The spontaneous disintegration of tissues or cells by the action of their own autogenous enzymes.
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
An autolytic enzyme bound to the surface of bacterial cell walls. It catalyzes the hydrolysis of the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell wall glycopeptides, particularly peptidoglycan. EC 3.5.1.28.
Cysteine proteinase found in many tissues. Hydrolyzes a variety of endogenous proteins including NEUROPEPTIDES; CYTOSKELETAL PROTEINS; proteins from SMOOTH MUSCLE; CARDIAC MUSCLE; liver; platelets; and erythrocytes. Two subclasses having high and low calcium sensitivity are known. Removes Z-discs and M-lines from myofibrils. Activates phosphorylase kinase and cyclic nucleotide-independent protein kinase. This enzyme was formerly listed as EC 3.4.22.4.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
The inactive proenzyme of trypsin secreted by the pancreas, activated in the duodenum via cleavage by enteropeptidase. (Stedman, 25th ed)
Proteins found in any species of bacterium.
Bacterial polysaccharides that are rich in phosphodiester linkages. They are the major components of the cell walls and membranes of many bacteria.
Physiological changes that occur in bodies after death.
One of the CEPHALOSPORINS that has a broad spectrum of activity against both gram-positive and gram-negative microorganisms.
A specialized proteolytic enzyme secreted by intestinal cells. It converts TRYPSINOGEN into its active form TRYPSIN by removing the N-terminal peptide. EC 3.4.21.9.
Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
Nonionic surfactant mixtures varying in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups. They are used as detergents, emulsifiers, wetting agents, defoaming agents, etc. Octoxynol-9, the compound with 9 repeating ethoxy groups, is a spermatocide.
A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
A species of gram-positive, coccoid bacteria commonly isolated from clinical specimens and the human intestinal tract. Most strains are nonhemolytic.
The predominant milk-clotting enzyme from the true stomach or abomasum of the suckling calf. It is secreted as an inactive precursor called prorennin and converted in the acid environment of the stomach to the active enzyme. EC 3.4.23.4.
A mixture of related phosphoproteins occurring in milk and cheese. The group is characterized as one of the most nutritive milk proteins, containing all of the common amino acids and rich in the essential ones.
Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed)
A 25-kDa peptidase produced by Staphylococcus simulans which cleaves a glycine-glcyine bond unique to an inter-peptide cross-bridge of the STAPHYLOCOCCUS AUREUS cell wall. EC 3.4.24.75.
A cephalosporin antibiotic.
A group of antibiotics that contain 6-aminopenicillanic acid with a side chain attached to the 6-amino group. The penicillin nucleus is the chief structural requirement for biological activity. The side-chain structure determines many of the antibacterial and pharmacological characteristics. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1065)
Exotoxins produced by certain strains of streptococci, particularly those of group A (STREPTOCOCCUS PYOGENES), that cause HEMOLYSIS.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
Antibiotic substance produced by Streptomyces garyphalus.
Hospitals controlled by agencies and departments of the state government.
The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins.
Special hospitals which provide care to the mentally ill patient.
Acute illnesses, usually affecting the GASTROINTESTINAL TRACT, brought on by consuming contaminated food or beverages. Most of these diseases are infectious, caused by a variety of bacteria, viruses, or parasites that can be foodborne. Sometimes the diseases are caused by harmful toxins from the microbes or other chemicals present in the food. Especially in the latter case, the condition is often called food poisoning.
Death resulting from the presence of a disease in an individual, as shown by a single case report or a limited number of patients. This should be differentiated from DEATH, the physiological cessation of life and from MORTALITY, an epidemiological or statistical concept.
A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.
An array of tests used to determine the toxicity of a substance to living systems. These include tests on clinical drugs, foods, and environmental pollutants.
The highest dosage administered that does not produce toxic effects.
Experiments designed to determine the potential toxic effects of a long-term exposure to a chemical or chemicals.
Experiments designed to determine the potential toxic effects of one-time, short-term exposure to a chemical or chemicals.
Laws and regulations concerned with industrial processing and marketing of foods.
Components of the usual diet that may provide health benefits beyond basic nutrients. Examples of functional foods include soy, nuts, chocolate, and cranberries (From NCCAM Backgrounder, March 2004, p3).
Experiments designed to determine the potential toxic effects of mid-term (a few months) exposure to a chemical or chemicals.

Autolysosomal membrane-associated betaine homocysteine methyltransferase. Limited degradation fragment of a sequestered cytosolic enzyme monitoring autophagy. (1/238)

We compared the membrane proteins of autolysosomes isolated from leupeptin-administered rat liver with those of lysosomes. In addition to many polypeptides common to the two membranes, the autolysosomal membranes were found to be more enriched in endoplasmic reticulum lumenal proteins (protein-disulfide isomerase, calreticulin, ER60, BiP) and endosome/Golgi markers (cation-independent mannose 6-phosphate receptor, transferrin receptor, Golgi 58-kDa protein) than lysosomal membranes. The autolysosomal membrane proteins include three polypeptides (44, 35, and 32 kDa) whose amino-terminal sequences have not yet been reported. Combining immunoblotting and reverse transcriptase-polymerase chain reaction analyses, we identified the 44-kDa peptide as the intact subunit of betaine homocysteine methyltransferase and the 35- and 32-kDa peptides as two proteolytic fragments. Pronase digestion of autolysosomes revealed that the 44-kDa and 32-kDa peptides are present in the lumen, whereas the 35-kDa peptide is not. In primary hepatocyte cultures, the starvation-induced accumulation of the 32-kDa peptide occurs in the presence of E64d, showing that the 32-kDa peptide is formed from the sequestered 44-kDa peptide during autophagy. The accumulation is induced by rapamycin but completely inhibited by wortmannin, 3-methyladenine, and bafilomycin. Thus, detection of the 32-kDa peptide by immunoblotting can be used as a streamlined assay for monitoring autophagy.  (+info)

Developmental aspects of secondary palate formation. (2/238)

Research on development of the secondary palate has, in the past, dealt primarily with morphological aspects of shelf elevation and fusion. The many factors thought to be involved in palatal elevation, such as fetal neuromuscular activity and growth of the cranial base and mandible, as well as production of extracellular matrix and contractile elements in the palate, are mostly based on gross, light microscopic, morphometric or histochemical observations. Recently, more biochemical procedures have been utilized to described palatal shelf elevation. Although these studies strongly suggest that palatal extracellular matrix plays a major role in shelf movement, interpretation of these data remains difficult owing to the complexity of tissue interactions involved in craniofacial development. Shelf elevation does not appear to involve a single motive factor, but rather a coordinated interaction of all of the abovementioned developmental events. Further analysis of mechanisms of shelf elevation requires development of new, and refinement of existing, in vitro procedures. A system that enables one to examine shelf elevation in vitro would allow more meaningful analysis of the relative importance of the various components in shelf movement. Much more is known about fusion of the palatal shelves, owing in large part to in vitro studies. Fusion of the apposing shelves, both in vivo and in vitro, is dependent upon adhesion and cell dealth of the midline epithelial cells. Adhesion betweeen apposing epithelial surfaces appears to involve epithelial cell surface macromolecules. Further analysis of palatal epithelial adhesion should be directed towards characterization of those cell surface components responsible for this adhesive interaction. Midline epithelial cells cease DNA synthesis 24-36 h before shelf elevation and contact, become active in the synthesis of cell surface glycoproteins, and subsequently manifest morphological signs of necrosis. Death of the midline epithelial cells is thought to involve a programmed, lysosomal-mediated autolysis...  (+info)

Suppression of the lytic and bactericidal effects of cell wallinhibitory antibiotics. (3/238)

The bacteriolytic effect of beta-lactam antibiotics on Bacillus subtilis and on Streptococcus pneumoniae was found to be a function of the pH; lysis was suppressed if the pH of the pneumococcal culture was below 6.0 during penicillin treatment. In the case of B. subtilis, growth at pH 6.6 prevented penicillin-induced lysis. In pneumococci, the addition of trypsin to the growth medium also protected against lysis. The pH-dependent protection phenomenon resembled in several respects the antibiotic "tolerance" of pneumococci with a defective autolytic system. (i) At the pH nonpermissive for lysis, the bacteria retained their normal sensitivity to beta-lactam and to other cell wall inhibitors; however, instead of lysis, the drug-treated bacteria simply stopped growing. Loss of viability of the cells was also greatly reduced. (ii) Protection against lysis was independent of the dose and chemical nature of the cell wall inhibitors. (iii) The protection effect was reversible; lysis and loss of viability could be triggered by a postincubation of the drug-treated bacteria at the pH permissive for lysis.  (+info)

