). G. Asboe-Hansen ... Stripping-film autoradiography of connective tissue in experimental skin tumors in mice injected intraperitoneally with S35, ... Autoradiography of Mast Cells in Experimental Skin Tumors of Mice Injected with Radioactive Sulfur (S35) ... Autoradiography of Mast Cells in Experimental Skin Tumors of Mice Injected with Radioactive Sulfur (S35) ...
Localization of glycoproteins in insulin secretory granules by ultrastructural autoradiography ... Localization of glycoproteins in insulin secretory granules by ultrastructural autoradiography. Localization of glycoproteins ... in insulin secretory granules by ultrastructural autoradiography. Haddad, A.; Kopriwa, B.; Pelletier, G.. Journal of ...
  • Fig. 2: Definition of regions in autoradiography: via histological stainings (right) it is possible to quantify the receptor density in regions of interest (left. (fz-juelich.de)
  • Various types of receptors, to which the compounds were known to bind to in vitro, were investigated precisely using receptor autoradiography in sections of the same rat brain except for histamine H1 receptors that were measured in the guinea-pig cerebellum. (nih.gov)
  • We now report that this reaction can be used to neuroanatomically localize receptor-activated G proteins in brain sections by in vitro autoradiography of GTP[gamma-35S] binding. (pnas.org)
  • The anatomical distribution of agonist-stimulated GTP[gamma-35S] binding qualitatively paralleled receptor distribution as determined by receptor binding autoradiography. (pnas.org)
  • beta-Adrenergic receptor subtypes were localized and differentiated in rat kidney slices by in vitro autoradiography using the nonselective beta-antagonist [125I]iodocyanopindolol in the presence of the selective agents betaxolol (beta 1) and zinterol (beta 2). (ahajournals.org)
  • In vitro autoradiography provides a useful means to examine different receptor populations in discrete tissue areas. (ahajournals.org)
  • Autoradiography helps to determine the distribution and density of muscarinic receptor (MR) binding sites in the brain. (cas.cz)
  • 35S]GTPgS autoradiography has facilitated the discovery of novel GPCRs, such as the P2Y12 receptor, in the nervous system. (eurekaselect.com)
  • It is evident that the integrity of the basic signalling unit, which for an increasing number of GPCRs resides in specialized membrane microdomains, is better preserved in cryostat sections than in bulk membrane preparations, suggesting that controversial issues, such as constitutive receptor activity in native brain tissue, can be rigorously addressed using [35S]GTPγS autoradiography. (eurekaselect.com)
  • The present study examined the time course of alterations in levels of dopamine transporter (DAT) binding sites that accompany cocaine self-administration using quantitative in vitro receptor autoradiography with [ 3 H]WIN 35,428. (jneurosci.org)
  • Gifford Bioscience Limited is a preclinical contract research organization (CRO) providing specialist services in radioligand binding assays, receptor occupancy studies, autoradiography and SPR. (giffordbioscience.com)
  • Receptor autoradiography was performed on 20 μm sagittal sections of whole postnatal day 0 (P0) male and female prairie voles using the 125 iodinated-ornithine vasotocin ([ 125 I]-OVTA) radioligand. (frontiersin.org)
  • In vitro receptor autoradiography of human nonneoplastic and neoplastic prostate tissue sections with 125 I-[Tyr 4 ]BBN as radioligand indicated high density of GRPR in well-differentiated carcinomas as well as bone metastases, but little or no GRPR was found in hyperplastic prostate and glandular tissue. (snmjournals.org)
  • 64/nat Cu- and 68/nat Ga-NODAGA-LM3 were prepared at room temperature, and 64/nat Cu-CB-TE2A-LM3 and 68/nat Ga-DOTA-LM3 were prepared at 95°C. Binding affinity and antagonistic properties were determined with receptor autoradiography and immunofluorescence microscopy using human embryonic kidney (HEK)-sst2 cells. (snmjournals.org)
