Aspergillus nidulans
Aspergillus
Aspergillus fumigatus
Aspergillus flavus
Sterigmatocystin
Gene Expression Regulation, Fungal
Hyphae
Benomyl
Reproduction, Asexual
Molecular Sequence Data
Amino Acid Sequence
Mutation
Aflatoxins
Transformation, Genetic
Cloning, Molecular
Penicillium
Antifungal Agents
Base Sequence
Sequence Homology, Amino Acid
Gene Deletion
Aspergillus oryzae
Aspergillus ochraceus
Genetic Complementation Test
Fungi
Mitosis
Diploidy
Acetate-CoA Ligase
RNA, Fungal
Phenotype
Homogentisate 1,2-Dioxygenase
Cell Wall
Suppression, Genetic
Multigene Family
Haploidy
Sequence Alignment
Pulmonary Aspergillosis
Hydro-Lyases
Drug Resistance, Fungal
Penicillins
Enzyme Repression
Gliotoxin
Aspergillosis, Allergic Bronchopulmonary
Genes, Regulator
Chromosomes, Fungal
Culture Media
Oxidoreductases
Expression of atrC - encoding a novel member of the ATP binding cassette transporter family in Aspergillus nidulans - is sensitive to cycloheximide. (1/1128)
A new member of the ABC superfamily of transmembrane proteins in Aspergillus nidulans has been cloned and characterized. The topology of conserved motifs subgroups AtrC in the P-glycoprotein cluster of ABC permeases, the members of this subfamily, are known to participate in multidrug resistance (MDR) in diverse organisms. Alignment results display significant amino acid similarity to AfuMDR1 and AflMDR1 from Aspergillus fumigatus and flavus, respectively. Northern analysis reveals that atrC mRNA levels are 10-fold increased in response to cycloheximide. Evidence for the existence of eight additional hitherto unpublished ABC transporter proteins in A. nidulans is provided. (+info)Insertion analysis of putative functional elements in the promoter region of the Aspergillus oryzae Taka-amylase A gene (amyB) using a heterologous Aspergillus nidulans amdS-lacZ fusion gene system. (2/1128)
Expression of the Taka-amylase A gene (amyB) of Aspergillus oryzae is induced by starch or maltose. The A. oryzae amyB gene promoter contains three highly conserved sequences, designated Regions I, II, and III, compared with promoter regions of the A. oryzae glaA encoding glucoamylase and the agdA encoding alpha-glucosidase. To identify the function of these sequences within the amyB promoter, various fragments containing conserved sequences in the amyB promoter were introduced into the upstream region of the heterologous A. nidulans amdS gene (encoding acetamidase) fused to the Escherichia coli lacZ gene as a reporter. Introduction of the sequence between -290 to -233 (the number indicates the distance in base pairs from the translation initiation point (+1)) containing Region III significantly increased the expression of the lacZ reporter gene in the presence of maltose. The sequence between -377 to -290 containing Region I also increased the lacZ activity, but its maltose inducibility was less than that of Region III. The sequence between -233 to -181 containing Region II had no effect on the expression. These results indicated that Region III is most likely involved in the maltose induction of the amyB gene expression. (+info)The GATA factor AreA is essential for chromatin remodelling in a eukaryotic bidirectional promoter. (3/1128)
The linked niiA and niaD genes of Aspergillus nidulans are transcribed divergently. The expression of these genes is subject to a dual control system. They are induced by nitrate and repressed by ammonium. AreA mediates derepression in the absence of ammonium and NirA supposedly mediates nitrate induction. Out of 10 GATA sites, a central cluster (sites 5-8) is responsible for approximately 80% of the transcriptional activity of the promoter on both genes. We show occupancy in vivo of site 5 by the AreA protein, even under conditions of repression. Sites 5-8 are situated in a pre-set nucleosome-free region. Under conditions of expression, a drastic nucleosomal rearrangement takes place and the positioning of at least five nucleosomes flanking the central region is lost. Remodelling is strictly dependent on the presence of an active areA gene product, and independent from the NirA-specific and essential transcription factor. Thus, nucleosome remodelling is independent from the transcriptional activation of the niiA-niaD promoter. The results presented cast doubts on the role of NirA as the unique transducer of the nitrate induction signal. We demonstrate, for the first time in vivo, that a GATA factor is involved directly in chromatin remodelling. (+info)Unique DNA binding specificity of the binuclear zinc AlcR activator of the ethanol utilization pathway in Aspergillus nidulans. (4/1128)
