Aspergillus
Aspergillus fumigatus
Aspergillus nidulans
Aspergillus flavus
Glucan 1,4-alpha-Glucosidase
6-Phytase
Polygalacturonase
Fungi
Antifungal Agents
Citric Acid
Penicillium
Gene Expression Regulation, Fungal
Xylosidases
Glucose Oxidase
Molecular Sequence Data
Mycobacillin
Industrial Microbiology
Hydrogen-Ion Concentration
Hyphae
Aflatoxins
Fermentation
Ochratoxins
Aspergillus ochraceus
Starch
Endo-1,4-beta Xylanases
Amino Acid Sequence
Culture Media
Carboxylic Ester Hydrolases
Substrate Specificity
Cellulase
Mitosporic Fungi
Maltose
Food Preservatives
Pectins
Pulmonary Aspergillosis
Rhizopus
Itraconazole
Aspergillosis, Allergic Bronchopulmonary
beta-Glucosidase
Sterigmatocystin
Enzyme Stability
Drug Resistance, Fungal
Mycology
Cloning, Molecular
Lung Diseases, Fungal
beta-Mannosidase
Transformation, Genetic
Trichoderma
Xylan Endo-1,3-beta-Xylosidase
Microbial Sensitivity Tests
Base Sequence
Aspergillus oryzae
Amphotericin B
Temperature
Invasive Pulmonary Aspergillosis
Purification and characterization of methylamine oxidase induced in Aspergillus niger AKU 3302. (1/718)
Crude extract of Aspergillus niger AKU 3302 mycelia incubated with methylamine showed a single amine oxidase activity band in a developed polyacrylamide gel that weakly cross-reacted with the antibody against a copper/topa quinone-containing amine oxidase (AO-II) from the same strain induced by n-butylamine. Since the organism cannot grow on methylamine and the already known quinoprotein amine oxidases of the organism cannot catalyze oxidation of methylamine, the organism was forced to produce another enzyme that could oxidize methylamine when the mycelia were incubated with methylamine. The enzyme was separated and purified from the already known two quinoprotein amine oxidases formed in the same mycelia. The purified enzyme showed a sharp symmetric sedimentation peak in analytical ultracentrifugation showing S20,w0 of 6.5s. The molecular mass of 133 kDa estimated by gel chromatography and 66.6 kDa found by SDS-PAGE confirmed the dimeric structure of the enzyme. The purified enzyme was pink in color with an absorption maximum at 494 nm. The enzyme readily oxidized methylamine, n-hexylamine, and n-butylamine, but not benzylamine, histamine, or tyramine, favorite substrates for the already known two quinoprotein amine oxidases. Inactivation by carbonyl reagents and copper chelators suggested the presence of a copper/topa quinone cofactor. Spectrophotometric titration by p-nitrophenylhydrazine showed one reactive carbonyl group per subunit and redox-cyclic quinone staining confirmed the presence of a quinone cofactor. pH-dependent shift of the absorption spectrum of the enzyme-p-nitrophenylhydrazone (469 nm at neutral to 577 nm at alkaline pH) supported the identity of the cofactor with topaquinone. Nothern blot analysis indicated that the methylamine oxidase encoding gene is largely different from the already known amine oxidase in the organism. (+info)Citric acid production from xylan and xylan hydrolysate by semi-solid culture of Aspergillus niger. (2/718)
Citric acid production from xylan and xylan hydrolysate was done by Aspergillus niger Yang no. 2 cultivated in a semi-solid culture using bagasse as a carrier. Yang no. 2 produced 72.4 g/l and 52.6 g/l of citric acid in 5 d from 140 g/l of xylose and arabinose, respectively. Yang no. 2 produced 51.6 g/l of citric acid in 3 d from a concentrated xylan hydrolysate prepared by cellulase treatment, containing 100 g/l of reducing sugars. Moreover, Yang no. 2 directly produced 39.6 g/l of citric acid maximally in 3 d from 140 g/l of xylan. (+info)Production of specific monoclonal antibodies to Aspergillus species and their use in immunohistochemical identification of aspergillosis. (3/718)
Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining. (+info)Kinetic characterization of Aspergillus niger N400 endopolygalacturonases I, II and C. (4/718)
Endopolygalacturonases I, II and C isolated from recombinant Aspergillus niger strains were characterized with respect to pH optimum, activity on polygalacturonic acid and mode of action and kinetics on oligogalacturonates of different chain length (n = 3-7). Apparent Vmax values using polygalacturonate as a substrate at the pH optimum, pH 4.1, were calculated as 13.8 mukat.mg-1, 36.5 mukat.mg-1 and 415 nkat.mg-1 for endopolygalacturonases I, II and C, respectively. K(m) values were < 0.15 mg.mL-1 for all three enzymes. Product progression analysis using polygalacturonate as a substrate revealed a random cleavage pattern for all three enzymes and suggested processive behavior for endopolygalacturonases I and C. This result was confirmed by analysis of the mode of action using oligogalacturonates. Processivity