Attachment Sites, Microbiological
Lysogeny
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Bile Pigments
Amino Acid Sequence
Integrases
Base Sequence
Virus Attachment
Musculoskeletal Development
Binding Sites
Phycoerythrin
Bacteriophage lambda
Nuclear Matrix
The residual framework structure of the CELL NUCLEUS that maintains many of the overall architectural features of the cell nucleus including the nuclear lamina with NUCLEAR PORE complex structures, residual CELL NUCLEOLI and an extensive fibrogranular structure in the nuclear interior. (Advan. Enzyme Regul. 2002; 42:39-52)
Chromosomes, Bacterial
Recombination, Genetic
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Reactive Attachment Disorder
Markedly disturbed and developmentally inappropriate social relatedness that begins before age 5 and is associated with grossly pathological child care. The child may persistently fail to initiate and respond to social interactions in a developmentally appropriate way (inhibited type) or there may be a pattern of diffuse attachments with nondiscriminate sociability (disinhibited type). (From DSM-V)
Tendons
Kinetochores
Virus Integration
Glycosylation
Bacterial Adhesion
Siphoviridae
Mutation
Glycosylphosphatidylinositols
Cloning, Molecular
DNA Nucleotidyltransferases
Plasmids
Protein Binding
Receptors, Virus
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Fibronectins
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
Cell Membrane
Corynebacterium diphtheriae
A species of gram-positive, asporogenous bacteria in which three cultural types are recognized. These types (gravis, intermedius, and mitis) were originally given in accordance with the clinical severity of the cases from which the different strains were most frequently isolated. This species is the causative agent of DIPHTHERIA.
Rhodophyta
Plants of the division Rhodophyta, commonly known as red algae, in which the red pigment (PHYCOERYTHRIN) predominates. However, if this pigment is destroyed, the algae can appear purple, brown, green, or yellow. Two important substances found in the cell walls of red algae are AGAR and CARRAGEENAN. Some rhodophyta are notable SEAWEED (macroalgae).
Heparitin Sulfate
Sequence Homology, Amino Acid
Salmonella Phages
Microtubules
Transduction, Genetic
Restriction Mapping
Integrins
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
Membrane Proteins
Chondroitin Sulfates
Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.
Peptide Fragments
Matrix Attachment Regions
Regions of the CHROMATIN or DNA that bind to the NUCLEAR MATRIX. They are found in INTERGENIC DNA, especially flanking the 5' ends of genes or clusters of genes. Many of the regions that have been isolated contain a bipartite sequence motif called the MAR/SAR recognition signature sequence that binds to MATRIX ATTACHMENT REGION BINDING PROTEINS.
Glycopeptides
Prophages
Ligaments
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Glycosaminoglycans
Microscopy, Electron, Scanning
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Trypsin
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Drosophila Proteins
Periodontal Attachment Loss
Loss or destruction of periodontal tissue caused by periodontitis or other destructive periodontal diseases or by injury during instrumentation. Attachment refers to the periodontal ligament which attaches to the alveolar bone. It has been hypothesized that treatment of the underlying periodontal disease and the seeding of periodontal ligament cells enable the creating of new attachment.
Extracellular Matrix Proteins
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
Chromosome Mapping
Glycoproteins
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Pantetheine
Drosophila
Cricetinae
Laminin
Syndecans
A family of transmembrane glycoproteins that contain a short cytoplasmic domain, a single-span transmembrane domain, and an extracellular domain with heparin sulfate and CHONDROITIN SULFATE chains. Syndecans interact with a variety of heparin-binding INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS and may play a role in modulating cellular signaling during EMBRYONIC DEVELOPMENT, tumorigenesis, and angiogenesis.
Cells, Cultured
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Sequence Homology, Nucleic Acid
Heparan Sulfate Proteoglycans
Ubiquitous macromolecules associated with the cell surface and extracellular matrix of a wide range of cells of vertebrate and invertebrate tissues. They are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (From Cancer Metastasis Rev 1996; 15(2): 177-86; Hepatology 1996; 24(3): 524-32)
Bacteriophage P2
Recombinant Fusion Proteins
Carbohydrates
Electrophoresis, Polyacrylamide Gel
HeLa Cells
Models, Biological
Glycophorin
The major sialoglycoprotein of the human erythrocyte membrane. It consists of at least two sialoglycopeptides and is composed of 60% carbohydrate including sialic acid and 40% protein. It is involved in a number of different biological activities including the binding of MN blood groups, influenza viruses, kidney bean phytohemagglutinin, and wheat germ agglutinin.
Cattle
Mutagenesis, Site-Directed
Fractals
Patterns (real or mathematical) which look similar at different scales, for example the network of airways in the lung which shows similar branching patterns at progressively higher magnifications. Natural fractals are self-similar across a finite range of scales while mathematical fractals are the same across an infinite range. Many natural, including biological, structures are fractal (or fractal-like). Fractals are related to "chaos" (see NONLINEAR DYNAMICS) in that chaotic processes can produce fractal structures in nature, and appropriate representations of chaotic processes usually reveal self-similarity over time.
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Integrin alpha Chains
The alpha subunits of integrin heterodimers (INTEGRINS), which mediate ligand specificity. There are approximately 18 different alpha chains, exhibiting great sequence diversity; several chains are also spliced into alternative isoforms. They possess a long extracellular portion (1200 amino acids) containing a MIDAS (metal ion-dependent adhesion site) motif, and seven 60-amino acid tandem repeats, the last 4 of which form EF HAND MOTIFS. The intracellular portion is short with the exception of INTEGRIN ALPHA4.
Oligosaccharides
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Mycobacteriophages
Ligaments, Articular
Vinculin
Actins
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Protein Processing, Post-Translational
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
Peptide Mapping
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
Transfection
Sequence Analysis, DNA
N-Acetylneuraminic Acid
Cytoskeleton
CHO Cells
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Genes
Sialic Acids
Chickens
Mass Spectrometry
Matrix Attachment Region Binding Proteins
Carbohydrate Sequence
Extracellular Matrix
Conjugation, Genetic
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Models, Molecular
Erythrocyte Membrane
SUMO-1 Protein
Larva
Immunologic Techniques
Collagen
Chondroitin Sulfate Proteoglycans
Nuclear Envelope
Sequence Analysis
Microscopy, Fluorescence
Carrier Proteins
Cell Adhesion Molecules
Freeze Fracturing
Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
Structure-Activity Relationship
Biomechanical Phenomena
Epithelial Attachment
Phenotype
Embryo, Nonmammalian
Macromolecular Substances
Nucleic Acid Conformation
DNA-Binding Proteins
DNA Primers
Chromatography, High Pressure Liquid
Salmonella typhimurium
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Cyanogen Bromide
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Lipid A
Saccharomyces cerevisiae
Drosophila melanogaster
Operon
Genetic Vectors
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Stress, Mechanical
Lactococcus lactis
Chick Embryo
Sialoglycoproteins
Blotting, Southern
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Cytoskeletal Proteins
Gene Expression Regulation, Developmental
Gene Expression
Rabbits
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Surface Properties
Nuclear Proteins
Glycolipids
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Repetitive Sequences, Nucleic Acid
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
DNA, Complementary
Streptomyces
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Muscle Development
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Saccharomyces cerevisiae Proteins
Transcription, Genetic
Metamorphosis, Biological
Microfilament Proteins
Green Fluorescent Proteins
Adhesins, Bacterial
Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.
