OrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation Science
ATP PhosphoribosyltransferaseHypoxanthine PhosphoribosyltransferaseAdenine PhosphoribosyltransferasePentosyltransferasesFructose-BisphosphataseAdenosine MonophosphateEscherichia coli ProteinsBinding SitesEscherichia coliKineticsAllosteric RegulationMycobacterium tuberculosisPentosesTuberculosisTuberculosis, PulmonaryAntitubercular AgentsHistidineAllosteric SiteMycobacteriumCorynebacterium glutamicumBiosensing TechniquesCorynebacteriumLigandsBiotechnologyHigh-Throughput Screening AssaysTissue EngineeringPyruvate Dehydrogenase (Lipoamide)Dihydrolipoamide DehydrogenasePyruvate Dehydrogenase ComplexPyruvate Dehydrogenase Complex Deficiency DiseaseAcetyl-CoA CarboxylaseAcetate-CoA LigaseCarbon-Nitrogen LigasesAmino AcidsKetoglutaric AcidsEncyclopedias as TopicCitric Acid CycleCitrate (si)-SynthaseGlutamate DehydrogenaseKetoglutarate Dehydrogenase Complex
ATP Phosphoribosyltransferase
An enzyme that catalyzes the first step of the pathway for histidine biosynthesis in Salmonella typhimurium. ATP reacts reversibly with 5-phosphoribosyl-1-pyrophosphate to yield N-1-(5'-phosphoribosyl)-ATP and pyrophosphate. EC 2.4.2.17.
Hypoxanthine Phosphoribosyltransferase
An enzyme that catalyzes the conversion of 5-phosphoribosyl-1-pyrophosphate and hypoxanthine, guanine, or 6-mercaptopurine to the corresponding 5'-mononucleotides and pyrophosphate. The enzyme is important in purine biosynthesis as well as central nervous system functions. Complete lack of enzyme activity is associated with the LESCH-NYHAN SYNDROME, while partial deficiency results in overproduction of uric acid. EC 2.4.2.8.
Adenine Phosphoribosyltransferase
An enzyme catalyzing the formation of AMP from adenine and phosphoribosylpyrophosphate. It can act as a salvage enzyme for recycling of adenine into nucleic acids. EC 2.4.2.7.
Pentosyltransferases
Enzymes of the transferase class that catalyze the transfer of a pentose group from one compound to another.
Fructose-Bisphosphatase
An enzyme that catalyzes the conversion of D-fructose 1,6-bisphosphate and water to D-fructose 6-phosphate and orthophosphate. EC 3.1.3.11.
Adenosine Monophosphate
Adenine nucleotide containing one phosphate group esterified to the sugar moiety in the 2'-, 3'-, or 5'-position.
Escherichia coli Proteins
Proteins obtained from ESCHERICHIA COLI.
Binding Sites
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Kinetics
The rate dynamics in chemical or physical systems.
Allosteric Regulation
The modification of the reactivity of ENZYMES by the binding of effectors to sites (ALLOSTERIC SITES) on the enzymes other than the substrate BINDING SITES.
Mycobacterium tuberculosis
A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.
Pentoses
A class of carbohydrates that contains five carbon atoms.
Tuberculosis
Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.
Tuberculosis, Pulmonary
MYCOBACTERIUM infections of the lung.
Antitubercular Agents
Drugs used in the treatment of tuberculosis. They are divided into two main classes: "first-line" agents, those with the greatest efficacy and acceptable degrees of toxicity used successfully in the great majority of cases; and "second-line" drugs used in drug-resistant cases or those in which some other patient-related condition has compromised the effectiveness of primary therapy.
Histidine
An essential amino acid that is required for the production of HISTAMINE.
Allosteric Site
A site on an enzyme which upon binding of a modulator, causes the enzyme to undergo a conformational change that may alter its catalytic or binding properties.
Mycobacterium
A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts.
Corynebacterium glutamicum
A species of gram-positive, asporogenous, non-pathogenic, soil bacteria that produces GLUTAMIC ACID.
Biosensing Techniques
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
Corynebacterium
A genus of asporogenous bacteria that is widely distributed in nature. Its organisms appear as straight to slightly curved rods and are known to be human and animal parasites and pathogens.
Ligands
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
Biotechnology
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
High-Throughput Screening Assays
Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays.
Tissue Engineering
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
Pyruvate Dehydrogenase (Lipoamide)
The E1 component of the multienzyme PYRUVATE DEHYDROGENASE COMPLEX. It is composed of 2 alpha subunits (pyruvate dehydrogenase E1 alpha subunit) and 2 beta subunits (pyruvate dehydrogenase E1 beta subunit).
Dihydrolipoamide Dehydrogenase
A flavoprotein containing oxidoreductase that catalyzes the reduction of lipoamide by NADH to yield dihydrolipoamide and NAD+. The enzyme is a component of several MULTIENZYME COMPLEXES.
Pyruvate Dehydrogenase Complex
A multienzyme complex responsible for the formation of ACETYL COENZYME A from pyruvate. The enzyme components are PYRUVATE DEHYDROGENASE (LIPOAMIDE); dihydrolipoamide acetyltransferase; and LIPOAMIDE DEHYDROGENASE. Pyruvate dehydrogenase complex is subject to three types of control: inhibited by acetyl-CoA and NADH; influenced by the energy state of the cell; and inhibited when a specific serine residue in the pyruvate decarboxylase is phosphorylated by ATP. PYRUVATE DEHYDROGENASE (LIPOAMIDE)-PHOSPHATASE catalyzes reactivation of the complex. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed)
Pyruvate Dehydrogenase Complex Deficiency Disease
An inherited metabolic disorder caused by deficient enzyme activity in the PYRUVATE DEHYDROGENASE COMPLEX, resulting in deficiency of acetyl CoA and reduced synthesis of acetylcholine. Two clinical forms are recognized: neonatal and juvenile. The neonatal form is a relatively common cause of lactic acidosis in the first weeks of life and may also feature an erythematous rash. The juvenile form presents with lactic acidosis, alopecia, intermittent ATAXIA; SEIZURES; and an erythematous rash. (From J Inherit Metab Dis 1996;19(4):452-62) Autosomal recessive and X-linked forms are caused by mutations in the genes for the three different enzyme components of this multisubunit pyruvate dehydrogenase complex. One of the mutations at Xp22.2-p22.1 in the gene for the E1 alpha component of the complex leads to LEIGH DISEASE.
Acetyl-CoA Carboxylase
A carboxylating enzyme that catalyzes the conversion of ATP, acetyl-CoA, and HCO3- to ADP, orthophosphate, and malonyl-CoA. It is a biotinyl-protein that also catalyzes transcarboxylation. The plant enzyme also carboxylates propanoyl-CoA and butanoyl-CoA (From Enzyme Nomenclature, 1992) EC 6.4.1.2.
Acetate-CoA Ligase
An enzyme that catalyzes the formation of CoA derivatives from ATP, acetate, and CoA to form AMP, pyrophosphate, and acetyl CoA. It acts also on propionates and acrylates. EC 6.2.1.1.
Carbon-Nitrogen Ligases
Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3.
Amino Acids
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Ketoglutaric Acids
A family of compounds containing an oxo group with the general structure of 1,5-pentanedioic acid. (From Lehninger, Principles of Biochemistry, 1982, p442)
Encyclopedias as Topic
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Citric Acid Cycle
A series of oxidative reactions in the breakdown of acetyl units derived from GLUCOSE; FATTY ACIDS; or AMINO ACIDS by means of tricarboxylic acid intermediates. The end products are CARBON DIOXIDE, water, and energy in the form of phosphate bonds.
Citrate (si)-Synthase
Enzyme that catalyzes the first step of the tricarboxylic acid cycle (CITRIC ACID CYCLE). It catalyzes the reaction of oxaloacetate and acetyl CoA to form citrate and coenzyme A. This enzyme was formerly listed as EC 4.1.3.7.
Glutamate Dehydrogenase
An enzyme that catalyzes the conversion of L-glutamate and water to 2-oxoglutarate and NH3 in the presence of NAD+. (From Enzyme Nomenclature, 1992) EC 1.4.1.2.
Ketoglutarate Dehydrogenase Complex

An aminoacyl-tRNA synthetase paralog with a catalytic role in histidine biosynthesis. (1/30)

In addition to their essential catalytic role in protein biosynthesis, aminoacyl-tRNA synthetases participate in numerous other functions, including regulation of gene expression and amino acid biosynthesis via transamidation pathways. Herein, we describe a class of aminoacyl-tRNA synthetase-like (HisZ) proteins based on the catalytic core of the contemporary class II histidyl-tRNA synthetase whose members lack aminoacylation activity but are instead essential components of the first enzyme in histidine biosynthesis ATP phosphoribosyltransferase (HisG). Prediction of the function of HisZ in Lactococcus lactis was assisted by comparative genomics, a technique that revealed a link between the presence or the absence of HisZ and a systematic variation in the length of the HisG polypeptide. HisZ is required for histidine prototrophy, and three other lines of evidence support the direct involvement of HisZ in the transferase function. (i) Genetic experiments demonstrate that complementation of an in-frame deletion of HisG from Escherichia coli (which does not possess HisZ) requires both HisG and HisZ from L. lactis. (ii) Coelution of HisG and HisZ during affinity chromatography provides evidence of direct physical interaction. (iii) Both HisG and HisZ are required for catalysis of the ATP phosphoribosyltransferase reaction. This observation of a common protein domain linking amino acid biosynthesis and protein synthesis implies an early connection between the biosynthesis of amino acids and proteins.  (+info)

Specific binding of the first enzyme for histidine biosynthesis to the DNA of histidine operon. (2/30)

Studies were done to examine direct binding of the first enzyme of the histidine biosynthetic pathway (phosphoribosyltransferase) to 32P-labeled phi80dhis DNA and competition of this binding by unlabeled homologous DNA and by various preparations of unlabeled heterologous DNA, including that from a defective phi80 bacteriophage carrying the histidine operon with a deletion of part of its operator region. Our findings show that phosphoribosyltransferase binds specifically to site in or near the regulatory region of the histidine operon. The stability of the complex formed by interaction of the enzyme with the DNA was markedly decreased by the substrates of the enzyme and was slightly increased by the allosteric inhibitor, histidine. These findings are consistent with previous data that indicate that phosphoribosyltransferase plays a role in regulating expression of the histidine operon.  (+info)

Molecular dissection of the role of histidine in nickel hyperaccumulation in Thlaspi goesingense (Halacsy). (3/30)

To understand the role of free histidine (His) in Ni hyperaccumulation in Thlaspi goesingense, we investigated the regulation of His biosynthesis at both the molecular and biochemical levels. Three T. goesingense cDNAs encoding the following His biosynthetic enzymes, ATP phosphoribosyltransferase (THG1, GenBank accession no. AF003347), imidazoleglycerol phosphate dehydratase (THB1, GenBank accession no. AF023140), and histidinol dehydrogenase (THD1, GenBank accession no. AF023141) were isolated by functional complementation of Escherichia coli His auxotrophs. Northern analysis of THG1, THD1, and THB1 gene expression revealed that each gene is expressed in both roots and shoots, but at the concentrations and dosage times of Ni treatment used in this study, these genes failed to show any regulation by Ni. We were also unable to observe any increases in the concentration of free His in root, shoot, or xylem sap of T. goesingense in response to Ni exposure. X-ray absorption spectroscopy of root and shoot tissue from T. goesingense and the non-accumulator species Thlaspi arvense revealed no major differences in the coordination of Ni by His in these tissues. We therefore conclude that the Ni hyperaccumulation phenotype in T. goesingense is not determined by the overproduction of His in response to Ni.  (+info)

