A superfamily of large integral ATP-binding cassette membrane proteins whose expression pattern is consistent with a role in lipid (cholesterol) efflux. It is implicated in TANGIER DISEASE characterized by accumulation of cholesteryl ester in various tissues.
A family of MEMBRANE TRANSPORT PROTEINS that require ATP hydrolysis for the transport of substrates across membranes. The protein family derives its name from the ATP-binding domain found on the protein.
The most abundant protein component of HIGH DENSITY LIPOPROTEINS or HDL. This protein serves as an acceptor for CHOLESTEROL released from cells thus promoting efflux of cholesterol to HDL then to the LIVER for excretion from the body (reverse cholesterol transport). It also acts as a cofactor for LECITHIN CHOLESTEROL ACYLTRANSFERASE that forms CHOLESTEROL ESTERS on the HDL particles. Mutations of this gene APOA1 cause HDL deficiency, such as in FAMILIAL ALPHA LIPOPROTEIN DEFICIENCY DISEASE and in some patients with TANGIER DISEASE.
A sequence-related subfamily of ATP-BINDING CASSETTE TRANSPORTERS that actively transport organic substrates. Although considered organic anion transporters, a subset of proteins in this family have also been shown to convey drug resistance to neutral organic drugs. Their cellular function may have clinical significance for CHEMOTHERAPY in that they transport a variety of ANTINEOPLASTIC AGENTS. Overexpression of proteins in this class by NEOPLASMS is considered a possible mechanism in the development of multidrug resistance (DRUG RESISTANCE, MULTIPLE). Although similar in function to P-GLYCOPROTEINS, the proteins in this class share little sequence homology to the p-glycoprotein family of proteins.
An autosomal recessively inherited disorder caused by mutation of ATP-BINDING CASSETTE TRANSPORTERS involved in cellular cholesterol removal (reverse-cholesterol transport). It is characterized by near absence of ALPHA-LIPOPROTEINS (high-density lipoproteins) in blood. The massive tissue deposition of cholesterol esters results in HEPATOMEGALY; SPLENOMEGALY; RETINITIS PIGMENTOSA; large orange tonsils; and often sensory POLYNEUROPATHY. The disorder was first found among inhabitants of Tangier Island in the Chesapeake Bay, MD.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A broad category of receptor-like proteins that may play a role in transcriptional-regulation in the CELL NUCLEUS. Many of these proteins are similar in structure to known NUCLEAR RECEPTORS but appear to lack a functional ligand-binding domain, while in other cases the specific ligands have yet to be identified.
The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
A 170-kDa transmembrane glycoprotein from the superfamily of ATP-BINDING CASSETTE TRANSPORTERS. It serves as an ATP-dependent efflux pump for a variety of chemicals, including many ANTINEOPLASTIC AGENTS. Overexpression of this glycoprotein is associated with multidrug resistance (see DRUG RESISTANCE, MULTIPLE).
A class of lipoproteins of small size (4-13 nm) and dense (greater than 1.063 g/ml) particles. HDL lipoproteins, synthesized in the liver without a lipid core, accumulate cholesterol esters from peripheral tissues and transport them to the liver for re-utilization or elimination from the body (the reverse cholesterol transport). Their major protein component is APOLIPOPROTEIN A-I. HDL also shuttle APOLIPOPROTEINS C and APOLIPOPROTEINS E to and from triglyceride-rich lipoproteins during their catabolism. HDL plasma level has been inversely correlated with the risk of cardiovascular diseases.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Lipid-protein complexes involved in the transportation and metabolism of lipids in the body. They are spherical particles consisting of a hydrophobic core of TRIGLYCERIDES and CHOLESTEROL ESTERS surrounded by a layer of hydrophilic free CHOLESTEROL; PHOSPHOLIPIDS; and APOLIPOPROTEINS. Lipoproteins are classified by their varying buoyant density and sizes.
The process of cleaving a chemical compound by the addition of a molecule of water.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
Transport proteins that carry specific substances in the blood or across cell membranes.
Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.
Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Established cell cultures that have the potential to propagate indefinitely.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
Proteins found in any species of fungus.
A subfamily of transmembrane proteins from the superfamily of ATP-BINDING CASSETTE TRANSPORTERS that are closely related in sequence to P-GLYCOPROTEIN. When overexpressed, they function as ATP-dependent efflux pumps able to extrude lipophilic drugs, especially ANTINEOPLASTIC AGENTS, from cells causing multidrug resistance (DRUG RESISTANCE, MULTIPLE). Although P-Glycoproteins share functional similarities to MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS they are two distinct subclasses of ATP-BINDING CASSETTE TRANSPORTERS, and have little sequence homology.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Simultaneous resistance to several structurally and functionally distinct drugs.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Elements of limited time intervals, contributing to particular results or situations.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Proteins found in any species of bacterium.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
The ability of fungi to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance phenotype may be attributed to multiple gene mutations.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A family of sterols commonly found in plants and plant oils. Alpha-, beta-, and gamma-isomers have been characterized.
The rate dynamics in chemical or physical systems.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
A family of scavenger receptors that are predominately localized to CAVEOLAE of the PLASMA MEMBRANE and bind HIGH DENSITY LIPOPROTEINS.
Proteins obtained from ESCHERICHIA COLI.
An oligopeptide produced by various bacteria which acts as a protease inhibitor.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Proteins involved in the transport of organic anions. They play an important role in the elimination of a variety of endogenous substances, xenobiotics and their metabolites from the body.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
An anthracenedione-derived antineoplastic agent.
An antidiabetic sulfonylurea derivative with actions similar to those of chlorpropamide.
A class of organic compounds known as STEROLS or STEROIDS derived from plants.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Chemical substances that are foreign to the biological system. They include naturally occurring compounds, drugs, environmental agents, carcinogens, insecticides, etc.
The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Resistance or diminished response of a neoplasm to an antineoplastic agent in humans, animals, or cell or tissue cultures.
The process by which two molecules of the same chemical composition form a condensation product or polymer.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
An X-linked recessive disorder characterized by the accumulation of saturated very long chain fatty acids in the LYSOSOMES of ADRENAL CORTEX and the white matter of CENTRAL NERVOUS SYSTEM. This disease occurs almost exclusively in the males. Clinical features include the childhood onset of ATAXIA; NEUROBEHAVIORAL MANIFESTATIONS; HYPERPIGMENTATION; ADRENAL INSUFFICIENCY; SEIZURES; MUSCLE SPASTICITY; and DEMENTIA. The slowly progressive adult form is called adrenomyeloneuropathy. The defective gene ABCD1 is located at Xq28, and encodes the adrenoleukodystrophy protein (ATP-BINDING CASSETTE TRANSPORTERS).
Proteins prepared by recombinant DNA technology.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Steroids with a hydroxyl group at C-3 and most of the skeleton of cholestane. Additional carbon atoms may be present in the side chain. (IUPAC Steroid Nomenclature, 1987)
A dextrodisaccharide from malt and starch. It is used as a sweetening agent and fermentable intermediate in brewing. (Grant & Hackh's Chemical Dictionary, 5th ed)
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The product of conjugation of cholic acid with taurine. Its sodium salt is the chief ingredient of the bile of carnivorous animals. It acts as a detergent to solubilize fats for absorption and is itself absorbed. It is used as a cholagogue and cholerectic.
Cholesterol which is contained in or bound to high-density lipoproteins (HDL), including CHOLESTEROL ESTERS and free cholesterol.
The relationship between the dose of an administered drug and the response of the organism to the drug.
A thickening and loss of elasticity of the walls of ARTERIES that occurs with formation of ATHEROSCLEROTIC PLAQUES within the ARTERIAL INTIMA.
A ubiquitously expressed glucose transporter that is important for constitutive, basal GLUCOSE transport. It is predominately expressed in ENDOTHELIAL CELLS and ERYTHROCYTES at the BLOOD-BRAIN BARRIER and is responsible for GLUCOSE entry into the BRAIN.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Diminished or failed response of an organism, disease or tissue to the intended effectiveness of a chemical or drug. It should be differentiated from DRUG TOLERANCE which is the progressive diminution of the susceptibility of a human or animal to the effects of a drug, as a result of continued administration.
Membrane transporters that co-transport two or more dissimilar molecules in the same direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.
Sodium chloride-dependent neurotransmitter symporters located primarily on the PLASMA MEMBRANE of serotonergic neurons. They are different than SEROTONIN RECEPTORS, which signal cellular responses to SEROTONIN. They remove SEROTONIN from the EXTRACELLULAR SPACE by high affinity reuptake into PRESYNAPTIC TERMINALS. Regulates signal amplitude and duration at serotonergic synapses and is the site of action of the SEROTONIN UPTAKE INHIBITORS.
A family of proteins involved in the transport of monocarboxylic acids such as LACTIC ACID and PYRUVIC ACID across cellular membranes.
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
Sodium chloride-dependent neurotransmitter symporters located primarily on the PLASMA MEMBRANE of dopaminergic neurons. They remove DOPAMINE from the EXTRACELLULAR SPACE by high affinity reuptake into PRESYNAPTIC TERMINALS and are the target of DOPAMINE UPTAKE INHIBITORS.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A cell line derived from cultured tumor cells.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Bactericidal cationic quaternary ammonium surfactant used as a topical anti-infective agent. It is an ingredient in medicaments, deodorants, mouthwashes, etc., and is used to disinfect apparatus, etc., in the food processing and pharmaceutical industries, in surgery, and also as a preservative. The compound is toxic orally as a result of neuromuscular blockade.
Lipid-laden macrophages originating from monocytes or from smooth muscle cells.
Membrane proteins whose primary function is to facilitate the transport of negatively charged molecules (anions) across a biological membrane.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
A glutamate plasma membrane transporter protein found in ASTROCYTES and in the LIVER.
Oxyvanadium ions in various states of oxidation. They act primarily as ion transport inhibitors due to their inhibition of Na(+)-, K(+)-, and Ca(+)-ATPase transport systems. They also have insulin-like action, positive inotropic action on cardiac ventricular muscle, and other metabolic effects.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A neuronal and epithelial type glutamate plasma membrane transporter protein.
Membrane proteins whose primary function is to facilitate the transport of positively charged molecules (cations) across a biological membrane.
5'-Adenylic acid, monoanhydride with imidodiphosphoric acid. An analog of ATP, in which the oxygen atom bridging the beta to the gamma phosphate is replaced by a nitrogen atom. It is a potent competitive inhibitor of soluble and membrane-bound mitochondrial ATPase and also inhibits ATP-dependent reactions of oxidative phosphorylation.
A family of POTASSIUM and SODIUM-dependent acidic amino acid transporters that demonstrate a high affinity for GLUTAMIC ACID and ASPARTIC ACID. Several variants of this system are found in neuronal tissue.
Organic or inorganic compounds that contain the -N3 group.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A chloride channel that regulates secretion in many exocrine tissues. Abnormalities in the CFTR gene have been shown to cause cystic fibrosis. (Hum Genet 1994;93(4):364-8)
An organic cation transporter found in kidney. It is localized to the basal lateral membrane and is likely to be involved in the renal secretion of organic cations.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A glial type glutamate plasma membrane transporter protein found predominately in ASTROCYTES. It is also expressed in HEART and SKELETAL MUSCLE and in the PLACENTA.
Sodium chloride-dependent neurotransmitter symporters located primarily on the PLASMA MEMBRANE of noradrenergic neurons. They remove NOREPINEPHRINE from the EXTRACELLULAR SPACE by high affinity reuptake into PRESYNAPTIC TERMINALS. It regulates signal amplitude and duration at noradrenergic synapses and is the target of ADRENERGIC UPTAKE INHIBITORS.
Salts and esters of CHOLIC ACID.

Alternative sulfonylurea receptor expression defines metabolic sensitivity of K-ATP channels in dopaminergic midbrain neurons. (1/7249)

ATP-sensitive potassium (K-ATP) channels couple the metabolic state to cellular excitability in various tissues. Several isoforms of the K-ATP channel subunits, the sulfonylurea receptor (SUR) and inwardly rectifying K channel (Kir6.X), have been cloned, but the molecular composition and functional diversity of native neuronal K-ATP channels remain unresolved. We combined functional analysis of K-ATP channels with expression profiling of K-ATP subunits at the level of single substantia nigra (SN) neurons in mouse brain slices using an RT-multiplex PCR protocol. In contrast to GABAergic neurons, single dopaminergic SN neurons displayed alternative co-expression of either SUR1, SUR2B or both SUR isoforms with Kir6.2. Dopaminergic SN neurons expressed alternative K-ATP channel species distinguished by significant differences in sulfonylurea affinity and metabolic sensitivity. In single dopaminergic SN neurons, co-expression of SUR1 + Kir6.2, but not of SUR2B + Kir6.2, correlated with functional K-ATP channels highly sensitive to metabolic inhibition. In contrast to wild-type, surviving dopaminergic SN neurons of homozygous weaver mouse exclusively expressed SUR1 + Kir6.2 during the active period of dopaminergic neurodegeneration. Therefore, alternative expression of K-ATP channel subunits defines the differential response to metabolic stress and constitutes a novel candidate mechanism for the differential vulnerability of dopaminergic neurons in response to respiratory chain dysfunction in Parkinson's disease.  (+info)

Inward rectification in KATP channels: a pH switch in the pore. (2/7249)

Inward-rectifier potassium channels (Kir channels) stabilize the resting membrane potential and set a threshold for excitation in many types of cell. This function arises from voltage-dependent rectification of these channels due to blockage by intracellular polyamines. In all Kir channels studied to date, the voltage-dependence of rectification is either strong or weak. Here we show that in cardiac as well as in cloned KATP channels (Kir6.2 + sulfonylurea receptor) polyamine-mediated rectification is not fixed but changes with intracellular pH in the physiological range: inward-rectification is prominent at basic pH, while at acidic pH rectification is very weak. The pH-dependence of polyamine block is specific for KATP as shown in experiments with other Kir channels. Systematic mutagenesis revealed a titratable C-terminal histidine residue (H216) in Kir6.2 to be the structural determinant, and electrostatic interaction between this residue and polyamines was shown to be the molecular mechanism underlying pH-dependent rectification. This pH-dependent block of KATP channels may represent a novel and direct link between excitation and intracellular pH.  (+info)

Overexpression of the multidrug resistance-associated protein (MRP1) in human heavy metal-selected tumor cells. (3/7249)

Cellular and molecular mechanisms involved in the resistance to cytotoxic heavy metals remain largely to be characterized in mammalian cells. To this end, we have analyzed a metal-resistant variant of the human lung cancer GLC4 cell line that we have selected by a step-wise procedure in potassium antimony tartrate. Antimony-selected cells, termed GLC4/Sb30 cells, poorly accumulated antimony through an enhanced cellular efflux of metal, thus suggesting up-regulation of a membrane export system in these cells. Indeed, GLC4/Sb30 cells were found to display a functional overexpression of the multidrug resistance-associated protein MRP1, a drug export pump, as demonstrated by Western blotting, reverse transcriptase-polymerase chain reaction and calcein accumulation assays. Moreover, MK571, a potent inhibitor of MRP1 activity, was found to markedly down-modulate resistance of GLC4/Sb30 cells to antimony and to decrease cellular export of the metal. Taken together, our data support the conclusion that overexpression of functional MRP1 likely represents one major mechanism by which human cells can escape the cytotoxic effects of heavy metals.  (+info)

Neural modulation of cephalexin intestinal absorption through the di- and tripeptide brush border transporter of rat jejunum in vivo. (4/7249)

Intestinal absorption of beta-lactamine antibiotics (e.g., cefixime and cephalexin) has been shown to proceed through the dipeptide carrier system. In a previous study, nifedipine (NFP), an L-type calcium channel blocker, enhanced the absorption of cefixime in vivo but not in vitro, and it was suggested that neural mechanisms might be involved in the effect of NFP. The aim of the present study was to assess the involvement of the nervous system on the intestinal absorption of cephalexin (CFX). To investigate this, we used a single-pass jejunal perfusion technique in rats. NFP and diltiazem enhanced approximately 2-fold the plasma levels of CFX in treated rats versus untreated controls. NFP also increased approximately 2-fold the CFX level in portal plasma and increased urinary excretion of CFX, thus indicating that CFX did effectively increase CFX intestinal absorption. Perfusing high concentrations of dipeptides in the jejunal lumen competitively reduced CFX absorption and inhibited the enhancement of CFX absorption produced by NFP. Hexamethonium and lidocaine inhibited the effect of NFP, whereas atropine, capsaicin, clonidine, and isoproterenol enhanced CFX absorption by the same order of magnitude as NFP. Thus, complex neural networks can modulate the function of the intestinal di- and tripeptide transporter. Sympathetic noradrenergic fibers, intestinal sensory neurons, and nicotinic synapses are involved in the increase of CFX absorption produced by NFP.  (+info)

Expression of atrC - encoding a novel member of the ATP binding cassette transporter family in Aspergillus nidulans - is sensitive to cycloheximide. (5/7249)

A new member of the ABC superfamily of transmembrane proteins in Aspergillus nidulans has been cloned and characterized. The topology of conserved motifs subgroups AtrC in the P-glycoprotein cluster of ABC permeases, the members of this subfamily, are known to participate in multidrug resistance (MDR) in diverse organisms. Alignment results display significant amino acid similarity to AfuMDR1 and AflMDR1 from Aspergillus fumigatus and flavus, respectively. Northern analysis reveals that atrC mRNA levels are 10-fold increased in response to cycloheximide. Evidence for the existence of eight additional hitherto unpublished ABC transporter proteins in A. nidulans is provided.  (+info)

MalK forms a dimer independent of its assembly into the MalFGK2 ATP-binding cassette transporter of Escherichia coli. (6/7249)

The maltose transport complex (MTC) is a member of the ATP-binding cassette superfamily of membrane transport proteins and is a model for understanding the folding and assembly of hetero-oligomeric membrane protein complexes. The MTC is made up of two integral membrane proteins, MalF and MalG, and a peripheral membrane protein, MalK. These proteins associate with a stoichiometry of 1:1:2 to form the complex MalFGK2. In our studies of the oligomerization of this complex, we have shown that the ATP-binding component, MalK, forms a dimer in the absence of MalF and MalG. Epitope-tagged MalK coimmunoprecipitated with wild-type MalK, indicating that the MalK protein forms an oligomer. The relative amounts of tagged and wild-type MalK that were present in the whole cell extracts and in the immunoprecipitated complexes show that the MalK oligomer is a dimer. These hetero-oligomers can also be formed in vitro by mixing two extracts, each containing either tagged or wild-type MalK. The dimerization of MalK was also demonstrated in vivo using the bacteriophage lambda repressor fusion assay. The formation of a MalK dimer in the absence of MalF and MalG may represent an initial step in the assembly pathway of the MTC.  (+info)

Glucose-receptive neurones in the rat ventromedial hypothalamus express KATP channels composed of Kir6.1 and SUR1 subunits. (7/7249)

1. Patch-clamp recordings were made from rat ventromedial hypothalamic neurones in slices of brain tissue in vitro. In cell-attached recordings, removal of extracellular glucose or metabolic inhibition with sodium azide reduced the firing rate of a subpopulation of cells through the activation of a 65 pS channel that was blocked by the sulphonylureas tolbutamide and glibenclamide. 2. In whole-cell patch-clamp recordings, in the absence of ATP in the electrode solution, glucose-receptive neurones gradually hyperpolarized due to the induction of an outward current at -60 mV. This outward current and the resultant hyperpolarization were blocked by the sulphonylureas tolbutamide and glibenclamide. 3. In recordings where the electrode solution contained 4 mM ATP, this outward current was not observed. Under these conditions, 500 microM diazoxide was found to induce an outward current that was blocked by tolbutamide. 4. In cell-attached recordings diazoxide and the active fragment of leptin (leptin 22-56) reduced the firing rate of glucose-receptive neurones by the activation of a channel with similar properties to that induced by removal of extracellular glucose. 5. Reverse transcription followed by the polymerase chain reaction using cytoplasm from single glucose-receptive neurones demonstrated the expression of the ATP-sensitive potassium (KATP) channel subunits Kir6.1 and SUR1 but not Kir6.2 or SUR2. 6. It is concluded that glucose-receptive neurones within the rat ventromedial hypothalamus exhibit a KATP channel current with pharmacological and molecular properties similar to those reported in other tissues.  (+info)

Functional analysis of the promoter of the yeast SNQ2 gene encoding a multidrug resistance transporter that confers the resistance to 4-nitroquinoline N-oxide. (8/7249)

The yeast gene SNQ2, which encodes a multidrug resistance ABC superfamily protein, is required for resistance to the mutagen 4-nitroquinoline N-oxide (4-NQO). The expression of the SNQ2 gene is under the control of a regulatory network that involves the transcription factor Yrr1p, as well as Pdr1p/Pdr3p (Cui et al., Mol. Microbiol., 29, 1307-1315 (1998)). By 5'-deletion analysis of the promoter by using SNQ2-lacZ fusion constructs, four regions: -745 to -639 (region I), -639 to -578 (region II), -548 to -533 (region III) and -533 to -485 (region IV) were found to be important for SNQ2 expression. Genetic analysis suggested that the site in region IV was responsible for the Yrr1p-mediated SNQ2 expression. A consensus motif known for the binding of Pdr1p/Pdr3p (PDRE) was not found in region IV.  (+info)

