Osteopontin: A negatively-charged extracellular matrix protein that plays a role in the regulation of BONE metabolism and a variety of other biological functions. Cell signaling by osteopontin may occur through a cell adhesion sequence that recognizes INTEGRIN ALPHA-V BETA-3.Sialoglycoproteins: Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.Integrin-Binding Sialoprotein: A highly glycosylated and sulfated phosphoprotein that is found almost exclusively in mineralized connective tissues. It is an extracellular matrix protein that binds to hydroxyapatite through polyglutamic acid sequences and mediates cell attachment through an RGD sequence.Osteoblasts: Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.Calcinosis: Pathologic deposition of calcium salts in tissues.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Calcification, Physiologic: Process by which organic tissue becomes hardened by the physiologic deposit of calcium salts.Receptors, Vitronectin: Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.Osteocalcin: Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Alkaline Phosphatase: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 220.127.116.11.Osteogenesis: The process of bone formation. Histogenesis of bone including ossification.Melanoma: A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Prognosis: A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.Sentinel Lymph Node Biopsy: A diagnostic procedure used to determine whether LYMPHATIC METASTASIS has occurred. The sentinel lymph node is the first lymph node to receive drainage from a neoplasm.Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.Skin Neoplasms: Tumors or cancer of the SKIN.ArchivesBiological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Rodenticides: Substances used to destroy or inhibit the action of rats, mice, or other rodents.Rodent Control: The reduction or regulation of the population of noxious, destructive, or dangerous rodents through chemical, biological, or other means.Rodent Diseases: Diseases of rodents of the order RODENTIA. This term includes diseases of Sciuridae (squirrels), Geomyidae (gophers), Heteromyidae (pouched mice), Castoridae (beavers), Cricetidae (rats and mice), Muridae (Old World rats and mice), Erethizontidae (porcupines), and Caviidae (guinea pigs).4-Hydroxycoumarins: Substances found in many plants, containing the 4-hydroxycoumarin radical. They interfere with vitamin K and the blood clotting mechanism, are tightly protein-bound, inhibit mitochondrial and microsomal enzymes, and are used as oral anticoagulants.Durapatite: The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.Cell-Matrix Junctions: Specialized areas at the CELL MEMBRANE where a cell attaches to the EXTRACELLULAR MATRIX or other substratum.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)25-Hydroxyvitamin D3 1-alpha-Hydroxylase: A mitochondrial cytochrome P450 enzyme that catalyzes the 1-alpha-hydroxylation of 25-hydroxyvitamin D3 (also known as 25-hydroxycholecalciferol) in the presence of molecular oxygen and NADPH-FERRIHEMOPROTEIN REDUCTASE. This enzyme, encoded by CYP27B1 gene, converts 25-hydroxyvitamin D3 to 1-alpha,25-dihydroxyvitamin D3 which is the active form of VITAMIN D in regulating bone growth and calcium metabolism. This enzyme is also active on plant 25-hydroxyvitamin D2 (ergocalciferol).Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.Thermodilution: Measurement of blood flow based on induction at one point of the circulation of a known change in the intravascular heat content of flowing blood and detection of the resultant change in temperature at a point downstream.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Digoxin: A cardiotonic glycoside obtained mainly from Digitalis lanata; it consists of three sugars and the aglycone DIGOXIGENIN. Digoxin has positive inotropic and negative chronotropic activity. It is used to control ventricular rate in ATRIAL FIBRILLATION and in the management of congestive heart failure with atrial fibrillation. Its use in congestive heart failure and sinus rhythm is less certain. The margin between toxic and therapeutic doses is small. (From Martindale, The Extra Pharmacopoeia, 30th ed, p666)Hematoma, Subdural, Intracranial: Accumulation of blood in the SUBDURAL SPACE over the CEREBRAL HEMISPHERE.Newspapers: Publications printed and distributed daily, weekly, or at some other regular and usually short interval, containing news, articles of opinion (as editorials and letters), features, advertising, and announcements of current interest. (Webster's 3d ed)Journalism, Medical: The collection, writing, and editing of current interest material on topics related to biomedicine for presentation through the mass media, including newspapers, magazines, radio, or television, usually for a public audience such as health care consumers.Research Report: Detailed account or statement or formal record of data resulting from empirical inquiry.Echocardiography: Ultrasonic recording of the size, motion, and composition of the heart and surrounding tissues. The standard approach is transthoracic.MassachusettsFreezing: Liquids transforming into solids by the removal of heat.Hypertrophy, Left Ventricular: Enlargement of the LEFT VENTRICLE of the heart. This increase in ventricular mass is attributed to sustained abnormal pressure or volume loads and is a contributor to cardiovascular morbidity and mortality.Adult Children: Children who have reached maturity or the legal age of majority.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Glycosylation: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.Integrins: A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.N-Acetylgalactosaminyltransferases: Enzymes that catalyze the transfer of N-acetylgalactosamine from a nucleoside diphosphate N-acetylgalactosamine to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.Selenoprotein P: An extracellular selenoprotein that contains most of the SELENIUM in PLASMA. Selenoprotein P functions as an antioxidant and appears to transport selenium from the LIVER to peripheral tissues.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
... and osteopontin as protein biomarkers for malignant mesothelioma: analytical validation of ELISA based assays and ... Feb 2010). "Reduction of elevated plasma osteopontin levels with resection of non-small cell lung cancer". J Clin Oncol. 28 (6 ... Blasberg, JD; Pass, HI; Goparaju, CM; Flores, RM; Lee, S; Donington, JS (Feb 2010). "Reduction of elevated plasma osteopontin ...
