A superfamily of large integral ATP-binding cassette membrane proteins whose expression pattern is consistent with a role in lipid (cholesterol) efflux. It is implicated in TANGIER DISEASE characterized by accumulation of cholesteryl ester in various tissues.
A family of MEMBRANE TRANSPORT PROTEINS that require ATP hydrolysis for the transport of substrates across membranes. The protein family derives its name from the ATP-binding domain found on the protein.
The most abundant protein component of HIGH DENSITY LIPOPROTEINS or HDL. This protein serves as an acceptor for CHOLESTEROL released from cells thus promoting efflux of cholesterol to HDL then to the LIVER for excretion from the body (reverse cholesterol transport). It also acts as a cofactor for LECITHIN CHOLESTEROL ACYLTRANSFERASE that forms CHOLESTEROL ESTERS on the HDL particles. Mutations of this gene APOA1 cause HDL deficiency, such as in FAMILIAL ALPHA LIPOPROTEIN DEFICIENCY DISEASE and in some patients with TANGIER DISEASE.
A sequence-related subfamily of ATP-BINDING CASSETTE TRANSPORTERS that actively transport organic substrates. Although considered organic anion transporters, a subset of proteins in this family have also been shown to convey drug resistance to neutral organic drugs. Their cellular function may have clinical significance for CHEMOTHERAPY in that they transport a variety of ANTINEOPLASTIC AGENTS. Overexpression of proteins in this class by NEOPLASMS is considered a possible mechanism in the development of multidrug resistance (DRUG RESISTANCE, MULTIPLE). Although similar in function to P-GLYCOPROTEINS, the proteins in this class share little sequence homology to the p-glycoprotein family of proteins.
An autosomal recessively inherited disorder caused by mutation of ATP-BINDING CASSETTE TRANSPORTERS involved in cellular cholesterol removal (reverse-cholesterol transport). It is characterized by near absence of ALPHA-LIPOPROTEINS (high-density lipoproteins) in blood. The massive tissue deposition of cholesterol esters results in HEPATOMEGALY; SPLENOMEGALY; RETINITIS PIGMENTOSA; large orange tonsils; and often sensory POLYNEUROPATHY. The disorder was first found among inhabitants of Tangier Island in the Chesapeake Bay, MD.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A broad category of receptor-like proteins that may play a role in transcriptional-regulation in the CELL NUCLEUS. Many of these proteins are similar in structure to known NUCLEAR RECEPTORS but appear to lack a functional ligand-binding domain, while in other cases the specific ligands have yet to be identified.
The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
A 170-kDa transmembrane glycoprotein from the superfamily of ATP-BINDING CASSETTE TRANSPORTERS. It serves as an ATP-dependent efflux pump for a variety of chemicals, including many ANTINEOPLASTIC AGENTS. Overexpression of this glycoprotein is associated with multidrug resistance (see DRUG RESISTANCE, MULTIPLE).
A class of lipoproteins of small size (4-13 nm) and dense (greater than 1.063 g/ml) particles. HDL lipoproteins, synthesized in the liver without a lipid core, accumulate cholesterol esters from peripheral tissues and transport them to the liver for re-utilization or elimination from the body (the reverse cholesterol transport). Their major protein component is APOLIPOPROTEIN A-I. HDL also shuttle APOLIPOPROTEINS C and APOLIPOPROTEINS E to and from triglyceride-rich lipoproteins during their catabolism. HDL plasma level has been inversely correlated with the risk of cardiovascular diseases.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Lipid-protein complexes involved in the transportation and metabolism of lipids in the body. They are spherical particles consisting of a hydrophobic core of TRIGLYCERIDES and CHOLESTEROL ESTERS surrounded by a layer of hydrophilic free CHOLESTEROL; PHOSPHOLIPIDS; and APOLIPOPROTEINS. Lipoproteins are classified by their varying buoyant density and sizes.
The process of cleaving a chemical compound by the addition of a molecule of water.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
Transport proteins that carry specific substances in the blood or across cell membranes.
Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.
Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Established cell cultures that have the potential to propagate indefinitely.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
Proteins found in any species of fungus.
A subfamily of transmembrane proteins from the superfamily of ATP-BINDING CASSETTE TRANSPORTERS that are closely related in sequence to P-GLYCOPROTEIN. When overexpressed, they function as ATP-dependent efflux pumps able to extrude lipophilic drugs, especially ANTINEOPLASTIC AGENTS, from cells causing multidrug resistance (DRUG RESISTANCE, MULTIPLE). Although P-Glycoproteins share functional similarities to MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS they are two distinct subclasses of ATP-BINDING CASSETTE TRANSPORTERS, and have little sequence homology.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Simultaneous resistance to several structurally and functionally distinct drugs.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Elements of limited time intervals, contributing to particular results or situations.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Proteins found in any species of bacterium.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
The ability of fungi to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance phenotype may be attributed to multiple gene mutations.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A family of sterols commonly found in plants and plant oils. Alpha-, beta-, and gamma-isomers have been characterized.
The rate dynamics in chemical or physical systems.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
A family of scavenger receptors that are predominately localized to CAVEOLAE of the PLASMA MEMBRANE and bind HIGH DENSITY LIPOPROTEINS.
Proteins obtained from ESCHERICHIA COLI.
An oligopeptide produced by various bacteria which acts as a protease inhibitor.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Proteins involved in the transport of organic anions. They play an important role in the elimination of a variety of endogenous substances, xenobiotics and their metabolites from the body.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
An anthracenedione-derived antineoplastic agent.
An antidiabetic sulfonylurea derivative with actions similar to those of chlorpropamide.
A class of organic compounds known as STEROLS or STEROIDS derived from plants.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Chemical substances that are foreign to the biological system. They include naturally occurring compounds, drugs, environmental agents, carcinogens, insecticides, etc.
The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Resistance or diminished response of a neoplasm to an antineoplastic agent in humans, animals, or cell or tissue cultures.
The process by which two molecules of the same chemical composition form a condensation product or polymer.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
An X-linked recessive disorder characterized by the accumulation of saturated very long chain fatty acids in the LYSOSOMES of ADRENAL CORTEX and the white matter of CENTRAL NERVOUS SYSTEM. This disease occurs almost exclusively in the males. Clinical features include the childhood onset of ATAXIA; NEUROBEHAVIORAL MANIFESTATIONS; HYPERPIGMENTATION; ADRENAL INSUFFICIENCY; SEIZURES; MUSCLE SPASTICITY; and DEMENTIA. The slowly progressive adult form is called adrenomyeloneuropathy. The defective gene ABCD1 is located at Xq28, and encodes the adrenoleukodystrophy protein (ATP-BINDING CASSETTE TRANSPORTERS).
Proteins prepared by recombinant DNA technology.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Steroids with a hydroxyl group at C-3 and most of the skeleton of cholestane. Additional carbon atoms may be present in the side chain. (IUPAC Steroid Nomenclature, 1987)
A dextrodisaccharide from malt and starch. It is used as a sweetening agent and fermentable intermediate in brewing. (Grant & Hackh's Chemical Dictionary, 5th ed)
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The product of conjugation of cholic acid with taurine. Its sodium salt is the chief ingredient of the bile of carnivorous animals. It acts as a detergent to solubilize fats for absorption and is itself absorbed. It is used as a cholagogue and cholerectic.
Cholesterol which is contained in or bound to high-density lipoproteins (HDL), including CHOLESTEROL ESTERS and free cholesterol.
The relationship between the dose of an administered drug and the response of the organism to the drug.
A thickening and loss of elasticity of the walls of ARTERIES that occurs with formation of ATHEROSCLEROTIC PLAQUES within the ARTERIAL INTIMA.
A ubiquitously expressed glucose transporter that is important for constitutive, basal GLUCOSE transport. It is predominately expressed in ENDOTHELIAL CELLS and ERYTHROCYTES at the BLOOD-BRAIN BARRIER and is responsible for GLUCOSE entry into the BRAIN.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Diminished or failed response of an organism, disease or tissue to the intended effectiveness of a chemical or drug. It should be differentiated from DRUG TOLERANCE which is the progressive diminution of the susceptibility of a human or animal to the effects of a drug, as a result of continued administration.
Membrane transporters that co-transport two or more dissimilar molecules in the same direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.
Sodium chloride-dependent neurotransmitter symporters located primarily on the PLASMA MEMBRANE of serotonergic neurons. They are different than SEROTONIN RECEPTORS, which signal cellular responses to SEROTONIN. They remove SEROTONIN from the EXTRACELLULAR SPACE by high affinity reuptake into PRESYNAPTIC TERMINALS. Regulates signal amplitude and duration at serotonergic synapses and is the site of action of the SEROTONIN UPTAKE INHIBITORS.
A family of proteins involved in the transport of monocarboxylic acids such as LACTIC ACID and PYRUVIC ACID across cellular membranes.
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
Sodium chloride-dependent neurotransmitter symporters located primarily on the PLASMA MEMBRANE of dopaminergic neurons. They remove DOPAMINE from the EXTRACELLULAR SPACE by high affinity reuptake into PRESYNAPTIC TERMINALS and are the target of DOPAMINE UPTAKE INHIBITORS.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A cell line derived from cultured tumor cells.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Bactericidal cationic quaternary ammonium surfactant used as a topical anti-infective agent. It is an ingredient in medicaments, deodorants, mouthwashes, etc., and is used to disinfect apparatus, etc., in the food processing and pharmaceutical industries, in surgery, and also as a preservative. The compound is toxic orally as a result of neuromuscular blockade.
Lipid-laden macrophages originating from monocytes or from smooth muscle cells.
Membrane proteins whose primary function is to facilitate the transport of negatively charged molecules (anions) across a biological membrane.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
A glutamate plasma membrane transporter protein found in ASTROCYTES and in the LIVER.
Oxyvanadium ions in various states of oxidation. They act primarily as ion transport inhibitors due to their inhibition of Na(+)-, K(+)-, and Ca(+)-ATPase transport systems. They also have insulin-like action, positive inotropic action on cardiac ventricular muscle, and other metabolic effects.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A neuronal and epithelial type glutamate plasma membrane transporter protein.
Membrane proteins whose primary function is to facilitate the transport of positively charged molecules (cations) across a biological membrane.
5'-Adenylic acid, monoanhydride with imidodiphosphoric acid. An analog of ATP, in which the oxygen atom bridging the beta to the gamma phosphate is replaced by a nitrogen atom. It is a potent competitive inhibitor of soluble and membrane-bound mitochondrial ATPase and also inhibits ATP-dependent reactions of oxidative phosphorylation.
A family of POTASSIUM and SODIUM-dependent acidic amino acid transporters that demonstrate a high affinity for GLUTAMIC ACID and ASPARTIC ACID. Several variants of this system are found in neuronal tissue.
Organic or inorganic compounds that contain the -N3 group.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A chloride channel that regulates secretion in many exocrine tissues. Abnormalities in the CFTR gene have been shown to cause cystic fibrosis. (Hum Genet 1994;93(4):364-8)
An organic cation transporter found in kidney. It is localized to the basal lateral membrane and is likely to be involved in the renal secretion of organic cations.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A glial type glutamate plasma membrane transporter protein found predominately in ASTROCYTES. It is also expressed in HEART and SKELETAL MUSCLE and in the PLACENTA.
Sodium chloride-dependent neurotransmitter symporters located primarily on the PLASMA MEMBRANE of noradrenergic neurons. They remove NOREPINEPHRINE from the EXTRACELLULAR SPACE by high affinity reuptake into PRESYNAPTIC TERMINALS. It regulates signal amplitude and duration at noradrenergic synapses and is the target of ADRENERGIC UPTAKE INHIBITORS.
Salts and esters of CHOLIC ACID.

Alternative sulfonylurea receptor expression defines metabolic sensitivity of K-ATP channels in dopaminergic midbrain neurons. (1/7249)

ATP-sensitive potassium (K-ATP) channels couple the metabolic state to cellular excitability in various tissues. Several isoforms of the K-ATP channel subunits, the sulfonylurea receptor (SUR) and inwardly rectifying K channel (Kir6.X), have been cloned, but the molecular composition and functional diversity of native neuronal K-ATP channels remain unresolved. We combined functional analysis of K-ATP channels with expression profiling of K-ATP subunits at the level of single substantia nigra (SN) neurons in mouse brain slices using an RT-multiplex PCR protocol. In contrast to GABAergic neurons, single dopaminergic SN neurons displayed alternative co-expression of either SUR1, SUR2B or both SUR isoforms with Kir6.2. Dopaminergic SN neurons expressed alternative K-ATP channel species distinguished by significant differences in sulfonylurea affinity and metabolic sensitivity. In single dopaminergic SN neurons, co-expression of SUR1 + Kir6.2, but not of SUR2B + Kir6.2, correlated with functional K-ATP channels highly sensitive to metabolic inhibition. In contrast to wild-type, surviving dopaminergic SN neurons of homozygous weaver mouse exclusively expressed SUR1 + Kir6.2 during the active period of dopaminergic neurodegeneration. Therefore, alternative expression of K-ATP channel subunits defines the differential response to metabolic stress and constitutes a novel candidate mechanism for the differential vulnerability of dopaminergic neurons in response to respiratory chain dysfunction in Parkinson's disease.  (+info)

Inward rectification in KATP channels: a pH switch in the pore. (2/7249)

Inward-rectifier potassium channels (Kir channels) stabilize the resting membrane potential and set a threshold for excitation in many types of cell. This function arises from voltage-dependent rectification of these channels due to blockage by intracellular polyamines. In all Kir channels studied to date, the voltage-dependence of rectification is either strong or weak. Here we show that in cardiac as well as in cloned KATP channels (Kir6.2 + sulfonylurea receptor) polyamine-mediated rectification is not fixed but changes with intracellular pH in the physiological range: inward-rectification is prominent at basic pH, while at acidic pH rectification is very weak. The pH-dependence of polyamine block is specific for KATP as shown in experiments with other Kir channels. Systematic mutagenesis revealed a titratable C-terminal histidine residue (H216) in Kir6.2 to be the structural determinant, and electrostatic interaction between this residue and polyamines was shown to be the molecular mechanism underlying pH-dependent rectification. This pH-dependent block of KATP channels may represent a novel and direct link between excitation and intracellular pH.  (+info)

Overexpression of the multidrug resistance-associated protein (MRP1) in human heavy metal-selected tumor cells. (3/7249)

Cellular and molecular mechanisms involved in the resistance to cytotoxic heavy metals remain largely to be characterized in mammalian cells. To this end, we have analyzed a metal-resistant variant of the human lung cancer GLC4 cell line that we have selected by a step-wise procedure in potassium antimony tartrate. Antimony-selected cells, termed GLC4/Sb30 cells, poorly accumulated antimony through an enhanced cellular efflux of metal, thus suggesting up-regulation of a membrane export system in these cells. Indeed, GLC4/Sb30 cells were found to display a functional overexpression of the multidrug resistance-associated protein MRP1, a drug export pump, as demonstrated by Western blotting, reverse transcriptase-polymerase chain reaction and calcein accumulation assays. Moreover, MK571, a potent inhibitor of MRP1 activity, was found to markedly down-modulate resistance of GLC4/Sb30 cells to antimony and to decrease cellular export of the metal. Taken together, our data support the conclusion that overexpression of functional MRP1 likely represents one major mechanism by which human cells can escape the cytotoxic effects of heavy metals.  (+info)

Neural modulation of cephalexin intestinal absorption through the di- and tripeptide brush border transporter of rat jejunum in vivo. (4/7249)

Intestinal absorption of beta-lactamine antibiotics (e.g., cefixime and cephalexin) has been shown to proceed through the dipeptide carrier system. In a previous study, nifedipine (NFP), an L-type calcium channel blocker, enhanced the absorption of cefixime in vivo but not in vitro, and it was suggested that neural mechanisms might be involved in the effect of NFP. The aim of the present study was to assess the involvement of the nervous system on the intestinal absorption of cephalexin (CFX). To investigate this, we used a single-pass jejunal perfusion technique in rats. NFP and diltiazem enhanced approximately 2-fold the plasma levels of CFX in treated rats versus untreated controls. NFP also increased approximately 2-fold the CFX level in portal plasma and increased urinary excretion of CFX, thus indicating that CFX did effectively increase CFX intestinal absorption. Perfusing high concentrations of dipeptides in the jejunal lumen competitively reduced CFX absorption and inhibited the enhancement of CFX absorption produced by NFP. Hexamethonium and lidocaine inhibited the effect of NFP, whereas atropine, capsaicin, clonidine, and isoproterenol enhanced CFX absorption by the same order of magnitude as NFP. Thus, complex neural networks can modulate the function of the intestinal di- and tripeptide transporter. Sympathetic noradrenergic fibers, intestinal sensory neurons, and nicotinic synapses are involved in the increase of CFX absorption produced by NFP.  (+info)

Expression of atrC - encoding a novel member of the ATP binding cassette transporter family in Aspergillus nidulans - is sensitive to cycloheximide. (5/7249)

A new member of the ABC superfamily of transmembrane proteins in Aspergillus nidulans has been cloned and characterized. The topology of conserved motifs subgroups AtrC in the P-glycoprotein cluster of ABC permeases, the members of this subfamily, are known to participate in multidrug resistance (MDR) in diverse organisms. Alignment results display significant amino acid similarity to AfuMDR1 and AflMDR1 from Aspergillus fumigatus and flavus, respectively. Northern analysis reveals that atrC mRNA levels are 10-fold increased in response to cycloheximide. Evidence for the existence of eight additional hitherto unpublished ABC transporter proteins in A. nidulans is provided.  (+info)

MalK forms a dimer independent of its assembly into the MalFGK2 ATP-binding cassette transporter of Escherichia coli. (6/7249)

The maltose transport complex (MTC) is a member of the ATP-binding cassette superfamily of membrane transport proteins and is a model for understanding the folding and assembly of hetero-oligomeric membrane protein complexes. The MTC is made up of two integral membrane proteins, MalF and MalG, and a peripheral membrane protein, MalK. These proteins associate with a stoichiometry of 1:1:2 to form the complex MalFGK2. In our studies of the oligomerization of this complex, we have shown that the ATP-binding component, MalK, forms a dimer in the absence of MalF and MalG. Epitope-tagged MalK coimmunoprecipitated with wild-type MalK, indicating that the MalK protein forms an oligomer. The relative amounts of tagged and wild-type MalK that were present in the whole cell extracts and in the immunoprecipitated complexes show that the MalK oligomer is a dimer. These hetero-oligomers can also be formed in vitro by mixing two extracts, each containing either tagged or wild-type MalK. The dimerization of MalK was also demonstrated in vivo using the bacteriophage lambda repressor fusion assay. The formation of a MalK dimer in the absence of MalF and MalG may represent an initial step in the assembly pathway of the MTC.  (+info)

Glucose-receptive neurones in the rat ventromedial hypothalamus express KATP channels composed of Kir6.1 and SUR1 subunits. (7/7249)

1. Patch-clamp recordings were made from rat ventromedial hypothalamic neurones in slices of brain tissue in vitro. In cell-attached recordings, removal of extracellular glucose or metabolic inhibition with sodium azide reduced the firing rate of a subpopulation of cells through the activation of a 65 pS channel that was blocked by the sulphonylureas tolbutamide and glibenclamide. 2. In whole-cell patch-clamp recordings, in the absence of ATP in the electrode solution, glucose-receptive neurones gradually hyperpolarized due to the induction of an outward current at -60 mV. This outward current and the resultant hyperpolarization were blocked by the sulphonylureas tolbutamide and glibenclamide. 3. In recordings where the electrode solution contained 4 mM ATP, this outward current was not observed. Under these conditions, 500 microM diazoxide was found to induce an outward current that was blocked by tolbutamide. 4. In cell-attached recordings diazoxide and the active fragment of leptin (leptin 22-56) reduced the firing rate of glucose-receptive neurones by the activation of a channel with similar properties to that induced by removal of extracellular glucose. 5. Reverse transcription followed by the polymerase chain reaction using cytoplasm from single glucose-receptive neurones demonstrated the expression of the ATP-sensitive potassium (KATP) channel subunits Kir6.1 and SUR1 but not Kir6.2 or SUR2. 6. It is concluded that glucose-receptive neurones within the rat ventromedial hypothalamus exhibit a KATP channel current with pharmacological and molecular properties similar to those reported in other tissues.  (+info)

