AT-Hook Motifs
Molecular Sequence Data
Amino Acid Motifs
Amino Acid Sequence
Nucleotide Motifs
Flagella
Base Sequence
Binding Sites
Sequence Homology, Amino Acid
Protein Binding
Protein Structure, Tertiary
Sequence Alignment
Characterisation of a cluster of genes encoding Theileria annulata AT hook DNA-binding proteins and evidence for localisation to the host cell nucleus. (1/23)
Infection of bovine leukocytes by the apicomplexan parasite Theileria annulata results in alteration of host cell gene expression and stimulation of host cell proliferation. At present, the parasite-derived factors involved in these processes are unknown. Recently, we described the characterisation of a parasite gene (TashAT2), whose polypeptide product bears AT hook DNA-binding motifs and may be transported from the parasite to the host nucleus. We now describe the isolation of a further two genes (TashAT1 and TashAT3) that are very closely related to TashAT2. All three TashAT genes are located together in a tight cluster, interspersed by two further small open reading frames, all facing head to tail. TashAT2 was shown to be expressed in all T. annulata cell lines examined, whereas TashAT1 and TashAT3 were expressed in the sporozoite stage of the parasite, and also in infected cell lines, where their expression was found to vary between different cell lines. Evidence for transport was provided by antisera raised against TashAT1 and TashAT3 that reacted with the host nucleus of T. annulata-infected cells. Reactivity was particularly strong against the host nuclei of the T. annulata-infected cloned cell line D7B12, which is attenuated for differentiation. A polypeptide in the size range predicted for TashAT3 was preferentially detected in host enriched D7B12 nuclear extracts. DNA-binding analysis demonstrated that fusion proteins containing the AT hook region of either TashAT1 or TashAT2 bound preferentially to AT rich DNA. (+info)Synthesis of signals for de novo DNA methylation in Neurospora crassa. (2/23)
Most 5-methylcytosine in Neurospora crassa occurs in A:T-rich sequences high in TpA dinucleotides, hallmarks of repeat-induced point mutation. To investigate how such sequences induce methylation, we developed a sensitive in vivo system. Tests of various 25- to 100-bp synthetic DNA sequences revealed that both T and A residues were required on a given strand to induce appreciable methylation. Segments composed of (TAAA)(n) or (TTAA)(n) were the most potent signals; 25-mers induced robust methylation at the special test site, and a 75-mer induced methylation elsewhere. G:C base pairs inhibited methylation, and cytosines 5' of ApT dinucleotides were particularly inhibitory. Weak signals could be strengthened by extending their lengths. A:T tracts as short as two were found to cooperate to induce methylation. Distamycin, which, like the AT-hook DNA binding motif found in proteins such as mammalian HMG-I, binds to the minor groove of A:T-rich sequences, suppressed DNA methylation and gene silencing. We also found a correlation between the strength of methylation signals and their binding to an AT-hook protein (HMG-I) and to activities in a Neurospora extract. We propose that de novo DNA methylation in Neurospora cells is triggered by cooperative recognition of the minor groove of multiple short A:T tracts. Similarities between sequences subjected to repeat-induced point mutation in Neurospora crassa and A:T-rich repeated sequences in heterochromatin in other organisms suggest that related mechanisms control silent chromatin in fungi, plants, and animals. (+info)The Stigmatella aurantiaca homolog of Myxococcus xanthus high-mobility-group A-type transcription factor CarD: insights into the functional modules of CarD and their distribution in bacteria. (3/23)
Transcriptional factor CarD is the only reported prokaryotic analog of eukaryotic high-mobility-group A (HMGA) proteins, in that it has contiguous acidic and AT hook DNA-binding segments and multifunctional roles in Myxococcus xanthus carotenogenesis and fruiting body formation. HMGA proteins are small, randomly structured, nonhistone, nuclear architectural factors that remodel DNA and chromatin structure. Here we report on a second AT hook protein, CarD(Sa), that is very similar to CarD and that occurs in the bacterium Stigmatella aurantiaca. CarD(Sa) has a C-terminal HMGA-like domain with three AT hooks and a highly acidic adjacent region with one predicted casein