Aurintricarboxylic Acid: A dye which inhibits protein biosynthesis at the initial stages. The ammonium salt (aluminon) is a reagent for the colorimetric estimation of aluminum in water, foods, and tissues.Cyclohexanecarboxylic AcidsInfluenzavirus B: A genus of the family ORTHOMYXOVIRUS causing HUMAN INFLUENZA and other diseases primarily in humans. In contrast to INFLUENZAVIRUS A, no distinct antigenic subtypes of hemagglutinin (HEMAGGLUTININS) and NEURAMINIDASE are recognized.Reticulocytes: Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.Peptide Initiation Factors: Protein factors uniquely required during the initiation phase of protein synthesis in GENETIC TRANSLATION.Quaternary Ammonium Compounds: Derivatives of ammonium compounds, NH4+ Y-, in which all four of the hydrogens bonded to nitrogen have been replaced with hydrocarbyl groups. These are distinguished from IMINES which are RN=CR2.Placental Hormones: Hormones produced by the placenta include CHORIONIC GONADOTROPIN, and PLACENTAL LACTOGEN as well as steroids (ESTROGENS; PROGESTERONE), and neuropeptide hormones similar to those found in the hypothalamus (HYPOTHALAMIC HORMONES).Ribonuclease, Pancreatic: An enzyme that catalyzes the endonucleolytic cleavage of pancreatic ribonucleic acids to 3'-phosphomono- and oligonucleotides ending in cytidylic or uridylic acids with 2',3'-cyclic phosphate intermediates. EC 3.1.27.5.Ribonucleases: Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-.Education, Graduate: Studies beyond the bachelor's degree at an institution having graduate programs for the purpose of preparing for entrance into a specific field, and obtaining a higher degree.Faculty: The teaching staff and members of the administrative staff having academic rank in an educational institution.Students: Individuals enrolled in a school or formal educational program.Education, Pharmacy, Graduate: Educational programs for pharmacists who have a bachelor's degree or a Doctor of Pharmacy degree entering a specific field of pharmacy. They may lead to an advanced degree.Faculty, Medical: The teaching staff and members of the administrative staff having academic rank in a medical school.Audiovisual Aids: Auditory and visual instructional materials.Teaching: The educational process of instructing.Phosphatidylserines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.Aldehydes: Organic compounds containing a carbonyl group in the form -CHO.Oxidative Stress: A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).Cell Death: The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.Neurons: The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.von Willebrand Factor: A high-molecular-weight plasma protein, produced by endothelial cells and megakaryocytes, that is part of the factor VIII/von Willebrand factor complex. The von Willebrand factor has receptors for collagen, platelets, and ristocetin activity as well as the immunologically distinct antigenic determinants. It functions in adhesion of platelets to collagen and hemostatic plug formation. The prolonged bleeding time in VON WILLEBRAND DISEASES is due to the deficiency of this factor.Digestive System Surgical Procedures: Surgery performed on the digestive system or its parts.Research: Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)von Willebrand Diseases: Group of hemorrhagic disorders in which the VON WILLEBRAND FACTOR is either quantitatively or qualitatively abnormal. They are usually inherited as an autosomal dominant trait though rare kindreds are autosomal recessive. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII, and impaired platelet adhesion.Research Personnel: Those individuals engaged in research.Research Support as Topic: Financial support of research activities.Biomedical Research: Research that involves the application of the natural sciences, especially biology and physiology, to medicine.Neural Crest: The two longitudinal ridges along the PRIMITIVE STREAK appearing near the end of GASTRULATION during development of nervous system (NEURULATION). The ridges are formed by folding of NEURAL PLATE. Between the ridges is a neural groove which deepens as the fold become elevated. When the folds meet at midline, the groove becomes a closed tube, the NEURAL TUBE.Orthopedic Fixation Devices: Devices which are used in the treatment of orthopedic injuries and diseases.Enteric Nervous System: Two ganglionated neural plexuses in the gut wall which form one of the three major divisions of the autonomic nervous system. The enteric nervous system innervates the gastrointestinal tract, the pancreas, and the gallbladder. It contains sensory neurons, interneurons, and motor neurons. Thus the circuitry can autonomously sense the tension and the chemical environment in the gut and regulate blood vessel tone, motility, secretions, and fluid transport. The system is itself governed by the central nervous system and receives both parasympathetic and sympathetic innervation. (From Kandel, Schwartz, and Jessel, Principles of Neural Science, 3d ed, p766)Neuroblastoma: A common neoplasm of early childhood arising from neural crest cells in the sympathetic nervous system, and characterized by diverse clinical behavior, ranging from spontaneous remission to rapid metastatic progression and death. This tumor is the most common intraabdominal malignancy of childhood, but it may also arise from thorax, neck, or rarely occur in the central nervous system. Histologic features include uniform round cells with hyperchromatic nuclei arranged in nests and separated by fibrovascular septa. Neuroblastomas may be associated with the opsoclonus-myoclonus syndrome. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2099-2101; Curr Opin Oncol 1998 Jan;10(1):43-51)Antimitotic Agents: Agents that arrest cells in MITOSIS, most notably TUBULIN MODULATORS.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Volvox: A genus of GREEN ALGAE in the family Volvocaceae. They form spherical colonies of hundreds or thousands of bi-flagellated cells in a semi-transparent gelatinous ball.Biological Evolution: The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics.Caspases: A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.Caspase 3: A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.Apoptosis Regulatory Proteins: A large group of proteins that control APOPTOSIS. This family of proteins includes many ONCOGENE PROTEINS as well as a wide variety of classes of INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS such as CASPASES.Nitric Oxide Synthase Type I: A CALCIUM-dependent, constitutively-expressed form of nitric oxide synthase found primarily in NERVE TISSUE.NADP: Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed)Flavin Mononucleotide: A coenzyme for a number of oxidative enzymes including NADH DEHYDROGENASE. It is the principal form in which RIBOFLAVIN is found in cells and tissues.Protein Isoforms: Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.NADPH-Ferrihemoprotein Reductase: A flavoprotein that catalyzes the reduction of heme-thiolate-dependent monooxygenases and is part of the microsomal hydroxylating system. EC 1.6.2.4.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Aspartame: Flavoring agent sweeter than sugar, metabolized as PHENYLALANINE and ASPARTIC ACID.History, 18th Century: Time period from 1701 through 1800 of the common era.Encephalitis Virus, Murray Valley: A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE), found in Australia and New Guinea. It causes a fulminating viremia resembling Japanese encephalitis (ENCEPHALITIS, JAPANESE).Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Taste Threshold: The minimum concentration at which taste sensitivity to a particular substance or food can be perceived.New YorkSweetening Agents: Substances that sweeten food, beverages, medications, etc., such as sugar, saccharine or other low-calorie synthetic products. (From Random House Unabridged Dictionary, 2d ed)

Regeneration of renal proximal tubules after mercuric chloride injury is accompanied by increased binding of aminoacyl-transfer ribonucleic acid. (1/123)

Homogenates of rat kidney cortex obtained 1,3 or 14 days after a single injection of HgCl2 were used to prepare the post-microsomal pH5 supernatant fraction. The activity of this fraction for peptide synthesis from [14C]phenylalanyl-tRNA was significantly increased at 1 and 3 days, at which time the proximal tubules are regenerating [Cuppage & Tate (1967) Am. J. Pathol. 51, 405-429]. This increased activity could not be attributed to a decreased inhibitory activity, but was due to an increased aminoacyl-tRNA binding, i.e. elongation-factor-1 activity, in the supernatant fraction.  (+info)