was observed when the degree of polymerization of the substrate exceeded 5 or 6 for endopolygalacturonase I and endopolygalacturonase C, respectively. The bond-cleavage frequencies obtained for the hydrolysis of the oligogalacturonates were used to assess subsite maps. The maps indicate that the minimum number of subsites is seven for all three enzymes. Using pectins of various degrees of esterification, it was shown that endopolygalacturonase II is the most sensitive to the presence of methyl esters. Like endopolygalacturonase II, endopolygalacturonases I, C and E, which was also included in this part of the study, preferred the non-esterified pectate. Additional differences in substrate specificity were revealed by analysis of the reaction products of hydrolysis of a mixture of pectate lyase-generated delta 4,5-unsaturated oligogalacturonates of degree of polymerization 4-8. Whereas endopolygalacturonase I showed a strong preference for generating the delta 4,5-unsaturated dimer, with endopolygalacturonase II the delta 4,5-unsaturated trimer accumulated, indicating further differences in substrate specificity. For endopolygalacturonases C and E both the delta 4,5-unsaturated dimer and trimer were observed, although in different ratios. (+info)Possibility for discriminating between two representative non two-state thermal unfolding models of proteins by DSC. (5/718)
Possible differences between two representative non two-state thermal unfolding mechanisms of protein are discussed concerning differential scanning calorimetry. Numerical simulations showed that, by DSC measurement, it is hard to discriminate between the independent model, which assumes independent unfolding domains in a protein, and the sequential model, which assumes intermediate(s) between native and denatured states, especially when values of molecular weight, denaturation enthalpy, and difference in denaturation temperature of each denaturation process are large. DSC curve analysis of Aspergillus niger glucoamylase based on these two models gave essentially the same thermodynamic parameters. (+info)Pseudoepidemic of Aspergillus niger infections traced to specimen contamination in the microbiology laboratory. (6/718)
We report a pseudo-outbreak of Aspergillus niger that followed building construction in our clinical microbiology laboratory. Because outbreaks of invasive aspergillosis have been linked to hospital construction, strategies to minimize dust in patient care areas are common practice. We illustrate that the impact of false-positive cultures on patient care should compel laboratories to prevent specimen contamination during construction. (+info)Oxidation of medium-chain acyl-CoA esters by extracts of Aspergillus niger: enzymology and characterization of intermediates by HPLC. (7/718)
The activities of beta-oxidation enzymes were measured in extracts of glucose- and triolein-grown cells of Aspergillus niger. Growth on triolein stimulated increased enzyme activity, especially for acyl-CoA dehydrogenase. No acyl-CoA oxidase activity was detected. HPLC analysis after incubation of triolein-grown cell extracts with decanoyl-CoA showed that beta-oxidation was limited to one cycle. Octanoyl-CoA accumulated as the decanoyl-CoA was oxidized. Beta-oxidation enzymes in isolated mitochondrial fractions were also studied. The results are discussed in the context of methyl ketone production by fungi. (+info)Biotransformation of (1-phenyl)ethyl hydroperoxide with Aspergillus niger: a model study on enzyme selectivity and on the induction of peroxidase activity. (8/718)
The biocatalytic enantioselective reduction of (1-phenyl)ethyl hydroperoxide (1) by the fungus Aspergillus niger to the corresponding alcohol 2 involves a multi-enzyme biotransformation of the hydroperoxide 1, as revealed by the change in the enantioselectivity as a function of incubation times. This unusual behavior is not exhibited by other fungi and seems to be restricted to A. niger. Furthermore, the peroxidase and other oxidoreductase activities of A. niger depend on the availability of metal ions such as Fe2+, Mn2+ and Zn2+ in the growth medium, since the addition of Fe2+ ions substantially (threefold) increases the enantioselectivity, whereas addition of Mn2+ and Zn2+ ions decreases it. Finally, the cold shock (4 degrees C) significantly enhances the reduction of the hydroperoxide by the microorganism A. niger. (+info)The symptoms of aspergillosis depend on the location and severity of the infection. In the lungs, it may cause coughing, fever, chest pain, and difficulty breathing. In the sinuses, it can cause headaches, facial pain, and nasal congestion. In the brain, it can cause seizures, confusion, and weakness.