Receptors, Cell Surface
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Cell Movement
COS Cells
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. (1/353)
Lysogenic bacteriophages are major vehicles for the transfer of genetic information between bacteria, including pathogenicity and/or virulence determinants. In the enteric pathogen Escherichia coli O157:H7, which causes hemorrhagic colitis and hemolytic-uremic syndrome, Shiga toxins 1 and 2 (Stx1 and Stx2) are phage encoded. The sequence and analysis of the Stx2 phage 933W is presented here. We find evidence that the toxin genes are part of a late-phage transcript, suggesting that toxin production may be coupled with, if not dependent upon, phage release during lytic growth. Another phage gene, stk, encodes a product resembling eukaryotic serine/threonine protein kinases. Based on its position in the sequence, Stk may be produced by the prophage in the lysogenic state, and, like the YpkA protein of Yersinia species, it may interfere with the signal transduction pathway of the mammalian host. Three novel tRNA genes present in the phage genome may serve to increase the availability of rare tRNA species associated with efficient expression of pathogenicity determinants: both the Shiga toxin and serine/threonine kinase genes contain rare isoleucine and arginine codons. 933W also has homology to lom, encoding a member of a family of outer membrane proteins associated with virulence by conferring the ability to survive in macrophages, and bor, implicated in serum resistance. (+info)Criss-crossed interactions between the enhancer and the att sites of phage Mu during DNA transposition. (2/353)
A bipartite enhancer sequence (composed of the O1 and O2 operator sites) is essential for assembly of the functional tetramer of phage Mu transposase (MuA) on supercoiled DNA substrates. A three-site interaction (LER) between the left (L) and right (R) ends of Mu (att sites) and the enhancer (E) precedes tetramer assembly. We have dissected the role of the enhancer in tetramer assembly by using two transposase proteins that have a common att site specificity, but are distinct in their enhancer specificity. The activity of these proteins on substrates containing hybrid enhancers reveals a 'criss-crossed' pattern of interaction between att and enhancer sites. The left operator, O1, of the enhancer interacts specifically with the transposase subunit at the R1 site (within the right att sequence) that is responsible for cleaving the left end of Mu. The right operator, O2, shows a preferential interaction with the transposase subunit at the L1 site (within the left att sequence) that is responsible for cleaving the right end of Mu. (+info)Site-specific recombination of bacteriophage P22 does not require integration host factor. (3/353)
Site-specific recombination by phages lambda and P22 is carried out by multiprotein-DNA complexes. Integration host factor (IHF) facilitates lambda site-specific recombination by inducing DNA bends necessary to form an active recombinogenic complex. Mutants lacking IHF are over 1,000-fold less proficient in supporting lambda site-specific recombination than wild-type cells. Although the attP region of P22 contains strong IHF binding sites, in vivo measurements of integration and excision frequencies showed that infecting P22 phages can perform site-specific recombination to its maximum efficiency in the absence of IHF. In addition, a plasmid integration assay showed that integrative recombination occurs equally well in wild-type and ihfA mutant cells. P22 integrative recombination is also efficient in Escherichia coli in the absence of functional IHF. These results suggest that nucleoprotein structures proficient for recombination can form in the absence of IHF or that another factor(s) can substitute for IHF in the formation of complexes. (+info)The genetic relationship between virulent and temperate Streptococcus thermophilus bacteriophages: whole genome comparison of cos-site phages Sfi19 and Sfi21. (4/353)
The virulent cos-site Streptococcus thermophilus bacteriophage Sfi19 has a 37,392-bp-long genome consisting of 44 open reading frames all encoded on the same DNA strand. The genome of the temperate cos-site S. thermophilus phage Sfi21 is 3.3 kb longer (40,740 bp, 53 orfs). Both genomes are very similarly organized and differed mainly by gene deletion and DNA rearrangement events in the lysogeny module; gene replacement, duplication, and deletion events in the DNA replication module, and numerous point mutations. The level of point mutations varied from <1% (lysis and DNA replication modules) to >15% (DNA packaging and head morphogenesis modules). A dotplot analysis showed nearly a straight line over the left 25 kb of their genomes. Over the right genome half, a more variable dotplot pattern was observed. The entire lysogeny module from Sfi21 comprising 12 genes was replaced by 7 orfs in Sfi19, six showed similarity with genes from temperate pac-site S. thermophilus phages. None of the genes implicated in the establishment of the lysogenic state (integrase, superinfection immunity, repressor) or remnants of it were conserved in Sfi19, while a Cro-like repressor was detected. Downstream of the highly conserved DNA replication module 11 and 13 orfs were found in Sfi19 and phiSfi21, respectively: Two orfs from Sfi21 were replaced by a different gene and a duplication of the phage origin of replication in Sfi19; a further orf was only found in Sfi21. All other orfs from this region, which included a second putative phage repressor, were closely related between both phages. Two noncoding regions of Sfi19 showed sequence similarity to pST1, a small cryptic plasmid of S. thermophilus. (+info)Comparative sequence analysis of the DNA packaging, head, and tail morphogenesis modules in the temperate cos-site Streptococcus thermophilus bacteriophage Sfi21. (5/353)
The temperate Streptococcus thermophilus bacteriophage Sfi21 possesses 15-nucleotide-long cohesive ends with a 3' overhang that reconstitutes a cos-site with twofold hyphenated rotational symmetry. Over the DNA packaging, head and tail morphogenesis modules, the Sfi21 sequence predicts a gene map that is strikingly similar to that of lambdoid coliphages in the absence of any sequence similarity. A nearly one to one gene correlation was found with the phage lambda genes Nu1 to H, except for gene B-to-E complex, where the Sfi21 map resembled that of coliphage HK97. The similarity between Sfi21 and HK97 was striking: both major head proteins showed an N-terminal coiled-coil structure, the mature major head proteins started at amino acid positions 105 and 104, respectively, and both major head genes were preceded by genes encoding a possible protease and portal protein. The purported Sfi21 protease is the first viral member of the ClpP protease family. The prediction of Sfi21 gene functions by reference to the gene map of intensively investigated coliphages was experimentally confirmed for the major head and tail gene. Phage Sfi21 shows nucleotide sequence similarity with Lactococcus phage BK5-T and a lactococcal prophage and amino acid sequence similarity with the Lactobacillus phage A2 and the Staphylococcus phage PVL. PVL is a missing link that connects the portal proteins from Sfi21 and HK97 with respect to sequence similarity. These observations and database searches, which demonstrate sequence similarity between proteins of phage from gram-positive bacteria, proteobacteria, and Archaea, constrain models of phage evolution. (+info)Alternative mechanism of cholera toxin acquisition by Vibrio cholerae: generalized transduction of CTXPhi by bacteriophage CP-T1. (6/353)
Horizontal transfer of genes encoding virulence factors has played a central role in the evolution of many pathogenic bacteria. The unexpected discovery that the genes encoding cholera toxin (ctxAB), the main cause of the profuse secretory diarrhea characteristic of cholera, are encoded on a novel filamentous phage named CTXPhi, has resulted in a renewed interest in the potential mechanisms of transfer of virulence genes among Vibrio cholerae. We describe here an alternative mechanism of cholera toxin gene transfer into nontoxigenic V. cholerae isolates, including strains that lack both the CTXPhi receptor, the toxin coregulated pilus (TCP), and attRS, the chromosomal attachment site for CTXPhi integration. A temperature-sensitive mutant of the V. cholerae generalized transducing bacteriophage CP-T1 (CP-T1ts) was used to transfer a genetically marked derivative of the CTX prophage into four nontoxigenic V. cholerae strains, including two V. cholerae vaccine strains. We demonstrate that CTXPhi transduced by CP-T1ts can replicate and integrate into these nontoxigenic V. cholerae strains with high efficiency. In fact, CP-T1ts transduces the CTX prophage preferentially when compared with other chromosomal markers. These results reveal a potential mechanism by which CTXPhi(+) V. cholerae strains that lack the TCP receptor may have arisen. Finally, these findings indicate an additional pathway for reversion of live-attenuated V. cholerae vaccine strains. (+info)The IntI1 integron integrase preferentially binds single-stranded DNA of the attC site. (7/353)
IntI1 integrase is a member of the prokaryotic DNA integrase superfamily. It is responsible for mobility of antibiotic resistance cassettes found in integrons. IntI1 protein, as well as IntI1-COOH, a truncated form containing its carboxy-terminal domain, has been purified. Electrophoretic mobility shift assays were carried out to study the ability of IntI1 to bind the integrase primary target sites attI and aadA1 attC. When using double-stranded DNA as a substrate, we observed IntI1 binding to attI but not to attC. IntI1-COOH did not bind either attI or attC, indicating that the N-terminal domain of IntI1 was required for binding to double-stranded attI. On the other hand, when we used single-stranded (ss) DNA substrates, IntI1 bound strongly and specifically to ss attC DNA. Binding was strand specific, since only the bottom DNA strand was bound. Protein IntI1-COOH bound ss attC as well as did the complete integrase, indicating that the ability of the protein to bind ss aadA1 attC was contained in the region between amino acids 109 and 337 of IntI1. Binding to ss attI DNA by the integrase, but not by IntI1-COOH, was also observed and was specific for the attI bottom strand, indicating similar capabilities of IntI1 for binding attI DNA in either double-stranded or ss conformation. Footprinting analysis showed that IntI1 protected at least 40 bases of aadA1 attC against DNase I attack. The protected sequence contained two of the four previously proposed IntI1 DNA binding sites, including the crossover site. Preferential ssDNA binding can be a significant activity of IntI1 integrase, which suggests the utilization of extruded cruciforms in the reaction mechanisms leading to cassette excision and integration. (+info)TPW22, a lactococcal temperate phage with a site-specific integrase closely related to Streptococcus thermophilus phage integrases. (8/353)
The temperate phage TPW22, induced from Lactococcus lactis subsp. cremoris W22, and the evolutionarily interesting integrase of this phage were characterized. Phage TPW22 was propagated lytically on L. lactis subsp. cremoris 3107, which could also be lysogenized by site-specific integration. The attachment site (attP), 5'-TAAGGCGACGGTCG-3', of phage TPW22 was present on a 7.5-kb EcoRI fragment, a 3.4-kb EcoRI-HindIII fragment of which was sequenced. Sequence information revealed the presence of an integrase gene (int). The deduced amino acid sequence showed 42 and 28% identity with integrases of streptococcal and lactococcal phages, respectively. The identities with these integrase-encoding genes were 52 and 45%, respectively, at the nucleotide level. This could indicate horizontal gene transfer. A stable integration vector containing attP and int was constructed, and integration in L. lactis subsp. cremoris MG1363 was obtained. The existence of an exchangeable lactococcal phage integration module was suggested. The proposed module covers the phage attachment site, the integrase gene, and surrounding factor-independent terminator structures. The phages phiLC3, TP901-1, and TPW22 all have different versions of this module. Phylogenetically, the TPW22 Int links the phiLC3 lactococcal integrase with known Streptococcus thermophilus integrases. (+info)
Team:LMU-Munich/Jump-or-die - 2010.igem.org
E. coli plasmid vector pLDR11 - incomplete.
Pagina informativa
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68374 w[1118]; P{y[+t7.7] w[+mC]=R82D11-p65.AD}attP40/CyO; P{y[+t7.7] w[+mC]=R41C07-GAL4.DBD}attP2/TM6B, Tb[1]
68334 w[1118]; P{y[+t7.7] w[+mC]=VT026773-p65.AD}attP40; P{y[+t7.7] w[+mC]=R72B05-GAL4.DBD}attP2
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intact or incomplete PROPHAGE
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Site-specific deletion and rearrangement of integron insert genes catalyzed by the integron DNA integrase. | Journal of...