kinase II (CKII) phosphorylation site, compared to the four AT hooks and five CKII sites in CarD. Both proteins have a nearly identical 180-residue N-terminal segment that is absent in HMGA proteins. In vitro, CarD(Sa) exhibits the specific minor-groove binding to appropriately spaced AT-rich DNA that is characteristic of CarD or HMGA proteins, and it is also phosphorylated by CKII. In vivo, CarD(Sa) or a variant without the single CKII phosphorylation site can replace CarD in M. xanthus carotenogenesis and fruiting body formation. These two cellular processes absolutely require that the highly conserved N-terminal domain be present. Thus, three AT hooks are sufficient, the N-terminal domain is essential, and phosphorylation in the acidic region by a CKII-type kinase can be dispensed with for CarD function in M. xanthus carotenogenesis and fruiting body development. Whereas a number of hypothetical proteins homologous to the N-terminal region occur in a diverse array of bacterial species, eukaryotic HMGA-type domains appear to be confined primarily to myxobacteria. (+info)A Theileria annulata DNA binding protein localized to the host cell nucleus alters the phenotype of a bovine macrophage cell line. (4/23)
The apicomplexan parasite Theileria annulata is the only intracellular eukaryote that is known to induce the proliferation of mammalian cells. However, as the parasite undergoes stage differentiation, host cell proliferation is inhibited, and the leukocyte is eventually destroyed. We have isolated a parasite gene (SuAT1) encoding an AT hook DNA binding polypeptide that has a predicted signal peptide, PEST motifs, nuclear localization signals, and domains which indicate interaction with regulatory components of the higher eukaryotic cell cycle. The polypeptide is localized to the nuclei of macroschizont-infected cells and was detected at significant levels in cells that were undergoing parasite stage differentiation. Transfection of an uninfected transformed bovine macrophage cell line, BoMac, demonstrated that SuAT1 can modulate cellular morphology and alter the expression pattern of a cytoskeletal polypeptide in a manner similar to that found during the infection of leukocytes by the parasite. Our findings indicate that Theileria parasite molecules that are transported to the leukocyte nucleus have the potential to modulate the phenotype of infected cells. (+info)The herpesvirus saimiri open reading frame (ORF) 50 (Rta) protein contains an at hook required for binding to the ORF 50 response element in delayed-early promoters. (5/23)
The herpesvirus saimiri open reading frame (ORF) 50 encodes two proteins, which activate transcription directly, following interactions with delayed-early (DE) promoters containing a specific motif. In this report, we demonstrate that ORF 50 contains a DNA binding domain that has homology to an AT hook DNA binding motif. Deletion analysis of this domain reduces ORF 50-mediated transactivation of the DE ORF 6 and ORF 57 promoters by 100 and 90%, respectively. Furthermore, gel retardation experiments demonstrated that the AT hook motif is required for binding the ORF 50 response element in the promoters of DE genes. Single site-directed mutagenesis of the AT hook revealed that mutation of the glycine residue at position 408 to an alanine reduces ORF 50 transactivation of the ORF 57 promoter by 40%. Moreover, the mutation of multiple basic residues in conjunction with the glycine residue within the core element of the AT hook abolishes ORF 50-mediated transactivation. In addition, p50GFPDeltaAT-hook is capable of functioning as a trans-dominant mutant, leading to a reduction in virus production of approximately 50% compared to that for wild-type ORF 50. (+info)A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo. (6/23)
