An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.
A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.
A species of imperfect fungi from which the antibiotic fumigatin is obtained. Its spores may cause respiratory infection in birds and mammals.
A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.
A species of imperfect fungi which grows on peanuts and other plants and produces the carcinogenic substance aflatoxin. It is also used in the production of the antibiotic flavicin.
An enzyme that catalyzes the hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of polysaccharide chains with the release of beta-glucose. It is also able to hydrolyze 1,6-alpha-glucosidic bonds when the next bond in sequence is 1,4.
Infections with fungi of the genus ASPERGILLUS.
An enzyme that catalyzes the conversion of myo-inositol hexakisphosphate and water to 1L-myo-inositol 1,2,3,4,5-pentakisphosphate and orthophosphate. EC 3.1.3.26.
Proteins found in any species of fungus.
Reproductive bodies produced by fungi.
A cell wall-degrading enzyme found in microorganisms and higher plants. It catalyzes the random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. EC 3.2.1.15.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.
A key intermediate in metabolism. It is an acid compound found in citrus fruits. The salts of citric acid (citrates) can be used as anticoagulants due to their calcium chelating ability.
A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.
The functional hereditary units of FUNGI.
Deoxyribonucleic acid that makes up the genetic material of fungi.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.
A group of enzymes that catalyze the hydrolysis of alpha- or beta-xylosidic linkages. EC 3.2.1.8 catalyzes the endo-hydrolysis of 1,4-beta-D-xylosidic linkages; EC 3.2.1.32 catalyzes the endo-hydrolysis of 1,3-beta-D-xylosidic linkages; EC 3.2.1.37 catalyzes the exo-hydrolysis of 1,4-beta-D-linkages from the non-reducing termini of xylans; and EC 3.2.1.72 catalyzes the exo-hydrolysis of 1,3-beta-D-linkages from the non-reducing termini of xylans. Other xylosidases have been identified that catalyze the hydrolysis of alpha-xylosidic bonds.
An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A cyclic polypeptide antibiotic isolated from culture filtrates of Bacillus subtilis that acts as an antifungal agent.
The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.
Polysaccharides consisting of xylose units.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Microscopic threadlike filaments in FUNGI that are filled with a layer of protoplasm. Collectively, the hyphae make up the MYCELIUM.
The body of a fungus which is made up of HYPHAE.
Furano-furano-benzopyrans that are produced by ASPERGILLUS from STERIGMATOCYSTIN. They are structurally related to COUMARINS and easily oxidized to an epoxide form to become ALKYLATING AGENTS. Members of the group include AFLATOXIN B1; aflatoxin B2, aflatoxin G1, aflatoxin G2; AFLATOXIN M1; and aflatoxin M2.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Isocoumarins found in ASPERGILLUS OCHRACEUS and other FUNGI. Ochratoxin contaminated FOOD has been responsible for cases of FOODBORNE DISEASES.
An imperfect fungus that produces ochratoxins and contaminates EDIBLE GRAIN and coffee beans.
Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.
Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Enzymes which catalyze the hydrolysis of carboxylic acid esters with the formation of an alcohol and a carboxylic acid anion.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.
A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.
A dextrodisaccharide from malt and starch. It is used as a sweetening agent and fermentable intermediate in brewing. (Grant & Hackh's Chemical Dictionary, 5th ed)
Substances capable of inhibiting, retarding or arresting the process of fermentation, acidification or other deterioration of foods.
High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.
Infections of the respiratory tract with fungi of the genus ASPERGILLUS. Infections may result in allergic reaction (ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS), colonization in pulmonary cavities as fungus balls (MYCETOMA), or lead to invasion of the lung parenchyma (INVASIVE PULMONARY ASPERGILLOSIS).
A genus of zygomycetous fungi of the family Mucoraceae, order MUCORALES, a common saprophyte and facultative parasite of mature fruits and vegetables. It may cause cerebral mycoses in diabetes and cutaneous infection in severely burned patients.
A triazole antifungal agent that inhibits cytochrome P-450-dependent enzymes required for ERGOSTEROL synthesis.
Hypersensitivity reaction (ALLERGIC REACTION) to fungus ASPERGILLUS in an individual with long-standing BRONCHIAL ASTHMA. It is characterized by pulmonary infiltrates, EOSINOPHILIA, elevated serum IMMUNOGLOBULIN E, and skin reactivity to Aspergillus antigen.
An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.
A carcinogenic mycotoxin produced in high yields by strains of the common molds, Aspergillus versicolor, A. nidulans, and an unidentified species of Bipolaris. It causes necrosis of the liver and kidney and has an inhibitory effect on orotic acid incorporation into nuclear RNA.
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
The ability of fungi to resist or to become tolerant to chemotherapeutic agents, antifungal agents, or antibiotics. This resistance may be acquired through gene mutation.
A disaccharide consisting of two glucose units in an alpha (1-6) glycosidic linkage.
Toxic compounds produced by FUNGI.
The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Pulmonary diseases caused by fungal infections, usually through hematogenous spread.
An enzyme that catalyzes the hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. The enzyme plays a role in the lysosomal degradation of the N-glycosylprotein glycans. Defects in the lysosomal form of the enzyme in humans result in a buildup of mannoside intermediate metabolites and the disease BETA-MANNOSIDOSIS.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Polysaccharides consisting of mannose units.
A mitosporic fungal genus frequently found in soil and on wood. It is sometimes used for controlling pathogenic fungi. Its teleomorph is HYPOCREA.
A xylosidase that catalyses the random hydrolysis of 1,3-beta-D-xylosidic linkages in 1,3-beta-D-xylans.
The rate dynamics in chemical or physical systems.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
The process of cleaving a chemical compound by the addition of a molecule of water.
Mold and yeast inhibitor. Used as a fungistatic agent for foods, especially cheeses.
The productive enterprises concerned with food processing.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
An imperfect fungus present on most agricultural seeds and often responsible for the spoilage of seeds in bulk storage. It is also used in the production of fermented food or drink, especially in Japan.
Macrolide antifungal antibiotic produced by Streptomyces nodosus obtained from soil of the Orinoco river region of Venezuela.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Lung infections with the invasive forms of ASPERGILLUS, usually after surgery, transplantation, prolonged NEUTROPENIA or treatment with high-doses of CORTICOSTEROIDS. Invasive pulmonary aspergillosis can progress to CHRONIC NECROTIZING PULMONARY ASPERGILLOSIS or hematogenous spread to other organs.
Enzymes that catalyze the exohydrolysis of 1,4-alpha-glucosidic linkages with release of alpha-glucose. Deficiency of alpha-1,4-glucosidase may cause GLYCOGEN STORAGE DISEASE TYPE II.

Purification and characterization of methylamine oxidase induced in Aspergillus niger AKU 3302. (1/718)

Crude extract of Aspergillus niger AKU 3302 mycelia incubated with methylamine showed a single amine oxidase activity band in a developed polyacrylamide gel that weakly cross-reacted with the antibody against a copper/topa quinone-containing amine oxidase (AO-II) from the same strain induced by n-butylamine. Since the organism cannot grow on methylamine and the already known quinoprotein amine oxidases of the organism cannot catalyze oxidation of methylamine, the organism was forced to produce another enzyme that could oxidize methylamine when the mycelia were incubated with methylamine. The enzyme was separated and purified from the already known two quinoprotein amine oxidases formed in the same mycelia. The purified enzyme showed a sharp symmetric sedimentation peak in analytical ultracentrifugation showing S20,w0 of 6.5s. The molecular mass of 133 kDa estimated by gel chromatography and 66.6 kDa found by SDS-PAGE confirmed the dimeric structure of the enzyme. The purified enzyme was pink in color with an absorption maximum at 494 nm. The enzyme readily oxidized methylamine, n-hexylamine, and n-butylamine, but not benzylamine, histamine, or tyramine, favorite substrates for the already known two quinoprotein amine oxidases. Inactivation by carbonyl reagents and copper chelators suggested the presence of a copper/topa quinone cofactor. Spectrophotometric titration by p-nitrophenylhydrazine showed one reactive carbonyl group per subunit and redox-cyclic quinone staining confirmed the presence of a quinone cofactor. pH-dependent shift of the absorption spectrum of the enzyme-p-nitrophenylhydrazone (469 nm at neutral to 577 nm at alkaline pH) supported the identity of the cofactor with topaquinone. Nothern blot analysis indicated that the methylamine oxidase encoding gene is largely different from the already known amine oxidase in the organism.  (+info)

Citric acid production from xylan and xylan hydrolysate by semi-solid culture of Aspergillus niger. (2/718)

Citric acid production from xylan and xylan hydrolysate was done by Aspergillus niger Yang no. 2 cultivated in a semi-solid culture using bagasse as a carrier. Yang no. 2 produced 72.4 g/l and 52.6 g/l of citric acid in 5 d from 140 g/l of xylose and arabinose, respectively. Yang no. 2 produced 51.6 g/l of citric acid in 3 d from a concentrated xylan hydrolysate prepared by cellulase treatment, containing 100 g/l of reducing sugars. Moreover, Yang no. 2 directly produced 39.6 g/l of citric acid maximally in 3 d from 140 g/l of xylan.  (+info)

Production of specific monoclonal antibodies to Aspergillus species and their use in immunohistochemical identification of aspergillosis. (3/718)

Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining.  (+info)

Kinetic characterization of Aspergillus niger N400 endopolygalacturonases I, II and C. (4/718)

Endopolygalacturonases I, II and C isolated from recombinant Aspergillus niger strains were characterized with respect to pH optimum, activity on polygalacturonic acid and mode of action and kinetics on oligogalacturonates of different chain length (n = 3-7). Apparent Vmax values using polygalacturonate as a substrate at the pH optimum, pH 4.1, were calculated as 13.8 mukat.mg-1, 36.5 mukat.mg-1 and 415 nkat.mg-1 for endopolygalacturonases I, II and C, respectively. K(m) values were < 0.15 mg.mL-1 for all three enzymes. Product progression analysis using polygalacturonate as a substrate revealed a random cleavage pattern for all three enzymes and suggested processive behavior for endopolygalacturonases I and C. This result was confirmed by analysis of the mode of action using oligogalacturonates. Processivity was observed when the degree of polymerization of the substrate exceeded 5 or 6 for endopolygalacturonase I and endopolygalacturonase C, respectively. The bond-cleavage frequencies obtained for the hydrolysis of the oligogalacturonates were used to assess subsite maps. The maps indicate that the minimum number of subsites is seven for all three enzymes. Using pectins of various degrees of esterification, it was shown that endopolygalacturonase II is the most sensitive to the presence of methyl esters. Like endopolygalacturonase II, endopolygalacturonases I, C and E, which was also included in this part of the study, preferred the non-esterified pectate. Additional differences in substrate specificity were revealed by analysis of the reaction products of hydrolysis of a mixture of pectate lyase-generated delta 4,5-unsaturated oligogalacturonates of degree of polymerization 4-8. Whereas endopolygalacturonase I showed a strong preference for generating the delta 4,5-unsaturated dimer, with endopolygalacturonase II the delta 4,5-unsaturated trimer accumulated, indicating further differences in substrate specificity. For endopolygalacturonases C and E both the delta 4,5-unsaturated dimer and trimer were observed, although in different ratios.  (+info)

Possibility for discriminating between two representative non two-state thermal unfolding models of proteins by DSC. (5/718)

Possible differences between two representative non two-state thermal unfolding mechanisms of protein are discussed concerning differential scanning calorimetry. Numerical simulations showed that, by DSC measurement, it is hard to discriminate between the independent model, which assumes independent unfolding domains in a protein, and the sequential model, which assumes intermediate(s) between native and denatured states, especially when values of molecular weight, denaturation enthalpy, and difference in denaturation temperature of each denaturation process are large. DSC curve analysis of Aspergillus niger glucoamylase based on these two models gave essentially the same thermodynamic parameters.  (+info)

Pseudoepidemic of Aspergillus niger infections traced to specimen contamination in the microbiology laboratory. (6/718)

We report a pseudo-outbreak of Aspergillus niger that followed building construction in our clinical microbiology laboratory. Because outbreaks of invasive aspergillosis have been linked to hospital construction, strategies to minimize dust in patient care areas are common practice. We illustrate that the impact of false-positive cultures on patient care should compel laboratories to prevent specimen contamination during construction.  (+info)

Oxidation of medium-chain acyl-CoA esters by extracts of Aspergillus niger: enzymology and characterization of intermediates by HPLC. (7/718)

The activities of beta-oxidation enzymes were measured in extracts of glucose- and triolein-grown cells of Aspergillus niger. Growth on triolein stimulated increased enzyme activity, especially for acyl-CoA dehydrogenase. No acyl-CoA oxidase activity was detected. HPLC analysis after incubation of triolein-grown cell extracts with decanoyl-CoA showed that beta-oxidation was limited to one cycle. Octanoyl-CoA accumulated as the decanoyl-CoA was oxidized. Beta-oxidation enzymes in isolated mitochondrial fractions were also studied. The results are discussed in the context of methyl ketone production by fungi.  (+info)

Biotransformation of (1-phenyl)ethyl hydroperoxide with Aspergillus niger: a model study on enzyme selectivity and on the induction of peroxidase activity. (8/718)

The biocatalytic enantioselective reduction of (1-phenyl)ethyl hydroperoxide (1) by the fungus Aspergillus niger to the corresponding alcohol 2 involves a multi-enzyme biotransformation of the hydroperoxide 1, as revealed by the change in the enantioselectivity as a function of incubation times. This unusual behavior is not exhibited by other fungi and seems to be restricted to A. niger. Furthermore, the peroxidase and other oxidoreductase activities of A. niger depend on the availability of metal ions such as Fe2+, Mn2+ and Zn2+ in the growth medium, since the addition of Fe2+ ions substantially (threefold) increases the enantioselectivity, whereas addition of Mn2+ and Zn2+ ions decreases it. Finally, the cold shock (4 degrees C) significantly enhances the reduction of the hydroperoxide by the microorganism A. niger.  (+info)