Molecular cloning and characterization of ATP-phosphoribosyl transferase from Arabidopsis, a key enzyme in the histidine biosynthetic pathway. (4/30)

We have characterized two isoforms of ATP-phosphoribosyl transferase (ATP-PRT) from Arabidopsis (AtATP-PRT1 [accession no. AB025251] and AtATP-PRT2), catalyzing the first step of the pathway of hisidine (His) biosynthesis. The primary structures deduced from AtATP-PRT1 and AtATP-PRT2 cDNAs share an overall amino acid identity of 74.6% and contain N-terminal chloroplast transit peptide sequences. DNA-blot analyses indicated that the ATP-PRTs in Arabidopsis are encoded by two separate genes with a closely similar gene structural organization. Both gene transcripts were detected throughout development, and protein-blot analysis revealed predominant accumulation of the AtATP-PRT proteins in Arabidopsis leaves. The His auxotrophy of a his1 mutant of Saccharomyces cerevisiae was suppressed by the transformation with AtATP-PRT1 and AtATP-PRT2 cDNAs, indicating that both isoforms are functionally active ATP-PRT enzymes. The K(m) values for ATP and phosphoribosyl pyrophosphate of the recombinant AtATP-PRT proteins were comparable to those of the native ATP-PRTs from higher plants and bacteria. It was demonstrated that the recombinant AtATP-PRTs were inhibited by L-His (50% inhibition of initial activity = 40-320 microM), suggesting that His biosynthesis was regulated in plants through feedback inhibition by L-His.  (+info)

Evidence against a covalent intermediate in the adenosine triphosphate phosphoribosyltransferase reaction of histidine biosynthesis. (5/30)

14C-Labeled 5-phospho-alpha-D-ribose-1-diphosphate (PRibPP) was synthesized and its interaction with adenosine triphosphate phosphoribosyltransferase was examined by gel filtration in a search for a form of this substrate covalently bound to the enzyme. Wide variation in solvent conditions gave little labeling of the enzyme. Heavy labeling was found only in the presence of the second substrate, ATP, and this was shown to arise from tightly but noncovalently bound product. Previous reports of a covalent intermediate in this enzymatic reaction probably were due to contaminating ATP in 5-phospho-alpha-D-ribose-1-diphosphate. Feedback inhibition of the enzyme by histidine was shown to occur at the step giving product or at some earlier step in the mechanism.  (+info)

trans-Recessive mutation in the first structural gene of the histidine operon that results in constitutive expression of the operon. (6/30)

The first enzyme for histidine biosynthesis, encoded in the hisG gene, is involved in regulation of expression of the histidine operon in Salmonella typhimurium. The studies reported here concern the question of how expression of the histidine operon is affected by a mutation in the hisG gene that alters the allosteric site of the first enzyme for histidine biosynthesis, rendering the enzyme completely resistant to inhibition by histidine. The intracellular concentrations of the enzymes encoded in the histidine operon in a strain carrying such a mutation on an episome and missing the chromosomal hisG gene are three- to fourfold higher than in a strain carrying a wild-type hisG gene on the episome. The histidine operon on such a strain fails to derepress in response to histidine limitation and fails to repress in response to excess histidine. Furthermore, utilizing other merodiploid strains, we demonstrate that the wild-type hisG gene is trans dominant to the mutant allele with respect to this regulatory phenomenon. Examination of the regulation of the histidine operon in strains carrying the feedback-resistant mutation in an episome and hisT and hisW mutations in the chromosome showed that the hisG regulatory mutation is epistatic to the hisT and hisW mutations. These data provide additional evidence that the first enzyme for histidine biosynthesis is involved in autogenous regulation of expression of the histidine operon.  (+info)

Derepression and repression of the histidine operon: role of the feedback site of the first enzyme. (7/30)

Thiazolealanine, a false feedback inhibitor, causes transient repression of the his operon previously derepressed by a severe histidine limitation in strains with a wild-type or feedback-hypersensitive first enzyme but not in feedback-resistant mutants. Since experiments reported here clearly demonstrate that thiazolealanine is not transferred to tRNAHis, it is proposed that this "transient repression" is effected through the interaction of thiazolealanine with the feedback site of the enzyme. Experiments in the presence of rifampin indicate that this thiazolealanine-mediated effect is exerted at the level of translation. We conclude that histidine (free), in addition to forming co-repressor, also represses the operon at the level of translation through feedback interaction with the first enzyme of the pathway (adenosine 5'-triphosphate phosphoribosyltransferase). Rates of derepression in feedback-resistant strains are roughly half of those observed in controls, suggesting a positive role played by a first enzyme with a normal but unoccupied feedback site. Some feedback-resistant mutants, in contrast to the wild type, were unable to exhibit derepression under histidine limitation caused by aminotriazole.  (+info)

Affinity labelling to - SH groups in adenosine - triphosphate - phosphoribosyl transferase with the dinitrophenyl group from S-dinitrophenyl-6-mercaptopurine-riboside 5'-phosphate. (8/30)

Adenosine-triphosphate-phosphoribosyl transferase from Escherichia coli reacts with S-dinitrophenyl-6-mercaptopurine-riboside 5'-phosphate. In this reaction the dinitrophenyl group becomes attached to the enzyme, while the nucleotide is split off. Most aliphatic high and low-molecular-weight-SH compounds react with the thioether in the opposite way, i.e. bind the nucleotide and split off dinitrothiophenol. It appears that the dinitrophenyl moiety of the thioether interacts with the enzyme in a specific way, and that this interaction activates the bond between the dinitrophenyl group and the sulfur atom. In support of this it was found that dinitrophenol inhibits the transferase reaction with half maximal effect at 0.4 mM. The inhibition is competitive with ATP. Dinitrophenol also competes with ATP in binding studies.  (+info)

