The family Lampyidae, which are bioluminescent BEETLES. They contain FIREFLY LUCIFERIN and LUCIFERASES. Oxidation of firefly luciferin results in luminescence.
The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)
Exclusive legal rights or privileges applied to inventions, plants, etc.
Screens which absorb the energy in the x-ray beam that has penetrated the patient and convert this energy into a light pattern which has as nearly as possible the same information as the original x-ray beam. The more light a screen produces for a given input of x-radiation, the less x-ray exposure and thus shorter exposure time are needed to expose the film. In most film-screen systems, the film is sandwiched between two screens in a cassette so that the emulsion on each side is exposed to the light from its contiguous screen.
Gadolinium. An element of the rare earth family of metals. It has the atomic symbol Gd, atomic number 64, and atomic weight 157.25. Its oxide is used in the control rods of some nuclear reactors.
A rapid, low-dose, digital imaging system using a small intraoral sensor instead of radiographic film, an intensifying screen, and a charge-coupled device. It presents the possibility of reduced patient exposure and minimal distortion, although resolution and latitude are inferior to standard dental radiography. A receiver is placed in the mouth, routing signals to a computer which images the signals on a screen or in print. It includes digitizing from x-ray film or any other detector. (From MEDLINE abstracts; personal communication from Dr. Charles Berthold, NIDR)
Colloids formed by the combination of two immiscible liquids such as oil and water. Lipid-in-water emulsions are usually liquid, like milk or lotion. Water-in-lipid emulsions tend to be creams. The formation of emulsions may be aided by amphiphatic molecules that surround one component of the system to form MICELLES.
Facilities that convert NUCLEAR ENERGY into electrical energy.
The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.
A spiro butyrophenone analog similar to HALOPERIDOL and other related compounds. It has been recommended in the treatment of SCHIZOPHRENIA.
A film base coated with an emulsion designed for use with x-rays.
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
Method of analyzing chemicals using automation.
Emulsions of fats or lipids used primarily in parenteral feeding.
A ferroin compound that forms a stable magenta-colored solution with the ferrous ion. The complex has an absorption peak at 562 nm and is used as a reagent and indicator for iron.
Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
A 14-amino acid peptide named for its ability to inhibit pituitary GROWTH HORMONE release, also called somatotropin release-inhibiting factor. It is expressed in the central and peripheral nervous systems, the gut, and other organs. SRIF can also inhibit the release of THYROID-STIMULATING HORMONE; PROLACTIN; INSULIN; and GLUCAGON besides acting as a neurotransmitter and neuromodulator. In a number of species including humans, there is an additional form of somatostatin, SRIF-28 with a 14-amino acid extension at the N-terminal.
Cell surface proteins that bind somatostatin and trigger intracellular changes which influence the behavior of cells. Somatostatin is a hypothalamic hormone, a pancreatic hormone, and a central and peripheral neurotransmitter. Activated somatostatin receptors on pituitary cells inhibit the release of growth hormone; those on endocrine and gastrointestinal cells regulate the absorption and utilization of nutrients; and those on neurons mediate somatostatin's role as a neurotransmitter.
A 29-amino acid pancreatic peptide derived from proglucagon which is also the precursor of intestinal GLUCAGON-LIKE PEPTIDES. Glucagon is secreted by PANCREATIC ALPHA CELLS and plays an important role in regulation of BLOOD GLUCOSE concentration, ketone metabolism, and several other biochemical and physiological processes. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p1511)
Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.
Ovoid body resting on the CRIBRIFORM PLATE of the ethmoid bone where the OLFACTORY NERVE terminates. The olfactory bulb contains several types of nerve cells including the mitral cells, on whose DENDRITES the olfactory nerve synapses, forming the olfactory glomeruli. The accessory olfactory bulb, which receives the projection from the VOMERONASAL ORGAN via the vomeronasal nerve, is also included here.
Tumors or cancer of the gallbladder.
The branch of psychology concerned with similarities or differences in the behavior of different animal species or of different races or peoples.
A class of fibrous proteins or scleroproteins that represents the principal constituent of EPIDERMIS; HAIR; NAILS; horny tissues, and the organic matrix of tooth ENAMEL. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms a coiled-coil alpha helical structure consisting of TYPE I KERATIN and a TYPE II KERATIN, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. alpha-Keratins have been classified into at least 20 subtypes. In addition multiple isoforms of subtypes have been found which may be due to GENE DUPLICATION.
That portion of the nasal mucosa containing the sensory nerve endings for SMELL, located at the dome of each NASAL CAVITY. The yellow-brownish olfactory epithelium consists of OLFACTORY RECEPTOR NEURONS; brush cells; STEM CELLS; and the associated olfactory glands.
Neurons in the OLFACTORY EPITHELIUM with proteins (RECEPTORS, ODORANT) that bind, and thus detect, odorants. These neurons send their DENDRITES to the surface of the epithelium with the odorant receptors residing in the apical non-motile cilia. Their unmyelinated AXONS synapse in the OLFACTORY BULB of the BRAIN.
Increase, over a specific period of time, in the number of individuals living in a country or region.
Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.
Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.
Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety.
An enkephalin analog that selectively binds to the MU OPIOID RECEPTOR. It is used as a model for drug permeability experiments.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
A class of opioid receptors recognized by its pharmacological profile. Mu opioid receptors bind, in decreasing order of affinity, endorphins, dynorphins, met-enkephalin, and leu-enkephalin. They have also been shown to be molecular receptors for morphine.
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
A group of cells that includes FIBROBLASTS, cartilage cells, ADIPOCYTES, smooth muscle cells, and bone cells.
Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.
Inorganic and organic derivatives of sulfuric acid (H2SO4). The salts and esters of sulfuric acid are known as SULFATES and SULFURIC ACID ESTERS respectively.
A potent mast cell degranulator. It is involved in histamine release.
A family of neutral serine proteases with CHYMOTRYPSIN-like activity. Chymases are primarily found in the SECRETORY GRANULES of MAST CELLS and are released during mast cell degranulation.
A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.
A plant genus of the family CRASSULACEAE. Some species in this genus are called stonecrop which is also a common name for RHODIOLA.
A plant genus of the family ASTERACEAE. Members contain a pseudoguaiane sesquiterpene. San-fang-feng is the root of E. grijisii used in DRUGS, CHINESE HERBAL.
Pharmacologic agents delivered into the nostrils in the form of a mist or spray.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
A plant genus in the family CAPRIFOLIACEAE known for elderberries.
A subclass of iridoid compounds that include a glycoside moiety, usually found at the C-1 position.

Ambiguity of the thymidine index. (1/6999)

The observed thymidine indices of seven experimental tumor lines are compared as a function of duration of emulsion exposure. The effects of dose level of tritiated thymidine and background threshold are also evaluated. The results indicate that an arbitrary high background threshold discriminates against "lightly" labeled cells at short periods of exposure but that the chosen threshold becomes less critical with longer exposure. The observed thymidine index increases with increasing duration of emulsion exposure but appears to approach a plateau for all tumor systems. The "thymidine index curves" are significantly different for each tumor. There is an inverse relationship between the dose of tritiated thymidine and the duration of exposure required to recognize the same fraction of cells as labeled in a given tumor. Similar experimental conditions do not necessarily guarantee a valid basis for comparison of observed thymidine indices among tumors.  (+info)

Integrin subunit gene expression is regionally differentiated in adult brain. (2/6999)

Integrins are a diverse family of heterodimeric (alphabeta) adhesion receptors recently shown to be concentrated within synapses and involved in the consolidation of long-term potentiation. Whether neuronal types or anatomical systems in the adult rat brain are coded by integrin type was studied in the present experiments by mapping the relative densities of mRNAs for nine alpha and four beta subunits. Expression patterns were markedly different and in some regions complementary. General results and areas of notable labeling were as follows: alpha1-limited neuronal expression, neocortical layer V, hippocampal CA3; alpha3 and alpha5-diffuse neuronal and glial labeling, Purkinje cells, hippocampal stratum pyramidale, locus coeruleus (alpha3); alpha4- discrete limbic regions, olfactory cortical layer II, hippocampal CA2; alpha6-most prominently neuronal, neocortical subplate, endopiriform, subiculum; alpha7-discrete, all neocortical layers, hippocampal granule cells and CA3, cerebellar granule and Purkinje cells, all efferent cranial nerve nuclei; alpha8-discrete neuronal, deep cortex, hippocampal CA1, basolateral amygdala, striatum; alphaV-all cortical layers, striatum, Purkinje cells; beta4-dentate gyrus granule cells; beta5-broadly distributed, neocortex, medial amygdala, cerebellar granule and Purkinje cells, efferent cranial nerve nuclei; alpha2, beta2, and beta3-mRNAs not detected. These results establish that brain subfields express different balances of integrin subunits and thus different integrin receptors. Such variations will determine which matrix proteins are recognized by neurons and the types of intraneuronal signaling generated by matrix binding. They also could generate important differences in synaptic plasticity across brain systems.  (+info)

Activated macrophages and microglia induce dopaminergic sprouting in the injured striatum and express brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. (3/6999)

Nigrostriatal dopaminergic neurons undergo sprouting around the margins of a striatal wound. The mechanism of this periwound sprouting has been unclear. In this study, we have examined the role played by the macrophage and microglial response that follows striatal injury. Macrophages and activated microglia quickly accumulate after injury and reach their greatest numbers in the first week. Subsequently, the number of both cell types declines rapidly in the first month and thereafter more slowly. Macrophage numbers eventually cease to decline, and a sizable group of these cells remains at the wound site and forms a long-term, highly activated resident population. This population of macrophages expresses increasing amounts of glial cell line-derived neurotrophic factor mRNA with time. Brain-derived neurotrophic factor mRNA is also expressed in and around the wound site. Production of this factor is by both activated microglia and, to a lesser extent, macrophages. The production of these potent dopaminergic neurotrophic factors occurs in a similar spatial distribution to sprouting dopaminergic fibers. Moreover, dopamine transporter-positive dopaminergic neurites can be seen growing toward and embracing hemosiderin-filled wound macrophages. The dopaminergic sprouting that accompanies striatal injury thus appears to result from neurotrophic factor secretion by activated macrophages and microglia at the wound site.  (+info)