Lens epithelium-derived growth factor p75 (LEDGF/p75) is a DNA-binding, transcriptional co-activator that participates in HIV-1 integration site targeting. Using complementary approaches, we determined the mechanisms of LEDGF/p75 DNA-binding in vitro and chromatin-association in living cells. The binding of highly-purified, recombinant protein was assayed by surface plasmon resonance (SPR) and electrophoretic mobility gel shift. Neither assay revealed evidence for sequence-specific DNA-binding. Residues 146-197 spanning the nuclear localization signal (NLS) and two AT-hook motifs mediated non-specific DNA-binding, and DNA-binding deficient mutants retained the ability to efficiently stimulate HIV-1 integrase activity in vitro. Chromatin-association was assessed by visualizing the localization of EGFP fusion proteins in interphase and mitotic cells. Although a conserved N-terminal PWWP domain was not required for binding to condensed mitotic chromosomes, its deletion subtly affected the nucleoplasmic distribution of the protein during interphase. A dual AT-hook mutant associated normally with chromatin, yet when the mutations were combined with NLS changes or deletion of the PWWP domain, chromatin-binding function was lost. As the PWWP domain did not readily bind free DNA in vitro, our results indicate that chromatin-association is primarily affected through DNA-binding, with the PWWP domain likely contributing a protein interaction to the overall affinity of LEDGF/p75 for human chromatin. (+info)DNA binding properties of TAF1 isoforms with two AT-hooks. (7/23)
TATA-binding protein-associated factor 1 (TAF1) is an essential component of the general transcription factor IID (TFIID), which nucleates assembly of the preinitiation complex for transcription by RNA polymerase II. TATA-binding protein and TAF1.TAF2 heterodimers are the only components of TFIID shown to bind specific DNA sequences (the TATA box and initiator, respectively), raising the question of how TFIID localizes to gene promoters that lack binding sites for these proteins. Here we demonstrate that Drosophila TAF1 protein isoforms TAF1-2 and TAF1-4 directly bind DNA independently of TAF2. DNA binding by TAF1 isoforms is mediated by cooperative interactions of two identical AT-hook motifs, one of which is encoded by an alternatively spliced exon. Electrophoretic mobility shift assays revealed that TAF1-2 bound the minor groove of adenine-thymine-rich DNA with a preference for the sequence AAT. Alanine-scanning mutagenesis of the alternatively spliced AT-hook indicated that Lys and Arg residues made essential DNA contacts, whereas Gly and Pro residues within the Arg-Gly-Arg-Pro core sequence were less important for DNA binding, suggesting that AT-hooks are more divergent than previously predicted. TAF1-2 bound with variable affinity to the transcription start site of several Drosophila genes, and binding to the hsp70 promoter was reduced by mutation of a single base pair at the transcription start site. Collectively, these data indicate that AT-hooks serve to anchor TAF1 isoforms to the minor groove of adenine-thymine-rich Drosophila gene promoters and suggest a model in which regulated expression of TAF1 isoforms by alternative splicing contributes to gene-specific transcription. (+info)Pituitary transcription factor Prop-1 stimulates porcine pituitary glycoprotein hormone alpha subunit gene expression. (8/23)
Recently, we have reported that a Prophet of Pit-1 homeodomain factor, Prop-1, is a novel transcription factor for the porcine follicle-stimulating hormone beta subunit (FSHbeta) gene. This study subsequently aimed to examine the role of Prop-1 in the gene expression of two other porcine gonadotropin subunits, pituitary glycoprotein hormone alpha subunit (alphaGSU), and luteinizing hormone beta subunit (LHbeta). A series of deletion mutants of the porcine alphaGSU (up to -1059 bp) and LHbeta (up to -1277 bp) promoters were constructed in the reporter vector, fused with the secreted alkaline phosphatase gene (pSEAP2-Basic). Transient transfection studies using GH3 cells were carried out to estimate the activation of the porcine alphaGSU and LHbeta promoters by Prop-1, which was found to activate the alphaGSU promoter of -1059/+12 bp up to 11.7-fold but not the LHbeta promoter. Electrophoretic mobility shift assay and DNase I footprinting analysis revealed that Prop-1 binds to six positions, -1038/-1026, -942/-928, -495/-479, -338/-326, -153/-146, and -131/-124 bp, that comprise the A/T cluster. Oligonucleotides of six Prop-1 binding sites were directly connected to the minimum promoter of alphaGSU, fused in the pSEAP2-Basic vector, followed by transfecting GH3 cells to determine the cis-acting activity. Finally, we concluded that at least five Prop-1 binding sites are the cis-acting elements for alphaGSU gene expression. The present results revealed a notable feature of the proximal region, where three Prop-1-binding sites are close to and/or overlap the pituitary glycoprotein hormone basal element, GATA-binding element, and junctional regulatory element. To our knowledge, this