The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)
Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).
An analgesic and antipyretic that has been given by mouth and as ear drops. Antipyrine is often used in testing the effects of other drugs or diseases on drug-metabolizing enzymes in the liver. (From Martindale, The Extra Pharmacopoeia, 30th ed, p29)
Compounds that are used in medicine as sources of radiation for radiotherapy and for diagnostic purposes. They have numerous uses in research and industry. (Martindale, The Extra Pharmacopoeia, 30th ed, p1161)
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Cell surface receptors that bind signalling molecules released by neurons and convert these signals into intracellular changes influencing the behavior of cells. Neurotransmitter is used here in its most general sense, including not only messengers that act to regulate ion channels, but also those which act on second messenger systems and those which may act at a distance from their release sites. Included are receptors for neuromodulators, neuroregulators, neuromediators, and neurohumors, whether or not located at synapses.
The sum of the weight of all the atoms in a molecule.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
Radioactive substances added in minute amounts to the reacting elements or compounds in a chemical process and traced through the process by appropriate detection methods, e.g., Geiger counter. Compounds containing tracers are often said to be tagged or labeled. (Hawley's Condensed Chemical Dictionary, 12th ed)
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Detection and counting of scintillations produced in a fluorescent material by ionizing radiation.
Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes.
2-Deoxy-D-arabino-hexose. An antimetabolite of glucose with antiviral activity.
Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.
Unstable isotopes of fluorine that decay or disintegrate emitting radiation. F atoms with atomic weights 17, 18, and 20-22 are radioactive fluorine isotopes.
The rate dynamics in chemical or physical systems.
Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.
An imaging technique using compounds labelled with short-lived positron-emitting radionuclides (such as carbon-11, nitrogen-13, oxygen-15 and fluorine-18) to measure cell metabolism. It has been useful in study of soft tissues such as CANCER; CARDIOVASCULAR SYSTEM; and brain. SINGLE-PHOTON EMISSION-COMPUTED TOMOGRAPHY is closely related to positron emission tomography, but uses isotopes with longer half-lives and resolution is lower.
A benzodiazepine with pharmacologic actions similar to those of DIAZEPAM that can cause ANTEROGRADE AMNESIA. Some reports indicate that it is used as a date rape drug and suggest that it may precipitate violent behavior. The United States Government has banned the importation of this drug.
The measurement of the density of a material by measuring the amount of light or radiation passing through (or absorbed by) the material.
Unstable isotopes of copper that decay or disintegrate emitting radiation. Cu atoms with atomic weights 58-62, 64, and 66-68 are radioactive copper isotopes.
An anticonvulsant that is the active metabolite of TRIMETHADIONE.
A butyrophenone with general properties similar to those of HALOPERIDOL. It has been used in the treatment of aberrant sexual behavior. (From Martindale, The Extra Pharmacopoeia, 30th ed, p567)
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A subfamily of G-PROTEIN-COUPLED RECEPTORS that bind the neurotransmitter DOPAMINE and modulate its effects. D2-class receptor genes contain INTRONS, and the receptors inhibit ADENYLYL CYCLASES.
The study of the chemical and physical phenomena of radioactive substances.
A highly selective and specific beta antagonist that is used to characterize beta-adrenoceptors.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Cell surface proteins that bind somatostatin and trigger intracellular changes which influence the behavior of cells. Somatostatin is a hypothalamic hormone, a pancreatic hormone, and a central and peripheral neurotransmitter. Activated somatostatin receptors on pituitary cells inhibit the release of growth hormone; those on endocrine and gastrointestinal cells regulate the absorption and utilization of nutrients; and those on neurons mediate somatostatin's role as a neurotransmitter.
Elements of limited time intervals, contributing to particular results or situations.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Cell surface proteins that bind ANGIOTENSINS and trigger intracellular changes influencing the behavior of cells.
Cell-surface proteins that bind dopamine with high affinity and trigger intracellular changes influencing the behavior of cells.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An amphetamine analog that is rapidly taken up by the lungs and from there redistributed primarily to the brain and liver. It is used in brain radionuclide scanning with I-123.
Organic or inorganic compounds that contain the -N3 group.
A spiro butyrophenone analog similar to HALOPERIDOL and other related compounds. It has been recommended in the treatment of SCHIZOPHRENIA.
Isotopes that exhibit radioactivity and undergo radioactive decay. (From Grant & Hackh's Chemical Dictionary, 5th ed & McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The circulation of blood through the BLOOD VESSELS of the BRAIN.
A moderately lipophilic beta blocker (ADRENERGIC BETA-ANTAGONISTS). It is non-cardioselective and has intrinsic sympathomimetic actions, but little membrane-stabilizing activity. (From Martindale, The Extra Pharmocopoeia, 30th ed, p638)
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A subclass of IMIDES with the general structure of pyrrolidinedione. They are prepared by the distillation of ammonium succinate. They are sweet-tasting compounds that are used as chemical intermediates and plant growth stimulants.
A cardioselective beta-1-adrenergic antagonist with no partial agonist activity.
Striped GRAY MATTER and WHITE MATTER consisting of the NEOSTRIATUM and paleostriatum (GLOBUS PALLIDUS). It is located in front of and lateral to the THALAMUS in each cerebral hemisphere. The gray substance is made up of the CAUDATE NUCLEUS and the lentiform nucleus (the latter consisting of the GLOBUS PALLIDUS and PUTAMEN). The WHITE MATTER is the INTERNAL CAPSULE.
Small computers that lack the speed, memory capacity, and instructional capability of the full-size computer but usually retain its programmable flexibility. They are larger, faster, and more flexible, powerful, and expensive than microcomputers.
A neurotoxic isoxazole isolated from species of AMANITA. It is obtained by decarboxylation of IBOTENIC ACID. Muscimol is a potent agonist of GABA-A RECEPTORS and is used mainly as an experimental tool in animal and tissue studies.
A nitroimidazole that sensitizes normally radio-resistant hypoxic cells to radiation. It may also be directly cytotoxic to hypoxic cells and has been proposed as an antineoplastic.
The first artificially produced element and a radioactive fission product of URANIUM. Technetium has the atomic symbol Tc, atomic number 43, and atomic weight 98.91. All technetium isotopes are radioactive. Technetium 99m (m=metastable) which is the decay product of Molybdenum 99, has a half-life of about 6 hours and is used diagnostically as a radioactive imaging agent. Technetium 99 which is a decay product of technetium 99m, has a half-life of 210,000 years.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Analogs or derivatives of AMPHETAMINE. Many are sympathomimetics and central nervous system stimulators causing excitation, vasopressin, bronchodilation, and to varying degrees, anorexia, analepsis, nasal decongestion, and some smooth muscle relaxation.
Unstable isotopes of phosphorus that decay or disintegrate emitting radiation. P atoms with atomic weights 28-34 except 31 are radioactive phosphorus isotopes.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Stable strontium atoms that have the same atomic number as the element strontium, but differ in the atomic weight. Sr-84, 86, 87, and 88 are the stable strontium isotopes.
Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.
Any diagnostic evaluation using radioactive (unstable) isotopes. This diagnosis includes many nuclear medicine procedures as well as radioimmunoassay tests.
An organization of cells into an organ-like structure. Organoids can be generated in culture. They are also found in certain neoplasms.
Specific sites or molecular structures on cell membranes or in cells with which phencyclidine reacts or to which it binds to elicit the specific response of the cell to phencyclidine. Studies have demonstrated the presence of multiple receptor sites for PCP. These are the PCP/sigma site, which binds both PCP and psychotomimetic opiates but not certain antipsychotics, and the PCP site, which selectively binds PCP analogs.
A high-affinity muscarinic antagonist commonly used as a tool in animal and tissue studies.
The process by which a DNA molecule is duplicated.
Cell surface proteins that bind VASOACTIVE INTESTINAL PEPTIDE; (VIP); with high affinity and trigger intracellular changes which influence the behavior of cells.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Tomography using radioactive emissions from injected RADIONUCLIDES and computer ALGORITHMS to reconstruct an image.
Cell membrane proteins that bind opioids and trigger intracellular changes which influence the behavior of cells. The endogenous ligands for opioid receptors in mammals include three families of peptides, the enkephalins, endorphins, and dynorphins. The receptor classes include mu, delta, and kappa receptors. Sigma receptors bind several psychoactive substances, including certain opioids, but their endogenous ligands are not known.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Volume of biological fluid completely cleared of drug metabolites as measured in unit time. Elimination occurs as a result of metabolic processes in the kidney, liver, saliva, sweat, intestine, heart, brain, or other site.
Compounds with BENZENE fused to AZEPINES.
The compound is given by intravenous injection to do POSITRON-EMISSION TOMOGRAPHY for the assessment of cerebral and myocardial glucose metabolism in various physiological or pathological states including stroke and myocardial ischemia. It is also employed for the detection of malignant tumors including those of the brain, liver, and thyroid gland. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1162)
The thin layer of GRAY MATTER on the surface of the CEREBRAL HEMISPHERES that develops from the TELENCEPHALON and folds into gyri and sulchi. It reaches its highest development in humans and is responsible for intellectual faculties and higher mental functions.
A subclass of G-protein coupled SEROTONIN receptors that couple preferentially to GI-GO G-PROTEINS resulting in decreased intracellular CYCLIC AMP levels.
Cell-surface proteins that bind SEROTONIN and trigger intracellular changes which influence the behavior of cells. Several types of serotonin receptors have been recognized which differ in their pharmacology, molecular biology, and mode of action.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Neurotoxic proteins from the venom of the banded or Formosan krait (Bungarus multicinctus, an elapid snake). alpha-Bungarotoxin blocks nicotinic acetylcholine receptors and has been used to isolate and study them; beta- and gamma-bungarotoxins act presynaptically causing acetylcholine release and depletion. Both alpha and beta forms have been characterized, the alpha being similar to the large, long or Type II neurotoxins from other elapid venoms.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A genus of aquatic newts in the Salamandridae family. During breeding season many Triturus males have a dorsal crest which also serves as an accessory respiratory organ. One of the common Triturus species is Triturus cristatus (crested newt).
A method of chemical analysis based on the detection of characteristic radionuclides following a nuclear bombardment. It is also known as radioactivity analysis. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An essential branched-chain amino acid important for hemoglobin formation.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The directed transport of ORGANELLES and molecules along nerve cell AXONS. Transport can be anterograde (from the cell body) or retrograde (toward the cell body). (Alberts et al., Molecular Biology of the Cell, 3d ed, pG3)
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A class of compounds of the type R-M, where a C atom is joined directly to any other element except H, C, N, O, F, Cl, Br, I, or At. (Grant & Hackh's Chemical Dictionary, 5th ed)
A family of G-protein-coupled receptors that are specific for and mediate the effects of MELATONIN. Activation of melatonin receptors has been associated with decreased intracellular CYCLIC AMP and increased hydrolysis of PHOSPHOINOSITIDES.

