Aspergillus oryzae: An imperfect fungus present on most agricultural seeds and often responsible for the spoilage of seeds in bulk storage. It is also used in the production of fermented food or drink, especially in Japan.Aspergillus: A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.Aspergillus nidulans: A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.Aspergillus niger: An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.Aspergillus fumigatus: A species of imperfect fungi from which the antibiotic fumigatin is obtained. Its spores may cause respiratory infection in birds and mammals.Aspergillus flavus: A species of imperfect fungi which grows on peanuts and other plants and produces the carcinogenic substance aflatoxin. It is also used in the production of the antibiotic flavicin.Pyrithiamine: A thiamine antagonist due to its inhibition of thiamine pyrophosphorylation. It is used to produce thiamine deficiency.alpha-Amylases: Enzymes that catalyze the endohydrolysis of 1,4-alpha-glycosidic linkages in STARCH; GLYCOGEN; and related POLYSACCHARIDES and OLIGOSACCHARIDES containing 3 or more 1,4-alpha-linked D-glucose units.Glucan 1,4-alpha-Glucosidase: An enzyme that catalyzes the hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of polysaccharide chains with the release of beta-glucose. It is also able to hydrolyze 1,6-alpha-glucosidic bonds when the next bond in sequence is 1,4.Xanthomonas: A genus in the family XANTHOMONADACEAE whose cells produce a yellow pigment (Gr. xanthos - yellow). It is pathogenic to plants.Fungal Proteins: Proteins found in any species of fungus.Genes, Fungal: The functional hereditary units of FUNGI.Aspergillosis: Infections with fungi of the genus ASPERGILLUS.Magnaporthe: A genus of FUNGI, in the family Magnaporthaceae of uncertain position (incertae sedis). It is best known for its species, M. grisea, which is one of the most popular experimental organisms of all fungal plant pathogens. Its anamorph is PYRICULARIA GRISEA.DNA, Fungal: Deoxyribonucleic acid that makes up the genetic material of fungi.Spores, Fungal: Reproductive bodies produced by fungi.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Gene Expression Regulation, Fungal: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.Chymosin: The predominant milk-clotting enzyme from the true stomach or abomasum of the suckling calf. It is secreted as an inactive precursor called prorennin and converted in the acid environment of the stomach to the active enzyme. EC 3.4.23.4.Hyphae: Microscopic threadlike filaments in FUNGI that are filled with a layer of protoplasm. Collectively, the hyphae make up the MYCELIUM.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Oryza sativa: Annual cereal grass of the family POACEAE and its edible starchy grain, rice, which is the staple food of roughly one-half of the world's population.Transformation, Genetic: Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.Penicillium: A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.Genome, Fungal: The complete gene complement contained in a set of chromosomes in a fungus.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Starch: Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Industrial Microbiology: The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.Fungi: A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.Arylsulfatases: Enzymes that catalyze the hydrolysis of a phenol sulfate to yield a phenol and sulfate. Arylsulfatase A, B, and C have been separated. A deficiency of arylsulfatases is one of the causes of metachromatic leukodystrophy (LEUKODYSTROPHY, METACHROMATIC). EC 3.1.6.1.Mycelium: The body of a fungus which is made up of HYPHAE.Spontaneous Combustion: A circumstance where a substance or organism takes fire and burns without an exogenous source. Spontaneous human combustion differs from preternatural human combustibility in that in the latter, some spark or trivial flame sets the fire and the body tissues, which have a greatly enhanced inflammability, continue to undergo incineration without any external heat source or combustible materials. (Bergman NA. Spontaneous human combustion: its role in literature and science. Pharos 1988;Fall;51(4):18-21)Allergy and Immunology: A medical specialty concerned with the hypersensitivity of the individual to foreign substances and protection from the resultant infection or disorder.Condiments: Aromatic substances added to food before or after cooking to enhance its flavor. These are usually of vegetable origin.CCAAT-Binding Factor: A heterotrimeric DNA-binding protein that binds to CCAAT motifs in the promoters of eukaryotic genes. It is composed of three subunits: A, B and C.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Transcriptome: The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Ammonium Compounds: Inorganic compounds that include a positively charged tetrahedral nitrogen (ammonium ion) as part of their structure. This class of compounds includes a broad variety of simple ammonium salts and derivatives.MalatesJervell-Lange Nielsen Syndrome: A form of long QT syndrome that is associated with congenital deafness. It is characterized by abnormal cardioelectrophysiology involving the VOLTAGE-GATED POTASSIUM CHANNEL. It results from mutation of KCNQ1 gene (Subtype 1 or JLN1) or the KCNE1 gene (Subtype 2 or JLN2).Nitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Starvation: Lengthy and continuous deprivation of food. (Stedman, 25th ed)Acetylation: Formation of an acetyl derivative. (Stedman, 25th ed)Histone Deacetylases: Deacetylases that remove N-acetyl groups from amino side chains of the amino acids of HISTONES. The enzyme family can be divided into at least three structurally-defined subclasses. Class I and class II deacetylases utilize a zinc-dependent mechanism. The sirtuin histone deacetylases belong to class III and are NAD-dependent enzymes.Histones: Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.Histone Deacetylase Inhibitors: Compounds that inhibit HISTONE DEACETYLASES. This class of drugs may influence gene expression by increasing the level of acetylated HISTONES in specific CHROMATIN domains.

Characterization and expression of the cDNA encoding a new kind of phospholipid transfer protein, the phosphatidylglycerol/phosphatidylinositol transfer protein from Aspergillus oryzae: evidence of a putative membrane targeted phospholipid transfer protein in fungi. (1/357)

The full-length cDNA of a phospholipid transfer protein (PLTP) was isolated from Aspergillus oryzae by a RACE-PCR procedure using degenerated primer pool selected from the N-terminal sequence of the purified phosphatidylinositol/phosphatidylglycerol transfer protein (PG/PI-TP). The cDNA encodes a 173 amino acid protein of 18823 Da. The deduced amino acid sequence from position 38 to 67 is 100% identical to the N-terminal sequence (first 30 amino acids) of the purified PG/PI-TP. This amino acid sequence is preceded by a leader peptide of 37 amino acids which is predicted to be composed of a signal peptide of 21 amino acids followed by an extra-sequence of 16 amino acids, or a membrane anchor protein signal (amino acid 5-29). This strongly suggests that the PG/PI-TP is a targeted protein. The deduced mature protein is 138 amino acids long with a predicted molecular mass of 14933 Da. Comparison of the deduced PG/PI-TP sequence with other polypeptide sequences available in databases revealed a homology with a protein deduced from an open reading frame coding for an unknown protein in Saccharomyces cerevisiae (36% identity and 57% similarity). Apart from this homology, the PG/PI-TP is unique and specific to the filamentous fungi on the basis of comparison of PLTP protein sequences. Northern blot analysis of RNA isolated from A. oryzae cultures grown on glucose or glucose supplemented with phospholipids suggests that the PG/PI-TP is transcribed by only one RNA species and allows us to show that expression of the protein is regulated at the messenger RNA level.  (+info)

Pectin methylesterase gene (pmeA) from Aspergillus oryzae KBN616: its sequence analysis and overexpression, and characterization of the gene product. (2/357)

A gene (pmeA) encoding pectin methylesterase was isolated from a shoyu koji mold, Aspergillus oryzae KBN616, and characterized. The structural gene comprised 1,370 bp with six introns. The PMEA protein consisted of 331 amino acids with a putative signal peptide of 17 amino acids. The deduced amino acid sequence was very similar to those of Aspergillus niger PMEA and Aspergillus aculeatus PME1. The pmeA gene was efficiently expressed under control of the A. oryzae TEF1 gene promoter for purification and characterization of the ezymatic properties. PMEA had a molecular mass of 38.5 kDa, a pH optimum of 5.0, and a temperature optimum of 55 degrees C.  (+info)

Insertion analysis of putative functional elements in the promoter region of the Aspergillus oryzae Taka-amylase A gene (amyB) using a heterologous Aspergillus nidulans amdS-lacZ fusion gene system. (3/357)

Expression of the Taka-amylase A gene (amyB) of Aspergillus oryzae is induced by starch or maltose. The A. oryzae amyB gene promoter contains three highly conserved sequences, designated Regions I, II, and III, compared with promoter regions of the A. oryzae glaA encoding glucoamylase and the agdA encoding alpha-glucosidase. To identify the function of these sequences within the amyB promoter, various fragments containing conserved sequences in the amyB promoter were introduced into the upstream region of the heterologous A. nidulans amdS gene (encoding acetamidase) fused to the Escherichia coli lacZ gene as a reporter. Introduction of the sequence between -290 to -233 (the number indicates the distance in base pairs from the translation initiation point (+1)) containing Region III significantly increased the expression of the lacZ reporter gene in the presence of maltose. The sequence between -377 to -290 containing Region I also increased the lacZ activity, but its maltose inducibility was less than that of Region III. The sequence between -233 to -181 containing Region II had no effect on the expression. These results indicated that Region III is most likely involved in the maltose induction of the amyB gene expression.  (+info)

Delta 9-fatty acid desaturase from arachidonic acid-producing fungus. Unique gene sequence and its heterologous expression in a fungus, Aspergillus. (4/357)

Based on the sequence information for delta 9-desaturase genes (from rat, mouse and yeast), which are involved in the desaturation of palmitic acid and stearic acid to palmitoleic acid and oleic acid, respectively, the corresponding cDNA and genomic gene were cloned from the fungal strain, Mortierella alpina 1S-4, which industrially produces arachidonic acid. There was a cytochrome b5-like domain linked to the carboxyl terminus of this Mortierella desaturase, as also seen in the yeast delta 9-desaturase. The Mortierella delta 9-desaturase genomic gene had only one intron, in which a novel phenomenon was observed: there was a GC-end at the 5'-terminus instead of a GT-end that is, in general, found in introns of eukaryotic genes. The full-length cDNA clone was expressed under the control of an amyB promoter in a filamentous fungus, Aspergillus oryzae, resulting in drastic changes in the fatty acid composition in the transformant cells; the contents of palmitoleic acid (16:1) and oleic acid (18:1) increased significantly, with accompanying decreases in palmitic acid (16:0) and stearic acid (18:0). These changes were controlled by the addition of maltose as a carbon source to the medium. Also, the expression of the gene caused a significant change in the lipid composition in the Aspergillus transformant. Genomic Southern blot analysis of the transformant with the Mortierella delta 9-desaturase gene as a probe confirmed the integration of this gene into the genome of A. oryzae.  (+info)

Site-directed mutagenesis of a possible type 1 copper ligand of bilirubin oxidase; a Met467Gln mutant shows stellacyanin-like properties. (5/357)

In our previous paper, we reported a mutant of recombinant Myrothecium verrucaria bilirubin oxidase, in which the Met467 residue was replaced by Gly [Shimizu, A. et al. (1999) Biochemistry 38, 3034-3042]. This mutant displayed a remarkable reduction in enzymatic activity and an evident decrease in the intensity of the absorption band around 600 nm (type 1 charge transfer transition). In this study, we report the preparation of three Met467 mutants (Met467Gln, Met467His, and Met467Arg) and characterize their enzymatic activities, midpoint potentials, and absorption and ESR spectra. Met467His and Met467Arg show no enzymatic activity and a great reduction in the intensity of the absorption band around 600 nm. Furthermore, their ESR spectra show no type 1 copper signal, but only a type 2 copper signal; however, oxidation by ferricyanide caused the type 1 copper signal to appear. On the other hand, Met467Gln as expressed shows both type 1 and type 2 copper signals in its ESR spectrum, the type 1 copper atom parameters being very different from usual blue copper proteins but very similar to those of stellacyanin. The enzymatic activity of the Met467Gln mutant for bilirubin is quite low (0.3%), but the activity for potassium ferrocyanide is similar (130%) to that of the wild type enzyme. These results indicate that Met467 is important for characterizing the features of the type 1 copper of bilirubin oxidase.  (+info)

Molecular cloning and expression of the novel fungal beta-glucosidase genes from Humicola grisea and Trichoderma reesei. (6/357)

A novel fungal beta-glucosidase gene (bgl4) and its homologue (bgl2) were cloned from the cellulolytic fungi Humicola grisea and Trichoderma reesei, respectively. The deduced amino acid sequences of H. grisea BGL4 and T. reesei BGL2 comprise 476 and 466 amino acids, respectively, and share 73.1% identity. These beta-glucosidases show significant homology to plant beta-glucosidases belonging to the beta-glucosidase A (BGA) family. Both genes were expressed in Aspergillus oryzae, and the recombinant beta-glucosidases were purified. Recombinant H. grisea BGL4 is a thermostable enzyme compared with recombinant T. reesei BGL2. In addition to beta-glucosidase activity, recombinant H. grisea BGL4 showed a significant level of beta-galactosidase activity, while recombinant T. reesei BGL2 showed weak beta-galactosidase activity. Cellulose saccharification by Trichoderma cellulases was improved by the addition of recombinant H. grisea BGL4.  (+info)

Molecular and enzymic properties of recombinant 1, 2-alpha-mannosidase from Aspergillus saitoi overexpressed in Aspergillus oryzae cells. (7/357)

For the construction of an overexpression system of the intracellular 1,2-alpha-mannosidase (EC 3.2.1.113) gene (msdS) from Aspergillus saitoi (now designated Aspergillus phoenicis), the N-terminal signal sequence of the gene was replaced with that of the aspergillopepsin I (EC 3.4.23.18) gene (apnS) signal, one of the same strains as described previously. Then the fused 1, 2-alpha-mannosidase gene (f-msdS) was inserted into the NotI site between P-No8142 and T-agdA in the plasmid pNAN 8142 (9.5 kbp) and thus the Aspergillus oryzae expression plasmid pNAN-AM1 (11.2 kbp) was constructed. The fused f-msdS gene has been overexpressed in a transformant A. oryzae niaD AM1 cell. The recombinant enzyme expressed in A. oryzae cells was purified to homogeneity in two steps. The system is capable of making as much as about 320 mg of the enzyme/litre of culture. The recombinant enzyme has activity with methyl-2-O-alpha-d-mannopyranosyl alpha-D-mannopyranoside at pH 5.0, while no activity was determined with methyl-3-O-alpha-D-mannopyranosyl alpha-D-mannopyranoside or methyl-6-O-alpha-D-mannopyranosyl alpha-D-mannopyranoside. The substrate specificity of the enzyme was analysed by using pyridylaminated (PA)-oligomannose-type sugar chains, Man9-6(GlcNAc)2-PA (Man is mannose; GlcNAc is N-acetylglucosamine). The enzyme hydrolysed Man8GlcNAc2-PA (type 'M8A') fastest, and 'M6C' {Manalpha1-3[Manalpha1-2Manalpha1-3(Manalpha1-6) Manalpha1-6]Manbeta1- 4GlcNAcbeta1-4GlcNAc-PA} slowest, among the PA-sugar chains. Molecular-mass values of the enzyme were determined to be 63 kDa by SDS/PAGE and 65 kDa by gel filtration on Superose 12 respectively. The pI value of the enzyme was 4.6. The N-terminal amino acid sequence of the enzyme was GSTQSRADAIKAAFSHAWDGYLQY, and sequence analysis indicated that the signal peptide from apnS gene was removed. The molar absorption coefficient, epsilon, at 280 nm was determined as 91539 M-1.cm-1. Contents of the secondary structure (alpha-helix, beta-structure and the remainder of the enzyme) by far-UV CD determination were about 55, 38 and 7% respectively. The melting temperature, Tm, of the enzyme was 71 degrees C by differential scanning calorimetry. The calorimetric enthalpy, DeltaHcal, of the enzyme was calculated as 13.3 kJ.kg of protein-1. Determination of 1 g-atom of Ca2+/mol of enzyme was performed by atomic-absorption spectrophotometry.  (+info)

