AT Rich Sequence
Base Sequence
Molecular Sequence Data
GC Rich Sequence
DNA
Clustered adenine/thymine stretches are essential for function of a fission yeast replication origin. (1/203)
We have determined functional elements required for autonomous replication of the Schizosaccharomyces pombe ars2004 that acts as an intrinsic chromosomal replication origin. Internal deletion analysis of a 940-bp fragment (ars2004M) showed three regions, I to III, to be required for autonomously replicating sequence (ARS) activity. Eight-base-pair substitutions in the 40-bp region I, composed of arrays of adenines on a DNA strand, resulted in a great reduction of ARS activity. Substitutions of region I with synthetic sequences showed that no specific sequence but rather repeats of three or more consecutive adenines or thymines, without interruption by guanine or cytosine, are required for the ARS activity. The 65-bp region III contains 11 repeats of the AAAAT sequence, while the 165-bp region II has short adenine or thymine stretches and a guanine- and cytosine-rich region which enhances ARS activity. All three regions in ars2004M can be replaced with 40-bp poly(dA/dT) fragments without reduction of ARS activity. Although spacer regions in the ars2004M enhance ARS activity, all could be deleted when an 40-bp poly(dA/dT) fragment was added in place of region I. Our results suggest that the origin activity of fission yeast replicators depends on the number of adenine/thymine stretches, the extent of their clustering, and presence of certain replication-enhancing elements. (+info)The centromeric sister chromatid cohesion site directs Mcd1p binding to adjacent sequences. (2/203)
Cohesion between sister chromatids occurs along the length of chromosomes, where it plays essential roles in chromosome segregation. We show here that the centromere, a cis-acting cohesion factor, directs the binding of Mcd1p, a cohesin subunit, to at least 2 kb regions flanking centromeres in a sequence-independent manner. The centromere is essential for the maintenance as well as the establishment of this cohesin domain. The efficiency of Mcd1p binding within the cohesin domain is independent of the primary nucleotide sequence of the centromere-flanking DNA but correlates with high A + T DNA content. Thus, the function of centromeres in the cohesion of centromere-proximal regions may be analogous to that of enhancers, nucleating cohesin complex binding over an extended chromosomal domain of A + T-rich DNA. (+info)Nuclear protein binding at the beta-myosin heavy chain A/T-rich element is enriched following increased skeletal muscle activity. (3/203)
In adult mouse skeletal muscle, beta-myosin heavy chain (betaMyHC) gene expression is primarily restricted to slow-type I fibers but can be induced in fast-type II fibers by mechanical overload (MOV). Our previous transgenic analyses have delimited an 89-base pair (bp) MOV-responsive region (-293 to -205), and shown that mutation of the MCAT and C-rich elements within this region did not abolish betaMyHC transgene induction by MOV. In this study we describe an A/T-rich element (betaA/T-rich; -269 5'-GGAGATATTTTT-3' -258) located within this 89-bp region that, only under MOV conditions, revealed enriched binding as characterized by electrophoretic mobility shift assays and dimethyl sulfate and diethyl pyrocarbonate interference footprinting. Direct, competition, and supershift electrophoretic mobility shift assays revealed highly enriched specific binding activity at the betaA/T-rich element that was antigenically distinct from GATA-4, MEF2A-D, SRF, and Oct-1, nuclear proteins that were previously shown to bind A/T-rich elements. In vitro translated GATA-4, MEF2C, SRF, and Oct-1 bound to consensus GATA, MEF2, SRE, and Oct-1 elements, respectively, but not to the betaA/T-rich element. Two-dimensional UV cross-linking of the bromodeoxyuridine-substituted betaA/T-rich element with mechanically overloaded plantaris (MOV-P) nuclear extract detected two proteins (44 and 48 kDa). Our results indicate that the betaA/T-rich element may function in vivo as a betaMyHC MOV-inducible element during hypertrophy of adult skeletal muscle by binding two distinct proteins identified only in MOV-P nuclear extract. (+info)Sequence evolution in bacterial endosymbionts having extreme base compositions. (4/203)