  • Angiotensin II (Ang II) receptor subtypes in the rat kidney were investigated by using type 1 (AT1) and type 2 (AT2) Ang II receptor antagonists to discriminate specific 125I-[Sar1,Ile8] Ang II binding sites with in vitro autoradiography. (austin.org.au)
  • Blu-Lite UHC™ is our newest line of high contrast, high sensitivity autoradiography film. (emsdiasum.com)
  • Here, we accessed side effects of recreational drugs such as ketamine, cocaine, and methamphetamine on dopamine neurons in peripheral organs by using positron emission tomography (PET) imaging and quantitative whole-body autoradiography (QWBAR) with [ 18 F]FDOPA. (hindawi.com)
  • Tissue distribution data were obtained by quantitative whole-body autoradiography. (ovid.com)
  • Occupancy of central neurotransmitter receptors by risperidone, clozapine and haloperidol, measured ex vivo by quantitative autoradiography. (nih.gov)
  • 3H]CI-994, a radioactive isotopologue of the benzamide CI-994, a class I histone deacetylase inhibitor (HDACi), was evaluated as an autoradiography probe for ex vivo labeling and localizing of class I HDAC (isoforms 1-3) in the rodent brain. (harvard.edu)
  • The distribution of functionally active monoamine oxidase type A (MAO-A) was investigated by in vivo quantitative autoradiography using [ 14 C]clorgyline in normal, conscious rat brain. (springer.com)
  • Regional 64 Cu distribution in brains was compared by ex vivo autoradiography to that of amyloid-β plaque. (rsc.org)
  • VEGFR, BrdU, lectin staining, and 125 I-VEGF 165 autoradiography on stroke brain tissue slices were performed to validate the in vivo findings. (ahajournals.org)
  • Autoradiography (AR) can also measure quantitative distributions of Mn-52, in ex vivo brain tissue, with spatial resolution similar to MRI. (ubc.ca)
  • Biegon A, Rainbow TC, McEwen BS (1982) Quantitative autoradiography of serotonin receptors in the rat brain. (springer.com)
  • Localization of beta 1- and beta 2-adrenergic receptors in rat kidney by autoradiography. (ahajournals.org)
  • 35S]GTPγS autoradiography is a novel functional approach to detect G protein activity in brain cryostat sections in response to stimulation of G protein-coupled receptors (GPCRs). (eurekaselect.com)
  • 35S]GTPγS autoradiography predominantly detects signalling of receptors that couple to the Gi class of G proteins, and some 20 distinct receptors belonging to this category have been studied to date under various experimental settings. (eurekaselect.com)
  • 35S]GTPγS autoradiography is particularly well-suited for functional studies on brain lysophospholipid receptors, for which conventional radioligand binding assays are not available. (eurekaselect.com)
  • In vitro autoradiography of rat and mouse spleen slices, as spleen expresses a high physiological expression of CB2 receptors, demonstrated that [ 11 C]KP23 exhibits specific binding towards CB2. (mdpi.com)
  • After functional recordings, brain was removed and processed for autoradiography (brain stem sequential slices hybridized with 35 S-oligodeoxynucleotide probes for angiotensinogen [Aogen] and angiotensin II type 1 [AT 1A ] receptors). (ahajournals.org)
  • Leukotriene receptors were examined by autoradiography, and antibody binding to the various enzymes responsible for their formation was assessed by use of immunocytochemistry. (ahajournals.org)
  • Austin Health Research Online: In vitro autoradiography reveals predominantly AT1 angiotensin II receptors in rat kidney. (austin.org.au)
  • In vitro autoradiography reveals predominantly AT1 angiotensin II receptors in rat kidney. (austin.org.au)
  • Using in-vitro autoradiography we analyze a broad range of different neuroreceptors in brain slices (10 -20 µm thick). (fz-juelich.de)
