An enzyme that catalyzes the first step of the pathway for histidine biosynthesis in Salmonella typhimurium. ATP reacts reversibly with 5-phosphoribosyl-1-pyrophosphate to yield N-1-(5'-phosphoribosyl)-ATP and pyrophosphate. EC 2.4.2.17.
An enzyme that catalyzes the conversion of 5-phosphoribosyl-1-pyrophosphate and hypoxanthine, guanine, or 6-mercaptopurine to the corresponding 5'-mononucleotides and pyrophosphate. The enzyme is important in purine biosynthesis as well as central nervous system functions. Complete lack of enzyme activity is associated with the LESCH-NYHAN SYNDROME, while partial deficiency results in overproduction of uric acid. EC 2.4.2.8.
An enzyme catalyzing the formation of AMP from adenine and phosphoribosylpyrophosphate. It can act as a salvage enzyme for recycling of adenine into nucleic acids. EC 2.4.2.7.
Enzymes of the transferase class that catalyze the transfer of a pentose group from one compound to another.

An aminoacyl-tRNA synthetase paralog with a catalytic role in histidine biosynthesis. (1/30)

In addition to their essential catalytic role in protein biosynthesis, aminoacyl-tRNA synthetases participate in numerous other functions, including regulation of gene expression and amino acid biosynthesis via transamidation pathways. Herein, we describe a class of aminoacyl-tRNA synthetase-like (HisZ) proteins based on the catalytic core of the contemporary class II histidyl-tRNA synthetase whose members lack aminoacylation activity but are instead essential components of the first enzyme in histidine biosynthesis ATP phosphoribosyltransferase (HisG). Prediction of the function of HisZ in Lactococcus lactis was assisted by comparative genomics, a technique that revealed a link between the presence or the absence of HisZ and a systematic variation in the length of the HisG polypeptide. HisZ is required for histidine prototrophy, and three other lines of evidence support the direct involvement of HisZ in the transferase function. (i) Genetic experiments demonstrate that complementation of an in-frame deletion of HisG from Escherichia coli (which does not possess HisZ) requires both HisG and HisZ from L. lactis. (ii) Coelution of HisG and HisZ during affinity chromatography provides evidence of direct physical interaction. (iii) Both HisG and HisZ are required for catalysis of the ATP phosphoribosyltransferase reaction. This observation of a common protein domain linking amino acid biosynthesis and protein synthesis implies an early connection between the biosynthesis of amino acids and proteins.  (+info)

Specific binding of the first enzyme for histidine biosynthesis to the DNA of histidine operon. (2/30)

Studies were done to examine direct binding of the first enzyme of the histidine biosynthetic pathway (phosphoribosyltransferase) to 32P-labeled phi80dhis DNA and competition of this binding by unlabeled homologous DNA and by various preparations of unlabeled heterologous DNA, including that from a defective phi80 bacteriophage carrying the histidine operon with a deletion of part of its operator region. Our findings show that phosphoribosyltransferase binds specifically to site in or near the regulatory region of the histidine operon. The stability of the complex formed by interaction of the enzyme with the DNA was markedly decreased by the substrates of the enzyme and was slightly increased by the allosteric inhibitor, histidine. These findings are consistent with previous data that indicate that phosphoribosyltransferase plays a role in regulating expression of the histidine operon.  (+info)

Molecular dissection of the role of histidine in nickel hyperaccumulation in Thlaspi goesingense (Halacsy). (3/30)

To understand the role of free histidine (His) in Ni hyperaccumulation in Thlaspi goesingense, we investigated the regulation of His biosynthesis at both the molecular and biochemical levels. Three T. goesingense cDNAs encoding the following His biosynthetic enzymes, ATP phosphoribosyltransferase (THG1, GenBank accession no. AF003347), imidazoleglycerol phosphate dehydratase (THB1, GenBank accession no. AF023140), and histidinol dehydrogenase (THD1, GenBank accession no. AF023141) were isolated by functional complementation of Escherichia coli His auxotrophs. Northern analysis of THG1, THD1, and THB1 gene expression revealed that each gene is expressed in both roots and shoots, but at the concentrations and dosage times of Ni treatment used in this study, these genes failed to show any regulation by Ni. We were also unable to observe any increases in the concentration of free His in root, shoot, or xylem sap of T. goesingense in response to Ni exposure. X-ray absorption spectroscopy of root and shoot tissue from T. goesingense and the non-accumulator species Thlaspi arvense revealed no major differences in the coordination of Ni by His in these tissues. We therefore conclude that the Ni hyperaccumulation phenotype in T. goesingense is not determined by the overproduction of His in response to Ni.  (+info)

Molecular cloning and characterization of ATP-phosphoribosyl transferase from Arabidopsis, a key enzyme in the histidine biosynthetic pathway. (4/30)

We have characterized two isoforms of ATP-phosphoribosyl transferase (ATP-PRT) from Arabidopsis (AtATP-PRT1 [accession no. AB025251] and AtATP-PRT2), catalyzing the first step of the pathway of hisidine (His) biosynthesis. The primary structures deduced from AtATP-PRT1 and AtATP-PRT2 cDNAs share an overall amino acid identity of 74.6% and contain N-terminal chloroplast transit peptide sequences. DNA-blot analyses indicated that the ATP-PRTs in Arabidopsis are encoded by two separate genes with a closely similar gene structural organization. Both gene transcripts were detected throughout development, and protein-blot analysis revealed predominant accumulation of the AtATP-PRT proteins in Arabidopsis leaves. The His auxotrophy of a his1 mutant of Saccharomyces cerevisiae was suppressed by the transformation with AtATP-PRT1 and AtATP-PRT2 cDNAs, indicating that both isoforms are functionally active ATP-PRT enzymes. The K(m) values for ATP and phosphoribosyl pyrophosphate of the recombinant AtATP-PRT proteins were comparable to those of the native ATP-PRTs from higher plants and bacteria. It was demonstrated that the recombinant AtATP-PRTs were inhibited by L-His (50% inhibition of initial activity = 40-320 microM), suggesting that His biosynthesis was regulated in plants through feedback inhibition by L-His.  (+info)

Evidence against a covalent intermediate in the adenosine triphosphate phosphoribosyltransferase reaction of histidine biosynthesis. (5/30)

14C-Labeled 5-phospho-alpha-D-ribose-1-diphosphate (PRibPP) was synthesized and its interaction with adenosine triphosphate phosphoribosyltransferase was examined by gel filtration in a search for a form of this substrate covalently bound to the enzyme. Wide variation in solvent conditions gave little labeling of the enzyme. Heavy labeling was found only in the presence of the second substrate, ATP, and this was shown to arise from tightly but noncovalently bound product. Previous reports of a covalent intermediate in this enzymatic reaction probably were due to contaminating ATP in 5-phospho-alpha-D-ribose-1-diphosphate. Feedback inhibition of the enzyme by histidine was shown to occur at the step giving product or at some earlier step in the mechanism.  (+info)

trans-Recessive mutation in the first structural gene of the histidine operon that results in constitutive expression of the operon. (6/30)