The autolysis loop of activated protein C interacts with factor Va and differentiates between the Arg506 and Arg306 cleavage sites. (4/238)

The anticoagulant human plasma serine protease, activated protein C (APC), inactivates blood coagulation factors Va (FVa) and VIIIa. The so-called autolysis loop of APC (residues 301-316, equivalent to chymotrypsin [CHT] residues 142-153) has been hypothesized to bind FVa. In this study, site-directed mutagenesis was used to probe the role of the charged residues in this loop in interactions between APC and FVa. Residues Arg306 (147 CHT), Glu307, Lys308, Glu309, Lys311, Arg312, and Arg314 were each individually, or in selected combinations, mutated to Ala. The purified recombinant protein C mutants were characterized using activated partial thromboplastin time (APTT) clotting assays and FVa inactivation assays. Mutants 306A, 308A, 311A, 312A, and 314A had mildly reduced anticoagulant activity. Based on FVa inactivation assays and APTT assays using purified Gln506-FVa and plasma containing Gln506-FV, it appeared that these mutants were primarily impaired for cleavage of FVa at Arg506. Studies of the quadruple APC mutant (306A, 311A, 312A, and 314A) suggested that the autolysis loop provides for up to 15-fold discrimination of the Arg506 cleavage site relative to the Arg306 cleavage site. This study shows that the loop on APC of residues 306 to 314 defines an FVa binding site and accounts for much of the difference in cleavage rates at the 2 major cleavage sites in FVa. (Blood. 2000;96:585-593)  (+info)

The D-alanine residues of Staphylococcus aureus teichoic acids alter the susceptibility to vancomycin and the activity of autolytic enzymes. (5/238)

Recently, Staphylococcus aureus strains with intermediate resistance to vancomycin, the antibiotic of last resort, have been described. Multiple changes in peptidoglycan turnover and structure contribute to the resistance phenotype. Here, we describe that structural changes of teichoic acids in the cell envelope have a considerable influence on the susceptibility to vancomycin and other glycopeptides. S. aureus cells lacking D-alanine esters in teichoic acids exhibited an at least threefold-increased sensitivity to glycopeptide antibiotics. Furthermore, the autolytic activity of the D-alanine mutant was reduced compared to the wild-type, and the mutant was more susceptible to the staphylolytic enzyme lysostaphin. Vancomycin inhibited autolysis at very high concentrations but neither in the wild-type nor in the mutant was the autolytic activity influenced in the range of the MIC. Mutant cells had a considerably higher capacity to bind vancomycin.  (+info)

Description of staphylococcus serine protease (ssp) operon in Staphylococcus aureus and nonpolar inactivation of sspA-encoded serine protease. (6/238)

Signature tagged mutagenesis has recently revealed that the Ssp serine protease (V8 protease) contributes to in vivo growth and survival of Staphylococcus aureus in different infection models, and our previous work indicated that Ssp could play a role in controlling microbial adhesion. In this study, we describe an operon structure within the ssp locus of S. aureus RN6390. The ssp gene encoding V8 protease is designated as sspA, and is followed by sspB, which encodes a 40.6-kDa cysteine protease, and sspC, which encodes a 12.9-kDa protein of unknown function. S. aureus SP6391 is an isogenic derivative of RN6390, in which specific loss of SspA function was achieved through a nonpolar allelic replacement mutation. In addition to losing SspA, the culture supernatant of SP6391 showed a loss of 22- to 23-kDa proteins and the appearance of a 40-kDa protein corresponding to SspB. Although the 40-kDa SspB protein could degrade denatured collagen, our data establish that this is a precursor form which is normally processed by SspA to form a mature cysteine protease. Culture supernatant of SP6391 also showed a new 42-kDa glucosaminidase and enhanced glucosaminidase activity in the 29 to 32 kDa range. Although nonpolar inactivation of sspA exerted a pleiotropic effect, S. aureus SP6391 exhibited enhanced virulence in a tissue abscess infection model relative to RN6390. Therefore, we conclude that SspA is required for maturation of SspB and plays a role in controlling autolytic activity but does not by itself exert a significant contribution to the development of tissue abscess infections.  (+info)

Increasing the thermal stability of euphauserase. A cold-active and multifunctional serine protease from Antarctic krill. (7/238)

A molecular model of Antarctic krill euphauserase based on the known crystal structure of its fiddler crab analog, collagenase I, indicates that the core structure of these enzymes is almost identical. Euphauserase is a cold-active and thermally sensitive enzyme with a high affinity for Lys, Arg and large hydrophobic amino acids. Residue Phe137 in euphauserase, localized in loop D (autolysis loop), is highly exposed on the surface of the molecule. Therefore, it appeared to be an easy target for autolysis. The broadly specific euphauserase has a low affinity for negatively charged residues. In order to increase the stability of the enzyme, two mutants were created in which residue Phe137 was replaced by a Glu and an Asp residue. Both mutations resulted in increased stability of the recombinant euphauserase towards thermal inactivation.  (+info)

The AbcA transporter of Staphylococcus aureus affects cell autolysis. (8/238)

Increased production of penicillin-binding protein PBP 4 is known to increase peptidoglycan cross-linking and contributes to methicillin resistance in Staphylococcus aureus. The pbp4 gene shares a 400-nucleotide intercistronic region with the divergently transcribed abcA gene, encoding an ATP-binding cassette transporter of unknown function. Our study revealed that methicillin stimulated abcA transcription but had no effects on pbp4 transcription. Analysis of abcA expression in mutants defective for global regulators showed that abcA is under the control of agr. Insertional inactivation of abcA by an erythromycin resistance determinant did not influence pbp4 transcription, nor did it alter resistance to methicillin and other cell wall-directed antibiotics. However, abcA mutants showed spontaneous partial lysis on plates containing subinhibitory concentrations of methicillin due to increased spontaneous autolysis. Since the autolytic zymograms of cell extracts were identical in mutants and parental strains, we postulate an indirect role of AbcA in control of autolytic activities and in protection of the cells against methicillin.  (+info)