Aspergillosis is typically diagnosed through a combination of imaging tests such as chest X-rays, CT scans, and MRI scans, along with a biopsy to confirm the presence of Aspergillus fungi.
Treatment of aspergillosis depends on the severity and location of the infection. In mild cases, treatment may involve antifungal medications and supportive care such as oxygen therapy and pain management. In severe cases, treatment may require hospitalization and intravenous antifungal medications.
Preventive measures for aspergillosis include avoiding exposure to dusty or damp environments, managing chronic conditions such as asthma and COPD, and taking antifungal medications as prescribed.
Aspergillosis can be a serious condition, especially in people with weakened immune systems, such as those with cancer, HIV/AIDS, or taking immunosuppressive drugs. In severe cases, aspergillosis can lead to life-threatening complications such as respiratory failure, sepsis, and organ damage.
In conclusion, aspergillosis is a common fungal infection that can affect various parts of the body, and it can be serious and potentially life-threatening, especially in people with weakened immune systems. Early diagnosis and appropriate treatment are essential to prevent complications and improve outcomes.
Pulmonary aspergillosis is a type of fungal infection that affects the lungs and is caused by the fungus Aspergillus. It can occur in people with weakened immune systems, such as those with cancer, HIV/AIDS, or taking immunosuppressive drugs following an organ transplant.
The symptoms of pulmonary aspergillosis can vary depending on the severity of the infection and may include:
* Coughing up blood or mucus
* Chest pain or tightness
* Fever
* Shortness of breath
* Chills
* Weight loss
In severe cases, pulmonary aspergillosis can lead to respiratory failure, which can be life-threatening.
Pulmonary aspergillosis is diagnosed through a combination of imaging tests such as chest X-rays, CT scans, and fungal cultures. Treatment typically involves antifungal medications and supportive care to manage symptoms and prevent complications. In severe cases, hospitalization may be necessary to provide oxygen therapy and other respiratory support.
Prevention is key in avoiding pulmonary aspergillosis, especially for individuals with weakened immune systems. This includes avoiding exposure to fungal spores, managing underlying health conditions, and taking antifungal medications as prescribed. Early diagnosis and treatment can significantly improve outcomes for those affected by this condition.
The main cause of ABPA is exposure to airborne spores of the fungus Aspergillus, which are commonly found in soil and decaying organic matter. Individuals with a pre-existing allergic condition may be more susceptible to developing an allergic reaction to these spores, leading to inflammation and damage to the airways.
Diagnosis of ABPA typically involves a combination of physical examination, medical history, and diagnostic tests such as chest X-rays, CT scans, and bronchoscopy with biopsy. Treatment for ABPA typically involves corticosteroids to reduce inflammation and antifungal medications to treat any underlying infection. In severe cases, hospitalization may be necessary to provide oxygen therapy and other supportive care.