hisG - ATP phosphoribosyltransferase - Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) - hisG gene & proteinhisG - ATP phosphoribosyltransferase - Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) - hisG gene & protein
Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N-(5-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity (By similarity).
https://uniprot.org/uniprot/P9WMN1
RCSB PDB - 5LHU: ATP Phosphoribosyltransferase from Mycobacterium tuberculosis in complex with the allosteric inhibitor L...RCSB PDB - 5LHU: ATP Phosphoribosyltransferase from Mycobacterium tuberculosis in complex with the allosteric inhibitor L...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
https://www.rcsb.org/structure/5LHU
Diamond Publications - Search ResultsDiamond Publications - Search Results
Abstract: Allosteric modulation of catalysis is a common regulatory strategy of flux-controlling biosynthetic enzymes. The enzyme ATP phosphoribosyltransferase (ATPPRT) catalyses the first reaction in histidine biosynthesis, the magnesium-dependent condensation of ATP and 5-phospho--D-ribosyl-1-pyrophosphate (PRPP) to generate N1-(5-phospho--D-ribosyl)-ATP (PRATP) and pyrophosphate (PPi). ATPPRT is allosterically inhibited by the final product of the pathway, histidine. Hetero-octameric ATPPRT consists of four catalytic subunits (HisGS) and four regulatory subunits (HisZ) engaged in intricate catalytic regulation. HisZ enhances HisGS catalysis in the absence of histidine while mediating allosteric inhibition in its presence. Here we report HisGS structures for the apoenzyme and complexes with substrates (PRPP, PRPP-ATP, PRPP-ADP), product (PRATP), and inhibitor (AMP), along with ATPPRT holoenzyme structures in complexes with substrates (PRPP, PRPP-ATP, PRPP-ADP) and product (PRATP). These ...
https://publications.diamond.ac.uk/pubman/searchpublicationsadvanced?authorId=69226&advancedsearch=1&publicationStates%5B3%5D.selected=true&publicationStates%5B4%5D.selected=true
Genetic Dissection of Histidine Biosynthesis in Arabidopsis | Plant PhysiologyGenetic Dissection of Histidine Biosynthesis in Arabidopsis | Plant Physiology
Analysis of the redundant first step in His biosynthesis catalyzed by ATP phosphoribosyl transferase required the construction of plants heterozygous for knockout alleles of both HISN1A (At1g58080) and HISN1B (At1g09795). The mutant alleles examined contained insertions near the beginning (hisn1b-1) or middle (hisn1a-1) of the coding region. Double knockouts for other redundant HISN genes could not be constructed because confirmed insertions remain to be identified for HISN5B and HISN6B. Twenty progeny plants from a selfed double heterozygote (AaBb) segregating for both hisn1a-1 (a) and hisn1b-1 (b) were PCR genotyped and screened for defects in seed and ovule development. The following plants were identified: AABb (5), AAbb (2), AaBB (5), and AaBb (8). Despite the small sample size, these results are consistent with two important conclusions supported by further studies: reduced transmission of double knockout gametes (ab) and embryo lethality of double homozygotes (aabb). No viable double ...
http://www.plantphysiol.org/node/26278.full.print
ATP-PRT Enzyme from TB Bacteria [image] | EurekAlert! Science NewsATP-PRT Enzyme from TB Bacteria [image] | EurekAlert! Science News
Ribbon representation of the structure of an enzyme known as ATP-PRT from TB bacteria (blue), bound to an allosteric activator (pink).
https://eurekalert.org/multimedia/pub/147163.php
Profile | Biosciences | University of ExeterProfile | Biosciences | University of Exeter
Mitotic spindles are primarily composed of microtubules (MTs), generated by polymerization of α- and β-Tubulin hetero-dimers [1, 2]. Tubulins undergo a series of protein folding and post-translational modifications in order to fulfill their functions [3, 4]. Defects in Tubulin polymerization dramatically affect spindle formation and disrupt chromosome segregation. We recently described a role for the product of the conserved misato (mst) gene in regulating mitotic MT generation in flies [5], but the molecular function of Mst remains unknown. Here, we use affinity purification mass spectrometry (AP-MS) to identify interacting partners of Mst in the Drosophila embryo. We demonstrate that Mst associates stoichiometrically with the hetero-octameric Tubulin Chaperone Protein-1 (TCP-1) complex, with the hetero-hexameric Tubulin Prefoldin complex, and with proteins having conserved roles in generating MT-competent Tubulin. We show that RNAi-mediated invivo depletion of any TCP-1 subunit phenocopies ...
http://biosciences.exeter.ac.uk/staff/profile/index.php?web_id=james_wakefield&tab=pubs&view=date
Regulation of protein trafficking by Ral GTPases and Exocyst in epithe by Yu-Tsan LiuRegulation of protein trafficking by Ral GTPases and Exocyst in epithe by Yu-Tsan Liu
In polarized epithelial cells, vectorial protein trafficking is important for transporting specific membrane proteins to generate distinct apical and basolateral membrane protein compositions. The Exocyst is a conserved hetero-octameric protein complex, which regulates different aspects of protein trafficking, including tethering of the Golgi-derived vesicles to target membranes. Two of the Exocyst subunits, Sec5 and Exo84, competitively bind to the small GTPases, RalA and RalB, in a GTP-dependent manner. Although Ral GTPases have been proposed to mediate assembly of Exocyst holocomplexes, we hypothesize that they actually serve to allosterically regulate Exocyst functions by promoting association or disassociation of additional factors. Previous studies have shown that active RalA, but not RalB, accelerated basolateral exocytosis of E-cadherin. In contrast, knockdown of RalB, but not RalA, disrupts endocytosis of E-cadherin. However, mechanisms by which association of Ral GTPases with Sec5 and Exo84
http://ir.uiowa.edu/etd/1873/
Botany & Plant Biology 2007 - Abstract SearchBotany & Plant Biology 2007 - Abstract Search
Metabolism. Shui, Wang [1], Guirong, Zhang [2], Wang, Hongyun [3], Ulanov, Alexander [3], Lozovaya, Vera [3], Lin, Yun [4]. A regulatory role of histidine in Arabidopsis metabolism and growth revealed by the low oil 1 mutant that corresponds to ATP-Phosphoribosyl Transferase 1, a key enzyme of histidine biosynthesis.. Amino acid sensory mechanisms are known to coordinate metabolism and growth in microbes and animals. However, evidence for their existence in plants has been elusive. In a genetic screen for Arabidopsis seed oil and protein deposition mutants, the low oil 1 (loo1) was isolated and map-based gene cloning identified a missense mutation in the first histidine biosynthetic enzyme ATP-phosphoribosyl transferase 1 (ATP-PRT1). The loo1 mutant exhibited development and growth defects throughout the entire lifespan. The severely retarded root elongation of loo1 seedlings was rescued by supplemental histidine in the growth medium. Transcript and metabolite profiling and real-time RT-PCR ...
http://www.2007.botanyconference.org/engine/search/index.php?func=detail&aid=477
Identification of novel bacterial histidine biosynthesis inhibitors using docking, ensemble rescoring, and whole-cell assays -...Identification of novel bacterial histidine biosynthesis inhibitors using docking, ensemble rescoring, and whole-cell assays -...
TY - JOUR. T1 - Identification of novel bacterial histidine biosynthesis inhibitors using docking, ensemble rescoring, and whole-cell assays. AU - Henriksen,Signe Teuber. AU - Liu,J.. AU - Estiu,G.. AU - Oltvai,Z.N.. AU - Wiest,O.. PY - 2010. Y1 - 2010. N2 - The rapid spread on multidrug-resistant strains of Staphylococcus aureus requires not just novel treatment options, but the development of faster methods for the identification of new hits for drug development. The exponentially increasing speed of computational methods makes a more extensive use in the early stages of drug discovery attractive if sufficient accuracy can be achieved. Computational target identification using systems-level methods suggested the histidine biosynthesis pathway as an attractive target against S. aureus. Potential inhibitors for the pathway were identified through docking, followed by ensemble rescoring, that is sufficiently accurate to justify immediate testing of the identified compounds by whole-cell assays, ...
http://orbit.dtu.dk/en/publications/identification-of-novel-bacterial-histidine-biosynthesis-inhibitors-using-docking-ensemble-rescoring-and-wholecell-assays
A CLUSTER OF GENES CONTROLLING THREE ENZYMES IN HISTIDINE BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE | GeneticsA CLUSTER OF GENES CONTROLLING THREE ENZYMES IN HISTIDINE BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE | Genetics
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
http://www.genetics.org/content/53/3/445
Bahler LabBahler Lab
Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via ...
http://bahlerweb.cs.ucl.ac.uk/cgi-bin/YOGY/yogy.pl?gene=YOR202W&species=S._cerevisiae&wild=No&go_term=Yes&go_final=No
Displaying operon conservation. Part A shows that the o | Open-iDisplaying operon conservation. Part A shows that the o | Open-i
Displaying operon conservation. Part A shows that the order of genes belonging to the histidine operon is well conserved in this set of proteobacterial genomes.
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2667195_1471-2105-9-536-5&req=4
Purification of DNA from Soil and Environmental Samples- NucleoSpin SoilPurification of DNA from Soil and Environmental Samples- NucleoSpin Soil
TIGR03572TIGR03572
This clade of sequences is highly similar to the HisF protein, but generally represents the second HisF homolog in the genome where the other is an authentic HisF observed in the context of a complete histidine biosynthesis operon. The similarity between these WbuZ sequences and true HisFs is such that often the closest match by BLAST of a WbuZ is a HisF. Only by making a multiple sequence alignment is the homology relationship among the WbuZ sequences made apparent. WbuZ genes are invariably observed in the presence of a homolog of the HisH protein (designated WbuY) and a proposed N-acetyl sugar amidotransferase designated in WbuX in E. coli [1], IfnA in P. aeriginosa [2] and PseA in C. jejuni [3]. Similarly, this trio of genes is invariably found in the context of saccharide biosynthesis loci. It has been shown that the WbuYZ homologs are not essential components of the activity expressed by WbuX, leading to the proposal that these to proteins provide ammonium ions to the amidotransferase when ...
http://www.jcvi.org/cgi-bin/tigrfams/HmmReportPage.cgi?acc=TIGR03572
GENETIC ANALYSIS OF THE HISTIDINE OPERON IN ESCHERICHIA COLI K12 | GeneticsGENETIC ANALYSIS OF THE HISTIDINE OPERON IN ESCHERICHIA COLI K12 | Genetics
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
http://www.genetics.org/content/66/2/219
What is a rate limiting step ?What is a rate limiting step ?
Hello, This question might seem easy, but I found it not so obvious as I thought about it more: What does rate limiting mean to you ? It is used quite often, and not only in biology. The example I am dealing with is a common one, a metabolic pathway. The simplest way to see it, is that there is not RenoughS of the first enzyme on the pathway and RplentyS of the following enzymes. When more of the first enzyme is made, the flux through the pathway increases in a direct (not necessarily linear) function of this first enzyme. Does it mean that this first enzyme is used at saturation of its substrate(s) ? If not, the element that keeps those substrate(s) constant would be the real rate limiting step by providing the right flux of substrate so as to keep it constant ! If that flux does not change, an increase in the quantity and consequently in flux of the first enzyme would have the effect of lowering the concentration of its substrate until the activity was restored to its original value ! I feel ...