Global Yeasts, Yeast Extracts and Autolysates Market is valued at $6.65 billion in 2014 and is poised to reach $12.54 billion by 2022 growing at a CAGR of...
The trypsin used was side-chain protected porcine trypsin from Promega. The enzyme was derivatized by reductive methylation (Lys side-chains) to prevent autolysis. The data suggest that it underwent some autolysis prior to and/or during the derivatization. The peptides in bold were identified by LC/MS/MS experiments. The Cys residues participate in disulfide-linkages. The list contains only N-terminally methylated or doubly methylated or Lys side-chain Me-derivatives. ...
Dive into the research topics of ADAM19 autolysis is activated by LPS and promotes non-classical secretion of cysteine-rich protein 2. Together they form a unique fingerprint. ...
Cysteine protease involved in xylem tracheary element (TE) autolysis during xylogenesis in roots. Participates in micro autolysis within the intact central vacuole before mega autolysis is initiated by tonoplast implosion.
Elucidating the origin of the surface functionalization - dependent bacterial toxicity of graphene nanomaterials: Oxidative damage, physical disruption, and cell autolysis ...
Activation of skeletal muscle calpain-3 by eccentric exercise in humans does not result in its translocation to the nucleus or cytosol. J Appl Physiol (1985). 2011 Nov; 111(5):1448-58 ...
The surface charge of yeast cells was found to be determined by the design and parameters of the fermentation process. Cells may be emptied by autolysis/plasmolysis, extraction methods which have proven effective in industry as well, to obtain hollow yeast cell envelopes comprised of cell walls only. The extraction processes render the yeast cells inactive and transform them into yeast cell envelopes, washing out intracellular low-molecular substances whilst maintaining the shape of the cells. Even though the cell�s zeta potential � main criterion for assessing their suitability for coating in subsequent precipitation processes � goes down during autolysis/plasmolysis, it is still sufficient for the requirements of this application ...
Words starting with A (page 114): autokinesis, autokinetic, autolatries, autolatry, autoload, autoloader, autoloading, autologies, autologous, autology, autolysate, autolyse, autolysed, autolyses, autolysin, autolysing, autolysins, autolysis, autolytic, autolyzate...
Defects in the formation of the septum and gradually autolysis of cells occur when the dapdependent mutant ofEscherichia coli is grown in a medium with 4-hydroxy-diaminopimelic... ...
The potential of the application of PEF to induce accelerate autolysis of a commercial strain of Sacharomyces cerevisiae for winemaking use was evaluated. The influence of PEF treatments of different intensity (5-25 kV/cm for 30-240 µs) on cell viability, cytoplasmic membrane permeabilization, release of mannoproteins and compounds absorbing at 260 and 280 nm has been investigated.After 8 days of incubation at 25 ºC the Abs600 of the suspension containing the control cells was kept constant while the Abs600 of the suspension containing the cells treated by PEF decreased. The measurement of the absorbance at 260 and 280 nm revealed no release of UV absorbing material from untreated cells after 8 days of incubation but the amount of UV absorbing material released drastically increased in the samples that contained cells treated by PEF after the same storage period. After 18 days of storage the amount of mannoproteins released from the untreated cell was negligible. Conversely, mannoprotein concentration
China The reason of beer yeast autolysis Suppliers,products - The fermentation process is actually the yeast metabolism process, to obtain high-quality beer, must first have a strong vitality, its own strong, good performance, flavor beneficial yeast strains. And yeast performance by the fermentation process conditions and the appearance of environmental facto...
05 Proline The dominating amino acids in both cropping types were cysteic acid, citrulline, proline, phenylalanine, and taurine. 7). An accumulation of proline under continuous cropping system was reported by Życzyńska-Bałoniak et al. (1986). The accumulation of proline under continuous cropping of rye conditions may be due to its heterocyclic amino acid structure, which could be more resistant to microbial degradation than other amino acids. The accumulation of proline in soils is considered as negative effect. The polymers of HA are synthesized enzymatically within microbial cell by chemical oxidative condensation following cellular autolysis. Therefore, HA are rarely homogenous; however, they represent accumulation of more resistant end products from many reaction taking place under natural conditions, either directly or indirectly through biological processes. ) in which it is formed (Piccolo et al. 2003). HA provides energy to many biochemical processes in soil (Dziadowiec 1979; ...
1. The administration of dihydrotestosterone to rats orchidectomized 7 days previously stimulated the synthesis of nuclear receptor in prostatic cells several hours in advance of DNA synthesis and mitosis. 2. The synthesis of nuclear receptor is tightly coupled to cell proliferation; consequently, in resting cells, there is no further net synthesis of nuclear receptor above the maximum of approx. 8000 molecules/cell. 3. After orchidectomy a rapid decline in the concentration of free androgen in the nuceus and a slower decline in the concentration of nuclear receptor are observed. 4. Owing to the apparent scarcity of receptor-inactivating factors in the nucleus, and the inverse relationship between amounts of nuclear and cytoplasmic receptors, it is concluded that the nuclear receptor is discharged into the cytoplasm after orchidectomy. 5. The formation of the cytoplasmic receptor is an early event preceding the onset of cellular autolysis. 6. Regressing prostate develops the capacity to ...
BCC Research (Wellesley; MA), a market research group, predicts the global market for yeast, yeast extract and autolysates will increase at a compound annual growth rate (CAGR) for 7.6% to $4.7...
Its all about the synonyms. Why say sweet when you can say residual malt sugars? Who needs alcoholic when you can discuss ABV? Brewers are shy and timid creatures really, and one word may be enough to gain their attention and respect if suitably technical. Swirl the glass around in your hand, sniff and grunt: Hmm. Hint of autolysis. Or: interesting - redolent of phenols. Or: spot of diacetyl. They will be in awe of your abilities. After a few drinks, the word you use wont even matter: frown, adopt a serious town, and say: Ah, theres a bit of dialysis in here. Everyone else will nod. You will end the evening either sounding like a knowledgeable critic or an intelligent enthusiast. Either way, its all good ...
vasokonstriksi  dilatasi  hemorraghia subepielial  membtk hematoma di lapisan superfisial endomet  rupturnya lapisan atas endomet  jaringan endomet rusak dilepas bersama darah sbg darah haid. Fragmen jaringan endomet dapat dikenali pd darah haid meskipun tjd autolysis  perdarahan berhenti bila arteri spiralis mengalami vasokonstriksi  permukaan endomet tertutup o/ epitel yg tumbuh dr sisa epitel kelenjar ...
vasokonstriksi  dilatasi  hemorraghia subepielial  membtk hematoma di lapisan superfisial endomet  rupturnya lapisan atas endomet  jaringan endomet rusak dilepas bersama darah sbg darah haid. Fragmen jaringan endomet dapat dikenali pd darah haid meskipun tjd autolysis  perdarahan berhenti bila arteri spiralis mengalami vasokonstriksi  permukaan endomet tertutup o/ epitel yg tumbuh dr sisa epitel kelenjar ...
The Human Factory has written and spoken worldwide about the underappreciated issue of health blindness, that around 6% of medical personnel are severely handicapped at seeing veins and clinical signs that others take for granted. Human Factory innovati
Yamagata H.; Ueno S.; Iwasaki T., 1989: Isolation and characterization of a possible native cucumisin from developing melon fruits and its limited autolysis to cucumisin
A cadaver, also called corpse (singular) in medical, literary, and legal usage, or when intended for dissection, is a deceased body. Observation of the various stages of decomposition can help determine how long a body has been dead. The first stage is autolysis, more commonly known as self-digestion, during which the bodys cells are destroyed through the action of their own digestive enzymes. However, these enzymes are released into the cells because of active processes ceasing in the cells, not as an active process. In other words, though autolysis resembles the active process of digestion of nutrients by live cells, the dead cells are not actively digesting themselves as is often claimed in popular literature and as the synonym of autolysis self-digestion seems to imply. As a result of autolysis, liquid is created that gets between the layers of skin and makes the skin peel off. During this stage, flies (when present) start to lay eggs in the openings of the body: eyes, nostrils, mouth, ...
We originally identified AtlA as a predicted surface protein that was required for maturation of biofilms (10). Disruption of atlA also resulted in resistance to autolysis and excessive chaining of cells (10, 69), and Shibata et al. (66) determined that the protein had peptidoglycan hydrolase activity in zymograms. Our present study reveals that AtlA is also required for biogenesis of a normal cell surface and full expression of genetic competence by S. mutans. Thus, AtlA appears to play a central role connecting cell wall remodeling, biofilm formation, genetic competence, and autolysis. These networks have often been shown to overlap with the stress regulon, but the AtlA protein does not appear to be required for acid tolerance, since no obvious differences were observed in the growth rate of the atlA mutant in acidified BHI broth (pH 6.4 or 5.4) (data not shown). However, it is possible that AtlA may be involved in other stress responses, because autolysin-mediated cell wall turnover is known ...