Development and cytodifferentiation of the rabbit pars intermedia. II. Neonatal to adult. (4/6999)

Material from pars intermedia obtained from rabbits ranging from the second week post-partum to the adult stage, and including specimens from pregnant animals, was studied. The rate of cell division became greatly reduced early in postnatal) development. The commonest type of cell (the pars intermedia-glandular cell) becomes increasingly PAS-positive during the early stages of development. Although by 35 days differentiation of all the ACT-type cells is complete, the pars intermedia-glandular cells take as long as 53 days to mature. The epithelioid border of the hypophysial cleft persists throughout life, commonly containing dark cells. A ciliary fringe frequently appears in neonates and persists in pregnancy. Possible functions of such cilia are discussed. Throughout development the fine structure of the vasculature was studied. Secretory granules resembling those within the cells were seen in and around the blood vessels, and the mode of endocrine secretion in the pars intermedia tissue is discussed. The pars intermedia-glandular cells of the pregnant rabbits appeared hyperactive. The functional significance of the mammalian pars intermedia is discussed.  (+info)

Onset of nucleolar and extranucleolar transcription and expression of fibrillarin in macaque embryos developing in vitro. (5/6999)

Specific aims were to characterize the onset of nucleolar and extranucleolar transcription and expression of the nucleolar protein fibrillarin during preimplantation development in vitro in macaque embryos using autoradiographic and immunocytochemical techniques. Autoradiography was performed on whole embryos cultured with [3H]uridine for assessment of nucleolar (rRNA) and extranucleolar (mRNA) transcription. Expression of fibrillarin was immunocytochemically assessed in whole embryos using a primary antibody against fibrillarin and a fluorescein isothiocyanate-conjugated secondary antibody. Extranucleolar incorporation of [3H]uridine was first detected in 2-cell embryos cultured 6-10 h with [3H]uridine. Culture with alpha-amanitin prevented incorporation of label in 2-cell embryos, and treatment with ribonuclease reduced the signal to background levels, indicating that [3H]uridine was incorporated into mRNA and not rRNA or DNA. Nucleolar incorporation of [3H]uridine was not evident in pronucleate-stage or 2- to 5-cell embryos, but it was detected in one 6-cell embryo and in all 8-cell to blastocyst-stage embryos. Fibrillarin was first expressed in some 6- to 7-cell embryos, but it was consistently expressed in all 8-cell embryos. Fibrillarin was localized to the perimeter of the nucleolar precursor bodies, forming a ring that completely encapsulated these structures. Fibrillarin was not expressed in 8- to 16-cell embryos cultured with alpha-amanitin, indicating that it is transcribed, rather than recruited, at the 8-cell stage. In conclusion, in in vitro-fertilized macaque embryos developing in vitro, extranucleolar synthesis of mRNA is initiated at the 2-cell stage while the onset of nucleolar transcription occurs at the 6- to 8-cell stage, coincident with expression of fibrillarin.  (+info)

The cerebral metabolic consequences of nitric oxide synthase deficiency: glucose utilization in endothelial and neuronal nitric oxide synthase null mice. (6/6999)

Nitric oxide has multiple physiologic roles in the CNS. Inhibiting nitric oxide synthesis might therefore alter functional activity within the brain. We used [14C]-2-deoxyglucose in vivo autoradiography to measure local CMRglc in "knockout" mice lacking the genes for either the endothelial (eNOS) or neuronal (nNOS) isoforms of nitric oxide synthase, and in the progenitor strains (SV129, C57B1/6). Glucose utilization levels did not significantly differ between nNOS and eNOS knockout mice and C57B1/6 mice in any of the 48 brain regions examined, but were relatively lower in some subcortical regions in SV129 mice.  (+info)

Age-related reductions in [3H]WIN 35,428 binding to the dopamine transporter in nigrostriatal and mesolimbic brain regions of the fischer 344 rat. (7/6999)

In the present study, we used the potent cocaine analog [3H]WIN 35, 428 to map and quantify binding to the dopamine transporter (DAT) within the dorsal striatum, nucleus accumbens, substantia nigra, and ventral tegmental area in young (6-month-old), middle-aged (12-month-old), and aged (18- and 24-month-old) Fischer 344 rats. Quantitative autoradiographic analysis of indirect [3H]WIN 35,428 saturation curves revealed two-site binding for all four brain regions in every age group. The percentage of binding to the high- or low-affinity sites did not differ with age or region and was approximately 50%. However, significant age-related decreases in the overall density (Bmax) of [3H]WIN 35,428-binding sites were observed in the striatum, nucleus accumbens, substantia nigra, and ventral tegmental area. The Bmax within all brain regions declined by more than 15% every 6 months, with the Bmax in the aged (24-month-old) group being approximately half that measured in the young adult (6-month-old) group. Competition experiments indicated that nomifensine also exhibited two-site binding to the DAT in Fischer 344 rats. No consistent age-related differences in binding affinities were noted with either [3H]WIN 35,428 or nomifensine. Taken together, these results support the hypothesis that functional DATs within the nigrostriatal and mesolimbic systems are down-regulated with age, without changing their affinity for ligands.  (+info)

Effect of diabetes and aminoguanidine therapy on renal advanced glycation end-product binding. (8/6999)

BACKGROUND: Advanced glycation end-products (AGEs) have been implicated in the pathogenesis of diabetic nephropathy, and aminoguanidine (AG) has been shown to decrease the accumulation of AGEs in the diabetic kidney. METHODS: This study investigates changes in AGE binding associated with diabetes in the rat kidney using in vitro and in vivo autoradiographic techniques. Male Sprague-Dawley rats were randomized into control and diabetic groups with and without AG treatment and were sacrificed after three weeks. Frozen kidney sections (20 microm) were incubated with [125I]-AGE-RNase or [125I]-AGE-BSA. To localize the AGE binding site, in vivo autoradiography was performed by injection of 15 microCi of [125I]-AGE-BSA into the abdominal aorta of the rat. RESULTS: Low-affinity binding sites specific for AGEs in the renal cortex (IC50 = 0.28 microm) were detected by in vitro autoradiography. There was a significant increase in [125I]-AGE binding in the diabetic kidney, which was prevented by AG treatment. Emulsion autoradiography revealed that binding was localized primarily to proximal tubules in the renal cortex. Renal AGE levels, as assessed by fluorescence or by radioimmunoassay, were increased after three weeks of diabetes. This increase was attenuated by AG therapy. CONCLUSIONS: AGE binding sites are present within the proximal tubules of the kidney and appear to be modulated by endogenous AGE levels. It remains to be determined if these binding sites represent receptors involved in clearance of AGEs or are linked to pathogenic pathways that lead to the development of diabetic nephropathy.  (+info)