A major limitation on ability to reconstruct bacterial evolution is the lack of dated ancestors that might be used to evaluate and calibrate molecular clocks. Vertically transmitted symbionts that have cospeciated with animal hosts offer a firm basis for calibrating sequence evolution in bacteria, since fossils of the hosts can be used to date divergence events. Sequences for a functionally diverse set of genes have been obtained for bacterial endosymbionts (Buchnera) from two pairs of aphid host species, each pair diverging 50-70 MYA. Using these dates and estimated numbers of Buchnera generations per year, we calculated rates of base substitution for neutral and selected sites of protein-coding genes and overall rates for rRNA genes. Buchnera shows homogeneity among loci with regard to synonymous rate. The Buchnera synonymous rate is about twice that for low-codon-bias genes of Escherichia coli-Salmonella typhimurium on an absolute timescale, and fourfold higher on a generational timescale. Nonsynonymous substitutions show a greater rate disparity in favor of Buchnera, a result consistent with a genomewide decrease in selection efficiency in Buchnera. Ratios of synonymous to nonsynonymous substitutions differ for the two pairs of Buchnera, indicating that selection efficiency varies among lineages. Like numerous other intracellular bacteria, such as Rickettsia and Wolbachia, Buchnera has accumulated amino acids with codons rich in A or T. Phylogenetic reconstruction of amino acid replacements indicates that replacements yielding increased A + T predominated early in the evolution of Buchnera, with the trend slowing or stopping during the last 50 Myr. This suggests that base composition in Buchnera has approached a limit enforced by selective constraint acting on protein function. (+info)Cell type-specific autoregulation of the Caudal-related homeobox gene Cdx-2/3. (5/203)
The caudal-related homeobox gene Cdx-2/3 is a critical "master" control gene in embryogenesis. Mice heterozygous for a null mutation in Cdx-2/3 exhibit multiple malfunctions including tail abnormalities, stunted growth, a homeotic shift in vertebrae, and the development of multiple intestinal adenomatous polyps, indicating that Cdx-2/3 is haplo-insufficient. In vitro studies have identified more than a half-dozen downstream target genes expressed in pancreatic and intestinal cells for this transcription factor. We have examined the transcriptional properties of the mouse Cdx-2/3 promoter. This promoter could be autoregulated in pancreatic and intestinal cells that express endogenous Cdx-2/3. In contrast, Cdx-2/3 transfection represses the Cdx-2/3 promoter in fibroblasts, which do not express endogenous Cdx-2/3. Since Cdx-2/3 activates proglucagon gene promoter in both pancreatic and intestinal cells and in fibroblasts, we suggest that some, yet to be identified, cell type-specific components are required for activating selected target gene promoters of Cdx-2/3, including the Cdx-2/3 promoter itself. Cdx-2/3 binds to the TATA box and another AT-rich motif, designated as DBS, within an evolutionarily conserved proximal element of the Cdx-2/3 promoter. The DBS motif is critical for the autoregulation, whereas the TATA box may act as an attenuating element for the autoregulatory loop. Finally, overexpression of Cdx-2/3 in a pancreatic cell line activated the expression of the endogenous Cdx-2/3. Taken together, our results indicate that the dose-dependent phenotype of Cdx-2/3 expression on its downstream targets in vivo could be regulated initially via a transcriptional network involving cell type-specific autoregulation of the Cdx-2/3 promoter. (+info)Host sequences flanking the human T-cell leukemia virus type 1 provirus in vivo. (6/203)
Human pathogenic retroviruses do not have common loci of integration. However, many factors, such as chromatin structure, transcriptional activity, DNA-protein interaction, CpG methylation, and nucleotide composition of the target sequence, may influence integration site selection. These features have been investigated by in vitro integration reactions or by infection of cell lines with recombinant retroviruses. Less is known about target choice for integration in vivo. The present study was conducted in order to assess the characteristics of cellular sequences targeted for human T-cell leukemia virus type 1 (HTLV-1) integration in vivo. Sequencing integration sites from >/=200 proviruses (19 kb of sequence) isolated from 29 infected individuals revealed that HTLV-1 integration is not random at the level of the nucleotide sequence. The virus was found to integrate in A/T-rich regions with a weak consensus sequence at positions within and without of the hexameric repeat generated during integration. These features were not associated with a preference for integration near active regions or repeat elements of the host chromosomes. Most or all of the regions of the genome appear to be accessible to HTLV-1 integration. As with integration in vitro, integration specificity in vivo seems to be determined by local features rather than by the accessibility of specific regions. (+info)Start sites of bidirectional DNA synthesis at the human lamin B2 origin. (7/203)