Functional analysis of the promoter of the yeast SNQ2 gene encoding a multidrug resistance transporter that confers the resistance to 4-nitroquinoline N-oxide. (8/7249)

The yeast gene SNQ2, which encodes a multidrug resistance ABC superfamily protein, is required for resistance to the mutagen 4-nitroquinoline N-oxide (4-NQO). The expression of the SNQ2 gene is under the control of a regulatory network that involves the transcription factor Yrr1p, as well as Pdr1p/Pdr3p (Cui et al., Mol. Microbiol., 29, 1307-1315 (1998)). By 5'-deletion analysis of the promoter by using SNQ2-lacZ fusion constructs, four regions: -745 to -639 (region I), -639 to -578 (region II), -548 to -533 (region III) and -533 to -485 (region IV) were found to be important for SNQ2 expression. Genetic analysis suggested that the site in region IV was responsible for the Yrr1p-mediated SNQ2 expression. A consensus motif known for the binding of Pdr1p/Pdr3p (PDRE) was not found in region IV.  (+info)

KAMISAKO, T. and OGAWA, H. (2005), Alteration of the expression of adenosine triphosphate-binding cassette transporters associated with bile acid and cholesterol transport in the rat liver and intestine during cholestasis. Journal of Gastroenterology and Hepatology, 20: 1429-1434. doi: 10.1111/j.1440-1746.2005.03950.x ...
Title: Structure and Function of the Human Breast Cancer Resistance Protein (BCRP/ABCG2). VOLUME: 11 ISSUE: 7. Author(s):Zhanglin Ni, Zsolt Bikadi, Mark F. Rosenberg and Qingcheng Mao. Affiliation:Department of Pharmaceutics,School of Pharmacy, University of Washington, Health Science Building H272, 1959 NE Pacific Street , Seattle, Washington 98195-7610, USA.. Keywords:Breast cancer resistance protein, BCRP, ATP-binding cassette transporter, ABCG2, multidrug resistance, drug disposition, homology model, mutation analysis, BCRP/ABCG2, mitoxantrone-resistant human cancer cell lines, MXR, human placenta, ABCP, nucleotide binding domains, membrane spanning domains, MSDs, affinity constants, SAR, QSAR, flavonoids, tamoxifen analogues, cyclindependent kinase inhibitors, tariquidar analogues, FTC analogues, 2D-QSAR, CoMFA, CoMSIA, 3D-QSAR, MIFs, HEK cells, Pichia pastoris, Lactococcus lactis, fluorescence resonance energy transfer v, MODELLER. Abstract: The human breast cancer resistance protein ...
ABCC4 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (ABCC4), transcript variant 2 - 10 µg - OriGene - cdna clones
TY - JOUR. T1 - A novel mutation in the ABCR gene in four patients with autosomal recessive Stargardt disease. AU - Zhang, Kang. AU - Garibaldi, Daniel C.. AU - Kniazeva, Marina. AU - Albini, Thomas. AU - Chiang, Michael F.. AU - Kerrigan, Michelle. AU - Sunness, Janet S.. AU - Han, Min. AU - Allikmets, Rando. PY - 1999/12/1. Y1 - 1999/12/1. N2 - PURPOSE: To identify additional mutations in the ABCR gene and describe the clinical features of four affected siblings with autosomal recessive Stargardt disease. METHODS: A cohort of eight siblings was identified for study. Four of these individuals were diagnosed with Stargardt disease based on clinical evaluation and fluorescein angiography. Blood samples were obtained from seven of eight siblings, including all those affected. All 50 exons of the ABCR gene were analyzed by single-stranded confirmation polymorphism analysis, followed by direct sequencing of observed variants, to identify mutations in the ABCR gene. RESULTS: We identified a ...
Overexpression of adenosine triphosphate-binding cassette (ABC) transport proteins is emerging seeing that a crucial contributor to anticancer medication level of resistance. assays. eIF4G mRNA degradation was accelerated in cells transfected with miR-503 mimics. Furthermore, it had been demonstrated that eIF4G and ABC translation protein were downregulated in MCF-7/ADR cells after transfection with miR-503 significantly. It was discovered that miR-503 mimics could sensitize the cells to treatment with ADM, TAX and TAM. These findings confirmed for the very first time that eIF4G acted as an integral element in MCF-7/ADR cells, and Riociguat could end up being a competent agent for preventing and reversing multi-drug resistance in breast malignancy. (11) determinded that cisplatin-resistance cells upregulated MRP1 when compared with sensitive MCF-7 cells. The eukaryotic initiation factor (eIF) 4F complex consists of three proteins: cap-binding protein eIF4E, scaffolding protein eIF4G and ...
Title: Interaction of Probenecid with the Breast Cancer Resistance Protein Transporter (BCRP/ABCG2). VOLUME: 3 ISSUE: 4. Author(s):G. Merino, R. Real, A. J. Molina, M. M. Pulido, J. G. Prieto and A. I. Alvarez. Affiliation:University of León,Department of Physiology, Faculty of Veterinary Medicine, Campus deVegazana 24071 León, Spain.. Keywords:Probenecid, Breast Cancer Resistance Protein, P-glycoprotein, transport, inhibition. Abstract: Probenecid is used as a uricosuric agent in the treatment of chronic gout and as an adjunct to enhance antibiotic levels in the blood. For research purposes, it is used as a prototypic inhibitor of organic anion transporters and MRPs, including MPR2. The purpose of this research is to study the interaction of probenecid with two other important transporters of the ATP-binding cassette family, Breast Cancer Resistance Protein (BCRP) and P-glycoprotein. These drug efflux transporters are present in the intestine, liver and other organs, and they affect the ...
The transport of molecules across lipid membranes is an essential function of all living organisms. One of the families of genes that have evolved to carry out this function is that which encodes the ATP-binding cassette proteins. These molecules use active transport to pump specific molecules acros …
During the past years, we and others discovered a series of human ATP-binding cassette (ABC) transporters, now referred to as ABC A-subfamily transporters. Recently, a novel testis-specific ABC A transporter, Abca17, has been cloned in rodent. In this study, we report the identification and characterization of the human ortholog of rodent Abca17. The novel human ABC A-transporter gene on chromosome 16p13.3 is ubiquitously expressed with highest expression in glandular tissues and the heart. The new ABC transporter gene exhibits striking nucleotide sequence homology with the recently cloned mouse (58%) and rat Abca17 (51%), respectively, and is located in the syntenic region of mouse Abca17 indicating that it represents the human ortholog of rodent Abca17. However, unlike in the mouse, the full-length ABCA17 transcript (4.3 kb) contains numerous mutations that preclude its translation into a bona fide ABC transporter protein strongly suggesting that the human ABCA17 gene is a transcribed pseudogene
[51 Pages Report] Check for Discount on ATP Binding Cassette Sub Family A Member 1 (ATP Binding Cassette Transporter 1 or ABC 1 or ATP Binding Cassette 1 or Cholesterol Efflux Regulatory Protein or ABCA1) - Pipeline Review, H1 2016 report by Global Markets Direct. Global Markets Directs, ATP Binding Cassette Sub...
TY - JOUR. T1 - Functions of the breast cancer resistance protein (BCRP/ABCG2) in chemotherapy. AU - Noguchi, Kohji. AU - Katayama, Kazuhiro. AU - Mitsuhashi, Junko. AU - Sugimoto, Yoshikazu. N1 - Funding Information: We apologize that, due to space limitations, we could not cite the excellent work of many investigators. This work was supported by a grant-in-aid for Cancer Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.. PY - 2009/1/31. Y1 - 2009/1/31. N2 - The breast cancer resistance protein, BCRP/ABCG2, is a half-molecule ATP-binding cassette transporter that facilitates the efflux of various anticancer agents from the cell, including 7-ethyl-10-hydroxycamptothecin, topotecan and mitoxantrone. The expression of BCRP can thus confer a multidrug resistance phenotype in cancer cells, and its transporter activity is involved in the in vivo efficacy of chemotherapeutic agents. Thus, the elucidation of the substrate preferences and structural relationships ...
ATP-binding cassette transporters (ABC transporters) are members of a transport system superfamily that is one of the largest and is possibly one of the oldest families with representatives in all extant phyla from prokaryotes to humans. ABC transporters often consist of multiple subunits, one or two of which are transmembrane proteins and one or two of which are membrane-associated ATPases. The ATPase subunits utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to energize the translocation of various substrates across membranes, either for uptake or for export of the substrate. Most but not all uptake systems also have an extracytoplasmic receptor, a solute binding protein. Some homologous ATPases function in non-transport-related processes such as translation of RNA and DNA repair. ABC transporters are considered to be with the ABC superfamily based on the sequence and organization of their ATP-binding cassette (ABC) domains, even though the integral membrane proteins ...
We characterized a new human ATP-binding cassette (ABC) transporter gene that is highly expressed in the placenta. The gene, ABCP, produces two transcripts that differ at the 5′ end and encode the same 655-amino acid protein. The predicted protein is closely related to the Drosophila white and yeast ADP1 genes and is a member of a subfamily that includes several multidrug resistance transporters. ABCP, white, and ADP1 all have a single ATP-binding domain at the NH2 terminus and a single COOH-terminal set of transmembrane segments. ABCP maps to human chromosome 4q22, between the markers D4S2462 and D4S1557, and the murine gene (Abcp) is located on chromosome 6 28-29 cM from the centromere. ABCP defines a new syntenic segment between human chromosome 4 and mouse chromosome 6. The abundant expression of this gene in the placenta suggests that the protein product has an important role in transport of specific molecule(s) into or out of this tissue.. ...
With the well-established link between serum cholesterol levels and cardiovascular disease and the availability of effective cholesterol-lowering drugs, cholesterol screening has rapidly become a routine part of health care. Yet, much remains to be learned about how cholesterol levels are regulated at the cellular level (see the Perspective by Brown et al.). Now, Najafi-Shoushtari et al. (p. 1566, published online 13 May) and Rayner et al. (p. 1570, published online 13 May) have discovered a new molecular player in cholesterol control-a small noncoding RNA that, intriguingly, is embedded within the genes coding for sterol regulatory element-binding proteins (SREBPs), transcription factors already known to regulate cholesterol levels. This microRNA, called miR-33, represses expression of the adenosine triphosphate-binding cassette transporter A1, a protein that regulates synthesis of high-density lipoprotein (HDL, or good cholesterol) and that helps to remove bad cholesterol from the blood. ...
1. Higgins CF. ABC transporters: from microorganisms to man. Annu Rev Cell Biol. 1992;8: 67-113. doi: 10.1146/annurev.cb.08.110192.000435 1282354. 2. Rees DC, Johnson E, Lewinson O. ABC transporters: the power to change. Nat Rev Mol Cell Biol. 2009;10: 218-227. doi: 10.1038/nrm2646 19234479. 3. Dean M, Rzhetsky A, Allikmets R. The human ATP-binding cassette (ABC) transporter superfamily. Genome Res. 2001;11: 1156-1166. doi: 10.1101/gr.184901 11435397. 4. Klein I, Sarkadi B, Varadi A. An inventory of the human ABC proteins. Biochim Biophys Acta-Biomembr. 1999;1461: 237-262. doi: 10.1016/S0005-2736(99)00161-3. 5. Seeger MA, van Veen HW. Molecular basis of multidrug transport by ABC transporters. Biochim Biophys Acta BBA-Proteins Proteomics. 2009;1794: 725-737. doi: 10.1016/j.bbapap.2008.12.004 19135557. 6. Holland IB, A. Blight M. ABC-ATPases, adaptable energy generators fuelling transmembrane movement of a variety of molecules in organisms from bacteria to humans. J Mol Biol. 1999;293: 381-399. ...
With the well-established link between serum cholesterol levels and cardiovascular disease and the availability of effective cholesterol-lowering drugs, cholesterol screening has rapidly become a routine part of health care. Yet, much remains to be learned about how cholesterol levels are regulated at the cellular level (see the Perspective by Brown et al.). Now, Najafi-Shoushtari et al. and Rayner et al. have discovered a new molecular player in cholesterol control-a small noncoding RNA that, intriguingly, is embedded within the genes coding for sterol regulatory element-binding proteins (SREBPs), transcription factors already known to regulate cholesterol levels. This microRNA, called miR-33, represses expression of the adenosine triphosphate-binding cassette transporter A1, a protein that regulates synthesis of high-density lipoprotein (HDL, or good cholesterol) and that helps to remove bad cholesterol from the blood. Reducing the levels of miR-33 in mice boosted serum HDL levels, ...
Membrane transport proteins are known to influence the absorption, distribution, metabolism, excretion and toxicity (ADMET) of drugs. At the onset of this thesis work, only a few structure-activity models, in general describing P-glycoprotein (Pgp/ABCB1) interactions, were developed using small datasets with little structural diversity. In this thesis, drug-transport protein interactions were explored using large, diverse datasets representing the chemical space of orally administered registered drugs. Focus was set on the ATP-binding cassette (ABC) transport proteins expressed in the canalicular membrane of human hepatocytes.. The inhibition of the ABC transport proteins multidrug-resistance associated protein 2 (MRP2/ABCC2) and bile salt export pump (BSEP/ABCB11) was experimentally investigated using membrane vesicles from cells overexpressing the investigated proteins and sandwich cultured human hepatocytes (SCHH). Several previously unknown inhibitors were identified for both of the proteins ...
TY - JOUR. T1 - ATP-binding cassette transporter A1 (ABCA1) R219K (G1051A, rs2230806) polymorphism and serum high-density lipoprotein cholesterol levels in a large Japanese population. T2 - Cross-sectional data from the Daiko study. AU - Mokuno, Junichiro. AU - Hishida, Asahi. AU - Morita, Emi. AU - Sasakabe, Tae. AU - Hattori, Yuta. AU - Suma, Shino. AU - Okada, Rieko. AU - Kawai, Sayo. AU - Naito, Mariko. AU - Wakai, Kenji. PY - 2015/4/11. Y1 - 2015/4/11. N2 - Among polymorphisms in ATP-binding cassette transporter Al (ABCA1) gene, the available evidence demonstrates that the ABCA1 R219K polymorphism (G1051A, rs2230806) K allele is associated with a higher high-density lipoprotein cholesterol (HDL- C) level and may play a protective role against coronary artery disease (CAD) risk in Asians and Caucasians. The findings from many underpowered studies from Asian countries (n=71-597), however, still remain inconsistent. The objective of this study was to overcome the limitations of previous ...
K11085 msbA; ATP-binding cassette, subfamily B, bacterial MsbA [EC:3.6.3.-] K11085 msbA; ATP-binding cassette, subfamily B, bacterial MsbA [EC:3.6.3.-] K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06160 pvdE; putative pyoverdin transport system ATP-binding/permease protein K15738 uup; ABC transport system ATP-binding/permease protein K21397 K21397; ABC transport system ATP-binding/permease protein K21397 K21397; ABC transport system ATP-binding/permease protein K23163 sbp; sulfate/thiosulfate transport system substrate-binding protein K02046 cysU; ...
Introduction Recently, the ATP-binding cassette transporter BCRP1/ABCG2 has been shown to regulate the function and survival of side population cells, which have been identified in various organs including heart and have stem cell properties. In addition, previous studies have revealed that BCRP1/ABCG2 is also expressed in endothelial cells of capillaries and arterioles in heart. This study was performed to clarify the role of BCRP1/ABCG2 in cardiac repair after myocardial infarction (MI).. Methods and Results MI was induced in 8- to 12-week-old wild-type (WT) mice (n=51) and Bcrp1/Abcg2 knock-out (KO) mice (n=60) by ligating the left anterior descending artery. At 28 days after MI, the survival rate was significantly lower in KO mice than in WT mice (28.3% versus 74.5%, p=0.0001). The main cause of death in KO mice was cardiac rupture (CR) (67.4%), whereas CR was observed in only 30.8% among WT mice (p=0.019). Echocardiography showed that ventricular remodeling was more deteriorated in KO mice ...
ABCF3 - ABCF3 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family F (GCN20), member 3 (ABCF3) available for purchase from OriGene - Your Gene Company.
Purpose : The retina specific ATP binding cassette transporter A4 (ABCA4) is necessary for the clearance of all-trans-retinal from photoreceptor cells. Loss of this crucial function results in the accumulation of toxic bisretinoids, primarily N-retinylidene-N-retinylethanolamine (A2E). This ultimately leads to the Stargardt phenotype of increased autofluorescence and progressive RPE and photoreceptor cell loss. Adeno-associated virus (AAV) vectors have proven their utility for efficient gene transfer in the retina to a variety of cell types. However, the ABCA4 coding sequence (cds) of 6.8kb exceeds the payload capacity of a single AAV capsid of 4.8kb by far. AAV dual vectors have been shown to overcome this size restriction by splitting the cds between two vectors and packaging them in separate capsids. After co-infection of a cell the two vectored cDNAs recombine to reconstitute the full length cds. Here we present recent data on the effect of AAV dual vector mediated ABCA4 gene replacement ...
Division of Molecular Biotherapy [Y. I., M. N., K. K., S. T., E. I., Y. S.] and Division of Experimental Chemotherapy [T. T.], Cancer Chemotherapy Center, and Department of Molecular Diagnosis [Y. M.], Cancer Institute, Japanese Foundation for Cancer Research, Tokyo 170-8455, and Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113-0032 [T. T.], ...
Synthesis of carbon-11-labeled tariquidar derivatives as new PET agents for imaging of breast cancer resistance protein (ABCG2). Appl Radiat Isot. 2010 Jun; 68(6):1098-103 ...
Cell Biol Int. 2021 Mar 24. doi: 10.1002/cbin.11598. Online ahead of print.. ABSTRACT. Overexpression of breast cancer resistance protein (BCRP) plays a crucial role in the acquired multidrug resistance (MDR) in breast cancer. The elucidation of molecular events that confer BCRP-mediated MDR is of major therapeutic importance in breast cancer. Epithelial cell adhesion molecule (EpCAM) has been implicated in tumor progression and drug resistance in various types of cancers, including breast cancer. However, the role of EpCAM in BCRP-mediated MDR in breast cancer remains unknown. In the present study, we revealed that EpCAM expression was upregulated in BCRP-overexpressing breast cancer MCF-7/MX cells, and EpCAM knockdown using siRNA reduced BCRP expression and increased the sensitivity of MCF-7/MX cells to mitoxantrone (MX). Epithelial-mesenchymal transition (EMT) promoted BCRP-mediated MDR in breast cancer cells, and EpCAM knockdown partially suppressed EMT progression in MCF-7/MX cells. In ...
hypothetical protein, ABCB1LB, ATP-binding cassette, sub-family B (MDR/TAP), member 1-like B, A306_07528, ABC16, ABC member 16, MDR/TAP subfamily, AS27_06659, AS28_00614, ATP-binding cassette protein B11, ATP-binding cassette, sub-family B (MDR/TAP), member 11, ATP-binding cassette, subfamily B (MDR/TAP), member 11, ATP-binding cassette, sub-family B (MDR/TAP), member 11-like protein, ATP-binding cassette sub-family B member 11, ATP-binding cassette, sub-family B, member 11, bile salt export pump, BRIC2, BSEP, BSEP/SPGP, CB1_000638007, D623_10034923, GW7_06212, H920_16172, I79_001236, Lith1, liver bile salt export pump, M91_01875, M959_07155, MDA_GLEAN10024246, Multidrug resistance protein 1, N301_03105, N302_06788, N303_07198, N305_06591, N306_04080, N307_07545, N308_11810, N309_07944, N312_11735, N321_13718, N327_01303, N328_07355, N329_09470, N331_01374, N332_02914, N333_01536, N334_13094, N336_04014, N340_01262, N341_10800, PAL_GLEAN10025937, PFIC2, PFIC-2, PGY4, progressive familial ...
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specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C3 (ABCC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C3 (ABCC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C3 (ABCC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter C3 (ABCC3) in the samples is then determined by comparing the ...
The ATP-binding cassette transporter A1 (ABCA1) mediates the efflux of cellular unesterified cholesterol and phospholipid to lipid-poor apolipoprotein A-I. Chymase, a protease secreted by mast cells, selectively cleaves pre-β-migrating particles from high density lipoprotein (HDL)3 and reduces the efflux of cholesterol from macrophages. To evaluate whether this effect is the result of reduction of ABCA1-dependent or -independent pathways of cholesterol efflux, in this study we examined the efflux of cholesterol to preparations of chymase-treated HDL3 in two types of cell: 1) in J774 murine macrophages endogenously expressing low levels of scavenger receptor class B, type I (SR-BI), and high levels of ABCA1 upon treatment with cAMP; and 2) in Fu5AH rat hepatoma cells endogenously expressing high levels of the SR-BI and low levels of ABCA1. Treatment of HDL3 with the human chymase resulted in rapid depletion of pre-β-HDL and a concomitant decrease in the efflux of cholesterol and phospholipid ...