Disposition of cosalane, a novel anti-HIV agent, in isolated perfused rat livers. (2/123)

Cosalane is a potent inhibitor of HIV replication with a broad range of activity. In this study, the hepatic disposition of cosalane was investigated with a noncirculating isolated perfused rat liver technique. When 6 microM cosalane was infused into livers from untreated rats, the drug was highly extracted by the liver (only 2. 5% of influent cosalane concentration appeared in the effluent perfusate). Pretreatment of rats with various inducers of cytochrome P-450 before perfusion neither altered the effluent cosalane concentration nor resulted in the appearance of detectable metabolites in the effluent perfusate or liver homogenates. Hepatic uptake of cosalane was negligible when the drug was infused in the presence of BSA, and infusion of albumin after cosalane resulted in a significant displacement of the drug into the effluent perfusate. Furthermore, permeabilization of perfused livers with digitonin significantly diminished effluent cosalane concentration while enhancing cosalane uptake by the liver. Based on our data, it appears that a significant proportion of cosalane does not penetrate the hepatocyte membrane and may accumulate in the lipid bilayer of the cell membrane. This finding supports the proposed mechanism explaining the antiviral effect of cosalane which stipulates that this compound appears to imbed perpendicularly in the lipid bilayer of the cell membrane and the viral envelope. Also, cosalane does not seem to be metabolized by the liver as evidenced by the lack of detectable metabolites in the effluent perfusate, liver homogenates, and liver microsomal incubations.  (+info)

Characterization of caspase processing and activation in HL-60 cell cytosol under cell-free conditions. Nucleotide requirement and inhibitor profile. (3/123)

The present studies compared caspase activation under cell-free conditions in vitro and in etoposide-treated HL-60 leukemia cells in situ. Immunoblotting revealed that incubation of HL-60 cytosol at 30 degrees C in the presence of cytochrome c and ATP (or dATP) resulted in activation of procaspases-3, -6, and -7 but not -2 and -8. Although similar selectivity was observed in intact cells, affinity labeling revealed that the active caspase species generated in vitro and in situ differed in charge and abundance. ATP and dATP levels in intact HL-60 cells were higher than required for caspase activation in vitro and did not change before caspase activation in situ. Replacement of ATP with the poorly hydrolyzable analogs 5'-adenylyl methylenediphosphate, 5'-adenylyl imidodiphosphate, or 5'-adenylyl-O-(3-thiotriphos-phate) slowed caspase activation in vitro, suggesting that ATP hydrolysis is required. Caspase activation in vitro was insensitive to phosphatase and kinase inhibitors (okadaic acid, staurosporine, and genistein) but was inhibited by Zn(2+), aurintricarboxylic acid, and various protease inhibitors, including 3,4-dichloroisocoumarin, N(alpha)-p-tosyl-L-phenylalanine chloromethyl ketone, N(alpha)-p-tosyl-L-lysine chloromethyl ketone, and N-(N(alpha)-benzyloxycarbonylphenylalanyl)alanine fluoromethyl ketone, each of which inhibited recombinant caspases-3, -6, -7, and -9. Experiments with anti-neoepitope antiserum confirmed that these agents inhibited caspase-9 activation. Collectively, these results suggest that caspase-9 activation requires nucleotide hydrolysis and is inhibited by agents previously thought to affect apoptosis by other means.  (+info)

Isolation of rabbit reticulocyte initiation factors by means of heparin bound to sepharose. (4/123)

Passage of cell-free extracts of rabbit reticulocytes through heparin-Sepharose affinity columns results in the loss of the ability of the effluent to initiate protein synthesis. This is shown by the loss of response to added rabbit globin mRNA or to inhibitors of initiation of protein synthesis, such as heparin and aurin tricarboxylic acid, and by recovery of initiation activity by addition of protein retained and subsequently eluted from the columns. The effluent retains, however, the ability to elongate protein chains. Only 0.8% of the applied cell extract protein binds to heparin-Sepharose columns. This bound protein, which can be recovered by increasing the salt concentration of the eluting buffer, has initiation factor activity equal to that of a crude initiation factor preparation obtained from rabbit reticulocyte ribosomes by extraction with 0.5 M KCl. The protein patterns on polyacrylamide gels of the initiation factors prepared by either method are very similar and indicate a protein mixture, which may represent a complex. These data confirm that heparin interacts specifically with initiation factos, and indicate that heparin-Sepharose chromatography will simplify procedures for the preparation of initiation factors.  (+info)

Survival function of ERK1/2 as IL-3-activated, staurosporine-resistant Bcl2 kinases. (5/123)

Bcl2 phosphorylation at Ser-70 may be required for the full and potent suppression of apoptosis in IL-3-dependent myeloid cells and can result from agonist activation of mitochondrial protein kinase C (PKC). Paradoxically, expression of exogenous Bcl2 can protect parental cells from apoptosis induced by the potent PKC inhibitor, staurosporine (stauro). High concentrations of stauro of up to 1 microM only partially inhibit IL-3-stimulated Bcl2 phosphorylation but completely block PKC-mediated Bcl2 phosphorylation in vitro. These data indicate a role for a stauro-resistant Bcl2 kinase (SRK). We show that aurintricarboxylic acid (ATA), a nonpeptide activator of cellular MEK/mitogen-activated protein kinase (MAPK) kinase, can induce Ser-70 phosphorylation of Bcl2 and support survival of cells expressing wild-type but not the phosphorylation-incompetent S70A mutant Bcl2. A role for a MEK/MAPK as a responsible SRK was implicated because the highly specific MEK/MAPK inhibitor, PD98059, also can only partially inhibit IL-3-induced Bcl2 phosphorylation, whereas the combination of PD98059 and stauro completely blocks phosphorylation and synergistically enhances apoptosis. p44MAPK/extracellular signal-regulated kinase 1 (ERK1) and p42 MAPK/ERK2 are activated by IL-3, colocalize with mitochondrial Bcl2, and can directly phosphorylate Bcl2 on Ser-70 in a stauro-resistant manner both in vitro and in vivo. These findings suggest a role for the ERK1/2 kinases as SRKs. Thus, the SRKs can serve to functionally link the IL-3-stimulated proliferative and survival signaling pathways and, in a novel capacity, may explain how Bcl2 can suppress stauro-induced apoptosis. In addition, although the mechanism of regulation of Bcl2 by phosphorylation is not yet clear, our results indicate that phosphorylation may functionally stabilize the Bcl2-Bax heterodimerization.  (+info)

Pharmacokinetics, biliary excretion, and tissue distribution of novel anti-HIV agents, cosalane and dihydrocosalane, in Sprague-Dawley rats. (6/123)

Cosalane and dihydrocosalane are potent inhibitors of HIV replication with a broad range of activity. The purpose of this study was to investigate: 1) the pharmacokinetic disposition of both cosalane and dihydrocosalane in male Sprague-Dawley rats, and 2) biliary excretion, enterohepatic circulation, and tissue distribution of cosalane after i.v. and/or oral administration. Animals were administered i.v. (10 mg/kg) cosalane or dihydrocosalane through a jugular vein to obtain plasma profiles. Dose dependence of cosalane was studied over a dose range of 1.0 to 10 mg/kg. The extent of enterohepatic recycling, biliary excretion, and tissue distribution were studied after i.v. administration. Both cosalane and dihydrocosalane exhibited a biexponential disposition with very long half-lives of 749 +/- 216 and 1016 +/- 407 min, along with very large volumes of distribution 23.1 +/- 4.4 and 24.4 +/- 2. 5 liter/kg, respectively. Both cosalane (nondetectable) and dihydrocosalane (<1%) showed very poor oral bioavailability. The biliary and renal excretions of cosalane were found to be negligible with no detectable metabolites either in urine or bile. After oral administration, more than 87% of the cosalane dose was excreted in the feces as the parent compound. Also, cosalane was sequestered significantly in liver with quantifiable levels in all tissues tested, even 48 h after the dose was administered. Therefore it was concluded that the poor oral bioavailability of cosalane may be due to its poor enterocytic transport coupled with sequestration in liver parenchymal cell membrane layers.  (+info)