  • Methods: In vitro autoradiography were performed on SV2Alox/Cre+ transgenic mouse brain slices. (ac.be)
  • 5 edition of Positron emission tomography and autoradiography found in the catalog. (perloffphoto.com)
  • Positron Emission Tomography and Autoradiography: Principles and Applications for the Brain and Heart [Phelps, Michael E., Ph.D., Mazziotta, John, Schelbert, Heinrich Positron emission tomography and autoradiography book on *FREE* shipping on qualifying offers. (perloffphoto.com)
  • Positron Emission Tomography and Autoradiography: Principles and Applications for the Brain and Heart. (perloffphoto.com)
  • Schelbert, H R This a text on cerebral and myocardial imaging using positron emission tomography and autoradiography. (perloffphoto.com)
  • Positron Emission Tomography and Autoradiography: Principles and Applications for the Brain and Heart (, Book, Other) Be the first to write a review About this product Pre-owned: lowest price. (perloffphoto.com)
  • This book Positron emission tomography and autoradiography book a must read for physicists, chemists, physicians, and advanced technologists Positron emission tomography and autoradiography book or interested in PET. (perloffphoto.com)
  • It is the definitive reference in the Positron emission tomography and autoradiography book at the time of publication. (perloffphoto.com)
  • Title:Positron Emission Tomography and Autoradiography Imaging of P-selectin Activation Using 68Ga-Fucoidan in Photothrombotic Stroke VOLUME: 15 ISSUE: 1 Author(s):Ina Israel, Felix Fluri, Anders Örbom, Fabian Schadt, Andreas K. (perloffphoto.com)
  • Manganese Imaging with Positron Emission Tomography,Autoradiography, and Magnetic ResonancebyGeoffrey John ToppingA THESIS SUBMITTED IN PARTIAL FULFILMENT OFTHE REQUIREMENTS FOR THE DEGREE OFDOCTOR OF PHILOSOPHYinThe Faculty of Graduate and Postdoctoral Studies(Physics)THE UNIVERSITY OF BRITISH COLUMBIA(Vancouver)February 2014? (ubc.ca)
  • Measurements of glucose utilization by (14C)deoxyglucose autoradiography indicated equivalent metabolic rates for the grafted tissue and the intact substantia nigra. (osti.gov)
  • Absolute quantitative autoradiography of low concentrations of [125I]-labeled proteins in arterial tissue. (harvard.edu)
  • The two hapten molecules comprising 1,4,7-triazacyclononanetriacetic acid chelate moiety (NOTA) were labeled with (67)Ga or (68)Ga. The autoradiography was conducted by incubating the tissue samples with the bispecific antibody TF2, followed by washing and incubation with one of the radiolabeled hapten molecules. (diva-portal.org)
  • CCD (charged coupled device) and CMOS imaging technologies can be applied to thin tissue autoradiography as potential imaging alternatives to using conventional film. (surrey.ac.uk)
  • The Tissue Distribution of the Haematopoetically Active Agents, Cyanocobalamin (57b12) and Inorganic Cobalt (60co), in the Rat Studied by Autoradiography. (semanticscholar.org)
  • I am curious, though, whether or not I can resolve these proteins by SDS/PAGE, transfer to nitrocellulose or PVDF and then detect the labelled proteins by autoradiography. (bio.net)
  • en] Background: SV2A is the most studied isoform of the Synaptic Vesicle 2 proteins, which are involved in the synaptic vesicle trafficking. (ac.be)
  • A high-performance, all-purpose autoradiography film that provides maximum sensitivity. (carestream.com)
  • Also suitable for autoradiography, but with less sensitivity for high-energy isotopes than X-Omat AR and BioMax MS, and than BioMax MR for medium-energy isotopes. (thomassci.com)
  • Where to buy BioMax Autoradiography Film. (carestream.com)
  • Carestream Health, the worldwide leader in developing autoradiography films, sets the standard for research imaging with all our products, from the general purpose BioMax XAR film to the complete BioMax film family. (carestream.com)
  • Site dedicated to scientific imaging systems and autoradiography products. (currentprotocols.com)