The first enzyme for histidine biosynthesis, encoded in the hisG gene, is involved in regulation of expression of the histidine operon in Salmonella typhimurium. The studies reported here concern the question of how expression of the histidine operon is affected by a mutation in the hisG gene that alters the allosteric site of the first enzyme for histidine biosynthesis, rendering the enzyme completely resistant to inhibition by histidine. The intracellular concentrations of the enzymes encoded in the histidine operon in a strain carrying such a mutation on an episome and missing the chromosomal hisG gene are three- to fourfold higher than in a strain carrying a wild-type hisG gene on the episome. The histidine operon on such a strain fails to derepress in response to histidine limitation and fails to repress in response to excess histidine. Furthermore, utilizing other merodiploid strains, we demonstrate that the wild-type hisG gene is trans dominant to the mutant allele with respect to this regulatory phenomenon. Examination of the regulation of the histidine operon in strains carrying the feedback-resistant mutation in an episome and hisT and hisW mutations in the chromosome showed that the hisG regulatory mutation is epistatic to the hisT and hisW mutations. These data provide additional evidence that the first enzyme for histidine biosynthesis is involved in autogenous regulation of expression of the histidine operon.  (+info)

Derepression and repression of the histidine operon: role of the feedback site of the first enzyme. (7/30)

Thiazolealanine, a false feedback inhibitor, causes transient repression of the his operon previously derepressed by a severe histidine limitation in strains with a wild-type or feedback-hypersensitive first enzyme but not in feedback-resistant mutants. Since experiments reported here clearly demonstrate that thiazolealanine is not transferred to tRNAHis, it is proposed that this "transient repression" is effected through the interaction of thiazolealanine with the feedback site of the enzyme. Experiments in the presence of rifampin indicate that this thiazolealanine-mediated effect is exerted at the level of translation. We conclude that histidine (free), in addition to forming co-repressor, also represses the operon at the level of translation through feedback interaction with the first enzyme of the pathway (adenosine 5'-triphosphate phosphoribosyltransferase). Rates of derepression in feedback-resistant strains are roughly half of those observed in controls, suggesting a positive role played by a first enzyme with a normal but unoccupied feedback site. Some feedback-resistant mutants, in contrast to the wild type, were unable to exhibit derepression under histidine limitation caused by aminotriazole.  (+info)

Affinity labelling to - SH groups in adenosine - triphosphate - phosphoribosyl transferase with the dinitrophenyl group from S-dinitrophenyl-6-mercaptopurine-riboside 5'-phosphate. (8/30)

Adenosine-triphosphate-phosphoribosyl transferase from Escherichia coli reacts with S-dinitrophenyl-6-mercaptopurine-riboside 5'-phosphate. In this reaction the dinitrophenyl group becomes attached to the enzyme, while the nucleotide is split off. Most aliphatic high and low-molecular-weight-SH compounds react with the thioether in the opposite way, i.e. bind the nucleotide and split off dinitrothiophenol. It appears that the dinitrophenyl moiety of the thioether interacts with the enzyme in a specific way, and that this interaction activates the bond between the dinitrophenyl group and the sulfur atom. In support of this it was found that dinitrophenol inhibits the transferase reaction with half maximal effect at 0.4 mM. The inhibition is competitive with ATP. Dinitrophenol also competes with ATP in binding studies.  (+info)

Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N-(5-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity (By similarity).
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Abstract: Allosteric modulation of catalysis is a common regulatory strategy of flux-controlling biosynthetic enzymes. The enzyme ATP phosphoribosyltransferase (ATPPRT) catalyses the first reaction in histidine biosynthesis, the magnesium-dependent condensation of ATP and 5-phospho--D-ribosyl-1-pyrophosphate (PRPP) to generate N1-(5-phospho--D-ribosyl)-ATP (PRATP) and pyrophosphate (PPi). ATPPRT is allosterically inhibited by the final product of the pathway, histidine. Hetero-octameric ATPPRT consists of four catalytic subunits (HisGS) and four regulatory subunits (HisZ) engaged in intricate catalytic regulation. HisZ enhances HisGS catalysis in the absence of histidine while mediating allosteric inhibition in its presence. Here we report HisGS structures for the apoenzyme and complexes with substrates (PRPP, PRPP-ATP, PRPP-ADP), product (PRATP), and inhibitor (AMP), along with ATPPRT holoenzyme structures in complexes with substrates (PRPP, PRPP-ATP, PRPP-ADP) and product (PRATP). These ...
Analysis of the redundant first step in His biosynthesis catalyzed by ATP phosphoribosyl transferase required the construction of plants heterozygous for knockout alleles of both HISN1A (At1g58080) and HISN1B (At1g09795). The mutant alleles examined contained insertions near the beginning (hisn1b-1) or middle (hisn1a-1) of the coding region. Double knockouts for other redundant HISN genes could not be constructed because confirmed insertions remain to be identified for HISN5B and HISN6B. Twenty progeny plants from a selfed double heterozygote (AaBb) segregating for both hisn1a-1 (a) and hisn1b-1 (b) were PCR genotyped and screened for defects in seed and ovule development. The following plants were identified: AABb (5), AAbb (2), AaBB (5), and AaBb (8). Despite the small sample size, these results are consistent with two important conclusions supported by further studies: reduced transmission of double knockout gametes (ab) and embryo lethality of double homozygotes (aabb). No viable double ...
Ribbon representation of the structure of an enzyme known as ATP-PRT from TB bacteria (blue), bound to an allosteric activator (pink).
Mitotic spindles are primarily composed of microtubules (MTs), generated by polymerization of α- and β-Tubulin hetero-dimers [1, 2]. Tubulins undergo a series of protein folding and post-translational modifications in order to fulfill their functions [3, 4]. Defects in Tubulin polymerization dramatically affect spindle formation and disrupt chromosome segregation. We recently described a role for the product of the conserved misato (mst) gene in regulating mitotic MT generation in flies [5], but the molecular function of Mst remains unknown. Here, we use affinity purification mass spectrometry (AP-MS) to identify interacting partners of Mst in the Drosophila embryo. We demonstrate that Mst associates stoichiometrically with the hetero-octameric Tubulin Chaperone Protein-1 (TCP-1) complex, with the hetero-hexameric Tubulin Prefoldin complex, and with proteins having conserved roles in generating MT-competent Tubulin. We show that RNAi-mediated invivo depletion of any TCP-1 subunit phenocopies ...
In polarized epithelial cells, vectorial protein trafficking is important for transporting specific membrane proteins to generate distinct apical and basolateral membrane protein compositions. The Exocyst is a conserved hetero-octameric protein complex, which regulates different aspects of protein trafficking, including tethering of the Golgi-derived vesicles to target membranes. Two of the Exocyst subunits, Sec5 and Exo84, competitively bind to the small GTPases, RalA and RalB, in a GTP-dependent manner. Although Ral GTPases have been proposed to mediate assembly of Exocyst holocomplexes, we hypothesize that they actually serve to allosterically regulate Exocyst functions by promoting association or disassociation of additional factors. Previous studies have shown that active RalA, but not RalB, accelerated basolateral exocytosis of E-cadherin. In contrast, knockdown of RalB, but not RalA, disrupts endocytosis of E-cadherin. However, mechanisms by which association of Ral GTPases with Sec5 and Exo84
Metabolism. Shui, Wang [1], Guirong, Zhang [2], Wang, Hongyun [3], Ulanov, Alexander [3], Lozovaya, Vera [3], Lin, Yun [4]. A regulatory role of histidine in Arabidopsis metabolism and growth revealed by the low oil 1 mutant that corresponds to ATP-Phosphoribosyl Transferase 1, a key enzyme of histidine biosynthesis.. Amino acid sensory mechanisms are known to coordinate metabolism and growth in microbes and animals. However, evidence for their existence in plants has been elusive. In a genetic screen for Arabidopsis seed oil and protein deposition mutants, the low oil 1 (loo1) was isolated and map-based gene cloning identified a missense mutation in the first histidine biosynthetic enzyme ATP-phosphoribosyl transferase 1 (ATP-PRT1). The loo1 mutant exhibited development and growth defects throughout the entire lifespan. The severely retarded root elongation of loo1 seedlings was rescued by supplemental histidine in the growth medium. Transcript and metabolite profiling and real-time RT-PCR ...
TY - JOUR. T1 - Identification of novel bacterial histidine biosynthesis inhibitors using docking, ensemble rescoring, and whole-cell assays. AU - Henriksen,Signe Teuber. AU - Liu,J.. AU - Estiu,G.. AU - Oltvai,Z.N.. AU - Wiest,O.. PY - 2010. Y1 - 2010. N2 - The rapid spread on multidrug-resistant strains of Staphylococcus aureus requires not just novel treatment options, but the development of faster methods for the identification of new hits for drug development. The exponentially increasing speed of computational methods makes a more extensive use in the early stages of drug discovery attractive if sufficient accuracy can be achieved. Computational target identification using systems-level methods suggested the histidine biosynthesis pathway as an attractive target against S. aureus. Potential inhibitors for the pathway were identified through docking, followed by ensemble rescoring, that is sufficiently accurate to justify immediate testing of the identified compounds by whole-cell assays, ...
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via ...
Displaying operon conservation. Part A shows that the order of genes belonging to the histidine operon is well conserved in this set of proteobacterial genomes.
This clade of sequences is highly similar to the HisF protein, but generally represents the second HisF homolog in the genome where the other is an authentic HisF observed in the context of a complete histidine biosynthesis operon. The similarity between these WbuZ sequences and true HisFs is such that often the closest match by BLAST of a WbuZ is a HisF. Only by making a multiple sequence alignment is the homology relationship among the WbuZ sequences made apparent. WbuZ genes are invariably observed in the presence of a homolog of the HisH protein (designated WbuY) and a proposed N-acetyl sugar amidotransferase designated in WbuX in E. coli [1], IfnA in P. aeriginosa [2] and PseA in C. jejuni [3]. Similarly, this trio of genes is invariably found in the context of saccharide biosynthesis loci. It has been shown that the WbuYZ homologs are not essential components of the activity expressed by WbuX, leading to the proposal that these to proteins provide ammonium ions to the amidotransferase when ...
