Autoradiography: The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)Iodine Radioisotopes: Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.TritiumTissue Distribution: Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.Carbon Radioisotopes: Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.ThymidineBrain: The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Rats, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.Radioligand Assay: Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).Antipyrine: An analgesic and antipyretic that has been given by mouth and as ear drops. Antipyrine is often used in testing the effects of other drugs or diseases on drug-metabolizing enzymes in the liver. (From Martindale, The Extra Pharmacopoeia, 30th ed, p29)Radiopharmaceuticals: Compounds that are used in medicine as sources of radiation for radiotherapy and for diagnostic purposes. They have numerous uses in research and industry. (Martindale, The Extra Pharmacopoeia, 30th ed, p1161)Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Receptors, Neurotransmitter: Cell surface receptors that bind signalling molecules released by neurons and convert these signals into intracellular changes influencing the behavior of cells. Neurotransmitter is used here in its most general sense, including not only messengers that act to regulate ion channels, but also those which act on second messenger systems and those which may act at a distance from their release sites. Included are receptors for neuromodulators, neuroregulators, neuromediators, and neurohumors, whether or not located at synapses.Molecular Weight: The sum of the weight of all the atoms in a molecule.Binding, Competitive: The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.Radioactive Tracers: Radioactive substances added in minute amounts to the reacting elements or compounds in a chemical process and traced through the process by appropriate detection methods, e.g., Geiger counter. Compounds containing tracers are often said to be tagged or labeled. (Hawley's Condensed Chemical Dictionary, 12th ed)Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Scintillation Counting: Detection and counting of scintillations produced in a fluorescent material by ionizing radiation.Sulfur Radioisotopes: Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes.Deoxyglucose: 2-Deoxy-D-arabino-hexose. An antimetabolite of glucose with antiviral activity.Affinity Labels: Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.Fluorine Radioisotopes: Unstable isotopes of fluorine that decay or disintegrate emitting radiation. F atoms with atomic weights 17, 18, and 20-22 are radioactive fluorine isotopes.Kinetics: The rate dynamics in chemical or physical systems.Isotope Labeling: Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.Positron-Emission Tomography: An imaging technique using compounds labelled with short-lived positron-emitting radionuclides (such as carbon-11, nitrogen-13, oxygen-15 and fluorine-18) to measure cell metabolism. It has been useful in study of soft tissues such as CANCER; CARDIOVASCULAR SYSTEM; and brain. SINGLE-PHOTON EMISSION-COMPUTED TOMOGRAPHY is closely related to positron emission tomography, but uses isotopes with longer half-lives and resolution is lower.Flunitrazepam: A benzodiazepine with pharmacologic actions similar to those of DIAZEPAM that can cause ANTEROGRADE AMNESIA. Some reports indicate that it is used as a date rape drug and suggest that it may precipitate violent behavior. The United States Government has banned the importation of this drug.Densitometry: The measurement of the density of a material by measuring the amount of light or radiation passing through (or absorbed by) the material.Copper Radioisotopes: Unstable isotopes of copper that decay or disintegrate emitting radiation. Cu atoms with atomic weights 58-62, 64, and 66-68 are radioactive copper isotopes.Dimethadione: An anticonvulsant that is the active metabolite of TRIMETHADIONE.Benperidol: A butyrophenone with general properties similar to those of HALOPERIDOL. It has been used in the treatment of aberrant sexual behavior. (From Martindale, The Extra Pharmacopoeia, 30th ed, p567)Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Receptors, Dopamine D2: A subfamily of G-PROTEIN-COUPLED RECEPTORS that bind the neurotransmitter DOPAMINE and modulate its effects. D2-class receptor genes contain INTRONS, and the receptors inhibit ADENYLYL CYCLASES.NitroimidazolesRadiochemistry: The study of the chemical and physical phenomena of radioactive substances.Iodocyanopindolol: A highly selective and specific beta antagonist that is used to characterize beta-adrenoceptors.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Receptors, Somatostatin: Cell surface proteins that bind somatostatin and trigger intracellular changes which influence the behavior of cells. Somatostatin is a hypothalamic hormone, a pancreatic hormone, and a central and peripheral neurotransmitter. Activated somatostatin receptors on pituitary cells inhibit the release of growth hormone; those on endocrine and gastrointestinal cells regulate the absorption and utilization of nutrients; and those on neurons mediate somatostatin's role as a neurotransmitter.Time Factors: Elements of limited time intervals, contributing to particular results or situations.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Brain Chemistry: Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states.Receptors, Cell Surface: Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.Receptors, Angiotensin: Cell surface proteins that bind ANGIOTENSINS and trigger intracellular changes influencing the behavior of cells.Receptors, Dopamine: Cell-surface proteins that bind dopamine with high affinity and trigger intracellular changes influencing the behavior of cells.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Iofetamine: An amphetamine analog that is rapidly taken up by the lungs and from there redistributed primarily to the brain and liver. It is used in brain radionuclide scanning with I-123.Azides: Organic or inorganic compounds that contain the -N3 group.Spiperone: A spiro butyrophenone analog similar to HALOPERIDOL and other related compounds. It has been recommended in the treatment of SCHIZOPHRENIA.Radioisotopes: Isotopes that exhibit radioactivity and undergo radioactive decay. (From Grant & Hackh's Chemical Dictionary, 5th ed & McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Cerebrovascular Circulation: The circulation of blood through the BLOOD VESSELS of the BRAIN.Pindolol: A moderately lipophilic beta blocker (ADRENERGIC BETA-ANTAGONISTS). It is non-cardioselective and has intrinsic sympathomimetic actions, but little membrane-stabilizing activity. (From Martindale, The Extra Pharmocopoeia, 30th ed, p638)Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Succinimides: A subclass of IMIDES with the general structure of pyrrolidinedione. They are prepared by the distillation of ammonium succinate. They are sweet-tasting compounds that are used as chemical intermediates and plant growth stimulants.Betaxolol: A cardioselective beta-1-adrenergic antagonist with no partial agonist activity.Corpus Striatum: Striped GRAY MATTER and WHITE MATTER consisting of the NEOSTRIATUM and paleostriatum (GLOBUS PALLIDUS). It is located in front of and lateral to the THALAMUS in each cerebral hemisphere. The gray substance is made up of the CAUDATE NUCLEUS and the lentiform nucleus (the latter consisting of the GLOBUS PALLIDUS and PUTAMEN). The WHITE MATTER is the INTERNAL CAPSULE.Minicomputers: Small computers that lack the speed, memory capacity, and instructional capability of the full-size computer but usually retain its programmable flexibility. They are larger, faster, and more flexible, powerful, and expensive than microcomputers.Muscimol: A neurotoxic isoxazole isolated from species of AMANITA. It is obtained by decarboxylation of IBOTENIC ACID. Muscimol is a potent agonist of GABA-A RECEPTORS and is used mainly as an experimental tool in animal and tissue studies.Misonidazole: A nitroimidazole that sensitizes normally radio-resistant hypoxic cells to radiation. It may also be directly cytotoxic to hypoxic cells and has been proposed as an antineoplastic.Technetium: The first artificially produced element and a radioactive fission product of URANIUM. Technetium has the atomic symbol Tc, atomic number 43, and atomic weight 98.91. All technetium isotopes are radioactive. Technetium 99m (m=metastable) which is the decay product of Molybdenum 99, has a half-life of about 6 hours and is used diagnostically as a radioactive imaging agent. Technetium 99 which is a decay product of technetium 99m, has a half-life of 210,000 years.Epithelium: One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.ThiosemicarbazonesAmphetamines: Analogs or derivatives of AMPHETAMINE. Many are sympathomimetics and central nervous system stimulators causing excitation, vasopressin, bronchodilation, and to varying degrees, anorexia, analepsis, nasal decongestion, and some smooth muscle relaxation.Rats, Inbred F344Phosphorus Radioisotopes: Unstable isotopes of phosphorus that decay or disintegrate emitting radiation. P atoms with atomic weights 28-34 except 31 are radioactive phosphorus isotopes.Rats, Wistar: A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.Strontium Isotopes: Stable strontium atoms that have the same atomic number as the element strontium, but differ in the atomic weight. Sr-84, 86, 87, and 88 are the stable strontium isotopes.Guanosine 5'-O-(3-Thiotriphosphate): Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.Annexin A6: Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.Organoids: An organization of cells into an organ-like structure. Organoids can be generated in culture. They are also found in certain neoplasms.Receptors, Phencyclidine: Specific sites or molecular structures on cell membranes or in cells with which phencyclidine reacts or to which it binds to elicit the specific response of the cell to phencyclidine. Studies have demonstrated the presence of multiple receptor sites for PCP. These are the PCP/sigma site, which binds both PCP and psychotomimetic opiates but not certain antipsychotics, and the PCP site, which selectively binds PCP analogs.Quinuclidinyl Benzilate: A high-affinity muscarinic antagonist commonly used as a tool in animal and tissue studies.Democratic Republic of the Congo: A republic in central Africa, east of the REPUBLIC OF THE CONGO, south of the CENTRAL AFRICAN REPUBLIC and north of ANGOLA and ZAMBIA. The capital is Kinshasa.Receptors, Vasoactive Intestinal Peptide: Cell surface proteins that bind VASOACTIVE INTESTINAL PEPTIDE; (VIP); with high affinity and trigger intracellular changes which influence the behavior of cells.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Tomography, Emission-Computed: Tomography using radioactive emissions from injected RADIONUCLIDES and computer ALGORITHMS to reconstruct an image.Receptors, Opioid: Cell membrane proteins that bind opioids and trigger intracellular changes which influence the behavior of cells. The endogenous ligands for opioid receptors in mammals include three families of peptides, the enkephalins, endorphins, and dynorphins. The receptor classes include mu, delta, and kappa receptors. Sigma receptors bind several psychoactive substances, including certain opioids, but their endogenous ligands are not known.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Metabolic Clearance Rate: Volume of biological fluid completely cleared of drug metabolites as measured in unit time. Elimination occurs as a result of metabolic processes in the kidney, liver, saliva, sweat, intestine, heart, brain, or other site.Benzazepines: Compounds with BENZENE fused to AZEPINES.Fluorodeoxyglucose F18: The compound is given by intravenous injection to do POSITRON-EMISSION TOMOGRAPHY for the assessment of cerebral and myocardial glucose metabolism in various physiological or pathological states including stroke and myocardial ischemia. It is also employed for the detection of malignant tumors including those of the brain, liver, and thyroid gland. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1162)Cerebral Cortex: The thin layer of GRAY MATTER on the surface of the CEREBRAL HEMISPHERES that develops from the TELENCEPHALON and folds into gyri and sulchi. It reaches its highest development in humans and is responsible for intellectual faculties and higher mental functions.Receptors, Serotonin, 5-HT1: A subclass of G-protein coupled SEROTONIN receptors that couple preferentially to GI-GO G-PROTEINS resulting in decreased intracellular CYCLIC AMP levels.Receptors, Serotonin: Cell-surface proteins that bind SEROTONIN and trigger intracellular changes which influence the behavior of cells. Several types of serotonin receptors have been recognized which differ in their pharmacology, molecular biology, and mode of action.PhotochemistryKidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Neoplasms, Experimental: Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.Chromatography, Thin Layer: Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Bungarotoxins: Neurotoxic proteins from the venom of the banded or Formosan krait (Bungarus multicinctus, an elapid snake). alpha-Bungarotoxin blocks nicotinic acetylcholine receptors and has been used to isolate and study them; beta- and gamma-bungarotoxins act presynaptically causing acetylcholine release and depletion. Both alpha and beta forms have been characterized, the alpha being similar to the large, long or Type II neurotoxins from other elapid venoms.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Triturus: A genus of aquatic newts in the Salamandridae family. During breeding season many Triturus males have a dorsal crest which also serves as an accessory respiratory organ. One of the common Triturus species is Triturus cristatus (crested newt).Activation Analysis: A method of chemical analysis based on the detection of characteristic radionuclides following a nuclear bombardment. It is also known as radioactivity analysis. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Leucine: An essential branched-chain amino acid important for hemoglobin formation.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Diazonium CompoundsCattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Axonal Transport: The directed transport of ORGANELLES and molecules along nerve cell AXONS. Transport can be anterograde (from the cell body) or retrograde (toward the cell body). (Alberts et al., Molecular Biology of the Cell, 3d ed, pG3)Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Organometallic Compounds: A class of compounds of the type R-M, where a C atom is joined directly to any other element except H, C, N, O, F, Cl, Br, I, or At. (Grant & Hackh's Chemical Dictionary, 5th ed)Receptors, Melatonin: A family of G-protein-coupled receptors that are specific for and mediate the effects of MELATONIN. Activation of melatonin receptors has been associated with decreased intracellular CYCLIC AMP and increased hydrolysis of PHOSPHOINOSITIDES.