"The sensitive RT-PCR assays, in addition to the immunological and histochemical data, showed that neither the adipogenic nor ... but they did express osteopontin and alkaline phosphatase". In a controlled manner, isolated and expanded human mesenchymal ... and the expanded mesenchymal cells from at least 50 donors were tested in three lineage assays, with all tested donors ... responding positively in these assays. The cells did not differentiate spontaneously during culture expansion. Similarly ...
... osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. It was reported that ERRα ... response elements as a result of transient transfection assays, and a possible role of ERRα in steroidogenesis with relation to ...
Elevations of serum mesothelin specific to ovarian and other cancer patients may be measured using ELISA assays. Assays for ... Creaney J, Yeoman D, Demelker Y, Segal A, Musk AW, Skates SJ, Robinson BW (August 2008). "Comparison of osteopontin, ... depending on the assay used and thus that normal levels must be determined anew when new assays are introduced. GRCh38: Ensembl ... "Sensitive and specific new enzyme-linked immunosorbent assay for N-ERC/mesothelin increases its potential as a useful serum ...
Zohar R, Suzuki N, Suzuki K, Arora P, Glogauer M, McCulloch CA, Sodek J (Jul 2000). "Intracellular osteopontin is an integral ... Memory cell proliferation (activation) can also be assayed in vitro with CFSE chemical tagging. In addition, variations in CD44 ... CD44 is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix ... Fibronectin, FYN, Hyaluronan, Lck, Osteopontin Selectins, and Src. GRCh38: Ensembl release 89: ENSG00000026508 - Ensembl, May ...
An in vitro assay was accordingly developed to identify actives which prevent or reduce myofibroblast formation and thus ... Molecular mechanisms linking wound inflammation and fibrosis: knockdown of osteopontin leads to rapid repair and reduced ... Research has also implicated osteopontin in scarring. Sherratt, Jonathan A. (2010). "Mathematical Modelling of Scar Tissue ...
1992). "A new assay for beta-thromboglobulin in urine.". Thromb. Res. 64 (1): 33-43. PMID 1837963. doi:10.1016/0049-3848(91) ... Osteopontin ...
Integrin ligands (collagens, fibrinogen, fibronectin, laminins, ICAM-1, ICAM-2, osteopontin, VCAM-1, vitronectin) ... In adenylate cyclase activation assays, CRH-related peptides are 10 times more potent at stimulating CRHR2 beta than CRHR2 ...
Integrin ligands (collagens, fibrinogen, fibronectin, laminins, ICAM-1, ICAM-2, osteopontin, VCAM-1, vitronectin) ... either as an assay for their reactivity or to suppress damage to DNA, proteins, and polysaccharides. In analytical ...
Results: The SMRP assay demonstrates intra-assay CVs of 2.3% and 3% (at 4.6 nM and 13.7 nM, respectively), and inter-assay CVs ... The OPN assay demonstrates intra-assay CVs of 5.8%, 4.1%, and 5.2% (at 1.9, 5.1, and 11.1 ng/mL, respectively), and inter-assay ... Conclusions: These assays provide reliable and reproducible quantitation of SMRP and OPN proteins. Both are increased in ... Soluble mesothelin related peptides (SMRP) and osteopontin (OPN) have gained interest in recent years for this purpose. Methods ...
Gene Expression Profiling of Paraffin-Embedded Primary Melanoma Using the DASL Assay Identifies Increased Osteopontin ... Gene Expression Profiling of Paraffin-Embedded Primary Melanoma Using the DASL Assay Identifies Increased Osteopontin ... Gene Expression Profiling of Paraffin-Embedded Primary Melanoma Using the DASL Assay Identifies Increased Osteopontin ... Gene Expression Profiling of Paraffin-Embedded Primary Melanoma Using the DASL Assay Identifies Increased Osteopontin ...
Gene expression profiling of paraffin embedded primary melanoma using the DASL assay identifies increased osteopontin ... Co-expression of genes with osteopontin. The expression of genes most closely correlated with osteopontin expression was ... This inter-relation between Osteopontin and NFκβ complex is pictured on the Ingenuity network (Figure 1). Osteopontin also ... Osteopontin: role in cell signaling and cancer progression. Trends Cell Biol. 2006;16:79-87. [PubMed] ...
Assays. A1C was determined locally by standardized assays, and serum lipid and lipoprotein concentrations were analyzed ... Osteopontin mRNA expression in a chemically-induced model of atherogenesis. Ann N Y Acad Sci 1995;760:354-356pmid:7785915. ... Increased osteopontin levels in the vitreous of patients with diabetic retinopathy. Ophthalmic Res 2007;39:143-147pmid:17505146 ... The role of osteopontin in tumorigenesis and metastasis. Invasion Metastasis 1997;17:1-15pmid:9425320. ...
