A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
The degree of replication of the chromosome set in the karyotype.
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).
DNA present in neoplastic tissue.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
A scanning microscope-based, cytofluorimetry technique for making fluorescence measurements and topographic analysis on individual cells. Lasers are used to excite fluorochromes in labeled cellular specimens. Fluorescence is detected in multiple discrete wavelengths and the locational data is processed to quantitatively assess APOPTOSIS; PLOIDIES; cell proliferation; GENE EXPRESSION; PROTEIN TRANSPORT; and other cellular processes.
Study of the scientific principles, mechanisms, and effects of the interaction of ionizing radiation with living matter. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The measurement of radiation by photography, as in x-ray film and film badge, by Geiger-Mueller tube, and by SCINTILLATION COUNTING.
The amount of radiation energy that is deposited in a unit mass of material, such as tissues of plants or animal. In RADIOTHERAPY, radiation dosage is expressed in gray units (Gy). In RADIOLOGIC HEALTH, the dosage is expressed by the product of absorbed dose (Gy) and quality factor (a function of linear energy transfer), and is called radiation dose equivalent in sievert units (Sv).
The sorting out and classification of patients or casualties to determine priority of need and proper place of treatment.
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
The study of those aspects of energy and matter in terms of elementary principles and laws. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Harmful effects of non-experimental exposure to ionizing or non-ionizing radiation in VERTEBRATES.
The voltage difference, normally maintained at approximately -180mV, across the INNER MITOCHONDRIAL MEMBRANE, by a net movement of positive charge across the membrane. It is a major component of the PROTON MOTIVE FORCE in MITOCHONDRIA used to drive the synthesis of ATP.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
The two lipoprotein layers in the MITOCHONDRION. The outer membrane encloses the entire mitochondrion and contains channels with TRANSPORT PROTEINS to move molecules and ions in and out of the organelle. The inner membrane folds into cristae and contains many ENZYMES important to cell METABOLISM and energy production (MITOCHONDRIAL ATP SYNTHASE).
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
Experimentation on STEM CELLS and on the use of stem cells.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
Temporary visual deficit or impaired visual processing occurring in a rapid serial visual presentation task. After a person identifies the first of two visual targets, the ability to detect the second target is impaired for the next few hundred milliseconds. This phenomenon is called attentional blink.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
An inhibitory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 PROTEIN. CD274 antigen provides negative signals that control and inhibit T-cell responses and is found at higher than normal levels on tumor cells, suggesting its potential role in TUMOR IMMUNE EVASION.
An inhibitory T-lymphocyte receptor that has specificity for CD274 ANTIGEN and PROGRAMMED CELL DEATH 1 LIGAND 2 PROTEIN. Signaling by the receptor limits T cell proliferation and INTERFERON GAMMA synthesis. The receptor also may play an essential role in the regulatory pathway that induces PERIPHERAL TOLERANCE.
Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.
A costimulatory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 RECEPTOR. It is closely-related to CD274 antigen; however, its expression is restricted to DENDRITIC CELLS and activated MACROPHAGES.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Antibodies produced by a single clone of cells.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A publication issued at stated, more or less regular, intervals.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
Tumors or cancer of the COLON.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
A family of proteins that are structurally-related to Ubiquitin. Ubiquitins and ubiquitin-like proteins participate in diverse cellular functions, such as protein degradation and HEAT-SHOCK RESPONSE, by conjugation to other proteins.
A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.
A cell line derived from cultured tumor cells.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS.
A family of histone demethylases that share a conserved Jumonji C domain. The enzymes function via an iron-dependent dioxygenase mechanism that couples the conversion of 2-oxoglutarate to succinate to the hydroxylation of N-methyl groups.
... flow cytometry because it requires the dissociation of spheroids into a single-cell suspension, and the automation of liquid ... 2017). "A 1536-Well 3D Viability Assay to Assess the Cytotoxic Effect of Drugs on Spheroids". SLAS Discov. 22 (5): 516-524. doi ... Further challenges associated with complex 3D cell culture techniques include: imaging due to large scaffold sizes and ... but the available assays are not optimized for 3D cell culturing. Another challenge faced by 3D cell culturing is the limited ...
Edwards BS, Oprea T, Prossnitz ER, Sklar LA (August 2004). "Flow cytometry for high-throughput, high-content screening". Curr ... and Scanning for High-Throughput Assay. Molecular Imaging & Biology. 2018;20(3):368-377. doi:10.1007/s11307-017-1152-0 Mezencev ... Milligan G (July 2003). "High-content assays for ligand regulation of G-protein-coupled receptors". Drug Discov. Today. 8 (13 ... High-content screening technology is mainly based on automated digital microscopy and flow cytometry, in combination with IT- ...
... flow cytometry MeSH E05.200.500.386.400 - image cytometry MeSH E05.200.500.386.400.500 - laser scanning cytometry MeSH E05.200. ... drug screening assays, antitumor MeSH E05.337.550.200.800 - tumor stem cell assay MeSH E05.337.550.200.900 - xenograft model ... flow cytometry MeSH E05.196.712.516.600.240.400 - image cytometry MeSH E05.196.712.516.600.240.400.500 - laser scanning ... colony-forming units assay MeSH E05.200.500.383.910 - tumor stem cell assay MeSH E05.200.500.385 - cytogenetic analysis MeSH ...
Drug discovery High-throughput screening Drug discovery hit to lead Microscopy Flow cytometry Live single-cell imaging Haney SA ... some also have humidity control for longer term live cell imaging), a built in pipettor or injector for fast kinetic assays, ... Giuliano KA, Haskins JR, Taylor DL (August 2003). "Advances in high content screening for drug discovery". Assay Drug Dev ... Edwards BS, Oprea T, Prossnitz ER, Sklar LA (August 2004). "Flow cytometry for high-throughput, high-content screening". Curr ...
... single-particle interferometric reflectance imaging (down to about 40 nm), and nano-flow cytometry (also to 40 nm). Some ... Assaying for depleted markers is thought to be particularly important, as otherwise the purity of an EV preparation cannot be ... Exosome Diagnostics has developed several cancer diagnostic assays based in part on EV RNA.[citation needed] Codiak Biosciences ... electron microscopy and flow cytometry. To demonstrate the presence of EVs in a preparation, as well as the relative depletion ...
Flow-FISH uses flow cytometry to perform FISH automatically using per-cell fluorescence measurements. Microfluidics-assisted ... or imaged simultaneously with a fluorescent antibody assay. The technology has potential applications in cancer diagnosis, ... Some assays are designed so that the secondary color will be present or absent in cases of interest. An example is the ... Separate but compatible signal amplification systems enable the multiplex assay (up to two targets per assay). Signal ...