Ambiguity of the thymidine index. (1/6999)

The observed thymidine indices of seven experimental tumor lines are compared as a function of duration of emulsion exposure. The effects of dose level of tritiated thymidine and background threshold are also evaluated. The results indicate that an arbitrary high background threshold discriminates against "lightly" labeled cells at short periods of exposure but that the chosen threshold becomes less critical with longer exposure. The observed thymidine index increases with increasing duration of emulsion exposure but appears to approach a plateau for all tumor systems. The "thymidine index curves" are significantly different for each tumor. There is an inverse relationship between the dose of tritiated thymidine and the duration of exposure required to recognize the same fraction of cells as labeled in a given tumor. Similar experimental conditions do not necessarily guarantee a valid basis for comparison of observed thymidine indices among tumors.  (+info)

Integrin subunit gene expression is regionally differentiated in adult brain. (2/6999)

Integrins are a diverse family of heterodimeric (alphabeta) adhesion receptors recently shown to be concentrated within synapses and involved in the consolidation of long-term potentiation. Whether neuronal types or anatomical systems in the adult rat brain are coded by integrin type was studied in the present experiments by mapping the relative densities of mRNAs for nine alpha and four beta subunits. Expression patterns were markedly different and in some regions complementary. General results and areas of notable labeling were as follows: alpha1-limited neuronal expression, neocortical layer V, hippocampal CA3; alpha3 and alpha5-diffuse neuronal and glial labeling, Purkinje cells, hippocampal stratum pyramidale, locus coeruleus (alpha3); alpha4- discrete limbic regions, olfactory cortical layer II, hippocampal CA2; alpha6-most prominently neuronal, neocortical subplate, endopiriform, subiculum; alpha7-discrete, all neocortical layers, hippocampal granule cells and CA3, cerebellar granule and Purkinje cells, all efferent cranial nerve nuclei; alpha8-discrete neuronal, deep cortex, hippocampal CA1, basolateral amygdala, striatum; alphaV-all cortical layers, striatum, Purkinje cells; beta4-dentate gyrus granule cells; beta5-broadly distributed, neocortex, medial amygdala, cerebellar granule and Purkinje cells, efferent cranial nerve nuclei; alpha2, beta2, and beta3-mRNAs not detected. These results establish that brain subfields express different balances of integrin subunits and thus different integrin receptors. Such variations will determine which matrix proteins are recognized by neurons and the types of intraneuronal signaling generated by matrix binding. They also could generate important differences in synaptic plasticity across brain systems.  (+info)

Activated macrophages and microglia induce dopaminergic sprouting in the injured striatum and express brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. (3/6999)

Nigrostriatal dopaminergic neurons undergo sprouting around the margins of a striatal wound. The mechanism of this periwound sprouting has been unclear. In this study, we have examined the role played by the macrophage and microglial response that follows striatal injury. Macrophages and activated microglia quickly accumulate after injury and reach their greatest numbers in the first week. Subsequently, the number of both cell types declines rapidly in the first month and thereafter more slowly. Macrophage numbers eventually cease to decline, and a sizable group of these cells remains at the wound site and forms a long-term, highly activated resident population. This population of macrophages expresses increasing amounts of glial cell line-derived neurotrophic factor mRNA with time. Brain-derived neurotrophic factor mRNA is also expressed in and around the wound site. Production of this factor is by both activated microglia and, to a lesser extent, macrophages. The production of these potent dopaminergic neurotrophic factors occurs in a similar spatial distribution to sprouting dopaminergic fibers. Moreover, dopamine transporter-positive dopaminergic neurites can be seen growing toward and embracing hemosiderin-filled wound macrophages. The dopaminergic sprouting that accompanies striatal injury thus appears to result from neurotrophic factor secretion by activated macrophages and microglia at the wound site.  (+info)

Development and cytodifferentiation of the rabbit pars intermedia. II. Neonatal to adult. (4/6999)

Material from pars intermedia obtained from rabbits ranging from the second week post-partum to the adult stage, and including specimens from pregnant animals, was studied. The rate of cell division became greatly reduced early in postnatal) development. The commonest type of cell (the pars intermedia-glandular cell) becomes increasingly PAS-positive during the early stages of development. Although by 35 days differentiation of all the ACT-type cells is complete, the pars intermedia-glandular cells take as long as 53 days to mature. The epithelioid border of the hypophysial cleft persists throughout life, commonly containing dark cells. A ciliary fringe frequently appears in neonates and persists in pregnancy. Possible functions of such cilia are discussed. Throughout development the fine structure of the vasculature was studied. Secretory granules resembling those within the cells were seen in and around the blood vessels, and the mode of endocrine secretion in the pars intermedia tissue is discussed. The pars intermedia-glandular cells of the pregnant rabbits appeared hyperactive. The functional significance of the mammalian pars intermedia is discussed.  (+info)

Onset of nucleolar and extranucleolar transcription and expression of fibrillarin in macaque embryos developing in vitro. (5/6999)

Specific aims were to characterize the onset of nucleolar and extranucleolar transcription and expression of the nucleolar protein fibrillarin during preimplantation development in vitro in macaque embryos using autoradiographic and immunocytochemical techniques. Autoradiography was performed on whole embryos cultured with [3H]uridine for assessment of nucleolar (rRNA) and extranucleolar (mRNA) transcription. Expression of fibrillarin was immunocytochemically assessed in whole embryos using a primary antibody against fibrillarin and a fluorescein isothiocyanate-conjugated secondary antibody. Extranucleolar incorporation of [3H]uridine was first detected in 2-cell embryos cultured 6-10 h with [3H]uridine. Culture with alpha-amanitin prevented incorporation of label in 2-cell embryos, and treatment with ribonuclease reduced the signal to background levels, indicating that [3H]uridine was incorporated into mRNA and not rRNA or DNA. Nucleolar incorporation of [3H]uridine was not evident in pronucleate-stage or 2- to 5-cell embryos, but it was detected in one 6-cell embryo and in all 8-cell to blastocyst-stage embryos. Fibrillarin was first expressed in some 6- to 7-cell embryos, but it was consistently expressed in all 8-cell embryos. Fibrillarin was localized to the perimeter of the nucleolar precursor bodies, forming a ring that completely encapsulated these structures. Fibrillarin was not expressed in 8- to 16-cell embryos cultured with alpha-amanitin, indicating that it is transcribed, rather than recruited, at the 8-cell stage. In conclusion, in in vitro-fertilized macaque embryos developing in vitro, extranucleolar synthesis of mRNA is initiated at the 2-cell stage while the onset of nucleolar transcription occurs at the 6- to 8-cell stage, coincident with expression of fibrillarin.  (+info)

The cerebral metabolic consequences of nitric oxide synthase deficiency: glucose utilization in endothelial and neuronal nitric oxide synthase null mice. (6/6999)

Nitric oxide has multiple physiologic roles in the CNS. Inhibiting nitric oxide synthesis might therefore alter functional activity within the brain. We used [14C]-2-deoxyglucose in vivo autoradiography to measure local CMRglc in "knockout" mice lacking the genes for either the endothelial (eNOS) or neuronal (nNOS) isoforms of nitric oxide synthase, and in the progenitor strains (SV129, C57B1/6). Glucose utilization levels did not significantly differ between nNOS and eNOS knockout mice and C57B1/6 mice in any of the 48 brain regions examined, but were relatively lower in some subcortical regions in SV129 mice.  (+info)