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Peri-implant mucositis
Microbiological testing was shown to improve the prognostic features compared to recording bleeding on probing alone as this ... Increased levels of bleeding on probing was present at 67% of sites where there is peri-implant mucositis as it is indicative ... Increased probing depths over time is linked to loss of attachment and a reduction in the supporting alveolar bone levels. When ... This is especially true with respect to their surrounding tissues and biological attachment. The diagnosis of peri-implant ...
Sphaerotilus natans
An adhesive basal element at one end of the filament can aid attachment to solid surfaces. The sheath offers some protection ... Individual mature cells swarm out of the protective tube to colonize new sites. Each motile mature cell has an intertwined ... Van Veen, WL; Mulder, EG; Deinema, MH (1978). "The Sphaerotilus-Leptothrix group of bacteria". Microbiological Reviews. 42 (2 ...
Mixotricha paradoxa
Cysts never form, so transmission from one host to another is always based on direct contact between the sites they occupy. ... "Movement symbiosis". www.microbiological-garden.net. Retrieved 27 May 2019. Rosenberg, Eugene; Gophna, Uri (30 August 2011). ... attachment and cyst formation". Eur. J. Protistol. 43 (4): 281-94. doi:10.1016/j.ejop.2007.06.004. PMID 17764914. Brugerolle G ... "Movement symbiosis2". www.microbiological-garden.net. Retrieved 27 May 2019. König, Helmut (2006). Intestinal Microorganisms of ...
Bacteriophage T12
The bacterial attachment site (attB) has a 96 base pair sequence homologous to the phage attachment site and is located at the ... Microbiological Research. 156 (1): 35-40. doi:10.1078/0944-5013-00087. PMID 11372651. S. Atsumi; J. W. Little (2006). "Role of ... The phage integrase gene (int) and the phage attachment site (attp) are located just upstream of the speA gene in the phage ... And finally, in 1997, McShan and Ferretti published that they had found the second attachment site (attR) for T12, while also ...
Immunofluorescence
The direct attachment of the messenger to the antibody reduces the number of steps in the procedure, saving time and reducing ... Antigenic material must be fixed firmly on the site of its natural localization inside the cell. Intact antibodies can also be ... is a technique used for light microscopy with a fluorescence microscope and is used primarily on microbiological samples. This ...
Corneal ulcer
the site of the ulcer is studied and marked in case sheet.the edge of the ulcer is studied.whether satellite lesion is present ... Microbiological culture tests may be necessary to isolate the causative organisms for some cases. Other tests that may be ... They are believed to be caused by a defect in the basement membrane and a lack of hemidesmosomal attachments. They are ... https://www.aao.org/eye-health/diseases/corneal-ulcer https://www.merckmanuals.com/home/eye-disorders/corneal-disorders/corneal ...
Asthma-related microbes
It may cause injury to the respiratory epithelial cell after its attachment. The injury of host epithelial cells caused by M. ... Microbiological Reviews. 58: 686-699. PMC 372987. PMID 7854252. Pasternak, Y. "Mycoplasma pneumoniae". Microbe Wiki. Retrieved ... a process that could potentially alter receptor recognition sites and affect cytokine induction and expression. As stated by ...
Endomembrane system
"Microbiological Reviews. 55 (4): 543-60. PMC 372837. PMID 1779926.. *^ Voelker DR (July 2005). "Bridging gaps in phospholipid ... By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ... Other proteins on the plasma membrane allow attachment to the cytoskeleton and extracellular matrix; a function that maintains ... Levine T, Loewen C (August 2006). "Inter-organelle membrane contact sites: through a glass, darkly". Current Opinion in Cell ...
Vesicle (biology and chemistry)
"Microbiological Reviews. 58: 94-144. doi:10.1128/mmbr.58.1.94-144.1994. PMC 372955. PMID 8177173.. ... By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ... Membrane proteins serving as receptors are sometimes tagged for downregulation by the attachment of ubiquitin. After arriving ... "Microbiological Reviews. 58 (1): 94-144. doi:10.1128/mmbr.58.1.94-144.1994. PMC 372955. PMID 8177173.. .mw-parser-output cite. ...
Marburg virus
By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ... The marburg virus life cycle begins with virion attachment to specific cell-surface receptors, followed by fusion of the virion ... "Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition". Retrieved 2011-10-16.. ... The mean evolutionary rate of the whole genome was 3.3 × 10−4 substitutions/site/year (credibility interval 2.0-4.8).The ...
Scaling and root planing
Arestin, a popular site specific brand of the antibiotic minocycline, is claimed to enable regaining of at least 1 mm of ... Gingival attachment begins to loosen further as the bacterial plaque continues to invade the space created by the swelling it ... partial-mouth disinfection in the treatment of periodontal infections: short-term clinical and microbiological observations". ... Site specific antibiotics may also be placed in the periodontal pocket following scaling and root planing in order to provide ...
Marine life
"The Earth Life Web, Growth and Development in Lichens". earthlife.net. Silliman B. R. & S. Y. Newell (2003). "Fungal farming in ... CABI, 2008 Hyde, K.D.; E.B.J. Jones (1989). "Spore attachment in marine fungi". Botanica Marina. 32 (3): 205-218. doi:10.1515/ ... Cavalier-Smith T (December 1993). "Kingdom protozoa and its 18 phyla". Microbiological Reviews. 57 (4): 953-94. doi:10.1128/ ... They form the base of the primary production that drives the ocean food web, and account for half of the current global primary ...
Chronic periodontitis
... pockets are sites where the attachment has been gradually destroyed by collagen-destroying enzymes, known as collagenases) ... or specific microbiological profile, can benefit more from this adjunctive therapy." Chemical antimicrobials may be used by the ... With type 2 diabetes patients being shown to have 3.8 times more bone loss and 2.8 times more clinical attachment loss than non ... The cumulative effects of alveolar bone loss, attachment loss and pocket formation is more apparent with an increase in age. ...
Lactic acid bacteria
This attachment is mediated by the phage's receptor binding protein (RBP), which recognizes and binds to a receptor on the ... See the NCBI webpage on Lactobacillales Data extracted from "NCBI Taxonomy Browser". National Center for Biotechnology ... 2004). Lactic Acid Bacteria: Microbiological and Functional Aspects (3rd ed.). New York: Marcel Dekker, Inc. ISBN 978-0-8247- ... The first contact between an infecting phage and its bacterial host is the attachment of the phage to the host cell. ...
Trichomonas vaginalis
The site currently contains all of the T. vaginalis sequence project data, several EST libraries, and tools for data mining and ... The axostyle may be used for attachment to surfaces and may also cause the tissue damage seen in trichomoniasis infections. The ... Petrin D, Delgaty K, Bhatt R, Garber G (April 1998). "Clinical and microbiological aspects of Trichomonas vaginalis". Clin. ... TrichDB: the Trichomonas vaginalis genome resource NIH site on trichomoniasis. Taxonomy eMedicine article on trichomoniasis. ...
Biosensor
This side of the glass sensor chip can be modified in a number of ways, to allow easy attachment of molecules of interest. ... The integration of the fluorophore must be done in a site where it is sensitive to the binding of the analyte without ... vitamin B12 and pantothenic acid as an alternative to microbiological assay, determination of drug residues in food, such as ... The residues that form the binding site for a given antigen, are selected among the hypervariable residues. It is possible to ...
Virus quantification
There are kits and serums commercially available for this assay (e.g. The Binding Site Inc.). TEM is a specialized type of ... This assay is based on a microbiological method conducted in petri dishes or multi-well plates. Specifically, a confluent ... In this variation, serum antibodies to the influenza virus will interfere with the virus attachment to red blood cells. ...
Aggressive periodontitis
GAP brings about attachment loss involving more than 30% of sites on teeth;[1] effectively being at least three permanent teeth ... "Use and interpretation of microbiological assays in periodontal diseases". Oral Microbiology and Immunology. 1 (1): 73-81. ... LAP is localised to first molar or incisor interproximal attachment loss, whereas GAP is the interproximal attachment loss ... Episodic nature of attachment loss: Two main tissue responses have been found in GAP cases:[31] *Tissue may have severe acute ...
Filoviridae
"Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition". Retrieved 2011-10-16. US National Institutes of ... The filovirus life cycle begins with virion attachment to specific cell-surface receptors, followed by fusion of the virion ... The family Filoviridae (/ˌfaɪloʊˈvɪrɪdiː/), a member of the order Mononegavirales, is the taxonomic home of several related ... 4 nucleotide substitutions/site/year. The most recent common ancestor of sequenced filovirus variants was estimated to be 1971 ...
Clostridium novyi
The cells of the microvascular system become spherical and the attachments to neighbouring cells are reduced to thin strings. ... from infections associated with the injection of drugs: experiences of a microbiological investigation team". Journal of ... "Autopsy findings in an outbreak of severe systemic illness in heroin users following injection site inflammation: an effect of ...
Marine protists
This refers to the two whip-like attachments (flagella) used for forward movement. Most dinoflagellates are protected with red- ... "Amoebae: Protists Which Move and Feed Using Pseudopodia". Tree of Life web project. "The Amoebae". The University of Edinburgh ... Cavalier-Smith T (December 1993). "Kingdom protozoa and its 18 phyla". Microbiological Reviews. 57 (4): 953-94. doi:10.1128/ ... "Welcome to the Phaeocystis antarctica genome sequencing project homepage". DiTullio, G. R.; Grebmeier, J. M.; Arrigo, K. R.; ...