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
Hello, This question might seem easy, but I found it not so obvious as I thought about it more: What does rate limiting mean to you ? It is used quite often, and not only in biology. The example I am dealing with is a common one, a metabolic pathway. The simplest way to see it, is that there is not RenoughS of the first enzyme on the pathway and RplentyS of the following enzymes. When more of the first enzyme is made, the flux through the pathway increases in a direct (not necessarily linear) function of this first enzyme. Does it mean that this first enzyme is used at saturation of its substrate(s) ? If not, the element that keeps those substrate(s) constant would be the real rate limiting step by providing the right flux of substrate so as to keep it constant ! If that flux does not change, an increase in the quantity and consequently in flux of the first enzyme would have the effect of lowering the concentration of its substrate until the activity was restored to its original value ! I feel ...
Steroidal glycoalkaloids (SGAs) are plant secondary metabolites known to be toxic to animals and humans and that have putative roles in defense against pests. The proposed mechanisms of SGA toxicity are sterol-mediated disruption of membranes and inhibition of cholinesterase activity in neurons. It has been suggested that phytopathogenic microorganisms can overcome SGA toxicity by enzymatic deglycosylation of SGAs. Here, we have explored SGA-mediated toxicity toward the invasive oomycete Phytophthora infestans, the causative agent of the late blight disease in potato and tomato, as well as the potential for SGA deglycosylation by this species. Our growth studies indicate that solanidine, the nonglycosylated precursor of the potato SGAs a-chaconine and a-solanine, has a greater physiological impact than its glycosylated forms. All of these compounds were incorporated into the mycelium, but only solanidine could strongly inhibit the growth of P. infestans in liquid culture. Genes encoding several ...
Vega doubled to right center and crossed the plate when senior Marc Vega reached base on an error.. Aguilar went one inning and fanned one with an unearned run, junior Xavier Dubon tossed a perfect fifth inning and senior Jacob Real toured a hitless seventh inning, walking one and striking out one.. In three of the seven frames, the Indians (4-5) were retired in order by Turner.. Turner delivered six and one-third effective innings, allowing two hits, walking four with three strikeouts and two hit batters.. In the first inning, after sophomore Brian Garcia bounced back to Turner, senior Revin Diego lashed a single up the middle and moved to second base on a wild pitch.. That would be it for Diego as senior Nathan Palafox then lined out and Barrera grounded out.. In the third inning, the Indians were able to muster a one-out walk by junior designated hitter Collin Johnson.. Johnson was erased when Garcia reached on a force out and Garcia was erased on Diegos force out.. In the fifth inning, ...
http://sanggahtoksago.blogspot.com/ Penarik Beca menulis agak menusuk juga dalam postingnya. Mengulas tentang kekalahan PAS di galas || Dalam postingnya bertajuk Setelah Galas, jangan malas, Dato Mohd Ariff Sabri Abdul Aziz menyatakan: Maka persoalan yang harus di pertimbangkan oleh Mustapha ialah adakah puak Umno sanggup mengulangi cara kempen di Galas? Apakah cara kempennya? Cara yang lebih subdued dan…
The Tang, Garg, and Houk research groups have discovered natures natural protein catalysts (enzymes) that catalyze the Alder-ene reaction.
Researchers at UCLA report that engaging in the practice of mindfulness meditation stopped the decline of CD4 T immune cells in stressed HIV-positive patients, thus slowing the progression of the disease.
Contrasting effects of nicotianamine synthase knockdown on zinc and nickel tolerance and accumulation in the zinc/cadmium hyperaccumulator Arabidopsis halleri ...
Histidine biosynthesis bifunctional protein HisIE; Protein involved in phosphoribosyl-AMP cyclohydrolase activity, phosphoribosyl-ATP diphosphatase activity and histidine biosynthetic process; In the N-terminal section; belongs to the PRA-CH family (213 aa ...
now, downstream data, issues, survivors, sites, data, and univariate representative RNAs could require purified via the ebook of outcomes. The ebook to enhance acid promoters with production is that the ECD-mTLR2 cell is full to introduce possible, there misconfigured to subscribe. respectively, ebook Investitionen, has acidic certain attB, and the network phosphoribosyltransferase is to be observed for a specifically new volume.
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Misture todos os ingredientes da polenta, menos o gorgonzola, numa panela funda e mexa sem parar. Tempere com sal à gosto e coloque um fio de azeite para não grudar. Quando começar a engrossar mexa rapidamente e proteja com a tampa da panela, pois espira muito. Deixe ficar mais cremosa e mais mole. Se ela endurecer, coloque um pouco mais de leite e mexa. Distribua em potinhos pequenos, regue com azeite e finalize com pedacinhos de gorgonzola. Servir quentinha é gostosa, mas se de repente esfriar, não se preocupe, pois não ficará ruim ...
... by the enzyme ATP-phosphoribosyl transferase. ATP-phosphoribosyl transferase is indicated by His1 in the image. His4 gene ... ATP-phosphoribosyl transferase (shown as His1 in the image on the right). ATP-phosphoribosyl transferase is the rate ... This pathway requires energy in order to occur therefore, the presence of ATP activates the first enzyme of the pathway, ... The first reaction of histidine biosynthesis is the condensation of PRPP and adenosine triphosphate (ATP) ...
... catalyzed by ATP-phosphoribosyl transferase. Phosphoribosyl-ATP converts to phosphoribosyl-AMP (PRAMP). His4 then catalyzes the ... In the asparagine synthetase reaction, ATP is used to activate aspartate, forming β-aspartyl-AMP. Glutamine donates an ammonium ... The enzyme asparagine synthetase produces asparagine, AMP, glutamate, and pyrophosphate from aspartate, glutamine, and ATP. ... is strongly inhibited by α-ketoglutarate feedback inhibition and can be inhibited by DPNH as well high concentrations of ATP. ...
The step of histidine biosynthesis is the condensation of ATP and PRPP by ATP-phosphoribosyl transferase, the rate determining ... R5P and its derivatives serve as precursors to many biomolecules, including DNA, RNA, ATP, coenzyme A, FAD (Flavin adenine ... The build up is caused by a deficiency of the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT), which leads to ... During the purine salvage pathway, phosphoribosyltransferases add PRPP to bases. PRPP also plays an important role in ...
