An enzyme that catalyzes the first step of the pathway for histidine biosynthesis in Salmonella typhimurium. ATP reacts reversibly with 5-phosphoribosyl-1-pyrophosphate to yield N-1-(5'-phosphoribosyl)-ATP and pyrophosphate. EC 2.4.2.17.
An enzyme that catalyzes the conversion of 5-phosphoribosyl-1-pyrophosphate and hypoxanthine, guanine, or 6-mercaptopurine to the corresponding 5'-mononucleotides and pyrophosphate. The enzyme is important in purine biosynthesis as well as central nervous system functions. Complete lack of enzyme activity is associated with the LESCH-NYHAN SYNDROME, while partial deficiency results in overproduction of uric acid. EC 2.4.2.8.
An enzyme catalyzing the formation of AMP from adenine and phosphoribosylpyrophosphate. It can act as a salvage enzyme for recycling of adenine into nucleic acids. EC 2.4.2.7.
Enzymes of the transferase class that catalyze the transfer of a pentose group from one compound to another.

An aminoacyl-tRNA synthetase paralog with a catalytic role in histidine biosynthesis. (1/30)

In addition to their essential catalytic role in protein biosynthesis, aminoacyl-tRNA synthetases participate in numerous other functions, including regulation of gene expression and amino acid biosynthesis via transamidation pathways. Herein, we describe a class of aminoacyl-tRNA synthetase-like (HisZ) proteins based on the catalytic core of the contemporary class II histidyl-tRNA synthetase whose members lack aminoacylation activity but are instead essential components of the first enzyme in histidine biosynthesis ATP phosphoribosyltransferase (HisG). Prediction of the function of HisZ in Lactococcus lactis was assisted by comparative genomics, a technique that revealed a link between the presence or the absence of HisZ and a systematic variation in the length of the HisG polypeptide. HisZ is required for histidine prototrophy, and three other lines of evidence support the direct involvement of HisZ in the transferase function. (i) Genetic experiments demonstrate that complementation of an in-frame deletion of HisG from Escherichia coli (which does not possess HisZ) requires both HisG and HisZ from L. lactis. (ii) Coelution of HisG and HisZ during affinity chromatography provides evidence of direct physical interaction. (iii) Both HisG and HisZ are required for catalysis of the ATP phosphoribosyltransferase reaction. This observation of a common protein domain linking amino acid biosynthesis and protein synthesis implies an early connection between the biosynthesis of amino acids and proteins.  (+info)

Specific binding of the first enzyme for histidine biosynthesis to the DNA of histidine operon. (2/30)

Studies were done to examine direct binding of the first enzyme of the histidine biosynthetic pathway (phosphoribosyltransferase) to 32P-labeled phi80dhis DNA and competition of this binding by unlabeled homologous DNA and by various preparations of unlabeled heterologous DNA, including that from a defective phi80 bacteriophage carrying the histidine operon with a deletion of part of its operator region. Our findings show that phosphoribosyltransferase binds specifically to site in or near the regulatory region of the histidine operon. The stability of the complex formed by interaction of the enzyme with the DNA was markedly decreased by the substrates of the enzyme and was slightly increased by the allosteric inhibitor, histidine. These findings are consistent with previous data that indicate that phosphoribosyltransferase plays a role in regulating expression of the histidine operon.  (+info)

Molecular dissection of the role of histidine in nickel hyperaccumulation in Thlaspi goesingense (Halacsy). (3/30)

To understand the role of free histidine (His) in Ni hyperaccumulation in Thlaspi goesingense, we investigated the regulation of His biosynthesis at both the molecular and biochemical levels. Three T. goesingense cDNAs encoding the following His biosynthetic enzymes, ATP phosphoribosyltransferase (THG1, GenBank accession no. AF003347), imidazoleglycerol phosphate dehydratase (THB1, GenBank accession no. AF023140), and histidinol dehydrogenase (THD1, GenBank accession no. AF023141) were isolated by functional complementation of Escherichia coli His auxotrophs. Northern analysis of THG1, THD1, and THB1 gene expression revealed that each gene is expressed in both roots and shoots, but at the concentrations and dosage times of Ni treatment used in this study, these genes failed to show any regulation by Ni. We were also unable to observe any increases in the concentration of free His in root, shoot, or xylem sap of T. goesingense in response to Ni exposure. X-ray absorption spectroscopy of root and shoot tissue from T. goesingense and the non-accumulator species Thlaspi arvense revealed no major differences in the coordination of Ni by His in these tissues. We therefore conclude that the Ni hyperaccumulation phenotype in T. goesingense is not determined by the overproduction of His in response to Ni.  (+info)

Molecular cloning and characterization of ATP-phosphoribosyl transferase from Arabidopsis, a key enzyme in the histidine biosynthetic pathway. (4/30)