http://www.bio.net/bionet/mm/bioforum/1993-November/006516.html
The Impact of Steroidal Glycoalkaloids on the Physiology of Phytophthora infestans, the Causative Agent of Potato Late BlightThe Impact of Steroidal Glycoalkaloids on the Physiology of Phytophthora infestans, the Causative Agent of Potato Late Blight
Steroidal glycoalkaloids (SGAs) are plant secondary metabolites known to be toxic to animals and humans and that have putative roles in defense against pests. The proposed mechanisms of SGA toxicity are sterol-mediated disruption of membranes and inhibition of cholinesterase activity in neurons. It has been suggested that phytopathogenic microorganisms can overcome SGA toxicity by enzymatic deglycosylation of SGAs. Here, we have explored SGA-mediated toxicity toward the invasive oomycete Phytophthora infestans, the causative agent of the late blight disease in potato and tomato, as well as the potential for SGA deglycosylation by this species. Our growth studies indicate that solanidine, the nonglycosylated precursor of the potato SGAs a-chaconine and a-solanine, has a greater physiological impact than its glycosylated forms. All of these compounds were incorporated into the mycelium, but only solanidine could strongly inhibit the growth of P. infestans in liquid culture. Genes encoding several ...
http://kth.diva-portal.org/smash/record.jsf?pid=diva2:1124560
Researchers discover first enzymes to catalyze a classic organic reactionResearchers discover first enzymes to catalyze a classic organic reaction
The Tang, Garg, and Houk research groups have discovered natures natural protein catalysts (enzymes) that catalyze the Alder-ene reaction.
https://phys.org/news/2020-10-enzymes-catalyze-classic-reaction.html
Mindfulness meditation slows progression of HIV, study shows | EurekAlert! Science NewsMindfulness meditation slows progression of HIV, study shows | EurekAlert! Science News
Researchers at UCLA report that engaging in the practice of mindfulness meditation stopped the decline of CD4 T immune cells in stressed HIV-positive patients, thus slowing the progression of the disease.
https://www.eurekalert.org/pub_releases/2008-07/uoc--mms072408.php
Contrasting effects of nicotianamine synthase knockdown on zinc and nickel tolerance and accumulation in the zinc/cadmium...Contrasting effects of nicotianamine synthase knockdown on zinc and nickel tolerance and accumulation in the zinc/cadmium...
Contrasting effects of nicotianamine synthase knockdown on zinc and nickel tolerance and accumulation in the zinc/cadmium hyperaccumulator Arabidopsis halleri ...
https://curis.ku.dk/portal/da/publications/contrasting-effects-of-nicotianamine-synthase-knockdown-on-zinc-and-nickel-tolerance-and-accumulation-in-the-zinccadmium-hyperaccumulator-arabidopsis-halleri
GEAM 1899 protein (Ewingella americana) - STRING interaction networkGEAM 1899 protein (Ewingella americana) - STRING interaction network
Histidine biosynthesis bifunctional protein HisIE; Protein involved in phosphoribosyl-AMP cyclohydrolase activity, phosphoribosyl-ATP diphosphatase activity and histidine biosynthetic process; In the N-terminal section; belongs to the PRA-CH family (213 aa ...
https://string-db.org/network/910964.GEAM_1899
Ebook Investitionen, Unsicherheit Und RealoptionenEbook Investitionen, Unsicherheit Und Realoptionen
now, downstream data, issues, survivors, sites, data, and univariate representative RNAs could require purified via the ebook of outcomes. The ebook to enhance acid promoters with production is that the ECD-mTLR2 cell is full to introduce possible, there misconfigured to subscribe. respectively, ebook Investitionen, has acidic certain attB, and the network phosphoribosyltransferase is to be observed for a specifically new volume.
http://www.scoutconnection.com/wwwboard/ebook.php?q=ebook-Investitionen%2C-Unsicherheit-und-Realoptionen/
Narvon Pennsylvania Information and HotelsNarvon Pennsylvania Information and Hotels
Information for Narvon ,Pennsylvania. Find community info travel, tourist, tourist and visitor information, calendars, town and travel guides, moving guides, history.
http://www.hometownusa.com/pa/Narvon.html
Receita de mini polenta com gorgonzola - Cozinha TravessaReceita de mini polenta com gorgonzola - Cozinha Travessa
Misture todos os ingredientes da polenta, menos o gorgonzola, numa panela funda e mexa sem parar. Tempere com sal à gosto e coloque um fio de azeite para não grudar. Quando começar a engrossar mexa rapidamente e proteja com a tampa da panela, pois espira muito. Deixe ficar mais cremosa e mais mole. Se ela endurecer, coloque um pouco mais de leite e mexa. Distribua em potinhos pequenos, regue com azeite e finalize com pedacinhos de gorgonzola. Servir quentinha é gostosa, mas se de repente esfriar, não se preocupe, pois não ficará ruim ...
http://cozinhatravessa.com.br/post/receita-de-mini-polenta-com-gorgonzola/
HistidineHistidine
... by the enzyme ATP-phosphoribosyl transferase. ATP-phosphoribosyl transferase is indicated by His1 in the image. His4 gene ... ATP-phosphoribosyl transferase (shown as His1 in the image on the right). ATP-phosphoribosyl transferase is the rate ... This pathway requires energy in order to occur therefore, the presence of ATP activates the first enzyme of the pathway, ... The first reaction of histidine biosynthesis is the condensation of PRPP and adenosine triphosphate (ATP) ...
https://en.wikipedia.org/wiki/Histidine
Amino acid synthesisAmino acid synthesis
... catalyzed by ATP-phosphoribosyl transferase. Phosphoribosyl-ATP converts to phosphoribosyl-AMP (PRAMP). His4 then catalyzes the ... In the asparagine synthetase reaction, ATP is used to activate aspartate, forming β-aspartyl-AMP. Glutamine donates an ammonium ... The enzyme asparagine synthetase produces asparagine, AMP, glutamate, and pyrophosphate from aspartate, glutamine, and ATP. ... is strongly inhibited by α-ketoglutarate feedback inhibition and can be inhibited by DPNH as well high concentrations of ATP. ...
https://en.wikipedia.org/wiki/Amino_acid_synthesis
Ribose 5-phosphateRibose 5-phosphate
The step of histidine biosynthesis is the condensation of ATP and PRPP by ATP-phosphoribosyl transferase, the rate determining ... R5P and its derivatives serve as precursors to many biomolecules, including DNA, RNA, ATP, coenzyme A, FAD (Flavin adenine ... The build up is caused by a deficiency of the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT), which leads to ... During the purine salvage pathway, phosphoribosyltransferases add PRPP to bases. PRPP also plays an important role in ...
https://en.wikipedia.org/wiki/Ribose_5-phosphate
List of MeSH codes (D08)List of MeSH codes (D08)
... anthranilate phosphoribosyltransferase MeSH D08.811.913.400.725.200 - ATP phosphoribosyltransferase MeSH D08.811.913.400. ... 725.450 - hypoxanthine phosphoribosyltransferase MeSH D08.811.913.400.725.700 - orotate phosphoribosyltransferase MeSH D08.811. ... atp citrate (pro-s)-lyase MeSH D08.811.913.050.350 - carnitine acyltransferases MeSH D08.811.913.050.350.170 - carnitine O- ... atp-dependent proteases MeSH D08.811.277.656.149.200 - endopeptidase clp MeSH D08.811.277.656.149.500 - protease la MeSH ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D08)
Housekeeping geneHousekeeping gene
... trafficking GUK1 Guanylate kinase transfers phosphate from ATP to GMP HPRT Hypoxanthine-guanine phosphoribosyltransferase ... 001689 Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c ATP5H NM_006356 Homo sapiens ATP ... ATP reservoir) Cytidine deaminase questionable: not present in very high levels at all CPNE1 ENSA (gene) FTH1 Heavy chain of ... 004046 Homo sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, alpha ATP5B NM_001686 ATP5C1 NM_005174 ATP5D NM_ ...
https://en.wikipedia.org/wiki/Housekeeping_gene
Orotate phosphoribosyltransferaseOrotate phosphoribosyltransferase
Uridylate (UMP) is later converted to UDP via phosphorylation by UMP kinase and ATP and then nucleoside diphosphate kinase ... Like other pyrimidine phosphoribosyltransferases, orotate phosphoribosyltransferase has a flexible loop that moves to position ... Orotate phosphoribosyltransferase (OPRTase) or orotic acid phosphoribosyltransferase is an enzyme involved in pyrimidine ... Pyrimidine phosphoribosyltransferases such as orotate phosphoribosyltransferase activate their substrates by forming SN1-like ...
https://en.wikipedia.org/wiki/Orotate_phosphoribosyltransferase
ATP phosphoribosyltransferaseATP phosphoribosyltransferase
... phosphoribosyl ATP synthetase, phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase, phosphoribosyl-ATP:pyrophosphate- ... an ATP phosphoribosyltransferase (EC 2.4.2.17) is an enzyme that catalyzes the chemical reaction 1-(5-phospho-D-ribosyl)-ATP + ... ATP phosphoribosyltransferase is found in two distinct forms: a long form containing two catalytic domains and a C-terminal ... Histidine biosynthesis is an energetically expensive process and ATP phosphoribosyltransferase activity is subject to control ...
https://en.wikipedia.org/wiki/ATP_phosphoribosyltransferase
Nicotinamide phosphoribosyltransferaseNicotinamide phosphoribosyltransferase
iNAMPT can also catalyze the synthesis of NMN from phosphoribosyl pyrophosphate (PRPP) when ATP is present. eNAMPT has been ... Nicotinamide phosphoribosyltransferase (NAmPRTase or NAMPT), formerly known as pre-B-cell colony-enhancing factor 1 (PBEF1) or ... Grolla AA, Travelli C, Genazzani AA, Sethi JK (2016). "Extracellular nicotinamide phosphoribosyltransferase, a new cancer ... Human Nicotinamide phosphoribosyltransferase) at the PDBe-KB. Overview of all the structural information available in the PDB ...
https://en.wikipedia.org/wiki/Nicotinamide_phosphoribosyltransferase
List of EC numbers (EC 2)List of EC numbers (EC 2)
ATP phosphoribosyltransferase EC 2.4.2.18: anthranilate phosphoribosyltransferase EC 2.4.2.19: nicotinate-nucleotide ... uracil phosphoribosyltransferase EC 2.4.2.10: orotate phosphoribosyltransferase EC 2.4.2.11: now EC 6.3.4.21 EC 2.4.2.12: ... nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase EC 2.4.2.22: xanthine phosphoribosyltransferase EC 2.4. ... adenine phosphoribosyltransferase EC 2.4.2.8: hypoxanthine phosphoribosyltransferase EC 2.4.2.9: ...
https://en.wikipedia.org/wiki/List_of_EC_numbers_(EC_2)
Nicotinate phosphoribosyltransferaseNicotinate phosphoribosyltransferase
ATP, and H2O, whereas its four products are nicotinate D-ribonucleotide, diphosphate, ADP, and phosphate. This enzyme belongs ... In enzymology, a nicotinate phosphoribosyltransferase (EC 6.3.4.21) is an enzyme that catalyzes the chemical reaction ... Kosaka A, Spivey HO, Gholson RK (1971). "Nicotinate phosphoribosyltransferase of yeast. Purification and properties". J. Biol. ... and nicotinic acid phosphoribosyltransferase. This enzyme participates in nicotinate and nicotinamide metabolism. As of late ...
https://en.wikipedia.org/wiki/Nicotinate_phosphoribosyltransferase
HAT mediumHAT medium
The synthesis of IMP, (precursor to GMP and GTP, and to AMP and ATP) also requires THF, and also can be bypassed. In this case ... hypoxanthine-guanine phosphoribosyltransferase (HGPRT) reacts hypoxanthine absorbed from the medium with PRPP, liberating ...
https://en.wikipedia.org/wiki/HAT_medium
Nucleic acid metabolismNucleic acid metabolism
