Aspergillus niger: An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.Aspergillus: A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.Aspergillus fumigatus: A species of imperfect fungi from which the antibiotic fumigatin is obtained. Its spores may cause respiratory infection in birds and mammals.Aspergillus nidulans: A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.Aspergillus flavus: A species of imperfect fungi which grows on peanuts and other plants and produces the carcinogenic substance aflatoxin. It is also used in the production of the antibiotic flavicin.Glucan 1,4-alpha-Glucosidase: An enzyme that catalyzes the hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of polysaccharide chains with the release of beta-glucose. It is also able to hydrolyze 1,6-alpha-glucosidic bonds when the next bond in sequence is 1,4.Aspergillosis: Infections with fungi of the genus ASPERGILLUS.6-Phytase: An enzyme that catalyzes the conversion of myo-inositol hexakisphosphate and water to 1L-myo-inositol 1,2,3,4,5-pentakisphosphate and orthophosphate. EC 3.1.3.26.Fungal Proteins: Proteins found in any species of fungus.Spores, Fungal: Reproductive bodies produced by fungi.Polygalacturonase: A cell wall-degrading enzyme found in microorganisms and higher plants. It catalyzes the random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. EC 3.2.1.15.Fungi: A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.Antifungal Agents: Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.Citric Acid: A key intermediate in metabolism. It is an acid compound found in citrus fruits. The salts of citric acid (citrates) can be used as anticoagulants due to their calcium chelating ability.Penicillium: A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.Genes, Fungal: The functional hereditary units of FUNGI.Glycoside HydrolasesDNA, Fungal: Deoxyribonucleic acid that makes up the genetic material of fungi.Gene Expression Regulation, Fungal: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.Xylosidases: A group of enzymes that catalyze the hydrolysis of alpha- or beta-xylosidic linkages. EC 3.2.1.8 catalyzes the endo-hydrolysis of 1,4-beta-D-xylosidic linkages; EC 3.2.1.32 catalyzes the endo-hydrolysis of 1,3-beta-D-xylosidic linkages; EC 3.2.1.37 catalyzes the exo-hydrolysis of 1,4-beta-D-linkages from the non-reducing termini of xylans; and EC 3.2.1.72 catalyzes the exo-hydrolysis of 1,3-beta-D-linkages from the non-reducing termini of xylans. Other xylosidases have been identified that catalyze the hydrolysis of alpha-xylosidic bonds.Glucose Oxidase: An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Mycobacillin: A cyclic polypeptide antibiotic isolated from culture filtrates of Bacillus subtilis that acts as an antifungal agent.Industrial Microbiology: The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.XyloseXylans: Polysaccharides consisting of xylose units.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Hyphae: Microscopic threadlike filaments in FUNGI that are filled with a layer of protoplasm. Collectively, the hyphae make up the MYCELIUM.Mycelium: The body of a fungus which is made up of HYPHAE.Aflatoxins: Furano-furano-benzopyrans that are produced by ASPERGILLUS from STERIGMATOCYSTIN. They are structurally related to COUMARINS and easily oxidized to an epoxide form to become ALKYLATING AGENTS. Members of the group include AFLATOXIN B1; aflatoxin B2, aflatoxin G1, aflatoxin G2; AFLATOXIN M1; and aflatoxin M2.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Ochratoxins: Isocoumarins found in ASPERGILLUS OCHRACEUS and other FUNGI. Ochratoxin contaminated FOOD has been responsible for cases of FOODBORNE DISEASES.Aspergillus ochraceus: An imperfect fungus that produces ochratoxins and contaminates EDIBLE GRAIN and coffee beans.Starch: Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.Endo-1,4-beta Xylanases: Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Carboxylic Ester Hydrolases: Enzymes which catalyze the hydrolysis of carboxylic acid esters with the formation of an alcohol and a carboxylic acid anion.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Cellulase: An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.Mitosporic Fungi: A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.Maltose: A dextrodisaccharide from malt and starch. It is used as a sweetening agent and fermentable intermediate in brewing. (Grant & Hackh's Chemical Dictionary, 5th ed)Food Preservatives: Substances capable of inhibiting, retarding or arresting the process of fermentation, acidification or other deterioration of foods.Pectins: High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.Pulmonary Aspergillosis: Infections of the respiratory tract with fungi of the genus ASPERGILLUS. Infections may result in allergic reaction (ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS), colonization in pulmonary cavities as fungus balls (MYCETOMA), or lead to invasion of the lung parenchyma (INVASIVE PULMONARY ASPERGILLOSIS).Rhizopus: A genus of zygomycetous fungi of the family Mucoraceae, order MUCORALES, a common saprophyte and facultative parasite of mature fruits and vegetables. It may cause cerebral mycoses in diabetes and cutaneous infection in severely burned patients.Itraconazole: A triazole antifungal agent that inhibits cytochrome P-450-dependent enzymes required for ERGOSTEROL synthesis.Aspergillosis, Allergic Bronchopulmonary: Hypersensitivity reaction (ALLERGIC REACTION) to fungus ASPERGILLUS in an individual with long-standing BRONCHIAL ASTHMA. It is characterized by pulmonary infiltrates, EOSINOPHILIA, elevated serum IMMUNOGLOBULIN E, and skin reactivity to Aspergillus antigen.beta-Glucosidase: An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.ArabinoseSterigmatocystin: A carcinogenic mycotoxin produced in high yields by strains of the common molds, Aspergillus versicolor, A. nidulans, and an unidentified species of Bipolaris. It causes necrosis of the liver and kidney and has an inhibitory effect on orotic acid incorporation into nuclear RNA.Enzyme Stability: The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.Drug Resistance, Fungal: The ability of fungi to resist or to become tolerant to chemotherapeutic agents, antifungal agents, or antibiotics. This resistance may be acquired through gene mutation.Isomaltose: A disaccharide consisting of two glucose units in an alpha (1-6) glycosidic linkage.Mycotoxins: Toxic compounds produced by FUNGI.TriazolesMycology: The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Lung Diseases, Fungal: Pulmonary diseases caused by fungal infections, usually through hematogenous spread.Polysaccharidesbeta-Mannosidase: An enzyme that catalyzes the hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. The enzyme plays a role in the lysosomal degradation of the N-glycosylprotein glycans. Defects in the lysosomal form of the enzyme in humans result in a buildup of mannoside intermediate metabolites and the disease BETA-MANNOSIDOSIS.Transformation, Genetic: Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.Mannans: Polysaccharides consisting of mannose units.Trichoderma: A mitosporic fungal genus frequently found in soil and on wood. It is sometimes used for controlling pathogenic fungi. Its teleomorph is HYPOCREA.Xylan Endo-1,3-beta-Xylosidase: A xylosidase that catalyses the random hydrolysis of 1,3-beta-D-xylosidic linkages in 1,3-beta-D-xylans.Kinetics: The rate dynamics in chemical or physical systems.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Sorbic Acid: Mold and yeast inhibitor. Used as a fungistatic agent for foods, especially cheeses.Food-Processing Industry: The productive enterprises concerned with food processing.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Aspergillus oryzae: An imperfect fungus present on most agricultural seeds and often responsible for the spoilage of seeds in bulk storage. It is also used in the production of fermented food or drink, especially in Japan.Amphotericin B: Macrolide antifungal antibiotic produced by Streptomyces nodosus obtained from soil of the Orinoco river region of Venezuela.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Invasive Pulmonary Aspergillosis: Lung infections with the invasive forms of ASPERGILLUS, usually after surgery, transplantation, prolonged NEUTROPENIA or treatment with high-doses of CORTICOSTEROIDS. Invasive pulmonary aspergillosis can progress to CHRONIC NECROTIZING PULMONARY ASPERGILLOSIS or hematogenous spread to other organs.alpha-Glucosidases: Enzymes that catalyze the exohydrolysis of 1,4-alpha-glucosidic linkages with release of alpha-glucose. Deficiency of alpha-1,4-glucosidase may cause GLYCOGEN STORAGE DISEASE TYPE II.

Purification and characterization of methylamine oxidase induced in Aspergillus niger AKU 3302. (1/718)

Crude extract of Aspergillus niger AKU 3302 mycelia incubated with methylamine showed a single amine oxidase activity band in a developed polyacrylamide gel that weakly cross-reacted with the antibody against a copper/topa quinone-containing amine oxidase (AO-II) from the same strain induced by n-butylamine. Since the organism cannot grow on methylamine and the already known quinoprotein amine oxidases of the organism cannot catalyze oxidation of methylamine, the organism was forced to produce another enzyme that could oxidize methylamine when the mycelia were incubated with methylamine. The enzyme was separated and purified from the already known two quinoprotein amine oxidases formed in the same mycelia. The purified enzyme showed a sharp symmetric sedimentation peak in analytical ultracentrifugation showing S20,w0 of 6.5s. The molecular mass of 133 kDa estimated by gel chromatography and 66.6 kDa found by SDS-PAGE confirmed the dimeric structure of the enzyme. The purified enzyme was pink in color with an absorption maximum at 494 nm. The enzyme readily oxidized methylamine, n-hexylamine, and n-butylamine, but not benzylamine, histamine, or tyramine, favorite substrates for the already known two quinoprotein amine oxidases. Inactivation by carbonyl reagents and copper chelators suggested the presence of a copper/topa quinone cofactor. Spectrophotometric titration by p-nitrophenylhydrazine showed one reactive carbonyl group per subunit and redox-cyclic quinone staining confirmed the presence of a quinone cofactor. pH-dependent shift of the absorption spectrum of the enzyme-p-nitrophenylhydrazone (469 nm at neutral to 577 nm at alkaline pH) supported the identity of the cofactor with topaquinone. Nothern blot analysis indicated that the methylamine oxidase encoding gene is largely different from the already known amine oxidase in the organism.  (+info)

Citric acid production from xylan and xylan hydrolysate by semi-solid culture of Aspergillus niger. (2/718)

Citric acid production from xylan and xylan hydrolysate was done by Aspergillus niger Yang no. 2 cultivated in a semi-solid culture using bagasse as a carrier. Yang no. 2 produced 72.4 g/l and 52.6 g/l of citric acid in 5 d from 140 g/l of xylose and arabinose, respectively. Yang no. 2 produced 51.6 g/l of citric acid in 3 d from a concentrated xylan hydrolysate prepared by cellulase treatment, containing 100 g/l of reducing sugars. Moreover, Yang no. 2 directly produced 39.6 g/l of citric acid maximally in 3 d from 140 g/l of xylan.  (+info)

Production of specific monoclonal antibodies to Aspergillus species and their use in immunohistochemical identification of aspergillosis. (3/718)

Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining.  (+info)

Kinetic characterization of Aspergillus niger N400 endopolygalacturonases I, II and C. (4/718)

Endopolygalacturonases I, II and C isolated from recombinant Aspergillus niger strains were characterized with respect to pH optimum, activity on polygalacturonic acid and mode of action and kinetics on oligogalacturonates of different chain length (n = 3-7). Apparent Vmax values using polygalacturonate as a substrate at the pH optimum, pH 4.1, were calculated as 13.8 mukat.mg-1, 36.5 mukat.mg-1 and 415 nkat.mg-1 for endopolygalacturonases I, II and C, respectively. K(m) values were < 0.15 mg.mL-1 for all three enzymes. Product progression analysis using polygalacturonate as a substrate revealed a random cleavage pattern for all three enzymes and suggested processive behavior for endopolygalacturonases I and C. This result was confirmed by analysis of the mode of action using oligogalacturonates. Processivity was observed when the degree of polymerization of the substrate exceeded 5 or 6 for endopolygalacturonase I and endopolygalacturonase C, respectively. The bond-cleavage frequencies obtained for the hydrolysis of the oligogalacturonates were used to assess subsite maps. The maps indicate that the minimum number of subsites is seven for all three enzymes. Using pectins of various degrees of esterification, it was shown that endopolygalacturonase II is the most sensitive to the presence of methyl esters. Like endopolygalacturonase II, endopolygalacturonases I, C and E, which was also included in this part of the study, preferred the non-esterified pectate. Additional differences in substrate specificity were revealed by analysis of the reaction products of hydrolysis of a mixture of pectate lyase-generated delta 4,5-unsaturated oligogalacturonates of degree of polymerization 4-8. Whereas endopolygalacturonase I showed a strong preference for generating the delta 4,5-unsaturated dimer, with endopolygalacturonase II the delta 4,5-unsaturated trimer accumulated, indicating further differences in substrate specificity. For endopolygalacturonases C and E both the delta 4,5-unsaturated dimer and trimer were observed, although in different ratios.  (+info)

Possibility for discriminating between two representative non two-state thermal unfolding models of proteins by DSC. (5/718)

Possible differences between two representative non two-state thermal unfolding mechanisms of protein are discussed concerning differential scanning calorimetry. Numerical simulations showed that, by DSC measurement, it is hard to discriminate between the independent model, which assumes independent unfolding domains in a protein, and the sequential model, which assumes intermediate(s) between native and denatured states, especially when values of molecular weight, denaturation enthalpy, and difference in denaturation temperature of each denaturation process are large. DSC curve analysis of Aspergillus niger glucoamylase based on these two models gave essentially the same thermodynamic parameters.  (+info)

Pseudoepidemic of Aspergillus niger infections traced to specimen contamination in the microbiology laboratory. (6/718)