We have characterized two isoforms of ATP-phosphoribosyl transferase (ATP-PRT) from Arabidopsis (AtATP-PRT1 [accession no. AB025251] and AtATP-PRT2), catalyzing the first step of the pathway of hisidine (His) biosynthesis. The primary structures deduced from AtATP-PRT1 and AtATP-PRT2 cDNAs share an overall amino acid identity of 74.6% and contain N-terminal chloroplast transit peptide sequences. DNA-blot analyses indicated that the ATP-PRTs in Arabidopsis are encoded by two separate genes with a closely similar gene structural organization. Both gene transcripts were detected throughout development, and protein-blot analysis revealed predominant accumulation of the AtATP-PRT proteins in Arabidopsis leaves. The His auxotrophy of a his1 mutant of Saccharomyces cerevisiae was suppressed by the transformation with AtATP-PRT1 and AtATP-PRT2 cDNAs, indicating that both isoforms are functionally active ATP-PRT enzymes. The K(m) values for ATP and phosphoribosyl pyrophosphate of the recombinant AtATP-PRT proteins were comparable to those of the native ATP-PRTs from higher plants and bacteria. It was demonstrated that the recombinant AtATP-PRTs were inhibited by L-His (50% inhibition of initial activity = 40-320 microM), suggesting that His biosynthesis was regulated in plants through feedback inhibition by L-His.  (+info)

Evidence against a covalent intermediate in the adenosine triphosphate phosphoribosyltransferase reaction of histidine biosynthesis. (5/30)

14C-Labeled 5-phospho-alpha-D-ribose-1-diphosphate (PRibPP) was synthesized and its interaction with adenosine triphosphate phosphoribosyltransferase was examined by gel filtration in a search for a form of this substrate covalently bound to the enzyme. Wide variation in solvent conditions gave little labeling of the enzyme. Heavy labeling was found only in the presence of the second substrate, ATP, and this was shown to arise from tightly but noncovalently bound product. Previous reports of a covalent intermediate in this enzymatic reaction probably were due to contaminating ATP in 5-phospho-alpha-D-ribose-1-diphosphate. Feedback inhibition of the enzyme by histidine was shown to occur at the step giving product or at some earlier step in the mechanism.  (+info)

trans-Recessive mutation in the first structural gene of the histidine operon that results in constitutive expression of the operon. (6/30)

The first enzyme for histidine biosynthesis, encoded in the hisG gene, is involved in regulation of expression of the histidine operon in Salmonella typhimurium. The studies reported here concern the question of how expression of the histidine operon is affected by a mutation in the hisG gene that alters the allosteric site of the first enzyme for histidine biosynthesis, rendering the enzyme completely resistant to inhibition by histidine. The intracellular concentrations of the enzymes encoded in the histidine operon in a strain carrying such a mutation on an episome and missing the chromosomal hisG gene are three- to fourfold higher than in a strain carrying a wild-type hisG gene on the episome. The histidine operon on such a strain fails to derepress in response to histidine limitation and fails to repress in response to excess histidine. Furthermore, utilizing other merodiploid strains, we demonstrate that the wild-type hisG gene is trans dominant to the mutant allele with respect to this regulatory phenomenon. Examination of the regulation of the histidine operon in strains carrying the feedback-resistant mutation in an episome and hisT and hisW mutations in the chromosome showed that the hisG regulatory mutation is epistatic to the hisT and hisW mutations. These data provide additional evidence that the first enzyme for histidine biosynthesis is involved in autogenous regulation of expression of the histidine operon.  (+info)

Derepression and repression of the histidine operon: role of the feedback site of the first enzyme. (7/30)

Thiazolealanine, a false feedback inhibitor, causes transient repression of the his operon previously derepressed by a severe histidine limitation in strains with a wild-type or feedback-hypersensitive first enzyme but not in feedback-resistant mutants. Since experiments reported here clearly demonstrate that thiazolealanine is not transferred to tRNAHis, it is proposed that this "transient repression" is effected through the interaction of thiazolealanine with the feedback site of the enzyme. Experiments in the presence of rifampin indicate that this thiazolealanine-mediated effect is exerted at the level of translation. We conclude that histidine (free), in addition to forming co-repressor, also represses the operon at the level of translation through feedback interaction with the first enzyme of the pathway (adenosine 5'-triphosphate phosphoribosyltransferase). Rates of derepression in feedback-resistant strains are roughly half of those observed in controls, suggesting a positive role played by a first enzyme with a normal but unoccupied feedback site. Some feedback-resistant mutants, in contrast to the wild type, were unable to exhibit derepression under histidine limitation caused by aminotriazole.  (+info)

Affinity labelling to - SH groups in adenosine - triphosphate - phosphoribosyl transferase with the dinitrophenyl group from S-dinitrophenyl-6-mercaptopurine-riboside 5'-phosphate. (8/30)

Adenosine-triphosphate-phosphoribosyl transferase from Escherichia coli reacts with S-dinitrophenyl-6-mercaptopurine-riboside 5'-phosphate. In this reaction the dinitrophenyl group becomes attached to the enzyme, while the nucleotide is split off. Most aliphatic high and low-molecular-weight-SH compounds react with the thioether in the opposite way, i.e. bind the nucleotide and split off dinitrothiophenol. It appears that the dinitrophenyl moiety of the thioether interacts with the enzyme in a specific way, and that this interaction activates the bond between the dinitrophenyl group and the sulfur atom. In support of this it was found that dinitrophenol inhibits the transferase reaction with half maximal effect at 0.4 mM. The inhibition is competitive with ATP. Dinitrophenol also competes with ATP in binding studies.  (+info)