After monolayer cultures of rat islets were exposed to [125I]insulin,[125I]glucagon, and [125I]tyrosinyl somatostatin, specific autoradiographic grains associated with each radioactively labeled ligand were found on B, A, and D cells.The density of labeling of the B, A, and D cells with each labeled ligand correlated well with the known actions of the three hormones on each of the islet cells. ...
Many physiological mechanisms as well as numerous neurological diseases involve the complex interaction of various receptors. Quantitative evaluation of the receptor distribution is essential for developing improved procedures in diagnosis and effective treatments of neurological diseases.. Quantitative Receptor Autoradiography. Using in-vitro autoradiography we analyze a broad range of different neuroreceptors in brain slices (10 -20 µm thick). After preincubation in buffer to remove endogenous ligand, sections are incubated with a radioactive substance, that binds specificly to the receptor being studied (radioligand). For the assessment of non-specific binding, selected slices are incubated with the radioligand in presence of an specific antagonist. After washing and a rapid rinse in ice-cold water the sections are dried and exposed against phosphor-imaging plates or conventional film slides in combination with tritium activity standards (see figure 1) for quantitative evaluation. Specific ...
An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance. Alternatively, the autoradiograph is also available as a digital image (digital autoradiography), due to the recent development of scintillation gas detectors or rare earth phosphorimaging systems. The film or emulsion is apposed to the labeled tissue section to obtain the autoradiograph (also called an autoradiogram). The auto- prefix indicates that the radioactive substance is within the sample, as distinguished from the case of historadiography or microradiography, in which the sample is X-rayed using an external source. Some autoradiographs can be examined microscopically for localization of silver grains (such as on the interiors or exteriors of cells or organelles) in which the process is termed micro-autoradiography. For example, micro-autoradiography was used to examine whether atrazine was being ...
TY - JOUR. T1 - Tritiated 2‐deoxy‐D‐glucose. T2 - A high‐resolution marker for autoradiographic localization of brain metabolism. AU - Hammer, Ronald P.. AU - Herkenham, Miles. PY - 1984/1/1. Y1 - 1984/1/1. N2 - The technique for autoradiographic localization of 2‐deoxy‐D‐glucose (2DG) uptake has become a useful method for observing alterations of functional brain activity resulting from experimental manipulation. Autoradiographic resolution is improved using tritiated ([3H]) rather than carbon‐14 ([14C])2DG, due to the lower energy and shorter path of tritium emissions. In addition, lower 2DG uptake by white matter relative to gray matter is exaggerated in the [3H]2DG autoradiographs due to the greater absorption of tritium emissions by lipids. Using [3H]2DG, it is possible to observe differential metabolic labeling in various individual nuclei or portions of nuclei that is unresolvable using [14C]2DG in the awake, normal animal. Heterogeneous patterns of 2DG uptake seen only ...
Various techniques for detection of blood platelets in tissue section were evaluated in an incremental air embolism model of ischemia in order to further investigate the accumulation of platelets in brains subjected to cell-damaging ischemia. The experimental animals were dogs anesthetized with alpha-chloralose. A new autoradiographic technique was devised that allows precise localization of 111Indium-labeled platelets in brain sections. The technique is described in detail along with some effects of the label on platelet function and behavior. The technique can be performed in conjunction with 14C-iodoantipyrine autoradiographic measurement of blood flow without causing mutual interference or other forms of interaction. This permits the simultaneous investigation of local blood flow and deposition of platelets. ...
Double-tracer autoradiographic study of protein synthesis and glucose consumption in rats with focal cerebral ischemia. - T Christensen, T Balchen, T Bruhn, N H Diemer
We have investigated 5-HT1A (serotonin1A) and 5-HT2A (serotonin2A) receptor mRNA abundance and binding site densities in various neocortical and hippocampal regions of schizophrenics and control subjects. Age, agonal state (brain pH), and post mortem interval were included where necessary as covariates in our analyses. In schizophrenics, 5-HT1A binding site densities, determined autoradiographically by [3H]8-hydroxy-2,3-(dipropylamino)-tetralin ([3H]8-OH-DPAT), were significantly increased (+23%) in the dorsolateral prefrontal cortex, with a similar trend in anterior cingulate gyrus. These increases were not accompanied by any change in 5-HT1A receptor mRNA. No differences between the groups in [3H]8-OH-DPAT binding or 5-HT1A receptor mRNA were seen in superior temporal gyrus, striate cortex, or hippocampus. 5-HT2A binding sites, determined by [3H]ketanserin, were decreased in the dorsolateral prefrontal cortex (-27%) and parahippocampal gyrus (-38%) of schizophrenics, with a similar trend in cingulate
Blanks, J C. and Bok, D, An autoradiographic analysis of postnatal cell proliferation in the normal and degenerative mouse retina. (1977). Subject Strain Bibliography 1977. 3447 ...
Mendelsohn, M L., Autoradiographic analysis of cell proliferation in spontaneous breast cancer of c3h mouse. Iii. The growth factor. (1961). Subject Strain Bibliography 1961. 685 ...
Unmodified oligodeoxynucleotides (ODNs) were synthesized and tested for their ability to cross external eukaryotic cell membranes and to enter the cytosol and nucleus in tissue cultures. The ODNs were labeled with high-specific-activity [3H]thymidine (| or = 100 Ci/mmol), or [ alpha-32P]ATP or [ gamma-32P]ATP (300-1000 Ci/mmol; 1 Ci = 37 GBq), and the label was either in the central portion of the molecule or at the 3 or 5 end. The cells employed were for the most part 3T6 murine fibroblasts, grown in monolayers, either semiconfluent or confluent, but some experiments were carried out with chicken embryo fibroblasts or human HeLa cells. Parallel wells in the same experiment were prepared for electron microscopy or for cell fractionation and radioactivity assays. Electron microscopic autoradiography indicated that ODNs cross the external cell membrane, traverse the cytosol, and begin to enter the cell nucleus within a few seconds to 5 min at 37 degrees C in Dulbeccos medium without added serum. After
CCD (charged coupled device) and CMOS imaging technologies can be applied to thin tissue autoradiography as potential imaging alternatives to using conventional film. In this work, we compare two particular devices: a CCD operating in slow scan mode and a CMOS-based active pixel sensor, operating at near video rates. Both imaging sensors have been operated at room temperature using direct irradiation with images produced from calibrated microscales and radiolabelled tissue samples. We also compare these digital image sensor technologies with the use of conventional film. We show comparative results obtained with (14)C calibrated microscales and (35)S radiolabelled tissue sections. We also present the first results of (3)H images produced under direct irradiation of a CCD sensor operating at room temperature. Compared to film, silicon-based imaging technologies exhibit enhanced sensitivity, dynamic range and linearity.. ...
Regional measurements of 131I-9.2.27 distribution in human melanoma tumors were obtained using quantitative autoradiography. Tumors were removed from patients 72-96 h after they had received an i.v. injection of 9.15 mCi (100 mg) of 131I-9.2.27. The autoradiographic images showed that the radioactivity reaching the tumor was heterogeneously distributed. Areas of relative high and low uptake were selected in each tumor. Regions of high activity contained from 51 to 1371 nCi/g, while areas with low uptake had radioactivity ranging from 12 to 487 nCi/g. The reliability of the autoradiographic measurements was demonstrated by the strong positive correlation with direct tissue sample counting (r = 0.994 P , 0.001). Since comparative immunocytochemistry showed a homogeneous and diffuse staining of target antigen on viable tumor cells, variability of monoclonal antibody uptake within individual tumors was not primarily due to heterogeneity of antigen expression in these cases. However, antigen levels ...
The presence and abundance of 5-HT1A and 5-HT2A receptor mRNAs in post mortem human hippocampus was investigated using a novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) technique using cyclophilin mRNA as an internal standard. 5-HT1A and 5-HT2A receptor mRNAs were each co-amplified with varying dilutions of cyclophilin primers, and their abundance expressed as a ratio of cyclophilin mRNA. Using this technique in combination with quantitative autoradiography we have investigated the effect of aging on hippocampal 5-HT1A and 5-HT2A receptor mRNA abundance and binding site densities. There was a significant negative correlation between hippocampal 5-HT1A receptor binding site densities and age and a similar trend for 5-HT1A receptor mRNA abundance. Neither 5-HT2A receptor binding site densities nor mRNA abundance were affected by age. Both 5-HT1A and 5-HT2A receptor binding site densities in individual subjects correlated significantly with abundance of their encoding mRNA. This
Read Quantitative autoradiographic mapping of 5‐HT 3 receptors in the rat CNS using ( 125 I)iodo‐zacopride and ( 3 H)zacopride as radioligands, Synapse on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Distribution of 2-chloro-2 -deoxyadenosine, 2-chloro-2 -arabino-fluoro-2 -deoxyadenosine, Fludarabine and Cytarabine in mice: a whole-body autoradiography study ...
Ling, E.A. (1974). Ultrastructure and radioautographic studies of the thyroid gland. Annals of the Academy of Medicine Singapore 3 (3) : 207-211. [email protected] Repository ...
A technique has been developed that allows repeated autoradiographs to be made of the isotope distribution in the chromosomes of a single cell. A series of 10 separate autoradiographs were made of a Chinese hamster diploid male metaphase cell which had been labeled with tritiated thymidine during the first 15 minutes of its DNA synthesis period in the previous interphase. Each autoradiograph had low grain densities above the chromosomes so that quantitation was feasible. The separate autoradiographs were photographically combined into a single composite in which grain images were converted to lines oriented at right angles to the chromosome axis. The line densities were then measured with a recording microdensitometer to yield graphs reflecting the isotope distribution along each chromosome. The area under each graph was directly proportional to the total number of grains counted above the corresponding chromosome in the 10 separate autoradiographs. The distribution of isotope along the ...
