The spontaneous disintegration of tissues or cells by the action of their own autogenous enzymes.
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
An autolytic enzyme bound to the surface of bacterial cell walls. It catalyzes the hydrolysis of the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell wall glycopeptides, particularly peptidoglycan. EC 3.5.1.28.
Cysteine proteinase found in many tissues. Hydrolyzes a variety of endogenous proteins including NEUROPEPTIDES; CYTOSKELETAL PROTEINS; proteins from SMOOTH MUSCLE; CARDIAC MUSCLE; liver; platelets; and erythrocytes. Two subclasses having high and low calcium sensitivity are known. Removes Z-discs and M-lines from myofibrils. Activates phosphorylase kinase and cyclic nucleotide-independent protein kinase. This enzyme was formerly listed as EC 3.4.22.4.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
The inactive proenzyme of trypsin secreted by the pancreas, activated in the duodenum via cleavage by enteropeptidase. (Stedman, 25th ed)
Proteins found in any species of bacterium.
Bacterial polysaccharides that are rich in phosphodiester linkages. They are the major components of the cell walls and membranes of many bacteria.
Physiological changes that occur in bodies after death.
One of the CEPHALOSPORINS that has a broad spectrum of activity against both gram-positive and gram-negative microorganisms.
A specialized proteolytic enzyme secreted by intestinal cells. It converts TRYPSINOGEN into its active form TRYPSIN by removing the N-terminal peptide. EC 3.4.21.9.
Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
Nonionic surfactant mixtures varying in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups. They are used as detergents, emulsifiers, wetting agents, defoaming agents, etc. Octoxynol-9, the compound with 9 repeating ethoxy groups, is a spermatocide.
A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
Proteins from the nematode species CAENORHABDITIS ELEGANS. The proteins from this species are the subject of scientific interest in the area of multicellular organism MORPHOGENESIS.
The predominant milk-clotting enzyme from the true stomach or abomasum of the suckling calf. It is secreted as an inactive precursor called prorennin and converted in the acid environment of the stomach to the active enzyme. EC 3.4.23.4.
A mixture of related phosphoproteins occurring in milk and cheese. The group is characterized as one of the most nutritive milk proteins, containing all of the common amino acids and rich in the essential ones.
Services providing counseling and activities that help overweight individuals to attain a more healthy body weight.
A malignant tumor derived from primitive or embryonal lipoblastic cells. It may be composed of well-differentiated fat cells or may be dedifferentiated: myxoid (LIPOSARCOMA, MYXOID), round-celled, or pleomorphic, usually in association with a rich network of capillaries. Recurrences are common and dedifferentiated liposarcomas metastasize to the lungs or serosal surfaces. (From Dorland, 27th ed; Stedman, 25th ed)
A cephalosporin antibiotic.
A group of antibiotics that contain 6-aminopenicillanic acid with a side chain attached to the 6-amino group. The penicillin nucleus is the chief structural requirement for biological activity. The side-chain structure determines many of the antibacterial and pharmacological characteristics. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1065)
Exotoxins produced by certain strains of streptococci, particularly those of group A (STREPTOCOCCUS PYOGENES), that cause HEMOLYSIS.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
Antibiotic substance produced by Streptomyces garyphalus.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)
The application of genetic analyses and MOLECULAR DIAGNOSTIC TECHNIQUES to legal matters and crime analysis.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles.
The physical or mechanical action of the LUNGS; DIAPHRAGM; RIBS; and CHEST WALL during respiration. It includes airflow, lung volume, neural and reflex controls, mechanoreceptors, breathing patterns, etc.
The branch of psychology which seeks to learn more about the fundamental causes of behavior by studying various psychologic phenomena in controlled experimental situations.
The volume of air inspired or expired during each normal, quiet respiratory cycle. Common abbreviations are TV or V with subscript T.
The exchange of OXYGEN and CARBON DIOXIDE between alveolar air and pulmonary capillary blood that occurs across the BLOOD-AIR BARRIER.
A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.
Fatty acid biopolymers that are biosynthesized by microbial polyhydroxyalkanoate synthase enzymes. They are being investigated for use as biodegradable polyesters.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.
A gram-negative, facultatively chemoautotrophic bacterium, formerly called Wautersia eutropha, found in water and soil.
A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.
Proteins found in any species of fungus.
Fermented juice of fresh grapes or of other fruit or plant products used as a beverage.
A plant species of the family CUCURBITACEAE, order Violales, subclass Dilleniidae known for the melon fruits with reticulated (net) surface including cantaloupes, honeydew, casaba, and Persian melons.
The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds.
The gourd plant family of the order Violales, subclass Dilleniidae, class Magnoliopsida. It is sometimes placed in its own order, Cucurbitales. 'Melon' generally refers to CUCUMIS; CITRULLUS; or MOMORDICA.
A family of SERINE ENDOPEPTIDASES isolated from Bacillus subtilis. EC 3.4.21.-
Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.

Autolysosomal membrane-associated betaine homocysteine methyltransferase. Limited degradation fragment of a sequestered cytosolic enzyme monitoring autophagy. (1/238)

We compared the membrane proteins of autolysosomes isolated from leupeptin-administered rat liver with those of lysosomes. In addition to many polypeptides common to the two membranes, the autolysosomal membranes were found to be more enriched in endoplasmic reticulum lumenal proteins (protein-disulfide isomerase, calreticulin, ER60, BiP) and endosome/Golgi markers (cation-independent mannose 6-phosphate receptor, transferrin receptor, Golgi 58-kDa protein) than lysosomal membranes. The autolysosomal membrane proteins include three polypeptides (44, 35, and 32 kDa) whose amino-terminal sequences have not yet been reported. Combining immunoblotting and reverse transcriptase-polymerase chain reaction analyses, we identified the 44-kDa peptide as the intact subunit of betaine homocysteine methyltransferase and the 35- and 32-kDa peptides as two proteolytic fragments. Pronase digestion of autolysosomes revealed that the 44-kDa and 32-kDa peptides are present in the lumen, whereas the 35-kDa peptide is not. In primary hepatocyte cultures, the starvation-induced accumulation of the 32-kDa peptide occurs in the presence of E64d, showing that the 32-kDa peptide is formed from the sequestered 44-kDa peptide during autophagy. The accumulation is induced by rapamycin but completely inhibited by wortmannin, 3-methyladenine, and bafilomycin. Thus, detection of the 32-kDa peptide by immunoblotting can be used as a streamlined assay for monitoring autophagy.  (+info)

Developmental aspects of secondary palate formation. (2/238)

Research on development of the secondary palate has, in the past, dealt primarily with morphological aspects of shelf elevation and fusion. The many factors thought to be involved in palatal elevation, such as fetal neuromuscular activity and growth of the cranial base and mandible, as well as production of extracellular matrix and contractile elements in the palate, are mostly based on gross, light microscopic, morphometric or histochemical observations. Recently, more biochemical procedures have been utilized to described palatal shelf elevation. Although these studies strongly suggest that palatal extracellular matrix plays a major role in shelf movement, interpretation of these data remains difficult owing to the complexity of tissue interactions involved in craniofacial development. Shelf elevation does not appear to involve a single motive factor, but rather a coordinated interaction of all of the abovementioned developmental events. Further analysis of mechanisms of shelf elevation requires development of new, and refinement of existing, in vitro procedures. A system that enables one to examine shelf elevation in vitro would allow more meaningful analysis of the relative importance of the various components in shelf movement. Much more is known about fusion of the palatal shelves, owing in large part to in vitro studies. Fusion of the apposing shelves, both in vivo and in vitro, is dependent upon adhesion and cell dealth of the midline epithelial cells. Adhesion betweeen apposing epithelial surfaces appears to involve epithelial cell surface macromolecules. Further analysis of palatal epithelial adhesion should be directed towards characterization of those cell surface components responsible for this adhesive interaction. Midline epithelial cells cease DNA synthesis 24-36 h before shelf elevation and contact, become active in the synthesis of cell surface glycoproteins, and subsequently manifest morphological signs of necrosis. Death of the midline epithelial cells is thought to involve a programmed, lysosomal-mediated autolysis...  (+info)

Suppression of the lytic and bactericidal effects of cell wallinhibitory antibiotics. (3/238)

The bacteriolytic effect of beta-lactam antibiotics on Bacillus subtilis and on Streptococcus pneumoniae was found to be a function of the pH; lysis was suppressed if the pH of the pneumococcal culture was below 6.0 during penicillin treatment. In the case of B. subtilis, growth at pH 6.6 prevented penicillin-induced lysis. In pneumococci, the addition of trypsin to the growth medium also protected against lysis. The pH-dependent protection phenomenon resembled in several respects the antibiotic "tolerance" of pneumococci with a defective autolytic system. (i) At the pH nonpermissive for lysis, the bacteria retained their normal sensitivity to beta-lactam and to other cell wall inhibitors; however, instead of lysis, the drug-treated bacteria simply stopped growing. Loss of viability of the cells was also greatly reduced. (ii) Protection against lysis was independent of the dose and chemical nature of the cell wall inhibitors. (iii) The protection effect was reversible; lysis and loss of viability could be triggered by a postincubation of the drug-treated bacteria at the pH permissive for lysis.  (+info)

The autolysis loop of activated protein C interacts with factor Va and differentiates between the Arg506 and Arg306 cleavage sites. (4/238)