ABCA12 - ABCA12 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family A (ABC1), member 12 (ABCA12), transcript variant 2 available for purchase from OriGene - Your Gene Company.
Cholesterol is an essential molecule that mediates a myriad of critical cellular processes, such as signal transduction in eukaryotes, membrane fluidity, and steroidogenesis. As such it is not surprising that cholesterol homeostasis is tightly regulated, striking a precise balance between endogenous synthesis and regulated uptake/efflux to and from extracellular acceptors. In mammalian cells, sterol efflux is a key component of the homeostatic equation and is mediated by members of the ATP binding cassette (ABC) transporter superfamily. ATP-binding cassette (ABC) transporters represent a group of evolutionarily highly conserved cellular transmembrane proteins that mediate the ATP-dependent translocation of substrates across membranes. Members of this superfamily, ABCA1 and ABCG1, are key components of the reverse cholesterol transport pathway. ABCG1 acts in concert with ABCA1 to maximize the removal of excess cholesterol from cells by promoting cholesterol efflux onto mature and nascent HDL particles
S. griseus mutant NP4, which was isolated by UV mutagenesis, showed a bald and wrinkled colony morphology because of ectopic septation in substrate hyphae and subsequent spore formation. The ectopic spores were the same as aerial spores in size, thickness of the spore wall, and shape, as determined by transmission and scanning electron microscopy, and in heat and lysozyme susceptibility. Mutant NP4 also formed abundant spores in liquid medium, whereas the parental strain IFO13350 rarely forms submerged spores under these conditions. The wall of the ectopic spores is supposed to be thicker than those of the submerged spores formed by several Streptomyces spp., including S. griseus B-2682 (32), under specific conditions, because the spores of NP4 were resistant to lysozyme. We therefore assume that both on solid and in liquid medium, mutant NP4 forms two separate cross walls in the vegetative hyphae and matures each compartment into a spore indistinguishable from aerial spores in many aspects, as ...
Active drug efflux by the adenosine triphosphate-binding cassette (ABC) transporter ABCG2 is one of the common mechanisms causing multiple drug resistance in various human cancers. In the intrinsic drug resistance of hepatocellular carcinoma (HCC), the role of ABCG2 is closely associated with side population (SP), a minor subset of cancer stem-like cells with unique capacity to extrude lipophilic dye Hoechst 33342 and many chemotherapeutic agents. In this study, we showed that ABCG2 was intrinsically expressed in a subgroup of HCC tissues and its expression pattern significantly influenced the levels of drug efflux from HCC cell lines. In MHCC-97L HCC cell line with intrinsic ABCG2 expression, we confirmed the importance of SP cells to the drug efflux-related chemotherapy resistance and found that the SP analysis provided an efficient method to evaluate the functional activity of ABCG2 transporter. In this cell line, we discovered that the SP proportion was modulated by the treatments of Akt ...
ATP binding cassette (ABC) transporters mediate vital transport processes in every living cell. ATP hydrolysis, which fuels transport, displays positive cooperativity in numerous ABC transporters. In particular, heterodimeric ABC exporters exhibit pronounced allosteric coupling between a catalytically impaired degenerate site, where nucleotides bind tightly, and a consensus site, at which ATP is hydrolyzed in every transport cycle. Whereas the functional phenomenon of cooperativity is well described, its structural basis remains poorly understood. Here, we present the apo structure of the heterodimeric ABC exporter TM287/288 and compare it to the previously solved structure with adenosine 5-(β,γ-imido)triphosphate (AMP-PNP) bound at the degenerate site. In contrast to other ABC exporter structures, the nucleotide binding domains (NBDs) of TM287/288 remain in molecular contact even in the absence of nucleotides, and the arrangement of the transmembrane domains (TMDs) is not influenced by ...
Mouse Monoclonal Anti-ABCD3 Antibody against Human ATP-binding cassette, sub-family D (ALD), member 3. Validated for Immunofluorescence and Immunohistochemistry
Our previous work shows that the stem cell factor SALL4 plays a central role in embryonic and leukemic stem cells. In this study, we report that SALL4 expression was higher in drug resistant primary acute myeloid leukemic patients than those from drug-responsive cases. In addition, while overexpression of SALL4 led to drug resistance in cell lines, cells with decreased SALL4 expression were more sensitive to drug treatments than the parental cells. This led to our investigation of the implication of SALL4 in drug resistance and its role in side population (SP) cancer stem cells. SALL4 expression was higher in SP cells compared to non-SP cells by 2-4 fold in various malignant hematopoietic cell lines. Knocking down of SALL4 in isolated SP cells resulted in a reduction of SP cells, indicating that SALL4 is required for their self-renewal. The SP phenotype is known to be mediated by members of the ATP-binding cassette (ABC) drug transport protein family, such as ABCG2 and ABCA3. Using ...
The ATP-sensitive K+ channel (KATP channel) couples glucose metabolism to insulin secretion in pancreatic beta-cells. It is comprised of sulfonylurea receptor (SUR)-1 and Kir6.2 proteins. Binding of Mg nucleotides to the nucleotide-binding domains (NBDs) of SUR1 stimulates channel opening and leads to membrane hyperpolarization and inhibition of insulin secretion. To elucidate the structural basis of this regulation, we constructed a molecular model of the NBDs of SUR1, based on the crystal structures of mammalian proteins that belong to the same family of ATP-binding cassette transporter proteins. This model is a dimer in which there are two nucleotide-binding sites, each of which contains residues from NBD1 as well as from NBD2. It makes the novel prediction that residue D860 in NBD1 helps coordinate Mg nucleotides at site 2. We tested this prediction experimentally and found that, unlike wild-type channels, channels containing the SUR1-D860A mutation were not activated by MgADP in either the presence
Transporter associated with antigen processing (TAP) is a member of the ATP-binding-cassette transporter family. It delivers cytosolic peptides into the endoplasmic reticulum (ER), where they bind to nascent MHC class I molecules. The TAP structure is formed of two proteins: TAP-1 and TAP-2, which have one hydrophobic region and one ATP-binding region each. They assemble into a heterodimer, which results in a four-domain transporter. The TAP transporter is found in the ER lumen associated with the peptide-loading complex (PLC). This complex of β2 microglobulin, calreticulin, ERp57, TAP, tapasin, and MHC class I acts to keep hold of MHC molecules until they have been fully loaded with peptides. TAP-mediated peptide transport is a multistep process. The peptide-binding pocket is formed by TAP-1 and TAP-2. Association with TAP is an ATP-independent event, in a fast bimolecular association step, peptide binds to TAP, followed by a slow isomerisation of the TAP complex. It is suggested that the ...
d-Methionine is an effective methionine source for Escherichia coli (5, 11, 14). The transport system reported to take up d-methionine in E. coli is encoded by the metD locus (11, 12). The system was found to be energized by ATP and regulated by the level of the internal methionine pool (10, 11, 13). The metD locus was mapped between the fhuA (previously called tonA) and the proA loci (12). The specific genes involved in d-methionine transport have not yet been reported.. We have identified the abc-yaeE-yaeC gene cluster (now renamed metNIQ genes) as a likely candidate for the metD locus in the fhuA-proA region. The abc gene was previously found in a search for ABC transporter ATP-binding domains (1). The PROSITE program (6) indicated that the Abc protein harbors an ATP- and GTP-binding site motif A (P-loop) (24) and an ABC transporter family signature (4). Gene yaeE encodes a putative membrane protein with a high sequence similarity to several bacterial amino acid transporters and contains a ...
Human Transporter Database: Comprehensive Knowledge and Discovery Tools in the Human Transporter Genes. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
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TY - JOUR. T1 - Drug efflux by breast cancer resistance protein is a mechanism of resistance to the benzimidazole insulin-like growth factor receptor/insulin receptor inhibitor, BMS-536924. AU - Hou, Xiaonan. AU - Huang, Fei. AU - Carboni, Joan M.. AU - Flatten, Karen. AU - Asmann, Yan. AU - Ten Eyck, Cynthia. AU - Nakanishi, Takeo. AU - Tibodeau, Jennifer D.. AU - Ross, Douglas D.. AU - Gottardis, Marco M.. AU - Erlichman, Charles. AU - Kaufmann, Scott H. AU - Haluska, Paul. PY - 2011/1. Y1 - 2011/1. N2 - Preclinical investigations have identified insulin-like growth factor (IGF) signaling as a key mechanism for cancer growth and resistance to clinically useful therapies in multiple tumor types including breast cancer. Thus, agents targeting and blocking IGF signaling have promise in the treatment of solid tumors. To identify possible mechanisms of resistance to blocking the IGF pathway, we generated a cell line that was resistant to the IGF-1R/InsR benzimidazole inhibitors, BMS-554417 and ...
GF120918 has a high inhibitory effect on P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP). We developed [11C]GF120918 as a positron emissi
Active drug efflux transporters of the ATP binding cassette (ABC)-containing family of proteins have a major impact on the pharmacological behavior of most of the drugs in use today. Pharmacological properties affected by ABC transporters include the oral bioavailability, hepatobiliary, direct intes …
Background: Development of a multidrug resistance (MDR) phenotype to chemotherapy remains a major barrier in the treatment of cancer. Gankyrin (p28, p28GANK or PSMD10) is an oncoprotein overexpressed in different carcinoma cell lines. The aim of this study was to compare Gankyrin expression level in MDR cells (MCF-7/ADR and MCF-7/ MX) and non-MDR counterparts (MCF-7). Methods: Gankyrin, MDR1 (also known as ABCB1; the ATP-binding cassette sub-family B member 1) and ABCG2 (also known as BCRP; the human breast cancer resistance protein) mRNA levels were analyzed by real-time RT-PCR. Western blot analysis was used to detect the protein expression levels of Gankyrin. Results: The PCR results showed that the expression of Gankyrin was significantly lower in the ABCG2 overexpressing cell line MCF-7/MX than in non-resistanct MCF-7 cells. In contrast, there were no significant differences in mRNA expression of Gankyrin in the MDR1 overexpressing cell line MCF-7/ADR in comparison with MCF-7 cells. Similarly,
OR10G8 (olfactory receptor family 10 subfamily G member 8), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
1. Jacquemin E. Progres-sive familial intrahepatic cholestasis. Clin Res Hepatol Gastroenterol 2012; 36 (Suppl 1): S26- S35. doi: 10.1016/ S2210-7401(12)70018-9. 2. Strautnieks SS, Bull LN, Knisely AS et al. A gene encod-ing a liver-specific ABC transporter is mutated in progres-sive familial intrahepatic cholestasis. Nat Genet 1998; 20(3): 233- 238. 3. Jansen PL, Mül-ler M. The molecular genetics of familial intrahepatic cholestasis. Gut 2000; 47(1): 1- 5. 4. Gerloff T, Stieger B, Hagenbuch B et al. The sister of P-glycoprotein represents thecanalicular bile salt export pump of mam-malian liver. J Biol Chem 1998; 273(16): 10046- 10050. 5. Davis RA, Miyake JH, Hui TY et al. Regulation of cholesterol-7alpha-hydroxylase: BAREly mis-s-ing SHP. J Lipid Res 2002; 43(4): 533- 543. 6. Thompson R, Strautnieks SS. BSEP: function and role in progres-sive familial intrahepatic cholestasis. Semin Liver Dis 2001; 21(4): 545- 550. 7. Kaliciński PJ, Ismail H, Jankowska I et al. Surgical treatment of ...
TY - JOUR. T1 - Mutations in the canalicular multispecific organic anion transporter (cMOAT) gene, a novel ABC transporter, in patients with hyperbilirubinemia II/Dubin-Johnson syndrome. AU - Wada, Morimasa. AU - Toh, Satoshi. AU - Taniguchi, Ken. AU - Nakamura, Takanori. AU - Uchiumi, Takeshi. AU - Kohno, Kimitoshi. AU - Yoshida, Ichiro. AU - Kimura, Akihiko. AU - Sakisaka, Shotaro. AU - Adachi, Yukihiko. AU - Kuwano, Michihiko. PY - 1998/2. Y1 - 1998/2. N2 - Members of the ATP-binding cassette (ABC) transporter superfamily are mutated to cause diseases that include cystic fibrosis, hyperinsulinemia, adrenoleukodystrophy, Stargardt disease and multidrug resistance. We recently isolated a novel human member of ABC transporter superfamily as the candidate transporter for the glucuronide and glutathione-conjugated antitumor agents, and found it highly homologous to the rat cmoat gene. Consistent with recent findings of defects in the homologous cmoat gene in two rat models of hyperbilirubinemia ...
Progressive familial intrahepatic cholestasis (PFIC) comprises a group of rare cholestatic liver disorders of childhood that could lead to liver cirrhosis. Nowadays, the partial biliary diversion procedure is still a therapeutic option in non-cirrhotic children with PFIC1 or PFIC2 after an ineffective ursodeoxycholic acid (UDCA) therapy. However, the relevant disadvantage of the partial external biliary diversion (PEBD) is that adolescent patients could not accept a permanent stoma. In some of them, despite of good clinical and biochemical results of this procedure, the ileal exclusion (IE) procedure had to be performed many years after PEBD. Our aims were to find the most characteristic early microscopic features of the disease as well as to compare changes in the liver biopsy specimens at the time of diagnosis and long-time (more than 10 years) after a surgical procedure. We examined retrospectively 8 liver biopsies from 4 PFIC2 patients comparing the results from the first biopsies done at ...
Breast cancer resistance protein (BCRP, ABCG2) is a member of ABC (ATP-binding cassette) transporter superfamily that occurs in a variety of tissues including liver and small intestine of animals. As BCRP is involved in drug absorption, distribution, and elimination, modulation of its expression may affect the clinical efficacy of drugs. However, little is known about the effects of coccidiosis or colibacillosis infection on the levels of BCRP expression in chickens. Here, we studied the effect of infection with Escherichia coli (E. coli) or Eimeriida mixture (E. necatrix and E. tenella) on the expression levels of ABCG2 mRNA and BCRP in the different segments of small intestine and liver in chickens. Expression of ABCG2 mRNA or BCRP was detected in the entire small intestine and liver of healthy chickens, and the expression levels in liver and ileum were significantly higher than duodenum and jejunum. Infection with E. coli or Eimeriida mixture resulted in significant decrease in ABCG2 mRNA and ...
Swiss drugmakers Roche and Novartis have provided financial backing French gene therapy start-up Vivet Therapeutics, with the latter raising EUR37.5 million (US$41 million) in an initial financing round.. The funds will be used by Vivet to advance a diversified pipeline of gene therapy programs targeting rare, inherited metabolic diseases, including Wilson Disease, progressive familial intrahepatic cholestasis type 2 (PFIC2), progressive familial intrahepatic cholestasis type 3 (PFIC3) and citrullinemia type I.. Vivet, created last year in Paris with a wholly owned subsidiary in Spain, is focused on developing novel gene therapies for rare, inherited metabolic diseases.. Its lead program VTX801, which is expected to enter clinical testing by the end of 2018, targets a condition called Wilson Disease.. This rare genetic disorder is caused by a defective gene in liver cells encoding the ATP7B protein, which reduces the livers ability to regulate copper levels in the liver and other tissues ...
Looking for online definition of ATP-binding cassette, sub-family B (MDR/TAP), member 10 in the Medical Dictionary? ATP-binding cassette, sub-family B (MDR/TAP), member 10 explanation free. What is ATP-binding cassette, sub-family B (MDR/TAP), member 10? Meaning of ATP-binding cassette, sub-family B (MDR/TAP), member 10 medical term. What does ATP-binding cassette, sub-family B (MDR/TAP), member 10 mean?
Some Candida albicans isolates from AIDS patients with oropharyngeal candidiasis are becoming resistant to the azole antifungal agent fluconazole after prolonged treatment with this compound. Most of the C. albicans isolates resistant to fluconazole fail to accumulate this antifungal agent, and this has been considered a cause of resistance. This phenomenon was shown to be linked to an increase in the amounts of mRNA of a C. albicans ABC (ATP-binding cassette) transporter gene called CDR1 and of a gene conferring benomyl resistance (BENr), the product of which belongs to the class of major facilitator multidrug efflux transporters (D. Sanglard, K. Kuchler, F. Ischer, J. L. Pagani, M. Monod, and J. Bille, Antimicrob. Agents Chemother. 39:2378-2386, 1995). To analyze the roles of these multidrug transporters in the efflux of azole antifungal agents, we constructed C. albicans mutants with single and double deletion mutations of the corresponding genes. The mutants were tested for their ...
Purpose: : To investigate the progression in Stargardt-fundus flavimaculatus (S-FFM). Methods: : A longitudinal study of 31 patients with S-FFM was undertaken. The mean age of our patients at the initial examination was 28.8 years old (11-50) with a mean follow-up interval of 9.3 years (5-11). Electrophysiological tests, including pattern, focal and full-field electroretinogram (ERG) were performed, as was a clinical ophthalmic examination and fundus autofluorescence imaging. The classification which we previously reported was used for assessment; group 1: dysfunction confined to the macula; group 2: macular and generalized cone dysfunction; group 3: macular, generalized cone and generalized rod dysfunction. At the initial examination, there were 13 patients in group 1, 8 patients in group 2, and 10 patients in group 3. Analysis of the ATP-binding cassette transporter gene (ABCA4) was performed in the majority of the cohort. Results: : Three of 13 patients from group 1 progressed to group 2, and ...
Retinoic acids regulate the reverse cholesterol transport by inducing the ATP binding cassette transporter A1 (ABCA1) dependent cholesterol efflux in macrophages, neuronal as well as intestine cells. In the present study, we aim to test the effect of all trans retinoic acid (ATRA) on ABCA1 expression in human CD4+ T cells and the involvement of cholesterol in ATRA mediated anti-HIV effect. Treatment with ATRA dramatically up-regulated ABCA1 expression in CD4+ T cells in a time and dose dependent manner. The expression of ABCA1 paralleled with increased ABCA1-dependent cholesterol efflux. This induction was dependent on T cell receptor (TCR) signaling and ATRA failed to induce ABCA1 expression in resting T cells. Moreover, ATRA and liver X receptor (LXR) agonist-TO-901317 together had synergistic effect on ABCA1 expression as well as cholesterol efflux. Increased ABCA1 expression was associated with lower cellular cholesterol staining. Cells treated with either ATRA or TO-901317 were less vulnerable to
Because little is known about ABC transporter functions in insects, we examined these proteins in T. castaneum, a well-established and powerful genomic insect model that is highly susceptible to systemic RNAi [22]. Bioinformatic analyses revealed that the ABC transporter superfamily comprises 73 genes in T. castaneum, which group into eight subfamilies. With 35 members, subfamily C constitutes the largest ABC transporter subfamily in T. castaneum. Compared with ABCC subfamilies in other insects, TcABCC appears to have undergone a recent expansion on chromosome 5. In insects, ABC transporters have been implicated in insecticide resistance by increasing the efflux capacity for xenobiotics [37]. This suggests the possibility that the known propensity of this species to develop insecticide resistance might be related to the expansion of subfamily C genes. Indeed, extensive forms of gene duplication (or amplification) are frequently observed for genes involved in insecticide detoxification, including ...