ATP phosphoribosyltransferase regulatory subunit, LTA synthase family protein, type I DNA topoisomerase, nicotinate ... phosphoribosyltransferase, bifunctional hydroxymethylpyrimidine kinase/phospho- methylpyrimidine kinase, single-stranded-DNA- ...
... phosphoribosyl ATP synthetase, phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase, phosphoribosyl-ATP:pyrophosphate- ... an ATP phosphoribosyltransferase (EC 2.4.2.17) is an enzyme that catalyzes the chemical reaction 1-(5-phospho-D-ribosyl)-ATP + ... ATP phosphoribosyltransferase is found in two distinct forms: a long form containing two catalytic domains and a C-terminal ... Histidine biosynthesis is an energetically expensive process and ATP phosphoribosyltransferase activity is subject to control ...
... anthranilate phosphoribosyltransferase MeSH D08.811.913.400.725.200 - ATP phosphoribosyltransferase MeSH D08.811.913.400. ... 725.450 - hypoxanthine phosphoribosyltransferase MeSH D08.811.913.400.725.700 - orotate phosphoribosyltransferase MeSH D08.811. ... atp citrate (pro-s)-lyase MeSH D08.811.913.050.350 - carnitine acyltransferases MeSH D08.811.913.050.350.170 - carnitine O- ... atp-dependent proteases MeSH D08.811.277.656.149.200 - endopeptidase clp MeSH D08.811.277.656.149.500 - protease la MeSH ...
... trafficking GUK1 Guanylate kinase transfers phosphate from ATP to GMP HPRT Hypoxanthine-guanine phosphoribosyltransferase ... 001689 Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c ATP5H NM_006356 Homo sapiens ATP ... ATP reservoir) Cytidine deaminase questionable: not present in very high levels at all CPNE1 ENSA (gene) FTH1 Heavy chain of ... 004046 Homo sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, alpha ATP5B NM_001686 ATP5C1 NM_005174 ATP5D NM_ ...
Uridylate (UMP) is later converted to UDP via phosphorylation by UMP kinase and ATP and then nucleoside diphosphate kinase ... Like other pyrimidine phosphoribosyltransferases, orotate phosphoribosyltransferase has a flexible loop that moves to position ... Orotate phosphoribosyltransferase (OPRTase) or orotic acid phosphoribosyltransferase is an enzyme involved in pyrimidine ... Pyrimidine phosphoribosyltransferases such as orotate phosphoribosyltransferase activate their substrates by forming SN1-like ...
iNAMPT can also catalyze the synthesis of NMN from phosphoribosyl pyrophosphate (PRPP) when ATP is present. eNAMPT has been ... Nicotinamide phosphoribosyltransferase (NAmPRTase or NAMPT), formerly known as pre-B-cell colony-enhancing factor 1 (PBEF1) or ... Grolla AA, Travelli C, Genazzani AA, Sethi JK (July 2016). "Extracellular nicotinamide phosphoribosyltransferase, a new cancer ... September 2006). "Crystal structure of visfatin/pre-B cell colony-enhancing factor 1/nicotinamide phosphoribosyltransferase, ...
ATP phosphoribosyltransferase EC 2.4.2.18: anthranilate phosphoribosyltransferase EC 2.4.2.19: nicotinate-nucleotide ... uracil phosphoribosyltransferase EC 2.4.2.10: orotate phosphoribosyltransferase EC 2.4.2.11: now EC 6.3.4.21 nicotinate ... nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase EC 2.4.2.22: xanthine phosphoribosyltransferase EC 2.4. ... adenine phosphoribosyltransferase EC 2.4.2.8: hypoxanthine phosphoribosyltransferase EC 2.4.2.9: ...
ATP, and H2O, whereas its four products are nicotinate D-ribonucleotide, diphosphate, ADP, and phosphate. This enzyme belongs ... In enzymology, a nicotinate phosphoribosyltransferase (EC 6.3.4.21) is an enzyme that catalyzes the chemical reaction ... Kosaka A, Spivey HO, Gholson RK (1971). "Nicotinate phosphoribosyltransferase of yeast. Purification and properties". J. Biol. ... nicotinate + 5-phospho-α-D-ribose 1-diphosphate + ATP + H2O ⇌ {\displaystyle \rightleftharpoons } nicotinate D-ribonucleotide ...
The synthesis of IMP, (precursor to GMP and GTP, and to AMP and ATP) also requires THF, and also can be bypassed. In this case ... hypoxanthine-guanine phosphoribosyltransferase (HGPRT) reacts hypoxanthine absorbed from the medium with PRPP, liberating ...
ATP-dependent helicase, tRNA (cytidine(34)-2′-O)-methyltransferase, glutamine-fructose-6-phosphate transaminase (isomerizing), ... uracil phosphoribosyltransferase, L-histidine N(alpha)-methyltransferase, DUF58 domain-containing protein, NADH-quinone ...
The histidine biosynthesis pathway involves the reaction between PRPP and ATP, which activates the latter to ring cleavage. ... Decreased levels of hypoxanthine guanine phosphoribosyl transferase (HGPRT) causes this accumulation, as PRPP is a substrate ... Salmonella typhimurium nicotinate phosphoribosyltransferase". Journal of Biological Chemistry. 268 (34): 26004-26010. doi: ... with the first step being N-alkylation of anthranilic acid catalysed by the enzyme anthranilate phosphoribosyltransferase. ...
ATP stimulates production of GTP, while GTP stimulates production of ATP. This cross regulation keeps the relative amounts of ... Lesch-Nyhan syndrome is caused by a deficiency in hypoxanthine-guanine phosphoribosyltransferase or HGPRT, the enzyme that ... ATP, a purine nucleotide, is an activator of pyrimidine synthesis, while CTP, a pyrimidine nucleotide, is an inhibitor of ... XMP is then converted into GMP by using the hydrolysis of 1 ATP and the conversion of glutamine to glutamate. AMP and GMP can ...
Both steps are fueled by ATP hydrolysis: ATP + UMP → ADP + UDP UDP + ATP → UTP + ADP CTP is subsequently formed by the ... Orotate phosphoribosyltransferase (PRPP transferase) catalyzes the net reaction yielding orotidine monophosphate (OMP): Orotate ... Glutamine is the NH3 donor and the reaction is fueled by ATP hydrolysis, too: UTP + Glutamine + ATP + H2O → CTP + ADP + Pi ... to PRPP by reacting it with ATP. The reaction is unusual in that a pyrophosphoryl group is directly transferred from ATP to C1 ...
Cancer cells are known to become resistant to DAP by losing their adenine phosphoribosyltransferase (APRT) function, a process ... ATP + dGMP + L-aspartate = (d)ADP + phosphate + 2-aminodeoxyadenylosuccinate (dSMP) The resulting dSMP is processed by host ... 6-diaminopurine and 6-methylpurine that affect adenine phosphoribosyltransferase in Escherichia coli K-12]". Genetika. 13 (10 ...
Importantly, the process in the organelle has no net ATP synthesis. This ATP comes later from processes outside of the ... These enzymes found in the glycosome to help with synthesis are guanine and adenine phosphoribosyl transferase, hypoxanthine, ... This energy metabolism generates ATP through the process of glycolysis. The glycosome is a host of the main glycolytic enzymes ... the glycosomes attached to the myofibrils seem to serve the myosin by providing energy substrates for generation of ATP through ...
Conversely, PRPP and ATP act as positive effectors that enhance the enzyme's activity. Modulating the pyrimidine metabolism ... UMPS is a bifunctional enzyme consisting of orotate phosphoribosyltransferase (OPRT) and orotidine monophosphate decarboxylase ...