... optic Flow cytometric cell or particle counters, or coulter/impedance principle based cell counters Imaging assays, that ... involve image analysis manually or by software: Cytometry: When the size statistics of cells is assessed by an image processor ... DNase footprinting assay Filter binding assay Gel shift assay Bicinchoninic acid assay (BCA assay) Bradford protein assay Lowry ... Chemotaxis assay Secretion assays Apoptosis assays such as the DNA laddering assay, the Nicoletti assay, caspase activity ...
... counter Cytometry Dielectrophoresis Flow Cytometry Standard Mass cytometry Microfluorimetry Viability assay Flow Cytometry in ... Imaging flow cytometry (IFC) captures multichannel images of cells. Detectors used in imaging platforms can be equipped with ... Flow cytometry is widely applied to detect sperm cells abnormality associated with DNA fragmentation in male fertility assays. ... A flow cytometer is similar to a microscope, except that, instead of producing an image of the cell, flow cytometry offers high ...
... flow cytometry. FDAA consists of a D-amino acid and a fluorophore (coupled through the amino acid side chain.) The D-amino acid ... Assaying the anti-cell wall ability of antibiotics. Screening new anti-cell wall antibiotics. Tracking transpeptidase activity ... Radkov, Atanas D.; Hsu, Yen-Pang; Booher, Garrett; VanNieuwenhze, Michael S. (2018-06-20). "Imaging Bacterial Cell Wall ... "Fluorogenic d-amino acids enable real-time monitoring of peptidoglycan biosynthesis and high-throughput transpeptidation assays ...
Hult, Annika K; Frame, Tim; Chesla, Scott; Henry, Stephen; Olsson, Martin L (2012). "Flow cytometry evaluation of red blood ... These have been used to deposit FSL constructs onto microplates to create diagnostic assays. They also have the potential for ... Consequently, the constructs are compatible in vivo use, and can improve diagnostic assay sensitivity by allowing for the use ... "Fluorescent Function-Spacer-Lipid construct labelling allows for real-time in vivo imaging of cell migration and behaviour in ...
"Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device". ... If the physical barrier is removable (such as in PDMS or hydrogel), the assay can also be used to study cell invasion or cell ... Some of the challenges include: imaging of the cells, control of gradients in static models (i.e., without a perfusion system ... "Continuous perfusion microfluidic cell culture array for high-throughput cell-based assays". Biotechnology and Bioengineering. ...
... stemming off of the work done to sort cells in Flow Cytometry. Within the realm of droplet sorting there are two main types, ... Gu S, Lu Y, Ding Y, Li L, Zhang F, Wu Q (September 2013). "Droplet-based microfluidics for dose-response assay of enzyme ... January 2017). "An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in ... Kintses B, Hein C, Mohamed MF, Fischlechner M, Courtois F, Lainé C, Hollfelder F (August 2012). "Picoliter cell lysate assays ...
Antibodies are used in flow cytometry to differentiate cell types by the proteins they express; different types of cell express ... 1994). "Enzyme-linked immunosorbent assay (ELISA)". Methods Mol Biol. 32: 461-466. doi:10.1385/0-89603-268-X:461. ISBN 0-89603- ... "Animated depictions of how antibodies are used in ELISA assays". Cellular Technology Ltd.-Europe. Archived from the original on ... Immunofluorescence image of the eukaryotic cytoskeleton. Microtubules as shown in green, are marked by an antibody conjugated ...
"Standardization of cytokine flow cytometry assays". BMC Immunology. 6: 13. doi:10.1186/1471-2172-6-13. PMC 1184077. PMID ... Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI. 6859: 17. Bibcode:2008SPIE.6859E..17L. CiteSeerX ... coupled with the development of high-throughput robotic platforms capable of assaying hundreds to thousands of samples ... Flow Cytometry Standard (FCS) was developed in 1984 to allow recording and sharing of flow cytometry data. Since then, FCS ...
Hult, Annika K; Frame, Tim; Chesla, Scott; Henry, Stephen; Olsson, Martin L (2012). "Flow cytometry evaluation of red blood ... Georgakopoulos, T; Komarraju, Sarvani; Henry, Stephen; Bertolini, Joseph (2011). "An improved Fc function assay utilising CMV ... "Fluorescent Function-Spacer-Lipid construct labelling allows for real-time in vivo imaging of cell migration and behaviour in ... spacer from 1.9 to 7.2 nm it has been shown sensitivity can improve two-fold in red cell agglutination based kodecyte assays. ...
Microfluidic flow enables fast sample throughput, automated imaging of large sample populations, as well as 3D capabilities. or ... "Simultaneous Acoustic and Photoacoustic Microfluidic Flow Cytometry for Label-free Analysis". Sci. Rep. 9 (1): 1585. Bibcode: ... Uram, J. D.; Ke, K.; Hunt, A. J.; Mayer, M. (2006). "Label-free affinity assays by rapid detection of immune complexes in ... Yetisen AK, Jiang L, Cooper JR, Qin Y, Palanivelu R, Zohar Y (May 2011). "A microsystem-based assay for studying pollen tube ...
Miltenyi Biotec (2017-03-22), Webinar: Recombinant Antibodies for Improved Flow Cytometry, retrieved 2017-08-20 CS1 maint: ... To determine the binding activity, ELISA assay is routinely performed. Structurally Fab fragments consist of two sets of ... Bio-Rad Laboratories (2013-12-03), Developing Recombinant Anti Idiotypic Antibodies for PK/PD and Immunogenicity Assays, ... "Clearance properties of nano-sized particles and molecules as imaging agents: considerations and caveats". Nanomedicine. 3 (5 ...
... flow cytometry, and molecular-pathologic analysis.[9] One of the greatest challenges of dermatopathology is its scope. More ... Examples of important subdivisions in medical imaging include radiology (which uses the imaging technologies of X-ray ... antibody based immunofluorescence tissue assays, molecular profiling of pathogens, and analysis of bacterial genes for ... tactile imaging, thermography, medical photography, nuclear medicine and functional imaging techniques such as positron ...
Less fastidious, scalable, methods include the use of cytometers, while flow cytometry allows combining cell counts ('events') ... The MTT assays (colorimetric) and the resazurin assay (fluorimetric) dose the mitochondrial redox potential. ... Image result for cell growth Cell growth (or interphase) is shorthand for the idea of "growth in cell populations" by means of ... All these assays may correlate well, or not, depending on cell growth conditions and desired aspects (activity, proliferation ...