KAMISAKO, T. and OGAWA, H. (2005), Alteration of the expression of adenosine triphosphate-binding cassette transporters associated with bile acid and cholesterol transport in the rat liver and intestine during cholestasis. Journal of Gastroenterology and Hepatology, 20: 1429-1434. doi: 10.1111/j.1440-1746.2005.03950.x ...
Title: Structure and Function of the Human Breast Cancer Resistance Protein (BCRP/ABCG2). VOLUME: 11 ISSUE: 7. Author(s):Zhanglin Ni, Zsolt Bikadi, Mark F. Rosenberg and Qingcheng Mao. Affiliation:Department of Pharmaceutics,School of Pharmacy, University of Washington, Health Science Building H272, 1959 NE Pacific Street , Seattle, Washington 98195-7610, USA.. Keywords:Breast cancer resistance protein, BCRP, ATP-binding cassette transporter, ABCG2, multidrug resistance, drug disposition, homology model, mutation analysis, BCRP/ABCG2, mitoxantrone-resistant human cancer cell lines, MXR, human placenta, ABCP, nucleotide binding domains, membrane spanning domains, MSDs, affinity constants, SAR, QSAR, flavonoids, tamoxifen analogues, cyclindependent kinase inhibitors, tariquidar analogues, FTC analogues, 2D-QSAR, CoMFA, CoMSIA, 3D-QSAR, MIFs, HEK cells, Pichia pastoris, Lactococcus lactis, fluorescence resonance energy transfer v, MODELLER. Abstract: The human breast cancer resistance protein ...
ABCC4 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (ABCC4), transcript variant 2 - 10 µg - OriGene - cdna clones
TY - JOUR. T1 - A novel mutation in the ABCR gene in four patients with autosomal recessive Stargardt disease. AU - Zhang, Kang. AU - Garibaldi, Daniel C.. AU - Kniazeva, Marina. AU - Albini, Thomas. AU - Chiang, Michael F.. AU - Kerrigan, Michelle. AU - Sunness, Janet S.. AU - Han, Min. AU - Allikmets, Rando. PY - 1999/12/1. Y1 - 1999/12/1. N2 - PURPOSE: To identify additional mutations in the ABCR gene and describe the clinical features of four affected siblings with autosomal recessive Stargardt disease. METHODS: A cohort of eight siblings was identified for study. Four of these individuals were diagnosed with Stargardt disease based on clinical evaluation and fluorescein angiography. Blood samples were obtained from seven of eight siblings, including all those affected. All 50 exons of the ABCR gene were analyzed by single-stranded confirmation polymorphism analysis, followed by direct sequencing of observed variants, to identify mutations in the ABCR gene. RESULTS: We identified a ...
Overexpression of adenosine triphosphate-binding cassette (ABC) transport proteins is emerging seeing that a crucial contributor to anticancer medication level of resistance. assays. eIF4G mRNA degradation was accelerated in cells transfected with miR-503 mimics. Furthermore, it had been demonstrated that eIF4G and ABC translation protein were downregulated in MCF-7/ADR cells after transfection with miR-503 significantly. It was discovered that miR-503 mimics could sensitize the cells to treatment with ADM, TAX and TAM. These findings confirmed for the very first time that eIF4G acted as an integral element in MCF-7/ADR cells, and Riociguat could end up being a competent agent for preventing and reversing multi-drug resistance in breast malignancy. (11) determinded that cisplatin-resistance cells upregulated MRP1 when compared with sensitive MCF-7 cells. The eukaryotic initiation factor (eIF) 4F complex consists of three proteins: cap-binding protein eIF4E, scaffolding protein eIF4G and ...
Title: Interaction of Probenecid with the Breast Cancer Resistance Protein Transporter (BCRP/ABCG2). VOLUME: 3 ISSUE: 4. Author(s):G. Merino, R. Real, A. J. Molina, M. M. Pulido, J. G. Prieto and A. I. Alvarez. Affiliation:University of León,Department of Physiology, Faculty of Veterinary Medicine, Campus deVegazana 24071 León, Spain.. Keywords:Probenecid, Breast Cancer Resistance Protein, P-glycoprotein, transport, inhibition. Abstract: Probenecid is used as a uricosuric agent in the treatment of chronic gout and as an adjunct to enhance antibiotic levels in the blood. For research purposes, it is used as a prototypic inhibitor of organic anion transporters and MRPs, including MPR2. The purpose of this research is to study the interaction of probenecid with two other important transporters of the ATP-binding cassette family, Breast Cancer Resistance Protein (BCRP) and P-glycoprotein. These drug efflux transporters are present in the intestine, liver and other organs, and they affect the ...
The transport of molecules across lipid membranes is an essential function of all living organisms. One of the families of genes that have evolved to carry out this function is that which encodes the ATP-binding cassette proteins. These molecules use active transport to pump specific molecules acros …
During the past years, we and others discovered a series of human ATP-binding cassette (ABC) transporters, now referred to as ABC A-subfamily transporters. Recently, a novel testis-specific ABC A transporter, Abca17, has been cloned in rodent. In this study, we report the identification and characterization of the human ortholog of rodent Abca17. The novel human ABC A-transporter gene on chromosome 16p13.3 is ubiquitously expressed with highest expression in glandular tissues and the heart. The new ABC transporter gene exhibits striking nucleotide sequence homology with the recently cloned mouse (58%) and rat Abca17 (51%), respectively, and is located in the syntenic region of mouse Abca17 indicating that it represents the human ortholog of rodent Abca17. However, unlike in the mouse, the full-length ABCA17 transcript (4.3 kb) contains numerous mutations that preclude its translation into a bona fide ABC transporter protein strongly suggesting that the human ABCA17 gene is a transcribed pseudogene
[51 Pages Report] Check for Discount on ATP Binding Cassette Sub Family A Member 1 (ATP Binding Cassette Transporter 1 or ABC 1 or ATP Binding Cassette 1 or Cholesterol Efflux Regulatory Protein or ABCA1) - Pipeline Review, H1 2016 report by Global Markets Direct. Global Markets Directs, ATP Binding Cassette Sub...
TY - JOUR. T1 - Functions of the breast cancer resistance protein (BCRP/ABCG2) in chemotherapy. AU - Noguchi, Kohji. AU - Katayama, Kazuhiro. AU - Mitsuhashi, Junko. AU - Sugimoto, Yoshikazu. N1 - Funding Information: We apologize that, due to space limitations, we could not cite the excellent work of many investigators. This work was supported by a grant-in-aid for Cancer Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.. PY - 2009/1/31. Y1 - 2009/1/31. N2 - The breast cancer resistance protein, BCRP/ABCG2, is a half-molecule ATP-binding cassette transporter that facilitates the efflux of various anticancer agents from the cell, including 7-ethyl-10-hydroxycamptothecin, topotecan and mitoxantrone. The expression of BCRP can thus confer a multidrug resistance phenotype in cancer cells, and its transporter activity is involved in the in vivo efficacy of chemotherapeutic agents. Thus, the elucidation of the substrate preferences and structural relationships ...
ATP-binding cassette transporters (ABC transporters) are members of a transport system superfamily that is one of the largest and is possibly one of the oldest families with representatives in all extant phyla from prokaryotes to humans. ABC transporters often consist of multiple subunits, one or two of which are transmembrane proteins and one or two of which are membrane-associated ATPases. The ATPase subunits utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to energize the translocation of various substrates across membranes, either for uptake or for export of the substrate. Most but not all uptake systems also have an extracytoplasmic receptor, a solute binding protein. Some homologous ATPases function in non-transport-related processes such as translation of RNA and DNA repair. ABC transporters are considered to be with the ABC superfamily based on the sequence and organization of their ATP-binding cassette (ABC) domains, even though the integral membrane proteins ...
We characterized a new human ATP-binding cassette (ABC) transporter gene that is highly expressed in the placenta. The gene, ABCP, produces two transcripts that differ at the 5′ end and encode the same 655-amino acid protein. The predicted protein is closely related to the Drosophila white and yeast ADP1 genes and is a member of a subfamily that includes several multidrug resistance transporters. ABCP, white, and ADP1 all have a single ATP-binding domain at the NH2 terminus and a single COOH-terminal set of transmembrane segments. ABCP maps to human chromosome 4q22, between the markers D4S2462 and D4S1557, and the murine gene (Abcp) is located on chromosome 6 28-29 cM from the centromere. ABCP defines a new syntenic segment between human chromosome 4 and mouse chromosome 6. The abundant expression of this gene in the placenta suggests that the protein product has an important role in transport of specific molecule(s) into or out of this tissue.. ...
With the well-established link between serum cholesterol levels and cardiovascular disease and the availability of effective cholesterol-lowering drugs, cholesterol screening has rapidly become a routine part of health care. Yet, much remains to be learned about how cholesterol levels are regulated at the cellular level (see the Perspective by Brown et al.). Now, Najafi-Shoushtari et al. (p. 1566, published online 13 May) and Rayner et al. (p. 1570, published online 13 May) have discovered a new molecular player in cholesterol control-a small noncoding RNA that, intriguingly, is embedded within the genes coding for sterol regulatory element-binding proteins (SREBPs), transcription factors already known to regulate cholesterol levels. This microRNA, called miR-33, represses expression of the adenosine triphosphate-binding cassette transporter A1, a protein that regulates synthesis of high-density lipoprotein (HDL, or good cholesterol) and that helps to remove bad cholesterol from the blood. ...
1. Higgins CF. ABC transporters: from microorganisms to man. Annu Rev Cell Biol. 1992;8: 67-113. doi: 10.1146/annurev.cb.08.110192.000435 1282354. 2. Rees DC, Johnson E, Lewinson O. ABC transporters: the power to change. Nat Rev Mol Cell Biol. 2009;10: 218-227. doi: 10.1038/nrm2646 19234479. 3. Dean M, Rzhetsky A, Allikmets R. The human ATP-binding cassette (ABC) transporter superfamily. Genome Res. 2001;11: 1156-1166. doi: 10.1101/gr.184901 11435397. 4. Klein I, Sarkadi B, Varadi A. An inventory of the human ABC proteins. Biochim Biophys Acta-Biomembr. 1999;1461: 237-262. doi: 10.1016/S0005-2736(99)00161-3. 5. Seeger MA, van Veen HW. Molecular basis of multidrug transport by ABC transporters. Biochim Biophys Acta BBA-Proteins Proteomics. 2009;1794: 725-737. doi: 10.1016/j.bbapap.2008.12.004 19135557. 6. Holland IB, A. Blight M. ABC-ATPases, adaptable energy generators fuelling transmembrane movement of a variety of molecules in organisms from bacteria to humans. J Mol Biol. 1999;293: 381-399. ...
With the well-established link between serum cholesterol levels and cardiovascular disease and the availability of effective cholesterol-lowering drugs, cholesterol screening has rapidly become a routine part of health care. Yet, much remains to be learned about how cholesterol levels are regulated at the cellular level (see the Perspective by Brown et al.). Now, Najafi-Shoushtari et al. and Rayner et al. have discovered a new molecular player in cholesterol control-a small noncoding RNA that, intriguingly, is embedded within the genes coding for sterol regulatory element-binding proteins (SREBPs), transcription factors already known to regulate cholesterol levels. This microRNA, called miR-33, represses expression of the adenosine triphosphate-binding cassette transporter A1, a protein that regulates synthesis of high-density lipoprotein (HDL, or good cholesterol) and that helps to remove bad cholesterol from the blood. Reducing the levels of miR-33 in mice boosted serum HDL levels, ...
Membrane transport proteins are known to influence the absorption, distribution, metabolism, excretion and toxicity (ADMET) of drugs. At the onset of this thesis work, only a few structure-activity models, in general describing P-glycoprotein (Pgp/ABCB1) interactions, were developed using small datasets with little structural diversity. In this thesis, drug-transport protein interactions were explored using large, diverse datasets representing the chemical space of orally administered registered drugs. Focus was set on the ATP-binding cassette (ABC) transport proteins expressed in the canalicular membrane of human hepatocytes.. The inhibition of the ABC transport proteins multidrug-resistance associated protein 2 (MRP2/ABCC2) and bile salt export pump (BSEP/ABCB11) was experimentally investigated using membrane vesicles from cells overexpressing the investigated proteins and sandwich cultured human hepatocytes (SCHH). Several previously unknown inhibitors were identified for both of the proteins ...
TY - JOUR. T1 - ATP-binding cassette transporter A1 (ABCA1) R219K (G1051A, rs2230806) polymorphism and serum high-density lipoprotein cholesterol levels in a large Japanese population. T2 - Cross-sectional data from the Daiko study. AU - Mokuno, Junichiro. AU - Hishida, Asahi. AU - Morita, Emi. AU - Sasakabe, Tae. AU - Hattori, Yuta. AU - Suma, Shino. AU - Okada, Rieko. AU - Kawai, Sayo. AU - Naito, Mariko. AU - Wakai, Kenji. PY - 2015/4/11. Y1 - 2015/4/11. N2 - Among polymorphisms in ATP-binding cassette transporter Al (ABCA1) gene, the available evidence demonstrates that the ABCA1 R219K polymorphism (G1051A, rs2230806) K allele is associated with a higher high-density lipoprotein cholesterol (HDL- C) level and may play a protective role against coronary artery disease (CAD) risk in Asians and Caucasians. The findings from many underpowered studies from Asian countries (n=71-597), however, still remain inconsistent. The objective of this study was to overcome the limitations of previous ...
K11085 msbA; ATP-binding cassette, subfamily B, bacterial MsbA [EC:3.6.3.-] K11085 msbA; ATP-binding cassette, subfamily B, bacterial MsbA [EC:3.6.3.-] K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06147 ABCB-BAC; ATP-binding cassette, subfamily B, bacterial K06160 pvdE; putative pyoverdin transport system ATP-binding/permease protein K15738 uup; ABC transport system ATP-binding/permease protein K21397 K21397; ABC transport system ATP-binding/permease protein K21397 K21397; ABC transport system ATP-binding/permease protein K23163 sbp; sulfate/thiosulfate transport system substrate-binding protein K02046 cysU; ...
Introduction Recently, the ATP-binding cassette transporter BCRP1/ABCG2 has been shown to regulate the function and survival of side population cells, which have been identified in various organs including heart and have stem cell properties. In addition, previous studies have revealed that BCRP1/ABCG2 is also expressed in endothelial cells of capillaries and arterioles in heart. This study was performed to clarify the role of BCRP1/ABCG2 in cardiac repair after myocardial infarction (MI).. Methods and Results MI was induced in 8- to 12-week-old wild-type (WT) mice (n=51) and Bcrp1/Abcg2 knock-out (KO) mice (n=60) by ligating the left anterior descending artery. At 28 days after MI, the survival rate was significantly lower in KO mice than in WT mice (28.3% versus 74.5%, p=0.0001). The main cause of death in KO mice was cardiac rupture (CR) (67.4%), whereas CR was observed in only 30.8% among WT mice (p=0.019). Echocardiography showed that ventricular remodeling was more deteriorated in KO mice ...
ABCF3 - ABCF3 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family F (GCN20), member 3 (ABCF3) available for purchase from OriGene - Your Gene Company.
Purpose : The retina specific ATP binding cassette transporter A4 (ABCA4) is necessary for the clearance of all-trans-retinal from photoreceptor cells. Loss of this crucial function results in the accumulation of toxic bisretinoids, primarily N-retinylidene-N-retinylethanolamine (A2E). This ultimately leads to the Stargardt phenotype of increased autofluorescence and progressive RPE and photoreceptor cell loss. Adeno-associated virus (AAV) vectors have proven their utility for efficient gene transfer in the retina to a variety of cell types. However, the ABCA4 coding sequence (cds) of 6.8kb exceeds the payload capacity of a single AAV capsid of 4.8kb by far. AAV dual vectors have been shown to overcome this size restriction by splitting the cds between two vectors and packaging them in separate capsids. After co-infection of a cell the two vectored cDNAs recombine to reconstitute the full length cds. Here we present recent data on the effect of AAV dual vector mediated ABCA4 gene replacement ...
Division of Molecular Biotherapy [Y. I., M. N., K. K., S. T., E. I., Y. S.] and Division of Experimental Chemotherapy [T. T.], Cancer Chemotherapy Center, and Department of Molecular Diagnosis [Y. M.], Cancer Institute, Japanese Foundation for Cancer Research, Tokyo 170-8455, and Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113-0032 [T. T.], ...
Synthesis of carbon-11-labeled tariquidar derivatives as new PET agents for imaging of breast cancer resistance protein (ABCG2). Appl Radiat Isot. 2010 Jun; 68(6):1098-103 ...
Cell Biol Int. 2021 Mar 24. doi: 10.1002/cbin.11598. Online ahead of print.. ABSTRACT. Overexpression of breast cancer resistance protein (BCRP) plays a crucial role in the acquired multidrug resistance (MDR) in breast cancer. The elucidation of molecular events that confer BCRP-mediated MDR is of major therapeutic importance in breast cancer. Epithelial cell adhesion molecule (EpCAM) has been implicated in tumor progression and drug resistance in various types of cancers, including breast cancer. However, the role of EpCAM in BCRP-mediated MDR in breast cancer remains unknown. In the present study, we revealed that EpCAM expression was upregulated in BCRP-overexpressing breast cancer MCF-7/MX cells, and EpCAM knockdown using siRNA reduced BCRP expression and increased the sensitivity of MCF-7/MX cells to mitoxantrone (MX). Epithelial-mesenchymal transition (EMT) promoted BCRP-mediated MDR in breast cancer cells, and EpCAM knockdown partially suppressed EMT progression in MCF-7/MX cells. In ...
hypothetical protein, ABCB1LB, ATP-binding cassette, sub-family B (MDR/TAP), member 1-like B, A306_07528, ABC16, ABC member 16, MDR/TAP subfamily, AS27_06659, AS28_00614, ATP-binding cassette protein B11, ATP-binding cassette, sub-family B (MDR/TAP), member 11, ATP-binding cassette, subfamily B (MDR/TAP), member 11, ATP-binding cassette, sub-family B (MDR/TAP), member 11-like protein, ATP-binding cassette sub-family B member 11, ATP-binding cassette, sub-family B, member 11, bile salt export pump, BRIC2, BSEP, BSEP/SPGP, CB1_000638007, D623_10034923, GW7_06212, H920_16172, I79_001236, Lith1, liver bile salt export pump, M91_01875, M959_07155, MDA_GLEAN10024246, Multidrug resistance protein 1, N301_03105, N302_06788, N303_07198, N305_06591, N306_04080, N307_07545, N308_11810, N309_07944, N312_11735, N321_13718, N327_01303, N328_07355, N329_09470, N331_01374, N332_02914, N333_01536, N334_13094, N336_04014, N340_01262, N341_10800, PAL_GLEAN10025937, PFIC2, PFIC-2, PGY4, progressive familial ...
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specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C3 (ABCC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C3 (ABCC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C3 (ABCC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter C3 (ABCC3) in the samples is then determined by comparing the ...
The ATP-binding cassette transporter A1 (ABCA1) mediates the efflux of cellular unesterified cholesterol and phospholipid to lipid-poor apolipoprotein A-I. Chymase, a protease secreted by mast cells, selectively cleaves pre-β-migrating particles from high density lipoprotein (HDL)3 and reduces the efflux of cholesterol from macrophages. To evaluate whether this effect is the result of reduction of ABCA1-dependent or -independent pathways of cholesterol efflux, in this study we examined the efflux of cholesterol to preparations of chymase-treated HDL3 in two types of cell: 1) in J774 murine macrophages endogenously expressing low levels of scavenger receptor class B, type I (SR-BI), and high levels of ABCA1 upon treatment with cAMP; and 2) in Fu5AH rat hepatoma cells endogenously expressing high levels of the SR-BI and low levels of ABCA1. Treatment of HDL3 with the human chymase resulted in rapid depletion of pre-β-HDL and a concomitant decrease in the efflux of cholesterol and phospholipid ...
ABCA12 - ABCA12 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family A (ABC1), member 12 (ABCA12), transcript variant 2 available for purchase from OriGene - Your Gene Company.
Cholesterol is an essential molecule that mediates a myriad of critical cellular processes, such as signal transduction in eukaryotes, membrane fluidity, and steroidogenesis. As such it is not surprising that cholesterol homeostasis is tightly regulated, striking a precise balance between endogenous synthesis and regulated uptake/efflux to and from extracellular acceptors. In mammalian cells, sterol efflux is a key component of the homeostatic equation and is mediated by members of the ATP binding cassette (ABC) transporter superfamily. ATP-binding cassette (ABC) transporters represent a group of evolutionarily highly conserved cellular transmembrane proteins that mediate the ATP-dependent translocation of substrates across membranes. Members of this superfamily, ABCA1 and ABCG1, are key components of the reverse cholesterol transport pathway. ABCG1 acts in concert with ABCA1 to maximize the removal of excess cholesterol from cells by promoting cholesterol efflux onto mature and nascent HDL particles
S. griseus mutant NP4, which was isolated by UV mutagenesis, showed a bald and wrinkled colony morphology because of ectopic septation in substrate hyphae and subsequent spore formation. The ectopic spores were the same as aerial spores in size, thickness of the spore wall, and shape, as determined by transmission and scanning electron microscopy, and in heat and lysozyme susceptibility. Mutant NP4 also formed abundant spores in liquid medium, whereas the parental strain IFO13350 rarely forms submerged spores under these conditions. The wall of the ectopic spores is supposed to be thicker than those of the submerged spores formed by several Streptomyces spp., including S. griseus B-2682 (32), under specific conditions, because the spores of NP4 were resistant to lysozyme. We therefore assume that both on solid and in liquid medium, mutant NP4 forms two separate cross walls in the vegetative hyphae and matures each compartment into a spore indistinguishable from aerial spores in many aspects, as ...
Active drug efflux by the adenosine triphosphate-binding cassette (ABC) transporter ABCG2 is one of the common mechanisms causing multiple drug resistance in various human cancers. In the intrinsic drug resistance of hepatocellular carcinoma (HCC), the role of ABCG2 is closely associated with side population (SP), a minor subset of cancer stem-like cells with unique capacity to extrude lipophilic dye Hoechst 33342 and many chemotherapeutic agents. In this study, we showed that ABCG2 was intrinsically expressed in a subgroup of HCC tissues and its expression pattern significantly influenced the levels of drug efflux from HCC cell lines. In MHCC-97L HCC cell line with intrinsic ABCG2 expression, we confirmed the importance of SP cells to the drug efflux-related chemotherapy resistance and found that the SP analysis provided an efficient method to evaluate the functional activity of ABCG2 transporter. In this cell line, we discovered that the SP proportion was modulated by the treatments of Akt ...