Ambiguity of the thymidine index. (1/6999)

The observed thymidine indices of seven experimental tumor lines are compared as a function of duration of emulsion exposure. The effects of dose level of tritiated thymidine and background threshold are also evaluated. The results indicate that an arbitrary high background threshold discriminates against "lightly" labeled cells at short periods of exposure but that the chosen threshold becomes less critical with longer exposure. The observed thymidine index increases with increasing duration of emulsion exposure but appears to approach a plateau for all tumor systems. The "thymidine index curves" are significantly different for each tumor. There is an inverse relationship between the dose of tritiated thymidine and the duration of exposure required to recognize the same fraction of cells as labeled in a given tumor. Similar experimental conditions do not necessarily guarantee a valid basis for comparison of observed thymidine indices among tumors.  (+info)

Integrin subunit gene expression is regionally differentiated in adult brain. (2/6999)

Integrins are a diverse family of heterodimeric (alphabeta) adhesion receptors recently shown to be concentrated within synapses and involved in the consolidation of long-term potentiation. Whether neuronal types or anatomical systems in the adult rat brain are coded by integrin type was studied in the present experiments by mapping the relative densities of mRNAs for nine alpha and four beta subunits. Expression patterns were markedly different and in some regions complementary. General results and areas of notable labeling were as follows: alpha1-limited neuronal expression, neocortical layer V, hippocampal CA3; alpha3 and alpha5-diffuse neuronal and glial labeling, Purkinje cells, hippocampal stratum pyramidale, locus coeruleus (alpha3); alpha4- discrete limbic regions, olfactory cortical layer II, hippocampal CA2; alpha6-most prominently neuronal, neocortical subplate, endopiriform, subiculum; alpha7-discrete, all neocortical layers, hippocampal granule cells and CA3, cerebellar granule and Purkinje cells, all efferent cranial nerve nuclei; alpha8-discrete neuronal, deep cortex, hippocampal CA1, basolateral amygdala, striatum; alphaV-all cortical layers, striatum, Purkinje cells; beta4-dentate gyrus granule cells; beta5-broadly distributed, neocortex, medial amygdala, cerebellar granule and Purkinje cells, efferent cranial nerve nuclei; alpha2, beta2, and beta3-mRNAs not detected. These results establish that brain subfields express different balances of integrin subunits and thus different integrin receptors. Such variations will determine which matrix proteins are recognized by neurons and the types of intraneuronal signaling generated by matrix binding. They also could generate important differences in synaptic plasticity across brain systems.  (+info)

Activated macrophages and microglia induce dopaminergic sprouting in the injured striatum and express brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. (3/6999)

Nigrostriatal dopaminergic neurons undergo sprouting around the margins of a striatal wound. The mechanism of this periwound sprouting has been unclear. In this study, we have examined the role played by the macrophage and microglial response that follows striatal injury. Macrophages and activated microglia quickly accumulate after injury and reach their greatest numbers in the first week. Subsequently, the number of both cell types declines rapidly in the first month and thereafter more slowly. Macrophage numbers eventually cease to decline, and a sizable group of these cells remains at the wound site and forms a long-term, highly activated resident population. This population of macrophages expresses increasing amounts of glial cell line-derived neurotrophic factor mRNA with time. Brain-derived neurotrophic factor mRNA is also expressed in and around the wound site. Production of this factor is by both activated microglia and, to a lesser extent, macrophages. The production of these potent dopaminergic neurotrophic factors occurs in a similar spatial distribution to sprouting dopaminergic fibers. Moreover, dopamine transporter-positive dopaminergic neurites can be seen growing toward and embracing hemosiderin-filled wound macrophages. The dopaminergic sprouting that accompanies striatal injury thus appears to result from neurotrophic factor secretion by activated macrophages and microglia at the wound site.  (+info)

Development and cytodifferentiation of the rabbit pars intermedia. II. Neonatal to adult. (4/6999)

Material from pars intermedia obtained from rabbits ranging from the second week post-partum to the adult stage, and including specimens from pregnant animals, was studied. The rate of cell division became greatly reduced early in postnatal) development. The commonest type of cell (the pars intermedia-glandular cell) becomes increasingly PAS-positive during the early stages of development. Although by 35 days differentiation of all the ACT-type cells is complete, the pars intermedia-glandular cells take as long as 53 days to mature. The epithelioid border of the hypophysial cleft persists throughout life, commonly containing dark cells. A ciliary fringe frequently appears in neonates and persists in pregnancy. Possible functions of such cilia are discussed. Throughout development the fine structure of the vasculature was studied. Secretory granules resembling those within the cells were seen in and around the blood vessels, and the mode of endocrine secretion in the pars intermedia tissue is discussed. The pars intermedia-glandular cells of the pregnant rabbits appeared hyperactive. The functional significance of the mammalian pars intermedia is discussed.  (+info)

Onset of nucleolar and extranucleolar transcription and expression of fibrillarin in macaque embryos developing in vitro. (5/6999)

Specific aims were to characterize the onset of nucleolar and extranucleolar transcription and expression of the nucleolar protein fibrillarin during preimplantation development in vitro in macaque embryos using autoradiographic and immunocytochemical techniques. Autoradiography was performed on whole embryos cultured with [3H]uridine for assessment of nucleolar (rRNA) and extranucleolar (mRNA) transcription. Expression of fibrillarin was immunocytochemically assessed in whole embryos using a primary antibody against fibrillarin and a fluorescein isothiocyanate-conjugated secondary antibody. Extranucleolar incorporation of [3H]uridine was first detected in 2-cell embryos cultured 6-10 h with [3H]uridine. Culture with alpha-amanitin prevented incorporation of label in 2-cell embryos, and treatment with ribonuclease reduced the signal to background levels, indicating that [3H]uridine was incorporated into mRNA and not rRNA or DNA. Nucleolar incorporation of [3H]uridine was not evident in pronucleate-stage or 2- to 5-cell embryos, but it was detected in one 6-cell embryo and in all 8-cell to blastocyst-stage embryos. Fibrillarin was first expressed in some 6- to 7-cell embryos, but it was consistently expressed in all 8-cell embryos. Fibrillarin was localized to the perimeter of the nucleolar precursor bodies, forming a ring that completely encapsulated these structures. Fibrillarin was not expressed in 8- to 16-cell embryos cultured with alpha-amanitin, indicating that it is transcribed, rather than recruited, at the 8-cell stage. In conclusion, in in vitro-fertilized macaque embryos developing in vitro, extranucleolar synthesis of mRNA is initiated at the 2-cell stage while the onset of nucleolar transcription occurs at the 6- to 8-cell stage, coincident with expression of fibrillarin.  (+info)

The cerebral metabolic consequences of nitric oxide synthase deficiency: glucose utilization in endothelial and neuronal nitric oxide synthase null mice. (6/6999)

Nitric oxide has multiple physiologic roles in the CNS. Inhibiting nitric oxide synthesis might therefore alter functional activity within the brain. We used [14C]-2-deoxyglucose in vivo autoradiography to measure local CMRglc in "knockout" mice lacking the genes for either the endothelial (eNOS) or neuronal (nNOS) isoforms of nitric oxide synthase, and in the progenitor strains (SV129, C57B1/6). Glucose utilization levels did not significantly differ between nNOS and eNOS knockout mice and C57B1/6 mice in any of the 48 brain regions examined, but were relatively lower in some subcortical regions in SV129 mice.  (+info)

Age-related reductions in [3H]WIN 35,428 binding to the dopamine transporter in nigrostriatal and mesolimbic brain regions of the fischer 344 rat. (7/6999)

In the present study, we used the potent cocaine analog [3H]WIN 35, 428 to map and quantify binding to the dopamine transporter (DAT) within the dorsal striatum, nucleus accumbens, substantia nigra, and ventral tegmental area in young (6-month-old), middle-aged (12-month-old), and aged (18- and 24-month-old) Fischer 344 rats. Quantitative autoradiographic analysis of indirect [3H]WIN 35,428 saturation curves revealed two-site binding for all four brain regions in every age group. The percentage of binding to the high- or low-affinity sites did not differ with age or region and was approximately 50%. However, significant age-related decreases in the overall density (Bmax) of [3H]WIN 35,428-binding sites were observed in the striatum, nucleus accumbens, substantia nigra, and ventral tegmental area. The Bmax within all brain regions declined by more than 15% every 6 months, with the Bmax in the aged (24-month-old) group being approximately half that measured in the young adult (6-month-old) group. Competition experiments indicated that nomifensine also exhibited two-site binding to the DAT in Fischer 344 rats. No consistent age-related differences in binding affinities were noted with either [3H]WIN 35,428 or nomifensine. Taken together, these results support the hypothesis that functional DATs within the nigrostriatal and mesolimbic systems are down-regulated with age, without changing their affinity for ligands.  (+info)

Effect of diabetes and aminoguanidine therapy on renal advanced glycation end-product binding. (8/6999)

BACKGROUND: Advanced glycation end-products (AGEs) have been implicated in the pathogenesis of diabetic nephropathy, and aminoguanidine (AG) has been shown to decrease the accumulation of AGEs in the diabetic kidney. METHODS: This study investigates changes in AGE binding associated with diabetes in the rat kidney using in vitro and in vivo autoradiographic techniques. Male Sprague-Dawley rats were randomized into control and diabetic groups with and without AG treatment and were sacrificed after three weeks. Frozen kidney sections (20 microm) were incubated with [125I]-AGE-RNase or [125I]-AGE-BSA. To localize the AGE binding site, in vivo autoradiography was performed by injection of 15 microCi of [125I]-AGE-BSA into the abdominal aorta of the rat. RESULTS: Low-affinity binding sites specific for AGEs in the renal cortex (IC50 = 0.28 microm) were detected by in vitro autoradiography. There was a significant increase in [125I]-AGE binding in the diabetic kidney, which was prevented by AG treatment. Emulsion autoradiography revealed that binding was localized primarily to proximal tubules in the renal cortex. Renal AGE levels, as assessed by fluorescence or by radioimmunoassay, were increased after three weeks of diabetes. This increase was attenuated by AG therapy. CONCLUSIONS: AGE binding sites are present within the proximal tubules of the kidney and appear to be modulated by endogenous AGE levels. It remains to be determined if these binding sites represent receptors involved in clearance of AGEs or are linked to pathogenic pathways that lead to the development of diabetic nephropathy.  (+info)

*Hilde Levi

Autoradiography was then used by the Finsen Institute to investigate the effects of the radiocontrast agent thorotrast. Levi ... She developed the new technique of autoradiography while working for the United States Atomic Energy Commission at the ... Levi, Hilde; Boyd, George A. (20 January 1950). "Carbon 14 Beta Track Autoradiography". Science. 111 (2873): 58-59. Bibcode: ... notably the techniques of radiocarbon dating and autoradiography. In later life she became a scientific historian, and ...

*Polyacrylamide gel electrophoresis

Autoradiography, also used for protein band detection post gel electrophoresis, uses radioactive isotopes to label proteins, ... most commonly with Coomassie Brilliant Blue R-250 or autoradiography; for nucleic acids, ethidium bromide; or for either, ... and can be digested to extract separated fractions or dried for autoradiography and permanent recording. ...

*Wolfgang Klietmann

Rohr, HP; Klietmann, W; Oehlert, W (1965). "Possibilities of the use of electron microscopic autoradiography". Zeitschrift für ...

*PDE4C

... rolipram binding autoradiography. Comparison with monkey and rat brain". J. Chem. Neuroanat. 20 (3-4): 349-74. doi:10.1016/ ...

*Michael Kaplan (biologist)

They used tritiated thymidine autoradiography to label the cells. Tritiated thymidine is incorporated into the DNA of dividing ... since no phenotypic markers were available that could be used in conjunction with thymidine autoradiography. The absence of ...

*MBF Bioscience

Densita allows researchers to analyze autoradiography and optical density. https://sbir.nih.gov/statistics/success-stories/mbf- ...

*Posterior cingulate cortex

... and GABAergic receptor binding have been found with post-mortem autoradiography of schizophrenics.[28] Abnormalities in the ...

*Peptide microarray

Other label-dependent detection methods includes chemiluminescence, colorimetric or autoradiography. Label-dependent assays are ...

*Jacob Hooker

CI-994 autoradiography". Epigenetics. 8 (7): 756-764. doi:10.4161/epi.25202. ISSN 1559-2308. PMC 3781195 . PMID 23803584. ...

*Nuclease protection assay

Thus the surviving probe-mRNA complement is simply detected by autoradiography. Nuclease protection assays are used to map ...

*5-HT1A receptor

Labeled with tritium, WAY-100,635 may also be used in autoradiography. 2C-B 2C-E 2C-T-2 4C-T-2 5-CT 5-MT 5-MeO-DiPT 5-MeO-DMT 5 ... Burnet PW, Eastwood SL, Harrison PJ (1997). "[3H]WAY-100635 for 5-HT1A receptor autoradiography in human brain: a comparison ...

*Peter J. Barnes (respiratory scientist)

He was the first to map the distribution of receptors in the lung using radioligand autoradiography. His group investigated the ... Localization of ß-adrenoceptors in mammalian lung by light microscopic autoradiography. Nature 1982; 299:444-447. PMID 6289123 ...

*WAY-100,635

Labeled with tritium WAY-100,635 may also be used in autoradiography. WAY-100,635 has higher 5-HT1A affinity than 8-OH-DPAT. ... WAY-100635 for 5-HT1A receptor autoradiography in human brain: a comparison with [3H]8-OH-DPAT and demonstration of increased ...

*Plaque hybridization

This is followed by autoradiography to detect the position of the label. The plaque hybridization procedure has some advantages ...

*Non-helical models of DNA structure

"The bacterial chromosome and its manner of replication as seen by autoradiography". J. Mol. Biol. 6: 208-13. doi:10.1016/s0022- ...

*Genomic library

The target DNA- insert of interest- can be identified by detection such as autoradiography because of the hybridization with ...