Identification of Delta12-fatty acid desaturase from arachidonic acid-producing mortierella fungus by heterologous expression in the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae. (8/357)

Based on the sequence information for the omega3-desaturase genes (from Brassica napus and Caenorhabditis elegans), which are involved in the desaturation of linoleic acid (Delta9, Delta12-18 : 2) to alpha-linolenic acid (Delta9, Delta12, Delta15-18 : 3), a cDNA was cloned from the filamentous fungal strain, Mortierella alpina 1S-4, which is used industrially to produce arachidonic acid. Homology analysis with protein databases revealed that the amino acid sequence showed 43.7% identity as the highest match with the microsomal omega6-desaturase (from Glycine max, soybean), whereas it exhibited 38.9% identity with the microsomal omega3-desaturase (from soybean). The evolutionary implications of these enzymes will be discussed. The cloned cDNA was confirmed to encode a Delta12-desaturase, which was involved in the desaturation of oleic acid (Delta9-18 : 1) to linoleic acid, by its expression in both the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae. Analysis of the fatty acid composition of yeast and fungus transformants demonstrated that linoleic acid (which was not contained in the control strain of S. cerevisiae) was accumulated in the yeast transformant and that the fungal transformant contained a large amount of linoleic acid (71.9%). Genomic Southern blot analysis of the transformants with the Mortierella Delta12-desaturase gene as a probe confirmed integration of this gene into the genome of A. oryzae. The M. alpina 1S-4 Delta12-desaturase is the first example of a cloned nonplant Delta12-desaturase.  (+info)

*Aspergillus oryzae

... , known in English as koji (Japanese: 麹, Hepburn: kōji), is a filamentous fungus (a mold) used in Chinese and ... The genome of A. oryzae is thus one-third larger than that of two related Aspergillus species, the genetics model organism A. ... Resveratrol can be produced from its glucoside piceid through the process of fermentation by A. oryzae. Akira Endo Aspergillus ... Sake World's description of koji Aspergillus oryzae genome from the Database of Genomes Analysed at NITE (DOGAN). ...

*Sake

The microorganism Aspergillus oryzae (a mold) is sprinkled onto the steamed rice and allowed to ferment for 5-7 days (Uno et al ... Bamforth (2005) places the probable origin of true sake (which is made from rice, water, and kōji mold (麹, Aspergillus oryzae) ... "Genomics of Aspergillus oryzae". Biosci Biotechnol. Biochem. 71(3):646-670. Suzuki K, Asano S, Iijima K, Kitamoto K. (2008). " ... The addition of A. oryzae provides the necessary amylases, glucoamylases, and proteases to hydrolyze the nutrients of the rice ...

*Brinase

It is derived from Aspergillus oryzae. Aspergillus oryzae Frisch EP (July 1972). "Clinical experience with brinase". J. Clin. ... Roschlau WH (July 1972). "Thrombolytic properties and side effects of brinase (fibrinolytic enzyme from Aspergillus oryzae) in ...

*Solid-state fermentation

Aspergillus oryzae Micro-organism Raimbault 1980. Pandey 2003. Singhania et al. 2009. Duchiron & Copinet 2011. Biesebeke et al ... "Aspergillus oryzae in solid-state and submerged fermentations Progress report on a multi-disciplinary project". FEMS Yeast Res ...

*Glucose dehydrogenase (acceptor)

Bak TG (1967). "Studies on glucose dehydrogenase of Aspergillus oryzae. II Purification and physical and chemical properties". ... Other names in common use include glucose dehydrogenase (Aspergillus), glucose dehydrogenase (decarboxylating), and D-glucose:( ...

*Mycoremediation

These fungal strains [Aspergillus oryzae FNBR_L35; Fusarium sp. FNBR_B7, FNBR_LK5 and FNBR_B3; Aspergillus nidulans FNBR_LK1; ... The fungus Aspergillus sclerotiorum CBMAI 849 showed the best performance with regard to pyrene (99.7%) and benzo[a]pyrene ( ... Aspergillus niger recorded maximum decolorization of the dye Basic fuchsin (81.85%) followed by Nigrosin (77.47%), Malachite ... A variety of fungi, such as Pleurotus, Aspergillus, Trichoderma has proven to be effective in the removal of lead, cadmium, ...

*List of domesticated fungi and microorganisms

2005). "Genome sequencing and analysis of Aspergillus oryzae". Nature. 438 (7071): 1157. doi:10.1038/nature04300. PMID 16372010 ...

*Aspergillus nuclease S1

... is an endonuclease enzyme derived from Aspergillus oryzae that splits single-stranded DNA (ssDNA) and ... single strand-specific DNase and Aspergillus oryzae S1 nuclease. Aspergillus nuclease S1 is a monomeric protein of a molecular ... Wiegand, Roger; Nigel Godson; Charles Radding (1975). "Specificity of the S1 Nuclease from Aspergillus oryzae". Journal of ... Aspergillus nuclease S1 is known to be inhibited somewhat by 50 μM ATP and nearly completely by 1 mM ATP. 50% inhibition has ...

*Aspergillopepsin I

Davidson, R.; Gertler, A.; Hofmann, T. (1975). "Aspergillus oryzae' acid proteinase. Purification and properties, and formation ... Aspergillus acid proteinase, Aspergillus aspartic proteinase, Aspergillus awamori acid proteinase, Aspergillus carboxyl ... Aspergillus saitoi acid proteinase, pepsin-type aspartic proteinase, Aspergillus niger acid proteinase, sumizyme AP, proctase P ... Does not clot milk This enzyme is found in a variety of Aspergillus species. Trypsinogen Kovaleva, G.G.; Shimanskaya, M.P.; ...

*Deuterolysin

Nakadai, T.; Nasuno, S.; Iguchi, N. (1973). "Purification and properties of neutral proteinase II from Aspergillus oryzae". ... metalloproteinase from Aspergillus oryzae". Biochemistry (Mosc). 53: 1171-1178. Deuterolysin at the US National Library of ... Sekine, H. (1976). ", Neutral proteinases I and II of Aspergillus sojae action on various substrates". Agric. Biol. Chem. 40: ...

*Fungus

Aspergillus oryzae and Aspergillus sojae". Journal of Food Protection. 70 (12): 2916-34. PMID 18095455. O'Donnell K, Cigelnik E ... Shoyu koji mold (Aspergillus oryzae) is an essential ingredient in brewing Shoyu (soy sauce) and sake, and the preparation of ... Abe K, Gomi K, Hasegawa F, Machida M (September 2006). "Impact of Aspergillus oryzae genomics on industrial production of ... Besides regular sexual reproduction with meiosis, certain fungi, such as those in the genera Penicillium and Aspergillus, may ...

*Piceid

Purification and characterization of piceid-b-D-glucosidase from Aspergillus oryzae. Chunzhi Zhang, Dai Li, Hongshan Yu, Bo ... Polygonum cuspidatum). Resveratrol can be produced from piceid fermented by Aspergillus oryzae. This latter species produces a ... "Biotransformation of piceid in Polygonum cuspidatum to resveratrol by Aspergillus oryzae". Applied Microbiology and ...

*List of unrefined sweeteners

Amazake is made from rice fermented with Koji (Aspergillus oryzae). Many fresh fruits, dried fruits and fruit juices are used ...

*Oligoxyloglucan beta-glycosidase

"A novel enzyme producing isoprimeverose from oligoxyloglucans of Aspergillus oryzae". J. Biochem. 97: 801-810. PMID 4019436. ...

*Aspergillus deoxyribonuclease K1

Kato M, Ikeda Y (1968). "On the deoxyribonucleases, K1 and K2, isolated from mycelia of Aspergillus oryzae. I. Isolation and ... Aspergillus deoxyribonuclease K1 (EC 3.1.22.2, Aspergillus DNase K1) is an enzyme. This enzyme catalyses the following chemical ... Aspergillus deoxyribonuclease K1 at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular ...

*List of sequenced fungi genomes

Aspergillus niger Strain:CBS 513.88, industrial use (2007) Aspergillus oryzae Strain:RIB40, industrial use (2005) Aspergillus ... Aspergillus kawachii IFO 4308, food industry (2011) Aspergillus nidulans Strain:FGSC A4, model organism (2005) Aspergillus ... Aspergillus carbonarius ITEM 5010, food pathogen (2009) Aspergillus clavatus Strain:NRRL1 (2008) Aspergillus fumigatus Strain: ... "Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae". Nature. 438 (7071): 1105-15. ...

*Lactase

"Re: GRAS Notification for Acid Lactase from Aspergillus oryzae Expressed in Aspergillus niger". United States Food and Drug ... such as Aspergillus niger and Aspergillus oryzae. Its primary commercial use, in supplements such as Lacteeze and Lactaid, is ...

*Miso

Aspergillus oryzae onto steamed brown rice. This mould culture comes from dried A. oryzae spores called 'tane-koji' or 'starter ... the fungus Aspergillus oryzae) and sometimes rice, barley, or other ingredients. The result is a thick paste used for sauces ... A. oryzae is an aerobic fungus and is the most active fermenting agents in koji as it produces amylolytic, and proteolytic ... In the past, the natural presence of A. oryzae spores was relied upon to create koji, but because of the difficulty of ...

*Phospholipase A1

"High-level secretory production of phospholipase A1 by Saccharomyces cerevisiae and Aspergillus oryzae". Biosci Biotechnol ... In one particular study, it was found that PLA1 can be produced by S. cerevisiae and A. oryzae. In these PLA1 producing ...

*Akira Endo (biochemist)

Aspergillus oryzae Medicinal molds Monascus purpureus The Science and Technology Foundation of Japan. Japan Prize official ... Soon after, lovastatin, the first commercial statin, was found in the Aspergillus mold. Although mevastatin never became an ...

*Rhizopus oligosporus

Aspergillus oryzae Medicinal molds Saccharomyces boulardii Saccharomyces cerevisiae Shurtleff, W. & Aoyagi, A. 2001. The book ... on growth of Aspergillus flavus and A. parasiticus and accumulation of aflatoxin B1 in groundnut". Mycological Research. 93: ... including the potentially harmful Aspergillus flavus and Staphylococcus aureus. R. oligosporus is at present considered to be a ...

*Huangjiu

Large starter (酒麴, 酒曲; pinyin: jiǔ qū): Wines inoculated using rice cultured with Aspergillus oryzae and yeast. Almost all ... and other grains is inoculated with rice that has already been cultivated with the mold Aspergillus oryzae or molds of the ...

*Meju

Bacteria, mainly Aspergillus oryzae and Bacillus subtilis, are responsible for the fermentation of meju. Well fermented meju ... and undergoes fermentation with Aspergillus oryzae and/or Bacillus subtilis. The word meju (메주) is derived from Middle Korean ...

*Amylolytic process

In sake brewing, the mold Aspergillus oryzae provides amylolysis, and in Tapai, Saccharomyces cerevisiae. Brewing methods. ...

*Kojic acid

... is a chelation agent produced by several species of fungi, especially Aspergillus oryzae, which has the Japanese ... Yabuta T (1924). "The constitution of kojic acid, a gamma-pyrone derivative formed by Aspergillus oryzae from carbohydrates". ...