... enrichment-free assays that identify all CTC subtypes in sufficiently high definition to satisfy diagnostic pathology image- ... ViewRNA assay for CTCs characterization is the only in situ hybridization technology that allows multiplex, single-molecule RNA ... Cytometry Part A. 71A (3): 132-142. doi:10.1002/cyto.a.20369. PMID 17200956.. ... "Computational Analysis of Microfluidic Immunomagnetic Rare Cell Separation from a Particulate Blood Flow". Analytical ...
Enzyme assay. *Mass spectrometry. *Chromatography. *Flow cytometry. *Blood bank. *Microbiological culture. *Serology ... Other less common techniques (such as X-rays, CAT scans, PET scans or NMR) are used to produce images of internal abnormalities ... Polymerase chain reaction (PCR) assays are the most commonly used molecular technique to detect and study microbes.[29] As ... as this method provides faster data compared to a standard PCR assay. For instance, traditional PCR techniques require the use ...
RABiT biodosimetry assays have been developed for protocols that include the CBMN assay (11, 12), the γ-H2AX assay (13-15) and ... Imaging flow cytometry (IFC), which combines the statistical power of traditional flow cytometry with the sensitivity and ... Automation of the in vitro micronucleus assay using the Imagestream® imaging flow cytometer. Cytometry Part A 2018; 93: 706-26 ... The potential for complete automated scoring of the cytokinesis block micronucleus cytome assay using imaging flow cytometry. ...
The in vitro micronucleus assay is a well-established method for evaluating genotoxicity and cytotoxicity but scoring the assay ... quality control and prospects for further development of automated micronucleus assays using image cytometry systems. ... In light of these limitations, Multispectral Imaging Flow Cytometry (MIFC) is an ideal system to perform the MN assay since it ... Rodrigues, M. A. Automation Of The In vitro Micronucleus Assay Using The ImageStream® Imaging Flow Cytometer. Cytometry Part A ...
Flow Cytometry) (ab112149). MOMP detection in FL4 channel. Ex/Em: 635/660 nm. Ideal for multi-parameter studies. ... Cell and tissue imaging tools. Cellular and biochemical assays. By product type. Proteins and Peptides. Proteomics tools. ... NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) ab112149 is designed to detect cell apoptosis by measuring the ... Custom assay development. We can help with downstream assay development for single-wash ELISA assays (SimpleStep ELISA®) and ...
8. Other Flow Cytometric Assays. 8.1 Reporter gene assay: beta-galactosidase measurement. FDG= fluorescein-di-beta- ... Cytometry 13:490-495.. Terry, N.H.A, R.A. White, M.L. Meistrich, and D.P. Calkins (1991) Evaluation of Flow Cytometric Methods ... Read flow immediately.. 6.4 Rhodamine-123 efflux: Functional assay. *1 x 106 cells are preincubated with verapamil (10 µg/mL) ... Cytometry 12:234-241.. Terry, N.H.A., R.A. White, and M.L. Meistrich () Cell Kinetics: from Titriated Thymidine to Flow ...
To ensure reliable enumeration results, the EPCs are enriched during the procedure to increase the sensitivity of the flow ... Standardized enumeration of human endothelial progenitor cells (EPCs) based on a flow cytometric assay. ... Data and images. Data and images for EPC Enrichment and Enumeration Kit, human. ... Cells were stained with CD34-FITC and CD133/2 (293C3)-PE and analyzed by flow cytometry. The population in R4 was used for ...
SARS-CoV-2 assays to detect functional antibody responses that block ACE2 recognition in vaccinated animals and infected ... In aged primary T cells mitochondrial stress contributes to telomere attrition measured by a novel imaging flow cytometry assay ... In aged primary T cells mitochondrial stress contributes to telomere attrition measured by a novel imaging flow cytometry assay ... In aged primary T cells mitochondrial stress contributes to telomere attrition measured by a novel imaging flow cytometry assay ...
An automated approach to genotoxicity studies based on deep learning and imaging flow cytometry eliminates subjectivity and ... enables assays to be performed with consistent results in labs around the world. ... Figure 6. A tSNE visualization of data showing typical cell types from the genotoxicity assay. ... Automating Genotoxicity Assays with Imaging Flow Cytometry and Deep Learning. By Paul Rees, Swansea University ...
Imaging Reagents. *Microplate Assays. *Flow Cytometry Reagents. *Secondary Detection Tools. *Alexa Fluor Dyes ... See the Invitrogen Attune Acoustic Focusing Cytometer and find antibodies, antibody conjugates, and assay kits. ... Discover the ideal assays to investigate your cells health and behavior using our application-based selection tools. ... Eliminate the complexities of microscopy without compromising performance-Invitrogen EVOS Cell Imaging Systems. ...
Imaging flow cytometry. MTR staining was performed as described earlier. Subsequently, cells (0.5 × 106) were fixed and stained ... indicates below the assay detection limit. (G) Percentage of IFN-γ+ cells among activated effector memory CD4+ T cells that ... These assays showed that neutralization of TGF-β in the effusion fluids increased the percentage of effector memory CD4+ T ... 5B). Next, having verified by imaging flow cytometry that ivermectin and SB-431542 both inhibited the nuclear import of pSmad2/ ...
... and were then either fixed for imaging assay (A, β1 inhibition; B, isotype control) or processed for flow cytometry to quantify ... Flow cytometry to quantify endocytosed fibronectin. After pulse-chase assays, cells were incubated with a mixture of 0.02% EDTA ... cells were either fixed for imaging assay (A, β1 inhibition; B, isotype control), or processed for flow cytometry to quantify ... Peter Keng and Timothy Bushnell for advice on flow cytometry, Keigi Fujiwara and Liam Casey for their help with image analysis ...
Microscopy Tissue Culture , Multi-Color Flow Cytometry , Assay Development , High-Content Imaging. Protein Expression & ... Serve as a hands-on technician performing molecular biology assays, including DNA isolation, DNA sequencing, PCR amplification ...
In conclusion, a functional assay evaluating antibodies targeting PD-1 inhibition in vitro revealed that pembrolizumab is a ... To date, therapeutic PD-1 inhibitors have not been compared in a functional assay. We used an efficient T cell reporter ... In addition, we used flow cytometry-based binding assays on the cells in our reporter system to explore whether it can be used ... The images or other third party material in this article are included in the articles Creative Commons license, unless ...