Morphological analysis of transplantable pancreatic acinar carcinoma of rat has revealed a continuum of cells from those which totally lack mature secretory granules to cells with abundant well-formed secretory granules. This tumor is easily dissociable into single cells which incorporate [3H]thymidine and [3H]leucine into DNA and protein at a linear rate for 3 to 4 hr. The number of cells synthesizing DNA, as assessed by light microscope autoradiography, was about 17%. Dissociated neoplastic pancreatic acinar cells are classified into four cell types (types I to IV) on the basis of nuclear morphology, degree of polarization, and the extent of specialization of cytoplasmic organelles. Type IV cells possess all the characteristics of mature pancreatic acinar cells, such as abundant rough endoplasmic reticulum, prominent Golgi apparatus, and numerous secretory granules. Cell types III, II, and I are progressively less differentiated. An analysis of the cytological features of 285 dissociated tumor ...
Agar Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions suitable for Autoradiography studies and the detection of radioactively labelled samples.
The distribution of infused tritiated norepinephrine (NE-3H) in small mesenteric arteries and intestinal arterioles in rats was investigated with electron microscopic radioautography. Silver grains, indicating the presence of the tritium label on the sections, were found lying mainly over axon bundles, but some were present over collagen and smooth muscle cells. Axons with the highest concentrations of silver grains had been sectioned at points where they were naked of Schwann cell sheath, were dilated into varicosities, and contained small granular vesicles. This finding was taken as confirmatory circumstantial evidence that the small granular vesicles were the sites of uptake and storage of NE. The short interval between the start of infusion and the fixation of the tissue appeared to rule out any process other than a direct uptake of NE by the peripheral axons. If axonal sites of uptake of NE-3H correspond to sites of release of NE, then the evidence suggests that such sites of release are ...
The Center for Quantitative Cancer Imaging has as part of its basic science imaging infrastructure a Fuji BAS-5000 Phosphor imager. This device offers extremely high resolution (25 μm) and image quality. It uses Fujifilms unique confocal laser and light-collecting optics. The BAS-5000 is amenable to fine-structure studies of a wide variety of tissue samples. With a dynamic range up to five orders of magnitude and a pixel size as small as 25 μm, the system allows for very high-resolution quantitative autoradiography studies. The system can provide rapid scan times. A 20 x 25 cm imaging plate can be scanned at 50 μm in as little as five minutes. The system includes software for region of interest (ROI) analysis and quantitative assessment of tracer accumulation. The system can image all PET isotopes as well as C-14 and tritium. The system also has the capabilities to perform various optical-based assays, including 2D electrophoresis and thin layer chromatography.. ...
Conceptual computer illustration of the DNA double helix together with a graphic representation of an autoradiograph display. The pattern of the DNA autoradiograph bands is unique to each individual, but some bands are shared by related people, such as a parent & child. DNA fingerprints can be used to prove conclusively whether people are related. - Stock Image C010/5265
The distribution of cells expressing mu-receptor mRNA and mu-receptor binding sites were compared in brain and spinal cord tissue sections using a combination of in situ hybridization and receptor autoradiographic techniques. mu-Receptor mRNA was visualized with a 35S-labeled cRNA probe directed to transmembrane III-VI of the rat mu-receptor, while mu-receptor binding sites were labeled with the mu-selective ligand [3H]DAMGO ...
TY - JOUR. T1 - Prediction of histopathological outcome using averaged multimodal information in rat. AU - Zhao, Weizhao. AU - Ginsberg, Myron D.. AU - Belayev, Ludmila. AU - Truettner, Jessie. AU - Schmidt-Kastner, Rainald. PY - 1998/12/1. Y1 - 1998/12/1. N2 - Commonly used autoradiographic image analysis for stroke research has been restricted to the assessment of local cerebral blood flow (LCBF), glucose utilization (LCMRglc) or messenger RNA (mRNA) (by in situ hybridization) in individual brains, with repeated measurements from the same animal. Histopathological analysis of perfusion-fixed paraffin-embedded brain material has long been regarded as the `gold standard for the quantitative assessment of tissue injury in experimental models of cerebral ischemia. It is desirable to reveal the interrelationship between signals measured at the time of ischemia and the final distribution of cell damage. In this study, averaged images for each modality were derived from individual rats and ...
The IVISR provides optical, CT, ultrasound, high-resolution planar gamma imaging, and PET of mice and rats, as well as high-resolution digital autoradiography and fluorescent imaging of thin whole body rodent sections.
The autoradiographic analysis of neurotransmitter receptor distribution is a powerful technique that provides extensive information on the localization of neurotransmitter systems. Computer methodologies are described for the analysis of autoradiographic material which include quench correction, 3-dimensional display, and quantification based on anatomical boundaries determined from the tissue sections. These methodologies are applied to the problem of the distribution of glycine receptors measured by 3H-strychnine binding in the mouse CNS. The most distinctive feature of this distribution is its marked caudorostral gradient. The highest densities of binding sites within this gradient were seen in somatic motor and sensory areas; high densities of binding were seen in branchial efferent and special sensory areas. Moderate levels were seen in nuclei related to visceral function. Densities within the reticular formation paralleled the overall gradient with high to moderate levels of binding. The ...
Figure 1 Antiphospho-Pak1 antibody specifically recognizes activated Pak1. (A) Activation loop sequence of the phosphorylated peptide used for production of polyclonal antiphospho-Pak1 antisera. (B) Immunoblots (lanes 1-4) and radiolabeled kinase reaction products (lanes 5 and 6) of baculovirus-produced Pak1. Purified KD or CA Pak1 was incubated in protein kinase buffer in the presence of ATP, then immunoblotted using anti-Pak1 (lanes 1 and 2) and antiphospho-Pak antisera (lanes 3 and 4) autoradiographed to detect antibody-bound proteins. Although both forms of the protein were detected with anti-Pak1, only activated Pak1 was detected using the phospho-specific antibody, despite the large amount of recombinant protein used in this assay. Lanes 5 and 6 show autoradiographed PAGE gel of reaction products from a kinase assay performed in the presence of [γ-32P]ATP. Molecular mass in kilodaltons is shown to the right of the blot. (C) Comparison between immunoblot and radiolabeled kinase reaction ...
PCR products were separated by electrophoresis (Protean-2, Biorad, Issy-les-Moulineaux, France) on 8% polyacrylamide gels, which were then vacuum dried and processed for autoradiography on Biomax films (Kodak, Sevran, France). Exposure times were adjusted to avoid film saturation. For each primer pair, we first determined the optimal hybridization temperature and minimum number of PCR cycles necessary to detect radiolabeled reaction products at a unique, expected molecular size, so that each PCR analysis would be performed in the exponential phase of amplification. Forward and reverse primers were designed in separate exons to avoid any bias caused by residual genomic contamination. PCR-amplified products were checked with restriction enzymes. For all primer pairs, no amplification was observed when PCR was performed on either RNase-free water or non-reverse-transcribed RNA samples. At least three independent PCRs were performed on the same samples for each primer pair.. Optical densities (ODs) ...
The first concerns cellular mechanisms of learning and memory. There is considerable evidence that a crucial step in memory formation is the synthesis of new proteins in the hippocampus. We are using autoradiographic techniques to focus on proteins that are made in dendrites of hippocampal neurons, near synapses. Very few proteins are made there, but we have found out that electrical stimulation of the axons that make contact with these dendrites greatly stimulates their synthesis of proteins. This only happens when the stimulus is combined with another neurotransmitter, acetylcholine. There is reason to believe this phenomenon is important in memory formation and we are studying its mechanisms and significance. The second area concerns the regulation of synaptic transmission by glycolysis. We have found that synaptic transmission in the hippocampus will only occur if glycolysis is active, even if adequate energy is supplied in other ways. This is very unexpected. There appears to be a ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Ive had a decades-long fascination with herbs and natural healing and found over the years that many such treatments can be quite successful. Im...
The Ghana Socioeconomic Panel Survey 2009-2010 collected baseline information for a panel of 5,009 households (18,889 household members) across all regions of Ghana. Two questionnaires collected information about household demographics, housing characteristics, household and agricultural assets, agricultural production, household enterprises, health and fertility, and household consumption and expenditure. Cognitive tests were conducted for children in the household, and psychological tests were conducted for the household head, spouse, and a randomly chosen household member.. A community questionnaire was also used to collect information about community institutions, political organizations, development programs, and infrastructure. Geographic (GPS) information was also recorded for all households and community facilities.. ...
Generate more awareness for your business with branded car magnets. These versatile magnets offer a less permanent option over decals or wraps.
The intracellular transport of venom proteins has been studied in active and resting venom glands of the snake Vipera palaestinae by electron microscope radioautography after an intra-arterial injection of [3H]leucine. In the active gland, most of the label is initially (10 min) found over the RER. By 30 min, the relative grain density of the Golgi complex reaches its maximum, with concomitant increase in the labeling of the condensing vacuoles. Later on, a steep increase in radioactivity of the secretory granules is observed. At 3 h, these granules, which comprise about 2% of the cell volume, contain 22% of the total grains. At the following hour, their labeling declines and at the same time the radioactivity of the secreted venom is increased. It is concluded that, in the active cell, venom proteins are transported via the Golgi apparatus into membrane-bounded granules which are the immediate source of the secreted venom. An alternative pathway, which involves the RER cisternae as a storage ...