No, the science behind juice fasting is similiar to water fasting. In both ventures your body slips into a state of autolysis, a natural state of repair where digestion stops, which is why people don t die from fasting but will quickly die from starvation. Look into books like, The Juice Fasting Bible or How to keep Slim, Healthy and Young through Juice Fasting . Both were written by MDs.
Synonyms for autolytic in Free Thesaurus. Antonyms for autolytic. 1 synonym for autolysis: self-digestion. What are synonyms for autolytic?
Two courses of reinduction chemotherapy followed by bone marrow biopsy and aspirate prior to peripheral blood stem cell (PBSC) harvest. If marrow involvement is still present at harvest, then 2 additional courses of induction chemotherapy are given. The PBSC transplantation preparative regimen should begin within 2 weeks of completing reinduction therapy course, consisting of the following: Carmustine IV over 3 hours on days -8, -7, and -6, Etoposide continuous IV over days -8, -7, and -6, Cyclophosphamide IV over 1 hour daily on days -5, -4, -3, and -2, Mesna as a 15 min infusion before each dose of cyclophosphamide then at 3, 6, 9, and 12 hours after initiation of each cyclophosphamide dose Methylprednisolone IV is given to protect lungs from the toxic effects of carmustine ...
Minimise temperature shocks. If a refrigerant temperature of close to zero degrees Celsius is capable of controlling the fermentation process, it would certainly be of benefit to the growing yeast cells, which would not be subjected to temperature shock if the refrigerant were at a lower temperature. Similarly the cone temperature, which is often controlled at -4 degrees Celsius, could be contributing significantly to yeast autolysis at the interface. Consideration should also be given to a two-phase water chiller for reducing the temperature of recovered yeast, rather than the sudden reduction of temperature using propylene glycol or compressed ammonia at very low temperatures. The rate of chillback of the fermentation should also be prolonged to ensure that the possibility of temperature shock is minimised ...
Disclosed is a controlled autolysis method for making biological tissue substantially acellular by exposing the biological material, prior to any fixation thereof, to at least one buffered solution having a pH in the range from about 5.0 to 8.0 and a temperature in the range from about 12 C. to 30 C. for a sufficient period of time to render at least one region of the biological material substantially acellular while substantially preserving the structural integrity and non-cellular structural components of the biological material. Also disclosed is a method of making a bioprosthetic heart valve using biological material that has been treated by controlled autolysis and a method of treating a mammal having a defective heart valve using a bioprosthetic heart valve made, in part, by controlled autolysis.
Synonyms for Cell lysis in Free Thesaurus. Antonyms for Cell lysis. 19 words related to lysis: convalescence, recuperation, recovery, biochemistry, autolysis, self-digestion, bacteriolysis, cytolysis, dissolution, disintegration. What are synonyms for Cell lysis?
niger during submerged cultivation. The impact of secondary growth by carbon recycling was indicated by hyphal population dynamics illustrating a gradual transition from old to young hyphae. The induc tion of autophagic and reproductive processes was clearly evident by major genome wide transcriptional trends. Hydrolases with strong transcriptional induc tion during carbon starvation include ChiB, NagA, AgnB, PepA and PepB. Importantly, fragmentation of empty hyphal ghosts was not observed, thus constitut ing direct evidence that autolysis in aging submerged cultures of A. niger is rather initiated Inhibitors,Modulators,Libraries by cell death than by hydrolytic weakening and fragmentation of cell walls. Methods Strain, inoculum and media compositions Conidial suspensions for inoculation of bioreactor cul tures Inhibitors,Modulators,Libraries were prepared by growing the A.. niger laboratory strain N402 on solid i?ed complete medium for three days at 30 C in the dark. Spores were harvested with ...
niger during submerged cultivation. The impact of secondary growth by carbon recycling was indicated by hyphal population dynamics illustrating a gradual transition from old to young hyphae. The induc tion of autophagic and reproductive processes was clearly evident by major genome wide transcriptional trends. Hydrolases with strong transcriptional induc tion during carbon starvation include ChiB, NagA, AgnB, PepA and PepB. Importantly, fragmentation of empty hyphal ghosts was not observed, thus constitut ing direct evidence that autolysis in aging submerged cultures of A. niger is rather initiated Inhibitors,Modulators,Libraries by cell death than by hydrolytic weakening and fragmentation of cell walls. Methods Strain, inoculum and media compositions Conidial suspensions for inoculation of bioreactor cul tures Inhibitors,Modulators,Libraries were prepared by growing the A.. niger laboratory strain N402 on solid i?ed complete medium for three days at 30 C in the dark. Spores were harvested with ...
Cant believe it. In Russia we say u durakov mysli odinakovye (fools share their mind on air). I have the same article been opened maybe since Feb.2017 (and its opened just now). On one hand, the most of yeast is inactive after first 48 hours (being dead or sick, hehe). Due to its sickness (lo-sugars + hi-alco + progressing autolysis), the colony starts to make more and more by-products. But the U.S. tradition tends to have hi-nitro wort in the very beginning (sour mash or dunder or rum bomb + the non-filtered mash fermentation). And... I didnt taste much of American malt or corn based beverages but all I know of have put a long-long tail into my nose. In contrast to it, Islay is rich in powerful esters, maybe some feinty notes but not so definite tails. Longer aging only? Secret Gaelic strains? AFAIK, they filter the mash prior to pitching the yeast. No nitro-vitamin-bombs or porridge or backset inside. And SG 1.05..1.06. They make sorta ale without hops, in fact. But still there ...
An impeccable sample fixation is a prerequisite for a correct histological diagnosis. Tissue samples must be immersed in an optimally chosen fixative immediately after sampling, because a timely fixation will prevent autolysis, putrefaction and other unwanted cellular changes. Although there are hundreds of histological fixatives and at least tens of formaldehyde-based fixatives, neutral buffered formaldehyde solutions with a concentration range from 4% to 10% are the most commonly used fixatives, primarily because of their simple and universal application. Tissue fixation using a buffered formaldehyde solution results in forming cross-links, i. e. it forms methylene bridges between proteins, that is, it results in keeping tissue components in their in vivo relation. If fixated properly, the tissue sample can withstand additional histological tissue processing and staining.. The NB 4% formaldehyde solution is 10% buffered formalin, which is the most commonly used fixative. It is suitable for ...
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TÜ Molekulaar- ja rakubioloogia instituudis kaitstud 3+2 õppekava magistritööd aastal 2010: Külliki Püvi Pseudomonas putida glükoosist sõltuvat autolüüsi mõjutavad geenid Genes affecting glucose-dependent autolysis of Pseudomonas putida Juhendaja: vanemteadur Rita Hõrak, PhD, TÜ Molekulaar- ja Rakubioloogia Instituut Retsensent: vanemteadur Niilo Kaldalu, PhD, TÜ
A coordinated action of murein synthetases and murein hydrolases is assumed to be essential for proper growth of the bacterial cell. A disturbance of this balance, for example by blocking parts of...
The acid-acting proteinase, cathepsin D (EC 3.4.4.23), was purified from extracts of homogenized rabbit lung and beef lung by autolysis at acid pH, acetone and ammonium sulfate fractionation, column chromatography, and isoelectric focusing. Four isoenzymes were obtained from each source. With acid hemoglobin as the substrate, the proteinase from rabbit lung had a pH optimum of 3.0 and that from beef lung had a pH optimum of 3.6. Their activity was not affected by thiol reagents or by Fe2+, Mn2+, or Mg2+. One isoenzyme from rabbit lung was used to immunize a goat, and one from beef lung was used to immunize a rabbit. In immunoelectrophoresis, each resulting antiserum formed a single precipitin line with its homologous enzyme. They cross-reacted with the other three isoenzymes from the same species, but not with any isoenzyme from the other species. At high concentrations, each antiserum completely inhibited the proteolytic activity of its homologous enzyme. The antiserum against rabbit lung ...
All (3-Iactarn drugs like penicillins, cephalosporins are selective inhibitors of bacterial cell wall synthesis and therefore active against growing bacteria. Mechanism of action: Initial step is the binding of the drug to specific drug receptor PBPs (Penicillin- binding proteins) on bacteria. There are 3 to 6 PBPs having different effects. At least some of which are enzymes involved in transpeptidation (cross-linking) reactions. After attachment, peptidoglycan synthesis is inhibited as final transpeptidation is blocked. Then there occurs inactivation of an inhibitor of autolytic enzyme in the cell wall. This activates the autolytic enzymes in the cell wall that results in lysis resulting in bacterial death. Organisms with defective autolysin function are inhibited but not killed by l3dactam drugs, and they are said to be tolerant ...