The cDNA encoding for catalytic subunit of bovine enterokinase (EK(L)), to which the sequence for Kex2 protease cleavage site was inserted, was expressed in the protease deficient filamentous fungus Aspergillus niger AB1.13. Fungal transformants were obtained in which expression of the glucoamylase …
Here, we have synthesized the silver nanoparticles (AgNPs) by using the soil fungus Aspergillus niger 2587. The results recorded from UV-vis spectrophotometer and transmission electron microscopy (TEM) support the biosynthesis and characterization of AgNPs. The synthesized silver nanoparticles have also been tested against the larvae and pupae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti. The efficacy test was performed at different concentrations for a period of different hours by the probit analysis. The larvae of Cx. quinquefasciatus have shown the 100% mortality to the synthesized AgNPs after 1 h of exposure, while the larvae of An. stephensi and Ae. aegypti were found less susceptible to the synthesized AgNPs. The pupa of Ae. aegypti has shown the efficacy LC50 4, LC90 12 and LC99 19 ppm after 2 h of exposure of the synthesized AgNPs, while, the pupae of Cx. quinquefasciatus and An. stephensi were found less susceptible to the synthesized AgNPs. By this approach, it is
The filamentous fungus Aspergillus niger was transformed with the hepatitis B virus S gene encoding the major viral envelope protein under control of the constitutive A. nidulans glyceraldehyde-3-phosphate dehydrogenase (gpdA) promoter. Approximately seven copies of the expression cassette were integrated on the genome, resulting in high-level transcription of the S gene. Production of the 24-kDa S protein and a 48-kDa S protein dimer in the membrane-associated protein fraction of the recombinant A. niger strain was shown through Western analysis. Electron microscopy of partially purified recombinant S protein revealed the formation of spherical pseudoviral particles with a diameter of 22 nm. The production level of hepatitis B pseudoviral particles was estimated to be 0.4 mg/1 culture, which compares favourably with the reported levels initially obtained in yeast, indicating the potential of the Aspergillus expression system as an alternative, cost-effective vaccine production system.. ...
Aspergillus niger ATCC ® 1015D-2™ Designation: Genomic DNA from Aspergillus niger strain 3528.7 [ATCC ® 1015™] Application:
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Abstract Background The filamentous fungus Aspergillus niger is well-known as a producer of primary metabolites and extracellular proteins. For example, glucoamylase is the most efficiently secreted protein of Aspergillus niger, thus the homologous glucoamylase (glaA) promoter as well as the glaA signal sequence are widely used for heterologous protein production. Xylose is known to strongly repress glaA expression while maltose is a potent inducer of glaA promoter controlled genes. For a more profound understanding of A. niger physiology, a comprehensive analysis of the intra- and extracellular proteome of Aspergillus niger AB1.13 growing on defined medium with xylose or maltose as carbon substrate was carried out using 2-D gel electrophoresis/Maldi-ToF and nano-HPLC MS/MS. Results The intracellular proteome of A. niger growing either on xylose or maltose in well-aerated controlled bioreactor cultures revealed striking similarities. In both cultures the most abundant intracellular protein was ...
A recombinant Aspergillus niger strain expressing the Hypocrea jecorina endoglucanase Cel7B was grown on spent hydrolysates (stillage) from sugarcane bagasse and spruce wood. The spent hydrolysates served as excellent growth media for the Cel7B-producing strain, A. niger D15[egI], which displayed higher endoglucanase activities in the spent hydrolysates than in standard medium with a comparable monosaccharide content (e.g., 2,100 nkat/ml in spent bagasse hydrolysate compared to 480 nkat/ml in standard glucose-based medium). In addition, A. niger D15[egI] was also able to consume or convert other lignocellulose-derived compounds, such as acetic acid, furan aldehydes, and phenolic compounds, which are recognized as inhibitors of yeast during ethanolic fermentation. The results indicate that enzymes can be produced from the stillage stream as a high-value coproduct in secondgeneration bioethanol plants in a way that also facilitates recirculation of process water. Copyright © 2009, American ...
The fungi Aspergillus niger and A. welwitschiae are morphologically indistinguishable species used for industrial fermentation and for food and beverage production. The fungi also occur widely on food crops. Concerns about their safety have arisen with the discovery that some isolates of both species produce fumonisin (FB) and ochratoxin A (OTA) mycotoxins. Here, we examined FB and OTA production as well as the presence of genes responsible for synthesis of the mycotoxins in a collection of 92 A. niger/A. welwitschiae isolates from multiple crop and geographic origins. The results indicate that (i) isolates of both species differed in ability to produce the mycotoxins; (ii) FB-nonproducing isolates of A. niger had an intact fumonisin biosynthetic gene (rum) cluster; (iii) FB-nonproducing isolates of A. welwitschiae exhibited multiple patterns of fum gene deletion; and (iv) OTA-nonproducing isolates of both species lacked the ochratoxin A biosynthetic gene (ota) cluster. Analysis of genome sequence data
Aspergillus niger ATCC ® 11414™ Designation: A-1-233 [72-4, A60, IMI 75353, NRRL 2270, VTT D-77050] Application: degrades apple pomace produces 6-phosphofructo-2-kinase produces ATP citrate lyase produces aconitic hydrase produces carnitine acetyltransferase produces citric acid citrate produces polygalacturonase endopolygalacturonase, pectinase transformation host produces citric acid from apple pomace
A rapid growing and high xylanase producing fungus Aspergillus niger SA7, was isolated from starch waste. The best yield of xylanase was 2400 U/g on the solid-state starch waste with 65-70% of initial moisture content at 28℃ for 3 days. Crude enzyme preparation contained more components of cellulase. Addition wheat bran to medium could stimulated xylanase production. 21.6% reducing sugar was obtained by hydrolysis starch waste at 45℃ culture for 24 hours.
TY - JOUR. T1 - Effect of carbon and nitrogen sources on the production of a highly enantioselective epoxide hydrolase from Aspergillus niger. AU - Morisseau, C.. AU - Venturi, G.. AU - Moussou, P.. AU - Baratti, J.. PY - 1998. Y1 - 1998. N2 - The highly enantioselective epoxide hydrolase from Aspergillus niger is well utilized as biocatalysts for the preparation of enantiopure chiral epoxides and diols. Both growth of the fungus and EH activity production were found greatly affected by changing the carbon or the nitrogen source with fructose and corn steep liquor being the best. Their concentrations were optimized (10 g.l-1 of fructose and 15 g.l-1 of corn steep) which resulted in an increase of both the biomass produced (31%) and the epoxide hydrolase specific activity (38%). The results obtained suggested a complex regulation of the EH production. On the whole, a two times increase of the total EH activity was obtained.. AB - The highly enantioselective epoxide hydrolase from Aspergillus ...
Microorganisms generally encounter a fluctuating environment in their natural habitat and similar conditions also happen in large-scale bioreactors. In this work, the dynamic response of intracellular and extracellular metabolites of Aspergillus niger was investigated after sudden exposure to high and low excess glucose concentrations in chemostats. It was found that the steady-state pathway turnover time of the carbon flux through the central carbon metabolism (CCM) was PP pathway 50 s, EMP pathway 20 s, and TCA cycle 189 s, and an upper limit for individual metabolite concentrations in the CCM was estimated. Regardless of the glucose pulse size, little changes of amino acids levels were observed except for aspartate, which showed a significant decrease. The ATP paradox, known from other organisms, was also observed in the studied A. niger strain. However, a different response of the NAD+/NADH ratio to the glucose pulses was found in A. niger compared to previously published observations on Penicillium
Nigerapyrones A-​H, α-​pyrone derivatives from the marine mangrove-​derived endophytic fungus Aspergillus niger MA-​132. Liu, Dong; Li, Xiao-Ming; Meng, Li; Li, Chun-Shun; Gao, Shu-Shan; Shang, Zhuo; Proksch, Peter; Huang, Cai-Guo; Wang, Bin-Gui. Journal of Natural Products (2011), 74(8), 1787-1791.. ...
Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient ...in raw ...
Dr. Chenyu Du has more than 15 years research experience in fermentation, metabolic engineering, bioprocess development and simulation, bio-separation and biomass pretreatment. He has published 30+ high-impact publications, including 30+ papers, 2 patents and 5 book chapters. Since joined the University of Nottingham, he generated research income over £570 k as PI (including fully funded PhD studentships), £14.3 m as Co-PI and supervised, co-supervised 10 PhD students, 2 MRes students, 15+ MSc students and 15+ undergraduates. At the University of Nottingham, Chenyu has been involved in the research pertaining to the Lignocellosic Conversion to Ethanol programme (LACE programme, funded by BBSRC Sustainable Bioenergy Centre). He developed a solid state fungal fermentation based pre-treatment strategy to convert lignocellulosic raw materials into a mixture of fermentable simple sugars. In this process, cellulolytic enzymes were produced by culturing fungus (Aspergillus niger) directly on the ...
0086]1. Aarestrup F. M. 1995. Occurrence of glycopeptide resistance among Enterococcus faecium isolates from conventional and ecological poultry farms. Microb Drug Resist. 1: 255-257. [0087]2. Abarca M. L, M. R. Bragulat, G. Castella and F. J. CabanesS. 1994. Ochratoxin A Production by Strains of Aspergillus niger var. niger. Applied and environmental Microbiology. 60: 2650-2652. [0088]3. Amann, R., Ludwig, W. & Schleifer, K. H. 1988. Subunit of ATP-synthase: a useful marker for studying the phylogenetic relationship of eubacteria. J Gen Microbiol. 134: 2815-2821. [0089]4. Ananthaswamy H. N and Eisenstark A (1977) Repair of hydrogen peroxide-induced single-strand breaks in Escherichia coli deoxyribonucleic acid. Journal of Bacteriology 130(1): 187-191. [0090]5. Angeles-Lopez M., E. G. Ramos E. G. C. and C. A. Santiago (2001) Hydrogen peroxide production and resistance to nonoxinol-9 in Lactobacillus spp. isolated from vagina of reproductive age women. Revista Latinoamericana de Microbiologia ...
Isopullulanase (IPU) from Aspergillus niger A.T.C.C. (American Type Culture Collection) 9642 hydrolyses pullulan to isopanose. IPU is important for the production of isopanose and is used in the structural analysis of oligosaccharides with α-1,4 and α-1,6 glucosidic linkages. We have isolated the ipuA gene encoding IPU from the filamentous fungi A. niger A.T.C.C. 9642. The ipuA gene encodes an open reading frame of 1695 bp (564 amino acids). IPU contained a signal sequence of 19 amino acids, and the molecular mass of the mature form was calculated to be 59 kDa. IPU has no amino-acid-sequence similarity with the other pullulan-hydrolysing enzymes, which are pullulanase, neopullulanase and glucoamylase. However, IPU showed a high amino-acid-sequence similarity with dextranases from Penicillium minioluteum (61%) and Arthrobacter sp. (56%). When the ipuA gene was expressed in Aspergillus oryzae, the expressed protein (recombinant IPU) had IPU activity and was immunologically reactive with ...
Xylan is a hemicellulose polysaccharide which is composed of β-1,4-linked d-xylosyl residues. Endo-1,4-β xylanase has the ability to cleave xylan back bone chains to release xylose residues. They are produced by a number of prokaryotic and eukaryotic organisms. Among them, filamentous fungi are attracting great attention due to high secretion of xylanolytic enzymes. Endo-1,4-β xylanase has wide industrial applications such as in animal feed, bread making, food and beverages, textile, bleaching of wood pulp, and biofuel production. In this study, different Aspergillus species were screened for the production of endo-1,4-β xylanase, and Aspergillus niger KIBGE-IB36 was selected for optimum production of enzyme in submerged fermentation technique. Influence of various fermentation conditions was investigated to produce high titer of endo-1,4-β xylanase. The results indicated that A. niger KIBGE-IB36 showed optimum production of endo-1,4-β xylanase at 30 °C, pH 8 after 6 days of incubation. Different
The catalytic domain of glucoamylases G1 and G2 from Aspergillus niger is produced in vitro in high yield by limited proteolysis using either subtilisin Novo or subtilisin Carlsberg. Purification by affinity chromatography on an acarbose-Sepharose column followed by ion-exchange chromatography on HiLoad Q-Sepharose leads to separation of a number of structurally closely related forms of domain. The cleavage occurs primarily between Val-470 and Ala-471 as indicated by C-terminal sequencing, whereas the N-terminus is intact. Subtilisin Carlsberg, in addition, produces a type of domain which is hydrolysed before Ser-444, an O-glycosylated residue. This leaves the fragment Ser-444-Val-470 disulphide-bonded to the large N-terminal part of the catalytic domain. Subtilisin Novo, in contrast, tends to yield a minor fraction of forms extending approx. 30-40 amino-acid residues beyond Val-470. The thermostability is essentially the same for the single-chain catalytic domain and the original glucoamylases ...
Abstract: The present investigation deals with the effect of vegetative inoculum on submerged citric acid fermentation by Aspergillus niger using stirred bioreactor. All fermentations were carried out following growth on 15% raw molasses sugars for 144 h. Ferrocyanide (200 ppm) was used to control the trace metals present in the molasses medium. The maximum production of citric acid was obtained when 1.0% vegetative inoculum was used. The dry cell mass and sugar consumption were 18.5 and 96.55 g l-1, respectively. The mycelia were intermediate round pellets in their morphology. The specific productivity (qp = 0.074±0.02a g g-1 cells h-1) was several folds higher than many other workers. ...