Age-related reductions in [3H]WIN 35,428 binding to the dopamine transporter in nigrostriatal and mesolimbic brain regions of the fischer 344 rat. (7/6999)

In the present study, we used the potent cocaine analog [3H]WIN 35, 428 to map and quantify binding to the dopamine transporter (DAT) within the dorsal striatum, nucleus accumbens, substantia nigra, and ventral tegmental area in young (6-month-old), middle-aged (12-month-old), and aged (18- and 24-month-old) Fischer 344 rats. Quantitative autoradiographic analysis of indirect [3H]WIN 35,428 saturation curves revealed two-site binding for all four brain regions in every age group. The percentage of binding to the high- or low-affinity sites did not differ with age or region and was approximately 50%. However, significant age-related decreases in the overall density (Bmax) of [3H]WIN 35,428-binding sites were observed in the striatum, nucleus accumbens, substantia nigra, and ventral tegmental area. The Bmax within all brain regions declined by more than 15% every 6 months, with the Bmax in the aged (24-month-old) group being approximately half that measured in the young adult (6-month-old) group. Competition experiments indicated that nomifensine also exhibited two-site binding to the DAT in Fischer 344 rats. No consistent age-related differences in binding affinities were noted with either [3H]WIN 35,428 or nomifensine. Taken together, these results support the hypothesis that functional DATs within the nigrostriatal and mesolimbic systems are down-regulated with age, without changing their affinity for ligands.  (+info)

Effect of diabetes and aminoguanidine therapy on renal advanced glycation end-product binding. (8/6999)

BACKGROUND: Advanced glycation end-products (AGEs) have been implicated in the pathogenesis of diabetic nephropathy, and aminoguanidine (AG) has been shown to decrease the accumulation of AGEs in the diabetic kidney. METHODS: This study investigates changes in AGE binding associated with diabetes in the rat kidney using in vitro and in vivo autoradiographic techniques. Male Sprague-Dawley rats were randomized into control and diabetic groups with and without AG treatment and were sacrificed after three weeks. Frozen kidney sections (20 microm) were incubated with [125I]-AGE-RNase or [125I]-AGE-BSA. To localize the AGE binding site, in vivo autoradiography was performed by injection of 15 microCi of [125I]-AGE-BSA into the abdominal aorta of the rat. RESULTS: Low-affinity binding sites specific for AGEs in the renal cortex (IC50 = 0.28 microm) were detected by in vitro autoradiography. There was a significant increase in [125I]-AGE binding in the diabetic kidney, which was prevented by AG treatment. Emulsion autoradiography revealed that binding was localized primarily to proximal tubules in the renal cortex. Renal AGE levels, as assessed by fluorescence or by radioimmunoassay, were increased after three weeks of diabetes. This increase was attenuated by AG therapy. CONCLUSIONS: AGE binding sites are present within the proximal tubules of the kidney and appear to be modulated by endogenous AGE levels. It remains to be determined if these binding sites represent receptors involved in clearance of AGEs or are linked to pathogenic pathways that lead to the development of diabetic nephropathy.  (+info)