ATP binding cassette (ABC) transporters mediate vital transport processes in every living cell. ATP hydrolysis, which fuels transport, displays positive cooperativity in numerous ABC transporters. In particular, heterodimeric ABC exporters exhibit pronounced allosteric coupling between a catalytically impaired degenerate site, where nucleotides bind tightly, and a consensus site, at which ATP is hydrolyzed in every transport cycle. Whereas the functional phenomenon of cooperativity is well described, its structural basis remains poorly understood. Here, we present the apo structure of the heterodimeric ABC exporter TM287/288 and compare it to the previously solved structure with adenosine 5-(β,γ-imido)triphosphate (AMP-PNP) bound at the degenerate site. In contrast to other ABC exporter structures, the nucleotide binding domains (NBDs) of TM287/288 remain in molecular contact even in the absence of nucleotides, and the arrangement of the transmembrane domains (TMDs) is not influenced by ...
Mouse Monoclonal Anti-ABCD3 Antibody against Human ATP-binding cassette, sub-family D (ALD), member 3. Validated for Immunofluorescence and Immunohistochemistry
Our previous work shows that the stem cell factor SALL4 plays a central role in embryonic and leukemic stem cells. In this study, we report that SALL4 expression was higher in drug resistant primary acute myeloid leukemic patients than those from drug-responsive cases. In addition, while overexpression of SALL4 led to drug resistance in cell lines, cells with decreased SALL4 expression were more sensitive to drug treatments than the parental cells. This led to our investigation of the implication of SALL4 in drug resistance and its role in side population (SP) cancer stem cells. SALL4 expression was higher in SP cells compared to non-SP cells by 2-4 fold in various malignant hematopoietic cell lines. Knocking down of SALL4 in isolated SP cells resulted in a reduction of SP cells, indicating that SALL4 is required for their self-renewal. The SP phenotype is known to be mediated by members of the ATP-binding cassette (ABC) drug transport protein family, such as ABCG2 and ABCA3. Using ...
Transporter associated with antigen processing (TAP) is a member of the ATP-binding-cassette transporter family. It delivers cytosolic peptides into the endoplasmic reticulum (ER), where they bind to nascent MHC class I molecules. The TAP structure is formed of two proteins: TAP-1 and TAP-2, which have one hydrophobic region and one ATP-binding region each. They assemble into a heterodimer, which results in a four-domain transporter. The TAP transporter is found in the ER lumen associated with the peptide-loading complex (PLC). This complex of β2 microglobulin, calreticulin, ERp57, TAP, tapasin, and MHC class I acts to keep hold of MHC molecules until they have been fully loaded with peptides. TAP-mediated peptide transport is a multistep process. The peptide-binding pocket is formed by TAP-1 and TAP-2. Association with TAP is an ATP-independent event, in a fast bimolecular association step, peptide binds to TAP, followed by a slow isomerisation of the TAP complex. It is suggested that the ...
d-Methionine is an effective methionine source for Escherichia coli (5, 11, 14). The transport system reported to take up d-methionine in E. coli is encoded by the metD locus (11, 12). The system was found to be energized by ATP and regulated by the level of the internal methionine pool (10, 11, 13). The metD locus was mapped between the fhuA (previously called tonA) and the proA loci (12). The specific genes involved in d-methionine transport have not yet been reported.. We have identified the abc-yaeE-yaeC gene cluster (now renamed metNIQ genes) as a likely candidate for the metD locus in the fhuA-proA region. The abc gene was previously found in a search for ABC transporter ATP-binding domains (1). The PROSITE program (6) indicated that the Abc protein harbors an ATP- and GTP-binding site motif A (P-loop) (24) and an ABC transporter family signature (4). Gene yaeE encodes a putative membrane protein with a high sequence similarity to several bacterial amino acid transporters and contains a ...
Human Transporter Database: Comprehensive Knowledge and Discovery Tools in the Human Transporter Genes. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
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TY - JOUR. T1 - Drug efflux by breast cancer resistance protein is a mechanism of resistance to the benzimidazole insulin-like growth factor receptor/insulin receptor inhibitor, BMS-536924. AU - Hou, Xiaonan. AU - Huang, Fei. AU - Carboni, Joan M.. AU - Flatten, Karen. AU - Asmann, Yan. AU - Ten Eyck, Cynthia. AU - Nakanishi, Takeo. AU - Tibodeau, Jennifer D.. AU - Ross, Douglas D.. AU - Gottardis, Marco M.. AU - Erlichman, Charles. AU - Kaufmann, Scott H. AU - Haluska, Paul. PY - 2011/1. Y1 - 2011/1. N2 - Preclinical investigations have identified insulin-like growth factor (IGF) signaling as a key mechanism for cancer growth and resistance to clinically useful therapies in multiple tumor types including breast cancer. Thus, agents targeting and blocking IGF signaling have promise in the treatment of solid tumors. To identify possible mechanisms of resistance to blocking the IGF pathway, we generated a cell line that was resistant to the IGF-1R/InsR benzimidazole inhibitors, BMS-554417 and ...
GF120918 has a high inhibitory effect on P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP). We developed [11C]GF120918 as a positron emissi
Active drug efflux transporters of the ATP binding cassette (ABC)-containing family of proteins have a major impact on the pharmacological behavior of most of the drugs in use today. Pharmacological properties affected by ABC transporters include the oral bioavailability, hepatobiliary, direct intes …
Background: Development of a multidrug resistance (MDR) phenotype to chemotherapy remains a major barrier in the treatment of cancer. Gankyrin (p28, p28GANK or PSMD10) is an oncoprotein overexpressed in different carcinoma cell lines. The aim of this study was to compare Gankyrin expression level in MDR cells (MCF-7/ADR and MCF-7/ MX) and non-MDR counterparts (MCF-7). Methods: Gankyrin, MDR1 (also known as ABCB1; the ATP-binding cassette sub-family B member 1) and ABCG2 (also known as BCRP; the human breast cancer resistance protein) mRNA levels were analyzed by real-time RT-PCR. Western blot analysis was used to detect the protein expression levels of Gankyrin. Results: The PCR results showed that the expression of Gankyrin was significantly lower in the ABCG2 overexpressing cell line MCF-7/MX than in non-resistanct MCF-7 cells. In contrast, there were no significant differences in mRNA expression of Gankyrin in the MDR1 overexpressing cell line MCF-7/ADR in comparison with MCF-7 cells. Similarly,
OR10G4 (olfactory receptor family 10 subfamily G member 4), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
OR10G8 (olfactory receptor family 10 subfamily G member 8), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
1. Jacquemin E. Progres-sive familial intrahepatic cholestasis. Clin Res Hepatol Gastroenterol 2012; 36 (Suppl 1): S26- S35. doi: 10.1016/ S2210-7401(12)70018-9. 2. Strautnieks SS, Bull LN, Knisely AS et al. A gene encod-ing a liver-specific ABC transporter is mutated in progres-sive familial intrahepatic cholestasis. Nat Genet 1998; 20(3): 233- 238. 3. Jansen PL, Mül-ler M. The molecular genetics of familial intrahepatic cholestasis. Gut 2000; 47(1): 1- 5. 4. Gerloff T, Stieger B, Hagenbuch B et al. The sister of P-glycoprotein represents thecanalicular bile salt export pump of mam-malian liver. J Biol Chem 1998; 273(16): 10046- 10050. 5. Davis RA, Miyake JH, Hui TY et al. Regulation of cholesterol-7alpha-hydroxylase: BAREly mis-s-ing SHP. J Lipid Res 2002; 43(4): 533- 543. 6. Thompson R, Strautnieks SS. BSEP: function and role in progres-sive familial intrahepatic cholestasis. Semin Liver Dis 2001; 21(4): 545- 550. 7. Kaliciński PJ, Ismail H, Jankowska I et al. Surgical treatment of ...
TY - JOUR. T1 - Mutations in the canalicular multispecific organic anion transporter (cMOAT) gene, a novel ABC transporter, in patients with hyperbilirubinemia II/Dubin-Johnson syndrome. AU - Wada, Morimasa. AU - Toh, Satoshi. AU - Taniguchi, Ken. AU - Nakamura, Takanori. AU - Uchiumi, Takeshi. AU - Kohno, Kimitoshi. AU - Yoshida, Ichiro. AU - Kimura, Akihiko. AU - Sakisaka, Shotaro. AU - Adachi, Yukihiko. AU - Kuwano, Michihiko. PY - 1998/2. Y1 - 1998/2. N2 - Members of the ATP-binding cassette (ABC) transporter superfamily are mutated to cause diseases that include cystic fibrosis, hyperinsulinemia, adrenoleukodystrophy, Stargardt disease and multidrug resistance. We recently isolated a novel human member of ABC transporter superfamily as the candidate transporter for the glucuronide and glutathione-conjugated antitumor agents, and found it highly homologous to the rat cmoat gene. Consistent with recent findings of defects in the homologous cmoat gene in two rat models of hyperbilirubinemia ...
Progressive familial intrahepatic cholestasis (PFIC) comprises a group of rare cholestatic liver disorders of childhood that could lead to liver cirrhosis. Nowadays, the partial biliary diversion procedure is still a therapeutic option in non-cirrhotic children with PFIC1 or PFIC2 after an ineffective ursodeoxycholic acid (UDCA) therapy. However, the relevant disadvantage of the partial external biliary diversion (PEBD) is that adolescent patients could not accept a permanent stoma. In some of them, despite of good clinical and biochemical results of this procedure, the ileal exclusion (IE) procedure had to be performed many years after PEBD. Our aims were to find the most characteristic early microscopic features of the disease as well as to compare changes in the liver biopsy specimens at the time of diagnosis and long-time (more than 10 years) after a surgical procedure. We examined retrospectively 8 liver biopsies from 4 PFIC2 patients comparing the results from the first biopsies done at ...
Breast cancer resistance protein (BCRP, ABCG2) is a member of ABC (ATP-binding cassette) transporter superfamily that occurs in a variety of tissues including liver and small intestine of animals. As BCRP is involved in drug absorption, distribution, and elimination, modulation of its expression may affect the clinical efficacy of drugs. However, little is known about the effects of coccidiosis or colibacillosis infection on the levels of BCRP expression in chickens. Here, we studied the effect of infection with Escherichia coli (E. coli) or Eimeriida mixture (E. necatrix and E. tenella) on the expression levels of ABCG2 mRNA and BCRP in the different segments of small intestine and liver in chickens. Expression of ABCG2 mRNA or BCRP was detected in the entire small intestine and liver of healthy chickens, and the expression levels in liver and ileum were significantly higher than duodenum and jejunum. Infection with E. coli or Eimeriida mixture resulted in significant decrease in ABCG2 mRNA and ...
Swiss drugmakers Roche and Novartis have provided financial backing French gene therapy start-up Vivet Therapeutics, with the latter raising EUR37.5 million (US$41 million) in an initial financing round.. The funds will be used by Vivet to advance a diversified pipeline of gene therapy programs targeting rare, inherited metabolic diseases, including Wilson Disease, progressive familial intrahepatic cholestasis type 2 (PFIC2), progressive familial intrahepatic cholestasis type 3 (PFIC3) and citrullinemia type I.. Vivet, created last year in Paris with a wholly owned subsidiary in Spain, is focused on developing novel gene therapies for rare, inherited metabolic diseases.. Its lead program VTX801, which is expected to enter clinical testing by the end of 2018, targets a condition called Wilson Disease.. This rare genetic disorder is caused by a defective gene in liver cells encoding the ATP7B protein, which reduces the livers ability to regulate copper levels in the liver and other tissues ...
Looking for online definition of ATP-binding cassette, sub-family B (MDR/TAP), member 10 in the Medical Dictionary? ATP-binding cassette, sub-family B (MDR/TAP), member 10 explanation free. What is ATP-binding cassette, sub-family B (MDR/TAP), member 10? Meaning of ATP-binding cassette, sub-family B (MDR/TAP), member 10 medical term. What does ATP-binding cassette, sub-family B (MDR/TAP), member 10 mean?
Some Candida albicans isolates from AIDS patients with oropharyngeal candidiasis are becoming resistant to the azole antifungal agent fluconazole after prolonged treatment with this compound. Most of the C. albicans isolates resistant to fluconazole fail to accumulate this antifungal agent, and this has been considered a cause of resistance. This phenomenon was shown to be linked to an increase in the amounts of mRNA of a C. albicans ABC (ATP-binding cassette) transporter gene called CDR1 and of a gene conferring benomyl resistance (BENr), the product of which belongs to the class of major facilitator multidrug efflux transporters (D. Sanglard, K. Kuchler, F. Ischer, J. L. Pagani, M. Monod, and J. Bille, Antimicrob. Agents Chemother. 39:2378-2386, 1995). To analyze the roles of these multidrug transporters in the efflux of azole antifungal agents, we constructed C. albicans mutants with single and double deletion mutations of the corresponding genes. The mutants were tested for their ...
Purpose: : To investigate the progression in Stargardt-fundus flavimaculatus (S-FFM). Methods: : A longitudinal study of 31 patients with S-FFM was undertaken. The mean age of our patients at the initial examination was 28.8 years old (11-50) with a mean follow-up interval of 9.3 years (5-11). Electrophysiological tests, including pattern, focal and full-field electroretinogram (ERG) were performed, as was a clinical ophthalmic examination and fundus autofluorescence imaging. The classification which we previously reported was used for assessment; group 1: dysfunction confined to the macula; group 2: macular and generalized cone dysfunction; group 3: macular, generalized cone and generalized rod dysfunction. At the initial examination, there were 13 patients in group 1, 8 patients in group 2, and 10 patients in group 3. Analysis of the ATP-binding cassette transporter gene (ABCA4) was performed in the majority of the cohort. Results: : Three of 13 patients from group 1 progressed to group 2, and ...
Retinoic acids regulate the reverse cholesterol transport by inducing the ATP binding cassette transporter A1 (ABCA1) dependent cholesterol efflux in macrophages, neuronal as well as intestine cells. In the present study, we aim to test the effect of all trans retinoic acid (ATRA) on ABCA1 expression in human CD4+ T cells and the involvement of cholesterol in ATRA mediated anti-HIV effect. Treatment with ATRA dramatically up-regulated ABCA1 expression in CD4+ T cells in a time and dose dependent manner. The expression of ABCA1 paralleled with increased ABCA1-dependent cholesterol efflux. This induction was dependent on T cell receptor (TCR) signaling and ATRA failed to induce ABCA1 expression in resting T cells. Moreover, ATRA and liver X receptor (LXR) agonist-TO-901317 together had synergistic effect on ABCA1 expression as well as cholesterol efflux. Increased ABCA1 expression was associated with lower cellular cholesterol staining. Cells treated with either ATRA or TO-901317 were less vulnerable to
Because little is known about ABC transporter functions in insects, we examined these proteins in T. castaneum, a well-established and powerful genomic insect model that is highly susceptible to systemic RNAi [22]. Bioinformatic analyses revealed that the ABC transporter superfamily comprises 73 genes in T. castaneum, which group into eight subfamilies. With 35 members, subfamily C constitutes the largest ABC transporter subfamily in T. castaneum. Compared with ABCC subfamilies in other insects, TcABCC appears to have undergone a recent expansion on chromosome 5. In insects, ABC transporters have been implicated in insecticide resistance by increasing the efflux capacity for xenobiotics [37]. This suggests the possibility that the known propensity of this species to develop insecticide resistance might be related to the expansion of subfamily C genes. Indeed, extensive forms of gene duplication (or amplification) are frequently observed for genes involved in insecticide detoxification, including ...
Oxidized sterols consumed in the diet or formed on low-density lipoprotein (LDL) are toxic to endothelial cells and macrophages and are thought to have a central role in promoting atherogenesis. The ATP-binding cassette transporter ABCG1 was recently shown to promote efflux of cholesterol from macrophages to HDL. We show that HDL protects macrophages from apoptosis induced by loading with free cholesterol or oxidized LDL (oxLDL). The protective effect of HDL was reduced in Abcg1−/− macrophages, especially after loading with oxidized LDL. Similarly, HDL exerted a protective effect against apoptosis induced by 7-ketocholesterol, the major oxysterol present in oxLDL and atherosclerotic lesions, in Abcg1+/+ but not in Abcg1−/− macrophages. In transfected 293 cells, efflux of 7-ketocholesterol was completely dependent on expression of ABCG1 and presence of HDL in media. In contrast, ABCA1 and apoA-1 did not stimulate efflux of 7-ketocholesterol into media. HDL stimulated efflux of ...
Differential Effects of Chrysin on Nitrofurantoin Pharmacokinetics Mediated by Intestinal Breast Cancer Resistance Protein in Rats and Mice
The LXRs (liver X receptors) (LXRα and LXRβ) are nuclear hormone receptors that are activated by oxysterols, endogenous oxidative metabolites of cholesterol. These receptors regulate an integrated network of genes that control whole body cholesterol and lipid homoeostasis. A brief overview of the mechanism of this regulation by LXRs in the liver, macrophage and intestine will be outlined, followed by data from our recent work demonstrating that LXRα is crucial in maintaining adrenal cholesterol homoeostasis. In the adrenal gland, oxysterols are formed as intermediates in the conversion of cholesterol into steroid hormones and can act as endogenous activators of LXR. We have found using both gain- and loss-of-function models that LXR acts to maintain free cholesterol below toxic levels in the adrenal gland, through the co-ordinated regulation of genes involved in cholesterol efflux [ABCA1 (ATP-binding-cassette transporter A1)], storage (sterol-regulatory-element-binding protein-1c and ...
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein is likely involved in the peroxisomal transport or catabolism of very long chain fatty acids. Defects in the human gene have been identified as the underlying cause of adrenoleukodystrophy, an X-chromosome recessively inherited demyelinating disorder of the nervous system. [provided by RefSeq, Jul 2008 ...
P-glycoprotein (P-gp) is a member of the ATP-Binding Cassette transporter superfamily and mediates transmembrane efflux of many drugs. Since it is involved in multi-drug resistance activity in various cancer cells, the development of P-gp inhibitor is one of the major concerns in anticancer therapy. Human P-gp protein has at least two functional drug binding sites that are called H site and R site, hence it has multi-binding-specificities. Though the amino acid residues that constitute in drug binding pockets have been proposed by previous experimental evidences, the shapes and the binding poses are not revealed clearly yet. In this study, human P-gp structure was built by homology modeling with available crystal structure of mouse P-gp as a template and docking simulations were performed with inhibitors such as verapamil, hoechst33342, and rhodamine123 to construct the interaction between human P-gp and its inhibitors. The docking simulations were performed 500 times for each inhibitor, and
In progressive familial intrahepatic cholestasis type 2 (PFIC-2), severe steatorrhea is often documented. However, pancreatic exocrine secretion has not yet bee
From NCBI Gene:. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein likely plays an important role in peroxisome biogenesis. Mutations have been associated with some forms of Zellweger syndrome, a heterogeneous group of peroxisome assembly disorders. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]. From UniProt: ...
The EGFR pathway has been an attractive target because it is dysregulated in a significant fraction of malignant gliomas through overexpression, amplification, and activating mutations (Rich et al., 2004). Moreover, recent studies have demonstrated that EGFRvIII is required for tumor maintenance in glioma (Mukasa et al., 2010). The EGFR tyrosine kinase inhibitor gefitinib has been evaluated in a number of clinical trials for GBM; however, results have been disappointing (Rich et al., 2004; Lieberman et al., 2004). The failure of gefitinib raises questions pertaining to delivery of drug to its target. Active efflux at the BBB could prevent drugs from attaining therapeutic levels in the brain and is probably one of the main reasons behind resistance to chemotherapy. It has been shown that several other tyrosine kinase inhibitors are avid substrates for P-gp and BCRP and that their brain distribution is limited due to active efflux out of the brain (Dai et al., 2003; Chen et al., 2009; Lagas et ...
Phospho-glycoprotein (P-gP) is a polytopic plasma membrane protein whose overexpression causes multidrug resistance (MDR) responsible for the failure of cancer chemotherapy. P-gp 170 is a member of the ATP-binding cassette (ABC) transporter superfamily and has two potentially interesting regions for drugs interfering with its efflux function, namely the oligosaccharides on the first extracellular loop with unknown function and the two intracellular ATP-binding regions providing the energy for drug efflux function. The polylactoseamine oligosaccharides on the first loop can specifically bind the tomato lectin (TL). The P-gp efflux activities of TL-pre-treated MDR resistant cells were measured in the presence of structurally unrelated resistance modifiers such as phenothiazines, terpenoids and carotenoids. The inhibition of efflux activity was measured via the increased rhodamine uptake by mouse lymphoma cells transfected in human MDR1 gene and in human brain capillary endothelial cells. The ...
The overlap in the membrane localization and substrate specificities of Bcrp and P-gp has suggested their cooperation in the active efflux in the blood-tissue barriers. A considerable increase in the accumulation of common substrates in the brain of Mdr1a/1b(−/−)/Bcrp(−/−) mice compared with that observed in either Mdr1a/1b(−/−) or Bcrp(−/−) mice has been interpreted as synergistic effect. The present study investigated this synergistic effect kinetically by using erlotinib, flavopiridol, and mitoxantrone as test compounds. Because Cisternino et al. (2004) reported an induction of Bcrp mRNA in the brain capillaries from Mdr1a/1b(−/−) mice, quinidine and dantrolene were used as probes for P-gp and Bcrp, respectively, to examine their adaptive regulation in the knockout strain.. Adaptive regulation of P-gp at the BBB and BTB of Bcrp(−/−) mice is negligible because neither the Cbrain/Cplasma nor the Ctestis/Cplasma of quinidine changed in Bcrp(−/−) mice compared with the ...
In this study, we performed a mutation analysis of the ABCR gene of 80 unrelated AMD patients and 100 race and age matched control subjects. Of 51 exons of the ABCR gene, 10 that have been reported to have AMD associated mutations were studied.3 The only missense mutation found was a T1428M mutation in exon 29. Allikmetset al reported that this mutation was found in one of 167 AMD patients, and that no such mutation was found in the general population.3 However, in our series, seven of 80 (8.8%) AMD patients and eight of 100 (8.0%) of the control group did, in fact, showed this mutation (Table 1), which was not a statistically significant difference. Other alterations found were mostly polymorphisms or sequence variations. These were found in exons 23, 41, 43, and did not correspond to the 13 AMD associated mutations reported by Allikmets et al.3Apparently, there is heterogeneity in the ABCR gene. Indeed, all of the AMD and the control subjects in our series showed different nucleotide sequences ...
The ATP-binding cassette transporters P-glycoprotein (ABCB1 MDR1) and multidrug resistance protein 4 (MRP4) efflux irinotecan and its active metabolite SN-38 genotype was a significant covariate for the clearance of both irinotecan Epigallocatechin gallate lactone and SN-38 Epigallocatechin gallate lactone. of the topoisomerase Epigallocatechin gallate I enzyme [2]. SN-38 undergoes glucuronic acid Rabbit polyclonal to TranscriptionfactorSp1. … Continue reading The ATP-binding cassette transporters P-glycoprotein (ABCB1 MDR1) and multidrug resistance protein. ...