*Lymphoma

Jenkins EC (Jan 1972). "Wire-loop application of liquid emulsion to slides for autoradiography in light microscopy". Stain ...

*Gelatin silver process

It is known for being nearly as sensitive as autoradiography, the "gold standard" technique, but one not widely used due to the ...

*Indorenate

... an autoradiography study". The Journal of Pharmacy and Pharmacology. 58 (9): 1243-8. doi:10.1211/jpp.58.9.0011. PMID 16945183. ...

*Genomics

... and visualised using autoradiography. The procedure could sequence up to 80 nucleotides in one go and was a big improvement, ...

*Nicolas Bazan

... docosahexaenoic acid trafficking through photoreceptors and retinal pigment epithelium by electron microscope autoradiography. ...

*Thyrotoxic myopathy

Experimental thyrotoxic myopathy - autoradiography of protein-synthesis in skeletal-muscle and motor-neurons of spinal-cord. ...

*Nuclear dots

Cytochemistry and autoradiography during stimulation by concanavalin A". Biol. Cell. 49 (1): 35-43. doi:10.1111/j.1768-322x. ...

*Nifene

... autoradiography, and behavior". Synapse. 66 (5): 418-34. doi:10.1002/syn.21530. PMC 3292694 . PMID 22213342. V, Galitovskiy; SA ...

*Immunostaining

Alternatively, radioactive elements can be used as labels, and the immunoreaction can be visualized by autoradiography. Tissue ...