*Zinc deficiency

Effect of zinc fertilization on rice plants and on the population of the rice-root nematodeHirschmanniella oryzae Jzincournal ... Aspergillus Niger). In 1929 Lutz measured zinc in numerous human tissues using the dithizone technique and estimated total body ...
This page provides information on the status of the A. oryzae RIB40 genome. Data on this page are updated once a day. All the data displayed on this page are available in one or more files (Chromosomal Feature File; GO Annotations File) on the AspGD Download Data page. The Advanced Search tool can also be used to retrieve chromosomal features that match specific criteria. ...
PubMed journal article Growth, ruminal measurements, and health characteristics of Holstein bull calves fed an Aspergillus oryzae fermentation extrac were found in PRIME PubMed. Download Prime PubMed App to iPhone or iPad.
Allcosmeticsource.com Lactase 10,000u/g,5kg/bag,free shipping [EP170508012]- Lactase 10,000u/g,5kg/bag,free shipping What is lactase 10,000u/g? Fungal lactas is an acid lactase that converts lactose into glucose and galactose. The enzyme originates from a special strain of the fungus Aspergillus oryzae. Its activity at low pH (3.5 - 5.5) makes this lactase particularly effective to digest lactose in the stomach and
Aspergillus oryzae is ubiquitous filamentous fungi in nature and has been used in a number of industries such as Japanese traditional fermented foods and pharmaceutical products. In nature and industries, A. oryzae adapt to various environmental conditions by global change of transcriptional regulation. In the previous study, we revealed that the expression of histone acetylation related genes were affected by the growth phase and alteration of growing environment, such as culture conditions and stress exposing. In general, histone acetylation plays the fundamental roles for the genes expression, and closely relates to growth, morphology, differentiation and stress responses. Recently, several reports indicate that histone acetylation also plays important roles in filamentous fungi. In this context, we focused on histone acetylation related genes, particularly histone deacetylases (HDACs). We attempted to disrupt 11 HDACs homologue of A. oryzae and 10 of them were disrupted. However, in the ...
Aspergillus oryzae ATCC ® 14895™ Designation: NRRL 1989 [CBS 134.52, NBIR 2016] Application: produces miso produces soy sauce produces taka-diastase koji produces koji for fermented food products
Oryzin (EC 3.4.21.63, Aspergillus alkaline proteinase, aspergillopeptidase B, API 21, aspergillopepsin B, aspergillopepsin F, Aspergillus candidus alkaline proteinase, Aspergillus flavus alkaline proteinase, Aspergillus melleus semi-alkaline proteinase, Aspergillus oryzae alkaline proteinase, Aspergillus parasiticus alkaline proteinase, Aspergillus serine proteinase, Aspergillus sydowi alkaline proteinase, Aspergillus soya alkaline proteinase, Aspergillus melleus alkaline proteinase, Aspergillus sulphureus alkaline proteinase, prozyme, P 5380, kyorinase, seaprose S, semi-alkaline protease, sumizyme MP, prozyme 10, onoprose, onoprose SA, protease P, promelase) is an enzyme. This enzyme catalyses the following chemical reaction Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyse peptide amides This enzyme is a predominant extracellular alkaline endopeptidase of the mold Aspergillus oryzae. Nakagawa, Y. (1970). "Alkaline proteinases from Aspergillus". ...
Some of the primary ingredients: Fresh chicken meat, chicken meal, salmon meal, turkey meal, pea starch concentrate, green split peas, chicken fat (preserved with mixed tocopherols, rosemary, citric acid), whole eggs, flaxseed, salmon oil, dried alfalfa, carrots, apples, broccoli, bok choy, cabbage, cranberries, Saskatoon berries, dried kelp, dried pumpkin meal, colostrum, sea buckthorn, fenugreek protein meal, dried pomegranate, dried astragalus root, dried milk thistle, glucosamine HCl, chondroitin sulphate, garlic, enzymes (dried aspergillus oryzae fermentation extract, dried aspergillus niger extract, pineapple, dried trichoderma longibrachiatum fermentation product, rhizopus oryzae), probiotics ...
Notes and Questions *How well does it survive or colonize the digestive track? *Would eating these products regularly help those with Celiacs Disease, gluten allergy or casein allergy because of the production on DPPIV? *If it can colonize in the intestines can it also help keep candida albicans in check through its production of cellulase?…
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Chicken meal, turkey meal, salmon meal, de-boned chicken, de-boned turkey, de-boned trout, potatoes, peas, tapioca, lentil beans, chickpeas, pea fibre, chicken fat (preserved with mixed tocopherols), natural chicken flavour, whole dried egg, apples, duck meal, herring meal, flaxseed, salmon oil, alfalfa, de-boned duck, de-boned salmon, sweet potatoes, canola oil (preserved with mixed tocopherols), coconut oil (preserved with mixed tocopherols), potassium chloride, pumpkin, carrots, bananas, blueberries, cranberries, broccoli, spinach, alfalfa sprouts, blackberries, squash, papayas, pomegranate, glucosamine hydrochloride, dried chicory root, dried Lactobacillus acidophilus fermentation product, dried Enterococcus faecium fermentation product, dried Aspergillus niger fermentation product, dried Aspergillus oryzae fermentation product, L-carnitine, vitamins (vitamin A supplement, vitamin D3 supplement, vitamin E supplement, inositol, niacin, L-ascorbyl-2-polyphosphate (a source of vitamin C), ...
Dried Aspergillus Niger fermentation extract, Dried Aspergillus oryzae fermentation extract, Yeast fermentation solubles, Yeast Culture, Organic Chromium, Irradiated Dried Yeast derived from Brewers Yeast (source of mannanoligosac-charides), Yucca Shidigera Extract, Dried Bacilus Subtillis Fermentation Extract, Natural and Artificial Flavoring and Calcium Sulfate ...
A. oryzae, along with most other members of the Aspergillus family, has a hyphae that is hyaline and septate, and conidiophore, which ends at a round-shaped vesicle. From the vesicle extend long filaments called a conodial chain, which appear as long fluffy strands on the surface of the substrate. The spore-bearing cells, or asci, are produced within the ascocarp, or the fruiting body.[3] The primary enzyme secreted by the filamentous fungi is called amylase, which lends a sweet taste to the food it is fermented into. This enzyme is most efficient at a temperature of 35-40 degrees Celsius. Most other enzymes found in A. oryzae grow at a temperature of around 30-35 degrees Celsius.[6] Members of the Aspergillus genus are distinct from other microbes due to the fact that they utilize both a primary and secondary metabolic system. The functionality of the Aspergillus metabolism depends on its carboxylic acids, which break down into fatty acid chains that are composed of a unique set of fatty acid ...
Public RNA-Seq data from: Wang B, Guo G, Wang C, Lin Y, Wang X, Zhao M, Guo Y, He M, Zhang Y, Pan L. Survey of the transcriptome of Aspergillus oryzae via massively parallel mRNA sequencing. Nucleic Acids Res. 2010 Aug;38(15):5075-87. Epub 2010 Apr 14. aoryzae_wang_2010_all_conditions.s,1,2,.fastq - A.oryzae RIB40 RNA-Seq reads reported in Wang et al. 2010. The suffix s1 and s2 indicates the respective end of the paired-end sequencing ...
Ingredients : Corn Syrup, Red Pepper Mixed Seasoning(Red Pepper Powder, Water, Salt, Garlic, Onion), Brown Rice, Water, Fermented Soy Bean Paste(Water, Soy Bean, Soy Bean Cultured with Aspergillus Oryzae, Roasted Soy Bean Powder, Salt, Seed Malt), Isomalto Oligosaccharide, Sea Salt, Red Pepper Powder, Spirits, Yeast Extract Powder(Yeast Extract, Dextrin), Seed Malt(Wheat, Aspergillus Oryzae) Contains: GLUTEN, SOY BEAN, WHEAT ...
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Evans CM, Raclawska DS, Ttofali F, Liptzin DR, Fletcher AA, Harper DN, McGing MA, McElwee MM, Williams OW, Sanchez E, Roy MG, Kindrachuk KN, Wynn TA, Eltzschig HK, Blackburn MR, Tuvim MJ, Janssen WJ, Schwartz DA, Dickey BF. The polymeric mucin Muc5ac is required for allergic airway hyperreactivity. Nat Commun. 2015 Feb 17; 6:6281 ...
In this preferred method in a first step cells are grown in a batch phase until carbon substrate has been consumed, and in a second step the cells are grown to a sufficient cell density to enable heterologous protein production in high amounts. In the first stages of this second phase, heterologous protein production is induced by addition of an inducer to the feed medium. The time it takes to come from zero induction, and thus very low level of heterologous protein production, until induction is maximal, is called the induction phase. This induction phase constitutes the first stage of the feed phase ...
Zloženie:. Sójové boby (46,31%), voda, morská soľ (13,13%), alkohol, fermentačné činidlo: Aspergillus oryzae Tradičný japonský výrobok, ktorý sa podobne ako shoyu používa k dochucovaniu pokrmov.. Tamari na rozdiel od Shoyu má výraznejšiu chuť a hustejšiu konzistenciu tmavšiu farbu a neobsahuje lepok. ...
What 3 foods would you bring on a deserted island? It was in fact a tough question. We are always asked if we could bring only one book, 10 albums, and so on, but never which foods. This would be hard since I love variety, and I wouldnt have anything to do on a island. Yes, there would be a lot of things to do to just simply survive, but surely that couldnt take all day? So I thought about what could give me the biggest bang for my buck.. I thought that there would probably be some produce on the island like leafy greens, roots, and fruit. So I thought, about what I could bring for starches and proteins, and thought rice and beans. Complete protein, okay doing good. But what about the third item, then it hit me- KOJI! So what the heck is koji? It is a strain of bacteria (its scientific name is Aspergillus oryzae) that is purposly grown on rice and soy beans to help preserve them. It is a much more complicated version of our pickles.. The earliest documentation of koji goes back to 300 BCE in ...
Detailed nutritional values of Soy sauce made from soy plus wheat (shoyu). Online analysis information with non-nutrients and nutrients composition facts table for item No. 16123 found in category Legumes and Legume Products. Data contents chart for nutritionists. Food details for SOY SAU MADE FROM SOY&WHEAT (SHOYU) in different kinds of quantities, amounts or measurements.
By Dr. Kamash Ramanathan, EMLab P&K Northwest Regional Laboratory Director. Most of us have read about the problems associated with fungi and their implications for human health. To give due respect to these amazing life forms lets take a moment to look at their beneficial contribution to human society.. Fungi have been a source of food for numerous generations. Today a huge range of edible fungi (mushrooms) are cultivated in a multimillion dollar industry and savored in many parts of the world. Many food products that we use regularly in our daily lives are processed using fungi. Some common examples include soy sauce processed using Aspergillus oryzae and Saccharomyces rouxii, blue cheese using Penicillium roquefortii, etc. Fungi have also been used in activities such as the control of pests, alcohol and beer production, and making bread. But their importance to humanity is not really appreciated until one studies their usage in drug manufacturing. At the beginning of this century, 10 of the ...
Aspergillus amyR protein: a transcriptional activator gene, involved in the amylolytic gene expression in Aspergillus oryzae; GenBank AB012945
Soy and rice are the most frequent substrates for Asian fermented foods.Wheat flour is also often included as an ingredient in many of these prod-ucts.With few exceptions, however, there is a common starting material-koji-that is essential for most Asian fermented foods. As will be described in more detail below, koji is simply a moldy mass of grain, and is derived from the Chinese word meaning moldy grain. In some cases, the koji mold is added to a portion of the raw material which is later added to the remaining substrate (analogous, in a way, to a bulk culture or bread sponge). In other applications, the koji mold is added to the entire raw material. Koji is used not only for production of the many soy sauce-type products that will be described later, but also for sake and related rice wines. Because of the important role koji plays in so many of the products that will described, its manufacture and its microbiological and enzymatic properties are treated separately.. ...
A-amylase is a starch-cleaving enzyme which is used as a flour additive in the baking industry. It is product of Aspergillus oryzae. Flour for bread making must also contain an optimum level of a-amylases. a-amylases are enzymes responsible for the conversion of starch to maltose which is further broken down to form glucose - the sugar required for effective fermentaion of the yeast. If the concentration of a-amylases is too low, too little maltose will be formed and the bread will not rise sufficiently. Too much a-amylase and bread can end up as ‘sticky’ as a result of substances called dextrins forming [1].. Inhalation of dust from different enzymes can be the cause of occupational asthma in exposed workers. Enzymes from different sources are being increasingly used in food. Few cases of food allergy to alpha-amylase induced by eating bread have been reported. Those cases were reported in bakery-related patients and in a pharmaceutical-industry worker [2].. Several studies have ...
Pancreatic VegEnzymes Vegetarian pancreatic enzyme alternative This enzyme blend provides a broad acting formulation derived from the plant sources Aspergillus oryzae and Aspergillus niger. It is a vegetarian alternative to Pancreatic Enzyme Formula for carbohydrate, lipid and protein digestion. These enzymes have several unique properties that enhance their ability to promote the optimal breakdown of foods and nutrients, including broad substrate specificity. This means that these enzymes have the ability to recognize and help digest a large number of target molecules. Plant enzymes are also active in a wide pH range, from about 3.5-8.5, indicating that their actions begin in the stomach and continue in the small intestine, promoting complete digestion. Pure Encapsulations Pancreatic VegEnzymes is comparable in amylase, protease and lipase profile to Pancreatic Enzyme Formula. Each 200 mg capsule of Pancreatic VegEnzymes provides the equivalent of one 500 mg capsule of Pure Encapsulations
This enzyme blend provides a broad acting formulation derived from the plant sources Aspergillus oryzae and Aspergillus niger. It is a vegetarian alternative to Pancreatic Enzyme Formula for carbohydrate, lipid and protein digestion. These enzymes have several unique properties that enhance their ability to promote the optimal breakdown of foods and nutrients, including broad substrate specificity. This means that these enzymes have the ability to recognize and help digest a large number of target molecules. Plant enzymes are also active in a wide pH range, from about 3.5-8.5, indicating that their actions begin in the stomach and continue in the small intestine, promoting complete digestion. Pure Encapsulations Pancreatic VegEnzymes is comparable in amylase, protease and lipase profile to Pancreatic Enzyme Formula ...
Rhozyme P-11™ is not an enzyme but a partially purified extract of Aspergillus oryzae comprising mostly glycosidases with some protease activity, originally produced by Rohm and Hass. There are actually several rhozymes from different species of Aspergillus, such as rhozyme HP150™, each trademarked. Rhozyme is used to break down plant cell walls, which are more polyglycan than protein.. ...
Serrazimes is a proteolytic blend composed of enzymes derived from the edible fungi, Aspergillus oryzae and Aspergillus melleus. Scientific studies indicate that Serrazimes may help maintain and support cardiovascular and lymphatic health through its ability to help break down excess fibrin. Serrazimes may also help support the immune system and promote a healthy response to external stresses. Serrazimes is safe and effective when used as directed, providing enzymatic activity equivalent to Serrapeptidate (aka, Serrapeptase ...
