Autolysis
Bacteriolysis
N-Acetylmuramoyl-L-alanine Amidase
Calpain
Cysteine proteinase found in many tissues. Hydrolyzes a variety of endogenous proteins including NEUROPEPTIDES; CYTOSKELETAL PROTEINS; proteins from SMOOTH MUSCLE; CARDIAC MUSCLE; liver; platelets; and erythrocytes. Two subclasses having high and low calcium sensitivity are known. Removes Z-discs and M-lines from myofibrils. Activates phosphorylase kinase and cyclic nucleotide-independent protein kinase. This enzyme was formerly listed as EC 3.4.22.4.
Cell Wall
Trypsinogen
Teichoic Acids
Cefotiam
Enteropeptidase
Staphylococcus aureus
Octoxynol
Endopeptidases
Muramidase
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
Enterococcus faecalis
Chymosin
Caseins
Phosphotungstic Acid
Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed)
Lysostaphin
Penicillins
A group of antibiotics that contain 6-aminopenicillanic acid with a side chain attached to the 6-amino group. The penicillin nucleus is the chief structural requirement for biological activity. The side-chain structure determines many of the antibacterial and pharmacological characteristics. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1065)
Streptolysins
Mutation
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Chloramphenicol
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
Clostridium perfringens
Foodborne Diseases
Acute illnesses, usually affecting the GASTROINTESTINAL TRACT, brought on by consuming contaminated food or beverages. Most of these diseases are infectious, caused by a variety of bacteria, viruses, or parasites that can be foodborne. Sometimes the diseases are caused by harmful toxins from the microbes or other chemicals present in the food. Especially in the latter case, the condition is often called food poisoning.
Fatal Outcome
Clostridium
Toxicity Tests
No-Observed-Adverse-Effect Level
Toxicity Tests, Chronic
Toxicity Tests, Acute
Functional Food
Autolysosomal membrane-associated betaine homocysteine methyltransferase. Limited degradation fragment of a sequestered cytosolic enzyme monitoring autophagy. (1/238)
We compared the membrane proteins of autolysosomes isolated from leupeptin-administered rat liver with those of lysosomes. In addition to many polypeptides common to the two membranes, the autolysosomal membranes were found to be more enriched in endoplasmic reticulum lumenal proteins (protein-disulfide isomerase, calreticulin, ER60, BiP) and endosome/Golgi markers (cation-independent mannose 6-phosphate receptor, transferrin receptor, Golgi 58-kDa protein) than lysosomal membranes. The autolysosomal membrane proteins include three polypeptides (44, 35, and 32 kDa) whose amino-terminal sequences have not yet been reported. Combining immunoblotting and reverse transcriptase-polymerase chain reaction analyses, we identified the 44-kDa peptide as the intact subunit of betaine homocysteine methyltransferase and the 35- and 32-kDa peptides as two proteolytic fragments. Pronase digestion of autolysosomes revealed that the 44-kDa and 32-kDa peptides are present in the lumen, whereas the 35-kDa peptide is not. In primary hepatocyte cultures, the starvation-induced accumulation of the 32-kDa peptide occurs in the presence of E64d, showing that the 32-kDa peptide is formed from the sequestered 44-kDa peptide during autophagy. The accumulation is induced by rapamycin but completely inhibited by wortmannin, 3-methyladenine, and bafilomycin. Thus, detection of the 32-kDa peptide by immunoblotting can be used as a streamlined assay for monitoring autophagy. (+info)Developmental aspects of secondary palate formation. (2/238)
Research on development of the secondary palate has, in the past, dealt primarily with morphological aspects of shelf elevation and fusion. The many factors thought to be involved in palatal elevation, such as fetal neuromuscular activity and growth of the cranial base and mandible, as well as production of extracellular matrix and contractile elements in the palate, are mostly based on gross, light microscopic, morphometric or histochemical observations. Recently, more biochemical procedures have been utilized to described palatal shelf elevation. Although these studies strongly suggest that palatal extracellular matrix plays a major role in shelf movement, interpretation of these data remains difficult owing to the complexity of tissue interactions involved in craniofacial development. Shelf elevation does not appear to involve a single motive factor, but rather a coordinated interaction of all of the abovementioned developmental events. Further analysis of mechanisms of shelf elevation requires development of new, and refinement of existing, in vitro procedures. A system that enables one to examine shelf elevation in vitro would allow more meaningful analysis of the relative importance of the various components in shelf movement. Much more is known about fusion of the palatal shelves, owing in large part to in vitro studies. Fusion of the apposing shelves, both in vivo and in vitro, is dependent upon adhesion and cell dealth of the midline epithelial cells. Adhesion betweeen apposing epithelial surfaces appears to involve epithelial cell surface macromolecules. Further analysis of palatal epithelial adhesion should be directed towards characterization of those cell surface components responsible for this adhesive interaction. Midline epithelial cells cease DNA synthesis 24-36 h before shelf elevation and contact, become active in the synthesis of cell surface glycoproteins, and subsequently manifest morphological signs of necrosis. Death of the midline epithelial cells is thought to involve a programmed, lysosomal-mediated autolysis... (+info)Suppression of the lytic and bactericidal effects of cell wallinhibitory antibiotics. (3/238)