ATP stimulates production of GTP, while GTP stimulates production of ATP. This cross regulation keeps the relative amounts of ... Lesch-Nyhan syndrome is caused by a deficiency in hypoxanthine-guanine phosphoribosyltransferase or HGPRT, the enzyme that ... ATP, a purine nucleotide, is an activator of pyrimidine synthesis, while CTP, a pyrimidine nucleotide, is an inhibitor of ... XMP is then converted into GMP by using the hydrolysis of 1 ATP and the conversion of glutamine to glutamate. AMP and GMP can ...
https://en.wikipedia.org/wiki/Nucleic_acid_metabolism
NucleotideNucleotide
Both steps are fueled by ATP hydrolysis: ATP + UMP → ADP + UDP UDP + ATP → UTP + ADP CTP is subsequently formed by the ... Orotate phosphoribosyltransferase (PRPP transferase) catalyzes the net reaction yielding orotidine monophosphate (OMP): Orotate ... Glutamine is the NH3 donor and the reaction is fueled by ATP hydrolysis, too: UTP + Glutamine + ATP + H2O → CTP + ADP + Pi ... to PRPP by reacting it with ATP. The reaction is unusual in that a pyrophosphoryl group is directly transferred from ATP to C1 ...
https://en.wikipedia.org/wiki/Nucleotide
List of EC numbers (EC 6)List of EC numbers (EC 6)
ATP) EC 6.5.1.2: DNA ligase (NAD+) EC 6.5.1.3: RNA ligase (ATP) EC 6.5.1.4: RNA-3'-phosphate cyclase EC 6.6.1.1: magnesium ... nicotinate phosphoribosyltransferase EC 6.3.5.1: NAD+ synthase (glutamine-hydrolysing) EC 6.3.5.2: GMP synthase (glutamine- ... ATP-hydrolysing)) ligase EC 6.3.4.11: biotin-(methylcrotonoyl-CoA-carboxylase) ligase EC 6.3.4.12: glutamate-methylamine ligase ...
https://en.wikipedia.org/wiki/List_of_EC_numbers_(EC_6)
GlycosomeGlycosome
Importantly, the process in the organelle has no net ATP synthesis. This ATP comes later from processes outside of the ... These enzymes found in the glycosome to help with synthesis are guanine and adenine phosphoribosyl transferase, hypoxanthine, ... This energy metabolism generates ATP through the process of glycolysis. The glycosome is a host of the main glycolytic enzymes ... the glycosomes attached to the myofibrils seem to serve the myosin by providing energy substrates for generation of ATP through ...
https://en.wikipedia.org/wiki/Glycosome
Pyrimidine metabolismPyrimidine metabolism
Conversely, PRPP and ATP act as positive effectors that enhance the enzyme's activity. Modulating the pyrimidine metabolism ... UMPS is a bifunctional enzyme consisting of orotate phosphoribosyltransferase (OPRT) and orotidine monophosphate decarboxylase ...
https://en.wikipedia.org/wiki/Pyrimidine_metabolism
Nicotinamide adenine dinucleotideNicotinamide adenine dinucleotide
This contrasts with eukaryotic DNA ligases, which use ATP to form the DNA-AMP intermediate. Li et al. have found that NAD+ ... The first step, and the rate-limiting enzyme in the salvage pathway is nicotinamide phosphoribosyltransferase (NAMPT), which ... Yamboliev IA, Smyth LM, Durnin L, Dai Y, Mutafova-Yambolieva VN (2009). "Storage and secretion of beta-NAD, ATP and dopamine in ... In most organisms, this enzyme uses ATP as the source of the phosphate group, although several bacteria such as Mycobacterium ...
https://en.wikipedia.org/wiki/Nicotinamide_adenine_dinucleotide
Purine metabolismPurine metabolism
fGAR + L-Glutamine + ATP → fGAM + L-Glutamate + ADP + Pi The fifth is catalyzed by AIR synthetase (FGAM cyclase). fGAM + ATP → ... The enzyme adenine phosphoribosyltransferase (APRT) salvages adenine. The enzyme hypoxanthine-guanine phosphoribosyltransferase ... CAIR + L-Aspartate + ATP → SAICAR + ADP + Pi The eight is catalyzed by adenylosuccinate lyase. SAICAR → AICAR + Fumarate The ... PRPP + L-Glutamine + H2O → PRA + L-Glutamate + PPi In the second step react PRA, glycine and ATP to create GAR, ADP, and ...
https://en.wikipedia.org/wiki/Purine_metabolism
Nucleotide salvageNucleotide salvage
hypoxanthine/guanine phosphoribosyl transferase (HGPRT). IMP guanine. hypoxanthine/guanine phosphoribosyl transferase (HGPRT). ... Glycolysis → Pyruvate decarboxylation → Citric acid cycle → Oxidative phosphorylation (electron transport chain + ATP synthase) ... There are two types of phosphoribosyltransferases: adenine phosphoribosyltransferase (APRT) and hypoxanthine-guanine ... adenine phosphoribosyltransferase (APRT). AMP Folate biosynthesis[edit]. Tetrahydrofolic acid and its derivatives are produced ...
https://en.wikipedia.org/wiki/Nucleotide_salvage
BiosynthesisBiosynthesis
These may be metal ions, vitamin derivatives such as NADH and acetyl CoA, or non-vitamin derivatives such as ATP. In the case ... It is caused by the absence of hypoxanthine-guanine phosphoribosyltransferase, which is a necessary enzyme for purine ... The first step for joining an amino acid to its corresponding tRNA is the formation of aminoacyl-AMP: Amino acid + ATP ↽ − − ⇀ ... The following step requires the activation of glycine by the addition of a phosphate group from ATP. GAR synthetase performs ...
https://en.wikipedia.org/wiki/Biosynthesis
List of enzymesList of enzymes
ATP synthase (EC 3.6.3.14) Kynureninase EC 3.7.1.3 EC 3.8.1.3 Haloacetate dehalogenase EC 3.9.1.1: Phosphoamidase EC 3.9.1.2: ... EC 2.4.2 Hypoxanthine-guanine phosphoribosyltransferase EC 2.4.2.8 Category:EC 2.5 Category:EC 2.5.1 Thiaminase EC 2.5.1.2 ... ATP-hydrolysing) Serine racemase Category:EC 5.1.2 Mandelate racemase Category:EC 5.1.3 UDP-glucose 4-epimerase Category:EC 5.1 ...
https://en.wikipedia.org/wiki/List_of_enzymes
MorpheeinMorpheein
Functional Consequences of ATP- and dATP-Induced Oligomerization of the Large Subunit†". Biochemistry. 41 (2): 462-74. doi: ... "Different Oligomeric States are Involved in the Allosteric Behavior of Uracil Phosphoribosyltransferase from Escherichia Coli ... W in Complex with Nonhydrolyzable ATP Analogues Reveal a Putative Active Conformation of the Enzyme as a Result of Domain ... Dependent Malic Enzyme by ATP and Fumarate". Structure. 10 (7): 951-60. doi:10.1016/S0969-2126(02)00788-8. PMID 12121650. ...
https://en.wikipedia.org/wiki/Morpheein
UCK2UCK2
Alternatively to ATP, GTP has been shown to act comparably as a phosphate donor. This promiscuity enables the important role ... "Epstein-Barr virus encoded nuclear protein EBNA-3 binds a novel human uridine kinase/uracil phosphoribosyltransferase". BMC ... hydroxyl group on the substrate and activates it to attack the γ-phosphorus of ATP. Structural analyses have shown that the ...
https://en.wikipedia.org/wiki/UCK2
Sucrose phosphorylaseSucrose phosphorylase
... the presence of ATP would probably inhibit sucrose phosphorylase since ATP is a product of the catabolic pathway. Conversely, ... Phosphoribosyltransferase. *Adenine phosphoribosyltransferase. *Hypoxanthine-guanine phosphoribosyltransferase. *Uracil ... ADP would likely stimulate sucrose phosphorylase to increase levels of ATP. Further research on the subject would be required ...
https://en.wikipedia.org/wiki/Sucrose_phosphorylase
Glycogen phosphorylaseGlycogen phosphorylase
An increase in ATP concentration opposes this activation by displacing AMP from the nucleotide binding site, indicating ... Phosphoribosyltransferase. *Adenine phosphoribosyltransferase. *Hypoxanthine-guanine phosphoribosyltransferase. *Uracil ... Phosphorylase b is normally in the T state, inactive due to the physiological presence of ATP and Glucose 6 phosphate, and ...
https://en.wikipedia.org/wiki/Glycogen_phosphorylase
Cholera toxinCholera toxin
... which leads to ATP-mediated efflux of chloride ions and leads to secretion of H2O, Na+, K+, and HCO3− into the intestinal lumen ... Phosphoribosyltransferase. *Adenine phosphoribosyltransferase. *Hypoxanthine-guanine phosphoribosyltransferase. *Uracil ...
https://en.wikipedia.org/wiki/Cholera_toxin
List of enzymesList of enzymes
ATP synthase (EC 3.6.3.14). Category:EC 3.7 (act on carbon-carbon bonds)Edit. *Kynureninase EC 3.7.1.3 ... Hypoxanthine-guanine phosphoribosyltransferase EC 2.4.2.8. Category:EC 2.5 *Category:EC 2.5.1 *Thiaminase EC 2.5.1.2 ...
https://en.m.wikipedia.org/wiki/List_of_enzymes
HyperuricemiaHyperuricemia
This interference has a dual action, both increasing the conversion of ATP to inosine and hence uric acid and increasing the ... Adenine phosphoribosyltransferase deficiency. Catabolism. *Adenosine deaminase deficiency. *Purine nucleoside phosphorylase ...
https://en.wikipedia.org/wiki/Hyperuricemia
HistidineHistidine
ATP-phosphoribosyl transferase (shown as His1 in the image on the right). ATP-phosphoribosyl transferase is the rate ... by the enzyme ATP-phosphoribosyl transferase. ATP-phosphoribosyl tranferase is indicated by His1 in the image.[13] His4 gene ... This pathway requires energy in order to occur therefore, the presence of ATP activates the first enzyme of the pathway, ... The first reaction of histidine biosynthesis is the condensation of PRPP and adenosine triphosphate (ATP) ...
https://en.wikipedia.org/wiki/L-Histidine
ATP phosphoribosyltransferase - WikipediaATP phosphoribosyltransferase - Wikipedia
... phosphoribosyl ATP synthetase, phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase, phosphoribosyl-ATP:pyrophosphate- ... an ATP phosphoribosyltransferase (EC 2.4.2.17) is an enzyme that catalyzes the chemical reaction 1-(5-phospho-D-ribosyl)-ATP + ... ATP phosphoribosyltransferase is found in two distinct forms: a long form containing two catalytic domains and a C-terminal ... Histidine biosynthesis is an energetically expensive process and ATP phosphoribosyltransferase activity is subject to control ...
https://en.wikipedia.org/wiki/ATP_phosphoribosyltransferase
hisG ATP phosphoribosyltransferase [Desulfovibrio vulgaris str. Hildenborough] - Gene - NCBIhisG ATP phosphoribosyltransferase [Desulfovibrio vulgaris str. Hildenborough] - Gene - NCBI
hisG; ATP phosphoribosyltransferase; Reviewed. TIGR03455. Location:202 → 293. HisG_C-term; ATP phosphoribosyltransferase, C- ... ATP phosphoribosyltransferase. YP_009339.1. *EC 2.4.2.17. *long form of enzyme; catalyzes the formation of N-5-phosphoribosyl ... ATP phosphoribosyltransferase. Locus tag. DVU0114. Gene type. protein coding. RefSeq status. REVIEWED. Organism. Desulfovibrio ... hisG ATP phosphoribosyltransferase [ Desulfovibrio vulgaris str. Hildenborough ] Gene ID: 2794983, updated on 29-Aug-2016 ...
https://www.ncbi.nlm.nih.gov/gene/2794983
hisG ATP phosphoribosyltransferase [Streptococcus mutans UA159] - Gene - NCBIhisG ATP phosphoribosyltransferase [Streptococcus mutans UA159] - Gene - NCBI
ATP phosphoribosyltransferase. NP_721647.1. *Best Blastp Hit: sp,Q02129,HIS1_LACLA ATP PHOSPHORIBOSYLTRANSFERASE ,gi,478062,pir ... ATP phosphoribosyltransferase. Locus tag. SMU_1271. Gene type. protein coding. RefSeq status. PROVISIONAL. Organism. ... NP_721647.1 ATP phosphoribosyltransferase [Streptococcus mutans UA159]. See identical proteins and their annotated locations ... hisG ATP phosphoribosyltransferase [ Streptococcus mutans UA159 ] Gene ID: 1028559, updated on 30-Jan-2018 ...
https://www.ncbi.nlm.nih.gov/gene?term=SMU.1271
RCSB PDB - 1H3D: STRUCTURE OF THE E.COLI ATP-PHOSPHORIBOSYLTRANSFERASE Macromolecule Annotations PageRCSB PDB - 1H3D: STRUCTURE OF THE E.COLI ATP-PHOSPHORIBOSYLTRANSFERASE Macromolecule Annotations Page
The Structure of Escherichia Coli ATP-Phosphoribosyltransferase: Identification of Substrate Binding Sites and Mode of AMP ... ATP phosphoribosyltransferase (ATP-PRTase, HisG), regulatory C- terminal domain ATP phosphoribosyltransferase (ATP-PRTase, HisG ... ATP phosphoribosyltransferase (ATP-PRTase, HisG), catalytic domain Escherichia coli [TaxId: 562] A225-299. d1h3da2. Alpha and ...
http://www.rcsb.org/pdb/explore/derivedData.do?structureId=1H3D
hisG - ATP phosphoribosyltransferase - Mycobacterium bovis (strain BCG / Pasteur 1173P2) - hisG gene & proteinhisG - ATP phosphoribosyltransferase - Mycobacterium bovis (strain BCG / Pasteur 1173P2) - hisG gene & protein
ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by ... Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N-(5-phosphoribosyl)- ... IPR013820, ATP_PRibTrfase_cat. IPR018198, ATP_PRibTrfase_CS. IPR001348, ATP_PRibTrfase_HisG. IPR020621, ATP_PRibTrfase_HisG ... IPR013820, ATP_PRibTrfase_cat. IPR018198, ATP_PRibTrfase_CS. IPR001348, ATP_PRibTrfase_HisG. IPR020621, ATP_PRibTrfase_HisG ...
https://www.uniprot.org/uniprot/A1KKG4
hisG - ATP phosphoribosyltransferase - Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) - hisG gene & proteinhisG - ATP phosphoribosyltransferase - Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) - hisG gene & protein
ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by ... Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N-(5-phosphoribosyl)- ... IPR013820 ATP_PRibTrfase_cat. IPR018198 ATP_PRibTrfase_CS. IPR001348 ATP_PRibTrfase_HisG. IPR020621 ATP_PRibTrfase_HisG_long. ... IPR013820 ATP_PRibTrfase_cat. IPR018198 ATP_PRibTrfase_CS. IPR001348 ATP_PRibTrfase_HisG. IPR020621 ATP_PRibTrfase_HisG_long. ...
https://uniprot.org/uniprot/P9WMN1
RCSB PDB - 5LHU: ATP Phosphoribosyltransferase from Mycobacterium tuberculosis in complex with the allosteric inhibitor L...RCSB PDB - 5LHU: ATP Phosphoribosyltransferase from Mycobacterium tuberculosis in complex with the allosteric inhibitor L...
ATP-phosphoribosyltransferase (ATP-PRT) is a hexameric enzyme in conformational equilibrium between an open and seemingly ... ATP-phosphoribosyltransferase (ATP-PRT) is a hexameric enzyme in conformational equilibrium between an open and seemingly ... ATP phosphoribosyltransferase. A. 294. Mycobacterium tuberculosis H37Rv. Mutation(s): 0 Gene Names: hisG, Rv2121c, MTCY261.17c ... ATP Phosphoribosyltransferase from Mycobacterium tuberculosis in complex with the allosteric inhibitor L-Histidine. *DOI: ...
https://www.rcsb.org/structure/5LHU
Nicotinamide Phosphoribosyltransferase Deficiency Potentiates the Antiproliferative Activity of Methotrexate through Enhanced...Nicotinamide Phosphoribosyltransferase Deficiency Potentiates the Antiproliferative Activity of Methotrexate through Enhanced...
NAMPT Deficiency Potentiates ATP Depletion by Methotrexate. Rakesh K. Singh, Leon van Haandel, Daniel P. Heruth, Shui Q. Ye, J ... NAMPT Deficiency Potentiates ATP Depletion by Methotrexate. Rakesh K. Singh, Leon van Haandel, Daniel P. Heruth, Shui Q. Ye, J ... Lower plasma nicotinamide phosphoribosyltransferase (NAMPT) levels are associated with improved response to methotrexate (MTX) ... Nicotinamide Phosphoribosyltransferase Deficiency Potentiates the Antiproliferative Activity of Methotrexate through Enhanced ...
http://jpet.aspetjournals.org/content/365/1/96
Recombinant Escherichia coli O6:K15:H31 ATP phosphoribosyltransferase(hisG) - CusabioRecombinant Escherichia coli O6:K15:H31 ATP phosphoribosyltransferase(hisG) - Cusabio
H31 ATP phosphoribosyltransferase(hisG). It is produced in Yeast. High purity. Good price. ... Recommended name: ATP phosphoribosyltransferase Short name= ATP-PRT Short name= ATP-PRTase EC= 2.4.2.17 ... Recombinant Escherichia coli O6:K15:H31 ATP phosphoribosyltransferase(hisG). Recombinant Escherichia coli O6:K15:H31 ATP ... Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N-(5-phosphoribosyl)-ATP (PR-ATP). Has a crucial ...
https://www.cusabio.com/Recombinant-Protein/Recombinant-Escherichia-coli-O6:K15:H31-ATP-phosphoribosyltransferasehisG-685269.html
Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like...Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like...
Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like ... The molecular structure of the ATP phosphoribosyl transferase from the hyperthermophile Thermotoga maritima is composed of a ... The molecular structure of the ATP phosphoribosyl transferase from the hyperthermophile Thermotoga maritima is composed of a ... 2 ATP phosphoribosyl transferases that is functional in the absence of additional regulatory subunits, the structure of the ...
https://epub.uni-regensburg.de/13678/
ATP Phosphoribosyltransferase | Profiles RNSATP Phosphoribosyltransferase | Profiles RNS
"ATP Phosphoribosyltransferase" by people in this website by year, and whether "ATP Phosphoribosyltransferase" was a major or ... "ATP Phosphoribosyltransferase" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ... ATP reacts reversibly with 5-phosphoribosyl-1-pyrophosphate to yield N-1-(5-phosphoribosyl)-ATP and pyrophosphate. EC 2.4.2.17 ... Below are the most recent publications written about "ATP Phosphoribosyltransferase" by people in Profiles. ...
https://researchers.childrensmercy.org/display/3934
Bacillus pumilus ATP phosphoribosyltransferase (hisG) GENTAUR-58ba9e4ae2c25 | GentaursearchBacillus pumilus ATP phosphoribosyltransferase (hisG) GENTAUR-58ba9e4ae2c25 | Gentaursearch
Bacillus pumilus ATP phosphoribosyltransferase (hisG) datasheet and description hight quality product and Backed by our ... ATP phosphoribosyltransferase, ATP phosphoribosyltransferase catalytic subunit, ATP phosphoribosyltransferase catalytic subunit ... Bacillus pumilus ATP phosphoribosyltransferase (hisG). Alternative name: Bacillus pumilus adenosine triphosphate ... ATP-PRT, ATP-PRTase, hisG, hisG. General description: All of our recombinant proteins are manufactured in strictly controlled ...
http://gentaursearch.com/product/human/1249493/bacillus-pumilus-atp-phosphoribosyltransferase-hisg.html
Kinetics and structure of a cold-adapted hetero-octameric ATP phosphoribosyltransferaseKinetics and structure of a cold-adapted hetero-octameric ATP phosphoribosyltransferase
... the condensation of ATP and 5-phospho-α-d-ribosyl-1-pyrophosphate to generate N1-(5-phospho-β-d-ribosyl)-ATP and inorganic ... Kinetics and structure of a cold-adapted hetero-octameric ATP phosphoribosyltransferase , Biochemistry , vol. 56 , no. 5 , pp ... Adenosine 5′-triphosphate phosphoribosyltransferase (ATPPRT) catalyzes the first step in histidine biosynthesis, ...
https://research-repository.st-andrews.ac.uk/handle/10023/12496
B2TYG1 | SWISS-MODEL RepositoryB2TYG1 | SWISS-MODEL Repository
ATP phosphoribosyltransferase. Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) ... Structure of ATP-phosphoribosyltransferase from E. coli complexed with PR-ATP. homo-6-mer. 6×PRT; 12×TLA;. 1q1k*SMTL ... ATP phosphoribosyltransferase UniProtKBInterProInteractive Modelling. 299 aa; Sequence (Fasta) ; 147 identical sequences 147 ... STRUCTURE OF THE E.COLI ATP-PHOSPHORIBOSYLTRANSFERASE. homo-6-mer. 6×AMP; 12×TLA;. 1h3d*SMTL ...
https://swissmodel.expasy.org/repository/uniprot/B2TYG1
A1AWP5 | SWISS-MODEL RepositoryA1AWP5 | SWISS-MODEL Repository
ATP phosphoribosyltransferase UniProtKBInterProSTRINGInteractive Modelling. 205 aa; Sequence (Fasta) It is possible new ... ATP phosphoribosyltransferase. Ruthia magnifica subsp Calyptogena magnifica ...
https://swissmodel.expasy.org/repository/uniprot/A1AWP5
IUCr) Acta Crystallographica Section D Volume 56, Part 11, November 2000IUCr) Acta Crystallographica Section D Volume 56, Part 11, November 2000
Crystals of ATP-phosphoribosyltransferase from E. coli were obtained by the vapour-diffusion method and diffract to 2.7 Å. The ... Purification, crystallization and preliminary X-ray crystallographic analysis of ATP-phosphoribosyltransferase from Escherichia ...
http://journals.iucr.org/d/issues/2000/11/00/
hisC - Histidinol-phosphate aminotransferase - Chlorobium luteolum (strain DSM 273 / 2530) - hisC gene & proteinhisC - Histidinol-phosphate aminotransferase - Chlorobium luteolum (strain DSM 273 / 2530) - hisC gene & protein
ATP phosphoribosyltransferase (hisG). *no protein annotated in this organism. *Phosphoribosyl-AMP cyclohydrolase (hisI) ...
https://www.uniprot.org/uniprot/Q3B3L3
New Schizosaccharomyces pombe entriesNew Schizosaccharomyces pombe entries
KW ATP phosphoribosyltransferase; bZIP transcription factor; cut7; KW his1; histidine biosynthesis; kinesin; rec10; ribosomal ...
http://bio.net/bionet/mm/yeast/1996-June/005363.html
Engineering and application of a biosensor with focused ligand specificity | Nature CommunicationsEngineering and application of a biosensor with focused ligand specificity | Nature Communications
5: Amino acid substitutions in the ATP-phosphoribosyltransferase (HisG).. Location of the amino acid substitutions in the ... Cho, Y., Sharma, V. & Sacchettini, J. C. Crystal structure of ATP phosphoribosyltransferase from Mycobacterium tuberculosis. J ... from 0.4 to 0.7 mM revealed that all variants bear mutations in the hisG gene encoding for the ATP-phosphoribosyltransferase ( ...
https://www.nature.com/articles/s41467-020-18400-0?error=cookies_not_supported&code=c1179a86-07e7-4978-8365-af4155164436
KEGG BRITE: KEGG Orthology (KO) - Acinetobacter baumannii AYEKEGG BRITE: KEGG Orthology (KO) - Acinetobacter baumannii AYE
K00765 hisG; ATP phosphoribosyltransferase [EC:2.4.2.17] K02502 hisZ; ATP phosphoribosyltransferase regulatory subunit K11755 ... ABAYE3132 hisG; ATP-phosphoribosyltransferase ABAYE2593 hisZ; ATP phosphoribosyltransferase ABAYE3428 hisIE; bifunctional ... ATP) [EC:2.7.1.107] K00635 E2.3.1.20; diacylglycerol O-acyltransferase [EC:2.3.1.20] K01046 E3.1.1.3; triacylglycerol lipase [ ... hisIE; phosphoribosyl-ATP pyrophosphohydrolase / phosphoribosyl-AMP cyclohydrolase [EC:3.6.1.31 3.5.4.19] K01814 hisA; ...
http://www.genome.jp/kegg-bin/get_htext?aby00001+ABAYE2333
Geochemical Stability of Dissolved Mn(III) in the Presence of Pyrophosphate as a Model Ligand: Complexation and...Geochemical Stability of Dissolved Mn(III) in the Presence of Pyrophosphate as a Model Ligand: Complexation and...
Atp Phosphoribosyltransferase. An enzyme that catalyzes the first step of the pathway for histidine biosynthesis in Salmonella ... ATP reacts reversibly with 5-phosphoribosyl-1-pyrophosphate to yield N-1-(5-phosphoribosyl)-ATP and pyrophosphate. EC 2.4.2.17 ...
https://www.bioportfolio.com/resources/pmarticle/2342572/Geochemical-Stability-of-Dissolved-Mn-III-in-the-Presence-of-Pyrophosphate-as.html
Baicalin protects mice against Salmonella typhimurium infection via the modulation of both bacterial virulence and host...Baicalin protects mice against Salmonella typhimurium infection via the modulation of both bacterial virulence and host...
Atp Phosphoribosyltransferase. An enzyme that catalyzes the first step of the pathway for histidine biosynthesis in Salmonella ... ATP reacts reversibly with 5-phosphoribosyl-1-pyrophosphate to yield N-1-(5-phosphoribosyl)-ATP and pyrophosphate. EC 2.4.2.17 ...
https://www.bioportfolio.com/resources/pmarticle/2151636/Baicalin-protects-mice-against-Salmonella-typhimurium-infection-via-the-modulation-of-both.html
SWISSPROT: HIS1 ANADESWISSPROT: HIS1 ANADE
DE RecName: Full=ATP phosphoribosyltransferase {ECO:0000255,HAMAP-Rule:MF_01018}; DE Short=ATP-PRT {ECO:0000255,HAMAP-Rule:MF_ ... DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0003879; F:ATP phosphoribosyltransferase activity; IEA:UniProtKB- ... DR InterPro; IPR018198; ATP_PRibTrfase_CS. DR InterPro; IPR001348; ATP_PRibTrfase_HisG. DR InterPro; IPR024893; ATP_PRibTrfase_ ... CC -!- SIMILARITY: Belongs to the ATP phosphoribosyltransferase family. CC Short subfamily. {ECO:0000255,HAMAP-Rule:MF_01018}. ...
http://pbil.univ-lyon1.fr/cgi-bin/acnuc-search-ac?query=ABC80139&db=SWISSPROT&ident=ACNUC7421
Site - hisGSite - hisG
ATP phosphoribosyltransferase. *Direction: >*Properties:. Property. Comment. auxotrophy-amino acid. NULL *Priority: 1. *4 ...
https://cgsc.biology.yale.edu/Site.php?ID=630
Histidine - WikipediaHistidine - Wikipedia
... by the enzyme ATP-phosphoribosyl transferase. ATP-phosphoribosyl transferase is indicated by His1 in the image. His4 gene ... ATP-phosphoribosyl transferase (shown as His1 in the image on the right). ATP-phosphoribosyl transferase is the rate ... This pathway requires energy in order to occur therefore, the presence of ATP activates the first enzyme of the pathway, ... The first reaction of histidine biosynthesis is the condensation of PRPP and adenosine triphosphate (ATP) ...