Relationship between different stages of the corpus luteum and the expression of the peroxisome proliferator-activated receptor gamma protein in bovine large lutein cells. (7/123)

Lutein cells produce progestins that support pregnancy. Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism. Peroxisome proliferator-activated receptors (PPAR) are transcription factors that are central in the regulation of lipid metabolism. Hence, they may play a role in regulation of the development and regression of the corpus luteum. The present study investigated the expression of PPAR-gamma, n during different stages of the corpus luteum. Lutein cells were isolated mechanically from non-pregnant and pregnant heifers on days 5, 12 and 20 of the oestrous cycle (n = 3 for each day). PPAR-gamma in single cells was analysed by flow cytometry. PPAR-gamma 1 and PPAR-gamma 2 isoforms were distinguished by immunoblotting. The cell cycle of the lutein cells was measured by the flow cytometric quantification of DNA in single cells, using propidium iodide staining after ethanol fixation and RNAse treatment, and by the detection of the proliferating cell nuclear antigen (PCNA). The response of the cells to PPAR-gamma agonist 15-deoxy-delta 12,14 prostaglandin J2 (15dPGJ2, 200 and 490 nmol l-1) with and without changing the cell cycle by the anti-apoptogenic drug aurintricarboxylic acid (ATA, 10 mumol l-1) was used as an in vitro model to study the relationship between the cell cycle and PPAR-gamma. The concentration of PPAR-gamma per cell from non-pregnant heifers was significantly higher on day 5 (3.40 +/- 0.30 fmol) compared with that on day 12 (1.34 +/- 0.18 fmol, P < 0.05) and day 20 (0.55 +/- 0.2 fmol, P < 0.05). In pregnant heifers, the concentration of PPAR-gamma was significantly (P < 0.01) higher than in non-pregnant heifers. A decrease in the PPAR-gamma 1 isoform relative to PPAR-gamma 2 was observed in cells on day 12 of the oestrous cycle compared with day 5. The cell cycle (S phase portion in cells on days 5, 12 and 20: 16 +/- 4%, 6 +/- 4% and 4 +/- 3%, respectively) and the portion of cells with PCNA correlated with the amount of PPAR-gamma in non-pregnant heifers. ATA promoted the S phase in cells of non-pregnant heifers (day 12) and the endogenous agonist of PPAR-gamma, 15dPGJ2, inhibited the response to ATA in a dose-dependent manner, indicating that PPAR-gamma plays a role in the arrest of the cell cycle in lutein cells to maintain their differentiated state.  (+info)

VIP-derived sequences modified by N-terminal stearyl moiety induce cell death: the human keratinocyte as a model. (8/123)

Vasoactive intestinal peptide (VIP) is a recognized growth factor affecting many cell types. We have previously developed a series of lipophilic VIP analogues containing an N-terminal covalently attached stearyl moiety. The current studies identified stearyl-Nle(17)-VIP and stearyl-Nle(17)-neurotensin(6-11)VIP(7-28), acting at microM concentrations, as cytotoxic to human keratinocytes. The core C-terminal active VIP-derived peptide, stearyl-Lys-Lys-Tyr-Leu-NH(2) (St-KKYL-NH(2)), was identified as being responsible for the observed cytotoxicity. Cytotoxicity coincided with marked reduction in intracellular cyclic GMP and was abolished by co-treatment with the endonuclease inhibitor, aurine-tricarboxylic acid, suggesting apoptotic mechanisms. Stearyl-VIP derivatives thus offer lead compounds for future drug development against hyperproliferative skin conditions.  (+info)

