A species of imperfect fungi from which the antibiotic fumigatin is obtained. Its spores may cause respiratory infection in birds and mammals.
A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.
Infections with fungi of the genus ASPERGILLUS.
A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.
An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.
A species of imperfect fungi which grows on peanuts and other plants and produces the carcinogenic substance aflatoxin. It is also used in the production of the antibiotic flavicin.
Hypersensitivity reaction (ALLERGIC REACTION) to fungus ASPERGILLUS in an individual with long-standing BRONCHIAL ASTHMA. It is characterized by pulmonary infiltrates, EOSINOPHILIA, elevated serum IMMUNOGLOBULIN E, and skin reactivity to Aspergillus antigen.
Reproductive bodies produced by fungi.
Infections of the respiratory tract with fungi of the genus ASPERGILLUS. Infections may result in allergic reaction (ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS), colonization in pulmonary cavities as fungus balls (MYCETOMA), or lead to invasion of the lung parenchyma (INVASIVE PULMONARY ASPERGILLOSIS).
Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.
A fungal toxin produced by various species of Trichoderma, Gladiocladium fimbriatum, Aspergillus fumigatus, and Penicillium. It is used as an immunosuppressive agent.
A triazole antifungal agent that inhibits cytochrome P-450-dependent enzymes required for ERGOSTEROL synthesis.
Microscopic threadlike filaments in FUNGI that are filled with a layer of protoplasm. Collectively, the hyphae make up the MYCELIUM.
Pulmonary diseases caused by fungal infections, usually through hematogenous spread.
Substances of fungal origin that have antigenic activity.
Proteins found in any species of fungus.
Lung infections with the invasive forms of ASPERGILLUS, usually after surgery, transplantation, prolonged NEUTROPENIA or treatment with high-doses of CORTICOSTEROIDS. Invasive pulmonary aspergillosis can progress to CHRONIC NECROTIZING PULMONARY ASPERGILLOSIS or hematogenous spread to other organs.
The ability of fungi to resist or to become tolerant to chemotherapeutic agents, antifungal agents, or antibiotics. This resistance may be acquired through gene mutation.
Macrolide antifungal antibiotic produced by Streptomyces nodosus obtained from soil of the Orinoco river region of Venezuela.
Five membered rings containing a NITROGEN atom.
Cyclic hexapeptides of proline-ornithine-threonine-proline-threonine-serine. The cyclization with a single non-peptide bond can lead them to be incorrectly called DEPSIPEPTIDES, but the echinocandins lack ester links. Antifungal activity is via inhibition of 1,3-beta-glucan synthase production of BETA-GLUCANS.
Deoxyribonucleic acid that makes up the genetic material of fungi.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Immunoglobulins produced in a response to FUNGAL ANTIGENS.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
Procedures for identifying types and strains of fungi.
Alkaloids originally isolated from the ergot fungus Claviceps purpurea (Hypocreaceae). They include compounds that are structurally related to ergoline (ERGOLINES) and ergotamine (ERGOTAMINES). Many of the ergot alkaloids act as alpha-adrenergic antagonists.
The ability of fungi to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance phenotype may be attributed to multiple gene mutations.
Infections of the nervous system caused by fungi of the genus ASPERGILLUS, most commonly ASPERGILLUS FUMIGATUS. Aspergillus infections may occur in immunocompetent hosts, but are more prevalent in individuals with IMMUNOLOGIC DEFICIENCY SYNDROMES. The organism may spread to the nervous system from focal infections in the lung, mastoid region, sinuses, inner ear, bones, eyes, gastrointestinal tract, and heart. Sinus infections may be locally invasive and enter the intracranial compartment, producing MENINGITIS, FUNGAL; cranial neuropathies; and abscesses in the frontal lobes of the brain. (From Joynt, Clinical Neurology, 1998, Ch 27, pp62-3)
Polysaccharides consisting of mannose units.
The functional hereditary units of FUNGI.
The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.
Toxic compounds produced by FUNGI.
The body of a fungus which is made up of HYPHAE.
The presence of bacteria, viruses, and fungi in the air. This term is not restricted to pathogenic organisms.