AlcR is the transcriptional activator in Aspergillus nidulans, necessary for the induction of the alc gene cluster. It belongs to the Zn2Cys6 zinc cluster protein family, but contains some striking differences compared with other proteins of this group. In this report, we show that no dimerization element is present in the entire AlcR protein which occurs in solution as a monomer and binds also to its cognate sites as a monomer. Another important feature of AlcR is its unique specificity for single sites occurring naturally as inverted or direct repeats and sharing a common motif, 5'-(T/A)GCGG-3'. Like most other Zn2Cys6 proteins, AlcR contacts directly with the CGG triplet and, in addition, the upstream adjacent guanine is required for high affinity binding. We also establish that the flanking regions outside the core play an essential role in tight binding. From our in vitro analysis, we propose an optimal AlcR-binding site which is 5'-PuNGCGG-AT rich 3'. (+info)nimO, an Aspergillus gene related to budding yeast Dbf4, is required for DNA synthesis and mitotic checkpoint control. (5/1128)
The nimO predicted protein of Aspergillus nidulans is related structurally and functionally to Dbf4p, the regulatory subunit of Cdc7p kinase in budding yeast. nimOp and Dbf4p are most similar in their C-termini, which contain a PEST motif and a novel, short-looped Cys2-His2 zinc finger-like motif. DNA labelling and reciprocal shift assays using ts-lethal nimO18 mutants showed that nimO is required for initiation of DNA synthesis and for efficient progression through S phase. nimO18 mutants abrogated a cell cycle checkpoint linking S and M phases by segregating their unreplicated chromatin. This checkpoint defect did not interfere with other checkpoints monitoring spindle assembly and DNA damage (dimer lesions), but did prevent activation of a DNA replication checkpoint. The division of unreplicated chromatin was accelerated in cells lacking a component of the anaphase-promoting complex (bimEAPC1), consistent with the involvement of nimO and APC/C in separate checkpoint pathways. A nimO deletion conferred DNA synthesis and checkpoint defects similar to nimO18. Inducible nimO alleles lacking as many as 244 C-terminal amino acids supported hyphal growth, but not asexual development, when overexpressed in a ts-lethal nimO18 strain. However, the truncated alleles could not rescue a nimO deletion, indicating that the C terminus is essential and suggesting some type of interaction among nimO polypeptides. (+info)The abfB gene encoding the major alpha-L-arabinofuranosidase of Aspergillus nidulans: nucleotide sequence, regulation and construction of a disrupted strain. (6/1128)
Using a DNA fragment containing the Aspergillus niger abfB gene as a probe, the homologous Aspergillus nidulans gene, designated abfB, has been cloned from a genomic library containing size-selected HindIII fragments. The nucleotide sequence of the A. nidulans abfB gene shows strong homology with the A. niger abfB, Trichoderma reesei abf-1 and Trichoderma koningii alpha-L-arabinofuranosidase/beta-xylosidase genes. Regulation of abfB expression has been investigated in cultures induced with L-arabitol. The accumulation of abfB mRNA, total alpha-L-arabinofuranosidase activity and AbfB protein levels have been determined in a wild-type A. nidulans strain as well as in different mutant strains. These strains are affected either in their response to ambient pH (paIA1 and pacC(c)14 mutants), carbon catabolite repression (creA(d)4 mutant), the ability to utilize L-arabitol as a carbon source (araA1 mutant) or a combination of both latter genotypes (araA1 creA(d)4). The results obtained indicate that the expression of the A. nidulans abfB gene was higher at acidic pHs and was superinduced in this double mutant. Furthermore, disruption of the abfB gene demonstrated that in A. nidulans AbfB is the major p-nitrophenyl alpha-L-arabinofuranoside-hydrolysing activity but at least one minor activity is expressed, which is involved in the release of L-arabinose from polysaccharides. (+info)Zinc-regulated biosynthesis of immunodominant antigens from Aspergillus spp. (7/1128)