ATP phosphoribosyltransferase (ATP-PRT, or adenine phosphoribosyltransferase) is an enzyme involved in the purine nucleotide biosynthesis pathway. The enzyme catalyzes the conversion of ATP and 5-phosphoribosyl-1-pyrophosphate (PRPP) to adenosine monophosphate (AMP) and pyrophosphate (PPi). This reaction is part of the salvage pathway, which recycles purines by converting free purine bases back into nucleotides. A deficiency in ATP-PRT can lead to a rare genetic disorder known as adenine phosphoribosyltransferase deficiency or APRT deficiency, which is characterized by the accumulation of 2,8-dihydroxyadenine crystals in the renal tubules, resulting in kidney stones and potential kidney damage.

Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is an enzyme that plays a crucial role in the salvage pathway of nucleotide synthesis. This enzyme catalyzes the conversion of hypoxanthine and guanine to their respective nucleotides, inosine monophosphate (IMP) and guanosine monophosphate (GMP), by transferring the phosphoribosyl group from 5-phosphoribosyl-1 pyrophosphate (PRPP) to the purine bases.

HGPRT deficiency is a genetic disorder known as Lesch-Nyhan syndrome, which is characterized by mental retardation, self-mutilation, spasticity, and uric acid overproduction due to the accumulation of hypoxanthine and guanine. This disorder is caused by mutations in the HPRT1 gene, leading to a decrease or absence of HGPRT enzyme activity.

Adenine Phosphoribosyltransferase (APRT) is an enzyme that plays a crucial role in the metabolism of purines, specifically adenine, in the body. The enzyme catalyzes the conversion of adenine to AMP (adenosine monophosphate) by transferring a phosphoribosyl group from 5-phosphoribosyl-1-pyrophosphate (PRPP) to adenine.

Deficiency in APRT can lead to a rare genetic disorder known as Adenine Phosphoribosyltransferase Deficiency or APRT Deficiency. This condition results in the accumulation of 2,8-dihydroxyadenine (DHA) crystals in the renal tubules, which can cause kidney stones and chronic kidney disease. Proper diagnosis and management, including dietary modifications and medication, are essential to prevent complications associated with APRT Deficiency.

Pentosyltransferases are a group of enzymes that catalyze the transfer of a pentose (a sugar containing five carbon atoms) molecule from one compound to another. These enzymes play important roles in various biochemical pathways, including the biosynthesis of nucleotides, glycoproteins, and other complex carbohydrates.

One example of a pentosyltransferase is the enzyme that catalyzes the addition of a ribose sugar to form a glycosidic bond with a purine or pyrimidine base during the biosynthesis of nucleotides, which are the building blocks of DNA and RNA.

Another example is the enzyme that adds xylose residues to proteins during the formation of glycoproteins, which are proteins that contain covalently attached carbohydrate chains. These enzymes are essential for many biological processes and have been implicated in various diseases, including cancer and neurodegenerative disorders.