After monolayer cultures of rat islets were exposed to [125I]insulin,[125I]glucagon, and [125I]tyrosinyl somatostatin, specific autoradiographic grains associated with each radioactively labeled ligand were found on B, A, and D cells.The density of labeling of the B, A, and D cells with each labeled ligand correlated well with the known actions of the three hormones on each of the islet cells. ...
Many physiological mechanisms as well as numerous neurological diseases involve the complex interaction of various receptors. Quantitative evaluation of the receptor distribution is essential for developing improved procedures in diagnosis and effective treatments of neurological diseases.. Quantitative Receptor Autoradiography. Using in-vitro autoradiography we analyze a broad range of different neuroreceptors in brain slices (10 -20 µm thick). After preincubation in buffer to remove endogenous ligand, sections are incubated with a radioactive substance, that binds specificly to the receptor being studied (radioligand). For the assessment of non-specific binding, selected slices are incubated with the radioligand in presence of an specific antagonist. After washing and a rapid rinse in ice-cold water the sections are dried and exposed against phosphor-imaging plates or conventional film slides in combination with tritium activity standards (see figure 1) for quantitative evaluation. Specific ...
An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance. Alternatively, the autoradiograph is also available as a digital image (digital autoradiography), due to the recent development of scintillation gas detectors or rare earth phosphorimaging systems. The film or emulsion is apposed to the labeled tissue section to obtain the autoradiograph (also called an autoradiogram). The auto- prefix indicates that the radioactive substance is within the sample, as distinguished from the case of historadiography or microradiography, in which the sample is X-rayed using an external source. Some autoradiographs can be examined microscopically for localization of silver grains (such as on the interiors or exteriors of cells or organelles) in which the process is termed micro-autoradiography. For example, micro-autoradiography was used to examine whether atrazine was being ...
TY - JOUR. T1 - Tritiated 2‐deoxy‐D‐glucose. T2 - A high‐resolution marker for autoradiographic localization of brain metabolism. AU - Hammer, Ronald P.. AU - Herkenham, Miles. PY - 1984/1/1. Y1 - 1984/1/1. N2 - The technique for autoradiographic localization of 2‐deoxy‐D‐glucose (2DG) uptake has become a useful method for observing alterations of functional brain activity resulting from experimental manipulation. Autoradiographic resolution is improved using tritiated ([3H]) rather than carbon‐14 ([14C])2DG, due to the lower energy and shorter path of tritium emissions. In addition, lower 2DG uptake by white matter relative to gray matter is exaggerated in the [3H]2DG autoradiographs due to the greater absorption of tritium emissions by lipids. Using [3H]2DG, it is possible to observe differential metabolic labeling in various individual nuclei or portions of nuclei that is unresolvable using [14C]2DG in the awake, normal animal. Heterogeneous patterns of 2DG uptake seen only ...
Various techniques for detection of blood platelets in tissue section were evaluated in an incremental air embolism model of ischemia in order to further investigate the accumulation of platelets in brains subjected to cell-damaging ischemia. The experimental animals were dogs anesthetized with alpha-chloralose. A new autoradiographic technique was devised that allows precise localization of 111Indium-labeled platelets in brain sections. The technique is described in detail along with some effects of the label on platelet function and behavior. The technique can be performed in conjunction with 14C-iodoantipyrine autoradiographic measurement of blood flow without causing mutual interference or other forms of interaction. This permits the simultaneous investigation of local blood flow and deposition of platelets. ...
Double-tracer autoradiographic study of protein synthesis and glucose consumption in rats with focal cerebral ischemia. - T Christensen, T Balchen, T Bruhn, N H Diemer
A potent, synthetic cannabinoid was radiolabeled and used to characterize and precisely localize cannabinoid receptors in slide- mounted sections of rat brain and pituitary. Assay conditions for 3H- CP55,940 binding in Tris-HCl buffer with 5% BSA were optimized, association and dissociation rate constants determined, and the equilibrium dissociation constant (Kd) calculated (21 nM by liquid scintillation counting, 5.2 nM by quantitative autoradiography). The results of competition studies, using several synthetic cannabinoids, add to prior data showing enantioselectivity of binding and correlation of in vitro potencies with potencies in biological assays of cannabinoid actions. Inhibition of binding by guanine nucleotides was selective and profound: Nonhydrolyzable analogs of GTP and GDP inhibited binding by greater than 90%, and GMP and the nonhydrolyzable ATP analog showed no inhibition. Autoradiography showed great heterogeneity of binding in patterns of labeling that closely conform to ...
We used the highly selective 5-HT(6) receptor radioligand [(125)I]SB-258585 (4-iodo-N-[4-methoxy-3-(4-methylpiperazin-1-yl)phenyl]benzene-sulfonamide) to perform autoradiographic binding studies on the rat brain. High levels of specific binding occurred in the corpus striatum, nucleus accumbens, Islands of Calleja and the olfactory tubercle. A high level of binding also appeared in the choroid plexus. Moderate levels occurred in several regions of the hippocampal formation and in certain regions of the cerebral cortex, thalamus, hypothalamus, and substantia nigra; and very low levels in the globus pallidus, cerebellum, other mesencephalic regions, and the rhombencephalon. Displacement of total binding with 10 microM unlabelled SB-214111 (4-bromo-N-[4-methoxy-3-(4-methylpiperazin-1-yl)phenyl]benzene-sulfonamide), another selective 5-HT(6) receptor antagonist, or 10 microM unlabelled methiothepin, reduced binding to barely discernible levels. Some animals received unilateral injections of 6
We have investigated 5-HT1A (serotonin1A) and 5-HT2A (serotonin2A) receptor mRNA abundance and binding site densities in various neocortical and hippocampal regions of schizophrenics and control subjects. Age, agonal state (brain pH), and post mortem interval were included where necessary as covariates in our analyses. In schizophrenics, 5-HT1A binding site densities, determined autoradiographically by [3H]8-hydroxy-2,3-(dipropylamino)-tetralin ([3H]8-OH-DPAT), were significantly increased (+23%) in the dorsolateral prefrontal cortex, with a similar trend in anterior cingulate gyrus. These increases were not accompanied by any change in 5-HT1A receptor mRNA. No differences between the groups in [3H]8-OH-DPAT binding or 5-HT1A receptor mRNA were seen in superior temporal gyrus, striate cortex, or hippocampus. 5-HT2A binding sites, determined by [3H]ketanserin, were decreased in the dorsolateral prefrontal cortex (-27%) and parahippocampal gyrus (-38%) of schizophrenics, with a similar trend in cingulate
Blanks, J C. and Bok, D, An autoradiographic analysis of postnatal cell proliferation in the normal and degenerative mouse retina. (1977). Subject Strain Bibliography 1977. 3447 ...
Mendelsohn, M L., Autoradiographic analysis of cell proliferation in spontaneous breast cancer of c3h mouse. Iii. The growth factor. (1961). Subject Strain Bibliography 1961. 685 ...
Unmodified oligodeoxynucleotides (ODNs) were synthesized and tested for their ability to cross external eukaryotic cell membranes and to enter the cytosol and nucleus in tissue cultures. The ODNs were labeled with high-specific-activity [3H]thymidine (| or = 100 Ci/mmol), or [ alpha-32P]ATP or [ gamma-32P]ATP (300-1000 Ci/mmol; 1 Ci = 37 GBq), and the label was either in the central portion of the molecule or at the 3 or 5 end. The cells employed were for the most part 3T6 murine fibroblasts, grown in monolayers, either semiconfluent or confluent, but some experiments were carried out with chicken embryo fibroblasts or human HeLa cells. Parallel wells in the same experiment were prepared for electron microscopy or for cell fractionation and radioactivity assays. Electron microscopic autoradiography indicated that ODNs cross the external cell membrane, traverse the cytosol, and begin to enter the cell nucleus within a few seconds to 5 min at 37 degrees C in Dulbeccos medium without added serum. After
CCD (charged coupled device) and CMOS imaging technologies can be applied to thin tissue autoradiography as potential imaging alternatives to using conventional film. In this work, we compare two particular devices: a CCD operating in slow scan mode and a CMOS-based active pixel sensor, operating at near video rates. Both imaging sensors have been operated at room temperature using direct irradiation with images produced from calibrated microscales and radiolabelled tissue samples. We also compare these digital image sensor technologies with the use of conventional film. We show comparative results obtained with (14)C calibrated microscales and (35)S radiolabelled tissue sections. We also present the first results of (3)H images produced under direct irradiation of a CCD sensor operating at room temperature. Compared to film, silicon-based imaging technologies exhibit enhanced sensitivity, dynamic range and linearity.. ...
Regional measurements of 131I-9.2.27 distribution in human melanoma tumors were obtained using quantitative autoradiography. Tumors were removed from patients 72-96 h after they had received an i.v. injection of 9.15 mCi (100 mg) of 131I-9.2.27. The autoradiographic images showed that the radioactivity reaching the tumor was heterogeneously distributed. Areas of relative high and low uptake were selected in each tumor. Regions of high activity contained from 51 to 1371 nCi/g, while areas with low uptake had radioactivity ranging from 12 to 487 nCi/g. The reliability of the autoradiographic measurements was demonstrated by the strong positive correlation with direct tissue sample counting (r = 0.994 P , 0.001). Since comparative immunocytochemistry showed a homogeneous and diffuse staining of target antigen on viable tumor cells, variability of monoclonal antibody uptake within individual tumors was not primarily due to heterogeneity of antigen expression in these cases. However, antigen levels ...
The presence and abundance of 5-HT1A and 5-HT2A receptor mRNAs in post mortem human hippocampus was investigated using a novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) technique using cyclophilin mRNA as an internal standard. 5-HT1A and 5-HT2A receptor mRNAs were each co-amplified with varying dilutions of cyclophilin primers, and their abundance expressed as a ratio of cyclophilin mRNA. Using this technique in combination with quantitative autoradiography we have investigated the effect of aging on hippocampal 5-HT1A and 5-HT2A receptor mRNA abundance and binding site densities. There was a significant negative correlation between hippocampal 5-HT1A receptor binding site densities and age and a similar trend for 5-HT1A receptor mRNA abundance. Neither 5-HT2A receptor binding site densities nor mRNA abundance were affected by age. Both 5-HT1A and 5-HT2A receptor binding site densities in individual subjects correlated significantly with abundance of their encoding mRNA. This