*Dentate gyrus

... cytogenesis and morphogenesis examined with autoradiography and low-level X-irradiation". The Journal of Comparative Neurology ...
After monolayer cultures of rat islets were exposed to [125I]insulin,[125I]glucagon, and [125I]tyrosinyl somatostatin, specific autoradiographic grains associated with each radioactively labeled ligand were found on B, A, and D cells.The density of labeling of the B, A, and D cells with each labeled ligand correlated well with the known actions of the three hormones on each of the islet cells. ...
Many physiological mechanisms as well as numerous neurological diseases involve the complex interaction of various receptors. Quantitative evaluation of the receptor distribution is essential for developing improved procedures in diagnosis and effective treatments of neurological diseases.. Quantitative Receptor Autoradiography. Using in-vitro autoradiography we analyze a broad range of different neuroreceptors in brain slices (10 -20 µm thick). After preincubation in buffer to remove endogenous ligand, sections are incubated with a radioactive substance, that binds specificly to the receptor being studied (radioligand). For the assessment of non-specific binding, selected slices are incubated with the radioligand in presence of an specific antagonist. After washing and a rapid rinse in ice-cold water the sections are dried and exposed against phosphor-imaging plates or conventional film slides in combination with tritium activity standards (see figure 1) for quantitative evaluation. Specific ...
An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance. Alternatively, the autoradiograph is also available as a digital image (digital autoradiography), due to the recent development of scintillation gas detectors or rare earth phosphorimaging systems. The film or emulsion is apposed to the labeled tissue section to obtain the autoradiograph (also called an autoradiogram). The auto- prefix indicates that the radioactive substance is within the sample, as distinguished from the case of historadiography or microradiography, in which the sample is X-rayed using an external source. Some autoradiographs can be examined microscopically for localization of silver grains (such as on the interiors or exteriors of cells or organelles) in which the process is termed micro-autoradiography. For example, micro-autoradiography was used to examine whether atrazine was being ...
Various techniques for detection of blood platelets in tissue section were evaluated in an incremental air embolism model of ischemia in order to further investigate the accumulation of platelets in brains subjected to cell-damaging ischemia. The experimental animals were dogs anesthetized with alpha-chloralose. A new autoradiographic technique was devised that allows precise localization of 111Indium-labeled platelets in brain sections. The technique is described in detail along with some effects of the label on platelet function and behavior. The technique can be performed in conjunction with 14C-iodoantipyrine autoradiographic measurement of blood flow without causing mutual interference or other forms of interaction. This permits the simultaneous investigation of local blood flow and deposition of platelets. ...
Double-tracer autoradiographic study of protein synthesis and glucose consumption in rats with focal cerebral ischemia. - T Christensen, T Balchen, T Bruhn, N H Diemer
We have investigated 5-HT1A (serotonin1A) and 5-HT2A (serotonin2A) receptor mRNA abundance and binding site densities in various neocortical and hippocampal regions of schizophrenics and control subjects. Age, agonal state (brain pH), and post mortem interval were included where necessary as covariates in our analyses. In schizophrenics, 5-HT1A binding site densities, determined autoradiographically by [3H]8-hydroxy-2,3-(dipropylamino)-tetralin ([3H]8-OH-DPAT), were significantly increased (+23%) in the dorsolateral prefrontal cortex, with a similar trend in anterior cingulate gyrus. These increases were not accompanied by any change in 5-HT1A receptor mRNA. No differences between the groups in [3H]8-OH-DPAT binding or 5-HT1A receptor mRNA were seen in superior temporal gyrus, striate cortex, or hippocampus. 5-HT2A binding sites, determined by [3H]ketanserin, were decreased in the dorsolateral prefrontal cortex (-27%) and parahippocampal gyrus (-38%) of schizophrenics, with a similar trend in cingulate
Blanks, J C. and Bok, D, "An autoradiographic analysis of postnatal cell proliferation in the normal and degenerative mouse retina." (1977). Subject Strain Bibliography 1977. 3447 ...
Mendelsohn, M L., "Autoradiographic analysis of cell proliferation in spontaneous breast cancer of c3h mouse. Iii. The growth factor." (1961). Subject Strain Bibliography 1961. 685 ...
Unmodified oligodeoxynucleotides (ODNs) were synthesized and tested for their ability to cross external eukaryotic cell membranes and to enter the cytosol and nucleus in tissue cultures. The ODNs were labeled with high-specific-activity [3H]thymidine (| or = 100 Ci/mmol), or [ alpha-32P]ATP or [ gamma-32P]ATP (300-1000 Ci/mmol; 1 Ci = 37 GBq), and the label was either in the central portion of the molecule or at the 3 or 5 end. The cells employed were for the most part 3T6 murine fibroblasts, grown in monolayers, either semiconfluent or confluent, but some experiments were carried out with chicken embryo fibroblasts or human HeLa cells. Parallel wells in the same experiment were prepared for electron microscopy or for cell fractionation and radioactivity assays. Electron microscopic autoradiography indicated that ODNs cross the external cell membrane, traverse the cytosol, and begin to enter the cell nucleus within a few seconds to 5 min at 37 degrees C in Dulbeccos medium without added serum. After
CCD (charged coupled device) and CMOS imaging technologies can be applied to thin tissue autoradiography as potential imaging alternatives to using conventional film. In this work, we compare two particular devices: a CCD operating in slow scan mode and a CMOS-based active pixel sensor, operating at near video rates. Both imaging sensors have been operated at room temperature using direct irradiation with images produced from calibrated microscales and radiolabelled tissue samples. We also compare these digital image sensor technologies with the use of conventional film. We show comparative results obtained with (14)C calibrated microscales and (35)S radiolabelled tissue sections. We also present the first results of (3)H images produced under direct irradiation of a CCD sensor operating at room temperature. Compared to film, silicon-based imaging technologies exhibit enhanced sensitivity, dynamic range and linearity.. ...
Regional measurements of 131I-9.2.27 distribution in human melanoma tumors were obtained using quantitative autoradiography. Tumors were removed from patients 72-96 h after they had received an i.v. injection of 9.15 mCi (100 mg) of 131I-9.2.27. The autoradiographic images showed that the radioactivity reaching the tumor was heterogeneously distributed. Areas of relative high and low uptake were selected in each tumor. Regions of high activity contained from 51 to 1371 nCi/g, while areas with low uptake had radioactivity ranging from 12 to 487 nCi/g. The reliability of the autoradiographic measurements was demonstrated by the strong positive correlation with direct tissue sample counting (r = 0.994 P , 0.001). Since comparative immunocytochemistry showed a homogeneous and diffuse staining of target antigen on viable tumor cells, variability of monoclonal antibody uptake within individual tumors was not primarily due to heterogeneity of antigen expression in these cases. However, antigen levels ...
The presence and abundance of 5-HT1A and 5-HT2A receptor mRNAs in post mortem human hippocampus was investigated using a novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) technique using cyclophilin mRNA as an internal standard. 5-HT1A and 5-HT2A receptor mRNAs were each co-amplified with varying dilutions of cyclophilin primers, and their abundance expressed as a ratio of cyclophilin mRNA. Using this technique in combination with quantitative autoradiography we have investigated the effect of aging on hippocampal 5-HT1A and 5-HT2A receptor mRNA abundance and binding site densities. There was a significant negative correlation between hippocampal 5-HT1A receptor binding site densities and age and a similar trend for 5-HT1A receptor mRNA abundance. Neither 5-HT2A receptor binding site densities nor mRNA abundance were affected by age. Both 5-HT1A and 5-HT2A receptor binding site densities in individual subjects correlated significantly with abundance of their encoding mRNA. This
Distribution of 2-chloro-2 -deoxyadenosine, 2-chloro-2 -arabino-fluoro-2 -deoxyadenosine, Fludarabine and Cytarabine in mice: a whole-body autoradiography study ...
Ling, E.A. (1974). Ultrastructure and radioautographic studies of the thyroid gland. Annals of the Academy of Medicine Singapore 3 (3) : 207-211. [email protected] Repository ...
Morphological analysis of transplantable pancreatic acinar carcinoma of rat has revealed a continuum of cells from those which totally lack mature secretory granules to cells with abundant well-formed secretory granules. This tumor is easily dissociable into single cells which incorporate [3H]thymidine and [3H]leucine into DNA and protein at a linear rate for 3 to 4 hr. The number of cells synthesizing DNA, as assessed by light microscope autoradiography, was about 17%. Dissociated neoplastic pancreatic acinar cells are classified into four cell types (types I to IV) on the basis of nuclear morphology, degree of polarization, and the extent of specialization of cytoplasmic organelles. Type IV cells possess all the characteristics of mature pancreatic acinar cells, such as abundant rough endoplasmic reticulum, prominent Golgi apparatus, and numerous secretory granules. Cell types III, II, and I are progressively less differentiated. An analysis of the cytological features of 285 dissociated tumor ...
Agar Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions suitable for Autoradiography studies and the detection of radioactively labelled samples.
The distribution of infused tritiated norepinephrine (NE-3H) in small mesenteric arteries and intestinal arterioles in rats was investigated with electron microscopic radioautography. Silver grains, indicating the presence of the tritium label on the sections, were found lying mainly over axon bundles, but some were present over collagen and smooth muscle cells. Axons with the highest concentrations of silver grains had been sectioned at points where they were naked of Schwann cell sheath, were dilated into varicosities, and contained small granular vesicles. This finding was taken as confirmatory circumstantial evidence that the small granular vesicles were the sites of uptake and storage of NE. The short interval between the start of infusion and the fixation of the tissue appeared to rule out any process other than a direct uptake of NE by the peripheral axons. If axonal sites of uptake of NE-3H correspond to sites of release of NE, then the evidence suggests that such sites of release are ...
Conceptual computer illustration of the DNA double helix together with a graphic representation of an autoradiograph display. The pattern of the DNA autoradiograph bands is unique to each individual, but some bands are shared by related people, such as a parent & child. DNA fingerprints can be used to prove conclusively whether people are related. - Stock Image C010/5265
The distribution of cells expressing mu-receptor mRNA and mu-receptor binding sites were compared in brain and spinal cord tissue sections using a combination of in situ hybridization and receptor autoradiographic techniques. mu-Receptor mRNA was visualized with a 35S-labeled cRNA probe directed to transmembrane III-VI of the rat mu-receptor, while mu-receptor binding sites were labeled with the mu-selective ligand [3H]DAMGO ...
The IVISR provides optical, CT, ultrasound, high-resolution planar gamma imaging, and PET of mice and rats, as well as high-resolution digital autoradiography and fluorescent imaging of thin whole body rodent sections.
Figure 1 Antiphospho-Pak1 antibody specifically recognizes activated Pak1. (A) Activation loop sequence of the phosphorylated peptide used for production of polyclonal antiphospho-Pak1 antisera. (B) Immunoblots (lanes 1-4) and radiolabeled kinase reaction products (lanes 5 and 6) of baculovirus-produced Pak1. Purified KD or CA Pak1 was incubated in protein kinase buffer in the presence of ATP, then immunoblotted using anti-Pak1 (lanes 1 and 2) and antiphospho-Pak antisera (lanes 3 and 4) autoradiographed to detect antibody-bound proteins. Although both forms of the protein were detected with anti-Pak1, only activated Pak1 was detected using the phospho-specific antibody, despite the large amount of recombinant protein used in this assay. Lanes 5 and 6 show autoradiographed PAGE gel of reaction products from a kinase assay performed in the presence of [γ-32P]ATP. Molecular mass in kilodaltons is shown to the right of the blot. (C) Comparison between immunoblot and radiolabeled kinase reaction ...
PCR products were separated by electrophoresis (Protean-2, Biorad, Issy-les-Moulineaux, France) on 8% polyacrylamide gels, which were then vacuum dried and processed for autoradiography on Biomax films (Kodak, Sevran, France). Exposure times were adjusted to avoid film saturation. For each primer pair, we first determined the optimal hybridization temperature and minimum number of PCR cycles necessary to detect radiolabeled reaction products at a unique, expected molecular size, so that each PCR analysis would be performed in the exponential phase of amplification. Forward and reverse primers were designed in separate exons to avoid any bias caused by residual genomic contamination. PCR-amplified products were checked with restriction enzymes. For all primer pairs, no amplification was observed when PCR was performed on either RNase-free water or non-reverse-transcribed RNA samples. At least three independent PCRs were performed on the same samples for each primer pair.. Optical densities (ODs) ...
The first concerns cellular mechanisms of learning and memory. There is considerable evidence that a crucial step in memory formation is the synthesis of new proteins in the hippocampus. We are using autoradiographic techniques to focus on proteins that are made in dendrites of hippocampal neurons, near synapses. Very few proteins are made there, but we have found out that electrical stimulation of the axons that make contact with these dendrites greatly stimulates their synthesis of proteins. This only happens when the stimulus is combined with another neurotransmitter, acetylcholine. There is reason to believe this phenomenon is important in memory formation and we are studying its mechanisms and significance. The second area concerns the regulation of synaptic transmission by glycolysis. We have found that synaptic transmission in the hippocampus will only occur if glycolysis is active, even if adequate energy is supplied in other ways. This is very unexpected. There appears to be a ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Ive had a decades-long fascination with herbs and natural healing and found over the years that many such treatments can be quite successful. Im...
Generate more awareness for your business with branded car magnets. These versatile magnets offer a less permanent option over decals or wraps.
Grain counting by eye is a tedious and time-consuming technique but one with great potential in cell kinetics and for the study of DNA excision repair activity (unscheduled DNA synthesis or UDS). We have been investigating the levels of UDS in human skin sections exposed in situ to ultraviolet radiation using a short-term incubation in tritiated thymidine and autoradiography and the decline in UDS levels with time (repair kinetics). We have adapted an automated image analysis system automatically to assess the number of grains over epidermal cell nuclei in autoradiographs of sections of epidermis. An excellent correlation was observed between visual counting and machine measurement of the area (in pixels) occupied by silver grains. The levels of UDS declined with time as lesions are progressively repaired. The half time (+/- standard deviation) for the reduction in UDS is 7.25 +/- 0.18 h. The grain counts can be significantly increased by increasing the autoradiographic exposure, by increasing ...
The modulation of oxytocin (OT) receptors (OTRs) by estrogen was investigated in the ventromedial hypothalamus by in vitro receptor autoradiography. Treatment of ovariectomized and adrenalectomized rats with various doses of estradiol benzoate (EB) increased OTR binding not only in the ventromedial nuclei of the hypothalamus (VMN), but also in the area lateral to the nuclei (IVMN). After a single injection of EB, OTRs first were induced within the ventrolateral parts of the VMN, and only hours later they appeared in the IVMN. This is consistent with the interpretation that OTRs are first induced within the estrogen- sensitive neurons of the ventrolateral VMN and then are transported laterally out of the nuclei. Two additional experiments confirmed this interpretation. First, local infusion of a low dose (10 micrograms) of the neuronal transport inhibitor vinblastine blocked the appearance of OTRs in the IVMN but did not prevent the induction of OTRs by EB within the nuclei. Second, a knife cut ...
The publisher regrets that a mistake was published in this article. On page 747, the word "antagonist" was incorrectly published instead of "agonist." The whole sentence should correctly read: "Importantly, also in response to an adenosine A1 agonist, old rats had less sleep compared with young animals." Our sincere apologies are offered to the authors, editor, and readers.. The original article of this erratum was published in J Neurosci Res 2004; 78: 742-748 ...
Charles River designs and conducts GLP-compliant quantitative whole-body autoradiography (QWBA) programs integrated with our preclinical drug metabolism services.
An assemblage useful for autoradiography comprising, in order, a sample layer containing a radioactively labeled biological sample in contact with a phosphor layer, a film layer and a reflector layer. The phosphor layer is preferably a gadolinium oxysulfide containing layer and the film is preferably a photographic film containing a tabular grain emulsion.
DNA. Computer artwork of a DNA (deoxyribonucleic acid) double helix seen against autoradiograms of genetic sequences (upper right). The spiralling strands of the DNA helix (blue) are composed of complex chemical groups called nucleotides, which consist of a sugar phosphate and a base group. Complementary pairing between the base groups of nucleotides on opposite strands holds the helix together. The sequence of the 4 base groups (adenine, cytosine, guanine and thymine; coloured rods) along the DNA helix is unique for every individual and is known as the genetic code. The autoradiograms depict DNA base sequences, each band (dark blue) representing an individual base. - Stock Image G110/0642
The effect of aerosol upon cloud properties is studied over the Baltic Sea region, which presents a distinct contrast of aerosol loading between the clean Fennoscandia and the polluted area of central-eastern Europe. Statistically significant positive values are found over the Baltic Sea and Fennoscandia, while negative values are found over central-eastern Europe, contradicting the theory of aerosol indirect effect on clouds ...
A variety of methods have been used in published studies for image segmentation (manual outlining, thresholding, magic wand, use of templates, etc.) to determine what is part of the structure or region of interest and what is not. Similarly, once grey levels are measured, a variety of methods have been employed to convert them into optical densities or units of radioactivity. Based on the above results, the line method was the best way to select the structure or region of interest as it was not subject to floor-effects, had a low coefficient of variation, and low within-sample variability. This method is similar to, and a modification of the outline method, which requires that the actual boundaries of the structure be determined, whereas the line method sampled only from the interior of the structure. Since the DG is a long narrow structure, this was best done with a line down the centre. Larger structures can follow the same principle by outlining the structure but staying well inside the ...
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There are 13 fifteen-letter words containing A, D, G, U and Y: AUTORADIOGRAPHY DAGUERREOTYPERS DAGUERREOTYPIES ... PSEUDOPREGNANCY RADIOAUTOGRAPHY UNDERSTANDINGLY. Every word on this site can be used while playing scrabble. Create other lists, beginning with or ending with letters of your choice.