ATP) EC 6.5.1.2: DNA ligase (NAD+) EC 6.5.1.3: RNA ligase (ATP) EC 6.5.1.4: RNA 3′-terminal-phosphate cyclase (ATP) EC 6.5.1.5 ... nicotinate phosphoribosyltransferase EC 6.3.4.22: tRNAIle2-agmatinylcytidine synthase * EC 6.3.4.23: formate- ... RNA 3′-terminal-phosphate cyclase (GTP) * EC 6.5.1.6: DNA ligase (ATP or NAD+) * EC 6.5.1.7: DNA ligase (ATP, ADP or GTP) * EC ... As ATP is not hydrolysed during the reaction, the classification of the enzyme as a ligase was incorrect EC 6.3.5.9: ...
EC 6.5.1.1 ADN ligase (ATP) EC 6.5.1.2 ADN ligase (NAD+) EC 6.5.1.3 ARN ligase (ATP) EC 6.5.1.4 ARN 3'-terminal-phosphate ... tRNAIle-lysidine synthetase EC 6.3.4.20 7-cyano-7-deazaguanine synthase EC 6.3.4.21 nicotinate phosphoribosyltransferase EC 6.3 ... ATP, la GTP, la CTP, la TTP et l'UTP) pour catalyser sa réaction. En fait la synthase forme et défait les doubles liaisons ... ATP ou d'autres molécules similaires. Elle forme des liaisons phosphodiesters de l'extrémité 3' hydroxylée à l'extrémité 5' ...
This contrasts with eukaryotic DNA ligases, which use ATP to form the DNA-AMP intermediate. Li et al. have found that NAD+ ... The first step, and the rate-limiting enzyme in the salvage pathway is nicotinamide phosphoribosyltransferase (NAMPT), which ... Yamboliev IA, Smyth LM, Durnin L, Dai Y, Mutafova-Yambolieva VN (2009). "Storage and secretion of beta-NAD, ATP and dopamine in ... In most organisms, this enzyme uses ATP as the source of the phosphate group, although several bacteria such as Mycobacterium ...
fGAR + L-Glutamine + ATP → fGAM + L-Glutamate + ADP + Pi The fifth is catalyzed by AIR synthetase (FGAM cyclase). fGAM + ATP → ... The enzyme adenine phosphoribosyltransferase (APRT) salvages adenine. The enzyme hypoxanthine-guanine phosphoribosyltransferase ... CAIR + L-Aspartate + ATP → SAICAR + ADP + Pi The eight is catalyzed by adenylosuccinate lyase. SAICAR → AICAR + Fumarate The ... PRPP + L-Glutamine + H2O → PRA + L-Glutamate + PPi In the second step react PRA, glycine and ATP to create GAR, ADP, and ...
The first step for joining an amino acid to its corresponding tRNA is the formation of aminoacyl-AMP: Amino acid + ATP ↽ − − ⇀ ... It is caused by the absence of hypoxanthine-guanine phosphoribosyltransferase, which is a necessary enzyme for purine ... The following step requires the activation of glycine by the addition of a phosphate group from ATP. GAR synthetase performs ... High energy molecules, such as ATP, have three phosphates. Often, the terminal phosphate is split off during hydrolysis and ...
ATP synthase (EC 3.6.3.14) Kynureninase EC 3.7.1.3 EC 3.8.1.3 Haloacetate dehalogenase EC 3.9.1.1: Phosphoamidase EC 3.9.1.2: ... EC 2.4.2 Hypoxanthine-guanine phosphoribosyltransferase EC 2.4.2.8 Category:EC 2.5 Category:EC 2.5.1 Thiaminase EC 2.5.1.2 ... ATP-hydrolysing) Serine racemase Category:EC 5.1.2 Mandelate racemase Category:EC 5.1.3 UDP-glucose 4-epimerase Category:EC 5.1 ...
Functional Consequences of ATP- and dATP-Induced Oligomerization of the Large Subunit†". Biochemistry. 41 (2): 462-74. doi: ... "Different Oligomeric States are Involved in the Allosteric Behavior of Uracil Phosphoribosyltransferase from Escherichia Coli ... W in Complex with Nonhydrolyzable ATP Analogues Reveal a Putative Active Conformation of the Enzyme as a Result of Domain ... Dependent Malic Enzyme by ATP and Fumarate". Structure. 10 (7): 951-60. doi:10.1016/S0969-2126(02)00788-8. PMID 12121650. ...
Alternatively to ATP, GTP has been shown to act comparably as a phosphate donor. This promiscuity enables the important role ... "Epstein-Barr virus encoded nuclear protein EBNA-3 binds a novel human uridine kinase/uracil phosphoribosyltransferase". BMC ... hydroxyl group on the substrate and activates it to attack the γ-phosphorus of ATP. Structural analyses have shown that the ...
8] which results in accelerated hepatic breakdown of ATP and the generation of organic acids that compete with urate for ... Hypoxanthine guanine phosphoribosyltransferase (HGPRT) deficiency (Lesch-Nyhan syndrome): This is an inherited X-linked ... These include a complete deficiency of hypoxanthine guanine phosphoribosyltransferase (HGPRT) as in Lesch-Nyhan syndrome, ...
ABCG8: ATP binding cassette subfamily G member 8. *ABHD5: abhydrolase domain containing 5, lysophosphatidic acid ... APRT: adenine phosphoribosyltransferase. *APTX: aprataxin. *AQP2: aquaporin 2. *AR: androgen receptor. *ARFGEF2: ADP ... ABCG5: ATP binding cassette subfamily G member 5. * ... ABCA1: ATP binding cassette subfamily A member 1. *ABCA3: ATP ... ABCG2: ATP binding cassette subfamily G member 2 (Junior blood group). * ...
8] which results in accelerated hepatic breakdown of ATP and the generation of organic acids that compete with urate for ... Hypoxanthine guanine phosphoribosyltransferase (HGPRT) deficiency (Lesch-Nyhan syndrome): This is an inherited X-linked ... These include a complete deficiency of hypoxanthine guanine phosphoribosyltransferase (HGPRT) as in Lesch-Nyhan syndrome, ...
... phosphoribosyl ATP synthetase, phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase, phosphoribosyl-ATP:pyrophosphate- ... an ATP phosphoribosyltransferase (EC 2.4.2.17) is an enzyme that catalyzes the chemical reaction 1-(5-phospho-D-ribosyl)-ATP + ... ATP phosphoribosyltransferase is found in two distinct forms: a long form containing two catalytic domains and a C-terminal ... Histidine biosynthesis is an energetically expensive process and ATP phosphoribosyltransferase activity is subject to control ...
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate <=> 5-phospho-alpha-D-ribose 1-diphosphate + ATP. ...
ATP phosphoribosyltransferase; ATP phosphoribosyltransferase. - 195.45 1.42E-61 13 - 197 superfamily 246914 ... ATP phosphoribosyltransferase, C-terminal domain; This domain corresponds to the C-terminal third... cl06867 93.8063 5.10E-24 ... ATP phosphoribosyltransferase; Members of this family from B. subtilis, Aquifex aeolicus, and... cl15266 195.45 1.42E-61 13 - ... ATP phosphoribosyltransferase; Reviewed - 303.942 1.70E-102 13 - 290 multi-dom 234781 ...
ATP phosphoribosyltransferase UniProtKBInterProInteractive Modelling. 284 aa; Sequence (Fasta) ; 9 identical sequences: ...
Crystals of ATP-phosphoribosyltransferase from E. coli were obtained by the vapour-diffusion method and diffract to 2.7 Å. The ... Purification, crystallization and preliminary X-ray crystallographic analysis of ATP-phosphoribosyltransferase from Escherichia ...
1-(5-Phospho-d-ribosyl)-ATP:pyrophosphate phosphoribosyl-transferase. rxn00863. Histidine metabolism. l-histidinal:NAD + ... The ATP requirement for the synthesis of the pB40 plasmid was estimated based on the amount of ATP required for the synthesis ... 1-(5-Phospho-d-ribosyl)-ATP:pyrophosphate phosphoribosyl-transferase. rxn03135. Histidine metabolism. Imidazole-glycerol-3- ... ATP + l-aspartate =, ADP + 4-phospho-l-aspartate. rxn01643. Glycine, serine and threonine-cysteine and methionine-lysine ...