The bacteriolytic effect of beta-lactam antibiotics on Bacillus subtilis and on Streptococcus pneumoniae was found to be a function of the pH; lysis was suppressed if the pH of the pneumococcal culture was below 6.0 during penicillin treatment. In the case of B. subtilis, growth at pH 6.6 prevented penicillin-induced lysis. In pneumococci, the addition of trypsin to the growth medium also protected against lysis. The pH-dependent protection phenomenon resembled in several respects the antibiotic "tolerance" of pneumococci with a defective autolytic system. (i) At the pH nonpermissive for lysis, the bacteria retained their normal sensitivity to beta-lactam and to other cell wall inhibitors; however, instead of lysis, the drug-treated bacteria simply stopped growing. Loss of viability of the cells was also greatly reduced. (ii) Protection against lysis was independent of the dose and chemical nature of the cell wall inhibitors. (iii) The protection effect was reversible; lysis and loss of viability could be triggered by a postincubation of the drug-treated bacteria at the pH permissive for lysis. (+info)The autolysis loop of activated protein C interacts with factor Va and differentiates between the Arg506 and Arg306 cleavage sites. (4/238)
The anticoagulant human plasma serine protease, activated protein C (APC), inactivates blood coagulation factors Va (FVa) and VIIIa. The so-called autolysis loop of APC (residues 301-316, equivalent to chymotrypsin [CHT] residues 142-153) has been hypothesized to bind FVa. In this study, site-directed mutagenesis was used to probe the role of the charged residues in this loop in interactions between APC and FVa. Residues Arg306 (147 CHT), Glu307, Lys308, Glu309, Lys311, Arg312, and Arg314 were each individually, or in selected combinations, mutated to Ala. The purified recombinant protein C mutants were characterized using activated partial thromboplastin time (APTT) clotting assays and FVa inactivation assays. Mutants 306A, 308A, 311A, 312A, and 314A had mildly reduced anticoagulant activity. Based on FVa inactivation assays and APTT assays using purified Gln506-FVa and plasma containing Gln506-FV, it appeared that these mutants were primarily impaired for cleavage of FVa at Arg506. Studies of the quadruple APC mutant (306A, 311A, 312A, and 314A) suggested that the autolysis loop provides for up to 15-fold discrimination of the Arg506 cleavage site relative to the Arg306 cleavage site. This study shows that the loop on APC of residues 306 to 314 defines an FVa binding site and accounts for much of the difference in cleavage rates at the 2 major cleavage sites in FVa. (Blood. 2000;96:585-593) (+info)The D-alanine residues of Staphylococcus aureus teichoic acids alter the susceptibility to vancomycin and the activity of autolytic enzymes. (5/238)
Recently, Staphylococcus aureus strains with intermediate resistance to vancomycin, the antibiotic of last resort, have been described. Multiple changes in peptidoglycan turnover and structure contribute to the resistance phenotype. Here, we describe that structural changes of teichoic acids in the cell envelope have a considerable influence on the susceptibility to vancomycin and other glycopeptides. S. aureus cells lacking D-alanine esters in teichoic acids exhibited an at least threefold-increased sensitivity to glycopeptide antibiotics. Furthermore, the autolytic activity of the D-alanine mutant was reduced compared to the wild-type, and the mutant was more susceptible to the staphylolytic enzyme lysostaphin. Vancomycin inhibited autolysis at very high concentrations but neither in the wild-type nor in the mutant was the autolytic activity influenced in the range of the MIC. Mutant cells had a considerably higher capacity to bind vancomycin. (+info)Description of staphylococcus serine protease (ssp) operon in Staphylococcus aureus and nonpolar inactivation of sspA-encoded serine protease. (6/238)
Signature tagged mutagenesis has recently revealed that the Ssp serine protease (V8 protease) contributes to in vivo growth and survival of Staphylococcus aureus in different infection models, and our previous work indicated that Ssp could play a role in controlling microbial adhesion. In this study, we describe an operon structure within the ssp locus of S. aureus RN6390. The ssp gene encoding V8 protease is designated as sspA, and is followed by sspB, which encodes a 40.6-kDa cysteine protease, and sspC, which encodes a 12.9-kDa protein of unknown function. S. aureus SP6391 is an isogenic derivative of RN6390, in which specific loss of SspA function was achieved through a nonpolar allelic replacement mutation. In addition to losing SspA, the culture supernatant of SP6391 showed a loss of 22- to 23-kDa proteins and the appearance of a 40-kDa protein corresponding to SspB. Although the 40-kDa SspB protein could degrade denatured collagen, our data establish that this is a precursor form which is normally processed by SspA to form a mature cysteine protease. Culture supernatant of SP6391 also showed a new 42-kDa glucosaminidase and enhanced glucosaminidase activity in the 29 to 32 kDa range. Although nonpolar inactivation of sspA exerted a pleiotropic effect, S. aureus SP6391 exhibited enhanced virulence in a tissue abscess infection model relative to RN6390. Therefore, we conclude that SspA is required for maturation of SspB and plays a role in controlling autolytic activity but does not by itself exert a significant contribution to the development of tissue abscess infections. (+info)Increasing the thermal stability of euphauserase. A cold-active and multifunctional serine protease from Antarctic krill. (7/238)