Read Quantitative autoradiographic mapping of 5‐HT 3 receptors in the rat CNS using ( 125 I)iodo‐zacopride and ( 3 H)zacopride as radioligands, Synapse on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Distribution of 2-chloro-2 -deoxyadenosine, 2-chloro-2 -arabino-fluoro-2 -deoxyadenosine, Fludarabine and Cytarabine in mice: a whole-body autoradiography study ...
Ling, E.A. (1974). Ultrastructure and radioautographic studies of the thyroid gland. Annals of the Academy of Medicine Singapore 3 (3) : 207-211. [email protected] Repository ...
A technique has been developed that allows repeated autoradiographs to be made of the isotope distribution in the chromosomes of a single cell. A series of 10 separate autoradiographs were made of a Chinese hamster diploid male metaphase cell which had been labeled with tritiated thymidine during the first 15 minutes of its DNA synthesis period in the previous interphase. Each autoradiograph had low grain densities above the chromosomes so that quantitation was feasible. The separate autoradiographs were photographically combined into a single composite in which grain images were converted to lines oriented at right angles to the chromosome axis. The line densities were then measured with a recording microdensitometer to yield graphs reflecting the isotope distribution along each chromosome. The area under each graph was directly proportional to the total number of grains counted above the corresponding chromosome in the 10 separate autoradiographs. The distribution of isotope along the ...
Morphological analysis of transplantable pancreatic acinar carcinoma of rat has revealed a continuum of cells from those which totally lack mature secretory granules to cells with abundant well-formed secretory granules. This tumor is easily dissociable into single cells which incorporate [3H]thymidine and [3H]leucine into DNA and protein at a linear rate for 3 to 4 hr. The number of cells synthesizing DNA, as assessed by light microscope autoradiography, was about 17%. Dissociated neoplastic pancreatic acinar cells are classified into four cell types (types I to IV) on the basis of nuclear morphology, degree of polarization, and the extent of specialization of cytoplasmic organelles. Type IV cells possess all the characteristics of mature pancreatic acinar cells, such as abundant rough endoplasmic reticulum, prominent Golgi apparatus, and numerous secretory granules. Cell types III, II, and I are progressively less differentiated. An analysis of the cytological features of 285 dissociated tumor ...
Agar Scientific is pleased to be able to still offer a range of Kodak and Ilford films and emulsions suitable for Autoradiography studies and the detection of radioactively labelled samples.
The distribution of infused tritiated norepinephrine (NE-3H) in small mesenteric arteries and intestinal arterioles in rats was investigated with electron microscopic radioautography. Silver grains, indicating the presence of the tritium label on the sections, were found lying mainly over axon bundles, but some were present over collagen and smooth muscle cells. Axons with the highest concentrations of silver grains had been sectioned at points where they were naked of Schwann cell sheath, were dilated into varicosities, and contained small granular vesicles. This finding was taken as confirmatory circumstantial evidence that the small granular vesicles were the sites of uptake and storage of NE. The short interval between the start of infusion and the fixation of the tissue appeared to rule out any process other than a direct uptake of NE by the peripheral axons. If axonal sites of uptake of NE-3H correspond to sites of release of NE, then the evidence suggests that such sites of release are ...
The Center for Quantitative Cancer Imaging has as part of its basic science imaging infrastructure a Fuji BAS-5000 Phosphor imager. This device offers extremely high resolution (25 μm) and image quality. It uses Fujifilms unique confocal laser and light-collecting optics. The BAS-5000 is amenable to fine-structure studies of a wide variety of tissue samples. With a dynamic range up to five orders of magnitude and a pixel size as small as 25 μm, the system allows for very high-resolution quantitative autoradiography studies. The system can provide rapid scan times. A 20 x 25 cm imaging plate can be scanned at 50 μm in as little as five minutes. The system includes software for region of interest (ROI) analysis and quantitative assessment of tracer accumulation. The system can image all PET isotopes as well as C-14 and tritium. The system also has the capabilities to perform various optical-based assays, including 2D electrophoresis and thin layer chromatography.. ...
Conceptual computer illustration of the DNA double helix together with a graphic representation of an autoradiograph display. The pattern of the DNA autoradiograph bands is unique to each individual, but some bands are shared by related people, such as a parent & child. DNA fingerprints can be used to prove conclusively whether people are related. - Stock Image C010/5265
The PMGKAY was announced in 2020 for three months till July and later extended till November to combat the economic impact of COVID-19 on the poor
The distribution of cells expressing mu-receptor mRNA and mu-receptor binding sites were compared in brain and spinal cord tissue sections using a combination of in situ hybridization and receptor autoradiographic techniques. mu-Receptor mRNA was visualized with a 35S-labeled cRNA probe directed to transmembrane III-VI of the rat mu-receptor, while mu-receptor binding sites were labeled with the mu-selective ligand [3H]DAMGO ...
TY - JOUR. T1 - Prediction of histopathological outcome using averaged multimodal information in rat. AU - Zhao, Weizhao. AU - Ginsberg, Myron D.. AU - Belayev, Ludmila. AU - Truettner, Jessie. AU - Schmidt-Kastner, Rainald. PY - 1998/12/1. Y1 - 1998/12/1. N2 - Commonly used autoradiographic image analysis for stroke research has been restricted to the assessment of local cerebral blood flow (LCBF), glucose utilization (LCMRglc) or messenger RNA (mRNA) (by in situ hybridization) in individual brains, with repeated measurements from the same animal. Histopathological analysis of perfusion-fixed paraffin-embedded brain material has long been regarded as the `gold standard for the quantitative assessment of tissue injury in experimental models of cerebral ischemia. It is desirable to reveal the interrelationship between signals measured at the time of ischemia and the final distribution of cell damage. In this study, averaged images for each modality were derived from individual rats and ...
The IVISR provides optical, CT, ultrasound, high-resolution planar gamma imaging, and PET of mice and rats, as well as high-resolution digital autoradiography and fluorescent imaging of thin whole body rodent sections.
Biospace Lab was founded in 1989 by G. Charpak to industrialize the world first digital autoradiography system and distribute it globally. He was awarded by
The autoradiographic analysis of neurotransmitter receptor distribution is a powerful technique that provides extensive information on the localization of neurotransmitter systems. Computer methodologies are described for the analysis of autoradiographic material which include quench correction, 3-dimensional display, and quantification based on anatomical boundaries determined from the tissue sections. These methodologies are applied to the problem of the distribution of glycine receptors measured by 3H-strychnine binding in the mouse CNS. The most distinctive feature of this distribution is its marked caudorostral gradient. The highest densities of binding sites within this gradient were seen in somatic motor and sensory areas; high densities of binding were seen in branchial efferent and special sensory areas. Moderate levels were seen in nuclei related to visceral function. Densities within the reticular formation paralleled the overall gradient with high to moderate levels of binding. The ...
Figure 1 Antiphospho-Pak1 antibody specifically recognizes activated Pak1. (A) Activation loop sequence of the phosphorylated peptide used for production of polyclonal antiphospho-Pak1 antisera. (B) Immunoblots (lanes 1-4) and radiolabeled kinase reaction products (lanes 5 and 6) of baculovirus-produced Pak1. Purified KD or CA Pak1 was incubated in protein kinase buffer in the presence of ATP, then immunoblotted using anti-Pak1 (lanes 1 and 2) and antiphospho-Pak antisera (lanes 3 and 4) autoradiographed to detect antibody-bound proteins. Although both forms of the protein were detected with anti-Pak1, only activated Pak1 was detected using the phospho-specific antibody, despite the large amount of recombinant protein used in this assay. Lanes 5 and 6 show autoradiographed PAGE gel of reaction products from a kinase assay performed in the presence of [γ-32P]ATP. Molecular mass in kilodaltons is shown to the right of the blot. (C) Comparison between immunoblot and radiolabeled kinase reaction ...
The first concerns cellular mechanisms of learning and memory. There is considerable evidence that a crucial step in memory formation is the synthesis of new proteins in the hippocampus. We are using autoradiographic techniques to focus on proteins that are made in dendrites of hippocampal neurons, near synapses. Very few proteins are made there, but we have found out that electrical stimulation of the axons that make contact with these dendrites greatly stimulates their synthesis of proteins. This only happens when the stimulus is combined with another neurotransmitter, acetylcholine. There is reason to believe this phenomenon is important in memory formation and we are studying its mechanisms and significance. The second area concerns the regulation of synaptic transmission by glycolysis. We have found that synaptic transmission in the hippocampus will only occur if glycolysis is active, even if adequate energy is supplied in other ways. This is very unexpected. There appears to be a ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Ive had a decades-long fascination with herbs and natural healing and found over the years that many such treatments can be quite successful. Im...
The Ghana Socioeconomic Panel Survey 2009-2010 collected baseline information for a panel of 5,009 households (18,889 household members) across all regions of Ghana. Two questionnaires collected information about household demographics, housing characteristics, household and agricultural assets, agricultural production, household enterprises, health and fertility, and household consumption and expenditure. Cognitive tests were conducted for children in the household, and psychological tests were conducted for the household head, spouse, and a randomly chosen household member.. A community questionnaire was also used to collect information about community institutions, political organizations, development programs, and infrastructure. Geographic (GPS) information was also recorded for all households and community facilities.. ...