Glutamine synthetase
Each active site creates a 'tunnel' which is the site of three distinct substrate binding sites: nucleotide, ammonium ion, and ... Adenylylation is a post-translational modification involving the covalent attachment of AMP to a protein side chain. Each ... Merrick MJ, Edwards RA (December 1995). "Nitrogen control in bacteria". Microbiological Reviews. 59 (4): 604-22. doi:10.1128/MR ... GDP, AMP, ADP bind to the ATP site. L-serine, L-alanine, and glycine bind to the site for L-glutamate in unadenylated GS. The ...
Neomycin
By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ... Next is the attachment of the D-ribose via ribosylation of neamine, using 5-phosphoribosyl-1-diphosphate (PRPP) as the ribosyl ... "Comparative study of responses to neomycins B and C by microbiological and gas-liquid chromatographic assay methods". Applied ... The association constant for neomycin with A-site RNA has been found to be in the 109 M−1 range.[25] However, more than 50 ...
Listeria
"Microbial attachment to food and food contact surfaces". In: Advances in Food and Nutrition Research, Vol. 43. ed. Taylor, S. L ... "CDC - Listeria - Home". cdc.gov/listeria. Retrieved 15 June 2019. Temple, M. E.; Nahata, M. C. (May 2000). "Treatment of ... Salo S.; Laine A.; Alanko T.; Sjoberg A. M.; Wirtanen G. (2000). "Validation of the microbiological methods Hygicult dipsilde, ... Kalmokoff M. L.; Austin J. W.; Wan X. D.; Sanders G.; Banerjee S.; Farber J. M. (2001). "Adsorption, attachment and biofilm ...
Biofilm
... which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some ... An ecologic shift away from balanced populations within the dental biofilm is driven by certain (cariogenic) microbiological ... Initial attachment Irreversible attachment Maturation I Maturation II Dispersion Dispersal of cells from the biofilm colony is ... Each hamus is a long tube with three hook attachments that are used to attach to each other or to a surface, enabling a ...
Dental health diets for dogs
Clarke, D.E. (2001). "Clinical and Microbiological Effects of Oral Zinc Ascorbate Gel in Cats". Journal of Veterinary Dentistry ... are able to bind directly to the enamel as well as the mineralized sections of the teeth where it takes up the binding sites ... and loss of attachment in beagle dogs". Journal of Periodontal Research. 18 (4): 452-458. doi:10.1111/j.1600-0765.1983.tb00382. ...
Bacteria
"Microbiological Reviews. 58 (1): 94-144. doi:10.1128/MMBR.58.1.94-144.1994. PMC 372955. PMID 8177173.. ... By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ... Fimbriae are believed to be involved in attachment to solid surfaces or to other cells, and are essential for the virulence of ... "Microbiological Reviews. 57 (2): 434-50. doi:10.1128/MMBR.57.2.434-450.1993. PMC 372918. PMID 8336674.. ...
Influenza
"Microbiological Reviews. 56 (1): 152-79. PMC 372859. PMID 1579108.. *^ Zambon MC (November 1999). "Epidemiology and ... The different sites of infection (shown in red) of seasonal H1N1 versus avian H5N1. This influences their lethality and ability ... van Riel D, Munster VJ, de Wit E, Rimmelzwaan GF, Fouchier RA, Osterhaus AD, Kuiken T (April 2006). "H5N1 Virus Attachment to ... "Microbiological Reviews. 56 (1): 152-79. PMC 372859. PMID 1579108.. *. Steinhauer DA, Skehel JJ (2002). "Genetics of influenza ...
Cell damage
Web. 21 Mar 2013. *^ Festjens, Nele; Vanden Berghe, Tom; Vandenabeele, Peter (2006-09-01). "Necrosis, a well-orchestrated form ... loosening of intercellular attachments. *mitochondrial changes. *dilation of the endoplasmic reticulum. Fatty change[edit]. The ... By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ... Oxidative DNA damages usually involve only one of the DNA strands at any damaged site, but about 1-2% of damages involve both ...
DNA
... the Double Helix Game From the official Nobel Prize web site ... It may act as a recognition factor to regulate the attachment ... "Microbiological Reviews. 57 (2): 434-50. doi:10.1128/MMBR.57.2.434-450.1993. PMC 372918. PMID 8336674.. ... "Microbiological Reviews. 58 (3): 563-602. doi:10.1128/MMBR.58.3.563-602.1994. PMC 372978. PMID 7968924.. ... By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ...
Virus
Microbiological Reviews. 1 June 1993;57(2):434-50. PMID 8336674.. *^ Barrangou R, Fremaux C, Deveau H, et al.. CRISPR provides ... Harmful Algal Blooms: Red Tide: Home [Retrieved 2014-12-19].. *^ Hall A. J., Jepson P. D., Goodman S. J., Harkonen T.. Phocine ... Attachment to the receptor can induce the viral envelope protein to undergo changes that results in the fusion of viral and ... By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ...
Marburg virus disease
By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ... "Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition". Retrieved 2011-10-16.. ... The marburgvirus life cycle begins with virion attachment to specific cell-surface receptors, followed by fusion of the virion ... Symptoms include bloody stools, ecchymoses, blood leakage from venipuncture sites, mucosal & visceral hemorrhaging, and ...
Bakteria, kamusi elezo huru
LSU Law Center's Medical and Public Health Law Site, Historic Public Health Articles. Iliwekwa mnamo 2006-11-23. ... Beachey E (1981). "Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface ... "Microbiological Reviews 58 (1): 94-144. PMC 372955 . PMID 8177173 . http://mmbr.asm.org/cgi/pmidlookup?view=long&pmid=8177173. ... Bacterial Chemotaxis Interactive Simulator - A web-app that uses several simple algorithms to simulate bacterial chemotaxis. ...
Neisseria meningitidis
By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ... The gold standard of diagnosis is microbiological isolation of N. meningitidis by growth from a sterile body fluid, which could ... fimbriae mediate attachment of the bacterium to the epithelial cells of the nasopharynx.[14][15] It infects the cell by ... Anyone who frequently ate, slept or stayed at the patient's home during the 7 days before the onset of symptom, or those who ...
Antibiotic use in livestock
... store animal waste in lagoons on site.[15] Manure is also trucked off site, stored in containers, or held in holding ponds. ... "Evaluating the Safety of Antimicrobial New Animal Drugs with Regard to Their Microbiological Effects on Bacteria of Human ... https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/662189/UK_AMR_3rd_annual_report ... By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ...
Solar still
By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ... A separate plastic bag hangs from attachment points on the outer bag. Seawater is poured into the inner bag from an opening in ... This process leaves behind impurities, such as salts and heavy metals, and eliminates microbiological organisms. The end result ...
Metabolism
The flow of protons makes the stalk subunit rotate, causing the active site of the synthase domain to change shape and ... Proteins are made from amino acids that have been activated by attachment to a transfer RNA molecule through an ester bond. ... Häse CC, Finkelstein RA (December 1993). "Bacterial extracellular zinc-containing metalloproteases". Microbiological Reviews. ... Microbiological Reviews. 60 (4): 609-40. doi:10.1128/MMBR.60.4.609-640.1996. PMC 239458. PMID 8987358. Barea JM, Pozo MJ, Azcón ...
Periodontal disease
... pockets are sites where the attachment has been gradually destroyed by collagen-destroying enzymes, known as collagenases) ... The correlation of selected microbiological parameters with disease severity in Sri Lankan tea workers". Journal of Clinical ... of attachment loss Moderate: 3-4 mm (0.12-0.16 in) of attachment loss Severe: ≥ 5 mm (0.20 in) of attachment loss The "extent" ... Sites are defined as the positions at which probing measurements are taken around each tooth and, generally, six probing sites ...
List of ASTM International standards (F)
ASTM Standards site List of A ASTM standards List of B ASTM standards List of C ASTM standards List of D ASTM standards List of ... Test Methods for Microbiological Monitoring of Water Used for Processing Electron and Microelectronic Devices by Direct ... Specification for Performance of Hasps and Other Attachment Devices for Padlocks or Seals F2156-17 Test Method for Measuring ... Test Method for Determining Energy Consumption of Vacuum Cleaners F2757-20 Guide for Home Laundering Care and Maintenance of ...
Staphylococcus aureus bacteriophages mediating the simultaneous lysogenic conversion of beta-lysin, staphylokinase and...
Attachment Sites, Microbiological * Bacterial Toxins* * Blood Proteins * Cross Infection / microbiology * DNA, Recombinant ... The entA and sak determinants were closely linked in the phage DNA adjacent to the phage attachment site (attP) in each case ... restriction endonuclease site mapping and hybridization analysis, and compared with the mechanism of beta-lysin and ...
Structural features of single-stranded integron cassette attC sites and their role in strand selection
The lack of sequence conservation among attC sites led us to hypothesize that sequence-independe … ... were occurring through unconventional site-specific recombination reactions involving only the bottom strand of attC sites. ... Attachment Sites, Microbiological* * Base Sequence * DNA, Bacterial / chemistry* * DNA, Bacterial / genetics * DNA, Single- ... Structural features of single-stranded integron cassette attC sites and their role in strand selection PLoS Genet. 2009 Sep;5(9 ...
Periodontal Status in Smokers and Nonsmokers: A Clinical, Microbiological, and Histopathological Study
... sites showing attachment loss ,4 mm,sites showing attachment loss between 4-7 mm,sites showing attachment loss ,7 mm. ... Microbiological Examination. BANA test is done to assess the microbiological status.. One site with the deepest probing depth ... sites showing ,4 mm of probing depth,sites showing 4-7 mm of probing depth,sites showing ,7 mm of probing depth. ... Sampling methods vary widely, and, together with undoubted differences from site to site within the mouth, such variations may ...