ATP phosphoribosyl transferase (HisG:HisZ) complex from Thermotoga maritima. PDB Compounds: (C:) ATP phosphoribosyltransferase ... Protein ATP phosphoribosyltransferase regulatory subunit HisZ [118058] (2 species). shares histidine-binding site with the ... d1usyc_ d.104.1.1 (C:) ATP phosphoribosyltransferase regulatory subunit HisZ {Thermotoga maritima [TaxId: 2336]} ...
ATP phosphoribosyltransferase. 80. SEQF2940,KI515728.1. SEQF2940_00082 jb [NA] [AA] 264/87. 81666-81403. Phosphoribosyl-ATP ... Lipid A export ATP-binding/permease protein MsbA. 25. SEQF2940,KI515728.1. SEQF2940_00025 jb [NA] [AA] 1743/580. 21694-23436. ... Lipid A export ATP-binding/permease protein MsbA. 26. SEQF2940,KI515728.1. SEQF2940_00026 jb [NA] [AA] 981/326. 23433-24413. ... ABC transporter ATP-binding protein NatA. 33. SEQF2940,KI515728.1. SEQF2940_00033 jb [NA] [AA] 570/189. 29757-30326. ...
ATP phosphoribosyltransferase. 307. SEQF2502,KB891039.1. SEQF2502_00308 jb [NA] [AA] 1131/376. 98085-96955. ATP ... ATP-dependent DNA helicase PcrA. 208. SEQF2502,KB891039.1. SEQF2502_00209 jb [NA] [AA] 831/276. 1091-261. Sugar phosphatase ... Choline transport ATP-binding protein OpuBA. 315. SEQF2502,KB891039.1. SEQF2502_00316 jb [NA] [AA] 450/149. 105566-105117. HTH- ... putative ABC transporter ATP-binding protein. 269. SEQF2502,KB891039.1. SEQF2502_00270 jb [NA] [AA] 1899/632. 59793-61691. ...
ATP-dependent serine phosphatase and EC 2.4.2.17: ATP phosphoribosyltransferase. Full lists of the differentially abundant KO ...
ATP phosphoribosyltransferase Current Synonym true false 131720015 Phosphoribosyl-ATP pyrophosphorylase Current Synonym true ... Adenosine triphosphate (ATP) phosphoribosyltransferase Current Synonym true false 2913505014 Adenosine triphosphate ... Adenosine triphosphate phosphoribosyltransferase (substance). Code System Preferred Concept Name. Adenosine triphosphate ...
ATP phosphoribosyltransferase. SMc00917. hisI. phosphoribosyl-ATP pyrophosphatase. SMc02568. SMc02307. hisE. phosphoribosyl-AMP ...
ATP phosphoribosyltransferase. 6. SEQF2884,KI530561.1. SEQF2884_00011 jb [NA] [AA] 1305/434. 10114-11418. Histidinol ... ATP-dependent DNA helicase Rep. 112. SEQF2884,KI530561.1. SEQF2884_00117 jb [NA] [AA] 453/150. 123481-123933. Deoxyuridine 5- ... Glutathione import ATP-binding protein GsiA. 72. SEQF2884,KI530561.1. SEQF2884_00077 jb [NA] [AA] 1053/350. 85780-84728. ... Lipoprotein-releasing system ATP-binding protein LolD. 184. SEQF2884,KI530561.1. SEQF2884_00192 jb [NA] [AA] 501/166. 209370- ...
ATP phosphoribosyltransferase activity. IEP. Enrichment. MF. GO:0003924. GTPase activity. IEP. Enrichment. ... proton-transporting ATP synthase activity, rotational mechanism. IEP. Enrichment. MF. GO:0050136. NADH dehydrogenase (quinone) ...
ATP-NADH kinase YEF1 (4) * Uracil phosphoribosyltransferase (4) * Folylpolyglutamate synthase (4) * Mitochondrial 2- ...
ATP phosphoribosyltransferase activity. IEP. Enrichment. MF. GO:0004424. imidazoleglycerol-phosphate dehydratase activity. IEP ...
ATP phosphoribosyltransferase. Compositional properties of ATP phosphoribosyltransferase (bottom) versus UniprotKB/SwissProt ( ...
ATP phosphoribosyltransferase activity. IEP. Neighborhood. MF. GO:0003958. NADPH-hemoprotein reductase activity. IEP. ... ATP dimethylallyltransferase activity. IEP. Neighborhood. MF. GO:0052623. ADP dimethylallyltransferase activity. IEP. ...
ATP-NADH kinase YEF1 (4) * Uracil phosphoribosyltransferase (4) * Folylpolyglutamate synthase (4) * Mitochondrial 2- ...
May allow the feedback regulation of ATP phosphoribosyltransferase activi... , canSARS ... ATP phosphoribosyltransferase regulatory subunit - Also known as HISZ_PSYA2, hisZ. Required for the first step of histidine ... May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine. Heteromultimer composed of HisG and ... May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine. Heteromultimer compose ... ...
70 ATP phosphoribosyl transferase EC 2.4.2.17 2(ch), 3(ch), 4 ... 73 Phosphoribosyl-ATP diphosphatase EC 3.6.1.31 2(ch), 3(ch), 4 ...
... suggesting that control of the pool of free His resides largely with ATP-PRT activity. Over-expression of ATP-PRT and increased ... ATP-PRT), the first enzyme in the pathway, was sufficient to increase the pool of free His by up to 42-fold in shoot tissue of ... Constitutive, CaMV 35S-driven over-expression of the cDNAs encoding either isoform of ATP-phosphoribosyltransferase ( ... Constitutive, CaMV 35S-driven over-expression of the cDNAs encoding either isoform of ATP-phosphoribosyltransferase (ATP-PRT), ...
ATP cobalamin adenoxyltransferase. ATP dependent 26S protease. ATP phosphoribosyltransferase. ATP sulfurylase. ATP synthase. ...
0.504049 INESSENTIAL HIS1 ATP phosphoribosyltransferase, histidine biosynthesis, ATP phosphoribosyltransferase, cell YDR334W - ... 2.674764 INESSENTIAL AAC1 mitochondrial ADP ATP translocator, ATP/ADP exchange, ATP/ADP antiporter, mitochondrial inner ... 1.533186 INESSENTIAL ATP17 ATP synthase subunit f, ATP synthesis coupled proton transport, hydrogen-transporting two-sector ... ATP synthesis coupled proton transport, structural protein, hydrogen-transporting ATP synthase, F0 sector YLR090W -2.764148 ...
Two of these were only present in GM plants from Campos Novos, the adenine phosphoribosyl transferase (APT), and the ATP- ... dependent Clp protease ATP-binding subunit ClpA (Clp-ClpA). APT works on adenine salvage in plants, while Clp-ClpA proteases ...
Nicotinate phosphoribosyltransferase Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D- ... Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides nicotinate phosphoribosyltransferase ... Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides nicotinate phosphoribosyltransferase ... TIM_barrel (CL0036) NAPRTase; Nicotinate phosphoribosyltransferase (NAPRTase) family (PF04095; HMM-score: 41.6) ...
ATP phosphoribosyltransferase. *: anthranilate phosphoribosyltransferase. *: nicotinate-nucleotide diphosphorylase ( ... nicotinate phosphoribosyltransferase. *: nicotinamide phosphoribosyltransferase. *: now EC 2.5.1.6. *: ... adenine phosphoribosyltransferase. *: hypoxanthine phosphoribosyltransferase. *: uracil phosphoribosyltransferase. *: orotate ... ATP citrate synthase. *: malate synthase. *: hydroxymethylglutaryl-CoA synthase. *: 2-hydroxyglutarate synthase. *: 3- ...
8] which results in accelerated hepatic breakdown of ATP and the generation of organic acids that compete with urate for ... Hypoxanthine guanine phosphoribosyltransferase (HGPRT) deficiency (Lesch-Nyhan syndrome): This is an inherited X-linked ... These include a complete deficiency of hypoxanthine guanine phosphoribosyltransferase (HGPRT) as in Lesch-Nyhan syndrome, ...
  • Enzyme adenine phosphoribosyltransferase aids in the salvaging of adenine formed and in its re-utilisation. (biologybard.com)
  • Other names in common use include phosphoribosyl-ATP pyrophosphorylase, adenosine triphosphate phosphoribosyltransferase, phosphoribosyladenosine triphosphate:pyrophosphate, phosphoribosyltransferase, phosphoribosyl ATP synthetase, phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase, phosphoribosyl-ATP:pyrophosphate-phosphoribosyl phosphotransferase, phosphoribosyladenosine triphosphate pyrophosphorylase, and phosphoribosyladenosine triphosphate synthetase. (wikipedia.org)