A molecular model of Antarctic krill euphauserase based on the known crystal structure of its fiddler crab analog, collagenase I, indicates that the core structure of these enzymes is almost identical. Euphauserase is a cold-active and thermally sensitive enzyme with a high affinity for Lys, Arg and large hydrophobic amino acids. Residue Phe137 in euphauserase, localized in loop D (autolysis loop), is highly exposed on the surface of the molecule. Therefore, it appeared to be an easy target for autolysis. The broadly specific euphauserase has a low affinity for negatively charged residues. In order to increase the stability of the enzyme, two mutants were created in which residue Phe137 was replaced by a Glu and an Asp residue. Both mutations resulted in increased stability of the recombinant euphauserase towards thermal inactivation. (+info)The AbcA transporter of Staphylococcus aureus affects cell autolysis. (8/238)
Increased production of penicillin-binding protein PBP 4 is known to increase peptidoglycan cross-linking and contributes to methicillin resistance in Staphylococcus aureus. The pbp4 gene shares a 400-nucleotide intercistronic region with the divergently transcribed abcA gene, encoding an ATP-binding cassette transporter of unknown function. Our study revealed that methicillin stimulated abcA transcription but had no effects on pbp4 transcription. Analysis of abcA expression in mutants defective for global regulators showed that abcA is under the control of agr. Insertional inactivation of abcA by an erythromycin resistance determinant did not influence pbp4 transcription, nor did it alter resistance to methicillin and other cell wall-directed antibiotics. However, abcA mutants showed spontaneous partial lysis on plates containing subinhibitory concentrations of methicillin due to increased spontaneous autolysis. Since the autolytic zymograms of cell extracts were identical in mutants and parental strains, we postulate an indirect role of AbcA in control of autolytic activities and in protection of the cells against methicillin. (+info)
Global Yeasts, Yeast Extracts and Autolysates Market Size, Share,... - Stratistics Market Research Consulting
Trypsin Autolysis Products
ADAM19 autolysis is activated by LPS and promotes non-classical secretion of cysteine-rich protein 2 - Fingerprint -...
XCP1 - Cysteine protease XCP1 precursor - Arabidopsis thaliana (Mouse-ear cress) - XCP1 gene & protein
University of Birmingham research gateway
Autolysis | Harvard Catalyst Profiles | Harvard Catalyst
Herstellung von hohlen mineralischen Strukturen auf der Basis von Hefezellen - University of Regensburg Publication Server
Words starting with A (page 114)
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Frontiers | Release of Mannoproteins during Saccharomyces cerevisiae Autolysis Induced by Pulsed Electric Field | Microbiology
The reason of beer yeast autolysis Suppliers - NINGBO ZHONGRU MACHINERY CO., LTD
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SIKLUS HAID
SIKLUS HAID: Oktober 2007
Health blindness
Isolation and characterization of a possible native cucumisin from developing melon fruits and its limited autolysis to...
Cadaver - Wikipedia
The atlA Operon of Streptococcus mutans: Role in Autolysin Maturation and Cell Surface Biogenesis | Journal of Bacteriology
Re: Is Jucice fasting is starvation? (Fasting: Juice Fasting Support) 5/16/2011 1812438
Autolytic synonyms, autolytic antonyms - FreeThesaurus.com
Combination Chemotherapy Followed by Peripheral Stem Cell Transplantation in Treating Children With Recurrent or Refractory...
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Patent US5720777 - Biological material pre-fixation treatment - Google Patents
Cell lysis synonyms, Cell lysis antonyms - FreeThesaurus.com
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Inhibition of an Autolysin Together with PBP3 Causes the Formation of Bulges: Identification of the Soluble Lytic...
Purification and Properties of the Cathepsin D Type Proteinase from Beef and Rabbit Lung and Its Identification in Macrophages ...
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Histonet] intestines fixation
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Controlled autolysis facilitates the polyhydroxyalkanoate recovery in Pseudomonas putida KT2440 - Martínez - 2011 - Microbial...
A Different Perspective on Macroscopic Sampling of Cholecystectomy Specimens
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LARGE PLASMIDS
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Methods For Inducing Autolysis In Infectious Bacteria - Patent application
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Author: Hosseini, Ebrahim / Journal: Journal of functional foods / Subject: 3 selected / Text Availability: Citation in PubAg...