Grain counting by eye is a tedious and time-consuming technique but one with great potential in cell kinetics and for the study of DNA excision repair activity (unscheduled DNA synthesis or UDS). We have been investigating the levels of UDS in human skin sections exposed in situ to ultraviolet radiation using a short-term incubation in tritiated thymidine and autoradiography and the decline in UDS levels with time (repair kinetics). We have adapted an automated image analysis system automatically to assess the number of grains over epidermal cell nuclei in autoradiographs of sections of epidermis. An excellent correlation was observed between visual counting and machine measurement of the area (in pixels) occupied by silver grains. The levels of UDS declined with time as lesions are progressively repaired. The half time (+/- standard deviation) for the reduction in UDS is 7.25 +/- 0.18 h. The grain counts can be significantly increased by increasing the autoradiographic exposure, by increasing ...
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The publisher regrets that a mistake was published in this article. On page 747, the word antagonist was incorrectly published instead of agonist. The whole sentence should correctly read: Importantly, also in response to an adenosine A1 agonist, old rats had less sleep compared with young animals. Our sincere apologies are offered to the authors, editor, and readers.. The original article of this erratum was published in J Neurosci Res 2004; 78: 742-748 ...
Charles River designs and conducts GLP-compliant quantitative whole-body autoradiography (QWBA) programs integrated with our preclinical drug metabolism services.
An assemblage useful for autoradiography comprising, in order, a sample layer containing a radioactively labeled biological sample in contact with a phosphor layer, a film layer and a reflector layer. The phosphor layer is preferably a gadolinium oxysulfide containing layer and the film is preferably a photographic film containing a tabular grain emulsion.
TY - JOUR. T1 - Autoradiography of monoamine uptake in Molluscan ganglia. AU - Elekes, K.. PY - 1978/1/1. Y1 - 1978/1/1. UR - http://www.scopus.com/inward/record.url?scp=0018084061&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0018084061&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0018084061. VL - 10. SP - S268. JO - Neuroscience Letters. JF - Neuroscience Letters. SN - 0304-3940. IS - suppl. 1. ER - ...
Read independent reviews on EN3HANCE™ Spray Surface Autoradiography Enhancer, 2oz Spray Can from PerkinElmer, Inc. on SelectScience
DNA. Computer artwork of a DNA (deoxyribonucleic acid) double helix seen against autoradiograms of genetic sequences (upper right). The spiralling strands of the DNA helix (blue) are composed of complex chemical groups called nucleotides, which consist of a sugar phosphate and a base group. Complementary pairing between the base groups of nucleotides on opposite strands holds the helix together. The sequence of the 4 base groups (adenine, cytosine, guanine and thymine; coloured rods) along the DNA helix is unique for every individual and is known as the genetic code. The autoradiograms depict DNA base sequences, each band (dark blue) representing an individual base. - Stock Image G110/0642
The effect of aerosol upon cloud properties is studied over the Baltic Sea region, which presents a distinct contrast of aerosol loading between the clean Fennoscandia and the polluted area of central-eastern Europe. Statistically significant positive values are found over the Baltic Sea and Fennoscandia, while negative values are found over central-eastern Europe, contradicting the theory of aerosol indirect effect on clouds ...
A variety of methods have been used in published studies for image segmentation (manual outlining, thresholding, magic wand, use of templates, etc.) to determine what is part of the structure or region of interest and what is not. Similarly, once grey levels are measured, a variety of methods have been employed to convert them into optical densities or units of radioactivity. Based on the above results, the line method was the best way to select the structure or region of interest as it was not subject to floor-effects, had a low coefficient of variation, and low within-sample variability. This method is similar to, and a modification of the outline method, which requires that the actual boundaries of the structure be determined, whereas the line method sampled only from the interior of the structure. Since the DG is a long narrow structure, this was best done with a line down the centre. Larger structures can follow the same principle by outlining the structure but staying well inside the ...
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There are 13 fifteen-letter words containing A, D, G, U and Y: AUTORADIOGRAPHY DAGUERREOTYPERS DAGUERREOTYPIES ... PSEUDOPREGNANCY RADIOAUTOGRAPHY UNDERSTANDINGLY. Every word on this site can be used while playing scrabble. Create other lists, beginning with or ending with letters of your choice.
A Duke University study that found over 40 percent of our actions aren’t actually decisions, but habits. Heres how to build good ones. 
TY - JOUR. T1 - Characteristics of muscarinic cholinergic, γ-aminobutyric acidA and phencyclidine receptors in spontaneously epileptic rats; in vitro quantitative autoradiographic analysis. AU - Murase, Kenshi. AU - Nabeshima, Toshitaka. AU - Kameyama, Tsutomu. AU - Sasa, Masashi. AU - Takaori, Shuji. AU - Ujihara, Hisamitsu. AU - Ishihara, Kumatoshi. AU - Yamada, Junzo. AU - Serikawa, Tadao. PY - 1991/9/30. Y1 - 1991/9/30. N2 - Characteristics of muscarinic cholinergic (mACh), γ-aminobutyric acidA (GABAA) and phencyclidine (PCP) receptors in the spontaneously epileptic rats (SER), which exhibit both absence-like seizures and tonic convulsion, were examined using in vitro quantitative autoradiography. Computer analysis using autoradiographic technique revealed that the amount of the specific binding of [3H]quinuclidinyl benzilate (QNB) to mACh receptors in the striatum of SER was more than that of zitter rats, not exhibiting both seizures and convulsion. However, the specific bindings of ...
Clinically effective antidepressants are thought to exert their therapeutic effects by facilitating central monoamine neurotransmission. However, recent data showing that neurokinin-1 receptor (NK1R) antagonists have antidepressant properties in both animal and clinical studies raise the possibility that classical antidepressants may also influence NK1R expression in the brain. To test this hypothesis, rats were treated with desipramine, paroxetine, venlafaxine, tranylcypromine or vehicle for 14-42 days. NK1R binding sites and mRNA were determined in a wide variety of brain areas using in situ hybridization and quantitative receptor autoradiography. In all areas examined, the abundance of NK1R binding sites was unchanged after 14 days of treatment. None of the treatments altered the number of NK1R binding sites following 42 days treatment with the exception that an increase was found in the locus coeruleus with tranylcypromine. Taken together, we report that repeated treatment with antidepressants of
1. Binding sites for calcitonin-gene-related peptide were localized and characterized in porcine coronary arteries using quantitative autoradiography, and the density of binding sites was compared between large epicardial and small intramyocardial coronary arteries.. 2. A single class of binding sites for calcitonin-gene-related peptide with a dissociation constant of 2.1 ± 0.2 nmol/l was detected in both the large and small coronary arteries. The density of specific binding sites was higher (maximum binding site density 231 ± 14 fmol/mg of protein) in the small coronary arteries than in the large epicardial coronary arteries (maximum binding site density 108 ± 5 fmol/mg of protein). β-Human calcitonin-gene-related peptide showed higher affinity than α-human calcitonin-gene-related peptide for the binding sites. Most of the specific binding sites for both peptides in the large coronary artery were localized in the intima and media.. 3. In coronary artery from patients with coronary heart ...
THE ANATOMICAL RECORD 222:191-200 (1988) Radioautographic Demonstration of Receptors for Epidermal Growth Factor in Various Cells of the Oral Cavity MOON-IL CHO, YU LIN LEE, AND PHILIAS R. GARANT Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook, New York 11794-8700 ABSTRACT Mouse iodinated epidermal growth factor (EGF)was localizedby light and electron microscopic radioautography in basal cells of oral epithelium, papillary cells of the enamel organ, periodontal ligament fibroblasts, preodontoblast precursor cells, and preosteoblasts of the alveolar bone of 13-day-old Sprague-Dawleyrats. The specificity of binding in these cells was suggested by an observed reduction of about 90% in the labeling when excess unlabeled EGF was injected along with the 125I-EGF. In contrast, fully differentiated cells, such as ameloblasts. odontoblasts, and osteoblasts, were only poorly labeled. Quantitative analysis of the light microscopic ...
The distribution of radioactivity was studied autoradiographically in the uterus of the ovariectomized rat from 1 to 7 h after the s.c. injection of [3H]progesterone. Luminal and glandular epithelia were less radioactive than stroma or muscle. Grain densities over nuclei were the same as those over cytoplasm in the epithelial tissues and the muscle. Pretreatment with non-radioactive progesterone did not alter the pattern of distribution of radioactivity though in one experiment grain densities were significantly decreased in the pretreated animal; this decrease involved nucleus and cytoplasm in epithelial cells and in the muscle.. The interpretation of grain densities after the administration of [3H]progesterone is complicated by the presence of labelled metabolites. Further experiments were therefore carried out with [3H]megestrol acetate, a progestin which is not significantly metabolized in the uterus or plasma during the first 3 h after injection s.c. At 30 min, 1 and 2 h after [3H]megestrol ...
Agonists stimulate guanylyl 5-[gamma-[35S]thio]-triphosphate (GTP[gamma-35S]) binding to receptor-coupled guanine nucleotide binding protein (G proteins) in cell membranes as revealed in the presence of excess GDP. We now report that this reaction can be used to neuroanatomically localize receptor-activated G proteins in brain sections by in vitro autoradiography of GTP[gamma-35S] binding. Using the mu opioid-selective peptide [D-Ala2,N-MePhe4,Gly5-ol]enkephalin (DAMGO) as an agonist in rat brain sections and isolated thalamic membranes, agonist stimulation of GTP[gamma-35S] binding required the presence of excess GDP (1-2 mM GDP in sections vs. 10-30 microM GDP in membranes) to decrease basal G-protein activity and reveal agonist-stimulated GTP[gamma-35S] binding. Similar concentrations of DAMGO were required to stimulate GTP[gamma-35S] binding in sections and membranes. To demonstrate the general applicability of the technique, agonist-stimulated GTP[gamma-35S] binding in tissue sections was ...