Aspergillus niger is a versatile filamentous fungus found in the environment all over the world in soil and on decaying plant material and it has been reported to grow on a large number of foods and feeds [1]. At the same time it is a popular production host for industrial fermentations and it is used for production of both organic acids and for indigenous and heterologous enzymes and proteins [2-4]. However, A. niger produces various secondary metabolites, and among those also the important mycotoxins fumonisin B2 (FB2) and ochratoxin A (OTA) [5, 6]. Due to the ubiquity of A. niger, its production of secondary metabolites is important both from a biotechnological and a food-safety viewpoint.. Secondary metabolites are small molecules that are not directly involved in metabolism and growth. Both plants and fungi are known for producing a large number of chemically diverse secondary metabolites. While the role of some of these metabolites makes sense biologically as inferring an advantage to the ...
BioAssay record AID 568480 submitted by ChEMBL: Antifungal activity against Aspergillus niger KCTC 1231 after 2 days by twofold broth dilution method.
BioAssay record AID 1085122 submitted by ChEMBL: Antifungal activity against Aspergillus niger at 100 ug/disk after 48 hr by disk diffusion test.
Spent refinery processing catalyst is listed as a hazardous waste; the toxicity characteristic leaching procedure (TCLP) extracts of the catalyst are found to contain heavy metals at concentrations exceeding the regulated levels. In the present investigation, Aspergillus niger was adapted to single metal ions Ni, Mo or Al (at 100-2000 mg/L in steps of 100 mg/L) and then to a mixture of Ni, Mo and Al (at a mass ratio of 1:2:6, as approximately present in the spent catalyst). Adaptation experiments with single metals showed that the fungus could tolerate up to 1000 mg/L Ni, 1200 mg/L Mo and 2000 mg/L Al. In the presence of a mixture of these metals, the fungus was able to tolerate up to 100 mg/L Ni, 200 mg/L Mo and 600 mg/L Al. One-step bioleaching experiments with 1 wt% spent catalyst (of particle size <37 μm) were carried out using un-adapted and various adapted fungal strains. In contrast to the adapted strains, the un-adapted strain showed no growth in the presence of the catalyst. ...
Continuous chemostat cultures of a recombinant strain of Aspergillus niger (B1-D), engineered to produce the marker protein hen egg white lysozyme, were investigated with regard to their susceptibility to oxidative stress. The culture response to oxidative stress, produced either by addition of exogenous hydrogen peroxide (H2O2) or by high dissolved oxygen tension (DOT), was characterised in terms of the activities of two key defensive enzymes: catalase (CAT) and superoxide dismutase (SOD). Since the morphology is so critical in submerged fungal bioprocesses, the key morphological indices were analysed using a semi-automated image analysis system. Both oxidant stressors, H2O2 and elevated DOT, increased both enzyme activities, however, the extent was different: exogenous H2O2 led mainly to increased CAT activity, whereas gassing with O2 enriched air, which resulted in a DOT of 165% of air saturation, increased both enzyme activities more than 2-fold compared with the control steady state ...
An acidophilic α-amylase from Aspergillus niger RBP7 was purified after solid state fermentation on potato peel substrate. Molecular mass of the purified α-amylase was 37.5 kDa and it exhibited 1.4 mg/ml and 0.992 μ/mol/min Km and Vmax values, respectively. The enzyme was stable in the pH range from 2.0 to 6.0, at high NaCl concentration (3 M) and at temperatures between 40 °C and 70 °C. The enzyme showed an optimal activity at pH 3.0 and at 45 °C. The enzyme was inhibited by Hg2+ and was stable in the presence of different surfactants (Tween 60, Tween 80, and SDS at 1% level) and different inhibitory reagents (β-mercaptoethanol, phenylmethylsulfonyl fluoride, and sodium azide). This acidophilic amylase enzyme can digest heterogeneous food materials, i.e. the mixture of rice, fish, bread and curry with comparable activity to the commercial diastase enzymes available.. ...
Purchase high purity enzyme endo-1-5-alpha-L-Arabinanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Naringinase from Aspergillus niger was prepared and characterized to evaluate its effectiveness in debittering citrus juice. The enzyme was purified to homogeneity by sulfate fractionation and chromatographies on Q-Sepharose, Sephacryl S-200, and S-1
A NOVEL STRAIN OF Aspergillus niger PRODUCING A COCKTAIL OF HYDROLYTIC DEPOLYMERISING ENZYMES FOR THE PRODUCTION OF SECOND GENERATION BIOFUELS
A total of 49 protein sequences of alkaline proteases retrieved from GenBank representing different species of Aspergillus have been characterized for various physiochemical properties, homology search, multiple sequence alignment, motif, and super family search and phylogenetic tree construction. The sequence level homology was obtained among different groups of alkaline protease enzymes, viz alk ...
We have started research to analyse the molecular mechanism underlying protein secretion in A. niger. In the first instance we have isolated 9 distinct GTPase encoding genes from A. niger (sarA, sagA-H) corresponding to GTPases involved in most stages of the secretory pathway. Interestingly, several of these genes homologues are present in higher eukaryotes, but not in S. cerevisiae. These genes are used to generate a set of (conditional/deletion) mutants imposing defined blocks in the secretory pathway. To analyse transport and secretion of proteins we have developed a GFP-based secretion reporter system by fusing GFP to a carrier protein, glucoamylase (GLA). Expression of a g1aA::gfp fusion construct resulted in fluorescence of the cell wall, probably representing secreted GLA::GFP fusion protein that is retained within the extracellular matrix. Periplasmic fluorescence was only observed in young mycelium. No periplasmic fluorescence is observed in older mycelia probably due to acidification ...
SBC) and E (7.5% untreated SBC). Total DMI rose (P , 0.05) with Aspergillus treated diets (B and D) than the untreated diets (C and E). The trend was similar for the rest of the variables (CPI and EEI) except for the crude fibre intake which did not show a specific trend. Dry matter digestibility for the control diet (A) was the lowest than any of the other diets while the highest dry matter digestibility was recorded ...
ID ASPNG_1_PE330 STANDARD; PRT; 155 AA. AC ASPNG_1_PE330; A2R3F4; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Putative uncharacterized protein An14g04100;Flags: DE Precursor; (ASPNG_1.PE330). GN ORFNames=An14g04100; OS ASPERGILLUS NIGER. OC Eukaryota; Fungi; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiales; OC Trichocomaceae; mitosporic Trichocomaceae; Aspergillus. OX NCBI_TaxID=5061; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ASPNG_1.PE330. CC Aspergillus niger chromosome I CADRE full sequence 1..3379275 annotated CC Ensembl Genomes CC -!- ANNOTATIONS ORIGIN:A2R3F4_ASPNC CC -!- GENE_FAMILY: HOG000175185 [ FAMILY / ALN / TREE ] DR HOGENOM:Aspergillus_niger;CADANGAG00011346;CADANGAT00011346;CADANGAP00011126. DR EMBL; AM270320; - ; DR UniProtKB/Swiss-Prot; A2R3F4; -. DR EMBL; AM270320; CAK46646.1; -; Genomic_DNA. DR RefSeq; XP_001401034.1; ...
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Endo-1,5-alpha-L-arabinanase involved in degradation of pectin. Its preferred substrate is linear 1,5-alpha-L-arabinan (By similarity).
The [email protected] Centre provides a platform for research students to deposit their Ph.D. theses and make it available to the entire scholarly community in open access. Shodhganga Mirror Site ...
Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is one o
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Common black mold found on plants. Produces abundant conidiophores. Medium: potato dextrose agar. Per plate.NOTE: Bacterial and fungal cultures will be sold and shipped to …
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An L-fucose lectin, ANL from the corneal smears of a mycotic keratitis patient was reported earlier. Interaction of ANL with immortalized Human Corneal Epithelial Cells (HCECs) was studied in order to assign the role of ANL in pathogenesis. ANL showed strong binding to HCECs which could be blocked by L-fucose and mucin. At concentrations below 0.6 μg/mL ANL showed proliferative effect and highest at 0.07 μg/mL leading to expression of proinflammatory cytokines IL-6 and IL-8. ANL induced proinflammatory response is mediated by TLR-2,-4, MyD88, NFkB and C-Jun dependent signaling. In contrast, ANL at concentrations above 0.6 μg/mL showed growth inhibitory effect at 48 h with an IC50 of 2.75 μg/mL. Western blot analysis revealed that HCECs treated with ANL at lower concentration induced the expression of proinflammatory signaling proteins TLR-2, 4, MyD88, NFkB and C-Jun which maintain high cell proliferating state. At higher concentration ANL induced apoptotic effect in HCECs with an increase in ...
Nai C, Meyer V. The beauty and the morbid: fungi as source of inspiration in contemporary art. Fungal Biology and Biotechnology 2016; 3:10 DOI: 10.1186/s40694-016-0028-4 Bignell E, Cairns T, Throckmorton K, Nierman W, Keller N. Secondary metabolite arsenal of an opportunistic pathogenic fungus. Phil. Trans. R. Soc. B; DOI: 10.1098/rstb.2016.0023 Paege N, Jung S, Schäpe P, Müller-Hagen D, Ouedraogo JP, Heiderich C, Jedamzick J, Nitsche BM, van den Hondel CA, Ram AF, Meyer V. A Transcriptome Meta-Analysis Proposes Novel Biological Roles for the Antifungal Protein AnAFP in Aspergillus niger. PlosOne 2016; DOI:10.1371/journal.pone.0165755.s009 Meyer V, Idnurm A. The freedom of choice. Fungal Biology and Biotechnology 2016; 3:9 DOI: 10.1186/s40694-016-0027-5 Meyer V, Andersen M, Brakhage A, Braus G, Caddick M, Cairns T, de Vries R, Haarmann T, Hansen K, Hertz-Fowler C, Krappmann S, Mortensen U, Peñalva M, Ram AF, Head R. Current challenges of research on filamentous fungi in relation to human ...
2.1.1 Absorption, distribution, and excretion No available data. 2.2 Toxicological studies 2.2.1 Acute studies Species Route LD50 Reference (mg/kg/b.w.) Rat oral 5000 van Eeken et al., 1986a 2.2.2 Short-term studies 2.2.2.1 Rat Groups of 9-20 male and 10-20 female Wistar rats, 89-111 g b.w., were treated by gavage for 91 days with chymosin at daily dose levels of 0, 50, 5000 or 1000 mg/kg b.w. The report did not indicate whether the commercial enzyme preparation or a more concentrated or purified form of the enzyme was used in the study. No mortality was observed in experimental animals over the course of the study. Growth, behaviour and external appearance were normal. Haematology measurements in treated animals were comparable to controls. Statistically significant changes were occasionally observed in clinical chemistry parameters, but the changes were not dose-related. Absolute and relative organ weights were unaffected by treatment, except for liver weights in females, which exhibited a ...
Abstract: The effect of solid state fermentation of Aspergillus niger on millets nutritional quality and phytic acid content were investigated in this study. Ammonium sulphate and urea as Nitrogen sources were used in combination with millet at 10 gN kg 1 substrate. The mixture was fermented for 84 h at 35 C and 90-95% RH in the laboratory. The results indicated that the crude protein content of millet was raised from 10.96-17.33%, while the crude lipid was lowered fro 3.12-2.58%. The ash content value was greatly increased from 1.36-7.94%. Five percent changes in minerals (calcium, magnesium and phosphorus) content values were also observed. Phytic acid content value of 0.4 mg 100g 1 reduced significantly to minimal 0.015 mg 100g 1. ...
An existing way to produce glucose uses enzymes to hydrolyzepolysaccharides. Aspergillusniger is recognized to produce glucoamylases capable to reduce starch to...
A team of researchers is working to use naturally-occurring fungi for an environment-friendly recycling process to extract cobalt and lithium from waste batteries.. Get the Free Tracker App to find a SNES Classic in Stock. The idea first came from a student who had experience extracting some metals from waste slag left over from smelting operations, said Jeffrey A. Cunningham, Ph.D. and the projects team leader.. Cunninghams team is developing the environmentally-safe way to do this with organisms found in nature -- fungi in this case -- and putting them in an environment where they can do their work.. Fungi are a very cheap source of labor, he pointed out in his work to be presented at the 252nd National Meeting & Exposition of the American Chemical Society (ACS).. To drive the process, Cunningham and Valerie Harwood, Ph.D., both at the University of South Florida, are using three strains of fungi -- Aspergillus niger, Penicillium simplicissimum and Penicillium chrysogenum.. The team ...
Aspergillus niger endopolygalacturonase E: a cell degrading enzyme found in microorganisms and higher plants; nucleotide sequence in first source; GenBank Y14386
Significant dropping of infectious disease is owned greatly to antibiotic development industry. Due to prevalence of drug resistance organisms alongside with other problems, scientists are focusing on medicinal plants. Medicinal plants and their antimicrobial components are attractive alternatives for antibiotics. In the present project, the antimicrobial effects of methanolic extract and fractions of aerial parts of Euphorbia splendida Mobayen were evaluated against Aspergillus niger, Aspergillus fumigatus, Candida albicans, and Candida krusei. Euphorbiasplendida samples were transferred to laboratory after confirmation. The plant samples were then shadow dried before being powder. Different extracts and fractions were prepared by standard methods. The extracts and fractions were kept under standard conditions until used. The antifungal assessment was performed by standard agar diffusion methods. The concentration of 1000 mg/ml of each of methanolic, hexanic, chloroformic and aqueous extract