The anticoagulant human plasma serine protease, activated protein C (APC), inactivates blood coagulation factors Va (FVa) and VIIIa. The so-called autolysis loop of APC (residues 301-316, equivalent to chymotrypsin [CHT] residues 142-153) has been hypothesized to bind FVa. In this study, site-directed mutagenesis was used to probe the role of the charged residues in this loop in interactions between APC and FVa. Residues Arg306 (147 CHT), Glu307, Lys308, Glu309, Lys311, Arg312, and Arg314 were each individually, or in selected combinations, mutated to Ala. The purified recombinant protein C mutants were characterized using activated partial thromboplastin time (APTT) clotting assays and FVa inactivation assays. Mutants 306A, 308A, 311A, 312A, and 314A had mildly reduced anticoagulant activity. Based on FVa inactivation assays and APTT assays using purified Gln506-FVa and plasma containing Gln506-FV, it appeared that these mutants were primarily impaired for cleavage of FVa at Arg506. Studies of the quadruple APC mutant (306A, 311A, 312A, and 314A) suggested that the autolysis loop provides for up to 15-fold discrimination of the Arg506 cleavage site relative to the Arg306 cleavage site. This study shows that the loop on APC of residues 306 to 314 defines an FVa binding site and accounts for much of the difference in cleavage rates at the 2 major cleavage sites in FVa. (Blood. 2000;96:585-593)  (+info)

The D-alanine residues of Staphylococcus aureus teichoic acids alter the susceptibility to vancomycin and the activity of autolytic enzymes. (5/238)

Recently, Staphylococcus aureus strains with intermediate resistance to vancomycin, the antibiotic of last resort, have been described. Multiple changes in peptidoglycan turnover and structure contribute to the resistance phenotype. Here, we describe that structural changes of teichoic acids in the cell envelope have a considerable influence on the susceptibility to vancomycin and other glycopeptides. S. aureus cells lacking D-alanine esters in teichoic acids exhibited an at least threefold-increased sensitivity to glycopeptide antibiotics. Furthermore, the autolytic activity of the D-alanine mutant was reduced compared to the wild-type, and the mutant was more susceptible to the staphylolytic enzyme lysostaphin. Vancomycin inhibited autolysis at very high concentrations but neither in the wild-type nor in the mutant was the autolytic activity influenced in the range of the MIC. Mutant cells had a considerably higher capacity to bind vancomycin.  (+info)

Description of staphylococcus serine protease (ssp) operon in Staphylococcus aureus and nonpolar inactivation of sspA-encoded serine protease. (6/238)

Signature tagged mutagenesis has recently revealed that the Ssp serine protease (V8 protease) contributes to in vivo growth and survival of Staphylococcus aureus in different infection models, and our previous work indicated that Ssp could play a role in controlling microbial adhesion. In this study, we describe an operon structure within the ssp locus of S. aureus RN6390. The ssp gene encoding V8 protease is designated as sspA, and is followed by sspB, which encodes a 40.6-kDa cysteine protease, and sspC, which encodes a 12.9-kDa protein of unknown function. S. aureus SP6391 is an isogenic derivative of RN6390, in which specific loss of SspA function was achieved through a nonpolar allelic replacement mutation. In addition to losing SspA, the culture supernatant of SP6391 showed a loss of 22- to 23-kDa proteins and the appearance of a 40-kDa protein corresponding to SspB. Although the 40-kDa SspB protein could degrade denatured collagen, our data establish that this is a precursor form which is normally processed by SspA to form a mature cysteine protease. Culture supernatant of SP6391 also showed a new 42-kDa glucosaminidase and enhanced glucosaminidase activity in the 29 to 32 kDa range. Although nonpolar inactivation of sspA exerted a pleiotropic effect, S. aureus SP6391 exhibited enhanced virulence in a tissue abscess infection model relative to RN6390. Therefore, we conclude that SspA is required for maturation of SspB and plays a role in controlling autolytic activity but does not by itself exert a significant contribution to the development of tissue abscess infections.  (+info)

Increasing the thermal stability of euphauserase. A cold-active and multifunctional serine protease from Antarctic krill. (7/238)

A molecular model of Antarctic krill euphauserase based on the known crystal structure of its fiddler crab analog, collagenase I, indicates that the core structure of these enzymes is almost identical. Euphauserase is a cold-active and thermally sensitive enzyme with a high affinity for Lys, Arg and large hydrophobic amino acids. Residue Phe137 in euphauserase, localized in loop D (autolysis loop), is highly exposed on the surface of the molecule. Therefore, it appeared to be an easy target for autolysis. The broadly specific euphauserase has a low affinity for negatively charged residues. In order to increase the stability of the enzyme, two mutants were created in which residue Phe137 was replaced by a Glu and an Asp residue. Both mutations resulted in increased stability of the recombinant euphauserase towards thermal inactivation.  (+info)

The AbcA transporter of Staphylococcus aureus affects cell autolysis. (8/238)

Increased production of penicillin-binding protein PBP 4 is known to increase peptidoglycan cross-linking and contributes to methicillin resistance in Staphylococcus aureus. The pbp4 gene shares a 400-nucleotide intercistronic region with the divergently transcribed abcA gene, encoding an ATP-binding cassette transporter of unknown function. Our study revealed that methicillin stimulated abcA transcription but had no effects on pbp4 transcription. Analysis of abcA expression in mutants defective for global regulators showed that abcA is under the control of agr. Insertional inactivation of abcA by an erythromycin resistance determinant did not influence pbp4 transcription, nor did it alter resistance to methicillin and other cell wall-directed antibiotics. However, abcA mutants showed spontaneous partial lysis on plates containing subinhibitory concentrations of methicillin due to increased spontaneous autolysis. Since the autolytic zymograms of cell extracts were identical in mutants and parental strains, we postulate an indirect role of AbcA in control of autolytic activities and in protection of the cells against methicillin.  (+info)