... phosphoribosyl ATP synthetase, phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase, phosphoribosyl-ATP:pyrophosphate- ... an ATP phosphoribosyltransferase (EC 2.4.2.17) is an enzyme that catalyzes the chemical reaction 1-(5-phospho-D-ribosyl)-ATP + ... ATP phosphoribosyltransferase is found in two distinct forms: a long form containing two catalytic domains and a C-terminal ... Histidine biosynthesis is an energetically expensive process and ATP phosphoribosyltransferase activity is subject to control ...
... by the enzyme ATP-phosphoribosyl transferase. ATP-phosphoribosyl transferase is indicated by His1 in the image. His4 gene ... ATP-phosphoribosyl transferase (shown as His1 in the image on the right). ATP-phosphoribosyl transferase is the rate ... This pathway requires energy in order to occur therefore, the presence of ATP activates the first enzyme of the pathway, ... The first reaction of histidine biosynthesis is the condensation of PRPP and adenosine triphosphate (ATP) ...
... catalyzed by ATP-phosphoribosyl transferase. Phosphoribosyl-ATP converts to phosphoribosyl-AMP (PRAMP). His4 then catalyzes the ... In the asparagine synthetase reaction, ATP is used to activate aspartate, forming β-aspartyl-AMP. Glutamine donates an ammonium ... The enzyme asparagine synthetase produces asparagine, AMP, glutamate, and pyrophosphate from aspartate, glutamine, and ATP. ... is strongly inhibited by α-ketoglutarate feedback inhibition and can be inhibited by DPNH as well high concentrations of ATP. ...
The step of histidine biosynthesis is the condensation of ATP and PRPP by ATP-phosphoribosyl transferase, the rate determining ... R5P and its derivatives serve as precursors to many biomolecules, including DNA, RNA, ATP, coenzyme A, FAD (Flavin adenine ... The build up is caused by a deficiency of the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT), which leads to ... During the purine salvage pathway, phosphoribosyltransferases add PRPP to bases. PRPP also plays an important role in ...
ATP phosphoribosyltransferase regulatory subunit, LTA synthase family protein, type I DNA topoisomerase, nicotinate ... phosphoribosyltransferase, bifunctional hydroxymethylpyrimidine kinase/phospho- methylpyrimidine kinase, single-stranded-DNA- ...
... anthranilate phosphoribosyltransferase MeSH D08.811.913.400.725.200 - ATP phosphoribosyltransferase MeSH D08.811.913.400. ... 725.450 - hypoxanthine phosphoribosyltransferase MeSH D08.811.913.400.725.700 - orotate phosphoribosyltransferase MeSH D08.811. ... atp citrate (pro-s)-lyase MeSH D08.811.913.050.350 - carnitine acyltransferases MeSH D08.811.913.050.350.170 - carnitine O- ... atp-dependent proteases MeSH D08.811.277.656.149.200 - endopeptidase clp MeSH D08.811.277.656.149.500 - protease la MeSH ...
... trafficking GUK1 Guanylate kinase transfers phosphate from ATP to GMP HPRT Hypoxanthine-guanine phosphoribosyltransferase ... 001689 Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c ATP5H NM_006356 Homo sapiens ATP ... ATP reservoir) Cytidine deaminase questionable: not present in very high levels at all CPNE1 ENSA (gene) FTH1 Heavy chain of ... 004046 Homo sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, alpha ATP5B NM_001686 ATP5C1 NM_005174 ATP5D NM_ ...
Uridylate (UMP) is later converted to UDP via phosphorylation by UMP kinase and ATP and then nucleoside diphosphate kinase ... Like other pyrimidine phosphoribosyltransferases, orotate phosphoribosyltransferase has a flexible loop that moves to position ... Orotate phosphoribosyltransferase (OPRTase) or orotic acid phosphoribosyltransferase is an enzyme involved in pyrimidine ... Pyrimidine phosphoribosyltransferases such as orotate phosphoribosyltransferase activate their substrates by forming SN1-like ...
iNAMPT can also catalyze the synthesis of NMN from phosphoribosyl pyrophosphate (PRPP) when ATP is present. eNAMPT has been ... Nicotinamide phosphoribosyltransferase (NAmPRTase or NAMPT), formerly known as pre-B-cell colony-enhancing factor 1 (PBEF1) or ... Grolla AA, Travelli C, Genazzani AA, Sethi JK (July 2016). "Extracellular nicotinamide phosphoribosyltransferase, a new cancer ... September 2006). "Crystal structure of visfatin/pre-B cell colony-enhancing factor 1/nicotinamide phosphoribosyltransferase, ...
ATP phosphoribosyltransferase EC 2.4.2.18: anthranilate phosphoribosyltransferase EC 2.4.2.19: nicotinate-nucleotide ... uracil phosphoribosyltransferase EC 2.4.2.10: orotate phosphoribosyltransferase EC 2.4.2.11: now EC 6.3.4.21 nicotinate ... nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase EC 2.4.2.22: xanthine phosphoribosyltransferase EC 2.4. ... adenine phosphoribosyltransferase EC 2.4.2.8: hypoxanthine phosphoribosyltransferase EC 2.4.2.9: ...