Non-Reducing Oligosaccharide (Produced from Sucrose) Synthesizing Enzyme in ,i,Aspergillus oryzae,/i,:Part 4. Relation between Oligosaccharide-Synthesizing Enzyme and so-called Saccharase (1) (1955 ...
Further evidence on the interspecific protoplast fusion between Aspergillus oryzae and Aspergillus sojae and subsequent haploidization, with special Reference to their production of some hydrolyzing enzymes. (1990 ...
Your doctor will insert a special ultrasound probe in your vagina in much the same way as you insert tampons. Food enzymes can be isolated from pineapples, papayas, figs, pancreas and a mushroom called Aspergillus oryzae. Sometimes, patients will notice continuing pain at the puncture site best herbs for fibroids in the upper thigh. Then, after some quick chatting with my surgeon, I was out.
The leashin mutant is defective in Woronin body inheritance.(A) The lah mutant accumulates HEX assemblies in the apical compartment. The distribution of HEX ass
... - BellaOnline Nutrition Database - BellaOnline is committed to helping our visitors become healthy and happy. Our BellaOnline Nutrition Database will help you choose the healthiest foods for your chosen lifestyle.
There are 10 calories in 1 tablespoon (0.5 fl.oz) of Eden Reduced Sodium Shoyu Soy Sauce. Youd need to walk 3 minutes to burn 10 calories. Visit CalorieKing to see calorie count and nutrient data for all portion sizes.
You can find out about Shoyu, Soy sauce (shiro-shoyu). In this website, nutritional content included in 1878 kinds of foods are introduced by using graphs etc.
A novel yeast expression system was developed in the lab of Prof. Manfred Schmitt from the Department of Applied Molecular Biology of the Saarland University which is based on recombinant derivatives of the yeast (Saccharomyces cerevisiae) virus ScV. The system allows the efficient in vivo assembly of hybrid virus-like particles (VLPs) which have the potential for being used in recombinant vaccine development as well as in heterologous protein production ...
Marco Oldiges is the author of this article in the Journal of Visualized Experiments: Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Rice koji is used for brewing sake 酒, amaze 甘酒(fermented rice poriage) and miso 味噌(fermented soy paste, jiang 醤 in Chinese), wheat koji is mainly for making shoyu 醤油 soy source and bean koji is for making natto 納豆(soy nuggets ...
I was so very fortunate to have worked closely with Cornellia Aihara for eight years, managing the Vega Study Center kitchen, training staff, resident students
Koji Wakamatsu: Japanese filmmaker who directed more than 100 independent films informed by his strongly held antiauthoritarian views. His early films were in the pinku eiga genre of erotic movies...
DDR2, ili CD167b (klaster diferencijacije 167b), je ljudski gen [1], koji je član broj 2 familije diskoidin domen receptora. Receptor tirozin kinaze (RTK) igraju ključnu ulogu u komunikaciji ćelija sa njihovim mikro okruženjem. Ti molekuli su vezani za regulaciju ćelijskog rasta, diferencijacije i metabolizma. U više slučajeva biohemijski mehanizam kojim RTK sprovodi signal kroz membranu je pokazan da se sastoji od ligand indukovane oligomerizacije receptora, i naknadne intracelularna fosforilacije. Ova autofosforilacija dovodi do naknadne fosforilacije citosolnih proteina, kao i do asocijacije sa drugim molekulima koji su vezani za pleiotropske efekte transdukcije signala. RTK proteini imaju trojnu strukturu koja se sastoji od ekstracelularnog, transmembranskog, i citoplazmatičnog regiona. Ovaj gen kodira pripadnika nove RTK podklase sa različitim ekstracelularnim regionom koji okružuje domen sličan faktoru VIII. Alternativno spajanje (engl. splicing) ovog gena na 5 UTR dovodi do ...
Niniejszy serwis internetowy jest przeznaczony dla lekarzy weterynarii oraz hodowców zainteresowanych chowem, hodowlą i szeroko pojętą tematyką zdrowia kóz. Mamy nadzieję, że informacje w nim zawarte są przydatne i mogą być wykorzystane w codziennej pracy. Będziemy wdzięczni za wszelkie uwagi na temat naszego serwisu. Serwis ten jest prowadzony w Zakładzie Chorób Zakaźnych i Epidemiologii Katedry Nauk Klinicznych Wydziału Medycyny Weterynaryjnej SGGW w Warszawie. Powstał w trakcie realizacji grantu KBN 3 P06K 020 22 Dynamika szerzenia się zakaźnego zapalenia stawów i mózgu kóz w Polsce. Serwis redagują: dr Jarosław Kaba lek. wet. Mariusz Nowicki
Formil peptidni receptor (FPR) pripada klasi receptora sa sedam hidrofobnih transmembranskih domena. FPR konformacija je stabilizovana sa nekoliko interakcija. One obuhvataju formiranje potencijalnog sonog mosta između Arg84-Arg205, Lys85-Arg205, i Lys85-Asp284 koji pomažu u određivanju tri-dimenzione strukture transmembranskih domena, kao i pozitivno naelektrisanih ostataka (Arg, Lys) koji interaguju sa negativno naelektrisanim fosfatima. Osim toga ostatak Arg163 može da interaguje sa ligand vezujućim džepom druge ekstracelularne petlje. U pogledu vezivanja formil Met-Leu-Phe peptida, postoji nekoliko dodatnih potencijalnih interakcija kao što su vodonične veze između Arg84 i Lys85 prve ekstracelularne petlje i N-formil grupe liganda, kao i peptidne snove formil Met-Leu-Phe koja može da formira slične interakcije. Za formil-Met funkcionalna grupa liganda je pokazano da formira disulfidne mostove sa Cys ostacima, i interaguje sa Arg163. (Važno je napomenuti da neke od interakcija ...
O nastanku reumatoidnog artritisa se danas zna mnogo više nego ranije, iako je to veoma komplekasn proces. To je zapravo autoimunski odgovor koji uzrokuje nakupljanje ćelija u sinovijalnoj opni zgloba i otpuštanje proizvoda- citokina (TNF, interleukini, faktori rasta) koji doprinose pogoršanju zapaljenja, zadebljanju sinovijalne opne zglobova i razaranju hrskavice i kosti4,5. Krajnji rezultat je razaranje zgloba, okoštavanje i iščašenje.. Postoje značajne varijacije između bolesnika u pogledu brzine oštećenja zglobova, bola i pojave invaliditeta.. U proseku, polovina obolelih postaje nesposobna za rad u roku od 10 godina od postavljanja dijagnoze3.. Epidemiologija. Procenjuje se da u Srbiji od RA boluje oko 30 000 ljudi. Od ovog broja oko 3000 ima teški oblik RA.. Lečenje reumatoidnog artritisa. Smatra se da je oštećenje uzrokovano reumatoidnim artritisom nepovratno, što znači da svi bolesnici zahtevaju hitno i efikasno lečenje da bi se zaustavilo ili usporilo napredovanje ...
Ja vec godinama ne mogu zamisliti zivot bez naocala.Nama koji smo navikli na nosenje naocala ,a i koji bas i ne vidimo bez njih jako je vazno imati dobre okvire!Uz to treba znati da nam bas i nije dostatan samo jedan okvir,Trebamo okvire za razne prigode,razlicita godisnja doba i razlicitu garderobu ...
Mnoge zimske bolesti mogu uzrokovati iste simptome, no za te bolesti možda nije potrebna terapija. Ako Vam je u prošlosti bio propisan antibiotik protiv bolesti, od koje ste se zatim dobro oporavili, uzeti antibiotik u slučaju sličnih simptoma čini se kao najjednostavnije rješenje. No, samo liječnik koji Vas je pregledao može ocijeniti je li za zimsku bolest neophodno liječenje antibioticima ...
Prirodni lekovi pomažu održavanju normalnog nivoa holesterola, bez potrebe da uzimate farmaceutske lekove koji mogu imati opasne nuspojave. Neki od njih su: cr…
Modularni pretvornik omogućuje fleksibilan način upotrebe priključnih modula koji omogućuje objedinjena mjerenja putem digitalnih i analognih senzora, uz mogućnost izbora komunikacije sabirnice. Pretvornik M700 nadzire pH/ORP, otopljeni kisik i plinoviti kisik, CO2 i vodljivost s pomoću digitalnih i analognih senzora.
Organi za razmno avanje Za razliku od ma aka,mu jak psa se lako razlikuje od enke penisom koji visi unutar prepucija s donje strane trbu ne upljine u skrotumu
Active Ingredients per 2 Soft Chews:. Yucca schidigera extract - 200 mg. Parsley leaf - 100 mg. Chamomile - 25 mg. Protease (Aspergillus oryzae) - 2044 HUT. Amylase (Aspergillus oryzae) - 546 DU. Cellulase (Aspergillus niger) - 13.6 CU. Maltase (Aspergillus niger) - 10.2 DP. Invertase (Aspergillus niger) - 0.6 INVU. Lipase (Aspergillus oryzae) - 6.8 LU. Probiotic Blend - 3.5 million CFU/serving. (Lactobacillus acidophilus, Bifidobacterium bifidum, Enterococcus thermophilus). Inactive Ingredients: Brewers Dried Yeast, Calcium Propionate, Canola Oil, Dried Cheese, Glycerin, Green Tea Extract, Liver Meal, Mixed Tocopherols, Natural Flavors, Rosemary Extract, Salmon Oil, Sorbic Acid, Soy Lecithin, Starch, Vegetable Oil, Water.. ...
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.
Yeast Culture, Glucosamine Sulfate, Dried Kelp, Dicalcium Phosphate, Methylsulfonyl Methane, Dried Whey, Ascorbic Acid, New Zealand Green Shell Mussel Powder, Yucca schidigera Extract, Calcium Carbonate, Natural Flavor, Calcium Silicate, Dried Fermentation products of: Lactobacillus acidophilus, Enterococcus faecium, Bifidobacterium thermophilum, Bifidobacterium longum, Dried Aspergillus oryzae Fermentation Extract, Dried Aspergillus niger Fermentation Extract, Hyaluronic Acid, Dried Bacillus subtillis Fermentation Product.. ...
Wheat Oats, Steam Rolled Barley, Shredded Beet Pulp, Processed Grain By-Products, Soybean Meal, Cane Molasses, Corn and Soybean Oil, Rice Bran, Calcium Carbonate, Dicalcium Phosphate, Dried Whey, Yeast Culture, Salt, Magnesium Oxide, Dried Lactobacillus Acidophilus Fermenation Product, Dried Streptococcus Faecium Fermentation Product, Dried Aspergillus Oryzae Fermentation Extract, Dried Aspergullus Niger Fermentation Extract, Vitamin A Acetate, D-Acitvated Animal Sterol (source of vitamin D3), Vitamin E Supplement, Vitamin B12 Supplement, Riboflavin Supplement, Menadione Sodium Bisulfite Complex (source of vitamin K activity), Niacin Supplement, Calcium Pantothenate, Choline Chloride, Folic Acid, Thiamine Monoitrate, Pyridoxine Hydrochloride, Biotin, Sodium Selenite, Zinc Amino Acid Chelate, Thiamine Monotrate, Pyridoxine Hydrochloride, Biotin, Sodium Selenite, Zinc Amino Chelate, Zinc Sulfate, Iron Amino Acid Chelate, Ferrous Sulfate, Manganese Amino Acid Chelate, Manganes Sulfate, Copper Amino ...
Sigma-Aldrich offers abstracts and full-text articles by [Christoph Knuf, Intawat Nookaew, Stephen H Brown, Michael McCulloch, Alan Berry, Jens Nielsen].
Matrix contains a highly concentrated combination of isolated natural plant extracts; live and naturally occurring, host-specific strains of beneficial bacteria; calcium silicate; active live yeast; highly refined yucca extract; and aspergillus oryzae.. Multidimensional and unique combination of host specific beneficial bacteria, natural vasodilators, live yeast, yucca, and starch digesting enzymes.. The primary components which Matrix Technology incorporates are natural vasodilators from selected plant extracts host-specific, proprietary, encapsulated, beneficial bacteria; live cell yeast; aspergillus oryzae; yucca extract; diatomaceous earth; and natural toxin mitigants.. Vasodilation diagram. ...
A popular feed additive:. As a matter of fact, probiozyme is one of the feed additives, which contains appropriate Probiotic organism. This kind of organism is found to be very useful in improving the stability of gut microflora. This additive is also found to be extremely beneficial for improving the health of the digestive system of the body. At the same time, bioceuticals probiozyme also assists in assimilation of nutrients in the body. Thereby, probiozyme helps in nourishing the body. It is also found to be beneficial in treating diarrhoea and antibiotic associated diarrhoea (AAD).. Composition of the capsule:. On the other hand, the BioCeuticals ProbioZyme 45 capsules are known to have a healthy composition of probiotic which is dairy free. In addition to this, the capsule also contains plant derived enzymes. Some of the enzymes present in the capsule are Probiozyme Amylase, which is secured from Aspergillus oryzae, Tilactase got from Aspergillus oryzae, Bromelains from Ananas comosus and ...
shio koji is another Japanese fermented condiment that can deepen the flavour of your dishes.. Like miso this seasoning is versatile and rich in live enzymes.. Koji is steamed rice that is cultured with special mold/starter, Aspergillus oryzae. This is essential for making amazake, miso, mirin, sake, tamari and more.. Shio Koji probably is so easy to make and add saltiness , sweetness and umami to your chosen dishes easily.. koji 200g. salt 120g. water 1/2 cup. ...
During the last decade Bacillus megaterium was systemically developed for the gram per liter production of recombinant proteins. In contrast to the usual bacterial gene expression scenario, recombinant protein production systems often rely on multicopy plasmid encoded promoters, repressors and target genes. During production of the green fluorescent model protein (GFP) as a model culture heterogeneity was observed at the single cell level using time-lapse microscopy. The two subpopulations (producing versus low-producing) were separated by cell sorting and initially analyzed for limiting components of the expression system via quantitative RT-PCR. Further cultivation of both subpopulations revealed a systematic reajustment of their subpopulation distributions, indicationg bistability. An initial model was deduced as working hypothesis to identify the molecular basis for the observed phenotype and to improve our mathematical model ...
β-N-Acetylhexosaminidase (GH20) from the filamentous fungus Talaromyces flavus, previously identified as a prominent enzyme in the biosynthesis of modified glycosides, lacks a high resolution three-dimensional structure so far. Despite of high sequence identity to previously reported Aspergillus oryzae and Penicilluim oxalicum β-N-acetylhexosaminidases, this enzyme tolerates significantly better substrate modification. Understanding of key structural features, prediction of effective mutants and potential substrate characteristics prior to their synthesis are of general interest. Computational methods including homology modeling and molecular dynamics simulations were applied to shad light on the structure-activity relationship in the enzyme. Primary sequence analysis revealed some variable regions able to influence difference in substrate affinity of hexosaminidases. Moreover, docking in combination with consequent molecular dynamics simulations of C-6 modified glycosides enabled us to identify the
Fermentation is one of the important processes in the manufacturing of shochu. Shochu is created through the alcoholic fermentation of raw materials such as potatoes, wheat, or rice.. The raw materials used to make shochu cannot be directly transformed into alcohol. An enzyme in aspergillus oryzae mold is used to convert the starches found into the raw materials into sugar, then yeast works to create alcohol. Carbon dioxide is produced as the alcohol is produced.. Fermentation, which relies on the power of microorganisms invisible to the naked eye, can be considered an important part in the production of delicious-tasting sochu.. Shochu brewers pay attention to the sounds inside the fermentation pot. Checking the "breathing" of the fermentation, they look after the progress of the mash. Our "fermentation sounds" are recordings of the sounds of this breathing of the fermentation. A special underwater microphone is inserted inside the pots or tanks to pick up these sounds.. These fermentation ...
Aspergillus fumigatus AFPAB1 is the ortholog of the Aspergillus oryzae cytoplasmic messenger ribonucleoprotein granules AOPAB1 that function to depress the initiation of translation during stress. A. fumigatus can regulate its cellular physiology in response to environmental stresses, but there has been no research on Pab1 in A. fumigatus. The associated gene afpab1 was replaced with a hygromycin-selectable marker to generate the strain Δafpab1. Phenotypic analysis showed that the Δafpab1 grew more weakly than the wild-type strain. Also the germination rate of Δafpab1 was decreased when exposed to oxidative stress. The morphology of Δafpab1 spores also showed great changes. The killing rate of Δafpab1 by RAW264.7 murine macrophage cells was increased, and the reactive oxygen species (ROS) scavenging ability of Δafpab1 was decreased. Pathogenicity testing showed that the deletion strain had decreased virulence. Therefore, we conclude that afpab1 activity is correlated with susceptibility to