AUTOSOMAL RECESSIVE STARGARDT DISEASE (STGD1) is the most common inherited juvenile macular degeneration.1 Most patients develop bilateral loss of vision in childhood or early adulthood. This subtype of Stargardt disease is caused by mutations in the ABCA4 gene, which encodes a retina-specific transporter protein (ABCR) in the rims of rod and cone photoreceptor outer segment discs.2-4 Retinal degeneration in ABCA4-linked Stargardt disease is believed to result from the toxic effects of lipofuscin that accumulates in the retinal pigment epithelium (RPE) and the subsequent degeneration of photoreceptors.5. Light can induce photochemical injury at the ocular fundus. Depending on the level and duration of the irradiance, the primary site of damage can be either the photoreceptors or the RPE.6 In ABCA4-linked retinopathies, products generated by the visual cycle accumulate and contribute to retinal damage via both direct toxic effects and increased photosensitivity. A major fluorophore of lipofuscin, ...
ATP-sensitive potassium (KATP) channels comprise four pore-forming Kir6.2 subunits and four modulatory sulfonylurea receptor (SUR) subunits. The latter belong to the ATP-binding cassette family of transporters. KATP channels are inhibited by ATP (or ADP) binding to Kir6.2 and activated by Mg-nucleotide interactions with SUR. This dual regulation enables the KATP channel to couple the metabolic state of a cell to its electrical excitability and is crucial for the KATP channels role in regulating insulin secretion, cardiac and neuronal excitability, and vascular tone. Here, we review the regulation of the KATP channel by adenine nucleotides and present an equilibrium allosteric model for nucleotide activation and inhibition. The model can account for many experimental observations in the literature and provides testable predictions for future experiments.
Introduction Multidrug resistance (MDR) is a major cause of treatment failure and mortality in cancer patients. Breast cancer is the most prevalent cancer among women and the second leading cause of death in cancer. The most widely used treatment of breast cancer is chemotherapy, while the success of chemotherapy in breast cancer patients is also seriously limited by the development of MDR [1]. One well-known mechanism of MDR is the over-expression of ATP-binding cassette transporters such as multidrug resistance gene 1 (MDR1), multidrug resistance-associated protein 1 (MRP1), Sunitinib lung resistance protein (LRP). and the breast cancer resistance protein (BCRP) [2-7]. P-glycoprotein (P-gp), which is encoded by the MDR1, is the most extensively studied drug transporter. It is an integral membrane glycoprotein with a molecular mass of 170 kDa and has been postulated to function as a pump that removes hydrophobic anticancer agents from drug-resistant cells, thus promoting MDR [8]. The novel gene ...
TY - JOUR. T1 - No association of ABCB1 polymorphisms with drug-refractory epilepsy in a north Indian population. AU - Lakhan, R.. AU - Misra, U. K.. AU - Kalita, J.. AU - Pradhan, S.. AU - Gogtay, N. J.. AU - Singh, M. K.. AU - Mittal, B.. N1 - Funding Information: The study was supported by a grant received from the Department of Biotechnology, Government of India, and a fellowship provided by CSIR, New Delhi, India.. PY - 2009/1. Y1 - 2009/1. N2 - Multiple drug resistance is a common problem in the treatment of epilepsy, and approximately 30% of patients continue to have seizures despite all therapeutic interventions. Among various classes of drug transporters, genetic variants of P-glycoprotein (P-gp) encoded by the ABCB1 (ATP-binding cassette subfamily B member 1) gene have been associated with drug-refractory epilepsy. Our aim was to investigate the effect of the 1236C,T(rs1128503), 2677G,T/A(rs2032582), and 3435C,T(rs1045642) single-nucleotide polymorphisms of ABCB1 (or MDR1) on drug ...
In the study of motor proteins, the molecular mechanism of mechanochemical coupling, as well as the cellular role of these proteins, is an important issue. To assess these questions we introduced cDNA of wild-type and site-directed mutant kinesin heavy chains into fibroblasts, and analyzed the behavior of the recombinant proteins and the mechanisms involved in organelle transports. Overexpression of wild-type kinesin significantly promoted elongation of cellular processes. Wild-type kinesin accumulated at the tips of the long processes, whereas the kinesin mutants, which contained either a T93N- or T93I mutation in the ATP-binding motif, tightly bound to microtubules in the center of the cells. These mutant kinesins could bind to microtubules in vitro, but could not dissociate from them even in the presence of ATP, and did not support microtubule motility in vitro, thereby indicating rigor-type mutations. Retrograde transport from the Golgi apparatus to the endoplasmic reticulum, as well as ...
Mutations in the ATP8B1 gene can cause benign recurrent intrahepatic cholestasis 1 (BRIC1) or progressive familial intrahepatic cholestasis 1 (PFIC1), commonly known as ATP8B1 deficiency. ATP8B1 is an aminophospholipid flippase, maintaining membrane asymmetry. In ATP8B1 deficiency the activity of the bile salt export pump (BSEP) which pumps bile salts out ... read more of the hepatocytes into the bile duct is decreased, leading to accumulation of bile salts and cell damage. However, the mechanism by which ATP8B1 defects causes cholestasis is unknown. Involvement of the nuclear receptor FXR or reduced membrane stability and consequent decreased BSEP activity have been suggested. Proper ATP8B1 folding and association with CDC50A is required for ATP8B1 to exit the ER and traffic to the plasma membrane. It was recently demonstrated that ATP8B1 mutations often result in protein misfolding and subsequent ER retention and protein degradation. Molecular chaperones facilitate protein folding and ...
The clearance of apoptotic cells occurs throughout the lifespan of multicellular organisms and is important for development during embryogenesis, the maintenance of tissue integrity and function, and the resolution of inflammation (deCathelineau and Henson, 2003). Here, we report that macrophage ABCA7 enhances the clearance of apoptotic cells in vitro and in vivo. ABCA7 and LRP1 move to the cell surface after stimulation with C1q or apoptotic cells and localize to membrane ruffles or phagocytic cups, respectively. However, ABCA7 also localizes to phagocytic membranes during FcR-mediated phagocytosis, in which ABCA7 levels are not rate limiting. More important, ABCA7 is required for optimal ligand-induced signaling through LRP1, as shown by C1q-induced ERK phosphorylation and for sustained ERK phosphorylation during phagocytosis of apoptotic cells. Finally, ERK phosphorylation itself is shown to be essential for phagocytosis of apoptotic cells but not for FcR-mediated phagocytosis, suggesting a ...
Introduction: The breast cancer resistance protein BCRP, encoded by ABCG2, is a member of the ABC transporter family and is well-known for its contribution to multi-drug resistance in cancer. Although BCRP expression is altered in various cancer types, its role and function in cancer, independent from drug efflux, remains largely elusive. In this study, we aimed to elucidate regulatory mechanisms of ABCG2 expression and its biological relevance in clear cell renal cell carcinoma (ccRCC) as well as associations with response to sunitinib treatment in patients with metastatic ccRCC.. Experimental Procedures: Data from the ccRCC cohort of The Cancer Genome Atlas (TCGA) was used to assess ABCG2 mRNA expression (n=463), DNA methylation (n=288) and genetic variants present in tumor tissue (n=326) within the ABCG2 gene region. An independent ccRCC cohort of 64 patients was used for the analysis of ABCG2 mRNA and protein expression by TaqMan quantitative real time PCR or immunohistochemical staining of ...
ABC transporters play a key role in protecting the brain parenchyma by exerting their action at the blood-brain barrier (BBB). However, they also block the entry of therapeutic drugs. One of the key transporters playing this role is ABCG2. While other ABC transporters can be studied through PET, no probe exists for imaging ABCG2 function. D-luciferin, the endogenous substrate of fLuc, has been shown to demonstrate decreased bioluminescence in ABCG2-expressing cells, and low brain distribution. We hypothesized that we can image ABCG2 function at the BBB using bioluminescent imaging in transgenic mice expressing fLuc in the brain. In vitro, accumulation of D-luciferin was lowest in cells overexpressing ABCG2, and the accumulation increased when co-administered Ko143, a potent and selective ABCG2 inhibitor. Using a mouse model expressing fLuc behind the GFAP promoter, mainly expressed in astrocytes of the brain, D-luciferin BLI signal was found to be low. However, it increased in a dose-dependent ...
Background. Our aim was to determine whether the common variants within the coding sequence of ABCA1 gene affects low plasma high-density lipoprotein cholesterol (HDL-C) levels in Turkish patients with coronary artery disease (CAD). The study group was composed of 552 CAD patients, of which 251 had HDL-C levels 40 mg/dL. ...
The bile salt export pump (BSEP) is an ABC-transporter expressed at the canalicular membrane of hepatocytes. Its physiological role is to expel bile salts into the canaliculi from where they drain into the bile duct. Inhibition of this transporter may lead to intrahepatic cholestasis. Predictive computational models of BSEP inhibition may allow for fast identification of potentially harmful compounds in large databases. This article presents a predictive in silico model based on physicochemical descriptors that is able to flag compounds as potential BSEP inhibitors. This model was built using a training set of 670 compounds with available BSEP inhibition potencies. It successfully predicted BSEP inhibition for two independent test sets and was in a further step used for a virtual screening experiment. After in vitro testing of selected candidates, a marketed drug, bromocriptin, was identified for the first time as BSEP inhibitor. This demonstrates the usefulness of the model to identify new BSEP ...
BmrA from Bacillus subtilis is a half-size ABC (ATP-binding cassette) transporter involved in multidrug resistance. Although its supramolecular organization has been investigated after reconstitution in a lipid bilayer environment, and shows a dimeric and possibly a tetrameric form, the precise quaternary structure in a detergent-solubilized state has never been addressed. In the present study, BmrA was purified from Escherichia coli membranes using an optimized purification protocol and different detergents. Furthermore, the ATPase activity of BmrA and the quantity of bound lipids and detergent were determined, and the oligomeric state was analysed using SEC (size-exclusion chromatography) and analytical ultracentrifugation. The activity and the quaternary structure of BmrA appeared to be strongly influenced by the type and concentration of the detergent used. SEC data showed that BmrA could be purified in a functional form in 0.05 and 0.01% DDM (n-dodecyl-β-D-maltoside) and was homogeneous ...
The ATP-binding cassette (ABC) transporter ABCB1, encoded by the multidrug resistance gene MDR1, is expressed on brain microvascular endothelium and several types of epithelium, but not on endothelia outside the CNS. It is an essential component of the blood-brain barrier. The aim of this study was to identify cell-specific controls on the transcription of MDR1 in human brain endothelium. Reporter assays identified a region of 500bp around the transcription start site that was optimally active in brain endothelium. Chromatin immunoprecipitation identified Sp3 and TFIID associated with this region and EMSA (electrophoretic mobility shift assays) confirmed that Sp3 binds preferentially to an Sp-target site (GC-box) on the MDR1 promoter in brain endothelium. This result contrasts with findings in other cell types and with the colon carcinoma line Caco-2, in which Sp1 preferentially associates with the MDR1 promoter. Differences in MDR1 transcriptional control between brain endothelium and Caco-2 ...
The ATP-binding cassette (ABC) transporters form one of the largest known protein families, and are widespread in bacteria, archaea, and eukaryotes. They couple ATP hydrolysis to active transport of a wide variety of substrates such as ions, sugars, lipids, sterols, peptides, proteins, and drugs. The structure of a prokaryotic ABC transporter usually consists of three components; typically two integral membrane proteins each having six transmembrane segments, two peripheral proteins that bind and hydrolyze ATP, and a periplasmic (or lipoprotein) substrate-binding protein. Many of the genes for the three components form operons as in fact observed in many bacterial and archaeal genomes. On the other hand, in a typical eukaryotic ABC transporter, the membrane spanning protein and the ATP-binding protein are fused, forming a multi-domain protein with the membrane-spanning domain (MSD) and the nucleotide-binding domain (NBD ...
Expression of the multidrug transporter P-glycoprotein (P-gp) is one of the major causes of multidrug resistance in cancer cells. P-gp is a 170-kDa membrane protein encoded by the MDR1 gene in humans. Based on its sequence and domain organization, P-gp is classified as a member of ATP binding cassette superfamily; it consists of two symmetrical halves, each half containing six transmembrane (TM) domains and a cytoplasmic nucleotide binding domain. Although most members of the ABC transporter family have stringent substrate specificities, P-gp recognizes many compounds, including anthracyclines (e.g., doxorubicin and daunomycin), vinca alkaloids (e.g., vincristine and vinblastine), antibiotics (e.g., actinomycin D), circular and toxic peptides (e.g., valinomycin and gramicidin), and relatively noncytotoxic agents such as verapamil, azidopine, quinidine, and cyclosporin A. These P-gp substrates have no common chemical structure. They are all low-molecular-weight nonanionic hydrophobic or ...
EXCLUSIVE: What if Sex and the Citys Carrie Bradshaw was dying of cancer? That in essence is the premise of the Mexican drama series Terminales, which is being adapted by ABC Family. Lionsgate TV and Kapital Entertainment are producing the U.S.
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Predicted to have ATPase-coupled transmembrane transporter activity and lipid transporter activity. Involved in neutral lipid metabolic process. Localizes to endoplasmic reticulum. Orthologous to human ABCA17P (ATP binding cassette subfamily A member 17, pseudogene ...
This work is the first description of the expression of human BCRP in X. laevis oocytes and demonstrates that this heterologous expression system is a valid model for examining the physical and functional characteristics of this ABC half transporter. X. laevis oocytes injected with BCRP cRNA synthesize a protein with the molecular mass of glycosylated BCRP and the immunological characteristics of native human BCRP protein. Our results further demonstrate that oocytes injected with mutant R482T or wild-type BCRP cRNA express BCRP in the oocyte plasma membrane, as evidenced by confocal immunofluorescence microscopic analysis. Accumulation and efflux assays using various BCRP substrate molecules indicate that the oocyte-expressed BCRP functions in a manner analogous to that observed in mammalian systems. Moreover, the X. laevis model indicates that a mutation of serine at codon 187 in the ABC-signature motif of BCRP is not only devoid of transporter activity but also serves in a manner analogous to ...
Among the various transporter genes identified in the C. albicans genome (7, 8), there are overwhelming clinical and experimental evidences to suggest that out of 28 ABC transporters, only two of its members, CaCdr1p and CaCdr2p, and only CaMdr1p out of a total 95 of members of MFS transporters are major determinants of MDR (18, 23). Therefore, the search for novel and potent modulators which can block and reverse the drug extrusion mediated by these efflux proteins represents an attractive strategy of anti-Candida therapy. While there are several compounds which are shown to reverse MDR mediated by Pgp (human homologue of CaCdr1p and CaCdr2p) and are at various stages of clinical trials, there are not many instances of compounds which could inhibit/reverse drug extrusion mediated by fungal multidrug transporters (12, 14, 22, 31). We have been exploring modulators/inhibitors of MDR pump proteins which could block the efflux of drugs from fungal cells. For example, we have earlier reported that ...
One of the main obstacles in tumor therapy is multiple drug resistance (MDR) and an underlying mechanism of MDR is up-regulation of the transmembrane ATP-binding cassette (ABC) transporter proteins, especially P-glycoprotein (P-gp). In the synergistic treatment of siRNA and anti-cancer drug doxorubicin, it is crucial that both the siRNA and doxorubicin are simultaneously delivered to the tumor cel ...
ABCB10, or ATP binding cassette sub-family B member 10, is a protein localized in the mitochondrial inner membrane. It belongs to the ABC transporter family whose members are proteins that facilitate substrate transport across various biological membranes. It has been found that ABCB10 is required for normal heme biosynthesis during erythroid differentiation and also plays a role in protection against the damage caused by reactive oxygen species (ROS) production. This protective effect exists both in the erythrocyte development and in the heart recovery after the ischemia-reperfusion injury. However, as an ABC transporter, its transported substrates are not known, neither is the mechanism by which ABCB10 plays a role in protection against ROS damage. In this dissertation an 8-azido-ATP photolabeling system is established to study the ATP binding and hydrolysis properties of ABCB10. Using this approach, it is found that the conserved amino acid residues Gly497 and Lys498 in the Walker A motif of ...
FUNCTION: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein is unknown; however, a similar rat protein has been identified as the major canalicular bile salt export pump of liver. [provided by RefSeq, Jul 2008 ...
ATP-binding cassette (ABC) transporters convert chemical energy from ATP hydrolysis to mechanical work for substrate translocation. They function by alternating between two states, exposing the substrate-binding site to either side of the membrane. A key question that remains to be addressed is how substrates initiate the transport cycle. Using x-ray crystallography, we have captured the maltose transporter in an intermediate step between the inward- and outward-facing states. We show that interactions with maltose-bound MBP in the periplasm induce a partial closure of the MalK dimer in the cytoplasm. ATP binding to this conformation then promotes progression to the outward-facing state. These results, interpreted in light of biochemical and functional studies, provide a structural basis to understand allosteric communication in ABC transporters.. ...
Rea is known for his work on vacuolar proton (H+) pumps, ATP-binding cassette (ABC) transporters, and the enzyme phytochelatin ... Rea, P.A. (2007). "Plant ATP-binding cassette transporters". Annual Review of Plant Biology. 58: 347-375. doi:10.1146/annurev. ... an Arabidopsis ATP-binding cassette transporter able to transport glutathione S-conjugates and chlorophyll catabolites: ... an Arabidopsis ATP-binding cassette transporter able to transport glutathione S-conjugates and chlorophyll catabolites: ...
Vasiliou V, Vasiliou K, Nebert DW (April 2009). "Human ATP-binding cassette (ABC) transporter family". Human Genomics. 3 (3): ... ATP-binding cassette sub-family A member 13 also known as ABCA13 is a protein that in humans is encoded by the ABCA13 gene on ... "The human ATP binding cassette gene ABCA13, located on chromosome 7p12.3, encodes a 5058 amino acid protein with an ... It belongs to the wide ATP-binding cassette family of proteins. The protein contains 5058 residues, and is currently the ...
ATP-binding cassette sub-family A member 12 also known as ATP-binding cassette transporter 12 is a protein that in humans is ... Dean M, Rzhetsky A, Allikmets R (2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Res. 11 (7): ... "Entrez Gene: ATP-binding cassette". Y. Ishibashi; A. Kohyama-Koganeya; Y. Hirabayashi (2013). "New insights on glucosylated ... ABCA12 belongs to a group of genes called the ATP-binding cassette family, which makes proteins that transport molecules across ...
ATP-binding cassette transporter GRCh38: Ensembl release 89: ENSG00000172350 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... The protein encoded by this gene is included in the ATP-binding cassette transporter (ABC protein) superfamily. ABC proteins ... Dean M, Rzhetsky A, Allikmets R (Jul 2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Res. 11 (7 ... 2002). "Molecular and cytogenetic characterization of the mouse ATP-binding cassette transporter Abcg4". Gene. 293 (1-2): 67-75 ...
"Characterization and functional analysis of the nucleotide binding fold in human peroxisomal ATP binding cassette transporters ... "ATP binding/hydrolysis by and phosphorylation of peroxisomal ATP-binding cassette proteins PMP70 (ABCD3) and ... The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... Gärtner J, Valle D (1993). "The 70 kDa peroxisomal membrane protein: an ATP-binding cassette transporter protein involved in ...
ATP-binding cassette sub-family D member 2 is a membrane pump/transporter protein that in humans is encoded by the ABCD2 gene. ... ATP-binding cassette transporter ABCD2 has been shown to interact with PEX19. GRCh38: Ensembl release 89: ENSG00000173208 - ... The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... and heterodimerization of peroxisomal ATP-binding cassette half-transporters". J. Biol. Chem. 274 (46): 32738-43. doi:10.1074/ ...
Lr34 encodes an adenosine triphosphate (ATP)-binding cassette (ABC) transporter. The dominant allele that provides disease ... The xa13 gene has a mutated effector-binding element in its promoter that eliminates PthXo1 binding and renders these lines ... Examples: Auxin: binds to receptors that then recruit and degrade repressors of transcriptional activators that stimulate auxin ... Many R genes encode NB-LRR proteins (proteins with nucleotide-binding and leucine-rich repeat domains, also known as NLR ...
Löscher, Wolfgang; Potschka, Heidrun (2005-01-01). "Blood-brain barrier active efflux transporters: ATP-binding cassette gene ... Löscher, Wolfgang; Potschka, Heidrun (2005-05-01). "Role of drug efflux transporters in the brain for drug disposition and ... "Drug resistance in brain diseases and the role of drug efflux transporters". Nature Reviews Neuroscience. 6 (8): 591-602. doi: ...
ATP-binding cassette transporter ABCA1 (member 1 of human transporter sub-family ABCA), also known as the cholesterol efflux ... The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters ... ATP-binding cassette transporter GRCh38: Ensembl release 89: ENSG00000165029 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... Oram JF (August 2002). "ATP-binding cassette transporter A1 and cholesterol trafficking". Current Opinion in Lipidology. 13 (4 ...
This gene is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two ... ABCA9 ATP-binding cassette, sub-family A (ABC1), member 9". Dean M, Rzhetsky A, Allikmets R (2001). "The human ATP-binding ... ATP-binding cassette sub-family A member 9 is a protein that in humans is encoded by the ABCA9 gene. ... cassette (ABC) transporter superfamily". Genome Res. 11 (7): 1156-66. doi:10.1101/gr.184901 (inactive 2021-01-13). PMID ...
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters ... Dean M, Rzhetsky A, Allikmets R (2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Research. 11 (7 ... "ATP-binding cassette transporter ABCB5 gene is expressed with variability in malignant melanoma". Actas Dermo-sifiliograficas. ... "Correlation of induction of ATP binding cassette transporter A5 (ABCA5) and ABCB1 mRNAs with differentiation state of human ...