is the first demonstration of the role of Prop-1 in the regulation of alphaGSU gene expression. These results, taken together with our previous finding that Prop-1 is a transcription factor for FSHbeta gene, confirm that Prop-1 modulates the synthesis of FSH at the transcriptional level. On the other hand, the defects of Prop-1 are known to cause dwarfism and combined pituitary hormone deficiency accompanying hypogonadism. Accordingly, the present observations provide a novel view to understand the hypogonadism caused by Prop-1 defects at the molecular level through the regulatory mechanism of alphaGSU and FSHbeta gene expressions. (+info)AT-hook motifs are short DNA-binding domains that are found in many eukaryotic transcription factors and other proteins that interact with chromatin. These motifs are typically composed of 6-8 amino acid residues, characterized by the presence of a highly conserved tripeptide sequence (PWK, PWV, or PWY), which is responsible for their ability to bind to the minor groove of AT-rich DNA sequences.
The AT-hook motifs can bend and kink the DNA helix, leading to changes in chromatin structure and modulation of gene expression. They play important roles in various nuclear processes, including transcriptional regulation, DNA replication, and repair. The presence of multiple AT-hook motifs in a single protein can enhance its DNA-binding affinity and specificity, allowing it to interact with specific regulatory elements in the genome.
Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.
Amino acid motifs are recurring patterns or sequences of amino acids in a protein molecule. These motifs can be identified through various sequence analysis techniques and often have functional or structural significance. They can be as short as two amino acids in length, but typically contain at least three to five residues.
Some common examples of amino acid motifs include:
1. Active site motifs: These are specific sequences of amino acids that form the active site of an enzyme and participate in catalyzing chemical reactions. For example, the catalytic triad in serine proteases consists of three residues (serine, histidine, and aspartate) that work together to hydrolyze peptide bonds.
2. Signal peptide motifs: These are sequences of amino acids that target proteins for secretion or localization to specific organelles within the cell. For example, a typical signal peptide consists of a positively charged n-region, a hydrophobic h-region, and a polar c-region that directs the protein to the endoplasmic reticulum membrane for translocation.
3. Zinc finger motifs: These are structural domains that contain conserved sequences of amino acids that bind zinc ions and play important roles in DNA recognition and regulation of gene expression.
4. Transmembrane motifs: These are sequences of hydrophobic amino acids that span the lipid bilayer of cell membranes and anchor transmembrane proteins in place.
5. Phosphorylation sites: These are specific serine, threonine, or tyrosine residues that can be phosphorylated by protein kinases to regulate protein function.
Understanding amino acid motifs is important for predicting protein structure and function, as well as for identifying potential drug targets in disease-associated proteins.
An amino acid sequence is the specific order of amino acids in a protein or peptide molecule, formed by the linking of the amino group (-NH2) of one amino acid to the carboxyl group (-COOH) of another amino acid through a peptide bond. The sequence is determined by the genetic code and is unique to each type of protein or peptide. It plays a crucial role in determining the three-dimensional structure and function of proteins.
A nucleotide motif is a specific sequence or pattern of nucleotides (the building blocks of DNA and RNA) that has biological significance. These motifs can be found in various contexts, such as within a gene, regulatory region, or across an entire genome. They may play a role in regulating gene expression, DNA replication, repair, or other cellular processes.
For example, in the context of DNA, a simple nucleotide motif could be a palindromic sequence (e.g., "CGGCGG") that can form a hairpin structure during transcription or translation. More complex motifs might include cis-regulatory elements, such as promoters, enhancers, or silencers, which contain specific arrangements of nucleotides that interact with proteins to control gene expression.