Dr. Akeisha Belgrave conducted research in Cell Analysis and Modeling and Biophysics at the University of Connecticut Health Center and Princeton University as a doctoral student and postdoctoral student respectively. She conducted extensive studies on the shape maintenance and motility of rod-shaped bacteria.. Dr. Belgrave is currently working with undergraduate students on their experiential projects and independent research at the university to probe aquatic biofilms towards a healthy ecosystem and also to determine the effects of bacterial cell wall turnover towards a change in antibiotic efficacies, motility, and cell shape.. ...
The lysosomes are used for the digestion of macromolecules from phagocytosis (ingestion of other dying cells or larger extracellular material), endocytosis (where receptor proteins are recycled from the cell surface), and autophagy (where old or unneeded organelles or proteins, or microbes which have invaded the cytoplasm are delivered to the lysosome). Autophagy may also lead to autophagic cell death, a form of programmed self-destruction, or autolysis, of the cell, which means that the cell is digesting itself. Other functions include digesting foreign bacteria (or other forms of waste) that invade a cell and helping repair damage to the plasma membrane by serving as a membrane patch, sealing the wound. Lysosomes also do much of the cellular digestion required to digest tails of tadpoles and to remove the web from the fingers of a 3-6 month old fetus. This process of programmed cell death is called apoptosis.[1] ...
To maintain the tissue in as lifelike a state as possible, tissue for analysis is usually placed directly into a fixative solution upon removal from the body. Fixation is normally carried out as soon as possible to prevent autolysis and to reduce possible infectivity. Several factors determine the choice of fixative for a given application. If morphological changes are known to take place rapidly after tissue collection-as with neural tissue-the speed of fixing action is extremely important.. For large tissue samples, the rate of penetration of the fixative into the sample must also be considered. If immunological detection methods are to be used, a fixative which preserves protein structure is required. For electron microscopy, fixatives which do not precipitate proteins avoid artifacts invisible to light microscopy. The price of a fixative may also be a factor. The low cost of formaldehyde fixatives is one reason for their popularity. A partial list of fixatives would include (grouped by ...
Im told that they produce house brands for some Bay Area restaurants, but I dont have any specifics. They recently moved from Lagunitas to their new digs in Petaluma CA. Their other beers include a Rye Porter (seasonal), Lagunitas Gold and a Peach Wheat (seasonal). A big mahalo to Lagunitas own and for sending most of the bottled beers Ive reviewed here. The labels alone are worth the price of the bottle...the excellent beers are a bonus!. We at the Lagunitas Brewing Company believe in the three Great Principles of Craft Brewing. 1) Always be true to your school. 2) Never use the word Autolysis in public. 3) Be obsequious, and live in a swamp.. ...
Im looking for tumors in the small and large intestines of mice. Ive been inflating the intestines with formalin immediately after removal to prevent autolysis. It also stretches the intestines making them easier to cut open and, if things work well, flushes the lumen. However, there are often tight constricted areas that do not inflate or flush. I was thinking that things would be simple if the intestines were flaccid in the first place. Does anyone have a technique to relax the intestines prior to flushing with formalin? Claude Nagamine MIT, Div. Comp. Medicine Cambridge, MA _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ...
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[169 Pages Report] Specialty Yeast Market report categorizes the global market by Species (Saccharomyces Cerevisiae, Pichia Pastoris, Kluyveromyces), Application, Type (Yeast Extracts, Yeast Autolysates, Yeast Beta-glucan, and Other Yeast Derivatives), and by Region
de Eugenio, L.I., García, P., Luengo, J.M., Sanz, J., San Román, J., García, J.L., and Prieto, M.A. (2007) Biochemical evidence that phaZ gene encodes a specific intracellular medium chain length polyhydroxyalkanoate depolymerase in Pseudomonas putida KT2442: characterization of a paradigmatic enzyme. J Biol Chem 282: 4951-4962 ...
Although the criteria used in the macroscopic evaluation of cholecystectomy specimens are similar in all the pathological departments, there may be interdepartmental differences in macroscopic sampling. Meticulous macroscopic evaluation and sampling constitute the first steps to correct histopathological diagnosis. Histopathologically, the mucosa of the gallbladder may not always be observed throughout the sample taken due to autolysis, mucosal denudation, or curling. Because the epithelium of gallbladder is quite susceptible to bile-related autolysis, prompt fixation of gallbladder samples is advisable.1 Thus, embedding of the samples must be careful and precise. In this study, the sample taken longitudinally was rolled with the swiss-roll method in almost all cases in the 2nd group. However, POM was found to be similar in both groups. This result indicates that rolling the sample with the swiss-roll method was not a solution to the problem that the mucosa could not be observed throughout ...
Inactivated yeast or semi-purified fractions of it have been used as additives for animal and fish feeds. These preparations have been proposed to encourage animal growth and health by various mechanisms, including immunomodulation, oxidative status, binding of toxins and pathogens, and interactions with gut ingredients.. Inactivated yeast or yeast autolysate contains the water-soluble and insoluble components of the yeast cell, the composition of which is primarily amino acids, peptides and carbohydrates. Yeast autolysate is manufactured by breaking down the cells using endogenous or exogenous enzymes. There have been many reports on the manufacturing processes.. At the end of yeast fermentation, yeast cells were separated by centrifugation from the spend medium and washed twice with process water and stored in large storage tanks at 4 oC. First step for the manufacturing of Inactivated Yeast is the cell inactivation, disruption and autolysis. For that purpose several methods may be ...
I believe that I may have found something truly original and worthwhile that might be done in the New World.1 Its worthwhile not just because it is novel-this is the idea of growing grapes from seeds-but because I think that it can create a real paradigm shift in how we experience wine.. Broadly speaking, the qualities that we experience in wine come from three major sources-you can almost conceive of them as radio signals of greater or lesser strength: 1) The inherent qualities of the site itself (its terroir); this is potentially the strongest signal, but it can also be quite obscured by grapegrowing and winemaking practices, drip irrigation most notably; 2) the characteristics imparted by the selection of the plant material-rootstock and scion, from the ripening properties of the vines themselves to the flavor profile of the grape varieties; 3) the overlay of winemaking technique-barrel character, diacetyl or malolactic character, lees autolysis, the qualities imparted by designer yeasts ...
Ian, Ive had much luck sith a 11.5 Kb low copy (pSC101) plasmid. I grow in TB terrific broth as described in the new Maniatis (24g YE, 12g Tryp, glycerol, PO4 buffer) with plenty of aeration (tube roller). A standard alkaline lysis followed by neutralizing with a half volume of 7.5 M ammonium acetate will do the job. When a 1.5 ml miniprep is resuspended in 50 ul, 5 ul will give a nice digest on an agorose gel. One trick you should try is to freeze your resuspended cell pellet after you have added lysozyme in the first step of your miniprep. This seems to have a marginal beneficail effect with DH5 as a host, but may be more important with other strains. I saw it in the inital report of pGB1, and it is a well know method of unleashing autolytic activity in one of my favorite organisms, Staph. aureus. J. Graham Biology and Chemistry Departments Indiana University -Bloomington Jim J. GrahaUniversity -Bloomington ...............It is necessary to be slightly underemployed if .............one is ...
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0007] One reason for the versatility of Ps. aeruginosa is that it produces a diverse battery of virulence determinants including elastase, LasA protease, alkaline protease, rhamnolipids, type IV pilus-mediated twitching motility, pyoverdin (Williams et al., 1996, Stintzi et al., 1998, Glessner et al., 1999), pyocyanin (Brint & Ohman, 1995, Reimmann et al., 1997) and the cytotoxic lectins PA-I and PA-II (Winzer et al., 2000). It is now known that many of these virulence determinants are regulated at the genetic level in a cell density-dependent manner through quorum sensing. Ps. aeruginosa possesses two well characterised quorum sensing systems, namely the las and rhl (vsm) systems which comprise of the LuxRI homologues LasRI (Gambello & Iglewski, 1991) and RhlRI (VsmRI) (Latifi et al., 1995) respectively. LasI directs the synthesis of 3-oxo-C12-HSL (Passador et al., 1993, Pearson et al., 1994) whereas RhlI directs the synthesis of C4-HSL (Winson et al., 1995). The las and the rhl systems are ...
Founded in 2001, ACMA Clinic provides professional and comprehensive health care of holistic and natural medicine.. ACMA patients come from 50 states in U.S. and countries in North America, South America, Asia, Europe, etc. Most ACMA patients first started treatments with their family doctors, specialists, local hospitals, and leading hospitals in U.S. or other countries. When those treatments could not help them, they started ACMA treatment. As a result, when they came to ACMA, they had been suffering from various severe diseases and illnesses for years, and many of them had been regarded as incurable.. However, after ACMA treatments, most patients conditions start improving for the first time in their disease history. Patients are happy to see they are recovering and returning back to normal lives.. ...