TY - JOUR. T1 - Characteristics of muscarinic cholinergic, γ-aminobutyric acidA and phencyclidine receptors in spontaneously epileptic rats; in vitro quantitative autoradiographic analysis. AU - Murase, Kenshi. AU - Nabeshima, Toshitaka. AU - Kameyama, Tsutomu. AU - Sasa, Masashi. AU - Takaori, Shuji. AU - Ujihara, Hisamitsu. AU - Ishihara, Kumatoshi. AU - Yamada, Junzo. AU - Serikawa, Tadao. PY - 1991/9/30. Y1 - 1991/9/30. N2 - Characteristics of muscarinic cholinergic (mACh), γ-aminobutyric acidA (GABAA) and phencyclidine (PCP) receptors in the spontaneously epileptic rats (SER), which exhibit both absence-like seizures and tonic convulsion, were examined using in vitro quantitative autoradiography. Computer analysis using autoradiographic technique revealed that the amount of the specific binding of [3H]quinuclidinyl benzilate (QNB) to mACh receptors in the striatum of SER was more than that of zitter rats, not exhibiting both seizures and convulsion. However, the specific bindings of ...
Clinically effective antidepressants are thought to exert their therapeutic effects by facilitating central monoamine neurotransmission. However, recent data showing that neurokinin-1 receptor (NK1R) antagonists have antidepressant properties in both animal and clinical studies raise the possibility that classical antidepressants may also influence NK1R expression in the brain. To test this hypothesis, rats were treated with desipramine, paroxetine, venlafaxine, tranylcypromine or vehicle for 14-42 days. NK1R binding sites and mRNA were determined in a wide variety of brain areas using in situ hybridization and quantitative receptor autoradiography. In all areas examined, the abundance of NK1R binding sites was unchanged after 14 days of treatment. None of the treatments altered the number of NK1R binding sites following 42 days treatment with the exception that an increase was found in the locus coeruleus with tranylcypromine. Taken together, we report that repeated treatment with antidepressants of
1. Binding sites for calcitonin-gene-related peptide were localized and characterized in porcine coronary arteries using quantitative autoradiography, and the density of binding sites was compared between large epicardial and small intramyocardial coronary arteries.. 2. A single class of binding sites for calcitonin-gene-related peptide with a dissociation constant of 2.1 ± 0.2 nmol/l was detected in both the large and small coronary arteries. The density of specific binding sites was higher (maximum binding site density 231 ± 14 fmol/mg of protein) in the small coronary arteries than in the large epicardial coronary arteries (maximum binding site density 108 ± 5 fmol/mg of protein). β-Human calcitonin-gene-related peptide showed higher affinity than α-human calcitonin-gene-related peptide for the binding sites. Most of the specific binding sites for both peptides in the large coronary artery were localized in the intima and media.. 3. In coronary artery from patients with coronary heart ...
THE ANATOMICAL RECORD 222:191-200 (1988) Radioautographic Demonstration of Receptors for Epidermal Growth Factor in Various Cells of the Oral Cavity MOON-IL CHO, YU LIN LEE, AND PHILIAS R. GARANT Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook, New York 11794-8700 ABSTRACT Mouse iodinated epidermal growth factor (EGF)was localizedby light and electron microscopic radioautography in basal cells of oral epithelium, papillary cells of the enamel organ, periodontal ligament fibroblasts, preodontoblast precursor cells, and preosteoblasts of the alveolar bone of 13-day-old Sprague-Dawleyrats. The specificity of binding in these cells was suggested by an observed reduction of about 90% in the labeling when excess unlabeled EGF was injected along with the 125I-EGF. In contrast, fully differentiated cells, such as ameloblasts. odontoblasts, and osteoblasts, were only poorly labeled. Quantitative analysis of the light microscopic ...
Glycoproteins were metabolically labeled with 3H-fucose in cultured RPE cells from RCS rdy-p+ and Long Evans rats. 3H-labeled glycoproteins associated with a plasma membrane-enriched subcellular fraction were separated by two-dimensional gel electrophoresis. Relative incorporation of 3H-fucose into high molecular weight cell surface glycoproteins (Mr of 128,000-183,000) was measured by quantitative autoradiography and densitometry. The results of these experiments show that 3H-fucose incorporation into four glycoproteins (Mr of 183,000, 175,000, 164,000 and 156,000) was reduced by 30-50% in the dystrophic RPE as compared to the normal cells. This reduction was not due to an absence of the protein core of glycoproteins on the dystrophic RPE cell surface; when RPE cells were labeled with 3H-leucine prior to analysis, no reduction in label was found in the dystrophic RPE as compared to normal. Therefore, the results of this study suggest that the RCS rat RPE processes the oligosaccharide portion of ...
Abstract Migration and elimination of 75Se-labeled Schistosoma mansoni were studied in previously infected mice by means of autoradiography and worm recovery. As in control mice, there appeared to be little if any worm elimination in the skin of previously infected mice. In previously infected mice, there appeared to be some worm elimination in the lungs and in migration sites between the lungs and liver, though much less than would have been expected from previous studies. In two of three experiments most of the challenge worm elimination in previously infected mice, above the normal attrition level occurring in the controls, appeared to take place in the liver. In the third experiment, it appeared that all of the challenge worm elimination occurred after migration to the lungs, and at least one-third of it in the liver. It was also observed that the lung chop procedure recovered viable schistosomula much less efficiently from previously infected mice than from controls, indicating that the reduction
Figure 3. Ocular clusterin localization by immunohistochemistry and in situ analysis. Shown are sections of cornea (panels A-C, serial sections), skin/eyelid (panels D, E, serial sections), ciliary body (unfilled arrow and * in panel F), and lens (panels G, H, serial sections). Immunodetection with G7 are shown in panels B, E, F, and H; panels A, D, and G are the negative controls in which the G7 antibody was omitted. In situ hybridization of an anti-sense clusterin mRNA probe on cornea is shown in panel C. Panel I: graph illustrating the distribution of silver grain over different regions of the cornea shown in panel C, for each general region 10 independent counts within a fixed area were taken. Solid arrows indicate the interface between epithelial and stromal cells (cs) in the cornea, arrow heads indicate the area of epithelial cells in the lens.. ...
Apixaban, a potent and highly selective factor Xa inhibitor, is currently under development for treatment of arterial and venous thrombotic diseases. The distribution, metabolism, and elimination of [14C]apixaban were investigated in male, female, pregnant, and lactating rats after single oral doses. Tissue distribution of radioactivity in rats was measured using quantitative whole-body autoradiography. After a single oral administration, radioactivity distributed quickly in rats with Cmax at 1 h for most tissues. The elimination t1/2 of radioactivity in blood was 1.7 to 4.2 h. The blood area under the plasma concentration-time curve of radioactivity was similar between male and female rats and was slightly higher in pregnant rats and lower in lactating rats. The radioactivity concentration in tissues involved in elimination was greater than that in blood with the highest concentration in the gastrointestinal tract, liver, and urinary bladder/contents and lowest level in brains. In pregnant ...
Abstract The influence of the melt flow on the solidification structure is bilateral. The flow plays an important role in the solidification pattern, viathe heat transfer, grain distribution, and...
Labeled antagonists have advantages over agonists for the characterization of G protein-coupled receptors if, for example, the total number of binding sites is of interest, because the binding of antagonists is independent of the fraction of receptors coupled to the GTP-binding proteins (DeLean et al., 1980). For example, in thebeta adrenergic receptor system, high-affinity antagonists were used in receptor characterization (Brown et al., 1976).. All previously reported CRF antagonists were of too low an affinity to be used as radioligands for characterizing CRF-R1 receptors. With astressin (Gulyas et al., 1995), we now have an antagonist of sufficient affinity to make it suitable as a radioligand for the detection and characterization of both cloned and endogenous CRF receptors. Using autoradiographic techniques and radioreceptor assays, brain receptors have also been detected with oCRF* (De Souza et al., 1984) and 125I-labeled [Tyr0]oCRF (Grigoriadis and De Souza, 1988;Webster et al., ...
Since the classical paper of Leblond & Stevens (1948), much information on cell renewal in the intestinal epithelium has become available. The introduction of tritiated thymidine autoradiography has diverted attention from conspicuous lacunae which still exist in our knowledge of intestinal structure and growth. This paper attempts to fill two of these elementary gaps. The first is well defined by Fry, Lesher, Kisieleski & Sacher (1963): we still remain ignorant even of the complete three-dimensional structure of the mucosa in any one region. It seems incredible that such a problem was not attacked by the great German microanatomists of the last century, but no accurate account of the three-dimensional structure of the intestinal mucosa is known to me; the accounts in present-day textbooks of histology in the English language are superficial and even inaccurate (see, for example, Bloom & Fawcett, 1962; Ham, 1965).. ...
A sample study was performed where a designed set of primers specific to the Y chromosome from the GRCh 38 Human Genome was queried in both ThermoBLAST CE and BLAST to compare the number of extensible hits and the number of false amplicons that were detected. The results showed that ThermoBLAST CE detected 765 unique extensible hits in the Y chromosome while BLAST detected only 15. Furthermore, ThermoBLAST CE detected a total of 946 extensible hits for the forward primer design, where BLAST detected only 52 extensible hits. For the reverse primer ThermoBLAST found over 98% more hits than BLAST, and likewise for the forward primer ThermoBLAST CE found over 94% more hits than BLAST. Questions about this study can be directed to DNA Software, Inc.. ThermoBLAST CETM = Cloud Integration + Custom Genome Playlists+Genome Viewer+The Computational Capacity To Use It. ...
If checking nutritional labels is like reading Chinese, you are in good company. Nutrition is complicated and a lot of the information is contradictory. The tips youre about to read will clear up your ideas of nutrition. Try to stock up on whole grains over white flour products. Fiber and protein-rich whole grains are always... » read more. ...
Proximas Horsing Around Page is a collection of links to various equestrian Pages that I have found over the Internet, a (growing) list of Toxic Plants, some humor, and pictures of my horses, both regular-sized and minis!
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Periodical: Sokoloff, Louis, Martin Reivich, Charles Kennedy, M. H. Des Rosiers, C. S. Patlak, K. D. Pettigrew, O. Sakurada, and M. Shinohara. The [14C]Deoxyglucose Method for the Measurement of Local Cerebral Glucose Utilization: Theory, Procedure, and Normal Values in the Conscious and Anesthetized Albino Rat. Journal of Neurochemistry 28, 5 (1977): 897-916. Article. 20 Images ...
Although much work has been done on the inducing factors involved in primary embryonic induction, we are far from understanding the mechanism of the phenomenon. Information concerning the changes in the synthetic patterns in the reacting tissue, which may be related to the induction, must be accumulated before we will be able to formulate a hypothesis on the mechanism of embryonic induction at a subcellular level. The work reported in this paper represents an effort to obtain preliminary information concerning the possible changes in RNA synthesis correlated with primary embryonic induction using the autoradiographic technique.. According to earlier cytochemical data of Brachet (1942), when the prospective neural ectoderm is underlaid by the archenteric roof during gastrulation in Triturus embryos, cytoplasmic basophilia of ectodermal cells increases, while in the prospective epidermal ectoderm cytoplasmic basophilia remains relatively weak. The basophilia was interpreted by Brachet to be due to ...
Ophthalmologic manifestations commonly misdiagnosed as demyelinating events in multiple sclerosis patients. This retrospective review evaluates the success of such outpatient management, including the complications encountered. Manipulation of the blue light photoreceptor cryptochrome 2 in tomato affects vegetative development, flowering time, and fruit antioxidant content. Autoradiographic studies on 3H-fucose incorporation into manubria and its translocation to antheridial space during spermatogenesis in Chara vulgaris L. This question has been addressed by a model and a methodology that uses only very basic constituents to capture the relevant features of folding. This study demonstrates the presence of molly generic viagra a rich plexus of neuropeptide Y (NPY) immunoreactive fibers in the hypothalamus and in the intermediate lobe of the pituitary of Xenopus laevis. However, sonography is becoming the preferred modality, primarily because of the superior anatomic detail provided. A ...
Lymphopoiesis was studied in 3-month-old normal C57Bl mice and in 3-month-old C57Bl mice carrying from 12 to 48 C57Bl thymus grafts using tritiated thymidine labeling.. Thymus graft lymphopoiesis was found to be identical with that of normal thymus tissue and the presence of thymus grafts was found to have no influence on host thymus lymphopoiesis.. No evidence was found that the massive amounts of thymus graft tissue in the mice affected any parameter of host lymph node lymphopoiesis nor was any evidence detected for the migration of thymic lymphocytes from these massive deposits of thymus graft tissue either to host lymph nodes and blood or to other organs in the host animal.. It is concluded that the majority of small lymphocytes produced in the thymus and thymus graft tissue do not migrate from these tissues but die locally at the end of their intrathymic life span of 3 to 4 days.. ...
The tremendous hardness of boron carbide (B4C) is only exceeding by cubic boron nitride and diamond exceed. Boron carbide ceramics have mechanical properties similar to those of silicon carbide ceramics, but have even greater wear resistance and also offer outstanding chemical resistance to acids and bases. The combination of extremely low density (2.51 g/cm3), high mechanical strength and elastic parameters makes these ceramics particularly interesting for protective ballistic applications in defense technology. H.C. Starck provides boron carbide in industrial qualities (HD grades) with a wide particle grain distribution as well as in premium qualities (HP and HS grades) with an extremely narrow particle grain distribution ...
Debbie Dewar is a neuroscientist with a B.Sc. in Biology & Psychology from Edinburgh University and a PhD from the Institute of Psychiatry, London. She has a long-standing interest in how brain chemistry and structure are altered after acute brain injury and in chronic brain disorders with a view to understanding pathogenic mechanisms and identifying targets for therapeutic intervention. Brain imaging at a variety of different levels, from tissue sections to whole brain is used in the majority of her research.. Developing novel treatments for reducing brain damage after a stroke is a major focus of current research. This work uses experimental models of cerebral ischaemia and insulin resistance in combination with various imaging techniques including, MRI, quantitative histology, immunohistochemistry and autoradiographic measurement of cerebral blood flow to understand how hyperglycaemia caused by either brain injury-induced stress or existing insulin resistance influences the extent of brain ...
Mp3 To Ogg File Converter Free Download MP3 WAV OGG Converter - Free download and software reviews. - As you probably guessed, this ultrasimple (but unfortunately not ultracheap) program converts audio files between MP3, WAV, and OGG formats. If you need to. Also supports to convert video and audio files to vorbis ogg. The best and. Click the Download button below to. Merge MP3s into a single mp3 file in just a few clicks. Seamless Mp3 Merging, user-friendly Interface. The Best MP3 Merger to merge a bunch of MP3 files into one.. Autoradiography 5.19 PROTHIOCONAZOLE (232) TOXICOLOGY - Prothioconazole 265 5.19 PROTHIOCONAZOLE (232) TOXICOLOGY Prothioconazole is the ISO approved common name for the substance for which the IUPAC During mitosis, the 23 pairs of human chromosomes condense and are visible with a light microscope. A karyotype analysis usually involves blocking cells in mitosis. Autoradiograph , Definition of Autoradiograph. Feb 10, 2011 · This is a quick video on converting Wav files ...
0055]Sixty 30-day-old Wistar rats were randomly divided into 3 groups of 20. Each group was given a specific diet, the first ordinary chow (placebo), the second group chow of a specific composition S1 and the third group the same feed as Group 2 with additional components (S2); see Table 1. The diets contained identical quantities of proteins, carbohydrates, minerals and energy. At the age of 4 months, two of the four carotid arteries of half of each group of animals were occluded (2VO animals). The other half was subjected to a similar operation without occlusion of the arteries. At the age of 7 months the animals were sacrificed for further investigation. Inter alia, the receptor density in specific parts of the brain was determined with the aid of labelling using a radioactive marker substance. Three receptor types, the muscarinic 1, serotonergic 1A and the glutaminergic NMDA receptors were labelled in hippocampal slices by autoradiographic methods. The increased ratio of n-3 fatty acids in ...
The norepinephrine transporter (NET) radioligand (S,S)-[18F]FMeNER-D2 shows significant decreases in NET density in the human brain in Alzheimers disease: a post-mortem autoradiographic study ...
Gulyas B, Brockschnieder D, Nag S, Pavlova E, Kasa P, Beliczai Z, Legradi A, Gulya K, Thiele A, Dyrks T, Halldin C: The norepinephrine transporter (NET) radioligand (S,S)-[18F]FMeNER-D2 shows significant decreases in NET density in the human brain in Alzheimers disease: a post-mortem autoradiographic study., NEUROCHEMISTRY INTERNATIONAL 56: (6-7) pp. 789-798 ...
Yoo, J. H. and Borsodi, Anna and Tóth, Géza and Benyhe, Sándor and Gáspár, Róbert (2017) Characterization of [H-3] oxymorphone binding sites in mouse brain: Quantitative autoradiography in opioid receptor knockout mice. NEUROSCIENCE LETTERS, 643. pp. 16-21. ISSN 0304-3940 Samavati, Reza and Zádor, Ferenc and Szűcs, Edina and Tuka, Bernadett and Martos, Diána and Veres, Gábor and Gáspár, Róbert and Mándity, István and Vécsei, László and Benyhe, Sándor and Borsodi, Anna (2017) Kynurenic acid and its analogue can alter the opioid receptor G-protein signaling after acute treatment via NMDA receptor in rat cortex and striatum. JOURNAL OF THE NEUROLOGICAL SCIENCES, 376. pp. 63-70. ISSN 0022-510X Hajagos-Tóth, Judit and Bóta, Judit and Ducza, Eszter and Csányi, Adrienn and Tiszai, Zita and Borsodi, Anna and Samavati, Reza and Benyhe, Sándor and Gáspár, Róbert (2016) The effects of estrogen on the α2- adrenergic receptor subtypes in rat uterine function in late pregnancy in ...
The Southern blot is used to detect the presence of a particular piece of DNA in ... 6. Block with excess DNA. 7. Wash off unbound probe. 8. Autoradiograph ... - A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: ad542-Y2IwN
Winkongo (UE), June 5, GNA- A two week old baby was at the weekend found under a bridge in Winkongo, near Bolgatanga, the Upper East Regional Capital, being cuddled by a dog. The dog had not been found over night and after a rigorous search, it was found lying next to the baby, under a bridge quite close to where its owners farm is sited. Madam Rosemary Azure, Talensi-Nabdam District Director of Health, disclosed this in an intervie
A Florida man named Pattreon Stokes was arrested on multiple drug charges, and when he was searched, police found over $1,000 in drug money up his rectum...
Hello, , , Im looking for murine anti-TNF and anti IL-1 mAbs to use on cryostat cut , mouse lung tissue sections. I know that such Abs are available for FACS , analysis and ELISA work, however I have failed to find any specifically , for immunohistochemistry. First of all, does it really matter? Can I use , Abs generally used in ELISAs or FACS analysis in immuno-histo? and , secondly does anyone know where to get such Abs, that have been proven to , work on tissue sections. , , , I look forward to your comments and help, , , , Dr. A. Kadioglu , , ak13 at le.ac.uk ...