Global Yeasts, Yeast Extracts and Autolysates Market is valued at $6.65 billion in 2014 and is poised to reach $12.54 billion by 2022 growing at a CAGR of...
The trypsin used was side-chain protected porcine trypsin from Promega. The enzyme was derivatized by reductive methylation (Lys side-chains) to prevent autolysis. The data suggest that it underwent some autolysis prior to and/or during the derivatization. The peptides in bold were identified by LC/MS/MS experiments. The Cys residues participate in disulfide-linkages. The list contains only N-terminally methylated or doubly methylated or Lys side-chain Me-derivatives. ...
Dive into the research topics of ADAM19 autolysis is activated by LPS and promotes non-classical secretion of cysteine-rich protein 2. Together they form a unique fingerprint. ...
Cysteine protease involved in xylem tracheary element (TE) autolysis during xylogenesis in roots. Participates in micro autolysis within the intact central vacuole before mega autolysis is initiated by tonoplast implosion.
Elucidating the origin of the surface functionalization - dependent bacterial toxicity of graphene nanomaterials: Oxidative damage, physical disruption, and cell autolysis ...
Activation of skeletal muscle calpain-3 by eccentric exercise in humans does not result in its translocation to the nucleus or cytosol. J Appl Physiol (1985). 2011 Nov; 111(5):1448-58 ...
The surface charge of yeast cells was found to be determined by the design and parameters of the fermentation process. Cells may be emptied by autolysis/plasmolysis, extraction methods which have proven effective in industry as well, to obtain hollow yeast cell envelopes comprised of cell walls only. The extraction processes render the yeast cells inactive and transform them into yeast cell envelopes, washing out intracellular low-molecular substances whilst maintaining the shape of the cells. Even though the cell�s zeta potential � main criterion for assessing their suitability for coating in subsequent precipitation processes � goes down during autolysis/plasmolysis, it is still sufficient for the requirements of this application ...
Words starting with A (page 114): autokinesis, autokinetic, autolatries, autolatry, autoload, autoloader, autoloading, autologies, autologous, autology, autolysate, autolyse, autolysed, autolyses, autolysin, autolysing, autolysins, autolysis, autolytic, autolyzate...
Defects in the formation of the septum and gradually autolysis of cells occur when the dapdependent mutant ofEscherichia coli is grown in a medium with 4-hydroxy-diaminopimelic... ...
The potential of the application of PEF to induce accelerate autolysis of a commercial strain of Sacharomyces cerevisiae for winemaking use was evaluated. The influence of PEF treatments of different intensity (5-25 kV/cm for 30-240 µs) on cell viability, cytoplasmic membrane permeabilization, release of mannoproteins and compounds absorbing at 260 and 280 nm has been investigated.After 8 days of incubation at 25 ºC the Abs600 of the suspension containing the control cells was kept constant while the Abs600 of the suspension containing the cells treated by PEF decreased. The measurement of the absorbance at 260 and 280 nm revealed no release of UV absorbing material from untreated cells after 8 days of incubation but the amount of UV absorbing material released drastically increased in the samples that contained cells treated by PEF after the same storage period. After 18 days of storage the amount of mannoproteins released from the untreated cell was negligible. Conversely, mannoprotein concentration
China The reason of beer yeast autolysis Suppliers,products - The fermentation process is actually the yeast metabolism process, to obtain high-quality beer, must first have a strong vitality, its own strong, good performance, flavor beneficial yeast strains. And yeast performance by the fermentation process conditions and the appearance of environmental facto...
05 Proline The dominating amino acids in both cropping types were cysteic acid, citrulline, proline, phenylalanine, and taurine. 7). An accumulation of proline under continuous cropping system was reported by Życzyńska-Bałoniak et al. (1986). The accumulation of proline under continuous cropping of rye conditions may be due to its heterocyclic amino acid structure, which could be more resistant to microbial degradation than other amino acids. The accumulation of proline in soils is considered as negative effect. The polymers of HA are synthesized enzymatically within microbial cell by chemical oxidative condensation following cellular autolysis. Therefore, HA are rarely homogenous; however, they represent accumulation of more resistant end products from many reaction taking place under natural conditions, either directly or indirectly through biological processes. ) in which it is formed (Piccolo et al. 2003). HA provides energy to many biochemical processes in soil (Dziadowiec 1979; ...
1. The administration of dihydrotestosterone to rats orchidectomized 7 days previously stimulated the synthesis of nuclear receptor in prostatic cells several hours in advance of DNA synthesis and mitosis. 2. The synthesis of nuclear receptor is tightly coupled to cell proliferation; consequently, in resting cells, there is no further net synthesis of nuclear receptor above the maximum of approx. 8000 molecules/cell. 3. After orchidectomy a rapid decline in the concentration of free androgen in the nuceus and a slower decline in the concentration of nuclear receptor are observed. 4. Owing to the apparent scarcity of receptor-inactivating factors in the nucleus, and the inverse relationship between amounts of nuclear and cytoplasmic receptors, it is concluded that the nuclear receptor is discharged into the cytoplasm after orchidectomy. 5. The formation of the cytoplasmic receptor is an early event preceding the onset of cellular autolysis. 6. Regressing prostate develops the capacity to ...
BCC Research (Wellesley; MA), a market research group, predicts the global market for yeast, yeast extract and autolysates will increase at a compound annual growth rate (CAGR) for 7.6% to $4.7...
Its all about the synonyms. Why say sweet when you can say residual malt sugars? Who needs alcoholic when you can discuss ABV? Brewers are shy and timid creatures really, and one word may be enough to gain their attention and respect if suitably technical. Swirl the glass around in your hand, sniff and grunt: Hmm. Hint of autolysis. Or: interesting - redolent of phenols. Or: spot of diacetyl. They will be in awe of your abilities. After a few drinks, the word you use wont even matter: frown, adopt a serious town, and say: Ah, theres a bit of dialysis in here. Everyone else will nod. You will end the evening either sounding like a knowledgeable critic or an intelligent enthusiast. Either way, its all good ...
vasokonstriksi  dilatasi  hemorraghia subepielial  membtk hematoma di lapisan superfisial endomet  rupturnya lapisan atas endomet  jaringan endomet rusak dilepas bersama darah sbg darah haid. Fragmen jaringan endomet dapat dikenali pd darah haid meskipun tjd autolysis  perdarahan berhenti bila arteri spiralis mengalami vasokonstriksi  permukaan endomet tertutup o/ epitel yg tumbuh dr sisa epitel kelenjar ...
vasokonstriksi  dilatasi  hemorraghia subepielial  membtk hematoma di lapisan superfisial endomet  rupturnya lapisan atas endomet  jaringan endomet rusak dilepas bersama darah sbg darah haid. Fragmen jaringan endomet dapat dikenali pd darah haid meskipun tjd autolysis  perdarahan berhenti bila arteri spiralis mengalami vasokonstriksi  permukaan endomet tertutup o/ epitel yg tumbuh dr sisa epitel kelenjar ...
The Human Factory has written and spoken worldwide about the underappreciated issue of health blindness, that around 6% of medical personnel are severely handicapped at seeing veins and clinical signs that others take for granted. Human Factory innovati
Yamagata H.; Ueno S.; Iwasaki T., 1989: Isolation and characterization of a possible native cucumisin from developing melon fruits and its limited autolysis to cucumisin
A cadaver, also called corpse (singular) in medical, literary, and legal usage, or when intended for dissection, is a deceased body. Observation of the various stages of decomposition can help determine how long a body has been dead. The first stage is autolysis, more commonly known as self-digestion, during which the bodys cells are destroyed through the action of their own digestive enzymes. However, these enzymes are released into the cells because of active processes ceasing in the cells, not as an active process. In other words, though autolysis resembles the active process of digestion of nutrients by live cells, the dead cells are not actively digesting themselves as is often claimed in popular literature and as the synonym of autolysis self-digestion seems to imply. As a result of autolysis, liquid is created that gets between the layers of skin and makes the skin peel off. During this stage, flies (when present) start to lay eggs in the openings of the body: eyes, nostrils, mouth, ...
We originally identified AtlA as a predicted surface protein that was required for maturation of biofilms (10). Disruption of atlA also resulted in resistance to autolysis and excessive chaining of cells (10, 69), and Shibata et al. (66) determined that the protein had peptidoglycan hydrolase activity in zymograms. Our present study reveals that AtlA is also required for biogenesis of a normal cell surface and full expression of genetic competence by S. mutans. Thus, AtlA appears to play a central role connecting cell wall remodeling, biofilm formation, genetic competence, and autolysis. These networks have often been shown to overlap with the stress regulon, but the AtlA protein does not appear to be required for acid tolerance, since no obvious differences were observed in the growth rate of the atlA mutant in acidified BHI broth (pH 6.4 or 5.4) (data not shown). However, it is possible that AtlA may be involved in other stress responses, because autolysin-mediated cell wall turnover is known ...
No, the science behind juice fasting is similiar to water fasting. In both ventures your body slips into a state of autolysis, a natural state of repair where digestion stops, which is why people don t die from fasting but will quickly die from starvation. Look into books like, The Juice Fasting Bible or How to keep Slim, Healthy and Young through Juice Fasting . Both were written by MDs.
Synonyms for autolytic in Free Thesaurus. Antonyms for autolytic. 1 synonym for autolysis: self-digestion. What are synonyms for autolytic?
Two courses of reinduction chemotherapy followed by bone marrow biopsy and aspirate prior to peripheral blood stem cell (PBSC) harvest. If marrow involvement is still present at harvest, then 2 additional courses of induction chemotherapy are given. The PBSC transplantation preparative regimen should begin within 2 weeks of completing reinduction therapy course, consisting of the following: Carmustine IV over 3 hours on days -8, -7, and -6, Etoposide continuous IV over days -8, -7, and -6, Cyclophosphamide IV over 1 hour daily on days -5, -4, -3, and -2, Mesna as a 15 min infusion before each dose of cyclophosphamide then at 3, 6, 9, and 12 hours after initiation of each cyclophosphamide dose Methylprednisolone IV is given to protect lungs from the toxic effects of carmustine ...