ATP, and H2O, whereas its four products are nicotinate D-ribonucleotide, diphosphate, ADP, and phosphate. This enzyme belongs ... In enzymology, a nicotinate phosphoribosyltransferase (EC 6.3.4.21) is an enzyme that catalyzes the chemical reaction ... Kosaka A, Spivey HO, Gholson RK (1971). "Nicotinate phosphoribosyltransferase of yeast. Purification and properties". J. Biol. ... nicotinate + 5-phospho-α-D-ribose 1-diphosphate + ATP + H2O ⇌ {\displaystyle \rightleftharpoons } nicotinate D-ribonucleotide ...
The synthesis of IMP, (precursor to GMP and GTP, and to AMP and ATP) also requires THF, and also can be bypassed. In this case ... hypoxanthine-guanine phosphoribosyltransferase (HGPRT) reacts hypoxanthine absorbed from the medium with PRPP, liberating ...
ATP-dependent helicase, tRNA (cytidine(34)-2′-O)-methyltransferase, glutamine-fructose-6-phosphate transaminase (isomerizing), ... uracil phosphoribosyltransferase, L-histidine N(alpha)-methyltransferase, DUF58 domain-containing protein, NADH-quinone ...
... have been shown to be vital cofactors in cellular oxidation/reduction reactions and ATP synthesis. Classic NAD+ synthesis ... and an NAM-based pathway involving the enzyme Nicotinamide phosphoribosyltransferase (NAMPT) and the formation of nicotinamide ...
The histidine biosynthesis pathway involves the reaction between PRPP and ATP, which activates the latter to ring cleavage. ... Decreased levels of hypoxanthine guanine phosphoribosyl transferase (HGPRT) causes this accumulation, as PRPP is a substrate ... Salmonella typhimurium nicotinate phosphoribosyltransferase". Journal of Biological Chemistry. 268 (34): 26004-26010. doi: ... with the first step being N-alkylation of anthranilic acid catalysed by the enzyme anthranilate phosphoribosyltransferase. ...
ATP stimulates production of GTP, while GTP stimulates production of ATP. This cross regulation keeps the relative amounts of ... Lesch-Nyhan syndrome is caused by a deficiency in hypoxanthine-guanine phosphoribosyltransferase or HGPRT, the enzyme that ... ATP, a purine nucleotide, is an activator of pyrimidine synthesis, while CTP, a pyrimidine nucleotide, is an inhibitor of ... XMP is then converted into GMP by using the hydrolysis of 1 ATP and the conversion of glutamine to glutamate. AMP and GMP can ...
Both steps are fueled by ATP hydrolysis: ATP + UMP → ADP + UDP UDP + ATP → UTP + ADP CTP is subsequently formed by the ... Orotate phosphoribosyltransferase (PRPP transferase) catalyzes the net reaction yielding orotidine monophosphate (OMP): Orotate ... Glutamine is the NH3 donor and the reaction is fueled by ATP hydrolysis, too: UTP + Glutamine + ATP + H2O → CTP + ADP + Pi ... to PRPP by reacting it with ATP. The reaction is unusual in that a pyrophosphoryl group is directly transferred from ATP to C1 ...
Cancer cells are known to become resistant to DAP by losing their adenine phosphoribosyltransferase (APRT) function, a process ... ATP + dGMP + L-aspartate = (d)ADP + phosphate + 2-aminodeoxyadenylosuccinate (dSMP) The resulting dSMP is processed by host ... 6-diaminopurine and 6-methylpurine that affect adenine phosphoribosyltransferase in Escherichia coli K-12]". Genetika. 13 (10 ...
Importantly, the process in the organelle has no net ATP synthesis. This ATP comes later from processes outside of the ... These enzymes found in the glycosome to help with synthesis are guanine and adenine phosphoribosyl transferase, hypoxanthine, ... This energy metabolism generates ATP through the process of glycolysis. The glycosome is a host of the main glycolytic enzymes ... the glycosomes attached to the myofibrils seem to serve the myosin by providing energy substrates for generation of ATP through ...
Conversely, PRPP and ATP act as positive effectors that enhance the enzyme's activity. Modulating the pyrimidine metabolism ... UMPS is a bifunctional enzyme consisting of orotate phosphoribosyltransferase (OPRT) and orotidine monophosphate decarboxylase ...
ATP) EC 6.5.1.2: DNA ligase (NAD+) EC 6.5.1.3: RNA ligase (ATP) EC 6.5.1.4: RNA 3′-terminal-phosphate cyclase (ATP) EC 6.5.1.5 ... nicotinate phosphoribosyltransferase EC 6.3.4.22: tRNAIle2-agmatinylcytidine synthase * EC 6.3.4.23: formate- ... RNA 3′-terminal-phosphate cyclase (GTP) * EC 6.5.1.6: DNA ligase (ATP or NAD+) * EC 6.5.1.7: DNA ligase (ATP, ADP or GTP) * EC ... As ATP is not hydrolysed during the reaction, the classification of the enzyme as a ligase was incorrect EC 6.3.5.9: ...
This contrasts with eukaryotic DNA ligases, which use ATP to form the DNA-AMP intermediate. Li et al. have found that NAD+ ... The first step, and the rate-limiting enzyme in the salvage pathway is nicotinamide phosphoribosyltransferase (NAMPT), which ... Yamboliev IA, Smyth LM, Durnin L, Dai Y, Mutafova-Yambolieva VN (2009). "Storage and secretion of beta-NAD, ATP and dopamine in ... In most organisms, this enzyme uses adenosine triphosphate (ATP) as the source of the phosphate group, although several ...