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We investigated the effect of a novel quinazoline derivative (KB-5666), a lipid peroxidation inhibitor, on ischemic neuronal damage using Mongolian gerbils. The animals were sacrificed 7 or 30 days after 5 min of forebrain ischemia induced by bilateral common carotid artery occlusion. Morphologic changes, a microtubule-associated protein 2 (MAP2) immunohistochemical study and quantitative autoradiographic study using [3H]phorbol 12, 13-dibutyrate ([3H]PDBu) were evaluated in the hippocampus after ischemia. KB-5666 (3-50 mg/kg, i.v.) showed protective effects against neuronal death of the CA1 subfield 5 min before ischemia, immediately or 1 hr after ischemia, but not 4 hr after ischemia. KB-5666 (i.p.) also showed protective effects in a dose-dependent manner immediately after ischemia. Furthermore, KB-5666 dose-dependently prevented a marked decrease in microtubule-associated protein 2 immunoreactivity in the dendritic fields of the CA1 pyramidal cells after ischemia. The [3H]PDBu binding ...
The intracellular transport of venom proteins has been studied in active and resting venom glands of the snake Vipera palaestinae by electron microscope radioautography after an intra-arterial injection of [3H]leucine. In the active gland, most of the label is initially (10 min) found over the RER. By 30 min, the relative grain density of the Golgi complex reaches its maximum, with concomitant increase in the labeling of the condensing vacuoles. Later on, a steep increase in radioactivity of the secretory granules is observed. At 3 h, these granules, which comprise about 2% of the cell volume, contain 22% of the total grains. At the following hour, their labeling declines and at the same time the radioactivity of the secreted venom is increased. It is concluded that, in the active cell, venom proteins are transported via the Golgi apparatus into membrane-bounded granules which are the immediate source of the secreted venom. An alternative pathway, which involves the RER cisternae as a storage ...
Grain counting by eye is a tedious and time-consuming technique but one with great potential in cell kinetics and for the study of DNA excision repair activity (unscheduled DNA synthesis or UDS). We have been investigating the levels of UDS in human skin sections exposed in situ to ultraviolet radiation using a short-term incubation in tritiated thymidine and autoradiography and the decline in UDS levels with time (repair kinetics). We have adapted an automated image analysis system automatically to assess the number of grains over epidermal cell nuclei in autoradiographs of sections of epidermis. An excellent correlation was observed between visual counting and machine measurement of the area (in pixels) occupied by silver grains. The levels of UDS declined with time as lesions are progressively repaired. The half time (+/- standard deviation) for the reduction in UDS is 7.25 +/- 0.18 h. The grain counts can be significantly increased by increasing the autoradiographic exposure, by increasing ...
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The publisher regrets that a mistake was published in this article. On page 747, the word antagonist was incorrectly published instead of agonist. The whole sentence should correctly read: Importantly, also in response to an adenosine A1 agonist, old rats had less sleep compared with young animals. Our sincere apologies are offered to the authors, editor, and readers.. The original article of this erratum was published in J Neurosci Res 2004; 78: 742-748 ...
Charles River designs and conducts GLP-compliant quantitative whole-body autoradiography (QWBA) programs integrated with our preclinical drug metabolism services.
An assemblage useful for autoradiography comprising, in order, a sample layer containing a radioactively labeled biological sample in contact with a phosphor layer, a film layer and a reflector layer. The phosphor layer is preferably a gadolinium oxysulfide containing layer and the film is preferably a photographic film containing a tabular grain emulsion.
TY - JOUR. T1 - Autoradiography of monoamine uptake in Molluscan ganglia. AU - Elekes, K.. PY - 1978/1/1. Y1 - 1978/1/1. UR - UR - M3 - Article. AN - SCOPUS:0018084061. VL - 10. SP - S268. JO - Neuroscience Letters. JF - Neuroscience Letters. SN - 0304-3940. IS - suppl. 1. ER - ...
Read independent reviews on EN3HANCE™ Spray Surface Autoradiography Enhancer, 2oz Spray Can from PerkinElmer, Inc. on SelectScience
DNA. Computer artwork of a DNA (deoxyribonucleic acid) double helix seen against autoradiograms of genetic sequences (upper right). The spiralling strands of the DNA helix (blue) are composed of complex chemical groups called nucleotides, which consist of a sugar phosphate and a base group. Complementary pairing between the base groups of nucleotides on opposite strands holds the helix together. The sequence of the 4 base groups (adenine, cytosine, guanine and thymine; coloured rods) along the DNA helix is unique for every individual and is known as the genetic code. The autoradiograms depict DNA base sequences, each band (dark blue) representing an individual base. - Stock Image G110/0642
The effect of aerosol upon cloud properties is studied over the Baltic Sea region, which presents a distinct contrast of aerosol loading between the clean Fennoscandia and the polluted area of central-eastern Europe. Statistically significant positive values are found over the Baltic Sea and Fennoscandia, while negative values are found over central-eastern Europe, contradicting the theory of aerosol indirect effect on clouds ...
A variety of methods have been used in published studies for image segmentation (manual outlining, thresholding, magic wand, use of templates, etc.) to determine what is part of the structure or region of interest and what is not. Similarly, once grey levels are measured, a variety of methods have been employed to convert them into optical densities or units of radioactivity. Based on the above results, the line method was the best way to select the structure or region of interest as it was not subject to floor-effects, had a low coefficient of variation, and low within-sample variability. This method is similar to, and a modification of the outline method, which requires that the actual boundaries of the structure be determined, whereas the line method sampled only from the interior of the structure. Since the DG is a long narrow structure, this was best done with a line down the centre. Larger structures can follow the same principle by outlining the structure but staying well inside the ...
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There are 13 fifteen-letter words containing A, D, G, U and Y: AUTORADIOGRAPHY DAGUERREOTYPERS DAGUERREOTYPIES ... PSEUDOPREGNANCY RADIOAUTOGRAPHY UNDERSTANDINGLY. Every word on this site can be used while playing scrabble. Create other lists, beginning with or ending with letters of your choice.
Hello, dears! Im back with another random photos post and BOY did I need to make one! I found over a hundred miscellaneous photos I wanted to use today. :O Heh heh, but dont worry, I cut it down to about half that amount with much labor. :P Sit back, relax, and start scrolling! New…
Hello, dears! Im back with another random photos post and BOY did I need to make one! I found over a hundred miscellaneous photos I wanted to use today. :O Heh heh, but dont worry, I cut it down to about half that amount with much labor. :P Sit back, relax, and start scrolling! New…
A Duke University study that found over 40 percent of our actions aren’t actually decisions, but habits. Heres how to build good ones. 
WhatsApp Links is very common found over the internet, maximum individuals like to enter various types of Whatsapp Groups chat invitations, so my
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Durie, B.; Salmon, S. (1975-12-12). "High speed scintillation autoradiography". Science. 190 (4219): 1093-1095. doi:10.1126/ ...
Autoradiography was then used by the Finsen Institute to investigate the effects of the radiocontrast agent thorotrast. Levi ... She developed the new technique of autoradiography while working for the United States Atomic Energy Commission at the ... Levi, Hilde; Boyd, George A. (20 January 1950). "Carbon 14 Beta Track Autoradiography". Science. 111 (2873): 58-59. Bibcode: ... notably the techniques of radiocarbon dating and autoradiography. In later life she became a scientific historian, and ...
... in which the process is termed micro-autoradiography. For example, micro-autoradiography was used to examine whether atrazine ... For in vitro autoradiography, radioligand was directly applying on frozen tissue sections without administration to the subject ... The rate of DNA replication in a mouse cell growing in vitro was measured by autoradiography as 33 nucleotides per second. The ... Applications for autoradiography are broad, ranging from biomedical to environmental sciences to industry. The use of ...
"Photographic image intensification by autoradiography". Applied Optics. 15 (11): 2860-2865. Bibcode:1976ApOpt..15.2860A. doi: ...
They used tritiated thymidine autoradiography to label the cells. Tritiated thymidine is incorporated into the DNA of dividing ... since no phenotypic markers were available that could be used in conjunction with thymidine autoradiography. The absence of ...
Huberman, J. A.; Riggs, A. D. (March 1, 1966). "Autoradiography of chromosomal DNA fibers from Chinese hamster cells". ...
Ullberg S, Ewaldsson B (February 1964). "Distribution of radio-iodine studied by whole-body autoradiography". Acta Radiologica ...
He pioneered research on autoradiography to follow translocation in plants. That work resulted in more than one hundred ...
Other label-dependent detection methods includes chemiluminescence, colorimetric or autoradiography. Label-dependent assays are ...
To detect any protein-DNA interactions, autoradiography is commonly used. "Southwestern blot mapping" is a time-efficient way ...
CI-994 autoradiography". Epigenetics. 8 (7): 756-764. doi:10.4161/epi.25202. ISSN 1559-2308. PMC 3781195. PMID 23803584. ...
Thus the surviving probe-mRNA complement is simply detected by autoradiography. Nuclease protection assays are used to map ...
Labeled with tritium WAY-100635 may also be used in autoradiography. WAY-100635 has higher 5-HT1A affinity than 8-OH-DPAT. WAY- ... WAY-100635 for 5-HT1A receptor autoradiography in human brain: a comparison with [3H]8-OH-DPAT and demonstration of increased ...
Labeled with tritium, WAY-100,635 may also be used in autoradiography. 2C-B 2C-E 2C-T-2 4C-T-2 5-CT 5-MT 5-MeO-DiPT 5-MeO-DMT 5 ... Burnet PW, Eastwood SL, Harrison PJ (June 1997). "[3H]WAY-100635 for 5-HT1A receptor autoradiography in human brain: a ...
He was the first to map the distribution of receptors in the lung using radioligand autoradiography. His group investigated the ... Localization of ß-adrenoceptors in mammalian lung by light microscopic autoradiography. Nature 1982; 299:444-447. PMID 6289123 ...
"Autoradiography with Tritiated Methotrexate and the Cellular Distribution of Folate Reductase". Science. 151 (3717): 1528-1530 ...
This is followed by autoradiography to detect the position of the label. The plaque hybridization procedure has some advantages ...
Cairns J (March 1963). "The bacterial chromosome and its manner of replication as seen by autoradiography". Journal of ...
Cairns, J. (1963). "The bacterial chromosome and its manner of replication as seen by autoradiography". J. Mol. Biol. 6 (3): ...
Pechblende (Prologue) employs analog photographic methods, including autoradiography, combining them with archival material. ...