Minimise temperature shocks. If a refrigerant temperature of close to zero degrees Celsius is capable of controlling the fermentation process, it would certainly be of benefit to the growing yeast cells, which would not be subjected to temperature shock if the refrigerant were at a lower temperature. Similarly the cone temperature, which is often controlled at -4 degrees Celsius, could be contributing significantly to yeast autolysis at the interface. Consideration should also be given to a two-phase water chiller for reducing the temperature of recovered yeast, rather than the sudden reduction of temperature using propylene glycol or compressed ammonia at very low temperatures. The rate of chillback of the fermentation should also be prolonged to ensure that the possibility of temperature shock is minimised ...
Disclosed is a controlled autolysis method for making biological tissue substantially acellular by exposing the biological material, prior to any fixation thereof, to at least one buffered solution having a pH in the range from about 5.0 to 8.0 and a temperature in the range from about 12 C. to 30 C. for a sufficient period of time to render at least one region of the biological material substantially acellular while substantially preserving the structural integrity and non-cellular structural components of the biological material. Also disclosed is a method of making a bioprosthetic heart valve using biological material that has been treated by controlled autolysis and a method of treating a mammal having a defective heart valve using a bioprosthetic heart valve made, in part, by controlled autolysis.
Synonyms for Cell lysis in Free Thesaurus. Antonyms for Cell lysis. 19 words related to lysis: convalescence, recuperation, recovery, biochemistry, autolysis, self-digestion, bacteriolysis, cytolysis, dissolution, disintegration. What are synonyms for Cell lysis?
niger during submerged cultivation. The impact of secondary growth by carbon recycling was indicated by hyphal population dynamics illustrating a gradual transition from old to young hyphae. The induc tion of autophagic and reproductive processes was clearly evident by major genome wide transcriptional trends. Hydrolases with strong transcriptional induc tion during carbon starvation include ChiB, NagA, AgnB, PepA and PepB. Importantly, fragmentation of empty hyphal ghosts was not observed, thus constitut ing direct evidence that autolysis in aging submerged cultures of A. niger is rather initiated Inhibitors,Modulators,Libraries by cell death than by hydrolytic weakening and fragmentation of cell walls. Methods Strain, inoculum and media compositions Conidial suspensions for inoculation of bioreactor cul tures Inhibitors,Modulators,Libraries were prepared by growing the A.. niger laboratory strain N402 on solid i?ed complete medium for three days at 30 C in the dark. Spores were harvested with ...
niger during submerged cultivation. The impact of secondary growth by carbon recycling was indicated by hyphal population dynamics illustrating a gradual transition from old to young hyphae. The induc tion of autophagic and reproductive processes was clearly evident by major genome wide transcriptional trends. Hydrolases with strong transcriptional induc tion during carbon starvation include ChiB, NagA, AgnB, PepA and PepB. Importantly, fragmentation of empty hyphal ghosts was not observed, thus constitut ing direct evidence that autolysis in aging submerged cultures of A. niger is rather initiated Inhibitors,Modulators,Libraries by cell death than by hydrolytic weakening and fragmentation of cell walls. Methods Strain, inoculum and media compositions Conidial suspensions for inoculation of bioreactor cul tures Inhibitors,Modulators,Libraries were prepared by growing the A.. niger laboratory strain N402 on solid i?ed complete medium for three days at 30 C in the dark. Spores were harvested with ...
Cant believe it. In Russia we say u durakov mysli odinakovye (fools share their mind on air). I have the same article been opened maybe since Feb.2017 (and its opened just now). On one hand, the most of yeast is inactive after first 48 hours (being dead or sick, hehe). Due to its sickness (lo-sugars + hi-alco + progressing autolysis), the colony starts to make more and more by-products. But the U.S. tradition tends to have hi-nitro wort in the very beginning (sour mash or dunder or rum bomb + the non-filtered mash fermentation). And... I didnt taste much of American malt or corn based beverages but all I know of have put a long-long tail into my nose. In contrast to it, Islay is rich in powerful esters, maybe some feinty notes but not so definite tails. Longer aging only? Secret Gaelic strains? AFAIK, they filter the mash prior to pitching the yeast. No nitro-vitamin-bombs or porridge or backset inside. And SG 1.05..1.06. They make sorta ale without hops, in fact. But still there ...
An impeccable sample fixation is a prerequisite for a correct histological diagnosis. Tissue samples must be immersed in an optimally chosen fixative immediately after sampling, because a timely fixation will prevent autolysis, putrefaction and other unwanted cellular changes. Although there are hundreds of histological fixatives and at least tens of formaldehyde-based fixatives, neutral buffered formaldehyde solutions with a concentration range from 4% to 10% are the most commonly used fixatives, primarily because of their simple and universal application. Tissue fixation using a buffered formaldehyde solution results in forming cross-links, i. e. it forms methylene bridges between proteins, that is, it results in keeping tissue components in their in vivo relation. If fixated properly, the tissue sample can withstand additional histological tissue processing and staining.. The NB 4% formaldehyde solution is 10% buffered formalin, which is the most commonly used fixative. It is suitable for ...
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A coordinated action of murein synthetases and murein hydrolases is assumed to be essential for proper growth of the bacterial cell. A disturbance of this balance, for example by blocking parts of...
The acid-acting proteinase, cathepsin D (EC 3.4.4.23), was purified from extracts of homogenized rabbit lung and beef lung by autolysis at acid pH, acetone and ammonium sulfate fractionation, column chromatography, and isoelectric focusing. Four isoenzymes were obtained from each source. With acid hemoglobin as the substrate, the proteinase from rabbit lung had a pH optimum of 3.0 and that from beef lung had a pH optimum of 3.6. Their activity was not affected by thiol reagents or by Fe2+, Mn2+, or Mg2+. One isoenzyme from rabbit lung was used to immunize a goat, and one from beef lung was used to immunize a rabbit. In immunoelectrophoresis, each resulting antiserum formed a single precipitin line with its homologous enzyme. They cross-reacted with the other three isoenzymes from the same species, but not with any isoenzyme from the other species. At high concentrations, each antiserum completely inhibited the proteolytic activity of its homologous enzyme. The antiserum against rabbit lung ...
All (3-Iactarn drugs like penicillins, cephalosporins are selective inhibitors of bacterial cell wall synthesis and therefore active against growing bacteria. Mechanism of action: Initial step is the binding of the drug to specific drug receptor PBPs (Penicillin- binding proteins) on bacteria. There are 3 to 6 PBPs having different effects. At least some of which are enzymes involved in transpeptidation (cross-linking) reactions. After attachment, peptidoglycan synthesis is inhibited as final transpeptidation is blocked. Then there occurs inactivation of an inhibitor of autolytic enzyme in the cell wall. This activates the autolytic enzymes in the cell wall that results in lysis resulting in bacterial death. Organisms with defective autolysin function are inhibited but not killed by l3dactam drugs, and they are said to be tolerant ...
Dr. Akeisha Belgrave conducted research in Cell Analysis and Modeling and Biophysics at the University of Connecticut Health Center and Princeton University as a doctoral student and postdoctoral student respectively. She conducted extensive studies on the shape maintenance and motility of rod-shaped bacteria.. Dr. Belgrave is currently working with undergraduate students on their experiential projects and independent research at the university to probe aquatic biofilms towards a healthy ecosystem and also to determine the effects of bacterial cell wall turnover towards a change in antibiotic efficacies, motility, and cell shape.. ...
The lysosomes are used for the digestion of macromolecules from phagocytosis (ingestion of other dying cells or larger extracellular material), endocytosis (where receptor proteins are recycled from the cell surface), and autophagy (where old or unneeded organelles or proteins, or microbes which have invaded the cytoplasm are delivered to the lysosome). Autophagy may also lead to autophagic cell death, a form of programmed self-destruction, or autolysis, of the cell, which means that the cell is digesting itself. Other functions include digesting foreign bacteria (or other forms of waste) that invade a cell and helping repair damage to the plasma membrane by serving as a membrane patch, sealing the wound. Lysosomes also do much of the cellular digestion required to digest tails of tadpoles and to remove the web from the fingers of a 3-6 month old fetus. This process of programmed cell death is called apoptosis.[1] ...
To maintain the tissue in as lifelike a state as possible, tissue for analysis is usually placed directly into a fixative solution upon removal from the body. Fixation is normally carried out as soon as possible to prevent autolysis and to reduce possible infectivity. Several factors determine the choice of fixative for a given application. If morphological changes are known to take place rapidly after tissue collection-as with neural tissue-the speed of fixing action is extremely important.. For large tissue samples, the rate of penetration of the fixative into the sample must also be considered. If immunological detection methods are to be used, a fixative which preserves protein structure is required. For electron microscopy, fixatives which do not precipitate proteins avoid artifacts invisible to light microscopy. The price of a fixative may also be a factor. The low cost of formaldehyde fixatives is one reason for their popularity. A partial list of fixatives would include (grouped by ...
Im looking for tumors in the small and large intestines of mice. Ive been inflating the intestines with formalin immediately after removal to prevent autolysis. It also stretches the intestines making them easier to cut open and, if things work well, flushes the lumen. However, there are often tight constricted areas that do not inflate or flush. I was thinking that things would be simple if the intestines were flaccid in the first place. Does anyone have a technique to relax the intestines prior to flushing with formalin? Claude Nagamine MIT, Div. Comp. Medicine Cambridge, MA _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ...
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[169 Pages Report] Specialty Yeast Market report categorizes the global market by Species (Saccharomyces Cerevisiae, Pichia Pastoris, Kluyveromyces), Application, Type (Yeast Extracts, Yeast Autolysates, Yeast Beta-glucan, and Other Yeast Derivatives), and by Region
de Eugenio, L.I., García, P., Luengo, J.M., Sanz, J., San Román, J., García, J.L., and Prieto, M.A. (2007) Biochemical evidence that phaZ gene encodes a specific intracellular medium chain length polyhydroxyalkanoate depolymerase in Pseudomonas putida KT2442: characterization of a paradigmatic enzyme. J Biol Chem 282: 4951-4962 ...