A cohort of 250 MG patients treated with standardized protocol of GCs were recruited, and plasma osteopontin (OPN) and ... We hypothesized the pro-inflammatory cytokine, osteopontin (OPN), may be associated with variability of response to ... We hypothesized the pro-inflammatory cytokine, osteopontin, may be associated with variability of response to glucocorticoids ( ... A sensitive non-isotopic assay for acetylcholine receptor autoantibodies. Clin Chim Acta (2006) 364(1-2):159-66. doi:10.1016/j. ...
View our 38 Osteopontin/OPN products for your research including Osteopontin/OPN Primary Antibodies, Proteins and Enzymes, ... Proteome Profiler Antibody Arrays, ELISAs, Luminex Assays, and Simple Plex Assays. ... Osteopontin/OPN: Products. Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that ... Simple Plex Mouse/Rat Osteopontin/OPN Cartridge Simple Plex Cartridge Kit for 72 samples, containing Osteopontin for use with ...
The clonogenicity was determined by a soft agar assay. B, soft agar clonogenicity assay of primary CD44+ colorectal cancer ... The potential of osteopontin as a therapeutic target for human colorectal cancer. J Gastrointest Surg 2011;15:652-9. ... Osteopontin induced by macrophages contribute to metachronous liver metastases in colorectal cancer. Am Surg 2011;77:1515-20. ... Role of osteopontin in adhesion, migration, cell survival and bone remodeling. Exp Oncol 2004;26:179-84. ...
The search for a BRMS1-regulated site on the OPN promoter, using luciferase reporter assays of the promoter deletions, ... Osteopontin (OPN), a secreted phosphoglycoprotein, has been strongly associated with tumor progression and aggressive cancers. ... Breast cancer metastasis suppressor 1 (BRMS1) inhibits osteopontin transcription by abrogating NF-kappaB activation.. Samant RS ... Breast cancer metastasis suppressor 1 (BRMS1) inhibits osteopontin transcription by abrogating NF-κB activation ...
Effects of osteopontin-enriched formula on lymphocyte subsets in the first 6 months of life: a randomized controlled trial.. ... BackgroundHuman milk is rich in osteopontin (OPN), which has immunomodulatory functions.MethodsIn a randomized controlled trial ...
cell adhesion Source: RGD ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a direct assay for the function, process or ... response to steroid hormone Source: RGD ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a direct assay for the function, ... cell projection Source: RGD ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a direct assay for the function, process or ... positive regulation of bone resorption Source: RGD ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a direct assay for the ...
ELISA kit for human Osteopontin/OPN (Cat#SOST00). 0.024 ng/mL detection sensitivity. View Osteopontin/OPN ELISA kit details. ... Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess ... The recovery of Osteopontin spiked to three different levels throughout the range of the assay in various matrices was ... Assay Procedure. Refer to the product for complete assay procedure.. Bring all reagents and samples to room temperature before ...
A) Cellular proliferation assay (BrdU incorporation). Mouse cholangiocyte cell line 603B and primary human cholangiocytes were ... The osteopontin level in liver, adipose tissue and serum is correlated with fibrosis in patients with alcoholic liver disease ... Osteopontin is up-regulated in chronic hepatitis C and is associated with cellular permissiveness for hepatitis C virus ... Osteopontin is up-regulated in chronic hepatitis C and is associated with cellular permissiveness for hepatitis C virus ...
Enzyme-Linked Immunosorbent Assay * Female * Follow-Up Studies * GPI-Linked Proteins / blood* ... Introduction: Soluble mesothelin (SM), megakaryocyte potentiating factor (MPF), and osteopontin (OPN) are blood biomarkers of ... Soluble Mesothelin, Megakaryocyte Potentiating Factor, and Osteopontin as Markers of Patient Response and Outcome in ...
The osteopontin transcript (SPP1) was identified in the somatic cells from cows experimentally infected with Escherichia coli. ... Further study by in vitro assay using reporter genes and binding shift assays could help elucidate the impact of promoter ... Osteopontin (or secreted phosphoprotein 1, SPP1) is a cytokine produced by macrophages and activated T cells . Osteopontin ... Osteopontin is also associated with cholesterol gallstone formation in human and mouse . The specific role of osteopontin ...
Detect osteopontin protein with anti-osteopontin antibody products and ELISA kits for osteopontin test methods. Learn more ... Measuring osteopontin protein using anti-osteopontin antibody products and ELISA as an osteopontin test. Osteopontin protein, ... Specific detection of osteopontin protein domains. Detection of specific osteopontin protein isoforms may help elucidate their ... Figure 1. Anti-osteopontin antibodies and ELISA kits for quantifying osteopontin protein.. ...
Plasma levels of OPN were measured using the enzyme-linked immunosorbent assay (ELISA) kit Quantikine Human Osteopontin ... Association of plasma osteopontin with diabetic retinopathy in Asians with type 2 diabetes. Xiao Zhang,1 Wai Kitt Chee,2 Sylvia ... Purpose: Osteopontin (OPN) is a proinflammatory cytokine with diverse functions. Increased levels of OPN in vitreous fluid have ... FinnDiane Study G. Osteopontin is a strong predictor of incipient diabetic nephropathy, cardiovascular disease, and all-cause ...