Fusidane-type antibiotics represented by helvolic acid, fusidic acid and cephalosporin P1 are a class of bacteriostatic agents, which have drawn renewed attention because they have no cross-resistance to commonly used antibiotics. However, their biosynthesis is poorly understood. Here, we perform a stepwise introduction of the nine genes from the proposed gene cluster for helvolic acid into Aspergillus oryzae NSAR1, which enables us to isolate helvolic acid (~20 mg L-1) and its 21 derivatives. Anti-Staphylococcus aureus assay reveals that the antibacterial activity of three intermediates is even stronger than that of helvolic acid ...
Sumathi, S and Dasgupta, D and Vaidyanathan, CS (1998) Chemical modification of 3-HBA-6-hydroxylase by phenylglyoxal: Kinetic acid physicochemical studies on the modified enzyme. In: Indian Journal of Biochemistry and Biophysics, 35 (5). pp. 266-272. Santha, R and Rao, Appaji N and Vaidyanathan, CS (1996) Identification of the active-site peptide of 2,3-dihydroxybenzoic acid decarboxylase from Aspergillus oryzae. In: Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1293 (2). pp. 191-200. Mahajan, MC and Phale, PS and Vaidyanathan, CS (1994) Evidence for the involvement of multiple pathways in the biodegradation of 1- and 2-methylnaphthalene by pseudomonas putida CSV86. In: Archives of microbiology, 161 (5). pp. 425-433. Bhat, MA and Tsuda, M and Horiike, K and Nozaki, M and Vaidyanathan, CS and Nakazawa, T (1994) Identification and characterization of a new plasmid carrying genes for degradation of 2,4-dichlorophenoxyacetate from Pseudomonas cepacia CSV90. In: ...
What are we trying to do while making koji?. OK, so what exactly are we trying to do when making koji? Well, to examine this we need to consider the role koji plays in Sake Brewing. In sake brewing we use koji to provide a wide variety of products. These include products that provide flavor and aroma elements as well as enzymes which degrade proteins and starches into smaller component parts. For example proteins are disassembled into peptides and amino acids while starches are converted into smaller starches, dextrins and sugars.. Rice starts out with 7% to 8% protein, but the higher polished the rice the less protein will be left. However, even with lower levels of milling we do not focus on kojis production of enzymes to break down this protein. Rice starch is our main focus and needs to be broken down as effectively as possible into sugars. Koji produces alpha, beta and gamma amylase. Depending on how we culture the koji, we can emphasize protein or starch degrading enzymes. High ...
ID G4MMX1_MAGO7 Unreviewed; 824 AA. AC G4MMX1; DT 14-DEC-2011, integrated into UniProtKB/TrEMBL. DT 14-DEC-2011, sequence version 1. DT 20-DEC-2017, entry version 38. DE SubName: Full=ATP-dependent permease MDL2 {ECO:0000313,EMBL:EHA56993.1}; GN ORFNames=MGG_06878 {ECO:0000313,EMBL:EHA56993.1}; OS Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice OS blast fungus) (Pyricularia oryzae). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; OC Sordariomycetes; Sordariomycetidae; Magnaporthales; Magnaporthaceae; OC Magnaporthe. OX NCBI_TaxID=242507 {ECO:0000313,EMBL:EHA56993.1, ECO:0000313,Proteomes:UP000009058}; RN [1] {ECO:0000313,EMBL:EHA56993.1, ECO:0000313,Proteomes:UP000009058} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958 RC {ECO:0000313,Proteomes:UP000009058}; RX PubMed=15846337; DOI=10.1038/nature03449; RA Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., RA Orbach M.J., Thon M., Kulkarni R., Xu J.R., Pan ...
The increasing availability of omics databases provide important platforms for yeast engineering strategies since they offer a lot of information on the physiology of the cells under diverse growth conditions, including environmental stresses. Notably, only a few of these approaches have considered a performance under recombinant protein production conditions. Recently, we have identified a beneficial effect of low oxygen availability on the expression of a human Fab fragment in Pichia pastoris. Transcriptional analysis and data mining allowed for the selection of potential targets for strain improvement. A first selection of these candidates has been evaluated as recombinant protein secretion enhancers. Based on previous transcriptomics analyses, we selected 8 genes for co-expression in the P. pastoris strain already secreting a recombinant Fab fragment. Notably, WSC4 (which is involved in trafficking through the ER) has been identified as a novel potential target gene for strain improvement, with up
1. The somatic chromosome number for the female of Calandra oryzae is twelve; for the male, eleven. The latter is presumably of XO composition.. 2. In both sexes the haploid number of loosely paired threads is present at early diplotene. Upon condensation, the exconjugant chromosomes undergo a temporary complete dissociation into their univalent constituents, so that the diploid number of separate elements is reconstituted.. 3. At the equatorial plate stage the twelve univalent chromosomes associate once again into six bivalents.. 4. In the female this is followed by a precocious separation of the anaphase chromatids, so that by the conclusion of the first meiotic division the diploid number of chromosome elements is evident at both ends of the spindle. There is no comparable process during the course of spermatogenesis; at anaphase I the haploid set of chromosomes passes to each pole, the nuclei so produced entering a resting condition.. ...
Tražite li partnera na putu k formi, upoznali bismo vas s Vitom Elite Step Through. Konstruirana je od našeg E5 Premium aluminija, stoga je garantirano biti otporna i čvrsta. Ova Vita ima step through dizajn rame koja olakšava sjedanje na bicikl i silazak s njega, kao i spuštanje noge. Znajući da ćete se mučiti iz kilometra u kilometar, odlučili smo vam pružiti pouzdanu skupinu dijelova dizajniranih posebno za žene koji će biti uz vas na duge pruge. Povrh toga, ubacili smo mnogo udobnih detalja koji ne utječu na performanse bicikla, poput naše ženske Fitness geometrije sa Zertz umecima koji smanjuju vibracije. Dodajte tome hidraulične disk kočnice koje na mjestu stanu i imate bicikl koji omogućava savršen osjećaj udobnosti i kontrole dok se znojite.
While there is not a lot to report on the free agent front, we can relay a report from FoxSports.coms Ken Rosenthal, who mentioned veteran righthander Koji Uehara as a reliever who interests the Phillies. - David Murphy, Philadelphia Daily News
Uwagi: Epub ahead of print 19 JUna 2011; grant Medical University of Lodz, Poland (No. 502-11-592; 502-11-744; 503-1034-3), grants from the Polish National Committee of Scientific Research (KBN, Warsaw, Poland; No. 2 P05E 099 28 and No. 2 P05A 015 29). ...
04/2013:40100]. Gdje slovo R (cijelo ili u kurzivu) slijedi ime tvari ili otopine, to zna i da je reagens uklju en u sljede i popis. Specifikacije dane za reagense ne garantiraju nu no njihovu kakvo u za uporabu u lijekovima.. Unutar opisa svakog reagensa postoji sedmeroznamenkasta ifra napisana kurzivom (npr. 1002501). Ovaj broj, koji ostaje nepromijenjen za dani reagens tijekom narednih revizija popisa se koristi u identifikacijske svrhe Tajni tva te korisnicima Farmakopeje tako er mo e biti koristan, npr. u upravljanju zalihama reagensa. Opis mo e uklju iti CAS (Chemical Abstract Service Registry Number) broj, koji se mo e prepoznati po tipi nom formatu, npr. 9002-93-1. Neki od reagensa uklju enih u popis su toksi ni te se njima mora rukovati u skladu s laboratorijskom praksom dobre kontrole kakvo e.. Reagensi u vodenoj otopini se pripremaju uporabom vode R. Gdje je otopina reagensa opisana uporabom izraza poput kloridna kiselina (10 g/L HCl) , otopina se pripremi odgovaraju im razrje ivanjem ...
U ovom završnom radu biti će objašnjena autentikacija i autorizacija, te nužne metode za uspostavljanje sigurnosti i zaštite podataka korisnika. Kriptografija i šifriranje osnovni su oblici zaštite podataka. Provedba zaštite podataka provodi se kriptografskim algoritmima. U radu su opisane jednosmjerne funkcije za izračun sažetka poruke, simetrični, te asimetrični algoritmi. Sve metode zaštite podataka temelje se na kriptografskim algoritmima pomoću kojih poruke postaju besmislene onima koji nisu autorizirani korisnici. U radu su opisani najčešći napadi na autentikaciju i autorizaciju korisnika. Opisane su mjere zaštite te sigurnosni protokoli koji štite podatke od neovlaštenog pristupa. U praktičnom dijelu ovog završnog rada objašnjen je i prikazan proces autentikacije korisnika primjenom HMAC simetričnog algoritma ...
Mehanizmi koji kontroliraju normalnu diferencijaciju i aktivaciju B-stanice često su u stanici limfoma kako bi u konačnici transformirana stanica imala neograničen rast i razvoj. B-stanice osobito su podložne malignim transformacijama zato što procesi koji dovode do velike raznolikosti imunoglobulina, koja je potrebna u normalnom imunološkom odgovoru, često vode do kromosomskih translokacija i onkogenih mutacija koje uključuju jake promotore Ig gena i mnoge tumor supresorske gene, kao i onkogene. Takvi događaji često vode do blokiranja diferencijacije, sprječavanja apoptoze i/ili poticanja proliferacije B-stanice koja konačno može biti prvi događaj u tranformaciji B-stanice u tumorsku stanicu. Dodatno, mnogi B-stanični limfomi trebaju ekspresiju BCR-a kako bi preživjeli, u nekim limfomima aktivacija stanice preko signalnog puta B-staničnog receptora te u nekima specifični faktori mikrookoliša tumora imaju važnu ulogu u tumorogenezi. Istraživanja na polju strukturne i ...
Pjesme koje već duže nisu izvodili na koncertima, pokoje iznenađenje i vrhunska zabava. To je kultni britanski bend Placebo obećao uoči svog zagrebačkog koncerta, jedinog u regiji. Svi koji su se sinoć zatekli u KC Dražen Petrović, dobili su upravo to, jer proslava rođendana bila je idealna prilika za presjek jedne bogate karijere. A Placebo […]. ...
Po tovani posetioci, ako elite da posetite neki od prilo enih linkova, u inite to klikom na logo sli icu (mini logo banner). Samo tada, va klik e biti registrovan i done e odgovaraju e poene izabranom sajtu. Zahvaljuju i va im klikovima, va i omiljeni sajtovi se mogu na i na TOP 5 listi (koja se dinami ki odre uje). TOP 5 listu prikazujemo na svim stranicama na eg sajta. Pored toga, sajtovi koji se nalaze na TOP 5 listi, prikazuju se i na svim sajtovima na kojima se nalazi postavljena na a markica (R)MONITOR-a. eleli smo da webmasterima sajtova obezbedimo i standardne linkove (bez redirekcije). Klik na neki od tih linkova (nalaze se u drugoj koloni) - sistem ne registruje i samim tim, takav klik ne uti e na poziciju sajta u okviru TOP 5 liste. ...
Koji Fukadas 2010 black comedy, Kantai (Hospitalité), about a smiling stranger who wanders into the lives of a middle-class family and wreaks havoc, has
Sav sadrzaj koji se nalazi na nasem forumu sluzi iskljucivo u edukativne svrhe, svaka upotreba i koristenje nelegalnih programa i televizijskih streamova je zakonom kaznjiva, kupite ili se preplatite na legalan program ili televizijski paket ...
Sav sadrzaj koji se nalazi na nasem forumu sluzi iskljucivo u edukativne svrhe, svaka upotreba i koristenje nelegalnih programa i televizijskih streamova je zakonom kaznjiva, kupite ili se preplatite na legalan program ili televizijski paket ...
Chicken Meal, Turkey Meal, Brown Rice, White Rice, Lamb Meal, Chicken Fat (preserved with Mixed Tocopherols), Herring Meal, Flax Seed, Sun Cured Alfalfa Meal, Sunflower Oil, Chicken, Lecithin, Monocalcium Phosphate, Potassium Chloride, Choline Chloride, Linoleic Acid, Rosemary Extract, Sage Extract, Dried Enterococcus Faecium, Dried Lactobacillus Acidophilus Fermentation Product, Dried Aspergillus Oryzae Fermentation Extract, Dried Bacillus Subtilis Fermentation Extract, Inulin (from Chicory root), Saccharomyces Cerevisiae Fermentation Solubles, Yucca Schidigera Extract, Mixed Tocopherols (source of Vitamin E), Zinc Amino Acid Chelate, Manganese Amino Acid Chelate, Iron Amino Acid Chelate, Copper Amino Acid Chelate, Cobalt Amino Acid Chelate, Vitamin A Supplement, Vitamin D3 Supplement, Ascorbic Acid (source of Vitamin C), Niacin, Thiamine Mononitrate (Vitamin B1), Riboflavin (source of B2), Beta Carotene, Calcium Pantothenate, Pyridoxine Hydrochloride (Vitamin B6), Calcium Iodate, Folic Acid, ...
0072] First, the water extract of Lentinus edodes, the water extract of Pleurotus ostreatus, and the water extract of Flammulina velutipes were added into 135 shake flasks so that each set of 45 shake flasks contain the water extract of the same mushroom product. The spore inoculums of Aspergillus oryzae ATCC 26831 (BCRC 30235), which were obtained from the preceding section entitled "A. Preparation of spore inoculums of Aspergillus oryzae BCRC 30235", were inoculated into the three sets of the 45 shake flasks so that the resultant mixtures in each set of the 45 shake flasks had three final spore inoculum concentrations (3.4×105 spore/mL, 1.3×106 spore/mL, and 3.4×106 spore/mL). Namely, among the mixtures in each set of the 45 shake flasks, the mixtures in each subset of 15 shake flasks were formed to have the same spore inoculum concentration. The shake flasks were placed in a thermostatic incubator shaker with conditions set at 25° C. and 150 rpm so as to allow fermentation culture. At ...
Isopullulanase (IPU) from Aspergillus niger A.T.C.C. (American Type Culture Collection) 9642 hydrolyses pullulan to isopanose. IPU is important for the production of isopanose and is used in the structural analysis of oligosaccharides with α-1,4 and α-1,6 glucosidic linkages. We have isolated the ipuA gene encoding IPU from the filamentous fungi A. niger A.T.C.C. 9642. The ipuA gene encodes an open reading frame of 1695 bp (564 amino acids). IPU contained a signal sequence of 19 amino acids, and the molecular mass of the mature form was calculated to be 59 kDa. IPU has no amino-acid-sequence similarity with the other pullulan-hydrolysing enzymes, which are pullulanase, neopullulanase and glucoamylase. However, IPU showed a high amino-acid-sequence similarity with dextranases from Penicillium minioluteum (61%) and Arthrobacter sp. (56%). When the ipuA gene was expressed in Aspergillus oryzae, the expressed protein (recombinant IPU) had IPU activity and was immunologically reactive with ...
There is 1 calorie in a 1/2 teaspoon of Soy Sauce (Shoyu) (Low Sodium). Get full nutrition facts and other common serving sizes of Soy Sauce (Shoyu) (Low Sodium) including 1 tsp and 1 tbsp.
Preferred for the invention is CBMs of Carbohydrate-Binding Module Family 20. CBMs of Carbohydrate-Binding Module Family 20 suitable for the invention may be derived from glucoamylases of Aspergillus awamori (SWISSPROT Q12537), Aspergillus kawachii (SWISSPROT P23176), Aspergillus niger (SWISSPROT P04064), Aspergillus oryzae (SWISSPROT P36914), from alpha-amylases of Aspergillus kawachii (EMBL#AB008370), Aspergillus nidulans (NCBI AAF17100.1), from beta-amylases of Bacillus cereus (SWISSPROT P36924), or from CGTases of Bacillus circulans (SWISSPROT P43379). Preferred is a CBM from the alpha-amyiase of Aspergillus kawachii (EMBL:#AB008370) as well as CBMs having at least 50%, 60%, 70%, 80% or even at least 90% homology to the CBM of the alpha-amyiase of Aspergillus kawachii (EMBL#AB008370), i.e. a CBM having at least 50%, 60%, 70%, 80% or even at least 90% homology to the amino acid sequence of SEQ ID NO:6. Also preferred for the invention are the CBMs of Carbohydrate-Binding Module Family 20 ...
Batch cultures of mixed rumen micro-organisms were used to evaluate varying enzyme products with high xylanase activity (EPX), four of which were recombinant single xylanase activity developmental enzyme products (EPX1-EPX4, products of xylanase genes derived from Trichoderma harzianum, Trichoderma reesei, Orpinomyces and Aspergillus oryzae, respectively), for their potential to improve in vitro ruminal fermentation of three forages [maize (Zea mays) stover (MS), rice (Oryza sativa) straw (RS) and Guimu No. 1 grass (Pennisetum americanum×Pennisetum purpureum, GM)]. The enzyme product EPX5, derived from Trichoderma longibrachiatum, was used as a positive control that could improve in vitro fermentation of forages. Enzymes were supplied at dose rates of 0 (control), 20 (low), 50 (medium) and 80 (high) enzymic units of xylanase/g of dry matter (DM). There were no interactions between EPX and dose for the fermentation characteristics evaluated. Increasing EPX dose linearly increased gas production ...