Prevention of ABPA includes avoiding exposure to known allergens and maintaining good respiratory hygiene. This can involve regularly cleaning and disinfecting surfaces and objects, using HEPA filters in air purifiers, and wearing a mask when working with or around potentially contaminated materials.
Prognosis for ABPA is generally good if treated promptly and effectively, but untreated cases can lead to serious complications such as respiratory failure and other organ damage. With proper management and prevention strategies in place, individuals with ABPA can lead active and fulfilling lives.
Types of fungal lung diseases include:
1. Aspergillosis: This is an infection caused by the fungus Aspergillus, which is commonly found in soil and decaying organic matter. It can affect people with weakened immune systems, such as those with cancer, HIV/AIDS, or taking immunosuppressive drugs.
2. Cryptococcosis: This is an infection caused by the fungus Cryptococcus neoformans, which is found in soil and decaying wood. It can affect people with weakened immune systems, such as those with HIV/AIDS or taking immunosuppressive drugs.
3. Histoplasmosis: This is an infection caused by the fungus Histoplasma capsulatum, which is found in soil and decaying organic matter. It can affect people with weakened immune systems, such as those with HIV/AIDS or taking immunosuppressive drugs.
4. Pneumocystis pneumonia (PCP): This is an infection caused by the fungus Pneumocystis jirovecii, which is found in soil and decaying organic matter. It can affect people with weakened immune systems, such as those with HIV/AIDS or taking immunosuppressive drugs.
5. Sporotrichosis: This is an infection caused by the fungus Sporothrix schenckii, which is found in soil and decaying organic matter. It can affect people with weakened immune systems, such as those with HIV/AIDS or taking immunosuppressive drugs.
Symptoms of fungal lung diseases can include:
* Cough
* Fever
* Chest pain
* Shortness of breath
* Fatigue
Diagnosis of fungal lung diseases is typically made through a combination of physical examination, medical history, and laboratory tests such as chest X-rays, CT scans, and fungal cultures. Treatment usually involves antifungal medications and may also include supportive care to manage symptoms.
Prevention of fungal lung diseases includes:
1. Avoiding exposure to fungal spores by wearing protective clothing and gear when working with soil or decaying organic matter.
2. Maintaining good indoor air quality by using ventilation systems and reducing humidity.
3. Reducing the risk of infection by avoiding close contact with people who are at high risk of developing fungal lung diseases, such as those with weakened immune systems.
4. Avoiding smoking and other tobacco products, which can increase the risk of developing fungal lung diseases.
5. Managing underlying medical conditions, such as HIV/AIDS or taking immunosuppressive drugs, to reduce the risk of developing fungal lung diseases.
In IPA, the Aspergillus fungus invades the lungs and can cause inflammation, bleeding, and scarring. Symptoms include fever, cough, chest pain, and difficulty breathing. If left untreated, IPA can lead to respiratory failure and death.
IPA is diagnosed through a combination of imaging tests such as chest X-rays, CT scans, and bronchoscopy, as well as through laboratory tests that detect the presence of Aspergillus antigens or DNA in the body.
Treatment of IPA typically involves antifungal medications, such as voriconazole or caspofungin, which are given intravenously for several weeks. In severe cases, hospitalization and supportive care, such as oxygen therapy and mechanical ventilation, may be necessary.
Prevention of IPA is challenging, but efforts to reduce the risk include avoiding exposure to Aspergillus spores, managing underlying conditions that weaken the immune system, and promptly treating any respiratory infections that occur. Early detection and treatment of IPA can improve outcomes and reduce the risk of complications and death.