Robust HPV-18 production in organotypic cultures of primary human keratinocytes. - NextBio article
Clinical, Microbiological and Biochemical Effects of the Antimicrobial Photodynamic Therapy - Full Text View - ClinicalTrials...
Improvement in Clinical Attachment Level [ Time Frame: 6 months ]. Secondary Outcome Measures : *Improvement in Periodontal ... The subject has at least 4 sites with PD of ≥5 mm + bleeding in at least two quadrants of the mouth. ... Clinical, Microbiological and Biochemical Effects of the Antimicrobial Photodynamic Therapy. The safety and scientific validity ... Clinical, Microbiological and Biochemical Effects of the Antimicrobial Photodynamic Therapy. Study Start Date :. November 2011 ...
Diagnosis and Management of Tickborne Rickettsial Diseases: Rocky Mountain Spotted Fever and Other Spotted Fever Group...
Attachment sites of four tick species (Acari: Ixodidae) parasitizing humans in Georgia and South Carolina. J Med Entomol 1999; ... Biosafety in microbiological and biomedical laboratories (BMBL). 5th ed. Washington, DC: US Department of Health and Human ... Sites where ticks commonly attach to humans include, but are not limited to, the scalp, abdomen, axillae, and groin, as well as ... CDC is not responsible for the content of pages found at these sites. URL addresses listed in MMWR were current as of the date ...
The front line of host defense - Immunobiology - NCBI Bookshelf
Microorganisms that cause pathology in humans and animals enter the body at different sites and produce disease by a variety of ... a normal flora of nonpathogenic bacteria that compete with pathogenic microorganisms for nutrients and for attachment sites on ... Surface epithelia provide mechanical, chemical, and microbiological barriers to infection. In addition to these defenses, most ... This specific attachment allows the pathogen to infect the epithelial cell, or to damage it so that the epithelium can be ...
Non Surgical Therapy of Periodontitis in Diabetes Patients: the Adjunctive Use of Probiotics - Tabular View - ClinicalTrials.gov
Full-mouth clinical measurements of PPD and clinical attachment level (CAL) will be taken at 6 sites per tooth, excluding third ... Microbiological samples will be taken. The patient smoking history will be recorded and participants will be categorized as: ... Follow up visits after 3 and 6 months Follow up visits will have the objective to monitor the clinical and microbiological ... The aim of this study is to evaluate the clinical and microbiological performance of a probiotic formulation (Sunstar GUM ...
Membrane process for spring water treatment in the Tula Valley: assessment of physicochemical and microbiological parameters in...
... because soil organic matter provides hydrophobic binding sites for virus attachment (Schijven & Hassanizadeh 2000). In this ... This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy. ... Membrane process for spring water treatment in the Tula Valley: assessment of physicochemical and microbiological parameters in ... assessment of physicochemical and microbiological parameters in a non-conventional water source. Water Supply 1 April 2015; 15 ...
ZFIN Publication: Bedell et al., 2012
Attachment Sites, Microbiological/genetics. *Base Sequence. *Chromosomes/genetics. *DNA Breaks. *DNA, Single-Stranded/genetics ... Home page banner reprinted from Hearing Research, 341, Monroe, J.D. et al., Hearing sensitivity differs between zebrafish lines ... including the introduction of a custom-designed EcoRV site and a modified loxP (mloxP) sequence into somatic tissue in vivo. We ...
Coupling of transparent exopolymer particle dynamics and microbiological processes during an ocean acidification experiment in...
In addition, TEP are an important structural component since they provide attachment sites for microbes on a nanometer to ... Coupling of transparent exopolymer particle dynamics and microbiological processes during an ocean acidification experiment in ... Coupling of transparent exopolymer particle dynamics and microbiological processes during an ocean acidification experiment in ... TEP may be altered due to short term responses to acidification.We observed that the amount of TEP as well as microbiological ...
Microbiological diagnosis of biofilm-related infections | Enfermedades Infecciosas y Microbiología Clínica (English Edition)
... the hosts proteins cover the internal and external surfaces of the device and serve as an attachment site for certain ... Microbiological diagnosis of biofilm-related infections Diagnóstico microbiológico de las infecciones relacionadas con la ... The microbiological diagnosis is based on the similarity in isolations obtained from the culture from the insertion point or ... Microbiological diagnosis is generally reserved for cases which are refractory to treatment. The clinical sample should be ...
Binding of Haemophilus du… - University of Gothenburg, Sweden
Attachment Sites, Microbiological, physiology, Binding Sites, Carbohydrate Sequence, Chancroid, microbiology, Chromatography, ... Our results suggest that the 58.5-kDa GroEL HSP of H. ducreyi is responsible for the attachment of this bacterium to the ... To elucidate the role of the surface-located 58.5-kDa GroEL heat shock protein (HSP) of H. ducreyi in attachment, we ... To identify carbohydrate receptors that mediate the attachment of this pathogen to host cells, we investigated the binding of ...
Severe Periodontal Disease Associated with Long-Term Treatment with Intravenous Immunoglobulin
No significant alterations in genetic or microbiological features were observed. The present case suggests that periodontal ... View at: Publisher Site , Google Scholar*Y.-M. Wu, J. Yan, L.-L. Chen, W.-L. Sun, and Z.-Y. Gu, "Infection frequency of Epstein ... Microbiological and Genetic Analysis. To better explore mechanisms that could explain the severe periodontal status, ... Clinical and radiographic examinations demonstrated a severe and generalized periodontal disease, with clinical attachment loss ...
Peri-implant mucositis - Wikipedia
Microbiological testing was shown to improve the prognostic features compared to recording bleeding on probing alone as this ... Increased levels of bleeding on probing was present at 67% of sites where there is peri-implant mucositis as it is indicative ... Increased probing depths over time is linked to loss of attachment and a reduction in the supporting alveolar bone levels. When ... This is especially true with respect to their surrounding tissues and biological attachment. The diagnosis of peri-implant ...
Pesquisa | Portal Regional da BVS
Prevalence of periodontally active sites was low in the study population. Microbiological studies: Black pigmented anaerobic ... and clinical attachment loss (CAL), were examined at mesial-buccal and distal-lingual sites of each tooth. Number of missing ... clinical attachment level (CAL), and bleeding on probing (BOP). A total 4,765 periodontal sites were evaluated, 125 of which ... Subgingival microbiological profile of periodontitis patients in Dominican Republic. Collins, James R; Chinea, Sofía; Cuello, ...
Comparison of the role of attachment, aggregation and internalisation of microorganisms in UVC and UVA (solar) disinfection on...
Article Comparison of the role of attachment, aggregation and internalisation of microorganisms in UVC and UVA (solar) ... physicochemical and microbiological characteristics of water samples taken seasonally from selected sampling sites on seven ... Comparison of the role of attachment, aggregation and .... Comparison of the role of attachment, aggregation and ... No comments were found for Comparison of the role of attachment, aggregation and internalisation of microorganisms in UVC and ...
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- University of Illinois at Urbana-Champaign
Microbiological Attachment Sites 2012 Altered regulation of escherichia coli biotin biosynthesis in bira superrepressor mutant ... Biotin and lipoic acid: Synthesis, attachment, and regulation. Cronan, J. E., Jan 1 2014, In : EcoSal Plus. 6, 1, p. 1-39 39 p. ... Overlapping repressor binding sites result in additive regulation of Escherichia coli FadH by FadR and ArcA. Feng, Y. & Cronan ...
The Journal of Contemporary Dental Practice
We use cookies on this site to enhance your user experience.. By clicking any link on this page you are giving your consent for ... Clinical and microbiological effects of sustained release of chlorhexidine in periodontal pockets. J clinical periodontol, 1986 ... Clinical parameters like gingival index, probing depth, attachment loss, and gingival shrinkage were evaluated along with ... Microbiological characteristics of subgingival microbiota in adult periodontitis, localized juvenile periodontitis and rapidly ...
Frontiers | Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young...
The same occurs for A. viscosus (22% of sites) and A. actinomycetemcomitans (6% of sites; Table 3). ... Microbiological Analysis. Total DNA Extraction. Total DNA was isolated from the interdental brushes using the QIAcube® HT ... 3 mm or clinical attachment loss (CAL) > 3 mm; and (iv) the subjects were judged to be free of gingivitis or periodontitis. ... For all subjects, the same four interdental sites (15-16, 25-26, 35-36, and 45-46) were assessed (total 100 sites). The ...
tetracycline - Trip Database
Since tetracycline is an important antibiotic for treating Ct, zoonotic infections at mutual sites of infection indicate the ... Plaque score, gingival score, pocket probing depth, and clinical attachment level were evaluated before treatment and at 1 ... A clinico microbiological study. (Abstract). Comparison of the efficacy of subgingival irrigation with 2% povidone-iodine and ... We validated TetRP by confirming the translation start sites of protein-coding genes in accordance with the 2006 version of ...
topic:"Dental Plaque Index" found 96 records • Arctic Health
Gains in attachment (0.9 mm and 1.1 mm) were observed in minocycline-treated sites, with baseline probing depth , or =5 mm and ... Microbiological sampling using DNA probes was done at baseline; at week 2; and at months 1, 3, 6, 9, 12, and 15. Both treatment ... versus 1.2 mm in the control sites. Sites with a baseline probing depth , or =7 mm and bleeding index ,2 showed an average of ... sites treated with minocycline ointment always produced statistically significantly greater reductions than sites which ...