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Lester Dragstedt
Effect of liver autolysis in vivo. Proc. Soc. Exp. Biol. Med., 26:304-5. With J. C. Ellis. Fatal effect of total loss of ... Liver autolysis in vivo. Arch. Surg., 20:8-16. With M. L. Montgomery, W. B. Matthews, and J. C. Ellis. Fatal effect of the ...
Traditional method
"Autolysis - and its effects on Champagne". Champagne Gallery. Archived from the original on 17 May 2016. Retrieved 17 May 2016 ...
Decomposition
... begins at the moment of death, caused by two factors: 1.) autolysis, the breaking down of tissues by the body's ... Visible changes caused by decomposition are limited during the fresh stage, although autolysis may cause blisters to appear at ... The general stages of decomposition are coupled with two stages of chemical decomposition: autolysis and putrefaction. These ...
GC-content
Levin RE, Van Sickle C (1976). "Autolysis of high-GC isolates of Pseudomonas putrefaciens". Antonie van Leeuwenhoek. 42 (1-2): ... it has been observed that at least some species of bacteria with DNA of high GC-content undergo autolysis more readily, thereby ...
Fixation (histology)
Heat denatures the proteolytic enzyme and prevents autolysis. Heat fixation cannot be used in the capsular stain method as heat ... One reason is to kill the tissue so that postmortem decay (autolysis and putrefaction) is prevented. Fixation preserves ... fixation is the preservation of biological tissues from decay due to autolysis or putrefaction. It terminates any ongoing ...
Lepidium coronopus
Coronopus squamatus) based on its volatile glucosinolate autolysis products". Biochemical Systematics and Ecology. 36 (10): 807 ...
Marmite
... this triggers autolysis, during which the yeast self-destructs. The dying yeast cells are then heated to complete their ...
Protease
Some proteases are less active after autolysis (e.g. TEV protease) whilst others are more active (e.g. trypsinogen). Proteases ...
Pxr sRNA
Wireman JW, Dworkin M (February 1977). "Developmentally induced autolysis during fruiting body formation by Myxococcus xanthus ...
Phi11 holin family
Martínez, Virginia; García, Pedro; García, José Luis; Prieto, María Auxiliadora (2011-07-01). "Controlled autolysis facilitates ...
Botrytis allii
Cell Wall Plant Polysaccharide-degrading Enzymes in Autolysis of Botrytis Cinerea. Science Direct, July 1980. Web. 14 Nov. 2016 ...
Propionibacterium freudenreichii
The autolysis of P. freudenreichii has been suggested to contribute further to the flavor of the Emmental cheese. The ... Ostlie H (1995). "Autolysis of Dairy Propionibacteria: Growth Studies, Peptidase Activities, and Proline Production". Journal ... conditions leading to the autolysis of this bacterium are not well known. Propionibacterium freudenreichii was first discovered ...
Autophagy
They called this autolysis after Christian de Duve and Alex B. Novikoff. However Porter and Ashford wrongly interpreted their ...
Horseradish
Cole, Rosemary A. (1976). "Isothiocyanates, nitriles and thiocyanates as products of autolysis of glucosinolates in Cruciferae ...
Erysimum cheiranthoides
Cole, Rosemary A. (1976). "Isothiocyanates, nitriles and thiocyanates as products of autolysis of glucosinolates in Cruciferae ...
Ficain
Zare H, Moosavi-Movahedi AA, Salami M, Mirzaei M, Saboury AA, Sheibani N (March 2013). "Purification and autolysis of the ficin ...
Calpain-2
Chou JS, Impens F, Gevaert K, Davies PL (July 2011). "m-Calpain activation in vitro does not require autolysis or subunit ...
Insecticide
Cole Rosemary A (1976). "Isothiocyanates, nitriles and thiocyanates as products of autolysis of glucosinolates in Cruciferae". ...
Belinda Ferrari
Ecological Advantages of Autolysis during the Development and Dispersal of Pseudoalteromonas tunicata Biofilms. Applied and ...
Chemical process of decomposition
Autolysis and putrefaction also play major roles in the disintegration of cells and tissues. The human body is composed of ...
Endomembrane system
The last function of a lysosome is to digest the cell itself through autolysis. The spitzenkörper is a component of the ...
John Torrington
... significant cell autolysis had occurred, and cell definition was very poor. His brain was almost completely gone, leaving only ...
Pseudomonas aeruginosa hol holin family
The hol-lys gene system therefore is believed to constitute a chromosomally-encoded autolysis system. The reaction probably ...
Hemicellulose
The sodium bicarbonate inhibits the autolysis of acetyl groups as well as inhibiting glycosyl bonds. Depending on the ...
Cia-dependent small RNAs
Two of these RNAs, csRNA4 and csRNA5, have been shown to affect stationary-phase autolysis. Bacterial small RNA Halfmann, A; ...
Dissolved organic carbon
Phytoplankton can also produce DOC by autolysis during physiological stress situations e.g., nutrient limitation. Other studies ...
Elcatonin
It inhibits the absorption and autolysis of bones and thus leads to lowering of blood calcium. It inhibits bone salts from ...