In the context of RNA, nucleotide motifs can be involved in various post-transcriptional regulatory mechanisms, such as splicing, localization, stability, and translation. For instance, stem-loop structures or specific sequence elements within RNA molecules might serve as recognition sites for RNA-binding proteins or non-coding RNAs (e.g., microRNAs) that modulate RNA function.
Overall, nucleotide motifs are essential components of the genetic code and play crucial roles in shaping gene expression and cellular functions.
Flagella are long, thin, whip-like structures that some types of cells use to move themselves around. They are made up of a protein called tubulin and are surrounded by a membrane. In bacteria, flagella rotate like a propeller to push the cell through its environment. In eukaryotic cells (cells with a true nucleus), such as sperm cells or certain types of algae, flagella move in a wave-like motion to achieve locomotion. The ability to produce flagella is called flagellation.
A base sequence in the context of molecular biology refers to the specific order of nucleotides in a DNA or RNA molecule. In DNA, these nucleotides are adenine (A), guanine (G), cytosine (C), and thymine (T). In RNA, uracil (U) takes the place of thymine. The base sequence contains genetic information that is transcribed into RNA and ultimately translated into proteins. It is the exact order of these bases that determines the genetic code and thus the function of the DNA or RNA molecule.
In the context of medical and biological sciences, a "binding site" refers to a specific location on a protein, molecule, or cell where another molecule can attach or bind. This binding interaction can lead to various functional changes in the original protein or molecule. The other molecule that binds to the binding site is often referred to as a ligand, which can be a small molecule, ion, or even another protein.
The binding between a ligand and its target binding site can be specific and selective, meaning that only certain ligands can bind to particular binding sites with high affinity. This specificity plays a crucial role in various biological processes, such as signal transduction, enzyme catalysis, or drug action.
In the case of drug development, understanding the location and properties of binding sites on target proteins is essential for designing drugs that can selectively bind to these sites and modulate protein function. This knowledge can help create more effective and safer therapeutic options for various diseases.
Sequence homology, amino acid, refers to the similarity in the order of amino acids in a protein or a portion of a protein between two or more species. This similarity can be used to infer evolutionary relationships and functional similarities between proteins. The higher the degree of sequence homology, the more likely it is that the proteins are related and have similar functions. Sequence homology can be determined through various methods such as pairwise alignment or multiple sequence alignment, which compare the sequences and calculate a score based on the number and type of matching amino acids.
Protein binding, in the context of medical and biological sciences, refers to the interaction between a protein and another molecule (known as the ligand) that results in a stable complex. This process is often reversible and can be influenced by various factors such as pH, temperature, and concentration of the involved molecules.
In clinical chemistry, protein binding is particularly important when it comes to drugs, as many of them bind to proteins (especially albumin) in the bloodstream. The degree of protein binding can affect a drug's distribution, metabolism, and excretion, which in turn influence its therapeutic effectiveness and potential side effects.
Protein-bound drugs may be less available for interaction with their target tissues, as only the unbound or "free" fraction of the drug is active. Therefore, understanding protein binding can help optimize dosing regimens and minimize adverse reactions.
Tertiary protein structure refers to the three-dimensional arrangement of all the elements (polypeptide chains) of a single protein molecule. It is the highest level of structural organization and results from interactions between various side chains (R groups) of the amino acids that make up the protein. These interactions, which include hydrogen bonds, ionic bonds, van der Waals forces, and disulfide bridges, give the protein its unique shape and stability, which in turn determines its function. The tertiary structure of a protein can be stabilized by various factors such as temperature, pH, and the presence of certain ions. Any changes in these factors can lead to denaturation, where the protein loses its tertiary structure and thus its function.
In genetics, sequence alignment is the process of arranging two or more DNA, RNA, or protein sequences to identify regions of similarity or homology between them. This is often done using computational methods to compare the nucleotide or amino acid sequences and identify matching patterns, which can provide insight into evolutionary relationships, functional domains, or potential genetic disorders. The alignment process typically involves adjusting gaps and mismatches in the sequences to maximize the similarity between them, resulting in an aligned sequence that can be visually represented and analyzed.