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... ABCF2 acts as a suppressor of the volume-sensitive outwardly rectifying Cl channel (CLCN3). ATP-binding cassette transporter ... Dean M, Rzhetsky A, Allikmets R (July 2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Research. ... "Entrez Gene: ABCF2 ATP-binding cassette, sub-family F (GCN20), member 2". Ando-Akatsuka Y, Shimizu T, Numata T, Okada Y (2012 ...
The protein belongs to the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules ... Dean, Michael (2002-11-01). "The Human ATP-Binding Cassette (ABC) Transporter Superfamily". National Library of Medicine (US), ... ATP binds at the cytoplasmic side of the protein. Following binding of each, ATP hydrolysis shifts the substrate into a ... ADP is released, and a new molecule of ATP binds to the secondary ATP-binding site. Hydrolysis and release of ADP and a ...
ATP-binding cassette transporter sub-family C member 11, also MRP8 (Multidrug Resistance-Related Protein 8) is a membrane ... ATP-binding cassette transporter Body odor GRCh38: Ensembl release 89: ENSG00000121270 - Ensembl, May 2017 "Human PubMed ... Dean M, Rzhetsky A, Allikmets R (Jul 2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Research. 11 ... The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ...
This gene is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two ATP- ... Dean M, Rzhetsky A, Allikmets R (Jul 2001). "The human ATP-binding cassette (ABC) transporter superfamily". Genome Res. 11 (7 ... "Two new genes from the human ATP-binding cassette transporter superfamily, ABCC11 and ABCC12, tandemly duplicated on chromosome ... "Multiple splicing variants of two new human ATP-binding cassette transporters, ABCC11 and ABCC12". Biochem. Biophys. Res. ...
"Membrane topology distinguishes a subfamily of the ATP-binding cassette (ABC) transporters". FEBS Lett. 402 (1): 1-3. doi: ... In enzymology, a channel-conductance-controlling ATPase (EC 3.6.3.49) is an enzyme that catalyzes the chemical reaction ATP + ... The systematic name of this enzyme class is ATP phosphohydrolase (channel-conductance-controlling). As of late 2007, two ... H2O ⇌ {\displaystyle \rightleftharpoons } ADP + phosphate Thus, the two substrates of this enzyme are ATP and H2O, whereas its ...
One example is ATP-binding cassette (ABC) transporter families. P-glycoproteins (PGPs) are part of this family and substrates ... In general, drug resistance can develop in four different ways: 1) the drug does not bind to the target due to target changes, ... MLs bind irreversible to Glutmate gated chloride ion (GluCl) channels, leading to hyperpolarisation. Pharyngeal and somatic ... This includes transporters which are part of the xenobiotic metabolism by absorbing, distributing and eliminating external ...
MRP2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across ... ATP-binding cassette transporter GRCh38: Ensembl release 89: ENSG00000023839 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... "Entrez Gene: ABCC2 ATP-binding cassette, sub-family C (CFTR/MRP), member 2". Sekine T, Miyazaki H, Endou H (February 2006). " ... or ATP-binding cassette sub-family C member 2 (ABCC2) is a protein that in humans is encoded by the ABCC2 gene. ...
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... Kajinami K, Brousseau ME, Nartsupha C, Ordovas JM, Schaefer EJ (Apr 2004). "ATP binding cassette transporter G5 and G8 ... "Mutations in the human ATP-binding cassette transporters ABCG5 and ABCG8 in sitosterolemia". Human Mutation. 20 (2): 151. doi: ... "Entrez Gene: ABCG5 ATP-binding cassette, sub-family G (WHITE), member 5 (sterolin 1)". Schmitz G, Langmann T, Heimerl S (Oct ...
It induces the expression of ATP-binding cassette transporter, which can export toxic substances out of the cell, allowing ... Both Pdr1p and Pdr3p regulate Pdr5p, which is an ATP-binding cassette transporter. A single amino acid substitution mutation, ... Pdr1p binds to sequence 5'-TCCGYGGR-3' of PDRE, which is located within the promoter sequences of its target genes. 218 genes ... It binds to DNA sequences that contain certain motifs called pleiotropic drug response element (PDRE). Pdr1p is encoded by a ...
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... membrane-spanning domain that sets it apart from other transporters within the ATP-binding cassette family of transporters. The ... ATP-binding cassette transporter P-glycoprotein (Multidrug resistance protein- MDR1). Not to be confused with Multidrug ... ATP-binding Cassette (ABC) Transporter". Journal of Biological Chemistry. 289 (45): 30880-30888. doi:10.1074/jbc.R114.609248. ...
The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters ... transporter activity. • ATPase activity. • GO:0001948 protein binding. • ATP binding. • ATPase-coupled transmembrane ... ATP-binding cassette super-family G member 2 is a protein that in humans is encoded by the ABCG2 gene.[5][6] ABCG2 has also ... ABCG2 ATP-binding cassette, sub-family G (WHITE), member 2".. *^ a b c d Vlaming ML, Lagas JS, Schinkel AH (January 2009). " ...
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... toxin transporter activity. • protein binding. • protein heterodimerization activity. • phospholipid binding. • ATP binding. • ... "Expression of the ATP-binding cassette transporter gene ABCG1 (ABC8) in Tangier disease". Biochemical and Biophysical Research ... ATP-binding cassette sub-family G member 1 is a protein that in humans is encoded by the ABCG1 gene.[5][6][7] It is a homolog ...
Petry, Frauke (2004). Charakterisierung eines neuen ATP-binding-cassette Transporters aus der ABCA-Subfamilie (PDF) (in German ...
2004). "ATP binding cassette transporter G5 and G8 genotypes and plasma lipoprotein levels before and after treatment with ... The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... 2002). "Mutations in the human ATP-binding cassette transporters ABCG5 and ABCG8 in sitosterolemia". Hum. Mutat. 20 (2): 151. ... 2002). "Catalog of 605 single-nucleotide polymorphisms (SNPs) among 13 genes encoding human ATP-binding cassette transporters: ...
ATP binding cassette (ABC) type transporters are common to the three domains of life. Some secreted proteins are translocated ... The process begins as a leader sequence on the protein to be secreted is recognized by HlyA and binds HlyB on the membrane. ... Thanassi DG, Stathopoulos C, Karkal A, Li H (2005). "Protein secretion in the absence of ATP: the autotransporter, two-partner ... This signal sequence is extremely specific for the ABC transporter. The HlyAB complex stimulates HlyD which begins to uncoil ...
The complex is a member of the ATP-binding cassette (ABC) transporter protein family. The transporter contains three protein ... "Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli". Journal of Biological ... ZnuABC is a high-affinity transporter specialized for transporting zinc ions as part of a system for metal ion homeostasis in ... ZnuC, an ATPase responsible for coupling ion transport to ATP hydrolysis. The expression of ZnuABC is regulated by the zinc ...
Putative ATP-binding cassette transporter sub-family C member 13 is a protein that is not present in humans. In humans, ABCC13 ... ATP-binding cassette transporter ABCC13+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) ... This gene is a member of the superfamily of genes encoding ATP-binding cassette (ABC) transporters. ABC proteins transport ... Dean M, Annilo T (2005). "Evolution of the ATP-binding cassette (ABC) transporter superfamily in vertebrates". Annual Review of ...
Her research focuses on elucidating the structure and function of ATP-binding cassette (ABC) transporters. Chen was born in ... where she started studying the ATP binding cassette transporters In 2002, Chen became an assistant professor at Purdue ... "A Tweezers-like Motion of the ATP-Binding Cassette Dimer in an ABC Transport Cycle". Molecular Cell. 12 (3): 651-661. doi: ... Her work on ABC transporters includes investigating their role in resistance to chemotherapy drugs; antigen presentation in ...
"A Plant Plasma Membrane ATP Binding Cassette-Type Transporter Is Involved in Antifungal Terpenoid Secretion". Plant Cell. 13 (5 ...
ATP-binding cassette transporter. *ABCA1. *ABCG5. *ABCG8. This article on a gene on human chromosome 19 is a stub. You can help ... lipid transporter activity. Cellular component. • high-density lipoprotein particle. • extracellular region. • very-low-density ...
The Human ATP-Binding Cassette (ABC) Transporter Superfamily. Bethesda (MD):National Library of Medicine (US), NCBI; 2002 ... Matsson, P. ATP-Binding Cassette Efflux Transporters and Passive Membrane Permeability in Drug Absorption and Disposition. Acta ... Az ABC-transzporterek (ATP-binding casette transporters ATP-kötő kazetta transzporterek) az egyik legnagyobb és legősibb ... A szupercsalád tagjait az ATP-kötő doménjük (vagy doménjeik) -más néven NBF (nucleotide binding fold)- aminosavsorrendje és ...
ATP binding cassette (ABC) type transporters are common to all the three domains of life. The Sec system constituting the Sec Y ... The process begins as a leader sequence HlyA is recognized and binds HlyB on the membrane. This signal sequence is extremely ... Thanassi DG, Stathopoulos C, Karkal A, Li H (2005). "Protein secretion in the absence of ATP: the autotransporter, two-partner ... direct translocation of proteins across the plasma membrane likely through membrane transporters, 2) blebbing, 3) lysosomal ...
ATP-dependent association of the nucleotide binding cassettes during the catalytic cycle of ATP-binding cassette transporters ... ATP-binding cassette transporters (ABC transporters) are members of a transport system superfamily that is one of the largest ... Each member of the ABCF subgroup consist of a pair of ATP binding domains. Six half transporters with ATP binding sites on the ... Dimer formation of the two ABC domains of transporters requires ATP binding. It is generally observed that the ATP bound state ...
... s express transporters of the ATP-binding cassette family that actively pump a diversity of organic molecules ... out of the cell.[78] Many pharmaceuticals are exported by these transporters conferring multidrug resistance onto the cell. ...
transportador de casete de unión ao ATP. (ATP-binding cassette transporter). ABCA1 · ABCG5 · ABCG8 ... Weisgraber KH, Innerarity TL, Mahley RW (Mar 1982). "Abnormal lipoprotein receptor-binding activity of the human E apoprotein ... "Apolipoprotein E: high-avidity binding to beta-amyloid and increased frequency of type 4 allele in late-onset familial ...
... a member of the ATP-binding cassette transporter superfamily. Whether MRP4 is the only transporter releasing prostaglandins ... To treat egg binding in small birds[20]. *As an ingredient in eyelash and eyebrow growth beauty products due to side effects ... The release of prostaglandin has now also been shown to be mediated by a specific transporter, namely the multidrug resistance ... The discovery of the prostaglandin transporter (PGT, SLCO2A1), which mediates the cellular uptake of prostaglandin, ...
The protein belongs to the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules ... nucleotide binding. • transporter activity. • ATPase activity. • protein binding. • hydrolase activity. • ATP binding. • ... ATP binds at the cytoplasmic side of the protein. Following binding of each, ATP hydrolysis shifts the substrate into a ... ADP is released, and a new molecule of ATP binds to the secondary ATP-binding site. Hydrolysis and release of ADP and a ...
ATP binding cassette transporterEdit. Transporting chemicals out of a cell against a gradient is often associated with ATP ... Transport is mediated by ATP binding cassette transporters. The human genome encodes 48 ABC transporters, that are used for ... Binding of metal cations to ATPEdit. Being polyanionic and featuring a potentially chelatable polyphosphate group, ATP binds ... The protein has two binding sites for ATP - the active site is accessible in either protein conformation, but ATP binding to ...
... an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct ...
ATP-binding cassette transporter. *ABCA1. *ABCG5. *ABCG8. *v. *t. *e. Lipids: phospholipids ... The acetyl-CoA is then ultimately converted into ATP, CO2, and H2O using the citric acid cycle and the electron transport chain ... The energy yield of the complete oxidation of the fatty acid palmitate is 106 ATP.[97] Unsaturated and odd-chain fatty acids ... Glycolysis → Pyruvate decarboxylation → Citric acid cycle → Oxidative phosphorylation (electron transport chain + ATP synthase) ...
... a member of the ATP-binding cassette transporter superfamily. Whether MRP4 is the only transporter releasing prostaglandins ... For example, 1 g of glycogen can bind approximately 2 g of water, which translates to 1.33 kcal/g (4 kcal/3 g). This means that ... Once inside the cell long-chain-fatty-acid-CoA ligase catalyzes the reaction between a fatty acid molecule with ATP (which is ... The energy released in this process is captured in the form of 1 GTP and 11 ATP molecules per acetyl-CoA molecule oxidized. ...
ATP-binding cassette transporter 12 층판상 어린선, type 3 604777 CYP4F22 Cytochrome P450, subfamily 4F, polypeptide 22 ... Emopamil binding protein Ichthyosis follicularis with alopecia and photophobia syndrome 308205 MBTPS2 Membrane-bound ... ATP-binding cassette transporter 12 Ichthyosis bullosa of Siemens 146800 KRT2 Keratin 2A ...
The ATP-binding cassette superfamily (ABC). *The small multidrug resistance family (SMR) ... peptide transporters (PEPTs), and Na+ phosphate transporters (NPTs). These transporters are distributed along particular ... Of these, only the ABC superfamily are primary transporters, the rest being secondary transporters utilizing proton or sodium ... Bacterial efflux transporters are classified into five major superfamilies, based on their amino acid sequence and the energy ...
Fourier Transform Infrared Spectroscopy of the Nucleotide-binding Domain from the ATP-binding Cassette Transporter MsbA: ATP ... Zharova, T.V, & Vinogradov, Proton-Translocating ATP-synthase of Paracoccus denitrificans: ATP- Hydrolytic Activity. ... the hydrolysis of ATP has the effect of lowering the pH of the reaction medium. Under certain conditions, high levels of ATP ... "ATP: Adenosine Triphosphate". cnx.org. Retrieved 2018-05-16.. *^ Wackerhage, H.; Hoffmann, U.; Essfeld, D.; Leyk, D.; Mueller, ...
ATP-binding cassette transporter. *ABCA1. *ABCG5. *ABCG8. Retrieved from "https://en.wikipedia.org/w/index.php?title= ... lipid binding. Cellular component. • chylomicron. • very-low-density lipoprotein particle. • spherical high-density lipoprotein ... phospholipase binding. • protein homodimerization activity. • lipoprotein lipase activator activity. • phospholipase activator ...
... which is a membrane transporter from the large ATP-binding cassette transporter family) influences the disease course is ... mutations in the MRP6 gene encoding a transmembrane ATP-binding cassette (ABC) transporter". Proc. Natl. Acad. Sci. U.S.A. 97 ( ... "Mutations in a gene encoding an ABC transporter cause pseudoxanthoma elasticum". Nat. Genet. 25 (2): 223-7. doi:10.1038/76102 ... "Mutations of the gene encoding the transmembrane transporter protein ABC-C6 cause pseudoxanthoma elasticum". J. Mol. Med. 78 ...
transportador de casete de unión ao ATP. (ATP-binding cassette transporter). ABCA1 · ABCG5 · ABCG8 ... Huang CX, Zhang YL (2013). "The target of regulating the ATP-binding cassette A1 protein (ABCA1): promoting ABCA1-mediated ... capacidade de captar colesterol procedente do interior das células ao interaccionar co transportador de casete de unión ao ATP ...
ATP-dependent association of the nucleotide binding cassettes during the catalytic cycle of ATP-binding cassette transporters" ... The ATP binding cassette multidrug transporter LmrA and lipid transporter MsbA have overlapping substrate specificities". J. ... Human ATP-Binding Cassette Transporters *^ Ueda, K.; DP, Clark; CJ, Chen; IB, Roninson; Gottesman, Michael M.; I, Pastan (1987 ... Rea, P. A. (2007). „Plant ATP-binding cassette transporters". Annu Rev Plant Biol. 58: 347-75. PMID 17263663. doi:10.1146/ ...
The TFIID is the first component to bind to DNA due to binding of TBP, while TFIIH is the last component to be recruited. In ... G-Less Cassette transcription assay: measures promoter strength. *Run-off transcription assay: identifies transcription start ... Goodrich JA, Tjian R (April 1994). "Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA ... TFIIH has been recently reported as a glucose conjugate for targeting hypoxic cancer cells with increased glucose transporter ...
The ATP-binding cassette superfamily (ABC)[4]. *The small multidrug resistance family (SMR)[4] ... peptide transporters (PEPTs), and Na+ phosphate transporters (NPTs). These transporters are distributed along particular ... The duct is also bound to two other proteins: a periplasmic membrane protein and an integral membrane transporter. The ... Of these, only the ABC superfamily are primary transporters, the rest being secondary transporters utilizing proton or sodium ...
The Duffy binding protein found on Plasmodia, the one and only invasion ligand for DARC, does not bind to Saimiri erythrocytes ... anion transporter (organic) - molecules that play an essential role in the distribution and excretion of numerous endogenous ... ATP - (Adenosine triphosphate) - an organic molecule containing high energy phosphate bonds used to transport energy within a ... Lemaitre B, Nicolas E, Michaut L, Reichhart JM, Hoffmann JA (1996). "The dorsoventral regulatory gene cassette spätzle/Toll/ ...
This membrane-associated protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins ... ATP-binding cassette, sub-family B member 11 also known as ABCB11 is a protein which in humans is encoded by the ABCB11 gene. ... The product of the ABCB11 gene is an ABC transporter named BSEP (Bile Salt Export Pump), or sPgp (sister of P-glycoprotein). ... In humans, the activity of this transporter is the major determinant of bile formation and bile flow. ABCB11 is a gene ...
SUR2A, the adenosine triphosphate (ATP)-binding cassette transporter of the IK(ATP) channel.[6] ...
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters ... 2002). "Catalog of 605 single-nucleotide polymorphisms (SNPs) among 13 genes encoding human ATP-binding cassette transporters: ... ATP-binding cassette sub-family A member 8 is a protein that in humans is encoded by the ABCA8 gene. ... "Entrez Gene: ABCA8 ATP-binding cassette, sub-family A (ABC1), member 8". CS1 maint: discouraged parameter (link) Gerhard DS, ...
Up to 19 types of permease exist to transport various carbohydrates with 13 being ATP-binding cassette transporters. B. longum ... The persistence of B. longum in the gut is attributed to the glycoprotein-binding fimbriae structures and bacterial ...
"Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9)". J ... The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters ... ATP-binding cassette transporter GRCh38: Ensembl release 89: ENSG00000150967 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... ATP-binding cassette sub-family B member 9 is a protein that in humans is encoded by the ABCB9 gene. ...
ATP-dependent association of the nucleotide binding cassettes during the catalytic cycle of ATP-binding cassette transporters ... ATP-binding cassette transporters (ABC transporters) are members of a transport system superfamily that is one of the largest ... Each member of the ABCF subgroup consist of a pair of ATP binding domains. Six half transporters with ATP binding sites on the ... Dimer formation of the two ABC domains of transporters requires ATP binding. It is generally observed that the ATP bound state ...
One of the families of genes that have evolved to carry out this function is that which encodes the ATP-binding cassette ... Evolution of ATP-binding cassette transporter genes Curr Opin Genet Dev. 1995 Dec;5(6):779-85. doi: 10.1016/0959-437x(95)80011- ... One of the families of genes that have evolved to carry out this function is that which encodes the ATP-binding cassette ...
... transporter facilitates the efflux of free cholesterol and phospholipid onto high density lipoprotein (HDL) particles, mainly ... The ATP binding cassette A1 (ABCA1) transporter facilitates the efflux of free cholesterol and phospholipid onto high density ... Bodzioch M, Orso E, Klucken J et al (1999) The gene encoding ATP-binding cassette transporter 1 is mutated in Tangier disease. ... Langmann T, Klucken J, Reil M, Liebisch U et al (1999) Molecular cloning of the human ATP binding cassette transporter 1 (hABC1 ...
ATP-binding cassette transporter G1 deficiency dysregulates host defense in the lung.. Draper DW1, Madenspacher JH, Dixon D, ... Mice with genetic deletion of the cholesterol efflux transporter, ATP-binding cassette (ABC) G1, have pulmonary lipidosis and ... ATP-binding Cassette Transporter G1 Deficiency Dysregulates Host Defense in the Lung ... ATP-binding Cassette Transporter G1 Deficiency Dysregulates Host Defense in the Lung ...
Pharmacological properties affected by ABC transporters include the oral bioavailability, hepatobiliary, direct intes … ... Active drug efflux transporters of the ATP binding cassette (ABC)-containing family of proteins have a major impact on the ... Mammalian drug efflux transporters of the ATP binding cassette (ABC) family: an overview Adv Drug Deliv Rev. 2003 Jan 21;55(1): ... Active drug efflux transporters of the ATP binding cassette (ABC)-containing family of proteins have a major impact on the ...
The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter. Elizabeth L. Borths, Kaspar ... Bacterial binding protein-dependent ATP binding cassette (ABC) transporters facilitate uptake of essential nutrients. The ... The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter ... The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter ...
ATP Binding Cassette (ABC) Transporters. Protein: Transporter associated with antigen processing (TAP) bound to ICP47. ... ATP Binding Cassette (ABC) Transporters. Protein: Transporter associated with antigen processing (TAP) bound to ICP47. ... The transporter associated with antigen processing (TAP) is an ATP-binding cassette (ABC) transporter essential to cellular ... The transporter associated with antigen processing (TAP) is an ATP-binding cassette (ABC) transporter essential to cellular ...
... binding cassette(ABC) genes encode transporters that are expressed on the cell membrane or organelle membranes and are involved ... 48 Human ATPBinding Cassette Transporters. Part of a transporter gene website with links to other websites, review articles ... The Human ATPBinding cassette (ABC) transporter superfamily. This is an online monograph on ABC genes with links to other ... The adenosine triphosphate (ATP)‐binding cassette (ABC) genes encode transporters that are expressed on the cell membrane or ...
Mechanism of Coupling of Transport to Hydrolysis in Bacterial ATP-Binding Cassette Transporters. Amy L. Davidson ... Mechanism of Coupling of Transport to Hydrolysis in Bacterial ATP-Binding Cassette Transporters ... Mechanism of Coupling of Transport to Hydrolysis in Bacterial ATP-Binding Cassette Transporters ... Mechanism of Coupling of Transport to Hydrolysis in Bacterial ATP-Binding Cassette Transporters ...
Characterization of the structural requirements for assembly and nucleotide binding of an ATP-binding cassette transporter. The ... Scheme for ATP hydrolysis and vanadate inhibition. In this scheme for ATP hydrolysis by MalFGK2, ATP binding (step 1) and ATP ... during ATP hydrolysis. Binding of both ATP and MBP (E) to FGK will trigger ATP hydrolysis. E becomes more tightly bound to FGK ... Structure of MsbA from E. coli: a homolog of the multidrug resistance ATP binding cassette (ABC) transporters. Science 293:1793 ...
These results provide a basis for further research on ABC transporters involved in detoxification in N. lugens, and for a more ... In addition, ABCG12, which belongs to the ABC transporter G subfamily, was significantly upregulated after exposure to ... transporter superfamily is one of the largest groups of proteins and plays a non-negligible role in phase III of the ... we identified 32 ABC transporters, which are grouped into eight subfamilies (ABCA-H) based on phylogenetic analysis. The ...