Chitinase
"Autolysis and aging of Penicillium chrysogenum cultures under carbon starvation: Chitinase production and antifungal effect of ...
Hemolysin
... leading them to autolysis and death. Visualization of hemolysis (UK: haemolysis) of red blood cells in agar plates facilitates ...
Vegemite
Callister used autolysis to break down the yeast cells from waste obtained from the Carlton & United brewery. Concentrating the ...
Autolysis - Wikipedia
Autolysis may refer to: Autolysis (biology), the destruction (or lysis) of a cell by its own enzymes Autocatalysis, in ... in the production of commercial extract This disambiguation page lists articles associated with the title Autolysis. If an ... or catalyzes its own transformation into another compound Autolysis (alcohol fermentation), the complex chemical reactions that ...
XJb Autolysis Glycerol Stock | ZYMO RESEARCH
... and controlled autolysis of E. coli for protein expression and purification. ... XJb Autolysis Glycerol Stock, 1 ml 500x L-Arabinose 1 ml. $119.90 - + ... Autolysis. XJb lysis efficiency is 10-20 % lower than XJa. For optimal lysis, more care needs to be taken when selecting the ... The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). It can be ...
Innovative methods of extracting mannoproteins from lees and accelerating autolysis - Infowine: Internet journal of Viticulture...
Fatal Foodborne Clostridium perfringens Illness at a State Psychiatric Hospital - Louisiana, 2010
S-EPMC6264171 - Transcriptional and functional analysis shows sodium houttuyfonate-mediated inhibition of autolysis in...
Triton X-100-induced autolysis was significantly decreased by SH in S. aureus ATCC 25923, and quantitative bacteriolytic assays ... which suggested that SH may impede biofilm formation by reducing the expression of cidA to inhibit autolysis and eDNA release ... In contrast, the autolysis-deficient phenotype of 14-4 was correlated with both increased expression of negative autolysis ... The inhibition of autolysis by MOL was evaluated using quantitative bacteriolytic assays and zymographic analysis, and ...
Redbook 2000: IV.C.3.a. Short-Term Toxicity Studies with Rodents | FDA
... of animals and tissues or organs lost to a study because of autolysis. Autolysis in excess of this standard may be cause to ... Necropsy should be performed soon after an animal is sacrificed or found dead, so that loss of tissues due to autolysis is ... K. Autolysis:. Adequate animal husbandry practices should result in considerably less than 10% ... the animal should be refrigerated at a temperature that is low enough to prevent autolysis but not so low as to cause cell ...
Chalazion Treatment & Management: Approach Considerations, Conservative Measures, Pharmacologic Therapy
E. coli 'Auto-Induction/Auto-Lysis' + mCherry Positive control plasmid
... strain that has been engineered for protein autoinduction resulting in high titers while also providing cell autolysis and ... Product Description Rokes Autoinduction/Autolysis E. coli strain is a proprietary bacterial ... E. coli "Auto-Induction/Auto-Lysis" + mCherry Positive control plasmid. RSA-001 Regular price $149.00 USD ... Rokes Autoinduction/Autolysis E. coli strain is a proprietary bacterial strain that has been engineered for protein ...
Can the development and autolysis of lactic acid bacteria influence the cheese volatile fraction? The case of Grana Padano
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Leesapo
Pressure Ulcers, A Clinical Review - Rehab Management
Promotes autolysis and a moist wound environment; Aids in pain control and decreased inflammation; Good for Stages II to IV ... Promotes autolysis; Suitable for superficial and partial thickness wounds with minimum to moderate exudate ... Promotes autolysis; Suitable for Stage I and II ulcers; Used for noninfected wounds ...
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Difference between revisions of "Streptococcus pneumoniae: Meningitis" - microbewiki
Autolysis is one pneumococcal process that can initiate host defense. Autolysis involves the bacteria digesting itself by means ... Autolysis of the pneumococcus (as well as antibiotic treatment) leads to the release of subcapsular bacterial products that are ... pneumoniae upon autolysis. Additionally, products from pneumococcal cell walls can trigger an inflammatory response in a host ( ...
