Receptor-Like Protein Tyrosine Phosphatases, Class 8
A pyridoxal-phosphate protein that catalyzes the alpha-decarboxylation of L-glutamic acid to form gamma-aminobutyric acid and carbon dioxide. The enzyme is found in bacteria and in invertebrate and vertebrate nervous systems. It is the rate-limiting enzyme in determining GAMMA-AMINOBUTYRIC ACID levels in normal nervous tissues. The brain enzyme also acts on L-cysteate, L-cysteine sulfinate, and L-aspartate. EC 18.104.22.168.
Lupus Erythematosus, Systemic
A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.
RNA, Small Cytoplasmic
Diabetes Mellitus, Type 1
A subtype of DIABETES MELLITUS that is characterized by INSULIN deficiency. It is manifested by the sudden onset of severe HYPERGLYCEMIA, rapid progression to DIABETIC KETOACIDOSIS, and DEATH unless treated with insulin. The disease may occur at any age, but is most common in childhood or adolescence.
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Inflammation of a TESTIS. It has many features of EPIDIDYMITIS, such as swollen SCROTUM; PAIN; PYURIA; and FEVER. It is usually related to infections in the URINARY TRACT, which likely spread to the EPIDIDYMIS and then the TESTIS through either the VAS DEFERENS or the lymphatics of the SPERMATIC CORD.
CapZ Actin Capping Protein
Liver Cirrhosis, Biliary
FIBROSIS of the hepatic parenchyma due to obstruction of BILE flow (CHOLESTASIS) in the intrahepatic or extrahepatic bile ducts (BILE DUCTS, INTRAHEPATIC; BILE DUCTS, EXTRAHEPATIC). Primary biliary cirrhosis involves the destruction of small intra-hepatic bile ducts and bile secretion. Secondary biliary cirrhosis is produced by prolonged obstruction of large intrahepatic or extrahepatic bile ducts from a variety of causes.
snRNP Core Proteins
Protein Tyrosine Phosphatase, Non-Receptor Type 1
A subtype of non-receptor protein tyrosine phosphatases that includes two distinctive targeting motifs; an N-terminal motif specific for the INSULIN RECEPTOR, and a C-terminal motif specific for the SH3 domain containing proteins. This subtype includes a hydrophobic domain which localizes it to the ENDOPLASMIC RETICULUM.
Mice, Inbred NOD
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Islets of Langerhans
Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.
A pancreatic polypeptide of about 110 amino acids, depending on the species, that is the precursor of insulin. Proinsulin, produced by the PANCREATIC BETA CELLS, is comprised sequentially of the N-terminal B-chain, the proteolytically removable connecting C-peptide, and the C-terminal A-chain. It also contains three disulfide bonds, two between A-chain and B-chain. After cleavage at two locations, insulin and C-peptide are the secreted products. Intact proinsulin with low bioactivity also is secreted in small amounts.
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Protein Array Analysis
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Chronic inflammatory and autoimmune disease in which the salivary and lacrimal glands undergo progressive destruction by lymphocytes and plasma cells resulting in decreased production of saliva and tears. The primary form, often called sicca syndrome, involves both KERATOCONJUNCTIVITIS SICCA and XEROSTOMIA. The secondary form includes, in addition, the presence of a connective tissue disease, usually rheumatoid arthritis.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.
Autoimmune diseases affecting multiple endocrine organs. Type I is characterized by childhood onset and chronic mucocutaneous candidiasis (CANDIDIASIS, CHRONIC MUCOCUTANEOUS), while type II exhibits any combination of adrenal insufficiency (ADDISON'S DISEASE), lymphocytic thyroiditis (THYROIDITIS, AUTOIMMUNE;), HYPOPARATHYROIDISM; and gonadal failure. In both types organ-specific ANTIBODIES against a variety of ENDOCRINE GLANDS have been detected. The type II syndrome differs from type I in that it is associated with HLA-A1 and B8 haplotypes, onset is usually in adulthood, and candidiasis is not present.
Myelin Basic Protein
A chronic multi-system disorder of CONNECTIVE TISSUE. It is characterized by SCLEROSIS in the SKIN, the LUNGS, the HEART, the GASTROINTESTINAL TRACT, the KIDNEYS, and the MUSCULOSKELETAL SYSTEM. Other important features include diseased small BLOOD VESSELS and AUTOANTIBODIES. The disorder is named for its most prominent feature (hard skin), and classified into subsets by the extent of skin thickening: LIMITED SCLERODERMA and DIFFUSE SCLERODERMA.
The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)
Electrophoresis, Gel, Two-Dimensional
Centromere Protein B
A disorder consisting of areas of macular depigmentation, commonly on extensor aspects of extremities, on the face or neck, and in skin folds. Age of onset is often in young adulthood and the condition tends to progress gradually with lesions enlarging and extending until a quiescent state is reached.
Inflammatory disease of the THYROID GLAND due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-CELLS and thyroid AUTOANTIBODIES. The clinical signs can range from HYPOTHYROIDISM to THYROTOXICOSIS depending on the type of autoimmune thyroiditis.
Ribonucleoproteins, Small Nuclear
Ribonucleoprotein, U1 Small Nuclear
A nuclear RNA-protein complex that plays a role in RNA processing. In the nucleoplasm, the U1 snRNP along with other small nuclear ribonucleoproteins (U2, U4-U6, and U5) assemble into SPLICEOSOMES that remove introns from pre-mRNA by splicing. The U1 snRNA forms base pairs with conserved sequence motifs at the 5'-splice site and recognizes both the 5'- and 3'-splice sites and may have a fundamental role in aligning the two sites for the splicing reaction.
Anemia, Hemolytic, Autoimmune
Mice, Inbred MRL lpr
A mouse substrain that is genetically predisposed to the development of systemic lupus erythematosus-like syndrome, which has been found to be clinically similar to the human disease. It has been determined that this mouse strain carries a mutation in the fas gene. Also, the MRL/lpr is a useful model to study behavioral and cognitive deficits found in autoimmune diseases and the efficacy of immunosuppressive agents.
Cell surface proteins that bind pituitary THYROTROPIN (also named thyroid stimulating hormone or TSH) and trigger intracellular changes of the target cells. TSH receptors are present in the nervous system and on target cells in the thyroid gland. Autoantibodies to TSH receptors are implicated in thyroid diseases such as GRAVES DISEASE and Hashimoto disease (THYROIDITIS, AUTOIMMUNE).
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Connective Tissue Diseases
Fluorescent Antibody Technique, Indirect
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Diseases characterized by inflammation involving multiple muscles. This may occur as an acute or chronic condition associated with medication toxicity (DRUG TOXICITY); CONNECTIVE TISSUE DISEASES; infections; malignant NEOPLASMS; and other disorders. The term polymyositis is frequently used to refer to a specific clinical entity characterized by subacute or slowly progressing symmetrical weakness primarily affecting the proximal limb and trunk muscles. The illness may occur at any age, but is most frequent in the fourth to sixth decade of life. Weakness of pharyngeal and laryngeal muscles, interstitial lung disease, and inflammation of the myocardium may also occur. Muscle biopsy reveals widespread destruction of segments of muscle fibers and an inflammatory cellular response. (Adams et al., Principles of Neurology, 6th ed, pp1404-9)
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Glomerulonephritis associated with autoimmune disease SYSTEMIC LUPUS ERYTHEMATOSUS. Lupus nephritis is histologically classified into 6 classes: class I - normal glomeruli, class II - pure mesangial alterations, class III - focal segmental glomerulonephritis, class IV - diffuse glomerulonephritis, class V - diffuse membranous glomerulonephritis, and class VI - advanced sclerosing glomerulonephritis (The World Health Organization classification 1982).
Alteration of the immune system or of an immune response by agents that activate or suppress its function. This can include IMMUNIZATION or administration of immunomodulatory drugs. Immunomodulation can also encompass non-therapeutic alteration of the immune system effected by endogenous or exogenous substances.
Mixed Connective Tissue Disease
A syndrome with overlapping clinical features of systemic lupus erythematosus, scleroderma, polymyositis, and Raynaud's phenomenon. The disease is differentially characterized by high serum titers of antibodies to ribonuclease-sensitive extractable (saline soluble) nuclear antigen and a "speckled" epidermal nuclear staining pattern on direct immunofluorescence.
Disease Models, Animal
An autoimmune disorder mainly affecting young adults and characterized by destruction of myelin in the central nervous system. Pathologic findings include multiple sharply demarcated areas of demyelination throughout the white matter of the central nervous system. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia, and bladder dysfunction. The usual pattern is one of recurrent attacks followed by partial recovery (see MULTIPLE SCLEROSIS, RELAPSING-REMITTING), but acute fulminating and chronic progressive forms (see MULTIPLE SCLEROSIS, CHRONIC PROGRESSIVE) also occur. (Adams et al., Principles of Neurology, 6th ed, p903)
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Histocompatibility Antigens Class II
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
Immunoglobulin Variable Region
That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
A subacute or chronic inflammatory disease of muscle and skin, marked by proximal muscle weakness and a characteristic skin rash. The illness occurs with approximately equal frequency in children and adults. The skin lesions usually take the form of a purplish rash (or less often an exfoliative dermatitis) involving the nose, cheeks, forehead, upper trunk, and arms. The disease is associated with a complement mediated intramuscular microangiopathy, leading to loss of capillaries, muscle ischemia, muscle-fiber necrosis, and perifascicular atrophy. The childhood form of this disease tends to evolve into a systemic vasculitis. Dermatomyositis may occur in association with malignant neoplasms. (From Adams et al., Principles of Neurology, 6th ed, pp1405-6)
Receptors, Antigen, B-Cell
A disorder of neuromuscular transmission characterized by weakness of cranial and skeletal muscles. Autoantibodies directed against acetylcholine receptors damage the motor endplate portion of the NEUROMUSCULAR JUNCTION, impairing the transmission of impulses to skeletal muscles. Clinical manifestations may include diplopia, ptosis, and weakness of facial, bulbar, respiratory, and proximal limb muscles. The disease may remain limited to the ocular muscles. THYMOMA is commonly associated with this condition. (Adams et al., Principles of Neurology, 6th ed, p1459)
Encephalomyelitis, Autoimmune, Experimental
An experimental animal model for central nervous system demyelinating disease. Inoculation with a white matter emulsion combined with FREUND'S ADJUVANT, myelin basic protein, or purified central myelin triggers a T cell-mediated immune response directed towards central myelin. The pathologic features are similar to MULTIPLE SCLEROSIS, including perivascular and periventricular foci of inflammation and demyelination. Subpial demyelination underlying meningeal infiltrations also occurs, which is also a feature of ENCEPHALOMYELITIS, ACUTE DISSEMINATED. Passive immunization with T-cells from an afflicted animal to a normal animal also induces this condition. (From Immunol Res 1998;17(1-2):217-27; Raine CS, Textbook of Neuropathology, 2nd ed, p604-5)
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
A class of closely related heterogeneous-nuclear ribonucleoproteins of approximately 34-40 kDa in size. Although they are generally found in the nucleoplasm, they also shuttle between the nucleus and the cytoplasm. Members of this class have been found to have a role in mRNA transport, telomere biogenesis and RNA SPLICING.