Aurintricarboxylic acid; Aurintricarboxylic acid ammonium salt has been used as a nuclease inhibitor to study its effects on transfection.
Inclusion of aurintricarboxylic acid (ATA) in extraction buffers for the isolation of RNA from animal tissues resulted in high yields (0.5-2.0 mg/g of tissue) of undegraded material as judged by agarose-gel-electrophoretic analyses and Northern-blotting experiments. However, ATA bound to nucleic acids, forming stable complexes, and so we have established methods for spectrophotometric quantification of RNA in these coloured complexes, and for easy removal of sufficient ATA to leave RNA in a consistently hybridizable condition at the end of a purification. The use and subsequent removal of ATA was straightforward and gave satisfactory results for all rat tissues tested.. ...
The present findings demonstrate that the lipid peroxidation product HNE can induce apoptosis in PC12 cells and primary rat hippocampal neurons and suggest that HNE is a mediator of oxidative stress-induced apoptosis. HNE induced loss of plasma membrane phosphatidylserine asymmetry (detected by annexin-V binding) and nuclear condensation and DNA fragmentation in PC12 cells and hippocampal neurons. HNE-induced cell death was prevented by cycloheximide, actinomycin-D, and aurintricarboxylic acid, consistent with a role for macromolecular synthesis and endonuclease activity in the cell death process. Although we recently reported that low concentrations of cycloheximide that do not cause sustained blockade of protein synthesis can protect neurons against excitotoxic and oxidative insults by a mechanism that apparently involves stimulation of antioxidant pathways (Furukawa et al., 1997), the concentration of cycloheximide used in the present study (10 μm) causes an essentially complete and ...
Label: Independent. After the release of their critically-acclaimed album, Catharsis and their departure from Pavement Entertainment, NJ hard rock veterans Aurin went through a period of uncertainty. While plenty of new music had been recorded, and still more being written, the band entered the studio with Mike Ferretti (Sevendust, Thank You Scientist, Saliva) to record what would become their 3rd LP. However, having spent all of their funding to CREATE the album, they didnt have enough to fund a successful release. Knowing that fans wanted more, they decided to get them involved via the popular crowd-funding platform, Kickstarter. On August 2nd, the band reached their goal, Serotonins release was a certainty and it would come much sooner than anticipated. Having received the final masters back in May, they were chomping at the bit to get these songs into the spotlight.. The album kicks off with lead single, "Pause Rewind Repeat" and, in true Aurin fashion, it pulls no punches lyrically or ...
The process of cell death caused by influenza virus infection in cultured MDCK and HeLa cells was analysed. This infection gave rise to nuclear fragmentation and chromatin condensation accompanied by chromosomal DNA fragmentation into oligonucleosomes. Chromosomal DNA fragmentation progressed concomitantly with cell lysis of MDCK cells and HeLa cells, producing high and low yields of virus particles, respectively, indicating that the extent of cell lysis was not proportional to the virus production. The endonuclease inhibitor zinc blocked DNA fragmentation in MDCK cells. Cycloheximide inhibited DNA fragmentation as well as cell lysis. Inhibition occurred when the drug was added to the medium within 2 h after infection but not efficiently at 4 h or later. Infection induced the Fas Ag gene, which encodes a possible apoptosis-mediating molecule, in the early infectious stage followed by the expression of Fas Ag on the cell surface. These results suggested that influenza virus infection causes apoptotic
4M5R: Crystallographic fragment screening and structure-based optimization yields a new class of influenza endonuclease inhibitors.
Visit Healthgrades for information on Dr. Bertrand Gallet De Saint Aurin, MD Find Phone & Address information, medical practice history, affiliated hospitals and more.
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Aurin is back with their upcoming third album, Serotonin, slated for release Summer 2019 through TLG / INgrooves. Serotonin was produced by Grammy award nominated Mike Ferretti (Sevendust, Thank you Scientist, Saliva).. After their critically acclaimed 2014 release of record label debut album Catharsis on Pavement Entertainment / RED, the band hit the road, honing their live show while doing dates and touring with…. Art of Dying, Otep, Nonpoint, Icon For Hire, Gemini Syndrome, Ill Nino, Adrenaline Mob, Fit for Rivals, Smile Empty Soul, Members of Breaking Benjamin, 40 Below Summer, Islander, Tantric, Spineshank, The Bunny The Bear and more…. After bringing their new bassist Lisa Toniolo on board, they have been busy mainly behind the scenes focusing on writing, recording and preparing for the launch of Serotonin. "This album was a little different than our previous releases. We created a record that drifted a bit from our earlier and heavier music, though it was still heavily driven by our ...
Academic positions Portsmouth (UK): Head of Department / Professor at University of Portsmouth Faculty of Science, Department of Geography Full time, continuing Melbourne: City Of Melbourne Chair In Resilient Cities at the University of Melbourne Faculty of Architecture, Building and planning, worki...
Imago ķermenis ir 2,5-4,5 mm garš, to krāsa variē no tumši brūna līdz melnam; ķermenis ir ļoti apaļīgs un izliekts, gandrīz pareizs puslodes veida, nedaudz saspiests no sāniem, tā kontūras nepārtrauktas no pronotuma un segspārniem. Labrums pilnīgi nosegts zem klipeusa. Klipeuss vāji izliekts, priekšēja mala apaļīga vai pusnogriezts vidū. Acis ir sadalītas augšējā (dorsālajā) un apakšējā (ventrālajā) daļā ar galvas sānu izstiepumiem; vāji izliektas. Mezotībijas un metatībijas bez peldspalvām.[2] Galva ir nedaudz sagarināta aiz acīm. Maksilāri palpi gandrīz uz pusi tikpat gari, cik plata galva; to apikāls segments asimetrisks ar taisnāku iekšējo pusi, nedaudz garāks nekā priekšpēdējais. Labiāli palpi ir īsāki nekā mentums. Otrs segments ar subapikālu sariņu uz iekšējās vai ārējās daļas, trešais segments ir tikpat garš cik otrs, asimetrisks ar taisnāku iekšējo daļu. Gula ir labi pilnīgi attīstīta, vāji sašaurināta ...
The pH dependence of the catalytic parameters and the pattern of inhibition by the product phosphate and by other anionic compounds suggest that the active site of PEA phospho-lyase is optimized to bind dianionic groups ...
This Review article will introduce organic anion transporting polypeptides which recognize endo- and exogenous organic anionic compounds and recent findings about their upregulation in cancer cells.
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That time of the month is usually just a nuisance that we women deal with because we have to, and because were not whiny little babies like men are. But sometimes its the express train to Crampsville in Too Many Emotions Land and we just want to punch something really hard or maybe yell at someone for saying "good morning" too cheerfully. Its times like these that make us curse our lady parts and wish we didnt have all that complicated tubery going on down there. Finally, someone understands our frustration and has the perfect short-term solution: the Super Period Fun Time Uterus Piñata, available on Etsy (of course). Yes, its a piñata in the form of a uterus, only bigger!. The product description is more about loving your uterus ("because every now and again you have a period that deserves a freaking fiesta"), but if youre going to beat a uterus piñata with a stick, then its probably because youre angry. And when you finish taking out all your rage on this $140 piñata, youll be ...