The initiation sites of bidirectional synthesis at the DNA replication origin located at the 3' end of the human lamin B2 gene were investigated. RNA-primed nascent DNA molecules were subjected to second-strand synthesis with appropriate primers, amplified by ligation-mediated polymerase chain reaction, and size fractionated. Evidence for precise start sites was obtained. Exploration of close to 1 kilobase, coupled to inhibition of Okazaki fragment synthesis, demonstrates that the leading strands initiate at precise nucleotides on either helix, overlapping by three base pairs, within the area bound to a protein complex possibly analogous to the prereplicative complex of yeast. (+info)AT-rich palindromes mediate the constitutional t(11;22) translocation. (8/203)
The constitutional t(11;22) translocation is the only known recurrent non-Robertsonian translocation in humans. Offspring are susceptible to der(22) syndrome, a severe congenital anomaly disorder caused by 3&rcolon;1 meiotic nondisjunction events. We previously localized the t(11;22) translocation breakpoint to a region on 22q11 within a low-copy repeat termed "LCR22" and within an AT-rich repeat on 11q23. The LCR22s are implicated in mediating different rearrangements on 22q11, leading to velocardiofacial syndrome/DiGeorge syndrome and cat-eye syndrome by homologous recombination mechanisms. The LCR22s contain AT-rich repetitive sequences, suggesting that such repeats may mediate the t(11;22) translocation. To determine the molecular basis of the translocation, we cloned and sequenced the t(11;22) breakpoint in the derivative 11 and 22 chromosomes in 13 unrelated carriers, including two de novo cases and der(22) syndrome offspring. We found that, in all cases examined, the reciprocal exchange occurred between similar AT-rich repeats on both chromosomes 11q23 and 22q11. To understand the mechanism, we examined the sequence of the breakpoint intervals in the derivative chromosomes and compared this with the deduced normal chromosomal sequence. A palindromic AT-rich sequence with a near-perfect hairpin could form, by intrastrand base-pairing, on the parental chromosomes. The sequence of the breakpoint junction in both derivatives indicates that the exchange events occurred at the center of symmetry of the palindromes, and this resulted in small, overlapping staggered deletions in this region among the different carriers. On the basis of previous studies performed in diverse organisms, we hypothesize that double-strand breaks may occur in the center of the palindrome, the tip of the putative hairpin, leading to illegitimate recombination events between similar AT-rich sequences on chromosomes 11 and 22, resulting in deletions and loss of the palindrome, which then could stabilize the DNA structure. (+info)AT-rich sequence is a DNA sequence that is rich in adenine (A) and thymine (T) nucleotides. These sequences are often found in the non-coding regions of DNA, such as the intergenic regions between genes, and are thought to play a role in regulating gene expression. AT-rich sequences can form stable structures, such as hairpins and triplexes, due to the high number of hydrogen bonds that can be formed between A-T base pairs. These structures can affect the accessibility of DNA to transcription factors and other regulatory proteins, and can also affect the stability of the DNA molecule. In the medical field, AT-rich sequences are often studied in the context of diseases such as cancer, where changes in the structure or function of these sequences can lead to abnormal gene expression and contribute to the development and progression of the disease. They are also studied in the context of gene therapy, where they can be used as targets for the delivery of therapeutic genes to specific cells or tissues.
In the medical field, a base sequence refers to the specific order of nucleotides (adenine, thymine, cytosine, and guanine) that make up the genetic material (DNA or RNA) of an organism. The base sequence determines the genetic information encoded within the DNA molecule and ultimately determines the traits and characteristics of an individual. The base sequence can be analyzed using various techniques, such as DNA sequencing, to identify genetic variations or mutations that may be associated with certain diseases or conditions.