Plasma OPN levels were measured by ELISA (Human OPN assay kit; IBL), which measures total concentration of phosphorylated and ... Potential roles of osteopontin and αvβ3 integrin in the development of coronary artery restenosis after angioplasty. Proc Natl ... High levels of osteopontin (OPN) mRNA and proteins were reported in atherosclerotic plaques.1,2 Recently, we reported plasma ... Osteopontin is elevated during neointima formation in rat arteries and is a novel component of human atherosclerotic plaques. J ...
Shop a large selection of products and learn more about Thermo Scientific Lab Vision Osteopontin, Rabbit Polyclonal Antibody:: ... RNAi, Oligos, and Assays Tools RNAi, Oligos,and Assays Tools Oligos Tools. ... Immunogen: A synthetic peptide derived from internal domain of human Osteopontin. Molecular Weight: 34kDa. Positive Control: ... Osteopontin is a 34kDa extracellular matrix protein with a cell-binding domain. It was originally identified as a major ...
Genotyping Assay Market to reflect impressive growth in Biotechnology Industry 2020-08-09 ... What insights does the Osteopontin market report provide to the readers?. *Osteopontin market fragmentation on the basis of ... Questionnaire answered in the Osteopontin market report include:. *How the market for Osteopontin has grown over the historic ... Osteopontin Market Size Estimated to Observe Significant Growth by 2028. Posted on 2019-12-16 by Mark Hub in Food & Beverage ...
... request pricing from manufacturers of cell adhesion assays, including extracellular matrix & angiogenesis assays ... Corning® Osteopontin, Human, 50µg. Corning Life Sciences. Source Osteopontin - Human breast milk Quality Filtered (0.2 µm ... Cell Adhesion Assays Cell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for ... Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews ...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow ... Anti-Osteopontin antibody produced in goat affinity isolated antibody, lyophilized powder Synonym: Anti-OPN ... purified recombinant mouse osteopontin expressed in NSO cells. Physical form Lyophilized from a 0.2 μm filtered solution in ... The OPN (osteopontin) gene is mapped to mouse chromosome 5 E5 and codes for secreted phosphoprotein 1 and is mostly expressed ...
Plasma osteopontin was measured in duplicate by using an enzyme-linked immunosorbent assay (Calbiochem, Inc) with an intra- ... Recent investigations have highlighted the importance of the matricellular protein osteopontin as a key mediator in the ... Fasting blood samples were drawn and frozen at −70°C without any freeze-thaw cycles until assay. ... Accordingly, we investigated the clinical and echocardiographic correlates of plasma osteopontin in a community-based sample. ...
Furthermore, in a migration assay osteopontin had the capacity to induce directed migration of astrocytes.18 ... Osteopontin mRNA colocalized with a microglia marker, ED1 (Figure 3A⇓, arrow); osteopontin mRNA (Figure 3B⇓, arrow) did not ... Results-Osteopontin mRNA was expressed de novo in the peri-infarct region from 3 to 48 hours; by 5 days osteopontin mRNA ... Osteopontin mRNA is expressed very early after ischemia in the peri-infarct region. The timing of osteopontin gene expression ...
Cell proliferation was assayed by examining uptake of bromodeoxyuridine (BrdU). ... S. Tanaka, S. Saika, S. Kon, T. Uede, W. Kao, S. R. Rittling, D. T. Denhardt, Y. Ohnishi; Loss of Osteopontin Perturbs the ... Purpose:: To investigate the role of osteopontin (OPN) in the wound healing process of the lens epithelium. We previously ... Loss of Osteopontin Perturbs the Epithelial-Mesenchymal Transition in an Injured Mouse Lens Epithelium ...
Here we report that osteopontin (OPN), a cytokine involved in homeostatic and pathophysiologic responses, is constitutively ... Here we report that osteopontin (OPN), a cytokine involved in homeostatic and pathophysiologic responses, is constitutively ... Supernatants were harvested after 48 h and assayed by ELISA for CCL5 (left) and OPN (right) (mean ± SEM of four independent ... Osteopontin: a key cytokine in cell-mediated and granulomatous inflammation. Int J Exp Pathol (2000) 81(6):373-90. doi:10.1046/ ...