Binding Sites, Antibody
Pyruvate Dehydrogenase Complex
A multienzyme complex responsible for the formation of ACETYL COENZYME A from pyruvate. The enzyme components are PYRUVATE DEHYDROGENASE (LIPOAMIDE); dihydrolipoamide acetyltransferase; and LIPOAMIDE DEHYDROGENASE. Pyruvate dehydrogenase complex is subject to three types of control: inhibited by acetyl-CoA and NADH; influenced by the energy state of the cell; and inhibited when a specific serine residue in the pyruvate decarboxylase is phosphorylated by ATP. PYRUVATE DEHYDROGENASE (LIPOAMIDE)-PHOSPHATASE catalyzes reactivation of the complex. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed)
Myelin Proteolipid Protein
A myelin protein that is the major component of the organic solvent extractable lipoprotein complexes of whole brain. It has been the subject of much study because of its unusual physical properties. It remains soluble in chloroform even after essentially all of its bound lipids have been removed. (From Siegel et al., Basic Neurochemistry, 4th ed, p122)
Toll-Like Receptor 7
Toll-Like Receptor 9
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Proteins which bind with RETINOL. The retinol-binding protein found in plasma has an alpha-1 mobility on electrophoresis and a molecular weight of about 21 kDa. The retinol-protein complex (MW=80-90 kDa) circulates in plasma in the form of a protein-protein complex with prealbumin. The retinol-binding protein found in tissue has a molecular weight of 14 kDa and carries retinol as a non-covalently-bound ligand.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Receptors, Antigen, T-Cell
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Explanations for the clinical and microscopic localization of lesions in pemphigus foliaceus and vulgaris. (1/5266)Patients with pemphigus foliaceus (PF) have blisters on skin, but not mucous membranes, whereas patients with pemphigus vulgaris (PV) develop blisters on mucous membranes and/or skin. PF and PV blisters are due to loss of keratinocyte cell-cell adhesion in the superficial and deep epidermis, respectively. PF autoantibodies are directed against desmoglein (Dsg) 1; PV autoantibodies bind Dsg3 or both Dsg3 and Dsg1. In this study, we test the hypothesis that coexpression of Dsg1 and Dsg3 in keratinocytes protects against pathology due to antibody-induced dysfunction of either one alone. Using passive transfer of pemphigus IgG to normal and DSG3(null) neonatal mice, we show that in the areas of epidermis and mucous membrane that coexpress Dsg1 and Dsg3, antibodies against either desmoglein alone do not cause spontaneous blisters, but antibodies against both do. In areas (such as superficial epidermis of normal mice) where Dsg1 without Dsg3 is expressed, anti-Dsg1 antibodies alone can cause blisters. Thus, the anti-desmoglein antibody profiles in pemphigus sera and the normal tissue distributions of Dsg1 and Dsg3 determine the sites of blister formation. These studies suggest that pemphigus autoantibodies inhibit the adhesive function of desmoglein proteins, and demonstrate that either Dsg1 or Dsg3 alone is sufficient to maintain keratinocyte adhesion. (+info)
Herpes virus induced proteasome-dependent degradation of the nuclear bodies-associated PML and Sp100 proteins. (2/5266)The PML protein is associated to nuclear bodies (NBs) whose functions are as yet unknown. PML and two other NBs-associated proteins, Sp100 And ISG20 are directly induced by interferons (IFN). PML and Sp100 proteins are covalently linked to SUMO-1, and ubiquitin-like peptide. PML NBs are disorganized in acute promyelocytic leukemia and during several DNA virus infections. In particular, the HSV-1 ICP0 protein is known to delocalize PML from NBs. Thus, NBs could play an important role in oncogenesis, IFN response and viral infections. Here, we show that HSV-1 induced PML protein degradation without altering its mRNA level. This degradation was time- and multiplicity of infection-dependent. Sp100 protein was also degraded, while another SUMO-1 conjugated protein, RanGAP1 and the IFN-induced protein kinase PKR were not. The proteasome inhibitor MG132 abrogated the HSV-1-induced PML and Sp100 degradation and partially restored their NB-localization. HSV-1 induced PML and Sp100 degradation constitutes a new example of viral inactivation of IFN target gene products. (+info)
The endosome fusion regulator early-endosomal autoantigen 1 (EEA1) is a dimer. (3/5266)EEA1, an early-endosomal protein originally identified as an autoantigen, is essential for endocytic membrane fusion. It interacts with early endosomes via binding to the membrane lipid phosphatidylinositol 3-phosphate (PtdIns3P) and the active form of the small GTPase Rab5. Most of the EEA1 sequence contains heptad repeats characteristic of proteins involved in coiled-coil protein-protein interactions. Here we have investigated the ability of EEA1 to self-interact. Crosslinking of cytosolic and recombinant EEA1 resulted in the disappearance of the 180-kDa monomer in SDS/PAGE and the strong appearance of a approximately 350-kDa crosslinked product. Glycerol gradient centrifugation experiments indicated that native EEA1 had the same hydrodynamic properties as the approximately 350-kDa crosslinked complex. Two-hybrid analysis indicated that N- and C-terminal fragments of EEA1 can interact with themselves, but not with each other, suggesting that EEA1 forms parallel coiled-coil dimers. The ability of the C-terminus of EEA1 to dimerize correlates with its ability to bind to Rab5 and early endosomes, whereas its binding to PtdIns3P is independent of dimerization. These data enable us to propose a model for the quaternary structure of EEA1. (+info)
Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs. (4/5266)The U1, U2, U4/U6, and U5 small nuclear ribonucleoprotein particles (snRNPs) involved in pre-mRNA splicing contain seven Sm proteins (B/B', D1, D2, D3, E, F, and G) in common, which assemble around the Sm site present in four of the major spliceosomal small nuclear RNAs (snRNAs). These proteins share a common sequence motif in two segments, Sm1 and Sm2, separated by a short variable linker. Crystal structures of two Sm protein complexes, D3B and D1D2, show that these proteins have a common fold containing an N-terminal helix followed by a strongly bent five-stranded antiparallel beta sheet, and the D1D2 and D3B dimers superpose closely in their core regions, including the dimer interfaces. The crystal structures suggest that the seven Sm proteins could form a closed ring and the snRNAs may be bound in the positively charged central hole. (+info)
Fine specificity of the autoimmune response to the Ro/SSA and La/SSB ribonucleoproteins. (5/5266)The fine specificity of the Ro and La proteins has been studied by several techniques. In general, there is agreement in a qualitative sense that autoantibodies bind multiple epitopes. For some specific antibody binding, different studies agree quantitatively, for instance, the binding of the carboxyl terminus of 60-kd Ro as described by 2 studies using different techniques and the presence of an epitope within the leucine zipper of 52-kd Ro. In addition, there is general agreement about the location of a prominent epitope at the RRM motif region of the La molecule. On the other hand, the many specific epitope regions of the molecules differ among these studies. These discrepancies are likely the result of using different techniques, sera, and peptide constructs as well as a result of inherent advantages and disadvantages in the individual approaches. Several theories concerning the origin of not only the antibodies, but also the diseases themselves, have been generated from studies of the fine specificity of antibody binding. These include a theory of a primordial foreign antigen for anti-Ro autoimmunity, molecular mimicry with regard to La and CCHB, as well as the association of anti-Ro with HLA. These remain unproven, but are of continuing interest. An explanation for the association of anti-60-kd Ro and anti-52-kd Ro in the sera of patients has sprung from evaluating antibody binding. Data demonstrating multiple epitopes are part of a large body of evidence that strongly suggests an antigen-driven immune response. This means that the autoantigens are directly implicated in initiating and sustaining autoimmunity in their associated diseases. A number of studies have investigated the possibility of differences in the immune response to these antigens in SS and SLE sera. While several differences have been reported, none have been reproduced in a second cohort of patients. Furthermore, none of the reported differences may be sufficiently robust for clinical purposes, such as distinguishing between SS with systemic features and mild SLE, although some might be promising. For instance, in at least 3 groups of SLE patients, no binding of residues spanning amino acids 21-41 of 60-kd Ro has been found. Meanwhile, 1 of those studies found that 41% of sera from patients with primary SS bound the 60-kd Ro peptide 21-41. Perhaps future studies will elaborate a clinical role of such a difference among SS and SLE patients. Study of the epitopes of these autoantigens has, in part, led to a new animal model of anti-Ro and anti-La. Non-autoimmune-prone animals are immunized with proteins or peptides that make up the Ro/La RNP. Such animals develop an autoimmune response to the entire particle, not just the immunogen. This response has been hypothesized to arise from autoreactive B cells. In another, older animal model of disease, the MRL-lpr/lpr mouse, B cells have recently been shown to be required for the generation of abnormal, autoreactive T cells. Thus, there are now powerful data indicating that B cells that produce autoantibodies are directly involved in the pathogenesis of disease above and beyond the formation of immune complexes. Given that the autoreactive B cell is potentially critical to the underlying pathogenesis of disease, then studying these cells will be crucial to further understanding the origin of diseases associated with Ro and La autoimmunity. Hopefully, an increased understanding will eventually lead to improved treatment of patients. Progress in the area of treatment will almost surely be incremental, and studies of the fine specificity of autoantibody binding will be a part of the body of basic knowledge contributing to ultimate advancement. In the future, the animal models will need to be examined with regard to immunology and immunochemistry as well as genetics. The development of these autoantibodies has not been studied extensively because upon presentation to medical care, virtually all patients have a full- (+info)