A conserved sequence in the context of molecular biology refers to a pattern of nucleotides (in DNA or RNA) or amino acids (in proteins) that has remained relatively unchanged over evolutionary time. These sequences are often functionally important and are highly conserved across different species, indicating strong selection pressure against changes in these regions.
In the case of protein-coding genes, the corresponding amino acid sequence is deduced from the DNA sequence through the genetic code. Conserved sequences in proteins may indicate structurally or functionally important regions, such as active sites or binding sites, that are critical for the protein's activity. Similarly, conserved non-coding sequences in DNA may represent regulatory elements that control gene expression.
Identifying conserved sequences can be useful for inferring evolutionary relationships between species and for predicting the function of unknown genes or proteins.
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Moss Gown by William H. Hooks
Steel Crochet Hook2
- Steel Crochet Hook No. 10 or 11. (freevintagecrochet.com)
- Size 50, with Milwards Steel Crochet Hook No. 12. (freevintagecrochet.com)
Crochet7
- What a wealth of stitch patterns using bedspread cotton and steel size 10 crochet hook. (frugalhaus.com)
- These lace-type crochet motifs remind me spiderwebs. (allfreecrochet.com)
- You can crochet these motifs with your favorite Halloween colors. (allfreecrochet.com)
- Fully lined, zippered bag is worked with two strands size 5 crochet cotton and one strand metallic blending filament using a size D/3 crochet hook. (freepatterns.com)
- All you need for this project is some worsted cotton yarn, a size k crochet hook, and a removable stitch marker. (crochetme.com)
- Granny squares are a popular crochet motif, but have you tried a granny triangle? (vogueknittinglive.com)
- You can use any color you want, but a gradient or color changing yarn makes the crochet motif stand out. (vogueknittinglive.com)
Rnds3
- Rep Rnds 1-2 of Motif # 1. (maggiescrochet.com)
- SECOND MOTIF … Work same as First Motif until 5 rnds are completed. (freevintagecrochet.com)
- SECOND MOTIF … Work 1st 6 rnds as for first motif. (freevintagecrochet.com)
Proteins6
- All the AHL proteins were further classified into three types (I, II and III) based on the AT-hook motif. (biomedcentral.com)
- AT-hook motifs identified in a wide variety of DNA-binding proteins. (nih.gov)
- For a more-detailed investigation on the interaction of HMGA1a with chromatin, the contribution of the AT-hook DNA-binding motifs was analyzed using point-mutated HMGA1a-GFP proteins. (biologists.com)
- Collectively our data show that the kinetic properties of HMGA1a proteins are governed by the number of functional AT-hooks and are regulated by specific phosphorylation patterns. (biologists.com)
- Many of the SNAG motif-containing proteins regulate EMT in developmental stages and also in cancerous cells. (eu.org)
- Near the amino terminus, it has a DNA methyltransferase domain and three adenine-thymine hook motifs similar to those originally discovered in high-mobility group proteins and implicated in minor groove DNA binding (Figure 1). (medscape.com)
Second Motif4
- Make 32 motifs in all, joining them as Second Motif was joined to First Motif and placing them as illustrated. (freevintagecrochet.com)
- Sl st in each of next 4 d c, ch 3, d c in next 2 d c, 2 d c in next sp, ch 1, sl st in a corner sp of first motif, ch 1, 2 d c back in same sp on second motif as last 2 d c were made. (freevintagecrochet.com)
- Ch 1, 2 d c back in same sp on second motif as last 2 d c were made, and complete rnd as for first motif (no more joinings). (freevintagecrochet.com)
- Make necessary number of motifs (see diagram) and join as second motif was joined to first (where 3 corners meet, join 3rd corner to joining of other 2 corners). (freevintagecrochet.com)
Joinings2