PUNXSUTAWNEY - Punxsutawney Borough Council announced news that everyone has been waiting to hear for the last six years: That the Area Transportation Authority (ATA) terminal in Punxsutawney is ready to move ahead with construction within the next few weeks.. Monday, council President Susan Glessner said it has been six years since the project first began, which involved the demolition of a group of buildings along North Findley Street.. Glessner said there has been some criticism of council members because many borough residents think that nothing was ever going to happen with the site nearly two years after the buildings were demolished.. Council had partnered with ATA in order to have the terminal constructed, Glessner said, adding that the borough had agreed to have the buildings demolished and make Torrence Street a two-way street.. Glessner said that she spoke with ATA Chief Executive Mike Imbrogno, who said that the project has received final approval from its board.. ATA had to wait for ...
Muhamedi a.s., rreze drite që mposhti errësirën "Edhe të dërguarit e tjerë para teje janë përqeshur, e ata që u tallën pësuan (...
Unabh ngige, standardisierte Informationen ber Old Dutch Genetics Cannabis-Strain Ata Tundra! Kommentare, Bl te-Zeit, THC-Gehalt, Bilder, Preisvergleich & Shops, Stammbaum, Kreuzungen & Hybriden, Growreports, Ph notypen, Sorten-Vergleiche, medizinische Eigenschaften und vieles mehr!
A small peach colored vagina piñata. Measures 10 x 15 x 4. *Some Vagina Piñatas are slightly different than others, thats just life! (This is a handmade item and may have slight variations in color, placement of materials, etc.)
Ata Sikka ETHNONYMS: Ata Biang, Ata Krowé, Sika, Sikka, Sikkanese Source for information on Ata Sikka: Encyclopedia of World Cultures dictionary.
Read the Piñata: Survival Island movie synopsis, view the movie trailer, get cast and crew information, see movie photos, and more on Movies.com.
If this is your first visit, be sure to check out the FAQ by clicking the link above. You may have to register before you can post: click the register link above to proceed. To start viewing messages, select the forum that you want to visit from the selection below ...
Mehmet Uzun1, Eralp Ulusoy2, Oben Baysan1, Mehmet Yoku o lu1, Fethi K l aslan2, Bilgehan Sava z3, Ata K r lmaz2, Cem K z1, Celal Gen 1, Hayrettin Karaeren1, Mehmet Din t rk2, Ersoy I k1 ...
... nda Kanıta Dayalı Yaklaşım Bülent Urman - Ayşe Seyhan Ata TSRM - Üreme Sağlığı ve İnfertilite Derneği Tekrarlayan gebelik kaybı(TGK), 20. gebelik haftasından
Buy a ProX 2U Space Amp Rack Mount ATA Flight Case, 19" Depth [T-2RSS] at idjnow. Order online or call (800) 355-7746. Most orders ship free.
Its a piñata...shaped like a uterus. So wrong its right? I cant decide. Says Etsy seller AmieKin, Its great for baby showers, or Red Tent...
Eni Paulo, nexi noxou Timoti ta mitema sou xaixai noxou Kalisito Iesu, tekaukavunu vaivaikala ane o ulai noxinge ngingi ta mitema ane Kalisito Iesu ta nau mo ta tatila sou isaxilainge iloa ta mitema sou xaixai no a lalotu ne Pilipai.
Hrozná trojčata. To je překlad názvu této on-line hry pro dívky. Sestřička Adéla je už z trojčat, o které se musí starat unavená. Chce si zdřímnout.
Ko je naša sodelavka Eva, mamica dveh otrok, hujšala iz dneva v dan, smo jo vprašali po njeni skrivnosti. Pokazala nam je izdelek, ki ga priporočata Dr. Oz in Oprah in je spremenil življenja na tisoče Slovencev. Preberite zgodbo o ODKRITJU STOLETJA.
Seiji Takasugi obtained a jewel called "Aurin" which is the earths alter ego and it has elected him to be the commander of eight brave robots. The Brave robots are sleeping in many parts of the world as "Brave Stones". However, they become super robots by fusing with modern people and machines once Seiji awakens them and they soon follow his instructions. Their enemy is Lord OhBoss who is an invader who aims at stealing the planets energy with the aid of his subordinates that Da-Garn and the other brave robots must stop in order to save the world.. ...
Explorations of the therapeutic potential of heparin mimetics, anionic compounds that are analogues of glycosaminoglycans (GAGs), have gone hand-in-hand with the emergence of understanding as to the role of GAGs in many essential biological processes. A myriad of structurally different heparin mimetics have been prepared and examined in many diverse applications. They range in complexity from heterogeneous polysaccharides that have been chemically sulphated to well-defined compounds, designed in part to mimic the natural ligand, but with binding specificity and potency increased by conjugation to non-carbohydrate pharmacophores. The maturity of the field is illustrated by the seven heparin mimetics that have achieved marketing approval and there are several more in late-stage clinical development. An overview of the structural determinants of heparin mimetics is presented together with an indication of their activities. The challenges in developing heparin mimetics as drugs, specificity and ...
Ata were parsed from the set of PDB files available as of November 2012. Chains were counted rather than PDB entries as expression information is recorded by
Salve, vi espongo il mio quesito: attualmente ho montato un hdd da 200Gb ATA (o IDE, o EIDE, come preferite); ora, ne vorrei montare unaltro, le dimensione più o meno saranno le stesse, solo che non so se prenderlo di nuovo ATA oppure SATA; sulla carta questultimo è migliore, confermate ? vorrei solo sapere se è possibile avere contemporaneamente un hdd ATA ed uno SATA. è una banalità, ma è sempre meglio non rischiare. ciao ah, dimenticavo, quale marca secondo voi è la migliore ? attualmente il mio hdd è un Maxtor ...
Features: Nearly indestructible lightweight polyethylene construction Angled pad & Velcro wedge to hold mixer securely Tricot-lined foam interior Completely
Platt Cases, aka Platt Luggage, is a manufacturer of professional and tool cases for business and industry since 1921. Platt offers over 350 standard cases and an infinite variety of custom cases. Platt specializes in custom molded, sewn, and ATA fabricated cases, as well as custom foam inserts and tool pallets.
Stresi, dietë të varfër dhe ekologji e keqe dhe shumë faktorë të tjerë, duke përfshirë infeksionit dhe aborteve të kaluarën shpesh shkaktojnë përçarje të vezoreve. Sipas burimeve të ndryshme, ata...
Informacije o datotekah. Velikost datoteke za prenos in potreben prostor v napravi ali na kartici microSD™/SD™ znašata 780 MB. Če v napravi ni dovolj prostora za preneseno datoteko, lahko podatke shranite na kartico microSD/SD (za programiranje podatkov zemljevidov lahko uporabljate obnovljene kartice microSD).. ...
7594 7603 7612 7621 7630 7639 5 ATG GCA CAT GCA GCG CAA GTA GGT CTA CAA GAC GCT ACT TCC CCT ATC ATA GAA --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- M A H A A Q V G L Q D A T S P I M E 7648 7657 7666 7675 7684 7693 GAG CTT ATC ACC TTT CAT GAT CAC GCC CTC ATA ATC ATT TTC CTT ATC TGC TTC --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- E L I T F H D H A L M I I F L I C F 7702 7711 7720 7729 7738 7747 CTA GTC CTG TAT GCC CTT TTC CTA ACA CTC ACA ACA AAA CTA ACT AAT ACT AAC --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- L V L Y A L F L T L T T K L T N T N 7756 7765 7774 7783 7792 7801 ATC TCA GAC GCT CAG GAA ATA GAA ACC GTC TGA ACT ATC CTG CCC GCC ATC ATC --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- I S D A Q E M E T V W T I L P A I I 7810 7819 7828 7837 7846 7855 CTA GTC CTC ATC GCC CTC CCA TCC CTA CGC ATC CTT TAC ATA ACA GAC GAG GTC --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- ...
AMERICAN THYROID ASSOCIATION® , ATA® , THYROID® , CLINICAL THYROIDOLOGY® , and the distinctive circular logo are registered in the U.S. Patent and Trademark Office as trademarks of the American Thyroid Association® , Inc. ...
Buy Marathon MA-DNX300 Flight Road ATA Mixer Case features Fits Denon DNX300 Mixer, Fits Other 10 Mixers. Review Marathon Mixer & Controller Cases, Cases