Self-digestionBacterialTissueAroma and flavourTissuesFermentationSourdoughYeast cellsToastyTemperatureProcessesInhibitionSaccharomycesEnzymaticHydrolysisPalateSubtleLeesCellsBreakdownOptimizeOxidationPoiseTrypsinFlavoursMechanismsStaphylococcusBacteriaWoundNosePutrefactionStrainPreserveProductionPhaseCell wallLongerMinutesPresentLoopMethodTimeRole
Self-digestion4
- Research shows that human decomposition begins just a few minutes after death via a process of self-digestion known as autolysis, with temperature, humidity, rain and the sun, among other factors, playing an important role in the process. (co.zw)
- The onset is governed by a process called autolysis - or self-digestion. (uaf.edu)
- After a few minutes, autolysis , or self-digestion, sets the stage for decomposition. (myiota.life)
- Decomposition begins several minutes after death with a process called autolysis, or self-digestion. (garrilas.com)
Bacterial5
- Roke's Autoinduction/Autolysis E. coli strain is a proprietary bacterial strain that has been engineered for protein autoinduction resulting in high titers while also providing cell autolysis and nucleotide autohydrolysis capabilities to facilitate routine downstream workflows. (rokebio.com)
- Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis. (google.com.co)
- Over time, meat decays because of two major factors: bacterial growth and autolysis. (oodmag.com)
- At the same time as bacterial activity is taking place, chemical changes within the meat bring about the release of cellular enzymes that initiate the breakdown of cells and tissues, a process called autolysis that starts immediately after death. (oodmag.com)
- In the bush in warmer conditions, we don't have the luxury of hanging meat under ideal conditions, and warm weather can quickly bring the onset of bacterial growth and autolysis, leading to fast decay and rot. (oodmag.com)
Tissue4
- In addition, 2 addi- in brain tissue suggests that USUV tissue samples was not successful be- tional unique amino acid substitutions might have a higher tropism for the cause of autolysis. (cdc.gov)
- These dressings maintain a moist heallng environment, promoting formation of Granylation Tissue and autolysis of necrotic tissue. (mountainside-medical.com)
- The buffer prevents autolysis that occurs under acid conditions and also prevents the development of coloration of the tissue caused by formalin pigment. (nationaldiagnostics.com)
- Autolysis uses the body's own enzymes and moisture to re-hydrate, soften and finally liquefy hard eschar, necrotic tissue and slough. (enluxtra.com)
Aroma and flavour2
- So different on the nose, a definite yeasty autolysis, such a pleasing breadth and depth of aroma and flavour, very fine mousse and a delicious length and balance. (wine-pages.com)
- Ask a wine maker from the Champagne region about the input to aroma and flavour of yeast autolysis and you will hear that it's completely misunderstood and grapes are what the flavour is all about. (chrisshanahan.com)
Tissues2
- Fetal death and ensuing autolysis soften the tissues. (blogspot.com)
- When tissues are removed from the body, the process of autolysis occurs rapidly. (ucsd.edu)
Fermentation4
- Flavors that can also be provided by yeast extracts, if the selection, fermentation and yeast autolysis are well controlled. (lesaffre.com)
- During this aging process, the bottles are kept "en rima", or in a horizontal position, and are left to rest while they undergo a second fermentation. (cava.wine)
- the fermentation in oak barrels is followed in the spring by the "en tirage" process, with the start of a slow second fermentation in bottles, according to the traditional Franciacorta method. (riccicurbastro.it)
- I did the autolysis rest and the double fermentation thing. (kitchensaremonkeybusiness.com)
Sourdough2
- With 36 months of autolysis, it has sourdough, red berry, candy floss and sherbet flavours and well-integrated 7.4 grams of dosage. (kysela.com)
- The best way to make dough with Sourdough is using the Autolysis technique. (biancolievito.com)
Yeast cells3
- After the autolysis the insoluble part such as yeast cells, is separated from the water-soluble components by filtration. (vaisala.com)
- and secondly there is a further overlay caused by contact of the wine with the expired yeast cells as they undergo an enzymatic breakdown (autolysis). (chrisshanahan.com)
- Autolysis is the technical term for yeast cells breaking down in the wine when the Champagne is 'en lees' . (sipchampagnes.com)
Toasty2
- Lively mousse on a tender palate bubbles with toasty, nutty autolysis. (wine.com)
- It's a malty, comparatively dry style with lots of reserve wine richness, flavours of raspberry and milk chocolate, a hint of toasty autolysis and a creamy, well-balanced mid-palate. (timatkin.com)
Temperature2
- This was done by comparing the optimum values of operating parameters such as the yeast concentration, processing temperature and the isolation time of each of the isolation methods used (Autolysis, Ultrasonic and Homogenizer). (scialert.net)
- Unlike Ultrasonic and Homogenizer methods, the temperature for Autolysis method could be maintained within 1 h while for Homogenizer and Ultrasonic methods the temperature could only be maintained within 30 and 15 sec, respectively. (scialert.net)
Processes3
- Maturation on lees therefore combines both these processes - yeast autolysis and slow oxidation - which, together, will continue to hone the style of the wine, gracing it with so-called tertiary aromas of maturity and fullness. (champagne.fr)