We report a pseudo-outbreak of Aspergillus niger that followed building construction in our clinical microbiology laboratory. Because outbreaks of invasive aspergillosis have been linked to hospital construction, strategies to minimize dust in patient care areas are common practice. We illustrate that the impact of false-positive cultures on patient care should compel laboratories to prevent specimen contamination during construction.  (+info)

Oxidation of medium-chain acyl-CoA esters by extracts of Aspergillus niger: enzymology and characterization of intermediates by HPLC. (7/718)

The activities of beta-oxidation enzymes were measured in extracts of glucose- and triolein-grown cells of Aspergillus niger. Growth on triolein stimulated increased enzyme activity, especially for acyl-CoA dehydrogenase. No acyl-CoA oxidase activity was detected. HPLC analysis after incubation of triolein-grown cell extracts with decanoyl-CoA showed that beta-oxidation was limited to one cycle. Octanoyl-CoA accumulated as the decanoyl-CoA was oxidized. Beta-oxidation enzymes in isolated mitochondrial fractions were also studied. The results are discussed in the context of methyl ketone production by fungi.  (+info)

Biotransformation of (1-phenyl)ethyl hydroperoxide with Aspergillus niger: a model study on enzyme selectivity and on the induction of peroxidase activity. (8/718)

The biocatalytic enantioselective reduction of (1-phenyl)ethyl hydroperoxide (1) by the fungus Aspergillus niger to the corresponding alcohol 2 involves a multi-enzyme biotransformation of the hydroperoxide 1, as revealed by the change in the enantioselectivity as a function of incubation times. This unusual behavior is not exhibited by other fungi and seems to be restricted to A. niger. Furthermore, the peroxidase and other oxidoreductase activities of A. niger depend on the availability of metal ions such as Fe2+, Mn2+ and Zn2+ in the growth medium, since the addition of Fe2+ ions substantially (threefold) increases the enantioselectivity, whereas addition of Mn2+ and Zn2+ ions decreases it. Finally, the cold shock (4 degrees C) significantly enhances the reduction of the hydroperoxide by the microorganism A. niger.  (+info)

*Aspergillus niger

... is a fungus and one of the most common species of the genus Aspergillus. It causes a disease called black ... Abarca M, Bragulat M, Castellá G, Cabañes F (1994). "Ochratoxin A production by strains of Aspergillus niger var. niger". Appl ... Recently the strain of ATCC 16404 Aspergillus niger has been reclassified at Aspergillus brasiliensis (refer to publication by ... niger strains do produce ochratoxin A. It also produces the isoflavone orobol. A. niger is included in Aspergillus subgenus ...

*Hypothiocyanite

Aspergillus niger • Colletotrichum musae • Colletotrichum gloeosporioide • Botryodiplodia theobromae • Fusarium monoliforme • ...

*Metschnikowia pulcherrima

... and Aspergillus oryzae, but less effective on Fusarium sp. and Aspergillus niger. The UMY15 strain also proved very effective ...

*1,2-alpha-L-fucosidase

Bahl OP (1970). "Glycosidases of aspergillus niger. II. Purification and general properties of 1,2-alpha-L-fucosidase". J. Biol ...

*1,4-a-glucan 6-a-glucosyltransferase

"Studies of Aspergillus niger. Part II. Transglycosidation by Aspergillus niger". J. Chem. Lond. Soc.: 3588-3593. doi:10.1039/ ...

*Welwitschia

Seeds collected from the wild often are heavily contaminated with spores of the fungus Aspergillus niger var. phoenicis, which ... Cooper-Driver, G.A., C. Wagner and H. Kolberg (2000). Patterns of Aspergillus niger var. phoenicis (Corda) Al-Musallam ... Infection of the cones and seeds of Welwitschia mirabilis by Aspergillus niger var. phoenicis in the Namib-Naukluft Park. South ... The fungicide tebuconazole may be useful in controlling limited A. niger seed infection. The population of Welwitschia ...

*Citrate dehydratase

NEILSON NE (1955). "The aconitase of Aspergillus niger". Biochim. Biophys. Acta. 17 (1): 139-40. doi:10.1016/0006-3002(55)90330 ...

*Isocitrate dehydrogenase (NAD+)

Ramakrishnan, C.V.; Martin, S.M. (1955). "Isocitric dehydrogenase in Aspergillus niger". Arch. Biochem. Biophys. 55: 403-407. ...

*Fruit rot

Black mold caused by Aspergillus niger; Leaf spot, and others, caused by Alternaria alternata; Bitter rot caused by Glomerella ...

*Antimicrobial properties of copper

Aspergillus spp., Fusarium spp., Penicillium chrysogenum, Aspergillus niger and Candida albicans. An increased die-off of ... Aspergillus niger growth occurred on the aluminium coupons; growth was inhibited on and around copper coupons. Antimicrobial ... Aspergillus niger, Bacterium linens, Bacillus megaterium, Bacillus subtilis, Brevibacterium erythrogenes, Candida utilis, ...

*Amine oxidase (copper-containing)

Properties of amine oxidase of Aspergillus niger". Agric. Biol. Chem. 29: 864-869. doi:10.1271/bbb1961.29.864. Yamada H, Adachi ... Further properties of amine oxidase of Aspergillus niger". Agric. Biol. Chem. 29: 912-917. doi:10.1271/bbb1961.29.912. Boyer, P ... Purification and crystallisation of amine oxidase of Aspergillus niger". Agric. Biol. Chem. 29: 649-654. doi:10.1271/bbb1961.29 ...

*Galactosaminogalactan

Bardalaye, P.C., and Nordin, J.H. (1976). Galactosaminogalactan from cell walls of Aspergillus niger. J Bacteriol 125, 655-669 ... In Aspergillus fumigatus, a causative agent of aspergillosis, galactosaminogalactan is required for adherence to host tissue, ... 2013). Aspergillus Galactosaminogalactan Mediates Adherence to Host Constituents and Conceals Hyphal β-Glucan from the Immune ... Overlapping and distinct roles of Aspergillus fumigatus UDP-glucose 4-epimerases in galactose metabolism and the synthesis of ...

*4-Hydroxybenzoic acid

This enzyme can be found in Aspergillus niger. The enzyme 4-methoxybenzoate monooxygenase (O-demethylating) transforms 4- ...

*3-hydroxybenzoate 4-monooxygenase

Premkumar R, Rao PV, Sreeleela NS, Vaidyanathan CS (1969). "m-Hydroxybenzoic acid 4-hydroxylase from Aspergillus niger". Can. J ...

*O-pyrocatechuate decarboxylase

Rao PV, Moore K, Towers GH (1967). "O-pyrocatechiuc acid carboxy-lyase from Aspergillus niger". Arch. Biochem. Biophys. 122 (2 ...

*Fungi imperfecti

Aspergillus niger, has no known sexual cycle. Thus Aspergillus niger is considered a form taxon. In contrast, isolates of its ... are used to improve both the taste and the texture of cheeses Aspergillus oryzae Aspergillus sojae Aspergillus niger ... Hence the formerly classified Aspergillus species is now properly called Emericella nidulans. Phylogenetic classification of ... close relative, Aspergillus nidulans, revealed it to be the anomorphic stage of a teleomorph (the ascocarp or fruiting body of ...

*Phenols

Orobol, an isoflavone, can be isolated from Aspergillus niger. in yeasts Aromatic alcohols (example: tyrosol) are produced by ...

*Aspergillopepsin II

Aspergillus niger acid proteinase A, Aspergillus niger var. macrosporus aspartic proteinase) is a proteolytic enzyme. The ... Iio, K.; Yamasaki, M. (1976). "Specificity of acid proteinase A from Aspergillus niger var. macrosporus towards B-chain of ... Effects of acid protease-specific inhibitors on the acid proteases from Aspergillus niger var. macrosporus". J. Biochem. 80: ... Tyr26-Thr This enzyme is isolated from Aspergillus niger var. macrosporus. Chang, W.J.; Horiuchi, S.; Takahashi, K.; Yamasaki, ...

*Antimicrobial surface

Aspergillus niger and Candida albicans fungal spores. Hence, the potential to help prevent the spread of fungi that cause human ... Copper and copper alloy surfaces have demonstrated a die-off of Aspergillus spp., Fusarium spp., Penicillium chrysogenum, ...

*Catechol 1,2-dioxygenase

Ninnekar, H; Vaidyanathan, C (1981). "Catechol 1,2-dioxygenase from Aspergillus niger: Purification and properties". J. Indian ... Aspergillus niger, Brevibacterium fuscum, Acinetobacter calcoaceticus, Trichosporon cutaneum, Rhodococcus erythropolis, ...

*Alpha-glucuronidase

"Purification and characterization of intracellular alpha-glucuronidase from Aspergillus niger". Biosci. Biotechnol. Biochem. 56 ...

*Czapek medium

It was developed to grow Aspergillus niger and Penicillium camemberti. It works well for many saprophytic fungi and soil ... Dox, Arthur Wayland (1910). "The intracellular enzyms of penicillium and aspergillus: with special reference to those of ... bacteria such as species of Aspergillus, Candida, Penicillium, and Paecilomyces. Friedrich Czapek's original recipe is as ...

*Extracellular polymeric substance

and Aspergillus niger, are efficient at removing these toxic compounds. EPSs contain enzymes such as oxidoreductase and ... and Zooglea' spp.) galactosaminogalactan (Aspergillus spp.) gellan (Aureomonas elodea and Sphingomonas paucimobilis) glucuronan ...

*Fungal extracellular enzyme activity

Hanif, A (2004). "Induction, production, repression, and de-repression of exoglucanase synthesis in Aspergillus niger". ... "Biocatalytic potential of laccase-like multicopper oxidases from Aspergillus niger". Microbial Cell Factories. 11 (1): 165. doi ... Laccase activity has been documented in T. reesei, in some species in the Aspergillus genus and in freshwater ascomycetes. ...

*Dolichovespula saxonica

Fungi are also found, including Aspergillus niger and Candida krusei. Although these pathogens and fungi utilize the wasp's ... Aspergillus niger, which creates aflatoxins that can lead to respiratory disease and candidiasis; and Candida krusei, which can ...