Mouse anti-BrdU antibody, clone Bu20a recognizes incorporated BrdU in a variety of cell types. It is suitable for use on tissue sections in double-labeling techniques.
Developments in scientific research follow improvements in apparatus- sources of 14C and autoradiography enabled Calvin to elucidate the pathways of carbon fixation. ...
Oncotarget | https://doi.org/10.18632/oncotarget.17541 Yonghua Bao, Qian Wang, Yongchen Guo, Zhiguo Chen, Kai Li, Yiqiong Yang, Huijuan Zhang, Huali Dong, Kui Shen, Wancai Yang
TY - JOUR. T1 - Autoradiographic analyses of Guinea pig airway tissues following inhalation exposure to C-labeled methyl isocyanate. AU - Kennedy, Amy L.. AU - Singh, Gurmukh. AU - Alarie, Yves. AU - Brown, William E.. PY - 1993/1/1. Y1 - 1993/1/1. N2 - Through the use of radioactively labeled methyl isocyanate (MIC), the deposition, penetration, and clearance of this highly reactive compound in the airway at the tissue and cellular levels have been directly examined. Guinea pigs were exposed to 14C-MIC vapors at concentrations ranging from 0.38 to 15.2 ppm for periods of 1-6 hr. Solubilization of tissues from these animals showed the airway tissues to have the highest level of radioactivity. In the nasal region, 14C deposition, as monitored by histoautoradiography, was limited to the epithelial layer, was related to dose, and was dependent on the specific epithelial cell type. The squamous epithelium was minimally labeled on the surface and the label did not penetrate the cell layer. However, ...
TY - JOUR. T1 - Cerebral glucose utilization is reduced in second test session. AU - Stapleton, June M.. AU - Morgan, Michael J.. AU - Liu, Xiang. AU - Yung, Babington C K. AU - Phillips, Robert L.. AU - Wong, Dean Foster. AU - Shaya, Elias K.. AU - Dannals, Robert F. AU - London, Edythe D.. PY - 1997/6. Y1 - 1997/6. N2 - Cerebral glucose utilization was higher during the first positron emission tomography (PET) session than during the second session, as assayed using the PET [18F]fluorodeoxyglucose method in male human volunteers. This difference was due largely to data from subjects with low trait anxiety, since subjects with high anxiety showed similar metabolism in both PET sessions. High anxiety subjects showed greater right/left ratios of cerebral metabolism than low-anxiety subjects, particularly during the second PET session. These findings suggest that the level of anxiety may be an important variable to consider in PET studies using multiple sessions.. AB - Cerebral glucose utilization ...
Because of their ability to modulate the Gamma Aminobutyric Acid (GABA) a receptor complex, the principal aim of the current work is to assess two essential heavy metals: Iron (Fe) and zinc (Zn) by atomic absorption in different brain areas of stressed rats. To do so, we investigated the effect of acute immobilization stress (single 1-hour session) on the distribution and the densities of GABAA receptors as well as the concentrations of Zn and Fe in several rat brain regions of the stressed rats. Animals were randomly assigned to either control or stress conditions and changes in specific binding of the GABAA receptor as labelled with T-Butylbicyclophosphonothionate (TBPS) (ligand useful for GABAA receptor) were assessed by in vitro quantitative autoradiography with the aid of a computer-assisted image analysis system whereas the assessment of Fe and Zn concentrations was done by atomic absorption spectrophotometry. Exposure to 1h immobilization stress led to a significant increase in [35S]-TBPS binding
The distribution of functionally active monoamine oxidase type A (MAO-A) was investigated by in vivo quantitative autoradiography using [14C]clorgyline in
Figure 1: (a) Microsatellite instability was originally assessed using gel electrophoresis and autoradiographic detection. In the left panel, additional bands (black arrows) in the tumor lane illustrate multiple contracted microsatellite alleles relative to the genomic control lane. In the right panel, an information (heterozygous) microsatellite is shown in the genomic control sample, and a significant loss of signal intensity for the smaller allele is observed in the tumor sample, characteristic of allelic imbalance/loss of heterozygosity (LOH). (b) Microsatellite loci are now commonly assessed using fluorescent PCR amplifications, capillary electrophoresis, and automated sequencing techniques. Laser scanners detect fluorescent PCR products and generate a chromatogram displaying microsatellite allele frequencies. Note in the tumor panel, one of the alleles has undergone contraction, depicting MSI in this tumor specimen. (c) Subcloning and direct sequencing of microsatellite amplifications can ...
Neurofunctional Imaging of Plasticity in Mouse Somatic Sensory Cortex during Development and in Maturity: The color-coded images show energy metabolism in sections cut tangentially through the right cortical hemisphere. The quantitative autoradiographic deoxyglucose method was employed for neurofunctional imaging. Outlines of the barrels in somatic sensory cortex are superimposed on the images (The nose is to the right; the midline is up). When six whiskers, that is whiskers B1-3 and D1-3, on the left side were stimulated, focal regions of increased metabolic nerve cell activity (red) were found co-localized with the corresponding barrels B1-3 and D1-3 (top image; row A is outside the plane of section; the arrow points at barrel E1). The distinct gap of low activity between the highly activated regions comprises barrels C1-3. When the follicles of whiskers C1-3 were removed on the day of birth, the barrels that would have represented these whisker did not develop and the adjacent barrels ...
We reviewed telomere reduction rates in human cells, tissues and organs (Table 1).7-11,13-24 These values were mostly within the range 20-60 bp per year. In an autoradiographic study in mice, cellular renewal times of hepatocytes, renal tubular and glomerular cells, and splenic tissue were found to be 460-480, 140-190 and 1-21 days, respectively,25 whereas very rapid renewal was found to occur in epithelial cells of the gastrointestinal mucosae, showing a turnover of within 7 days.12,25 Previous data we had obtained for annual telomere reduction in gastrointestinal mucosae9-11 showed values lower than 70 bp. Unlike mouse tissues and organs, renewal times of human tissues have been examined for only some types of lymphocytes.26 Data available for lymphocyte turnover times have shown considerably large differences between humans and mice.26,27 There are apparently no marked differences among telomere length reduction rates for the liver and renal cortex, and those we reported previously for ...
Zeng, B.Y., Pearce, R.K.B., MacKenzie, G.M. and Jenner, P. (2001) Chronic high dose L-dopa treatment does not alter the levels of dopamine D-1, D-2 or D-3 receptor in the striatum of normal monkeys An autoradiographic study. Journal of Neural Transmission, 108, 925-941.doi10.1007/s007020170013
The ability of normal and transplanted dog hearts to make, bind, store, and metabolize norepinephrine was studied. Transplanted hearts were used in order to assess the effects of adrenergic denervation. Normal hearts bound large quantities of administered C14-dopamine and synthesized considerable quantities of norepinephrine in both the atria and ventricles. Isolated perfused normal hearts steadily removed about 56% of infused dl-H3-norepinephrine; a binding mechanism was the major means of inactivation of the amine. Uptake of radioactive norepinephrine was greater in the ventricles than in the atria because they received more of the amine, and the turnover rate of the labeled amine was also highest in the ventricles. Subcellular fractionation of the bound amine demonstrated that it was localized in particles of microsomal size; radioautographic studies demonstrated the presence of H3-norepinephrine only in association with nerves. About 57% of the H3-norepinephrine released from normal hearts ...
In article ,3A7622A9.37BB48D9 at po-box.mcgill.ca,, Rachel ,rgeddy at po-box.mcgill.ca, wrote: , I am performing a radioactive in situ hybridization study and have come , to the point of developing my slides. Two tester slides have me quite , confused. Is the NTB-2 emulsion supposed to stay on the slide, , effectively covering the sections, or is it supposed to come off to , reveal the silver grains? All protocols I have seen neglect to mention , this detail, and I have one slide with a emulsion layer which wont come , off and one whose emulsion layer is coming off in pieces. The emulsion is very definitely supposed to stay on, it contains the silver grains. Think of it as like the film in a camera it is exactly equivalent. Your description of the symptoms suggest to me that the emulsion may be too thick, did you heat it before dipping the slides? Peter -- Peter Ashby Wellcome Trust Biocentre University of Dundee Dundee, Scotland Reverse the spam and remove to email me ...
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Pimonidazole immunofluorescence and64Cu-ATSM autoradiography. Pimonidazole immunofluorescence (A and C) and 64Cu-ATSM photoluminescence autoradiography (B and D
The effect of aerosol upon cloud properties is studied over the Baltic Sea region, which presents a distinct contrast of aerosol loading between the clean Fennoscandia and the polluted area of central-eastern Europe. Statistically significant positive values are found over the Baltic Sea and Fennoscandia, while negative values are found over central-eastern Europe, contradicting the theory of aerosol indirect effect on clouds ...
NEX306 [125I]Tyr0-Sauvagine has been shown to be active in receptor binding assays and has been used for receptor autoradiography. ...
Manufacturer: Vinci Technologies Model Number: HEP-P The Helium gas expansion porosimeter enables the determination of a samples (1" or 1.5" diameter) grain and pore volume via an isothermal helium expansion and the application of Boyles law and Charles law. Subsequently, porosity and grain density can be calculated. A data acquisition system performs calibration, data logging and parameter calculation. Optionally, a full diameter matrix cup can be provided for porosity studies on whole diameter core samples. Each sample matrix cup is interchangeable and is supplied with stainless steel calibration check plugs. Pore volume can be measured by placing the core sample in an optional Hassler type core holder, connected to the porosimeter.. FEATURES :. Core diameter: 1″ and 1.5″ , (4 optional ...
(1992) Karen Garner preparing a gorilla hair for analysis using PCR (polymerase chain reaction). This hair is from Peanuts, a silverback male mountain gorilla (named by Dian Fossey) who died May 1, 1989, at about age 29. The study is being done to assess the level of genetic diversity in gorilla populations, to clarify gorilla classification at the subspecies level, and to develop methods for pedigree determination. The bands (black) on the autoradiograms show the sequence of bases in a sample of DNA. At the San Diego Zoo in California. DNA Fingerprinting. MODEL RELEASED
I suggest listening to the admittedly rough rehearsal tracks first, so you can get a handle on what HEALTH HAZZARD are going for before you treat yourself to their crucial (if criminally short) two song demo. Once the edges were smoothed down and they decided that they deserved a proper studio session, these kids cranked out an under the radar two song banger well worth your time. Nearly three decades old, these are still the sounds that kick me in the ass - vocals barked with youthful insistence and razor sharp guitars cutting through the mix. The plodding pace of "Picture Show" suits the bleak lyrics perfectly...classic simple UK punk. Members went on to form REFUGEES, and the live bit from todays tape can be found over at Escape Is Terminal ...
The researchers studied 808 ICU admissions from July 1, 2015 to June 15, 2016 at Harbor-UCLA Medical Center and found that more than half the patients could have been cared for in less expensive and invasive settings.. Of the patients in the study, 23.4% were in need of close monitoring but not ICU-level care. Another 20.9% of the patients were critically ill but unlikely to recover because they had underlying illnesses or severity of acute illness. For another 8%, death was imminent or the same outcomes were expected in non-ICU care.. "Our study found over 50% of patients admitted to the ICU were categorized into groups suggesting that they were potentially either too well or too sick to benefit from ICU care or could have received equivalent care in non-ICU settings," said Dong W. Chang, MD, an LA BioMed researcher and the corresponding author for the study. "This research indicates that ICU care is inefficient because it is devoting substantial resources to patients who are less likely to ...
What makes one post more enjoyable to read or listen to over another? Here are some common elements Ive found over the years. | Bibliotecas Escolares
Bleicher , L.S., Barlow, C., Carter, T.A, Dearie, A.R., Eum, K., Franchini, B.R., Hofer, E.K., Lee, D.H., Lorrain, K.I., Pires, J.C., Rodriguez, J.J., Saxe, M.D., Stoveken, B.S., Wedel, P.C. (2010) Automated Image Capture and Analysis of BrdU Labeled Cells in Rat Brain Sections. Program No. 734.6. 2010 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience ...
Topics of the month Hello dear bloggers, I congratulate all the students who have qualified in gpat and other equivalent exams. here I present the topics of the month june 1)B.Nandini: a Autoradiography. b.Factors effecting pharmaceutical pack. 2)D.Narmadha: a.Driers in pharmaceutical application. b.Dissolution testing of lipid based formula.
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The methods of tissue preparation described above cannot be used when the procedures require that fixation-sensitive cellular proteins remain functional or when water-soluble, diffusible substances are to be localized in the tissue. In that case, the tissue must remain unfixed and immersion in sucrose cannot be used. The tissue has to be shock frozen at -70 ℃ and lower, and the sections must be kept frozen during sectioning. To fulfill this need the whole microtome is encased in a refrigerated cabinet. The instrument is then called a cryostat. Ten or 20 micrometer-thick sections are cut and picked up on chilled glass slides and quickly dried on a hotplate outside the cabinet. The subsequent procedures must consequently be carried out on the slide-mounted sections. This method is used for the autoradiography of diffusible radioactively-labeled tracers. For autoradiography, the sections are exposed to x-ray films or radiation-sensitive semiconductor chips that produce gray-scale images of the ...
Although it is now well documented (6, 8) that pial as well as intracerebral vessels are amply supplied with sympathetic adrenergic nerves which, as shown for pial arteries, fulfill ultra-structural...
en] Background: SV2A is the most studied isoform of the Synaptic Vesicle 2 proteins, which are involved in the synaptic vesicle trafficking. Interestingly, the SV2A has been identify as the binding site for the antiepileptic drug levetiracetam, showing a close relation between the epilepsy, the dysregulation of the SV2A levels and the response to antiepileptic medications. SV2A floxed-mice were developed using a cre-lox technique, leading to a strong decrease of SV2A expression in the CA3 field of the hippocampus. We aim here to validate this model using [18F]UCB-H, a novel PET imaging radiotracer with a nanomolar affinity for human SV2A ...
The localization of specific binding sites of four steroids--aldosterone, dexamethasone, 1-25-dihydroxycholecalciferol and estradiol--is described along the nephron. This localization has been determined by using an autoradiographic method on dry films, applied to intact microdissected tubular segments. Aldosterone binds specifically to nuclei of the distal and cortical collecting tubule. No specific labeling was observed in the cytoplasm. This localization corresponds to the known sites of action of aldosterone on sodium reabsorption. Specific nuclear binding of dexamethasone is present all along the tubule except the proximal tubule. In this latter part of the nephron, a specific cytoplasmic labeling is observed, in the absence of nuclear labeling. This cytoplasmic binding could correspond to physiological effects in this structure, via non-genomic mechanisms. 1-25(OH)2D3 nuclear binding is located mainly in the loop of Henle and medullary collecting tubule, which are the sites of synthesis of calcium
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Substance P (SP) and its tachykinin NK1 receptor (NK1R) function within key medullary nuclei to regulate cardiorespiratory and autonomic control. We examined the normative distribution of SP and NK1R in the serotonergic (5-Hydroxytryptamine, [5-HT]) network of the human infant medulla during postnatal development, to provide a baseline to facilitate future analysis of the SP/NK1R system and its interaction with 5-HT within pediatric brainstem disorders in early life. [125I] labelled Bolton Hunter SP (BH-SP) tissue receptor autoradiography (n = 15), single label immunohistochemistry (IHC) and double label immunofluorescence (IF) (n = 10) were used to characterize the normative distribution profile of SP and NK1R in the 5-HT network of the human infant medulla during postnatal development ...
The fourth track includes the neutron log, scaled in percent porosity. The bulk density log is scaled from 1.65 to 2.9 g/cc, so a sandstone with a grain density of 2.65 g/cc would have a porosity matching the neutron scale. The porosity measurements on core samples, labeled PORCORE, are scaled to match the neutron scale. Porosity increases to the left. Where available, total organic carbon (TOC) measurements, in weight percent, are also plotted in this track. ...
The present study examined the regional differences in dopamine transporter binding sites and NMDA receptor complex binding based on autoradiographic images obtained in postmortem sections of human normal brain tissues. in middle-aged control tissues, high and comparable levels of [H-3]CFT binding were observed in the caudate nucleus, putamen, and accumbens nucleus without significant alteration along the rostrocaudal axis and ventral and dorsal parts of these nuclei. in aging normal brain tissues, dopamine binding sites for [H-3]CFT were significantly reduced in the caudate nucleus, putamen, and accumbens nucleus. L-[H-3]Glutamate, [H-3]MK-801, and [H-3]glycine binding to the NMDA receptor complex was lower in aging brain tissues than in middle-aged controls. Significant correlation did occur between age and [H-3]CFT binding and between age and L-[H-3]glutamate, [H-3]MK-801, and [H-3]glycine binding sites. These results demonstrate that the basal ganglia have age-associated reductions in ...
We are interested in developing an airway explant culture system using sheep bronchi in which to establish respiratory viral infection and from which tissue can be used for functional, biochemical and immunohistochemical studies involving the endothelins (ETs). Freshly harvested sheep bronchial airway smooth muscle contains a homogeneous population of the ETA receptor. However, the potency of ET-1 and maximum contractile response of sheep bronchial explants to ET-1 increased with time in culture, despite these parameters remaining constant for carbachol in explants maintained for up to 48h. The possibility that this was caused by changes in ET receptor density was assessed using light microscopic quantitative autoradiography. In view of the increased responsiveness to ET-1 in cultured explants, it was surprising to demonstrate a significant decrease in total ET receptor (59±6% compared with the initial value, n = 4-5; P,0.01) and ETA receptor (51±2% compared with the initial value, n = 4-5, ...
Looking for online definition of cortical amygdaloid nucleus in the Medical Dictionary? cortical amygdaloid nucleus explanation free. What is cortical amygdaloid nucleus? Meaning of cortical amygdaloid nucleus medical term. What does cortical amygdaloid nucleus mean?
Four major, interdependent mechanisms are involved in the control of cerebral blood flow: metabolic coupling: neural control, involving both extrinsic and intrinsic neural pathways: Pco2 and autoregulation. Although this division may be somewhat artificial and these control mechanisms probably operate in concert, it is useful to consider each separately. Metabolic Control Local cerebral blood flow (CBF) is regionally heterogeneous. The varied pattern of CBF is neither random nor related to the anatomic organization of the cerebral vasculature or to known differences in the innervation patterns of the cerebral vessels. Neuronal activity is the principal energy-consuming process in the brain. Local cerebral blood flow adjusts to the level of energy generation; therefore, it is the activity in the neuronal circuits that is the major determinant of variations and regional patterns of cerebral blood flow. Normally there is exquisite coupling between the regional cerebral metabolic demand for oxygen ...