Catzymes Kidney Support is an all natural proprietary blend of phyto-nutrients, Methionine, Vitamins, Minerals and Amino Acids designed to nutritionally support renal function. Animals with renal system issues need nutrients delivered in a manner different than their healthy counterparts. Contains Pre-digested Whitefish, Choline and live microorganisms as well. This supportive formula contains vitamins, minerals, trace minerals, amino acids, inert fats, and predigested whitefish needed for optimum renal function and health. It delivers these essential elements in a format that is less demanding on the renal system.. Ingredients: Active Dry Yeast, Ascorbic Acid, a-tocopherol, Biotin, Calcium Ascorbate, Choline Chloride, Cobalt Sulfate, Dehydrated Animal Fat, Dehydrated Parmesan Cheese, Dried Aspergillus niger Fermentation Extract, Dried Aspergillus oryzae ermentation Extract, Dried Bacillus subtilis Fermentation Product, Dried Bifidobacterium adolescentis Fermentation Product, Dried ...
Allcosmeticsource.com Fungal amylase 1000000u/g,5kg/bag,free shipping [EP170508011]- Fungal amylase 1000000u/g,5kg/bag,free shipping What is Fungal amylase 1000000u/g? Fungal amylase, also known as fungal alpha amylase (1, 4- alpha -D- glucan hydrolase), is refined by Aspergillus oryzae Wahl deep culture, extraction and other processes. The enzyme is endo amylase, which can rapidly hydrolyze the starch, amylose and amylopectin aqueous solution of alpha -1,
We investigated the effect of proteases, widely used for neuron isolation in electrophysiological studies, on the amplitude and kinetic characteristics of persistent sodium current (INaP) in hippocampal CA1 pyramidal neurons. Properties of INaP were studied on neurons isolated by mechanical treatment (control group) and by mechanical and enzymatic treatment using pronase E (from Streptomyces griseus) or protease type XXIII (from Aspergillus oryzae). We show that in neurons isolated with pronase E kinetic of activation and density of INaP was unaltered. Enzymatic treatment with protease type XXIII did not alter INaP activation but result in significant decrease in INaP density. Our data indicates that enzymatic treatment using pronase E for neuron isolation is preferable for investigation of INaP ...
Translocated chromosomal duplications occur spontaneously in many organisms; segmental duplications of large chromosomal regions are expected to result in phenotypic changes because of gene dosage effects. Therefore, experimentally generated segmental duplications in targeted chromosomal regions can be used to study phenotypic changes and determine the functions of unknown genes in these regions. Previously, we performed tandem duplication of a targeted chromosomal segment in Aspergillus oryzae. However, in tandem chromosomal duplication, duplication of chromosomal ends and multiple chromosomal duplication are difficult. In this study, we aimed to generate fungal strains with a translocated duplication or triplication of a targeted chromosomal region via break-induced replication. Double-strand breaks were introduced into chromosomes of parental strains by treating protoplast cells with I-SceI meganuclease. Subsequently, strains were generated by nonreciprocal translocation of a 1.4-Mb duplicated region
ID A0A081BHI0_9LACO Unreviewed; 447 AA. AC A0A081BHI0; DT 29-OCT-2014, integrated into UniProtKB/TrEMBL. DT 29-OCT-2014, sequence version 1. DT 25-OCT-2017, entry version 26. DE RecName: Full=Chromosomal replication initiator protein DnaA {ECO:0000256,HAMAP-Rule:MF_00377, ECO:0000256,RuleBase:RU000577}; GN Name=dnaA {ECO:0000256,HAMAP-Rule:MF_00377, GN ECO:0000313,EMBL:GAK47498.1}; GN ORFNames=LOSG293_070370 {ECO:0000313,EMBL:GAK47498.1}; OS Lactobacillus oryzae JCM 18671. OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; OC Lactobacillus. OX NCBI_TaxID=1291743 {ECO:0000313,EMBL:GAK47498.1, ECO:0000313,Proteomes:UP000028700}; RN [1] {ECO:0000313,EMBL:GAK47498.1, ECO:0000313,Proteomes:UP000028700} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=SG293 {ECO:0000313,EMBL:GAK47498.1, RC ECO:0000313,Proteomes:UP000028700}; RA Tanizawa Y., Fujisawa T., Mochizuki T., Kaminuma E., Nakamura Y., RA Tohno M.; RT "Draft Genome Sequence of Lactobacillus oryzae Strain SG293T."; ...
article{569d6702-1354-4b3b-8b97-3c11c9807854, abstract = {Metabolic stress is a phenomenon often discussed in conjunction with recombinant protein production in Escherichia coli. This investigation shows how heterologous protein production, and the presence of host cell proteases is related to: i) IPTG induction, ii) cell mass concentration at the time of induction and iii) the presence of metabolites (glutamic acid or those from TSB) during the post induction phase of high-cell-density (HCD) fed-batch cultivations. Two thermostable xylanase variants and one thermostable cellulase, all originating from Rhodothermus marinus were expressed in E. coli strain BL21 (DE3). A three-fold difference in the specific activity of both xylanase variants [between 7000 and 21000 U / (g cdw)], was observed under the different conditions tested. Upon induction at high cell-mass concentrations employing a nutrient feed devoid of the metabolites above, the specific activity of the xylanase variants, was initially ...
BACKGROUND: Fungi are important players in the turnover of plant biomass because they produce a broad range of degradative enzymes. Aspergillus nidulans, a well-studied saprophyte and close homologue to industrially important species such as A. niger and A. oryzae, was selected for this study. RESULTS: A. nidulans was grown on sorghum stover under solid-state culture conditions for 1, 2, 3, 5, 7 a ...
Identification of Rhizoctonia solani, R. oryzae and R. oryzae-sativae, components of the rice sheath disease complex, is extremely difficult and often inaccurate and as a result may hinder the success of extensive breeding programmes throughout Asia. In this study, primers designed from unique regions within the rDNA internal transcribed spacers have been used to develop a rapid PCR-based diagnostic test to provide an accurate identification of the species on rice. Tests on the specificity of the primers concerned showed that they provide the means for accurate identification of the Rhizoctonia species responsible for sheath diseases in rice. ...
Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity.(Jpn. Agric. Res. Q.) [2005] ...
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Receptor insulinu sličnog faktora rasta 1 je protein koji je prisutan na površini ljudskih ćelija. On je transmembranski receptor koji se aktivira IGF-1 hormonom (insulinu sličan faktor rasta 1) i srodnim hormonom IGF-2. On pripada velikoj klasi tirozinsko kinaznih receptora . Ovaj receptor posreduje dejstvo IGF-1, koji je polipeptidni proteinski hormon sličan insulinu. IGF-1 utiče na razviće i nastavlja da vrši anaboličko dejstvo kod odraslih. On može da indukuje hipertroifju skeletalnih mišića i drugih ciljnih tkiva. Miševi bez IGF-1 receptora umiru u kasnijem periodu razvića, i manifestuju dramatičnu redukcu telesne mase, što potvrđuje jak uticaj ovog receptora. Miševi sa samo jednom funkcionalnom kopijom IGF1R su normalni, ali ispoljavaju ~15% umanjenje telesne mase.. ...
Dolce Elite E5 napravljen je da vas odvede do cilja-fizičkog i geografskog-a da pritom uživate u vožnji. Taj je bicikl savršen uvod u ovaj sport. Odvest će vas na nove razine samopouzdanja na vašem biciklističkom putovanju. U njegovu je srcu naša DSW tehnologija, kojom strateški stavljamo snažnije varove na mjestima gdje su potrebni, pa time dobivamo aluminijski bicikl koji je znatno krući. Točno koliko krući? To je najkrući bicikl od legure koji smo ikad testirali. Inspiriran Rider-First Engineered™ filozofijom, ova rama ima jedinstven down tube, kućište osovine pogona i head tube. Time smo postigli jednake performanse bez obzira na veličinu rame. Kako bi svaki watt snage koji proizvedete bio prenesen na cestu, Elite smo bazirali na ženskoj Endurance Geometriji. Zbog nje ćete imati samopouzdanja na bilo kakvoj podlozi. Ima niži standover kako bi vam olakšali sjedanje na bicikl i silaženje s njega, ali i spuštanje noge. Odabrali smo Shimano Tiagra 10-brzinski mjenjač, brze Axis
Elektroforetskim analizama (Nativna PAGE, izoelektricno fokusiranje, SDS-PAGE) ispitana je mogucnost razlikovanja botanicki razlicitih vrsta meda na temelju njihovog proteinskog, te glikoproteinskog sastava. Detekcijom proteinskih vrpci nakon primjenjenih elektroforetskih metoda, te glikoproteina prenešenih na PVDF membranu nakon SDS-PAGE uoceno je da sve vrste meda pokazuju vrlo slicne proteinske i glikoproteinske elektroforetske profile koji se razlikuju samo po intenzitetu proteinskih vrpci. Prema intenzitetu proteinskih i glikoproteinskih vrpci izdvajaju se med bagrema koji pokazuje najmanji intenzitet, te med kestena, livadni med i bjelogoricna medljika (hrast) sa najjacim intenzitetima. Uklanjanje peludnih zrnaca nije utjecalo na elektroforetski proteinski profil nakon provedene nativne i SDS-PAGE, te izoelektricnog fokusiranja ...
Developing resistant cultivars requires an understanding of the dynamics of the pathogen populations as well as the genetics of host resistance. Bacterial leaf blight (BB), caused by the vascular pathogen Xanthomonas oryzae pv. oryzae, has become one of the most devastating diseases of rice. We demonstrate here the quantitative analyses of responses of near-isogenic lines carrying various BB resistance (R) genes and R-gene combinations against 16 X. oryzae pv. oryzae isolates representing Korean BB pathotypes. The estimated main effects of each R gene against the 16 isolates identified prominent differences in BB pathotypes between Korea and other countries. Three major aspects of our quantitative observations and statistical analysis are (i) strong and broad resistance of xa5; (ii) independent and additive genetic actions of Xa4, xa5, and Xa21 under digenic or trigenic status; and (iii) a strong quantitative complementation effect contributed by the functional alleles of Xa4 and Xa21. We ...
Magnaporthe oryzae causes disease on an array of important crops such as rice, wheat, barley, finger millet, foxtail millet, and wild grasses [1]. M. oryzae has received considerable attention as the causal agent of rice blast disease [2], which leads to 10-30 % loss of harvest per year [3]. M oryzae is categorized into several host-specific subgroups that are pathogenic on a variety of plants that include Oryza spp., Setaria spp., Triticum spp., Avena spp., Eleusine spp. etc. [1, 4, 5]. As with some fungal pathogens M. oryzae reproduction is predominantly asexual. When environmental conditions are conducive, M. oryzae generates an abundance of asexual clones that rapidly colonize its host. The rapid spread of such asexual pathogens poses a significant threat to global food security, human health and biodiversity [6, 7].. Sexual reproduction for M. oryzae is limited to specific geographic regions such as Yunnan, China and India [8-10]. The shift from sexual to asexual reproduction is considered ...
The gene encoding lipase B from Candida antarctica (CalB) was expressed in Pichia pastoris after it was synthesized by the recursive PCR and cloned into the Pichia expression plasmid, pPICZαA. The CalB was successfully secreted in the recombinant P. pastoris strain X-33 with an apparent molecular weight of 34 kDa. For 140 h flask culture, the dry cell weight and the extracellular lipase activity reached at 5.4 g/l and 57.9 U/l toward p-nitrophenyl palmitate, respectively. When we performed the fed-batch fermentation using a methanol feeding strategy for 110 h, the dry cell weight and the extracellular lipase activity were increased to 135.7 g/l and 11,900 U/l; the CalB protein concentration was 1.18 g/l of culture supernatant. The characteristics of CalB recovered from the P. pastoris culture were compared with the commercial form of CalB produced in Aspergillus oryzae. The kinetic constants and specific activity, the effects of activity and stability on temperature and pH, the glycosylation extent,
In this study we identified the L-arabinose-responsive regulator of Pyricularia oryzae that regulates L-arabinose release and catabolism. Previously we identified the Zn2Cys6 transcription factor (TF) AraR that has this role in the Trichocomaceae family (Eurotiales), but is absent in other fungi. Candidate Zn2Cys6 TF genes were selected according to their transcript profiles on L-arabinose. Deletion mutants of these genes were screened for their growth phenotype on L-arabinose. One mutant, named Δara1, was further analyzed. Our analysis demonstrated that Ara1 from P. oryzae is the functional homolog of AraR from A. niger, while sequence analysis did not reveal significant homology between them.
|!--[if gte mso 9]>|xml| |/xml||![endif]--|ISOLATION AND IDENTIFICATION OF RICE PHYLLOSPHERE BACTERIAL ANTAGONISTSAGAINTS Xanthomonas oryzae pv. oryzaeCAUSES OF BACTERIAL LEAF BLIGHT IN RICE (Oryza sativa) Hanna Karunia Agnita, Ari Susilowati, Ratna SetyaningsihDepartment of Biology, Faculty of Mathematics and Natural Sciences,Sebelas Maret University36A Ir. Sutami Street, Kentingan, Surakarta ABSTRACT Bacterial leaf blight (BLB) disease caused by bacteria Xanthomonas oryzae pv. oryzae (Xoo) commonly attacks rice crops and causes agricultural product loss. Phyllosphere bacteria in healthy plant tissue can inhibit the growth of pathogenic bacteria by producing bioactive compounds. This study aimed to isolate rice phyllosphere bacteria, test its inhibition activity to Xoo pathogen, and identify antagonist isolates that can be used as prospective biological control agent of BLB disease.Phyllosphere bacteria was isolated from rice leaves sample in healthy plants among HDB-infected plants in Sukoharjo,
Salmon, salmon meal, peas, sweet potato, pea flour, pea starch, sunflower oil, alfalfa meal, flax seed, calcium carbonate, dried tomato pomace, dicalcium phosphate, salt, potassium chloride, cranberries, minerals (zinc proteinate, iron proteinate, copper proteinate, manganese proteinate, cobalt proteinate, selenium yeast), choline chloride, dried brewers yeast, blueberries, taurine, vitamins (vitamin A acetate, vitamin D3 supplement, vitamin E supplement, niacin, d-calcium pantothenate, thiamine mononitrate, pyridoxine hydrochloride, riboflavin supplement, folic acid, biotin, vitamin B12 supplement), turmeric, dried chicory root, lecithin, lactic acid, glucosamine hydrochloride, garlic, L-ascorbyl-2-polyphosphate (source of vitamin C), chondroitin sulfate, L-carnitine, yucca schidigera extract, calcium iodate, rosemary extract, yeast culture (Saccharomyces cerevisiae), dried Lactobacillus acidophilus fermentation product, dried Enterococcus faecium fermentation product, dried Aspergillus oryzae ...
Scope and method of study: To study the plant cell wall degradation process and changes in overall physiology during the growth of A. nidulans on sorghum stover at proteomic and genomic level, A. nidulans was grown on sorghum stover under solid-state culture conditions for 1, 2, 3, 5, 7 and 14 days. Semi-quantitative extracellular proteome analysis (1-D PAGE LC-MS/MS), whole genome microarray analysis, scanning and transmission electron microscopy, along with qualitative and quantitative analysis of extracellular hydrolytic enzyme activities, and analysis of the breakdown products by enzymes was used to study sorghum cell wall degradation by A. nidulans.Findings and conclusions: Based on analysis of chitin content, A. nidulans grew to be 4-5% of the total biomass in the culture. A hyphal mat developed on the surface of the sorghum by day one and as seen by scanning electron microscopy the hyphae enmeshed the sorghum particles by day 5. A total of 294 extracellular proteins were identified with ...