*Fermented tea

Aspergillus niger was implicated as the main microbial organism in the Pu-erh process, but that species identification has been ... "Aspergillus luchuensis, an industrially important black Aspergillus in East Asia." PLoS ONE 8.5 (2013): e63769. Hua-Fu Wang; ... Aspergillus acidus from Puerh tea and black tea does not produce ochratoxin A and fumonisin B 2." International journal of food ... "New and revisited species in Aspergillus section Nigri." Studies in Mycology 69.1 (2011): 1-17. Haas, Doris, et al. " ...
Here, we have synthesized the silver nanoparticles (AgNPs) by using the soil fungus Aspergillus niger 2587. The results recorded from UV-vis spectrophotometer and transmission electron microscopy (TEM) support the biosynthesis and characterization of AgNPs. The synthesized silver nanoparticles have also been tested against the larvae and pupae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti. The efficacy test was performed at different concentrations for a period of different hours by the probit analysis. The larvae of Cx. quinquefasciatus have shown the 100% mortality to the synthesized AgNPs after 1 h of exposure, while the larvae of An. stephensi and Ae. aegypti were found less susceptible to the synthesized AgNPs. The pupa of Ae. aegypti has shown the efficacy LC50 4, LC90 12 and LC99 19 ppm after 2 h of exposure of the synthesized AgNPs, while, the pupae of Cx. quinquefasciatus and An. stephensi were found less susceptible to the synthesized AgNPs. By this approach, it is
The filamentous fungus Aspergillus niger was transformed with the hepatitis B virus S gene encoding the major viral envelope protein under control of the constitutive A. nidulans glyceraldehyde-3-phosphate dehydrogenase (gpdA) promoter. Approximately seven copies of the expression cassette were integrated on the genome, resulting in high-level transcription of the S gene. Production of the 24-kDa S protein and a 48-kDa S protein dimer in the membrane-associated protein fraction of the recombinant A. niger strain was shown through Western analysis. Electron microscopy of partially purified recombinant S protein revealed the formation of spherical pseudoviral particles with a diameter of 22 nm. The production level of hepatitis B pseudoviral particles was estimated to be 0.4 mg/1 culture, which compares favourably with the reported levels initially obtained in yeast, indicating the potential of the Aspergillus expression system as an alternative, cost-effective vaccine production system.. ...
Aspergillus niger ATCC ® 1015D-2™ Designation: Genomic DNA from Aspergillus niger strain 3528.7 [ATCC ® 1015™] Application:
A recombinant Aspergillus niger strain expressing the Hypocrea jecorina endoglucanase Cel7B was grown on spent hydrolysates (stillage) from sugarcane bagasse and spruce wood. The spent hydrolysates served as excellent growth media for the Cel7B-producing strain, A. niger D15[egI], which displayed higher endoglucanase activities in the spent hydrolysates than in standard medium with a comparable monosaccharide content (e.g., 2,100 nkat/ml in spent bagasse hydrolysate compared to 480 nkat/ml in standard glucose-based medium). In addition, A. niger D15[egI] was also able to consume or convert other lignocellulose-derived compounds, such as acetic acid, furan aldehydes, and phenolic compounds, which are recognized as inhibitors of yeast during ethanolic fermentation. The results indicate that enzymes can be produced from the stillage stream as a high-value coproduct in secondgeneration bioethanol plants in a way that also facilitates recirculation of process water. Copyright © 2009, American ...
Aspergillus niger ATCC ® 11414™ Designation: A-1-233 [72-4, A60, IMI 75353, NRRL 2270, VTT D-77050] Application: degrades apple pomace produces 6-phosphofructo-2-kinase produces ATP citrate lyase produces aconitic hydrase produces carnitine acetyltransferase produces citric acid citrate produces polygalacturonase endopolygalacturonase, pectinase transformation host produces citric acid from apple pomace
A rapid growing and high xylanase producing fungus Aspergillus niger SA7, was isolated from starch waste. The best yield of xylanase was 2400 U/g on the solid-state starch waste with 65-70% of initial moisture content at 28℃ for 3 days. Crude enzyme preparation contained more components of cellulase. Addition wheat bran to medium could stimulated xylanase production. 21.6% reducing sugar was obtained by hydrolysis starch waste at 45℃ culture for 24 hours.
Microorganisms generally encounter a fluctuating environment in their natural habitat and similar conditions also happen in large-scale bioreactors. In this work, the dynamic response of intracellular and extracellular metabolites of Aspergillus niger was investigated after sudden exposure to high and low excess glucose concentrations in chemostats. It was found that the steady-state pathway turnover time of the carbon flux through the central carbon metabolism (CCM) was PP pathway 50 s, EMP pathway 20 s, and TCA cycle 189 s, and an upper limit for individual metabolite concentrations in the CCM was estimated. Regardless of the glucose pulse size, little changes of amino acids levels were observed except for aspartate, which showed a significant decrease. The ATP paradox, known from other organisms, was also observed in the studied A. niger strain. However, a different response of the NAD+/NADH ratio to the glucose pulses was found in A. niger compared to previously published observations on Penicillium
Dr. Chenyu Du has more than 15 years research experience in fermentation, metabolic engineering, bioprocess development and simulation, bio-separation and biomass pretreatment. He has published 30+ high-impact publications, including 30+ papers, 2 patents and 5 book chapters. Since joined the University of Nottingham, he generated research income over £570 k as PI (including fully funded PhD studentships), £14.3 m as Co-PI and supervised, co-supervised 10 PhD students, 2 MRes students, 15+ MSc students and 15+ undergraduates. At the University of Nottingham, Chenyu has been involved in the research pertaining to the Lignocellosic Conversion to Ethanol programme (LACE programme, funded by BBSRC Sustainable Bioenergy Centre). He developed a solid state fungal fermentation based pre-treatment strategy to convert lignocellulosic raw materials into a mixture of fermentable simple sugars. In this process, cellulolytic enzymes were produced by culturing fungus (Aspergillus niger) directly on the ...
0086]1. Aarestrup F. M. 1995. Occurrence of glycopeptide resistance among Enterococcus faecium isolates from conventional and ecological poultry farms. Microb Drug Resist. 1: 255-257. [0087]2. Abarca M. L, M. R. Bragulat, G. Castella and F. J. CabanesS. 1994. Ochratoxin A Production by Strains of Aspergillus niger var. niger. Applied and environmental Microbiology. 60: 2650-2652. [0088]3. Amann, R., Ludwig, W. & Schleifer, K. H. 1988. Subunit of ATP-synthase: a useful marker for studying the phylogenetic relationship of eubacteria. J Gen Microbiol. 134: 2815-2821. [0089]4. Ananthaswamy H. N and Eisenstark A (1977) Repair of hydrogen peroxide-induced single-strand breaks in Escherichia coli deoxyribonucleic acid. Journal of Bacteriology 130(1): 187-191. [0090]5. Angeles-Lopez M., E. G. Ramos E. G. C. and C. A. Santiago (2001) Hydrogen peroxide production and resistance to nonoxinol-9 in Lactobacillus spp. isolated from vagina of reproductive age women. Revista Latinoamericana de Microbiologia ...
Isopullulanase (IPU) from Aspergillus niger A.T.C.C. (American Type Culture Collection) 9642 hydrolyses pullulan to isopanose. IPU is important for the production of isopanose and is used in the structural analysis of oligosaccharides with α-1,4 and α-1,6 glucosidic linkages. We have isolated the ipuA gene encoding IPU from the filamentous fungi A. niger A.T.C.C. 9642. The ipuA gene encodes an open reading frame of 1695 bp (564 amino acids). IPU contained a signal sequence of 19 amino acids, and the molecular mass of the mature form was calculated to be 59 kDa. IPU has no amino-acid-sequence similarity with the other pullulan-hydrolysing enzymes, which are pullulanase, neopullulanase and glucoamylase. However, IPU showed a high amino-acid-sequence similarity with dextranases from Penicillium minioluteum (61%) and Arthrobacter sp. (56%). When the ipuA gene was expressed in Aspergillus oryzae, the expressed protein (recombinant IPU) had IPU activity and was immunologically reactive with ...
The catalytic domain of glucoamylases G1 and G2 from Aspergillus niger is produced in vitro in high yield by limited proteolysis using either subtilisin Novo or subtilisin Carlsberg. Purification by affinity chromatography on an acarbose-Sepharose column followed by ion-exchange chromatography on HiLoad Q-Sepharose leads to separation of a number of structurally closely related forms of domain. The cleavage occurs primarily between Val-470 and Ala-471 as indicated by C-terminal sequencing, whereas the N-terminus is intact. Subtilisin Carlsberg, in addition, produces a type of domain which is hydrolysed before Ser-444, an O-glycosylated residue. This leaves the fragment Ser-444-Val-470 disulphide-bonded to the large N-terminal part of the catalytic domain. Subtilisin Novo, in contrast, tends to yield a minor fraction of forms extending approx. 30-40 amino-acid residues beyond Val-470. The thermostability is essentially the same for the single-chain catalytic domain and the original glucoamylases ...
Abstract: The present investigation deals with the effect of vegetative inoculum on submerged citric acid fermentation by Aspergillus niger using stirred bioreactor. All fermentations were carried out following growth on 15% raw molasses sugars for 144 h. Ferrocyanide (200 ppm) was used to control the trace metals present in the molasses medium. The maximum production of citric acid was obtained when 1.0% vegetative inoculum was used. The dry cell mass and sugar consumption were 18.5 and 96.55 g l-1, respectively. The mycelia were intermediate round pellets in their morphology. The specific productivity (qp = 0.074±0.02a g g-1 cells h-1) was several folds higher than many other workers. ...
Aspergillus niger is a versatile filamentous fungus found in the environment all over the world in soil and on decaying plant material and it has been reported to grow on a large number of foods and feeds [1]. At the same time it is a popular production host for industrial fermentations and it is used for production of both organic acids and for indigenous and heterologous enzymes and proteins [2-4]. However, A. niger produces various secondary metabolites, and among those also the important mycotoxins fumonisin B2 (FB2) and ochratoxin A (OTA) [5, 6]. Due to the ubiquity of A. niger, its production of secondary metabolites is important both from a biotechnological and a food-safety viewpoint.. Secondary metabolites are small molecules that are not directly involved in metabolism and growth. Both plants and fungi are known for producing a large number of chemically diverse secondary metabolites. While the role of some of these metabolites makes sense biologically as inferring an advantage to the ...
BioAssay record AID 568480 submitted by ChEMBL: Antifungal activity against Aspergillus niger KCTC 1231 after 2 days by twofold broth dilution method.
BioAssay record AID 1085122 submitted by ChEMBL: Antifungal activity against Aspergillus niger at 100 ug/disk after 48 hr by disk diffusion test.
Spent refinery processing catalyst is listed as a hazardous waste; the toxicity characteristic leaching procedure (TCLP) extracts of the catalyst are found to contain heavy metals at concentrations exceeding the regulated levels. In the present investigation, Aspergillus niger was adapted to single metal ions Ni, Mo or Al (at 100-2000 mg/L in steps of 100 mg/L) and then to a mixture of Ni, Mo and Al (at a mass ratio of 1:2:6, as approximately present in the spent catalyst). Adaptation experiments with single metals showed that the fungus could tolerate up to 1000 mg/L Ni, 1200 mg/L Mo and 2000 mg/L Al. In the presence of a mixture of these metals, the fungus was able to tolerate up to 100 mg/L Ni, 200 mg/L Mo and 600 mg/L Al. One-step bioleaching experiments with 1 wt% spent catalyst (of particle size <37 μm) were carried out using un-adapted and various adapted fungal strains. In contrast to the adapted strains, the un-adapted strain showed no growth in the presence of the catalyst. ...
Continuous chemostat cultures of a recombinant strain of Aspergillus niger (B1-D), engineered to produce the marker protein hen egg white lysozyme, were investigated with regard to their susceptibility to oxidative stress. The culture response to oxidative stress, produced either by addition of exogenous hydrogen peroxide (H2O2) or by high dissolved oxygen tension (DOT), was characterised in terms of the activities of two key defensive enzymes: catalase (CAT) and superoxide dismutase (SOD). Since the morphology is so critical in submerged fungal bioprocesses, the key morphological indices were analysed using a semi-automated image analysis system. Both oxidant stressors, H2O2 and elevated DOT, increased both enzyme activities, however, the extent was different: exogenous H2O2 led mainly to increased CAT activity, whereas gassing with O2 enriched air, which resulted in a DOT of 165% of air saturation, increased both enzyme activities more than 2-fold compared with the control steady state ...
An acidophilic α-amylase from Aspergillus niger RBP7 was purified after solid state fermentation on potato peel substrate. Molecular mass of the purified α-amylase was 37.5 kDa and it exhibited 1.4 mg/ml and 0.992 μ/mol/min Km and Vmax values, respectively. The enzyme was stable in the pH range from 2.0 to 6.0, at high NaCl concentration (3 M) and at temperatures between 40 °C and 70 °C. The enzyme showed an optimal activity at pH 3.0 and at 45 °C. The enzyme was inhibited by Hg2+ and was stable in the presence of different surfactants (Tween 60, Tween 80, and SDS at 1% level) and different inhibitory reagents (β-mercaptoethanol, phenylmethylsulfonyl fluoride, and sodium azide). This acidophilic amylase enzyme can digest heterogeneous food materials, i.e. the mixture of rice, fish, bread and curry with comparable activity to the commercial diastase enzymes available.. ...
Purchase high purity enzyme endo-1-5-alpha-L-Arabinanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Naringinase from Aspergillus niger was prepared and characterized to evaluate its effectiveness in debittering citrus juice. The enzyme was purified to homogeneity by sulfate fractionation and chromatographies on Q-Sepharose, Sephacryl S-200, and S-1
We have started research to analyse the molecular mechanism underlying protein secretion in A. niger. In the first instance we have isolated 9 distinct GTPase encoding genes from A. niger (sarA, sagA-H) corresponding to GTPases involved in most stages of the secretory pathway. Interestingly, several of these genes homologues are present in higher eukaryotes, but not in S. cerevisiae. These genes are used to generate a set of (conditional/deletion) mutants imposing defined blocks in the secretory pathway. To analyse transport and secretion of proteins we have developed a GFP-based secretion reporter system by fusing GFP to a carrier protein, glucoamylase (GLA). Expression of a g1aA::gfp fusion construct resulted in fluorescence of the cell wall, probably representing secreted GLA::GFP fusion protein that is retained within the extracellular matrix. Periplasmic fluorescence was only observed in young mycelium. No periplasmic fluorescence is observed in older mycelia probably due to acidification ...
ID ASPNG_1_PE330 STANDARD; PRT; 155 AA. AC ASPNG_1_PE330; A2R3F4; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Putative uncharacterized protein An14g04100;Flags: DE Precursor; (ASPNG_1.PE330). GN ORFNames=An14g04100; OS ASPERGILLUS NIGER. OC Eukaryota; Fungi; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiales; OC Trichocomaceae; mitosporic Trichocomaceae; Aspergillus. OX NCBI_TaxID=5061; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ASPNG_1.PE330. CC Aspergillus niger chromosome I CADRE full sequence 1..3379275 annotated CC Ensembl Genomes CC -!- ANNOTATIONS ORIGIN:A2R3F4_ASPNC CC -!- GENE_FAMILY: HOG000175185 [ FAMILY / ALN / TREE ] DR HOGENOM:Aspergillus_niger;CADANGAG00011346;CADANGAT00011346;CADANGAP00011126. DR EMBL; AM270320; - ; DR UniProtKB/Swiss-Prot; A2R3F4; -. DR EMBL; AM270320; CAK46646.1; -; Genomic_DNA. DR RefSeq; XP_001401034.1; ...
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Endo-1,5-alpha-L-arabinanase involved in degradation of pectin. Its preferred substrate is linear 1,5-alpha-L-arabinan (By similarity).