Aspergillus niger
Metschnikowia pulcherrima
1,2-alpha-L-fucosidase
1,4-a-glucan 6-a-glucosyltransferase
Welwitschia
Citrate dehydratase
Isocitrate dehydrogenase (NAD+)
Fumonisin B2
Gene density
Fruit rot
Ferulic acid decarboxylase
Antimicrobial properties of copper
Amine oxidase (copper-containing)
Galactosaminogalactan
Plastic degradation by marine bacteria
Aspergillus lacticoffeatus
Friedrich Siebenmann (otolaryngologist)
4-Hydroxybenzoic acid
3-hydroxybenzoate 4-monooxygenase
Aspergillus
O-pyrocatechuate decarboxylase
Mark Cushman
Fungi imperfecti
Aspergillopepsin II
Antimicrobial surface
Naturally occurring phenols
Aspergillus tubingensis
Nickel organic acid salts
Catechol 1,2-dioxygenase
Alpha-glucuronidase
Fermented tea
Sulfide
Alpha-L-arabinofuranosidase
Colonial morphology
Zinc deficiency
List of Aspergillus species
List of MeSH codes (B05)
Rhoda Williams Benham
Effects of cannabis
Aspergillus sclerotioniger
Contamination control
Kestose
Naringinase
Mold
Agave syrup
Aspergillus awamori
Indicator organism
Trehalase
Pectin lyase
Chymosin
Penicillium rubens
Vigna subterranea
Hypothiocyanite
Jennifer Loros
Black mold
Aspergillus trinidadensis
Lactase
アミログルコシダーゼ from Aspergillus niger powder, white, ~120 U/mg | Sigma-Aldrich
Quantitative iTRAQ Secretome Analysis of Aspergillus niger Reveals Novel Hydrolytic Enzymes
Molecular cloning and biochemical characterization of an α-amylase family from Aspergillus niger | Wang | Electronic Journal of...
Citric Acid Production by Wild and UV - Treated Strains of Aspergillus niger on Two Different Mineral Salt Media
|...
Aspergillus niger CBS 147353
Otitis Externa Organism-Specific Therapy: Specific Organisms and Therapeutic Regimens
Safety evaluation of the food enzyme chymosin from the genetically modified |i|Aspergillus niger|/i| strain DSM32805. | EFSA J...
Functional Stabilization of Cellulase from Aspergillus niger by Conjugation with Dextran-aldehyde | AVESİS
Citric acid production by 2-deoxyglucose-resistant mutant strains of Aspergillus niger<...
Cellobiohydrolase I enzymes (Patent) | DOE Patents
CDC - Asthma and Allergies: Prevention of Work-related Asthma: Study Search Results - NIOSH Workplace Safety and Health Topic
Healthcare Water System Repair|Natural Disasters and Severe Weather
Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses<...
Effect of agitation speed on morphological changes in Aspergillus niger hyphae in submerged fermentation system | Sudrajat |...
Secondary metabolites from a peanut-associated fungus Aspergillus niger IMBC-NMTP01 with cytotoxic, anti-inflammatory, and...
Improvement of Foreign-Protein Production in Aspergillus niger var. awamori by Constitutive Induction of the Unfolded-Protein...
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Middle Ear, Tympanic Membrane, Perforations: Practice Essentials, Epidemiology, Etiology
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Software
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Increasing Nutrients Bioavailability by Using Fibrolytic Enzymes in Dairy Buffaloes Feeding
Oryzae2
- oryzae, aspergillus niger var. (nih.gov)
- Alpha-Amylase (Aspergillus oryzae) (min. (kvsupply.com)
Flavus3
- MIXED ASPERGILLUS- aspergillus flavus var. (nih.gov)
- FAPP MIXTURE- aspergillus flavus var. (nih.gov)
- Primary cutaneous aspergillosis due to Aspergillus flavus: a case report. (medscape.com)
Terreus1
- Cutaneous infection caused by Aspergillus terreus. (medscape.com)
Fungi1
- The Minimal inhibitory concentration (MIC) value of the test substance to the fungi Aspergillus niger N402 was determined to be 25 mg/l by microplate method after 40 hr of incubation. (europa.eu)
Citric acid2
- Microbial production of citric acid by a novel Aspergillus niger EE-12 and its UV - treated strain (UV-1) were carried out in shake flask cultures using mineral salt media containing sucrose or fructose as the carbon and energy sources. (nepjol.info)