13C backbone resonances of the second periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter ... ATP induces conformational changes of periplasmic loop regions of the maltose ATP-binding cassette transporter. J Biol Chem 281 ... NMR assignments of the periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter. ... 13C backbone resonances of the second periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter ...
Most of the protein sequence features involved in binding ATP are conserved, with two exceptions: chicken TAP1 has a glycine in ... Isolation of transporter associated with antigen processing genes, TAP1 and TAP2, from the horned shark Heterodontus francisci. ... Isolation of transporter associated with antigen processing genes, TAP1 and TAP2, from the horned shark Heterodontus francisci ... Experiments performed with BMs from transporter associated with antigen processing (TAP1) knock-out mice demonstrated that the ...
Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily.. ... STE6, the yeast a-factor transporter, is a member of the ATP binding cassette protein superfamily, which also includes the ... and to examine the functional significance of residues conserved among members of the ATP binding cassette superfamily, we ... These proteins contain two homologous halves, each with six membrane spanning segments and a predicted ATP nucleotide binding ...
Structure of the Staphylococcus aureus putative ATPase subunit of an ATP-binding cassette (ABC) transporter. *DOI: 10.2210/ ... Crystal structure of the Staphylococcus aureus putative ATPase subunit of an ATP-binding cassette (ABC) transporter. McGrath, T ... ABC transporter, ATP-binding protein. A, B. 279. Staphylococcus aureus. Mutation(s): 10 EC: 3.6.3.34. ...
Adipose Tissue ATP Binding Cassette Transporter A1 Contributes to High-Density Lipoprotein Biogenesis In Vivo. Soonkyu Chung, ... Adipose Tissue ATP Binding Cassette Transporter A1 Contributes to High-Density Lipoprotein Biogenesis In Vivo ... Adipose Tissue ATP Binding Cassette Transporter A1 Contributes to High-Density Lipoprotein Biogenesis In Vivo ... Adipose Tissue ATP Binding Cassette Transporter A1 Contributes to High-Density Lipoprotein Biogenesis In Vivo ...
... Pedersen, Jenny M. Uppsala University, ... 2. Identification of novel specific and general inhibitors of the three major human ATP-binding cassette transporters P-gp, ... 1. Prediction and identification of drug interactions with the human ATP-binding cassette transporter multidrug-resistance ... Identification of novel specific and general inhibitors of the three major human ATP-binding cassette transporters P-gp, BCRP ...
Metabolism of ATP-binding cassette drug transporter inhibitors: complicating factor for multidrug resistance.. Publication ... ATP-Binding Cassette Transporters · Biological Transport · Cyclosporins · Dibenzocycloheptenes · Drug Design · Drug Resistance ... Membrane transport proteins belonging to the ATP-binding cassette (ABC) family of transport proteins play a central role in the ... Inhibitors of these transporters are thus potentially useful tools to reverse this transporter-mediated cellular resistance to ...
Iron-regulated vacuolar ABC-type transporter which may be responsible for mobilizing stored iron from the vacuole to the ... sp,Q9P5N0,ABC3_SCHPO ATP-binding cassette transporter abc3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN= ... ATP-binding, Nucleotide-binding. Enzyme and pathway databases. Reactome - a knowledgebase of biological pathways and processes ... bind target=_top>More...,/a>,/p>Nucleotide bindingi. 614 - 621. ATP 1PROSITE-ProRule annotation. ,p>Manual validated ...
Novel Insights into Targeting ATP-Binding Cassette Transporters for Antitumor Therapy. Author(s): L. Gatti, G. Cossa, G. L. ... Abstract: ATP-binding cassette (ABC) transporters are a large family of proteins implicated in physiological cellular functions ... ATP-binding cassette (ABC) transporters are a large family of proteins implicated in physiological cellular functions. Selected ... Title: Novel Insights into Targeting ATP-Binding Cassette Transporters for Antitumor Therapy ...
... *Authors: *Ting Xiong ... ATP-binding cassette transporter A1 (ABCA1) has been found to mediate the transfer of cellular cholesterol across the plasma ... Xiong, T., Xu, G., Huang, X., Lu, K., Xie, W., Yin, K., Tu, J.ATPbinding cassette transporter A1: A promising therapy target ... Xiong, T., Xu, G., Huang, X., Lu, K., Xie, W., Yin, K., Tu, J.ATPbinding cassette transporter A1: A promising therapy target ...
ATP binding cassette (ABC) transporters are ATP-dependent membrane proteins predominantly expressed in excretory organs, such ... The Role of ATP Binding Cassette Transporters in Tissue Defense and Organ Regeneration. Miriam Huls, Frans G. M. Russel and ... The Role of ATP Binding Cassette Transporters in Tissue Defense and Organ Regeneration. Miriam Huls, Frans G. M. Russel and ... The Role of ATP Binding Cassette Transporters in Tissue Defense and Organ Regeneration. Miriam Huls, Frans G. M. Russel and ...
Although ATP-binding cassette transporter A1 (ABCA1) is well known for its role in cholesterol efflux and HDL formation, it is ... The ATP-Binding Cassette Transporter A1 R230C Variant Affects HDL Cholesterol Levels and BMI in the Mexican Population. ... The ATP-Binding Cassette Transporter A1 R230C Variant Affects HDL Cholesterol Levels and BMI in the Mexican Population ... The ATP-Binding Cassette Transporter A1 R230C Variant Affects HDL Cholesterol Levels and BMI in the Mexican Population ...
Introduction Recently, the ATP-binding cassette transporter BCRP1/ABCG2 has been shown to regulate the function and survival of ... Abstract 5056: Genetic Disruption of the ATP-binding Cassette Transporter BCRP1/ABCG2 Impairs Cardiac Repair After Myocardial ... Abstract 5056: Genetic Disruption of the ATP-binding Cassette Transporter BCRP1/ABCG2 Impairs Cardiac Repair After Myocardial ... Abstract 5056: Genetic Disruption of the ATP-binding Cassette Transporter BCRP1/ABCG2 Impairs Cardiac Repair After Myocardial ...
... but is instead related to the action of ATP-binding cassette (ABC) transporters that act as ATP-dependent efflux pumps for ... The role of ATP-binding cassette (ABC) transporters in the efflux of the insecticide, temephos, was assessed in the larvae of ... Evaluation of the role of ATP-binding cassette transporters as a defence mechanism against temephos in populations of Aedes ... Multidrug resistance ABC transporters. FEBS letters 555: 102-105. [ Links ] Dermauw W, Van Leeuwen T 2014. The ABC gene family ...
ATP-binding cassette transporter A7 enhances phagocytosis of apoptotic cells and associated ERK signaling in macrophages. ... ATP-binding cassette transporter A7 enhances phagocytosis of apoptotic cells and associated ERK signaling in macrophages ...
... Nat Genet. 1999 Aug;22(4):352-5. ... in which we found the gene encoding human ATP cassette-binding transporter 1 (ABC1). We also found a change in ABC1 expression ... Our findings indicate that defects in ABC1, encoding a member of the ABC transporter superfamily, are the cause of TD. ... ATP Binding Cassette Transporter 1 * ATP-Binding Cassette Transporters / genetics* * Amino Acid Sequence ...
... transporter superfamily. Upon the binding of its periplasmic binding protein, MalE, the ATPase activity of MalFGK2 can be ... Maltose transporter MalFGK2 is a type-I importer in the ATP-binding cassette (ABC) ... Maltose transporter MalFGK2 is a type-I importer in the ATP-binding cassette (ABC) transporter superfamily. Upon the binding of ... Maltose-binding protein effectively stabilizes the partially closed conformation of the ATP-binding cassette transporter MalFGK ...
In different organisms a conserved superfamily proteins known as ATP binding cassette (ABC) participate in various biological ... In different organisms a conserved superfamily proteins known as ATP binding cassette (ABC) participate in various biological ... Shao, R., Shen, Y., Zhou, W., Fang, J., and Zheng, B. (2013). Recent advances for plant ATP-binding cassette transporters. J. ... Genome-Wide Identification and Expression Profiling of ATP-Binding Cassette (ABC) Transporter Gene Family in Pineapple (Ananas ...
... transporters. ABC proteins transport various molecules across extra- and intracellular membranes. This ABCA1 is widely used and ... This membrane-associated protein is a member of the superfamily of ATP-binding cassette (ABC) ...
  • ABC transporters often consist of multiple subunits, one or two of which are transmembrane proteins and one or two of which are membrane-associated ATPases. (wikipedia.org)
  • ABC transporters are considered to be with the ABC superfamily based on the sequence and organization of their ATP-binding cassette (ABC) domains, even though the integral membrane proteins may have evolved independently several times, and thus comprise different protein families. (wikipedia.org)
  • The third subgroup of ABC proteins do not function as transporters, but are rather involved in translation and DNA repair processes. (wikipedia.org)
  • Pathogens use siderophores, such as Enterobactin, to scavenge iron that is in complex with high-affinity iron-binding proteins or erythrocytes. (wikipedia.org)
  • One of the families of genes that have evolved to carry out this function is that which encodes the ATP-binding cassette proteins. (nih.gov)
  • These glutamate and arginine residues are conserved among binding proteins and ABC transporters mediating iron and B 12 uptake, suggesting that they may have a role in docking and the transmission of conformational changes. (pnas.org)
  • ATP binding cassette (ABC) transporters are a ubiquitous family of importer and exporter proteins that invariably consist of two membrane-spanning domains, which form a translocation pathway, and two cytoplasmic ABC domains, which power the transport reaction through binding and hydrolysis of ATP ( 1 ). (pnas.org)
  • Although most eukaryotic ABC transporters export hydrophobic molecules from the cytoplasm ( 2 ), the majority of bacterial ABC transporters import essential nutrients that are delivered to them by specific binding proteins ( 1 , 3 , 4 ). (pnas.org)
  • These proteins bind their substrates selectively and with high affinity, which is thought to ensure the specificity of the transport reaction ( 3 ). (pnas.org)
  • The structures of many different binding proteins have been solved, revealing a common architecture: two domains, each consisting of a central β-sheet and surrounding α-helices, with the substrate binding site located in a cleft between them ( 7 ). (pnas.org)
  • These results provide general insights into the interaction of binding proteins with their cognate ABC transporters. (pnas.org)
  • Active drug efflux transporters of the ATP binding cassette (ABC)-containing family of proteins have a major impact on the pharmacological behavior of most of the drugs in use today. (nih.gov)
  • Borst P, Evers R, Kool M and Wijnholds J (2000) A family of drug transporters: the multidrug resistance‐associated proteins. (els.net)
  • The nucleotide-binding domains and subunits share considerable sequence homology across the entire family ( 45 ) and assume a similar three-dimensional fold that consists of a core nucleotide-binding subdomain that is common to other ATPases and an alpha-helical subdomain that is specific to ABC proteins ( 50 ). (asm.org)
  • This minireview summarizes recent work using vanadate to stabilize the transition state for ATP hydrolysis that has provided new insight into the mechanism of action of this family of proteins. (asm.org)
  • A fuller understanding of how these binding proteins function in transport was realized following our recent observation in the maltose transport system that the periplasmic maltose binding protein (MBP) becomes tightly bound to the membrane transporter (MalFGK 2 , a complex of MalF, MalG, and two MalK proteins) in the presumed catalytic transition state for ATP hydrolysis ( 22 ). (asm.org)
  • These proteins contain two homologous halves, each with six membrane spanning segments and a predicted ATP nucleotide binding domain. (nih.gov)
  • The ATP-binding cassette (ABC) transporter superfamily is one of the largest groups of proteins and plays a non-negligible role in phase III of the detoxification process, which is highly involved in the response of insects to environmental stress (plant secondary metabolites and insecticides). (mdpi.com)
  • Membrane transport proteins belonging to the ATP-binding cassette (ABC) family of transport proteins play a central role in the defence of organisms against toxic compounds, including anticancer drugs. (tudelft.nl)
  • Focus was set on the ATP-binding cassette (ABC) transport proteins expressed in the canalicular membrane of human hepatocytes. (diva-portal.org)
  • ATP-binding cassette (ABC) transporters are a large family of proteins implicated in physiological cellular functions. (eurekaselect.com)
  • In different organisms a conserved superfamily proteins known as ATP binding cassette (ABC) participate in various biological processes. (frontiersin.org)
  • ATP binding cassette (ABC) transporters are ATP-dependent membrane proteins predominantly expressed in excretory organs, such as the liver, intestine, blood-brain barrier, blood-testes barrier, placenta, and kidney. (aspetjournals.org)
  • ABCG proteins are composed of an N-terminal nucleotide-binding domain followed by a membrane domain with six putative transmembrane helices (TMHs). (aspetjournals.org)
  • The observation that many ABCG proteins can interact with steroids raises interesting questions about the nature of protein-steroid interactions in these transporters. (aspetjournals.org)
  • Transcript levels of ten-drug efflux proteins of the ATP-binding cassette (ABC) transporter family [MDR1, MDR3, ABCB5, MRP1-6, and breast cancer resistance protein (BCRP)], lung resistance-related protein (LRP), and CYP3A4 were determined using quantitative polymerase chain reaction in jejunal biopsies from 13 healthy human subjects and in Caco-2 cells. (aspetjournals.org)
  • Multidrug resistance ATP-binding cassette (ABC) efflux transporters are one example of cryptic membrane proteins. (ucsd.edu)
  • Here, we review advances in our understanding of ABC transporters in sea urchin embryos, and methods developed to spatially and temporally map these proteins. (ucsd.edu)
  • The ATP-binding cassette (ABC) transporter family (ATP-dependent pumps) consist of ubiquitously membrane-bound proteins, present in all prokaryotes, as well as plants, fungi, yeast and animals. (axonmedchem.com)
  • Finally, we found that all TKIs were able to compete labeling of ABCB1 and ABCG2 by the photo-cross-linkable prazosin analog [ 125 I]iodoarylazidoprazosin, suggesting interaction at the prazosin-binding site of both proteins. (aspetjournals.org)
  • Contributions of both ATP-binding cassette transporter and Cyp51A proteins are essential for azole resistance in Aspergillus fumigatus. (aspergillus.org.uk)
  • A mechanism commonly observed in pathogenic yeast such as Candida albicans involves gain-of-function mutations in transcriptional regulatory proteins that induce expression of ATP-binding cassette (ABC) transporter encoding genes. (aspergillus.org.uk)
  • The multidomain integral membrane proteins of detoxification enzymes such as glutathione S-transferases (GSTs), carboxylesterases (CarEs) and cytochrome P450 monooxygenases ABC transporters utilize the energy produced by ATP hydrolysis to (P450s) (Ranson et al. (deepdyve.com)
  • Cosilencing of both proteins did not further reduce HDL binding, internalization, or transport. (ahajournals.org)
  • ATP-binding cassette (ABC) transporters represent a group of evolutionarily highly conserved cellular transmembrane proteins that mediate the ATP-dependent translocation of substrates across membranes. (columbia.edu)
  • The comparison between these contrasting states (outward v. inward transport of the same substrate) will allow us to dissect whether sterol transport across the plasma membrane is defined by the molecule (i.e. the ABC transporter) or by microenvironment (i.e. the status of other proteins and lipids) in which it resides. (columbia.edu)
  • The studies presented in this thesis provide evidence that direction of transport is not a static inherent property of the transporter, but rather that it is mutable and influenced by surrounding sterols and proteins. (columbia.edu)
  • Many ABC proteins include two of each type of domain and are so-called full transporters. (biomedcentral.com)
  • The ATP-binding cassette transporter ABCC6 belongs to a large family of membrane proteins (ABC transporters) that are a highly conserved and present in all organisms from bacteria to man [ 1 , 2 ]. (biomedcentral.com)
  • Import of substrates into peroxisomes for β-oxidation is mediated by ATP binding cassette (ABC) transporter proteins of subfamily D, which includes the human adrenoleukodystropy protein (ALDP) defective in X-linked adrenoleukodystrophy (X-ALD). (semanticscholar.org)
  • ATP-binding cassette (ABC) proteins comprise one of the largest gene families, and are found across prokaryotic and eukaryotic domains [ 24 ]. (biomedcentral.com)
  • Most of these proteins function as transmembrane transporters, which actively move a myriad of molecules across cellular membranes [ 25 ]. (biomedcentral.com)
  • ABC transporter proteins have a two-domain structure: a highly conserved nucleotide-binding domain (NBD) and a variable transmembrane domain (TMD) [ 26 ]. (biomedcentral.com)
  • ABC transporter proteins require two NBDs and two TMDs for functionality. (biomedcentral.com)
  • Proteins are classified as ABC transporters based on the sequence and organization of their ATP-binding domain(s), also known as nucleotide-binding folds (NBFs). (chemeurope.com)
  • All ABC proteins also contain either one or two ATP-binding domain(s) , (nucleotide-binding folds (NBFs)) and are located on the cytoplasm side of the membrane. (chemeurope.com)
  • Unlike the Walker A and Walker B motifs, which are found in other proteins which hydrolyze ATP, the signature motif is unique to ABC transporters. (chemeurope.com)
  • The ATP-binding cassette (ABC) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. (biomedcentral.com)
  • ATP-binding cassette (ABC) transporters constitute one of the largest protein superfamilies of integral membrane proteins. (biomedcentral.com)
  • ATP binding cassette (ABC) transport proteins are particularly involved in drug deposition, as they are located at membranes of many uptake and excretory organs and at protective barriers, where they export endogenous and xenobiotic compounds, including pharmaceuticals. (ru.nl)
  • The effect of the anti-malarials on the ATP-dependent uptake of radio-labelled substrates was measured in membrane vesicles isolated from HEK293 cells overexpressing the ABC transport proteins. (ru.nl)
  • P-glycoprotein (ABCB1), multidrug resistance protein-1 (ABCC1) and breast cancer resistance protein (ABCG2) belong to the ATP-binding cassette (ABC) superfamily of proteins that play an important physiological role in protection of the body from toxic xenobiotics and endogenous metabolites. (cdrjournal.com)
  • All ABC proteins share a highly conserved region in the ATP binding domain, including the Walker A and Walker B sequences, the ABC signature motif, the H loop and the Q loop [ 8 ] . (cdrjournal.com)
  • The subfamily ABCE belongs to the non-transporters, whose proteins play a role in the assembly of the pre-initiation complex and RNAse inhibition [ 11 , 12 ] . (cdrjournal.com)
  • The ATP-binding cassette (ABC) proteins are one of the largest superfamily of proteins found in both prokaryotes and higher eukaryotes. (frontiersin.org)
  • A large number of ABC proteins are multidrug transporters and function as efflux pumps. (frontiersin.org)
  • Most but not all uptake systems also have an extracytoplasmic receptor, a solute binding protein. (wikipedia.org)
  • Bacterial binding protein-dependent ATP binding cassette (ABC) transporters facilitate uptake of essential nutrients. (pnas.org)
  • The crystal structure of Escherichia coli BtuF, the protein that binds vitamin B 12 and delivers it to the periplasmic surface of the ABC transporter BtuCD, reveals a bi-lobed fold resembling that of the ferrichrome binding protein FhuD. (pnas.org)
  • The association of a substrate-loaded binding protein with its cognate transporter has been shown to stimulate ATP hydrolysis by the cytoplasmic ABC domains ( 5 ). (pnas.org)
  • The binding protein remains docked to the cognate transporter until one or both of the hydrolysis products are released, as shown by experiments that used vanadate to trap an intermediate close to the transition state ( 6 ). (pnas.org)
  • This finding suggested that the binding protein, associated with the transporter during substrate translocation, may prevent the escape of substrate into the periplasmic space. (pnas.org)
  • Recently, the crystal structure of the binding protein-dependent ABC transporter, BtuCD, which facilitates import of vitamin B 12 into Escherichia coli , was determined at 3.2-Å resolution ( 8 ). (pnas.org)
  • We have now solved the crystal structure of E. coli BtuF, the cognate periplasmic binding protein for BtuCD ( 9 , 10 ) at 2.0-Å resolution. (pnas.org)
  • BtuF with bound vitamin B 12 was crystallized at 4°C by vapor diffusion in hanging drops containing 2 μl of protein and 2 μl of reservoir solution [30% PEG 400/0.1 M sodium acetate (pH 4.6)/0.1 M cadmium chloride/Hampton Crystal Screen 2, #12]. (pnas.org)
  • 2007) Asymmetry in the Structure of the ABC Transporter Binding Protein Complex BtuCD‐BtuF. (els.net)
  • We have assigned the 1 H, 15 N, 13 C backbone resonances of the second periplasmic loop P2 of the MalF subunit of the maltose ATP binding cassette transporter of Escherichia coli / Salmonella which is important for the recognition of the maltose binding protein MalE. (springer.com)
  • Boos W, Lucht JM (1996) Periplasmic binding protein-dependant ABC transporters. (springer.com)
  • Shilton BH, Shuman HA, Mowbray SL (1996) Crystal structures and solution conformations of a dominantnegative mutant of E . coli maltose-binding protein. (springer.com)
  • Studies of periplasmic binding protein-dependent transporters date to the 1960s when it was realized that the transport of certain solutes was inhibited following an osmotic shock that released the contents of the periplasm ( 8 , 80 ). (asm.org)
  • The ATP-binding cassette (ABC) superfamily ( 52 ) was defined in 1986 when homology was detected between a binding protein-dependent transporter and a multidrug efflux pump cloned from human cancer cells ( 21 , 42 ). (asm.org)
  • In the periplasmic binding protein-dependent transport systems, the soluble binding protein is the first component to interact with the substrate to be transported, acting as a high-affinity receptor for the substrate in the periplasm ( 93 ) (see Fig. 1 ). (asm.org)
  • Interaction of the ligand-bound binding protein with the transporter stimulates the ATPase activity of the transporter and initiates transport ( 27 , 88 ). (asm.org)
  • In the transition state conformation, the affinity of the binding protein for maltose is reduced ( 22 ) and the sugar is presumably transferred to the transporter and ultimately into the cell (Fig. 1 ). (asm.org)
  • Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily. (nih.gov)
  • STE6, the yeast a-factor transporter, is a member of the ATP binding cassette protein superfamily, which also includes the mammalian multidrug resistance protein and the cystic fibrosis gene product. (nih.gov)
  • To examine further the functional contribution of each half of the STE6 protein, we severed the STE6 coding sequence and expressed the two halves of the transporter as separate polypeptides. (nih.gov)
  • p>This subsection of the 'Function' section describes a region in the protein which binds nucleotide phosphates. (uniprot.org)
  • Upon the binding of its periplasmic binding protein, MalE, the ATPase activity of MalFGK 2 can be greatly enhanced. (rsc.org)