A gene of glucose oxidase (GOD) from Aspergillus niger Z-25 was cloned and sequenced. The entire open reading frame (ORF) consisted of 1,818 bp and encoded a putative peptide of 605 amino acids. The gene was fused to the pPICZαA plasmid and overexpressed in Pichia pastoris SMD1168. The recombinant GOD (rGOD) was secreted into the culture using MF-α factor signal peptide under the control of the AOX1 promoter. Sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated that rGOD exhibited a single band at around 94 kDa. The maximal GOD activity of approximately 40 U/mL was achieved in shake flask by induction under optimal conditions after 7 days. rGOD was purified by ammonium sulfate precipitate leading to a final specific activity of 153.46 U/mg. The optimum temperature and pH of the purified enzyme were 40 °C and 6.0, respectively. Over 88% of maximum activity was maintained below 40 °C. And the recombinant enzyme displayed a favorable stability in the pH range from 4.0 to 8.0. The ...
4665-7604 recognises glucose oxidase from Aspergillus niger.This antibody has not been cross-adsorbed and may react with homologous proteins
glucose oxidase from aspergillus niger/ACM9001370 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
The secondary substrate binding site (SBS) of Bacillus subtilis and Aspergillus niger glycoside hydrolase family 11 xylanases was studied by site-directed mutagenesis and evaluation of activity and binding properties of mutant enzymes on different substrates. Modification of the SBS resulted in an up to three-fold decrease in the relative activity of the enzymes on polymeric versus oligomeric substrates and highlighted the importance of several amino acids in the SBS forming hydrogen bonds or hydrophobic stacking interactions with substrates. Weakening of the SBS increased Kd values by up to 70-fold in binding affinity tests using natural substrates. The impact that modifications in the SBS have both on activity and on binding affinity towards polymeric substrates clearly shows that such structural elements can increase the efficiency of these single domain enzymes on their natural substrates ...
Kinetics of Escherichia coli destruction by microwave irradiation. Tailing of thermal inactivation curve of Aspergillus niger spores
Good performance of p16/ki-67 dual-stained cytology for surveillance of women treated for high-grade CIN. Possible explanation of the basic human capacity for emotional experience Dephosphorylation of phytate by levitra vs viagra using the Aspergillus niger phytase with a high affinity for phytate. Identification of viagra substitute DNA-reactive B cells in patients with systemic lupus erythematosus. Contribution of exogenous genetic elements to the group A Streptococcus metagenome.. The FA-CS nanoparticles (NPs) were prepared by crosslinking FA-CS conjugates with sodium tripolyphosphate (STPP). The differentiation level could be sildenafil citrate estimated by two-dimensional cell morphology analysis using a conventional phase-contrast microscope. The observed preferences cannot be explained by any single structural principle, but point instead to the interaction of several principles. Catechols are co-planar cis-diols known to form female viagra pills stable, isolable complexes with borate ...
TY - JOUR. T1 - RNA-sequencing reveals the complexities of the transcriptional response to lignocellulosic biofuel substrates in Aspergillus niger. AU - Pullan, Steven T. AU - Daly, Paul. AU - Delmas, Stéphane. AU - Ibbett, Roger. AU - Kokolski, Matthew. AU - Neiteler, Almar. AU - van Munster, Jolanda M. AU - Wilson, Raymond. AU - Blythe, Martin J. AU - Gaddipati, Sanyasi. AU - Tucker, Gregory A. AU - Archer, David B. PY - 2014/11/17. Y1 - 2014/11/17. N2 - BACKGROUND: Saprobic fungi are the predominant industrial sources of Carbohydrate Active enZymes (CAZymes) used for the saccharification of lignocellulose during the production of second generation biofuels. The production of more effective enzyme cocktails is a key objective for efficient biofuel production. To achieve this objective, it is crucial to understand the response of fungi to lignocellulose substrates. Our previous study used RNA-seq to identify the genes induced in Aspergillus niger in response to wheat straw, a biofuel ...
Hypersensitivity pneumonitis (HP), also referred to as extrinsic allergic alveolitis (EAA), is an inflammatory lung disease resulting from the inhalation and subsequent sensitization to a wide variety of inhaled organic dusts.1-5 HP is not mediated by IgE. It is associated with progressive pulmonary disability, irreversible lung damage, and mortality in some occupational settings.1-5 Patients often present with intermittent chills, fever, cough, and shortness of breath that begin four to eight hours after exposure to the offending dust. Aspergillus mold can be found in soil, foods, fresh water, and other natural sources including compost, barley, tobacco, and Esparto grass dust (stucco).1,3,5 These organisms grow well in decaying organic material at temperatures often attained during decomposition.5Aspergillus mold can also grow on ceiling and walls where water damage has occurred.6. No single laboratory test is diagnostic for hypersensitivity pneumonitis.1-5 Diagnosis is based on a complete ...
报告1:Moving from descriptive to predictive biology: Rational rewiring of the cell factory Aspergillus niger 报 告 人:Vera Meyer 德国柏林工业大学教授 报告题目:Moving from descriptive to predictive biology: Rational rewiring of the cell factory Aspergillus niger 报告时间:5月8日 9:30-10:30 报告地点:天津工业生物所C408会议室 报告摘要:Fungal biotechnology is currently undergoing a renaissance with important implications for its role as platform technology for the sustainable production of products, goods and drugs. Allied to this are the recent advances in fungal Systems and Synthetic Biology which can be seen as two complementary approaches to investigate the complexity of biological systems including fungi. Whereas Systems Biology analyzes cellular systems in an iterative cycle of high-throughput generation of omics data and modeling, Synthetic Biology takes a constructive approach to reengineer biological networks and to design novel ...
Books and Book Chapters. 1. Lactase production by Aspergillis Oryzae , Lambert publications, Germany, 2012. 2. Cellulase production by Aspergillus niger , Lambert publications , Germany, 2012. 3. Biological synthesis, Characterization and antimicrobial activity of silver nanoparticles from beetle leaves, In Nanoscience and drugdelivery. Apple science publication USA. Projects:. 1. Major Research project on Cellulase production by mutant fungal strain Aspergillus niger Sanctioned by University Grants Commision 2009. New Delhi, India. Abstracts. Indian Science Abstracts:. ...
Aspergillus tubingensis XghA protein: pectinolytic enzyme from Aspergillus tubingensis; amino acid sequence in first source; GenBank AJ249460
β-glucosidase production was maximum on untreated kallar grass (Leptochloa fusca) compared to other substrates (wheat bran and sigma cell-20 cellulose). But in the presence this substrate maximum amount of polysaccharides were also produced. Three types of polysaccharides (high, low and intermediate molecular weight), non covalently attached to glucosidase, were observed when Aspergillus niger NIAB 280 was grown on kallar gray wheat bran and salicin in combination. High and intermediate molecular weight polysaccharioc were alpha linked to B-glucosidase. The low molecular weight polysaccharide streak was to kallar grass and could be hemicellulose-lignin complex. Polysaccharides non-covalent attached to B-glucosidase were removed by specially designed compartmental electrophore apparatus. The enzyme was partially purified and concentrated. The non-covalently attached polysaccharides were directly associated with the B-glucosidase stability. The polysaccharide glucosidase complex was extremely ...
1CF3: 1.8 and 1.9 A resolution structures of the Penicillium amagasakiense and Aspergillus niger glucose oxidases as a basis for modelling substrate complexes.
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This enzyme blend provides a broad acting formulation derived from the plant sources Aspergillus oryzae and Aspergillus niger. It is a vegetarian alternative to Pancreatic Enzyme Formula for carbohydrate, lipid and protein digestion. These enzymes have several unique properties that enhance their ability to promote the optimal breakdown of foods and nutrients, including broad substrate specificity. This means that these enzymes have the ability to recognize and help digest a large number of target molecules. Plant enzymes are also active in a wide pH range, from about 3.5-8.5, indicating that their actions begin in the stomach and continue in the small intestine, promoting complete digestion. Pure Encapsulations Pancreatic VegEnzymes is comparable in amylase, protease and lipase profile to Pancreatic Enzyme Formula ...
Endoglucanase B (EGLB) derived from Aspergillus niger BCRC31494 has been used in the food fermentation industry because of its thermal and alkaline tolerance. It was cloned and expressed in Pichia pastoris. According to sequence analysis, the gene open reading frame comprises 1,217 bp with five introns (GenBank GQ292753). According to sequence and protein domain analyses, EGLB was assigned to glycosyl hydrolase family 5 of the cellulase superfamily. Several binding sites were found in the promoter region. The purified recombinant enzyme was induced by 0.5% methanol, and it exhibited optimal activity at 70 °C and pH 4. EGLB was stable for 3 h at temperatures below 60 °C, with more than 90% of its activity remaining. The enzyme was specific for substrates with β-1,3 and β-1,4 linkages. In Lineweaver-Burk plot analysis, the Km and Vmax values of EGLB for β-D-glucan were 134 mg/mL and 4.68 U/min/mg, respectively. The enzyme activity was increased by 1.86-fold by Co2+ and by 2-fold by Triton X-100 and
1.Ratananikom, K., Choengpanya, K., Tongtubtim, N., Charoenrat, T., Withers, S.G. and Kongsaeree, P.T. (2013) Mutational analysis in the glycone binding pocket of Dalbergia cochinchinensis -glucosidase to increase catalytic efficiency towards mannosides. Carbohydr. Res. 373, 35-41.. 2.Thongpoo, P., McKee, L.S., Araújo, A.C., Kongsaeree, P.T. and Brumer, H. (2013) Identification of the acid/base catalyst of a glycoside hydrolase family 3 (GH3) -glucosidase from Aspergillus niger ASKU28. BBA - Gen Subjects 1830, 2739-2749.. 3.Stepper, J., Dabin, J., Eklof, J.M., Thongpoo, P., Kongsaeree, P., Taylor, E.J., Turkenburg, J.P., Brumer, H. and Davies, G.J. (2013) Structure and activity of the Streptococcus pyogenes family GH1 6-phospho--glucosidase SPy1599. Acta Cryst. D69, 16-23.. 4.Sukkhum, S., Tokuyama, S., Kongsaeree, P., Tamura, T., Ishida, Y. and Kitpreechavanich, V. (2011) A novel poly (L-lactide) degrading thermophilic actinomycetes, Actinomadura keratinilytica strain T16-1 and pla ...
Three approaches for enrichment of Penicillium verruculosum cellulose multienzyme cocktails with a heterologously expressed Aspergillus niger beta-glucosidase (AnBGL) for boosting the hydrolytic performance of cellulases were tested. The best boosting effects (73-113%) on glucose yield in 72-h hydrolysis of milled aspen wood and Avicel provided the enzyme preparation obtained by cofermentation of two P. verruculosum strains, one of which was a basic high-cellulase strain, while the other was capable to produce the heterologously expressed AnBGL (up to 80% of the total protein when used in a separate fermentation). Two other approaches, based on individual fermentations of P. verruculosum strains, carrying the heterologous AnBGL under the control of either a strong cbh1 gene promoter or a weaker hist4 gene promoter, also provided notable boosting effects, although they were less pronounced than in the first mentioned case. (C) 2015 Elsevier Ltd. All rights reserved ...
This study investigates the use of palladised Escherichia coli as a biometallic catalyst with a view to its use in deracemisation reactions. Some bacteria can reduce metals by their use as the terminal electron acceptor in the respiratory chain, and recent work using Desulfovibrio spp. has led to the generation of a palladium bionanocatalyst with superior activity to commercially available carbon-supported Pd(0) nanoparticles. This current study has investigated the use of formate as an electron donor for the reduction of soluble Pd(II) to insoluble Pd(0) nanoparticles by E. coli, and investigates the biological mechanisms responsible for the bioreduction, and for the extracellular location of the nanoparticles.Previous work has been done at the Manchester Interdisciplinary Biocentre with the directed evolution of the monoamine oxidase enzyme of Aspergillus niger (known as MAO-N), which catalyses the oxidative deamination of terminal amines. The result has been to produce an enzyme with enhanced ...
Aim: To synthesize a series of novel compounds namely, 4-(3-((substituted)-1H-pyrazol-3-yl-methylsulphonyl)-5-((3,5,6-trichloro-pyridin-2-yl-oxymethyl)-[1,2,4]triazol-4-yl-methyl)-morpholine (8a-g) and evaluate their antimicrobial activity. Material and Method: The chemical structures of newly synthesized compounds were elucidated by IR, 1H NMR, mass spectral and elemental analysis data. Their antimicrobial activities against Staphylococus aureus NCCS 2079, Bacillus Cereus, NCCS 2106, Escherichia coli NCCS 2065, Pseudomanas aeruginos NCCS 2200, Aspergillus niger ...
Commission Implementing Regulation (EU) 2017/1896 of 17 October 2017 concerning the authorisation of a preparation o endo-1,3(4)-beta-glucanase (EC 3.2.1.6) and endo-1,4-beta-xylanase (EC 3.2.1.8) produced by Aspergillus niger (NRRL 25541) as a feed additive for chickens for fattening, laying hens, pigs for fattening, minor poultry species and minor porcine species for fattening and amending Regulation (EC) No 255/2005 and repealing Regulation (EC) No 668/2003 (holder of the authorisation Andrés Pintaluba S.A ...
Literature References: Prepd by oxidation of glucose: H. Hlasiwetz, J. Habermann, Ann. 155, 120 (1870); J. Habermann, ibid. 162, 297 (1872). Fermentative prepn using Aspergillus niger: K. Bernhauer, L. Schulof, US 1849053 (1932 to Pfizer); A. J. Moyer et al., Ind. Eng. Chem. 32, 1379 (1940). Review of prepns and uses: F. J. Prescott et al., ibid. 45, 338 (1953); M. Roehr et al. in Biotechnology, Vol. 6, H. Rehm, G. Reed, Eds. (VCH, Weinheim, 2nd ed, 1996) pp 347-362. See also Gluconolactone. ...