In the medical field, "Base Composition" refers to the relative proportions of the four nitrogenous bases (adenine, guanine, cytosine, and thymine) in DNA or RNA. The base composition of a nucleic acid molecule is determined by the number of each base present and the sequence in which they are arranged. The base composition of DNA is typically expressed as the percentage of each base relative to the total number of bases. For example, if a DNA molecule contains 100 bases and 30% of those bases are adenine, the base composition would be 30% A, 20% T, 20% C, and 30% G. The base composition of RNA is similar to that of DNA, but RNA contains the base uracil (U) instead of thymine (T). The base composition of RNA is typically expressed as the percentage of each base relative to the total number of bases, with the exception of uracil, which is often expressed as the percentage of each base relative to the total number of nucleotides (which includes both bases and sugars). The base composition of nucleic acids can provide important information about the genetic material and can be used to identify different types of organisms or to diagnose genetic disorders.
DNA, or deoxyribonucleic acid, is a molecule that carries genetic information in living organisms. It is composed of four types of nitrogen-containing molecules called nucleotides, which are arranged in a specific sequence to form the genetic code. In the medical field, DNA is often studied as a tool for understanding and diagnosing genetic disorders. Genetic disorders are caused by changes in the DNA sequence that can affect the function of genes, leading to a variety of health problems. By analyzing DNA, doctors and researchers can identify specific genetic mutations that may be responsible for a particular disorder, and develop targeted treatments or therapies to address the underlying cause of the condition. DNA is also used in forensic science to identify individuals based on their unique genetic fingerprint. This is because each person's DNA sequence is unique, and can be used to distinguish one individual from another. DNA analysis is also used in criminal investigations to help solve crimes by linking DNA evidence to suspects or victims.
Tornado outbreak sequence of May 2019
Epitranscriptomic sequencing
Exome sequencing
Whole genome sequencing
Patch-sequencing
Tornado outbreak sequence of May 2003
The Simpsons opening sequence
Sequence learning
Sequences 72 & 73
Sylvester's sequence
Sequence analysis in social sciences
Low-discrepancy sequence
Deterministic Barcoding in Tissue for Spatial Omics Sequencing
Tornado outbreak sequence of March 24-28, 2021
List of sequence alignment software
Whole genome bisulfite sequencing
Single-cell DNA template strand sequencing
Sequence (musical form)
Cambridge Reference Sequence
Sonority Sequencing Principle
Limit of a sequence
Moser-de Bruijn sequence
Longest linear sequence
MicroRNA sequencing
Nanopore sequencing
Human Genome Sequencing Center
List of sequenced plastomes
Shotgun sequencing
Main sequence
De novo peptide sequencing
RCSB PDB - 4QU6: Crystal structure of a G-rich RNA sequence binding factor 1 (GRSF1) from Homo sapiens at 1.75 A resolution
Richard Ansett - Sequences - Waffle House Index
Elon Musk, Richard Branson & Jeff Bezos' Best Advice for Ensuring Your Startup Doesn't Fail | Entrepreneur
Combined analysis of microbial metagenomic and metatranscriptomic sequencing data to assess in situ physiological conditions in...
Researchers Fully Sequence the Y Chromosome for the First Time | NIST
Pagosa Unitarian Universalist Fellowship to hear about the Fibonacci number sequence | The Pagosa Springs SUN
OCR01250 - Secret of Mana 'Tidal Sequence' - ReMix Comments/Reviews - OC ReMix Community
Bullseye Brooklyn: Sponsored by Nobody's Right on Target - The Brooklyn Rail
Interleaving Sequences « Python recipes « ActiveState Code
Port Everglades Inlet Microbiome Analysis Using High-Throughput DNA Sequencing
Fringe Opening Sequence - TV Fanatic
Recombinant Anti-CD42b antibody [SP202] (ab227669) | Abcam
Oral/Lecture - DVCON US 2024
eClassical - Oklahoma!, Act 1: No. 17b, Dream Sequence Out of My Dreams
Exome sequencing of family trios from the National Birth Defects Prevention Study: Tapping into a rich resource of genetic and...
Search | MATHCOUNTS Foundation
Updating .msi deployed via task sequence - Configuration Manager 2012 - www.windows-noob.com
Minority and Special Populations Initiative
SATB2 gene: MedlinePlus Genetics
Whole exome sequencing in family trios reveals de novo mutations in PURA as a cause of severe neurodevelopmental delay and...