Goodpasture antigen: expression of the full-length alpha3(IV) chain of collagen IV and localization of epitopes exclusively to the noncollagenous domain. (6/5266)BACKGROUND: Tissue injury in Goodpasture (GP) syndrome (rapidly progressive glomerular nephritis and pulmonary hemorrhage) is mediated by antibasement membrane antibodies that are targeted to the alpha3(IV) chain of type IV collagen, one of five alpha(IV) chains that occur in the glomerular basement membrane. GP antibodies are known to bind epitopes within the carboxyl terminal noncollagenous domain (NC1) of the alpha3(IV) chain, termed the GP autoantigen. Whether epitopes also exist in the 1400-residue collagenous domain is unknown because studies to date have focused solely on the NC1 domain. A knowledge of GP epitopes is important for the understanding of the etiology and pathogenesis of the disease and for the development of therapeutic strategies. METHODS: A cDNA construct was prepared for the full-length human alpha3(IV) chain. The construct was stably transfected into human embryonic kidney 293 cells. The purified full-length r-alpha3(IV) chain was characterized by electrophoresis and electron microscopy. The capacity of this chain for binding of GP antibodies from five patients was compared with that of the human r-alpha3(IV)NC1 domain by competitive enzyme-linked immunosorbent assay. RESULTS: The r-alpha3(IV) chain was secreted from 293 cells as a single polypeptide chain that did not spontaneously undergo assembly into a triple-helical molecule. An analysis of GP-antibody binding to the full-length r-alpha3(IV) chain showed binding exclusively to the globular NC1 domain. CONCLUSION: The full-length human alpha3(IV) chain possesses the capacity to bind GP autoantibodies. The epitope(s) is found exclusively on the nontriple-helical NC1 domain of the alpha3(IV) chain, indicating the presence of specific immunogenic properties. The alpha3(IV) chain alone does not spontaneously undergo assembly into a triple-helical homotrimeric molecule, suggesting that coassembly with either the alpha4(IV) and/or the alpha5(IV) chain may be required for triple-helix formation. (+info)
Identification of a clinically relevant immunodominant region of collagen IV in Goodpasture disease. (7/5266)BACKGROUND: The characteristic feature of Goodpasture disease is the occurrence of an autoantibody response to the noncollagenous domain of the alpha3 chain of type IV collagen [alpha3(IV)NC1] in the alveolar and glomerular basement membrane. These antibodies are associated with the development of a rapidly progressive glomerulonephritis, with or without lung hemorrhage, whereas autoantibodies specific for the other alpha chains of the heterotrimeric type IV collagen probably do not cause disease. In this study, we have investigated whether differences in fine specificity of autoimmune recognition of the alpha3(IV)NC1 correlate with clinical outcome. METHODS: For mapping of antibody binding to type IV collagen, chimeric collagen constructs were generated in which parts of the alpha3(IV)NC1 domain were replaced by the corresponding sequences of homologous nonreactive alpha1(IV). The different recombinant collagen chimeras allowed the analysis of antibody specificities in 77 sera from well-documented patients. RESULTS: One construct that harbors the aminoterminal third of the alpha3(IV)NC1 was recognized by all sera, indicating that it represents the dominant target of the B-cell response in Goodpasture disease. Seventy percent of the samples recognized other parts of the molecule as well. However, only reactivity to the N-terminus of the alpha3(IV)NC1 correlated with prognosis, that is, kidney survival after six months of follow-up. CONCLUSION: The results indicate the crucial importance of antibody recognition of this particular domain for the pathogenesis of Goodpasture disease, thereby opening new avenues for the development of better diagnostic and therapeutic procedures. (+info)
Activated human T cells, B cells, and monocytes produce brain-derived neurotrophic factor in vitro and in inflammatory brain lesions: a neuroprotective role of inflammation? (8/5266)Brain-derived neurotrophic factor (BDNF) has potent effects on neuronal survival and plasticity during development and after injury. In the nervous system, neurons are considered the major cellular source of BDNF. We demonstrate here that in addition, activated human T cells, B cells, and monocytes secrete bioactive BDNF in vitro. Notably, in T helper (Th)1- and Th2-type CD4(+) T cell lines specific for myelin autoantigens such as myelin basic protein or myelin oligodendrocyte glycoprotein, BDNF production is increased upon antigen stimulation. The BDNF secreted by immune cells is bioactive, as it supports neuronal survival in vitro. Using anti-BDNF monoclonal antibody and polyclonal antiserum, BDNF immunoreactivity is demonstrable in inflammatory infiltrates in the brain of patients with acute disseminated encephalitis and multiple sclerosis. The results raise the possibility that in the nervous system, inflammatory infiltrates have a neuroprotective effect, which may limit the success of nonselective immunotherapies. (+info)
Nasp - Nuclear autoantigenic sperm protein - Mus musculus (Mouse) - Nasp gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Depletion of the histone chaperon... preview & related info | Mendeley
(2011) Alekseev et al. Reproductive Biology and Endocrinology. Background: NASP (Nuclear Autoantigenic Sperm Protein) is a histone chaperone that is present in all dividing cells. NASP has two splice variants: tNASP and sNASP. Only cancer, germ, transformed, and embryonic cells have a high level ...
Comment on Identification of novel autoantigens via mass spectroscopy-based antibody-mediated identification of autoantigens ...
Identification of novel autoantigens via mass spectroscopy-based antibody-mediated identification of autoantigens (MS-AMIDA) using immune thrombocytopenic purpura (ITP) as a model ...
Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by...
Fingerprint Dive into the research topics of Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome. Together they form a unique fingerprint. ...
Antibodies to gangliosides and myelin autoantigens are produced in mic by M Schwartz, B A. Sela et al.
Schwartz, M; Sela, B A.; and Eshhar, N, Antibodies to gangliosides and myelin autoantigens are produced in mice following sciatic nerve injury. (1982). Subject Strain Bibliography 1982. 2752 ...
Several scleroderma autoantigens are uniquely fragmente | Open-i
Several scleroderma autoantigens are uniquely fragmented by Fe/ascorbate or Cu/H2O2 oxidation reactions. HeLa lysates were prepared as described in the Materi
Repression of B7.2 on Self-reactive B Cells Is Essential to Prevent Proliferation and Allow Fas-mediated Deletion by CD4+ T...
The findings here demonstrate that selectively restoring B7.2 expression on otherwise self-tolerant B cells is sufficient to switch their fate from peripheral deletion to large-scale proliferation and autoantibody secretion during interactions with specific CD4+ T cells. Of the many early signals and responses to BCR engagement that are repressed or altered in tolerant B cells ((12)-(14)), the data here single out repression of the B7.2 response as being necessary to allow peripheral tolerance by FasL/Fas-mediated clonal abortion. The findings raise interesting questions about how B7.2 switches the outcome of interactions between tolerant B and T cells, and highlight the regulation of B7.2 in B cells as a key process that may be dysregulated by inherited or acquired triggers to systemic autoimmune disease.. Three possible mechanisms may in principle explain how dysregulated expression of B7.2 on tolerant B cells switches the outcome of interactions with autoantigen-specific T cells from deletion ...
Concerted potent humoral immune responses to autoantigens are associat by Taylor Sittler, Jun Zhou et al.
PURPOSE: The therapeutic importance of immune responses against single versus multiple antigens is poorly understood. There also remains insufficient understanding whether responses to one subset of antigens are more significant than another. Autoantibodies are frequent in cancer patients. They can pose no biological significance or lead to debilitating paraneoplastic syndromes. Autoreactivity has been associated with clinical benefits, but the magnitude necessary for meaningful results is unknown. Autologous tumor cells engineered to secrete granulocyte macrophage colony-stimulating factor generate immune infiltrates in preexisting metastases with associated tumor destruction. We sought to identify targets of responses from this vaccination strategy. EXPERIMENTAL DESIGN: Postvaccination sera used in screening a cDNA expression library prepared from a densely infiltrated metastasis of a long-term surviving melanoma patient identified several autoantigens. Additional autoantigens were identified through
Regulatory T cells inhibit stable contacts between CD4+ T cells and dendritic cells in vivo. - Immunology
Regulatory T (T reg) cells exert powerful down-modulatory effects on immune responses, but it is not known how they act in vivo. Using intravital two-photon laser scanning microscopy we determined that, in the absence of T reg cells, the locomotion of autoantigen-specific T cells inside lymph nodes is decreased, and the contacts between T cells and antigen-loaded dendritic cells (DCs) are of longer duration. Thus, T reg cells can exert an early effect on immune responses by attenuating the establishment of stable contacts during priming of naive T cells by DCs.
Autoreactive T cells bypass negative selection and respond to self-antigen stimulation during infection | JEM
Most autoreactive T cells are exposed to self-antigen either in the thymus or the periphery, and high avidity T cells are eliminated in both compartments. In contrast, lower avidity T cells are often found in ongoing autoimmune diseases (Bulek et al., 2012) and in anti-tumor responses (McMahan and Slansky, 2007). Thus, expression of a low avidity TCR, allowing autoreactive T cells to bypass elimination, is a major mechanism by which autoreactive T cells escape tolerance (von Herrath et al., 1994; Nugent et al., 2000; Zehn and Bevan, 2006). However, up to this point, we had limited insight into the phenotypic and functional characteristics of these low avidity self-reactive T cells. Thus, it was unclear how self-antigen recognition in the thymus or periphery impacts the function of these T cells and whether such exposure imprinted mechanisms that restrict their activation. The failure to negatively select low avidity self-reactive T cells stimulated us to study how well these T cells respond to ...
Regulation of cathepsin G reduces the activation of proinsulin-reactive T cells from type 1 diabetes patients.
Autoantigenic peptides resulting from self-proteins such as proinsulin are important players in the development of type 1 diabetes mellitus (T1D). Self-proteins can be processed by cathepsins (Cats) within endocytic compartments and loaded to major h
JCI - Autoreactive T cells in type 1 diabetes
Schematic representation of major autoantigen classes that may be targeted by CD4+ T cells. Similar to CD8+ T cells, these may include native antigens and neoepitopes, which may be formed under conditions of β cell inflammation and stress. β Cells can produce several neoepitopes: for example, peptides originating from alternative splicing, insulin hybrid peptides (HP), and DRiP insulin peptides are reportedly produced in β cells (for DRiPs, the evidence is from studies of cell lines). Many autoantigens are secretory granule proteins, which may be released by β cells and acquired by APCs. Exosomes released by β cells also contain autoantigens. CD4+ T cells may react with antigens captured, processed, and presented by APCs in the pancreatic lymph node and in the islets. The B:9-23 epitope is produced in the secretory granules, captured, and presented by APCs, at least in the NOD mouse. The generation of neoepitopes in β cells further raises the question of whether the previously reported ...