- Complete rnd as for First Motif (no more joinings). (freevintagecrochet.com)
- Join 3rd motif to 2nd motif in same manner leaving 4 picots free between joinings. (freevintagecrochet.com)
Earrings3
- Find more inspiring earring tops in our extensive collection of hook tops, hoops, clip-ons, and post earrings. (ninadesigns.com)
- Artisan Rosario Leiva of Guatemala brings new life to recycled CDs in these beautiful and fascinating dangle earrings with sterling silver hooks. (novica.com)
- Balinese artisan Janice Girardi designs these dangle earrings with a humanitarian concern for animals, adding sterling hooks that host the stones. (shopzilla.com)
Squares1
- A Balouch rug, the shaded indigo field with overall diagonal octagon lattice containing hooked guls and small squares with 'S' motifs surrounded by brick-red angular hooked vine and hooked motif border betwee zig-zag and floral stripes, stylised skirt at each end, area of slight wear --5ft.4in. (christies.com)
Thrombospondin2
- HN - 2017 MH - ADAMTS1 Protein UI - D000071097 MN - D8.811.277.656.675.374.102.500.500 MN - D9.400.430.500.500.500 MN - D12.776.395.33.500.500 MN - D12.776.860.300.85.500 MS - An ADAMTS protease that contains two disintegrin loops and three C-terminal thrombospondin (TS) motifs. (nih.gov)
- HN - 2017 MH - ADAMTS13 Protein UI - D000071120 MN - D8.811.277.656.675.374.102.500.813 MN - D9.400.430.500.500.813 MN - D12.776.395.33.500.813 MN - D12.776.860.300.85.813 MS - An ADAMTS protease that contains eight thrombospondin (TS) motifs. (nih.gov)
Highly conserved4
- Previous research has shown that the AT-Hook Motif Nuclear Localized ( AHL ) gene family is highly conserved in land plants, playing crucial roles in plant growth and development. (biomedcentral.com)
- The AT-Hook Motif Nuclear Localized (AHL) gene family is highly conserved across all land plants, and the AHL transcription factors were previously described in mosses and flowering plants [ 1 ]. (biomedcentral.com)
- The AT-hook motif gene family is highly conserved across plant species and plays relevant roles during plant development. (biomedcentral.com)
- The minimal SNAG motif is a circa eight amino acid length sequence highly conserved in the very N-terminal region of these TFs, with the N-terminal methionine cleaved off as usual. (eu.org)
Transcriptional3
- In many transcriptional repression mechanisms, the SNAG motif of the corresponding transcriptional factors brings this complex to its target gene promoters where it binds a specific hexanucleotide sequence through the zinc-finger motifs of the transcription factors and represses the gene expression. (eu.org)
- 9. Disruption of the architectural factor HMGI-C: DNA-binding AT hook motifs fused in lipomas to distinct transcriptional regulatory domains. (nih.gov)
- A polymorphism in the AT-hook motif of the transcriptional regulator AKNA is a risk factor for cervical cancer. (cdc.gov)
Dangle1
- There's plenty of room for creativity with this romantic and feminine leaf motif ear wire - suspend your favorite gemstone or dangle. (ninadesigns.com)
Yarn1
- Each motif measures about 4.5" wide and requires less than 12 yards of #2-fine/sport weight yarn. (allfreecrochet.com)
Loop4
- Non-canonical splicing motifs CNG'CNG in the loop region of H2 and H3 hairpins are conserved. (wikipedia.org)
- Stem-loop structures around the splice sites and IRE1-specific sequence motifs are both necessary and sufficient for splicing to occur. (wikipedia.org)
- make a cluster in any loop on next motif. (freevintagecrochet.com)
- ch 5, skip 1 loop, make another joint st by making dc in next loop and tr in fol-lowing loop, ch 5, cluster in next loop, ch 8, make a joint-tr by making tr in last loop on this motif and in first loop on next motif, ch 8, 3-tr cluster in next loop. (freevintagecrochet.com)
Needle1
- Scissors, tapestry needle, 2 different colored yarns, and two different sized hooks will be required. (crochetme.com)
Decorate1