Biotransformation of 3-hydroxydibenzo-α-pyrone into 3,8 dihydroxydibenzo-α-pyrone and aminoacyl conjugates by Aspergillus niger isolated from nativeshilajit ...
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Aspergillus niger • Colletotrichum musae • Colletotrichum gloeosporioide • Botryodiplodia theobromae • Fusarium monoliforme • ...
... and Aspergillus oryzae, but less effective on Fusarium sp. and Aspergillus niger. The UMY15 strain also proved very effective ...
Bahl OP (1970). "Glycosidases of aspergillus niger. II. Purification and general properties of 1,2-alpha-L-fucosidase". J. Biol ...
"Studies of Aspergillus niger. Part II. Transglycosidation by Aspergillus niger". J. Chem. Lond. Soc.: 3588-3593. doi:10.1039/ ...
Seeds collected from the wild often are heavily contaminated with spores of the fungus Aspergillus niger var. phoenicis, which ... Cooper-Driver, G.A., C. Wagner and H. Kolberg (2000). Patterns of Aspergillus niger var. phoenicis (Corda) Al-Musallam ... Infection of the cones and seeds of Welwitschia mirabilis by Aspergillus niger var. phoenicis in the Namib-Naukluft Park. South ... The fungicide tebuconazole may be useful in controlling limited A. niger seed infection. The population of Welwitschia ...
NEILSON NE (1955). "The aconitase of Aspergillus niger". Biochim. Biophys. Acta. 17 (1): 139-40. doi:10.1016/0006-3002(55)90330 ...
Ramakrishnan, C.V.; Martin, S.M. (1955). "Isocitric dehydrogenase in Aspergillus niger". Arch. Biochem. Biophys. 55: 403-407. ...
Black mold caused by Aspergillus niger; Leaf spot, and others, caused by Alternaria alternata; Bitter rot caused by Glomerella ...
Die fadenpilze, aspergillus flavus, niger u. fumigatus; eurotium repens (u. aspergillus glaucus) und ihre beziehungen zur ... otomycosis aspergillina, 1883 - The filamentous fungi, Aspergillus flavus, niger and fumigatus; Eurotium repens (Aspergillus ...
Aspergillus spp., Fusarium spp., Penicillium chrysogenum, Aspergillus niger and Candida albicans. An increased die-off of ... Aspergillus niger growth occurred on the aluminium coupons; growth was inhibited on and around copper coupons. Antimicrobial ... Aspergillus niger, Bacterium linens, Bacillus megaterium, Bacillus subtilis, Brevibacterium erythrogenes, Candida utilis, ...
Properties of amine oxidase of Aspergillus niger". Agric. Biol. Chem. 29: 864-869. doi:10.1271/bbb1961.29.864. Yamada H, Adachi ... Further properties of amine oxidase of Aspergillus niger". Agric. Biol. Chem. 29: 912-917. doi:10.1271/bbb1961.29.912. Boyer, P ... Purification and crystallisation of amine oxidase of Aspergillus niger". Agric. Biol. Chem. 29: 649-654. doi:10.1271/bbb1961.29 ...
Bardalaye, P.C., and Nordin, J.H. (1976). Galactosaminogalactan from cell walls of Aspergillus niger. J Bacteriol 125, 655-669 ... In Aspergillus fumigatus, a causative agent of aspergillosis, galactosaminogalactan is required for adherence to host tissue, ... 2013). Aspergillus Galactosaminogalactan Mediates Adherence to Host Constituents and Conceals Hyphal β-Glucan from the Immune ... Overlapping and distinct roles of Aspergillus fumigatus UDP-glucose 4-epimerases in galactose metabolism and the synthesis of ...
This enzyme can be found in Aspergillus niger. The enzyme 4-methoxybenzoate monooxygenase (O-demethylating) transforms 4- ...
Premkumar R, Rao PV, Sreeleela NS, Vaidyanathan CS (1969). "m-Hydroxybenzoic acid 4-hydroxylase from Aspergillus niger". Can. J ...
Rao PV, Moore K, Towers GH (1967). "O-pyrocatechiuc acid carboxy-lyase from Aspergillus niger". Arch. Biochem. Biophys. 122 (2 ...
Aspergillus niger, has no known sexual cycle. Thus Aspergillus niger is considered a form taxon. In contrast, isolates of its ... are used to improve both the taste and the texture of cheeses Aspergillus oryzae Aspergillus sojae Aspergillus niger ... Hence the formerly classified Aspergillus species is now properly called Emericella nidulans. Phylogenetic classification of ... close relative, Aspergillus nidulans, revealed it to be the anomorphic stage of a teleomorph (the ascocarp or fruiting body of ...
Orobol, an isoflavone, can be isolated from Aspergillus niger. in yeasts Aromatic alcohols (example: tyrosol) are produced by ...
Aspergillus niger acid proteinase A, Aspergillus niger var. macrosporus aspartic proteinase) is a proteolytic enzyme. The ... Iio, K.; Yamasaki, M. (1976). "Specificity of acid proteinase A from Aspergillus niger var. macrosporus towards B-chain of ... Effects of acid protease-specific inhibitors on the acid proteases from Aspergillus niger var. macrosporus". J. Biochem. 80: ... Tyr26-Thr This enzyme is isolated from Aspergillus niger var. macrosporus. Chang, W.J.; Horiuchi, S.; Takahashi, K.; Yamasaki, ...
Aspergillus niger and Candida albicans fungal spores. Hence, the potential to help prevent the spread of fungi that cause human ... Copper and copper alloy surfaces have demonstrated a die-off of Aspergillus spp., Fusarium spp., Penicillium chrysogenum, ...
Ninnekar, H; Vaidyanathan, C (1981). "Catechol 1,2-dioxygenase from Aspergillus niger: Purification and properties". J. Indian ... Aspergillus niger, Brevibacterium fuscum, Acinetobacter calcoaceticus, Trichosporon cutaneum, Rhodococcus erythropolis, ...
"Purification and characterization of intracellular alpha-glucuronidase from Aspergillus niger". Biosci. Biotechnol. Biochem. 56 ...
It was developed to grow Aspergillus niger and Penicillium camemberti. It works well for many saprophytic fungi and soil ... Dox, Arthur Wayland (1910). "The intracellular enzyms of penicillium and aspergillus: with special reference to those of ... bacteria such as species of Aspergillus, Candida, Penicillium, and Paecilomyces. Friedrich Czapek's original recipe is as ...
and Aspergillus niger, are efficient at removing these toxic compounds. EPSs contain enzymes such as oxidoreductase and ... and Zooglea' spp.) galactosaminogalactan (Aspergillus spp.) gellan (Aureomonas elodea and Sphingomonas paucimobilis) glucuronan ...
Hanif, A (2004). "Induction, production, repression, and de-repression of exoglucanase synthesis in Aspergillus niger". ... "Biocatalytic potential of laccase-like multicopper oxidases from Aspergillus niger". Microbial Cell Factories. 11 (1): 165. doi ... Laccase activity has been documented in T. reesei, in some species in the Aspergillus genus and in freshwater ascomycetes. ...
Fungi are also found, including Aspergillus niger and Candida krusei. Although these pathogens and fungi utilize the wasp's ... Aspergillus niger, which creates aflatoxins that can lead to respiratory disease and candidiasis; and Candida krusei, which can ...
They can be extracted from fungi such as Aspergillus niger. The fungus produces these enzymes to break down the middle lamella ...
Sakano, Y.; Masuda, N.; Kobayashi, T. (1971). "Hydrolysis of pullulan by a novel enzyme from Aspergillus niger". Agric. Biol. ...
It can be isolated from Aspergillus niger or Streptomyces neyagawaensis. It is a potent inhibitor of Phosphoinositide 3-kinase ...
"Microbial transformations of flavanone by Aspergillus niger and Penicillium chermesinum cultures". Journal of Molecular ...
He eventually found that these bacteria could only thrive adjacent to Aspergillus niger if glucose was not present. He ... In 1928, he was experimenting with the common fungus, Aspergillus niger. Müller noted that this fungus prevented some bacteria ...
... organism found and responsible for almost all pu'er fermentation has been identified in numerous studies as Aspergillus niger, ... "Aspergillus luchuensis, an industrially important black Aspergillus in East Asia". PLoS ONE. 8 (5): e63769. doi:10.1371/ ... Isolation and identification of Aspergillus species from the post fermentative process of Pu-Er ripe tea]. Acta Botanica ... Control over the multiple variables in the ripening process, particularly humidity and the growth of Aspergillus spp., is key ...
... optimization and production of cellulase by Aspergillus niger from agricultural waste". Journal of Pure and Applied ...
Aspergillus niger fungus liquor awamori [2] Aspergillus fumigatus fungus chocolate [1] Aspergillus oryzae fungus soy miso [2][5 ...
Aspergillus carbonarius, Aspergillus glaucus, Aspergillus melleus, and Aspergillus niger.[25][26][27] Because Aspergillus niger ... Ochratoxin A production by strains of Aspergillus niger var. niger. Appl. Environ. Microbiol. 60:2650-2652. ... Ochratoxin A production by Aspergillus carbonarius and A. niger isolates and detection using coconut cream agar. J. Food Mycol ... Members of the ochratoxin family have been found as metabolites of many different species of Aspergillus, including Aspergillus ...
Hariliku sibula eeterlikud õlid toimivad Aspergillus niger, Cladosporium werneckii, Candida albicans, Fusarium oxysporium, ...
FOS can also be synthesized by enzymes of the fungus Aspergillus niger acting on sucrose. GOS is naturally found in soybeans ...
... and citric acid produced by the mould fungus Aspergillus niger.[95] Microorganisms are used to prepare bioactive molecules such ...
C and pH 10 while a catalase derived from Aspergillus niger had a half life of 15 seconds under the same conditions. The ...
Aspergillus niger, Serpula himantioides, and Trametes versicolor.[18] These fungi have been found to corrode apatite and galena ... The order of most to least oxalic acid secreted by the fungi studied are Aspergillus niger, followed by Serpula himantioides, ... certain fungi such as Aspergillus niger and Paecilomyces javanicus can tolerate it. ...
Cooper-Driver, G.A., C. Wagner and H. Kolberg (2000). Patterns of Aspergillus niger var. phoenicis (Corda) Al-Musallam ... Semena, zbrana iz narave, so pogosto močno onesnažena s sporami glive Aspergillus niger var. phoenicis, [28] ki hitro ...
Beano contains the enzyme α-GAL, which is derived from the fungus Aspergillus niger. The enzyme works in the digestive tract to ...
The fungi Aspergillus flavus,[108] Aspergillus fumigatus,[108] Aspergillus niger,[108] Aspergillus parasiticus, Aspergillus ... tamarii, Aspergillus sulphureus, Aspergillus repens, Mucor hiemalis (not a human pathogen), Penicillium chrysogenum, ... Oven treatment killed conidia of A. fumigatus, A. flavus and A. niger, and did not degrade the active component of marijuana, ... Penicillium italicum and Rhizopus nigrans have been found in moldy cannabis.[107] Aspergillus mold species can infect the lungs ...
Aspergillus glaucus. Aspergillus lentulus. Aspergillus nidulans. Aspergillus niger. Aspergillus ochraceus. Aspergillus oryzae. ... Aspergillus restrictus. Aspergillus sojae. Aspergillus sydowii. Aspergillus tamari. Aspergillus terreus. Aspergillus ustus. ... Aspergillus alliaceus. Aspergillus caesiellus. Aspergillus caespitosus. Aspergillus candidus. Aspergillus carneus. Aspergillus ... Aspergillus deflectus. Aspergillus egyptiacus. Aspergillus fischerianus. Aspergillus flavus. Aspergillus foetidus. Aspergillus ...
Aspergillus niger, and Aspergillus sydowii can also effectively degrade PVC.[60] Phanerochaete chrysosporium was grown on PVC ... The fungus Aspergillus fumigatus effectively degrades plasticized PVC.[58] Phanerochaete chrysosporium has been grown on PVC in ...
In 1917, American food chemist James Currie discovered certain strains of the mold Aspergillus niger could be efficient citric ... Lotfy, Walid A.; Ghanem, Khaled M.; El-Helow, Ehab R. (2007). "Citric acid production by a novel Aspergillus niger isolate: II ... In this production technique, which is still the major industrial route to citric acid used today, cultures of A. niger are fed ...
and Aspergillus niger, are efficient at removing these toxic compounds.[15] EPSs contain enzymes such as oxidoreductase and ...
... eta Aspergillus niger[75] dira. Fungikoak ez diren mikroorganismoen artean Bacillus subtilis dago.[80] ... Ogia erasotzen duten onddo urdin espezieak honakoak dira: Aspergillus[75] eta Penicillium[76], baita Monilia sitophila espeziak ...
Orobol, an isoflavone, can be isolated from Aspergillus niger. in yeasts. Aromatic alcohols (example: tyrosol) are produced by ...
Aspergillus flavus, and Aspergillus niger. It causes mummification of the brood of a honey bee colony. The fungi are common ... Stonebrood (aspergillosis larvae apium) is a fungal disease caused by Aspergillus fumigatus, ...
Aspergillus rot Aspergillus niger Black rot of grapes Guignardia bidwellii Botrytis (Grey Rot or Noble Rot) Botrytis cinerea ...
"A two-step bioconversion process for vanillin production from ferulic acid combining Aspergillus niger and Pycnoporus ...
Fusarium oxysporium, F. moniliformis, Aspergillus Niger and A. paraciticus Local anaesthetic. S. acmella Murr.. NA. Aqueous. ... Candida species and Aspergillus species Antimicrobial. S. acmella Linn.. Flower heads. Petroleum ether. ...
In diabetic patients it may be caused by Aspergillus niger. It is very rarely caused by Aspergillus flavus, Aspergillus oryzae ... Serum precipitins for aspergillus (blood test to detect antibodies to aspergillus) Almost all aspergillomas are caused by ... Aspergillus terreus or Aspergillus nidulans. Patients with single aspergillomas generally do well with surgery to remove the ... is a long-term aspergillus infection of the lung and Aspergillus fumigatus is almost always the species responsible for this ...
Aspergillus niger. *Assisted reproductive technology. *Axenic. B. *Bacterial conjugation. *Bacterial therapy. *BamHI ...
Eel-vitamiin D2 moodustab Aspergillus niger steroididest ca 4/5, ning kogu ...
Aspergillus niger Strain:ATCC 1015 (DOE Joint Genome institute). *Aspergillus niger Strain:CBS 513.88, industrial use (2007[21] ... Aspergillus oryzae Strain:RIB40, industrial use (2005[22]). *Aspergillus terreus NIH 2624, statin producer and pathogen (2005, ... "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88". Nat. Biotechnol. 25 (2): 221-31. ... "Home - Aspergillus carbonarius ITEM 5010 v3". genome.jgi-psf.org. Retrieved 24 November 2018.. ...