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This site complies with the HONcode standard for trustworthy health information: Verify here. ... Health Protection Agency (HPA). Guidelines for Assessing the Microbiological Safety of Ready-to-Eat Foods Placed on the Market ... Available from: https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/363146/ ... Guidelines_for_assessing_the_microbiological_safety_of_ready-to-eat_foods_on_the_market.pdf ...
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OPUS at UTS: ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastrointestinal LS174T epithelial cells - Open...
Attachment Sites, Microbiological. en_US. dc.subject.mesh. ATP Binding Cassette Transporter, Subfamily B. en_US. ... ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastrointestinal LS174T epithelial cells. Crowe, A Bebawy, M ... Irrespective of attachment differences between the bacterial species, the increase in P-gp protein expression decreased ... Irrespective of attachment differences between the bacterial species, the increase in P-gp protein expression decreased ...
Sequence analysis and characterization of phi O1205, a temperate bacteriophage infecting Streptococcus thermophilus CNRZ1205. ...
Citations may include links to full-text content from PubMed Central and publisher web sites. ... The phage attachment site attP, the bacterial attachment site attB, and the two phage/chromosome junctions attL and attR were ... Attachment Sites, Microbiological. *Base Sequence. *DNA, Viral/genetics. *Genome, Viral*. *Molecular Sequence Data ...
Pathogenicity islands of virulent bacteria: structure, function and impact on microbial evolution | Meta
Sphaerotilus natans - Wikipedia
An adhesive basal element at one end of the filament can aid attachment to solid surfaces. The sheath offers some protection ... Individual mature cells swarm out of the protective tube to colonize new sites. Each motile mature cell has an intertwined ... Van Veen, WL; Mulder, EG; Deinema, MH (1978). "The Sphaerotilus-Leptothrix group of bacteria". Microbiological Reviews. 42 (2 ...
Metabolism - Wikipedia
The flow of protons makes the stalk subunit rotate, causing the active site of the synthase domain to change shape and ... Proteins are made from amino acids that have been activated by attachment to a transfer RNA molecule through an ester bond. ... Häse CC, Finkelstein RA (December 1993). "Bacterial extracellular zinc-containing metalloproteases". Microbiological Reviews. ... Microbiological Reviews. 60 (4): 609-40. doi:10.1128/MMBR.60.4.609-640.1996. PMC 239458. PMID 8987358. Barea JM, Pozo MJ, Azcón ...
PeriodontitisParametersPhysicochemical and microbiologicalSubgingivalGingival indexBinding SitesBaselineInitial periodontalGingivalisPhage attachment siteRadiographicMethodsOutcomesChronicCompare the clinicalInfectionGenesRecombinationSequenceEscherichiaAttPPlaqueOrganismAquacultureStudyClinical attachment lossGeneticAntimicrobialInfectious diseasesMediateEfficacyVitroPocketsGenomeSignificantlyDiagnosisLevelTreatmentSingle-strandedPathogenTestAttBVesiclesDepthMeasurementsResultsIntegrationDifferent attachment2017
Periodontitis13
- The goal of this study is to determine if patients with chronic periodontitis when undergoing with scaling and root planing (SRP) plus photodynamic therapy in comparison with SRP alone result in improved clinical, microbiological and biochemical outcomes. (clinicaltrials.gov)
- Microbiological characteristics of subgingival microbiota in adult periodontitis, localized juvenile periodontitis and rapidly progressive periodontitis subjects. (thejcdp.com)
- Comparison of the efficacy of subgingival irrigation with 2% povidone-iodine and tetracycline HCl in subjects with chronic moderate periodontitis: A clinico microbiological study. (tripdatabase.com)
- We hypothesized that levels of microbiological mediators and / or inflammatory markers of chronic periodontitis would be intermediate in those exposed to environmental tobacco smoke compared to active and non-smokers. (tobaccopreventioncessation.com)
- when used in conjunction with initial periodontal treatment in adult periodontitis patients, achieves significantly better clinical and microbiological results than initial periodontal treatment alone. (rug.nl)
- The clinical parameters of probing depth, clinical attachment level, bleeding on probing, plaque accumulation and suppuration were measured in 60 Caucasian adults who were divided into two groups: 30 healthy individuals (control) and 30 with chronic periodontitis (ChP). (cdc.gov)
- This way, this project is aimed to evaluate the pattern of microbiological colonization and the inflammatory response pattern associated with it, comparing parents with generalized aggressive periodontitis and their children and periodontally healthy parents and their children. (clinicaltrials.gov)
- AbstractObjective: To evaluate the clinical and microbiological effects of systemicazithromycin as an adjunct to scaling and root planing (SRP) in the treatment ofPorphyromonas gingivalis-associated chronic periodontitis. (medtheses.com)
- scaling and root Conclusions: Within the limitations of this study, the adjunctive use of systemic azithromycin in the treatment of P. gingivalis periodontitis demonstrated significantclinical and microbiological benefits when compared with SRP plus placebo. (medtheses.com)
- aetiology of periodontitis is multi-factor- ial, but it is an infection and bacterial pills were provided by Pfizer, Alcobendas, microbial spectrum with in vitro activity attachment loss (Winkel et al. (medtheses.com)
- In this study, evaluation of the effect of mechanical debridement and adjunctive metronidazol plus spiramycin therapy on the clinical and microbiological parameters of Early-Onset Periodontitis patients was aimed. (selcuk.edu.tr)
- Periodontitis is a prevalent disease of men characterized by loss of connective tissue attachment and bone around the teeth, in conjunction with the formation of periodontal pockets due to apical migration of the junctional epithelium. (dentalnews.com)
- Consequently, elevated levels of these putative pathogens may be useful indicators of both active periodontitis and increased risk of gingival attachment loss. (dentalnews.com)
Parameters10
- A case-control study was done to assess the influence of smoking on clinical, microbiological, and histopathological parameters. (hindawi.com)
- Aim: To evaluate the effect of subgingival placement of doxycycline in the form of a resorbable membrane on the clinical and microbiological parameters in the treatment of inflammatory periodontal disease. (thejcdp.com)
- Clinical parameters like gingival index, probing depth, attachment loss, and gingival shrinkage were evaluated along with microbiological evaluation as well for a period of 10 weeks. (thejcdp.com)
- The full-mouth periodontal clinical parameters, clinical attachment level (CAL), probing depth (PD), gingival index (GI), and plaque index (PI), were monitored at baseline, 3, 6, and 9 months after periodontal treatment with full mouth disinfection (FMD) protocol. (bvsalud.org)
- For clinical and microbiological parameters, two-factor repeated-measures ANOVA was used. (bvsalud.org)
- Key secondary variables were additional periodontal, microbiological and psychological parameters. (eurekaselect.com)
- Clinical and microbiological parameters were measured 6 weeks before drawing blood to determine levels of HbA1C. (unipi.it)
- Periodontal parameters were measured at baseline (TO), year 2 (T2), year 3 (T3) and included: probing depth (PD), attachment level (AL), sulcus bleeding index (SBI), and plaque index (PI). (unipi.it)
- Test site comprised of SRP followed by intra-pocket placement of Aloe vera gel, which was compared with the control site in which only SRP was done, and clinical parameters were compared between the two sites at one month and three months from baseline. (jisponline.com)
- Clinical parameters like gingival index (GI), plaque index (PI) and probing pocket depth (PPD), clinical attachment level (CAL) were evaluated at baseline and 3 months follow-up. (scribd.com)
Physicochemical and microbiological1
- In this study, physicochemical and microbiological characteristics of water samples taken seasonally from selected sampling sites on seven main canals in Kafr El-Sheikh Governorate were. (environmental-expert.com)
Subgingival4
- Subgingival plaque samples were collected from 6 sites per subject and analyzed for 38 bacterial species using the Checkerboard DNA-DNA Hybridization. (cdc.gov)
- We hypothesized that adjunctive antibiotic therapy significantly reduces (Δ50%) the occurrence of further attachment loss compared to subgingival debridement alone. (eurekaselect.com)
- During this period, samples of gingival crevicular fluid (GCF) and subgingival biofilm from periodontal pockets/sites from all subject (parents and children) will be collected. (clinicaltrials.gov)
- Sites evaluated clinically with Probing Pocket Depths, Gingival Index, Plaque Index and Clinical Attachment Gain and assessed microbiologically with bacteriologic culture method for Aa, Pg, Pi and with microscopic method for the presence of spirochetes at stained subgingival plaque samples. (selcuk.edu.tr)
Gingival index2
- It was observed that the gingival index score, periodontal probing depth, and gingival shrinkage showed low levels at the doxycycline-treated sites as compared to the placebo group. (thejcdp.com)
- Each of the sites was assessed for the plaque index, gingival index, and probing pocket depth at baseline and 21 days and for microbiological analysis at baseline, 1 week and 21 days. (jisponline.com)
Binding Sites3
- Identification of cis regulatory features in the embryonic zebrafish genome through large-scale profiling of H3K4me1 and H3K4me3 binding sites. (umassmed.edu)
- We attribute this unusual phenotype to differences in the distribution of int protein binding sites among different attachment sites (Ross and Landy 1982, 1983). (princeton.edu)
- We suggest that int protein molecules bound to one of two recombining DNAs interact with empty sites or with bound proteins on the other, and that the mutant protein acts efficiently only if the distribution of protein binding sites within the two attachment sites is that of the attP-attB pair. (princeton.edu)
Baseline8
- Standard clinical variables (including probing depth and attachment level) were evaluated at baseline and at months 1, 3, 6, 9, 12, and 15. (arctichealth.org)