  • In summary, we have developed the first known autoradiography tool for imaging class I HDAC enzymes. (harvard.edu)
  • 2006) Digital autoradiography using CCD and CMOS imaging technology In: 15th International Workshop on Room-Temperature Semiconductor X- and Gamma-Ray Detectors/ 2006 IEEE Nuclear Science Symposium, 2006-10-29 - 2006-11-04, San Diego, CA. (surrey.ac.uk)
  • Phosphor imaging is a type of digital autoradiography that maps the distribution of U and Th in rocks and minerals. (columbia.edu)
  • Elekes, K 1978, ' Autoradiography of monoamine uptake in Molluscan ganglia ', Neuroscience Letters , vol. 10, no. suppl. (elsevier.com)
  • Agar Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions suitable for Autoradiography studies and the detection of radioactively labelled samples. (agarscientific.com)
  • In this study, angiotensin converting enzyme was mapped in the human basal forebrain and midbrain by using quantitative in vitro autoradiography employing a derivative of a potent converting enzyme inhibitor, 125I-351A, as radioligand. (biomedsearch.com)
  • Functional activity of substantia nigra grafts reinnervating the striatum: neurotransmitter metabolism and (14C)2-deoxy-D-glucose autoradiography. (osti.gov)
  • This product may be an accessory for KODAK X-OMAT AR (XAR) Autoradiography Film, Carestream Health and may differ from the image shown. (egeneralmedical.com)
  • The surfaces of crushed salt /NaCl/ grains were equilibrated with 36 Cl − to produce a radioactive rim that was imaged by autoradiography. (springer.com)
  • Autoradiography can be used to detect, and measure semiquantitatively, the radioactive materials in almost any object that can be placed in contact with film or photographic emulsion in some form. (thefreedictionary.com)
  • The photographic image of the distribution of radioactive substances in the observed object obtained through autoradiography is called an autoradiogram or radioautograph. (thefreedictionary.com)
  • Autoradiography can help establish the presence of radioactive elements in various ores, the distribution of natural radioactive elements in tissues of plant and animal organisms, and so on. (thefreedictionary.com)
  • Autoradiography is the use of X-ray (or occasionally photographic) film to detect radioactive materials. (nationaldiagnostics.com)
  • Autoradiography of Water-Soluble Substances A. PRECIPITATION M E T H O D In order to prevent the leaching of radioactive water-soluble substances, 32 several methods have been used in autoradiography. (walkingreadinggroup.org)
  • She was a pioneer of the use of radioactive isotopes in biology and medicine, notably the techniques of radiocarbon dating and autoradiography. (wikipedia.org)
  • Page T., Bakay B., Nyhan W.L. (1984) Detection of Hypoxanthine Guanine Phosphoribosyl Transferase Heterozygotes by Thin Layer Chromatography and Autoradiography. (springer.com)
  • Discussions focus on gamma-ray spectrometry, radon detection, autoradiography of uranium and thorium, X-ray diffraction, and application of X-ray analysis. (elsevier.com)
  • Pierre Cau & Jean-Louis Boudier , «Microcomputer programs for quantitative autoradiography and stereology», Acta Stereologica [En ligne], Volume 1 (1982), Number 2 - Stereology 82 - Sheffield - Dec. 1982, 235-238 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=4424. (ac.be)
  • Visualization of brain monoamine oxidase B (MAO-B) in dementia of Alzheimer's type by means of large cryosection autoradiography: a pilot study. (curehunter.com)
  • The anatomical distribution of the radionuclide was visualized using autoradiography at predefined time points. (nih.gov)
  • Rapidly remove haze and artifacts from X-ray film to correct overexposed blots, whether from chemiluminescent Western blot or DNA or RNA autoradiography. (fishersci.com)
  • Advances in autoradiography: A new film brings quality improvements. (currentprotocols.com)