Aspergillus oryzae RIB40 Genome Snapshot/OverviewAspergillus oryzae RIB40 Genome Snapshot/Overview

This page provides information on the status of the A. oryzae RIB40 genome. Data on this page are updated once a day. All the ... This table displays the current total number of A. oryzae RIB40 gene products that have been annotated to one or more terms in ... To obtain the GO data summarized in these graphs, you may use the GO Slim Mapper, or view and download the file "A_oryzae_RIB40 ... At this time, AspGD does not record sequence variation between the genome reference strain and other strains of A. oryzae RIB40 ...
more infohttp://www.aspergillusgenome.org/cache/A_oryzae_RIB40_genomeSnapshot.html

how to make shio koji 塩麹 - enzyme rich condiment - Shoku Iku Australiahow to make shio koji 塩麹 - enzyme rich condiment - Shoku Iku Australia

Koji is steamed rice that is cultured with special mold/starter, Aspergillus oryzae. This is essential for making amazake, miso ...
more infohttps://shokuikuaustralia.com/how-to-make-shio-koji-%E5%A1%A9%E9%BA%B9-enzyme-rich-condiment/

Aspergillus oryzae - WikipediaAspergillus oryzae - Wikipedia

Aspergillus oryzae, known in English as koji (Japanese: 麹, Hepburn: kōji), is a filamentous fungus (a mold) used in Chinese and ... The genome of A. oryzae is thus one-third larger than that of two related Aspergillus species, the genetics model organism A. ... Resveratrol can be produced from its glucoside piceid through the process of fermentation by A. oryzae. Akira Endo Aspergillus ... Sake Worlds description of koji Aspergillus oryzae genome from the Database of Genomes Analysed at NITE (DOGAN). ...
more infohttps://en.wikipedia.org/wiki/Aspergillus_oryzae

Aspergillus oryzae (Yellow koji mold)Aspergillus oryzae (Yellow koji mold)

Aspergillus oryza. › CBS 466.91. › Eurotium oryzae. › IFO 6215. › NRRL 447. › NRRL MOLD 447. › WB 5032. ... Aspergillus. Strains i. › A1560 / IFO 4177, A1560, IFO 4177. › ATCC 11489 / CBS 125.59 / IMI 52143 / NRRL 695, CBS 125.59, CBS ...
more infohttp://www.uniprot.org/taxonomy/5062

Aspergillus oryzae - microbewikiAspergillus oryzae - microbewiki

Yet unlike in relatives Aspergillus flavus and Aspergillus niger, these genes fail to be expressed in Aspergillus oryzae, ... Close relatives of Aspergillus oryzae, Aspergillus flavus and Aspergillus niger contain syntenic genes from a singular ancestor ... Genome sequencing and analysis of Aspergillus oryzae. Nature 2005. Volume 438. p.1157-1161. [3] "Aspergillus oryzae Final Risk ... Aspergillus oryzae Genome. The full genome of A. oryzae RIB40 contains eight chromosomes and the mitochondrion (which is ...
more infohttps://microbewiki.kenyon.edu/index.php/Aspergillus_oryzae

Aspergillus oryzae (Ahlburg) Cohn ATCC ® 14895™Aspergillus oryzae (Ahlburg) Cohn ATCC ® 14895™

Aspergillus oryzae ATCC ® 14895™ Designation: NRRL 1989 [CBS 134.52, NBIR 2016] Application: produces miso produces soy sauce ... Aspergillus oryzae (Ahlburg) Cohn (ATCC® 14895™) Strain Designations: NRRL 1989 [CBS 134.52, NBIR 2016] / Product Format: ...
more infohttps://www.atcc.org/Products/Cells_and_Microorganisms/By_Focus_Area/Food_Applications/Microbes_for_Food_and_Beverage_Production/14895.aspx

Immobilization of Isolated Lipase From Moldy Copra (Aspergillus Oryzae)Immobilization of Isolated Lipase From Moldy Copra (Aspergillus Oryzae)

... Seniwati Dali,1 A. B. D. Rauf Patong,1 M. Noor ... Aspergillus Oryzae)," E-Journal of Chemistry, vol. 8, no. 2, pp. 896-902, 2011. https://doi.org/10.1155/2011/608075. ...
more infohttps://www.hindawi.com/journals/jchem/2011/608075/cta/

KEGG PATHWAY: Citrate cycle (TCA cycle) - Aspergillus oryzaeKEGG PATHWAY: Citrate cycle (TCA cycle) - Aspergillus oryzae

Citrate cycle (TCA cycle) - Aspergillus oryzae [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
more infohttp://www.genome.jp/kegg-bin/show_pathway?aor00020

hapC - HAPC - Aspergillus oryzae (Yellow koji mold) - hapC gene & proteinhapC - HAPC - Aspergillus oryzae (Yellow koji mold) - hapC gene & protein

Aspergillus rambellii. Aspergillus ochraceoroseus. Aspergillus oryzae (strain 3.042) (Yellow koji mold). Aspergillus flavus ( ... Aspergillus oryzae (strain 3.042) (Yellow koji mold). Aspergillus flavus (strain ATCC MYA-384 / AF70). Aspergillus parasiticus ... Aspergillus rambellii. Aspergillus ochraceoroseus. Aspergillus carbonarius (strain ITEM 5010). Aspergillus nomius NRRL 13137. ... Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold). Aspergillus bombycis. 215. UniRef90_A0A0L1J748. Cluster: ...
more infohttp://www.uniprot.org/uniprot/O59848

Search results for: Aspergillus oryzaeSearch results for: 'Aspergillus oryzae'

Search results for Aspergillus oryzae. A partial match was found for aspergillus oryzae Please try browsing our store to find ... Aspergillus fumigatus IgG ELISA Kit (Human) (OKNA00103) Protein Name:Aspergillus fumigatus IgG ... Aspergillus fumigatus IgM ELISA Kit (Human) (OKNA00104) Protein Name:Aspergillus fumigatus IgM ... Rabbit ANTI ASPERGILLUS NIGER GLUCOSE OXIDASE Antibody (OASA06989) Protein Name:Glucose oxidase ...
more infohttps://www.avivasysbio.com/en/catalogsearch/result/?q=Aspergillus%20oryzae

Survey of the transcriptome of Aspergillus oryzae via massively parallel mRNA sequencing.  - PubMed - NCBISurvey of the transcriptome of Aspergillus oryzae via massively parallel mRNA sequencing. - PubMed - NCBI

Survey of the transcriptome of Aspergillus oryzae via massively parallel mRNA sequencing.. Wang B1, Guo G, Wang C, Lin Y, Wang ... Aspergillus oryzae, an important filamentous fungus used in food fermentation and the enzyme industry, has been shown through ... AS events in the A. oryzae transcriptome. (A) The schematic depicts the six primary types of AS events in the A. oryzae ... Survey of the transcriptome of Aspergillus oryzae via massively parallel mRNA sequencing ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/20392818?dopt=Abstract

Cloning and nucleotide sequence of the calmodulin-encoding gene (cmdA) from Aspergillus oryzae.  - PubMed - NCBICloning and nucleotide sequence of the calmodulin-encoding gene (cmdA) from Aspergillus oryzae. - PubMed - NCBI

Cloning and nucleotide sequence of the calmodulin-encoding gene (cmdA) from Aspergillus oryzae.. Yasui K1, Kitamoto K, Gomi K, ... A cDNA and genomic gene encoding calmodulin were isolated from Aspergillus oryzae using a part of the calmodulin gene from A. ... The cDNA encoding A. oryzae calmodulin was expressed under the control of the GAL1 promoter in the calmodulin null mutant (cmd1 ... oryzae. The nucleotide sequence analysis showed that the gene consists of five introns and six exons. Although the nucleotide ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/7549095?dopt=Abstract

Glycosylation analysis of recombinant neutral protease I from Aspergillus oryzae expressed in Pichia pastoris | SpringerLinkGlycosylation analysis of recombinant neutral protease I from Aspergillus oryzae expressed in Pichia pastoris | SpringerLink

Neutral protease I from Aspergillus oryzae 3.042 was expressed in Pichia pastoris and its N-glycosylation properties were ... Machida M, Asai K, Sano M et al (2005) Genome sequencing and analysis of Aspergillus oryzae. Nature 438:1157-1161PubMedCrossRef ... Aspergillus oryzae N-glycosylation Nano LC-MS/MS Pichia pastoris Protease Recombinant neutral protease I ... Tatsumi H, Murakami S, Tsuji R et al (1991) Cloning and expression in yeast of a cDNA clone encoding Aspergillus oryzae neutral ...
more infohttps://link.springer.com/article/10.1007/s10529-013-1314-3

Investigation of malic acid production in Aspergillus oryzae under nitrogen starvation conditions. | Sigma-AldrichInvestigation of malic acid production in Aspergillus oryzae under nitrogen starvation conditions. | Sigma-Aldrich

Investigation of malic acid production in Aspergillus oryzae under nitrogen starvation conditions.. [Christoph Knuf, Intawat ... As A. oryzae is a very close relative or even an ecotype of A. flavus, it is likely that its high malic acid production ... Natural high-capacity malic acid producers like the malic acid producer Aspergillus flavus have so far been disqualified ...
more infohttps://www.sigmaaldrich.com/catalog/papers/23892740

Sequence Similarity 









- 1IZE: Crystal structure of Aspergillus oryzae Aspartic proteinase complexed with pepstatin...Sequence Similarity - 1IZE: Crystal structure of Aspergillus oryzae Aspartic proteinase complexed with pepstatin...

Crystal structures of Aspergillus oryzae aspartic proteinase and its complex with an inhibitor pepstatin at 1.9A resolution. ...
more infohttps://www.rcsb.org/pdb/explore/sequenceCluster.do?structureId=1IZE

Analysis of the histone deacetylases disruptants of Aspergillus oryzae | Aspergillus & Aspergillosis WebsiteAnalysis of the histone deacetylases disruptants of Aspergillus oryzae | Aspergillus & Aspergillosis Website

Aspergillus oryzae is ubiquitous filamentous fungi in nature and has been used in a number of industries such as Japanese ... Analysis of the histone deacetylases disruptants of Aspergillus oryzae. Moriyuki Kawauchi[1] Mika Nishiura[1] Kazuhiro Iwashita ... Please note: The Aspergillus Website does not accept advertisments. , all content is published on merit. BB.com. Site Sponsors ... Aspergillus Website Newsletters. Our most recent newsletters (since August 2018) can be found here. ...
more infohttps://www.aspergillus.org.uk/content/analysis-histone-deacetylases-disruptants-aspergillus-oryzae

PRIME PubMed | Growth, ruminal measurements, and health characteristics of Holstein bull calves fed an Aspergillus oryzae...PRIME PubMed | Growth, ruminal measurements, and health characteristics of Holstein bull calves fed an Aspergillus oryzae...