Have you ever played draughts? I think its called checkers in America. Its a board game that requires you to move pieces known as men and kings to take an opponents pieces. The pieces are often made of wood or plastic and have indentations to make stacking easier.. Its these indentations that link the game of draughts to bacteriology.. Let me introduce the pneumococcus. Formally known as Streptococcus pneumoniæ. This is a Gram-positive diplococcus that forms α-hæmolysis on blood agar. This means the colonies look green when you hold the plate up to the light.. As the bacterial cells divide and the colony grows in size, autolysis occurs in the centre of the colony forming a dimple or umbilication. Some clever bacteriologist a long time ago thought the colonies looked like draughts pieces and so in many bacteriology textbooks, youll read about how the colonies look like draughts (or checkers) pieces.. The pneumococcus can cause many infections including pneumonia, meningitis, bacteræmia, ...
A anti-bacterial is really a substance representative which is used to eliminate the quantity of worthwhile organisms on pharmaceutic surfaces to a appropriate levels. Disinfectants have many different components offering scope of activity, mode of activity, and effectiveness. Some are bacteriostatic, in which the ability with the microbe populace to reproduce is halted. In such cases, the disinfectant could cause discerning and reversible adjustments to microbial cells by getting together with nucleic acids and suppressing nutrients, or permeating to the mobile phone walls. The moment the anti-bacterial is removed from contact with microbial tissues, the enduring microbial populace could very well increase. Other disinfectants are bactericidal in that they destroy microbe cells and bring about irreparable problems through diverse elements that come with structural harm to the mobile phone, cell lysis, and autolysis, resulting in loss or coagulation of cytoplasm. The exploitation of microbe and ...
Yeast protein hydrolysate may be considered as a good source of bioactive peptides. Yeast hydrolysate was prepared by two different physical-enzymatic and autolysis treatments to identify the most active angiotensin I-converting enzyme (ACE) inhibitory and antioxidant peptides. The most active hydrolysate was obtained after sonication-trypsin hydrolysis. The hydrolysate was subjected to fractionat ...
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The issue is that some owners discontinue heartworm meds and their animals build up large loads of heartworms. A heavy dose of heartworm meds may kill those worms. Those dogs then have a significant number of dead worms in their system. Some may let go and float down stream just like a blood clot. Others will hang on and undergo autolysis, that is they disintegrate and release non-dog proteins into the bloodstream. The dogs immune system responds to those foreign proteins just like a massive infection ...
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Effect of liver autolysis in vivo. Proc. Soc. Exp. Biol. Med., 26:304-5. With J. C. Ellis. Fatal effect of total loss of ... Liver autolysis in vivo. Arch. Surg., 20:8-16. With M. L. Montgomery, W. B. Matthews, and J. C. Ellis. Fatal effect of the ...
"Autolysis - and its effects on Champagne". Champagne Gallery. Archived from the original on 17 May 2016. Retrieved 17 May 2016 ...
... begins at the moment of death, caused by two factors: 1.) autolysis, the breaking down of tissues by the body's ... Visible changes caused by decomposition are limited during the fresh stage, although autolysis may cause blisters to appear at ... The general stages of decomposition are coupled with two stages of chemical decomposition: autolysis and putrefaction. These ...
Levin RE, Van Sickle C (1976). "Autolysis of high-GC isolates of Pseudomonas putrefaciens". Antonie van Leeuwenhoek. 42 (1-2): ... it has been observed that at least some species of bacteria with DNA of high GC-content undergo autolysis more readily, thereby ...
Heat denatures the proteolytic enzyme and prevents autolysis. Heat fixation cannot be used in the capsular stain method as heat ... One reason is to kill the tissue so that postmortem decay (autolysis and putrefaction) is prevented. Fixation preserves ... fixation is the preservation of biological tissues from decay due to autolysis or putrefaction. It terminates any ongoing ...
Coronopus squamatus) based on its volatile glucosinolate autolysis products". Biochemical Systematics and Ecology. 36 (10): 807 ...
... this triggers autolysis, during which the yeast self-destructs. The dying yeast cells are then heated to complete their ...
Some proteases are less active after autolysis (e.g. TEV protease) whilst others are more active (e.g. trypsinogen). Proteases ...
Wireman JW, Dworkin M (February 1977). "Developmentally induced autolysis during fruiting body formation by Myxococcus xanthus ...
Martínez, Virginia; García, Pedro; García, José Luis; Prieto, María Auxiliadora (2011-07-01). "Controlled autolysis facilitates ...
Cell Wall Plant Polysaccharide-degrading Enzymes in Autolysis of Botrytis Cinerea. Science Direct, July 1980. Web. 14 Nov. 2016 ...
The autolysis of P. freudenreichii has been suggested to contribute further to the flavor of the Emmental cheese. The ... Ostlie H (1995). "Autolysis of Dairy Propionibacteria: Growth Studies, Peptidase Activities, and Proline Production". Journal ... conditions leading to the autolysis of this bacterium are not well known. Propionibacterium freudenreichii was first discovered ...
They called this autolysis after Christian de Duve and Alex B. Novikoff. However Porter and Ashford wrongly interpreted their ...
Cole, Rosemary A. (1976). "Isothiocyanates, nitriles and thiocyanates as products of autolysis of glucosinolates in Cruciferae ...
Cole, Rosemary A. (1976). "Isothiocyanates, nitriles and thiocyanates as products of autolysis of glucosinolates in Cruciferae ...
Zare H, Moosavi-Movahedi AA, Salami M, Mirzaei M, Saboury AA, Sheibani N (March 2013). "Purification and autolysis of the ficin ...
Chou JS, Impens F, Gevaert K, Davies PL (July 2011). "m-Calpain activation in vitro does not require autolysis or subunit ...
Cole Rosemary A (1976). "Isothiocyanates, nitriles and thiocyanates as products of autolysis of glucosinolates in Cruciferae". ...
Ecological Advantages of Autolysis during the Development and Dispersal of Pseudoalteromonas tunicata Biofilms. Applied and ...
Autolysis and putrefaction also play major roles in the disintegration of cells and tissues. The human body is composed of ...
The last function of a lysosome is to digest the cell itself through autolysis. The spitzenkörper is a component of the ...
... significant cell autolysis had occurred, and cell definition was very poor. His brain was almost completely gone, leaving only ...
The hol-lys gene system therefore is believed to constitute a chromosomally-encoded autolysis system. The reaction probably ...
The sodium bicarbonate inhibits the autolysis of acetyl groups as well as inhibiting glycosyl bonds. Depending on the ...
Two of these RNAs, csRNA4 and csRNA5, have been shown to affect stationary-phase autolysis. Bacterial small RNA Halfmann, A; ...
Phytoplankton can also produce DOC by autolysis during physiological stress situations e.g., nutrient limitation. Other studies ...
It inhibits the absorption and autolysis of bones and thus leads to lowering of blood calcium. It inhibits bone salts from ...
"Autolysis and aging of Penicillium chrysogenum cultures under carbon starvation: Chitinase production and antifungal effect of ...
... leading them to autolysis and death. Visualization of hemolysis (UK: haemolysis) of red blood cells in agar plates facilitates ...
Callister used autolysis to break down the yeast cells from waste obtained from the Carlton & United brewery. Concentrating the ...
Autolysis may refer to: Autolysis (biology), the destruction (or lysis) of a cell by its own enzymes Autocatalysis, in ... in the production of commercial extract This disambiguation page lists articles associated with the title Autolysis. If an ... or catalyzes its own transformation into another compound Autolysis (alcohol fermentation), the complex chemical reactions that ...
... and controlled autolysis of E. coli for protein expression and purification. ... XJb Autolysis Glycerol Stock, 1 ml 500x L-Arabinose 1 ml. $119.90 - + ... Autolysis. XJb lysis efficiency is 10-20 % lower than XJa. For optimal lysis, more care needs to be taken when selecting the ... The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). It can be ...
Innovative methods of extracting mannoproteins from lees and accelerating autolysis ... Back to the item: Innovative methods of extracting mannoproteins from lees and accelerating autolysis ...
Microscopic examination of the colon revealed only postmortem autolysis. Postmortem pathology using the alpha-toxin PCR assay ...
Triton X-100-induced autolysis was significantly decreased by SH in S. aureus ATCC 25923, and quantitative bacteriolytic assays ... which suggested that SH may impede biofilm formation by reducing the expression of cidA to inhibit autolysis and eDNA release ... In contrast, the autolysis-deficient phenotype of 14-4 was correlated with both increased expression of negative autolysis ... The inhibition of autolysis by MOL was evaluated using quantitative bacteriolytic assays and zymographic analysis, and ...