  • A general purpose autoradiography film for use with all commonly used isotopes and chemiluminescent labels. (carestream.com)
  • BluLite™ is a high quality, blue sensitive film that is ideal for a wide range of electrophoretic and autoradiography applications. (thomassci.com)
  • Synonym autoradiography film, xray film Double-emulsion film specifically engineered for biotechnology applications. (thomassci.com)
  • The Autoradiography Ruler Set (ARS) is an orientation and measurement device that exposes metric ruler markings directly onto x-ray film. (thomassci.com)
  • Synonym: autoradiography film, xray film General Description Coated with emulsion on both sides of the clear base. (thomassci.com)
  • KODAK X-OMAT AR (XAR) Autoradiography Film - XAR-5 Film (Alternate Interleaved) : General-purpose film for use with commonly used isotopes and chemiluminescence. (egeneralmedical.com)
  • An increase was also observed in the migration of epithelial cells along the villus, as revealed by [3H]thymidine incorporation and autoradiography in rats fed 900 ppm Sn2+ for 4 weeks. (tudelft.nl)
  • In vitro autoradiography of hippocampal excitatory amino acid binding in aged Fischer 344 rats: relationship to performance on the Morris water maze. (oregonstate.edu)
  • ery versatile for autoradiography with high or low energy isotopes. (thomassci.com)
  • This overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial distribution of radioisotope‐labeled substances within a membrane or specimen material. (currentprotocols.com)
  • SPECT/CT and autoradiography showed a very heterogeneous distribution of 111 In-PD-L1.3.1 within the tumor. (aacrjournals.org)
  • 14C]2-deoxyglucose (2-DG) autoradiography was combined with T2* OC to determine metabolic status of T2*-defined penumbra. (gla.ac.uk)
  • Sixty minutes after [ 14 C]clorgyline administration (1.58 MBq/animal i.v.), the brains were removed and prepared for autoradiography by washing the brain sections with 5% trichloroacetic acid solution to remove the nonbinding free tracer. (springer.com)
  • Quantitative autoradiography, using 3H-deprenyl, was applied to study the regional localization of MAO-B in large cryosections from 3 control brains and 3 brains from patients with Alzheimer's disease (AD). (curehunter.com)
  • 1. Binding sites for calcitonin-gene-related peptide were localized and characterized in porcine coronary arteries using quantitative autoradiography, and the density of binding sites was compared between large epicardial and small intramyocardial coronary arteries. (clinsci.org)
  • Autoradiography is widely used in physics, technology, biology, and medicine-in all cases where isotopic indicators are used. (thefreedictionary.com)
  • Baskerville, T. , McCabe, C. , Weir, C.J. , Macrae, I.M. and Holmes, W. (2012) Noninvasive MRI measurement of CBF: evaluating an arterial spin labelling sequence with 99m Tc- HMPAO CBF autoradiography in a rat stroke model. (gla.ac.uk)
  • The pCASL and 99mTc-HMPAO autoradiography CBF measures, relative to the contralateral hemisphere, were in good agreement across the spectrum of flow values in normal and ischemic tissues. (gla.ac.uk)
  • Here, a pseudo-continuous ASL technique (pCASL) was evaluated against 99mTc-D, L-hexamethylpropyleneamine oxime (99mTc-HMPAO) autoradiography in a rat stroke model. (gla.ac.uk)
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  • Autoradiography with 3H-QNB showed genotype specific loss of binding sites. (cas.cz)
  • High-resolution autoradiography identified specific [ 3 H]-LTC 4 binding sites to smooth muscle cell and to regions of intimal proliferation and plaque. (ahajournals.org)
  • She spent the 1947-48 academic year in the United States learning about the recently discovered techniques of radiocarbon dating and autoradiography, which she introduced to Europe. (wikipedia.org)