As professor of neurobiology at Cornell University, she developed quantitative electron microscopic autoradiography as a means ... She developed quantitative electron microscopic autoradiography, and demonstrated it as a sensitive means to study the ... Salpeter, Miriam M. (1964). "Autoradiography with the electron microscope: a procedure for improving resolution, sensitivity, ... "Quantitation of junctional and extrajunctional acetylcholine receptors by electron microscope autoradiography after (125)I-α- ...
Jenkins EC (January 1972). "Wire-loop application of liquid emulsion to slides for autoradiography in light microscopy". Stain ...
Bacteriophage T4 replicating DNA was labeled with tritiated thymidine and examined by autoradiography. The observed DNA ...
Then, in 1971, glycine was found to be localized in the spinal cord using autoradiography. All of these discoveries resulted in ... Hökfelt, Tomas; Ljungdahl, Åke (September 1971). "Light and electron microscopic autoradiography on spinal cord slices after ...
These could be fractionated by electrophoresis on a polyacrylamide gel and visualised using autoradiography. The procedure ...
... parallel observations from diffusion spectrum imaging and autoradiography". Brain. 130 (Pt 3): 630-53. doi:10.1093/brain/awl359 ...
These applications in most cases involve the tracing of implanted radioactive markers by Autoradiography. Examples are Medical ... such as autoradiography in medicine and biology. For a comprehensive and technically detailed account of the subject refer to ...
New York: Cambridge University Press, 1998) Ainsworth, M.W.; et al.: Art and Autoradiography: Insights into the Genesis of ...
... an autoradiography study". The Journal of Pharmacy and Pharmacology. 58 (9): 1243-8. doi:10.1211/jpp.58.9.0011. PMID 16945183. ...
Blue Devil Film is used for blotting, sequencing, chemiluminescent autoradiography (ecl) and gel shift analysis. It is designed ... Genesee Scientific has also been responsible for developing Blue Devil autoradiography film which produces exceptional clarity ... Blue Devil provides film is used for autoradiography, Western blotting, sequencing, chemiluminescence and gel shift analysis. ...
Recent number of edits (within past 90 days ...
"Autoradiography" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are MeSH descriptors whose meaning is more general than "Autoradiography".. *Analytical, Diagnostic and Therapeutic ... This graph shows the total number of publications written about "Autoradiography" by people in UAMS Profiles by year, and ... Below are the most recent publications written about "Autoradiography" by people in Profiles over the past ten years. ...
techniques of autoradiography second revised and enlarged edition P372》由于是年代较久的资料都绝版了,几乎不可能购买到实物。如果大家为了学习确实需要,博主可为大家寻取其电子版PDF文件 ... TECHNIQUES OF AUTORADIOGRAPHY》 1973 ELSEVIER SOIENTIFIC PUBLISHING COMPANY *《Computer Programming for Engineers Theory and ... TECHNIQUES OF AUTORADIOGRAPHY. 1973. MODERN MACHINE SHOP CONSTRUCTION EQUIPMENT AND MANAGEMENT SECOND EDITION REVISED
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Autoradiography involves localization, recording of a radiolabel within a solid specimen, and production of image in a ... Autoradiography can be broadly classified as direct and indirect autoradiography.. An autoradiograph is an image recorded on a ... Applications of Autoradiography. The autoradiography is used for the detection of the radiolabeled products since the 1950s. ... Digital Autoradiography. Resolution of activation autoradiography is done by digital computers and several technical means of ...
4.6.2. Autoradiography. Samples were resolved on 4-12% Bis-Tris NuPAGE gel at constant voltage (120 V) using NuPAGE MOPS SDS ...
A8813 Autoradiography Film BluLite™ 5x7 Film Blu-Lite™ UHC Autoradiography film, 5x7in, 100 sheets/box ... A8815 Autoradiography Film BluLite™ 8x10 Film Blu-Lite™ UHC Autoradiography film, 8x10in, 100 sheets/box ... A8803 Autoradiography Film BluBlot™ 5x7 Film BluBlot™ HS Autoradiography film, 5x7in, 100 sheets/box ... A8805 Autoradiography Film BluBlot™ 8x10 Film BluBlot™ HS Autoradiography film, 8x10in, 100 sheets/box ...
Text; Format: print Publication details: Paris : INSERM, 1990Availability: Items available for loan: WHO HQ (1)Call number: QH 585 90CA. ...
These in vivo data are also in discordance with our in vitro autoradiography results in the same region, i.e., a low level of ... Autoradiography Studies. The brain of one cat was extracted after euthanasia obtained by short inhalation of isoflurane and in ... Ex vivo autoradiography studies in rat brain revealed a significant binding reduction following d-fenfluramine iv pre- ... Radja, F., Laporte, A. M., Daval, G., Vergé, D., Gozlan, H., and Hamon, M. (1991). Autoradiography of serotonin receptor ...
Autoradiography is an imaging technique that is used to detect radioactive materials. Autoradiography is one of the most ...
... high sensitivity autoradiography film. Blu-Lite UHC film is specially formulated for an optimized balance of high sensitivity ... high sensitivity autoradiography film. Blu-Lite UHC film is specially formulated for an optimized balance of high sensitivity ... Western Blotting BluBlot™ HS autoradiography film 5x7in. Home › Western Blotting BluBlot™ HS autoradiography film 5x7in ... Blu-Lite UHC™ is MTC Bios newest line of high contrast, high sensitivity autoradiography film. Blu-Lite UHC film is specially ...
Bel-Art™ Autoradiography Pens Easy marking of film with nontoxic and nonradioactive phosphorescent material ...
7). Autoradiography and IHC demonstrated uptake of 89Zr-DFO-AMG102 largely in the stroma and areas of vasculature (basement ... Autoradiography and Histology. After PET imaging and excision of tumors, a subset of tumors was embedded in optimal-cutting- ... Similar autoradiography and immunofluorescence staining were performed on PDX tumor sections, which confirmed low uptake of 89 ... PET imaging, biodistribution, autoradiography and immunohistochemistry, and ex vivo HGF ELISA experiments were performed on ...
Autoradiography: Past and Emerging Technologies to Map Receptors/Enzymes and Their Functions ...
After 60 min, samples of brain and peripheral tissue were studied by autoradiography or HPLC. Purified nuclear pellets were ...
Alpha autoradiography has generally been used to detect the microdistribution of 10B in a cell. Although it has been performed ... Evaluation of thermal neutron irradiation field using a cyclotron-based neutron source for alpha autoradiography. Applied ... neutron source in combination with a water phantom to produce a thermal neutron irradiation field for alpha autoradiography. ...
The Autoradiography of Human Gliomas. Neuro-Oncology 1984, 2, 276-276. * Perry RH, Candy JM, Perry EK, Thompson J, Oakley AE. ... An autoradiography study using [I-125]alpha-conotoxinMII in the striatum and thalamus. Experimental Neurology 2005, 191(2), 292 ... Glutamate receptor autoradiography in human development and ageing: CNQX and MK-801 in the cerebellum. Journal of ... including autoradiography of hemispheric brain slices; identification of serotonergic, monoaminergic, and gabergic deficits in ...
Opioid receptors in midbrain dopaminergic regions of the rat I. MU receptor autoradiography. / German, D. C.; Speciale, S. G.; ... Opioid receptors in midbrain dopaminergic regions of the rat I. MU receptor autoradiography. In: Journal of Neural Transmission ... Dive into the research topics of Opioid receptors in midbrain dopaminergic regions of the rat I. MU receptor autoradiography ... title = "Opioid receptors in midbrain dopaminergic regions of the rat I. MU receptor autoradiography", ...
Gamma-activation autoradiography is a prospective method for screening detection of inclusions of precious metals in ... Development of digital gamma-activation autoradiography for analysis of samples of large area. ... The developed solutions considerably expand the possible applications of digital gamma-activation autoradiography. ...
Autoradiography and signal quantitation.. After ISH, all slides were arranged in x-ray cassettes and apposed to BioMax film ( ... 2001) Autoradiography of L-type and N-type calcium channels in aged rat hippocampus, entorhinal cortex, and neocortex. ...
Histology and Autoradiography. The subcutaneous xenografts from the mice of the PET and NIRF imaging cohort were excised in ... digital autoradiography. Lower section: magnified areas of the tumor slices corresponding to the white boxes in the top left ...
Autoradiography (ARG) is a high-resolution imaging method for localization of radiolabeled biomarkers in ex vivo specimen. ARG ... Digital autoradiography for efficient functional imaging without anesthesia in experimental animals: Reversing phencyclidine- ...
A new method to localize active renin in tissues by autoradiography: application to dog kidney.. ... Dry film autoradiography revealed discrete binding of 125I-H77 to the vascular pole of glomerulus as well as diffuse binding to ... A method was developed to localize active renin in dog kidney sections using autoradiography to detect in vitro binding of the ... Light microscopic emulsion autoradiography revealed highly selective discrete labelling of the juxtaglomerular apparatus. ...
Bergman K (1983). Application and results of whole-body autoradiography in distribution studies of organic solvents. CRC Crit ...
Visualization of a novel serotonin recognition site (5-HT1D) in the human brain by autoradiography. *C. Waeber, M. Dietl, D. ...
Autoradiography. Localization of ligand binding sites in frozen human tissue sections. These studies allow you to:. *Validate ...
  • Sections through the rostral-caudal extent of the midbrain were stained with an antibody against tyrosine hydroxylase, as a DA cell marker, and comparable sections were processed for in vitro receptor autoradiography using the mu-selective ligand, 3 H-Tyr-D-Ala-N-MePhe-Gyl-ol enkephalin. (
  • A method was developed to localize active renin in dog kidney sections using autoradiography to detect in vitro binding of the radiolabeled renin inhibitor, 125I-H77. (
  • These results demonstrate a new method to localize active renin in tissues using in vitro autoradiography and radioinhibitor binding. (
  • In this study we applied a pig model of renovascular hypertension to investigate the AT1R in vivo with positron-emission tomography (PET) and in vitro with quantitative autoradiography. (
  • Increased binding of [C]KR31173 documented by PET in vivo was confirmed by in vitro autoradiography. (
  • Neuroanatomical patterns of the m, s and k opiod receptors of rat brain as determined by quntitative in vitro autoradiography. (
  • In recent years, there has been an increasing interest in the use of whole body autoradiography (WBA), to study absorption, distribution, metabolism, and elimination (ADME) of drugs, which is done by image analysis. (
  • In this study whole-body autoradiography was used to ascertain the distribution of radioactivity after injection of MCPA in pregnant mice. (
  • Light microscopic emulsion autoradiography revealed highly selective discrete labelling of the juxtaglomerular apparatus. (
  • Receptor autoradiography is used to construct high-resolution maps of the receptor distributions and to examine relationships between specific cellular pathologies and changes in the densities of the receptor. (
  • Autoradiography involves localization, recording of a radiolabel within a solid specimen, and production of image in a photographic emulsion. (