... stem and progenitor cells by flow cytometry (Fig. 2a and a), in vitro colony-forming units (CFU) assays revealed an increase ... Open image in new window. Fig. 4 Chronic intermittent hypoxia modifies the BM vascular structure. a-e Representative images ... In vitro colony forming assay. Femur BM cells were flushed with PBS 2 mM EDTA, treated with red blood cell lysis buffer ( ... Flow cytometry. Bone marrow and peripheral blood cells were treated with red blood cell lysis buffer (Biolegend) for 15 min in ...
Stained samples were tested by flow cytometry (ACEA NovoCyte, USA).. Confocal microscopy assay. The cells were seeded in a ... Colony formation assays. PANC-1 and SW1990 cells (1 × 103/well) were seeded in 12-well plates and cultured for 24 h. The medium ... The images or other third party material in this article are included in the articles Creative Commons license, unless ... E, F Flow cytometry was performed to measure mitochondrial ROS by mitoSOX probe (3 μM) (values represented mean ± SD. ★P , 0.05 ...
Mitochondrial Assay), Detection Technology (Flow Cytometry, Florescence microscopy), Application (Stem Cell, Discovery & ... Apoptosis Assays Market by Product (Caspase, Annexin V, DNA Fragmentation, ... On the basis of detection technology, the apoptosis assays market is segmented into flow cytometry, cell imaging & microscopy, ... Table APOPTOSIS ASSAYS MARKET, BY DETECTION TECHNOLOGY, 2016-2023 (USD MILLION). *FLOW CYTOMETRY*Table FLOW CYTOMETRY MARKET ...
Flow cytometry of CSF and whole blood cells was performed as previously described (71). All flow cytometry data were acquired ... Migration Assay.. Migration assays were performed as previously described (Fig. S2 and ref. 73). The confluence of the HBMEC ... Static Imaging of NK Cells.. Static imaging of CD56bright and CD56dim NK-cell subsets was performed as previously described (38 ... Flow cytometry of CSF and whole blood cells was performed as previously described (71). All flow cytometry data were acquired ...
D-G) Representative dot plot images of flow cytometry and quantitative analysis of CD45-CD31-Sca-1+CD24- APCs, CD45-CD31-Sca-1- ... Flow cytometry assay. Mouse femurs were dissected, and soft tissue of the femur was removed. We then crushed femurs with ... CFU-F, CFU-OB, and CFU-AD in vitro differentiation assays. Bone marrow digestion and CFU-F, CFU-OB and CFU-AD assays of mouse ... N-Q) Representative dot plot images of flow cytometry and quantitative analysis of CD45-CD31-Sca-1+CD24- APCs, CD45-CD31-Sca-1- ...
... specific CD8+ CTL frequencies in more complex cell populations from peripheral blood by comparing the assay with flow cytometry ... A significantly lower frequency of NA.75-specific CTL was obtained in both assays: 0.19% in the microarray assay compared to ... Image acquisition and data analysis. Microarray images were acquired using a ProScanArray fluorescence scanner (PerkinElmer, ... Flow cytometry measurements. The frequencies of antigen-specific CTLs in aAPC-enriched populations or in donor CD8 cells were ...
Abcam offers , 1,000 assay kits cited in , 3,500 publications. ... Assay Caspase Activity in cell samples with Generic Caspase ... Images. * Flow Cytometry - Generic Caspase Activity Assay Kit (Fluorometric - Red) (ab112131). Jurkat cells were untreated ( ... Cell and tissue imaging tools. Cellular and biochemical assays. Proteins and Peptides. By product type. Proteomics tools. ... Multiplex miRNA assays. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ...
Flow cytometry binding assay. In vitro binding studies of IRDye800CW-labeled 8708 and 8709 antibody fragments were performed ... Current EGFR diagnostic assays are invasive and suffer from a number of caveats. For example, to assess the status of an EGFR- ... Click on the image to enlarge.). Figure 3 8708 and 8709 fragment flow cytometry. 8708 and 8709 fragments were titrated with A- ... W.B. designed and performed experiments (labeling, flow cytometry, cell culture, xenografts and imaging), analyzed data and ...
In order to directly distinguish the M phase cells, flow cytometry assays were carried out with Propidium Iodide (PI) staining ... For CHIP assay, anti-DNMT3 (Abcam) was used to bind target protein-DNA complexes. The primers used in the 3C-ChIP-PCR assays ... Written informed consent was obtained from the individual(s) for the publication of any potentially identifiable images or data ... and flow cytometric analysis was performed on a BD FACSCalibur device (BD Biosciences). Flow cytometry was also used to confirm ...
A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine. Cytometry B Clin Cytom. ( ... Images were taken with a fluorescent microscopy (Zeiss AxioImages.A1, Carl Zeiss). In a single experiment at least 30 cells per ... Usually the repair defect in these disorders is assessed by immunofluorescence assays of irradiation-induced γ-H2AX foci using ... Flow cytometry (FC) can be applied as a rapid diagnostic tool for DNA repair disorders (17, 18). Therefore, we have used flow ...
To increase the sensitivity of our assay, in a separate cohort of mice we used PET scanning to image the mice in vivo to detect ... A) Total tumor cells were stained for CD45 and MART-1 tetramer and analyzed by flow cytometry to detect TILs. The data indicate ... Ex Vivo Functional Assays.. Splenocytes from treated and untreated BLT mice were cultured with irradiated (3,500 rads), peptide ... B) Progenitor cells were stimulated with SCF (50 ng/mL) and MGDF (100 ng/mL) and were used in a flow cytometric assay to detect ...
... high content imaging and organoid derivation. Collectively, the team provide cell biology expertise to help deliver some of the ... In this role she leads a team of 75 scientists covering a broad range of scientific areas including flow cytometry, CRISPR ... In this role she leads a team of 75 scientists covering a broad range of scientific areas including flow cytometry, CRISPR ... Alex has a background in cell and molecular biology, in particular the development of cell lines and assays for large scale or ...
Cell Biology, Molecular Biology, Flow Cytometry, Molecular Cloning, Public Speaking, Science Outreach, Scientific Writing, ... Advanced Imaging Cardiology. The University of Western Ontario 2007 - 2008. Advanced Imaging Cardiology. The University of ... Assay Development, Project Management, Molecular Biology, Cell, ELISA, Immunoassays, Cell Culture, High Throughput..., Cell ... Cell Based Assays, Biopharmaceuticals, Drug Discovery, Protein Purification, qPCR, Oncology, In Vitro, SDS-PAGE, Protein ...