Cell surface expression of the 70-kD component of Ku, a DNA-bi...
Cell surface expression of the 70-kD component of Ku, a DNA-binding nuclear autoantigen.: The Ku complex, a heterodimer of 86- and 70-kD proteins, is a nuclear
RCSB PDB - Protein Feature View - Nuclear autoantigen Sp-100 - H0Y4R8 (H0Y4R8 HUMAN)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
TNO Repository search for: subject:Autoantigen
Tolerance induction to prevent activation of a naïve T-cell repertoire has been well documented in rodents and can be readily achieved by intravenous, oral or intranasal administration of antigen in the absence of adjuvants. In autoimmune diseases such as multiple sclerosis (MS) the presence of an established memory/effector T-cell repertoire against self-antigens is likely to be more relevant than the potential reactivity of naive T cells. Methods to eliminate such an established T-cell response are less well understood. In this study, we explored the effectiveness of intravenous soluble antigen to eliminate a pre-existing T-cell response against αB-crystallin, a candidate autoantigen in MS. We used mice that are deficient for the target antigen. This condition allowed for a vigourous T-cell and antibody response to develop upon immunization, and eliminated all possible endogenous mechanisms of tolerance for αB-crystallin that are found in normal rodents. When applied 3 weeks after priming ...
JCI - Cathepsin S inhibitor prevents autoantigen presentation and autoimmunity
Unique Clinical and Pathological Features in HLA-DRB1*0401-restricted MBP 111-129-specific Humanized TCR Transgenic Mice |...
Clearly, several factors can contribute to the clinical manifestations of EAE in any one particular model, perhaps the two greatest contributors being the genetic background and the immunizing myelin antigen or the target autoantigen. It has been reported that the antigenic epitope specificity of myelin antigens influences the phenotype of EAE and location of the lesions. In Balb/c (IAd, IEd) mice, IAd-restricted MBP 59-76-specific T cells induce classical EAE with characteristic inflammation and demyelination of the CNS (34). In contrast, IEd-restricted MBP 151-168-specific T cells induce inflammation and demyelination of preferentially PNS myelin including nerves in the hind leg and spinal roots. The authors suggested two possibilities to explain their result. The first is that the distinct inflammation sites may be attributable to qualitative differences in MBP isoform composition in the CNS and PNS. The MBP 151-168 epitope, which is encoded by exons 6 and 7, exists only in the 18.5- and ...
The presence of autoreactive T-cell clones in the T-cell repertoire is determined in the thymus, but activation and expansion of naïve clonal populations is regulated in the periphery by antigen encounter, homeostatic mechanisms, and the inhibitory network of Tregs. Here, we demonstrate that naïve CD4+ T cells responsive to the β-cell autoantigens GAD65 and proinsulin are present in the T-cell repertoire at birth at a frequency that appears to be associated with HLA class II genotype. Neonatal naïve T cells were highly sensitive to IL-7, but antigen presentation was inefficient at birth and rescued by 8 months of age. We predict that these factors collectively influence the likelihood of type 1 diabetes-related autoimmunity, and in part explain the initial rarity of autoimmune seroconversion prior to 6 months of age and subsequent high incidence of seroconversion seen at ∼1 year of age (4,5).. Autoreactive T cells in man are difficult to consistently and reliably demonstrate in vitro ...
Golga7 - Golgin subfamily A member 7 - Mus musculus (Mouse) - Golga7 gene & protein
May be involved in protein transport from Golgi to cell surface. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS (By similarity).
Human autoreactive T cells recognize CD1b and phospholipids. - CAMS Oxford Institute
In contrast with the common detection of T cells that recognize MHC, CD1a, CD1c, or CD1d proteins, CD1b autoreactive T cells have been difficult to isolate in humans. Here we report the development of polyvalent complexes of CD1b proteins and carbohydrate backbones (dextramers) and their use in identifying CD1b autoreactive T cells from human donors. Activation is mediated by αβ T-cell receptors (TCRs) binding to CD1b-phospholipid complexes, which is sufficient to activate autoreactive responses to CD1b-expressing cells. Using mass spectrometry and T-cell responses to scan through the major classes of phospholipids, we identified phosphatidylglycerol (PG) as the immunodominant lipid antigen. T cells did not discriminate the chemical differences that distinguish mammalian PG from bacterial PG. Whereas most models of T-cell recognition emphasize TCR discrimination of differing self and foreign structures, CD1b autoreactive T cells recognize lipids with dual self and foreign origin. PG is rare in the
GOLGA3 polyclonal antibody - (PAB6866) - Products - Abnova
Goat polyclonal antibody raised against synthetic peptide of GOLGA3. A synthetic peptide corresponding to human GOLGA3. (PAB6866) - Products - Abnova
There may be some significant variations in HEp-2 immunofluorescence assay (IFA) staining depending on the manufacturer of HEp-2 cell slides employed. Some autoantigens and epitopes may not be available in some cell preparations. To ensure that the HEp-2 slide in use can detect anti-Ro60/SS-A and anti-La/SS-B staining, appropriate reference materials, such as those from www.AutoAb.org, should be used to verify the HEp-2 slide and other reagents employed in the assay. Experts in the field also advise to have low titer positive controls to test each new batch of HEp-2 slides for appropriate sensitivity. If well-defined standards for anti-Ro60/SSA and anti-La/SS-B do not show nuclear staining, this is a clear indication that the IFA has not been optimized for those autoantigens. The Ro60 autoantigen, in particular, seems to be labile and can be extracted by mild solvents and even prolonged exposure to some buffers. Anti-La/SS-B - in general, high titer positive anti-La/SS-B sera as determined by ...
Background: A 20-year longitudinal study of over 12,000 women shows a higher epidemiologic risk for OvCa and other cancers among women with infertility (Brinton et al, 2005, Epidemiology). In recent studies of an autoimmune etiology for some women with infertility we identified ovarian autoantigens. Several of the autoantigens were also reported as autoantigens in OvCa. Therefore the objective was to determine if there are commonly occurring autoantibodies in sera of women with infertility and OvCa in order to identify a potential risk group that would benefit from closer monitoring and earlier detection of OvCa.. Methods: We assessed antibodies to eight recombinant proteins previously identified as OvCa markers or as autoantigens in infertility. Sera (1:100) from women with infertility (n=28), malignant OvCa (n=21), benign ovarian tumors (n=9) and healthy control women without cancer (n=6) were assessed against the antigens (50 ng/well) in standard immunoassays. The antigens included ...
SPA17 Gene - GeneCards | SP17 Protein | SP17 Antibody
Complete information for SPA17 gene (Protein Coding), Sperm Autoantigenic Protein 17, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
GOLGA6A Gene - GeneCards | GOG6A Protein | GOG6A Antibody
Complete information for GOLGA6A gene (Protein Coding), Golgin A6 Family Member A, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
GOLGA2/GM130 Antibody 11308-1-AP | Proteintech
GOLGA2/GM130 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC), Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, dog samples. Cat.No. 11308-1-AP.
Intracellular Redistribution of Truncated La Protein Produced...
Intracellular Redistribution of Truncated La Protein Produced by Poliovirus 3Cpro-Mediated Cleavage: The La autoantigen (also known as SS-B), a cellular RNA bin
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90. Thymic expression of autoantigens correlates with resistance to autoimmune disease. Journal of Pain and Symptom Management, 20(4), 253в258. D.
Tamoxifen induces apoptosis through cancerous inhibitor of protein phosphatase 2A-dependent phospho-Akt inactivation in...
Tamoxifen, a selective estrogen receptor (ER) modulator, may affect cancer cell survival through mechanisms other than ER antagonism. In the present study, we tested the efficacy of tamoxifen in a panel of ER-negative breast cancer cell lines and examined the drug mechanism. In total, five ER-negative breast cancer cell lines (HCC-1937, MDA-MB-231, MDA-MB-468, MDA-MB-453 and SK-BR-3) were used for in vitro studies. Cellular apoptosis was examined by flow cytometry and Western blot analysis. Signal transduction pathways in cells were assessed by Western blot analysis. The in vivo efficacy of tamoxifen was tested in xenograft nude mice. Tamoxifen induced significant apoptosis in MDA-MB-231, MDA-MB-468, MDA-MB-453 and SK-BR-3 cells, but not in HCC-1937 cells. Tamoxifen-induced apoptosis was associated with inhibition of cancerous inhibitor of protein phosphatase 2A (CIP2A) and phospho-Akt (p-Akt) in a dose-dependent manner. Ectopic expression of either CIP2A or Akt protected MDA-MB-231 cells from tamoxifen
Identification of Novel Autoantigen in Type 1 Diabetes Using Expression-Based Genome-Wide Association Approach | Global...
Home » Noncommunicable and/or Chronic Disease » Diabetes » Identification of Novel Autoantigen in Type 1 Diabetes Using Expression-Based Genome-Wide Association ...
GOLGA6L2 | definition of GOLGA6L2 by Medical dictionary
Looking for online definition of GOLGA6L2 in the Medical Dictionary? GOLGA6L2 explanation free. What is GOLGA6L2? Meaning of GOLGA6L2 medical term. What does GOLGA6L2 mean?
Autoantigens: the critical partner in initiating and propagating systemic autoimmunity.<...
TY - JOUR. T1 - Autoantigens: the critical partner in initiating and propagating systemic autoimmunity.. AU - Duan-Porter, W.D.. AU - Casciola-Rosen, L.. AU - Rosen, A.. N1 - Cited By :4 Export Date: 26 December 2018 Correspondence Address: Duan-Porter, W.D.. PY - 2005. Y1 - 2005. N2 - The increasing recognition that cancer is frequently associated with an autoantibody response, and observations that systemic autoimmunity is sometimes associated with the diagnosis of a variety of malignancies (many detected near the onset of autoimmune disease), strongly underscore a potential mechanistic connection between cancer immunity and systemic autoimmunity. Accumulating data suggest that autoantigens are critical partners in driving the autoimmune response. Furthermore, unique changes in antigen expression and conformation in the immunizing tumor and the target tissue may play a role in antigen selection and ongoing damage. This construct has important implications for diagnosis, monitoring, and ...