Glycoproteins were metabolically labeled with 3H-fucose in cultured RPE cells from RCS rdy-p+ and Long Evans rats. 3H-labeled glycoproteins associated with a plasma membrane-enriched subcellular fraction were separated by two-dimensional gel electrophoresis. Relative incorporation of 3H-fucose into high molecular weight cell surface glycoproteins (Mr of 128,000-183,000) was measured by quantitative autoradiography and densitometry. The results of these experiments show that 3H-fucose incorporation into four glycoproteins (Mr of 183,000, 175,000, 164,000 and 156,000) was reduced by 30-50% in the dystrophic RPE as compared to the normal cells. This reduction was not due to an absence of the protein core of glycoproteins on the dystrophic RPE cell surface; when RPE cells were labeled with 3H-leucine prior to analysis, no reduction in label was found in the dystrophic RPE as compared to normal. Therefore, the results of this study suggest that the RCS rat RPE processes the oligosaccharide portion of ...
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Abstract Migration and elimination of 75Se-labeled Schistosoma mansoni were studied in previously infected mice by means of autoradiography and worm recovery. As in control mice, there appeared to be little if any worm elimination in the skin of previously infected mice. In previously infected mice, there appeared to be some worm elimination in the lungs and in migration sites between the lungs and liver, though much less than would have been expected from previous studies. In two of three experiments most of the challenge worm elimination in previously infected mice, above the normal attrition level occurring in the controls, appeared to take place in the liver. In the third experiment, it appeared that all of the challenge worm elimination occurred after migration to the lungs, and at least one-third of it in the liver. It was also observed that the lung chop procedure recovered viable schistosomula much less efficiently from previously infected mice than from controls, indicating that the reduction
Figure 3. Ocular clusterin localization by immunohistochemistry and in situ analysis. Shown are sections of cornea (panels A-C, serial sections), skin/eyelid (panels D, E, serial sections), ciliary body (unfilled arrow and * in panel F), and lens (panels G, H, serial sections). Immunodetection with G7 are shown in panels B, E, F, and H; panels A, D, and G are the negative controls in which the G7 antibody was omitted. In situ hybridization of an anti-sense clusterin mRNA probe on cornea is shown in panel C. Panel I: graph illustrating the distribution of silver grain over different regions of the cornea shown in panel C, for each general region 10 independent counts within a fixed area were taken. Solid arrows indicate the interface between epithelial and stromal cells (cs) in the cornea, arrow heads indicate the area of epithelial cells in the lens.. ...
Apixaban, a potent and highly selective factor Xa inhibitor, is currently under development for treatment of arterial and venous thrombotic diseases. The distribution, metabolism, and elimination of [14C]apixaban were investigated in male, female, pregnant, and lactating rats after single oral doses. Tissue distribution of radioactivity in rats was measured using quantitative whole-body autoradiography. After a single oral administration, radioactivity distributed quickly in rats with Cmax at 1 h for most tissues. The elimination t1/2 of radioactivity in blood was 1.7 to 4.2 h. The blood area under the plasma concentration-time curve of radioactivity was similar between male and female rats and was slightly higher in pregnant rats and lower in lactating rats. The radioactivity concentration in tissues involved in elimination was greater than that in blood with the highest concentration in the gastrointestinal tract, liver, and urinary bladder/contents and lowest level in brains. In pregnant ...
Abstract The influence of the melt flow on the solidification structure is bilateral. The flow plays an important role in the solidification pattern, viathe heat transfer, grain distribution, and...
Labeled antagonists have advantages over agonists for the characterization of G protein-coupled receptors if, for example, the total number of binding sites is of interest, because the binding of antagonists is independent of the fraction of receptors coupled to the GTP-binding proteins (DeLean et al., 1980). For example, in thebeta adrenergic receptor system, high-affinity antagonists were used in receptor characterization (Brown et al., 1976).. All previously reported CRF antagonists were of too low an affinity to be used as radioligands for characterizing CRF-R1 receptors. With astressin (Gulyas et al., 1995), we now have an antagonist of sufficient affinity to make it suitable as a radioligand for the detection and characterization of both cloned and endogenous CRF receptors. Using autoradiographic techniques and radioreceptor assays, brain receptors have also been detected with oCRF* (De Souza et al., 1984) and 125I-labeled [Tyr0]oCRF (Grigoriadis and De Souza, 1988;Webster et al., ...
Since the classical paper of Leblond & Stevens (1948), much information on cell renewal in the intestinal epithelium has become available. The introduction of tritiated thymidine autoradiography has diverted attention from conspicuous lacunae which still exist in our knowledge of intestinal structure and growth. This paper attempts to fill two of these elementary gaps. The first is well defined by Fry, Lesher, Kisieleski & Sacher (1963): we still remain ignorant even of the complete three-dimensional structure of the mucosa in any one region. It seems incredible that such a problem was not attacked by the great German microanatomists of the last century, but no accurate account of the three-dimensional structure of the intestinal mucosa is known to me; the accounts in present-day textbooks of histology in the English language are superficial and even inaccurate (see, for example, Bloom & Fawcett, 1962; Ham, 1965).. ...
A sample study was performed where a designed set of primers specific to the Y chromosome from the GRCh 38 Human Genome was queried in both ThermoBLAST CE and BLAST to compare the number of extensible hits and the number of false amplicons that were detected. The results showed that ThermoBLAST CE detected 765 unique extensible hits in the Y chromosome while BLAST detected only 15. Furthermore, ThermoBLAST CE detected a total of 946 extensible hits for the forward primer design, where BLAST detected only 52 extensible hits. For the reverse primer ThermoBLAST found over 98% more hits than BLAST, and likewise for the forward primer ThermoBLAST CE found over 94% more hits than BLAST. Questions about this study can be directed to DNA Software, Inc.. ThermoBLAST CETM = Cloud Integration + Custom Genome Playlists+Genome Viewer+The Computational Capacity To Use It. ...
If checking nutritional labels is like reading Chinese, you are in good company. Nutrition is complicated and a lot of the information is contradictory. The tips youre about to read will clear up your ideas of nutrition. Try to stock up on whole grains over white flour products. Fiber and protein-rich whole grains are always... » read more. ...
Proximas Horsing Around Page is a collection of links to various equestrian Pages that I have found over the Internet, a (growing) list of Toxic Plants, some humor, and pictures of my horses, both regular-sized and minis!
Create healthcare diagrams like this example called Coronal Brain Sections in minutes with SmartDraw. SmartDraw includes 1000s of professional healthcare and anatomy chart templates that you can modify and make your own.
Durie, B.; Salmon, S. (1975-12-12). "High speed scintillation autoradiography". Science. 190 (4219): 1093-1095. doi:10.1126/ ...
Autoradiography was then used by the Finsen Institute to investigate the effects of the radiocontrast agent thorotrast. Levi ... She developed the new technique of autoradiography while working for the United States Atomic Energy Commission at the ... Levi, Hilde; Boyd, George A. (20 January 1950). "Carbon 14 Beta Track Autoradiography". Science. 111 (2873): 58-59. Bibcode: ... notably the techniques of radiocarbon dating and autoradiography. In later life she became a scientific historian, and ...
... in which the process is termed micro-autoradiography. For example, micro-autoradiography was used to examine whether atrazine ... For in vitro autoradiography, radioligand was directly applying on frozen tissue sections without administration to the subject ... The rate of DNA replication in a mouse cell growing in vitro was measured by autoradiography as 33 nucleotides per second. The ... Applications for autoradiography are broad, ranging from biomedical to environmental sciences to industry. The use of ...
"Photographic image intensification by autoradiography". Applied Optics. 15 (11): 2860-2865. Bibcode:1976ApOpt..15.2860A. doi: ...
They used tritiated thymidine autoradiography to label the cells. Tritiated thymidine is incorporated into the DNA of dividing ... since no phenotypic markers were available that could be used in conjunction with thymidine autoradiography. The absence of ...
Densita allows researchers to analyze autoradiography and optical density. Vesselucida: software for 3D microvascular tracing ...
Huberman, J. A.; Riggs, A. D. (March 1, 1966). "Autoradiography of chromosomal DNA fibers from Chinese hamster cells". ...
To detect any protein-DNA interactions, autoradiography is commonly used. "Southwestern blot mapping" is a time-efficient way ...
CI-994 autoradiography". Epigenetics. 8 (7): 756-764. doi:10.4161/epi.25202. ISSN 1559-2308. PMC 3781195. PMID 23803584. ...
Thus the surviving probe-mRNA complement is simply detected by autoradiography. Nuclease protection assays are used to map ...
He was the first to map the distribution of receptors in the lung using radioligand autoradiography. His group investigated the ... Localization of ß-adrenoceptors in mammalian lung by light microscopic autoradiography. Nature 1982; 299:444-447. PMID 6289123 ...
This is followed by autoradiography to detect the position of the label. The plaque hybridization procedure has some advantages ...
As professor of neurobiology at Cornell University, she developed quantitative electron microscopic autoradiography as a means ... She developed quantitative electron microscopic autoradiography, and demonstrated it as a sensitive means to study the ... Salpeter, Miriam M. (1964). "Autoradiography with the electron microscope: a procedure for improving resolution, sensitivity, ... "Quantitation of junctional and extrajunctional acetylcholine receptors by electron microscope autoradiography after (125)I-α- ...
Bacteriophage T4 replicating DNA was labeled with tritiated thymidine and examined by autoradiography. The observed DNA ...
These could be fractionated by electrophoresis on a polyacrylamide gel and visualised using autoradiography. The procedure ...
... parallel observations from diffusion spectrum imaging and autoradiography". Brain. 130 (Pt 3): 630-53. doi:10.1093/brain/awl359 ...