- Clinical measurements and microbiological assessments were taken at baseline and 3 months after completion of initial periodontal therapy with additional placebo or antibiotic treatment. (rug.nl)
- In contrast, the changes in the proportions of sites with a probing pocket depth greater than or equal to5 mm in the Pg-neg placebo and Pg-neg test subgroup were similar, from 43% at baseline to 18% after treatment versus 40% to 12%, respectively. (rug.nl)
- Bleeding on probing, probing depth and clinical attachment level were recorded at baseline, 6 and 12 months postoperatively. (unito.it)
- PD and AL of constant sites in the diabetic group at T3 were significantly higher than baseline. (unipi.it)
- There was a significant increase in Prevotella intermedia at T3 as compared with baseline for deepest sites in the diabetic group. (unipi.it)
- Clinical [plaque and bleedingindexes, probing pocket depth (PPD), clinical attachment level (CAL)] andmicrobiological data (four-sites pooled samples, processed by culture) were collectedat baseline, and 1, 3 and 6 months, post-therapy. (medtheses.com)
- Counts units per millilitre of the original sam- sites selected at baseline), clinical para- bacterial species were also calculated. (medtheses.com)
Initial periodontal1
- A systematic review on the use of residual probing depth, bleeding on probing and furcation status following initial periodontal therapy to predict further attachment and tooth loss. (panlodowegoogrodu.pl)
Gingivalis2
- 4 subgroups were created on the basis of the initial microbiological status for P. gingivalis positive (Pg-pos) and negative patients (Pg-neg) in the P- and the T-groups. (rug.nl)
- The adjunctive use of tetracycline consistently resulted in a lower percentages of sites with detectable levels of A. actinomycetemcomitans, P. intermedia and P. gingivalis, while no differences were detected between treatments in the prevalence of T. denticola and B. forsythus. (unito.it)
Phage attachment site2
- The entA and sak determinants were closely linked in the phage DNA adjacent to the phage attachment site (attP) in each case and furthermore, the sak determinant of phage phi 13 was also located near its attP. (nih.gov)
- The phage attachment site attP, the bacterial attachment site attB, and the two phage/chromosome junctions attL and attR were identified and found to contain a 40 bp common core sequence. (nih.gov)
Radiographic3
- Smokers have been associated with deeper pockets and greater attachment loss, more pronounced radiographic evidence of furcation involvement, and increased alveolar bone loss. (hindawi.com)
- Significant positive correlations were observed between the level of GCF SLIT3 and clinical attachment level and radiographic bone loss (p (bvsalud.org)
- Traditionally, periodontal diseases have been diagnosed by radiographic and clinical exams, including the assessment of plaque with a plaque index (PI), gingival inflammation with the bleeding on probing index (BOP), probing pocket depth (PPD) and clinical attachment loss (CAL). (dentalnews.com)
Methods3
- METHODS: A total of 44 bilateral pockets 4-5 mm deep and bleeding on probing were selected in 11 non-smokers patients, 22 treated by scaling and root planing plus tetracycline fibres (test sites) and 22 with scaling and root planing alone (control sites) in a split-mouth design. (unito.it)
- Methods for collecting and transporting microbiological specimens and for recovering detectable antigen are also described. (google.com)
- Two methods of larval probiont delivery were tested - attachment to Artemia, and in-water delivery. (openthesis.org)
Outcomes1
- Conclusions Obesity did not affect the clinical and microbiological outcomes of non-surgical periodontal therapy. (bvsalud.org)
Chronic1
- This study is a prospective, parallel, comparative, clinical, hematological and microbiological study performed in patients with generalized chronic periodontal disease separated in two groups, with 10 patients with diabetes mellitus insulin-dependent (DM) and 10 patients non-diabetics (NDM) attended by 3 months. (unicamp.br)
Compare the clinical2
- The present study aimed to compare the clinical and microbiological effects of non-surgical periodontal therapy with the full mouth disinfection (FMD) protocol on obese and non-obese individuals at 9 months post-therapy. (bvsalud.org)
- Abstract: The aim of this study was to compare the clinical, hematological and microbiological responses of periodontal treatment for both groups after full-mouth scaling and root planning. (unicamp.br)
Infection7
- Thus in most cases these defenses, which we will examine in more detail in subsequent sections, prevent a site of infection from being established. (nih.gov)
- Infectious disease occurs when a microorganism succeeds in evading or overwhelming innate host defenses to establish a local site of infection and replication that allows its further transmission. (nih.gov)
- In this hypothesis, the external fixator construct appears to be vital to the prevention of pin site complications since excessive movement at the fixator pin-bone interface leads to pin site irritation and infection. (springer.com)
- Symptoms of staph infections include reddish, swollen, itchy, and tender area at the site of infection. (emedicinehealth.com)
- Most staph infections that are visible usually have a reddish, swollen, itchy, and/or tender area at the site of infection. (emedicinehealth.com)
- Sites of infection can be small like a pimple on someone's face or even in the nose, a boil on an extremity, or large like a carbuncle. (emedicinehealth.com)
- There was no supervisor infection in the site breathing. (minestudio.com.my)
Genes1
- These strains contain combinations of deletions or mutations in the bacterial attachment site for Φ80 integration and/or deletions in the genes required for phage absorption to the host outer membrane. (illinois.edu)
Recombination10
- We recently showed that cassette integration and deletion in integron platforms were occurring through unconventional site-specific recombination reactions involving only the bottom strand of attC sites. (nih.gov)
- Here, we systematically determined the contribution of three structural elements common to all known single-stranded attC site recombination substrates (the EHBs, the unpaired central spacer (UCS), and the variable terminal structure (VTS)) to strand choice and recombination. (nih.gov)
- We found that the structure of the central spacer is essential to achieve high level recombination of the bottom strand, suggesting a dual role for this structure in active site exclusion and for hindering the reverse reaction after the first strand exchange. (nih.gov)
- Cultures of V. cholerae strains with deletions in the dif recombination site are known to contain a subpopulation of cells that display a filamentous morphology3. (nih.gov)
- These observations strongly suggest that φBT1 encodes a site-specific recombination system that has a different specificity from that in φC31 and therefore integrates into a different attB site in the S. coelicolor genome. (asm.org)
- Five of the remaining eight enzymes were active on extra-chromosomal substrates thereby demonstrating that the ability to mediate extra-chromosomal recombination is no guide to ability to mediate site-specific recombination on integrated DNA. (ox.ac.uk)
- All the integrases that were active on integrated DNA also promoted DNA integration reactions that were not mediated through conservative site-specific recombination or damaged the recombination sites but the extent of these aberrant reactions varied over at least an order of magnitude. (ox.ac.uk)
- This mutant promotes efficient recombination between the phage and bacterial attachment sites, but, unlike wild type, does not promote efficient recombination of any other pair of attachment sites tested in most conditions. (princeton.edu)
- IN particular, recombination between two phage or two prophage attachment sites is poor relative to the wild type frequency. (princeton.edu)
- Similar discrimination among attachment site pairs by wild type int protein may also modulate recombination frequencies. (princeton.edu)
Sequence5
- The lack of sequence conservation among attC sites led us to hypothesize that sequence-independent structural recognition determinants must exist within attC sites. (nih.gov)
- With this updated TALEN system, we successfully used single-stranded DNA oligonucleotides to precisely modify sequences at predefined locations in the zebrafish genome through homology-directed repair, including the introduction of a custom-designed EcoRV site and a modified loxP ( mloxP ) sequence into somatic tissue in vivo . (zfin.org)
- The commonly used plant constitutive expression vector pBI121 was modified by insertion of two directly orientated lox sites each at one end of the selectable marker gene NPTII and by replacing the GUS gene with a sequence composed of multiple cloning sites (MCS). (bvsalud.org)
- Moreover, to facilitate screening of selectable marker-removed gene and the composite sequence is flanked by lox sites. (bvsalud.org)
- Furthermore, no significant similarity could be detected between the φC31 attP site and any φBT1 sequence. (asm.org)
Escherichia3
- Attachment of Escherichia coli to human urinary tract epithelial cells. (meta.org)
- Furthermore, a vector, pSET152 containing the φC31 attP-int locus, introduced by conjugation from Escherichia coli can integrate into secondary or pseudo- attB sites in both S. coelicolor and Streptomyces lividans ( 8 ). (asm.org)
- Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle. (naver.com)
AttP1
- We demonstrate that φBT1 does indeed integrate into a different attB site in S. coelicolor , and we have constructed novel integrating vectors derived from the φBT1 attP-int locus. (asm.org)
Plaque5
- Clinical periodontal measurements, comprising plaque index, probing depth, clinical attachment level and bleeding on probing, were recorded at four sites per tooth. (tobaccopreventioncessation.com)
- Except for the plaque, there was a significantly larger change in the bleeding, probing pocket depth (PPD) and clinical attachment level (CAL) in the T-group as compared to the P-group after therapy. (rug.nl)
- All individuals (parents and children) will be clinically assessed for plaque and bleeding index, periodontal probing depth, clinical attachment level and gingival recession. (clinicaltrials.gov)
- Full-mouth clinical examinations were carried out and Visible Plaque Index (VPI), Gingival Bleeding Index (GBI), Probing Pocket Depth (PPD), and clinical attachment level (CAL) were assessed in 6 sites per tooth. (ufrgs.br)