Bio::Seq::MetaI - Interface for sequence objects with residue-based meta information - metacpan.org
RICK AND MORTY Will Be Recast As Adult Swim Cuts Ties With Co-Creator Justin Roilland
What is B-Raf?
sequence 24,60,210,504,1716,2730,...,... - New Logic/Math Puzzles - BrainDen.com - Brain Teasers
pbr322 sequence - Hollis-Eden Pharmaceuticals Inc.
Kat14 MGI Mouse Gene Detail - MGI:1917264 - lysine acetyltransferase 14
Genome4
- Led by the National Human Genome Research Institute (NHGRI), a team of researchers at the National Institute of Standards and Technology (NIST) and many other organizations used advanced sequencing technologies to read out the full DNA sequence of the Y chromosome - a region of the genome that typically drives male reproductive development. (nist.gov)
- Last year, a team from the Telomere-to-Telomere (T2T) consortium, which is made up of experts from dozens of organizations such as NIST, generated the most complete reference genome at the time by using new sequencing technologies to crack previously indecipherable regions of the genome. (nist.gov)
- Importantly, we need to advance genome sequencing and oxygen production at national level and to build regional capacities for vaccine production. (who.int)
- Complete Genome Sequence of Cyanobacterium Geminocystis sp. (bvsalud.org)
Exome2
- Background De novo mutations are emerging as an important cause of neurocognitive impairment, and whole exome sequencing of case-parent trios is a powerful way of detecting them. (bmj.com)
- Methods The Deciphering Developmental Disorders study is using whole exome sequencing in family trios to investigate children with severe, sporadic, undiagnosed developmental delay. (bmj.com)
Genomes2
- Using them both as guides for sequencing a diverse group of over 1,200 separate genomes, they found that T2T-Y drastically improved the outcomes. (nist.gov)
- COVID-19-compatible symptoms, and symptomatic HCP sequencing demonstrated that SARS-CoV-2 viral genomes were sent home per MDH and CDC guidance. (cdc.gov)
Nucleotide4
- quence typing with nucleotide sequence typing of 3 antigen The clonal complex distribution of 171 isolates collected genes increased the discriminatory index achieved from in New South Wales, Australia ( 8 ), was distinct from the 0.975 to 0.992 among 620 clinical isolates from Oxford- shire, United Kingdom. (cdc.gov)
- ST catenated nucleotide sequences of the MLST loci, which butions of different transmission routes are poorly quanti- indicated that the Australian dataset was 3.6% differenti- fi ed. (cdc.gov)
- Nucleotide sequence-based isolate characterization shire dataset: 575 (98.6% of isolates) fl aA SVR sequences methods such as multilocus sequence typing (MLST) suc- contained 130 distinct SVR alleles, and 567 (97.1%) fl aB cessfully catalog bacterial pathogens and provide a rational, sequences contained 111 SVR alleles. (cdc.gov)
- Examples of this kind of meta data are secondary structures (RNA and protein), protein hydrophobicity assignments, or other alternative alphabets for polypeptides, sequence quality data and nucleotide alignments with translations. (metacpan.org)
Fragment1
- They examined the sample with a combination of cutting-edge technologies - namely high fidelity and nanopore sequencing - that make the DNA fragment puzzle pieces larger and thus easier to assemble. (nist.gov)
Genetic2
- The results of a study published in Nature demonstrate that this advance improves DNA sequencing accuracy for the chromosome, which could help identify certain genetic disorders and potentially uncover the genetic roots of others. (nist.gov)
- DNA sequencing isn't as simple as reading genetic material from a genome's beginning to its end. (nist.gov)
Protein1
- It phosphorylates a specific sequence on protein targets. (news-medical.net)
Data2
- The length of the meta data sequence is not dependent on the amount of the meta information. (metacpan.org)
- We encourage all countries to enhance sequencing capacity and data sharing. (who.int)
Benchmarks1
- The GIAB's mission is to produce test materials, or benchmarks, that can be used to evaluate sequencing technologies or methods. (nist.gov)
Absent1
- The researchers pitted their complete Y chromosome sequence, named T2T-Y, against the most widely used reference genome's Y chromosome parts, which are riddled with stretches of absent code. (nist.gov)
Molecule1
- Conserved region 2 (CR2) is a serine-rich region that functions as a hinge on the molecule. (news-medical.net)