Blue Devil Film is used for blotting, sequencing, chemiluminescent autoradiography (ecl) and gel shift analysis. It is designed ... Genesee Scientific has also been responsible for developing Blue Devil autoradiography film which produces exceptional clarity ... Blue Devil provides film is used for autoradiography, Western blotting, sequencing, chemiluminescence and gel shift analysis. ...
Quantitative Whole Body Autoradiography (QWBA): Larger than micro-autoradiography, whole animals, typically rodents, can be ... Autoradiography: A tissue section affixed to a microscope slide or a membrane such as a Northern blot or a hybridized slot blot ... Micro-autoradiography: A tissue section, typically cryosectioned, is placed against a phosphor screen as above. ...
... is allowed to penetrate small cracks, and then its presence is detected by autoradiography. The method is called " ...
Experimental thyrotoxic myopathy - autoradiography of protein-synthesis in skeletal-muscle and motor-neurons of spinal-cord. ...
May 2012). "Nicotinic acetylcholine receptors in rat forebrain that bind ¹⁸F-nifene: relating PET imaging, autoradiography, and ...
Alternatively, radioactive elements can be used as labels, and the immunoreaction can be visualized by autoradiography. Tissue ...
... receptor autoradiography and gene expression in rat brain". Neuroscience Letters. 204 (1-2): 121-4. doi:10.1016/0304-3940(96) ...
Their autoradiography observations on regenerating limbs also proved that mononuclear blood cells are the source of osteoclasts ...
The new techniques, combined with autoradiography, allowed her to study these processes in living cells and tissue. In 1956, ...
Her experiments using autoradiography revealed cocaine's effect on D1 and D2 dopamine receptors in the rat striatum. Rats ...
A posthumously published autoradiography of an amputated leg with strontium-89 is the first human bone scintigraphy. It was the ...
... or by autoradiography, i.e., fogging X-Ray film. Their age and origin can be determined by their isotopic signature. Due to ...
The products can then be analyzed by gel electrophoresis and the 35S-labeled peptide is visualized using autoradiography. There ...
... nisoxetine autoradiography". Brain Research. 582 (2): 208-14. doi:10.1016/0006-8993(92)90134-U. PMID 1327403. S2CID 25244627. ... can be used to map anatomical sites associated with norepinephrine uptake through the technique of quantitative autoradiography ...
... Labsupply labsupply at aol.com Tue May 5 23:09:59 EST 1998 *Previous message: Practical Ion Channel ... labscientific, inc sells inexpensive autoradiography film suitable for ecl, western blots, sequencing and gel shift analysis, ...
Recommended for direct autoradiography of weak and medium energy β-radioisotopes, such as 35S,33P, and 14C ... whether from chemiluminescent Western blot or DNA or RNA autoradiography. ...
... Samuel C. Blackman scb1 at midway.uchicago.edu Fri May 16 15:22:51 EST 1997 *Previous ... transfer to nitrocellulose or PVDF and then detect the labelled proteins by autoradiography. Ive heard that it is possible if ...
An assemblage useful for autoradiography comprising, in order, a sample layer containing a radioactively labeled biological ... 1995 , entitled AUTORADIOGRAPHY ASSEMBLAGE USING TRANSPARENT SCREEN.. FIELD OF THE INVENTION. The present invention is directed ... This field is sometimes referred to as autoradiography.. BACKGROUND OF THE INVENTION. An autoradiograph is a recording of the ... Electronic imaging system for autoradiography. US6521329 *. 18 Jun 2001. 18 Feb 2003. Eastman Kodak Company. Radiographic ...
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Quantitative autoradiography of serotonin receptors in the rat brain. Brain Res. 242: 197-204.PubMedCrossRefGoogle Scholar ... Serotonin receptor localization in rat brain by light microscopic autoradiography. Eur. J. Pharmacol. 62: 237-239.PubMed ... Receptor autoradiography with tritium-sensitive film: potential for computerized densitometry. Neuroscience Lett. 25: 101-105. ... Estradiol-concentrating neurons: Topography in the hypothalamus by dry-mount autoradiography. Science 162: 1001-1003.PubMed ...
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Scientists studying a DNA sequencing autoradiogram of novel recombinant proteins cloned for the production of VaxSyn, an experimental AIDS vaccine. VaxSyn is based on a genetically- engineered, recombinant form of the HIV protein gp160, a precursor to one of HIVs surface proteins. VaxSyn contains no attenuated infectious parts of HIV & therefore carries no risk of transmitting AIDS. The vaccine is being developed by MicroGeneSys and is undergoing trials as a treatment of HIV-positive patients & as a potential vaccine against HIV infection. - Stock Image G210/0383
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  • The development of oxytocin (OT) receptors in the rat brain and spinal cord was studied by in vitro light microscopic autoradiography and by electrophysiology. (pasteur.fr)
  • Fig. 2: Definition of regions in autoradiography: via histological stainings (right) it is possible to quantify the receptor density in regions of interest (left. (fz-juelich.de)
  • Various types of receptors, to which the compounds were known to bind to in vitro, were investigated precisely using receptor autoradiography in sections of the same rat brain except for histamine H1 receptors that were measured in the guinea-pig cerebellum. (nih.gov)
  • We have studied which cell type is induced to divide during receptor cell regeneration stimulated by olfactory bulbectomy using a combination of immunocytochemistry and autoradiography. (jneurosci.org)
  • We now report that this reaction can be used to neuroanatomically localize receptor-activated G proteins in brain sections by in vitro autoradiography of GTP[gamma-35S] binding. (pnas.org)
  • The anatomical distribution of agonist-stimulated GTP[gamma-35S] binding qualitatively paralleled receptor distribution as determined by receptor binding autoradiography. (pnas.org)
  • beta-Adrenergic receptor subtypes were localized and differentiated in rat kidney slices by in vitro autoradiography using the nonselective beta-antagonist [125I]iodocyanopindolol in the presence of the selective agents betaxolol (beta 1) and zinterol (beta 2). (ahajournals.org)
  • In vitro autoradiography provides a useful means to examine different receptor populations in discrete tissue areas. (ahajournals.org)
  • Autoradiography helps to determine the distribution and density of muscarinic receptor (MR) binding sites in the brain. (cas.cz)
  • 35S]GTPgS autoradiography has facilitated the discovery of novel GPCRs, such as the P2Y12 receptor, in the nervous system. (eurekaselect.com)
  • It is evident that the integrity of the basic signalling unit, which for an increasing number of GPCRs resides in specialized membrane microdomains, is better preserved in cryostat sections than in bulk membrane preparations, suggesting that controversial issues, such as constitutive receptor activity in native brain tissue, can be rigorously addressed using [35S]GTPγS autoradiography. (eurekaselect.com)
  • Gifford Bioscience Limited is a preclinical contract research organization (CRO) providing specialist services in radioligand binding assays, receptor occupancy studies, autoradiography and SPR. (giffordbioscience.com)
  • Angiotensin II (Ang II) receptor subtypes in the rat kidney were investigated by using type 1 (AT1) and type 2 (AT2) Ang II receptor antagonists to discriminate specific 125I-[Sar1,Ile8] Ang II binding sites with in vitro autoradiography. (austin.org.au)
  • Here, we accessed side effects of recreational drugs such as ketamine, cocaine, and methamphetamine on dopamine neurons in peripheral organs by using positron emission tomography (PET) imaging and quantitative whole-body autoradiography (QWBAR) with [ 18 F]FDOPA. (hindawi.com)
  • Tissue distribution data were obtained by quantitative whole-body autoradiography. (ovid.com)
  • Angiotensin converting enzyme in the human basal forebrain and midbrain visualized by in vitro autoradiography. (biomedsearch.com)
  • Quantitative autoradiography of serotonin receptors in the rat brain. (springer.com)
  • Occupancy of central neurotransmitter receptors by risperidone, clozapine and haloperidol, measured ex vivo by quantitative autoradiography. (nih.gov)
  • Biegon A, Rainbow TC, McEwen BS (1982) Quantitative autoradiography of serotonin receptors in the rat brain. (springer.com)
  • Localization of beta 1- and beta 2-adrenergic receptors in rat kidney by autoradiography. (ahajournals.org)
  • 35S]GTPγS autoradiography is a novel functional approach to detect G protein activity in brain cryostat sections in response to stimulation of G protein-coupled receptors (GPCRs). (eurekaselect.com)
  • 35S]GTPγS autoradiography predominantly detects signalling of receptors that couple to the Gi class of G proteins, and some 20 distinct receptors belonging to this category have been studied to date under various experimental settings. (eurekaselect.com)
  • 35S]GTPγS autoradiography is particularly well-suited for functional studies on brain lysophospholipid receptors, for which conventional radioligand binding assays are not available. (eurekaselect.com)
  • In vitro autoradiography of rat and mouse spleen slices, as spleen expresses a high physiological expression of CB2 receptors, demonstrated that [ 11 C]KP23 exhibits specific binding towards CB2. (mdpi.com)
  • After functional recordings, brain was removed and processed for autoradiography (brain stem sequential slices hybridized with 35 S-oligodeoxynucleotide probes for angiotensinogen [Aogen] and angiotensin II type 1 [AT 1A ] receptors). (ahajournals.org)
  • Leukotriene receptors were examined by autoradiography, and antibody binding to the various enzymes responsible for their formation was assessed by use of immunocytochemistry. (ahajournals.org)
  • Austin Health Research Online: In vitro autoradiography reveals predominantly AT1 angiotensin II receptors in rat kidney. (austin.org.au)
  • In vitro autoradiography reveals predominantly AT1 angiotensin II receptors in rat kidney. (austin.org.au)
  • Regional 64 Cu distribution in brains was compared by ex vivo autoradiography to that of amyloid-β plaque. (rsc.org)
  • Ex vivo autoradiography, GLP-1R immunohistochemical staining, and Western blot analysis were performed to confirm the PET results. (snmjournals.org)
  • Using in-vitro autoradiography we analyze a broad range of different neuroreceptors in brain slices (10 -20 µm thick). (fz-juelich.de)
  • Methods: In vitro autoradiography were performed on SV2Alox/Cre+ transgenic mouse brain slices. (ac.be)
  • VEGFR, BrdU, lectin staining, and 125 I-VEGF 165 autoradiography on stroke brain tissue slices were performed to validate the in vivo findings. (ahajournals.org)
  • 5 edition of Positron emission tomography and autoradiography found in the catalog. (perloffphoto.com)
  • Positron Emission Tomography and Autoradiography: Principles and Applications for the Brain and Heart [Phelps, Michael E., Ph.D., Mazziotta, John, Schelbert, Heinrich Positron emission tomography and autoradiography book on *FREE* shipping on qualifying offers. (perloffphoto.com)
  • Positron Emission Tomography and Autoradiography: Principles and Applications for the Brain and Heart. (perloffphoto.com)