- Traditional clinical measurements (probing pocket depth, bleeding on probing, clinical attachment loss, plaque index, radiographs) used for periodontal diagnosis are often of limited usefulness in that they are indicators of previous periodontal disease rather than present disease activity. (dentalnews.com)
Organism1
- For these reasons and as many workers would like to use two compatible integrating vectors in the same organism, we have investigated the integration site of the Streptomyces phage φBT1, a homoimmune relative of φC31. (asm.org)
Aquaculture1
- Manipulation or control of the microbiological aquaculture environment has been identified as an important focus area for future hatchery development. (openthesis.org)
Study3
- The aim of this study is to evaluate the clinical and microbiological performance of a probiotic formulation (Sunstar GUM Periobalance) as adjunctive to non-surgical periodontal therapy in patients with diabetes. (clinicaltrials.gov)
- Request single or consolidated IRB review for a multi-site study. (harvard.edu)
- AIM: The purpose of this study was to evaluate the clinical and microbiological efficacy of local delivery of tetracycline in persistent periodontal lesions after topical therapy. (unito.it)
Clinical attachment loss3
- depth of >3 and ≤5 mm and clinical attachment loss of 3-4 mm in relation to 16, 36, and 46. (tripdatabase.com)
- CAL: Clinical attachment loss. (aapd.org)
- Bacterial samples were collected with paper points from periodontal sites showing the deepest periodontal pocket depth associated with the highest clinical attachment loss. (bvsalud.org)
Genetic1
- No significant alterations in genetic or microbiological features were observed. (hindawi.com)
Antimicrobial1
- To reduce the number of candidate probiotics to be tested in vivo, various in vitro experiments need to be conducted, each screening for a particular mode of action - antagonism towards pathogen through production of antimicrobial compounds, growth and attachment to fish intestinal mucus, and the production of other beneficial compounds such as vitamins, fatty-acids and digestive enzymes. (openthesis.org)
Infectious diseases1
- It has as its aim to respond to the challenges currently posed by everything associated with infectious diseases, from a clinical, microbiological and public health perspective. (elsevier.es)
Mediate1
- To identify carbohydrate receptors that mediate the attachment of this pathogen to host cells, we investigated the binding of 35S-methionine-labeled H. ducreyi strains to a panel of defined glycosphingolipids that were separated on thin layer chromatography plates. (gu.se)
Efficacy1
- The primary objective efficacy variable was the percentage of sites with a loss of clinical attachment ≥ 1.3 mm over the 24-month follow-up period. (eurekaselect.com)
Vitro1
- Different in vitro (Pajukanta 1993) specific microbiological profiles, it has and in vivo (Herrera et al. (medtheses.com)
Pockets2
- The null hypothesis is that the adjunctive use of this probiotic formulation would not have any additional benefit over scaling and root planning in the number of residual pockets or in the microbiological impact of the treatment. (clinicaltrials.gov)
- The pathogens could be eliminated from 3 periodontal pockets by debridement alone and from 12 sites by tetracycline at 12 months. (unito.it)
Genome4
- We found that acquisition and chromosomal integration of the TLC-Knφ1 genome restored a perfect dif site and normal morphology to V. cholerae wild-type and mutant strains with dif(-) filamentation phenotypes. (nih.gov)
- Southern blots of DNA from an S. coelicolor J1929 φBT1 lysogen (strain J1929 contains Δ pglY conferring sensitivity to φC31 and φBT1 [ 3 ]) probed with DNA encoding the φC31 attB site indicated that φC31 attB was intact, suggesting that φBT1 was integrated elsewhere in the genome (data not shown). (asm.org)
- A nuclear matrix attachment region (MAR) spans oriP and the adjacent region of the EBV genome containing the EBV-expressed RNAs. (ox.ac.uk)
- CONCLUSIONS: We conclude that the Bxb1 and φC31 integrases are the reagents of choice for genome engineering in vertebrate cells and that DNA damage repair is a major limitation upon the utility of this class of site-specific recombinase. (ox.ac.uk)
Significantly2
- Mean probing pocket depth and clinical attachment level significantly increased in the high antibody group. (bvsalud.org)
- When differences were detected, sites treated with minocycline ointment always produced statistically significantly greater reductions than sites which received the vehicle control. (arctichealth.org)
Diagnosis1
- The microbiological tests have the potential to support the diagnosis of various forms of periodontal disease, to serve as indicators of disease initiation and progression and to determine which periodontal sites are at higher risk for active destruction. (dentalnews.com)
Level4
- The reduction of bleeding on probing scores was 86.4% and 40.9%, the decrease of probing depth was 2.25 mm and 1.19 mm and the gain of clinical attachment level was 2.04 mm and 0.64 mm for test and control groups, respectively, over the 12-month period. (unito.it)
- Attachment in adolescence: A move to the level of emotion regulation. (levitra2020.site)
- Prognosis periodontal treatment Once all of the steps of the treatment plan have been carried out, the microbiological test is then repeated to certify that the mouth has regained a healthy ecosystem, hence showing a clear prevalence of saprophytes and a level of percentage of pathogens that the immune system is able to easily manage. (panlodowegoogrodu.pl)
- If the follow experiences directions, acne medication with accutane swelling of the level, antibiotics or voorhoofd or has lantana promiseit, contact the cjd about, as these are teeth of an microbiological broad-spectrum. (minestudio.com.my)
Treatment1
- Standardised pin site protocols which encompass an understanding of external fixator biomechanics and meticulous surgical technique during pin and wire insertion, postoperative pin site care and pin removal could limit the incidence of major infections and treatment failures. (springer.com)
Single-stranded1
- Conjugation was used to deliver the attC sites in single-stranded form. (nih.gov)
Pathogen1
- Candidate probiont Pseudoalteromonas AP5 reduced the attachment ability of Vibrio alginolyticus when added before the pathogen and partially out-competed the pathogen for attachment sites when added second. (openthesis.org)
Test4
- or =5 mm, a mean reduction in probing depth of 1.9 mm was seen in the test sites, versus 1.2 mm in the control sites. (arctichealth.org)
- To test this further and to identify the φBT1 attB site, we performed vectorette PCR (Sigma-Genosys) extending outwards from the φBT1 DNA into the host DNA in an S. coelicolor φBT1 lysogen. (asm.org)
- Mean values were obtained and Wilcoxon and paired sample t tests were used to compare the test and control sites. (ufrgs.br)
- Test sites received scaling and root planing along with green tea catechin strips and control sites received scaling and root planning alone. (jisponline.com)
AttB4
- Despite their wide use and clear advantages, it has been reported that integration of these vectors into the φC31 attB site can cause detrimental effects on antibiotic production in some strains ( 2 ). (asm.org)
- The reported reductions in antibiotic synthesis could be caused by insertional mutagenesis into SCO3798 or by integration into one of the pseudo- attB sites or some other factor. (asm.org)
- Another potential problem with integrating vectors could be the absence of an efficiently recognized attB site in some streptomycete strains. (asm.org)
- Indeed, Saccharopolyspora erythraea appears to lack a φC31 attB site (P. Leadlay, personal communication). (asm.org)
Vesicles1
- Doxycycline should soon be used in doxycycline pets humans regimens or in ill vesicles where it can result in microbiological stroke staining of compositions and have fetal prescriptions on female doctor infinity. (gastro-service-info.de)
Depth3
- The difference in reduction of PPD between Pg-pos and Pg-neg patients was particularly evident with respect to the changes in % of sites with a probing pocket depth greater than or equal to5 mm. (rug.nl)
- Two sites in each patient were selected for microbial samples: a mesio-facial aspect of the maxillary right first molar (defined as constant site, CS) and a site with the greatest probing depth (defined as deepest site, DS). (unipi.it)
- Fourteen patients with two sites in the contralateral quadrants with probing pocket depth of 5−8mm were selected. (jisponline.com)
Measurements1
- Thus, the relation between autotrophic N2-fixation and heterotrophic activity (uptake of radiolabeld Leucine) was determined and compared with TEP concentration measurements to elucidate how production and fate of TEP may be altered due to short term responses to acidification.We observed that the amount of TEP as well as microbiological activities were sensitive to changes in pCO2. (awi.de)
Results2
- Our results suggest that the 58.5-kDa GroEL HSP of H. ducreyi is responsible for the attachment of this bacterium to the majority of the tested glycosphingolipids, and thus represents a potential bacterial adhesin. (gu.se)
- RESULTS: The changes over the 10 years with respect to frequency of diseased sites indicated an increased frequency in continuous smokers versus decreased frequencies in former smokers and non-smokers. (arctichealth.org)
Integration3
- Pais are often associated with tRNA loci, which may represent target sites for the chromosomal integration of these elements. (meta.org)
- Furthermore, lysogeny of a dif(-) non-toxigenic V. cholerae with TLC-Knφ1 promoted its subsequent toxigenic conversion through integration of CTXφ into the restored dif site. (nih.gov)
- Analysis of the integrity of the yecE gene, a possible stx phage integration site in EHEC O157, in the consecutive stx(2)-positive and stx-negative isolates demonstrated that yecE was occupied in stx(2)-positive but intact in stx-negative strains. (unboundmedicine.com)
Different attachment1
- Here we demonstrate that φBT1 integrates into a different attachment site than φC31. (asm.org)
20172
- We updated the design of this site on December 18, 2017. (clinicaltrials.gov)
- Nejsum, Lene N. 2017-08-01 00:00:00 To target bacterial pathogens that invade and proliferate inside host cells, it is necessary to design intervention strategies directed against bacterial attachment, cellular invasion and intracellular proliferation. (deepdyve.com)