Very toxic and complex pyrone derivatives from the fungus Calcarisporium arbuscula. They bind to and inhibit mitochondrial ATPase, thereby uncoupling oxidative phosphorylation. They are used as biochemical tools.

Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. (1/40)

The release of cytochrome c from the intermembrane space of mitochondria into the cytosol is one of the critical events in apoptotic cell death. In the present study, it is shown that release of cytochrome c and apoptosis induced by tumor necrosis factor alpha (TNF) in HeLa cells can be inhibited by (i) overexpression of an oncoprotein Bcl-2, (ii) Cyclosporin A, an inhibitor of the mitochondrial permeability transition pore (PTP) or (iii) oligomycin, an inhibitor of H+- ATP-synthase. Staurosporine-induced apoptosis is sensitive to Bcl-2 but insensitive to Cyclosporin A and oligomycin. The effect of oligomycin is not due to changes in mitochondrial membrane potential or to inhibition of ATP synthesis/hydrolysis since (a) uncouplers (CCCP, DNP) which discharge the membrane potential fail to abolish the protective action of oligomycin and (b) aurovertin B (another inhibitor of H+-ATP-synthase, affecting its F1 component) do not affect apoptosis. A role of oligomycin-sensitive F0 component of H+-ATP-synthase in the TNF-induced PTP opening and apoptosis is suggested.  (+info)

Influence of aurovertin on mitochondrial ATPase activity. (2/40)

Investigations have been made of the kinetic effects of the antibiotic aurovertin on the ATPase and ITPase activity of isolated rat liver mitochondrial ATPase. Unusual patterns of inhibition, decreasing slope, and increasing y-intercept values of double reciprocal plots, were observed with Mg-ATP as the substrate under various conditions. Under specified conditions, aurovertin stimulated hydrolysis of MgATP. The inhibition of MgITP hydrolysis was uncompetitive. Aurovertin eliminated the HCO3-minus stimulation of MgATP hydrolysis. The implications of these findings for the mechanism of mitochondrial ATPase are briefly discussed.  (+info)

A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles. (3/40)

An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform. Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-released enzyme is similar to other ATPase preparations from bovine heart mitochondria.  (+info)

Nucleotide sequence, organization and characterization of the atp genes and the encoded subunits of Mycoplasma gallisepticum ATPase. (4/40)

The nucleotide sequence of a 7.8 kbp DNA fragment from the genome of Mycoplasma gallisepticum has been determined. The fragment contains a cluster of nine tightly linked genes coding for the subunits of the M. gallisepticum ATPase. The gene order is I (I-subunit), B (a-subunit), E (c-subunit), F (b-subunit), H (delta-subunit), A (alpha-subunit), G (gamma-subunit), D (beta-subunit) and C (epsilon-subunit). Two open reading frames were identified in the flanking regions; one (ORFU), preceding the I gene, encodes at least 110 amino acids and the other (ORFS), following the C gene, encodes at least 90 amino acids. The deduced amino acid sequences of the various subunits are presented and discussed with regard to the structure, function and differing sensitivity of the M. gallisepticum enzyme to dicyclohexylcarbodiimide and aurovertin. The alpha- and beta-subunits of the F1 portion are well conserved (51% and 65% identity with those of Escherichia coli), whereas the gamma-, delta- and epsilon-subunits, as well as the F0-subunits, show a low percentage identity. Nonetheless, the secondary structure of the F0-subunits show a high degree of similarity to the corresponding subunits of E. coli. Two very strong potential amphipathic alpha-helices are predicted in the delta-subunit and the N-terminus of the b-subunit contains two hydrophobic helical stretches. The possible roles of these structural properties in the close association of the F1 and F0 multisubunit complexes among mycoplasmas are discussed.  (+info)

Aurovertin binds to the beta subunit of yeast mitochondrial ATPase. (5/40)

Isolated beta subunit of ATPase (F1) from yeast mitochondria does not catalyze an ATPase reaction but still binds the specific F1 inhibitor aurovertin. Binding was measured by enhancement of aurovertin fluorescence; it was as tight as that to F1-ATPase. No binding was observed with F1 or with isolated beta subunit from a single-gene nuclear yeast mutant whose F1-ATPase was resistant to aurovertin.  (+info)