Characteristics of muscarinic cholinergic, γ-aminobutyric acid<sub>A</sub> and phencyclidine receptors in spontaneously...Characteristics of muscarinic cholinergic, γ-aminobutyric acid<sub>A</sub> and phencyclidine receptors in spontaneously...

... were examined using in vitro quantitative autoradiography. Computer analysis using autoradiographic technique revealed that the ... were examined using in vitro quantitative autoradiography. Computer analysis using autoradiographic technique revealed that the ... were examined using in vitro quantitative autoradiography. Computer analysis using autoradiographic technique revealed that the ... were examined using in vitro quantitative autoradiography. Computer analysis using autoradiographic technique revealed that the ...
more infohttps://pure.fujita-hu.ac.jp/en/publications/characteristics-of-muscarinic-cholinergic-%CE%B3-aminobutyric-acidsuba

Autoradiography Film | Fisher ScientificAutoradiography Film | Fisher Scientific

Recommended for direct autoradiography of weak and medium energy β-radioisotopes, such as 35S,33P, and 14C ... whether from chemiluminescent Western blot or DNA or RNA autoradiography. ...
more infohttps://www.fishersci.com/us/en/products/life-sciences/life-science-equipmentandinstruments/autoradiography-supplies/autoradiography-film.html

Detection of 3H-Leu by Autoradiography ?Detection of 3H-Leu by Autoradiography ?

... Samuel C. Blackman scb1 at midway.uchicago.edu Fri May 16 15:22:51 EST 1997 *Previous ... transfer to nitrocellulose or PVDF and then detect the labelled proteins by autoradiography. Ive heard that it is possible if ...
more infohttp://www.bio.net/bionet/mm/methods/1997-May/057559.html

Patent US5830629 - Autoradiography assemblage using transparent screen - Google PatentsPatent US5830629 - Autoradiography assemblage using transparent screen - Google Patents

An assemblage useful for autoradiography comprising, in order, a sample layer containing a radioactively labeled biological ... 1995 , entitled AUTORADIOGRAPHY ASSEMBLAGE USING TRANSPARENT SCREEN.. FIELD OF THE INVENTION. The present invention is directed ... This field is sometimes referred to as autoradiography.. BACKGROUND OF THE INVENTION. An autoradiograph is a recording of the ... Electronic imaging system for autoradiography. US6521329 *. 18 Jun 2001. 18 Feb 2003. Eastman Kodak Company. Radiographic ...
more infohttp://www.google.com.au/patents/US5830629

Imaging salt creep by autoradiography of36Cl−-tagged salt grains | SpringerLinkImaging salt creep by autoradiography of36Cl−-tagged salt grains | SpringerLink

... to produce a radioactive rim that was imaged by autoradiography. The tagged salt was compacted at pressures up to 138 MPa /20 ... to produce a radioactive rim that was imaged by autoradiography. The tagged salt was compacted at pressures up to 138 MPa /20 ...
more infohttps://link.springer.com/article/10.1007/BF02164970

Hormonal Receptor Plasticity in the Brain as Shown by In Vitro Quantitative Autoradiography | SpringerLinkHormonal Receptor Plasticity in the Brain as Shown by In Vitro Quantitative Autoradiography | SpringerLink

Quantitative autoradiography of serotonin receptors in the rat brain. Brain Res. 242: 197-204.PubMedCrossRefGoogle Scholar ... Serotonin receptor localization in rat brain by light microscopic autoradiography. Eur. J. Pharmacol. 62: 237-239.PubMed ... Receptor autoradiography with tritium-sensitive film: potential for computerized densitometry. Neuroscience Lett. 25: 101-105. ... Estradiol-concentrating neurons: Topography in the hypothalamus by dry-mount autoradiography. Science 162: 1001-1003.PubMed ...
more infohttps://link.springer.com/chapter/10.1007/978-1-4684-5131-3_34

Autoradiography - Current ProtocolsAutoradiography - Current Protocols

This overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial ... Advances in autoradiography: A new film brings quality improvements. In The Biotechnology Report pp. 147‐149. Campden ... Quantitative autoradiography is described as a means of calibrating experimental results. Importantly, specific guidelines are ... This overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial ...
more infohttp://www.currentprotocols.com/WileyCDA/CPUnit/refId-ps1011.html

TNO Repository search for: subject:AutoradiographyTNO Repository search for: subject:'Autoradiography'

Animal cell · Animal model · Gene rearrangement · Mouse · Nonhuman · Oncogene c myc · Animal · Autoradiography · Bone Marrow · ... Health · agar gel electrophoresis · autoradiography · blood sampling · cohort analysis · dna determination · genetic model · ... Nutrition · Radioisotope · Tin chloride · Animal experiment · Autoradiography · Blood and hemopoietic system · Electron ... Autoradiography · Bacterial Toxins · Carbon Radioisotopes · Cell Adhesion Molecules · Cells, Cultured · Cytotoxins · E-Selectin ...
more infohttps://repository.tudelft.nl/search/tno/?q=subject:%22Autoradiography%22

Forschungszentrum Jülich  -  Autoradiography - In vitro techniques: Autoradiography, Immunohistochemistry, Mass spectrometryForschungszentrum Jülich - Autoradiography - In vitro techniques: Autoradiography, Immunohistochemistry, Mass spectrometry

Quantitative Receptor Autoradiography. Using in-vitro autoradiography we analyze a broad range of different neuroreceptors in ... In vitro techniques: Autoradiography, Immunohistochemistry, Mass spectrometry. Many physiological mechanisms as well as ... 2: Definition of regions in autoradiography: via histological stainings (right) it is possible to quantify the receptor density ...
more infohttp://www.fz-juelich.de/inm/inm-2/EN/Research/Neuroimaging/Methods/Autoradiography/artikel.html

Micro autoradiographyMicro autoradiography

Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions suitable for Autoradiography ... Ilford emulsions for autoradiography. Add to Order. Add to Quote. More Information Ilford offers nuclear emulsions with either ... Micro autoradiography. Agar Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions ... Kodak NTB emulsion for autoradiography. Add to Order. Add to Quote. More Information Kodak manufactures nuclear emulsion type ...
more infohttps://www.agarscientific.com/lm/micro-autoradiography

Micro autoradiographyMicro autoradiography

Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions suitable for Autoradiography ... Ilford emulsions for autoradiography. Add to Quote. More Information Ilford offers nuclear emulsions with either 0.20µm (K) or ... Micro autoradiography. Agar Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions ... Kodak NTB emulsion for autoradiography. Add to Quote. More Information Kodak manufactures nuclear emulsion type NTB for use in ...
more infohttp://www.agarscientific.com/fr/lm/micro-autoradiography

Occupancy of central neurotransmitter receptors by risperidone, clozapine and haloperidol, measured ex vivo by quantitative...Occupancy of central neurotransmitter receptors by risperidone, clozapine and haloperidol, measured ex vivo by quantitative...

... measured ex vivo by quantitative autoradiography.. Schotte A1, Janssen PF, Megens AA, Leysen JE. ... were investigated precisely using receptor autoradiography in sections of the same rat brain except for histamine H1 receptors ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/7510574?dopt=Abstract

Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets | ScienceQuantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets | Science

Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets ... Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets ... Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets ... Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets ...
more infohttp://science.sciencemag.org/content/217/4565/1155

AIDS vaccine production: DNA autoradiography - Stock Image G210/0383 - Science Photo LibraryAIDS vaccine production: DNA autoradiography - Stock Image G210/0383 - Science Photo Library

Scientists studying a DNA sequencing autoradiogram of novel recombinant proteins cloned for the production of VaxSyn, an experimental AIDS vaccine. VaxSyn is based on a genetically- engineered, recombinant form of the HIV protein gp160, a precursor to one of HIVs surface proteins. VaxSyn contains no attenuated infectious parts of HIV & therefore carries no risk of transmitting AIDS. The vaccine is being developed by MicroGeneSys and is undergoing trials as a treatment of HIV-positive patients & as a potential vaccine against HIV infection. - Stock Image G210/0383
more infohttp://www.sciencephoto.com/media/210949/view/aids-vaccine-production-dna-autoradiography

Evaluation of Inhibitory Effect of Recreational Drugs on Dopaminergic Terminal Neuron by PET and Whole-Body AutoradiographyEvaluation of Inhibitory Effect of Recreational Drugs on Dopaminergic Terminal Neuron by PET and Whole-Body Autoradiography

... ... imaging and quantitative whole-body autoradiography (QWBAR) with [18F]FDOPA. The images were applied for the measurement of ...
more infohttps://www.hindawi.com/journals/bmri/2014/157923/abs/

Characterization of potential precursor populations in the mouse olfactory epithelium using immunocytochemistry and...Characterization of potential precursor populations in the mouse olfactory epithelium using immunocytochemistry and...

... of potential precursor populations in the mouse olfactory epithelium using immunocytochemistry and autoradiography. M Schwartz ... receptor cell regeneration stimulated by olfactory bulbectomy using a combination of immunocytochemistry and autoradiography. ... of potential precursor populations in the mouse olfactory epithelium using immunocytochemistry and autoradiography ... of potential precursor populations in the mouse olfactory epithelium using immunocytochemistry and autoradiography ...
more infohttp://www.jneurosci.org/content/11/11/3556

Autoradiography | Article about Autoradiography by The Free DictionaryAutoradiography | Article about Autoradiography by The Free Dictionary

A photographic emulsion is placed... Explanation of Autoradiography ... Find out information about Autoradiography. A photographic technique used to localize a radioactive substance within a solid ... Autoradiography. Also found in: Dictionary, Thesaurus, Medical, Wikipedia. Autoradiography. A photographic technique used to ... autoradiography. [¦ȯd·ō‚rād·ē′äg·rə·fē] (engineering) A technique for detecting radioactivity in a specimen by producing an ...
more infohttp://encyclopedia2.thefreedictionary.com/Autoradiography

Metal Transfer among Organs Following Short- and Long-Term Exposures Using Autoradiography: Cadmium Bioaccumulation by the...Metal Transfer among Organs Following Short- and Long-Term Exposures Using Autoradiography: Cadmium Bioaccumulation by the...