  • Digital autoradiography provides information of accurate localization of signal with a better resolution, precise quantification, and possibility to quantify regions of interest. (
  • Autoradiography (ARG) is a high-resolution imaging method for localization of radiolabeled biomarkers in ex vivo specimen. (
  • 1971). In another study, the distribution of 14 C-labelled urea after intraperitoneal injection was determined by radioactivity analysis and autoradiography techniques in the brain and spinal fluid of fasted cats. (
  • PET imaging, biodistribution, autoradiography and immunohistochemistry, and ex vivo HGF ELISA experiments were performed on murine xenografts of U87MG (HGF-positive, MET-positive) and MKN45 (HGF-negative, MET-positive) and 4 patient-derived xenografts (MET-positive, HGF unknown). (
  • Unchanged tracer was measured in plasma and brain structures in four animals 10 and 30 min after injection, and ex-vivo autoradiography was also performed. (
  • Labeling detected by the imagers in digital autoradiography is similar to SPECT and PET, but autoradiograph requires a preparation of tissue sections. (
  • After 60 min, samples of brain and peripheral tissue were studied by autoradiography or HPLC. (
  • 70% of the signal in the brain, which resulted in well-defined regional binding on autoradiography. (
  • Autoradiography is an imaging technique that is used to detect radioactive materials. (
  • Alpha autoradiography has generally been used to detect the microdistribution of 10B in a cell. (
  • Autoradiography" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (
  • Basic autoradiography procedure (protocol) involves following steps as shown below. (
  • Blu-Lite UHC™ is MTC Bio's newest line of high contrast, high sensitivity autoradiography film. (
  • The autoradiography is used for the detection of the radiolabeled products since the 1950s. (
  • Autoradiography, Radioactive Isotope Detection eq. (
  • Digital autoradiography is used for the analysis of biological samples and for studying the process of drug metabolism. (
  • Results of search for 'su:{Autoradiography. (
  • Dry film autoradiography revealed discrete binding of 125I-H77 to the vascular pole of glomerulus as well as diffuse binding to the outer medulla and to the cortex. (
  • Autoradiography is used to study bacterial conjugation, and DNA sequencing (Southern blotting). (
  • A comparative study of DNA repair in fibroblast of normal person and patient suffering from xenoderma pigmentosum was done by semi-automated autoradiography. (
  • Track autoradiography is applied in diagnostics to study sealed radiation sources with low activities and emission of α particles. (
  • Autoradiography is one of the most important modern cytochemical methods to study the synthesis of molecules and trace the metabolic events in the cells . (
  • 14. Anil N, Hekimoglu C, Buyukbas N, Ercan MT. Microleakage study of various soft denture liners by autoradiography: effect of accelerated aging. (
  • Resolution of activation autoradiography is done by digital computers and several technical means of signals treatments. (
  • A new method to localize active renin in tissues by autoradiography: application to dog kidney. (
  • Autoradiography showed dense areas in the cerebral and cerebellar cortex. (
  • This graph shows the total number of publications written about "Autoradiography" by people in UAMS Profiles by year, and whether "Autoradiography" was a major or minor topic of these publications. (
  • The resource provides access to time lapse and 3-D microscopy and quantitative autoradiography. (
  • Quantitative autoradiography has been used to localize sites at which L-[3H]-glutamate is displaced by NMDA. (
  • X-ray micro computed tomography (X-mu CT) has proven to be a powerful tool for characterizing the spatial mineral distribution of geological samples in 3-D. However, limitations in resolution prevent an accurate characterization of pore space especially for tight crystalline rock samples and 2-D methods such as C-14-polymethylmethacrylate (C-14-PMMA) autoradiography and scanning electron microscopy (SEM) are needed. (
  • Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. (
  • Tyrosine hydroxylase TH-positive neurons in the substantia nigra and DAT and dopamine D 2 receptor (D 2 R) levels in the striatum were detected by immunohistochemical staining and/or autoradiography. (
  • Cortical and hippocampal AT1 receptor expression will be assessed using in vitro receptor autoradiography. (
  • Under optimal methodological conditions, UMB-1 immunohistochemistry may be equivalent to in vitro receptor autoradiography. (
  • strong class="kwd-title" Keywords: Somatostatin receptors, monoclonal anti-sst2A antibody, neuroendocrine tumors, immunohistochemistry, somatostatin receptor autoradiography Introduction Somatostatin receptors signify molecular tumor focuses on of increasing scientific importance (17). (
  • The gold standard THZ1 inhibition method to do this is in vitro somatostatin receptor autoradiography. (
  • Whole-body autoradiography of histamine-14-C in rats. (
  • Absorption appeared to be mainly in the stomach when whole body autoradiography was performed. (
  • Cryosections of the tumor bearing brains were analyzed by ex vivo autoradiography and immunofluorescence staining for blood vessels, microglia, astrocytes, and presence of PSMA. (
  • We will perform ex vivo biodistribution, in vitro autoradiography and histopathological assays to correlate microtubule density with routine neurobiomarkers. (
  • Alternatively, enhanced chemiluminescence followed by autoradiography can be used to visualize the multimers. (
  • Example: combination of light microscopic staining methods or autoradiography with electron microscopy. (
  • Autoradiography films, electron microscopy films, emulsions and imaging accessories for life science research. (
  • Goodman, RR & Snyder, SH 1982, ' κ opiate receptors localized by autoradiography to deep layers of cerebral cortex: Relation to sedative effects ', Proceedings of the National Academy of Sciences of the United States of America , vol. 79, no. 18 I, pp. 5703-5707. (
  • An estimate of the length of the DNA molecule of T2 bacteriophage by autoradiography. (
  • The application of high-speed autoradiography to histologic brain sections of in vivo [ 3 H]thymidine labelled rats is reported. (
  • Ex vivo imaging, autoradiography, histologic studies and immunofluorescence staining were also conducted to validate the results of in vivo imaging. (
  • The exact location of the 68Ga-FAPI-04 uptake was confirmed by ex vivo imaging, as well as autoradiography and histologic findings. (
  • The detected RFLP is visualized using X-ray film in autoradiography, where DNA fragments can be viewed and analyzed after they are separated from one another by electrophoresis. (
  • Autoradiography Films is widely used for Blotting, Sequencing and other. (
  • GenHunter offers high-quality autoradiography film for most applications including Western Blotting. (
  • The X-mu CT were used to characterize the spatial distribution of the main minerals in 3-D. Total porosities, fracture porosities, fracture densities and porosity distributions of the samples were determined using the C-14-PMMA autoradiography and characterization of mineral-specific porosities were assisted using chemical staining of rock surfaces. (
  • Both parts of my PhD work and my current research focuses specifically on employing advanced digital autoradiography, small-scale dosimetry, in vivo imaging, etc for radioimmunotherapy research. (
  • 15 followed the fate of proliferating ATII cells (after 48 h of NO 2 exposure) using a combination of autoradiography and electron microscopic analysis. (
  • For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. (
  • Autoradiography is the process of taking a type of picture, called an autoradiograph, which shows the relative concentration of radioactive material present within the subject. (
  • The photographic plate is exposed to radioactive emissions from the subject being studied, producing an image.The global average price of Autoradiography Films is in the decreasing trend, from 1.52 USD/Sheet in 2012 to 1.43 USD/Sheet in 2016. (
  • The autoradiography technique consists in depositing sensitive screens collecting the radiation on facility floors, ceilings or walls during different exposure times. (
  • Autoradiography showed localized uptake in intracranial U87 and U251 cells transduced with Ad.TK. (
  • In more detailed studies using autoradiography with 125I-RTI-55, recovery of serotonin uptake sites varied from region to region. (
  • C) Autoradiography image-derived infarct-to-noninfarct uptake ratio (derived from 3 nonblocked and 3 blocked MI hearts). (
  • After 18-20 h of exposure, the cells in the autoradiography dishes were washed in serum free WME, swelled in 1% sodium citrate and fixed in ethanol-glacial acetic acid fixative. (
  • Yrjö Collan , «Studying cells with different methods in serial sections: principles of matching», Acta Stereologica [En ligne], Volume 6 (1987), Supplement II (The commemorative-memorial volume: twenty-five years of stereology) - May 1987, 157-167 URL : (
  • Some autoradiographs can be examined microscopically for localization of silver grains (such as on the interiors or exteriors of cells or organelles) in which the process is termed micro-autoradiography. (
  • The global Autoradiography Films market was valued at 90.04 Million USD in 2021 and will grow with a CAGR of 2.68% from 2021 to 2027, based on HNY Research newly published report. (
  • GenHunter's PerfectFilm is a high-quality, especially sensitive blue autoradiography film that is ideal for most applications including Western blots using ECL substrates for HRP or AP! (
  • Part II: Radioligand binding and autoradiography studies. (
  • Autoradiography demonstrated a strong 68 Ga-PSMA and 18 F-DCFPyL binding in the peritumoral area and moderate binding in the center of the tumors. (
  • Tagged samples had been solved on 8% Tricine polyacrylamide gels, used in PVDF membrane and visualized by autoradiography. (
  • Aside from requiring a large amount of sample DNA--the sample would usually need to be about the size of a quarter, which is relatively large for DNA samples--the process, from probe labeling to washing and autoradiography, can take up to a full month to complete. (
  • North America is the largest supplier of Autoradiography Films, with a production market share nearly 43% in 2016. (
  • Asia Pacific is the second largest supplier of Autoradiography Films Media, enjoying production market share nearly 29% in 2016. (
  • For their D&D projects, CEA LASE Laboratory has developed the Digital Autoradiography technique to get images of radionuclides that can be found in nuclear wastes but difficult to measure. (
  • Combining Digital Autoradiography and Kartotrak allowed identifying hot spots on the ground of a laboratory that hosted nuclear researches and that must be dismantled. (
  • Alternatively, the autoradiograph is also available as a digital image (digital autoradiography), due to the recent development of scintillation gas detectors or rare earth phosphorimaging systems. (
  • periodic acid-Schiff staining was performed, and samples were subjected to autoradiography. (
  • Autoradiography visualises their positions while PhosphoImaging is used for their quantification. (
  • Vélocimétrie Doppler : applications en pharmacologie cardiovasculaire animale et clinique / edité par Pierre Péronneau. (