Citreoviridin, a specific inhibitor of the mitochondiral adenosine triphosphatase. (6/40)

1. Citreoviridin was a potent inhibitor of the soluble mitochondrial ATPase (adenosine triphosphatase) similar to the closely related aurovertins B and D. 2. Citreoviridin inhibited the following mitochondrial energy-linked reactions also: ADP-stimulated respiration in whole mitochondria from ox heart and rat liver; ATP-driven reduction of NAD+ by succinate; ATP-driven NAD transhydrogenase and ATPase from ox heart submitochondrial particles. 3. The dissociation constant (KD) calculated by a simple law-of-mass-action treatment for the citreoviridin--ATPase complex was 0.5--4.2micron for ox-heart mitochondrial preparations and 0.15micron for rat liver mitochondria. 4. Monoacetylation of citreoviridin decreased its inhibitory potency (KD=2--25micron, ox heart; KD=0.7micron, rat liver). Diacetylation greatly decreased the inhibitory potency (KD=60--215micron, ox heart). 5. Hydrogenation of citreoviridin monoacetate diminished its inhibitory potency considerably. 6. No significant enhancement of fluorescence was observed when citreoviridin interacted with the mitochondrial ATPase.  (+info)

Isolation of Escherichia coli mutants with an adenosine triphosphatase insensitive to aurovertin. (7/40)

Energy-transducing adenosine triphosphatase (ATPase) from Escherichia coli is inhibited by aurovertin. Aurovertin-resistant mutants were generated by nitrosoguanidine mutagenesis of E. coli AN180, whose growth on a nonfermentable carbon source was blocked by aurovertin. The ATPase activity of cell extracts from 15 different mutants (designated MA1, MA2, MA3, etc.) was found to be at least 20 times less sensitive to aurovertin than that from the parent strain. The aurovertin-resistant mutants did not show cross-resistance towards a number of ATPase inhibitors including azide, dicyclohexylcarbodiimide, quercetin, 7-chloro-4-nitrobenzofurazan, and N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. Aurovertin inhibited the energization brought about by addition of ATP to E. coli AN180 membrane vesicles; it was without effect on MA1 and MA2 membrane vesicles energized by ATP. The mutation in MA1, like other mutations of the ATPase complex, maps in the unc region of the bacterial chromosome.  (+info)

Inhibition of mitochondrial bioenergetics: the effects on structure of mitochondria in the cell and on apoptosis. (8/40)

The effects of specific inhibitors of respiratory chain, F(o)F(1)ATP synthase and uncouplers of oxidative phosphorylation on survival of carcinoma HeLa cells and on the structure of mitochondria in the cells were studied. The inhibitors of respiration (piericidin, antimycin, myxothiazol), the F(1)-component of ATP synthase (aurovertin) and uncouplers (DNP, FCCP) did not affect viability of HeLa cells, apoptosis induced by TNF or staurosporin and the anti-apoptotic action of Bcl-2. Apoptosis was induced by combined action of respiratory inhibitors and uncouplers indicating possible pro-apoptotic action of reactive oxygen species (ROS) generated by mitochondria. Short-term incubation of HeLa cells with the mitochondrial inhibitors and 2-deoxyglucose followed by 24-48 h recovery resulted in massive apoptosis. Apoptosis correlated to transient (3-4 h) and limited (60-70%) depletion of ATP. More prolonged or more complete transient ATP depletion induced pronounced necrosis. The inhibitors of respiration and uncouplers caused fragmentation of tubular mitochondria and formation of small round bodies followed by swelling. These transitions were not accompanied with release of cytochrome c into the cytosol and were fully reversible. The combined effect of respiratory inhibitors and uncouplers developed more rapidly indicating possible involvement of ROS generated by mitochondria. More prolonged (48-72 h) incubation with this combination of inhibitors caused clustering and degradation of mitochondria.  (+info)