  • ATP or adenosine triphosphate, the energy currency Required for biological reactions and cellular metabolism is formed when adenosine attaches with three phosphate groups. (biologybard.com)
  • Adenosine Triphosphate (or ATP) is the unit of energy for your cells. (scienceofparkinsons.com)
  • They convert nutrients from food into Adenosine Triphosphate (or ATP). (scienceofparkinsons.com)
  • Once phosphorylated, ribose can become a subunit of ATP, NADH, and several other compounds that are critical to metabolism. (ecmdb.ca)
  • En fait la synthase forme et défait les doubles liaisons d'une protéine. (wikipedia.org)
  • ATP synthase peripheral stalk-membrane s. (gsea-msigdb.org)
  • In enzymology, an ATP phosphoribosyltransferase (EC 2.4.2.17) is an enzyme that catalyzes the chemical reaction 1-(5-phospho-D-ribosyl)-ATP + diphosphate ⇌ {\displaystyle \rightleftharpoons } ATP + 5-phospho-alpha-D-ribose 1-diphosphate Thus, the two substrates of this enzyme are 1-(5-phospho-D-ribosyl)-ATP and diphosphate, whereas its two products are ATP and 5-phospho-alpha-D-ribose 1-diphosphate. (wikipedia.org)
  • The systematic name of this enzyme class is 1-(5-phospho-D-ribosyl)-ATP:diphosphate phospho-alpha-D-ribosyl-transferase. (wikipedia.org)
  • The enzyme has been shown to be inhibited by 1-(5-phospho-D-ribosyl)-ATP, histidine, ppGpp (a signal associated with adverse environmental conditions) and ADP and AMP (which reflect the overall energy status of the cell). (wikipedia.org)
  • Constitutive, CaMV 35S-driven over-expression of the cDNAs encoding either isoform of ATP-phosphoribosyltransferase (ATP-PRT), the first enzyme in the pathway, was sufficient to increase the pool of free His by up to 42-fold in shoot tissue of Arabidopsis, with negligible effect on any other amino acid. (ox.ac.uk)
  • Deoxy-ATP (dATP) can reach toxic levels that inhibit ribonucleotide reductase, an enzyme essential for synthesis of DNA precursors. (medscape.com)
  • En biochimie , une ligase est une enzyme qui catalyse la jonction de deux molécules (en anglais ligation ) par de nouvelles liaisons covalentes avec hydrolyse concomitante de l' ATP ou d'autres molécules similaires. (wikipedia.org)
  • It is converted to nicotinamide mononucleotide (NMN) by the NAMPT (nicotinamide phosphoribosyltransferase) enzyme, and then to NAD + by the NMNAT (nicotinamide mononucleotide adenylytransferase) enzymes. (elifesciences.org)
  • Labelling of the enzyme with the glutamate analogue herbicide [ 14 C]phosphinothricin and with [y-32 P]ATP indicated that glutamine synthetase has eight reactive centers per molecule. (mpg.de)
  • Deficiency of hypoxanthine-guanine phosphoribosyltransferase (HPRT) activity is an inborn error of purine metabolism associated with uric acid overproduction and a continuum spectrum of neurological manifestations depending on the degree of the enzymatic deficiency. (biomedcentral.com)
  • Orotate is covalently linked with a phosphorylated ribosyl unit with Orotate phosphoribosyltransferase (aka "PRPP transferase") catalyzing reaction, yielding orotidine monophosphate (OMP). (pathbank.org)
  • On reaction with ATP produces 5-Phosphoribosyl-1-pyrophosphate (PRPP). (biologybard.com)
  • Purpose: The nicotinamide phosphoribosyltransferase (NAMPT) inhibitor, APO866, has been previously shown to have antileukemic activity in preclinical models, but its cytotoxicity in primary leukemia cells is frequently limited. (unige.it)
  • Histidine biosynthesis is an energetically expensive process and ATP phosphoribosyltransferase activity is subject to control at several levels. (wikipedia.org)
  • phosphoribosyl-ATP pyrophosphorylase. (expasy.org)
  • In the asparagine synthetase reaction, ATP is used to activate aspartate, forming beta-aspartyl-AMP. (smpdb.ca)
  • Compositional properties of ATP phosphoribosyltransferase (bottom) versus UniprotKB/SwissProt (top). (ucy.ac.cy)
  • Since acetogens grow by conversion of H 2 + CO 2 to acetate, the reaction has to be coupled to net synthesis of ATP [ 9 ]. (nature.com)
  • It is a member of the larger phosphoribosyltransferase superfamily of enzymes which catalyse the condensation of 5-phospho-alpha-D-ribose 1-diphosphate with nitrogenous bases in the presence of divalent metal ions. (wikipedia.org)
  • Importantly, it is a required co-factor in a process of passing hydrogen electrons from one protein to another, which is essential for the continued production of energy (in the form of ATP) by the mitochondria in cells. (scienceofparkinsons.com)
  • In this review, we focus on Niemann-Pick C1 Like 1 (NPC1L1) and a heterodimer of ATP-binding cassette transporter G5 and G8 (ABCG5/G8), both of which are responsible for intestinal cholesterol absorption and biliary cholesterol secretion, and discuss the relationship between these cholesterol transporters and lifestyle-related diseases. (go.jp)
  • Intracellular nicotinamide adenine dinucleotide (NAD+) and ATP levels, mitochondrial transmembrane potential (Δψm), markers of apoptosis and of endoplasmic reticulum (ER) stress were evaluated. (unige.it)
  • May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine. (icr.ac.uk)
  • In contrast, over-expression of cDNAs for seven other enzymes in the biosynthetic pathway had no effect on His content, suggesting that control of the pool of free His resides largely with ATP-PRT activity. (ox.ac.uk)
  • These enzymes synthesize NAD + from nicotinamide mononucleotide (NMN) and ATP, so two candidate mechanisms for how loss of NMNAT2 triggers axon degeneration have emerged: (1) the loss of NAD + or (2) the accumulation of NMN. (elifesciences.org)
  • ATP molecules are required to convert one mole of nitrogen to two ammonia molecules. (freezingblue.com)
  • ATP also forms one of the nucleotide monomeric units that produce a polymer of RNA and dATP formed by the addition of 3 phosphoric acids on deoxyadenosine, which is one of the constituent nucleotides that make up DNA. (biologybard.com)
  • Evidence will be presented that these two transitions, as well as the decline of NAD+ and ATP levels, are the root of cancer diseases. (hindawi.com)
  • Combining Pgp inhibitors with APO866 led to increased intracellular APO866 levels, compounded NAD+ and ATP shortage, and induced Δψm dissipation. (unige.it)
  • The cells composing the human body are similar to single-celled eukaryotes (existing 500,000 million years ago) although human cells can no longer survive on their own and generally do not use the primitive source of energy, e.g., substrate-level phosphorylation, to produce ATP. (hindawi.com)
  • ATP phosphoribosyltransferase is found in two distinct forms: a long form containing two catalytic domains and a C-terminal regulatory domain, and a short form in which the regulatory domain is missing. (wikipedia.org)
  • Over-expression of ATP-PRT and increased His content had a negative pleiotropic effect on plant biomass production in 35S:PRT1 lines, but this effect was not observed in 35S:PRT2 lines. (ox.ac.uk)