Publishing Revolutionary Research Imaging flow cytometry makes it possible. *Chromatography Sample Preparation Maintain clean ... Neurite Outgrowth Quantification Assay. Mercks NS230 Neurite Outgrowth Assay Plus Kit (3 µm) utilizes microporous tissue ... Antibodies & Assays Trust in one of the most highly published & validated portfolios of antibodies. ...
TIP: Adhere to principles of proper flow cytometry panel design. For instance, label dim or critical targets with antibodies ... vFC™ VESICLE ANALYSIS ASSAYS. *vTAG™ ANTIBODIES. *VESICLES AND VESICLE ASSAY STANDARDS. *BULK AND CUSTOM PRODUCTS ... Image. Name. SKU. Price. Buy. vCAL™ ANTIBODY CAPTURE BEADS. CBS7. $95.00. - $350.00. Size. 250 ul (50 tests). 25 ul (5 tests). ... Image. Name. SKU. Price. Buy. vTAG™ ANTI-HUMAN CD235AB ANTIBODY. NO WASH, QUANTITATIVE CD235AB MEASUREMENT BY vFC™. CBS31. $ ...
ALDH1 activity assays. Aldehyde dehydrogenase isoform 1 (ALDH1) activity was assayed using ALDEFLUOR kit according to the ... and immediately analyzed by flow cytometry using 488 nm/561 nm lasers of FACS ARIA II. First, we obtained a PKH26(+) population ... Visualization and a three dimensional reconstruction of images were performed using a Zeiss LSM 510 confocal imaging system. N ... Soft agar assays. Assays were performed in 6-well cluster tissue culture plates pre-coated with 0.5% basal agar in DMEM:F12. ...
... and the demonstration of high-resolution multi-parametric imaging flow cytometry at unprecedented throughputs (in excess of ... Although the above methods are efficient at assaying small sample volumes on short timescales, they are typically inefficient ... Notable examples include the precision screening of over 1500 discrete enzymatic assays within 10 seconds ☍, ... Most recently, we have developed entirely new ways of imaging rapidly moving species within microfluidic flows. These methods ...
... including flow cytometry, mass spectrometry, high-content imaging and biophysical techniques. These direct and often label-free ... an orthogonal AMP-Glo assay and assays to identify redox cycling compounds. ... This cascade of assays and analysis resulted in the identification of three hit series. Chemical re‑synthesis confirmed one ... An insect cell line stably infected with Wolbachia was used by the A·WOL consortium to develop a whole cell assay which, in ...
Cell Based Assays. *Flow Cytometry. *Microscopes and Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes and Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes and Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes and Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
ELISAs or other plate reader-based assays. *Flow cytometry-based assays. *Western blot-based assays ... With PhenoVista, you get access to a world-class team with an unmatched combination of imaging expertise, assay development ... We know the choice of a partner to develop and implement imaging-based assays is mission critical to your success. ... Imaging. Automated microscopy including the latest confocal systems with integrated image analysis provides the backbone to our ...
Metabolism and endocrinology multiplex assays assist in the study of obesity, diabetes and associated conditions under the ... Publishing Revolutionary Research Imaging flow cytometry makes it possible. *Chromatography Sample Preparation Maintain clean ... Metabolism Biomarker Assay Kits Using Luminex® Technology. Request Information. Related Resources: Analyte Quarterly , ... Bring your metabolism biomarkers to life with MILLIPLEX® MAP Multiplex Assays. Bead-based multiplex assays enable precise, ...
Flow Cytometry. For infectivity assays, cells were infected with Ad CMV-GFP (MOI 5 and 50), typsinised 24 h pi, washed twice in ... Cell viability was assayed by MTT assay using a Victor3 plate reader (Perkin Elmer, Beaconsfield, UK). All viability assays ... Immunoblot images were scanned and band density of defined regions of interest in inverted jpg images was measured using ImageJ ... Cell cycle analysis of uninfected, asynchronous cells by propidium iodide flow cytometry demonstrated a strong correlation ...
Flow cytometry. Request a detailed protocol Eight to ten-week-old male and female Lrrc8a KO and WT littermate controls were ... CytoTox 96 Non-Radioactive Cytotoxicity (LDH) Assay. Promega. G1780. Commercial assay or kit. Mouse IL-1β/IL-1F2 DuoSet ELISA. ... Inflammasome activation assays. Request a detailed protocol Primary BMDMs were primed with LPS (1 µg mL−1, 4 hr) in DMEM (10% v ... Point visiting was used to allow multiple positions to be imaged within the same time-course and cells were maintained at 37°C ...
Cell Based Assays. *Flow Cytometry. *Microscopes and Cellular Imaging. *View All Cell Analysis ... Assay Percent Range. 96% min. (GC). MDL Number. MFCD00005508. Synonym. n-methylmaleimide, 1-methyl-1h-pyrrole-2,5-dione, ... Custom Assays, Antibodies, Oligos. Custom Assays, Antibodies, Oligos *Custom Assays, Antibodies, Oligos ...
Cell Based Assays. *Flow Cytometry. *Microscopes and Cellular Imaging. *View All Cell Analysis ... Assay Percent Range. 98%. Linear Formula. H2NNHCO(CH2)4CONHNH2. ... Custom Assays, Antibodies, Oligos *Custom Assays, Antibodies, ...
Cell Based Assays. *Flow Cytometry. *Microscopes and Cellular Imaging. *View All Cell Analysis ... Assay Percent Range. 97.5% min. MDL Number. MFCD00011634. Synonym. tetrabutylammonium tetrafluoroborate, tetra-n-butylammonium ... Custom Assays, Antibodies, Oligos. Custom Assays, Antibodies, Oligos *Custom Assays, Antibodies, Oligos ...
Cell Based Assays. *Flow Cytometry. *Microscopes and Cellular Imaging. *View All Cell Analysis ... Assay Percent Range. 98.5% min. (Argentometry). Linear Formula. (CH3)2CHCH2CH(NH2)CONH2·HCl. ... Custom Assays, Antibodies, Oligos. Custom Assays, Antibodies, Oligos *Custom Assays, Antibodies, Oligos ...
Cell Based Assays. *Flow Cytometry. *Microscopes. *Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes. *Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes. *Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes. *Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes. *Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes. *Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes. *Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes. *Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes. *Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ...
Cell Based Assays. *Flow Cytometry. *Microscopes. *Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ... Combine the trinocular optics with a SeBaCam camera for imaging and documentation ...