Oxidized sterols consumed in the diet or formed on low-density lipoprotein (LDL) are toxic to endothelial cells and macrophages and are thought to have a central role in promoting atherogenesis. The ATP-binding cassette transporter ABCG1 was recently shown to promote efflux of cholesterol from macrophages to HDL. We show that HDL protects macrophages from apoptosis induced by loading with free cholesterol or oxidized LDL (oxLDL). The protective effect of HDL was reduced in Abcg1−/− macrophages, especially after loading with oxidized LDL. Similarly, HDL exerted a protective effect against apoptosis induced by 7-ketocholesterol, the major oxysterol present in oxLDL and atherosclerotic lesions, in Abcg1+/+ but not in Abcg1−/− macrophages. In transfected 293 cells, efflux of 7-ketocholesterol was completely dependent on expression of ABCG1 and presence of HDL in media. In contrast, ABCA1 and apoA-1 did not stimulate efflux of 7-ketocholesterol into media. HDL stimulated efflux of ...
Differential Effects of Chrysin on Nitrofurantoin Pharmacokinetics Mediated by Intestinal Breast Cancer Resistance Protein in Rats and Mice
The LXRs (liver X receptors) (LXRα and LXRβ) are nuclear hormone receptors that are activated by oxysterols, endogenous oxidative metabolites of cholesterol. These receptors regulate an integrated network of genes that control whole body cholesterol and lipid homoeostasis. A brief overview of the mechanism of this regulation by LXRs in the liver, macrophage and intestine will be outlined, followed by data from our recent work demonstrating that LXRα is crucial in maintaining adrenal cholesterol homoeostasis. In the adrenal gland, oxysterols are formed as intermediates in the conversion of cholesterol into steroid hormones and can act as endogenous activators of LXR. We have found using both gain- and loss-of-function models that LXR acts to maintain free cholesterol below toxic levels in the adrenal gland, through the co-ordinated regulation of genes involved in cholesterol efflux [ABCA1 (ATP-binding-cassette transporter A1)], storage (sterol-regulatory-element-binding protein-1c and ...
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein is likely involved in the peroxisomal transport or catabolism of very long chain fatty acids. Defects in the human gene have been identified as the underlying cause of adrenoleukodystrophy, an X-chromosome recessively inherited demyelinating disorder of the nervous system. [provided by RefSeq, Jul 2008 ...
P-glycoprotein (P-gp) is a member of the ATP-Binding Cassette transporter superfamily and mediates transmembrane efflux of many drugs. Since it is involved in multi-drug resistance activity in various cancer cells, the development of P-gp inhibitor is one of the major concerns in anticancer therapy. Human P-gp protein has at least two functional drug binding sites that are called H site and R site, hence it has multi-binding-specificities. Though the amino acid residues that constitute in drug binding pockets have been proposed by previous experimental evidences, the shapes and the binding poses are not revealed clearly yet. In this study, human P-gp structure was built by homology modeling with available crystal structure of mouse P-gp as a template and docking simulations were performed with inhibitors such as verapamil, hoechst33342, and rhodamine123 to construct the interaction between human P-gp and its inhibitors. The docking simulations were performed 500 times for each inhibitor, and
In progressive familial intrahepatic cholestasis type 2 (PFIC-2), severe steatorrhea is often documented. However, pancreatic exocrine secretion has not yet bee
From NCBI Gene:. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein likely plays an important role in peroxisome biogenesis. Mutations have been associated with some forms of Zellweger syndrome, a heterogeneous group of peroxisome assembly disorders. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]. From UniProt: ...
The EGFR pathway has been an attractive target because it is dysregulated in a significant fraction of malignant gliomas through overexpression, amplification, and activating mutations (Rich et al., 2004). Moreover, recent studies have demonstrated that EGFRvIII is required for tumor maintenance in glioma (Mukasa et al., 2010). The EGFR tyrosine kinase inhibitor gefitinib has been evaluated in a number of clinical trials for GBM; however, results have been disappointing (Rich et al., 2004; Lieberman et al., 2004). The failure of gefitinib raises questions pertaining to delivery of drug to its target. Active efflux at the BBB could prevent drugs from attaining therapeutic levels in the brain and is probably one of the main reasons behind resistance to chemotherapy. It has been shown that several other tyrosine kinase inhibitors are avid substrates for P-gp and BCRP and that their brain distribution is limited due to active efflux out of the brain (Dai et al., 2003; Chen et al., 2009; Lagas et ...
The Original One-Hour Scripted Drama, Which Launched as the Highest-Rated Original Series Debut in the Networks History in Early June, Continues to Grow In Ratings Throughout Its First Three Episodes. Burbank, CA (June 28, 2010) - ABC Family has ordered 12 additional episodes of the networks hit one-hour series, Pretty Little Liars, announced Kate Juergens, Executive Vice President, Original Programming & Development. The order will bring the shows first season to a total of 22 episodes.. Pretty Little Liars is a break-out hit for the network, said Juergens. Were thrilled that our Millennial viewers have responded so strongly to the show. Its a perfect fit with the networks strong slate of original scripted dramas, including The Secret Life of the American Teenager and Make It or Break It.. One of cables biggest breakout hits of summer, Pretty Little Liars launched as ABC Familys No. 1 series debut ever in Adults 18-34 (837,000), Women 18-34 (657,000), Viewers 12-34 (1.7 ...
Phospho-glycoprotein (P-gP) is a polytopic plasma membrane protein whose overexpression causes multidrug resistance (MDR) responsible for the failure of cancer chemotherapy. P-gp 170 is a member of the ATP-binding cassette (ABC) transporter superfamily and has two potentially interesting regions for drugs interfering with its efflux function, namely the oligosaccharides on the first extracellular loop with unknown function and the two intracellular ATP-binding regions providing the energy for drug efflux function. The polylactoseamine oligosaccharides on the first loop can specifically bind the tomato lectin (TL). The P-gp efflux activities of TL-pre-treated MDR resistant cells were measured in the presence of structurally unrelated resistance modifiers such as phenothiazines, terpenoids and carotenoids. The inhibition of efflux activity was measured via the increased rhodamine uptake by mouse lymphoma cells transfected in human MDR1 gene and in human brain capillary endothelial cells. The ...
The overlap in the membrane localization and substrate specificities of Bcrp and P-gp has suggested their cooperation in the active efflux in the blood-tissue barriers. A considerable increase in the accumulation of common substrates in the brain of Mdr1a/1b(−/−)/Bcrp(−/−) mice compared with that observed in either Mdr1a/1b(−/−) or Bcrp(−/−) mice has been interpreted as synergistic effect. The present study investigated this synergistic effect kinetically by using erlotinib, flavopiridol, and mitoxantrone as test compounds. Because Cisternino et al. (2004) reported an induction of Bcrp mRNA in the brain capillaries from Mdr1a/1b(−/−) mice, quinidine and dantrolene were used as probes for P-gp and Bcrp, respectively, to examine their adaptive regulation in the knockout strain.. Adaptive regulation of P-gp at the BBB and BTB of Bcrp(−/−) mice is negligible because neither the Cbrain/Cplasma nor the Ctestis/Cplasma of quinidine changed in Bcrp(−/−) mice compared with the ...
In this study, we performed a mutation analysis of the ABCR gene of 80 unrelated AMD patients and 100 race and age matched control subjects. Of 51 exons of the ABCR gene, 10 that have been reported to have AMD associated mutations were studied.3 The only missense mutation found was a T1428M mutation in exon 29. Allikmetset al reported that this mutation was found in one of 167 AMD patients, and that no such mutation was found in the general population.3 However, in our series, seven of 80 (8.8%) AMD patients and eight of 100 (8.0%) of the control group did, in fact, showed this mutation (Table 1), which was not a statistically significant difference. Other alterations found were mostly polymorphisms or sequence variations. These were found in exons 23, 41, 43, and did not correspond to the 13 AMD associated mutations reported by Allikmets et al.3Apparently, there is heterogeneity in the ABCR gene. Indeed, all of the AMD and the control subjects in our series showed different nucleotide sequences ...
The ATP-binding cassette transporters P-glycoprotein (ABCB1 MDR1) and multidrug resistance protein 4 (MRP4) efflux irinotecan and its active metabolite SN-38 genotype was a significant covariate for the clearance of both irinotecan Epigallocatechin gallate lactone and SN-38 Epigallocatechin gallate lactone. of the topoisomerase Epigallocatechin gallate I enzyme [2]. SN-38 undergoes glucuronic acid Rabbit polyclonal to TranscriptionfactorSp1. … Continue reading The ATP-binding cassette transporters P-glycoprotein (ABCB1 MDR1) and multidrug resistance protein. ...
AUTOSOMAL RECESSIVE STARGARDT DISEASE (STGD1) is the most common inherited juvenile macular degeneration.1 Most patients develop bilateral loss of vision in childhood or early adulthood. This subtype of Stargardt disease is caused by mutations in the ABCA4 gene, which encodes a retina-specific transporter protein (ABCR) in the rims of rod and cone photoreceptor outer segment discs.2-4 Retinal degeneration in ABCA4-linked Stargardt disease is believed to result from the toxic effects of lipofuscin that accumulates in the retinal pigment epithelium (RPE) and the subsequent degeneration of photoreceptors.5. Light can induce photochemical injury at the ocular fundus. Depending on the level and duration of the irradiance, the primary site of damage can be either the photoreceptors or the RPE.6 In ABCA4-linked retinopathies, products generated by the visual cycle accumulate and contribute to retinal damage via both direct toxic effects and increased photosensitivity. A major fluorophore of lipofuscin, ...
ATP-sensitive potassium (KATP) channels comprise four pore-forming Kir6.2 subunits and four modulatory sulfonylurea receptor (SUR) subunits. The latter belong to the ATP-binding cassette family of transporters. KATP channels are inhibited by ATP (or ADP) binding to Kir6.2 and activated by Mg-nucleotide interactions with SUR. This dual regulation enables the KATP channel to couple the metabolic state of a cell to its electrical excitability and is crucial for the KATP channels role in regulating insulin secretion, cardiac and neuronal excitability, and vascular tone. Here, we review the regulation of the KATP channel by adenine nucleotides and present an equilibrium allosteric model for nucleotide activation and inhibition. The model can account for many experimental observations in the literature and provides testable predictions for future experiments.
Introduction Multidrug resistance (MDR) is a major cause of treatment failure and mortality in cancer patients. Breast cancer is the most prevalent cancer among women and the second leading cause of death in cancer. The most widely used treatment of breast cancer is chemotherapy, while the success of chemotherapy in breast cancer patients is also seriously limited by the development of MDR [1]. One well-known mechanism of MDR is the over-expression of ATP-binding cassette transporters such as multidrug resistance gene 1 (MDR1), multidrug resistance-associated protein 1 (MRP1), Sunitinib lung resistance protein (LRP). and the breast cancer resistance protein (BCRP) [2-7]. P-glycoprotein (P-gp), which is encoded by the MDR1, is the most extensively studied drug transporter. It is an integral membrane glycoprotein with a molecular mass of 170 kDa and has been postulated to function as a pump that removes hydrophobic anticancer agents from drug-resistant cells, thus promoting MDR [8]. The novel gene ...
TY - JOUR. T1 - No association of ABCB1 polymorphisms with drug-refractory epilepsy in a north Indian population. AU - Lakhan, R.. AU - Misra, U. K.. AU - Kalita, J.. AU - Pradhan, S.. AU - Gogtay, N. J.. AU - Singh, M. K.. AU - Mittal, B.. N1 - Funding Information: The study was supported by a grant received from the Department of Biotechnology, Government of India, and a fellowship provided by CSIR, New Delhi, India.. PY - 2009/1. Y1 - 2009/1. N2 - Multiple drug resistance is a common problem in the treatment of epilepsy, and approximately 30% of patients continue to have seizures despite all therapeutic interventions. Among various classes of drug transporters, genetic variants of P-glycoprotein (P-gp) encoded by the ABCB1 (ATP-binding cassette subfamily B member 1) gene have been associated with drug-refractory epilepsy. Our aim was to investigate the effect of the 1236C,T(rs1128503), 2677G,T/A(rs2032582), and 3435C,T(rs1045642) single-nucleotide polymorphisms of ABCB1 (or MDR1) on drug ...
In the study of motor proteins, the molecular mechanism of mechanochemical coupling, as well as the cellular role of these proteins, is an important issue. To assess these questions we introduced cDNA of wild-type and site-directed mutant kinesin heavy chains into fibroblasts, and analyzed the behavior of the recombinant proteins and the mechanisms involved in organelle transports. Overexpression of wild-type kinesin significantly promoted elongation of cellular processes. Wild-type kinesin accumulated at the tips of the long processes, whereas the kinesin mutants, which contained either a T93N- or T93I mutation in the ATP-binding motif, tightly bound to microtubules in the center of the cells. These mutant kinesins could bind to microtubules in vitro, but could not dissociate from them even in the presence of ATP, and did not support microtubule motility in vitro, thereby indicating rigor-type mutations. Retrograde transport from the Golgi apparatus to the endoplasmic reticulum, as well as ...
Mutations in the ATP8B1 gene can cause benign recurrent intrahepatic cholestasis 1 (BRIC1) or progressive familial intrahepatic cholestasis 1 (PFIC1), commonly known as ATP8B1 deficiency. ATP8B1 is an aminophospholipid flippase, maintaining membrane asymmetry. In ATP8B1 deficiency the activity of the bile salt export pump (BSEP) which pumps bile salts out ... read more of the hepatocytes into the bile duct is decreased, leading to accumulation of bile salts and cell damage. However, the mechanism by which ATP8B1 defects causes cholestasis is unknown. Involvement of the nuclear receptor FXR or reduced membrane stability and consequent decreased BSEP activity have been suggested. Proper ATP8B1 folding and association with CDC50A is required for ATP8B1 to exit the ER and traffic to the plasma membrane. It was recently demonstrated that ATP8B1 mutations often result in protein misfolding and subsequent ER retention and protein degradation. Molecular chaperones facilitate protein folding and ...
The clearance of apoptotic cells occurs throughout the lifespan of multicellular organisms and is important for development during embryogenesis, the maintenance of tissue integrity and function, and the resolution of inflammation (deCathelineau and Henson, 2003). Here, we report that macrophage ABCA7 enhances the clearance of apoptotic cells in vitro and in vivo. ABCA7 and LRP1 move to the cell surface after stimulation with C1q or apoptotic cells and localize to membrane ruffles or phagocytic cups, respectively. However, ABCA7 also localizes to phagocytic membranes during FcR-mediated phagocytosis, in which ABCA7 levels are not rate limiting. More important, ABCA7 is required for optimal ligand-induced signaling through LRP1, as shown by C1q-induced ERK phosphorylation and for sustained ERK phosphorylation during phagocytosis of apoptotic cells. Finally, ERK phosphorylation itself is shown to be essential for phagocytosis of apoptotic cells but not for FcR-mediated phagocytosis, suggesting a ...
Introduction: The breast cancer resistance protein BCRP, encoded by ABCG2, is a member of the ABC transporter family and is well-known for its contribution to multi-drug resistance in cancer. Although BCRP expression is altered in various cancer types, its role and function in cancer, independent from drug efflux, remains largely elusive. In this study, we aimed to elucidate regulatory mechanisms of ABCG2 expression and its biological relevance in clear cell renal cell carcinoma (ccRCC) as well as associations with response to sunitinib treatment in patients with metastatic ccRCC.. Experimental Procedures: Data from the ccRCC cohort of The Cancer Genome Atlas (TCGA) was used to assess ABCG2 mRNA expression (n=463), DNA methylation (n=288) and genetic variants present in tumor tissue (n=326) within the ABCG2 gene region. An independent ccRCC cohort of 64 patients was used for the analysis of ABCG2 mRNA and protein expression by TaqMan quantitative real time PCR or immunohistochemical staining of ...
ABC transporters play a key role in protecting the brain parenchyma by exerting their action at the blood-brain barrier (BBB). However, they also block the entry of therapeutic drugs. One of the key transporters playing this role is ABCG2. While other ABC transporters can be studied through PET, no probe exists for imaging ABCG2 function. D-luciferin, the endogenous substrate of fLuc, has been shown to demonstrate decreased bioluminescence in ABCG2-expressing cells, and low brain distribution. We hypothesized that we can image ABCG2 function at the BBB using bioluminescent imaging in transgenic mice expressing fLuc in the brain. In vitro, accumulation of D-luciferin was lowest in cells overexpressing ABCG2, and the accumulation increased when co-administered Ko143, a potent and selective ABCG2 inhibitor. Using a mouse model expressing fLuc behind the GFAP promoter, mainly expressed in astrocytes of the brain, D-luciferin BLI signal was found to be low. However, it increased in a dose-dependent ...
Background. Our aim was to determine whether the common variants within the coding sequence of ABCA1 gene affects low plasma high-density lipoprotein cholesterol (HDL-C) levels in Turkish patients with coronary artery disease (CAD). The study group was composed of 552 CAD patients, of which 251 had HDL-C levels 40 mg/dL. ...
The bile salt export pump (BSEP) is an ABC-transporter expressed at the canalicular membrane of hepatocytes. Its physiological role is to expel bile salts into the canaliculi from where they drain into the bile duct. Inhibition of this transporter may lead to intrahepatic cholestasis. Predictive computational models of BSEP inhibition may allow for fast identification of potentially harmful compounds in large databases. This article presents a predictive in silico model based on physicochemical descriptors that is able to flag compounds as potential BSEP inhibitors. This model was built using a training set of 670 compounds with available BSEP inhibition potencies. It successfully predicted BSEP inhibition for two independent test sets and was in a further step used for a virtual screening experiment. After in vitro testing of selected candidates, a marketed drug, bromocriptin, was identified for the first time as BSEP inhibitor. This demonstrates the usefulness of the model to identify new BSEP ...
BmrA from Bacillus subtilis is a half-size ABC (ATP-binding cassette) transporter involved in multidrug resistance. Although its supramolecular organization has been investigated after reconstitution in a lipid bilayer environment, and shows a dimeric and possibly a tetrameric form, the precise quaternary structure in a detergent-solubilized state has never been addressed. In the present study, BmrA was purified from Escherichia coli membranes using an optimized purification protocol and different detergents. Furthermore, the ATPase activity of BmrA and the quantity of bound lipids and detergent were determined, and the oligomeric state was analysed using SEC (size-exclusion chromatography) and analytical ultracentrifugation. The activity and the quaternary structure of BmrA appeared to be strongly influenced by the type and concentration of the detergent used. SEC data showed that BmrA could be purified in a functional form in 0.05 and 0.01% DDM (n-dodecyl-β-D-maltoside) and was homogeneous ...
The ATP-binding cassette (ABC) transporter ABCB1, encoded by the multidrug resistance gene MDR1, is expressed on brain microvascular endothelium and several types of epithelium, but not on endothelia outside the CNS. It is an essential component of the blood-brain barrier. The aim of this study was to identify cell-specific controls on the transcription of MDR1 in human brain endothelium. Reporter assays identified a region of 500bp around the transcription start site that was optimally active in brain endothelium. Chromatin immunoprecipitation identified Sp3 and TFIID associated with this region and EMSA (electrophoretic mobility shift assays) confirmed that Sp3 binds preferentially to an Sp-target site (GC-box) on the MDR1 promoter in brain endothelium. This result contrasts with findings in other cell types and with the colon carcinoma line Caco-2, in which Sp1 preferentially associates with the MDR1 promoter. Differences in MDR1 transcriptional control between brain endothelium and Caco-2 ...