  • In addition, the ABC transporters, P-glycoprotein (P-gp/ ABCB1 ) and breast cancer resistance protein (BCRP/ ABCG2 ), are highly expressed in a population of primitive stem cells: the side population (SP). (aspetjournals.org)
  • The human breast cancer resistance protein is an ATP-binding cassette (ABC) multidrug transporter that affects the bioavailability of chemotherapeutic drugs and can confer drug resistance on cancer cells. (aspetjournals.org)
  • The molecular bases of protein-steroid interactions in ABC transporters are unknown. (aspetjournals.org)
  • ASKO versus control adipose tissue had decreased PPARγ (peroxisome proliferator-activated receptor γ) and CCAAT/enhancer-binding protein expression, nuclear SREBP1 (sterol regulatory element-binding protein 1) protein, lipogenesis, and triglyceride accretion but similar Akt activation after acute insulin stimulation. (ovid.com)
  • We describe the development of expression systems for simultaneous expression of the ATP-binding protein DrrA and the membrane integral protein DrrB which together behave as a functional doxorubicin efflux pump. (portlandpress.com)
  • Further, the inhibition of the ABC transporters breast cancer resistance protein (BCRP/ABCG2) and multidrug-resistance associated protein 2 (MRP2/ABCC2) was experimentally investigated using structurally diverse datasets that were representative of orally administered drugs. (avhandlingar.se)
  • The ATP-binding cassette transporter ABCG2 (also named breast cancer resistance protein, BCRP) functions as a drug efflux transporter and is expressed at high levels in the human small intestine. (diva-portal.org)
  • Sequence analysis revealed significant amino acid homology to the primary structures of PMR1 (protein encoded by the PMR1 gene) and ABC transporters of Saccharomyces cerevisiae (PDR5 and SNQ2), Schizosaccharomyces pombe (HBA2), Candida albicans (CDR1), and Aspergillus nidulans (AtrA and AtrB). (asm.org)
  • ATP binding cassette transporter G1 (ABCG1) mediates the cholesterol transport from cells to high-density lipoprotein (HDL), but the role of apolipoprotein A-I (apoA-I), the main protein constituent of HDL, in this process is not clear. (ebscohost.com)
  • The p-glycoprotein (PGP, P-gp) and the breast cancer resistance protein (BCRP) both are members of this large family of transporters. (axonmedchem.com)
  • The ATP binding cassette B/multidrug-resistance/P-glycoprotein (ABCB/MDR/PGP) subfamily is a member of the ABC protein family. (springer.com)
  • Among the postulated mechanisms of resistance is the overexpression of ATP-binding cassette (ABC) transporters, such as P-glycoprotein (ABCB1) and breast cancer resistance protein (ABCG2), which mediate reduced intracellular drug accumulation. (aspetjournals.org)
  • We and others have found that an ABC transporter protein called Cdr1B (here referred to as AbcG1) is required for wild-type azole resistance in A.fumigatus Here we test the genetic relationship between the TR34 L98H allele of cyp51A and an abcG1 null mutation. (aspergillus.org.uk)
  • A xenobiotic transporter protein encoded by ABCG2 on chromosome 4q22, belonging to the ATP-binding cassette (ABC) superfamily, White subfamily. (thefreedictionary.com)
  • The report provides comprehensive information on the ATP Binding Cassette Sub Family A Member 1 (ATP Binding Cassette Transporter 1 or ABC 1 or ATP Binding Cassette 1 or Cholesterol Efflux Regulatory Protein or ABCA1), targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. (reportsnreports.com)
  • Additionally, the report provides an overview of key players involved in ATP Binding Cassette Sub Family A Member 1 (ATP Binding Cassette Transporter 1 or ABC 1 or ATP Binding Cassette 1 or Cholesterol Efflux Regulatory Protein or ABCA1) targeted therapeutics development and features dormant and discontinued projects. (reportsnreports.com)
  • The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. (mybiosource.com)
  • The three complexes of ABCB10/ATP analogs reported here showed varying degrees of opening of the transport substrate binding site, indicating that in this conformation there is some flexibility between the two halves of the protein. (cam.ac.uk)
  • In between the TMs is a ligand binding-domain, which is on the extracellular side of the protein for importers and on the cytoplasmic side for exporters. (chemeurope.com)
  • These folds form the "cassettes" which the protein family is named after. (chemeurope.com)
  • Here we show that the solute binding protein (BlG16BP) associated with an ATP binding cassette (ABC) transporter from the probiotic Bifidobacterium animalis subsp. (lu.se)
  • The prokaryotic ABC importers exhibit an additional functional unit, a substrate-binding protein (SBP). (biomedcentral.com)
  • Berkower, C & Michaelis, S 1991, ' Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily ', EMBO Journal , vol. 10, no. 12, pp. 3777-3785. (elsevier.com)
  • We recently cloned a full-length cDNA of the rat ATP-binding cassette transporter 2 (ABC2, or ABCA2) protein, a member of the ABC1 (or ABCA) subfamily (-ABC1/ABCA1 is a causal gene for Tangier disease) and found it to be strongly expressed in the rat brain. (elsevier.com)
  • This superfamily is subdivided into seven distinct groups (ABCA through ABCG) and currently at least 15 ABC transporters have been implicated to confer resistance to clinically active drugs, notably P-glycoprotein (P-gp, ABCB1), multidrug resistance protein-1 (MRP1, ABCC1) and breast cancer resistance protein (BCRP, ABCG2) [ 9 ] . (cdrjournal.com)
  • ABCF subfamily members also encode non-transporters, they are involved in ribosome assembly and protein translation [ 13 , 14 ] . (cdrjournal.com)
  • ATP-binding cassette super-family G member 2 is a protein that in humans is encoded by the ABCG2 gene . (wikipedia.org)
  • The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters . (wikipedia.org)
  • Alternatively referred to as the breast cancer resistance protein ( BCRP ), this protein functions as a xenobiotic transporter which may play a role in multi-drug resistance to chemotherapeutic agents including mitoxantrone and camptothecin analogues. (wikipedia.org)
  • TAP2 (Transporter 2, ATP Binding Cassette Subfamily B Member) is a Protein Coding gene. (genecards.org)
  • Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. (genecards.org)
  • ATP-binding cassette transporters (ABC transporters) are members of a transport system superfamily that is one of the largest and is possibly one of the oldest families with representatives in all extant phyla from prokaryotes to humans. (wikipedia.org)
  • Dean M, Rzhetsky A and Allikmets R (2001) The human ATP‐binding cassette (ABC) transporter superfamily. (els.net)
  • Dean M and Annilo T (2005) Evolution of the ATP‐binding cassette (ABC) transporter superfamily in vertebrates. (els.net)
  • To assess the importance of the two halves of STE6, and to examine the functional significance of residues conserved among members of the ATP binding cassette superfamily, we introduced mutations into the nucleotide binding domains of STE6. (nih.gov)
  • Our findings indicate that defects in ABC1, encoding a member of the ABC transporter superfamily, are the cause of TD. (nih.gov)
  • Maltose transporter MalFGK 2 is a type-I importer in the ATP-binding cassette (ABC) transporter superfamily. (rsc.org)
  • Here, we describe ABCG26, a member of the ATP-binding cassette (ABC) transporter superfamily, which is required for pollen exine formation in Arabidopsis ( Arabidopsis thaliana ). (plantphysiol.org)
  • 2003). Metabolic re- ABC transporters belong to a large superfamily responsible for sistance and target resistance are the two major mechanisms under- the transport of specific molecules across lipid membranes, in add- lying insecticide resistance. (deepdyve.com)
  • In mammalian cells, sterol efflux is a key component of the homeostatic equation and is mediated by members of the ATP binding cassette (ABC) transporter superfamily. (columbia.edu)
  • Many members of the superfamily of ABC transporters have been linked with resistance to multiple drugs or toxins. (biomedcentral.com)
  • A total of 165 ABC transporter genes, constituting a highly expanded superfamily relative to its size in other eukaryotes, were identified in the macronuclear genome of the ciliate Tetrahymena thermophila . (biomedcentral.com)
  • A comprehensive microarray analysis revealed divergent expression patterns among the members of the ABC transporter superfamily during different states of physiology and development. (biomedcentral.com)
  • Gene duplications have resulted in an extensive expansion of the superfamily of ABC transporters in the Tetrahymena genome, making it the largest example of its kind reported in any organism to date. (biomedcentral.com)
  • Clearly, the ABC transporter superfamily in unicellular free-living species requires additional study. (biomedcentral.com)
  • ABCA12 belongs to a large superfamily of the ATP-binding cassette (ABC) transporters, which aid in the transport of various biomolecules across the limiting membrane. (nagoya-u.ac.jp)
  • Specifically, several members of the ATP-binding cassette (ABC) superfamily mediate the efflux of multiple chemotherapeutic drugs and so contribute to MDR. (cdrjournal.com)
  • ATP binding cassette (ABC) transporters are a diverse superfamily of energy-dependent membrane translocases. (mblwhoilibrary.org)
  • ATP-binding cassette (ABC) superfamily members have a key role as nutrient importers and exporters in bacteria. (frontiersin.org)
  • Our study has analyzed the entire complement of ABC superfamily transporters to assess whether these play a major role in drug resistance mechanisms of C. auris . (frontiersin.org)
  • The adenosine triphosphate (ATP)‐binding cassette (ABC) genes encode transporters that are expressed on the cell membrane or organelle membranes and are involved in the export of substances out of the cell or the movement of compounds inside the cell. (els.net)
  • Dean M and Allikmets R (1995) Evolution of ATP‐binding cassette transporter genes. (els.net)
  • The genes encoding ATP-binding cassette (ABC) transporters occupy 2.5% of the genome of Mycobacterium tuberculosis . (portlandpress.com)
  • In the most recent study, atrA and atrB genes encoding ABC transporters were cloned from a filamentous fungus, A. nidulans , and it was demonstrated that an atrB transgene rendered Saccharomyces cerevisiae resistant to azole fungicide ( 6 ). (asm.org)
  • The expression of multidrug efflux transporter genes was therefore examined in the azole-susceptible and azole-resistant yeast isolates. (asm.org)
  • For this purpose, C. glabrata genes conferring resistance to azole antifungals were cloned in a Saccharomyces cerevisiae strain in which the ATP binding cassette (ABC) transporter gene PDR5 was deleted. (asm.org)
  • Three different genes were recovered, and among them, only C. glabrata CDR1 ( CgCDR1 ), a gene similar to the Candida albicans ABC transporter CDR genes, was upregulated by a factor of 5 to 8 in the azole-resistant isolates. (asm.org)
  • Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and. (deepdyve.com)
  • ATP-binding cassette transporter genes also contain the signature C motif, located just up- (ABC) transporters have been identified as important detoxification stream of the Walker B site (Dean et al. (deepdyve.com)
  • Multiple independent duplications and subsequent divergence contributed to the formation of different families of ABC transporter genes. (biomedcentral.com)
  • Characterization and expression profiling of ATP-binding cassette transporter genes in the diamondback moth, Plutella xylostella (L. (biomedcentral.com)
  • In advance of an assembled and annotated D. v. virgifera genome, we developed transcriptomic resources to use in identifying candidate genes likely to be involved in the evolution of resistance, starting with members of the ATP-binding cassette (ABC) transporter family. (biomedcentral.com)
  • Phylogenetic analysis placed the deduced amino-acid sequences of the Dvv ABC transporters into eight subfamilies (A to H). To supplement our sequence data with functional analysis, we identified orthologs of Tribolium castaneum ABC genes which had previously been shown to exhibit overt RNA interference (RNAi) phenotypes. (biomedcentral.com)
  • TAP2 is one of two MHC genes whose products form a transporter molecule involved in endogenous antigen processing. (allelebiotech.com)
  • We have identified 73 ABC transporter genes in the genome of T. castaneum , which group into eight subfamilies (ABCA-H). This coleopteran ABC family is significantly larger than those reported for insects in other taxonomic groups. (biomedcentral.com)
  • Affected individuals typically have complete genetic deficiency - homozygous loss-of-function (LoF) variants - in the ATP-binding cassette transporter G5 ( ABCG5 ) or G8 ( ABCG8 ) genes, and have substantially elevated plasma sitosterol and low-density lipoprotein cholesterol (LDL-C) levels. (rxivist.org)
  • Homologs of other ABC transporter genes, such as CDR4 , CDR6 , and SNQ2 , also displayed raised expression in a sub-set of clinical isolates. (frontiersin.org)
  • The ATPase subunits utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to energize the translocation of various substrates across membranes, either for uptake or for export of the substrate. (wikipedia.org)
  • ABC transporters utilize the energy of ATP binding and hydrolysis to transport various substrates across cellular membranes. (wikipedia.org)
  • In the closed conformation, MBP binds MalFGK 2 to initiate transport and hydrolysis. (asm.org)
  • B) In the transition state for ATP hydrolysis, MBP becomes tightly bound to MalFGK 2 , and internal sugar-binding sites are exposed to each other. (asm.org)
  • In eukaryotes, ABC transporters that utilize the energy of ATP hydrolysis to expel cellular substrates into the environment are responsible for most of the efflux from cells. (biomedcentral.com)
  • Particular areas of controversy include the roles of ATP binding, ATP hydrolysis and the subsequent dissociation of the ADP and Pi. (chemeurope.com)
  • By coupling ATP binding and hydrolysis these transporters are able to extrude substrates out of cells or into cellular compartments like cytoplasmic vesicles and lysosomes [ 8 ] . (cdrjournal.com)
  • CHO cells transfected with ABCA2 did not have a higher rate of endogenous ATP hydrolysis when compared to the mock-transfected cells. (elsevier.com)
  • Introduction Recently, the ATP-binding cassette transporter BCRP1/ABCG2 has been shown to regulate the function and survival of side population cells, which have been identified in various organs including heart and have stem cell properties. (ahajournals.org)
  • Here, we identify a steroid-binding element in the membrane domain of ABCG2 with a similarity to steroid hormone/nuclear receptors. (aspetjournals.org)
  • The element facilitates steroid hormone binding and mediates modulation of ABCG2 activity. (aspetjournals.org)
  • In this article, we describe the identification of a functional steroid-binding element in ABCG2 R482G . (aspetjournals.org)
  • The half-transporter ABCG2 and the widely studied P-glycoprotein (P-gp) are two ABC transporters that, when overexpressed, are capable of extruding a variety of structurally unrelated chemotherapy agents from cells. (aspetjournals.org)
  • We also demonstrated, for the first time, that [ 125 I]iodoarylazidoprazosin, a photoaffinity analog of the substrate prazosin, labels multiple variants of ABCG2 specifically and that this labeling, although competed by some ABCG2 substrates, is unaffected by cyclosporin A. These labeling data also suggest the presence of multiple drug binding sites in ABCG2. (aspetjournals.org)
  • Together, our results suggest that cyclosporin A is neither a substrate nor an inhibitor of the human ABCG2 transporter, under the conditions and concentrations examined. (aspetjournals.org)
  • Cytotoxicity assays in stably transduced K562-ABCG2 and K562-ABCB1 cells confirmed that the TKIs were also substrates for the two transporters. (aspetjournals.org)
  • ABCG2, also termed BCRP / MXR / ABCP, is a half ATP-binding cassette (ABC) transporter expressed on plasma membranes. (eurekaselect.com)
  • ABCG2 consists of a nucleotide-binding domain (NBD) at the amino terminus and a transmembrane domain (TMD) at the carboxyl terminus and it is postulated to form a homodimer to perform its biological functions. (eurekaselect.com)
  • Cells isolated based on the ALDEFLUOR™ assay were compared to cells isolated based on ATP binding cassette transporter G2 (ABCG2) activity using the side population assay. (biomedcentral.com)
  • ABCG2, containing only 1 MSD and 1 NBD) and non-transporters, which possess two NBDs, without the MSDs [ 10 ] . (cdrjournal.com)
  • Methods We have here conducted a comprehensive set of in vitro and in vivo experiments to determine to what extent two dominant efflux transporters in the BBB, P-gp (ABCB1) and BCRP (ABCG2), exhibit affinity towards AZD1775 and PD0166285 and restrict their brain penetration. (qxmd.com)
  • abstract = "ATP-binding cassette (ABC) transporter A4 is a member of the ABC transporter subfamily A which has been reported to be exclusively expressed in the retina. (elsevier.com)
  • The membrane-spanning region of the ABC transporter protects hydrophilic substrates from the lipids of the membrane bilayer thus providing a pathway across the cell membrane. (wikipedia.org)
  • A typical ABC transporter has four domains or subunits, two of which are hydrophobic and are predicted to span the membrane multiple times in an alpha-helical conformation and two of which bind nucleotide and are exposed to the cytoplasm. (asm.org)
  • C) Maltose is transported, and MBP is released after reexposure of the membrane-binding site to the cytoplasm. (asm.org)
  • ATP-binding cassette transporter A1 (ABCA1) has been found to mediate the transfer of cellular cholesterol across the plasma membrane to apolipoprotein A‑I (apoA‑I), and is essential for the synthesis of high‑density lipoprotein. (spandidos-publications.com)
  • To summarize, the present work has led to an increased knowledge of the molecular features of importance for ABC transporter inhibition and passive membrane permeability. (avhandlingar.se)
  • While it is well appreciated that membrane transport is critical for development, the specific roles of many transporters have remained cryptic, in part because of their abundance and the diversity of their substrates. (ucsd.edu)
  • Nonetheless, the challenges underlying the identification of embryonic membrane transporter function are essential to tackle for several reasons. (ucsd.edu)
  • The first is simply that the functions of many membrane transporters are incompletely understood in any system. (ucsd.edu)
  • This is especially relevant given that the expression of membrane transporters in diseases such as cancer can itself result from recapitulation of developmental pathways, including the epithelial-to-mesenchymal transformation pathways. (ucsd.edu)
  • Besides, the nucleotide binding domain (NBD or ATP binding cassette), these transporters also contain trans-membrane domains (TMDs), each of which comprises several hydrophobic α-helices. (axonmedchem.com)
  • This mutant is defective in PLEIOTROPIC DRUG RESISTANCE8 (PDR8)/PENETRATION3/ABCG36, a plasma membrane-localized ATP binding cassette transporter that has established roles in pathogen responses and cadmium transport. (plantcell.org)
  • We propose that co-localization and complex formation of sterol esterification enzymes and ABC transporters in DRMs reflects a novel mechanism that directs membrane sterols to the esterification reaction. (columbia.edu)
  • The data provided here offers further insight as to how ABC transporters move cholesterol from the membrane and therefore may provide a platform for innovative strategies to combat atherosclerosis. (columbia.edu)
  • ABCB10 is one of the three ATP-binding cassette (ABC) transporters found in the inner membrane of mitochondria. (cam.ac.uk)
  • The crystal structure of apo-ABCB10 shows a classic exporter fold ABC transporter structure, in an open-inwards conformation, ready to bind the substrate or nucleotide from the inner mitochondrial matrix or membrane. (cam.ac.uk)
  • Within eukaryotes, ABC-transporters mainly transport molecules to the outside of the plasma membrane or into membrane-bound organelles such as the endoplasmic reticulum, mitochondria , etc. (chemeurope.com)
  • Structurally, each ABC transporter is composed of at least one hydrophobic membrane-spanning domain (MSD) consisting of 6 α-helices and 1 hydrophilic nucleotide binding domain (NBD). (cdrjournal.com)
  • Here we show that both an ATP-binding cassette (ABC) transporter(s) and an H+-antiporter(s) are involved in the uptake of cyanidin 3-O-glucoside (C3G) by Arabidopsis vacuolar membrane-enriched vesicles. (ecu.edu)
  • The transporter has been shown to play protective roles in blocking absorption at the apical membrane of the intestine, and at the blood-testis barrier , [8] the blood-brain barrier , [8] and the membranes of hematopoietic progenitor and other stem cells . (wikipedia.org)
  • Here we examine these processes in ABCB10 (ABC-me), a mitochondrial inner membrane erythroid transporter involved in heme biosynthesis. (mblwhoilibrary.org)
  • 1999) Genotype/phenotype analysis of a photoreceptor‐specific ATP‐binding cassette transporter gene, ABCR, in Stargardt disease. (els.net)
  • The ATP-binding cassette transporters: structure, function, and gene family comparison between rice and Arabidopsis. (uniprot.org)
  • The identification of the defective Tangier disease gene as ABCA1 has contributed substantially to the understanding of its role as a key transporter of cellular cholesterol and phospholipids across cell membranes to acceptor molecules such as apolipoprotein (apo) A-I. Interestingly, ABCA1 is widely expressed throughout many animal tissues, where it may have multiple and diverse functions ( 5 ). (diabetesjournals.org)
  • We report here refining of our previous linkage of the TD gene to a 1-cM region between markers D9S271 and D9S1866 on chromosome 9q31, in which we found the gene encoding human ATP cassette-binding transporter 1 (ABC1). (nih.gov)
  • Genome-Wide Identification and Expression Profiling of ATP-Binding Cassette (ABC) Transporter Gene Family in Pineapple (Ananas comosus (L.) Merr. (frontiersin.org)
  • The mammalian ATP-binding cassette transporters A1 and A7 (ABCA1 and -A7) show sequence similarity to CED-7, a Caenorhabditis elegans gene that mediates the clearance of apoptotic cells. (rupress.org)
  • A PMR1 ( Penicillium multidrug resistance) gene encoding an ATP-binding cassette (ABC) transporter (P-glycoprotein) was cloned from a genomic DNA library of a DMI-resistant strain (LC2) of Penicillium digitatum by heterologous hybridization with a DNA fragment containing an ABC-encoding region from Botrytis cinerea . (asm.org)
  • AIM To determine whether mutations in the Stargardt's disease gene, ATP binding cassette transporter retina (ABCR) affect the occurrence of age related macular degeneration (AMD) in Japanese non-familial patients. (bmj.com)
  • Recently, Allikmets et al reported an association of mutations in the Stargardt's disease gene, ATP binding cassette transporter retina (ABCR), with AMD. (bmj.com)
  • We recently showed that the ATP-binding-cassette (ABC) transporter gene CgCDR1 was upregulated in C. glabrata clinical isolates resistant to azole antifungal agents (D. Sanglard, F. Ischer, D. Calabrese, P. A. Majcherczyk, and J. Bille, Antimicrob. (asm.org)
  • In the HFAR strain derived from DSY1041, a distinct ABC transporter gene similar to CgCDR1 , called CgCDR2 , was upregulated. (asm.org)
  • When CgCDR1 obtained from an azole-susceptible isolate was reintroduced with the help of a centromeric vector in DSY1041, azole resistance was restored and thus suggested that a trans -acting mutation(s) could be made responsible for the increased expression of this ABC transporter gene in the azole-resistant strain. (asm.org)
  • This study demonstrates for the first time the determinant role of an ABC transporter gene in the acquisition of resistance to azole antifungals by C. glabrata clinical isolates. (asm.org)