... aurovertins MeSH D03.383.663.491 - iridoids MeSH D03.383.663.620 - nigericin MeSH D03.383.663.705 - pyran copolymer MeSH ...
Previously we introduced an automated high-dimensional non-linear registration framework, CVS, that combines volumetric and surface-based alignment to achieve robust and accurate correspondence in both cortical and sub-cortical regions (Postelnicu et al., 2009). In this paper we show that using CVS …
Aurovertins Preferred Term Term UI T003893. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1975). ... Aurovertins Preferred Concept UI. M0001976. Registry Number. 0. Scope Note. Very toxic and complex pyrone derivatives from the ... use AUROVERTINS to search AUROVERTIN 1975-79. History Note. 91(80); was see under PYRANS 1980-90; was AUROVERTIN see under ... Aurovertins. Tree Number(s). D03.383.663.075. Unique ID. D001313. RDF Unique Identifier. http://id.nlm.nih.gov/mesh/D001313 ...
Aurovertins D3.830.75 Australia Z1.338 Australian Capital Territory Z1.338.240 Z1.639.100.500 Z1.678.100.373.500 Autistic ...
Aurovertins Preferred Term Term UI T003893. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1975). ... Aurovertins Preferred Concept UI. M0001976. Registry Number. 0. Scope Note. Very toxic and complex pyrone derivatives from the ... use AUROVERTINS to search AUROVERTIN 1975-79. History Note. 91(80); was see under PYRANS 1980-90; was AUROVERTIN see under ... Aurovertins. Tree Number(s). D03.383.663.075. Unique ID. D001313. RDF Unique Identifier. http://id.nlm.nih.gov/mesh/D001313 ...
AUROVERTINS was AUROVERTIN see under ANTIBIOTICS 1975-79. DeCS ID:. 12150 Unique ID:. D011714 ...
Calcarisporium arbuscula is a mushroom endophytic fungus, which primarily produces aurovertins. Here, in an aurovertin null- ...
Auranofin N0000166667 Aurintricarboxylic Acid N0000166376 Aurodox N0000006013 Aurothioglucose N0000167172 Aurovertins ...
Aurovertins D3.830.75 Australia Z1.338 Australian Capital Territory Z1.338.240 Z1.639.100.500 Z1.678.100.373.500 Autistic ...
Aurovertins D3.830.75 Australia Z1.338 Australian Capital Territory Z1.338.240 Z1.639.100.500 Z1.678.100.373.500 Autistic ...
Aurovertins D3.830.75 Australia Z1.338 Australian Capital Territory Z1.338.240 Z1.639.100.500 Z1.678.100.373.500 Autistic ...
Aurovertins D3.830.75 Australia Z1.338 Australian Capital Territory Z1.338.240 Z1.639.100.500 Z1.678.100.373.500 Autistic ...
Aurovertins [D03.383.663.075] * Benzopyrans [D03.383.663.283] * Glaucarubin [D03.383.663.387] * Iridoids [D03.383.663.491] ...
Aurovertins Auscultation Australasia Australia Australian Capital Territory Austria Austria-Hungary Autacoids Authoritarianism ...
Aurovertins - Preferred Concept UI. M0001976. Scope note. Very toxic and complex pyrone derivatives from the fungus ... use AUROVERTINS to search AUROVERTIN 1975-79. History Note:. 91(80); was see under PYRANS 1980-90; was AUROVERTIN see under ...
... urotrygoni aurous aurovertin aurovertins aurum aurum metallicum aus aus Aus Aus AUs A. U.s A. U.s A.U.s A.U.s AUs ...
Aurovertins D3.830.75 Australia Z1.338 Australian Capital Territory Z1.338.240 Z1.639.100.500 Z1.678.100.373.500 Autistic ...
Aurovertins D3.830.75 Australia Z1.338 Australian Capital Territory Z1.338.240 Z1.639.100.500 Z1.678.100.373.500 Autistic ...
Aurovertins D3.830.75 Australia Z1.338 Australian Capital Territory Z1.338.240 Z1.639.100.500 Z1.678.100.373.500 Autistic ...

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