ASPND1 and ASPF2 are immunodominant antigens from Aspergillus nidulans and A. fumigatus, respectively, that are readily synthesized in infections in the human host, as demonstrated by their reactivity with more than 80% of sera from patients with aspergilloma or allergic bronchopulmonary aspergillosis. We demonstrate here that both antigens are exclusively produced under situations of low bioavailability of free Zn2+. Addition of micromolar concentrations of Zn2+ to the culture medium strongly stimulated Aspergillus growth but totally inhibited ASPND1 or ASPF2 production. This effect was specific, since other divalent metals had no effect. Removal of endogenous Zn2+ by a chelator also stimulated ASPND1 production, and the effect was specifically reversed by Zn2+. These results suggest a possible role of these antigens in the survival of the fungus in the lungs. (+info)Disruption of phacA, an Aspergillus nidulans gene encoding a novel cytochrome P450 monooxygenase catalyzing phenylacetate 2-hydroxylation, results in penicillin overproduction. (8/1128)
Aspergillus nidulans utilizes phenylacetate as a carbon source via homogentisate, which is degraded to fumarate and acetoacetate. Mutational evidence strongly suggested that phenylacetate is converted to homogentisate through two sequential hydroxylating reactions in positions 2 and 5 of the aromatic ring. Using cDNA substraction techniques, we have characterized a gene, denoted phacA, whose transcription is strongly induced by phenylacetate and which putatively encodes a cytochrome P450 protein. A disrupted phacA strain does not grow on phenylacetate but grows on 2-hydroxy- or 2, 5-dihydroxyphenylacetate. Microsomal extracts of the disrupted strain are deficient in the NADPH-dependent conversion of phenylacetate to 2-hydroxyphenylacetate. We conclude that PhacA catalyzes the ortho-hydroxylation of phenylacetate, the first step of A. nidulans phenylacetate catabolism. The involvement of a P450 enzyme in the ortho-hydroxylation of a monoaromatic compound has no precedent. In addition, PhacA shows substantial sequence divergence with known cytochromes P450 and defines a new family of these enzymes, suggesting that saprophytic fungi may represent a source of novel cytochromes P450. Phenylacetate is a precursor for benzylpenicillin production. phacA disruption increases penicillin production 3-5-fold, indicating that catabolism competes with antibiotic biosynthesis for phenylacetate and strongly suggesting strategies for Penicillium chrysogenum strain improvement by reverse genetics. (+info)The symptoms of aspergillosis depend on the location and severity of the infection. In the lungs, it may cause coughing, fever, chest pain, and difficulty breathing. In the sinuses, it can cause headaches, facial pain, and nasal congestion. In the brain, it can cause seizures, confusion, and weakness.
Aspergillosis is typically diagnosed through a combination of imaging tests such as chest X-rays, CT scans, and MRI scans, along with a biopsy to confirm the presence of Aspergillus fungi.
Treatment of aspergillosis depends on the severity and location of the infection. In mild cases, treatment may involve antifungal medications and supportive care such as oxygen therapy and pain management. In severe cases, treatment may require hospitalization and intravenous antifungal medications.
Preventive measures for aspergillosis include avoiding exposure to dusty or damp environments, managing chronic conditions such as asthma and COPD, and taking antifungal medications as prescribed.