The new Jackknife from S4Gear is pretty fun! Ive used it on three hunts late season in MN. Shot two deer and got both on video using my smartphone. Then using my computer I made my own vids to share and post online. Also texted the shot right from my stand to my friends! They all thought it was cool. Gotta luv technology!. ...
Adi Kriegisch wrote: , Hi Sam! , , Thank you for your quick reply! , ,, Just for sanity, what version of vblade are you using? I didnt think ,, I broke anything with the latest release ... , I first tried with v13; yesterday I repeated all the tests with v14 with , exactly the same results (and though decided to ask for help). , Module aoe is version 39 running in a stock debian sid kernel with: , vermagic: 2.6.18-2-xen-686 SMP mod_unload 686 REGPARM gcc-4.1 , , [SNIP] ,,, This is reproduceable in both directions. The funny thing is that it ,,, works like a charm via loopback on the same server. ,, The output of aoe-stat would be helpful to ensure you can actually see ,, what you think you can see. Also, if you , output of aoe-stat (I always checked that and just left it out in my posting , to not make it too long and unreadable): , e0.0 5.009GB eth0 up , At the beginning I did the testing just with killing vblade and removing and , reinserting module aoe. After some while I decided to reboot ...
Do you already have test personnel and equipment, but lack the experience to efficiently run a successful test program? If you need training or engineering…
Hello, Im running edgy ubuntu on my dual core laptop with the smp kernel, and every now and then, my computer freezes up temporarily before recoverin
PORTALI RROKUM.TV ASHT PJESE E KOMPANISE JAVA MULTIMEDIA PRODUCTION (JMP) DHE OPERON MBRENDA TELEVIZIONIT RROKUM DHE PERGJEGJESISE EDITORIALE TE TELEVIZIONIT. TANA TE DREJTAT E PERMBAJTJES SE PUBLIKUEME JANE TE REZERVUEME DHE PRONE E JMP-RROKUM. REZERVOHET E DREJTA E REDAKTIMIT DHE MOSPUBLIKIMIT TE KOMENTEVE DHE OPINIONEVE QE BIEN NDESH ME KODEKSIN E GAZETARISE DHE POLITIKAT EDITORIALE TE TELEVIZIONIT RROKUM ...
For our favors we had small bags filled with some goodies and toys. They were just partially filled as we had a piñata and wanted each kid to have their own bag to fill with the goodies from that as well ...
(PZ) ATA Airlines Reports Record September Load Factor ATA Airlines today reported that September year over year scheduled service passenger load factor increased 3.6 points to 74.5 percent. Year-to-date, ATAs load factor is up 10.2 points from 2005. September scheduled service traffic decreased 37.6 percent from 2005 to 298.0 million RPMs (revenue passenger miles). Entering its 33rd year of operations, ATA offers affordable travel from destinations like Guadalajara, Cancun, Hawaii, Oakland, Chicago, New York, Dallas/Ft. Through direct and connecting Southwest Airlines codeshare flights, ATA now serves customers in more than 60 markets. ATA provides features travelers have come to expect, including advanced seat assignments, self-service kiosks, Web check-in, and a frequent flier program with one of the lowest thresholds for earning travel in the industry. ATA Travel Awards members have the option when booking their flights on ata.com or at 1-800-I-FLY-ATA of earning either ATA Travel Awards
Bu çalışmada; kanserli çocuk hastalarda antikanser tedavinin infeksiyon hastalıklarında prokalsitonin ve C-reaktif proteinin tanısal değerleri üzerindeki etkisi araştırılmıştır. Çalışmamızda, antikanser tedavi alan ve ateş nedeniyle izlenen 63 kanserli çocuk hastanın 117 ateş atağı geriye dönük olarak değerlendirildi. Kontrol grubunda ise immün yetmezliği olmayan ateş ve infeksiyon nedeniyle yatarak tedavi görmüş 116 çocuk hastada görülen 117 ateş atağı değerlendirildi. Hastaların ateş başlangıcındaki laboratuvar bulguları, prokalsitonin ve C-reaktif protein değerleri, ateş odakları, kateter varlığı, kültür sonuçları, kullanılan antibiyotikler ve intravenöz antibiyotik tedavi süreleri incelendi. Antikanser tedavi alan ve ateş nedeniyle takip edilen hastalarda hemoglobin düzeyi, beyaz küre, trombosit ve mutlak nötrofil sayısı ortalama değerleri kontrol grubuna göre düşüktü (p ,0,001). Ateşli gün sayısı ölçeğinde ...
Can you name the 2008 Jeopardy Contestant Test? Test your knowledge on this miscellaneous quiz to see how you do and compare your score to others. Quiz by jimpoz
Low molecular weight components of the aurintricarboxylic acid complex have been shown to be non-toxic and orally effective. " ... In August 2012, McGeer and his wife Edith founded Aurin Biotech Inc., following indications that the Aurintricarboxylic acid ( ...
doi:10.1016/B978-0-444-63929-5.00002-4. Benchokroun, Y; Couprie, J; Larsen, AK (1995). "Aurintricarboxylic acid, a putative ... aurintricarboxylic acid, and HU-331, a quinolone synthesized from cannabidiol. Numerous plant derived natural phenols (ex. EGCG ... Quinolones (including nalidixic acid and ciprofloxacin) have this function. Quinolones bind to these enzymes and prevent them ...
... , the triammonium salt of aurintricarboxylic acid, is a dye commonly used to detect the presence of the aluminium ion ... Aluminon is prepared by reacting sodium nitrite with salicylic acid, adding formaldehyde, then treating with ammonia. ...
"Aurintricarboxylic acid". Sigma-Aldrich. "Aurintricarboxylic Acid". Reference.MD. Skidmore, A. F.; Beebee, T. J. (October 1989 ... Aurintricarboxylic acid can be prepared by the condensation of formaldehyde with salicylic acid in the presence of nitrite- ... Aurintricarboxylic acid (ATA) is a chemical compound that readily polymerizes in aqueous solution, forming a stable free ... Hashem, A. M.; Flaman, A. S.; Farnsworth, A.; Brown, E. G.; van Domselaar, G.; He, R.; Li, X. (2009). "Aurintricarboxylic Acid ...
... abscisic acid MeSH D02.241.223.268.070 --- aurintricarboxylic acid MeSH D02.241.223.268.220 --- chlorogenic acid MeSH D02.241. ... edetic acid MeSH D02.241.081.038.455 --- egtazic acid MeSH D02.241.081.038.581 --- iodoacetic acid MeSH D02.241.081.038.581.400 ... muramic acids MeSH D02.241.081.844.562 --- neuraminic acids MeSH D02.241.081.844.562.668 --- sialic acids MeSH D02.241.081.844. ... quinic acid MeSH D02.241.511.852 --- shikimic acid MeSH D02.241.511.902 --- sugar acids MeSH D02.241.511.902.107 --- ascorbic ...
Aurintricarboxylic acid ammonium salt has been used as a nuclease inhibitor to study its effects on transfection. ... Aurintricarboxylic acid ammonium salt has been used as a nuclease inhibitor to study its effects on transfection. ... Aurintricarboxylic acid inhibits apoptosis in many cell types. Its neuroprotective effect, perhaps due to its ability to ... Aurintricarboxylic acid readily polymerizes in aqueous solution, forming a stable free radical that inhibits protein-nucleic ...
Inclusion of aurintricarboxylic acid (ATA) in extraction buffers for the isolation of RNA from animal tissues resulted in high ... Characterization and use of the potent ribonuclease inhibitor aurintricarboxylic acid for the isolation of RNA from animal ... Characterization and use of the potent ribonuclease inhibitor aurintricarboxylic acid for the isolation of RNA from animal ... Characterization and use of the potent ribonuclease inhibitor aurintricarboxylic acid for the isolation of RNA from animal ...
... pgegen at rnaworld.bio.ukans.edu pgegen at rnaworld.bio.ukans.edu Tue Nov 7 11:05:02 EST 1995 * ... The extraction buffer ,includes Aurintricarboxylic Acid (ATA). The final total RNA pellet is ,pink. The red book says that this ... Thanks in advance for your help ATA will inhibit most enzymes that bind to nucleic acids; thats why its so effective as a ...
"Aurintricarboxylic acid". Sigma-Aldrich. "Aurintricarboxylic Acid". Reference.MD. Skidmore, A. F.; Beebee, T. J. (October 1989 ... Aurintricarboxylic acid can be prepared by the condensation of formaldehyde with salicylic acid in the presence of nitrite- ... Aurintricarboxylic acid (ATA) is a chemical compound that readily polymerizes in aqueous solution, forming a stable free ... Hashem, A. M.; Flaman, A. S.; Farnsworth, A.; Brown, E. G.; van Domselaar, G.; He, R.; Li, X. (2009). "Aurintricarboxylic Acid ...