The [email protected] Centre provides a platform for research students to deposit their Ph.D. theses and make it available to the entire scholarly community in open access. Shodhganga Mirror Site ...
Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is one o
Common black mold found on plants. Produces abundant conidiophores. Medium: potato dextrose agar. Per plate.NOTE: Bacterial and fungal cultures will be sold and shipped to …
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Allcosmeticsource.com Gluco-amylase 100000u/g,5kg/bag,free shipping [EP170508010]- Gluco-amylase 100000u/g,5kg/bag,free shipping What is Gluco-amylase 100000u/g Gluco-amylase is derived through extraction and refinement from the Aspergillus niger, with high enzyme activity and glucose conversion. Pullulanase is derived from Bacillus subtilis, which anhydrolyze liquefied starch of alpha -D-1,6 glucoside bounds to straight chain dextrinrapidly.Cooperation with high efficiency, it can convert the starch into glucose. Application of Gluco-amylase 100000u/g This product can be
this is vandna thakur . i want to construct a growth curve for Aspergillus niger .Can u help me to give some information about this .and can u tell me that can i construct a growth curve of A.niger by taking optical density ,if yes then tell me at which wavelenth ? Actually i want to construct a standard curve for A.niger.Reply meas soon as , plz. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20110320/2c5d6ff4/attachment.html ...
Comparison of pro-inflammatory cytokines (IL-6, IL-1α and IL-1β) released by MPI and MARCO (-/-) knockout cells when stimulated by heat killed fungi- Candida albicans and Aspergillus niger
A constitutive level of a mycelium-bound lipolytic activity from Aspergillus niger MYA 135 was strongly increased by 97% in medium supplemented with 2% olive oil. The constitutive lipase showed an optimal activity in the ...
Novus Scientific (www.novusscientific.com) är en innovatör inom forskning och kommersialisering av resorberbara implantat som hjälper kroppens egen läkning. Huvudkontor, utveckling och produktion ligger i Uppsala och försäljning sker via distributörer i ett växande antal länder, för närvarande ett tjugotal.Företagets ägare och företagsledning kommer från Uppsalabaserade Radi Medical Systems AB, köpt av St Jude Medical Inc 2008. Det först utvecklade och saluförda implantatet är TIGR® Matrix (www.TIGRmatrix.com) som är ett kirurgiska nät, som bidrar med mekanisk hållfasthet tills kroppen själv återskapat vävnadens styrka för att sedan utsöndras via naturliga vägar. Främsta användningsområden är: · Att stabilisera vävnaden runt implantat i samband med bröstrekonstruktion efter cancer. · Att återställa bukväggens funktion efter komplexa ärrbråck. · Att förstärka bukförslutningen efter öppenbukskirurgi för att minska risken för sårruptur och
2.1.1 Absorption, distribution, and excretion No available data. 2.2 Toxicological studies 2.2.1 Acute studies Species Route LD50 Reference (mg/kg/b.w.) Rat oral 5000 van Eeken et al., 1986a 2.2.2 Short-term studies 2.2.2.1 Rat Groups of 9-20 male and 10-20 female Wistar rats, 89-111 g b.w., were treated by gavage for 91 days with chymosin at daily dose levels of 0, 50, 5000 or 1000 mg/kg b.w. The report did not indicate whether the commercial enzyme preparation or a more concentrated or purified form of the enzyme was used in the study. No mortality was observed in experimental animals over the course of the study. Growth, behaviour and external appearance were normal. Haematology measurements in treated animals were comparable to controls. Statistically significant changes were occasionally observed in clinical chemistry parameters, but the changes were not dose-related. Absolute and relative organ weights were unaffected by treatment, except for liver weights in females, which exhibited a ...
Abstract: The effect of solid state fermentation of Aspergillus niger on millets nutritional quality and phytic acid content were investigated in this study. Ammonium sulphate and urea as Nitrogen sources were used in combination with millet at 10 gN kg 1 substrate. The mixture was fermented for 84 h at 35 C and 90-95% RH in the laboratory. The results indicated that the crude protein content of millet was raised from 10.96-17.33%, while the crude lipid was lowered fro 3.12-2.58%. The ash content value was greatly increased from 1.36-7.94%. Five percent changes in minerals (calcium, magnesium and phosphorus) content values were also observed. Phytic acid content value of 0.4 mg 100g 1 reduced significantly to minimal 0.015 mg 100g 1. ...
A team of researchers is working to use naturally-occurring fungi for an environment-friendly recycling process to extract cobalt and lithium from waste batteries.. Get the Free Tracker App to find a SNES Classic in Stock. "The idea first came from a student who had experience extracting some metals from waste slag left over from smelting operations," said Jeffrey A. Cunningham, Ph.D. and the projects team leader.. Cunninghams team is developing the environmentally-safe way to do this with organisms found in nature -- fungi in this case -- and putting them in an environment where they can do their work.. "Fungi are a very cheap source of labor," he pointed out in his work to be presented at the 252nd National Meeting & Exposition of the American Chemical Society (ACS).. To drive the process, Cunningham and Valerie Harwood, Ph.D., both at the University of South Florida, are using three strains of fungi -- Aspergillus niger, Penicillium simplicissimum and Penicillium chrysogenum.. The team ...
Aspergillus niger endopolygalacturonase E: a cell degrading enzyme found in microorganisms and higher plants; nucleotide sequence in first source; GenBank Y14386
A gene of glucose oxidase (GOD) from Aspergillus niger Z-25 was cloned and sequenced. The entire open reading frame (ORF) consisted of 1,818 bp and encoded a putative peptide of 605 amino acids. The gene was fused to the pPICZαA plasmid and overexpressed in Pichia pastoris SMD1168. The recombinant GOD (rGOD) was secreted into the culture using MF-α factor signal peptide under the control of the AOX1 promoter. Sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated that rGOD exhibited a single band at around 94 kDa. The maximal GOD activity of approximately 40 U/mL was achieved in shake flask by induction under optimal conditions after 7 days. rGOD was purified by ammonium sulfate precipitate leading to a final specific activity of 153.46 U/mg. The optimum temperature and pH of the purified enzyme were 40 °C and 6.0, respectively. Over 88% of maximum activity was maintained below 40 °C. And the recombinant enzyme displayed a favorable stability in the pH range from 4.0 to 8.0. The ...
4665-7604 recognises glucose oxidase from Aspergillus niger.This antibody has not been cross-adsorbed and may react with homologous proteins
glucose oxidase from aspergillus niger/ACM9001370 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
The secondary substrate binding site (SBS) of Bacillus subtilis and Aspergillus niger glycoside hydrolase family 11 xylanases was studied by site-directed mutagenesis and evaluation of activity and binding properties of mutant enzymes on different substrates. Modification of the SBS resulted in an up to three-fold decrease in the relative activity of the enzymes on polymeric versus oligomeric substrates and highlighted the importance of several amino acids in the SBS forming hydrogen bonds or hydrophobic stacking interactions with substrates. Weakening of the SBS increased Kd values by up to 70-fold in binding affinity tests using natural substrates. The impact that modifications in the SBS have both on activity and on binding affinity towards polymeric substrates clearly shows that such structural elements can increase the efficiency of these single domain enzymes on their natural substrates ...
Good performance of p16/ki-67 dual-stained cytology for surveillance of women treated for high-grade CIN. Possible explanation of the basic human capacity for emotional experience Dephosphorylation of phytate by levitra vs viagra using the Aspergillus niger phytase with a high affinity for phytate. Identification of viagra substitute DNA-reactive B cells in patients with systemic lupus erythematosus. Contribution of exogenous genetic elements to the group A Streptococcus metagenome.. The FA-CS nanoparticles (NPs) were prepared by crosslinking FA-CS conjugates with sodium tripolyphosphate (STPP). The differentiation level could be sildenafil citrate estimated by two-dimensional cell morphology analysis using a conventional phase-contrast microscope. The observed preferences cannot be explained by any single structural principle, but point instead to the interaction of several principles. Catechols are co-planar cis-diols known to form female viagra pills stable, isolable complexes with borate ...
Hypersensitivity pneumonitis (HP), also referred to as extrinsic allergic alveolitis (EAA), is an inflammatory lung disease resulting from the inhalation and subsequent sensitization to a wide variety of inhaled organic dusts.1-5 HP is not mediated by IgE. It is associated with progressive pulmonary disability, irreversible lung damage, and mortality in some occupational settings.1-5 Patients often present with intermittent chills, fever, cough, and shortness of breath that begin four to eight hours after exposure to the offending dust. Aspergillus mold can be found in soil, foods, fresh water, and other natural sources including compost, barley, tobacco, and Esparto grass dust (stucco).1,3,5 These organisms grow well in decaying organic material at temperatures often attained during decomposition.5Aspergillus mold can also grow on ceiling and walls where water damage has occurred.6. No single laboratory test is diagnostic for hypersensitivity pneumonitis.1-5 Diagnosis is based on a complete ...
报告1:Moving from descriptive to predictive biology: Rational rewiring of the cell factory Aspergillus niger 报 告 人:Vera Meyer 德国柏林工业大学教授 报告题目:Moving from descriptive to predictive biology: Rational rewiring of the cell factory Aspergillus niger 报告时间:5月8日 9:30-10:30 报告地点:天津工业生物所C408会议室 报告摘要:Fungal biotechnology is currently undergoing a renaissance with important implications for its role as platform technology for the sustainable production of products, goods and drugs. Allied to this are the recent advances in fungal Systems and Synthetic Biology which can be seen as two complementary approaches to investigate the complexity of biological systems including fungi. Whereas Systems Biology analyzes cellular systems in an iterative cycle of high-throughput generation of omics data and modeling, Synthetic Biology takes a constructive approach to reengineer biological networks and to design novel ...
Books and Book Chapters. 1. Lactase production by Aspergillis Oryzae , Lambert publications, Germany, 2012. 2. Cellulase production by Aspergillus niger , Lambert publications , Germany, 2012. 3. Biological synthesis, Characterization and antimicrobial activity of silver nanoparticles from beetle leaves, In Nanoscience and drugdelivery. Apple science publication USA. Projects:. 1. Major Research project on Cellulase production by mutant fungal strain Aspergillus niger Sanctioned by University Grants Commision 2009. New Delhi, India. Abstracts. Indian Science Abstracts:. ...
Aspergillus tubingensis XghA protein: pectinolytic enzyme from Aspergillus tubingensis; amino acid sequence in first source; GenBank AJ249460
β-glucosidase production was maximum on untreated kallar grass (Leptochloa fusca) compared to other substrates (wheat bran and sigma cell-20 cellulose). But in the presence this substrate maximum amount of polysaccharides were also produced. Three types of polysaccharides (high, low and intermediate molecular weight), non covalently attached to glucosidase, were observed when Aspergillus niger NIAB 280 was grown on kallar gray wheat bran and salicin in combination. High and intermediate molecular weight polysaccharioc were alpha linked to B-glucosidase. The low molecular weight polysaccharide streak was to kallar grass and could be hemicellulose-lignin complex. Polysaccharides non-covalent attached to B-glucosidase were removed by specially designed compartmental electrophore apparatus. The enzyme was partially purified and concentrated. The non-covalently attached polysaccharides were directly associated with the B-glucosidase stability. The polysaccharide glucosidase complex was extremely ...
1CF3: 1.8 and 1.9 A resolution structures of the Penicillium amagasakiense and Aspergillus niger glucose oxidases as a basis for modelling substrate complexes.
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This enzyme blend provides a broad acting formulation derived from the plant sources Aspergillus oryzae and Aspergillus niger. It is a vegetarian alternative to Pancreatic Enzyme Formula for carbohydrate, lipid and protein digestion. These enzymes have several unique properties that enhance their ability to promote the optimal breakdown of foods and nutrients, including broad substrate specificity. This means that these enzymes have the ability to recognize and help digest a large number of target molecules. Plant enzymes are also active in a wide pH range, from about 3.5-8.5, indicating that their actions begin in the stomach and continue in the small intestine, promoting complete digestion. Pure Encapsulations Pancreatic VegEnzymes is comparable in amylase, protease and lipase profile to Pancreatic Enzyme Formula ...
Endoglucanase B (EGLB) derived from Aspergillus niger BCRC31494 has been used in the food fermentation industry because of its thermal and alkaline tolerance. It was cloned and expressed in Pichia pastoris. According to sequence analysis, the gene open reading frame comprises 1,217 bp with five introns (GenBank GQ292753). According to sequence and protein domain analyses, EGLB was assigned to glycosyl hydrolase family 5 of the cellulase superfamily. Several binding sites were found in the promoter region. The purified recombinant enzyme was induced by 0.5% methanol, and it exhibited optimal activity at 70 °C and pH 4. EGLB was stable for 3 h at temperatures below 60 °C, with more than 90% of its activity remaining. The enzyme was specific for substrates with β-1,3 and β-1,4 linkages. In Lineweaver-Burk plot analysis, the Km and Vmax values of EGLB for β-D-glucan were 134 mg/mL and 4.68 U/min/mg, respectively. The enzyme activity was increased by 1.86-fold by Co2+ and by 2-fold by Triton X-100 and
This study investigates the use of palladised Escherichia coli as a biometallic catalyst with a view to its use in deracemisation reactions. Some bacteria can reduce metals by their use as the terminal electron acceptor in the respiratory chain, and recent work using Desulfovibrio spp. has led to the generation of a palladium bionanocatalyst with superior activity to commercially available carbon-supported Pd(0) nanoparticles. This current study has investigated the use of formate as an electron donor for the reduction of soluble Pd(II) to insoluble Pd(0) nanoparticles by E. coli, and investigates the biological mechanisms responsible for the bioreduction, and for the extracellular location of the nanoparticles.Previous work has been done at the Manchester Interdisciplinary Biocentre with the directed evolution of the monoamine oxidase enzyme of Aspergillus niger (known as MAO-N), which catalyses the oxidative deamination of terminal amines. The result has been to produce an enzyme with enhanced ...
Aim: To synthesize a series of novel compounds namely, 4-(3-((substituted)-1H-pyrazol-3-yl-methylsulphonyl)-5-((3,5,6-trichloro-pyridin-2-yl-oxymethyl)-[1,2,4]triazol-4-yl-methyl)-morpholine (8a-g) and evaluate their antimicrobial activity. Material and Method: The chemical structures of newly synthesized compounds were elucidated by IR, 1H NMR, mass spectral and elemental analysis data. Their antimicrobial activities against Staphylococus aureus NCCS 2079, Bacillus Cereus, NCCS 2106, Escherichia coli NCCS 2065, Pseudomanas aeruginos NCCS 2200, Aspergillus niger ...
Commission Implementing Regulation (EU) 2017/1896 of 17 October 2017 concerning the authorisation of a preparation o endo-1,3(4)-beta-glucanase (EC 3.2.1.6) and endo-1,4-beta-xylanase (EC 3.2.1.8) produced by Aspergillus niger (NRRL 25541) as a feed additive for chickens for fattening, laying hens, pigs for fattening, minor poultry species and minor porcine species for fattening and amending Regulation (EC) No 255/2005 and repealing Regulation (EC) No 668/2003 (holder of the authorisation Andrés Pintaluba S.A ...