Klk13 is a Putative Autoantigen During the Immunopathogenesis of Experimental Autoimmune Lacrimal Keratoconjunctivis | IOVS |...
Purpose: : Preliminary studies have identified Klk13 as a putative autoantigen during the immunopathogenesis of experimental autoimmune lacrimal keratoconjunctivitis (ALKC). To further evaluate the role of Klk13 during ALKC we assessed i) the capacity of CD4+ T cells from Klk13-immunized mice to respond to ocular surface antigens present in cornea and conjunctiva of ALKC mice and ii) the ability of Klk13 to exacerbate ALKC in vivo. Methods: : A co-culture experiment was performed to determine if immunization with Klk13 potentiates the proliferative response of CD4+ T cells from ALKC mice. CD4+ T cells were isolated from the spleen and cervical lymph nodes of Klk13-immunized (10µg) female C57BL/6 mice exposed to desiccating stress (DS; subcutaneous scopolamine injections (0.5 mg/0.2 ml) TID, humidity ,40%, and continuous air flow) for 10 days and mixed with minced cornea and conjunctiva from 10 day DS or control mice. Cells were co-cultured for 4 days and T cell proliferation was measured by WST ...
DI-fusion Cloning of candidate autoantigen carboxypeptidase H from a...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Peripheral and islet interleukin-17 pathway activation characterizes human autoimmune diabetes and promotes cytokine-mediated β...
OBJECTIVE CD4 T-cells secreting interleukin (IL)-17 are implicated in several human autoimmune diseases, but their role in type 1 diabetes has not been defined. To address the relevance of such cells, we examined IL-17 secretion in response to β-cell autoantigens, IL-17A gene expression in islets, and the potential functional consequences of IL-17 release for β-cells. RESEARCH DESIGN AND METHODS Peripheral blood CD4 T-cell responses to β-cell autoantigens (proinsulin, insulinoma-associated protein, and GAD65 peptides) were measured by IL-17 enzyme-linked immunospot assay in patients with new-onset type 1 diabetes (n = 50). mRNA expression of IL-17A and IFNG pathway genes was studied by qRT-PCR using islets obtained from subjects who died 5 days and 10 years after diagnosis of disease, respectively, and from matched control subjects. IL-17 effects on the function of human islets, rat β-cells, and the rat insulinoma cell line INS-1E were examined. RESULTS A total of 27 patients (54%) showed ...
Giantin is the major Golgi autoantigen in human anti-Golgi complex sera | Arthritis Research & Therapy | Full Text
Anti-Golgi complex antibodies (AGAs) are primarily associated with systemic lupus erythematosus and Sjögrens syndrome. Here we report on the immunoreactivity of AGAs against five Golgi autoantigens (giantin, golgin-245, golgin-160, golgin-95/GM130, and golgin-97) and provide data from epitope mapping on the most common Golgi autoantigen, namely giantin. A total of 80 human sera containing AGAs, as defined by indirect immunofluorescence on HEp-2 cells, were analyzed by ELISA using recombinant autoantigens and immunoprecipitation. The proportion of AGA sera that reacted with the five Golgi autoantigens was correlated with the molecular mass of the Golgi antigens. Autoantibodies to giantin, the largest Golgi autoantigen, were the predominant AGAs, being found in 50% of the AGA sera. Epitope mapping of giantin was performed using six recombinant fragments spanning the entire protein. Antigiantin-positive sera with low titer autoantibodies recognized epitopes in the carboxyl-terminal fragments that are
B cell apotopes of the 60-kDa Ro/SSA and La/SSB autoantigens<...
TY - JOUR. T1 - B cell apotopes of the 60-kDa Ro/SSA and La/SSB autoantigens. AU - Reed, Joanne. AU - Jackson, Michael. AU - Gordon, Thomas. PY - 2008. Y1 - 2008. M3 - Article. VL - 31. SP - 263. EP - 267. JO - Journal of Autoimmunity. JF - Journal of Autoimmunity. SN - 0896-8411. IS - 3. M1 - CC. ER - ...
Human RNA Helicase A Is a Lupus Autoantigen That Is Cleaved During Apoptosis | The Journal of Immunology
In this study we describe a novel autoantigen targeted by sera from patients with SLE. This autoantigen is cleaved during apoptosis. Sequencing of a tryptic peptide, m.w., an experimentally demonstrated RNA binding property, and immunological cross-reactivity reveal that this autoantigen is identical with human RHA. This study, to our knowledge, is the first to report that RHA is cleaved during apoptosis. Our results show that RHA is cleaved into at least three fragments (F1, F2, and F3 in Figs. 4⇑ and 5⇑) within 6 h after anti-Fas activation. The largest band, F1, appears in an early stage of apoptosis, and the size of this fragment matches the size of the in vitro product after digestion of RHA with caspase-3. These results suggest that caspase-3 contributes to the cleavage of RHA in an early stage of apoptosis. It is possible that other proteases participate in the production of F2 and F3, which appear later in apoptosis. Indeed, a recent study revealed the possibility that both caspase-3 ...
JCI - Autoreactive T effector memory differentiation mirrors β cell function in type 1 diabetes
In type 1 diabetes, cytotoxic CD8+ T cells with specificity for β cell autoantigens are found in the pancreatic islets, where they are implicated in the destruction of insulin-secreting β cells. In contrast, the disease relevance of β cell-reactive CD8+ T cells that are detectable in the circulation, and their relationship to β cell function, are not known. Here, we tracked multiple, circulating β cell-reactive CD8+ T cell subsets and measured β cell function longitudinally for 2 years, starting immediately after diagnosis of type 1 diabetes. We found that change in β cell-specific effector memory CD8+ T cells expressing CD57 was positively correlated with C-peptide change in subjects below 12 years of age. Autoreactive CD57+ effector memory CD8+ T cells bore the signature of enhanced effector function (higher expression of granzyme B, killer-specific protein of 37 kDa, and CD16, and reduced expression of CD28) compared with their CD57- counterparts, and network association modeling ...
TBX22 protein (human) - STRING interaction network
Nuclear autoantigen Sp-100; Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 according to PubMed-11909962. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA according to PubMed-15247905. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through intera [...] (885 aa ...
Identification of a novel autoantigen UACA in patients with panuveitis. - PubMed - NCBI
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Your Pharmacy: Does teva 5312 500 treats stds express shipping!
And those of stds 5312 teva does 500 treats placebo, early pregnancy loss mechanisms and the kidney in the work-up for vaginal and bladder neck competence (unless it has been dened: Brg1 or brm (human homologs of many operations. For practical purposes, purely night-time wetting some medical problems which can be applied to the surrounding structures and processus vaginalis. This effect may depend on antibacterial agents antiviral drugs in that high-afnity binding of epidermal growth factor receptor actions have been greater than 200 ml, remove the drain, and immediately rinse it under water as needed, leaving the artery in the clinical picture, it is generally responsible for causing gallstones in the. Effect of this study most likely to develop after birth, 7. Trophic lesions e.G.. This is due mainly to be due to sphincter enhancement procedures. Carrington mn, salter rd, cresswell p, ting jr evidence for autoantigen presentation by class i restriction. 1997, cancer res 31:4731. Cells of ...
Research programme: islet cell autoantigen 1 therapeutics - Inhibiton Therapeutics - AdisInsight
Inhibiton Therapeutics was developing islet cell autoantigen-1 (ICA 1) compounds for the treatment of cancer. This programme was being conducted under a
Islet cell autoantigen 1 / ICA1 antibody | acris-antibodies.com
Kit Component:- KN202149G1, ICA1 gRNA vector 1 in pCas-Guide vector- KN202149G2, ICA1 gRNA vector 2 in pCas-Guide vector- KN202149D, donor vector…
Islet cell autoantigen 1 / ICA1 antibody | acris-antibodies.com
Kit Component:- KN202149G1, ICA1 gRNA vector 1 in pCas-Guide vector- KN202149G2, ICA1 gRNA vector 2 in pCas-Guide vector- KN202149D, donor vector…
Adrian Liston - With a Graph and a Map
We had a wonderful meandering conversation about genetics, history and evolution. Everybody was quite excited about canine genetics, the genome has now been sequenced, and the different breeds of dogs have been separated for about 30 generations, each has unique medical problems. SNP linkage between individuals in the strains allows rapid location of the genetic region, and then SNP linkage between strains allows gene identification, as dog haplotypes are quite small. As the conversation turned to evolution against autoimmunity, we were discussing why autoantibody targets are generally evolutionary conserved regions. Olle and myself were saying that immunogenic autoantigens would be selected against where possible, such that only regions with vital function (and thus evolutionarily conserved) would be left as autoantibody targets. This made Chris wonder how much autoimmunity could be altered by evolutionary selection, as it usually occurs after reproductive age. This was quite interesting, ...
Transgenically Induced GAD Tolerance Curtails the Development of Early β-Cell Autoreactivities but Causes the Subsequent...
Objective: To study how tolerance to GAD65 affects the development of autoimmunity to other β-cell autoantigens (β-CAAs) in GAD65-transgenic (GAD-tg) NOD mice. Research Designs and Methods: We used ELISPOT to characterize the frequency and functional phenotype of T-cell responses to GAD65 and other β-CAAs at different ages in GAD-tg mice and their NOD mouse littermates. Results: In young GAD-tg mice, Th1 responses to GAD65s dominant determinants were 13−18% of those in young NOD mice. This coincided with a great reduction in Th1 responses to other β-CAAs. Evidently, GAD65-reactive T-cells are important for activating and/or expanding early autoreactivities in NOD mice. As GAD-tg mice aged, their T-cell responses to GAD65 remained low, but they developed supernormal splenic and pancreatic lymph node T-cell autoimmunity to other β-CAAs. Apparently, the elimination/impairment of many GAD65-reactive T-cells allowed other β-CAA-reactive T-cells to eventually expand to a greater extent, ...
Papers in the shared collection Interesantes | Read by QxMD
PURPOSE OF REVIEW: Systemic lupus erythematosus (SLE) is characterized by autoantibodies directed against nuclear autoantigens normally concealed from immune recognition in healthy individuals. Here, we summarize recently identified mechanisms of abnormal cell death leading to exposure and aberrant processing of nucleoprotein self antigens, and discuss their role in the SLE pathogenesis. RECENT FINDINGS: During the past few years, the unveiling of several new forms of cell death has expanded our understanding beyond the simple view of apoptotic versus necrotic cell death ...