Aspergillosis can be a serious condition, especially in people with weakened immune systems, such as those with cancer, HIV/AIDS, or taking immunosuppressive drugs. In severe cases, aspergillosis can lead to life-threatening complications such as respiratory failure, sepsis, and organ damage.
In conclusion, aspergillosis is a common fungal infection that can affect various parts of the body, and it can be serious and potentially life-threatening, especially in people with weakened immune systems. Early diagnosis and appropriate treatment are essential to prevent complications and improve outcomes.
Pulmonary aspergillosis is a type of fungal infection that affects the lungs and is caused by the fungus Aspergillus. It can occur in people with weakened immune systems, such as those with cancer, HIV/AIDS, or taking immunosuppressive drugs following an organ transplant.
The symptoms of pulmonary aspergillosis can vary depending on the severity of the infection and may include:
* Coughing up blood or mucus
* Chest pain or tightness
* Fever
* Shortness of breath
* Chills
* Weight loss
In severe cases, pulmonary aspergillosis can lead to respiratory failure, which can be life-threatening.
Pulmonary aspergillosis is diagnosed through a combination of imaging tests such as chest X-rays, CT scans, and fungal cultures. Treatment typically involves antifungal medications and supportive care to manage symptoms and prevent complications. In severe cases, hospitalization may be necessary to provide oxygen therapy and other respiratory support.
Prevention is key in avoiding pulmonary aspergillosis, especially for individuals with weakened immune systems. This includes avoiding exposure to fungal spores, managing underlying health conditions, and taking antifungal medications as prescribed. Early diagnosis and treatment can significantly improve outcomes for those affected by this condition.
The main cause of ABPA is exposure to airborne spores of the fungus Aspergillus, which are commonly found in soil and decaying organic matter. Individuals with a pre-existing allergic condition may be more susceptible to developing an allergic reaction to these spores, leading to inflammation and damage to the airways.
Diagnosis of ABPA typically involves a combination of physical examination, medical history, and diagnostic tests such as chest X-rays, CT scans, and bronchoscopy with biopsy. Treatment for ABPA typically involves corticosteroids to reduce inflammation and antifungal medications to treat any underlying infection. In severe cases, hospitalization may be necessary to provide oxygen therapy and other supportive care.
Prevention of ABPA includes avoiding exposure to known allergens and maintaining good respiratory hygiene. This can involve regularly cleaning and disinfecting surfaces and objects, using HEPA filters in air purifiers, and wearing a mask when working with or around potentially contaminated materials.
Prognosis for ABPA is generally good if treated promptly and effectively, but untreated cases can lead to serious complications such as respiratory failure and other organ damage. With proper management and prevention strategies in place, individuals with ABPA can lead active and fulfilling lives.
Aspergillus nidulans
Aspergillus rugulosus
Mycoremediation
Aspergillus quadrilineatus
Gene density
Hülle cell
List of model organisms
Velvet complex
Plant-fungus horizontal gene transfer
Nucleobase cation symporter-1
Aspergillus terreus
Obaid Siddiqi
Fungus
Aspergillus spectabilis
Aspergillus bicolor
Balanced lethal systems
Aspergillus navahoensis
A. Jamie Cuticchia
Propionyl-CoA
Nuclear pore
Echinocandin B
Sterigmatocystin
Aspergillus
Dihydromaltophilin
Point mutation
Georges Delacroix
TEAD2
Fungi imperfecti
Fellutamide
Aspergillus fumigatus
Cell cycle
List of Aspergillus species
List of MeSH codes (B05)
BZIP intron RNA motif
Linoleate 8R-lipoxygenase
PRPF4B
MAP4K5
NEK3
STK3
Castor oil
TLK1
NIMA-related kinase 1
FAM214A
MAP3K13
L-ornithine N5 monooxygenase
DYRK1A
NEK8
Dynactin
MARK2
FR901483
Homothallism
Interspecies quorum sensing
Triton X-100
Sulfite reductase
Anidulafungin
Gene: ANIA 00859 - Summary - Aspergillus nidulans - Ensembl Genomes 56
Cloning, characterization of the acyl-CoA : 6-amino penicillanic acid acyltransferase gene of Aspergillus nidulans and linkage...