Biotransformation of 3-hydroxydibenzo-α-pyrone into 3,8 dihydroxydibenzo-α-pyrone and aminoacyl conjugates by Aspergillus niger isolated from native"shilajit" ...
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A Dictionary of Education (eBook) : Wallace, Susan : Education is of relevance to everyone but it involves a specialized vocabulary and terminology which may be opaque or unfamiliar to those new to the field. This UK-focused dictionary provides clear and concise definitions for over 1100 terms that anyone studying education or working in the field is likely to encounter. This second edition covers all the contemporary reforms being introduced to revise the school examinations system and to reform the process of initial teacher training in England and Wales.
Chymosin /ˈkaɪməsɪn/ or rennin /ˈrɛnɪn/ is a protease found in rennet. It is an aspartic endopeptidase belonging to MEROPS A1 family. It is produced by newborn ruminant animals in the lining of the abomasum to curdle the milk they ingest, allowing a longer residence in the bowels and better absorption. It is widely used in the production of cheese. Bovine chymosin is now produced recombinantly in E. coli, Aspergillus niger var awamori, and K. lactis as alternative resource. Chymosin is produced by ruminant animals in the lining of the abomasum. Chymosin is produced by gastric chief cells in young ruminants and some other newborn animals to curdle the milk they ingest, allowing a longer residence in the bowels and better absorption. Some other non-ruminant species, including pigs, cats, and seals, produce it. One study reported finding a chymosin-like enzyme in some human infants, but others have failed to replicate this finding. Humans have a pseudogene for chymosin that does not generate ...
Active Ingredients per 2 Soft Chews:. Yucca schidigera extract - 200 mg. Parsley leaf - 100 mg. Chamomile - 25 mg. Protease (Aspergillus oryzae) - 2044 HUT. Amylase (Aspergillus oryzae) - 546 DU. Cellulase (Aspergillus niger) - 13.6 CU. Maltase (Aspergillus niger) - 10.2 DP. Invertase (Aspergillus niger) - 0.6 INVU. Lipase (Aspergillus oryzae) - 6.8 LU. Probiotic Blend - 3.5 million CFU/serving. (Lactobacillus acidophilus, Bifidobacterium bifidum, Enterococcus thermophilus). Inactive Ingredients: Brewers Dried Yeast, Calcium Propionate, Canola Oil, Dried Cheese, Glycerin, Green Tea Extract, Liver Meal, Mixed Tocopherols, Natural Flavors, Rosemary Extract, Salmon Oil, Sorbic Acid, Soy Lecithin, Starch, Vegetable Oil, Water.. ...
Citric acid is traditionally produced by fermentation of molds since the beginning of the 20th century. However, its overall demand continually increases due to its very widespread applications ranging from traditional food use to more specific pharmaceutical and more recent biopolymer uses.. Therefore the production of citric acid is also continually improved involving the most recent tools and techniques of the field. This book discusses the metabolism of citric acid production by Aspergillus niger; biotechnological productions of citric acid; citrus fruits compounds and aging; occurrence and metabolism of citric acid by lactic acid bacteria from fruits and fermented beverages; and citric acid treatment of cancer. (Imprint: Novinka ). ...
Betaine Hydrochloride and Gentian with acid-stable digestive enzymes HCL V-Plus is designed to provide digestive support via supplemental Betaine Hydrochloride, as optimum gastric pH is required for the complete digestion of proteins and absorption of vital nutrients in the diet. Combined with an all-vegetarian herbal and enzyme blend of Gentian root, Protease 3.0 (from aspergillus niger), and Lipase (from aspergillus niger) along with Vitamin B1 as a metabolizing synergist, this enhanced pH-stable formula provides for added digestive support in the stomach. ...
Abstract. Four purified phytases isolated from Aspergillus niger and Escherichia coli were characterized biochemically and in terms of their adsorption to soils belonging to the Mollisol order. Three different organic P substrates were used to measure enzyme activity in a wide range of pH (2.3 to 9) and temperatures (−10° to 70 °C): p-nitrophenyl-phosphate, glyceraldehyde-3-phosphate and phytic acid. Phytases from A. niger showed a higher capacity to release P (36 to 50 % of P contained in the substrates, 44 to 62 μg P), than phytases from E. coli (24 to 36 %, 20 to 44 μg P). The amount of P released from organic P substrates by A. niger phytases followed the following range: p-nitrophenyl-phosphate > glyceraldehyde-3-phosphate > phytic acid whereas in E. coli phytases the order was p-nitrophenyl-phosphate/glyceraldehyde-3-phosphate > phytic acid. All phytases were active throughout the pH and temperature ranges for optimum crop production. The proportion of phytases found in the solid ...
Ascospores of the fungus Talaromyces macrosporus are sexual structures that exhibit extreme resistance to heat, high pressure, drought, and freezing (14, 15). They contain an extremely high level of trehalose (13) and have relatively low amounts of water in an aqueous environment. The spores show constitutive dormancy in rich media, and germination is triggered and synchronized by a short heat treatment at 85°C. Ascospores of T. macrosporus can germinate after 17 years of storage (40) and belong to the most resilient eucaryotic structures described hitherto. They can survive a heat treatment at 85°C for 100 min or high pressurization at 1,000 MPa for 5 min (14). Upon heat activation, the spores degrade their trehalose within 100 min, followed by a rapid release of glucose into the bathing medium up to 10% of the cell wet weight. After 2.5 h, the outer cell wall opens, and the protoplast encompassed by the inner cell wall is ejected in a fast process (seconds) termed prosilition. The ejected ...
The transfructosylating activity present in two commercial pectinase preparations (Pectinex Ultra SP-L, from Aspergillus aculeatus, and Rapidase TF, from Aspergillus niger) was studied. Pectinex Ultra SP-L, which has a high transferase/hydrolase ratio, was covalently immobilised on a polymethacrylate-based polymer (Sepabeads® EC) activated with epoxy groups. The influence of pore volume and average pore size on biocatalyst performance was studied for two of these carriers (Sepabeads EC-EP3 and EC-EP5). Several parameters that affect immobilisation such as buffer concentration, pH and amount (mg) of protein added per gram of support (varied over the range 30:1 to 200:1) were analysed. We found that Pectinex Ultra SP-L can be efficiently immobilised on these supports without adding any external salt or buffer. Using Sepabeads EC-EP5 -whose pore volume (1.67 cm3/g) and pore size (800 nm) are higher than those corresponding to Sepabeads EC-EP3- the activity towards sucrose reached 25.9 U/g ...
Candida albicans is an ubiquitous fungal commensal of human skin and mucosal surfaces, and at the same time a major life-threatening human fungal pathogen in immunocompromised individuals. Host defense mechanisms rely on the capacity of professional phagocytes to recognize Candida cell wall antigens. During the past decade, the host immune response to Candida was dissected in depth, highlighting the essential role of C-type lectin receptors, especially regarding the power of the Dectins family in discriminating between the tolerated yeast-like form of Candida and its invading counterpart, the hyphae. This review focuses on the immuno-modulatory properties of the Candida morphologies and their specific interactions with the host innate immune system in different body surfaces.
Glucoamylase adopts fine microorganisms of aspergillus niger extract from its submerged fermentation, no transglucosidase and with high percent conversion. This series pr
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Pancreatic VegEnzymes Vegetarian pancreatic enzyme alternative This enzyme blend provides a broad acting formulation derived from the plant sources Aspergillus oryzae and Aspergillus niger. It is a vegetarian alternative to Pancreatic Enzyme Formula for carbohydrate, lipid and protein digestion. These enzymes have several unique properties that enhance their ability to promote the optimal breakdown of foods and nutrients, including broad substrate specificity. This means that these enzymes have the ability to recognize and help digest a large number of target molecules. Plant enzymes are also active in a wide pH range, from about 3.5-8.5, indicating that their actions begin in the stomach and continue in the small intestine, promoting complete digestion. Pure Encapsulations Pancreatic VegEnzymes is comparable in amylase, protease and lipase profile to Pancreatic Enzyme Formula. Each 200 mg capsule of Pancreatic VegEnzymes provides the equivalent of one 500 mg capsule of Pure Encapsulations
We purified an extracellular pyranose dehydrogenase (PDH) from the basidiomycete fungus Agaricus xanthoderma using ammonium sulfate fractionation and ion-exchange and hydrophobic interaction chromatography. The native enzyme is a monomeric glycoprotein (5% carbohydrate) containing a covalently bound FAD as its prosthetic group. The PDH polypeptide consists of 575 amino acids and has a molecular mass of 65 400 Da as determined by MALDI MS. On the basis of the primary structure of the mature protein, PDH is a member of the glucose-methanol-choline oxidoreductase family. We constructed a homology model of PDH using the 3D structure of glucose oxidase from Aspergillus niger as a template. This model suggests a novel type of bi-covalent flavinylation in PDH, 9-S-cysteinyl, 8-alpha-N3-histidyl FAD. The enzyme exhibits a broad sugar substrate tolerance, oxidizing structurally different aldopyranoses including monosaccharides and oligosaccharides as well as glycosides. Its preferred electron donor ...
Effect of the Initial pH on the Performance Characteristics of the Deproteinization Process of Galactose Supplemented Shrimp Shells by Aspergillus niger in a Solid State Drum Bioreactor
Fungal strains deficient in the non-homologous end-joining (NHEJ) pathway are excellent recipient strains for gene targeting approaches. In addition, NHEJ-deficiency can facilitate the formation of heterokaryons which allows rapid identification of essential genes. However, the use of NHEJ-deficient strains can also pose some limitations for gene function analyses. For example, lack of the NHEJ pathway can interfere with phenotypic analyses and complicate complementation studies. Moreover, heterokaryons are difficult to propagate and re-transform. We describe here strategies and methods to circumvent these problems and to better exploit the power of NHEJ-deficient strains. We provide methods for the establishment of transiently deficient NHEJ strains, for improved complementation analyses using AMA1-based vectors and for fast identification and propagation of heterokaryons. The methods described are applicable for a wide range of filamentous fungi ...
Rashid, S.A.,Ibrahim D., & Aryantha, I.N.P., 2013, Effect Of Cultural Conditions On Lovastatin Production By Aspergillus Niger Sar I Using Combination Of Rice Bran And Brown Rice As Substrate, International Journal of Applied Biology and Pharmaceutical Technology, 4(2) : 150- ...
Modules of approx. 100 residues. The granular starch-binding function has been demonstrated in one case. Sometimes designated as starch-binding domains (SBD ...
This page is devoted to the particular event named above. You may use the navigation bar below to see what happend on Friday 27th of January 2012 in the other countries of the world or to visit the page where the world news related to Niger are gathered.. ...
... - Instruments Consumables Reagents Advanced BioMatrix,RANDOX,RANDOX ELISA,Biomedical, biochemical reagents, laboratory supplies, equipment, antibodies, ELISA kits, diagnostic reagents, methods of experimental techniques, general analytical instruments, material testing instruments and equipment, used laboratory equipment, instruments and equipment, life sciences, environmental monitoring equipment , measurement, measuring instruments, rotating wall bioreactor, three-dimensional tissue / stem cell culture system; microcapsule
PDIA3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 505 amino acids (25-505 a.a).
Bakgrund: Varje år får cirka 54 000 svenskar diagnosen cancer. När en person drabbas av cancer och får en palliativ diagnos inverkar det stort på de närståendes situation. Ångest och oro är många gånger större hos de närstående än hos den sjuke själv. Det är tungt att som närstående stå vid sidan om och se sin kära ha plågsamma symtom. I sjukvården hamnar närstående ofta i bakgrunden och allt fokus läggs på den sjuke. Syfte: Syftet var att belysa upplevelser av att vara närstående till en person med palliativ cancerdiagnos. Metod: Studien var en kvalitativ litteraturstudie där åtta vetenskapliga studier valdes. En innehållsanalys gjordes enligt den metod som Graneheim & Lundman beskriver. Resultat: De närstående upplevde känslomässig börda, rädsla, otrygghet, lidande, maktlöshet, stress och ångest över att stå vid sidan om en svårt sjuk familjemedlem. Pliktskyldighet och kärlek var främsta orsaker att ta på sig ansvaret för vårdandet av den sjuke, ...
CAS Number: 6363-53-7; Formula: C12H22O11 · H2O; Formula Weight: 360.31. Store at room temperature.. Find this and many more products.
LOINC Code 51529-6 Mold Allergen Mix 4 (Aspergillus fumigatus+Aspergillus niger+Aspergillus terreus+Aspergillus flavus) IgE Ab [Presence] in Serum by Multidisk
The production of ethanol was not significantly different (P,0.05) at 24 h of fermentation in substrates MUDP and MGSF (Figure 7). The same behavior was observed in studies by Turhan et al. [37], using yeast and carob extracts as nitrogen sources, with a final ethanol concentration of 45 gL-1. Substrate MMAN showed statistically significant differences (P,0.05) at 24 h of fermentation, when compared with substrates MUDP and MGSF. The use of guava seed flour as the nitrogen source yielded 43.58% more ethanol than similar studies by Peña et al. [26]. On the other hand, Tang et al. [38] reported an ethanol concentration of 67 gL-1 at 48 h of fermentation using a substrate made of molasses and ammonium sulfate. Although guava seed flour contains 2% less protein than soy hulls, it yielded ethanol concentration 2.21 times higher than that of soy hulls fermented for 9 h [39].. The ethanol yield using MMAN substrate (36 gL-1) was similar to results by Peña et al. [26] using 250 gL-1 substrate ...
Three types of chitin as α (exoskeleton of crustaceans), β (cuttlebone of cephalopods) and γ (some fungal cell wall) exist in nature. Chitosan is the most important derivative of chitin, which has various biological activities. In this study, α, β and γ-chitosans were extracted from Portunus segnis exoskeleton, Sepia pharonis ...
The objective of the study is to determine whether AN-PEP enzyme is effective in mitigating the effects of 8 g wheat protein ingestion in patients with celiac disease.. Fourteen patients with coeliac disease, 18-70 years old are recruited. During the first period, patients consume once daily a gluten-containing food product with the AN-PEP enzyme for 2 weeks. After a 2-week washout period (second period), patients enter the third period of this study, and are randomized to one of two groups and consume the same gluten-containing food product with AN-PEP or placebo.. Period 1: Patients are given a food product containing 8 g of wheat protein, to which AN-PEP is added, once daily for 14 d.. Period 2: Wash-out period of 14 d. During this period, patients will consume a gluten-free diet.. Period 3: Patients who are negative for coeliac disease symptoms during the 1st period will be randomized across two groups. Both groups receive a food product containing 8 g of wheat protein once daily for 14 d. ...
Microbial steroid biotransformation have found wide-reaching application for the production of more precious and functionalized compounds due to their high regio-and stereoselectivity. In this study, the possibility of using filamentous fungi Aspergillus brasiliensis cells in the biotransformation of progesterone (I), a C-21 steroid hormone was studied for the first time.The fungal strain was inoculated into the transformation medium which supplemented with progesterone as a substrate. Biotransformation of this steroid for 7 days afforded 3 different hydroxylated metabolites: 11α-hydroxyprogesterone (II); 14α-hydroxyprogesterone (III) and 21-hydroxyprogesterone (IV).The metabolites were separated by thin layer chromatography. Structure determinations of the metabolites were performed by comparing NMR, MS and IR spectra of starting compound with those of metabolites.These results may be of industrial importance because the metabolites can be used as precursor of some steroid drugs.