  • Schelbert, H R This a text on cerebral and myocardial imaging using positron emission tomography and autoradiography. (perloffphoto.com)
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  • This book Positron emission tomography and autoradiography book a must read for physicists, chemists, physicians, and advanced technologists Positron emission tomography and autoradiography book or interested in PET. (perloffphoto.com)
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  • Title:Positron Emission Tomography and Autoradiography Imaging of P-selectin Activation Using 68Ga-Fucoidan in Photothrombotic Stroke VOLUME: 15 ISSUE: 1 Author(s):Ina Israel, Felix Fluri, Anders Örbom, Fabian Schadt, Andreas K. (perloffphoto.com)
  • Manganese Imaging with Positron Emission Tomography,Autoradiography, and Magnetic ResonancebyGeoffrey John ToppingA THESIS SUBMITTED IN PARTIAL FULFILMENT OFTHE REQUIREMENTS FOR THE DEGREE OFDOCTOR OF PHILOSOPHYinThe Faculty of Graduate and Postdoctoral Studies(Physics)THE UNIVERSITY OF BRITISH COLUMBIA(Vancouver)February 2014? (ubc.ca)
  • Where to buy BioMax Autoradiography Film. (carestream.com)
  • Carestream Health, the worldwide leader in developing autoradiography films, sets the standard for research imaging with all our products, from the general purpose BioMax XAR film to the complete BioMax film family. (carestream.com)
  • Also suitable for autoradiography, but with less sensitivity for high-energy isotopes than X-Omat AR and BioMax MS, and than BioMax MR for medium-energy isotopes. (thomassci.com)
  • Measurements of glucose utilization by (14C)deoxyglucose autoradiography indicated equivalent metabolic rates for the grafted tissue and the intact substantia nigra. (osti.gov)
  • Stripping-film autoradiography of connective tissue in experimental skin tumors in mice injected intraperitoneally with S 35 , with sodium sulfate as carrier, showed that the majority of the mast cells take up sulfur. (aacrjournals.org)
  • The two hapten molecules comprising 1,4,7-triazacyclononanetriacetic acid chelate moiety (NOTA) were labeled with (67)Ga or (68)Ga. The autoradiography was conducted by incubating the tissue samples with the bispecific antibody TF2, followed by washing and incubation with one of the radiolabeled hapten molecules. (diva-portal.org)
  • CCD (charged coupled device) and CMOS imaging technologies can be applied to thin tissue autoradiography as potential imaging alternatives to using conventional film. (surrey.ac.uk)
  • The Tissue Distribution of the Haematopoetically Active Agents, Cyanocobalamin (57b12) and Inorganic Cobalt (60co), in the Rat Studied by Autoradiography. (semanticscholar.org)
  • Autoradiography (AR) can also measure quantitative distributions of Mn-52, in ex vivo brain tissue, with spatial resolution similar to MRI. (ubc.ca)
  • Site dedicated to scientific imaging systems and autoradiography products. (currentprotocols.com)
  • In summary, we have developed the first known autoradiography tool for imaging class I HDAC enzymes. (harvard.edu)
  • 2006) Digital autoradiography using CCD and CMOS imaging technology In: 15th International Workshop on Room-Temperature Semiconductor X- and Gamma-Ray Detectors/ 2006 IEEE Nuclear Science Symposium, 2006-10-29 - 2006-11-04, San Diego, CA. (surrey.ac.uk)
  • Phosphor imaging is a type of digital autoradiography that maps the distribution of U and Th in rocks and minerals. (columbia.edu)
  • I am curious, though, whether or not I can resolve these proteins by SDS/PAGE, transfer to nitrocellulose or PVDF and then detect the labelled proteins by autoradiography. (bio.net)
  • en] Background: SV2A is the most studied isoform of the Synaptic Vesicle 2 proteins, which are involved in the synaptic vesicle trafficking. (ac.be)
  • Elekes, K 1978, ' Autoradiography of monoamine uptake in Molluscan ganglia ', Neuroscience Letters , vol. 10, no. suppl. (elsevier.com)
  • Autoradiography showed the same trend of 18 F-FBEM-Cys 40 -exendin-4 uptake in the myocardial infarcted/ischemic area. (snmjournals.org)
  • Agar Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions suitable for Autoradiography studies and the detection of radioactively labelled samples. (agarscientific.com)
  • This product may be an accessory for KODAK X-OMAT AR (XAR) Autoradiography Film, Carestream Health and may differ from the image shown. (egeneralmedical.com)
  • The distribution of functionally active monoamine oxidase type A (MAO-A) was investigated by in vivo quantitative autoradiography using [ 14 C]clorgyline in normal, conscious rat brain. (springer.com)
  • Visualization of brain monoamine oxidase B (MAO-B) in dementia of Alzheimer's type by means of large cryosection autoradiography: a pilot study. (curehunter.com)
  • The anatomical distribution of the radionuclide was visualized using autoradiography at predefined time points. (nih.gov)
  • A high-performance, all-purpose autoradiography film that provides maximum sensitivity. (carestream.com)
  • The surfaces of crushed salt /NaCl/ grains were equilibrated with 36 Cl − to produce a radioactive rim that was imaged by autoradiography. (springer.com)
  • 3H]CI-994, a radioactive isotopologue of the benzamide CI-994, a class I histone deacetylase inhibitor (HDACi), was evaluated as an autoradiography probe for ex vivo labeling and localizing of class I HDAC (isoforms 1-3) in the rodent brain. (harvard.edu)
  • Autoradiography can be used to detect, and measure semiquantitatively, the radioactive materials in almost any object that can be placed in contact with film or photographic emulsion in some form. (thefreedictionary.com)
  • The photographic image of the distribution of radioactive substances in the observed object obtained through autoradiography is called an autoradiogram or radioautograph. (thefreedictionary.com)
  • Autoradiography can help establish the presence of radioactive elements in various ores, the distribution of natural radioactive elements in tissues of plant and animal organisms, and so on. (thefreedictionary.com)
  • She was a pioneer of the use of radioactive isotopes in biology and medicine, notably the techniques of radiocarbon dating and autoradiography. (wikipedia.org)
  • Autoradiography of Water-Soluble Substances A. PRECIPITATION M E T H O D In order to prevent the leaching of radioactive water-soluble substances, 32 several methods have been used in autoradiography. (walkingreadinggroup.org)
  • An increase was also observed in the migration of epithelial cells along the villus, as revealed by [3H]thymidine incorporation and autoradiography in rats fed 900 ppm Sn2+ for 4 weeks. (tudelft.nl)
  • In vitro autoradiography of hippocampal excitatory amino acid binding in aged Fischer 344 rats: relationship to performance on the Morris water maze. (oregonstate.edu)
  • Functional activity of substantia nigra grafts reinnervating the striatum: neurotransmitter metabolism and (14C)2-deoxy-D-glucose autoradiography. (osti.gov)
  • Rapidly remove haze and artifacts from X-ray film to correct overexposed blots, whether from chemiluminescent Western blot or DNA or RNA autoradiography. (fishersci.com)
  • An assemblage useful for autoradiography comprising, in order, a sample layer containing a radioactively labeled biological sample in contact with a phosphor layer, a film layer and a reflector layer. (google.com.au)
  • Advances in autoradiography: A new film brings quality improvements. (currentprotocols.com)
  • A general purpose autoradiography film for use with all commonly used isotopes and chemiluminescent labels. (carestream.com)
  • BluLite™ is a high quality, blue sensitive film that is ideal for a wide range of electrophoretic and autoradiography applications. (thomassci.com)
  • Synonym autoradiography film, xray film Double-emulsion film specifically engineered for biotechnology applications. (thomassci.com)
  • The Autoradiography Ruler Set (ARS) is an orientation and measurement device that exposes metric ruler markings directly onto x-ray film. (thomassci.com)
  • Synonym: autoradiography film, xray film General Description Coated with emulsion on both sides of the clear base. (thomassci.com)
  • KODAK X-OMAT AR (XAR) Autoradiography Film - XAR-5 Film (Alternate Interleaved) : General-purpose film for use with commonly used isotopes and chemiluminescence. (egeneralmedical.com)
  • ery versatile for autoradiography with high or low energy isotopes. (thomassci.com)
  • 14)C]2-deoxyglucose (2-DG) autoradiography was combined with T(2)(*) OC to determine metabolic status of T(2)(*)-defined penumbra. (sinapse.ac.uk)
  • Sixty minutes after [ 14 C]clorgyline administration (1.58 MBq/animal i.v.), the brains were removed and prepared for autoradiography by washing the brain sections with 5% trichloroacetic acid solution to remove the nonbinding free tracer. (springer.com)
  • Quantitative autoradiography, using 3H-deprenyl, was applied to study the regional localization of MAO-B in large cryosections from 3 control brains and 3 brains from patients with Alzheimer's disease (AD). (curehunter.com)
  • In this study, angiotensin converting enzyme was mapped in the human basal forebrain and midbrain by using quantitative in vitro autoradiography employing a derivative of a potent converting enzyme inhibitor, 125I-351A, as radioligand. (biomedsearch.com)
  • Autoradiography is widely used in physics, technology, biology, and medicine-in all cases where isotopic indicators are used. (thefreedictionary.com)
  • Baskerville, T. , McCabe, C. , Weir, C.J. , Macrae, I.M. and Holmes, W. (2012) Noninvasive MRI measurement of CBF: evaluating an arterial spin labelling sequence with 99m Tc- HMPAO CBF autoradiography in a rat stroke model. (gla.ac.uk)
  • The pCASL and 99mTc-HMPAO autoradiography CBF measures, relative to the contralateral hemisphere, were in good agreement across the spectrum of flow values in normal and ischemic tissues. (gla.ac.uk)
  • This overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial distribution of radioisotope‐labeled substances within a membrane or specimen material. (currentprotocols.com)
  • Here, a pseudo-continuous ASL technique (pCASL) was evaluated against 99mTc-D, L-hexamethylpropyleneamine oxime (99mTc-HMPAO) autoradiography in a rat stroke model. (gla.ac.uk)
  • 1. Binding sites for calcitonin-gene-related peptide were localized and characterized in porcine coronary arteries using quantitative autoradiography, and the density of binding sites was compared between large epicardial and small intramyocardial coronary arteries. (clinsci.org)
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  • Autoradiography with 3H-QNB showed genotype specific loss of binding sites. (cas.cz)
  • High-resolution autoradiography identified specific [ 3 H]-LTC 4 binding sites to smooth muscle cell and to regions of intimal proliferation and plaque. (ahajournals.org)
  • Quantitative autoradiography is described as a means of calibrating experimental results. (currentprotocols.com)
  • She spent the 1947-48 academic year in the United States learning about the recently discovered techniques of radiocarbon dating and autoradiography, which she introduced to Europe. (wikipedia.org)