fGAR + L-Glutamine + ATP → fGAM + L-Glutamate + ADP + Pi The fifth is catalyzed by AIR synthetase (FGAM cyclase). fGAM + ATP → ... The enzyme adenine phosphoribosyltransferase (APRT) salvages adenine. The enzyme hypoxanthine-guanine phosphoribosyltransferase ... CAIR + L-Aspartate + ATP → SAICAR + ADP + Pi The eight is catalyzed by adenylosuccinate lyase. SAICAR → AICAR + Fumarate The ... PRPP + L-Glutamine + H2O → PRA + L-Glutamate + PPi In the second step react PRA, glycine and ATP to create GAR, ADP, and ...
The first step for joining an amino acid to its corresponding tRNA is the formation of aminoacyl-AMP: Amino acid + ATP ↽ − − ⇀ ... It is caused by the absence of hypoxanthine-guanine phosphoribosyltransferase, which is a necessary enzyme for purine ... The following step requires the activation of glycine by the addition of a phosphate group from ATP. GAR synthetase performs ... High energy molecules, such as ATP, have three phosphates. Often, the terminal phosphate is split off during hydrolysis and ...
ATP synthase (EC 3.6.3.14) Kynureninase EC 3.7.1.3 EC 3.8.1.3 Haloacetate dehalogenase EC 3.9.1.1: Phosphoamidase EC 3.9.1.2: ... EC 2.4.2 Hypoxanthine-guanine phosphoribosyltransferase EC 2.4.2.8 Category:EC 2.5 Category:EC 2.5.1 Thiaminase EC 2.5.1.2 ... ATP-hydrolysing) Serine racemase Category:EC 5.1.2 Mandelate racemase Category:EC 5.1.3 UDP-glucose 4-epimerase Category:EC 5.1 ...
Functional Consequences of ATP- and dATP-Induced Oligomerization of the Large Subunit†". Biochemistry. 41 (2): 462-74. doi: ... "Different Oligomeric States are Involved in the Allosteric Behavior of Uracil Phosphoribosyltransferase from Escherichia Coli ... W in Complex with Nonhydrolyzable ATP Analogues Reveal a Putative Active Conformation of the Enzyme as a Result of Domain ... Dependent Malic Enzyme by ATP and Fumarate". Structure. 10 (7): 951-60. doi:10.1016/S0969-2126(02)00788-8. PMID 12121650. ...
Alternatively to ATP, GTP has been shown to act comparably as a phosphate donor. This promiscuity enables the important role ... "Epstein-Barr virus encoded nuclear protein EBNA-3 binds a novel human uridine kinase/uracil phosphoribosyltransferase". BMC ... hydroxyl group on the substrate and activates it to attack the γ-phosphorus of ATP. Structural analyses have shown that the ...
... phosphoribosyl ATP synthetase, phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase, phosphoribosyl-ATP:pyrophosphate- ... an ATP phosphoribosyltransferase (EC 2.4.2.17) is an enzyme that catalyzes the chemical reaction 1-(5-phospho-D-ribosyl)-ATP + ... ATP phosphoribosyltransferase is found in two distinct forms: a long form containing two catalytic domains and a C-terminal ... Histidine biosynthesis is an energetically expensive process and ATP phosphoribosyltransferase activity is subject to control ...
Weak coupling of atp hydrolysis to the chemical equilibrium of human nicotinamide phosphoribosyltransferase. Biochemistry 2008 ... Cellular NAD+ level and, thereby, the NAD+/NADH ratio affect not only ATP synthesis, but also generation of reactive oxygen ... In a study on human fibroblasts, NAM treatment lowered cellular ATP level by approximately 10% after 3-day treatment at 5 mM [ ... Hara, N.; Yamada, K.; Shibata, T.; Osago, H.; Tsuchiya, M. Nicotinamide phosphoribosyltransferase/visfatin does not catalyze ...
Crystal structure, steady-state and pre-steady-state kinetics of Acinetobacter baumannii ATP phosphoribosyltransferase. Read, B ... Allosteric activation unveils protein-mass modulation of ATP phosphoribosyltransferase product release. Read, B., Mitchell, J. ...
ATP phosphoribosyltransferase activity. IEP. Enrichment. MF. GO:0004181. metallocarboxypeptidase activity. IEP. Enrichment. ...
The adenosine-triphosphate phosphoribosyltransferase (ATP-PRTase) facilities the committed first step of the histidine ... ATP phosphoribosyltransferase from symbiont Entomomyces delphacidicola invovled in histidine biosynthesis of Nilaparvata lugens ... In the current study, a putative ATP-PRTase was cloned and verified to be of E. delphacidicola origin (EdePRTase). The ... The results provide a better understanding of EdePRTase and the encoded functional ATP-PRTase enzyme regulation in N. lugens ...
Anthranilate phosphoribosyltransferase. ATPPRT. ATP phosphoribosyltransferase. GLUPRT. Glutamine phosphoribosyldiphosphate ...
8, 9] ATP accumulates in erythrocytes, and ADP, guanosine triphosphate (GTP), and ATP accumulate in lymphocytes. Deoxy-ATP ( ... AMP = adenosine monophosphate, APRT = adenine phosphoribosyltransferase, GMP = guanosine monophosphate, HGPRT = hypoxanthine- ... AMP = adenosine monophosphate, APRT = adenine phosphoribosyltransferase, GMP = guanosine monophosphate, HGPRT = hypoxanthine- ... guanine phosphoribosyltransferase, IMP = inosine monophosphate, NP = nucleoside phosphorylase, PPriboseP = 5-phosphorylribose-1 ...