Moraes, A. M. L., M. Corrado, V. L. Holanda, G. L. Costa, M. Ziccardi, R. de Lourenço-de-Oliveira & P. C. Oliveira. Aspergillus from Brazilian mosquitoes - I. Genera Aedes and Culex from Rio de Janiero State, Mycotaxon 78: 413-422. 2001.. ABSTRACT: As part of an ongoing survey of potential biological control fungi from insects in Brazil. aspergilli were isolated from 625 mosquitoes belonging to 17 species in the genera Aedes and Culex. Two hundred and fifteen isolates belonging to 17 Aspergillus species were found. Aspergillus niger, Aspergillus flavus and Aspergillus nidulans were the most frequently isolated species. Two other species, Aspoergillus fischerianus and Aspergillus heteromorphus were new records for insects. The macro- and microcharacteristics of the Brazilian isolates are compared to those in previously published descriptions. The habitat and geographical distribution of these fungi in Brazil is reviewed.. KEYWORDS: biological control, taxonomy, entomogenous fungi. NOMENCLATURAL ...
en] The production of extracellular catalase in date flour submerged medium by a selected mutant Aspergillus phoenicis K30 (member of the Aspergillus niger group) was investigated. The strain was tested in 500 ml shake-flasks and in a 20 l bioreactor with date powder as a single carbon source. Extracellular catalase production reached 59 U ml-1 in both cases. This value is much greater than that of a wild-type strain (9.5 U ml-1). Microscopic examination showed that the extracellular catalase production was correlated with the ramified hyphals morphology in the external layer of the pellets ...
The refined crystal structures of a proteolytic fragment of glucoamylase from Aspergillus awamori var. X100 have been determined at pH 6 and 4 to a resolution of 2.2 A and 2.4 A, respectively. The models include the equivalent of residues 1 to 471 of glucoamylase from Aspergillus niger and a complete interpretation of the solvent structure. The R-factors of the pH 6 and 4 structures are 0.14 and 0.12, respectively, with root-mean-square deviations of 0.014 A and 0.012 A from expected bondlengths. The enzyme has the general shape of a doughnut. The "hole" of the doughnut consists of a barrier of hydrophobic residues at the center, which separates two water-filled voids, one of which serves as the active site. Three clusters of water molecules extend laterally from the active site. One of the lateral clusters connects the deepest recess of the active site to the surface of the enzyme. The most significant difference in the pH 4 and 6 structures is the thermal parameter of water 500, the putative ...
A total of 50 samples were examined, which consisted of 25 coins and currency notes each. Among the currency screened, 10 coins and 15 notes showed growth of single bacterial species, 11 coins and 9 notes showed the growth of two types of bacterial species each, and three coins and one note had grown three different organisms. The common fungal isolate from two of the coins was Aspergillus niger (n = 2). The bacterial isolates include Staphylococcus aureus (n = 11), Klebsiella spp. (n = 6), Escherichia coli (n = 01), Staphylococcus epidermidis (n = 3), Citrobacter spp. (n = 7), Pseudomonas spp. (n = 4), Acinetobacter spp. (n = 4), Enterobacter agglomerans (n = 2), Micrococcus spp. (n = 1), α - haemolytic Streptococcus spp. (n = 1) and Bacillus spp. (n = 35).. We have observed that 96% of the coins and 100% of the currency notes were found to be contaminated with different bacterial species, of which S. aureus, Klebsiella spp. and E. coli can be considered as pathogens, as they have been ...
During the past few years great progress has been made in the understanding of the function of starch-degrading enzymes. Combining mutational analysis, multiple sequence alignment, and three dimensional structures of enzyme substrate analogue complexes formed the basis for developing amylolytic and related enzymes through protein engineering. In the case of barley a-amylase this has involved rational site-directed mutagenesis, regional random mutagenesis and isozyme sequence exchange as well as generation of isozyme chimeras. The approach has enabled modulation of enzyme specificity and identification of the functional roles of distinct residues and regions. The mutational analysis included the complex between barley α-amylase 2 and a proteinaceous inhibitor from barley seeds, BASI (barley a-amylase/subtilisin inhibitor). The interplay between the catalytic and the starch binding domains in glucoamylase from Aspergillus niger was investigated. The two domains are connected by a highly ...
Microorganisms are the most proficient decomposers in nature, using secreted enzymes in the hydrolysis of lignocellulose. As such, they present the most abundant source for discovery of new enzymes. Acremonium alcalophilum is the only known cellulolytic fungus that thrives in alkaline conditions and can be cultured readily in the laboratory. Its optimal conditions for growth are 30°C and pH 9.0-9.2. The genome sequence of Acremonium alcalophilum has revealed a large number of genes encoding biomass-degrading enzymes. Among these enzymes, lipases are interesting because of several industrial applications including biofuels, detergent, food processing and textile industries. We identified a lipA gene in the genome sequence of Acremonium alcalophilum, encoding a protein with a predicted lipase domain with weak sequence identity to characterized enzymes. Unusually, the predicted lipase displays ≈ 30% amino acid sequence identity to both feruloyl esterase and lipase of Aspergillus niger. LipA, when
Citric acid is found naturally in citrus fruits, but producing citric acid from citrus fruits is very expensive and the demand for citric acid is greater than the available supply of citrus fruits. Therefore, when you see citric acid on a product label, you can be sure that it is a powder that was made from the fermentation of sugars. A culture of Aspergillus niger (a fungus commonly used in the pharmaceutical industry) is fed with sugar and metabolizes it into a liquid solution. The solution is mixed with lime (calcium hydroxide) which causes citrate salt to come out of the solution (precipitate). The citrate salt is then treated with sulfuric acid to make useable citric acid.. The sugars that are used for the citric acid can be derived from cane sugar, corn or wheat. In the United States, citric acid is most often derived from corn since it is a cheap, subsidized crop. In South America cane sugar is often used due to the low sugar prices, while in Europe wheat sweeteners are commonly ...
1 Adams, T. H., Wieser, J. K., & Yu, J.-H. (1998). Asexual Sporulation in Aspergillus nidulans. Microbiology and Molecular Biology Reviews, 62(2), 545-545. doi: 10.1128/mmbr.62.2.545-545.1998 https://mmbr.asm.org/content/62/1/35.full 2 David, H., Özçelik, I. Ş., Hofmann, G., & Nielsen, J. (2008). Analysis of Aspergillus nidulans metabolism at the genome-scale. BMC Genomics, 9(1), 163. doi: 10.1186/1471-2164-9-163 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386489/ 3 Galagan JE; et al. (2005). "Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae". Nature. 438 (7071): 1105-15. doi:10.1038/nature04341. PMID 16372000. https://www.ncbi.nlm.nih.gov/pubmed/16372000 4 Gugnani, H. C. (2003). Ecology and taxonomy of pathogenic aspergilli. Frontiers in Bioscience, 8(6). doi: 10.2741/1002 ...
1 Adams, T. H., Wieser, J. K., & Yu, J.-H. (1998). Asexual Sporulation in Aspergillus nidulans. Microbiology and Molecular Biology Reviews, 62(2), 545-545. doi: 10.1128/mmbr.62.2.545-545.1998 https://mmbr.asm.org/content/62/1/35.full 2 David, H., Özçelik, I. Ş., Hofmann, G., & Nielsen, J. (2008). Analysis of Aspergillus nidulans metabolism at the genome-scale. BMC Genomics, 9(1), 163. doi: 10.1186/1471-2164-9-163 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386489/ 3 Galagan JE; et al. (2005). "Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae". Nature. 438 (7071): 1105-15. doi:10.1038/nature04341. PMID 16372000. https://www.ncbi.nlm.nih.gov/pubmed/16372000 4 Gugnani, H. C. (2003). Ecology and taxonomy of pathogenic aspergilli. Frontiers in Bioscience, 8(6). doi: 10.2741/1002 ...
material, the multi-strain solid-state fermentation method was used to study the preparation technology of taurine, and the analysis of variance was employed to determine the optimal process of taurine preparation as follows: fermentation time (72 h); amount of cysteinyl acid (2.5 g), amount of methionine (2.5 g); ratio of Bacillus subtilis YS-45 to yeast S-78 to Aspergillus niger PL-39 (1:2:1). Under the optimal process, the taurine content reached 117.8 mg/g. PENG Liang; LI Bing; WANG Hui; TANG Jianzhou
Shop Feruloyl esterase ELISA Kit, Recombinant Protein and Feruloyl esterase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Suspension culture of compact callus aggregates (CCA) of Daucus carota (carrot), consisting of yellow, spherical cellular clumps displaying tissue differentiation was established. The production of peroxidase which was detected in trace amounts in dispersed carrot cell cultures was found in high amounts (5.0-14.5 U g(-1) FW) in CCA cultures. Kinetics analysis showed that CCA grew quickly for 4-12 days with a specific growth rate of 0.20 day(-1), while the peroxidase activity increased sharply after day 12. Fungal elicitors were found to effectively induce peroxidase synthesis. Peroxidase activity of 54.0 U g(-1) FW was obtained by adding Aspergillus niger elicitor at day 16 to make its concentration in medium 50 mg l(-1). (C) 1998 Elsevier Science B.V. All rights reserved.; Suspension culture of compact callus aggregates (CCA) of Daucus carota (carrot), consisting of yellow, spherical cellular clumps displaying tissue differentiation was established. The production of peroxidase which was ...
Low temperature domestic washing (i.e. < 40 °C) may be regarded as low-hygienic because thermal inactivation of microorganisms does not occur. Thus other means (i.e. bleaching agents) must be considered to compensate for low temperature and to achieve hygienic effects. In this study the biocidal activity of common bleaching systems as used in heavy duty detergents was examined using standard suspension test. The bleaching agents used for the tests were perborate, percarbonate, and phthalimodoperoxocaproic acid, as bleaching activators for low temperature bleaching tetraactetylethylenediamine (TAED) and nonanoyloxybenzenesulfonate (NOBS) were used. The bactericidal, fungicidal and virucidal properties of bleaching systems in question were determined on selected microorganisms (Enterococcus faecium, Escherichia coli, Staphylococcus aureus, Mycobacterium terrae, Candida albicans, Aspergillus niger and coliphages [phix174 and MS2]). The effects of temperature, pH and presence of soil on the ...
TY - JOUR. T1 - Mutagenesis and analysis of mold Aspergillus niger for extracellular glucose oxidase production using sugarcane molasses. AU - Singh, O. V.. PY - 2006/10. Y1 - 2006/10. N2 - Aspergillus niger ORS-4.410, a mutant of A. niger ORS-4, was generated by repeated ultraviolet (UV) irradiation. Analysis of the UV treatment dose on wild-type (WT) A. niger ORS-4, conidial survival, and frequency of mutation showed that the maximum frequency of positive mutants (25.5%) was obtained with a 57% conidial survival rate after the second stage of UV irradiation. The level of glucose oxidase (GOX) production from mutant A. niger ORS-4.410 thus obtained was 149% higher than that for WT strain A. niger ORS-4 under liquid culture conditions using hexacyanoferrate (HCF)-treated sugarcane molasses (TM) as a cheaper carbohydrate source. When subcultured monthly for 24 mo, the mutant strain had consistent levels of GOX production (2.62 ± 0.51 U/mL). Mutant A. niger ORS-4.410 was markedly different from ...
0043]In one exemplary embodiment, a transformed bacterial host cell with a mutant ferulic acid esterase gene is constructed. A mutant ferulic acid esterase gene is inserted into a pG+host5 vector. The mutant ferulic acid esterase gene is then transferred into bacterial host cells using electroporation. The mutant ferulic acid esterase then becomes part of the bacterial host cells genome by recombination. The bacterial host cells can be Lactobacillus buchneri cells. In some embodiments, the Lactobacillus buchneri cells will be Lactobacillus buchneri strain PTA-6138 cells. The mutant ferulic acid esterase gene can be a knock out. New strains of Lactobacillus buchneri with mutant, including knocked-out ferulic acid esterase, can be used as negative controls. For example, a transformed Lactobacillus buchneri strain can be used in an assay for studying the impact of ferulic acid esterase activity. One example of such an assay would be inoculating whole plant material, such as whole plant silage, with ...
Three barrows weighing 45.0 kg, fitted with simple T-cannulas in both the duodenum and terminal ileum, were assigned to diets in a 3횞3 Latin Square design experiment to determine the effects of two calcium levels (0.8% vs 0.4%) on phytase activity and nutrient balance in growing pigs. The control diet contained 0.8% calcium, with no added inorganic phosphorus (0.45% total phosphorus) and no added phytase. The two additional experimental diets contained microbial phytase (750 phytase units/kg) and supplied either 0.8% or 0.4% calcium. With added microbial phytase, ileal and total tract digestibility of total phosphorus were improved by 20.9 and 13.8 percentage units, respectively (p=0.01). The apparent duodenal and ileal digestibility of phytate phosphorus were increased by 51.8 and 49.7 percentage units. (p=0.001). Lowering dietary calcium in the presence of microbial phytase increased the digestibility of phytate phosphorus by an additional 10.9 (p=0.001) and 5.7 percentage units for duodenal ...
TY - JOUR. T1 - Species assignment and antifungal susceptibilities of black aspergilli recovered from otomycosis cases in Iran. AU - Szigeti, Gyöngyi. AU - Sedaghati, Ebrahim. AU - Mahmoudabadi, Ali Zarei. AU - Naseri, Ali. AU - Kocsubé, S.. AU - Vágvölgyi, C.. AU - Varga, J.. PY - 2012/7. Y1 - 2012/7. N2 - Black aspergilli are among the main causative agents of otomycosis worldwide. In this study, the species assignment of black aspergilli isolated from otomycosis cases in Iran was carried out using sequence analysis of part of the calmodulin gene. The results indicate that Aspergillus niger is not the only black Aspergillus species involved in otomycosis cases in Iran: Aspergillus awamori and Aspergillus tubingensis are also able to cause ear infections. Antifungal susceptibility tests were carried out against five antifungal drugs including amphotericin B, fluconazole, itraconazole, ketoconazole and terbinafine. All isolates were highly susceptible to terbinafine, while they exhibited ...
TY - JOUR. T1 - Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylation. AU - Nuetzmann, Hans-Wilhelm. AU - Reyes-Dominguez, Yazmid. AU - Scherlach, Kirstin. AU - Schroeckh, Volker. AU - Horn, Fabian. AU - Gacek, Agnieszka. AU - Schümann, Julia. AU - Hertweck, Christian. AU - Strauss, Joseph. AU - Brakhage, Axel A. PY - 2011/8/23. Y1 - 2011/8/23. N2 - Sequence analyses of fungal genomes have revealed that the potential of fungi to produce secondary metabolites is greatly underestimated. In fact, most gene clusters coding for the biosynthesis of antibiotics, toxins, or pigments are silent under standard laboratory conditions. Hence, it is one of the major challenges in microbiology to uncover the mechanisms required for pathway activation. Recently, we discovered that intimate physical interaction of the important model fungus Aspergillus nidulans with the soil-dwelling bacterium Streptomyces rapamycinicus specifically ...
Oryzin (EC 3.4.21.63, Aspergillus alkaline proteinase, aspergillopeptidase B, API 21, aspergillopepsin B, aspergillopepsin F, Aspergillus candidus alkaline proteinase, Aspergillus flavus alkaline proteinase, Aspergillus melleus semi-alkaline proteinase, Aspergillus oryzae alkaline proteinase, Aspergillus parasiticus alkaline proteinase, Aspergillus serine proteinase, Aspergillus sydowi alkaline proteinase, Aspergillus soya alkaline proteinase, Aspergillus melleus alkaline proteinase, Aspergillus sulphureus alkaline proteinase, prozyme, P 5380, kyorinase, seaprose S, semi-alkaline protease, sumizyme MP, prozyme 10, onoprose, onoprose SA, protease P, promelase) is an enzyme. This enzyme catalyses the following chemical reaction Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyse peptide amides This enzyme is a predominant extracellular alkaline endopeptidase of the mold Aspergillus oryzae. Nakagawa, Y. (1970). "Alkaline proteinases from Aspergillus". ...