LA/SS-B Protein Human Recombinant | SSB Antigen | ProSpec
LA/SS-B Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 47.6 kDa.
Identification of Tumor-associated Autoantigens for the Diagnosis of Colorectal Cancer in Serum Using High Density Protein...
Blood is the optimal source for the diagnostic screening of large human populations for non-invasive markers. Serum and plasma are easily obtained, and moreover blood circulation facilitates the contact with every body tissue, including representative tumor antigens. However, tumor leakage antigens are probably present at the very low range of concentration in plasma, and they probably suffer from extensive proteolysis in a relatively short period (44). Therefore, the search for tumor-specific antigens in blood is a complicated task. Approaches based on a peptide search (peptidomics), such as SELDI, are prone to artifacts due to variability issues and require ultraextensive standardization for every step of the procedure, rendering them unsuitable for routine clinical use.. Antibodies are very stable serum molecules with a long tradition of use in immunoassays, which facilitates their standardization. Autoantibodies present in the serum of patients appear to be a promising alternative for ...
Evidence presented in this report shows that introduction of a novel autoantigen into the spontaneous disease process in the NOD mouse had no significant impact on incidence of type 1 diabetes and only led to a minimal acceleration of the disease course. This was quite unexpected, because antigenic spreading is responsible for relapses in the EAE model for multiple sclerosis and was therefore thought of as a hallmark event in the pathogenesis of T-cell-mediated autoimmune disorders. One could hypothesize, based on these findings, that the autoimmune process in the NOD model is already driven by a variety of antigens at the time when nucleoprotein-specific T-cells are being activated in the PDLNs. Therefore, the impact of one additional antigen is rather limited. Conversely, attempting therapeutic elimination of antigenic specificities that behave like the response to the nucleoprotein neo-antigen will probably have only a small impact in reducing disease incidence and severity, if this ...
Modulating co-stimulation | SpringerLink
The modulation of co-stimulatory pathways represents a novel therapeutic strategy to regulate autoimmune diseases. Auto-reactive CD4+ T cells play a critical role in initiating the immune response leading to inflammation and autoimmune diseases. Blocking co-stimulatory signals prevents T-cell activation, thus diminishing autoimmune responses and possibly preventing the progression of autoimmune disease. Blockade of several co-stimulatory pathways has been investigated in animal models and has led to clinical trials testing specific blocking agents in humans. In this review we will describe the role of co-stimulatory pathways, primarily the CD28-B7 pathway, in autoimmune diseases, and we will present in vivo and in vitro studies supporting the efficacy of co-stimulation blockade in animal models of autoimmune disease. Finally, we will discuss the clinical therapeutic efficacy of blocking monoclonal antibodies in preventing or reducing auto-antigen driven T-cell activation in humans with ...
KAKEN - Research Projects | SEREX ANALYSIS OF IPF PATIENS : DEMONSTRATION OF SOME AUTOANTIGENS REACTED WITH CD4 POSITIVE T...
Principal Investigator：KUROSU Katsushi, Project Period (FY)：2003 - 2004, Research Category：Grant-in-Aid for Scientific Research (C), Section：一般, Research Field：Respiratory organ internal medicine
The lollipop plot above illustrates recurrent (observed in 3 or more out of 4440 TCGA tumor samples from 15 cancer types) and therefore potentially oncogenic missense mutations (click on Show Cancer Mutations). The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. ...
golga2 Expression [Xenopus] - Xenbase Gene Catalog
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari. ...
golga7ba - PrimePCR Assay and Template | Life Science | Bio-Rad
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
MKB-10 Poglavje XVII: Prirojene malformacije, deformacije in kromosomske nenormalnosti - Wikipedija, prosta enciklopedija
Mednarodno klasifikacijo bolezni in sorodnih zdravstvenih problemov 10-ta revizija (MKB-10) objavlja Svetovna zdravstvena organizacija (WHO).. Ta stran vsebuje MKB-10 Poglavje XVII: Prirojene malformacije, deformacije in kromosomske nenormalnosti. ...
г. Сызрань - EPYS
Сызрань - располагается на правом берегу реки Волги в 130 километрах от Самары вниз по течению, у впадения в Волгу Сызранки, Крымзы и Кубры. Название город получил по имени реки и в переводе с тюркского означает «овраг», «низина», «болотистая местность». Город основан в 1683 году по указу царей Петра и Иоанна Алексеевичей как военная крепость воеводой Григорием Козловским. Закладка города в XVII веке была вызвана необходимостью обеспечивать безопасность торгового пути между северными и южными областями государства Российского. В 1781 году Сызрань ...
Riemekasten G, Hahn BH (August 2005). "Key autoantigens in SLE". Rheumatology. 44 (8): 975-82. doi:10.1093/rheumatology/keh688 ...
Eric John Holborow
cite journal}}: Cite journal requires ,journal= (help) Toh BH (1979). "Smooth muscle autoantibodies and autoantigens". Clin Exp ...
Group 1 CD1-restricted T cells
Shamshiev A, Donda A, Carena I, Mori L, Kappos L, De Libero G (May 1999). "Self glycolipids as T-cell autoantigens". European ...
Le Romancer M, Reyl-Desmars F, Cherifi Y, Pigeon C, Bottari S, Meyer O, Lewin MJ (1994). "The 86-kDa subunit of autoantigen Ku ... Ku80 has been referred to by several names including: Lupus Ku autoantigen protein p80 ATP-dependent DNA helicase 2 subunit 2 X ... Cao QP, Pitt S, Leszyk J, Baril EF (1994). "DNA-dependent ATPase from HeLa cells is related to human Ku autoantigen". ... Myung K, He DM, Lee SE, Hendrickson EA (1997). "KARP-1: a novel leucine zipper protein expressed from the Ku86 autoantigen ...
Teuscher C, Wild GC, Tung KS (1982). "Immunochemical analysis of guinea pig sperm autoantigens". Biol. Reprod. 26 (2): 218-229 ...
... can generate autoantigens by cleaving in disordered regions and linker regions of antigens exposing new epitopes and ... Darrah E, Rosen A (April 2010). "Granzyme B cleavage of autoantigens in autoimmunity". Cell Death and Differentiation. 17 (4): ...
Individual autoantigens are deposited in an array of dots onto a surface such as polystyrene. A single array could consist of ... Autoantigens present on the blebs of apoptotic cells are also prone to modification, which can increase their immunogenicity. ... Similar to the flow cytometry method of ANA detection, the MIA uses wells containing autoantigens and HEp-2 extract coated ... Blebs on apoptotic cells contain nearly all the autoantigens found in SLE, and phagocytes bind these apoptotic cells and ...
Teuscher C, Wild GC, Tung KS (1982). "Immunochemical analysis of guinea pig sperm autoantigens". Biology of Reproduction. 26 (2 ...
RoSSA interacts with autoantigens in patients with Sjögren's syndrome and systemic lupus erythematosus. In addition, the ... Frank MB, McCubbin VR, Heldermon C (January 1995). "Expression and DNA binding of the human 52 kDa Ro/SSA autoantigen". The ... The 52- and 60-kD Ro/SSA autoantigens are encoded by separate genes". The Journal of Clinical Investigation. 87 (1): 177-86. ... Keech CL, Gordon TP, McCluskey J (May 1996). "Structural differences between the human and mouse 52-kD Ro autoantigens ...
Dohlam, JG; Lupas, A; Carson, M (1993). "Long charge-rich alpha-helices in systemic autoantigens". Biochem Biophys Res Commun. ... A physicochemical analysis of common structural motifs present in 109 human autoantigens revealed that tropomyosins have the ... highest number of such motifs, and thus a very high propensity to act as autoantigens. In addition to the role tropomyosins ...
Anti-Hu associated encephalitis
Blaes F, Fühlhuber V, Preissner KT (2007). "Identification of autoantigens in pediatric opsoclonus-myoclonus syndrome". Expert ...
1993). "Islet cell autoantigen 69 kD (ICA69). Molecular cloning and characterization of a novel diabetes-associated autoantigen ... "Entrez Gene: ICA1 islet cell autoantigen 1, 69kDa". Nepom GT (1996). "Glutamic acid decarboxylase and other autoantigens in ... Islet cell autoantigen 1 is a protein that in humans is encoded by the ICA1 gene. This gene encodes a protein with an arfaptin ... 1998). "Anti-BSA antibodies do not cross-react with the 69-kDa islet cell autoantigen ICA69". J. Autoimmun. 11 (3): 223-31. doi ...
Fritsch et al.: Characterization of autoreactive T cells to the autoantigens RA33 (hnRNP A2) and filaggrin in patients with ... Steiner et al.: Purification and partial sequencing of the nuclear autoantigen RA33 shows that it is indistinguishable from the ... RA33, also known as heterogeneous nuclear ribonucleoprotein A2/B1, is an autoantigen in human systemic autoimmune diseases. In ...
Solute carrier family 25 (mitochondrial carrier; Graves disease autoantigen), member 16 is a protein in humans that is encoded ... "Entrez Gene: Solute carrier family 25 (mitochondrial carrier; Graves disease autoantigen), member 16". Retrieved 2012-11-27. v ...
Kelch-like protein 12
September 2005). "Identification of specific autoantigens in Sjögren's syndrome by SEREX". Immunology. 116 (1): 53-63. doi: ...
It causes thyrocyte damage and the release of autoantigens. Iodine also promotes follicular cell apoptosis and has an influence ... They can present thyroid autoantigens and initiate autoimmune thyroid disease. Susceptibility alleles are not consistent in ...
... and ILF3 have been identified as autoantigens in mice with induced lupus, in canine systemic rheumatic autoimmune disease ... "ILF2 and ILF3 are autoantigens in canine systemic autoimmune disease". Scientific Reports. 8 (1): 4852. Bibcode:2018NatSR... ...
ILF2 and ILF3 have been identified as autoantigens in mice with induced lupus, in canine systemic rheumatic autoimmune disease ... "ILF2 and ILF3 are autoantigens in canine systemic autoimmune disease". Scientific Reports. 8 (1): 4852. Bibcode:2018NatSR... ...
The autoantigen is the nucleoporin 62kDA protein. Wesierska-Gadek J, Hohenuer H, Hitchman E, Penner E (1996). "Autoantibodies ...
"Muscle autoantigens in thyroid associated ophthalmopathy: the limits of molecular genetics". J. Endocrinol. Invest. 16 (7): 533 ...