Although the criteria used in the macroscopic evaluation of cholecystectomy specimens are similar in all the pathological departments, there may be interdepartmental differences in macroscopic sampling. Meticulous macroscopic evaluation and sampling constitute the first steps to correct histopathological diagnosis. Histopathologically, the mucosa of the gallbladder may not always be observed throughout the sample taken due to autolysis, mucosal denudation, or curling. Because the epithelium of gallbladder is quite susceptible to bile-related autolysis, prompt fixation of gallbladder samples is advisable.1 Thus, embedding of the samples must be careful and precise. In this study, the sample taken longitudinally was rolled with the swiss-roll method in almost all cases in the 2nd group. However, POM was found to be similar in both groups. This result indicates that rolling the sample with the swiss-roll method was not a solution to the problem that the mucosa could not be observed throughout ...
Inactivated yeast or semi-purified fractions of it have been used as additives for animal and fish feeds. These preparations have been proposed to encourage animal growth and health by various mechanisms, including immunomodulation, oxidative status, binding of toxins and pathogens, and interactions with gut ingredients.. Inactivated yeast or yeast autolysate contains the water-soluble and insoluble components of the yeast cell, the composition of which is primarily amino acids, peptides and carbohydrates. Yeast autolysate is manufactured by breaking down the cells using endogenous or exogenous enzymes. There have been many reports on the manufacturing processes.. At the end of yeast fermentation, yeast cells were separated by centrifugation from the spend medium and washed twice with process water and stored in large storage tanks at 4 oC. First step for the manufacturing of Inactivated Yeast is the cell inactivation, disruption and autolysis. For that purpose several methods may be ...
I believe that I may have found something truly original and worthwhile that might be done in the New World.1 Its worthwhile not just because it is novel-this is the idea of growing grapes from seeds-but because I think that it can create a real paradigm shift in how we experience wine.. Broadly speaking, the qualities that we experience in wine come from three major sources-you can almost conceive of them as radio signals of greater or lesser strength: 1) The inherent qualities of the site itself (its terroir); this is potentially the strongest signal, but it can also be quite obscured by grapegrowing and winemaking practices, drip irrigation most notably; 2) the characteristics imparted by the selection of the plant material-rootstock and scion, from the ripening properties of the vines themselves to the flavor profile of the grape varieties; 3) the overlay of winemaking technique-barrel character, diacetyl or malolactic character, lees autolysis, the qualities imparted by designer yeasts ...
Ian, Ive had much luck sith a 11.5 Kb low copy (pSC101) plasmid. I grow in TB terrific broth as described in the new Maniatis (24g YE, 12g Tryp, glycerol, PO4 buffer) with plenty of aeration (tube roller). A standard alkaline lysis followed by neutralizing with a half volume of 7.5 M ammonium acetate will do the job. When a 1.5 ml miniprep is resuspended in 50 ul, 5 ul will give a nice digest on an agorose gel. One trick you should try is to freeze your resuspended cell pellet after you have added lysozyme in the first step of your miniprep. This seems to have a marginal beneficail effect with DH5 as a host, but may be more important with other strains. I saw it in the inital report of pGB1, and it is a well know method of unleashing autolytic activity in one of my favorite organisms, Staph. aureus. J. Graham Biology and Chemistry Departments Indiana University -Bloomington Jim J. GrahaUniversity -Bloomington ...............It is necessary to be slightly underemployed if .............one is ...
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0007] One reason for the versatility of Ps. aeruginosa is that it produces a diverse battery of virulence determinants including elastase, LasA protease, alkaline protease, rhamnolipids, type IV pilus-mediated twitching motility, pyoverdin (Williams et al., 1996, Stintzi et al., 1998, Glessner et al., 1999), pyocyanin (Brint & Ohman, 1995, Reimmann et al., 1997) and the cytotoxic lectins PA-I and PA-II (Winzer et al., 2000). It is now known that many of these virulence determinants are regulated at the genetic level in a cell density-dependent manner through quorum sensing. Ps. aeruginosa possesses two well characterised quorum sensing systems, namely the las and rhl (vsm) systems which comprise of the LuxRI homologues LasRI (Gambello & Iglewski, 1991) and RhlRI (VsmRI) (Latifi et al., 1995) respectively. LasI directs the synthesis of 3-oxo-C12-HSL (Passador et al., 1993, Pearson et al., 1994) whereas RhlI directs the synthesis of C4-HSL (Winson et al., 1995). The las and the rhl systems are ...
Founded in 2001, ACMA Clinic provides professional and comprehensive health care of holistic and natural medicine.. ACMA patients come from 50 states in U.S. and countries in North America, South America, Asia, Europe, etc. Most ACMA patients first started treatments with their family doctors, specialists, local hospitals, and leading hospitals in U.S. or other countries. When those treatments could not help them, they started ACMA treatment. As a result, when they came to ACMA, they had been suffering from various severe diseases and illnesses for years, and many of them had been regarded as incurable.. However, after ACMA treatments, most patients conditions start improving for the first time in their disease history. Patients are happy to see they are recovering and returning back to normal lives.. ...
Have you ever played draughts? I think its called checkers in America. Its a board game that requires you to move pieces known as men and kings to take an opponents pieces. The pieces are often made of wood or plastic and have indentations to make stacking easier.. Its these indentations that link the game of draughts to bacteriology.. Let me introduce the pneumococcus. Formally known as Streptococcus pneumoniæ. This is a Gram-positive diplococcus that forms α-hæmolysis on blood agar. This means the colonies look green when you hold the plate up to the light.. As the bacterial cells divide and the colony grows in size, autolysis occurs in the centre of the colony forming a dimple or umbilication. Some clever bacteriologist a long time ago thought the colonies looked like draughts pieces and so in many bacteriology textbooks, youll read about how the colonies look like draughts (or checkers) pieces.. The pneumococcus can cause many infections including pneumonia, meningitis, bacteræmia, ...
A anti-bacterial is really a substance representative which is used to eliminate the quantity of worthwhile organisms on pharmaceutic surfaces to a appropriate levels. Disinfectants have many different components offering scope of activity, mode of activity, and effectiveness. Some are bacteriostatic, in which the ability with the microbe populace to reproduce is halted. In such cases, the disinfectant could cause discerning and reversible adjustments to microbial cells by getting together with nucleic acids and suppressing nutrients, or permeating to the mobile phone walls. The moment the anti-bacterial is removed from contact with microbial tissues, the enduring microbial populace could very well increase. Other disinfectants are bactericidal in that they destroy microbe cells and bring about irreparable problems through diverse elements that come with structural harm to the mobile phone, cell lysis, and autolysis, resulting in loss or coagulation of cytoplasm. The exploitation of microbe and ...
Yeast protein hydrolysate may be considered as a good source of bioactive peptides. Yeast hydrolysate was prepared by two different physical-enzymatic and autolysis treatments to identify the most active angiotensin I-converting enzyme (ACE) inhibitory and antioxidant peptides. The most active hydrolysate was obtained after sonication-trypsin hydrolysis. The hydrolysate was subjected to fractionat ...
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The issue is that some owners discontinue heartworm meds and their animals build up large loads of heartworms. A heavy dose of heartworm meds may kill those worms. Those dogs then have a significant number of dead worms in their system. Some may let go and float down stream just like a blood clot. Others will hang on and undergo autolysis, that is they disintegrate and release non-dog proteins into the bloodstream. The dogs immune system responds to those foreign proteins just like a massive infection ...
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Degradation and autolysis[edit]. Proteases, being themselves proteins, are cleaved by other protease molecules, sometimes of ... Some proteases are less active after autolysis (e.g. TEV protease) whilst others are more active (e.g. trypsinogen). ...
Effect of liver autolysis in vivo. Proc. Soc. Exp. Biol. Med., 26:304-5. With J. C. Ellis. Fatal effect of total loss of ... Liver autolysis in vivo. Arch. Surg., 20:8-16. With M. L. Montgomery, W. B. Matthews, and J. C. Ellis. Fatal effect of the ...
"Autolysis - and its effects on Champagne". Champagne Gallery. Archived from the original on 17 May 2016. Retrieved 17 May 2016 ...
... begins at the moment of death, caused by two factors: 1.) autolysis, the breaking down of tissues by the body's ... Visible changes caused by decomposition are limited during the fresh stage, although autolysis may cause blisters to appear at ... The general stages of decomposition are coupled with two stages of chemical decomposition: autolysis and putrefaction. These ...
Levin RE, Van Sickle C (1976). "Autolysis of high-GC isolates of Pseudomonas putrefaciens". Antonie van Leeuwenhoek. 42 (1-2): ... it has been observed that at least some species of bacteria with DNA of high GC-content undergo autolysis more readily, thereby ...
Heat denatures the proteolytic enzyme and prevents autolysis. Heat fixation cannot be used in the capsular stain method as heat ... One reason is to kill the tissue so that postmortem decay (autolysis and putrefaction) is prevented. Fixation preserves ... fixation is the preservation of biological tissues from decay due to autolysis or putrefaction. It terminates any ongoing ...
... this triggers autolysis, during which the yeast self-destructs. The dying yeast cells are then heated to complete their ...
Some proteases are less active after autolysis (e.g. TEV protease) whilst others are more active (e.g. trypsinogen). Proteases ...
Wireman JW, Dworkin M (February 1977). "Developmentally induced autolysis during fruiting body formation by Myxococcus xanthus ...
Martínez, Virginia; García, Pedro; García, José Luis; Prieto, María Auxiliadora (2011-07-01). "Controlled autolysis facilitates ...
Cell Wall Plant Polysaccharide-degrading Enzymes in Autolysis of Botrytis Cinerea. Science Direct, July 1980. Web. 14 Nov. 2016 ...
The autolysis of P. freudenreichii has been suggested to contribute further to the flavor of the Emmental cheese. The ... Ostlie H (1995). "Autolysis of Dairy Propionibacteria: Growth Studies, Peptidase Activities, and Proline Production". Journal ... conditions leading to the autolysis of this bacterium are not well known. P. freudenreichii was first discovered and isolated ...
They called this autolysis after Christian de Duve and Alex B. Novikoff. However Porter and Ashford wrongly interpreted their ...
Cole, Rosemary A. (1976). "Isothiocyanates, nitriles and thiocyanates as products of autolysis of glucosinolates in Cruciferae ...