Enzyme-LinkeHuman OsteopontinAntibodySPP1Levels of osteopontinExpression of osteopontinGeneAntibodiesPlasmaConcentrationsExtracellular MatrixProtein isoformsKnown as bone sialoproteinProteinsIntegrinAlkaline phosphataseCytokineIntra-assayPromoterELISA KitRole of OsteopontinLigandBiomarkersFibronectinArbor AssaysTumorCollagenSecretionInter-assayOffered as premixedChromatinLuciferaseSerumSolubleRecombinantUrineMarkersMRNA expressionGlycoproteinProliferationGenesImmunoassayDiscerning preclinical and clinicalDetectionBead-basedPhosphorylationConcentrationReporterBiomarker
- Plasma levels of OPN were measured using enzyme-linked immunosorbent assay. (molvis.org)
- Tear levels of cytokines, interleukin-2(IL-2), interferon gamma (IFN-γ), IL-4, IL-5, IL-6, IL-8 and IL-13, in 49 patients with ACD (allergic conjunctivitis 14 cases, atopic keratoconjunctivitis 14 cases and vernal keratoconjunctivitis 21 cases) were measured with ELISA (enzyme-linked immunosorbent assay) method. (nii.ac.jp)
- The enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were respectively used to measure the plasma osteopontin level and its gene polymorphisms. (elsevier.com)
- New inhibitors using brefelamide, an aromatic amide isolated from Dictyostelium cellular slim molds which may inhibit OPN transcription at concentration of 1M, were synthesized as compounds C, D and E. Their inhibitory activity against OPN synthesis in phorbol 12-myristate 13-acetate (PMA)-stimulated THP-1 cells was confirmed using enzyme-linked immunosorbent assay (ELISA), a multicolor immune-fluorescent microscope and western blot analysis. (preprints.org)
- The Quantikine Human Osteopontin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteopontin in cell culture supernates, plasma, urine, and human milk. (rndsystems.com)
- It contains NS0-expressed recombinant human Osteopontin and has been shown to accurately quantitate the recombinant factor. (rndsystems.com)
- Results obtained using natural human Osteopontin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. (rndsystems.com)
- These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteopontin. (rndsystems.com)
- This motif (amino acid sequence SVVYGLR in human osteopontin) has been postulated to be involved in neutrophil erosion of inflammatory cells and neutrophilic migration. (clontech.com)
- Osteopontin antibody LS-C212311 is an unconjugated mouse monoclonal antibody to human Osteopontin (SPP1). (lsbio.com)
- We have generated synthetic peptides corresponding to various portions of human osteopontin (OPN) and have immunized rabbits and mice with these peptides to generate polyclonal and monoclonal antibodies specific to human OPN. (elsevier.com)
- Recombinant corresponding to full-length human Osteopontin. (lsbio.com)
- Human Osteopontin at C-terminal (aa167-314). (lsbio.com)
- Toward this end, we offer a series of anti-osteopontin antibody products ( monoclonal , polyclonal , and directed toward N-terminal domains ), as well as osteopontin ELISA kits , that may be used in osteopontin test procedures for research studies. (clontech.com)
- A summary of anti-osteopontin antibody and osteopontin test ELISA kits is presented in the illustration below. (clontech.com)
- 5 Liaw et al 6 showed anti-osteopontin antibody treatment to reduce neointimal formation after injury in rat arteries. (ahajournals.org)
- Take advantage of a quality product that delivers accurate results with the Thermo Scientific Osteopontin, Rabbit Polyclonal Antibody. (fishersci.com)
- The α v β 3 integrin was most important for osteopontin-mediated NF-κB induction and survival, since adding a neutralizing anti-β 3 integrin antibody blocked NF-κB activity and induced endothelial cell death when cells were plated on osteopontin. (rupress.org)
- Chromatin immunoprecipitation assay using an antibody to NFATc1 also showed enrichment at the cyclin D1 promoter. (ahajournals.org)
- Singleplex R-PLEX assays are created on MSD GOLD Small Spot Streptavidin plates, while multiplex assays are designed by combining R-PLEX Antibody Sets on U-PLEX plates. (mesoscale.com)
- In the next step you will be given the option to add U-PLEX Antibody Sets to your multiplex assay. (mesoscale.com)
- The R-PLEX Human CD5 and CD20 Antibody Sets require a final concentration of 2% Triton X-100 in the assay diluent. (mesoscale.com)
- The gene whose expression was most differentially expressed in association with relapse free survival in study 1 was osteopontin ( SPP1 , p=2.11×10 −6 ) and supportive evidence for this was obtained in study 2 used as a validation set (p=0.006) (unadjusted data). (pubmedcentralcanada.ca)
- We hypothesized the pro-inflammatory cytokine, osteopontin (OPN), may be associated with variability of response to glucocorticoids (GCs) in patients with MG. A cohort of 250 MG patients treated with standardized protocol of GCs was recruited, and plasma OPN and polymorphisms of its gene, secreted phosphoprotein 1 ( SPP1 ), were evaluated. (frontiersin.org)
- The osteopontin transcript ( SPP1 ) was identified in the somatic cells from cows experimentally infected with Escherichia coli . (biomedcentral.com)
- High levels of osteopontin (OPN) mRNA and proteins were reported in atherosclerotic plaques. (ahajournals.org)
- RESULTS: HepG2 cells constitutively expressed low levels of osteopontin. (wikigenes.org)
- The present invention provides diagnostic methods based on the serum levels of osteopontin. (patentsencyclopedia.com)