The anatomical distribution of the radionuclide was visualized using autoradiography at predefined time points. The gills did ... Metal Transfer among Organs Following Short- and Long-Term Exposures Using Autoradiography: Cadmium Bioaccumulation by the ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/28299929

Angiotensin converting enzyme in the human basal forebrain and midbrain visualized by in vitro autoradiography.Angiotensin converting enzyme in the human basal forebrain and midbrain visualized by in vitro autoradiography.

Autoradiography. Enzyme Inhibitors / metabolism*. Female. Frontal Lobe / cytology, enzymology*. Humans. Male. Mesencephalon / ... converting enzyme was mapped in the human basal forebrain and midbrain by using quantitative in vitro autoradiography employing ...
more infohttp://www.biomedsearch.com/nih/Angiotensin-converting-enzyme-in-human/2153714.html

Functional activity of substantia nigra grafts reinnervating the striatum: neurotransmitter metabolism and (14C)2-deoxy-D...Functional activity of substantia nigra grafts reinnervating the striatum: neurotransmitter metabolism and (14C)2-deoxy-D...

Measurements of glucose utilization by (14C)deoxyglucose autoradiography indicated equivalent metabolic rates for the grafted ... Measurements of glucose utilization by (14C)deoxyglucose autoradiography indicated equivalent metabolic rates for the grafted ... 59 BASIC BIOLOGICAL SCIENCES; BRAIN; PHYSIOLOGY; DOPA; METABOLISM; DOPAMINE; GRAFTS; BIOLOGICAL FUNCTIONS; AUTORADIOGRAPHY; ... 2-deoxy-D-glucose autoradiography. [Rats]}. author = {Schmidt, R H, Ingvar, M, Lindvall, O, Stenevi, U, and Bjoerklund, A}. ...
more infohttps://www.osti.gov/etdeweb/biblio/5390130

Autoradiography of Mast Cells in Experimental Skin Tumors of Mice Injected with Radioactive Sulfur (S35) | Cancer ResearchAutoradiography of Mast Cells in Experimental Skin Tumors of Mice Injected with Radioactive Sulfur (S35) | Cancer Research

Autoradiography of Mast Cells in Experimental Skin Tumors of Mice Injected with Radioactive Sulfur (S35). G. Asboe-Hansen ... Stripping-film autoradiography of connective tissue in experimental skin tumors in mice injected intraperitoneally with S35, ... Autoradiography of Mast Cells in Experimental Skin Tumors of Mice Injected with Radioactive Sulfur (S35) ... Autoradiography of Mast Cells in Experimental Skin Tumors of Mice Injected with Radioactive Sulfur (S35) ...
more infohttp://cancerres.aacrjournals.org/content/13/8/587

EN3HANCE™ Spray Surface Autoradiography Enhancer, 2oz Spray Can from PerkinElmer, Inc.  | SelectScienceEN3HANCE™ Spray Surface Autoradiography Enhancer, 2oz Spray Can from PerkinElmer, Inc. | SelectScience

Spray Surface Autoradiography Enhancer, 2oz Spray Can from PerkinElmer, Inc. on SelectScience ... EN3HANCE™ Spray Surface Autoradiography Enhancer, 2oz Spray Can. EN3HANCE™ Spray Surface Autoradiography Enhancer, 2oz Spray ... EN3HANCE™ Spray Surface Autoradiography Enhancer, 2oz Spray Can. Manufacturer PerkinElmer, Inc. Be the first to review this ...
more infohttp://www.selectscience.net/products/en3hance-spray-surface-autoradiography-enhancer,-2oz-spray-can/?prodID=81452&u=6CA65281-03F0-45B0-86DC-E0DFF0A61A65&techBID=41

Kodak X-omat Ar (xar) Autoradiography Film - Xar-5 Film (alternate Interleaved)Kodak X-omat Ar (xar) Autoradiography Film - Xar-5 Film (alternate Interleaved)

Autoradiography Film - XAR-5 Film (Alternate Interleaved) : General-purpose film for use with commonly used isotopes and chemi ... Home » Kodak X-omat Ar (xar) Autoradiography Film - Xar-5 Film (alternate Interleaved). ... Kodak X-omat Ar (xar) Autoradiography Film - Xar-5 Film (alternate Interleaved) Gift-wrapping is not available for this product ... This product may be an accessory for KODAK X-OMAT AR (XAR) Autoradiography Film, Carestream Health and may differ from the ...
more infohttp://www.egeneralmedical.com/vwr-ib1651454c.html

Increased Nicotinic Receptors in Brains from Smokers: Membrane Binding and Autoradiography Studies | Journal of Pharmacology...Increased Nicotinic Receptors in Brains from Smokers: Membrane Binding and Autoradiography Studies | Journal of Pharmacology...

Increased Nicotinic Receptors in Brains from Smokers: Membrane Binding and Autoradiography Studies. David C. Perry, Martha I. ... Increased Nicotinic Receptors in Brains from Smokers: Membrane Binding and Autoradiography Studies. David C. Perry, Martha I. ... Increased Nicotinic Receptors in Brains from Smokers: Membrane Binding and Autoradiography Studies. David C. Perry, Martha I. ... Increased Nicotinic Receptors in Brains from Smokers: Membrane Binding and Autoradiography Studies ...
more infohttp://jpet.aspetjournals.org/content/289/3/1545.long

Localization of Tritiated Digoxin in Dog Myocardium by Electron Microscopic Autoradiography | Circulation ResearchLocalization of Tritiated Digoxin in Dog Myocardium by Electron Microscopic Autoradiography | Circulation Research

Localization of Tritiated Digoxin in Dog Myocardium by Electron Microscopic Autoradiography. F. EUGENE TUBBS, LAMAR CREVASSE, ... Localization of Tritiated Digoxin in Dog Myocardium by Electron Microscopic Autoradiography. F. EUGENE TUBBS, LAMAR CREVASSE ... Localization of Tritiated Digoxin in Dog Myocardium by Electron Microscopic Autoradiography. F. EUGENE TUBBS, LAMAR CREVASSE ...
more infohttp://circres.ahajournals.org/content/14/3/236
  • Here, we accessed side effects of recreational drugs such as ketamine, cocaine, and methamphetamine on dopamine neurons in peripheral organs by using positron emission tomography (PET) imaging and quantitative whole-body autoradiography (QWBAR) with [ 18 F]FDOPA. (hindawi.com)
  • Manganese Imaging with Positron Emission Tomography,Autoradiography, and Magnetic ResonancebyGeoffrey John ToppingA THESIS SUBMITTED IN PARTIAL FULFILMENT OFTHE REQUIREMENTS FOR THE DEGREE OFDOCTOR OF PHILOSOPHYinThe Faculty of Graduate and Postdoctoral Studies(Physics)THE UNIVERSITY OF BRITISH COLUMBIA(Vancouver)February 2014? (ubc.ca)
  • Fig. 2: Definition of regions in autoradiography: via histological stainings (right) it is possible to quantify the receptor density in regions of interest (left. (fz-juelich.de)
  • We have studied which cell type is induced to divide during receptor cell regeneration stimulated by olfactory bulbectomy using a combination of immunocytochemistry and autoradiography. (jneurosci.org)
  • Autoradiography helps to determine the distribution and density of muscarinic receptor (MR) binding sites in the brain. (cas.cz)
  • After functional recordings, brain was removed and processed for autoradiography (brain stem sequential slices hybridized with 35 S-oligodeoxynucleotide probes for angiotensinogen [Aogen] and angiotensin II type 1 [AT 1A ] receptors). (ahajournals.org)
  • VEGFR, BrdU, lectin staining, and 125 I-VEGF 165 autoradiography on stroke brain tissue slices were performed to validate the in vivo findings. (ahajournals.org)
  • Measurements of glucose utilization by (14C)deoxyglucose autoradiography indicated equivalent metabolic rates for the grafted tissue and the intact substantia nigra. (osti.gov)
  • Stripping-film autoradiography of connective tissue in experimental skin tumors in mice injected intraperitoneally with S 35 , with sodium sulfate as carrier, showed that the majority of the mast cells take up sulfur. (aacrjournals.org)
  • Tissue distribution data were obtained by quantitative whole-body autoradiography. (ovid.com)
  • The two hapten molecules comprising 1,4,7-triazacyclononanetriacetic acid chelate moiety (NOTA) were labeled with (67)Ga or (68)Ga. The autoradiography was conducted by incubating the tissue samples with the bispecific antibody TF2, followed by washing and incubation with one of the radiolabeled hapten molecules. (diva-portal.org)
  • CCD (charged coupled device) and CMOS imaging technologies can be applied to thin tissue autoradiography as potential imaging alternatives to using conventional film. (surrey.ac.uk)
  • The Tissue Distribution of the Haematopoetically Active Agents, Cyanocobalamin (57b12) and Inorganic Cobalt (60co), in the Rat Studied by Autoradiography. (semanticscholar.org)
  • In situ hybridisation (ISH) combined with autoradiography is a standard method of measuring the amount of gene expression in histological sections, but the methods used to quantify gene expression in the resulting digital images vary greatly between studies and can potentially give conflicting results. (biomedcentral.com)
  • An increase was also observed in the migration of epithelial cells along the villus, as revealed by [3H]thymidine incorporation and autoradiography in rats fed 900 ppm Sn2+ for 4 weeks. (tudelft.nl)
  • 2006) Digital autoradiography using CCD and CMOS imaging technology In: 15th International Workshop on Room-Temperature Semiconductor X- and Gamma-Ray Detectors/ 2006 IEEE Nuclear Science Symposium, 2006-10-29 - 2006-11-04, San Diego, CA. (surrey.ac.uk)
  • The present study was performed to elucidate the mechanisms and morphological correlates underlying the crossed-withdrawal reflex by using quantitative 14 C-2-deoxyglucose (2-DG) autoradiography which can examine metabolic activities and spatial patterns simultaneously. (hindawi.com)
  • Agar Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions suitable for Autoradiography studies and the detection of radioactively labelled samples. (agarscientific.com)
  • The surfaces of crushed salt /NaCl/ grains were equilibrated with 36 Cl − to produce a radioactive rim that was imaged by autoradiography. (springer.com)
  • Autoradiography can be used to detect, and measure semiquantitatively, the radioactive materials in almost any object that can be placed in contact with film or photographic emulsion in some form. (thefreedictionary.com)
  • The photographic image of the distribution of radioactive substances in the observed object obtained through autoradiography is called an autoradiogram or radioautograph. (thefreedictionary.com)
  • Autoradiography can help establish the presence of radioactive elements in various ores, the distribution of natural radioactive elements in tissues of plant and animal organisms, and so on. (thefreedictionary.com)
  • Autoradiography of Water-Soluble Substances A. PRECIPITATION M E T H O D In order to prevent the leaching of radioactive water-soluble substances, 32 several methods have been used in autoradiography. (walkingreadinggroup.org)
  • She was a pioneer of the use of radioactive isotopes in biology and medicine, notably the techniques of radiocarbon dating and autoradiography. (wikipedia.org)
  • Rapidly remove haze and artifacts from X-ray film to correct overexposed blots, whether from chemiluminescent Western blot or DNA or RNA autoradiography. (fishersci.com)
  • An assemblage useful for autoradiography comprising, in order, a sample layer containing a radioactively labeled biological sample in contact with a phosphor layer, a film layer and a reflector layer. (google.com.au)
  • Advances in autoradiography: A new film brings quality improvements. (currentprotocols.com)
  • This product may be an accessory for KODAK X-OMAT AR (XAR) Autoradiography Film, Carestream Health and may differ from the image shown. (egeneralmedical.com)
  • KODAK X-OMAT AR (XAR) Autoradiography Film - XAR-5 Film (Alternate Interleaved) : General-purpose film for use with commonly used isotopes and chemiluminescence. (egeneralmedical.com)
  • The Global Autoradiography Film Market report by QY Market Research is an insightful analysis of the existing, future, and historic market scenario providing the reader an atomistic as well as a holistic view of the market. (talkdailynews.com)
  • The Autoradiography Film report provides the globe segmentation of the Autoradiography Film market on the basis of region, applications, end-users, and technology. (talkdailynews.com)
  • The global Autoradiography Film report is an essential reserve of data, primarily for the industry administrators. (talkdailynews.com)
  • On request for the worldwide Autoradiography Film market by anyone, they will be offered with a wide-ranging data about the particular industry encompassing all its aspects in the report. (talkdailynews.com)
  • Autoradiography is widely used in physics, technology, biology, and medicine-in all cases where isotopic indicators are used. (thefreedictionary.com)
  • This overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial distribution of radioisotope‐labeled substances within a membrane or specimen material. (currentprotocols.com)
  • Phosphor imaging is a type of digital autoradiography that maps the distribution of U and Th in rocks and minerals. (columbia.edu)
  • The anatomical distribution of the radionuclide was visualized using autoradiography at predefined time points. (nih.gov)
  • She spent the 1947-48 academic year in the United States learning about the recently discovered techniques of radiocarbon dating and autoradiography, which she introduced to Europe. (wikipedia.org)
  • A technic is described for high resolution intracellular autoradiography in the electron microscope. (rupress.org)