https://en.wikipedia.org/wiki/Histidine
Amino acid synthesis - WikipediaAmino acid synthesis - Wikipedia
... catalyzed by ATP-phosphoribosyl transferase. Phosphoribosyl-ATP converts to phosphoribosyl-AMP (PRAMP). His4 then catalyzes the ... The enzyme asparagine synthetase produces asparagine, AMP, glutamate, and pyrophosphate from aspartate, glutamine, and ATP. In ... Glycolysis → Pyruvate decarboxylation → Citric acid cycle → Oxidative phosphorylation (electron transport chain + ATP synthase) ... is strongly inhibited by α-ketoglutarate feedback inhibition and can be inhibited by DPNH as well high concentrations of ATP.[5 ...
https://en.wikipedia.org/wiki/Amino_acid_synthesis
Figures and data in Comparative genomics explains the evolutionary success of reef-forming corals | eLifeFigures and data in Comparative genomics explains the evolutionary success of reef-forming corals | eLife
atp phosphoribosyltransferase. 100. CA/Fungi. 11. Coral. Seriatopora_8296. glyoxalase. 98. Bact. ... Maximum likelihood tree of an exonuclease-endonucease-phosphatase (EEP) domain-containing protein (A), an ATP-dependent ...
https://elifesciences.org/articles/13288/figures
EC 2.4.2EC 2.4.2
Accepted name: ATP phosphoribosyltransferase Reaction: 1-(5-phospho-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-α-D-ribose 1 ... phosphoribosyl ATP synthetase; phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase; phosphoribosyl-ATP:pyrophosphate- ... EC 2.4.2.17 ATP phosphoribosyltransferase EC 2.4.2.18 anthranilate phosphoribosyltransferase EC 2.4.2.19 nicotinate-nucleotide ... Systematic name: ATP:3-dephospho-CoA 5-triphosphoribosyltransferase. Comments: ATP cannot be replaced by GTP, CTP, UTP, ADP or ...
https://www.qmul.ac.uk/sbcs/iubmb/enzyme/EC2/0402.html
Physiological and pathophysiological roles of NAMPT and NAD metabolism | Nature Reviews EndocrinologyPhysiological and pathophysiological roles of NAMPT and NAD metabolism | Nature Reviews Endocrinology
Nicotinamide phosphoribosyltransferase (NAMPT) is a regulator of the intracellular nicotinamide adenine dinucleotide (NAD) pool ... Nicotinamide phosphoribosyltransferase (NAMPT) regulates the biosynthesis of nicotinamide adenine dinucleotide (NAD), levels of ... Schilling, E. & Hauschildt, S. Extracellular ATP induces P2X7-dependent nicotinamide phosphoribosyltransferase release in LPS- ... Weak coupling of ATP hydrolysis to the chemical equilibrium of human nicotinamide phosphoribosyltransferase. Biochemistry 47, ...
https://www.nature.com/articles/nrendo.2015.117?error=cookies_not_supported&code=f3299256-047d-4541-872d-6a5b5eae5299
Samantha Campbell CaseySamantha Campbell Casey
ATP-phosphoribosyltransferase (ATP-PRT), the first enzyme of the histidine pathway, is a complex allosterically regulated ... The Structure of Escherichia coli ATP-phosphoribosyltransferase: Identification of Substrate Binding Sites and Mode of AMP ... The Structure of Escherichia coli ATP-phosphoribosyltransferase: Identification of Substrate Binding Sites and Mode of AMP ... The Structure of Escherichia coli ATP-phosphoribosyltransferase: Identification of Substrate Binding Sites and Mode of AMP ...
https://www.napier.ac.uk/people/samantha-campbell-casey
HisG ATP phosphoribosyltransferaseBiosynthesisAdenosine triphosphate phosphoribosyltransferaseSubunitNicotinamideNAMPTAdenine phosphoribosyltransferase2.17Belongs to the ATPProteinMRNACatalyzesHetero-octamericEnzymesPyrophosphateMycobacterium TuberculosisHexamericGeneAllostericEnergetically expensiveSynthaseDiphosphateHGPRTHypoxanthineMetabolismCatalysesReactionRegulatoryRegulationAmino acidOxidative
HisG ATP phosphoribosyltransferase1
  • hisG ATP phosphoribosyltransferase [Desulfovibrio vulgaris str. (nih.gov)
Biosynthesis6
  • Histidine biosynthesis is an energetically expensive process and ATP phosphoribosyltransferase activity is subject to control at several levels. (wikipedia.org)
  • Adenosine 5′-triphosphate phosphoribosyltransferase (ATPPRT) catalyzes the first step in histidine biosynthesis, the condensation of ATP and 5-phospho-α-d-ribosyl-1-pyrophosphate to generate N1-(5-phospho-β-d-ribosyl)-ATP and inorganic pyrophosphate. (st-andrews.ac.uk)
  • Pre-B-cell colony-enhancing factor, whose expression is up-regulated in activated lymphocytes, is a nicotinamide phosphoribosyltransferase, a cytosolic enzyme involved in NAD biosynthesis. (nature.com)
  • His biosynthesis (black) and related ATP synthesis (blue, red) and recycling (blue) pathways in Arabidopsis. (plantphysiol.org)
  • A regulatory role of histidine in Arabidopsis metabolism and growth revealed by the low oil 1 mutant that corresponds to ATP-Phosphoribosyl Transferase 1, a key enzyme of histidine biosynthesis. (botanyconference.org)
  • The biosynthesis of histidine begins with the transformation of D-ribose 5-phosphate through an ATP-driven pyrophosphokinase resulting in the release of AMP, hydrogen ion, and phosphoribosyl pyrophosphate. (smpdb.ca)
Adenosine triphosphate phosphoribosyltransferase2
  • Other names in common use include phosphoribosyl-ATP pyrophosphorylase, adenosine triphosphate phosphoribosyltransferase, phosphoribosyladenosine triphosphate:pyrophosphate, phosphoribosyltransferase, phosphoribosyl ATP synthetase, phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase, phosphoribosyl-ATP:pyrophosphate-phosphoribosyl phosphotransferase, phosphoribosyladenosine triphosphate pyrophosphorylase, and phosphoribosyladenosine triphosphate synthetase. (wikipedia.org)
  • The enzyme adenosine triphosphate phosphoribosyltransferase (ATP-PRT) catalyses the first reaction of the histidine biosynthetic pathway. (canterbury.ac.nz)
Subunit3
  • Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like subunit. (uni-regensburg.de)
  • The rationale for these findings is provided by the crystal structure revealing a total of eight histidine binding sites that are located within each of the four HisGS-HisZ subunit interfaces formed by the ATP phosphoribosyl transferase complex. (uni-regensburg.de)
  • While the structure of the catalytic HisGS subunit is related to the catalytic domain of another family of (HisGL)2 ATP phosphoribosyl transferases that is functional in the absence of additional regulatory subunits, the structure of the regulatory HisZ subunit is distantly related to class II aminoacyl-tRNA synthetases. (uni-regensburg.de)
Nicotinamide6
  • Lower plasma nicotinamide phosphoribosyltransferase (NAMPT) levels are associated with improved response to methotrexate (MTX) in patients with juvenile idiopathic arthritis. (aspetjournals.org)
  • Nicotinamide phosphoribosyltransferase (NAMPT) is a regulator of the intracellular nicotinamide adenine dinucleotide (NAD) pool. (nature.com)
  • AMP-activated kinase (AMPK), which mediates anti-inflammatory effects of IL-10, and nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme of the NAD + salvage pathway, which is under the control of AMPK, were also required for P2Y 11 receptor expression. (frontiersin.org)
  • Nicotinamide phosphoribosyltransferase (NAMPT) is the rate limiting enzyme in NAD+ salvaging from nicotinamide. (aacrjournals.org)
  • Inhibitors of nicotinamide phosphoribosyltransferase (NAMPT) are promising cancer drugs currently in clinical trials in oncology, including APO866, CHS-828 and the CHS-828 prodrug EB1627/GMX1777, but cancer cell resistance to these drugs has not been studied in detail. (biomedcentral.com)
  • Nicotinamide is converted to nicotinamide mononucleotide (NMN) using 5-phosphoribosyl-1-pyrophosphate and ATP as substrates. (biomedcentral.com)
NAMPT2
  • The crystal structure of NAMPT has been resolved and it has been identified as a dimer belonging to the family of type II phosphoribosyltransferases [ 14 - 16 ] - each monomer containing two domains. (biomedcentral.com)
  • Inhibition of NAMPT leads to depletion of NAD [ 17 ], secondarily leading to reduction of ATP and later, cell death. (biomedcentral.com)
Adenine phosphoribosyltransferase1
  • The concentrations of ATP, ADP, AMP, IMP and the activities of adenine phosphoribosyltransferase (APRT), hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and phosphoribosyl pyrophosphate synthetase (PRPP-S) were determined in erythrocytes. (springer.com)
2.172
  • In enzymology, an ATP phosphoribosyltransferase (EC 2.4.2.17) is an enzyme that catalyzes the chemical reaction 1-(5-phospho-D-ribosyl)-ATP + diphosphate ⇌ {\displaystyle \rightleftharpoons } ATP + 5-phospho-alpha-D-ribose 1-diphosphate Thus, the two substrates of this enzyme are 1-(5-phospho-D-ribosyl)-ATP and diphosphate, whereas its two products are ATP and 5-phospho-alpha-D-ribose 1-diphosphate. (wikipedia.org)
  • D45734 ATP phosphoribosyltransferase (EC 2.4.2.17) [similarity] - Lactococcus lactis subsp. (nih.gov)
Belongs to the ATP1
  • SIMILARITY: Belongs to the ATP phosphoribosyltransferase family. (univ-lyon1.fr)
Protein2
  • The G protein-coupled P2Y 11 receptor is known to sense extracellular ATP during inflammatory and immune responses. (frontiersin.org)
  • In a genetic screen for Arabidopsis seed oil and protein deposition mutants, the low oil 1 ( loo1 ) was isolated and map-based gene cloning identified a missense mutation in the first histidine biosynthetic enzyme ATP-phosphoribosyl transferase 1 ( ATP-PRT1 ). (botanyconference.org)
MRNA2
  • In earlier studies, P2Y 11 receptor involvement was suggested through detection of P2Y 11 receptor-encoding mRNA, by exclusion of other possible ATP receptors, and later by siRNA knockdown of the receptor to support pharmacological data, often related to cell survival and cytokine production as well as cell migration and differentiation ( 7 - 11 ). (frontiersin.org)
  • The mRNA levels of nicotinate phosphoribosyltransferase 1 (NAPRT1), a central enzyme of the Preiss-Haendler pathway, predicted well the niacin rescue status of the cells. (aacrjournals.org)
Catalyzes4
  • Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). (uniprot.org)
  • ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesi. (yeastrc.org)
  • The N-1-(5'-phosphoribosyl)-ATP transferase catalyzes the first step of the histidine biosynthetic pathway and is regulated by a feedback mechanism by the product histidine. (tamu.edu)
  • 1], Lesch-Nyhan syndrome is caused by a deficiency in hypoxanthine-guanine phosphoribosyltransferase or HGPRT, the enzyme that catalyzes the reversible reaction of producing guanine from GMP. (youngtechies.com)
Hetero-octameric1
  • The molecular structure of the ATP phosphoribosyl transferase from the hyperthermophile Thermotoga maritima is composed of a 220 kDa hetero-octameric complex comprising four catalytic subunits (HisGS) and four regulatory subunits (HisZ). (uni-regensburg.de)
Enzymes5
  • It is a member of the larger phosphoribosyltransferase superfamily of enzymes which catalyse the condensation of 5-phospho-alpha-D-ribose 1-diphosphate with nitrogenous bases in the presence of divalent metal ions. (wikipedia.org)
  • These findings suggest that dynamic processes are responsible for ATP-PRT allosteric regulation and that similar mechanisms might also be found in other enzymes bearing a ferredoxin-like allosteric domain.Active and inactive state ATP-phosphoribosyltransferases (ATP-PRTs) are believed to have different conformations. (rcsb.org)
  • The C. jejuni enzyme is similar to the previously described enzymes of the ATP-PRT long form, but exists only as hexameric species under experimental conditions, which contradicts previous assumptions that the hexamer is exclusively inactive. (canterbury.ac.nz)