Elisei R, Weightman D, Kendall-Taylor P, Vassart G, Ludgate M (1993). "Muscle autoantigens in thyroid associated ophthalmopathy ...
2011). "Autoantigen discovery with a synthetic human peptidome". Nature Biotechnology. 29 (6): 535-541. doi:10.1038/nbt.1856. ...
Calcium-binding atopy-related autoantigen 1 is a protein that in humans is encoded by the CBARA1 gene. Mutations in this gene ... "Entrez Gene: CBARA1 calcium binding atopy-related autoantigen 1". Mojbafan M, Nojehdeh ST, Rahiminejad F, Nilipour Y, ... "Isolation of cDNA clones coding for IgE autoantigens with serum IgE from atopic dermatitis patients". FASEB J. 12 (14): 1559-69 ...
Neutrophil cytosolic factor 4
Grandvaux N, Grizot S, Vignais PV, Dagher MC (Feb 1999). "The Ku70 autoantigen interacts with p40phox in B lymphocytes". J. ... Grandvaux N, Grizot S, Vignais PV, Dagher MC (1999). "The Ku70 autoantigen interacts with p40phox in B lymphocytes". J. Cell ...
February 2011). "Investigations of caspr2, an autoantigen of encephalitis and neuromyotonia". Annals of Neurology. 69 (2): 303- ...
The chaperone protein acts as auto antigen in atherosclerosis. Increased oxidative stress causes the formation of high-density ...
Lu L, Tai G, Hong W (October 2004). "Autoantigen Golgin-97, an effector of Arl1 GTPase, participates in traffic from the ... Lu L, Hong W (September 2003). "Interaction of Arl1-GTP with GRIP domains recruits autoantigens Golgin-97 and Golgin-245/p230 ... "Entrez Gene: GOLGA1 golgi autoantigen, golgin subfamily a, 1". Lu L, Hong W (September 2003). "Interaction of Arl1-GTP with ... "Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjögren's syndrome". Arthritis and Rheumatism. 40 ...
"Entrez Gene: GOLGA5 golgi autoantigen, golgin subfamily a, 5". Satoh, Ayano; Wang Yanzhuang; Malsam Jörg; Beard Matthew B; ...
Fritzler MJ, Hamel JC, Ochs RL, Chan EK (Jul 1993). "Molecular characterization of two human autoantigens: unique cDNAs ... "Entrez Gene: GOLGA3 golgi autoantigen, golgin subfamily a, 3". Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and ...
Winqvist O, Karlsson FA, Kämpe O (June 1992). "21-Hydroxylase, a major autoantigen in idiopathic Addison's disease". The Lancet ...
Nucleosomes are major T and B cell autoantigens in systemic lupus erythematosus
We present evidence that nucleosomes are major autoantigens in human SLE and that antinucleosomal antibodies are highly ... Nucleosomes are major T and B cell autoantigens in systemic lupus erythematosus Arthritis Rheum. 2000 Oct;43(10):2307-15. doi: ... T cell responses to autoantigens, including DNA, have been reported only incidentally in SLE patients. Nevertheless, in murine ... Conclusion: We present evidence that nucleosomes are major autoantigens in human SLE and that antinucleosomal antibodies are ...
CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate: Cell
The lupus autoantigens and the pathenogenesis of systemic lupus erythromatosus.. Arthritis Rheum. 1986; 29: 457-460. *Scopus ( ... CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate. *. Hisato Saitoh. Hisato Saitoh ... Analysis of anti-centromere autoantibodies using cloned autoantigen CENP-B.. in: Proc. Natl. Acad. Sci. (USA).84. 1987: 4979- ... CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate ...
Charakterisierung des Autoantigens "mitochondriale Malat-Dehydrogenase" bei der equinen rezidivierenden Uveitis
... that mMDH can be defined as an autoantigen. Whiteskby´s postulates were taken as criteria for the definition of an autoantigen ... that mMDH can be defined as an autoantigen. Whiteskby´s postulates were taken as criteria for the definition of an autoantigen ... dass mMDH als Autoantigen definiert werden kann. Als Definitionskriterien für ein Autoantigen dienten die Witebskyschen ... dass mMDH als Autoantigen definiert werden kann. Als Definitionskriterien für ein Autoantigen dienten die Witebskyschen ...
T Cell Responses to Neural Autoantigens Are Similar in Alzheimer's Disease Patients and Age-Matched Healthy Controls
International Workshop on Lessons from Animal Models for Human Type 1 Diabetes: analyzing target autoantigens of humoral...
International Workshop on Lessons from Animal Models for Human Type 1 Diabetes: analyzing target autoantigens of humoral ... International Workshop on Lessons from Animal Models for Human Type 1 Diabetes: analyzing target autoantigens of humoral ... analyzing target autoantigens of humoral immunity in nonobese diabetic mice. Ann N Y Acad Sci. 2002 Apr; 958:1-2. ...
Abnormal Cytokine Response and Autoantigen Production in IgA-Producing Subpopulations in IgA Nephropathy
Browsing by Subject "Autoantigens"
Cathepsin S dominates autoantigen processing in human thymic dendritic cells
IMSEAR at SEARO: Autoantigen Ku and its role in multiple cellular processes.
Ku-autoantigen acts as a potential transcription factor for several RNA polymerase II genes and RNA polymerase I gene. Ku is ... Therefore, Ku-autoantigen is a very important cellular factor which plays important role in the multiple cellular processes. ... Autoantigen Ku and its role in multiple cellular processes. Indian Journal of Experimental Biology. 1997 Dec; 35(12): 1261-72. ... Ku is a DNA binding protein composed of 70 and 80 kDa subunits which was discovered as autoantigen in a patient with ...
Increased frequencies of Sm and nRNP autoantibodies in American blacks compared to whites with systemic lupus erythematosus
Research Labs and Institutes | Johns Hopkins Medicine
Research Areas: autoantigens, autoimmune diseases, cytotoxic granule proteases, peptidylarginine deiminase enzymes, rheumatoid ... We focus on the fate of autoantigens in target cells during various circumstances, such as viral infection, relevant immune ... We currently focus on two principal areas: (1) defining the mechanisms that generate citrullinated autoantigens in vivo in ... Our current research involves identifying the antigen targets of autoimmune diseases, investigating the autoantigens targeted ...
FLNB gene: MedlinePlus Genetics
RNA components of each autoantigen are shown with arrowheads. Total RNA and the pattern by anti-Sm serum are shown as ... Analysis of RNA components of autoantigens. K562 cell extract from 107 cells was immunoprecipitated by prototype sera for anti- ... Analysis of protein components of autoantigens (8% SDS-PAGE). 35S-methionine labeled K562 cell extract was immunoprecipitated ... Analysis of protein components of autoantigens (12.5% SDS-PAGE). 35S-methionine labeled K562 cell extract was ...
Heart-specific autoantibodies induced by Coxsackievirus B<sub>3</sub>: Identification of heart...
These sera were used to indentify the target myocardial autoantigen(s). Sera pools were made during the peak of the early, ... These sera were used to indentify the target myocardial autoantigen(s). Sera pools were made during the peak of the early, ... These sera were used to indentify the target myocardial autoantigen(s). Sera pools were made during the peak of the early, ... These sera were used to indentify the target myocardial autoantigen(s). Sera pools were made during the peak of the early, ...
Relapsing Polychondritis Workup: Laboratory Studies, Imaging Studies, Other Tests
Characterization and epitope mapping of human monoclonal antibodies to PDC-E2, the immunodominant autoantigen of primary...
N2 - Further to define the epitopes of PDC-E2, the major autoantigen in primary biliary cirrhosis (PBC), we have developed and ... AB - Further to define the epitopes of PDC-E2, the major autoantigen in primary biliary cirrhosis (PBC), we have developed and ... Further to define the epitopes of PDC-E2, the major autoantigen in primary biliary cirrhosis (PBC), we have developed and ... All five monoclonals, but none of the other mitochondrial autoantigens were specific for PDC-E2. In fact, although affinity ...
Emerging Infectious Determinants of Chronic Diseases - Volume 12, Number 7-July 2006 - Emerging Infectious Diseases journal -...
Guttate Psoriasis: Overview of Guttate Psoriasis, Pathophysiology of Guttate Psoriasis, Epidemiology of Guttate Psoriasis
Revisiting tolerance induced by autoantigen in incomplete Freund's adjuvant<...
title = "Revisiting tolerance induced by autoantigen in incomplete Freunds adjuvant",. abstract = "Injection of autoantigens ... N2 - Injection of autoantigens in IFA has been one of the most effective ways of preventing experimental, T cell-mediated, ... AB - Injection of autoantigens in IFA has been one of the most effective ways of preventing experimental, T cell-mediated, ... Injection of autoantigens in IFA has been one of the most effective ways of preventing experimental, T cell-mediated, ...
Autoantibodies and autoantigens in autoimmune hepatitis: important tools in clinical practice and to study pathogenesis of the...
Characterization of the autoantigen-autoantibody repertoire continues to be an attractive and important tool to get access to ... Cytochrome P450 1A2 has been identified as the target-autoantigen of anti-LM autoantibodies in both APECED-related AIH and ... Family 1 of UDP-glycuronosyltransferases has been identified as the target-autoantigen of anti-LKM-3. For these reasons the ... The current classification of AIH and the several autoantibodies/target-autoantigens found in this disease are reported. ...
SMOKING AFFECTS IMMUNE RESPONSE TO CITRULLINATED AUTOANTIGENS BEFORE CLINICAL ONSET OF RHEUMATOID ARTHRITIS IN A SOUTHERN...
HLA-A*0201-restricted T cells from humanized NOD mice recognize autoantigens of potential clinical relevance to type 1 diabetes...
HLA-A*0201-restricted T cells from humanized NOD mice recognize autoantigens of potential clinical relevance to type 1 diabetes ... HLA-A*0201-restricted T cells from humanized NOD mice recognize autoantigens of potential clinical relevance to type 1 diabetes ... Dive into the research topics of HLA-A*0201-restricted T cells from humanized NOD mice recognize autoantigens of potential ... HLA-A*0201-restricted T cells from humanized NOD mice recognize autoantigens of potential clinical relevance to type 1 diabetes ...
Furthermore, UCB Treg cells will also be shown to have significantly more clones with TCRs particular for autoantigens (28) -...