Cell Based Assays. *Flow Cytometry. *Microscopes. *Cellular Imaging. *View All Cell Analysis ... Applied Biosystems™ TaqMan® Assay and Arrays Search Tool. *Applied Biosystems™ TaqMan® Custom Assay Design Tools ... All excess blood flows into a closed, separate reservoir compartment automatically. *Includes a stoppered, pre-filled vial ...
  • North America is expected to account for the largest share of the global apoptosis assays market in 2018. (marketresearch.com)
  • It aims at estimating the market size and future growth potential of the global apoptosis assays market for different segments such as products, detection technology, applications, end users, and regions. (marketresearch.com)
  • The report reviews, analyzes and projects the global apoptosis assays market for the period 2017-2026 in terms of market value in US$ and the compound annual growth rates (CAGRs) projected from 2019 through 2024. (giiresearch.com)
  • Global Apoptosis Assays Market: Industry Status, Sales Ou. (pharmiweb.com)
  • The global apoptosis assays market revenue is expected to expand at a lucrative CAGR over the forecast period. (pharmiweb.com)
  • The global apoptosis assays market has been segmented on the basis of product type, detection technology, application, end user, and region. (pharmiweb.com)
  • On the basis of region, the global apoptosis assays market is segmented into North America, Europe, Asia Pacific, Latin America, and Middle East & Africa. (pharmiweb.com)
  • NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) ab112149 is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential. (abcam.com)
  • ab112149 is optimized for screening apoptosis activators and inhibitors with a flow cytometer. (abcam.com)
  • On the basis of detection technology, the apoptosis assays market is segmented into flow cytometry, cell imaging & microscopy, spectrophotometry, and other detection technologies. (marketresearch.com)
  • On the basis of application, the apoptosis assays market is segmented into drug discovery & development, basic research, stem cell research, and clinical & diagnostic application. (marketresearch.com)
  • Drug Discovery & Development is the principal application for Apoptosis Assays, which, however, is anticipated to maintain the slowest CAGR over the analysis period. (giiresearch.com)
  • Further growth in the market can be expected from government initiatives aimed at increasing the number of cell-based research activities, all of which point to a market for Apoptosis Assays that is flourishing. (giiresearch.com)
  • While COVID-19 may not have an adverse impact on the market for Apoptosis Assays as anticipated, there is a definite slowdown in demand, which has to be addressed with requisite measures. (giiresearch.com)
  • Accounting for an estimated share of 42.5% equating to US$1.7 billion in 2019, Apoptosis Assay Kits lead the demand for Apoptosis Assays globally, which is expected to maintain a 2019-2026 CAGR of 9.9% and reach a projected US$3.6 billion by 2026. (giiresearch.com)
  • The overall Apoptosis Assays market, globally, expected to touch US$4.5 billion in 2020. (giiresearch.com)
  • A major advantage of NucView® Caspase-3 Substrates compared to other apoptosis assays is that it can be used to monitor capase-3 activity in real time. (biotium.com)
  • Apoptosis assays is a programmed cell death that eliminates the old cells, unnecessary cells, and unhealthy cells without releasing harmful substances into the surrounding area. (pharmiweb.com)
  • However, dearth of skilled personnel for research and development of apoptosis assays may hamper growth of the target market over the forecast period. (pharmiweb.com)
  • North America apoptosis assays market accounts for highest revenue share in the target market and expected to dominate its presence over the forecast period. (pharmiweb.com)
  • The in vitro micronucleus assay is a well-established method for evaluating genotoxicity and cytotoxicity but scoring the assay using manual microscopy is laborious and suffers from subjectivity and inter-scorer variability. (jove.com)
  • The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry. (biotium.com)
  • Fluorescence produced by these fluorescent glucose analogs is proportional to the glucose uptake by the cells, and can be used to measure glucose uptake using flow cytometry and fluorescence microscopy. (news-medical.net)
  • On the basis of product type, the target market has been segmented into assay kits, reagents, microplates, and instruments. (pharmiweb.com)
  • Cell proliferation assays are performed using standard methods, including enzyme-linked immunosorbent assay (ELISA), flow cytometry, immunofluorescence and high content imaging. (cellsignal.com)
  • While the 3H-thymidine incorporation assay requires the use of radioactivity, BrdU incorporation is detected using an anti-BrdU antibody in ELISA, flow cytometry, or IF-IC applications and does not require the use of radioisotope labeling. (cellsignal.com)
  • Imaging flow cytometry (IFC), which combines the statistical power of traditional flow cytometry with the sensitivity and specificity of microscopy, has been recently used to perform the CBMN assay. (bioone.org)
  • To ensure reliable enumeration results, the EPCs are enriched during the procedure to increase the sensitivity of the flow cytometric analysis. (miltenyibiotec.com)
  • The quantitative application of HLA-based cellular microarrays, however, remains elusive in large part due to the large variability in the molecular nature of proteins, exacerbated by the lack of control over many protein-specific physical, chemical, and biological processes associated with microarray fabrication and with the sensitivity of the binding assay. (pubmedcentralcanada.ca)
  • With Amnis® charge-coupled device, time-delayed integration (CCD-TDI) technology , the CellStream® provides unparalleled high sensitivity in a highly customizable flow cytometer. (luminexcorp.com)
  • Cell quantitation and analysis techniques, such as hemocytometry, that are based on image analysis have limited sensitivity and objectivity, and sample sizes may be too small for statistical analysis. (luminexcorp.com)
  • Typically, cell viability assays provide a readout of cell health through measurement of metabolic activity, ATP content, or cell proliferation. (cellsignal.com)
  • Cell viability can also be assessed using cell toxicity assays that provide a readout on markers of cell death, such as a loss of membrane integrity. (cellsignal.com)
  • Thus, cell proliferation assays are a useful tool for assessing cell viability or cell survival by providing a readout on the number of actively dividing cells present in a sample. (cellsignal.com)
  • Cells stained with NIR Dye can be visualized with a flow cytometer at red excitation and far red emission (FL4 channel). (abcam.com)
  • See the Invitrogen Attune Acoustic Focusing Cytometer and find antibodies, antibody conjugates, and assay kits. (thermofisher.com)
  • The red label allows for direct detection of activated caspases in apoptotic cells by a flow cytometer. (abcam.com)
  • Using the ISX Mark II imaging flow cytometer, images of micronucleated mono- and binucleated cells as well as polynucleated cells can be captured at a high flow rate and automatically identified and scored in the Image Data Exploration and Analysis Software (IDEAS®) that accompanies the ISX. (merckmillipore.com)