  • This study aimed at evaluating the concentration of erythrocyte purine nucleotides (ATP, ADP, AMP, IMP) in trained and sedentary subjects before and after maximal physical exercise together with measuring the activity of purine metabolism enzymes as well as the concentration of purine (hypoxanthine, xanthine, uric acid) and pyrimidine (uridine) degradation products in blood. (springer.com)
  • For example, in vitro studies have shown that Ca 2+ flux through channels such as ryanodine receptors and IP 3 receptors mediate privileged communication between endoplasmic reticulum/sarcoplasmic reticulum (ER/SR) 3 and mitochondria and that this paces cellular metabolism by stimulating oxidative ATP production via interaction with TCA cycle enzymes ( 2 - 4 ). (pubmedcentralcanada.ca)
Pyrophosphate4
  • ATP reacts reversibly with 5-phosphoribosyl-1-pyrophosphate to yield N-1-(5'-phosphoribosyl)-ATP and pyrophosphate. (childrensmercy.org)
  • The resulting compound then reacts with an ATP phosphoribosyltransferase resulting in the release of pyrophosphate and 1-(5-phosphoribosyl)-ATP. (smpdb.ca)
  • Specifically, Phosphoribosyl-ATP is substrate for phosphoribosyl pyrophosphate synthetase 1. (ecmdb.ca)
  • The ribose-5-P is converted into phosphoribosyl pyrophosphate by Pyrophospho Kinase in this reaction ATP is consumed. (biochemden.com)
Mycobacterium Tuberculosis2
  • The crystal structures of the N-1-(5'-phosphoribosyl)-ATP transferase from Mycobacterium tuberculosis in complex with inhibitor histidine and AMP has been determined to 1.8 A resolution and without ligands to 2.7 A resolution. (tamu.edu)
  • Discovery of novel nitrobenzothiazole inhibitors for Mycobacterium tuberculosis ATP phosphoribosyl transferase (HisG) through virtual screening. (blogspot.com)
Hexameric1
  • ATP-phosphoribosyltransferase (ATP-PRT) is a hexameric enzyme in conformational equilibrium between an open and seemingly active state and a closed and presumably inhibited form. (rcsb.org)
Gene1
  • ATP-binding, Magnesium, Nucleotide-binding: Gene summary (Entrez) i. (youngtechies.com)
Allosteric5
  • Here, we develop a screening strategy for modulators of ATP-PRT and identify 3-(2-thienyl)-L-alanine (TIH) as an allosteric activator of this enzyme. (rcsb.org)
  • Here the authors show that in both states, ATP-PRT has a similar structural arrangement, suggesting that dynamic alterations are involved in ATP-PRT regulation by allosteric modulators. (rcsb.org)
  • Mechanism of feedback allosteric inhibition of ATP phosphoribosyltransferase. (rcsb.org)
  • In the long form ATP-PRT histidine binds to the allosteric site at the regulatory domain, but the exact nature of the inhibitory mechanism is still debated. (canterbury.ac.nz)
  • In summary this thesis supports the existence of a simple physical regulatorymechanism for the feedback inhibition of the ATP-PRT long form, the change between two different hexamer conformations depending on the presence of the allosteric effector. (canterbury.ac.nz)
Energetically expensive1
  • ATP-PRT also represents a metabolic control point, directing the flux of metabolites through this energetically expensive pathway. (canterbury.ac.nz)
Synthase1
  • In E. coli citrate synthase, the enzyme involved in the condensation reaction initiating the Citric Acid Cycle is strongly inhibited by α-ketoglutarate feedback inhibition and can be inhibited by DPNH as well high concentrations of ATP. (wikipedia.org)
Diphosphate1
  • The systematic name of this enzyme class is 1-(5-phospho-D-ribosyl)-ATP:diphosphate phospho-alpha-D-ribosyl-transferase. (wikipedia.org)
HGPRT1
  • The study demonstrated a significantly higher concentration of ATP in the erythrocytes of trained subjects which, in part, may be explained by higher metabolic activity on the purine re-synthesis pathway (significantly higher PRPP-S, APRT and HGPRT activities). (springer.com)
Hypoxanthine1
  • Free guanine is salvaged in the same way except it requires hypoxanthine-guanine phosphoribosyltransferase. (youngtechies.com)
Metabolism2
  • Phosphoribosyl-ATP takes part in the Histidine Metabolism pathway. (ecmdb.ca)
  • RYR2-mediated Ca 2+ fluxes are therefore proximal controllers of mitochondrial Ca 2+ , ATP levels, and a cascade of transcription factors controlling metabolism and survival. (pubmedcentralcanada.ca)
Catalyses1
  • On the other hand, adenosine kinase (EC 2.7.1.20) catalyses the salvage synthesis of AMP from adenosine and ATP. (springer.com)
Reaction6
  • This reaction requires aspartate, glutamine, bicarbonate, and 2 ATP molecules (to provide energy), as well as PRPP which provides the ribose-monophosphate. (youngtechies.com)
  • The reaction needs energy from ATP hydrolysis. (biochemden.com)
  • Here amide donor is Glutamine and it is ATP consumed reaction. (biochemden.com)
  • This carbon dioxide fixation reaction does not require biotin or ATP. (biochemden.com)
  • In this reaction, one Aspartic acid linked with Carboxyl group ATP is consumed. (biochemden.com)
  • b) The first step of the ATP-dependent aminoacylation reaction activates amino acid to generate an aminoacyl adenylate intermediate. (schoolbag.info)
Regulatory3
  • ATP phosphoribosyltransferase is found in two distinct forms: a long form containing two catalytic domains and a C-terminal regulatory domain, and a short form in which the regulatory domain is missing. (wikipedia.org)
  • Two distinctly different forms of ATP-PRT exist, the long form and the short form, which differ in the presence of a C-terminal regulatory domain. (canterbury.ac.nz)
  • The existence of a regulatory response to the level of histidine was further supported by the modified development of rosette leaves, flowers, and siliques in transgenic lines over-expressing ATP-PRT1 or ATP-PRT2 . (botanyconference.org)
Regulation1
  • ATP-PRT activity is modulated by two layers of regulation: active site inhibition by adenosine monophosphate, which reflects cellular energy levels, and pathway end product feedback inhibition by histidine. (canterbury.ac.nz)
Amino acid1
  • First, an amino acid is activated via ATP to form an aminoacyl adenylate intermediate. (schoolbag.info)
Oxidative1
  • Glycolysis requires relatively more NAD to generate ATP compared to the oxidative phosphorylation normally occurring in non-malignant tissues. (biomedcentral.com)
RCSB PDB - 5LHU: ATP Phosphoribosyltransferase from Mycobacterium tuberculosis in complex with the allosteric inhibitor L...
RCSB PDB - 5LHU: ATP Phosphoribosyltransferase from Mycobacterium tuberculosis in complex with the allosteric inhibitor L... (rcsb.org)
B2TYG1 | SWISS-MODEL Repository
B2TYG1 | SWISS-MODEL Repository (swissmodel.expasy.org)
IUCr) Acta Crystallographica Section D Volume 56, Part 11, November 2000
IUCr) Acta Crystallographica Section D Volume 56, Part 11, November 2000 (journals.iucr.org)
HisE - Phosphoribosyl-ATP pyrophosphatase - Gordonia sp. MMS17-SY073 - hisE gene & protein
HisE - Phosphoribosyl-ATP pyrophosphatase - Gordonia sp. MMS17-SY073 - hisE gene & protein (uniprot.org)
Engineering and application of a biosensor with focused ligand specificity | Nature Communications
Engineering and application of a biosensor with focused ligand specificity | Nature Communications (nature.com)
Geochemical Stability of Dissolved Mn(III) in the Presence of Pyrophosphate as a Model Ligand: Complexation and...
Geochemical Stability of Dissolved Mn(III) in the Presence of Pyrophosphate as a Model Ligand: Complexation and... (bioportfolio.com)
Nicotinamide Phosphoribosyltransferase Deficiency Potentiates the Antiproliferative Activity of Methotrexate through Enhanced...
Nicotinamide Phosphoribosyltransferase Deficiency Potentiates the Antiproliferative Activity of Methotrexate through Enhanced... (jpet.aspetjournals.org)
Site - hisG
Site - hisG (cgsc.biology.yale.edu)
Amino acid synthesis - Wikipedia
Amino acid synthesis - Wikipedia (en.wikipedia.org)
Figures and data in Comparative genomics explains the evolutionary success of reef-forming corals | eLife
Figures and data in Comparative genomics explains the evolutionary success of reef-forming corals | eLife (elifesciences.org)
Physiological and pathophysiological roles of NAMPT and NAD metabolism | Nature Reviews Endocrinology
Physiological and pathophysiological roles of NAMPT and NAD metabolism | Nature Reviews Endocrinology (nature.com)
Samantha Campbell Casey
Samantha Campbell Casey (napier.ac.uk)
Frontiers | NAD+ Metabolism as an Emerging Therapeutic Target for Cardiovascular Diseases Associated With Sudden Cardiac Death ...
Frontiers | NAD+ Metabolism as an Emerging Therapeutic Target for Cardiovascular Diseases Associated With Sudden Cardiac Death ... (frontiersin.org)
Computational Chemistry (computational physics) PhD Projects, Programs & Scholarships
Computational Chemistry (computational physics) PhD Projects, Programs & Scholarships (findaphd.com)
Publications - Dr Gerald Larrouy-Maumus
Publications - Dr Gerald Larrouy-Maumus (imperial.ac.uk)
COMPARISON OF PRIMARY HUMAN HEPATOCYTES AND HEPATOMA CELL LINE HEPG2 WITH REGARD TO THEIR BIOTRANSFORMATION PROPERTIES | Drug...
COMPARISON OF PRIMARY HUMAN HEPATOCYTES AND HEPATOMA CELL LINE HEPG2 WITH REGARD TO THEIR BIOTRANSFORMATION PROPERTIES | Drug... (dmd.aspetjournals.org)
Histidine - Wikipedia
Histidine - Wikipedia (en.wikipedia.org)
Essential Metabolites of Mycobacterium tuberculosis and Their Mimics | mBio
Essential Metabolites of Mycobacterium tuberculosis and Their Mimics | mBio (mbio.asm.org)
IL-10 Controls Early Microglial Phenotypes and Disease Onset in ALS Caused by Misfolded Superoxide Dismutase 1 | Journal of...
IL-10 Controls Early Microglial Phenotypes and Disease Onset in ALS Caused by Misfolded Superoxide Dismutase 1 | Journal of... (jneurosci.org)
High Throughput Crystallography of TB Drug Targets | Bentham Science
High Throughput Crystallography of TB Drug Targets | Bentham Science (eurekaselect.com)
Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like...
Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like... (epub.uni-regensburg.de)
Identification, cloning and characterization of sis7 and sis10 sugar-insensitive mutants of Arabidopsis | BMC Plant Biology |...
Identification, cloning and characterization of sis7 and sis10 sugar-insensitive mutants of Arabidopsis | BMC Plant Biology |... (bmcplantbiol.biomedcentral.com)
ATP Phosphoribosyltransferase | Profiles RNS
ATP Phosphoribosyltransferase | Profiles RNS (researchers.childrensmercy.org)
Shotgun metagenomics reveals an enrichment of potentially cross-reactive bacterial epitopes in ankylosing spondylitis patients,...
Shotgun metagenomics reveals an enrichment of potentially cross-reactive bacterial epitopes in ankylosing spondylitis patients,... (ard.bmj.com)
Modeling trophic dependencies and exchanges among insects' bacterial symbionts in a host-simulated environment | BMC Genomics |...
Modeling trophic dependencies and exchanges among insects' bacterial symbionts in a host-simulated environment | BMC Genomics |... (bmcgenomics.biomedcentral.com)
Genetic Dissection of Histidine Biosynthesis in Arabidopsis | Plant Physiology
Genetic Dissection of Histidine Biosynthesis in Arabidopsis | Plant Physiology (plantphysiol.org)
JCI - Volume 58, Issue 1
JCI - Volume 58, Issue 1 (jci.org)
Isotopic Steady-State Flux Analysis | Springer for Research & Development
Isotopic Steady-State Flux Analysis | Springer for Research & Development (rd.springer.com)
Comparison of human erythrocyte purine nucleotide metabolism and blood purine and pyrimidine degradation product concentrations...
Comparison of human erythrocyte purine nucleotide metabolism and blood purine and pyrimidine degradation product concentrations... (link.springer.com)