The ATP-binding cassette (ABC) transporters form one of the largest known protein families, and are widespread in bacteria, archaea, and eukaryotes. They couple ATP hydrolysis to active transport of a wide variety of substrates such as ions, sugars, lipids, sterols, peptides, proteins, and drugs. The structure of a prokaryotic ABC transporter usually consists of three components; typically two integral membrane proteins each having six transmembrane segments, two peripheral proteins that bind and hydrolyze ATP, and a periplasmic (or lipoprotein) substrate-binding protein. Many of the genes for the three components form operons as in fact observed in many bacterial and archaeal genomes. On the other hand, in a typical eukaryotic ABC transporter, the membrane spanning protein and the ATP-binding protein are fused, forming a multi-domain protein with the membrane-spanning domain (MSD) and the nucleotide-binding domain (NBD ...
Expression of the multidrug transporter P-glycoprotein (P-gp) is one of the major causes of multidrug resistance in cancer cells. P-gp is a 170-kDa membrane protein encoded by the MDR1 gene in humans. Based on its sequence and domain organization, P-gp is classified as a member of ATP binding cassette superfamily; it consists of two symmetrical halves, each half containing six transmembrane (TM) domains and a cytoplasmic nucleotide binding domain. Although most members of the ABC transporter family have stringent substrate specificities, P-gp recognizes many compounds, including anthracyclines (e.g., doxorubicin and daunomycin), vinca alkaloids (e.g., vincristine and vinblastine), antibiotics (e.g., actinomycin D), circular and toxic peptides (e.g., valinomycin and gramicidin), and relatively noncytotoxic agents such as verapamil, azidopine, quinidine, and cyclosporin A. These P-gp substrates have no common chemical structure. They are all low-molecular-weight nonanionic hydrophobic or ...
EXCLUSIVE: What if Sex and the Citys Carrie Bradshaw was dying of cancer? That in essence is the premise of the Mexican drama series Terminales, which is being adapted by ABC Family. Lionsgate TV and Kapital Entertainment are producing the U.S.
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Predicted to have ATPase-coupled transmembrane transporter activity and lipid transporter activity. Involved in neutral lipid metabolic process. Localizes to endoplasmic reticulum. Orthologous to human ABCA17P (ATP binding cassette subfamily A member 17, pseudogene ...
This work is the first description of the expression of human BCRP in X. laevis oocytes and demonstrates that this heterologous expression system is a valid model for examining the physical and functional characteristics of this ABC half transporter. X. laevis oocytes injected with BCRP cRNA synthesize a protein with the molecular mass of glycosylated BCRP and the immunological characteristics of native human BCRP protein. Our results further demonstrate that oocytes injected with mutant R482T or wild-type BCRP cRNA express BCRP in the oocyte plasma membrane, as evidenced by confocal immunofluorescence microscopic analysis. Accumulation and efflux assays using various BCRP substrate molecules indicate that the oocyte-expressed BCRP functions in a manner analogous to that observed in mammalian systems. Moreover, the X. laevis model indicates that a mutation of serine at codon 187 in the ABC-signature motif of BCRP is not only devoid of transporter activity but also serves in a manner analogous to ...
Among the various transporter genes identified in the C. albicans genome (7, 8), there are overwhelming clinical and experimental evidences to suggest that out of 28 ABC transporters, only two of its members, CaCdr1p and CaCdr2p, and only CaMdr1p out of a total 95 of members of MFS transporters are major determinants of MDR (18, 23). Therefore, the search for novel and potent modulators which can block and reverse the drug extrusion mediated by these efflux proteins represents an attractive strategy of anti-Candida therapy. While there are several compounds which are shown to reverse MDR mediated by Pgp (human homologue of CaCdr1p and CaCdr2p) and are at various stages of clinical trials, there are not many instances of compounds which could inhibit/reverse drug extrusion mediated by fungal multidrug transporters (12, 14, 22, 31). We have been exploring modulators/inhibitors of MDR pump proteins which could block the efflux of drugs from fungal cells. For example, we have earlier reported that ...
One of the main obstacles in tumor therapy is multiple drug resistance (MDR) and an underlying mechanism of MDR is up-regulation of the transmembrane ATP-binding cassette (ABC) transporter proteins, especially P-glycoprotein (P-gp). In the synergistic treatment of siRNA and anti-cancer drug doxorubicin, it is crucial that both the siRNA and doxorubicin are simultaneously delivered to the tumor cel ...
ABCB10, or ATP binding cassette sub-family B member 10, is a protein localized in the mitochondrial inner membrane. It belongs to the ABC transporter family whose members are proteins that facilitate substrate transport across various biological membranes. It has been found that ABCB10 is required for normal heme biosynthesis during erythroid differentiation and also plays a role in protection against the damage caused by reactive oxygen species (ROS) production. This protective effect exists both in the erythrocyte development and in the heart recovery after the ischemia-reperfusion injury. However, as an ABC transporter, its transported substrates are not known, neither is the mechanism by which ABCB10 plays a role in protection against ROS damage. In this dissertation an 8-azido-ATP photolabeling system is established to study the ATP binding and hydrolysis properties of ABCB10. Using this approach, it is found that the conserved amino acid residues Gly497 and Lys498 in the Walker A motif of ...
This year, Shawnee Town will celebrate the iconic 1920s with our new series, History on Tap, bringing together presentations on subjects that the decade is most readily associated with - sex, gangsters, bootlegging, and baseball. A different local brewery will be on hand each of the four History on Tap evenings this year to showcase their wares. Short skirts, shorter hair, revealing lingerie, and loose morals are what most of us associate with the decade of the flapper. Was this the norm or that of a small group of pampered young women flouting their angst at Mama? Come and find out at our first History on Tap!. Price: 5. ...
Rea is known for his work on vacuolar proton (H+) pumps, ATP-binding cassette (ABC) transporters, and the enzyme phytochelatin ... Rea, P.A. (2007). "Plant ATP-binding cassette transporters". Annual Review of Plant Biology. 58: 347-375. doi:10.1146/annurev. ... an Arabidopsis ATP-binding cassette transporter able to transport glutathione S-conjugates and chlorophyll catabolites: ... an Arabidopsis ATP-binding cassette transporter able to transport glutathione S-conjugates and chlorophyll catabolites: ...
ATP-binding cassette transporter GRCh38: Ensembl release 89: ENSG00000172350 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... The protein encoded by this gene is included in the ATP-binding cassette transporter (ABC protein) superfamily. ABC proteins ... Dean M, Rzhetsky A, Allikmets R (Jul 2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Res. 11 (7 ... 2002). "Molecular and cytogenetic characterization of the mouse ATP-binding cassette transporter Abcg4". Gene. 293 (1-2): 67-75 ...
"Characterization and functional analysis of the nucleotide binding fold in human peroxisomal ATP binding cassette transporters ... "ATP binding/hydrolysis by and phosphorylation of peroxisomal ATP-binding cassette proteins PMP70 (ABCD3) and ... The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... Gärtner J, Valle D (1993). "The 70 kDa peroxisomal membrane protein: an ATP-binding cassette transporter protein involved in ...
ATP-binding cassette sub-family D member 2 is a membrane pump/transporter protein that in humans is encoded by the ABCD2 gene. ... ATP-binding cassette transporter ABCD2 has been shown to interact with PEX19. GRCh38: Ensembl release 89: ENSG00000173208 - ... The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... and heterodimerization of peroxisomal ATP-binding cassette half-transporters". J. Biol. Chem. 274 (46): 32738-43. doi:10.1074/ ...
Vasiliou V, Vasiliou K, Nebert DW (April 2009). "Human ATP-binding cassette (ABC) transporter family". Human Genomics. 3 (3): ... ATP-binding cassette sub-family A member 13 also known as ABCA13 is a protein that in humans is encoded by the ABCA13 gene on ... Articles with short description, Short description matches Wikidata, Genes on human chromosome 7, ATP-binding cassette ... "The human ATP binding cassette gene ABCA13, located on chromosome 7p12.3, encodes a 5058 amino acid protein with an ...
Löscher, Wolfgang; Potschka, Heidrun (2005-01-01). "Blood-brain barrier active efflux transporters: ATP-binding cassette gene ... Löscher, Wolfgang; Potschka, Heidrun (2005-05-01). "Role of drug efflux transporters in the brain for drug disposition and ... "Drug resistance in brain diseases and the role of drug efflux transporters". Nature Reviews Neuroscience. 6 (8): 591-602. doi: ...
ATP-binding cassette transporter ABCA1 (member 1 of human transporter sub-family ABCA), also known as the cholesterol efflux ... The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters ... ATP-binding cassette transporter GRCh38: Ensembl release 89: ENSG00000165029 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... August 1999). "The gene encoding ATP-binding cassette transporter 1 is mutated in Tangier disease". Nature Genetics. 22 (4): ...
This gene is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two ... ABCA9 ATP-binding cassette, sub-family A (ABC1), member 9". Dean M, Rzhetsky A, Allikmets R (2001). "The human ATP-binding ... ATP-binding cassette sub-family A member 9 is a protein that in humans is encoded by the ABCA9 gene. ... cassette (ABC) transporter superfamily". Genome Res. 11 (7): 1156-66. doi:10.1101/gr.184901. PMID 11435397. S2CID 9528197. ...
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... ABCF2 acts as a suppressor of the volume-sensitive outwardly rectifying Cl channel (CLCN3). ATP-binding cassette transporter ... Dean M, Rzhetsky A, Allikmets R (July 2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Research. ... ATP-binding cassette transporters, All stub articles, Membrane protein stubs). ...
The protein belongs to the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules ... Dean, Michael (2002-11-01). "The Human ATP-Binding Cassette (ABC) Transporter Superfamily". National Library of Medicine (US), ... ATP binds at the cytoplasmic side of the protein. Following binding of each, ATP hydrolysis shifts the substrate into a ... ADP is released, and a new molecule of ATP binds to the secondary ATP-binding site. Hydrolysis and release of ADP and a ...
ATP-binding cassette transporter sub-family C member 11, also MRP8 (Multidrug Resistance-Related Protein 8) is a membrane ... ATP-binding cassette transporter Body odor GRCh38: Ensembl release 89: ENSG00000121270 - Ensembl, May 2017 "Human PubMed ... Dean M, Rzhetsky A, Allikmets R (Jul 2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Research. 11 ... The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ...
ATP-binding cassette sub-family A member 12 also known as ATP-binding cassette transporter 12 is a protein that in humans is ... Dean M, Rzhetsky A, Allikmets R (2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Res. 11 (7): ... "Entrez Gene: ATP-binding cassette". Y. Ishibashi; A. Kohyama-Koganeya; Y. Hirabayashi (2013). "New insights on glucosylated ... ABCA12 belongs to a group of genes called the ATP-binding cassette family, which makes proteins that transport molecules across ...
This gene is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two ATP- ... Dean M, Rzhetsky A, Allikmets R (Jul 2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Res. 11 (7 ... "Two new genes from the human ATP-binding cassette transporter superfamily, ABCC11 and ABCC12, tandemly duplicated on chromosome ... "Multiple splicing variants of two new human ATP-binding cassette transporters, ABCC11 and ABCC12". Biochem. Biophys. Res. ...
ATP Binding Cassette or efflux transporters mediate the transport of substrates across cell membranes against a concentration ... Articles with short description, Short description matches Wikidata, ATP-binding cassette transporters). ... The activity of the multidrug transporter in drug-resistant cells is associated with rapid cellular ATP depletion when ATP ... ATP cleavage is tightly linked to substrate translocation, as the energy for the substrate translocation is derived from ATP ...
"Membrane topology distinguishes a subfamily of the ATP-binding cassette (ABC) transporters". FEBS Lett. 402 (1): 1-3. doi: ... In enzymology, a channel-conductance-controlling ATPase (EC 3.6.3.49) is an enzyme that catalyzes the chemical reaction ATP + ... The systematic name of this enzyme class is ATP phosphohydrolase (channel-conductance-controlling). As of late 2007, two ... H2O ⇌ {\displaystyle \rightleftharpoons } ADP + phosphate Thus, the two substrates of this enzyme are ATP and H2O, whereas its ...
One example is ATP-binding cassette (ABC) transporter families. P-glycoproteins (PGPs) are part of this family and substrates ... In general, drug resistance can develop in four different ways: 1) the drug does not bind to the target due to target changes, ... MLs bind irreversible to Glutmate gated chloride ion (GluCl) channels, leading to hyperpolarisation. Pharyngeal and somatic ... This includes transporters which are part of the xenobiotic metabolism by absorbing, distributing and eliminating external ...
MRP2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across ... ATP-binding cassette transporter GRCh38: Ensembl release 89: ENSG00000023839 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... "Entrez Gene: ABCC2 ATP-binding cassette, sub-family C (CFTR/MRP), member 2". Sekine T, Miyazaki H, Endou H (February 2006). " ... or ATP-binding cassette sub-family C member 2 (ABCC2) is a protein that in humans is encoded by the ABCC2 gene. ...
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... Kajinami K, Brousseau ME, Nartsupha C, Ordovas JM, Schaefer EJ (Apr 2004). "ATP binding cassette transporter G5 and G8 ... "Mutations in the human ATP-binding cassette transporters ABCG5 and ABCG8 in sitosterolemia". Human Mutation. 20 (2): 151. doi: ... "Entrez Gene: ABCG5 ATP-binding cassette, sub-family G (WHITE), member 5 (sterolin 1)". Schmitz G, Langmann T, Heimerl S (Oct ...
It induces the expression of ATP-binding cassette transporter, which can export toxic substances out of the cell, allowing ... Both Pdr1p and Pdr3p regulate Pdr5p, which is an ATP-binding cassette transporter. A single amino acid substitution mutation, ... Pdr1p binds to sequence 5'-TCCGYGGR-3' of PDRE, which is located within the promoter sequences of its target genes. 218 genes ... It binds to DNA sequences that contain certain motifs called pleiotropic drug response element (PDRE). Pdr1p is encoded by a ...
Lr34 encodes an adenosine triphosphate (ATP)-binding cassette (ABC) transporter. The dominant allele that provides disease ... The xa13 gene has a mutated effector-binding element in its promoter that eliminates PthXo1 binding and renders these lines ... Examples: Auxin: binds to receptors that then recruit and degrade repressors of transcriptional activators that stimulate auxin ... Many R genes encode NB-LRR proteins (proteins with nucleotide-binding and leucine-rich repeat domains, also known as NLR ...
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... membrane-spanning domain that sets it apart from other transporters within the ATP-binding cassette family of transporters. The ... ATP-binding cassette transporter P-glycoprotein (Multidrug resistance protein- MDR1). Not to be confused with Multidrug ... ATP-binding Cassette (ABC) Transporter". Journal of Biological Chemistry. 289 (45): 30880-30888. doi:10.1074/jbc.R114.609248. ...
Petry, Frauke (2004). Charakterisierung eines neuen ATP-binding-cassette Transporters aus der ABCA-Subfamilie (PDF) (in German ...
2004). "ATP binding cassette transporter G5 and G8 genotypes and plasma lipoprotein levels before and after treatment with ... The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... 2002). "Mutations in the human ATP-binding cassette transporters ABCG5 and ABCG8 in sitosterolemia". Hum. Mutat. 20 (2): 151. ... 2002). "Catalog of 605 single-nucleotide polymorphisms (SNPs) among 13 genes encoding human ATP-binding cassette transporters: ...
ATP binding cassette (ABC) type transporters are common to the three domains of life. Some secreted proteins are translocated ... The process begins as a leader sequence on the protein to be secreted is recognized by HlyA and binds HlyB on the membrane. ... Thanassi DG, Stathopoulos C, Karkal A, Li H (2005). "Protein secretion in the absence of ATP: the autotransporter, two-partner ... This signal sequence is extremely specific for the ABC transporter. The HlyAB complex stimulates HlyD which begins to uncoil ...
Putative ATP-binding cassette transporter sub-family C member 13 is a protein that is not present in humans. In humans, ABCC13 ... ATP-binding cassette transporter ABCC13+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) ... This gene is a member of the superfamily of genes encoding ATP-binding cassette (ABC) transporters. ABC proteins transport ... Dean M, Annilo T (2005). "Evolution of the ATP-binding cassette (ABC) transporter superfamily in vertebrates". Annual Review of ...
Her research focuses on elucidating the structure and function of ATP-binding cassette (ABC) transporters. Chen was born in ... where she started studying the ATP binding cassette transporters In 2002, Chen became an assistant professor at Purdue ... "A Tweezers-like Motion of the ATP-Binding Cassette Dimer in an ABC Transport Cycle". Molecular Cell. 12 (3): 651-661. doi: ... Her work on ABC transporters includes investigating their role in resistance to chemotherapy drugs; antigen presentation in ...
"A Plant Plasma Membrane ATP Binding Cassette-Type Transporter Is Involved in Antifungal Terpenoid Secretion". Plant Cell. 13 (5 ...
October 2003). "ATP-binding cassette transporter A7 (ABCA7) binds apolipoprotein A-I and mediates cellular phospholipid but not ... August 2006). "ATP-binding cassette transporter A7 enhances phagocytosis of apoptotic cells and associated ERK signaling in ... August 2006). "ATP-binding cassette transporter A7 enhances phagocytosis of apoptotic cells and associated ERK signaling in ... January 2017). "ATP Binding Cassette Transporter ABCA7 Regulates NKT Cell Development and Function by Controlling CD1d ...
"The ATP/Substrate Stoichiometry of the ATP-binding Cassette (ABC) Transporter OpuA". Journal of Biological Chemistry. Retrieved ... He is an expert in the field of ATP-binding cassette transporters, one of the largest known protein families, by combining ... Van Der Heide, Tiemen; Poolman, Bert (2002). "ABC transporters: one, two or four extracytoplasmic substrate‐binding sites?". ... His group was the first to show that changes in the ionic strength are used to gate the activity of osmoregulatory transporters ...
Buechler C, Boettcher A, Bared SM, Probst MC, Schmitz G (2002). "The carboxyterminus of the ATP-binding cassette transporter A1 ... "The carboxyterminus of the ATP-binding cassette transporter A1 interacts with a beta2-syntrophin/utrophin complex". Biochem. ... and each bind to dystrophin and its relatives". J Biol Chem. 271 (5): 2724-30. doi:10.1074/jbc.271.5.2724. PMID 8576247. Ahn AH ... "The receptor tyrosine phosphatase-like protein ICA512 binds the PDZ domains of beta2-syntrophin and nNOS in pancreatic beta- ...
ATP binding cassette), na katerih poteče razpad molekule ATP, ta pa omogoči črpanje raznih ionov.[19] Poleg tega se preko ... "Plant ABC Transporters". The Arabidopsis Book / American Society of Plant Biologists. Vol. 9. doi:10.1199/tab.0153. ISSN 1543- ... Opisan tip F-črpalke je tako imenovana ATP-sintaza, ker katalizira nastajanje energijsko bogatih molekul ATP.[19] Podobna F- ... Črpalke ali prenašalci, ki aktivni transport izvajajo, tako da pri tem porabljajo energijo ATP, so denimo P-črpalke (ki se ...
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters ... 2002). "Catalog of 605 single-nucleotide polymorphisms (SNPs) among 13 genes encoding human ATP-binding cassette transporters: ... "Entrez Gene: ABCA8 ATP-binding cassette, sub-family A (ABC1), member 8". Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The ... ATP-binding cassette sub-family A member 8 is a protein that in humans is encoded by the ABCA8 gene. ...
Up to 19 types of permease exist to transport various carbohydrates with 13 being ATP-binding cassette transporters. B. longum ... The persistence of B. longum in the gut is attributed to the glycoprotein-binding fimbriae structures and bacterial ...
"Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9)". J ... The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters ... ATP-binding cassette transporter GRCh38: Ensembl release 89: ENSG00000150967 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... ATP-binding cassette transporters, All stub articles, Membrane protein stubs). ...
Following the synthesis, the ATP-binding cassette transporters (teichoic-acid-transporting ATPase) TarGH (P42953, P42954) flip ... A set of enzymes and transporters named DltABCE that adds alanines to both wall and lipo-teichoic acids were found. Note that ... whereas teichoic acids that are covalently bound to peptidoglycan are referred to as wall teichoic acids (WTA). The most common ...
Drug transporters like MXR1 and P-glycoprotein are part of the ATP-binding cassette transporter group. Hrycyna studies the ... The MDR1 gene results in expression of a P-glycoprotein and related drug transporter MXR1, which are involved in mitoxantrone ... the multi-drug transporter P-glycoprotein. Whilst it is understood that P-glycoproteins are important in the movement of ... and Pharmacological Aspects of the Multidrug Transporter". Annual Review of Pharmacology and Toxicology. 39 (1): 361-398. doi: ...
ATP-binding cassette transporters, Mutated genes, Cystic fibrosis, Chloride channels). ... ATP free), and one where it is outward facing (ATP bound). ATP binds to each nucleotide-binding domain, which results in the ... ATP binding, and the hydrolysis that follows, drives the alternative exposure of the cargo binding site, ensuring a ... ABC transporters have evolved to transduce the free energy of ATP hydrolysis to the uphill movement of substrates across the ...
ERβ function is related to various cardiovascular targets including ATP-binding cassette transporter A1 (ABCA1) and ... The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus ... "The crystal structure of the estrogen receptor DNA-binding domain bound to DNA: how receptors discriminate between their ... Upon binding to 17-β-estradiol, estriol or related ligands, the encoded protein forms homo-dimers or hetero-dimers with ...
To overcome this difficulty, the ATP-binding cassette (ABC) transporter C1 (ABCC1) serves as the "exit door" for S1P. On the ... It binds rhodopsin, a well-characterized GPCR that binds all-cis retinal in its inactive state. Upon photoisomerization by a ... The binding of IP3 to IP3R releases Ca2+ from the ER into the normally Ca2+-poor cytoplasm, which then triggers various events ... DAG remains bound to the membrane by its fatty acid "tails" where it recruits and activates both conventional and novel members ...
The protein encoded by the ABCC6 gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... also known as ATP-binding cassette sub-family C member 6 (ABCC6) and multi-specific organic anion transporter E (MOAT-E) is a ... ATP-binding cassette transporters, All stub articles, Membrane protein stubs). ... "Entrez Gene: ABCC6 ATP-binding cassette, sub-family C (CFTR/MRP), member 6". O'Neill GM, Peters GB, Harvie RM, MacKenzie HB, ...
Mahe Y, Lemoine Y, Kuchler K (1996). "The ATP binding cassette transporters Pdr5 and Snq2 of Saccharomyces cerevisiae can ... is structurally related to the ATP-binding cassette superfamily". J. Bacteriol. 177 (6): 1536-43. doi:10.1128/jb.177.6.1536- ... "The Transporter Factsbook, Academic Press, San Diego, 1998". {{cite journal}}: Cite journal requires ,journal= (help) Prasad R ... In enzymology, a xenobiotic-transporting ATPase (EC 3.6.3.44) is an enzyme that catalyzes the chemical reaction ATP + H2O + ...
... from cells by interaction with the ATP-binding cassette transporter A1 (ABCA1). A plasma enzyme called lecithin-cholesterol ... "The target of regulating the ATP-binding cassette A1 protein (ABCA1): promoting ABCA1-mediated cholesterol efflux in different ... such as by interaction with the ABCG1 transporter and the phospholipid transport protein (PLTP). HDL transports cholesterol ...
Genes under the control of malT include ATP-binding cassette transporter components, maltoporin, maltose binding protein, and ... MalT binds to maltose or maltodextrin and undergoes a conformational change that allows it to bind DNA at sequences near the ...
... which is a membrane transporter from the large ATP-binding cassette transporter family. The protein is expressed in most organs ... ABCC6 mediates ATP release in the liver. This is the main source of circulating pyrophosphate (PPi), and individuals affected ...
ATP-binding cassette transporter ABCB8+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) ... The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters ... ATP-binding cassette transporters, All stub articles, Membrane protein stubs). ... "Entrez Gene: ABCB8 ATP-binding cassette, sub-family B (MDR/TAP), member 8". Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). " ...
Other possible targets include glycerol kinase and substrate binding proteins in ATP-binding cassette transporters (ABC ... This CU-rich region is suggested to be the functional region that binds the ribosome-binding site (RBS) of mRNAs. While the ... it has been suggested that tsRNAs regulate gene expression by binding the ribosome binding sequence of multiple mRNAs. The low ... transporters). Therefore, many evidences suggest that tsRNAs regulate carbohydrate and amino acid metabolism, however, the ...
Reverses Multidrug Resistance by Inhibiting the Efflux Function of Multiple ATP-Binding Cassette Transporters". Cancer Research ...
"Purification of ATP-binding cassette transporter A1 and associated binding proteins reveals the importance of beta1-syntrophin ...
ABC transporter transmembrane domain is the main transmembrane structural unit of ATP-binding cassette transporter proteins, ... "Structure and association of ATP-binding cassette transporter nucleotide-binding domains". Biochim. Biophys. Acta. 1561 (1): 47 ... ATP-binding cassette transporters, Protein domains, Protein families, All stub articles, Membrane protein stubs). ... structures of the MJ1267 ATP binding cassette reveal an induced-fit effect at the ATPase active site of an ABC transporter". ...
... presence of alterations in gene expressions that are involved in energy metabolism such as ATP-binding cassette transporters. ... Iron is the site of oxygen binding for hemoglobin, without it, hemoglobin is unable to bind to oxygen and transport it to the ...
ATP-binding cassette transporter Taylor NM, Manolaridis I, Jackson SM, Kowal J, Stahlberg H, Locher KP (June 2017). "Structure ... The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters ... Broad substrate specificity ATP-binding cassette transporter ABCG2) at the PDBe-KB. This article incorporates text from the ... ATP-binding cassette super-family G member 2 is a protein that in humans is encoded by the ABCG2 gene. ABCG2 has also been ...
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters ... Dean M, Rzhetsky A, Allikmets R (2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Research. 11 (7 ... "ATP-binding cassette transporter ABCB5 gene is expressed with variability in malignant melanoma". Actas Dermo-sifiliograficas. ... "Correlation of induction of ATP binding cassette transporter A5 (ABCA5) and ABCB1 mRNAs with differentiation state of human ...
ATP-binding cassette, subfamily A, members 5 and 8 ASXL2: Additional sex combs like 2, transcriptional regulator ATRAID: ... iron-regulated transporter), member 1 SMPD4: Sphingomyelin phosphodiesterase 4 SP140: encoding protein SP140 nuclear body ... ATP-binding cassette, subfamily A (ABC1), member 12 ACTR1B: encoding protein Beta-centractin AGXT: alanine-glyoxylate ... Rna binding motif protein 44 RFX8: Rfx family member 8, lacking rfx dna binding domain RIF1: replication timing regulatory ...
ATP-dependent association of the nucleotide binding cassettes during the catalytic cycle of ATP-binding cassette transporters ... The ATP-binding cassette transporters (ABC transporters) are a transport system superfamily that is one of the largest and ... Each member of the ABCF subgroup consist of a pair of ATP binding domains. Six half transporters with ATP binding sites on the ... Dimer formation of the two ABC domains of transporters requires ATP binding. It is generally observed that the ATP bound state ...
ATP-dependent association of the nucleotide binding cassettes during the catalytic cycle of ATP-binding cassette transporters ... The ATP-binding cassette transporters (ABC transporters) are a transport system superfamily that is one of the largest and ... Each member of the ABCF subgroup consist of a pair of ATP binding domains. Six half transporters with ATP binding sites on the ... Dimer formation of the two ABC domains of transporters requires ATP binding. It is generally observed that the ATP bound state ...
Antibiotic That Inhibits the ATPase Activity of an ATP-Binding Cassette Transporter by Binding to a Remote Extracellular Site. ... Antibiotic That Inhibits the ATPase Activity of an ATP-Binding Cassette Transporter by Binding to a Remote Extracellular Site. ... Antibiotic That Inhibits the ATPase Activity of an ATP-Binding Cassette Transporter by Binding to a Remote Extracellular Site. ... Antibiotic That Inhibits the ATPase Activity of an ATP-Binding Cassette Transporter by Binding to a Remote Extracellular Site. ...
ATP-Binding Cassette Transporter G8 Gene As a Determinant of Apolipoprotein B-100 Kinetics in Overweight Men. ... Dive into the research topics of ATP-Binding Cassette Transporter G8 Gene As a Determinant of Apolipoprotein B-100 Kinetics in ...
In this study, mechanisms that regulate mRNA or protein expression of ATP-binding cassette transporters were characterized in ... ATP binding cassette (ABC) transporters are a family of proteins whose activity is vital to cell detoxification, protection ... Finally, transcription factor regulators of ABC transporters were identified by searching for transcription factor binding ... In particular, the impact of such variation on the role of ABC transporters in transport of pharmaceutical agents is of ...
Mouse ABCG2(ATP Binding Cassette Transporter G2) ELISA Kit. Mouse ABCG2(ATP Binding Cassette Transporter G2) ELISA Kit ... ATP Binding Cassette Transporter G2 (ABCG2) Polyclonal Antibody (Mouse), APC-Cy7. 4-PAA960Mu01-APC-Cy7 Cloud-Clone * 538.00 EUR ... ATP Binding Cassette Transporter G2 (ABCG2) Polyclonal Antibody (Human), APC-Cy7. 4-PAA960Hu01-APC-Cy7 Cloud-Clone * 527.00 EUR ... Mouse ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit. SEA960Mu-10x96wellstestplate Cloud-Clone 10x96-wells test plate. ...
ATP-binding cassette transporters, ABCA1 and ABCG1, are cell surface transporters that mediate the reverse cholesterol ... Siripurkpong P, Na-Bangchang K. Effects of niacin and chromium on the expression of ATP-binding cassette transporter A1 and ... Liao H, Langmann T, Schmitz G, Zhu Y. Native LDL upregulation of ATP-binding cassette transporter 1 in human vascular ... Nicotinic acid, lauric acid and kaempferol abolish ATP-binding cassette transporter subfamily A member 1 (ABCA1) down- ...
Protein ATP-binding cassette sub-family A member 2, Mouse ATP-binding cassette sub-family A member 2 Protein, Mouse ATP-binding ... Protein ATP-binding cassette sub-family A member 2, Mouse ATP-binding cassette sub-family A member 2 Protein, Mouse ATP-binding ... ABC-A2, ABC2, ATP-binding cassette sub-family A member 2, ABC-A2, ABC2, ATP-binding cassette sub-family A member 2, ABC-A2 ... cassette sub-family A member 2, Mouse protein ATP-binding cassette sub-family A member 2, ABC-A2 protein, Protein ABC-A2, Mouse ...
T1 - CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 ... CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 ... CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 ... CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 ...
ABC: ATP-binding cassette transporter; apoB-LP: apoB containing lipoprotein; LXR: Liver X receptor; SRB1: Scavenger receptor B1 ... AMG145 is a human monoclonal IgG2 that binds specifically to PCSK9 The investigational medicinal product is given as 420 mg ...
The ABCG2 gene belongs to a group of genes called the ATP-binding cassette family; genes in this family provide instructions ... ATP-binding cassette transporter G2. *ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group) ... The ABCG2 gene belongs to a group of genes called the ATP-binding cassette family; genes in this family provide instructions ... multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2 ...
An ATP-binding cassette (ABC) transporter located in the inner mitochondrial membrane is involved in iron-sulfur cluster and ... N2 - An ATP-binding cassette (ABC) transporter located in the inner mitochondrial membrane is involved in iron-sulfur cluster ... AB - An ATP-binding cassette (ABC) transporter located in the inner mitochondrial membrane is involved in iron-sulfur cluster ... abstract = "An ATP-binding cassette (ABC) transporter located in the inner mitochondrial membrane is involved in iron-sulfur ...
Human ABCC6(ATP Binding Cassette Transporter C6) ELISA Kit. Human ABCC6(ATP Binding Cassette Transporter C6) ELISA Kit. Contact ... Description: A sandwich ELISA kit for detection of ATP Binding Cassette Transporter C6 from Human in samples from blood, serum ... Description: A sandwich ELISA kit for detection of ATP Binding Cassette Transporter C6 from Mouse in samples from blood, serum ... Description: A sandwich ELISA for quantitative measurement of Human ATP Binding Cassette Transporter G1 in samples from blood, ...
title = "ATP-binding cassette transporter G2 mediates the efflux of phototoxins on the luminal membrane of retinal capillary ... T1 - ATP-binding cassette transporter G2 mediates the efflux of phototoxins on the luminal membrane of retinal capillary ... ATP-binding cassette transporter G2 mediates the efflux of phototoxins on the luminal membrane of retinal capillary endothelial ... ATP-binding cassette transporter G2 mediates the efflux of phototoxins on the luminal membrane of retinal capillary endothelial ...
... transporter 1 and 2, ATP-binding cassette (TAP1, TAP2); 4 components of major histocompatibility complex (MHC) class II gene ...
Human ABCC3(ATP Binding Cassette Transporter C3) ELISA Kit. Human ABCC3(ATP Binding Cassette Transporter C3) ELISA Kit ... Description: A sandwich ELISA kit for detection of ATP Binding Cassette Transporter C3 from Human in samples from blood, serum ... ATP Binding Cassette Transporter C3 (ABCC3) Polyclonal Antibody (Human, Pig), APC-Cy7. ... Description: A sandwich ELISA for quantitative measurement of Human ATP Binding Cassette Transporter G1 in samples from blood, ...
keywords = "ATP-binding cassette transporter, Mass spectrometry, Mast cell, Prostaglandin, Transporter",. author = "Nobuaki ... Tanaka N, Kawai J, Hirasawa N, Mano N, Yamaguchi H. ATP-Binding Cassette Transporter C4 is a Prostaglandin D2 Exporter in HMC-1 ... ATP-Binding Cassette Transporter C4 is a Prostaglandin D2 Exporter in HMC-1 cells. / Tanaka, Nobuaki; Kawai, Junya; Hirasawa, ... Tanaka, N., Kawai, J., Hirasawa, N., Mano, N., & Yamaguchi, H. (2020). ATP-Binding Cassette Transporter C4 is a Prostaglandin ...
Human ABCC13(ATP Binding Cassette Transporter C13) ELISA Kit. Human ABCC13(ATP Binding Cassette Transporter C13) ELISA Kit ... Human ATP Binding Cassette Transporter C13 (ABCC13) ELISA Kit. SED633Hu-1x48wellstestplate Cloud-Clone 1x48-wells test plate. ... Human ATP Binding Cassette Transporter C13 (ABCC13) ELISA Kit. SED633Hu-1x96wellstestplate Cloud-Clone 1x96-wells test plate. ... Human ATP Binding Cassette Transporter C13 (ABCC13) ELISA Kit. SED633Hu-5x96wellstestplate Cloud-Clone 5x96-wells test plate. ...
HLA-E is an MHC class Ib molecule that binds nonamer peptides derived from the leader sequence of MHC class 1a molecules and is ... Cutting Edge: HLA-E Binds a Peptide Derived from the ATP-Binding Cassette Transporter Multidrug Resistance-Associated Protein 7 ... HLA-E Binds a Peptide Derived from the ATP-Binding Cassette Transporter Multidrug Resistance-Associated Protein 7 aSnd Inhibits ... derived from the human ATP-binding cassette (ABC)5 transporter protein, multidrug resistance-associated protein 7 (MRP7), also ...
ATP-Binding Cassette (ABC) transporters have been demonstrated to be involved in the general stress response. In previous ... Involvement of a putative ATP-Binding Cassette (ABC) involved in manganese transport in virulence of Listeria monocytogenes. ... involvement of ABC transporters in the stress response. Taken together, our findings suggest that ABC transporters may be used ... transporter genes. First, the invasion efficiency in host cells was measured using the HT-29 human cell line. Second, cell-to- ...
ATP-dependent efflux transporter of diverse lipophilic neutral or cationic compounds. Previously, we identified two positively ... ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism* * Allosteric Site * Animals ... ATP-dependent efflux transporter of diverse lipophilic neutral or cationic compounds. Previously, we identified two positively ... Evidence for a third drug-binding site Eur J Biochem. 1999 Feb;259(3):841-50. doi: 10.1046/j.1432-1327.1999.00098.x. ...
Full-sized ATP-binding cassette (ABC) transporters of the G subfamily (ABCG) are considered to be essential components of the ... Full-sized ATP-binding cassette (ABC) transporters of the G subfamily (ABCG) are considered to be essential components of the ... Full-size ATP-binding cassette (ABC) transporters belonging to the ABCG subfamily are unique for plants and fungi. There is ... Plant genomes encode more than 100 ATP-binding cassette (ABC) transporters, a number far exceeding those of other organisms. ...
Chemotherapy-induced resistance by ATP-binding cassette transporter genes.. *J. Gillet, T. Efferth, J. Remacle ...
N2 - The ATP-binding cassette transporter G1 (ABCG1) was recently identified as a regulator of macrophage cholesterol and ... AB - The ATP-binding cassette transporter G1 (ABCG1) was recently identified as a regulator of macrophage cholesterol and ... The ATP-binding cassette transporter G1 (ABCG1) was recently identified as a regulator of macrophage cholesterol and ... abstract = "The ATP-binding cassette transporter G1 (ABCG1) was recently identified as a regulator of macrophage cholesterol ...
ATP Binding Cassette Transporter 1 * ATP-Binding Cassette Transporters / physiology* * Apolipoproteins / physiology* ...
ATP-binding cassette transporter A1 (ABCA1) is a cholesterol transporter that transfers. August 26, 2018 11:50 am. \ Leave a ... ATP-binding cassette transporter A1 (ABCA1) is a cholesterol transporter that transfers surplus mobile cholesterol onto lipid- ...
Atypical roles for Campylobacter jejuni amino acid ATP binding cassette transporter components PaqP and PaqQ in bacterial ... Dive into the research topics of Atypical roles for Campylobacter jejuni amino acid ATP binding cassette transporter ...
Dean, M., Hamon, Y., and Chimini, G. (2001). The Human ATP-Binding Cassette (ABC) Transporter Superfamily. J. Lipid Res. 42 (7 ... Schneider, E., and Hunke, S. (1998). ATP-binding-cassette (ABC) Transport Systems: Functional and Structural Aspects of the ATP ... ABC Transporter). The ABC transporters transport various substrates including amino acids, peptides, and cellular metabolites ... In the present study, we found up-regulation of the gene encoding insulin-like growth factor binding protein 5 in the HS cows, ...
ATP-Binding Cassette Transporters. Mustacich DJ, Leonard SW, Patel NK, Traber MG. 2010. Alpha-tocopherol beta-oxidation ...
  • Most of the uptake systems also have an extracytoplasmic receptor, a solute binding protein. (wikipedia.org)
  • ABC transporters are considered to be an ABC superfamily based on the similarities of the sequence and organization of their ATP-binding cassette (ABC) domains, even though the integral membrane proteins appear to have evolved independently several times, and thus comprise different protein families. (wikipedia.org)
  • Transporters are extremely vital in cell survival such that they function as protein systems that counteract any undesirable change occurring in the cell. (wikipedia.org)
  • In this study, mechanisms that regulate mRNA or protein expression of ATP-binding cassette transporters were characterized in human tissues. (escholarship.org)
  • CCAAT/Enhancer-binding protein delta (CEBPD) is an inflammation-responsive transcription factor and is proposed to be oncogenic in the context of drug resistance, prompting us to clarify its role in TMZ-resistant GBM. (elsevier.com)
  • Accordingly, the protein levels of stemness transcription factors, namely, SRY-box transcription factor 2 (SOX2), octamer-binding transcription factor 4 (OCT4), NANOG, and ATP-binding cassette subfamily A member 1 (ABCA1), were increased in GSCs and TMZ-treated GBM cells. (elsevier.com)
  • We have used mass spectrometry to identify and sequence a novel peptide from HLA-E following heat shock, ALALVRMLI, derived from the transmembrane domain of the human ATP-binding cassette protein, multidrug resistance-associated protein, MRP7. (aai.org)