- Optimizing production processes (autolysis, microfiltration, drying, etc.) in general in conjunction with the group's industrial management. (lesaffre.com)
- 2002 ) from filamentous fungi such as Aspergillus and Mucor species are suggested to have roles in processes such as hyphal autolysis, growth and branching. (springeropen.com)
Inhibition3
- Transcriptional and functional analysis shows sodium houttuyfonate-mediated inhibition of autolysis in Staphylococcus aureus. (omicsdi.org)
- Transcriptional and functional analysis of the effects of magnolol: inhibition of autolysis and biofilms in Staphylococcus aureus. (omicsdi.org)
- The inhibition of autolysis by MOL was evaluated using quantitative bacteriolytic assays and zymographic analysis, and antibiofilm activity assays and confocal laser scanning microscopy were used to elucidate the inhibition of biofilm formation caused by MOL in 20 clinical isolates or standard strains. (omicsdi.org)
Saccharomyces3
- RumenYeast is a pure yeast from Saccharomyces cerevisiae that goes through the autolysis process in which the end product consists of vitamins, peptides, free amino acids, and functional carbohydrates, such as MOS and β-glucans. (allaboutfeed.net)
- The present work had for objective to optimize the autolysis of fresh brewery's yeast (Saccharomyces cerevisiae), aiming the maximum extraction of ribonucleic acid of biomass in the yeast extract production. (unesp.br)
- Our yeast Extracts are manufactured by autolysis of a selected strain of Saccharomyces cerevisiae. (organotechnie.com)
Enzymatic1
- Moreover, these IMERs enable less enzymatic autolysis, and the online in-flow digestion breaks the analysis time down to a few minutes or seconds compared to the time-consuming offline workflow that often requires overnight digestion (2-8,10-19). (chromatographyonline.com)
Hydrolysis1
- Wang, R., and Lynch, MD., Improved two‐stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease. (rokebio.com)
Palate2
- The palate brings substance to the slender but concentrated palate, and the lemony overtones are now enriched with buttery, lemony brioche while the fine mousse underlines the harmonious balance between ripe lemon freshness, slender poise, statuesque backbone and subtle autolysis. (wine.com)
- Lovely autolysis and a palate that excites throughout. (cluboenologique.com)
Subtle1
- The role of autolysis, they say is subtle and part of a larger comlex. (chrisshanahan.com)
Lees3
- The ageing of a sparkling wine during production 'en lees' to allow the prized flavour of autolysed yeast to develop in the wine. (sipchampagnes.com)
- Aged on the lees for at least 36 months before release, this wine is expressive and complex, with notes of fruits and autolysis balanced by mineral notes and refreshing acidity. (thesavorygrape.com)
- Also during this time, the wine's lees (yeast sediment) releases flavours in a process called autolysis. (thetaste.ie)
Cells3
- Triton X-100-induced autolysis was significantly decreased by SH in S. aureus ATCC 25923, and quantitative bacteriolytic assays and zymographic analysis demonstrated SH-mediated reduction of extracellular murein hydrolase activity in these cells. (omicsdi.org)
- In the process of autolysis the cells will consume themselves by using enzymes from the same cells. (vaisala.com)
- destruction of worn cells parts ('autolysis') and foreign particles? (studystack.com)
Breakdown1
- Autolysis is the breakdown of the body by endogenous substances. (medscape.com)
Optimize1
- Meat-processing plants routinely take advantage of autolysis, a carefully controlled process called dry aging, to optimize meat tenderness. (oodmag.com)
Oxidation1
- Andrea Dal Cin, group technical director for Masi, is in charge of the production process here and draws on Masi's extensive experience to continuously improve the wines, one of the major programmes at the moment is experimenting with yeasts that will increase production of amino acids, to induce autolysis very quickly, thus protecting the wines from oxidation which in turn will minimise the use of sulphur. (wine-pages.com)
Poise1
- Confidently presented with complexity and poise, Altair wears a delicate sheen of savoury autolysis with almonds, baked pastry, poached pear, bright red berries aromas. (campaign-archive.com)
Trypsin2
- Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at -20°C. The presence of Ca 2+ will also diminish the self-autolysis of trypsin and maintain its stability in solution. (sigmaaldrich.com)
- Worthington produces a chemically modified SequENZ® Trypsin with significantly reduced autolysis when used under extensive protein digests, along with specialty packaged trypsin, chymotrypsin, Endo-Arg-C and Endo-GLU-C for proteomics and protein sequencing applications. (worthington-biochem.com)
Flavours2
- Still youthful by the autolysis-rich standards of some Colmant bubblies, this is a structured cuvée of Pinot Noir with 48% Chardonnay, showing raspberry, malt and citrus flavours, leesy underlying texture and a chalky flourish. (kysela.com)
- The ripe fruit flavours of white stonefruit and shortbread enriched with yeast autolysis and brioche. (silverstreamwines.com)
Mechanisms3
- Magnolol (MOL) shows inhibitory activity against S. aureus biofilms and Triton X-100-induced autolysis in vitro, although there are no data regarding the molecular mechanisms of MOL action in bacteria. (omicsdi.org)