... of animals and tissues or organs lost to a study because of autolysis. Autolysis in excess of this standard may be cause to ... Necropsy should be performed soon after an animal is sacrificed or found dead, so that loss of tissues due to autolysis is ... K. Autolysis:. Adequate animal husbandry practices should result in considerably less than 10% ... the animal should be refrigerated at a temperature that is low enough to prevent autolysis but not so low as to cause cell ...
The specimen should still be prepared in formalin to avoid autolysis; formalin does not remove lipids, whereas alcohol baths ...
... strain that has been engineered for protein autoinduction resulting in high titers while also providing cell autolysis and ... Product Description Rokes Autoinduction/Autolysis E. coli strain is a proprietary bacterial ... E. coli "Auto-Induction/Auto-Lysis" + mCherry Positive control plasmid. RSA-001 Regular price $149.00 USD ... Rokes Autoinduction/Autolysis E. coli strain is a proprietary bacterial strain that has been engineered for protein ...
... ... Can the development and autolysis of lactic acid bacteria influence the cheese volatile fraction? The case of Grana Padano / ... Can the development and autolysis of lactic acid bacteria influence the cheese volatile fraction? The case of Grana Padano / ...
Autolysis. In the process of autolysis the cells will consume themselves by using enzymes from the same cells. The enzymes are ... Insolubles separation. After the autolysis the insoluble part such as yeast cells, is separated from the water-soluble ...
Beginning autolysis.. Focus/foci, several, black-brown. Discolouration, black-brown.. Spontaneous death.Day 3 after treatment. ... Beginning autolysis.. Focus/foci, several, black-brown. Discolouration, black-brown.. Spontaneous death.Day 3 after treatment. ...
Autolysis and Apoptosis. Virus particle Isolation and Purification. PCR test fraud and misuse. CDC Covid PCR diagnostic test ...
Promotes autolysis and a moist wound environment; Aids in pain control and decreased inflammation; Good for Stages II to IV ... Promotes autolysis; Suitable for superficial and partial thickness wounds with minimum to moderate exudate ... Promotes autolysis; Suitable for Stage I and II ulcers; Used for noninfected wounds ...
Wireman, J. W. & Dworkin, M. Developmentally induced autolysis during fruiting body formation by Myxococcus xanthus. J. ...
... autolysis; hyperplasia; cyanobacteria; fungi; ciliates; arthropods; gastrodermal sloughing; epidermal sloughing; endolithic ...
"Autolysis and aging of Penicillium chrysogenum cultures under carbon starvation: Chitinase production and antifungal effect of ...
This process is known as autolysis. The molecules released are slowly transformed as they interact with those in the wine. ... Maturation on lees therefore combines both these processes - yeast autolysis and slow oxidation - which, together, will ...
Lively mousse on a tender palate bubbles with toasty, nutty autolysis. As it warms up, the mousse softens and shows a sunny ...
autolysis or digestion of self or ones own cells. Caput medusae. tortuous, unsightly varicosities spreading from the umbilicus ...
Its not carbonated yet, so its a bit early to taste the result, but at least Im certain it did not suffer from autolysis off ...
Herlihey, F. and A.J. Clarke (2016) Controlling autolysis during flagella insertion in Gram-negative bacteria. Adv Exp Med Biol ...
Believe me if it had gone through autolysis you would smell it. ...
Holocrine secretion entails breakdown of the entire sebaceous cell; lysosomal enzymes are responsible for this autolysis. Cell ...
I was trying to explain the autolyse method to my sister, whos a pathologist, and her eyes lit up: "Autolysis!" In her world ... Great article about the details of autolysis. I bake rye sourdough using a recipe Ive twiddled with for maybe 20 years. Its a ...
Autolysis is one pneumococcal process that can initiate host defense. Autolysis involves the bacteria digesting itself by means ... Autolysis of the pneumococcus (as well as antibiotic treatment) leads to the release of subcapsular bacterial products that are ... pneumoniae upon autolysis. Additionally, products from pneumococcal cell walls can trigger an inflammatory response in a host ( ...
  • Research shows that human decomposition begins just a few minutes after death via a process of self-digestion known as autolysis, with temperature, humidity, rain and the sun, among other factors, playing an important role in the process. (co.zw)
  • The onset is governed by a process called autolysis - or self-digestion. (uaf.edu)
  • After a few minutes, autolysis , or self-digestion, sets the stage for decomposition. (myiota.life)
  • Decomposition begins several minutes after death with a process called autolysis, or self-digestion. (garrilas.com)
  • Roke's Autoinduction/Autolysis E. coli strain is a proprietary bacterial strain that has been engineered for protein autoinduction resulting in high titers while also providing cell autolysis and nucleotide autohydrolysis capabilities to facilitate routine downstream workflows. (rokebio.com)
  • Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis. (google.com.co)
  • Over time, meat decays because of two major factors: bacterial growth and autolysis. (oodmag.com)
  • At the same time as bacterial activity is taking place, chemical changes within the meat bring about the release of cellular enzymes that initiate the breakdown of cells and tissues, a process called autolysis that starts immediately after death. (oodmag.com)
  • In the bush in warmer conditions, we don't have the luxury of hanging meat under ideal conditions, and warm weather can quickly bring the onset of bacterial growth and autolysis, leading to fast decay and rot. (oodmag.com)
  • In addition, 2 addi- in brain tissue suggests that USUV tissue samples was not successful be- tional unique amino acid substitutions might have a higher tropism for the cause of autolysis. (cdc.gov)
  • These dressings maintain a moist heallng environment, promoting formation of Granylation Tissue and autolysis of necrotic tissue. (mountainside-medical.com)
  • The buffer prevents autolysis that occurs under acid conditions and also prevents the development of coloration of the tissue caused by formalin pigment. (nationaldiagnostics.com)
  • Autolysis uses the body's own enzymes and moisture to re-hydrate, soften and finally liquefy hard eschar, necrotic tissue and slough. (enluxtra.com)
  • So different on the nose, a definite yeasty autolysis, such a pleasing breadth and depth of aroma and flavour, very fine mousse and a delicious length and balance. (wine-pages.com)
  • Ask a wine maker from the Champagne region about the input to aroma and flavour of yeast autolysis and you will hear that it's completely misunderstood and grapes are what the flavour is all about. (chrisshanahan.com)
  • Fetal death and ensuing autolysis soften the tissues. (blogspot.com)
  • When tissues are removed from the body, the process of autolysis occurs rapidly. (ucsd.edu)
  • Flavors that can also be provided by yeast extracts, if the selection, fermentation and yeast autolysis are well controlled. (lesaffre.com)
  • During this aging process, the bottles are kept "en rima", or in a horizontal position, and are left to rest while they undergo a second fermentation. (cava.wine)
  • the fermentation in oak barrels is followed in the spring by the "en tirage" process, with the start of a slow second fermentation in bottles, according to the traditional Franciacorta method. (riccicurbastro.it)
  • I did the autolysis rest and the double fermentation thing. (kitchensaremonkeybusiness.com)
  • With 36 months of autolysis, it has sourdough, red berry, candy floss and sherbet flavours and well-integrated 7.4 grams of dosage. (kysela.com)
  • The best way to make dough with Sourdough is using the Autolysis technique. (biancolievito.com)
  • After the autolysis the insoluble part such as yeast cells, is separated from the water-soluble components by filtration. (vaisala.com)
  • and secondly there is a further overlay caused by contact of the wine with the expired yeast cells as they undergo an enzymatic breakdown (autolysis). (chrisshanahan.com)
  • Autolysis is the technical term for yeast cells breaking down in the wine when the Champagne is 'en lees' . (sipchampagnes.com)
  • Lively mousse on a tender palate bubbles with toasty, nutty autolysis. (wine.com)
  • It's a malty, comparatively dry style with lots of reserve wine richness, flavours of raspberry and milk chocolate, a hint of toasty autolysis and a creamy, well-balanced mid-palate. (timatkin.com)
  • This was done by comparing the optimum values of operating parameters such as the yeast concentration, processing temperature and the isolation time of each of the isolation methods used (Autolysis, Ultrasonic and Homogenizer). (scialert.net)
  • Unlike Ultrasonic and Homogenizer methods, the temperature for Autolysis method could be maintained within 1 h while for Homogenizer and Ultrasonic methods the temperature could only be maintained within 30 and 15 sec, respectively. (scialert.net)
  • Maturation on lees therefore combines both these processes - yeast autolysis and slow oxidation - which, together, will continue to hone the style of the wine, gracing it with so-called tertiary aromas of maturity and fullness. (champagne.fr)
  • Optimizing production processes (autolysis, microfiltration, drying, etc.) in general in conjunction with the group's industrial management. (lesaffre.com)
  • 2002 ) from filamentous fungi such as Aspergillus and Mucor species are suggested to have roles in processes such as hyphal autolysis, growth and branching. (springeropen.com)
  • Transcriptional and functional analysis shows sodium houttuyfonate-mediated inhibition of autolysis in Staphylococcus aureus. (omicsdi.org)
  • Transcriptional and functional analysis of the effects of magnolol: inhibition of autolysis and biofilms in Staphylococcus aureus. (omicsdi.org)