  • P-glycoprotein is a plasma membrane protein of mammalian cells that confers multidrug resistance by acting as a broad-specificity, ATP-dependent efflux transporter of diverse lipophilic neutral or cationic compounds. (nih.gov)
  • The TATA-less proximal promoter contains multiple Sp1 binding sites and a consensus sequence for sterol regulatory element binding protein. (lih.lu)
  • Additionally the ChoX-acetylcholine complex permitted a detailed structural comparison with the carbamylcholine-binding site of the acetylcholine-binding protein from the mollusc Lymnaea stagnalis. (rcsb.org)
  • This structure is only the second of its kind and raises the important question of how ATP-binding cassette transporters are capable of distinguishing liganded and unliganded-closed states of the binding protein. (rcsb.org)
  • The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. (nih.gov)
  • The two structures are similar and delineate an elongated protein with the two transmembrane domains (TMD) forming an outward facing conformation, extended and twisted exocytoplasmic domains (ECD), and closely opposed nucleotide binding domains. (nature.com)
  • Breast cancer resistance protein (BCRP/ABCG2) is a member of the ATP-binding cassette (ABC) transporter family. (ncl.edu.tw)
  • The ATP- binding cassette superfamily of transporters includes many biologically and clinically important proteins, such as the cystic fibrosis protein (CFTR), the human multidrug resistance protein (MDR), and the peptide transporter of the MHC complex. (the-scientist.com)
  • The small RbcS-like domains of the β-carboxysome structural protein, CcmM, bind RubisCO at a site distinct from that binding the RbcS subunit. (uoguelph.ca)
  • Journal Article] ATP-binding cassette transporter Al gene transcription is downregulated by activator protein 2alpha. (nii.ac.jp)
  • The combination of structural and kinetic data illustrates how different conformations of MRP1 are temporally linked and how substrate and ATP alter protein dynamics to achieve active transport. (elifesciences.org)
  • Multidrug resistance protein 1 (MRP1) is an ATP-binding cassette (ABC) transporter that harnesses the energy of ATP to extrude substrates from the cytosol to the extracellular space ( Cole, 2014a ). (elifesciences.org)
  • Protein-bound molecules, including drugs, are eliminated by proximal tubular secretion via a well-coordinated process of uptake by the tubular cells at the blood-facing basolateral site and secretion into the tubular lumen. (springer.com)
  • Protein-bound metabolites (in orange) are actively secreted by the proximal tubule epithelial cells. (springer.com)
  • The objective of this study is to predict and analyze genes in three such families: NBS-LRR (nucleotide-binding sites and leucine-rich repeats or NLR), START (Steroidogenic Acute Regulatory protein [STaR] related lipid-transfer) and ABC (ATP-Binding Cassette) transporter. (biomedcentral.com)
  • The ATP-binding cassette (ABC) transporters form one of the largest known protein families, and are widespread in bacteria, archaea, and eukaryotes. (genome.jp)
  • typically two integral membrane proteins each having six transmembrane segments, two peripheral proteins that bind and hydrolyze ATP, and a periplasmic (or lipoprotein) substrate-binding protein. (genome.jp)
  • On the other hand, in a typical eukaryotic ABC transporter, the membrane spanning protein and the ATP-binding protein are fused, forming a multi-domain protein with the membrane-spanning domain (MSD) and the nucleotide-binding domain (NBD). (genome.jp)
  • Synthetic derivatives of the siderophore petrobactin were immobilized on an agarose−streptavidin matrix and treated with the cell lysate of a Bacillus subtilis culture, which allowed the successful identification of the petrobactin binding protein FpiA. (uni-marburg.de)
  • Furthermore, the triscatecholate binding protein FeuA from B. subtilis was structurally and functionally characterized. (uni-marburg.de)
  • Noticeable was the nearly identical binding mode of the protein for the different substrates, which is attended by a tilting of the two domains of FeuA of about 20°, the greatest experimentally observed domain movement of a class III substrate binding protein so far. (uni-marburg.de)
  • As the intracellular sterol traffic in macrophages is in part regulated by oxysterol binding protein (OSBP) and OSBP-related proteins (ORPs), we investigated the role of ORP11, localized at the Golgi-late endosomes interface, in the BMP-mediated protection from oxLDL/oxysterol cytotoxicity. (helsinki.fi)
  • Multidrug resistance-associated protein 1 (MRP-1) is a ubiquitously expressed member of the ATP-binding cassette transporter family. (ku.edu)
  • ATP-binding cassette (ABC) transporters form one of the largest groups of paralogous protein families that have a wide diversity of functions and substrates. (tocris.com)
  • Twenty-four hours later, we measured P-gp and BCRP protein expression in isolated brain capillary by immunoblotting as well as by transport activity in vivo by measuring the unbound drug partitioning coefficient of the brain (K p,uu,brain ) of known efflux transporter substrates administered intravenously. (biomedcentral.com)
  • Clumping factor A (ClfA) is the archetypal fibrinogen-binding surface protein of Staphylococcus aureus and a member of the microbial surface component recognizing adhesive matrix molecules (MSCRAMM) family. (microbiologyresearch.org)
  • Similarly, subdomain N1 of a related MSCRAMM fibronectin-binding protein B was required for export and surface display of the full-length protein, but not a derivative lacking fibronectin-binding repeats. (microbiologyresearch.org)
  • http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?list_uids=16636888&db=PubMed&dopt=Abstract&cmd=Retrieve] Palmieri G, Casbarra A, Fiume I, Catara G, Capasso A, Marino G, Onesti S, Rossi M. "Identification of the first archaeal oligopeptide-binding protein from the hyperthermophile Aeropyrum pernix. (kenyon.edu)
  • We show that this transport requires oxysterol-binding protein (OSBP)-related proteins (ORPs), which are a large family of conserved lipid-binding proteins. (rupress.org)
  • The enzyme, found in bacteria, interacts with an extracytoplasmic substrate binding protein and mediates the high-affinity import of molybdate and tungstate. (expasy.org)
  • There was a marked increase in gene expression of ABC transporters (MDR1 and MRPA) and protein phosphatase 2A that evince increased drug efflux. (itg.be)
  • amyloid beta precursor protein binding f. (gsea-msigdb.org)
  • damage specific DNA binding protein 2 [S. (gsea-msigdb.org)
  • diablo IAP-binding mitochondrial protein. (gsea-msigdb.org)
  • Here, we provide evidence that BfrAB regulates the expression of bfrCD and bfrEFG, which encode two ATP-binding cassette (ABC) transporters, and bfrH, which encodes a CAAX amino-terminal protease family protein. (elsevier.com)
  • The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. (gen-script.com)
  • This protein is thought to form ATP-sensitive potassium channels in cardiac, skeletal, and vascular and non-vascular smooth muscle. (gen-script.com)
  • Protein structure suggests a role as the drug-binding channel-modulating subunit of the extra-pancreatic ATP-sensitive potassium channels. (gen-script.com)
  • Despite the promising results of TKIs in clinical trials and clinical practise, recent in vitro studies suggested that their efficiency might be compromised due to the overexpression of the ATP-binding cassette (ABC) transporters, namely ABCB1 (P-glycoprotein, MDR1) and ABCG2 (breast cancer resistance protein, BCRP). (pensoft.net)
  • Exosomes are a class of bilayer-bound extracellular vesicles with a diameter of 30-100 nm, which contain various bioactive components such as protein, DNA, mRNA, miRNA, other non-coding RNA, lipid, and so forth. (biomedcentral.com)
  • The transporter specificity is determined by its cognate substrate-binding protein DppA, which captures ligands in the periplasm and delivers them to the permease. (bioinfor.com)
  • The loop that terminates the peptide-binding pocket in DppAs from other bacteria is significantly shorter in the H. pylori protein, providing an explanation for its ability to bind longer peptides. (bioinfor.com)
  • The subsites accommodating the two N-terminal residues of the peptide ligand make the greatest contribution to the protein-ligand binding energy, in agreement with the observation that dipeptides bind with affinity close to that of longer peptides. (bioinfor.com)
  • This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. (modellab.cn)
  • Molybdate pumping into the molybdenum storage protein via an ATP-powered piercing mechanism. (mpg.de)
  • The Molybdenum Storage Protein: A soluble ATP hydrolysis‐dependent molybdate pump. (mpg.de)
  • ABC transporters often consist of multiple subunits, one or two of which are transmembrane proteins and one or two of which are membrane-associated AAA ATPases. (wikipedia.org)
  • The third subgroup of ABC proteins do not function as transporters, but are rather involved in translation and DNA repair processes. (wikipedia.org)
  • Pathogens use siderophores, such as Enterobactin, to scavenge iron that is in complex with high-affinity iron-binding proteins or erythrocytes. (wikipedia.org)
  • ATP binding cassette (ABC) transporters are a family of proteins whose activity is vital to cell detoxification, protection against xenobiotics and oxidative stress, and maintenance of homeostasis of endogenous compounds. (escholarship.org)
  • Knowledge about plant ATP-binding cassette (ABC) proteins is of great value for sustainable agriculture, economic yield, and the generation of high-quality products, especially under unfavorable growth conditions. (researchgate.net)
  • This transporter together with ABCA1 belongs to a group of sterol-sensitive ABC proteins which are induced by lipid loading or specific oxysterols. (lih.lu)
  • These structural investigations revealed an architecture of the ChoX binding pocket and mode of substrate binding similar to that reported previously for several compatible solute-binding proteins. (rcsb.org)
  • Starting from native material or recombinant systems, we succeed with all types of membrane proteins: GPCRs, Ions Channels, Transporters, Receptors and Viral Proteins. (calixar.com)
  • CALIXAR's approach allows to preserve the original structure and function of membrane proteins (GPCRs, Ion Channels, Transporters, Receptors, Anchors and Viral Proteins) providing solutions for pharmaceutical industries, biotechnology companies and academic teams to develop conformational antibodies, formulate new vaccines, carry out Structure Based Drug Discovery and/or HTS assays. (calixar.com)
  • We report here that, in addition to the membranous form, ABCG2 proteins are also found inside the nucleus, where they bind to the E-box of CDH1 (E-cadherin) promoter and regulate transcription of this gene. (ncl.edu.tw)
  • A highly conserved ATP-binding domain is diagnostic of this superfamily of proteins. (the-scientist.com)
  • High affinity binding of siderophores is accomplished by substrate binding proteins, which are part of ATP-binding cassette transporters. (uni-marburg.de)
  • In this work an affinity chromatography-based approach for the isolation and identification of substrate binding proteins from crude cell extracts through direct interaction with its natural ligand has been established. (uni-marburg.de)
  • This work shows a new approach for the identification of bacterial substrate binding proteins and presents detailed structural and functional insights into the binding of triscatecholate siderophore by such proteins. (uni-marburg.de)
  • Many soluble proteins transit through the trans-Golgi network (TGN) and the prevacuolar compartment (PVC) en route to the vacuole, but our mechanistic understanding of this vectorial trafficking step in plants is limited. (researchgate.net)
  • Prokaryotic importers require additional extracytoplasmic binding proteins (one or more per systems) for function. (embl.de)
  • An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. (expasy.org)
  • We show that these microarrays are suitable for the high throughput characterization of the recognition capabilities of monoclonal antibodies, carbohydrate-binding modules, and other oligosaccharide-binding proteins of biological significance and also that they have potential for the characterization of carbohydrate-active enzymes. (megazyme.com)
  • ATP-binding cassette (ABC) transporters, a large class of transmembrane proteins, are widely found in organisms and play an important role in the transport of xenobiotics. (barcelonashop.shop)
  • ABC transporters are important in a wide range of physiological processes and can translocate a variety of substrates, including sugars, ions, lipids, and proteins (Rees et al. (barcelonashop.shop)
  • ABC transporters are a large family of proteins involved in the transport of a wide variety of different compounds, like sugars, ions, peptides, and more complex organic molecules. (umbc.edu)
  • ABC transporters are a subset of nucleotide hydrolases that contain a signature motif, Q-loop, and H-loop/switch region, in addition to, the Walker A motif/P-loop and Walker B motif commonly found in a number of ATP- and GTP-binding and hydrolyzing proteins. (umbc.edu)
  • BfrC and BfrE are ATP-binding proteins, and BfrD, BfrF and BfrG are homologous membrane-spanning polypeptides. (elsevier.com)
  • Synthetic therapeutics for antibiotic resistance in bacteria or multidrug resistance in tumour cells can block ATP-driven transport proteins that carries chemotherapeutics out of the cell. (uni-frankfurt.de)
  • There are currently only a few synthetic agents that bind to and block the widespread membrane transport proteins, ATP-binding cassette transporters (ABC). (uni-frankfurt.de)
  • Robert Tampé elucidated essential mechanisms of ATP-driven transport proteins and cellular machinery of adaptive immune response and quality control, which together with this new publication can provide approaches for applied drug research. (uni-frankfurt.de)
  • Here, we show that, unlike previously characterized DppA proteins, H. pylori DppA binds, with micromolar affinity, peptides of diverse amino acid sequences ranging between two and eight residues in length. (bioinfor.com)
  • micrornas (mirnas) are small regulatory rna molecules that inhibit the expression of specific target genes by binding to and cleaving their messenger rnas or otherwise inhibiting their translation into proteins. (liverpool.ac.uk)
  • Arsenic binding to proteins. (sciendo.com)
  • Ajees A.A., Rosen B.P.: As(III) S-adenosylmethionine methyltransferases and other arsenic binding proteins. (sciendo.com)
  • Examples of splicing events that were found to modulate transporter expression across individuals include alternate 5 prime untranslated regions (5' UTRs) in the genes ABCC5 and ABCA8. (escholarship.org)
  • Finally, transcription factor regulators of ABC transporters were identified by searching for transcription factor binding motif enrichment in sets of genes co-expressed with ABC transporters in several human tissues. (escholarship.org)
  • Increased binding of CEBPD to promoter regions was observed in GSCs, indicating the direct regulation of these GSC-related genes by CEBPD. (elsevier.com)
  • Multispecies genetic analysis has identified the MHC-E locus is the most conserved of all primate class I genes, especially in the peptide-binding region, underscoring the high degree of evolutionary conservation and the crucial biological function of the HLA-E-peptide complex ( 8 , 9 ). (aai.org)
  • Chemotherapy-induced resistance by ATP-binding cassette transporter genes. (semanticscholar.org)
  • 13 ABCG genes were identified in N. lugens, and expression levels of these ABCG transporter genes after treatment with thiamethoxam, abamectin, and cyantraniliprole has been examined. (tcdb.org)
  • Reversing multidrug resistance in breast cancer cells by silencing ABC transporter genes with nanoparticle-facilitated delivery of target siRNAs. (scielo.br)
  • Mutations in either of two genes comprising the STSL locus, ATP-binding cassette (ABC)-transporters ABCG5 (encoding sterolin-1) and ABCG8 (encoding sterolin-2), result in sitosterolemia, a rare autosomal recessive disorder of sterol trafficking characterized by increased plasma plant sterol levels. (biomedcentral.com)
  • The four murine peroxisomal ABC-transporter genes differ in constitutive, inducible and developmental expression. (medecinesciences.org)
  • The genetic factors include single-nucleotide polymorphisms (SNPs) in the genes of canalicular transporters, including ABCB4 and ABCB11 . (biomedcentral.com)
  • ATP-binding cassette transporter C4 (ABCC4) is associated with multidrug resistance and the regulation of cell signalling. (elsevier.com)
  • Multidrug transporters (MDRs) are a specific type of ABC transporter and are commonly overexpressed in cancer cells. (tocris.com)
  • P-glycoprotein (P-gp, ABCB1, MDR1) is a well-characterized human ABC transporter that was the first ABC transporter implicated in multidrug resistance. (tocris.com)
  • Due to the role of P-gp in extruding xenobiotics from a broad range of cells, this transporter also confers the multidrug resistance phenotype in many different cell types. (tocris.com)
  • Tocris offers the following scientific literature for Multidrug Transporters to showcase our products. (tocris.com)
  • When overexpressed, they function as ATP-dependent efflux pumps able to extrude lipophilic drugs (especially ANTINEOPLASTIC AGENTS ) from cells, causing multidrug resistance ( DRUG RESISTANCE, MULTIPLE ). (nih.gov)
  • ABC transporters are also central to multidrug resistance in many pathogenic bacteria and in tumor cells. (barcelonashop.shop)
  • They are ubiquitous transporters involved in the import of essential molecules in prokaryotes and the efflux of cytotoxic molecules in humans.These ubiquitous transporters have been implicated in multidrug resistance, a key clinical. (barcelonashop.shop)
  • However, the possible involvement of ABC transporters in clinical multidrug resistance is difficult to establish. (pensoft.net)
  • Indeed, the data obtained from in vitro experiments convincingly suggest that particular members of ABC transporter family can mediate multidrug resistance (MDR) in cancer cells. (pensoft.net)
  • Structure of MsbA from E. coli: a homolog of the multidrug resistance ATP binding cassette (ABC) transporters. (thewinnower.com)
  • Structure of MsbA from Vibrio cholera: a multidrug resistance ABC transporter homolog in a closed conformation. (thewinnower.com)
  • X-ray structure of the EmrE multidrug transporter in complex with a substrate. (thewinnower.com)
  • The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily. (mpg.de)
  • ABCG2 is expressed on the luminal membrane of retinal capillary endothelial cells, where ABCG2 acts as the efflux transporter for photosensitive toxins such as pheophorbide a and protoporphyrin IX. (elsevier.com)
  • Description: A sandwich ELISA kit for detection of ATP Binding Cassette Transporter C3 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (therabio.org)
  • Several expression quantitative trait loci (eQTLs) were identified for ABC transporters in the kidney, and one eQTL for ABCG2 (BCRP) was validated in an in vitro reporter-gene assay. (escholarship.org)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human ATP Binding Cassette Transporter G2 (ABCG2) in samples from tissue homogenates, cell lysates or other biological fluids. (glideruniversity.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse ATP Binding Cassette Transporter G2 (ABCG2) in Tissue homogenates and other biological fluids. (glideruniversity.org)
  • Double immunolabeling of ABCG2 and glucose transporter 1 suggested that ABCG2 was localized on the luminal membrane of mouse retinal capillary endothelial cells. (elsevier.com)
  • ABCG2, member of ATP-binding cassette (ABC) transporter family, is known as crucial regulator related to multi-drug resistance in human tumors and has recently been putatively studied as human carcinoma cell biomarker. (oncotarget.com)
  • ATP-binding cassette subfamily G, member 2 (ABCG2), is a member of the ATP-binding cassette (ABC) transporter family. (oncotarget.com)
  • Unfortunately, recent laboratory studies suggested that antiproliferative effect of DAS might be significantly reduced due to the overexpression of the ATP-binding cassette (ABC) transporters, ABCB1 and ABCG2. (pensoft.net)
  • Considering the fact that expression levels of ABCB1 and ABCG2 transporters are usually low in clinical samples, their contribution to the overall resistance to DAS is probably low but significant. (pensoft.net)
  • The ATP-binding cassette transporters (ABC transporters) are a transport system superfamily that is one of the largest and possibly one of the oldest gene families. (wikipedia.org)
  • ABC transporters belong to the ATP-Binding Cassette (ABC) superfamily, which uses the hydrolysis of ATP to energise diverse biological systems. (embl.de)
  • Members of the ATP‐binding cassette (ABC) transporter superfamily translocate a broad spectrum of chemically diverse substrates. (uni-frankfurt.de)