... and health characteristics of Holstein bull calves fed an Aspergillus oryzae fermentation extrac were found in PRIME PubMed. ... Animal FeedAnimalsAspergillus oryzaeBody WeightCattleDietFecesFermentationHydrogen-Ion ConcentrationMaleMilk SubstitutesRumen ... VL - 98 IS - 9 N2 - A fermentation extract of the fungus Aspergillus oryzae can be used as a prebiotic. The objective was to ... A fermentation extract of the fungus Aspergillus oryzae can be used as a prebiotic. The objective was to determine if dietary ...
more infohttps://www.unboundmedicine.com/medline/citation/26142841/Growth_ruminal_measurements_and_health_characteristics_of_Holstein_bull_calves_fed_an_Aspergillus_oryzae_fermentation_extract_

The influence of nitrogen sources on the α-amylase productivity of Aspergillus oryzae in continuous cultures | SpringerLinkThe influence of nitrogen sources on the α-amylase productivity of Aspergillus oryzae in continuous cultures | SpringerLink

The influence of the nitrogen source on the α-amylase productivity of Aspergillus oryzaewas quantified in continuous ... The influence of the nitrogen source on the α-amylase productivity of Aspergillus oryzae was quantified in continuous ... The influence of nitrogen sources on the α-amylase productivity of Aspergillus oryzae in continuous cultures. ...
more infohttps://link.springer.com/article/10.1007%2Fs002530050021

Catalysts | Free Full-Text | Cloning, Expression and Characterization of a Novel Fructosyltransferase from Aspergillus oryzae...Catalysts | Free Full-Text | Cloning, Expression and Characterization of a Novel Fructosyltransferase from Aspergillus oryzae...

The recombinant A. oryzae ZZ-01 fructosyltransferases (r-AoFT) was purified to homogeneity after Ni-NTA affinity and Superdex- ... A 1521 bp gene encoding for a novel fructosyltransferase from Aspergillus oryzae ZZ-01 (AoFT) has been amplified by RACE and ... Aspergillus oryzae; gene cloning; site-directed mutagenesis; characterization fructosyltransferase; Aspergillus oryzae; gene ... A 1521 bp gene encoding for a novel fructosyltransferase from Aspergillus oryzae ZZ-01 (AoFT) has been amplified by RACE and ...
more infohttps://www.mdpi.com/2073-4344/6/5/67

RCSB PDB - 3C97: Crystal structure of the response regulator receiver domain of a signal transduction histidine kinase from...RCSB PDB - 3C97: Crystal structure of the response regulator receiver domain of a signal transduction histidine kinase from...

Crystal structure of the response regulator receiver domain of a signal transduction histidine kinase from Aspergillus oryzae ... Aspergillus oryzae (strain ATCC 42149 / RIB 40). Mutation(s): 0 Find proteins for Q2U940 (Aspergillus oryzae (strain ATCC 42149 ... Crystal structure of the response regulator receiver domain of a signal transduction histidine kinase from Aspergillus oryzae. ... Crystal structure of the response regulator receiver domain of a signal transduction histidine kinase from Aspergillus oryzae. ...
more infohttps://www.rcsb.org/structure/3c97

Identification and characterization of novel type III polyketide synthases in Aspergillus oryzae.  | Aspergillus &...Identification and characterization of novel type III polyketide synthases in Aspergillus oryzae. | Aspergillus &...

Aspergillus oryzae. While A. oryzae genome data mining revealed 4 putative CHS-like sequences (csyA, csyB, csyC and csyD), ... Identification and characterization of novel type III polyketide synthases in Aspergillus oryzae. Yasuyo Seshime1, Praveen Rao ... Interestingly, the other species of Aspergilli (Aspergillus nidulans, Aspergillus fumigatus) lacked these genes. Significantly ... Please note: The Aspergillus Website does not accept advertisments. , all content is published on merit. BB.com. Site Sponsors ...
more infohttps://www.aspergillus.org.uk/content/identification-and-characterization-novel-type-iii-polyketide-synthases-aspergillus-oryzae

Catalysts  | Free Full-Text | Exploiting the Versatility of Aminated Supports Activated with Glutaraldehyde to Immobilize β...Catalysts | Free Full-Text | Exploiting the Versatility of Aminated Supports Activated with Glutaraldehyde to Immobilize β...

The enzyme β-galactosidase from Aspergillus oryzae has been immobilized in aminated (MANAE)-agarose beads via glutaraldehyde ... The enzyme β-galactosidase (β-gal) from Aspergillus oryzae is a glycosylated enzyme whose structure has been recently resolved ... Vera, C.; Guerrero, C.; Illanes, A.; Conejeros, R. Fed-batch synthesis of galacto-oligosaccharides with Aspergillus oryzae β- ... The enzyme β-galactosidase from Aspergillus oryzae has been immobilized in aminated (MANAE)-agarose beads via glutaraldehyde ...
more infohttp://www.mdpi.com/2073-4344/7/9/250/htm

Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae |...Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae |...

Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae. ... Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae ... Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae ... Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae ...
more infohttp://www.jimmunol.org/content/186/7/3966/tab-article-info

Cassava Biomass Transformation by Aspergillus oryzae, Vol. 8, No. 9 (2016) | Journal of Agricultural ScienceCassava Biomass Transformation by Aspergillus oryzae, Vol. 8, No. 9 (2016) | Journal of Agricultural Science

Aspergillus oryzae was used for liquefaction and saccharification of cassava starch. It was obtained wort with 13.03 °Brix and ... Aspergillus oryzae was used for liquefaction and saccharification of cassava starch. It was obtained wort with 13.03 °Brix and ...
more infohttp://www.ccsenet.org/journal/index.php/jas/article/view/58085/33340

In-vitro Biosorption of Lead and Zinc by using living Biomass of Aspergillus oryzae - H. S. Ravikumar Patil, H.K. Makari, H....In-vitro Biosorption of Lead and Zinc by using living Biomass of Aspergillus oryzae - H. S. Ravikumar Patil, H.K. Makari, H....

In-vitro Biosorption of Lead and Zinc by using living Biomass of Aspergillus oryzae. By H. S. Ravikumar Patil, H. K. Makari and ... Some biomasses of fungi types are very effective in accumulating heavy metals, such as Aspergillus niger, Aspergillus terreus, ... It was also observed that removal efficiency of Aspergillus oryzae decreases with increasing concentrations. ... Aspergillus oryzae showed the maximum percentage removal of Pb and Zn at 20 ppm concentration and minimum adsorption at 100 ppm ...
more infohttps://eco-web.com/edi/070922.html
  • Ke Y, Huang WQ, Li JZ et al (2012) Enzymatic characteristics of a recombinant neutral protease I (rNpI) from Aspergillus oryzae expressed in Pichia pastoris . (springer.com)
  • The recombinant A. oryzae ZZ-01 fructosyltransferases (r- Ao FT) was purified to homogeneity after Ni-NTA affinity and Superdex-200 gel filtration chromatography. (mdpi.com)
  • High purity exo-1,3-beta-Glucanase (Aspergillus oryzae) (Recombinant) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (creative-enzymes.com)
  • The A. oryzae -specific sequence codes for metabolite synthesis and specific gene expansion for secreting hydrolytic enzymes when used in SSF, or solid-state fermentation, which makes it such an effective microbe in fermentation processes. (kenyon.edu)
  • Because A. oryzae has been domesticated, it is possible that gene expansion is due to horizontal gene transfer, as is seen in A. oryzae -specific genes, which use clonal lines to transfer chromosomes. (kenyon.edu)
  • Y16967 Aspergillus oryzae aflR gene. (atcc.org)
  • The transcriptional activity of the A. oryzae AF biosynthesis gene cluster detected by RNA-Seq. (nih.gov)
  • Cloning and nucleotide sequence of the calmodulin-encoding gene (cmdA) from Aspergillus oryzae. (nih.gov)
  • The gene was in a 3.4-kb SphI fragment and Southern-blot analysis of genomic DNA suggested the existence of a single copy of the calmodulin gene in A. oryzae. (nih.gov)
  • The cDNA encoding A. oryzae calmodulin was expressed under the control of the GAL1 promoter in the calmodulin null mutant (cmd1) of yeast, Saccharomyces cerevisiae, and could function as a calmodulin gene. (nih.gov)
  • A 1521 bp gene encoding for a novel fructosyltransferase from Aspergillus oryzae ZZ-01 ( Ao FT) has been amplified by RACE and TAIL PCR, and functionally overexpressed in Escherichia coli BL 21-CodonPlus (DE3)-RIL. (mdpi.com)
  • These chains aid in the development of the Aspergillus cell membrane and the enzyme storage vesicles. (kenyon.edu)
  • The enzyme β-galactosidase from Aspergillus oryzae has been immobilized in aminated (MANAE)-agarose beads via glutaraldehyde chemistry using different strategies. (mdpi.com)
  • In view of the ability of A. oryzae to accommodate a high concentration of GA and achieve a high removal rate, as well as the significantly different enzyme activities involved in GA degradation and the underlying mechanisms between the two fungal strains, A. oryzae is proven to be a superior strain for the degradation of DOP. (iwaponline.com)
  • Aspergillus oryzae, known in English as koji (Japanese: 麹, Hepburn: kōji), is a filamentous fungus (a mold) used in Chinese and other East Asian cuisines to ferment soybeans for making soy sauce and fermented bean paste, and also to saccharify rice, other grains, and potatoes in the making of alcoholic beverages such as huangjiu, sake, makgeolli, and shōchū. (wikipedia.org)
  • AU - Yohe,T T, AU - O'Diam,K M, AU - Daniels,K M, Y1 - 2015/07/02/ PY - 2015/01/05/received PY - 2015/05/26/accepted PY - 2015/7/6/entrez PY - 2015/7/6/pubmed PY - 2016/6/9/medline KW - Aspergillus oryzae KW - dairy calf KW - growth KW - prebiotic SP - 6163 EP - 75 JF - Journal of dairy science JO - J. Dairy Sci. (unboundmedicine.com)
  • Aspergillus oryzae is a filamentous fungus, or mold, that is used in East Asian (particularly Japanese and Chinese) food production, such as in soybean fermentation. (kenyon.edu)
  • Aspergillus oryzae is ubiquitous filamentous fungi in nature and has been used in a number of industries such as Japanese traditional fermented foods and pharmaceutical products. (aspergillus.org.uk)
  • Bioremediation for elimination of heavy metals gaining much importance through microorganisms .This study was aimed to in-vitro elimination of lead and zinc by using of filamentous fungi A spergillus oryzae . (eco-web.com)
  • Tatsumi H, Murakami S, Tsuji R et al (1991) Cloning and expression in yeast of a cDNA clone encoding Aspergillus oryzae neutral protease II, a unique metalloprotease. (springer.com)
  • Natural high-capacity malic acid producers like the malic acid producer Aspergillus flavus have so far been disqualified because of special growth requirements or the production of mycotoxins. (sigmaaldrich.com)
  • As A. oryzae is a very close relative or even an ecotype of A. flavus, it is likely that its high malic acid production capabilities with a generally regarded as safe (GRAS) status may be combined with already existing large-scale fermentation experience. (sigmaaldrich.com)
  • Bamforth (2005) places the probable origin of true sake (which is made from rice, water, and kōji mold (麹, Aspergillus oryzae) in the Nara period (710-794). (wikipedia.org)
  • In this study we report the heterologous expression of Aspergillus oryzae β-xylosidase (XylA) in Pichia pastoris under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter. (nottingham.ac.uk)
  • Wei T, Huang S, Zang J, Jia C, Mao D. Cloning, Expression and Characterization of a Novel Fructosyltransferase from Aspergillus oryzae ZZ-01 for the Synthesis of Sucrose 6-Acetate. (mdpi.com)
  • Aspergillus oryzae was used for liquefaction and saccharification of cassava starch. (ccsenet.org)
  • Most other enzymes found in A. oryzae grow at a temperature of around 30-35 degrees Celsius. (kenyon.edu)
  • These metabolites grant A. oryzae the ability to modify themselves according to their current environment--they are able to increase or decrease their fitness to allow optimum metabolic efficiency. (kenyon.edu)
  • Members of the Aspergillus genus are distinct from other microbes due to the fact that they utilize both a primary and secondary metabolic system. (kenyon.edu)
  • The secondary metabolism utilizes acidic compounds to suppress metabolic pathways, which allows A. oryzae to produce secondary metabolites. (kenyon.edu)
  • Here, we applied direct high-throughput paired-end RNA-sequencing (RNA-Seq) to the transcriptome of A. oryzae under four different culture conditions. (nih.gov)
  • A partial match was found for aspergillus oryzae Please try browsing our store to find what you want. (avivasysbio.com)
  • The activity of laccase and lignin peroxidase excreted by A. oryzae was higher than that by P. chrysosporium in the presence of GA. Based on the results of high-performance liquid chromatography-electrospray ionization-mass spectrometry, three relevant intermediate metabolites were determined as progallin A, methyl gallate, and pyrogallic acid, implying that A. oryzae could not degrade GA unless the carboxyl in the molecule was protected or removed. (iwaponline.com)
  • This study has indicated that HDACs play important roles in the growth and stress adaptation of A. oryzae. (aspergillus.org.uk)
  • This graph shows the total number of publications written about "Aspergillus oryzae" by people in this website by year, and whether "Aspergillus oryzae" was a major or minor topic of these publications. (ucdenver.edu)
  • Dietary inclusion (2 g/d) of an extract of A. oryzae did not affect calf growth, intake, and ruminal or health measurements. (unboundmedicine.com)
  • Significantly, A. oryzae alone contained higher number of putative chalcone synthase homologues in comparison to Neurospora crassa (1), Fusarium graminearum (1), Magnaporthe grisea (2) and Phanerochaete chrysosporium (3). (aspergillus.org.uk)
  • Aspergillus oryzae showed the maximum percentage removal of Pb and Zn at 20 ppm concentration and minimum adsorption at 100 ppm. (eco-web.com)
  • Below are the most recent publications written about "Aspergillus oryzae" by people in Profiles. (ucdenver.edu)