  • The ATP-binding cassette transporter ABCC6 gene is located on chromosome 16 between its two pseudogenes ( ABCC6P1 and ABCC6P2 ). (biomedcentral.com)
  • Several studies have been reported that ATP Binding Cassette Transporter A1 (ABCA1) gene polymorphisms in known as one of the genetic risk factor for T2DM. (alliedacademies.org)
  • Recently, many studies have been focused on the association between the ATP-binding cassette transporter A1 (ABCA1) gene R219K polymorphism and ischemic stroke (IS). (cdc.gov)
  • Gene Ontology (GO) annotations related to this gene include transporter activity and ATPase activity, coupled to transmembrane movement of substances . (genecards.org)
  • Unlike other tyrosine kinase inhibitors, sorafenib and sunitinib do not appear to rely on active transport to enter the cell nor are they high-affinity substrates for ABC efflux transporters. (aacrjournals.org)
  • Unlike other tyrosine kinase inhibitors registered for the treatment of cancer, such as those that inhibit the BCR-Abl oncogene or epidermal growth factor receptor, the multikinase inhibitors sorafenib and sunitinib do not appear to rely on active transport to enter the cell nor are they high-affinity substrates for ATP-binding cassette (ABC) efflux transporters. (aacrjournals.org)
  • Studies have shown that tyrosine kinase inhibitors are substrates for and/or inhibit the function of various ATP-binding cassette (ABC) transporters, and these interactions may play an important role in modulating systemic pharmacokinetics of drugs, tissue and brain distribution, and cellular accumulation and resistance ( 6 - 16 ). (aacrjournals.org)
  • Our experiments support the hypothesis that all three TKIs are substrates of ABC transporters and that, at higher concentrations, TKIs overcome transporter function. (aspetjournals.org)
  • The involvement hydrolyze ATP, as well as two transmembrane domains (TMDs), of ABC transporters in xenobiotic resistance for arthropods was his- which are involved in the translocation of their respective substrates torically not well documented, but an increasing number of studies (Rees et al. (deepdyve.com)
  • In this conformation, the substrate binding is lower affinity, and so the substrates can be released. (chemeurope.com)
  • They are ubiquitously found in all kingdoms of life, where they typically function in the ATP-dependent transport of various substrates across biological membranes. (biomedcentral.com)
  • Since then, numerous ABC transporters have been shown to be involved in transport of various substrates in a wide range of eukaryotic organisms. (biomedcentral.com)
  • The eukaryotic ABC transporters are promiscuous exporters and can extrude a variety of substrates including metals, drugs, xenobiotics, lipids, and other cellular metabolites ( Prasad and Goffeau, 2012 ). (frontiersin.org)
  • Bioassays were conducted using mosquito populations that were either susceptible or resistant to temephos by exposure to insecticide alone or in combination with sublethal doses of the ABC transporter inhibitor, verapamil (30, 35 and 40 μM). (scielo.br)
  • Doxorubicin uptake in Escherichia coli or Mycobacterium smegmatis expressing DrrAB was inhibited by reserpine, an inhibitor of ABC transporters. (portlandpress.com)
  • Larvae were treated with acaricide alone and acaricide in combination with a sub-lethal dose of the ABC transporter inhibitor cyclosporine A . The LC50 doses and 95% confidence intervals (CIs) estimated by mortality data using probit analysis were 67.930 p.p.m. (95% CI 53.780-90.861) for fipronil and 3741 p.p.m. (95% CI 2857-4647) for ivermectin . (bvsalud.org)
  • It has long been hypothesized that inhibition of ABC drug efflux transporters will increase drug accumulation and thereby overcome MDR, but until now no approved inhibitor of these transporters is available in the clinic. (cdrjournal.com)
  • Decreased affinity for efflux transporters increases brain penetrance and molecular targeting of a PI3K/mTOR inhibitor in a mouse model of glioblastoma. (qxmd.com)
  • Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. (genecards.org)
  • This review focuses on the role of ABC transporters in tissue defense and regeneration, with specific attention to P-gp and BCRP in organ regeneration and repair. (aspetjournals.org)
  • The majority of the inhibitors affected all three major ABC transporters BCRP, MRP2 and P-glycoprotein (P gp/ABCB1), and these multi-specific inhibitors were found to be enriched in highly lipophilic weak bases. (avhandlingar.se)
  • Using RNA interference, we investigated the impact of SR-BI, ABCA1, and ABCG1 on binding, internalization, and transcytosis of HDL by ECs. (ahajournals.org)
  • HDL binding was reduced by 50% and 30% after silencing of SR-BI and ABCG1, respectively, but not at all after diminishing ABCA1 expression. (ahajournals.org)
  • We hypothesized that spherical HDL crosses the endothelium similar to lipid-free apoA-I and therefore investigated binding, uptake, and transport of HDL by cultured aortic ECs and the involvement of the ATP-binding cassette transporters ABCA1 and ABCG1, as well as the HDL receptor scavenger receptor (SR)-BI. (ahajournals.org)
  • Specifically, we expressed murine ABCG1 (mABCG1) in yeast and assessed how changes in the intracellular sterol environment affect movement of sterols by this transporter. (columbia.edu)
  • ATP-binding cassette transporters, ABCA1 and ABCG1, are cell surface transporters that mediate the reverse cholesterol transport (RCT), a process that delivers cholesterol from the peripheral tissue back to the liver for disposal [ 2 ]. (alliedacademies.org)
  • ABCG1: Transporter involved in macrophage lipid homeostasis. (mybiosource.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human ATP Binding Cassette Transporter C11 (ABCC11) in Tissue homogenates, cell lysates and other biological fluids. (wildpalm.net)
  • Levels of IFN-γ secretion by Vγ9/Vδ2 T cells were profoundly increased by pAg loading, or by binding of the pan-BTN3A specific agonist antibody CD277 20.1, in HeLa-M compared to HeLa-L cells. (cf.ac.uk)
  • Potential role of ATP-binding cassette transporters against acaricides in the brown dog tick Rhipicephalus sanguineus sensu lato. (bvsalud.org)
  • Many ABC transporters may be classified as half transporters or full transporters . (chemeurope.com)
  • Half transporters consist of only one TM and one NBF and must combine with another half transporter to gain functionability. (chemeurope.com)
  • Half transporters can thus form homodimers if two identical ABC transporters join, and heterodimers if two unlike ABC transporters join. (chemeurope.com)
  • Either they are full-transporters combining all four domains (2 TMDs and 2 NBDs) in a single polypeptide, or they are half-transporters, consisting of only two domains (1 TMD and 1 NBD), which can be arranged either as TMD-NBD or NBD-TMD. (biomedcentral.com)
  • The half-transporters need to form homo- or heterodimers to form a functional pump. (biomedcentral.com)
  • ABCB1 and ABCC1, consisting of 2 MSDs and 2 NBDs), half transporters (e.g. (cdrjournal.com)
  • Full transporters consist of the typical two TMs and NBFs. (chemeurope.com)
  • Depending on the clustering of the MSD and the NBD in the transporters, these are grouped into full transporters (e.g. (cdrjournal.com)
  • Structures of ABCB10, a human ATP-binding cassette transporter in apo- and nucleotide-bound states. (cam.ac.uk)
  • To compare side-by-side the uptake of sorafenib and sunitinib in vitro by human uptake solute carriers of the SLC22A and SLCO families, the transport by and inhibition of efflux ATP-binding cassette (ABC) transporters, and the role of ABCB1 in the plasma pharmacokinetics and brain penetration of these agents. (aacrjournals.org)
  • In vivo pharmacokinetic studies were done in knockout mice lacking Abcb1-type transporters. (aacrjournals.org)
  • Sorafenib and sunitinib showed concentration-dependent (1 and 10 μmol/L), low to moderate affinity for ABCB1 but were not affected by the other ABC transporters. (aacrjournals.org)
  • The NBD provides energy by hydrolyzing ATP, and the TMDs determines the substrate specificity ( Schneider and Hunke, 1998 ). (frontiersin.org)
  • Although most organisms utilize these ABC transporters during embryonic development, many of these transporters have broad substrate specificity, and their developmental functions remain incompletely understood. (ucsd.edu)
  • These structures suggest that the observed plasticity, together with a portal between two helices in the transmembrane region of ABCB10, assist transport substrate entry into the substrate binding cavity. (cam.ac.uk)
  • The NBD binds and hydrolyzes ATP to provide the energy required for translocating a substrate across cell membranes, while the TMD forms a channel through which the substrate is transported [ 27 ]. (biomedcentral.com)
  • These transmembrane domains provide the specificity for the substrate, and prevent unwanted molecules from using the transporter. (chemeurope.com)
  • These studies identified residues important for substrate recognition and transport activity in AtABCC2, and suggest that C3G and GSH bind closely, mutually enhancing each other's binding. (ecu.edu)
  • However, competitive binding of a pseudo-substrate, 8-azido-[α- 32 P]-ATP, was demonstrated. (elsevier.com)
  • Putative steroid-binding element in ABCG subfamily. (aspetjournals.org)
  • However, none of these putative ABC transporters has been characterized so far. (portlandpress.com)
  • The phenotypes conferred by pen3 result from the loss of function of PLEIOTROPIC DRUG RESISTANCE8 (PDR8), a highly expressed putative ATP binding cassette transporter. (plantcell.org)
  • In this study, 65 putative D. v. virgifera ABC ( Dvv ABC) transporters were identified within a combined transcriptome assembly generated from embryonic, larval, adult male, and adult female RNA-sequence libraries. (biomedcentral.com)
  • We performed ligand docking studies to a homology model of AtABCC2 and probed the putative binding sites of C3G and GSH through site-directed mutagenesis and functional studies. (ecu.edu)
  • MBP activates the ATPase activity of MalK by bringing the two MalK subunits into close proximity, completing the nucleotide-binding site(s) at the MalK-MalK interface with residues donated from the opposing subunit. (asm.org)
  • Home Research Outputs An atp binding cassette transporter mediates the uptake of α. (lu.se)
  • By comparison, B. ovatus mono-cultures grew very efficiently on this trisaccharide These findings suggest that the ABC-mediated uptake of raffinose provides an important competitive advantage, particularly against dominant Bacteroides that lack glycan-specific ABC-transporters. (lu.se)
  • Mice with genetic deletion of the cholesterol efflux transporter, ATP-binding cassette (ABC) G1, have pulmonary lipidosis and chronic pulmonary inflammation. (nih.gov)
  • Although ATP-binding cassette transporter A1 (ABCA1) is well known for its role in cholesterol efflux and HDL formation, it is expressed in various tissues, where it may have different functions. (diabetesjournals.org)
  • The ATP-binding cassette transporter-1 (ABCA1) mediates cholesterol efflux and genotypic variation in ABCA1 and may impact reverse cholesterol transport and influence cardiovascular disease (CVD) risk. (biomedsearch.com)
  • ABC transporters have a modular structure, consist- searching the transcriptome dataset, which may be involved in the ing of two nucleotide-binding domains (NBDs), which bind and insecticide detoxification displayed by these moths. (deepdyve.com)
  • The ABC transporter core unit consists of four domains, two NBDs and two TMDs. (axonmedchem.com)
  • The transporter associated with antigen processing (TAP) is an ATP-binding cassette (ABC) transporter essential to cellular immunity against viral infection. (rcsb.org)
  • Ambudkar SV and Gottesman MM (1998) ABC transporters: biochemical, cellular, and molecular aspects. (els.net)
  • Inhibitors of these transporters are thus potentially useful tools to reverse this transporter-mediated cellular resistance to anticancer drugs and, eventually, to enhance the effectiveness of the treatment of patients with drug-resistant cancer. (tudelft.nl)
  • The aim of this study was to evaluate the role of the cellular cholesterol exporter, Abca1 (ATP-binding cassette transporter A1), on adipose tissue function during diet-induced obesity. (ovid.com)
  • Based on these characteristics, sorafenib and sunitinib may be less susceptible to transporter-mediated alterations in plasma pharmacokinetics, tissue distribution, cellular accumulation, and drug resistance. (aacrjournals.org)
  • Hundreds of ABC transporters have been characterized from both prokaryotes and eukaryotes. (wikipedia.org)
  • ABC transporters that function as exporters are found in eukaryotes and prokaryotes, whereas ABC importers seem to be restricted to prokaryotes. (biomedcentral.com)
  • ABC transporters are present in all living species, in prokaryotes they function as drug importers or exporters, in eukaryotes solely as drugs exporters [ 15 ] . (cdrjournal.com)
  • ABC transporters (P-glycoproteins) are known to be responsible for multidrug resistance in prokaryotic and eukaryotic organisms ( 2 , 15 , 16 , 31 , 35 ). (asm.org)
  • Eukaryotic ABC transporters usually consist of two types of domains, a transmembrane domain (TMD) and a nucleotide-binding domain (NBD). (biomedcentral.com)
  • There are two schemes by which the domains of eukaryotic ABC transporters are organized. (biomedcentral.com)
  • Sequence analyses of eukaryotic ABC transporters revealed that they can be divided into eight subfamilies (ABCA-H). Subfamily ABCH, which is most closely related to subfamily ABCG, was identified first in the Drosophila melanogaster genome. (biomedcentral.com)
  • The first eukaryotic ABC transporter identified was the P-glycoprotein on the cell surface of cancer cells [ 6 ], which acts as a multidrug resistance (MDR) efflux transporter that prevents the accumulation of chemotherapeutic drugs. (biomedcentral.com)
  • B 12 is bound in the "base-on" conformation in a deep cleft formed at the interface between the two lobes of BtuF. (pnas.org)
  • A) MBP binds maltose, undergoing a change from an open conformation to a closed conformation, generating a high-affinity sugar-binding site. (asm.org)
  • Unexpectedly, however, ABCB10 adopts an open-inwards conformation when complexed with nonhydrolysable ATP analogs, in contrast to other transporter structures which adopt an open-outwards conformation in complex with ATP. (cam.ac.uk)
  • These structures indicate that ABC transporters may exist in an open-inwards conformation when nucleotide is bound. (cam.ac.uk)
  • Many other xenotoxins, (pre-)carcinogens and endogenous compounds are also influenced by the ABC transporters, with corresponding consequences for the well-being of the individual. (nih.gov)
  • The TKIs inhibited rhodamine 123 and Hoechst 33342 efflux mediated by endogenous expression of the transporters in murine and human hematopoietic stem cells with potency order nilotinib ≫ imatinib ≫ dasatinib. (aspetjournals.org)
  • These include nearly 1,000 transporters that translocate nutrients, signaling molecules, and xenobiotics across membranes. (ucsd.edu)
  • Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. (genecards.org)
  • This domain architecture is clearly established in the recent structure of MsbA ( 20 ), an ABC transporter that mediates the export of lipid and lipid A in E. coli ( 32 , 133 ). (asm.org)
  • 8 Recently, we have shown that ECs bind, internalize, and transport lipid-free apoA-I in a specific manner. (ahajournals.org)
  • In the plasma, the majority of apoA-I is lipid-bound and assembled in spherical HDL. (ahajournals.org)
  • Some of the ABC transporters we studied in closer detail to examine their role in lipid, ecdysteroid and eye pigment transport. (biomedcentral.com)
  • ATP -binding cassette transporter A1 (ABCA1) promotes cholesterol and phospholipid efflux from cells to lipid-poor apolipoprotein A-I ( apoA-I ), and plays a key role in the initial steps of the whole process of reverse cholesterol transport (RCT). (bvsalud.org)
  • ABCA12 is a keratinocyte lipid transporter associated with lipid transport in lamellar granules and loss of ABCA12 function leads to a defective lipid barrier in the stratum corneum and disturbed keratinocyte differentiation. (nagoya-u.ac.jp)
  • It is known that the ATP-binding cassette transporter A1 (ABCA1) plays a major role in cholesterol homeostasis and high density lipoprotein (HDL) metabolism. (ebscohost.com)
  • Intrinsic acyl-CoA thioesterase activity of a peroxisomal ATP binding cassette transporter is required for transport and metabolism of fatty acids. (semanticscholar.org)
  • Further, as alluded to above, the actual function of those transporters in disease can be analogous to their developmental roles, such as controlling cell motility. (ucsd.edu)
  • Apolipoprotein A-I but not high-density lipoproteins are internalised by RAW macrophages: roles of ATP-binding cassette transporter A1 and scavenger receptor BI. (ebscohost.com)
  • Moreover, we have identified 65 members of the ABC transporter family and provided the first insights into the developmental and physiological roles of ABC transporters in this pest species. (biomedcentral.com)
  • Background: ABCA2 is a member of the ATP binding cassette transporter family with functional roles in cholesterol homeostasis and drug resistance. (elsevier.com)
  • Several ATP-binding cassette (ABC) transporters can confer multidrug resistance to cancer cells by functioning as energy-dependent efflux pumps. (aspetjournals.org)
  • We aim to provide an overview of properties of the mammalian ABC transporters known to mediate significant transport of clinically relevant drugs. (nih.gov)
  • In the present study, in Nilaparvata lugens , we identified 32 ABC transporters, which are grouped into eight subfamilies (ABCA-H) based on phylogenetic analysis. (mdpi.com)
  • Most ABC transporter subfamilies had highly conservative amino acid sequence domains: the nucleotide-binding domain (NBDs) and the transmembrane domains (TMDs) including five or six helices ( Higgins and Linton, 2004 ). (frontiersin.org)
  • The ABCE and ABCF subfamilies lack of TMDs domain and do not function as transporter. (frontiersin.org)
  • Also, a large number of ABC transporters are mainly known as expressed sequence tags and have been assigned to different subfamilies according to their similarity to known ABC transporters. (aspetjournals.org)
  • More recently ABC-transporters have been shown to exist within the placenta, indicating they could play a protective role for the developing fetus against xenobiotics . (chemeurope.com)
  • The capacity of ABC transporters to efflux a broad spectrum of xenobiotics represents one of the major mechanisms of clinical multidrug resistance in pathogenic fungi including Candida species. (frontiersin.org)
  • It is known that when an ATP molecule binds to each cassette of an ABC-transporter, it induces a conformational change in which the NBFs interact more closely. (chemeurope.com)
  • To date, mammalian ABC transporters are exclusively associated with efflux of cholesterol. (columbia.edu)
  • The last two decades have seen notable growth of knowledge concerning the involvement of ABC transporters in resistance to chemotherapy. (unamur.be)
  • Recent achievements regarding tumor cell biology are expected to provide a rationale for developing novel inhibitors that target ABC transporters implicated in drug resistance. (eurekaselect.com)
  • The resistant phenotype could be reversed by verapamil and reserpine, two potent inhibitors of ABC transporters. (portlandpress.com)
  • Previously unknown ABC transporter inhibitors were identified and predictive computational models were developed for the different drug transport mechanisms. (avhandlingar.se)
  • Conclusion The brain penetration of these Wee1 inhibitors is severely limited by ABC transporters, which may compromise their clinical efficacy against intracranial neoplasms such as DIPG and GBM. (qxmd.com)
  • Daus ML, Landmesser H, Schlosser A, Müller P, Herrmann A, Schneider E (2006) ATP induces conformational changes of periplasmic loop regions of the maltose ATP-binding cassette transporter. (springer.com)
  • The transcellular transport of apoA-I is temperature-dependent, involves the ATP-binding cassette transporter (ABC)A1, and generates a lipidated particle. (ahajournals.org)
  • In Saccharomyces cerevisiae, we have demonstrated that the opposite (i.e inward) transport of sterol in yeast is also dependent on two ABC transporters (Aus1p and Pdr11p). (columbia.edu)
  • This prompts the question what dictates directionality of sterol transport by ABC transporters. (columbia.edu)
  • This is the first example of an ABC transporter mediating bi-directional transport. (columbia.edu)
  • These data suggest that direction of transport is not a static property of the transporter but rather can adapt in response to changes in the intracellular microenvironment. (columbia.edu)
  • Here, the role of ABC transporters in the transport of fipronil and ivermectin acaricides in the tick Rhipicephalus sanguineus ( Ixodida Ixodidae ) was investigated. (bvsalud.org)
  • Pharmacological properties affected by ABC transporters include the oral bioavailability, hepatobiliary, direct intestinal, and urinary excretion of drugs and drug-metabolites and -conjugates. (nih.gov)
  • Moreover, the penetration of drugs into a range of important pharmacological sanctuaries, such as brain, testis, and fetus, and the penetration into specific cell- and tissue compartments can be extensively limited by ABC transporters. (nih.gov)
  • Beyond this, these transporters determine the toxicity profile of many drugs, and confer multidrug resistance (MDR) in cancer cells associated with a poor treatment outcome of cancer patients. (cdrjournal.com)
  • ABC transporters are also involved in multiple drug resistance, and this is how some of them were first identified. (wikipedia.org)
  • Kolaczkowski M and Goffeau A (1997) Active efflux by multidrug transporters as one of the strategies to evade chemotherapy and novel practical implications of yeast pleiotropic drug resistance. (els.net)
  • ATP -binding cassette (ABC) transporters have been shown to be involved in pesticide detoxification in arthropod vectors and are thought to contribute to the development of drug resistance . (bvsalud.org)
  • How to overcome ATP-binding cassette drug efflux transporter-mediated drug resistance? (cdrjournal.com)
  • A better definition of the molecular events clarifying the regulation of transporter levels including regulation by microRNAs may contribute to provide new molecular tools to target such a family of transporters. (eurekaselect.com)
  • Molecular analysis of well-known preclinical systems as well as of cancer stem cell models supports the notion that ABC transporters represent amenable targets for modulation of the efficacy of antitumor agents endowed with different molecular features. (eurekaselect.com)
  • To clarify the role of MalE binding and to gain deeper understanding of the molecular mechanisms of MalFGK 2 , we calculated the free energy surfaces (FESs) related to the lateral motion in the presence and absence of MalE using atomistic metadynamics simulations. (rsc.org)
  • Toward understanding the mechanism of action of the yeast multidrug resistance transporter Pdr5p: a molecular modeling study. (ebi.ac.uk)
  • Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. (genecards.org)
  • MsbA is a homodimer, and the intracellular loops between transmembrane-spanning helices of each monomer unit constitute a novel intracellular alpha-helical domain that physically separates the nucleotide-binding domain from the transmembrane domain. (asm.org)
  • Transmembrane drug export mediated by the ATP-binding cassette (ABC) transporter P-glycoprotein contributes to clinical resistance to antineoplastics. (aacrjournals.org)
  • Oldham ML, Khare D, Quiocho FA, Davidson AL, Chen J (2007) Crystal structure of a catalytic intermediate of the maltose transporter. (springer.com)