Hyperbaric oxygen therapy (HBOT) | Aspergillus nidulans | India
DailyMed - MUCOR PLUMBEUS liquid
OAK, CALIFORNIA MIX- quercus agrifolia/quercus kelloggii liquid
PINE MIX- pinus echinata/pinus...
Role of β-galactofuranose and β-glucan in Aspergillus nidulans hyphal cell wall ultrastructure and physical properties
Constant Systems | Pichia pastoris - Aspergillus nidulans - Membrane proteins - UK Canada - 2011
RTECS:JM5690000 - Dipyrido(1,2-a;2',1'-c)pyrazinediium, 6,7-dihydro-, dibromide - The Registry of Toxic Effects of Chemical...
Natural Care bioAllers Mold, Yeast & Dust -- 1 fl oz - Vitacost
Environmental Hotspots for Azole Resistance Selection of Aspergillus fumigatus, the Netherlands - Volume 25, Number 7-July 2019...
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Databases/Retrieval Systems/Datasets on the Internet - Citing Medicine - NCBI Bookshelf
Nutrients | Free Full-Text | Towards an Integrative Understanding of tRNA Aminoacylation-Diet-Host-Gut Microbiome Interactions...
Biomarkers Search
Identification of natural CTXM-15 inhibitors from aqueous extract of endophytic bacteria Cronobactersakazaki - PubMed
Differential bacterial capture and transport preferences facilitate co-growth on dietary xylan in the human gut | Nature...
DailyMed - TRIAMTERENE AND HYDROCHLOROTHIAZIDE capsule
Kristen M. Horstmann Week 2 Journal - OpenWetWare
Sanjay Kulkarni, MD, MHCM, FACS | Yale School of Medicine
Microbial L-asparaginase as a Potential Therapeutic Agent for the Treatment of Acute Lymphoblastic Leukemia: The Pros and Cons
Eukaryotic Cell | Scholars@Duke
MeSH Browser
Details - Public Health Image Library(PHIL)
TREE NUMBER DESCRIPTOR
Project : USDA ARS
Fumigatus15
- Azole resistance is a major concern for treatment of infections with Aspergillus fumigatus . (cdc.gov)
- Stockpiles of plant waste contained the highest levels of azole-resistant A. fumigatus , and active aerobic composting reduced Aspergillus colony counts. (cdc.gov)
- Aspergillus fumigatus is a saprophytic mold whose natural habitat is decaying plant material ( 1 ). (cdc.gov)
- Calcineurin controls growth, morphology, and pathogenicity in Aspergillus fumigatus. (duke.edu)
- Disruption of a nonribosomal peptide synthetase in Aspergillus fumigatus eliminates gliotoxin production. (duke.edu)
- Low genetic variation and no detectable population structure in aspergillus fumigatus compared to closely related Neosartorya species. (duke.edu)
- 2019. Efficacy of Olorofim (F901318) against Aspergillus fumigatus, A. nidulans, and A. tanneri in Murine Models of Profound Neutropenia and Chronic Granulomatous Disease. (nih.gov)
- The type of mold that causes most forms of aspergillosis, Aspergillus fumigatus, is common in our environment. (web.app)
- Le aspergillosi più comuni sono classificate in: El hongo Aspergillus fumigatus es un patógeno oportunista. (web.app)
- M 2021-03-30 · Aspergillus fumigatus Fresenius, 1863. (web.app)
- homotypic synonym: Aspergillus fumigatus Fresen. (web.app)
- Det finns generellt 5 former av aspergillosEtiologiAspergillus fumigatus (vanligast), Aspergillus flavus (särskilt vid nedsatt immunförsvar 2007-11-01 17 hours ago 1999-04-01 Aspergillus fumigatus is the most common cause of human Aspergillus infections (both azole-resistant and nonresistant infections). (web.app)
- A. fumigatus, A. terreus, A. nidulans, A. flavus, and A. niger were inoculated on Czapek-Dox agar and grown Aspergillus fumigatus is a fungus with septate hyphae that branch at 45°, and conidia in radiating chains at ends of conidiophores. (web.app)