Chou JS, Impens F, Gevaert K, Davies PL (July 2011). "m-Calpain activation in vitro does not require autolysis or subunit ...
Ecological Advantages of Autolysis during the Development and Dispersal of Pseudoalteromonas tunicata Biofilms. Applied and ...
Autolysis and putrefaction also play major roles in the disintegration of cells and tissues. The human body is composed of ...
... undergoes Ca2+-dependent intramolecular autolysis". FEBS Letters. 532 (3): 401-6. doi:10.1016/s0014-5793(02)03722-5. PMID ...
... significant cell autolysis had occurred, and cell definition was very poor. His brain was almost completely gone, leaving only ...
The hol-lys gene system therefore is believed to constitute a chromosomally-encoded autolysis system. The reaction probably ...
Two of these RNAs, csRNA4 and csRNA5, have been shown to affect stationary-phase autolysis. Bacterial small RNA Halfmann, A; ...
Phytoplankton can also produce DOC by autolysis during physiological stress situations e.g., nutrient limitation. Other studies ...
"Autolysis and aging of Penicillium chrysogenum cultures under carbon starvation: Chitinase production and antifungal effect of ...
... leading them to autolysis and death. Visualization of hemolysis (UK: haemolysis) of red blood cells in agar plates facilitates ...
Callister used autolysis to break down the yeast cells from waste obtained from the Carlton & United brewery. Concentrating the ...
The fermentation happens through autolysis and starts from a lactic acid enzyme in the spine of the fish. Together with ...
... autolysis (process of self digestion) and putrefaction (process caused by bacteria found within the body). More advanced ...
Sieradzki K, Tomasz A (April 1997). "Inhibition of cell wall turnover and autolysis by vancomycin in a highly vancomycin- ...
This autolysis of the cell provides an available nitrogen source for the still-fermenting and viable yeast cells. However, this ... a number of changes can impact the wine due to both the autolysis (or self-metabolize) of the dead yeast cells as well as the ... autolysis can also release sulfur-link compounds (such as the breakdown of amino acid cysteine) which can combine with other ... benefits associated with lees contact deals with the influence on the wine of the mannoproteins released during the autolysis ...
While the wine is being aged, the autolysis of yeast occurs which gives the wine the sparkling component. Straw wine - Straw ...
Source for information on autolysis: A Dictionary of Zoology dictionary. ... autolysis The destruction of a cell or some of its components through the action of its own hydrolytic enzymes (see HYDROLYSIS ... autolysis The destruction of a cell or some of its components through the action of its own hydrolytic enzymes (see HYDROLYSIS ... autolysis A Dictionary of Zoology © A Dictionary of Zoology 1999, originally published by Oxford University Press 1999. ...
Autolysis may refer to: Autolysis (biology), the destruction (or lysis) of a cell by its own enzymes Autocatalysis, in ... in the production of commercial extract This disambiguation page lists articles associated with the title Autolysis. If an ... or catalyzes its own transformation into another compound Autolysis (alcohol fermentation), the complex chemical reactions that ...
In beer brewing, autolysis causes undesired off-flavors. Autolysis in winemaking is often undesirable, but in the case of the ... autolysis in itself is not an active process. In other words, though autolysis resembles the active process of digestion of ... In biology, autolysis, more commonly known as self-digestion, refers to the destruction of a cell through the action of its own ... In terms of autolysis, peroxisomes provide catabolic potential for fatty acids and reactive oxygen species, which are released ...
autolysis synonyms, autolysis pronunciation, autolysis translation, English dictionary definition of autolysis. n. The breaking ... Autolysis - definition of autolysis by The Free Dictionary https://www.thefreedictionary.com/autolysis ... autolysis. Also found in: Thesaurus, Medical, Encyclopedia, Wikipedia. au·tol·y·sis. (ô-tŏl′ĭ-sĭs). n.. The breaking down of ... autolysis. (ɔːˈtɒlɪsɪs) n. (Biochemistry) the destruction of cells and tissues of an organism by enzymes produced by the cells ...
... The trypsin used was side-chain protected porcine trypsin from Promega. The enzyme was derivatized ... The data suggest that it underwent some autolysis prior to and/or during the derivatization. The peptides in bold were ... by reductive methylation (Lys side-chains) to prevent autolysis. ...
"Autolysis" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Targeting the autolysis loop of urokinase-type plasminogen activator with conformation-specific monoclonal antibodies. Biochem ... This graph shows the total number of publications written about "Autolysis" by people in Harvard Catalyst Profiles by year, and ... Increased pathogenicity of pneumococcal serotype 1 is driven by rapid autolysis and release of pneumolysin. Nat Commun. 2020 04 ...
The rate of whole cell autolysis of S. aureus was growth phase dependent and a maximum rate was observed in early stationary ... The effect of physiological conditions on autolysis and autolytic activity in various strains of Staphylococcus aureus was ... The rate of autolysis of cultures grown at 30 degrees C was higher than cultures grown at 37 or 43 degrees C. Thus, the rate of ... Effect of physiological conditions on the autolysis of Staphylococcus aureus strains Antonie Van Leeuwenhoek. 1994;65(1):71-8. ...
... and controlled autolysis of E. coli for protein expression and purification. ... XJb (DE3) Autolysis Glycerol Stock, 1 ml 500x L-Arabinose. 1 ml. $109.00. - + ... Autolysis. XJb lysis efficiency is 10-20 % lower than XJa. For optimal lysis, more care needs to be taken when selecting the ... The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). It can be ...
Martínez, V., García, P., García, J. L. and Prieto, M. A. (2011), Controlled autolysis facilitates the polyhydroxyalkanoate ...
The potential of the application of PEF to induce accelerate autolysis of a commercial strain of Sacharomyces cerevisiae for ... The potential of the application of PEF to induce accelerate autolysis of a commercial strain of Sacharomyces cerevisiae for ... Mannoproteins are released from the yeast cell wall during yeast autolysis. Autolysis is a phenomenon that begins with the ... The autolysis of yeast in wine is a very slow process lasting from a few months to years. Therefore accelerating this process ...
Lancker, J. L. van, Holtzer, R. L.: The fate of nuclei, DNA, and DNase in the course of autolysis. Lab. Invest.12, 102-105 ( ... Nunley, W. C., Schuit, K. E., Dickie, M. W., Kinlaw, J. B.: Delayed,in vivo hepatic postmortem autolysis. Virchows Arch. Abt. B ... Delayed,in vivo hepatic postmortem autolysis. II. Morphological changes in reticulo-endothelial cells ... The release of acid phosphatase and β-glucuronidase from cytoplasmic granules in the early course of autolysis. Amer. J. Path. ...
Calpain 2 Activation, Autolysis, and Subunit Dissociation  Chou, Jordan (2010-10-25) ... Browsing Queens Graduate Theses and Dissertations by Subject "Autolysis". 0-9. A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P ...
Identification and molecular characterization of a putative regulatory locus that affects autolysis in Staphylococcus aureus.. ... The lytS mutant also exhibited increased autolysis and an altered level of murein hydrolase activity produced compared with the ... Identification and molecular characterization of a putative regulatory locus that affects autolysis in Staphylococcus aureus. ... Identification and molecular characterization of a putative regulatory locus that affects autolysis in Staphylococcus aureus. ...
The vir locus affects the response of Bordetella pertussis to antibiotics: phenotypic tolerance and control of autolysis.. ...
Autolysis of methicillin-resistant and -susceptible Staphylococcus aureus.. J E Gustafson, B Berger-Bächi, A Strässle, B J ... Autolysis of methicillin-resistant and -susceptible Staphylococcus aureus.. J E Gustafson, B Berger-Bächi, A Strässle, B J ... Autolysis of methicillin-resistant and -susceptible Staphylococcus aureus.. J E Gustafson, B Berger-Bächi, A Strässle, B J ... Autolysis of methicillin-resistant and -susceptible Staphylococcus aureus. Message Subject (Your Name) has forwarded a page to ...
He had no doubt that autolysis causes the yeastiness in champagne, adding that autolysis is expressed quite differently by ... In biology autolysis refers to the destruction of a cell through the action of its own enzymes. The term "autolyse" was coined ... Champagne is bready from autolysis. A guest article by Estelle Platini. Or isnt it? ... If so-called yeast autolysis character comes mainly from pinot noir why do sparkling wine makers waste their time and money ...
Autolysis of H. pylori is an important phenomenon to recognize since it likely exerts significant effects on the behavior of H ... Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis.. S H Phadnis ... Taken together, these results strongly suggest that H. pylori cells undergo spontaneous autolysis during culture and that ... Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis. ...
Isolation and characterization of a possible native cucumisin from developing melon fruits and its limited autolysis to ... Isolation and characterization of a possible native cucumisin from developing melon fruits and its limited autolysis to ... and that cucumisin is a product derived by the limited autolysis of the 67-kD proteinase. The 67-kD proteinase was more stable ... it was split into a 54-kD proteinase and a 14-kD polypeptide by limited autolysis without any loss of the caseinolytic activity ...
Effects of temperature, NaCl, and methicillin on penicillin-binding proteins, growth, peptidoglycan synthesis, and autolysis in ... Effects of temperature, NaCl, and methicillin on penicillin-binding proteins, growth, peptidoglycan synthesis, and autolysis in ... Effects of temperature, NaCl, and methicillin on penicillin-binding proteins, growth, peptidoglycan synthesis, and autolysis in ... Effects of temperature, NaCl, and methicillin on penicillin-binding proteins, growth, peptidoglycan synthesis, and autolysis in ...
The activity of complex III (CoQH2-c reductase) decreased at a more gradual rate during ischemia or autolysis, and its rate of ... The activity of complex V (oligomycin-sensitive ATPase) decreased most rapidly (by 40% in only 5 min of autolysis) but nearly ... but largely only after 20 min of ischemia or autolysis. At 20 min and beyond, the decrease in the activity of complex I ... the usefulness of simple tissue autolysis as an analogue of in situ myocardial ischemia. ...
Moreover, we observed that Cwp19 is involved in cell autolysis and that a mutant exhibited delayed autolysis in stationary ... Wild-type cell autolysis is correlated to strong alterations of cell wall thickness and integrity and to release of cytoplasmic ... Cwp19 Is a Novel Lytic Transglycosylase Involved in Stationary-Phase Autolysis Resulting in Toxin Release in. ... In contrast, Cwp19 did not induce autolysis or toxin release when cells were grown in tryptone-yeast extract (TY) medium. These ...
Contributions of Basic Amino Acids in the Autolysis Loop of Factor XIa to Serpin Specificity † ...