- The present study was performed to determine the spatial and temporal expression of osteopontin and its integrin receptor α v β 3 during formation of the peri-infarct gliotic barrier and subsequent formation of a new glial-limiting membrane. (ahajournals.org)
- The spatial and temporal expression of osteopontin mRNA was determined by in situ hybridization, and that of osteopontin ligand and its integrin receptor α v β 3 was determined by immunohistochemistry. (ahajournals.org)
- Conclusions -Early microglial/macrophage expression of osteopontin mRNA defines the borders and final infarct area at 24 hours. (ahajournals.org)
- The OPN (osteopontin) gene is mapped to mouse chromosome 5 E5 and codes for secreted phosphoprotein 1 and is mostly expressed in bone tissue. (sigmaaldrich.com)
- Vitamin D (1,25 D3) is an important transcriptional regulator of many genes including our gene of interest, osteopontin. (wisconsin.edu)
- A luciferase assay normalized by a tl-gal assay was used to measure gene expression. (wisconsin.edu)
- Alternative splicing of the osteopontin (OPN) gene generates three protein splicing isoforms (OPN-SI), designated as OPNa, OPNb and OPNc. (aacrjournals.org)
- We report that persistent C. trachomatis infection increases the expression of inflammatory tumour cytokines and upregulates molecular biomarkers such as podoplanin, Wilms' tumour gene 1 and osteopontin in primary cultures of mesothelial cells (Mes1) and human mesothelioma cells (NCI). (microbiologyresearch.org)
- Finally, real-time PCR analysis revealed a strong induction of podoplanin, Wilms' tumour gene 1 and osteopontin gene expression in infected Mes1 cells. (microbiologyresearch.org)
- Levels of plasminogen-activator inhibitor 1 gene, vascular endothelial growth factor, and osteopontin are measured by immunoenzyme techniques. (knowcancer.com)
- Our purpose here was to detect the association among plasma osteopontin concentration, single nucleotide polymorphisms (SNPs) of osteopontin gene, and pelvic inflammatory disease (PID). (elsevier.com)
- A promoter polymorphism of the osteopontin (OPN) gene (rs28357094) has been associated with multiple inflammatory states, severity of Duchenne muscular dystrophy (DMD) and muscle size in healthy young adults. (cdc.gov)
- Anti-osteopontin antibodies and ELISA kits for quantifying osteopontin protein. (clontech.com)
- An adhesion assay using function-blocking antibodies against αvβ3 and β1 integrins, as well as αvβ3 integrin-overexpressing A549 cells, revealed that site-specific O-glycosylation affected the association of OPN with the two integrins. (portlandpress.com)
- We then generated six distinct sandwich enzyme-linked immunoabsorbent assay (ELISA) systems by using different pairs of polyclonal and monoclonal antibodies against human OPN. (elsevier.com)
- Simple Plex Cartridge Kit for 72 samples, containing Osteopontin for use with Mouse and Rat Plasma/Serum/Urine. (rndsystems.com)
- 1 To our knowledge, no prior study has investigated whether plasma osteopontin concentrations are related to cardiovascular disease risk factors, valve calcification, and LV dilatation and hypertrophy in a community-based sample. (bmj.com)
- Accordingly, we investigated the clinical and echocardiographic correlates of plasma osteopontin in a community-based sample. (bmj.com)
- Plasma osteopontin concentrations in preeclampsia - is there an association with endothelial injury? (degruyter.com)
- Here we show that blocking the adhesive function of αvβ3 integrin with soluble RGD ligands, such as osteopontin or cilengitide, promoted association of Rab-coupling protein (RCP) with α5β1 integrin and drove RCP-dependent recycling of α5β1 to the plasma membrane and its mobilization to dynamic ruffling protrusions at the cell front. (rupress.org)
- All patients were sampled twice before receiving treatment and the mean values of plasma protein concentration of the several CAF were used, in order to reduce the impact of intra-patient and assay variability. (esmo.org)
- Lack of sufficient plasma volume hindered homogeneous use of both assays for all samples. (esmo.org)
- The level of plasma osteopontin was elevated in patients with PID as compared to that of healthy women and decreased significantly after treatment. (elsevier.com)
- Plasma osteopontin concentration was significantly correlated with white blood cell (WBC) and neutrophil counts and plasma C-reactive protein (CRP) level in patients with PID. (elsevier.com)
- Plasma osteopontin concentration was not associated with osteopontin polymorphism. (elsevier.com)
- When the cutoff level of the plasma osteopontin concentration was set to be 58.53 ng/mL, the adjusted odds ratio of plasma osteopontin for PID risk was 3.87 (95% confident interval: 1.30-11.51). (elsevier.com)
- Plasma osteopontin level may act as a prediction marker for PID. (elsevier.com)
- Plasma osteopontin (OPN) levels are elevated in mycobacterium tuberculosis patients and may involve granuloma formation. (preprints.org)
- Results: The SMRP assay demonstrates intra-assay CVs of 2.3% and 3% (at 4.6 nM and 13.7 nM, respectively), and inter-assay CVs of 3.5% and 3.7% at the same concentrations. (hu-berlin.de)
- To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Osteopontin were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay. (rndsystems.com)
- Circulating concentrations of osteopontin, monocyte chemoattractant protein-1, basic fibroblast growth factor, interleukin-8, and interleukin-10 were significantly reduced only by eplerenone. (ahajournals.org)
- Osteopontin is a 34kDa extracellular matrix protein with a cell-binding domain. (fishersci.com)