Implementation1
- Meta provides basic, pure perl implementation of sequences with meta information. (metacpan.org)
Study2
- This study aims to use high-throughput sequencing (HTS) and ion chromatography to determine: the compositions of the microbial communities ("microbiomes") present in the port and their changes over a year, understand how water chemistry changes may correlate with changes in the microbial composition, and determine which harmful human and marine pathogens are present in the port. (nova.edu)
- Case 4 was a phenotypically isolated case recruited into an undiagnosed rare disorders sequencing study. (bmj.com)
Method3
- The materials themselves are highly accurate readouts of specific genes that can act as an answer key for checking the results of a particular sequencing method. (nist.gov)
- Prefix sub , like in subseq(), means that the method applies to sub region of the sequence range and takes start and end as arguments. (metacpan.org)
- Prefix named_ in method names allows the used to attach multiple meta strings to one sequence by explicitly naming them. (metacpan.org)
Region1
- Our Region is rich with human resources, knowledge, and research capacities. (who.int)
Code1
- So, researchers and clinicians rely on special software to piece together fragments of sequenced code in the correct order like a puzzle. (nist.gov)
Length1
- Since it is dependent on the length of the sequence, it needs to be manipulated after the sequence. (metacpan.org)
Complete2
- The development of high-throughput DNA sequencing (also commonly referred to as "Next Generation") techniques have allowed for a more complete characterization of marine microbes to be obtained. (nova.edu)
- If the range is not defined, it defaults to the complete sequence. (metacpan.org)
Important1
- Hidden inside this sequence is another important number in mathematics: the golden ratio. (pagosasun.com)
Made2
- Amid some very troubling allegations made against Rick and Morty co-creator Justin Roiland, Adult Swim has decided to continue the animated series without him - which will mean casting a new voice actor! (comicbookmovie.com)
- James Lewis said that Dick Gammage had made "excellent points" at the last meeting, which Mr. Lewis believed were "focused" and "on target" especially with regards to technical issues. (cdc.gov)
Genomic2
- This Funding Opportunity Announcement (FOA), issued by the National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, encourages applications that propose to perform replication, fine-mapping, and sequencing studies of human genomic regions that are putatively associated with phenotypes relevant to the NIAMS mission. (nih.gov)
- Oxford Nanopore's technology can, with very high accuracy, read any length of native DNA, enabling the identification and comprehensive sequence assessment of genomic insertion sites without the risk of missing off target integrations that may negatively impact the therapeutic or the patient. (businesswire.com)
Centers2
- The Large-Scale DNA Sequencing Project continues this tradition by directing multiple population studies and participant samples through two sequencing centers to streamline data analysis and avoid repetition. (nih.gov)
- This limited competition RFA will use the U54 cooperative agreement mechanism to continue the effort of supporting human microbiome sequencing that was begun in FY07 through funds provided to NHGRI- and NIAID-funded large-scale sequencing centers. (nih.gov)
Proteins2
- The amino acid sequences of fifty-five LRR proteins have been published. (nih.gov)
- Epigenetic alterations (or the "epigenome", when considering epigenetics on a genome-wide scale) include modifications to DNA, histone proteins that serve as a genome scaffold, and other factors including noncoding RNAs that collectively regulate the expression of genes without changing DNA sequence. (nih.gov)
Amino1
- Researchers believe that this abnormal amino acid sequence directs the protein into the nucleus of the cell, where it cannot form the strong network of intermediate filaments. (medlineplus.gov)
Alleles1
- 4. Phylogenetic history of hominoid DRB loci and alleles inferred from intron sequences. (nih.gov)
Genes4
- Van Tuinen, M., Sibley, C. & Hedges, S. B. Phylogeny and biogeography of ratite birds inferred from DNA sequences of the mitochondrial ribosomal genes. (nature.com)
- 5. HLA-DRB intron 1 sequences: implications for the evolution of HLA-DRB genes and haplotypes. (nih.gov)