... - [ô täl′ə sis] n. [ AUTO + LYSIS] the destruction of cells or tissues by their own enzymes, as after death or in ... Autolysis - The enzymatic digestion of cells by enzymes present within them. The cells most susceptible to autolysis tend to be ... autolysis - autolytic /awt l it ik/, adj. /aw tol euh sis/, n. Biochem. the breakdown of plant or animal tissue by the action ... autolysis - the breakdown of proteins, fats and other body components of fish after death caused by the action of enzymes. The ...
Significant correlations were found between different calpain autolysis products and intensity of intact desmin and moisture ... and y-calpain and regulated the rate of y-calpain autolysis. In the third experiment, the hypothesis that the muscle fiber type ... protein nitrosylation and calpain autolysis affect the development of fresh meat quality. The first experiment was designed to ... Involvement of protein degradation, calpain autolysis and protein nitrosylation in fresh meat quality during early postmortem ...
K. Autolysis:. Adequate animal husbandry practices should be employed so that tissues and/or organs are not lost to a study ... Necropsy should be performed soon after an animal is sacrificed or found dead, so that loss of tissues due to autolysis is ... the animal should be refrigerated at a temperature that is low enough to prevent autolysis but not so low as to cause cell ...
... loss of animals and tissues or organs in a study because of autolysis. Autolysis in excess of this standard may be a cause to ... Necropsy should be performed soon after an animal is sacrificed or found dead, so that loss of tissues due to autolysis is ... N. Autolysis:. Adequate animal husbandry practices should result in considerably less than 10 % ... the animal should be refrigerated at a temperature that is low enough to prevent autolysis (i.e., between 4oC and 8oC), but not ...
Degradation and autolysis[edit]. Proteases, being themselves proteins, are cleaved by other protease molecules, sometimes of ... Some proteases are less active after autolysis (e.g. TEV protease) whilst others are more active (e.g. trypsinogen). ...
Evidence for Yeast Autophagy during Simulation of Sparkling Wine Aging: A Reappraisal of the Mechanism of Yeast Autolysis in ...
Lipids of rat heart were stable enough at early periods of the autolysis. At the same time, the most labile fraction included ... Reference: Gribanov G.A., Sergeev S.A., Lipids of rat myocardium in autolysis, Voprosy meditsinskoi khimii, 1983, vol: 29(4), ... The main pathways of lipid degradation within the first 24 hrs of autolysis operated via hydrolysis of phospholipids, neutral ... composition were studied by means of micro-thin-layer chromatography technique in rat myocardium during postmortal autolysis. ...
... Academic Article * View record in Web of Science ® ... Autolysis of eel AChE may give rise to a neuropeptide processing enzyme. ... indicate that AChEs trypsin-like activity is an integral component of the molecule and that it is activated by autolysis. ...
  • The addition of NaCl to the growth medium enhanced the rate of autolysis with the highest rate being displayed by cultures grown in 1.5 M NaCl. (nih.gov)
  • The rate of autolysis of cultures grown at 30 degrees C was higher than cultures grown at 37 or 43 degrees C. Thus, the rate of autolysis seems to be independent of the bacterial growth rate. (nih.gov)
  • Cultures grown in slightly acidic conditions showed a faster rate of autolysis compared to cultures grown under alkaline conditions. (nih.gov)
  • SDS-polyacrylamide gel containing 0.2% crude cell-wall of S. aureus did not show any obvious correlation with the appearance of any particular lytic band in the zymogram to autolytic activity or rate of autolysis of cultures grown under various environmental conditions. (nih.gov)
  • A nonhemolytic phenotype, mutations in the accessory gene regulator, and lysogeny (phages phi 11, phi 12, phi 13) had no obvious effect either on the rate of autolysis or on the pattern of lytic bands in the zymograms. (nih.gov)
  • These data suggest that the lytS and lytR gene products control the rate of autolysis in S. aureus by affecting the intrinsic murein hydrolase activity associated with the cell. (asm.org)
  • These results suggest that the presence of the methicillin resistance region, mec, either directly or indirectly through an interaction with other host genes, confers a faster rate of autolysis on strains. (asm.org)
  • Various auxilliary genes are known to affect methicillin resistance expression, and one of these genes, femA, was necessary for the expression of this faster rate of autolysis. (asm.org)
  • Our observations suggest that methicillin resistance expression is associated with an enhanced rate of autolysis, in heterogeneous resistant strains at least. (asm.org)
  • Identification and molecular characterization of a putative regulatory locus that affects autolysis in Staphylococcus aureus. (asm.org)
  • The effect of physiological conditions on autolysis and autolytic activity in various strains of Staphylococcus aureus was determined. (nih.gov)
  • Autolysis of methicillin-resistant and -susceptible Staphylococcus aureus. (asm.org)
  • The autolysis of bacterial cells as part of a regulated death program seems to be an important source for eDNA in diverse species, including Staphylococcus aureus ( 4 , 36 , 37 ), Staphylococcus epidermidis ( 35 ), Enterococcus faecalis ( 44 ), and Pseudomonas aeruginosa ( 1 ). (pubmedcentralcanada.ca)
  • We have previously identified mgrA ( rat ) as a regulator of autolysis in Staphylococcus aureus . (dartmouth.edu)
  • autolysis - Spontaneous lysis (rupture) of cells or organelles produced by the release of internal hydrolyic enzymes. (enacademic.com)
  • The lytS mutant also exhibited increased autolysis and an altered level of murein hydrolase activity produced compared with the parental strain, NCTC 8325-4. (asm.org)
  • Faster rates of autolysis were noted in two heterogeneous methicillin-resistant transductants than in their methicillin-susceptible parental recipients, in a heterogeneous resistant strain than in a susceptible derivative created by chemical mutagenesis, and in a homogeneous resistant strain than in a derivative that had decreased methicillin resistance and was created by transposon Tn551 mutagenesis. (asm.org)
  • The rates of autolysis and proteolysis observed in vitro were highly strain-dependent. (scirp.org)
  • The vir locus affects the response of Bordetella pertussis to antibiotics: phenotypic tolerance and control of autolysis. (nih.gov)
  • The cells most susceptible to autolysis tend to be dying or dead cells. (enacademic.com)
  • These stained tissues may require a high level of oxygen, making them more susceptible to autolysis. (elsevier.com)
  • The objective was to determine the extent to which protein modifications including protein degradation, protein nitrosylation and calpain autolysis affect the development of fresh meat quality. (iastate.edu)
  • Significant correlations were found between different calpain autolysis products and intensity of intact desmin and moisture loss. (iastate.edu)
  • The combination of S-nitrosoglutathione and calcium affected the activity of m- and y-calpain and regulated the rate of y-calpain autolysis. (iastate.edu)
  • Interestingly, GPR30 agonist G1 triggered calpain 1 autolysis but not calpain 2, whereas ER agonist diethylstilbestrol caused no apparent calpain autolysis. (osti.gov)
  • The enzyme was derivatized by reductive methylation (Lys side-chains) to prevent autolysis. (ucsf.edu)
  • To prevent autolysis and putrefaction. (slideshare.net)
  • Alterations in lipid composition were studied by means of micro-thin-layer chromatography technique in rat myocardium during postmortal autolysis. (msk.ru)
  • The data obtained suggest that separate types of sRNA are degraded selectively at early steps of postmortal autolysis. (msk.ru)
  • however, pathologic studies were limited by the advanced state of postmortem autolysis. (cdc.gov)
  • Mannoproteins are released from the yeast cell wall during yeast autolysis. (frontiersin.org)
  • The autolysis of yeast in wine is a very slow process lasting from a few months to years. (frontiersin.org)
  • For making sparkling wine, autolysis involves killing the yeast and encouraging the breakdown of the cells by enzymes. (frenchscout.com)
  • Well-known amateur sparkling winemaker, MF (those five years spent as sous-remueur - translation: riddler - at Bolli were not wasted) reckons that the bready character does in fact come from yeast autolysis - rather than aged pinot noir or pinot meunier. (frenchscout.com)
  • If so-called yeast autolysis character comes mainly from pinot noir why do sparkling wine makers waste their time and money leaving fizz in contact with yeast sediment for years, when they could just as easily add more pinot noir to the brew? (frenchscout.com)
  • In contrast, Cwp19 did not induce autolysis or toxin release when cells were grown in tryptone-yeast extract (TY) medium. (pasteur.fr)
  • And yeast performance by the fermentation process conditions and the appearance of environmental factors such as the impact of changes in the inevitable emergence of yeast aging, death and autolysis, how to reduce yeast autolysis, extend the life of yeast, beer is to ensure the quality of the fundamental basis for stability. (zrbrewing.com)
  • Beer fermentation process Yeast autolysis is inevitable, but the degree of autolysis and autolysis speed is different. (zrbrewing.com)
  • Our aim is not to prevent yeast autolysis, but to control the degree of yeast autolysis, delay the process of yeast aging. (zrbrewing.com)
  • Release of DNA without detectable autolysis is suggested to be an adaptation to the competitive oral biofilm environment, where autolysis could create open spaces for competitors to invade. (pubmedcentralcanada.ca)
  • There is autolysis of bacterial cells and release of intracellular contents including DNA (Palmen and Hellingwerf, 1995, Palmen and Hellingwerf, 1997). (thefreedictionary.com)
  • This has the effect of inducing rapid cell death (autolysis) in the majority of bacterial cells, and preventing virulence or restoring a benign state in surviving cells. (patentsencyclopedia.com)
  • Several lines of evidence indicate that AChE's trypsin-like activity is an integral component of the molecule and that it is activated by autolysis. (edu.au)
  • In biology, autolysis, more commonly known as self-digestion, refers to the destruction of a cell through the action of its own enzymes. (wikipedia.org)
  • autolysis - [ɔ: tɒlɪsɪs] noun Biology the destruction of cells or tissues by their own enzymes. (enacademic.com)
  • In other words, though autolysis resembles the active process of digestion of nutrients by live cells, the dead cells are not actively digesting themselves as is often claimed, and as the synonym self-digestion suggests. (wikipedia.org)
  • Oxidation inhibits substrate proteolysis by calpain I but not autolysis. (uab.edu)
  • In this study, the effects of oxidation on calpain I autolysis and calpain-mediated proteolysis were examined. (uab.edu)
  • We demonstrate here that both 17-beta-estradiol (E2) and ICI enhance cell adhesion to matrigel in MCF-7 breast cancer cells, with increased autolysis of calpain 1 (large subunit) and proteolysis of focal adhesion kinase (FAK), indicating calpain activation. (osti.gov)
  • The autolysis and proteolysis are important features in the strains of L. lactis used in the manufacture of cheese. (scirp.org)
  • The potential of the application of pulsed electric fields (PEF) to induce accelerate autolysis of a commercial strain of Saccharomyces cerevisiae for winemaking use was evaluated. (frontiersin.org)