Trancriptional landscape of Aspergillus niger a...Trancriptional landscape of Aspergillus niger a...

... niger conidia using both next generation RNA-sequencing and GeneChips. , Plant Genomics ... Trancriptional landscape of Aspergillus niger at breaking of conidial dormancy revealed by RNA-sequencing ... Genome-wide analysis was performed to assess the transcriptional landscape of germinating A. niger conidia using both next ... Abstract Background Genome-wide analysis was performed to assess the transcriptional landscape of germinating A. niger conidia ...
more infohttps://www.scoop.it/t/plant-genomics/p/4000606793/2013/04/26/trancriptional-landscape-of-aspergillus-niger-at-breaking-of-conidial-dormancy-revealed-by-rna-sequencing

660050: Aspergillus niger Precipitating... | LabCorp660050: Aspergillus niger Precipitating... | LabCorp

Aspergillus mold can be found in soil, foods, fresh water, and other natural sources including compost, barley, tobacco, and ... Aspergillus infection can also result in allergic bronchopulmonary aspergillosis (ABPA), a condition where airway colonization ... 5Aspergillus mold can also grow on ceiling and walls where water damage has occurred.6 ... 7 Testing for Aspergillus precipitating IgG antibodies has been shown to be of some clinical utility in the assessment of ...
more infohttps://www.labcorp.com/test-menu/20596/iaspergillus-niger-i-precipitating-antibodies-igg

Aspergillus niger Study Kit | Carolina.comAspergillus niger Study Kit | Carolina.com

Aspergillus niger Study Kit. Item # 155779 *bvseo_sdk, java_sdk, bvseo-4.0.0 ...
more infohttps://www.carolina.com/fungi/aspergillus-niger-study-kit/155779.pr

Aspergillus niger CBS 513.88 (ID 19263)  - BioProject - NCBIAspergillus niger CBS 513.88 (ID 19263) - BioProject - NCBI

Aspergillus niger CBS 513.88 Aspergillus niger CBS 513.88. Aspergillus niger CBS 513.88 RefSeq Genome ... Aspergillus; Aspergillus niger; Aspergillus niger CBS 513.88 ... Aspergillus niger CBS 513.88. Aspergillus niger CBS 513.88 ... Juhász A et al., "Comparative analysis of the complete mitochondrial genomes of Aspergillus niger mtDNA type 1a and Aspergillus ... The Aspergillus niger reference sequence (RefSeq) genome is provided by the NCBI and is available here. The protein coding ...
more infohttps://www.ncbi.nlm.nih.gov/bioproject?Cmd=Retrieve&list_uids=19263

Aspergillus niger van Tieghem ATCC ® 6277™Aspergillus niger van Tieghem ATCC ® 6277™

Aspergillus niger ATCC ® 6277™ Designation: 5373.16 Application: Resistance Testing Fungus resistance testing adhesives ... Aspergillus niger van Tieghem (ATCC® 6277™) Strain Designations: 5373.16 / Product Format: freeze-dried ... This fungus was re-identified as Aspergillus niger based on the multigene sequence analysis ...
more infohttps://www.atcc.org/en/Products/Quality_Control_Strains/By_Organization__Company/6277.aspx?slp=1

Aspergillus niger van Tieghem ATCC ® 64045™Aspergillus niger van Tieghem ATCC ® 64045™

Aspergillus niger ATCC ® 64045™ Designation: OSU A-1 Application: assay of preservatives in wood fungus resistance testing ... Aspergillus niger van Tieghem (ATCC® 64045™) Strain Designations: OSU A-1 / Product Format: freeze-dried ... Standard Test Method for Detection and Estimation of Retention of Wood Preservatives by Aspergillus Bioassaying. West ... The influence of method variables on the Aspergillus bioassay of wood preservatives. Holzforschung 40: 131-136, 1986. ...
more infohttps://www.atcc.org/Products/Quality_Control_Strains/By_Organization__Company/64045.aspx

Aspergillus niger van Tieghem ATCC ® 6275™Aspergillus niger van Tieghem ATCC ® 6275™

Aspergillus niger ATCC ® 6275™ Designation: 4247 [AM 324, CBS 131.52, CBS 769.97, DSM 1957, IFO 6341, IMI 45551, J. Friedrich ... Aspergillus niger van Tieghem (ATCC® 6275™) Strain Designations: 4247 [AM 324, CBS 131.52, CBS 769.97, DSM 1957, IFO 6341, IMI ... The use of Aspergillus niger for bioconversion of apple distillery waste. Eur. J. Appl. Microbiol. Biotechnol. 17: 243-247, ... Interesterification of butter fat by partially purified extracellular lipases from Pseudomonas putida, Aspergillus niger and ...
more infohttps://atcc.org/en/Products/Quality_Control_Strains/By_Organization__Company/6275.aspx

Infected Baerveldt Glaucoma Drainage Device by Aspergillus nigerInfected Baerveldt Glaucoma Drainage Device by Aspergillus niger

Aspergillus,/i, endophthalmitis following Baerveldt glaucoma drainage device implantation with intravitreal and topical ... Infected Baerveldt Glaucoma Drainage Device by Aspergillus niger. Nurul-Laila Salim. ,1. ,. 2 Yaakub Azhany. ,1. ,. 2 Zaidah ... The Aspergillus niger was isolated on the third day based on colony morphology and characteristics features under light ... We report a case of successfully treated infected GDD caused by Aspergillus niger with intravitreal and topical voriconazole ...
more infohttps://www.hindawi.com/journals/criopm/2015/249419/

DailyMed - Search Results for aspergillus niger var. nigerDailyMed - Search Results for aspergillus niger var. niger

MIXED ASPERGILLUS (aspergillus flavus var. oryzae, aspergillus niger var. niger, aspergillus repens, aspergillus terreus) ... FAPP MIXTURE (aspergillus flavus var. oryzae, aspergillus niger var. niger, aspergillus repens, aspergillus terreus, gibberella ... A-22 (aspergillus flavus, aspergillus fumigatus, eurotium herbariorum, aspergillus nidulans, aspergillus niger var. niger, ... oryzae, aspergillus niger var. niger, aspergillus repens, aspergillus terreus, cladosporium sphaerospermum and penicillium ...
more infohttps://dailymed.nlm.nih.gov/dailymed/search.cfm?query=aspergillus+niger+var.+niger

Lab Test: Aspergillus niger, IgE | Akron Childrens HospitalLab Test: Aspergillus niger, IgE | Akron Children's Hospital

While we are ranked among the best childrens hospitals in the country, its our compassionate approach to treatment that makes us truly exceptional. Through a combination of revolutionary treatments and extraordinary patient experiences, our care does more than heal. It brings the entire family together for emotional support and understanding across multiple locations to reach you in the community where you live. Learn more... ...
more infohttps://www.akronchildrens.org/lab_tests/Aspergillus-niger-IgE.html

Biotransformation of quinazoline and phthalazine by Aspergillus niger. | Sigma-AldrichBiotransformation of quinazoline and phthalazine by Aspergillus niger. | Sigma-Aldrich

Cultures of Aspergillus niger NRRL-599 in fluid Sabouraud medium were grown with quinazoline and phthalazine for 7 days. ... Biotransformation of quinazoline and phthalazine by Aspergillus niger.. [John B Sutherland, Thomas M Heinze, Laura K ...
more infohttps://www.sigmaaldrich.com/catalog/papers/21169055

how to do Aspergillus Niger count? - Microbiology - BioForumhow to do Aspergillus Niger count? - Microbiology - BioForum