- Cell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. (selectscience.net)
- Osteopontin (OPN), an extracellular matrix (ECM) cytokine expressed in HSCs, could drive fibrogenesis by modulating the HSC pro-fibrogenic phenotype and Col 1 expression. (ovid.com)
- Tubulointerstitial injury was assessed by measurement of two independent markers of tubular injury ( i.e ., vimentin and osteopontin), interstitial accumulation of the extracellular matrix components collagen type I, collagen type IV, and laminin, interstitial macrophage infiltration, and renal function. (asnjournals.org)
- Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. (rndsystems.com)
- Osteopontin protein, also known as bone sialoprotein 1 (BSP-1 or BSNP), is a secreted glycoprotein originally isolated from osteoblasts. (clontech.com)
- Conclusions: These assays provide reliable and reproducible quantitation of SMRP and OPN proteins. (hu-berlin.de)
- Osteopontin protein contains an Arg-Gly-Asp (RGD) motif, which is also present in fibronectin, vitronectin, and other extracellular proteins that bind the integrin family of cell surface receptors such as avb3, a5b1, a8b1, and avb5. (clontech.com)
- 11 Integrin receptor α v β 3 can also interact with other blood vessel-associated proteins containing the arginine-glycine-aspartate (RGD) motif such as vitronectin, von Willebrand factor, 13 thrombospondin, 14 fibronectin, 15 laminin, 16 and osteopontin. (ahajournals.org)
- The assays are built on magnetic beads to enable robust quantification of multiple proteins in rat urine samples, providing valuable information throughout drug development - from lead optimization to preclinical and clinical protocol decision making. (bio-rad.com)
- Osteopontin (OPN) serves as a ligand to a number of integrin receptors which are associated with cell motility, chemotaxis, invasion and cell survival. (sigmaaldrich.com)
- At 5 days osteopontin ligand is at a distance from the peri-infarct astrocytes expressing integrin receptor α v β 3 . (ahajournals.org)
- By 15 days astrocytes expressing integrin receptor α v β 3 are localized in an osteopontin-rich region concomitant with formation of the new glial-limiting membrane. (ahajournals.org)
- The de novo expression and interaction of osteopontin ligand with its receptor integrin α v β 3 suggest a role in wound healing after focal stroke. (ahajournals.org)
- Osteopontin and β 3 integrin ligation rapidly increased NF-κB activity as measured by gel shift and reporter activity. (rupress.org)
- Osteopontin (OPN) is an extracellular glycosylated phosphoprotein that promotes cell adhesion by interacting with several integrin receptors. (portlandpress.com)
- In order to asses for αvβ3 integrin independent adhesion, osteolastogenesis assays were performed on Poly-D-lysine coated plates. (bmj.com)
- Using a method of general utility for determining the distribution of unknown integrin ligands in situ and biochemical characterization of these ligands, we identified osteopontin (OPN) as a ligand for alpha8 beta1. (scripps.edu)
- Osteopontin (OPN) is a pro-inflammatory cytokine, and increased circulating levels have been associated with inflammatory muscle diseases and muscular dystrophy ( 10 - 13 ) as well as the onset and progression of Crohn's disease, myocarditis, uveitis, idiopathic retroperitoneal fibrosis, and rheumatoid arthritis ( 14 - 16 ). (frontiersin.org)
- Osteopontin (OPN) is a proinflammatory cytokine with diverse functions. (molvis.org)
- Here, we report that osteopontin (OPN), a cytokine involved in homeostatic and pathophysiologic responses, is constitutively expressed by DCs and regulated in the DC/MSC cocultures depending on the activation state of MSCs. (frontiersin.org)
- The search for a BRMS1-regulated site on the OPN promoter, using luciferase reporter assays of the promoter deletions, identified a novel NF-kappaB site (OPN/NF-kappaB). (nih.gov)
- The osteopontin promoter was cloned into a POL3 reporter vector and transfected into MC3T3-El cells. (wisconsin.edu)
- 8 NFATc3 occupancy at the osteopontin promoter was also demonstrated using chromatin immunoprecipitation assays. (ahajournals.org)
- Soluble mesothelin (SM), megakaryocyte potentiating factor (MPF), and osteopontin (OPN) are blood biomarkers of mesothelioma. (nih.gov)
- The Bio-Plex Pro RBM kidney toxicity assays, developed in partnership with Myriad RBM, comprise a highly relevant set of biomarkers for early detection and characterization of kidney toxicity/injury. (bio-rad.com)
- Putative ovarian cancer biomarkers CA-125, osteopontin, and prolactin were simultaneously quantified using WGM imaging. (ku.edu)
- Arbor Assays is pleased to announce the immediate release of the newest assay kit for reproductive biology research. (arborassays.com)
- The newly improved DetectX ® Protein Kinase A Colorimetric Activity Kit, (K027-H1) , from Arbor Assays provides a sensitive and reliable method for screening inhibitors or activators of PKA and for quantitating PKA activity in a wide range of sample types. (arborassays.com)
- Arbor Assays is proud to offer our Research the Cure™ program for the 8th consecutive year. (arborassays.com)
- Osteopontin level in study 1 remained a significant predictor of relapse-free survival when data were adjusted for age, sex, tumor site, and histologic predictors of relapse. (aacrjournals.org)
- Osteopontin (OPN), a secreted phosphoglycoprotein, has been strongly associated with tumor progression and aggressive cancers. (nih.gov)