  • The FlowSight® and ImageStream® Imaging Flow Cytometers add quantitative, microscopy imaging of every cell to your flow cytometry. (luminexcorp.com)
  • The Muse Cell Analyzer uses miniaturized fluorescence detection and microcapillary cytometry to deliver single-cell analysis, which is highly quantitative - especially when compared with traditional bulk methods like microscopy and Western blot. (luminexcorp.com)
  • As a result, Muse provides more quantitative results than non-flow based imaging systems, which only examine up to two parameters, are time-consuming, and provide less quantitative data. (luminexcorp.com)
  • Merck's FlowCellect™ GFP-LC3 Reporter Autophagy Assay Kits provide a quantitative solution for studying autophagy and measuring potency of autophagy inducers using flow cytometry. (merckmillipore.com)
  • This technique enables quantitative image analysis of the intracellular localization of the signaling intermediaries NF- κ B and NFAT as parameters of immune activity. (merckmillipore.com)
  • They are fully optimized for "plug-and-play" cellular analysis on Guava easyCyte™ and other flow cytometry instruments. (merckmillipore.com)
  • The ToxTracker reporter cell lines are combined in 96-well plates, exposed to the test samples, typically for 24h, and induction of the fluorescent reporters is examined by flow cytometry (Merck Guava® easyCyte). (merckmillipore.com)
  • The assay is typically performed using microscopy, which is labor intensive, time consuming and impractical after a large-scale radiological/nuclear event. (bioone.org)
  • The in vitro micronucleus (MN) assay is often used to evaluate cytotoxicity and genotoxicity but scoring the assay via manual microscopy is laborious and introduces uncertainty in results due to variability between scorers. (jove.com)
  • To remedy this, automated slide-scanning microscopy as well as conventional flow cytometry methods have been introduced in an attempt to remove scorer bias and improve throughput. (jove.com)
  • Eliminate the complexities of microscopy without compromising performance-Invitrogen EVOS Cell Imaging Systems. (thermofisher.com)
  • The cell imaging & microscopy segment is expected to register the highest CAGR during the forecast period. (marketresearch.com)
  • The increasing adoption of cell imaging and microscopy in diagnostic laboratories, drug discovery applications, and basic research is the major factor driving the growth of this market segment. (marketresearch.com)
  • The ES cells can be differentiated into neuronal precursor cells (NPCs) and ultimately striatal neurons that are tested using a panel of antibody markers and/or branched DNA assays. (criver.com)
  • Are NucView® Caspase-3 Substrates compatible with flow cytometry or microplate readers? (biotium.com)
  • In conclusion, a functional assay evaluating antibodies targeting PD-1 inhibition in vitro revealed that pembrolizumab is a slightly more effective PD-1 blocker than nivolumab, and that PD-L1 antibodies are superior to PD-1 antibodies in reverting PD-1 signaling. (nature.com)
  • At Charles River, we have developed robust and reproducible in vitro models that are validated for multiple assay platforms and are reliable tools to screen compounds. (criver.com)
  • In this webinar, adaptation of the in vitro MN assay to an imaging flow cytometry-based method will be described. (merckmillipore.com)
  • This work is the first demonstration of fully automated method for performing the in vitro MN assay on an imaging flow cytometry platform. (merckmillipore.com)
  • The integrative approach of the ToxTracker assay provides a powerful tool for in vitro cancer hazard screening of chemicals by unveiling activation of specific cellular signalling pathways upon exposure and deliver insight into the underlying mechanism of toxicity. (merckmillipore.com)
  • Assay development and optimization studies were designed to increase the yield of binucleated cells (BNCs), and improve data acquisition and analysis templates to increase the speed and accuracy of image analysis. (bioone.org)
  • Cells were analyzed by flow cytometry. (miltenyibiotec.com)
  • Cells were stained with CD34-FITC and CD133/2 (293C3)-PE and analyzed by flow cytometry. (miltenyibiotec.com)
  • In the past, highly trained lab technicians conducted assays manually, using a microscope to inspect thousands of individual cells to identify the biomarkers of DNA damage: the formation of micronuclei (MN) upon cell division (Figure 1). (mathworks.com)
  • My collaborator Dr. George Johnson's lab uses an IFC to collect multichannel image data from individual cells. (mathworks.com)
  • Once we have a normalized, clean set of data from the IFC, we use the trained network to classify images as having mono-, bi-, tri-, or tetra-nucleated cells with or without MN. (mathworks.com)
  • Discover the ideal assays to investigate your cells' health and behavior using our application-based selection tools. (thermofisher.com)
  • In addition, we used flow cytometry-based binding assays on the cells in our reporter system to explore whether it can be used to predict the functional potency of blocking antibodies. (nature.com)
  • There are numerous groups working on improving the situation for assays of senescent cells, but the academic researchers are largely aiming at something other than improvements that are convenient for human self-experimentation or later clinical tests. (fightaging.org)
  • Glucose uptake was evaluated in HeLa cells using BioVision's GluTracker™ Assay Kit (A) or BioVision's AGTracker™ Assay Kit (B). Cells were treated with or without 1X phloretin for 45 minutes. (news-medical.net)
  • Assays performed in commercially available cell lines typically do not result in clinically relevant data, but this challenge is addressed by using human ES cells and iPS-derived neurons from patient samples. (criver.com)
  • Cell viability assays are used to determine the overall health of cells, optimize culture or experimental conditions, and to measure cell survival following treatment with compounds, such as during a drug screen. (cellsignal.com)
  • Cell proliferation assays can measure cell division events directly (counting cells) or use markers of cell division/DNA replication such as DNA content. (cellsignal.com)
  • Cell proliferation assays typically measure DNA content or DNA synthesis in replicating cells. (cellsignal.com)
  • Cell cycle assays are used to determine the proportion of cells at different stages of the cell cycle via flow cytometry. (cellsignal.com)
  • For the cell cycle assay, cells are fixed or permeabilized, incubated with a DNA intercalating agent such as propidium iodide (PI) and subsequently analyzed by flow cytometry. (cellsignal.com)
  • Cell cycle distribution, cell viability, and apoptotic response were measured in CRY2 knockdown ( CRY2 -) and normal ( CRY2 +) cell populations using flow cytometry in cells with and without exposure to a mutagen challenge. (biomedcentral.com)
  • The comet assay results indicated that upon exposure to the same dose of chemical mutagen, CRY2 - cells accumulate significantly more unrepaired DNA damage than CRY2 + cells (P = 0.040), suggesting that CRY2 may be important for DNA repair. (biomedcentral.com)
  • Its microcapillary flow cell is engineered for acquisition of both suspension and adherent cells 2-60 microns in diameter. (luminexcorp.com)