... can be synthesized from tryptophan in the lab using Aspergillus niger and Psilocybe coprophila as catalysts. The ... Asperigillus niger would be the catalyst for this first phase. The second phase to synthesizing tryptophan itself from the 5- ...
Majs genetisk modificeret med genet for enzymet phytase fra skimmelsvampen Aspergillus niger.[18] ...
Aspergillus niger ATCC ® 64045™ Designation: OSU A-1 Application: assay of preservatives in wood fungus resistance testing ... Aspergillus niger van Tieghem (ATCC® 64045™) Strain Designations: OSU A-1 / Product Format: freeze-dried ... Standard Test Method for Detection and Estimation of Retention of Wood Preservatives by Aspergillus Bioassaying. West ... The influence of method variables on the Aspergillus bioassay of wood preservatives. Holzforschung 40: 131-136, 1986. ...
Aspergillus niger ATCC ® 6275™ Designation: 4247 [AM 324, CBS 131.52, CBS 769.97, DSM 1957, IFO 6341, IMI 45551, J. Friedrich ... Aspergillus niger van Tieghem (ATCC® 6275™) Strain Designations: 4247 [AM 324, CBS 131.52, CBS 769.97, DSM 1957, IFO 6341, IMI ... The use of Aspergillus niger for bioconversion of apple distillery waste. Eur. J. Appl. Microbiol. Biotechnol. 17: 243-247, ... Interesterification of butter fat by partially purified extracellular lipases from Pseudomonas putida, Aspergillus niger and ...
... was expressed in the protease deficient filamentous fungus Aspergillus niger AB1.13. Fungal transformants were obtained in ... Expression of catalytic subunit of bovine enterokinase in the filamentous fungus Aspergillus niger J Biotechnol. 2000 Jan 21;76 ... was expressed in the protease deficient filamentous fungus Aspergillus niger AB1.13. Fungal transformants were obtained in ... The secreted EK(L) was easily purified from other proteins found in A. niger culture supernatant, using ion exchange and ...
... by using the soil fungus Aspergillus niger 2587. The results recorded from UV-vis spectrophotometer and transmission electron ... Here, we have synthesized the silver nanoparticles (AgNPs) by using the soil fungus Aspergillus niger. 2587. The results ... Soni, N. and Prakash, S. (2013) Possible Mosquito Control by Silver Nanoparticles Synthesized by Soil Fungus (Aspergillus niger ... S. Namita and P. Soam, "Aspergillus niger Metabolites Efficacies against the Mosquito Larval (Culex quinquefasciatus, Anopheles ...
Naringinase from Aspergillus niger was prepared and characterized to evaluate its effectiveness in debittering citrus juice. ... Aspergillus niger / enzymology*. Beverages / analysis*. Citrus / chemistry*. Enzyme Stability. Female. Flavanones / metabolism* ... PRACTICAL APPLICATION: This study focused on characterization, preparation, and validation of naringinase from A. niger, which ...
Effect of Aspergillus Niger Prolyl Endoprotease (AN-PEP) Enzyme on the Effects of Gluten Ingestion in Patients With Coeliac ...
... present investigation deals with the effect of vegetative inoculum on submerged citric acid fermentation by Aspergillus niger ...
Molecular characterization of the secretory pathway of Aspergillus niger. Arthur FJ. Ram, Peter J. Punt, Roy C. Montijn, Cora ... Please note: The Aspergillus Website does not accept advertisments. , all content is published on merit. BB.com. Site Sponsors ... In the first instance we have isolated 9 distinct GTPase encoding genes from A. niger (sarA, sagA-H) corresponding to GTPases ... Aspergillus Website Newsletters. Our most recent newsletters (since August 2018) can be found here. ...
Production, purification and characterization of the catalytic domain of glucoamylase from Aspergillus niger. B Stoffer, T P ... The catalytic domain of glucoamylases G1 and G2 from Aspergillus niger is produced in vitro in high yield by limited ... Production, purification and characterization of the catalytic domain of glucoamylase from Aspergillus niger ... Production, purification and characterization of the catalytic domain of glucoamylase from Aspergillus niger ...
Molecular cloning and heterologous expression of the isopullulanase gene from Aspergillus niger A.T.C.C. 9642. Hiroyoshi AOKI, ... Isopullulanase (IPU) from Aspergillus niger A.T.C.C. (American Type Culture Collection) 9642 hydrolyses pullulan to isopanose. ... Molecular cloning and heterologous expression of the isopullulanase gene from Aspergillus niger A.T.C.C. 9642 ... Molecular cloning and heterologous expression of the isopullulanase gene from Aspergillus niger A.T.C.C. 9642 ...
Kreiner, M. and Harvey, L.M. and McNeil, B. (2003) Morphological and enzymatic responses of a recombinant aspergillus niger to ... Continuous chemostat cultures of a recombinant strain of Aspergillus niger (B1-D), engineered to produce the marker protein hen ... aspergillus niger, oxidative stress, oxygen enrichment, hydrogen peroxide, superoxide dismutase, catalase, Pharmacy and materia ... Morphological and enzymatic responses of a recombinant aspergillus niger to oxidative stressors in chemostat cultures ...
High purity beta-Glucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. ... Native β-Glucosidase from Aspergillus niger. Native β-Glucosidase from Aspergillus niger. ...
Evaluation of Aspergillus niger as host for virus-like particle production, using the hepatitis B surface antigen as a model  ... Location of glucose oxidase during production by Aspergillus niger  Clarke K.G.; Johnstone-Robertson M.; Price B.; Harrison S. ... Expression of the Aspergillus niger glucose oxidase gene in Saccharomyces cerevisiae  Malherbe, Daniel Francois (Stellenbosch ... The filamentous fungus Aspergillus niger was transformed with the hepatitis B virus S gene encoding the major viral envelope ...
Aspergillus niger is a fungus and one of the most common species of the genus Aspergillus. It causes a disease called black ... Abarca M, Bragulat M, Castellá G, Cabañes F (1994). "Ochratoxin A production by strains of Aspergillus niger var. niger". Appl ... Recently the strain of ATCC 16404 Aspergillus niger has been reclassified at Aspergillus brasiliensis (refer to publication by ... niger strains do produce ochratoxin A. It also produces the isoflavone orobol. A. niger is included in Aspergillus subgenus ...
Aspergillus niger CBS 513.88 Aspergillus niger CBS 513.88. Aspergillus niger CBS 513.88 RefSeq Genome ... Aspergillus; Aspergillus niger; Aspergillus niger CBS 513.88 ... Aspergillus niger CBS 513.88. Aspergillus niger CBS 513.88 ... Juhász A et al., "Comparative analysis of the complete mitochondrial genomes of Aspergillus niger mtDNA type 1a and Aspergillus ... The Aspergillus niger reference sequence (RefSeq) genome is provided by the NCBI and is available here. The protein coding ...
Aspergillus mold can be found in soil, foods, fresh water, and other natural sources including compost, barley, tobacco, and ... Aspergillus infection can also result in allergic bronchopulmonary aspergillosis (ABPA), a condition where airway colonization ... 5Aspergillus mold can also grow on ceiling and walls where water damage has occurred.6 ... 7 Testing for Aspergillus precipitating IgG antibodies has been shown to be of some clinical utility in the assessment of ...
Aspergillus,/i, endophthalmitis following Baerveldt glaucoma drainage device implantation with intravitreal and topical ... Infected Baerveldt Glaucoma Drainage Device by Aspergillus niger. Nurul-Laila Salim. ,1. ,. 2 Yaakub Azhany. ,1. ,. 2 Zaidah ... The Aspergillus niger was isolated on the third day based on colony morphology and characteristics features under light ... We report a case of successfully treated infected GDD caused by Aspergillus niger with intravitreal and topical voriconazole ...
Aspergillus niger Study Kit. Item # 155779 *bvseo_sdk, java_sdk, bvseo-4.0.0 ...
Aspergillus niger ATCC ® 16404NA™ Designation: WLRI 034(120) [CBS 733.88, DSM 1387, DSM 1988, IFO 9455, IMI 149007, NCPF 2275] ...
... niger grown on Sabouraud agar medium. 100x magnification. Scientific classification Domain: Eukaryota ... Aspergillus niger. van Tieghem 1867 Aspergillus niger is a fungus and one of the most common species of the genus Aspergillus. ... Abarca M, Bragulat M, Castellá G, Cabañes F (1994). "Ochratoxin A production by strains of Aspergillus niger var. niger". Appl ... A. niger is included in Aspergillus subgenus Circumdati, section Nigri. The section Nigri includes 15 related black-spored ...
MIXED ASPERGILLUS (aspergillus flavus var. oryzae, aspergillus niger var. niger, aspergillus repens, aspergillus terreus) ... FAPP MIXTURE (aspergillus flavus var. oryzae, aspergillus niger var. niger, aspergillus repens, aspergillus terreus, gibberella ... A-22 (aspergillus flavus, aspergillus fumigatus, eurotium herbariorum, aspergillus nidulans, aspergillus niger var. niger, ... oryzae, aspergillus niger var. niger, aspergillus repens, aspergillus terreus, cladosporium sphaerospermum and penicillium ...
For Aspergillus niger the MIC without starch was 51% and 59%, respectively. When starch was incubated with honey and then added ... Additive action of honey and starch against Candida albicans and Aspergillus niger.. Boukraâ L1, Bouchegrane S. ... niger was 40% and 45%, with a starch concentration of 5.6% and 5.1% respectively. This study suggests that the amylase present ...
Cultures of Aspergillus niger NRRL-599 in fluid Sabouraud medium were grown with quinazoline and phthalazine for 7 days. ... Biotransformation of quinazoline and phthalazine by Aspergillus niger.. [John B Sutherland, Thomas M Heinze, Laura K ...
Aspergillus luchuensis CBS 106.47. Aspergillus brasiliensis CBS 101740. Aspergillus tubingensis CBS 134.48. Aspergillus niger ( ... Aspergillus niger. Aspergillus ochraceoroseus IBT 24754. Aspergillus luchuensis. Aspergillus kawachii (strain NBRC 4308) (White ... Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7). Aspergillus ... Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7). ...
Localization of glucose oxidase and catalase activities in Aspergillus niger.. Author(s). Witteveen, C.F.B.; Veenhuis, M.; ...
Im facing problem in counting my Aspergillus Niger. I grew the Aspergillus Niger on Sabouraud dextrose agar with ... The problem is after 5 days incubation, my Aspergillus grows all over the aga... ... how to do Aspergillus Niger count? - posted in Microbiology: Hi, ... how to do Aspergillus Niger count?. Started by dingdong, Apr 04 ... Hi, Im facing problem in counting my Aspergillus Niger. I grew the Aspergillus Niger on Sabouraud dextrose agar with ...
In an article in Nature, CBS-KNAW researchers describe fungus Aspergillus nigers flexibility to turn on the production of ... The ubiquitous fungus Aspergillus niger grows radially, forming long hyphae penetrating the substrate. It is an industrial ... Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization. In: Scientific ... A. niger is able to turn on the production of different enzyme sets in different parts of its colony. Like a Swiss army knife, ...
Aspergillus niger endopolygalacturonase E: a cell degrading enzyme found in microorganisms and higher plants; nucleotide ... Aspergillus niger endopolygalacturonase E. Subscribe to New Research on Aspergillus niger endopolygalacturonase E ... endopolygalacturonase E, Aspergillus niger; pgaE protein, Aspergillus niger. Networked: 0 relevant articles (0 outcomes, 0 ...
sp,A2R3Z3,MDV1_ASPNC Mitochondrial division protein 1 OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mdv1 ... Aspergillus niger (strain CBS 513.88 / FGSC A1513). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_ ...
Background Aspergillus fumigatusis the main causative agent of aspergillosis. Infections rarely occur in immunocompetent ... Other Aspergillus species (e.g. Aspergillus flavus, Aspergillus niger, Aspergillus tubingensis) also cause infections but to a ... Expression profile analysis reveals that Aspergillus fumigatus but not Aspergillus niger makes type II epithelial lung cells ... Other aspergilli like Aspergillus niger also cause infections but to a much lesser extent. Our previous studies showed that A. ...
Phytase Production by Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01 through Submerged and Solid-State Fermentation. ... for the production of phytase from Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01. It was found that both the fungi ... Aspergillus niger CFR 335 and A. ficuum produced a maximum of 60.6 U/gds and 38 U/gds of the enzyme, respectively, in wheat ... niger CFR 335 and A.ficuum, respectively, when grown in potato dextrose broth. ...
Aspergillus nigerlipase immobilization by covalent binding on chitosan-coated magnetic nanoparticles (CMNP), obtained by one- ... Aspergillus niger lipase immobilization by covalent binding on chitosan-coated magnetic nanoparticles (CMNP), obtained by one- ... Immobilization of Aspergillus niger lipase on chitosan-coated magnetic nanoparticles using two covalent-binding methods. ... A. niger lipase Immobilization on magnetic nanoparticles coated with chitosan Glycidol and glutaraldehyde as coupling agents ...
The filamentous fungus Aspergillus niger has extensively been used for recombinant protein production. The sequencing of the A ... Please note: The Aspergillus Website does not accept advertisments. , all content is published on merit. BB.com. Site Sponsors ... Improving A. niger asparaginase via directed evolution Ilse de Lange, Jan-Metske van der Laan, Richard Kerkman, W im Bijleveld ... Aspergillus Website Newsletters. Our most recent newsletters (since August 2018) can be found here. ...
... derived from Aspergillus niger BCRC31494 has been used in the food fermentation industry because of its thermal and alkaline ... Keywords: Aspergillus niger; endoglucanase B; gene cloning and expression; thermal and alkaline tolerance Aspergillus niger; ... Endoglucanase B (EGLB) derived from Aspergillus niger BCRC31494 has been used in the food fermentation industry because of its ... Cloning, Purification, and Characterization of a Heat- and Alkaline-Stable Endoglucanase B from Aspergillus niger BCRC31494. ...
... ... Parthenium hysterophorus and Datura stramonium were evaluated in vitro against Sclerotium rolfsii and Aspergillus niger, the ...
Aspergillus niger - CreA - D-xylose - Dynamic modeling - Parameter estimation - XlnR regulon Abstract. Background: ... Quantitative modeling and analytic assessment of the transcription dynamics of the XlnR regulon in Aspergillus niger. ... Transcription of genes coding for xylanolytic and cellulolytic enzymes in Aspergillus niger is controlled by the transactivator ...