- 9. Evolutionary relationship of HLA-DRB genes inferred from intron sequences. (nih.gov)
- 16. HLA class II "typing": direct sequencing of DRB, DQB, and DQA genes. (nih.gov)
Cohorts1
- Replication, fine-mapping, and sequencing studies are to be conducted in existing cohorts with defined phenotypes. (nih.gov)
Computational2
- How will a computational pipeline for analyzing raw sequencing data be established? (medscape.com)
- How will the tissue preparation, sequencing and computational pipeline for clinical use be analytically validated? (medscape.com)
Spencer2
Reproducibility1
- Sequence Typing of used to confi rm reproducibility. (cdc.gov)
20181
- Last edit at 08/06/2018 09:25AM by rick reynolds. (nih.gov)
Human2
- Large tumor sequencing studies have demonstrated that human cancers of a given histologic type are often heterogeneous with regard to the mutations that drive their invasion and that the mutations present in individual tumors have major influences on the natural history of the tumor, and its responsiveness to therapy. (medscape.com)
- Large tumor-sequencing studies have established that human cancers of a given histologic type are often heterogeneous with regard to the mutations that drive their growth and invasion. (medscape.com)
Practice1
- We are excited to be working with PathoQuest to develop and offer the first Good Manufacturing Practice (GMP) accredited Integration Site Analysis test with nanopore sequencing. (businesswire.com)
Progress1
- Mr. Brepson continued, "This new Integration Site Analysis method, powered by Oxford Nanopore sequencing, will enable our customers to progress their biologics programmes with more certainty than ever before. (businesswire.com)
Studies1
- With this perspective, we will review some new types of clinical studies that are utilizing high-throughput DNA sequencing. (medscape.com)
Methods2
- Methods inherited from interface org.biojava.nbio.core.sequence.template. (biojava.org)
- Additionally, PathoQuest's ISA test is positioned to provide significant benefits (speed, accuracy, confidence in the results) compared to traditional sequencing methods. (businesswire.com)
Lung1
- Using DNA sequencing technique, we narrowed down a short CpG-rich segment (eight specific CpG sites in the CpG island within exon 1) of the TCF21 gene, which was unmethylated in normal lung epithelial cells but predominantly methylated in lung cancer cell lines. (nih.gov)
Gene1
- Differential methylation of a short CpG-rich sequence within exon 1 of TCF21 gene: a promising cancer biomarker assay. (nih.gov)
Study1
- A. The Sequenced Treatment Alternatives to Relieve Depression (STAR*D) Study, funded by the NIH National Institute of Mental Health, is a nationwide public health clinical trial. (nih.gov)
Time1
- How will sequencing be completed within a clinically relevant time frame? (medscape.com)
Results2
- How will the sequencing results be interpreted and related to treatment options? (medscape.com)
- How will sequencing results be collected and stored? (medscape.com)
Explore1
- Click molecule labels to explore molecular sequence information. (nih.gov)
Address1
- Questions to address in using high-throughput sequencing for oncology. (medscape.com)
Short1
- We specifically targeted this short CpG-rich sequence and developed a quantitative methylation-specific PCR assay suitable for high-throughput analysis. (nih.gov)
Field1
- Richard: Well, all infectious diseases have some common threads and I think once you get into the field of studying infectious diseases you can see similarities and sort of lessons that can be learned from all of these. (nih.gov)
Great1
- Richard made great use of the Wolfram Function Repository -a public resource of user-contributed Wolfram Language functions. (wolfram.com)
Applications1
- With over 20 peer reviewed publications on NGS applications in biosafety testing, PathoQuest is a leading expert in the application of Good Manufacturing Practices (GMP)-compliant next generation sequencing (NGS)-based biosafety testing. (businesswire.com)
Institute1
- Richard: I actually came to this institute as a postdoc on a project studying amyotrophic lateral sclerosis and post-polio syndrome. (nih.gov)
Work1
- Tried sequence as in deadeyes but does not work out any one have an illustration or a monkey see monkey do a 5 year old can understand. (modelshipworld.com)
Video1
- Video by Art of Problem Solving's Richard Rusczyk. (mathcounts.org)
Found1
- this arginine-rich region is not found in the normal keratin 10 protein. (medlineplus.gov)