- Various stellar motifs decorate the leaf. (rmg.co.uk)
Size1
- For Hook Counts, see our Size Chart. (herroom.com)
Cluster2
- thread over and draw through all loops on hook (a cluster made). (freevintagecrochet.com)
- Join last cluster on last motif to tip of first cluster on first motif. (freevintagecrochet.com)
Floral1
- Floral motif, removable elastic straps in front are 7/8" wide and adjust in back on 3/8" wide elastic with plastic hardware. (herroom.com)
Make6
- We tell you how many motifs to make for 3 sizes of cloths. (freevintagecrochet.com)
- 1-300 yard ball will make 10 motifs. (freevintagecrochet.com)
- 22-300 yard balls will make a cloth 13 x 17 motifs. (freevintagecrochet.com)
- Make as many motifs as you want and string them into a garland. (allfreecrochet.com)
- Make necessary number of motifs (1 motif will make a 2-inch scallop on completed edging). (freevintagecrochet.com)
- Join 12 sunflower motifs to make this pretty gusseted purse. (freepatterns.com)
Sequence1
- The SNAG motif shows partial similarity to the Histone H3 N-terminal sequence and binds to the LSD1 active site in a conformation similar to that of the H3 tail. (eu.org)
GAUGE1
- GAUGE … Each motif measures 3 inches from side to side (across center) before blocking. (freevintagecrochet.com)
Architectural1
- She integrates architectural motifs into bodily shapes - segmented and layered, organic and geometric - that are caught among dense scaffolding and rubble. (culturemap.com)
Join1
- Join 4th motif to 1st and 3rd motif. (freevintagecrochet.com)
Center2
- FIRST MOTIF … Starting at center, ch 8. (freevintagecrochet.com)
- MOTIF … Starting at center with Robinette, ch 10. (freevintagecrochet.com)
Measures1
- Each motif measures about 2 inches square. (freevintagecrochet.com)
Roles1
- The AT-hook motif in SA1 plays dual roles in modulating non-specific DNA binding and subdiffusive dynamics over telomeric regions. (nih.gov)
Sterling silver1
- One carat of glistening oval blue topaz stones is embraced by sterling silver paw print motifs. (shopzilla.com)
Domain3
- We identified a total of 63 AT-hook motif genes, which were characterized by the presence of the AT-hook motif and PPC domain in soybean. (biomedcentral.com)
- The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. (cancerindex.org)
- SNAG is actually a short linear motif, not a globular domain). (eu.org)
Gene5
- Hence, the AHL gene family mainly reacts on mediating stress responses in the roots and provide comprehensive information for further understanding of the AT-hook motif gene family-mediated stress response in soybean. (biomedcentral.com)
- The AT-hook motif gene family is involved in in very important biological processes in plants. (biomedcentral.com)
- Moreover, the AT-hook motif gene family is also able to regulates the expression of cell-specific genes. (biomedcentral.com)
- Vertebrate LSD1 is recruited to target gene promoters by interacting with the SNAG motif present in various transcription factors such as SNAIL family members, Gfi-1, Insm1 and Ovo‐like 1 (OVOL1). (eu.org)
- 17. Point mutations within AT-hook domains of the HMGI homologue HMGIYL1 affect binding to gene promoter but not to four-way junction DNA. (nih.gov)
Work1
- it's not a complicated motif but you will need to position it carefully and work the knot effect neatly. (letsknit.co.uk)
Functions1
- This motif is mainly observed among vertebrate zinc finger and homeobox transcription factors and accounts for repressive functions and protein stability. (eu.org)
Brass1
- Robe Hook is constructed of the finest solid brass materials to provide a sturdy hook for your robes and towels. (kitchensource.com)
Repeat1
- Repeat from * until all motifs have been joined. (freevintagecrochet.com)
Present2
Musical1
- Such musical motifs can be a hook in their own right - you need to think melodically and try to compose simple, memorable phrases that enhance the song without stealing the lead vocal's thunder. (soundonsound.com)