Im facing problem in counting my Aspergillus Niger. I grew the Aspergillus Niger on Sabouraud dextrose agar with ... The problem is after 5 days incubation, my Aspergillus grows all over the aga... ... how to do Aspergillus Niger count? - posted in Microbiology: Hi, ... how to do Aspergillus Niger count?. Started by dingdong, Apr 04 ... Hi, Im facing problem in counting my Aspergillus Niger. I grew the Aspergillus Niger on Sabouraud dextrose agar with ...
more infohttp://www.protocol-online.org/forums/topic/32095-how-to-do-aspergillus-niger-count/

pdiA - Protein disulfide-isomerase precursor - Aspergillus niger - pdiA gene & proteinpdiA - Protein disulfide-isomerase precursor - Aspergillus niger - pdiA gene & protein

Aspergillus luchuensis CBS 106.47. Aspergillus brasiliensis CBS 101740. Aspergillus tubingensis CBS 134.48. Aspergillus niger ( ... Aspergillus niger. Aspergillus ochraceoroseus IBT 24754. Aspergillus luchuensis. Aspergillus kawachii (strain NBRC 4308) (White ... Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7). Aspergillus ... Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7). ...
more infohttp://www.uniprot.org/uniprot/Q12730

Aspergillus niger endopolygalacturonase E
     Summary Report | CureHunterAspergillus niger endopolygalacturonase E Summary Report | CureHunter

Aspergillus niger endopolygalacturonase E: a cell degrading enzyme found in microorganisms and higher plants; nucleotide ... Aspergillus niger endopolygalacturonase E. Subscribe to New Research on Aspergillus niger endopolygalacturonase E ... endopolygalacturonase E, Aspergillus niger; pgaE protein, Aspergillus niger. Networked: 0 relevant articles (0 outcomes, 0 ...
more infohttp://www.curehunter.com/public/keywordSummaryC110903-Aspergillus-niger-endopolygalacturonase-E.do

RCSB PDB 









- 1CZF: ENDO-POLYGALACTURONASE II FROM ASPERGILLUS NIGER Methods Report PageRCSB PDB - 1CZF: ENDO-POLYGALACTURONASE II FROM ASPERGILLUS NIGER Methods Report Page

1.68-A crystal structure of endopolygalacturonase II from Aspergillus niger and identification of active site residues by site- ...
more infohttp://www.rcsb.org/pdb/explore/materialsAndMethods.do?structureId=1CZF

Additive action of honey and starch against Candida albicans and Aspergillus niger.  - PubMed - NCBIAdditive action of honey and starch against Candida albicans and Aspergillus niger. - PubMed - NCBI

For Aspergillus niger the MIC without starch was 51% and 59%, respectively. When starch was incubated with honey and then added ... Additive action of honey and starch against Candida albicans and Aspergillus niger.. Boukraâ L1, Bouchegrane S. ... niger was 40% and 45%, with a starch concentration of 5.6% and 5.1% respectively. This study suggests that the amylase present ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/18095766

Aspergillus niger fungus, SEM - Stock Image - B250/1914 - Science Photo LibraryAspergillus niger fungus, SEM - Stock Image - B250/1914 - Science Photo Library

Aspergillus niger fungus. Coloured scanning electron micrograph (SEM) of a network (mycelium) of vegetative filaments called ... Aspergillus niger fungus. Coloured scanning electron micrograph (SEM) of a network (mycelium) of vegetative filaments called ...
more infohttps://www.sciencephoto.com/media/14735/view

Characterization and preparation of Aspergillus niger naringinase for debittering citrus juice.Characterization and preparation of Aspergillus niger naringinase for debittering citrus juice.

Naringinase from Aspergillus niger was prepared and characterized to evaluate its effectiveness in debittering citrus juice. ... Aspergillus niger / enzymology*. Beverages / analysis*. Citrus / chemistry*. Enzyme Stability. Female. Flavanones / metabolism* ... PRACTICAL APPLICATION: This study focused on characterization, preparation, and validation of naringinase from A. niger, which ...
more infohttp://www.biomedsearch.com/nih/Characterization-Preparation-Aspergillus-niger-Naringinase/22122507.html

Aspergillus niger - WikipediaAspergillus niger - Wikipedia

Aspergillus niger is a fungus and one of the most common species of the genus Aspergillus. It causes a disease called black ... Abarca M, Bragulat M, Castellá G, Cabañes F (1994). "Ochratoxin A production by strains of Aspergillus niger var. niger". Appl ... Recently the strain of ATCC 16404 Aspergillus niger has been reclassified at Aspergillus brasiliensis (refer to publication by ... niger strains do produce ochratoxin A. It also produces the isoflavone orobol. A. niger is included in Aspergillus subgenus ...
more infohttps://en.wikipedia.org/wiki/Aspergillus_niger

Structure and Function of a Pepstatin-Insensitive Acid Proteinase from Aspergillus Niger var. Macrosporus | SpringerLinkStructure and Function of a Pepstatin-Insensitive Acid Proteinase from Aspergillus Niger var. Macrosporus | SpringerLink

The fungus Aspergillus niger var. macrosporus produces two extracellular acid proteinases, proteinases A and B.1-3 The acid ... The fungus Aspergillus niger var. macrosporus produces two extracellular acid proteinases, proteinases A and B.1-3 The acid ... Circular Dichroism Aspergillus Niger Circular Dichroism Spectrum Aspartic Proteinase Acid Proteinase These keywords were added ... Part I. Isolation of two kinds of acid proteases excreted by Aspergillus niger var. macrosporus, Agr. Biol. Chem. 28: 216 (1964 ...
more infohttps://link.springer.com/chapter/10.1007/978-1-4684-6012-4_24

The black mold Aspergillus niger: flexibility as the key to success - KNAWThe black mold Aspergillus niger: flexibility as the key to success - KNAW

In an article in Nature, CBS-KNAW researchers describe fungus Aspergillus nigers flexibility to turn on the production of ... The ubiquitous fungus Aspergillus niger grows radially, forming long hyphae penetrating the substrate. It is an industrial ... Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization. In: Scientific ... A. niger is able to turn on the production of different enzyme sets in different parts of its colony. Like a Swiss army knife, ...
more infohttps://www.knaw.nl/en/news/news/the-black-mold-aspergillus-niger-flexibility-as-the-key-to-success

Possible Mosquito Control by Silver Nanoparticles Synthesized by Soil Fungus (Aspergillus niger 2587)Possible Mosquito Control by Silver Nanoparticles Synthesized by Soil Fungus (Aspergillus niger 2587)

... by using the soil fungus Aspergillus niger 2587. The results recorded from UV-vis spectrophotometer and transmission electron ... Here, we have synthesized the silver nanoparticles (AgNPs) by using the soil fungus Aspergillus niger. 2587. The results ... Soni, N. and Prakash, S. (2013) Possible Mosquito Control by Silver Nanoparticles Synthesized by Soil Fungus (Aspergillus niger ... S. Namita and P. Soam, "Aspergillus niger Metabolites Efficacies against the Mosquito Larval (Culex quinquefasciatus, Anopheles ...
more infohttp://www.scirp.org/journal/PaperInformation.aspx?PaperID=31881

Purification And Characterization Of Exo-polygalacturonase From A Newly Isolated Aspergillus Niger | 17247Purification And Characterization Of Exo-polygalacturonase From A Newly Isolated Aspergillus Niger | 17247

... produced from a newly isolated Aspergillus niger AUMC 4156 in fermentation media containing sugar beet pulp as a sole carbon s ... Exo-Polygalacturonase (Exo-PG) produced from a newly isolated Aspergillus niger AUMC 4156 in fermentation media containing ... Purification and characterization of exo-polygalacturonase from a newly isolated Aspergillus niger. ...
more infohttps://www.omicsonline.org/proceedings/purification-and-characterization-of-exopolygalacturonase-from-a-newly-isolated-aspergillus-niger-17247.html

Occupational IgE sensitisation to phytase, a phosphatase derived from Aspergillus niger. | Occupational & Environmental MedicineOccupational IgE sensitisation to phytase, a phosphatase derived from Aspergillus niger. | Occupational & Environmental Medicine

Aspergillus niger. The amount of IgE binding phytase in Aspergillus niger was estimated to be between 0.1% and 1% of the ... OBJECTIVE: Phytase is a phosphatase derived from Aspergillus niger that enhances phosphate bioavailability in the gut, and ...
more infohttp://oem.bmj.com/content/56/7/454
  • A. niger is also cultured for the extraction of the enzyme, glucose oxidase, used in the design of glucose biosensors, due to its high affinity for β-D-glucose. (wikipedia.org)
  • A. niger is able to turn on the production of different enzyme sets in different parts of its colony. (knaw.nl)
  • Aspergillus niger CFR 335 and A. ficuum produced a maximum of 60.6 U/gds and 38 U/gds of the enzyme, respectively, in wheat bran solid substrate medium. (hindawi.com)
  • A maximum of 9.6 and 8.2 U/mL of enzyme activity was observed in SmF by A. niger CFR 335 and A.ficuum , respectively, when grown in potato dextrose broth. (hindawi.com)
  • In the present study the investigators wish to determine if co-administration of such an enzyme, a prolyl endoprotease derived from the food grade organism Aspergillis niger (AN-PEP), is capable of detoxifying 8 grams of gluten in a commercial food product. (clinicaltrials.gov)
  • Aspergillus fumigatus is the main causative agent of aspergillosis. (springer.com)
  • Our previous studies showed that A. fumigatus and A. niger have different behavior in the presence of type II alveolar A549 epithelial cells. (springer.com)
  • A. fumigatus conidia are more efficiently internalized by these cells and germination is delayed when compared to A. niger . (springer.com)
  • Here it is shown that global gene expression of A. fumigatus and A. niger is markedly different upon contact with A549 cells. (springer.com)
  • A total of 545 and 473 genes of A. fumigatus and A. niger , respectively, were differentially expressed when compared to growth in the absence of A549 cells. (springer.com)
  • The orthologues of A. fumigatus genes involved in hypoxia regulation and heat shock were also up-regulated in A. niger, whereas thioredoxin reductase and allergen genes were found up-regulated in A. fumigatus but down-regulated in A. niger . (springer.com)
  • Our dual transcriptomic analysis supports earlier observations of a marked difference in life style between A. fumigatus and A. niger when grown in the presence of type II epithelial cells. (springer.com)
  • For example, A. niger glucoamylase is used in the production of high-fructose corn syrup, and pectinases are used in cider and wine clarification. (wikipedia.org)
  • acetone, chloroform, distilled water, ethanol and methanol extractions of two weeds namely, Parthenium hysterophorus and Datura stramonium were evaluated in vitro against Sclerotium rolfsii and Aspergillus niger , the causal agents of collar rot and seed rot of chickpea, respectively. (cabi.org)
  • The catalytic domain of glucoamylases G1 and G2 from Aspergillus niger is produced in vitro in high yield by limited proteolysis using either subtilisin Novo or subtilisin Carlsberg. (biochemj.org)
  • Aspergillus infection can also result in allergic bronchopulmonary aspergillosis (ABPA), a condition where airway colonization of individuals with asthma or cystic fibrosis results in increased inflammation and destruction of bronchial structural elements. (labcorp.com)
  • Infection of onion seedlings by A. niger can become systemic, manifesting only when conditions are conducive. (wikipedia.org)
  • In the first instance we have isolated 9 distinct GTPase encoding genes from A. niger (sarA, sagA-H) corresponding to GTPases involved in most stages of the secretory pathway. (aspergillus.org.uk)
  • Aspergillosis is, in particular, frequent among horticultural workers who inhale peat dust, which can be rich in Aspergillus spores. (wikipedia.org)
  • At present there is lesser experience with intravitreal injection of voriconazole as compared to Amphotericin B. We present a case of successfully treated Aspergillus endophthalmitis following Baerveldt glaucoma drainage device implantation with intravitreal and topical voriconazole. (hindawi.com)
  • We report a case of successfully treated infected GDD caused by Aspergillus niger with intravitreal and topical voriconazole along with removal of GDD. (hindawi.com)
  • Another use for A. niger within the biotechnology industry is in the production of magnetic isotope-containing variants of biological macromolecules for NMR analysis. (wikipedia.org)
  • A. niger is cultured for the industrial production of many substances. (wikipedia.org)
  • Ochratoxin A production by strains of Aspergillus niger var. (wikipedia.org)
  • In this study, a comparison was made between submerged (SmF) and solid-state fermentations (SSF) for the production of phytase from Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01. (hindawi.com)
  • Among the inorganic and organic nitrogen sources, ammonium nitrate in concentration of 0.5% was found to be a favorable nitrogen source for phytase production in Aspergillus niger. (scirp.org)
  • Maximal phytase activity of Aspergillus niger was detected in media with 1.0% sucrose as a carbon source. (scirp.org)
  • Standard Test Method for Detection and Estimation of Retention of Wood Preservatives by Aspergillus Bioassaying. (atcc.org)
  • S. Namita and P. Soam, "Aspergillus niger Metabolites Efficacies against the Mosquito Larval (Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti) Population after Column Chromatography," American Journal of Microbiology, Vol. 2, No. 1, 2011, pp. 15-20. (scirp.org)
  • citation needed] Recent evidence suggests some true A. niger strains do produce ochratoxin A. It also produces the isoflavone orobol. (wikipedia.org)
  • Search Results for aspergillus niger var. (nih.gov)
  • Among all the solvent extracts, methanol extract of P. hysterophorus had potential antifungal activity against S. rolfsii and A. niger . (cabi.org)
  • The influence of method variables on the Aspergillus bioassay of wood preservatives. (atcc.org)
  • Aspergillus niger isolate was cultivated for 72 h at 30˚C on cultivation medium. (scirp.org)