  • Dean M, Rzhetsky A, Allikmets R. The human ATP-binding cassette (ABC) transporter superfamily. (medecinesciences.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human ATP Binding Cassette Transporter C6 (ABCC6) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (myelisakit.com)
  • Description: A sandwich ELISA for quantitative measurement of Human ATP Binding Cassette Transporter G1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (unicoupi.com)
  • Description: A competitive ELISA for quantitative measurement of Human ATP Binding Cassette Transporter A1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (unicoupi.com)
  • The ATPase subunits utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to provide the energy needed for the translocation of substrates across membranes, either for uptake or for export of the substrate. (wikipedia.org)
  • ABC transporters utilize the energy of ATP binding and hydrolysis to transport various substrates across cellular membranes. (wikipedia.org)
  • The membrane-spanning region of the ABC transporter protects hydrophilic substrates from the lipids of the membrane bilayer thus providing a pathway across the cell membrane. (wikipedia.org)
  • A basic triad within the binding pocket was identified as the main binding motif for the substrates and was examined through site-directed mutagenesis and subsequent characterization of the variants through fluorescence spectroscopy and ligand-dependent melting point analysis. (uni-marburg.de)
  • Crystallographic and CD-spectroscopic experiments showed that the binding pocket accepts solely lambda-stereoconfigured substrates, therefore the delta-stereoconfiguration of the iron-complex of enterobactin is inverted through binding by FeuA. (uni-marburg.de)
  • ABC transporters are involved in the export or import of a wide variety of substrates ranging from small ions to macromolecules. (embl.de)
  • Mammalian peroxisomal ABC transporters: from endogenous substrates to pathology and clinical significance. (medecinesciences.org)
  • ABC transporters are ubiquitous in all kingdoms of life and facilitate the transport of a large variety of substrates across membranes using ATP hydrolysis as energy source [6, 7]. (barcelonashop.shop)
  • ABC Transporters.ATP-binding cassette (ABC) transporters constitute the largest family of primary active transporters, responsible for many physiological processes and human maladies.ABC transporters are fascinating molecular machines that use the energy of ATP hydrolysis to drive the movement of substrates across the lipid membranes. (barcelonashop.shop)
  • Unlike the highly polymorphic MHC class Ia molecules that bind a wide array of peptides, HLA-E binds a limited set of highly conserved hydrophobic nonamer peptides originating from class Ia leader sequences ( 1 , 2 , 3 , 4 ) for presentation to the CD94/NKG2 family of receptors found on the surface of NK and T cells ( 5 , 6 , 7 ). (aai.org)
  • ABC transporters minimally contain two conserved regions: a highly conserved ATP binding cassette (ABC) and a less conserved transmembrane domain (TMD). (embl.de)
  • While their eponymous ATP‐binding cassette in the nucleotide‐binding domains (NBDs) is highly conserved, their transmembrane domains (TMDs) forming the translocation pathway exhibit distinct folds and topologies, suggesting that during evolution the ancient motor domains were combined with different transmembrane mechanical systems to orchestrate a variety of cellular processes. (uni-frankfurt.de)
  • These include targeting the adenosine triphosphate (ATP)-binding cassette (ABC) transporters, inducing cell apoptosis, inhibiting DNA repair, regulating metabolic reprogramming, or using combination therapy. (naturalnews.com)
  • Adenosine triphosphate-binding cassette (ABC) transporters play a critical role in keeping neurotoxic substances from entering the brain and in transporting toxic metabolites out of the brain [ 1 , 2 ]. (biomedcentral.com)
  • 2021 ) ATP-binding cassette transporters at the zebrafish blood-brain barrier and the potential utility of the zebrafish as an model. (academictree.org)
  • Apr 28, 2021 · In humans, ABC transporters make up one of the largest gene families, and more than a dozen genetic diseases have been traced to ABC transporter defects. (barcelonashop.shop)
  • The transporter P-glycoprotein (P-gp or MDR1), a product of the MDR1 gene, is responsible for resistance to doxorubicin and taxanes in cultured cells ( 7 7. (scielo.br)
  • Tsybovsky Y, Molday RS, Palczewski K. The ATP-binding cassette transporter ABCA4: structural and functional properties and role in retinal disease. (medlineplus.gov)
  • ATP-binding cassette transporters cardiovascular disease cholesterol transport lipid metabolism sitosterolemia structural biology. (silverchair.com)
  • ATP-binding cassette transporters, ABCA1 and ABCG1, are cell surface transporters that mediate the reverse cholesterol transport (RCT), a process that delivers cholesterol from the peripheral tissue back to the liver for disposal [ 2 ]. (alliedacademies.org)
  • ATP-binding cassette (ABC) transporters constitute the largest family of primary active transporters, responsible for many physiological processes and human maladies.However, various drug transporters, including solute carrier (SLC) transporters and ATP-binding cassette (ABC) transporters located in the single layer of polarized syncytiotrophoblasts in placenta. (barcelonashop.shop)
  • However, whether these drug transporters might compromise therapeutic effect of DAS in clinic is unclear. (pensoft.net)
  • ABC transporters are also involved in multiple drug resistance, and this is how some of them were first identified. (wikipedia.org)
  • Therefore, the understanding of certain related pathways in carbohydrate metabolism, amino acid metabolism, and ABC transporters could provide a provoking insight for a more specific genetic modification platform to strengthen plant resistance systems. (researchsquare.com)
  • Resistance-nodulation-cell division (RND) transporter AcrD confers resistance to egg white in Salmonella Enteritidis. (usda.gov)
  • Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration. (mpg.de)
  • In cancer, decreased MCJ expression is correlated with ATP binding cassette (ABC) transporter mediated chemotherapy resistance and poor patient responses. (uvm.edu)
  • The identification of cancer stem cells, or tumor initiating cells, has focused renewed attention on the role of ABC transporters in drug resistance, and as cancer therapy targets. (barcelonashop.shop)
  • Data implicated the role of altered membrane fluidity, decreased drug accumulation, increased expression of ABC transporters and higher tolerance capacity of parasite towards host defence mechanism in conferring PMM resistance in Leishmania. (itg.be)
  • What happens when they encounter the ATP-driven transport molecule that is responsible for resistance by carrying the chemotherapeutic agents out of the cell? (uni-frankfurt.de)
  • Mlejnek P, Dolezel P, Ruzickova E (2017) Drug resistance of cancer cells is crucially affected by expression levels of ABC-transporters. (pensoft.net)
  • The lower expression levels of ABC transporters mediate lower resistance. (pensoft.net)
  • We observed that the expression level of the studied ABC-transporter is an important factor that affects the cell resistance. (pensoft.net)
  • Subtle changes in endogenous expression level of these transporters can have significant clinical implications. (escholarship.org)
  • MRP1 is a single polypeptide comprising three transmembrane domains (TMD0, TMD1, and TMD2) and two cytosolic nucleotide-binding domains (NBD1 and NBD2). (elifesciences.org)
  • ATP-binding cassette transporter A1 (ABCA1) is a cholesterol transporter that transfers surplus mobile cholesterol onto lipid-poor apolipoproteins. (kn-62.com)
  • Molday RS, Zhong M, Quazi F. The role of the photoreceptor ABC transporter ABCA4 in lipid transport and Stargardt macular degeneration. (medlineplus.gov)
  • This study investigates the effect of genistein (Gen) on the lipid profiles and expression of low-density lipoprotein receptor (LDLR), liver X receptor α (LXRα) and ATP-binding cassette transporter G1 (ABCG1) in the plasma macrophages of postmenopausal women with hyperlipidemia in China. (biomedcentral.com)
  • Mutations in lipid transporter ABCA12 in harlequin ichthyosis and functional recovery by corrective gene transfer.The similarities and differences between TAP and other transporters support the notion that ABC transporters in general have adapted around a universal transport mechanism. (barcelonashop.shop)
  • lipid metabolism lipid-binding domains membranes phosphatidylinositol polyunsaturated fatty acids signalling. (silverchair.com)
  • The ATP Binding Cassette Transporter ( ABCA1 ) is a key gene involved in lipid metabolism. (biomedcentral.com)
  • We are therefore very interested in understanding the mechanisms by which this cassette couples the process of ATP hydrolysis to transport. (the-scientist.com)
  • ABCG46 of the legume Medicago truncatula is an ABC-type plasma membrane transporter that selectively translocates endogenous phenylpropanoids from the biosynthetic pathway of the phytoalexin, medicarpin, namely 4-coumarate and liquiritigenin. (researchgate.net)
  • Therefore, modulation of endogenous ABCA transporters may potentially Kaempferol inhibition have a substantial impact on mind function and susceptibility to neurodegenerative illnesses. (stopvivisection.info)
  • The ATP-binding cassette transporter G1 (ABCG1) was recently identified as a regulator of macrophage cholesterol and phospholipid transport. (lih.lu)
  • The expression of ATP-binding cassette-transporter G1 (ABCG1) was also reduced in the ORP11 knock-down cells. (helsinki.fi)
  • ATP-binding cassette transporter G1 (ABCG1) is a key molecule for cholesterol efflux from macrophages and HDL biogenesis. (biomedcentral.com)
  • 2007. Biotin uptake in prokaryotes by solute transporters with an optional ATP-binding cassette-containing module. (hu-berlin.de)
  • MTs express plenty of organic solute transporters, indicating the tubules have the capability to actively excrete the broadest range of organic solutes (e.g., sugar, amino acid and multivitamin) [ 4 ]. (biomedcentral.com)
  • Homozygous mutations including deletions and polymorphisms of the ATP-binding cassette transporter 1 (ABCA1) cause Tangier disease, a rare autosomal recessive disorder with congenital HDL deficiency and increased risk of atherosclerosis. (biomedcentral.com)
  • 1/ Structure, function, molecular mechanism and physiological role of ABC transporters. (doktori.hu)
  • The role of abc transporters in drug absorption, distribution, metabolism, excretion and toxicity (adme-tox) , DRUG DISCOVERY TODAY 13: (9-10) pp. 379-393. (doktori.hu)
  • 2010. Subunit composition of an energy-coupling-factor-type biotin transporter analysed in living bacteria. (hu-berlin.de)
  • ATP-binding cassette transporter-1 (ABCA1) gene is mutated in patients with familial high-density lipoprotein deficiency (FHD). (uni.lu)
  • ABCA4 is an ATP-binding cassette (ABC) transporter that flips N-retinylidene-phosphatidylethanolamine (N-Ret-PE) from the lumen to the cytoplasmic leaflet of photoreceptor membranes. (nature.com)
  • To define the mechanisms underlying substrate binding and STGD1, we determine the cryo-EM structure of ABCA4 in its substrate-free and bound states. (nature.com)
  • Bacterial ABC transporters are essential in cell viability, virulence, and pathogenicity. (wikipedia.org)
  • 2014. Transport of nickel and cobalt ions into bacterial cells by S components of ECF transporters. (hu-berlin.de)
  • In certain bacterial transporters, these regions are found on different polypeptides. (embl.de)
  • NrtD and SsuB are the ATP-binding subunits of the bacterial ABC-type nitrate and sulfonate transport systems, respectively. (umbc.edu)
  • HLA-E is an MHC class Ib molecule that binds nonamer peptides derived from the leader sequence of MHC class 1a molecules and is the major ligand for CD94/NKG2 receptors found on NK and T cells. (aai.org)
  • In this outward-facing (OF) conformation, two ATP molecules are occluded at the NBD dimer interface: one in the catalytically inactive, degenerate site and the other in the active consensus site, poised for hydrolysis ( Figure 1A ). (elifesciences.org)
  • Loading onto major histocompatibility complex class I molecules and proofreading of these bound epitopes are orchestrated within the macromolecular peptide-loading complex, which assembles on TAP. (uni-frankfurt.de)
  • Homo- and heterodimerization of peroxisomal ATP-binding cassette half-transporters. (medecinesciences.org)
  • La maturiĝintaj eritrocitoj de la homo (kaj la mamuloj) ne havas ĉelkernon (kontraste al birdoj kaj reptilioj, kiuj havas ĉelkernon en la eritrocito). (dbpedia.org)
  • Introduction ATP-binding cassette (ABC) transporters are categorized into seven subfamilies regarding to their framework and sequence homology. (stopvivisection.info)
  • Finally, we show that the BfrA DNA-binding domain recognizes a conserved DNA motif with a consensus sequence of TTTCTTTAGAAATATTTTAGAATT. (elsevier.com)
  • Kinetic coupling of the respiratory chain with ATP synthase, but not proton gradients, drives ATP production in cristae membranes. (mpg.de)
  • The compounds have potential as therapeutic agents to treat S. aureus infections, and purification of the transmembrane transporter will enable further development. (elsevier.com)
  • An ATP-binding cassette (ABC) transporter located in the inner mitochondrial membrane is involved in iron-sulfur cluster and molybdenum cofactor assembly in the cytosol, but the transported substrate is unknown. (uea.ac.uk)
  • The projects aim to examine different aspects of the genetics of the species such as ATP-binding cassette (ABC) transporters, landscape genomics in China, phylogeographical analysis of DBM and its parasitoid Cotesia vestalis using mitochondrial and nuclear markers, etc. (brocku.ca)
  • MCJ deficiency enhances the immune effector functions and memory responses of CD8+ T cells in a mitochondrial ATP dependent manner. (uvm.edu)
  • Increased mitochondrial ATP production due to MCJ deficiency is sufficient to fuel ABC transporter activity, thereby directly promoting chemoresistance. (uvm.edu)
  • For instance, a potential lethal increase in osmotic strength is counterbalanced by activation of osmosensing ABC transporters that mediate uptake of solutes. (wikipedia.org)
  • 2019. ECF-type ABC transporters for uptake of vitamins and transition metal ions into prokaryotic cells. (hu-berlin.de)
  • Despite numerous studies that have focused on carbohydrate uptake systems in closely related streptococcal species such as S. mutans, S. pneumonia and S. pyogenes, the mechanism of the response of these ABC transporters to acidic conditions in S. sanguinis is still unknown. (vcu.edu)
  • Uptake of certain peptides by H. pylori requires an ATP binding cassette (ABC) transporter annotated dipeptide permease (Dpp). (bioinfor.com)
  • In this article, we review recent advances in the understanding of arsenic toxicity and its transport routes in prokaryotes and eukaryotes, including a dual role of aquaglyceroporins in the uptake and efflux, active transport out of the cell via secondary ion pumps and sequestration of metalloid-thiol conjugates into vacuoles by primary ABC transporters. (sciendo.com)
  • We purified, for the first time, a WTA transporter and have reconstituted ATPase activity in proteoliposomes. (elsevier.com)
  • These are the first ABC transporter inhibitors shown to block ATPase activity by binding to the transmembrane domain. (elsevier.com)
  • ATPase components of ABC transporters with duplicated ATPase domains [General function prediction. (systemsbiology.net)
  • In addition, the ring-shaped active substances are restricted in their spatial structure, so they bind to the target molecule without major rearrangements. (uni-frankfurt.de)
  • It is the event of the binding of the ATP molecule that pushes substances out of the cell. (uni-frankfurt.de)
  • ATP-binding cassette (ABC) transporters are molecular pumps ubiquitous across all kingdoms of life. (elifesciences.org)
  • In all kingdoms of life, ATP binding cassette (ABC) transporters are essential to many cellular functions. (vcu.edu)
  • The peroxisomal fatty acid transporter ABCD1/PMP-4 is required in the C. elegans hypodermis for axonal maintenance: A worm model for adrenoleukodystrophy. (cam.ac.uk)
  • Adrenoleukodystrophy is a neurometabolic disorder caused by a defective peroxisomal ABCD1 transporter of very long-chain fatty acids (VLCFAs). (cam.ac.uk)
  • Putative X-linked adrenoleukodystrophy gene shares unexpected homology with ABC transporters. (medecinesciences.org)
  • Functional overlap between ABCD1 (ALD) and ABCD2 (ALDR) transporters: a therapeutic target for X-adrenoleukodystrophy. (medecinesciences.org)
  • good guys online An ABC transporter (ATP-binding cassette transporter) is a 'member of a large family of membrane transporters with sequences that make up ATP-binding cassettes'.Feb 18, 2009 · ABC Transporters in Cancer Stem Cells. (barcelonashop.shop)
  • Cascorbi I. Role of pharmacogenetics of ATP-binding cassette transporters in the pharmacokinetics of drugs. (scielo.br)
  • Previously, we identified two positively cooperative drug-binding sites of P-glycoprotein involved in transport [Shapiro, A. B. & Ling, V. (1997) Eur. (nih.gov)
  • Substrate binding to one site stimulates transport by the other. (nih.gov)
  • These results indicate the existence of a third drug-binding site on P-glycoprotein with a positive allosteric effect on drug transport by the H and R sites. (nih.gov)
  • The rate-limiting step of the transport cycle is the dissociation of the nucleotide-binding-domain dimer, while ATP hydrolysis per se does not reset MRP1 to the resting state. (elifesciences.org)
  • 2017. Complex stability during the transport cycle of a subclass I ECF transporter. (hu-berlin.de)
  • Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. (researchgate.net)
  • However, long-term exposure of the juvenile brain to low-dose of ET-1 did not change BBB P-gp transport activity but tended to decrease BCRP transport activity in the juvenile brain, while a significant increase of the activity of both transporters was evidenced at the BBB in the adult brain. (biomedcentral.com)
  • This heterodimeric ABC-binding cassette (ABC) transport complex is therefore a major component in the adaptive immune response against virally or malignantly transformed cells. (uni-frankfurt.de)
  • This can happen at the beginning or at the end of a transport process, when the transporter is in a resting state. (uni-frankfurt.de)
  • These insights have already led to a "paradigm shift" in science, as Tampé explains: "Until now, we have assumed that ATP hydrolysis (note: an energy-releasing splitting process) provides the energy for transport through the membrane. (uni-frankfurt.de)
  • The role of noncoding microRNAs in the control of drug metabolism and disposition through targeting of cytochrome P450 (P450) enzymes and ATP-binding cassette membrane transporters was discussed. (escholarship.org)
  • The transporter associated with antigen processing (TAP) selectively translocates antigenic peptides into the endoplasmic reticulum. (uni-frankfurt.de)
  • Auxin reconfigures the actin cytoskeleton, which controls polar localization of auxin transporters such as PIN2 and thus determines cell-type-specific responses. (researchgate.net)
  • 2012. Essential amino acid residues of BioY reveal that dimers are the functional S unit of the Rhodobacter capsulatus biotin transporter. (hu-berlin.de)
  • MDR is a complicated process that involves many mechanisms, one of which is the overexpression of energy-dependent transporters of anti-cancer drugs into and out of cells ( 5 5. (scielo.br)
  • This report is the first to identify a non-MHC leader inhibitory peptide bound to HLA-E and provides insight into the immunoregulatory role of HLA-E during cell stress. (aai.org)
  • This high degree of peptide specificity is optimized for binding class Ia leader sequences, and in the event of insufficient leader peptide, as may occur following viral infection or during tumorogenesis, surface HLA-E is diminished and renders a cell susceptible to NK cell-mediated lysis due to insufficient ligand density to engage the CD94/NKG2A receptor. (aai.org)
  • However, the observation also revealed that these two were not specific inhibitors of ABC transporters. (naturalnews.com)
  • Understanding the binding mode of antigenic peptides in the TAP complex will crucially impact rational design of inhibitors, drug development, or vaccination strategies. (uni-frankfurt.de)
  • Tyrosine kinase inhibitors (TKIs), low molecular weight inhibitors that compete with the ATP binding site of the catalytic domain of oncogenic tyrosine kinases, exhibit effective antitumor activity both in vitro and in vivo . (pensoft.net)
  • 2014. A versatile Escherichia coli strain for identification of biotin transporters and for biotin quantification. (hu-berlin.de)