In this study, we evaluated ATA as a potential antiviral drug against ZIKV replication. The antiviral activity of ATA against ZIKV replication in vitro showed median inhibitory concentrations (IC50) of 13.87 ± 1.09 μM and 33.33 ± 1.13 μM in Vero and A549 cells, respectively; without showing any cytotoxic effect in both cell lines (median cytotoxic concentration (CC50)| 1,000...
The Inhibitory Effect of Aurintricarboxylic Acid on the Attachment of Ribosomes to Microsomal Membranes in Vitro. ELENA ... The Inhibitory Effect of Aurintricarboxylic Acid on the Attachment of Ribosomes to Microsomal Membranes in Vitro. ELENA ... The Inhibitory Effect of Aurintricarboxylic Acid on the Attachment of Ribosomes to Microsomal Membranes in Vitro. ELENA ... Aurintricarboxylic acid was shown to inhibit the binding of ribosomes to rough microsomal membranes stripped of their ribosomes ...
Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation. Nucleic Acids Res . 1977; 4: 3055- ... Aurintricarboxylic acid is an inhibitor of mu- and m-calpain. Biochem Mol Biol Int . 1995; 36: 291-299. [PubMed] ... Aurintricarboxylic Acid (ATA) is a small molecule inhibitor of MAC. Our hypothesis was that attenuation of the formation of MAC ... Aurin tricarboxylic acid self-protects by inhibiting aberrant complement activation at the C3 convertase and C9 binding stages ...
Aurintricarboxylic Acid. £113.00 SKU:. 40000020-1. Size:. 1 g. Suppl:. bioWORLD. Add to order Added to order View basket ...
4-Cyclohexadiene-1-carboxylic acid, 3-(bis(3-carboxy-4-hydroxyphenyl)methylene)-6-oxo-, triammonium salt including: 12 synonyms ... Aurintricarboxylic Acid*Aurintricarboxylic acid ammonium salt*Aurintricarboxylic acid triammonium salt*Benzoic acid, 5-((3- ... 1,4-Cyclohexadiene-1-carboxylic acid, 3-(bis(3-carboxy-4-hydroxyphenyl) methylene)-6-oxo-, triammonium salt. Identifications. * ... 1,4-Cyclohexadiene-1-carboxylic acid, 3-(bis(3-carboxy-4-hydroxyphenyl) methylene)-6-oxo-, triammonium salt*Aluminon*Ammonium ...
Potent Inhibition of Zika Virus Replication by Aurintricarboxylic Acid.. Park JG, Ávila-Pérez G, Madere F, Hilimire TA, Nogales ... Dual Recognition of Sialic Acid and αGal Epitopes by the VP8* Domains of the Bovine Rotavirus G6P[5] WC3 and of Its Mono- ... Identification and Semi-Synthesis of 3-O-Protocatechuoylceanothic Acid, a Novel and Natural GPR120 Agonist †. ...
... all were significantly lessened by aurintricarboxylic acid. Gel electrophoresis showed no increase in DNA fragmentation over ... Both alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid and kainic acid caused vacuolation, dilatation of the ... encodes a protein in which the deduced carboxyterminal three amino acids are replaced with a unique 21 amino acid stretch ... Under these acid conditions, N-methyl-D-aspartate and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate-kainate antagonists ...
Ascorbic Acid / pharmacology. Aurintricarboxylic Acid / pharmacology. Blood. Calcium Channels / drug effects. Cell ... 4431-00-9/Aurintricarboxylic Acid; 50-81-7/Ascorbic Acid; 60-92-4/Cyclic AMP; 7447-40-7/Potassium Chloride ... However, agents known to prevent neuronal programmed cell death (cyclic AMP, KCl, aurintricarboxylic acid, and cycloheximide) ...
Mice were pretreated with acetylsalicylic acid (ASA) (n = 4), apyrase (n = 6), aurintricarboxylic acid (ATA) (n = 3), alteplase ... 1F). Furthermore, the pharmacological blockade of von Willebrand factor (vWF) binding to GPIb (ATA; aurintricarboxylic acid) or ... FcγRIIATGN mice pretreated with aurintricarboxylic acid (ATA) or diluent (G; n = 8); and FcγRIIATGN mice pretreated with ...
1995) Aurintricarboxylic acid is an inhibitor of mu- and m-calpain. Biochem Mol Biol Int 36:291-299. ... 1991) Aurintricarboxylic acid rescues PC12 cells and sympathetic neurons from cell death caused by nerve growth factor ... HNE-induced cell death was prevented by cycloheximide, actinomycin-D, and aurintricarboxylic acid, consistent with a role for ... 1995) Excitotoxicity at both NMDA and non-NMDA glutamate receptors is antagonized by aurintricarboxylic acid: evidence for ...
aurintricarboxylic acid. ZT. zeitgeber time. GAPDH. glyceraldehyde-3-phosphate dehydrogenase. RT-PCR. reverse transcription- ... 1990) Aurintricarboxylic acid, the anti-AIDS compound, prevents the binding of interferon-alpha to its receptor. Biochem ... Aurintricarboxylic acid, a ligand inhibitor of IFN-α, dose dependently inhibited the IFN-α-induced phosphorylation of the ... Aurintricarboxylic acid (ATA) was purchased from Sigma Chemical (St. Louis, MO) and solubilized in dimethyl sulfoxide. ...
The effects of aurintricarboxylic acid, an endonuclease inhibitor, were studied as well. No protection against glutamate ...
and Aurin tricarboxylic acid (ATA) is known to inhibit ristocetin-induced platelet aggregation but not the other platelet ... Publications] J.P Girma: Aurin tricarboxylic acid abolishes von Willebrand factor.Mediated platelet adhesion to collagen by ... Publications] J. P. Girma: Aurin tricarboxylic acid abolishes von Willebrand factor. Mediated platelet adhesion to collagen by ... That is, The epitope was localized between the amino acid residue 911 and 1365.. II. The analysis of plasma von Willebrand ...
The Effects of Aurintricarboxylic Acid in CHO Cultures in Chemically Defined Elaine Lee ... Using Peptide Nucleic Acid Capture Probes to Identify 16s rRNA Gene of Sepsis causing Bacteria in Whole Blood Specimens ...
Potent Inhibition of Zika Virus Replication by Aurintricarboxylic Acid. In this study, we evaluated ATA as a potential ...
Potent Inhibition of Zika Virus Replication by Aurintricarboxylic Acid. In this study, we evaluated ATA as a potential ...
Potent Inhibition of Zika Virus Replication by Aurintricarboxylic Acid. In this study, we evaluated ATA as a potential ... at the N-terminus of the HY12-encoded 3A protein at amino acid positions 2, 3, and 9. The recombinant HY12 viruses expressing ...
Potent Inhibition of Zika Virus Replication by Aurintricarboxylic Acid. In this study, we evaluated ATA as a potential ...
"Aurin tricarboxylic acid displayed about 80 percent inhibition of AIF-DNA binding," Hergenrother said. "Aurin tricarboxylic ... and through this screen aurin tricarboxylic acid was identified as the first in vitro inhibitor of AIF. " ... acid was the only compound to exhibit significant inhibition out of approximately 1000 compounds screened." While the photonic ...
Aurintricarboxylic Acid. ATA. Auckland Transition Agency (New Zealand). ATA. Atmosphere Absolute. ATA. Automatic Temperature ...
Doppler flowmetry showed that aurintricarboxylic acid was effective in restoring vessel patency after occlusive thrombosis in ... aurintricarboxylic acid, which blocks the GPIbα binding domain of vWF, was injected 15 minutes after formation of a FeCl3- ... with aurintricarboxylic acid (ATA). *P,0.05 vs Wistar rats; †P,0.05 vs SHR before ATA. ... aurintricarboxylic acid did not rescue blood flow in Wistar carotid arteries. ...
  • Aurintricarboxylic acid was shown to inhibit the binding of ribosomes to rough microsomal membranes stripped of their ribosomes in a cell-free system derived from rat liver. (aspetjournals.org)
  • and Aurin tricarboxylic acid (ATA) is known to inhibit ristocetin-induced platelet aggregation but not the other platelet aggregaters. (nii.ac.jp)
  • In August 2012, McGeer and his wife Edith founded Aurin Biotech Inc., following indications that the Aurintricarboxylic acid (ATA) complex inhibit activation of the Complement system. (wikipedia.org)
  • The previously unrecognized ability of aurintricarboxylic acid to inhibit PDGFR signaling, discovered through a screen of 1,739 compounds, demonstrates the feasibility and generalizability of GE-HTS for the discovery of small molecule modulators of any signaling pathway of interest. (broadinstitute.org)
  • We found that Evans Blue and aurin tricarboxylic acid (ATA) could completely inhibit binding of anti-CD4 (Leu 3a) and rgp120 and have selectivity for the CD4 receptor. (springer.com)