  • By binding to such molecules, the receptors can be used to modulate the extra-cellular concentrations of molecules involved in environment-sensing and virulence in Pseudomonas aeruginosa, and in so doing can induce a process of rapid cell death (autolysis) within bacterial populations. (patentsencyclopedia.com)
  • Autolysis is uncommon in living adult organisms and usually occurs in necrotic tissue as enzymes act on components of the cell that would not normally serve as substrates. (wikipedia.org)
  • 1.Provide water to the scab to help wound debridement autolysis.The necrotic tissue can wrapped separated and removed. (medicalwounddressing.com)
  • In addition, autopsy may be impaired by tissue autolysis. (springer.com)
  • The rate of whole cell autolysis of S. aureus was growth phase dependent and a maximum rate was observed in early stationary phase cultures. (nih.gov)
  • Changes confirmed with ultrastructural analyses included nuclear chromatin aggregation and margination, marked reduction of rough endoplasmic reticulum and Golgi complexes, vascular degenerations, and necrosis and autolysis of tumor cells with dark cells and/or naked nuclear cells. (thefreedictionary.com)
  • TUNEL staining detects not only apoptosis but also late necrosis or autolysis because it detects DNA breaks. (elsevier.com)
  • In Volvariella volvacea, a species of edible mushroom, cryogenic autolysis is a typical part of abnormal metabolism. (nebraska.edu)
  • No more profound change in metabolism is possible than that produced by fasting and the change is of a character best suited to bring about the autolysis of a tumor, malignant or otherwise. (chestofbooks.com)
  • Modification of carcinogen metabolism by indolylic autolysis products of Brassica oleraceae. (webmd.com)
  • autolysis The destruction of a cell or some of its components through the action of its own hydrolytic enzymes (see HYDROLYSIS ). (encyclopedia.com)
  • In terms of autolysis, peroxisomes provide catabolic potential for fatty acids and reactive oxygen species, which are released into the cytosol as the peroxisomal membrane is damaged by water retention and digestion by other catabolic enzymes. (wikipedia.org)
  • During autolysis enzymes glucanase and proteinase degrade the cell wall and, as result, the cell wall becomes porous and different compounds such as mannoproteins are released into the surrounding medium ( Alexandre and Guilloux-Benatier, 2006 ). (frontiersin.org)
  • Autolysis - The enzymatic digestion of cells by enzymes present within them. (enacademic.com)
  • autolysis - the breakdown of proteins, fats and other body components of fish after death caused by the action of enzymes. (enacademic.com)
  • autolysis - noun a) The destruction of an organisms cells by enzymes produced by the organism itself. (enacademic.com)
  • J. Ramírez-Nunez, R. Romero-Medrano, G. V. NevárezMoorillon and N. Gutiérrez-Méndez, "Effect of pH and Salt Gradient on the Autolysis of Lactococcus lactis Strains," Brazilian Journal of Microbiology, Vol. 42, No. 4, 2011, pp. 1495-1499. (scirp.org)
  • Autolysis may occur in inner ear structures during fixation. (elsevier.com)
  • Taken together, these results strongly suggest that H. pylori cells undergo spontaneous autolysis during culture and that urease and HspB become surface associated only concomitant with bacterial autolysis. (asm.org)
  • Glycolysis has a lower ATP yield than oxidative phosphorylation and generates acidic byproducts that decrease the pH of the cell, which enables many of the enzymatic processes involved in autolysis. (wikipedia.org)
  • Moreover, we observed that Cwp19 is involved in cell autolysis and that a mutant exhibited delayed autolysis in stationary phase compared to the wild type when bacteria were grown in brain heart infusion (BHI) medium. (pasteur.fr)
  • Wild-type cell autolysis is correlated to strong alterations of cell wall thickness and integrity and to release of cytoplasmic material. (pasteur.fr)
  • Thus, Cwp19 is an important surface protein involved in autolysis of vegetative cells of that mediates the release of the toxins from the cell cytosol in response to specific environment conditions. (pasteur.fr)
  • Surprisingly, S. sanguinis and S. gordonii cell integrity appears unaffected by conditions that cause autolysis in other eDNA-producing bacteria. (pubmedcentralcanada.ca)
  • Differentiation of tracheary elements (TEs) is finalized by programmed cell death (PCD) and autolysis. (ebscohost.com)
  • This review integrates TE differentiation, PCD, and autolysis in a biological and evolutionary context.Tracheary element (TE) differentiation represents a unique system to study plant developmental programmed cell death (PCD). (ebscohost.com)
  • TE PCD is tightly followed by autolysis of the protoplast and partial hydrolysis of the primary cell walls. (ebscohost.com)
  • This review integrates TE differentiation, programmed cell death (PCD), and autolysis in a biological and evolutionary context. (ebscohost.com)
  • The collective evidence from the evolutionary and molecular studies suggests that TE differentiation consists primarily of a programme for cell death and autolysis under the direct control of the transcriptional master switches VASCULAR NAC DOMAIN 6 (VND6) and VND7. (ebscohost.com)
  • Therefore, TE differentiation involves, in addition to PCD, a programmed autolysis which is initiated before cell death and executed post-mortem. (ebscohost.com)
  • Therefore, the importance of distinguishing between the cell death programme per se and autolysis in all plant PCD research and of careful description of the morphological, biochemical, and molecular sequences in each of these processes, is advocated. (ebscohost.com)
  • Differentiation of tracheary elements (TEs), characterized by formation of a visible secondary cell wall and autolysis, has been regarded as a model system for cytodifferentiation in plants. (ebscohost.com)
  • This can avoid the cell autolysis [ 16 ]. (hindawi.com)
  • Autolysis" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • Lipids of rat heart were stable enough at early periods of the autolysis. (msk.ru)
  • The main pathways of lipid degradation within the first 24 hrs of autolysis operated via hydrolysis of phospholipids, neutral lipids as well as via reactions of transacetylation and biotransformation (methylation etc). (msk.ru)
  • Autolytic conversion of the 80-kDa subunit of calpain I was not detected until free calcium concentration was greater than 40 microM, whereas autolysis of the 30-kDa subunit did not occur until the free calcium concentration was greater than 100 microM. (uab.edu)
  • Autolysis produces an acidic, anaerobic, nutrient-rich environment that nurtures the activity of invasive and opportunistic microorganisms in a process known as putrefaction. (wikipedia.org)
  • Once these rupture, the nutrient-rich fluids present within the blisters then fuel the onset of the second major phase of decomposition, called putrefaction, which is the process where microbiological organisms (in the body, on the body and in the environment) feed on the nutrient-rich fluids produced during autolysis. (scientificamerican.com)
  • Their excellent survival during autolysis in vitro and ischemia in vivo is consistent with this. (dict.cn)
  • Autolysis during In Vitro Tracheary Element Differentiation: Formation and Location of the Perforation. (ebscohost.com)
  • In vitro , we demonstrate that the presence of Spn1 correlated with a defect in LytA-mediated autolysis. (asm.org)
  • Autolysis is a phenomenon that begins with the disorganization of membranous systems (cytoplasmic membrane and other organelle membranes) caused by cell's death. (frontiersin.org)
  • Lancker, J. L. van, Holtzer, R. L.: The release of acid phosphatase and β-glucuronidase from cytoplasmic granules in the early course of autolysis. (springer.com)
  • Notably, myrtenol treated cells were deficient in extracellular DNA (eDNA) mediated autoaggregation as eDNA releasing autolysis was impaired by myrtenol. (frontiersin.org)
  • Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis. (asm.org)
  • Pathogenesis of is mainly attributed to the production and secretion of toxins A and B. Unlike most clostridial toxins, toxins A and B have no signal peptide, and they are therefore secreted by unusual mechanisms involving the holin-like TcdE protein and/or autolysis. (pasteur.fr)
  • Note: Autolysis occurs to tissues of higher animals when they are detached from the organism. (enacademic.com)
  • Autolysis and putrefaction are the main processes responsible for the decomposition of remains. (wikipedia.org)
  • With more load cycles, autolysis processes would hae impaired the results. (dict.cn)
  • It has recently become clear that TE PCD and autolysis are separate processes with separate molecular regulation. (ebscohost.com)
  • Increased pathogenicity of pneumococcal serotype 1 is driven by rapid autolysis and release of pneumolysin. (harvard.edu)
  • Early rigor mortis and rapid autolysis indicate the need for prompt necropsy examinations. (dict.cn)
  • In fevers we have rapid autolysis in many tissues of the body and much reparative work going on, but we cannot develop a fever at will. (chestofbooks.com)
  • The pH and the NaCl concentration in the media affected significantly the autolysis of L. lactis . (scirp.org)
  • When the purified 67-kD enzyme was incubated at 50.degree.C and pH 7.2, it was split into a 54-kD proteinase and a 14-kD polypeptide by limited autolysis without any loss of the caseinolytic activity. (eurekamag.com)
  • Thus, it was concluded that the purified 67-kD enzyme is a native form of cucumisin, and that cucumisin is a product derived by the limited autolysis of the 67-kD proteinase. (eurekamag.com)
  • autolysis in itself is not an active process. (wikipedia.org)
  • This process is known as autolysis. (slideshare.net)
  • Pathologic process and autolysis were assessed in all samples. (wiley.com)
  • Physiologists have studied the process of autolysis , although they have suggested no practical use that may be made of it save that of employing it to reduce weight. (chestofbooks.com)
  • The process of autolysis may be put to great practical use and may be made to serve in getting rid of tumors and other growths. (chestofbooks.com)
  • It now remains for physiologists to learn that by means of rigidly controlled autolysis, the body is able to digest tumors and utilize the proteins and other food elements contained in them to nourish its vital tissues. (chestofbooks.com)
  • Calpain I was found to have proteolytic activity at free calcium concentrations below that required for autolysis. (uab.edu)
  • Interestingly, whereas oxidation did not inhibit autolytic conversion, the presence of high substrate concentrations did result in a significant reduction of autolysis without altering calpain proteolytic activity. (uab.edu)
  • In conclusion, autolysis of pancreas secondary to intraacinar enzyme activation is the most accepted theory in the pathogenesis of pancreatitis. (thefreedictionary.com)
  • He was once interviewed on the cause of the bready, yeasty character found in champagne and other sparkling wine: "most of that is just bottle-developed pinot noir character (rather) than autolysis," said Steve. (frenchscout.com)
  • He had no doubt that autolysis causes the yeastiness in champagne, adding that autolysis is expressed quite differently by different grape varieties. (frenchscout.com)
  • Autolytica autolysis Metallica Champagne wine 5 panel cap - Black. (etsy.com)
  • Autolytica autolysis Metallica Champagne Wine Sweatshirt - Black. (etsy.com)
  • two of them were distinctly decreased after 24 hrs autolysis but the third one was maintained almost without alterations. (msk.ru)