... is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then ... the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate (PubMed: ... Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D- ... Nicotinate Phosphoribosyltransferase. DTO Classes. Protein. / Enzyme. / Transferase. / Glycosyltransferase. / Nicotinate ...
ATP, NAD), signaling (eg, GTP, cAMP, cGMP), and, along with pyrimidines, RNA and DNA production. Purines may be synthesized de ... deficiency of hypoxanthine-guanine phosphoribosyltransferase (complete deficiency is Lesch-Nyhan syndrome Lesch-Nyhan syndrome ...
ATP synthase, alpha subunit, C-terminal; ATP synthase, F1 complex, gamma subunit; ATP synthase, F1 complex, delta/epsilon ... ","Dunaliella sp.","Nicotinate/nicotinamide phosphoribosyltransferase; Nicotinamide phosphoribosyltransferase, N-terminal ... ATP synthase, alpha subunit, C-terminal; V-ATPase proteolipid subunit C-like domain; ATP synthase, F0 complex, subunit A [ ... ATP synthase, alpha subunit, C-terminal; ATP synthase, F1 complex, gamma subunit; V-ATPase proteolipid subunit C-like domain; ...
PHATRDRAFT_22803 atp-binding protein of abc transporter (ABCD_peroxisomal_ALDP) PHATRDRAFT_22803 - atp-binding protein of abc ... PHATRDRAFT_26336 uracil phosphoribosyltransferase (PRTases_typeI) PHATRDRAFT_26336 - uracil phosphoribosyltransferase (PRTases_ ...
ABCG8: ATP binding cassette subfamily G member 8. *ABHD5: abhydrolase domain containing 5, lysophosphatidic acid ... APRT: adenine phosphoribosyltransferase. *APTX: aprataxin. *AQP2: aquaporin 2. *AR: androgen receptor. *ARFGEF2: ADP ... ABCG5: ATP binding cassette subfamily G member 5. * ... ABCA1: ATP binding cassette subfamily A member 1. *ABCA3: ATP ... ABCG2: ATP binding cassette subfamily G member 2 (JR blood group). * ...
An enzyme that catalyzes the conversion of ATP and thymidine to ADP and thymidine 5-phosphate. Deoxyuridine can also act as an ... Orotate Phosphoribosyltransferase. The enzyme catalyzing the formation of orotidine-5-phosphoric acid (orotidylic acid) from ... An enzyme that catalyzes the formation of ADP plus AMP from adenosine plus ATP. It can serve as a salvage mechanism for ... Adenine Phosphoribosyltransferase. An enzyme catalyzing the formation of AMP from adenine and phosphoribosylpyrophosphate. It ...
... adenine phosphoribosyltransferase insufficiency, hypoxanthine-guanine phosphoribosyltransferase lack, and also innate ... Srs2 can be an ATP-dependent anti-recombinase which downregulates Hours by simply make an effort to dismantling Rad51 filaments ...
Tovar S, Paeger L, Hess S, Da M, Ac H, Hs B, Hampel B, Pj A, Evers N, Buning H et al (2013) K(Atp)-channel-dependent regulation ... Relative values were expressed in relation to hypoxanthine-guanine phosphoribosyl-transferase (Hprt, Mm03024075_m1) levels. We ...
Human ABCC10(ATP Binding Cassette Transporter C10) ELISA Kit. *Human AMIGO1(Adhesion Molecule With Ig Like Domain Protein 1) ... Human UPRT(Uracil Phosphoribosyltransferase) ELISA Kit. *Human WNT4(Wingless Type MMTV Integration Site Family, Member 4) ELISA ...
Maximal ATP synthesis (ATPmax), mM/s. 0.80 ± 0.26. 0.78 ± 0.18. 0.89 ± 0.34. 0.88 ± 0.30. 0.17. 0.72. 0.92. ... nicotinamide phosphoribosyltransferase; NQO1, NAD(P)H quinone dehydrogenase 1; NRF1, nuclear respiratory factor 1; OGG1, 8- ... Main Outcomes: In vivo mitochondrial function [maximal ATP synthesis rate (ATPmax), ATPflux/O2 (P/O)] was determined by 31P- ... or ATP turnover (ATPflux)/O2 (P/O)][8,9] and produce less reactive oxygen species (ROS).[10] Parts of this hypothesis, however ...
Mouse ABCA9(ATP Binding Cassette Transporter A9) ELISA Kit. *Rat HPD(4-Hydroxyphenylpyruvate Dioxygenase) ELISA Kit ... Mouse QPRT(Quinolinate Phosphoribosyltransferase) ELISA Kit. *Human H1F0(H1 Histone Family, Member 0) ELISA Kit ...
Mouse ABCA1(ATP Binding Cassette Transporter A1) ELISA Kit. *Mouse ABCG2(ATP Binding Cassette Transporter G2) ELISA Kit ... Human HPRT1(Hypoxanthine Phosphoribosyltransferase 1) ELISA Kit. *Human HRH4(Histamine Receptor H4) ELISA Kit ...
Mouse ABCA1(ATP Binding Cassette Transporter A1) ELISA Kit. *Mouse ABCG2(ATP Binding Cassette Transporter G2) ELISA Kit ... Human HPRT1(Hypoxanthine Phosphoribosyltransferase 1) ELISA Kit. *Human HRH4(Histamine Receptor H4) ELISA Kit ...
Mouse ABCA1(ATP Binding Cassette Transporter A1) ELISA Kit. *Mouse ABCG2(ATP Binding Cassette Transporter G2) ELISA Kit ... Human HPRT1(Hypoxanthine Phosphoribosyltransferase 1) ELISA Kit. *Human HRH4(Histamine Receptor H4) ELISA Kit ...
Human ABCC10(ATP Binding Cassette Transporter C10) ELISA Kit. *Human SCXB(Scleraxis Homolog B) ELISA Kit ... Human UPRT(Uracil Phosphoribosyltransferase) ELISA Kit. *Human HSPA12B(Heat Shock 70kDa Protein 12B) ELISA Kit ...
Orotidine-5-Phosphate Pyrophosphorylase use Orotate Phosphoribosyltransferase Orotidylate Decarboxylase use Orotidine-5- ... Organic Anion Transporters, ATP-Dependent Organic Anion Transporters, Sodium-Dependent Organic Anion Transporters, Sodium- ...