- The data suggested the involvement of two novel extracellular serine proteases, nodulin-like proteins and an Arabidopsis thaliana OPEN STOMATA 1 ( AtOST1 ) homolog in signaling fiber-cell death, as well as mechanisms responsible for hormonal control, nutrient remobilization, regulation of vacuolar integrity and autolysis of the dying fibers. (biomedcentral.com)
- The complete digestion of a cell by its own mechanisms is called autolysis, and is a type of apoptosis (cell suicide). (biology-questions-and-answers.com)
Staphylococcus1
- Qamar, A. and Golemi-Kotra, D. Dual roles of FmtA in Staphylococcus aureus cell wall biosynthesis and autolysis. (enzyme-database.org)
Bacteria2
- Can the development and autolysis of lactic acid bacteria influence the cheese volatile fraction? (unipr.it)
- 3 . In order to achieve the best healing outcome, the liquefied products of autolysis, as well as harmful bacteria, disintegrated biofilms, excessive salts, toxins, and other corrosive pro-inflammatory components of chronic wound exudate, must be continuously and non-traumatically evacuated from the wound . (enluxtra.com)
Wound1
- Highly absorbent inner layer effectively removes exudates from the wound and facilitates granulation, epithelization, and autolysis. (dentaldistrictsupply.com)
Nose1
- Bread autolysis dominant nose. (ozwinereview.com)
Putrefaction1
- In most circumstances, autolysis and putrefaction occur in tandem. (medscape.com)
Strain1
- The growth of S. aureus strain ATCC 25923 in the presence of MOL dose-dependently led to decreases in Triton X-100-induced autolysis, extracellular murein hydrolase activity, and the amount of extracellular DNA (eDNA). (omicsdi.org)
Preserve1
- The autolysis is carefully controlled to preserve the naturally occurring B-complex vitamins. (primelabmed.com)
Production3
- The step down arrangement from best to worst for lab scale production was Autolysis>Homogenizer>Ultrasonic method. (scialert.net)
- Autolysis was the best but the isolation time is longer compared to the other so that in large scale production, Homogenizer seems to be the best method due to the economical perspective. (scialert.net)
- 1973. Autolysis and Chitinase Production in Cultures of Verticillium alboatrum. (ugm.ac.id)
Phase5
- Anti-biofilm assay showed that SH is poorly active against S. aureus grown in biofilm cultures, whereas SH diminished the amounts of extracellular DNA (eDNA) of S. aureus in a dose-dependent manner, which suggested that SH may impede biofilm formation by reducing the expression of cidA to inhibit autolysis and eDNA release in the early phase. (omicsdi.org)
- The final phase in maturation of both vessel elements and fibers is cell death and autolysis of the cell contents. (biomedcentral.com)
- In BHI + FCS, 106.66 shows the typical exponential phase followed by a peak OD 450nm followed by an autolysis phase. (biomedcentral.com)
- In hCSF, 106.66 lacked the typical exponential phase, peak OD 450nm and the following autolysis phase. (biomedcentral.com)
- But do I also want a longer autolysis phase? (chimpanzeeteaparty.com)
Cell wall1
- The process of autolysis in combination with 9.8% of NaCl, the RNA extraction yield in 24 hours was of 89.7%, with a yield of 51.3% in extract mass with 57.9% of protein and, 48.7% of dehydrated cell wall with 21.7% of protein. (unesp.br)
Longer1
- The longer this process of 'autolysis' persists the better, improving mouthfeel and longevity, and adding biscuity, bready nuances to the flavour. (tysonstelzer.com)
Minutes1
- The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). (zymoresearch.com)
Present1
- When glucose is added to the growth media, it inhibits the induction of the autolysis genes when it is present in the media. (zymoresearch.com)
Loop2
- A 1.9-Angstroms resolution crystal structure of free thrombin grown in the presence of high salt (400 mm KCl) shows two molecules in the asymmetric unit, one of which assumes an unprecedented conformation with the autolysis loop shifted 20 Angstroms away from its canonical position, the 220-loop entirely disordered, and the RGD sequence exposed to the solvent. (rcsb.org)
- A study by Bonde et al indicated that the factor X autolysis loop takes part in all elements of factor X regulation. (medscape.com)
Method2
- I was trying to explain the autolyse method to my sister, who's a pathologist, and her eyes lit up: "Autolysis! (kingarthurbaking.com)
- Among the three methods, Autolysis method produced the highest GSH concentration of 49.263 μmol mL -1 (15.14 g L -1 ) followed by Homogenizer method (18.33 μmol mL -1 or 5.63 g L -1 ) which is slightly higher than that of the Ultrasonic method (18.13 μmol mL -1 or 5.57 g L -1 ). (scialert.net)
Time2
- Will this be a problem as time goes by (autolysis? (homebrewersassociation.org)
- When the time came to rack the vineyard designate to tank, I decided to satisfy a longtime curiosity by blending wine from these two new barrels into an older barrel for further surlie aging where yeast autolysis and further harmonization could occur. (scherrerwinery.com)
Role2
- They are frequently nicknamed "suicide-bags" or "suicide-sacs" by cell biologists due to their role in autolysis. (phys.org)
- Pathological-anatomical autopsy is the gold standard for determining of foetal abnormalities, but in some cases its role is limited (pathology of central nervous system, in particular, in case of ventricular dilatation or developed autolysis). (nel.edu)