Shotgun Proteomics of Aspergillus niger Microsomes upon d-Xylose Induction. José Miguel P. Ferreira de Oliveira, Mark W. J. van ... Shotgun Proteomics of Aspergillus niger Microsomes upon d-Xylose Induction. José Miguel P. Ferreira de Oliveira, Mark W. J. van ... Shotgun Proteomics of Aspergillus niger Microsomes upon d-Xylose Induction Message Subject (Your Name) has forwarded a page to ... Shotgun Proteomics of Aspergillus niger Microsomes upon d-Xylose Induction. José Miguel P. Ferreira de Oliveira, Mark W. J. van ...
... which is conserved in Aspergillus niger, except for the lra-4 ortholog (lraD). The A. niger cluster also contains the gene ... In vivo functional analysis of L-rhamnose metabolic pathway in Aspergillus niger: a tool to identify the potential inducer of ... In this paper, we confirmed the function of the first three putative L-rhamnose utilisation genes from A. niger through gene ...
  • The cDNA encoding for catalytic subunit of bovine enterokinase (EK(L)), to which the sequence for Kex2 protease cleavage site was inserted, was expressed in the protease deficient filamentous fungus Aspergillus niger AB1.13. (nih.gov)
  • Aspergillus niger is an important fungus, which is widely distributed in nature. (springeropen.com)
  • Aspergillus niger is a filamentous fungus found in the environment, on foods and feeds and is used as host for production of organic acids, enzymes and proteins. (biomedcentral.com)
  • In this process, cellulolytic enzymes were produced by culturing fungus ( Aspergillus niger ) directly on the surface of the biomass. (hud.ac.uk)
  • In the first instance we have isolated 9 distinct GTPase encoding genes from A. niger (sarA, sagA-H) corresponding to GTPases involved in most stages of the secretory pathway. (aspergillus.org.uk)
  • Due to its excellent ability to secrete proteins and organic acids, A. niger is extensively used as an industrial workhorse for glucoamylase, glucose oxidase and citric acid production (Lu et al. (springeropen.com)
  • In this work, the dynamic response of intracellular and extracellular metabolites of Aspergillus niger was investigated after sudden exposure to high and low excess glucose concentrations in chemostats. (springeropen.com)
  • However, a different response of the NAD + /NADH ratio to the glucose pulses was found in A. niger compared to previously published observations on Penicillium chrysogenum and Saccharomyces cerevisiae . (springeropen.com)
  • We have started research to analyse the molecular mechanism underlying protein secretion in A. niger. (aspergillus.org.uk)
  • Continuous chemostat cultures of a recombinant strain of Aspergillus niger (B1-D), engineered to produce the marker protein hen egg white lysozyme, were investigated with regard to their susceptibility to oxidative stress. (strath.ac.uk)
  • A total of 49 protein sequences of alkaline proteases retrieved from GenBank representing different species of Aspergillus have been characterized for various physiochemical properties, homology search, multiple sequence alignment, motif, and super family search and phylogenetic tree construction. (usda.gov)
  • cis-Aconitate decarboxylase (CAD, also known as ACOD1 or Irg1) converts cis-aconitate to itaconate and plays central roles in linking innate immunity with metabolism and in the biotechnological production of itaconic acid by Aspergillus terreus We have elucidated the crystal structures of human and murine CADs and compared their enzymological properties to CAD from A. terreus Recombinant CAD is fully active in vitro without a cofactor. (helmholtz-hzi.de)
  • Molecular characterization of the secretory pathway of Aspergillus niger. (aspergillus.org.uk)
  • Production of a few other A. niger secondary metabolites was affected similarly by lactate and starch (fumonisin B 4 , orlandin, desmethylkotanin and pyranonigrin A), while production of others was not (ochratoxin A, ochratoxin alpha, malformin A, malformin C, kotanin, aurasperone B and tensidol B). The proteome of A. niger was clearly different during growth on media containing 3% starch, 3% starch + 3% lactate or 3% lactate. (biomedcentral.com)
  • The mycotoxin fumonisin B 2 was recently found to be produced by A. niger and hence very little is known about production and regulation of this metabolite. (biomedcentral.com)
  • Proteome analysis was used with the purpose to reveal how fumonisin B 2 production by A. niger is influenced by starch and lactate in the medium. (biomedcentral.com)
  • Fumonisin B 2 production by A. niger was significantly increased when lactate and starch were combined in the medium. (biomedcentral.com)
  • Lactate added in a medium containing nitrate and starch can increase fumonisin B 2 production by A. niger as well as production of some other secondary metabolites. (biomedcentral.com)
  • The results lead to the hypothesis that fumonisin production by A. niger is regulated by acetyl-CoA. (biomedcentral.com)
  • Due to the ubiquity of A. niger , its production of secondary metabolites is important both from a biotechnological and a food-safety viewpoint. (biomedcentral.com)
  • The secreted EK(L) was easily purified from other proteins found in A. niger culture supernatant, using ion exchange and affinity chromatography. (nih.gov)
  • The highly enantioselective epoxide hydrolase from Aspergillus niger is well utilized as biocatalysts for the preparation of enantiopure chiral epoxides and diols. (elsevier.com)
  • The annotation has been compared with that submitted to GenBank and the results presented at Asperfest 8 with an abstract (Alternative annotation of Aspergillus genomes with multi-genome Gnomon method) available here . (nih.gov)
  • abstract = "Aspergillus niger ORS-4.410, a mutant of A. niger ORS-4, was generated by repeated ultraviolet (UV) irradiation. (elsevier.com)
  • We have isolated the ipuA gene encoding IPU from the filamentous fungi A. niger A.T.C.C. 9642. (biochemj.org)
  • One wild-type strain and eight mutants of the fungi species of Aspergillus niger were screened for their ability to produce oxalic acid on lipids media using batch fermentation. (media.pl)
  • Thus, this study focused on isolation and activity determination of pectinases and amylases secreted by the fungi Aspergillus niger. (begellhouse.com)
  • The ability of many filamentous fungi, such as Aspergillus niger, to secret a high level of homologous proteins has led to their consideration as hosts for the production of heterologous proteins. (nottingham.ac.uk)
  • The gold-standard genome of Aspergillus niger NRRL 3 enables a detailed view of the diversity of sugar catabolism in fungi. (doe.gov)
  • An increased understanding of the molecular mechanisms controlling carbon flux in fungi will be gained from study of the A. niger genome. (doe.gov)
  • Contraindications: Do not administer in cases of known hypersensitivity to mold fungi (Aspergillus niger). (iplanethealth.com)
  • It has been well documented that pectinolytic enzymes produced by certain sprouting strains of fungi like Aspergillus niger, Aspergillus oryzae and Rhizopus oryzae have been utilized in fruit clarification process because of their desirable characteristics. (com.ng)
  • A. niger is also cultured for the extraction of the enzyme, glucose oxidase, used in the design of glucose biosensors, due to its high affinity for β-D-glucose. (wikipedia.org)
  • In an article in Nature, CBS-KNAW researchers describe fungus Aspergillus niger 's flexibility to turn on the production of different enzyme sets. (knaw.nl)
  • A. niger is able to turn on the production of different enzyme sets in different parts of its colony. (knaw.nl)
  • Aspergillus niger CFR 335 and A. ficuum produced a maximum of 60.6 U/gds and 38 U/gds of the enzyme, respectively, in wheat bran solid substrate medium. (hindawi.com)
  • A maximum of 9.6 and 8.2 U/mL of enzyme activity was observed in SmF by A. niger CFR 335 and A.ficuum , respectively, when grown in potato dextrose broth. (hindawi.com)
  • The sequencing of the A. niger CBS513-88 genome, an ancestor of our current enzyme production strains, yielded easy access to numerous protein encoding genes. (aspergillus.org.uk)
  • One of those new products is A. niger asparaginase, an enzyme effective in mitigation of acrylamide formation in food products. (aspergillus.org.uk)
  • To improve efficiency of A. niger asparaginase in application, we applied directed evolution to optimize the pH-activity profile of the enzyme. (aspergillus.org.uk)
  • In the present study the investigators wish to determine if co-administration of such an enzyme, a prolyl endoprotease derived from the food grade organism Aspergillis niger (AN-PEP), is capable of detoxifying 8 grams of gluten in a commercial food product. (clinicaltrials.gov)
  • In this study, we analyzed the effects of the d -xylose induction of cellulase and hemicellulase enzyme secretion on the protein composition of secretory organelles in Aspergillus niger . (asm.org)
  • For this reason, A. niger has been used intensively as a cell factory for enzyme production ( 3 , 14 , 32 ). (asm.org)
  • In a previous study, we established defined culture conditions for the induced expression of the cellulase and hemicellulase enzyme system of A. niger ( 56 ). (asm.org)
  • Present investigation involves studies on the effect of carbon and nitrogen sources, temperature and pH on the production of phytase enzyme by Aspergillus niger in submerged fermentation. (scirp.org)
  • Supplementary Fig. 2-Analysis of transformants of Aspergillus niger by agarose gel electrophoresis using PCR products digested by the restriction enzyme Hin dIII. (springer.com)
  • The aim of this study was to compare the frequency of sensitization to Aspergillus xylanase, cellulase, and glucoamylase with the sensitization to alpha-amylase (Asp o 2) and to identify IgE-reactive proteins in enzyme preparations. (ehesp.fr)
  • Sensitization to Aspergillus-derived enzymes and cross-reactivity were retrospectively studied by enzyme allergosorbent test (EAST) and EAST-inhibition experiments. (ehesp.fr)
  • High purity beta-Glucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (creative-enzymes.com)
  • The production of the enzyme glucose oxidase by Aspergillus niger is well documented. (sun.ac.za)
  • High purity α-Glucosidase (transglucosidase) ( Aspergillus niger ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (tms-lab.com)
  • A. niger was resuscitated on potato dextrose agar and then subcultured in medium specific for the production of each targeted enzyme. (begellhouse.com)
  • High purity recombinant endo- Inulinase ( Aspergillus niger ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • Singh, OV 2006, ' Mutagenesis and analysis of mold Aspergillus niger for extracellular glucose oxidase production using sugarcane molasses ', Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology , vol. 135, no. 1, pp. 43-58. (elsevier.com)
  • High purity recombinant exo -Inulinase ( Aspergillus niger ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88. (microbiologyresearch.org)
  • The gene set identified in this manuscript will be highly useful in the annotation of the genome sequence of A. niger , the genes described in the manuscript, especially those encoding hydrolytic enzymes will provide a valuable source for researchers interested in enzyme properties and applications. (biomedcentral.com)
  • IMSEAR at SEARO: Anthranilate hydroxylase, an iron enzyme, from Aspergillus niger. (who.int)
  • The fungus Aspergillus niger var. (springer.com)
  • The cDNA encoding for catalytic subunit of bovine enterokinase (EK(L)), to which the sequence for Kex2 protease cleavage site was inserted, was expressed in the protease deficient filamentous fungus Aspergillus niger AB1.13. (nih.gov)
  • Here, we have synthesized the silver nanoparticles (AgNPs) by using the soil fungus Aspergillus niger 2587. (scirp.org)
  • The ubiquitous fungus Aspergillus niger grows radially, forming long hyphae penetrating the substrate. (knaw.nl)
  • The filamentous fungus Aspergillus niger has extensively been used for recombinant protein production. (aspergillus.org.uk)
  • With the availability of the genome sequence of the filamentous fungus Aspergillus niger, the use of targeted genetic modifications has become feasible. (dtu.dk)
  • The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. (biomedcentral.com)
  • The genome of the industrially important fungus Aspergillus niger encodes a large number of glycoside hydrolase family 18 members annotated as chitinases. (microbiologyresearch.org)
  • Conserved regions within the M1 family of metallo-aminopeptidases have been used to clone a zinc aminopeptidase from the industrially used fungus Aspergillus niger. (openaire.eu)
  • Citric acid production by the filamentous ascomycete fungus, Aspergillus niger represents the most efficient, highest yielding bioprocess in practice. (doe.gov)
  • is produced from the mold fungus Aspergillus niger. (iplanethealth.com)
  • Using identical conditions with a different strain of A. niger , we applied shotgun proteomics for the analysis of enriched microsomal fractions containing endoplasmic reticulum (ER) membranes with associated ribosomes and Golgi membranes. (asm.org)
  • The gluconic acid batch fermentation was conducted using mutant Aspergillus niger NCIM 530 strain under submerged condition in 50 L semiautomatic stirred-tank fermenter. (omicsonline.org)
  • Purane NK, Sharma SK, Salunkhe PD, Labade DS, Tondlikar MM (2012) Gluconic Acid Production from Golden Syrup by Aspergillus niger Strain Using Semiautomatic Stirred-Tank Fermenter. (omicsonline.org)
  • In present work, Aspergillus niger strain (own collection) was used. (scirp.org)
  • The highest amount of ellagitannase (5176.81 U/l) was obtained at 8 h of culture when cranberry polyphenols and strain A. niger PSH were used. (scielo.org.ar)
  • It was observed that the best source for releasing ellagic acid was pomegranate polyphenols and A. niger HT4 strain, which has the ability to degrade these compounds for obtaining a potent bioactive molecule such as ellagic acid. (scielo.org.ar)