- Aspergillus fumigatus, a saprophytic fungus, can opportunistically cause a multitude of diseases in humans [2]. (web.app)
- Aspergillus- S--A.fumigatus-ak. (web.app)
Flavus5
- Identify key genes, using transcriptome analysis of Aspergillus flavus and Aspergillus flavus-crop interaction that are involved in fungal growth, morphogenesis, toxin production and virulence which can be used as targets for intervention strategies. (usda.gov)
- Identify metabolites produced by predicted secondary metabolic gene clusters in Aspergillus flavus, characterize the molecular regulation of their biosynthesis, and determine if they contribute to the fungus' ability to survive, colonizes host crops and produce aflatoxin. (usda.gov)
- Examine the role of climatic and environmental pressures on the growth, virulence, toxigenic potential, geographical distribution and aflatoxin production by Aspergillus flavus. (usda.gov)
- Aflatoxin contamination in crops such as corn, cottonseed, peanut, and tree nuts caused by Aspergillus (A.) flavus is a worldwide food safety problem. (usda.gov)
- a means of determining levels of activity of individual genes in organisms) to study the activity of all genes during the corn-Aspergillus (A.) flavus interaction. (usda.gov)
Gene3
- 3. Characterization of the Aspergillus nidulans septin (asp) gene family. (nih.gov)
- The putative methyltransferase LaeA is a global regulator that affects the expression of multiple secondary metabolite gene clusters in several fungi, and it can modify heterochromatin structure in Aspergillus nidulans. (oregonstate.edu)
- Using Aspergillus nidulans as a genetic model system, our group conducts basic research on gene function and regulation as well as the characterization of unconventional protein secretion and sugar uptake/utilization to generate knowledge that could be used for the optimization of the production and secretion of PCWDEs. (csic.es)
Emericella2
- Its teleomorph is Emericella nidulans. (nih.gov)
- Su teleomorfo es Emericella nidulans. (bvsalud.org)
Infections2
- Hyperbaric oxygen therapy may enhance antifungal efficacy in Aspergillus nidulans infections by promoting oxygen delivery and increasing immune response to inhibit fungal growth. (hbot-india.com)
- 11, 12 More research is needed about how Aspergillus becomes resistant and how to protect people from getting resistant Aspergillus infections. (web.app)
Fungal1
- This photomicrograph revealed some of the ultrastructural morphology exhibited by the fungal organism, Aspergillus nidulans . (cdc.gov)
Mold2
- ¹ Aspergillus is a common mold found in wet climates, on wood and is a primary decomposer of fallen leaves and vegetation. (vitacost.com)
- The mold that triggers the illnesses, aspergillus, is everywhere - indoors and outdoors. (web.app)
Species1
- Major pathogenic species of Aspergillus. (web.app)
Fungus1
- Aspergillus nidulans is a filamentous fungus frequently used as a model organism for genetic, biochemical, and molecular research, known for its easily manipulated genome and wide array of secondary metabolites. (hbot-india.com)
Main3
- Glucan, chitin and mannan are the main components of the Aspergillus nidulans hyphal cell wall. (uregina.ca)
- Environmental resistance selection is a main route for Aspergillus spp. (cdc.gov)
- Triazoles are the main class of drugs for treatment of aspergillus diseases. (cdc.gov)