  • The inhibition of autolysis by MOL was evaluated using quantitative bacteriolytic assays and zymographic analysis, and antibiofilm activity assays and confocal laser scanning microscopy were used to elucidate the inhibition of biofilm formation caused by MOL in 20 clinical isolates or standard strains. (omicsdi.org)
  • RumenYeast is a pure yeast from Saccharomyces cerevisiae that goes through the autolysis process in which the end product consists of vitamins, peptides, free amino acids, and functional carbohydrates, such as MOS and β-glucans. (allaboutfeed.net)
  • The present work had for objective to optimize the autolysis of fresh brewery's yeast (Saccharomyces cerevisiae), aiming the maximum extraction of ribonucleic acid of biomass in the yeast extract production. (unesp.br)
  • Our yeast Extracts are manufactured by autolysis of a selected strain of Saccharomyces cerevisiae. (organotechnie.com)
  • Moreover, these IMERs enable less enzymatic autolysis, and the online in-flow digestion breaks the analysis time down to a few minutes or seconds compared to the time-consuming offline workflow that often requires overnight digestion (2-8,10-19). (chromatographyonline.com)
  • Wang, R., and Lynch, MD., Improved two‐stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease. (rokebio.com)
  • The palate brings substance to the slender but concentrated palate, and the lemony overtones are now enriched with buttery, lemony brioche while the fine mousse underlines the harmonious balance between ripe lemon freshness, slender poise, statuesque backbone and subtle autolysis. (wine.com)
  • Lovely autolysis and a palate that excites throughout. (cluboenologique.com)
  • The role of autolysis, they say is subtle and part of a larger comlex. (chrisshanahan.com)
  • The ageing of a sparkling wine during production 'en lees' to allow the prized flavour of autolysed yeast to develop in the wine. (sipchampagnes.com)
  • Aged on the lees for at least 36 months before release, this wine is expressive and complex, with notes of fruits and autolysis balanced by mineral notes and refreshing acidity. (thesavorygrape.com)
  • Also during this time, the wine's lees (yeast sediment) releases flavours in a process called autolysis. (thetaste.ie)
  • Triton X-100-induced autolysis was significantly decreased by SH in S. aureus ATCC 25923, and quantitative bacteriolytic assays and zymographic analysis demonstrated SH-mediated reduction of extracellular murein hydrolase activity in these cells. (omicsdi.org)
  • In the process of autolysis the cells will consume themselves by using enzymes from the same cells. (vaisala.com)
  • destruction of worn cells parts ('autolysis') and foreign particles? (studystack.com)
  • Autolysis is the breakdown of the body by endogenous substances. (medscape.com)
  • Meat-processing plants routinely take advantage of autolysis, a carefully controlled process called dry aging, to optimize meat tenderness. (oodmag.com)
  • Andrea Dal Cin, group technical director for Masi, is in charge of the production process here and draws on Masi's extensive experience to continuously improve the wines, one of the major programmes at the moment is experimenting with yeasts that will increase production of amino acids, to induce autolysis very quickly, thus protecting the wines from oxidation which in turn will minimise the use of sulphur. (wine-pages.com)
  • Confidently presented with complexity and poise, Altair wears a delicate sheen of savoury autolysis with almonds, baked pastry, poached pear, bright red berries aromas. (campaign-archive.com)
  • Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at -20°C. The presence of Ca 2+ will also diminish the self-autolysis of trypsin and maintain its stability in solution. (sigmaaldrich.com)
  • Worthington produces a chemically modified SequENZ® Trypsin with significantly reduced autolysis when used under extensive protein digests, along with specialty packaged trypsin, chymotrypsin, Endo-Arg-C and Endo-GLU-C for proteomics and protein sequencing applications. (worthington-biochem.com)
  • Still youthful by the autolysis-rich standards of some Colmant bubblies, this is a structured cuvée of Pinot Noir with 48% Chardonnay, showing raspberry, malt and citrus flavours, leesy underlying texture and a chalky flourish. (kysela.com)
  • The ripe fruit flavours of white stonefruit and shortbread enriched with yeast autolysis and brioche. (silverstreamwines.com)
  • Magnolol (MOL) shows inhibitory activity against S. aureus biofilms and Triton X-100-induced autolysis in vitro, although there are no data regarding the molecular mechanisms of MOL action in bacteria. (omicsdi.org)
  • The data suggested the involvement of two novel extracellular serine proteases, nodulin-like proteins and an Arabidopsis thaliana OPEN STOMATA 1 ( AtOST1 ) homolog in signaling fiber-cell death, as well as mechanisms responsible for hormonal control, nutrient remobilization, regulation of vacuolar integrity and autolysis of the dying fibers. (biomedcentral.com)
  • The complete digestion of a cell by its own mechanisms is called autolysis, and is a type of apoptosis (cell suicide). (biology-questions-and-answers.com)
  • Qamar, A. and Golemi-Kotra, D. Dual roles of FmtA in Staphylococcus aureus cell wall biosynthesis and autolysis. (enzyme-database.org)
  • Can the development and autolysis of lactic acid bacteria influence the cheese volatile fraction? (unipr.it)
  • 3 . In order to achieve the best healing outcome, the liquefied products of autolysis, as well as harmful bacteria, disintegrated biofilms, excessive salts, toxins, and other corrosive pro-inflammatory components of chronic wound exudate, must be continuously and non-traumatically evacuated from the wound . (enluxtra.com)
  • Highly absorbent inner layer effectively removes exudates from the wound and facilitates granulation, epithelization, and autolysis. (dentaldistrictsupply.com)
  • In most circumstances, autolysis and putrefaction occur in tandem. (medscape.com)
  • The growth of S. aureus strain ATCC 25923 in the presence of MOL dose-dependently led to decreases in Triton X-100-induced autolysis, extracellular murein hydrolase activity, and the amount of extracellular DNA (eDNA). (omicsdi.org)
  • The autolysis is carefully controlled to preserve the naturally occurring B-complex vitamins. (primelabmed.com)
  • The step down arrangement from best to worst for lab scale production was Autolysis>Homogenizer>Ultrasonic method. (scialert.net)
  • Autolysis was the best but the isolation time is longer compared to the other so that in large scale production, Homogenizer seems to be the best method due to the economical perspective. (scialert.net)
  • 1973. Autolysis and Chitinase Production in Cultures of Verticillium alboatrum. (ugm.ac.id)
  • Anti-biofilm assay showed that SH is poorly active against S. aureus grown in biofilm cultures, whereas SH diminished the amounts of extracellular DNA (eDNA) of S. aureus in a dose-dependent manner, which suggested that SH may impede biofilm formation by reducing the expression of cidA to inhibit autolysis and eDNA release in the early phase. (omicsdi.org)
  • The final phase in maturation of both vessel elements and fibers is cell death and autolysis of the cell contents. (biomedcentral.com)
  • In BHI + FCS, 106.66 shows the typical exponential phase followed by a peak OD 450nm followed by an autolysis phase. (biomedcentral.com)
  • In hCSF, 106.66 lacked the typical exponential phase, peak OD 450nm and the following autolysis phase. (biomedcentral.com)
  • But do I also want a longer autolysis phase? (chimpanzeeteaparty.com)
  • The process of autolysis in combination with 9.8% of NaCl, the RNA extraction yield in 24 hours was of 89.7%, with a yield of 51.3% in extract mass with 57.9% of protein and, 48.7% of dehydrated cell wall with 21.7% of protein. (unesp.br)
  • The longer this process of 'autolysis' persists the better, improving mouthfeel and longevity, and adding biscuity, bready nuances to the flavour. (tysonstelzer.com)
  • The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). (zymoresearch.com)
  • When glucose is added to the growth media, it inhibits the induction of the autolysis genes when it is present in the media. (zymoresearch.com)
  • A 1.9-Angstroms resolution crystal structure of free thrombin grown in the presence of high salt (400 mm KCl) shows two molecules in the asymmetric unit, one of which assumes an unprecedented conformation with the autolysis loop shifted 20 Angstroms away from its canonical position, the 220-loop entirely disordered, and the RGD sequence exposed to the solvent. (rcsb.org)
  • A study by Bonde et al indicated that the factor X autolysis loop takes part in all elements of factor X regulation. (medscape.com)
  • I was trying to explain the autolyse method to my sister, who's a pathologist, and her eyes lit up: "Autolysis! (kingarthurbaking.com)
  • Among the three methods, Autolysis method produced the highest GSH concentration of 49.263 μmol mL -1 (15.14 g L -1 ) followed by Homogenizer method (18.33 μmol mL -1 or 5.63 g L -1 ) which is slightly higher than that of the Ultrasonic method (18.13 μmol mL -1 or 5.57 g L -1 ). (scialert.net)
  • Will this be a problem as time goes by (autolysis? (homebrewersassociation.org)
  • When the time came to rack the vineyard designate to tank, I decided to satisfy a longtime curiosity by blending wine from these two new barrels into an older barrel for further surlie aging where yeast autolysis and further harmonization could occur. (scherrerwinery.com)
  • They are frequently nicknamed "suicide-bags" or "suicide-sacs" by cell biologists due to their role in autolysis. (phys.org)
  • Pathological-anatomical autopsy is the gold standard for determining of foetal abnormalities, but in some cases its role is limited (pathology of central nervous system, in particular, in case of ventricular dilatation or developed autolysis). (nel.edu)