  • We expressed two of these enzymes in a suitable A. niger strain and characterized the purified proteins. (asm.org)
  • An agtA knockout of A. niger showed an increased susceptibility towards the cell wall-disrupting compound calcofluor white, indicating a cell wall integrity defect in this strain. (asm.org)
  • The recombinant AB strain contained the α-galactosidase aglB gene from A. niger with its native AglB signal peptide regulated by the glucoamylase promoter. (springer.com)
  • Li J, Zhang H, Zhang Y, Shuang B, Wang D, Zhao N et al (2013) Construction of food-grade xylanase engineering strain of Aspergillus niger . (springer.com)
  • Continuous chemostat cultures of a recombinant strain of Aspergillus niger (B1-D), engineered to produce the marker protein hen egg white lysozyme, were investigated with regard to their susceptibility to oxidative stress. (strath.ac.uk)
  • A recombinant Aspergillus niger strain expressing the Hypocrea jecorina endoglucanase Cel7B was grown on spent hydrolysates (stillage) from sugarcane bagasse and spruce wood. (sun.ac.za)
  • The spent hydrolysates served as excellent growth media for the Cel7B-producing strain, A. niger D15[egI], which displayed higher endoglucanase activities in the spent hydrolysates than in standard medium with a comparable monosaccharide content (e.g., 2,100 nkat/ml in spent bagasse hydrolysate compared to 480 nkat/ml in standard glucose-based medium). (sun.ac.za)
  • Although the transcription of glaA was attenuated in that strain of A. niger, UPR was not evident, suggesting that the transcriptional down-regulation mechanism is controlled differently from the UPR. (nottingham.ac.uk)
  • Here we report the rewiring of a secondary metabolite pathway towards further improved IA production through the overexpression of a putative cytosolic citrate synthase citB in a A. niger strain carrying the IA biosynthesis cluster. (biomedcentral.com)
  • Expression of the IA biosynthesis cluster in Aspergillus niger AB1.13 strain enables IA production. (biomedcentral.com)
  • The level of glucose oxidase (GOX) production from mutant A. niger ORS-4.410 thus obtained was 149% higher than that for WT strain A. niger ORS-4 under liquid culture conditions using hexacyanoferrate (HCF)-treated sugarcane molasses (TM) as a cheaper carbohydrate source. (elsevier.com)
  • Mutant A. niger ORS-4.410 was markedly different from the parent strain morphologically and was found to grow abundantly on sugarcane molasses. (elsevier.com)
  • Production of the 24-kDa S protein and a 48-kDa S protein dimer in the membrane-associated protein fraction of the recombinant A. niger strain was shown through Western analysis. (sun.ac.za)
  • Together, these data reveal the existence of a distinct strain of A. niger on board the ISS and provide insight into the characteristics of melanized fungal species inhabiting spacecraft environments. (cdc.gov)
  • de Vries RP, van den Broeck HC, Dekkers E, Manzanares P, de Graaff LH, Visser J (1999) Differential expression of three α-galactosidase genes and a single β-galactosidase gene from Aspergillus niger. (springer.com)
  • Comparative analysis of the complete mitochondrial genomes of Aspergillus niger mtDNA type 1a and Aspergillus tubingensis mtDNA type 2b. (nih.gov)
  • In conclusion, induction of extracellular enzymes results in specific changes in the secretory subproteome of A. niger , and the most prominent change found in this study was the recruitment of the 20S proteasomal subunits to the secretory organelles. (asm.org)
  • A high level of synteny was observed between A. niger and other sequenced aspergilli, although more extracellular hydrolytic enzymes were annotated for A. niger . (asm.org)
  • Aspergillus niger NRRL330 produces extracellular β-fructofuranosidase (Ffase), and its production is subject to repression by hexoses in the medium. (ovid.com)
  • Singh, O. V. / Mutagenesis and analysis of mold Aspergillus niger for extracellular glucose oxidase production using sugarcane molasses . (elsevier.com)
  • Aspergillus niger is extensively used for the industrial production of organic acids and extracellular enzymes. (doe.gov)
  • The secretory potential of A. niger is not well understood, and only a limited number of functional studies have been performed to investigate the major components of the fungal secretion pathway. (asm.org)
  • 8] and Raukas [13] reported that addition of 1- 6 % methanol resulted in a marked increase in the amount of citric acid formed by A. niger on fruits waste and the carob pod extracts, respectively. (media.pl)
  • A 2526 bp gene encoding Aspergillus niger Beta-glucosidase was chemically synthesized for its heterologous expression in methylotrophic yeast Pichia pastoris , using methanol as inducer. (ccsenet.org)
  • The production level of hepatitis B pseudoviral particles was estimated to be 0.4 mg/1 culture, which compares favourably with the reported levels initially obtained in yeast, indicating the potential of the Aspergillus expression system as an alternative, cost-effective vaccine production system. (sun.ac.za)
  • The cfcI expression profile suggests that its physiological function is important in processes that take place during the late stages of the aspergillus life cycle, such as autolysis or sporulation. (microbiologyresearch.org)
  • Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. (nih.gov)
  • Recently, the full genome sequence of A. niger CBS 513.88 was determined and annotated ( 55 ). (asm.org)
  • There are many data in literature describing biosynthesis of oxalic acid only on such carbohydrates as sucrose, lactose, maltodextrin and xylose by A. niger . (media.pl)
  • In our previous work we showed that post-refining fatty acid (a by-product of plant oil production) was the efficient substrate for the biosynthesis of oxalic acid by a mutant of A. niger [14]. (media.pl)
  • Our previous work showed, that raw lipid materials can be regarded as efficient substrates for the biosynthesis of oxalic acid by A. niger at low pH rang ed from 4 to 5 [16, 17]. (media.pl)
  • The results suggest that A. niger could directly inhibit AFB1 biosynthesis through reducing the abundance of aflS to aflR mRNAs. (usda.gov)
  • A. niger is cultured for the industrial production of many substances. (wikipedia.org)
  • Another use for A. niger within the biotechnology industry is in the production of magnetic isotope-containing variants of biological macromolecules for NMR analysis. (wikipedia.org)
  • Ochratoxin A production by strains of Aspergillus niger var. (wikipedia.org)
  • In this study, a comparison was made between submerged (SmF) and solid-state fermentations (SSF) for the production of phytase from Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01. (hindawi.com)
  • The three recombinant chymosins that were reviewed in this monograph, as well as their respective production organisms are identified below: (1) chymosin A from Escherichia coli K-12 (2) chymosin B from Kluyveromyces lactis, and (3) chymosin B from Aspergillus niger var. (inchem.org)
  • Among the inorganic and organic nitrogen sources, ammonium nitrate in concentration of 0.5% was found to be a favorable nitrogen source for phytase production in Aspergillus niger. (scirp.org)
  • Optimum temperature and pH for phytase production by Aspergillus niger were 30˚C and 5.5. (scirp.org)
  • The fermentation substrate efficiency of glucose supplemented with guava seed flour (GGSF) or glucose supplemented with dry Aspergillus niger mycelium (GANM) was evaluated during the production of biomass and antimicrobial compounds by the lactic acid bacteria Weissella confusa . (ejbiotechnology.info)
  • The aim of this study was to construct recombinant Aspergillus niger strains with high α-galactosidase production. (springer.com)
  • Aleksieva P, Tchorbanov B, Nacheva L (2010) High-yield production of alpha-galactosidase excreted from Penicillium chrysogenum and Aspergillus niger . (springer.com)
  • Using molecular biology techniques, this study focused on metabolic engineering of A. niger to manipulate its organic acid production in the direction of succinic acid. (dtu.dk)
  • Deletion of the acl led to increased succinic acid production by A. niger. (dtu.dk)
  • A. niger XP (induced with UV irradiation) was found to be the most suitable for oxalic acid production from lipid substrates. (media.pl)
  • Thus, oxalic acid production by A. niger can be favored maintaining a high pH, ranging from 6 to 7, when the culture medium is enriched with appropriate amounts of nitrogen and phosphorus. (media.pl)
  • Up till now, the renewable products such as beet molasses, a green corn syrup and milk whey were used for oxalic acid production by A. niger [4, 8]. (media.pl)
  • In this report, the results of the screening of 9 fungal cultures A. niger for oxalic acid production from crude rapeseed oil are discussed. (media.pl)
  • The stimulating effects of methanol on the growth and production of oxalic acid from post-refining fatty acids (by-product of plant oil production containing free fatty acids) by a mutant of Aspergillus niger were investigated in submerged fermentation experiments. (media.pl)
  • Methanol is not assimilated by A. niger and its exact role in stimulating the production of citric or gluconic acid by A. niger is still not clear [7, 9]. (media.pl)
  • A. niger produces oxalic acid as a by-product which causes problems with downstream processing of industrial production of citric acid. (media.pl)
  • Production of oxalic acid from lipids by A. niger in the presence of methanol has not been investigated up till now. (media.pl)
  • The purpose of the present investigation was to determine the stimulating value of methanol on the production of oxalic acid from post-refining fatty acids by A. niger . (media.pl)
  • have previously proposed Aspergillus niger as a suitable production host for the industrial production of IA due to the hosts optimized pathways towards organic acids. (biomedcentral.com)
  • Twenty Aspergillus niger strains were isolated from peanuts and 14 strains were able to completely inhibit AFB1 production with co-cultivation. (usda.gov)
  • The secondary substrate binding site (SBS) of Bacillus subtilis and Aspergillus niger glycoside hydrolase family 11 xylanases was studied by site-directed mutagenesis and evaluation of activity and binding properties of mutant enzymes on different substrates. (kuleuven.be)
  • This feature has been exploited in biotechnology to establish platform strains with high secretory capacity including Aspergillus niger . (biomedcentral.com)
  • Bacillus subtilis attachment to Aspergillus niger hyphae results in mutually altered metabolism. (semanticscholar.org)
  • Methanol has a profound effect on the metabolism of sugars by A. niger . (media.pl)
  • Metabolism of DL-(+/-)-phenylalanine by Aspergillus niger. (iisc.ernet.in)
  • Aspergillus niger is presently one of the most important organisms used in biotechnology. (biomedcentral.com)
  • Occupational IgE sensitisation to phytase, a phosphatase derived from Aspergillus niger. (bmj.com)
  • OBJECTIVE: Phytase is a phosphatase derived from Aspergillus niger that enhances phosphate bioavailability in the gut, and therefore has been increasingly used as an animal feed additive since the early 1990s. (bmj.com)
  • Maximal phytase activity of Aspergillus niger was detected in media with 1.0% sucrose as a carbon source. (scirp.org)
  • Micrograph of A. niger grown on Sabouraud agar medium. (bionity.com)
  • Cultures of Aspergillus niger NRRL-599 in fluid Sabouraud medium were grown with quinazoline and phthalazine for 7 days. (sigmaaldrich.com)
  • Three types of polysaccharides (high, low and intermediate molecular weight), non covalently attached to glucosidase, were observed when Aspergillus niger NIAB 280 was grown on kallar gray wheat bran and salicin in combination. (gov.pk)
  • Summary: The activities of β-oxidation enzymes were measured in extracts of glucose-and triolein-grown cells of Aspergillus niger. (microbiologyresearch.org)
  • Important for an economically competitive fermentation process is the ability of A. niger to grow and to produce oxalic acid on low cost carbon substrates as renewable products from agriculture and food industry. (media.pl)
  • Among all the solvent extracts, methanol extract of P. hysterophorus had potential antifungal activity against S. rolfsii and A. niger . (cabi.org)
  • Exo-Polygalacturonase (Exo-PG) produced from a newly isolated Aspergillus niger AUMC 4156 in fermentation media containing sugar beet pulp as a sole carbon source was separated and purified by filtration, ammonium sulfate precipitation, dialysis, cold acetone precipitation and sephadex G-200 gel filtration chromatography. (omicsonline.org)
  • and molasses and dry mycelium of Aspergillus niger (MMAN). (scielo.org.co)
  • According to Adham [1], olive oil at 4 % concentration added to beet molasses caused a considerable increase in citric acid yield from A. niger . (media.pl)
  • CANDIDA (ammoniac, aspergillus niger var. (nih.gov)
  • Additive action of honey and starch against Candida albicans and Aspergillus niger. (nih.gov)
  • Genome-wide analysis was performed to assess the transcriptional landscape of germinating A. niger conidia using both next generation RNA-sequencing and GeneChips. (scoop.it)
  • As a preliminary attempt to investigate if the transcriptional downregulation effect was mediated through HacA (i.e. part of the UPR), the ER stress was induced through antisense technology to lower the level of POI in the ER of A. niger. (nottingham.ac.uk)
  • Furthermore, activation of the ER-Associated Degradation (ERAO) mechanism in OTT-treated A. niger cultures was demonstrated by detecting transcriptional up-regulation of the putative gene encoding the RpnG, a homologue of the yeast Rpn7p subunit of the 26S proteasome. (nottingham.ac.uk)
  • I grew the Aspergillus Niger on Sabouraud dextrose agar with chloramphenicol(SDAC), incubated for 5 days. (protocol-online.org)
  • acetone, chloroform, distilled water, ethanol and methanol extractions of two weeds namely, Parthenium hysterophorus and Datura stramonium were evaluated in vitro against Sclerotium rolfsii and Aspergillus niger , the causal agents of collar rot and seed rot of chickpea, respectively. (cabi.org)