Orotidine-5-Phosphate Pyrophosphorylase use Orotate Phosphoribosyltransferase Orotidylate Decarboxylase use Orotidine-5- ... Organic Anion Transporters, ATP-Dependent Organic Anion Transporters, Sodium-Dependent Organic Anion Transporters, Sodium- ...
  • These include a complete deficiency of hypoxanthine guanine phosphoribosyltransferase (HGPRT) as in Lesch-Nyhan syndrome, partial deficiency of HGPRT (Kelley-Seegmiller syndrome), and increased production of 5-phospho-alpha-d-ribosyl pyrophosphate (PRPP) activity. (medscape.com)
  • Other names in common use include phosphoribosyl-ATP pyrophosphorylase, adenosine triphosphate phosphoribosyltransferase, phosphoribosyladenosine triphosphate:pyrophosphate, phosphoribosyltransferase, phosphoribosyl ATP synthetase, phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase, phosphoribosyl-ATP:pyrophosphate-phosphoribosyl phosphotransferase, phosphoribosyladenosine triphosphate pyrophosphorylase, and phosphoribosyladenosine triphosphate synthetase. (wikipedia.org)
  • In enzymology, an ATP phosphoribosyltransferase (EC 2.4.2.17) is an enzyme that catalyzes the chemical reaction 1-(5-phospho-D-ribosyl)-ATP + diphosphate ⇌ {\displaystyle \rightleftharpoons } ATP + 5-phospho-alpha-D-ribose 1-diphosphate Thus, the two substrates of this enzyme are 1-(5-phospho-D-ribosyl)-ATP and diphosphate, whereas its two products are ATP and 5-phospho-alpha-D-ribose 1-diphosphate. (wikipedia.org)
  • An enzyme that catalyzes the formation of carbamoyl phosphate from ATP, carbon dioxide, and glutamine. (lookformedical.com)
  • Deoxy-ATP (dATP) can reach toxic levels that inhibit ribonucleotide reductase, an enzyme essential for synthesis of DNA precursors. (medscape.com)
  • Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate (PubMed:17604275, PubMed:21742010, PubMed:26042198). (nih.gov)
  • In vivo mitochondrial function [maximal ATP synthesis rate (ATPmax), ATPflux/O 2 (P/O)] was determined by 31 P-magnetic resonance spectroscopy and optical spectroscopy, and body composition was determined by dual-energy X-ray absorptiometry. (medscape.com)
  • The most common cause under this group is alcohol consumption,[6] which results in accelerated hepatic breakdown of ATP and the generation of organic acids that compete with urate for tubular secretion. (medscape.com)
  • Histidine biosynthesis is an energetically expensive process and ATP phosphoribosyltransferase activity is subject to control at several levels. (wikipedia.org)
  • It is a member of the larger phosphoribosyltransferase superfamily of enzymes which catalyse the condensation of 5-phospho-alpha-D-ribose 1-diphosphate with nitrogenous bases in the presence of divalent metal ions. (wikipedia.org)
  • 15. Inhibition of Nicotinamide Phosphoribosyltransferase (NAMPT), an Enzyme Essential for NAD+ Biosynthesis, Leads to Altered Carbohydrate Metabolism in Cancer Cells. (nih.gov)
  • 16. Weak coupling of ATP hydrolysis to the chemical equilibrium of human nicotinamide phosphoribosyltransferase. (nih.gov)
  • 19. Inhibition of nicotinamide phosphoribosyltransferase protects against acute pancreatitis via modulating macrophage polarization and its related metabolites. (nih.gov)
  • A70: NAM is converted to nicotinamide mononucleotide (NMN) by nicotinamide phosphoribosyltransferase (NAMPT, EC 2.4.2.12) (PMID: 19403820). (nih.gov)
  • A17: The rate-limiting protein in the conversion of NAM back to NAD+ is nicotinamide phosphoribosyltransferase (NAMPT), which catalyzes the reaction of nicotinamide into nicotinamide mononucleotide (NMN). (nih.gov)
  • Twenty-four hours after treatment, ROS production, cell survival, the content of glutathione (GSH) and adenosine triphosphate (ATP), and cellular bioenergetics were measured. (hindawi.com)
  • Hypoxanthine-guanine phosphoribosyltransferases (HGPRTs) are enzymes that are critical for the salvage of preformed purines, catalyzing the formation of inosine monophosphate (IMP) and guanosine monophosphate (GMP) from the nucleobases hypoxanthine and guanine, respectively. (nih.gov)
  • Enzima que cataliza el primer paso de la vía de la biosíntesis de histidina en Salmonella typhimurium. (bvsalud.org)
  • Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate (PubMed:17604275, PubMed:21742010, PubMed:26042198). (nih.gov)
  • ATP reacts reversibly with 5-phosphoribosyl-1-pyrophosphate to yield N-1-(5'-phosphoribosyl)- ATP and pyrophosphate. (nih.gov)
  • A71: Nicotinamide mononucleotide adenylyltransferase (NMNAT, EC 2.7.7.1) utilizes NMN and ATP to produce NAD+ and pyrophosphate (PPi) (PMID: 19403820). (nih.gov)
  • Discovery of novel nitrobenzothiazole inhibitors for Mycobacterium tuberculosis ATP phosphoribosyl transferase (HisG) through virtual screening. (nih.gov)