  • Balzarini J, Mitsuya H, De Clercq E and Broder S, Aurintricarboxylic acid and Evans Blue represent two different classes of anionic compounds which selectively inhibit the cytopathogenicity of human T-cell lymphotropic virus type III/lymphadenopathy-associated virus. (springer.com)
  • Inhibitors of the initiation of polypeptide synthesis, e.g., potassium ions, 7-methylguanosine 5′ -monophosphate, and aurintricarboxylic acid, were shown not only to inhibit overall BMV protein synthesis but also to change the ratio of BMV polypeptides synthesized. (asm.org)
  • The neurotoxic effects of beta-N-methylamino-L-alanine were blocked by D(-)-2-amino-5-phosphonopentanoic acid, and those of beta-N-oxalylamino-L-alanine were blocked by kynurenic acid, which demonstrated that these excitotoxins caused cerebellar granule cell death through the activation of glutamate receptors. (biology-online.org)
  • Inhibition of integrin αDβ2-mediated macrophage adhesion to end product of docosahexaenoic acid (DHA) oxidation prevents macrophage accumulation during inflammation. (peprotech.com)
  • A putative protein encoded by the clone NTCP-23 had an amino acid sequence with significant similarities to those of plant senescence-associated cysteine proteases and mammalian cathepsin H. Northern blot analysis showed that NTCP-23 mRNA is expressed in all organs and the mRNA and protein expression is enhanced during natural senescence. (deepdyve.com)
  • The availability of methods for the purification of high quality ribonucleic acids (RNA) is essential for studies aimed at constructing cDNA libraries or performing array-based hybridisations where microgram quantities of concentrated and chemically pure mRNA may be required. (biomedcentral.com)
  • The use of site-directed mutagenesis to produce NGF species modified at specific amino acid residues has facilitated identification of those residues critical for binding of NGF to either trkA or p75. (jneurosci.org)
  • Besides the evidence of direct cytotoxic consequences leading to RGC death, it also seems highly possible that ROS are involved in signaling RGC death by acting as a second messenger and/or modulating protein function by redox modifications of downstream effectors through enzymatic oxidation of specific amino acid residues. (deepdyve.com)
  • In the presence of ATP, cytosol and fully active proteasomes, CFTR was rapidly degraded and released into the cytosol solely in the form of trichloroacetic acid (TCA)-soluble peptide fragments. (biologists.org)
  • Cushman M, Kanamathareddy S, De Clercq E, Schols D, Goldman ME and Bowen JA, Synthesis and anti-HIV activities of low molecural weight aurintricarboxylic acid fragments and related compounds. (springer.com)
  • Evidence of SARS CoV replication in the lungs and intestine is provided by detection of virus particles through electron microscopy, by virus isolation, and by the detection of viral antigens and nucleic acid through immunohistology and in situ hybridization. (asmscience.org)
  • citation needed] Aurintricarboxylic acid can be prepared by the condensation of formaldehyde with salicylic acid in the presence of nitrite-containing sulfuric acid. (wikipedia.org)
  • three temperature-sensitive (ts) mutants of foot-and-mouth disease virus were classified as ribonucleic acid negative and as belonging to the same complementation group when measured by virus yields and 3h uridine incorporation in paired, mixed infections at the nonpermissive temperature (38.5c). mutant ts-22, the only mutant able to produce plaques at 38.5 c, was more sensitive to acid than were the parental wild-type or other mutant viruses. (liverpool.ac.uk)
  • Aurintricarboxylic Acid (ATA) is a chemical compound that in laboratory tests was shown to block the chemical cascade that otherwise allows glioblastoma cells to invade normal brain tissue and resist both chemo and radiation therapy, according to a TGen-led report published today in the scientific journal Oncotarget . (cityofhope.org)
  • A study led by scientists at the Translational Genomics Research Institute (TGen) has identified a chemical compound called Aurintricarboxylic Acid (ATA), shown to block the chemical cascade that otherwise allows glioblastoma cells to resist both chemo and radiation therapy. (cityofhope.org)
  • Identification of aurintricarboxylic acid as a potent allosteric antagonist of P2X1 and P2X3 receptors. (ukaachen.de)
  • Inclusion of aurintricarboxylic acid (ATA) in extraction buffers for the isolation of RNA from animal tissues resulted in high yields (0.5-2.0 mg/g of tissue) of undegraded material as judged by agarose-gel-electrophoretic analyses and Northern-blotting experiments. (biochemj.org)
  • However, the main evidence that this is the case was the finding that isolated nuclei incorporated radiolabelled amino acids into nascent peptides and this was countered by arguments that those isolated nuclei were contaminated with cytoplasmic ribosomes. (biologists.org)
  • The deduced amino acid sequences of the five open reading frames are highly conserved among A. actinomycetemcomitans strains 652, Y4, 29522, and HK1651. (jimmunol.org)
  • Compositions for oral administration of zoledronic acid or related compounds for treating complex regional pain. (patents.com)
  • This invention relates to nucleic acid hybridization assay methods and reagent systems for detecting specific polynucleotide sequences. (google.ca)
  • The principle of nucleic acid hybridization assays was developed by workers in the recombinant DNA field as a means for determining and isolating particular polynucleotide base sequences of interest. (google.ca)
  • Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. (google.ca)
  • A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or RNA.RNA hybrids with the particular polynucleotide sequence to be determined. (google.ca)
  • No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution. (google.ca)
  • This novel isoform is slightly larger than the neuronal nNOSα because of an alternative splicing that inserts a 34 amino acid peptide into the protein sequence between the CaM- and FMN-binding domains of the protein. (aspetjournals.org)
  • Over-activation of AMPARs (α−amino-3-hydroxy-5-methylisoxazole-4-propionic acid subtype glutamate receptors) is implicated in excitotoxic neuronal death associated with acute brain insults, such as ischemic stroke. (biomedcentral.com)
  • Four of these inhibitors, rifamycin AF/013, o-phenanthroline, aurintricarboxylic acid and pyridoxal 5-phosphate appear to block directly binding of the activated receptor complex to the above "acceptors. (springer.com)
  • Aluminium deposits were detected in eight canine fibrosarcomas from presumed injection sites and 11 feline post-vaccinal fibrosarcomas by the aurintricarboxylic acid method. (cavaliertalk.com)
  • By labeling such complementary probe nucleic acids with some readily detectable chemical group, it was then made possible to detect the presence of any polynucleotide sequence of interest in a test medium containing sample nucleic acids in single stranded form. (google.ca)
  • After 15 seconds add 0.5 µl aurintricarboxylic acid to 75 µM final concentration to synchronize the reaction5, and incubate further at 30 °C. Alternatively, the translation reaction can be performed from 24 °C- 37 °C if necessary, however the optimal translation temperature for RTS100 E. coli HY (suggested also by the manufacturer) is 30 °C. (scienceexchange.com)
  • AbstractFull-length infectious cDNA clones for recombinant HY12 bovine enteroviruses designated as rHY12-3A-2-HA, rHY12-3A-3-HA, and rHY12-3A-9-HA were constructed by the insertion of an epitope from influenza virus hemagglutinin (HA) at the N-terminus of the HY12-encoded 3A protein at amino acid positions 2, 3, and 9. (medworm.com)
  • Low molecular weight components of the aurintricarboxylic acid complex have been shown to be non-toxic and orally effective. (wikipedia.org)