  • Muse assays use relative fluorescence and flow cytometry to assess thousands of cells per sample, rendering data that is more accurate, more precise, and more reliable than traditional methods. (luminexcorp.com)
  • The dye is commonly used to selectively stain dead cells in a mixed live-dead population and also used as a counterstain in multicolor fluorescent assays. (biotium.com)
  • Imaging flow cytometry (IFC) has emerged as a useful and efficient tool for studying the signaling pathways in immunophenotypically defined subpopulations of immune cells. (merckmillipore.com)
  • Luminex flow cytometers and cellular analysis instruments give you instant access to all facets of cellular phenotypes and morphology. (luminexcorp.com)
  • However, these values are not available for therapeutic PD-1 and PD-L1 antibodies, and the ability of these agents to block PD-1 signaling have not been compared in a functional assay. (nature.com)
  • This is essentially why I chose to support the work of CellAge , given their focus on clinically useful assays of cellular senescence. (fightaging.org)
  • Phenotypic assays using various neuronal cell models are robust tools to discover new disease targets, perform HT screens, understand mechanism of action, and develop clinically relevant biomarkers. (criver.com)
  • Some users have reported performing time course assays with NucView® 488 Caspase-3 Substrate for 4-5 days. (biotium.com)
  • NucView® Caspase-3 Substrates are compatible with flow cytometers and other instruments with the appropriate excitation and emission settings. (biotium.com)
  • Together, cell viability and cell toxicity assays are important tools for assessing cellular responses to experimental compounds of interest. (cellsignal.com)
  • The Muse Cell Analyzer packs 3-parameter analysis into a compact, easy to use benchtop device, making flow cytometry accessible to anyone, anytime. (luminexcorp.com)
  • The Muse Instrument has an integrated touchscreen and software for data acquisition and analysis using optimized Muse Assays. (luminexcorp.com)
  • Muse assays are available for precision cell counts, as well as single-cell measurement of critical cell parameters. (luminexcorp.com)
  • Muse features a highly intuitive touchscreen interface that allows simple step-by-step operation, so easy that no flow expertise is required to run assays. (luminexcorp.com)
  • The figure below shows the data output for the Muse Count & Viability Assay. (luminexcorp.com)
  • Serve as a hands-on technician performing molecular biology assays, including DNA isolation, DNA sequencing, PCR amplification, protein purification and Western blots. (monster.com)
  • We present a novel cellular microarray assay using soluble peptide-loaded HLA A2-Ig dimer complexes that optimizes the avidity of peptide-HLA binding by preserving the molecular flexibility of the dimer complex, while attaining much higher concentrations of the complex relative to cognate T-cell receptors. (pubmedcentralcanada.ca)
  • A seminal advance in assay development is made by separating the molecular T-cell receptor recognition event from the binding interactions that lead to antigen-specific cell capture on the microarray. (pubmedcentralcanada.ca)
  • EGFR molecular imaging provides non-invasive whole-body images capable of detecting primary tumors and metastases, which can be used to diagnose and monitor response to therapy. (thno.org)
  • We evaluated properties of two anti-EGFR fragments, 8708 and 8709, as molecular-targeted imaging probes. (thno.org)
  • Molecular-targeted imaging probes are used to identify tumors, define tumor markers and margins, and monitor tumor response to therapy. (thno.org)
  • DNA damage accumulation was measured using the single cell gel electrophoresis (comet) assay, and damage was quantified using the Olive tail moment, which considers the amount and distance of DNA migration away from the nucleus, indicative of DNA strand breaks. (biomedcentral.com)
  • We use DeepFlow, a deep learning network optimized for use with IFC [1], which enables us to accurately and automatically categorize the images as single nucleus, single nucleus with MN, two nuclei, or two nuclei with MN (Figure 2). (mathworks.com)
  • This approach eliminates the subjectivity of manual approaches and enables assays to be performed with consistent results in labs around the world. (mathworks.com)
  • The assay enables the use of cellular microarrays to determine the stability and flux of antigen-specific T-cell responses within and across populations. (pubmedcentralcanada.ca)
  • Abcam Activity Assay kits are a set of tools for monitoring cellular functions. (abcam.com)
  • The assay kits segment is expected to hold major share in terms of revenue in the target market over the forecast period. (pharmiweb.com)
  • The CHO reporter cell line is ideal for flow cytometry applications, while U20S is suitable for both imaging and flow cytometry. (merckmillipore.com)
  • ToxTracker is a mammalian stem cell-based reporter assay that detects activation of specific cellular signalling pathways upon exposure to unknown compounds (Hendriks et al, Tox Sci 2016). (merckmillipore.com)
  • The user interface is designed to be intuitive, so you spend less time with experimental setup and analysis for the critical cell health assays you need most. (luminexcorp.com)
  • Listen and watch our cell analysis webinars to learn about the latest technologies, experimental tips and troubleshooting strategies for simple cell counts, multidimensional cell analysis, imaging flow cytometry and more. (merckmillipore.com)
  • A user-friendly touchscreen interface, intuitive cell analysis software, and optimized assays work to simplify your research. (luminexcorp.com)
  • Insights of the mechanism of competitive endogenous RNAs (ceRNAs) were obtained from bioinformatic analysis, luciferase assays and RIP assays. (nih.gov)
  • The cell imaging and analysis system segment is projected to witness highest growth rate in the target market over the forecast period. (pharmiweb.com)
  • This paper describes the protocol developed to perform a fully automated version of the assay using multispectral imaging flow cytometry. (jove.com)
  • Genotoxicity assays are used to assess the damage to genetic material (DNA and RNA) caused by a chemical agent such as a drug, and are often used to test the safety of candidate drugs. (mathworks.com)
  • In contrast, the market for these assays in Stem Cell Research is poised to grow at a rapid pace, owing to the increasing number of stem cell research activities and an increasing investment in the same. (giiresearch.com)
  • We show that CIH followed by normoxia increases the bone marrow hypoxic area, increases the number of multipotent hematopoietic progenitors (CFU assay), promotes erythropoiesis, and increases monocyte counts. (springer.com)
  • BioVision's AGTracker™ (for 1,5-AG) and GluTracker™ (for Glucose) Uptake Assays are useful tools for drug discovery and identifying potential modulators in glucose-dependent pathways. (news-medical.net)
  • CXCR2 and G protein-coupled receptor kinase 2 expressions in neutrophils were measured by flow cytometry. (jimmunol.org)
  • Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to a variety of cell-based microassays. (osapublishing.org)