- Many mutant strains showing resistance to 2-deoxy-d-glucose (DG) on minimal medium containing glycerol as a carbon source were induced from Aspergillus niger WU-2223L, a citric acid-producing strain. (elsevierpure.com)
Lipase1
- Lipase (Aspergillus niger) (min. (kvsupply.com)
Fumigatus2
- Chloroform, ethanolic, methanolic, ethyl acetate and aqueous root extracts of Anogeissus leiocarpus and Ter- minalia avicennioides were investigated in vitro for antifungal activities against Aspergillus niger, Aspergillus fumigatus, Penicillium species, Microsporum audouinii and Trichophyton rubrum using radial growth technique. (who.int)
- Predominant fungal species were Rhodotorula-glutinis in the barking department and Aspergillus-fumigatus and Penicillium-brevicompactum on wood chip piles. (cdc.gov)
Candida albicans1
- Organism-specific therapeutic regimens for otitis externa are provided below, including those for Pseudomonas aeruginosa, Staphylococcus aureus , Candida albicans , and Aspergillus niger . (medscape.com)
Antifungal1
- Antifungal susceptibility testing of Aspergillus niger on silicon microwells by intensity-based reflectometric interference spectroscopy. (olmdiagnostics.com)
Strain1
- The food enzyme chymosin (EC 3.4.23.4) is produced with the genetically modified Aspergillus niger strain by Chr. (bvsalud.org)
Mold1
- ¹ Aspergillus is a common mold found in wet climates, on wood and is a primary decomposer of fallen leaves and vegetation. (vitacost.com)
Fermentation1
- This improved methodology has been applied to unveil the dynamic metabolite profile of one citrate over-producing A. niger isolate under citrate fermentation. (cas.cn)
Species1
- The natural lifestyle of Aspergillus niger made them more effective secretors of hydrolytic proteins and becomes critical when this species were exploited as hosts for the commercial secretion of heterologous proteins. (figshare.com)
Enzyme1
- Glade MJ, Kendra D, Kaminski MV. Improvement in protein utilization in nursing-home patients on tube feeding supplemented with an enzyme product derived from Aspergillus niger and bromelain. (gc.ca)
Gene2
- Transgenic plants of Festuca arundinacea and Lolium multiflorum were produced expressing a ferulic acid esterase (FAE) gene isolated from Aspergillus niger, following microprojectile bombardment of cell cultures. (aber.ac.uk)
- The phytase gene was obtained from Aspergillus niger var. (cbd.int)
Proteins1
- The protein secretion profile of A. niger and its mutant at different pH was explored using iTRAQ-based quantitative proteomics approach coupled with liquid chromatography-tandem mass spectrometry (LC−MS/MS). This study characterized 102 highly confident unique proteins in the secretome with zero false discovery rate based on decoy strategy. (figshare.com)
Production2
Secretory1
- Our results showed comprehensive unique secretory protein profile of A. niger , its regulation at different pH, and the potential application of iTRAQ-based quantitative proteomics for the microbial secretome analysis. (figshare.com)
Mutant1
- proteases such as tripeptidyl-peptidase, aspergillopepsin, and other enzymes including cytochrome c oxidase, cytochrome c oxidase, glucose oxidase were highly expressed in A. niger and its mutant secretion. (figshare.com)
Sample1
- a negative "no-template" control (NTC) sample consisting of elution buffer ( 2 ), and a positive control (PC) sample consisting of a mixture of 7 representative pathogens (CMV, HIV, Streptococcus agalactiae, Klebsiella pneumoniae, Cryptococcus neoformans, Aspergillus niger, and Toxoplasma gondii ) spiked at concentrations 1-2 log above the estimated limits of detection. (cdc.gov)
Found1
- 1988) studied the constituents of African medicinal avicennioides used in this study were collected in plants and found that a petroleum ether extract of Bida, Niger State, Nigeria. (who.int)
Levels1
- Highest levels of specific immunoglobulin-G were against Paecilomyces-variotii, Sporobolomyces-salmonicolor and Aspergillus- niger. (cdc.gov)