Furthermore, UCB Treg cells will also be shown to have significantly more clones with TCRs particular for autoantigens (28). * ... Furthermore, UCB Treg cells will also be shown to have significantly more clones with TCRs particular for autoantigens (28). ... UCB Treg cells may also be shown to have significantly more clones with TCRs particular for autoantigens (28). Terminal ...
The exposure of autoantigens by microparticles underlies the formation of potent inflammatory components: The microparticle...
Rather, mpICs display autoantigens vimentin and fibrinogen, and recognition of these targets by anti-citrullinated peptide ... The exposure of autoantigens by microparticles underlies the formation of potent inflammatory components: The microparticle- ... Taken together, our data suggest a unique role for platelet MPs as autoantigen-expressing elements capable of perpetuating ... The exposure of autoantigens by microparticles underlies the formation of potent inflammatory components: The microparticle- ...
Recommendations for the Use of Lyme Disease Vaccine Recommendations of the Advisory Committee on Immunization Practices (ACIP)
Aberrant Accumulation of the Diabetes Autoantigen GAD65 in Golgi Membranes in Conditions of ER Stress and Autoimmunity. |...
Frontiers | T Cell-Mediated Beta Cell Destruction: Autoimmunity and Alloimmunity in the Context of Type 1 Diabetes
The cation efflux transporter ZnT8 (Slc30A8) is a major autoantigen in human type 1 diabetes. Proc Natl Acad Sci U S A (2007) ... Insulin as an autoantigen in NOD/human diabetes. Curr Opin Immunol (2008) 20(1):111-8. doi:10.1016/j.coi.2007.11.005 ... Identification of an MHC class I-restricted autoantigen in type 1 diabetes by screening an organ-specific cDNA library. Nat Med ... Table 1 summarizes known autoantigens in T1D development in human subjects and NOD mice and if they are recognized by CD4 or ...
Relapsing Polychondritis Workup: Laboratory Studies, Imaging Studies, Other Tests
- Moreover, several laboratories have suggested that autoantibodies against the 2-oxo acids dehydrogenase autoantigens are cross-reactive. (elsevier.com)
- A new study published on the preprint server medRxiv * assessed the presence of autoantibodies against a panel of autoantigens associated with known autoimmune diseases in both adults and children with COVID-19. (news-medical.net)
- The analysis of adult patients with COVID-19 found high levels of autoantibodies against several autoantigens such as the lung protein KCNRG, gastric ATPase, and systemic lupus erythematosus (SLE) antigen Sm-D3. (news-medical.net)
- The results further indicated that children with COVID-19 reported little or no autoantibodies against KCNRG, Sm-D3, and the other autoantigens that were tested. (news-medical.net)
- They have significant implications for proposed mechanisms on the generation of complex patterns of autoantibodies to a diverse group of autoantigens in SLE patients. (wikigenes.org)
- Various serological tests detected no autoantibodies or autoantigens. (medicaljournals.se)
- Diese besagen, dass erstens das Antigen bekannt sein muss, zweitens sich eine humorale und / oder zelluläre Immunreaktion gegen das Antigen nachweisen lassen muss, und drittens eine entsprechende Krankheit in einem Tiermodell durch Immunisierung mit dem Antigen zu induzieren ist, um das Antigen als Autoantigen definieren zu können. (uni-muenchen.de)
- Our current research involves identifying the antigen targets of autoimmune diseases, investigating the autoantigens targeted in cancers associated with rheumatic diseases and finding unique clinical biomarkers, such as the anti-HMGCR antibody specificity. (hopkinsmedicine.org)
- Antigen (or autoantigen) engages a B cell receptor directly and also is endocytosed by an antigen presenting cell (typically a dendritic cell, but B cells also serve), in which intracellular degradation generates antigenic peptides. (bmj.com)
Panel of autoantigens1
- Skin cell cultures expressed the AIRE, Foxn1, and Hoxa3 transcription factors and a panel of autoantigens. (jci.org)
- Aberrant Accumulation of the Diabetes Autoantigen GAD65 in Golgi Membranes in Conditions of ER Stress and Autoimmunity. (uchicago.edu)
- Primary Human and Rat β-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity. (ucsf.edu)
- Characterisation of the autoantigen "mitochondrial malate dehydrogenase" in the equine recurrent Uveitis Equine Recurrent Uveitis (ERU) is a wide spread disease of the eye, which is the main cause for blindness in horses worldwide. (uni-muenchen.de)
- Previous studies indicated that the enzyme mitochondrial malate dehydrogenase (mMDH) is involved in ERU-pathophysiology as an autoantigen. (uni-muenchen.de)
- All five monoclonals, but none of the other mitochondrial autoantigens were specific for PDC-E2. (elsevier.com)
- c autoimmune diseases, particularly as they relate to the role of cytotoxic granule proteases in autoimmunity and viral clearance, mechanisms of autoantigen citrullination and pathways that control immune effector functions in autoimmune diseases. (hopkinsmedicine.org)
- As models of autoimmunity against sequestered and readily accessible autoantigens, we studied experimental allergic encephalomyelitis, induced by myelin oligodendrocyte glycoprotein, proteolipid protein, myelin basic protein, and renal tubular Ag-induced interstitial nephritis. (mssm.edu)
- The impact of airborne endotoxin exposure on rheumatoid arthritis-related joint damage, autoantigen expression, autoimmunity, and lung disease. (cdc.gov)
- We have isolated and characterized a set of overlapping cDNA clones that encode the human centromere autoantigen centromere protein C(CENP-C). The identity of these clones has been established using several criteria. (cell.com)
- Ku is a DNA binding protein composed of 70 and 80 kDa subunits which was discovered as autoantigen in a patient with scleroderma-polymyositis overlap syndrome. (who.int)
- Islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP) is one of several important T1D autoantigens in standard NOD mice. (elsevier.com)
- 14-3-3 zeta is an autoantigen (a protein recognized by our own immune systems) that is thought to play a role in inflammatory arthritis, and the researchers set out with the hypothesis that it may trigger the condition. (iflscience.com)
- Charakterisierung des Autoantigens „mitochondriale Malat-Dehydrogenase" bei der equinen rezidivierenden Uveitis Die equine rezidivierende Uveitis (ERU) ist eine weit verbreitete Augenerkrankung, die weltweit die Hauptursache für das Erblinden von Pferden darstellt. (uni-muenchen.de)
- Commensal microbiota and myelin autoantigen cooperate to trigger autoimmune demyelination. (mpg.de)
- Vorausgehende Untersuchungen wiesen darauf hin, dass das Enzym mitochondriale Malat-Dehydrogenase (mMDH) als Autoantigen an der ERU beteiligt ist. (uni-muenchen.de)
- More patients showed reactivity against mMDH than to any other of the tested retinal autoantigens. (uni-muenchen.de)
- A long-term study including two horses showed that T-cell reactivity against a single retinal autoantigen can only be observed during a limited period of time. (uni-muenchen.de)
- Anti-myosin was still the most notable reactivity, even though other autoantigens were detected. (elsevier.com)
- T cell responses to autoantigens, including DNA, have been reported only incidentally in SLE patients. (nih.gov)
- We currently focus on two principal areas: (1) defining the mechanisms that generate citrullinated autoantigens in vivo in rheumatoid arthritis and (2) understanding the pathways that control the activity of the peptidylarginine deiminase (PAD) enzymes in human neutrophils. (hopkinsmedicine.org)
- We focus on the fate of autoantigens in target cells during various circumstances, such as viral infection, relevant immune effector pathways and exposure to ultraviolet radiation. (hopkinsmedicine.org)
- This group of disorders is characterized by immune reaction against thyroid autoantigens. (thyroidorg.info)
- Collectively, these results indicate the utility of humanized HLA-A*0201-expressing NOD mice in the identification of T cells and autoantigens of potential relevance to human T1D. (elsevier.com)
- We present evidence that nucleosomes are major autoantigens in human SLE and that antinucleosomal antibodies are highly specific for the disease. (nih.gov)
- International Workshop on Lessons from Animal Models for Human Type 1 Diabetes: analyzing target autoantigens of humoral immunity in nonobese diabetic mice. (umassmed.edu)
- Bonifacio E, Atkinson M, Eisenbarth G, Serreze D, Kay TW, Lee-Chan E, Singh B. International Workshop on Lessons from Animal Models for Human Type 1 Diabetes: analyzing target autoantigens of humoral immunity in nonobese diabetic mice. (umassmed.edu)
- Further to define the epitopes of PDC-E2, the major autoantigen in primary biliary cirrhosis (PBC), we have developed and characterized five human monoclonal antibodies. (elsevier.com)
- Both active and passive tolerance mechanisms, therefore, contribute to autoantigen:IFA-induced protection from autoimmune disease. (mssm.edu)
- Injection of autoantigens in IFA has been one of the most effective ways of preventing experimental, T cell-mediated, autoimmune disease in mice. (mssm.edu)
- 2016. Microneedle delivery of autoantigen for immunotherapy in type 1 diabetes . (cardiff.ac.uk)
- Ku-autoantigen acts as a potential transcription factor for several RNA polymerase II genes and RNA polymerase I gene. (who.int)
- The purpose of this thesis was to investigate the role of mMDH in ERU and to proof the hypothesis, that mMDH can be defined as an autoantigen. (uni-muenchen.de)
- The three Witebsky Postulates were all met, so the hypothesis that mMDH is an autoantigen which plays a role in the pathogenesis of ERU could be confirmed. (uni-muenchen.de)
- IMSEAR at SEARO: Autoantigen Ku and its role in multiple cellular processes. (who.int)
- Taken together, our data suggest a unique role for platelet MPs as autoantigen-expressing elements capable of perpetuating formation of inflammatory ICs. (novartis.com)
- These sera were used to indentify the target myocardial autoantigen(s). (elsevier.com)
- These studies suggest that myosin is one of the major autoantigens in Coxsackievirus B 3 -induced autoimmune myocarditis. (elsevier.com)
- Rather, mpICs display autoantigens vimentin and fibrinogen, and recognition of these targets by anti-citrullinated peptide antibodies contributes to the production of mpICs. (novartis.com)
- As mMDH was previously identified by proteomic studies as a potential autoantigen, the first of the postulates was already met prior to this investigation. (uni-muenchen.de)
- Hyper- N -glycosylated SAMD14 and neurabin-I as driver autoantigens of primary central nervous system lymphoma. (nih.gov)