- Osteopontin (OPN) is a secreted glycoprotein which has long been associated with tumor metastasis. (biomedcentral.com)
- the relationship between the binding of osteopontin to αv integrins and the acquisition of tumor cell invasive capacity is not known. (rupress.org)
- Finally, in seeded cell migration assays we find that either senescent or Tiam1-deficient fibroblasts induce increased epithelial cell migration that is dependent on fibroblast secretion of osteopontin. (biologists.org)
- These findings indicate that one mechanism by which senescent fibroblasts promote neoplastic progression in associated tumors is through degradation of fibroblast Tiam1 protein and the consequent increase in secretion of osteopontin by fibroblasts. (biologists.org)
- 14. The method of claim 13, wherein the pre-treatment serum osteopontin level is at least 60 ng/ml, and the serum osteopontin level of at least one of said first and second samples is between 30 and 58 ng/ml. (patentsencyclopedia.com)
- The present disclosure relates to occurrence of osteopontin in tissues and fluids of subjects afflicted with pleural mesothelioma, and discloses serum osteopontin levels in three populations: asbestos-exposed subjects without malignancy, unexposed subjects without malignancy, and asbestos-exposed subjects with pleural mesothelioma. (patentsencyclopedia.com)
- Our results further characterize these markers, establish assay performance characteristics, and lay the groundwork for further studies to measure these markers in blood. (hu-berlin.de)
- Thiazides did not affect osteoblast proliferation, but directly stimulated the production of the osteoblast differentiation markers runt-related transcription factor 2 (runx2) and osteopontin. (asnjournals.org)
- The authors concluded that IL6, IL8 and Osteopontin were stronger prognostic markers than actual clinical classifications. (esmo.org)
- Osteopontin (OPN) is a multifunctional adhesive glycoprotein implicated in a variety of proinflammatory as well as neuroprotective and repair-promoting effects in the brain pathophysiology. (arvojournals.org)
- Osteopontin (OPN), also known as early T lymphocyte activation 1 (Eta-1), is a secreted multifunctional glycoprotein. (pacbio.com)
- Cell proliferation was assayed by examining uptake of bromodeoxyuridine (BrdU). (arvojournals.org)
- PC3 cells overexpressing the three OPN-SI were treated with 1×10-6μg/mL of docetaxel and then analysed for cell proliferation and viability using crystal violet and 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide) MMT assays, respectively. (aacrjournals.org)
- The proliferation of BM-MSCs was determined by MTT assay. (bmj.com)
- Lactate dehydrogenase (LDH) release, microculture tetrazolium test (MTT assay) and real-time PCR were employed to evaluate the effects of silibinin on cell cytotoxicity, cell proliferation and expression of various multidrug resistance genes in these cell lines, respectively. (cancerindex.org)
- To test the biocompatibility, Live Cell Imaging (LCI) as well as vitality and proliferation assays were performed. (mdpi.com)
- This assay panel provides high detection sensitivities and broad dynamic ranges. (biolegend.com)
- The present invention relates to reagents and methods for the detection of osteopontin fragments and distinguishing them from each other and from the full-length osteopontin protein. (mmcri.org)
- The present invention also relates to assays for the determination of the presence of osteopontin fragments in samples obtained from subjects and, further, the correlation of osteopontin fragment levels with disease detection, progression and prognosis. (mmcri.org)
- Produced as a 33 kDa nascent polypeptide, osteopontin protein can undergo thrombin cleavage and posttranslational modifications including glycosylation and phosphorylation. (clontech.com)
- O -glycan defects in OPN increased its phosphorylation level, as observed by dephosphorylation assays. (biochemj.org)
- BackgroundHuman milk is rich in osteopontin (OPN), which has immunomodulatory functions.MethodsIn a randomized controlled trial, standard formula (SF) and the same formula with 65 mg of OPN/L (F65) or 130 mg of OPN/L (F130), representing ~50 and 100% of the OPN concentration in human milk, were compared. (nih.gov)
- and (d) concluding that said subject is at increased risk of having pleural mesothelioma if the concentration of osteopontin in said subject sample is higher than the concentration in said control sample or samples. (patentsencyclopedia.com)
- 3. The method of claim 1, wherein the concentration of osteopontin in said sample is determined using an ELISA. (patentsencyclopedia.com)
- 7. The method of claim 1, wherein it is concluded that a subject is at increased risk of having mesothelioma if the osteopontin concentration in said subject sample is higher than the amount in said control sample or samples by a predetermined value. (patentsencyclopedia.com)
- and (d) concluding that said treatment is effective in reducing the severity of mesothelioma if the concentration of osteopontin in said pretreatment sample is higher than the concentration in said first and/or second sample. (patentsencyclopedia.com)
- Expression data were obtained from 74% of primary melanomas and provided confirmatory evidence that osteopontin expression is a prognostic biomarker. (aacrjournals.org)
- Bio-Plex Pro™ human cancer biomarker assays provide high precision and a broad dynamic range in a high throughput cost effective multiplex format. (selectscience.net)