A family of 6-membered heterocyclic compounds occurring in nature in a wide variety of forms. They include several nucleic acid constituents (CYTOSINE; THYMINE; and URACIL) and form the basic structure of the barbiturates.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
An order of fungi in the phylum ASCOMYCOTA characterized by the presence of well defined peridia and cleistothecial asci. Notable anamorphs (mitosporic forms) of Eurotiales include PENICILLIUM and ASPERGILLUS.
Glucose polymers consisting of a backbone of beta(1->3)-linked beta-D-glucopyranosyl units with beta(1->6) linked side chains of various lengths. They are a major component of the CELL WALL of organisms and of soluble DIETARY FIBER.
A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.
Peptides whose amino and carboxy ends are linked together with a peptide bond forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS. Some of them are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL).
Furano-furano-benzopyrans that are produced by ASPERGILLUS from STERIGMATOCYSTIN. They are structurally related to COUMARINS and easily oxidized to an epoxide form to become ALKYLATING AGENTS. Members of the group include AFLATOXIN B1; aflatoxin B2, aflatoxin G1, aflatoxin G2; AFLATOXIN M1; and aflatoxin M2.
Animals that are generated from breeding two genetically dissimilar strains of the same species.
An imperfect fungus that produces ochratoxins and contaminates EDIBLE GRAIN and coffee beans.
Infection by a variety of fungi, usually through four possible mechanisms: superficial infection producing conjunctivitis, keratitis, or lacrimal obstruction; extension of infection from neighboring structures - skin, paranasal sinuses, nasopharynx; direct introduction during surgery or accidental penetrating trauma; or via the blood or lymphatic routes in patients with underlying mycoses.
A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).
An endocellulase with specificity for the hydrolysis of 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans including laminarin, paramylon, and pachyman.
Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)
A genus of zygomycetous fungi of the family Mucoraceae, order MUCORALES, a common saprophyte and facultative parasite of mature fruits and vegetables. It may cause cerebral mycoses in diabetes and cutaneous infection in severely burned patients.
A human or animal whose immunologic mechanism is deficient because of an immunodeficiency disorder or other disease or as the result of the administration of immunosuppressive drugs or radiation.
Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
Cell wall components constituting a polysaccharide core found in fungi. They may act as antigens or structural substrates.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A genus of ascomycetous fungi in the family Trichocomaceae, order EUROTIALES. Some species can cause opportunistic infections in humans, similar to its anamorph ASPERGILLUS.
Compounds consisting of a short peptide chain conjugated with an acyl chain.
The complete gene complement contained in a set of chromosomes in a fungus.
A defect of leukocyte function in which phagocytic cells ingest but fail to digest bacteria, resulting in recurring bacterial infections with granuloma formation. When chronic granulomatous disease is caused by mutations in the CYBB gene, the condition is inherited in an X-linked recessive pattern. When chronic granulomatous disease is caused by CYBA, NCF1, NCF2, or NCF4 gene mutations, the condition is inherited in an autosomal recessive pattern.
A linear polysaccharide of beta-1->4 linked units of ACETYLGLUCOSAMINE. It is the second most abundant biopolymer on earth, found especially in INSECTS and FUNGI. When deacetylated it is called CHITOSAN.
Substances found in PLANTS that have antigenic activity.
An NADPH-dependent P450 enzyme that plays an essential role in the sterol biosynthetic pathway by catalyzing the demethylation of 14-methyl sterols such as lanosterol. The enzyme acts via the repeated hydroxylation of the 14-methyl group, resulting in its stepwise conversion into an alcohol, an aldehyde and then a carboxylate, which is removed as formic acid. Sterol 14-demethylase is an unusual cytochrome P450 enzyme in that it is found in a broad variety of organisms including ANIMALS; PLANTS; FUNGI; and protozoa.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
A carcinogenic mycotoxin produced in high yields by strains of the common molds, Aspergillus versicolor, A. nidulans, and an unidentified species of Bipolaris. It causes necrosis of the liver and kidney and has an inhibitory effect on orotic acid incorporation into nuclear RNA.
A steroid of interest both because its biosynthesis in FUNGI is a target of ANTIFUNGAL AGENTS, notably AZOLES, and because when it is present in SKIN of animals, ULTRAVIOLET RAYS break a bond to result in ERGOCALCIFEROL.
A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.
The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.
A mitosporic Hypocreales fungal genus, various species of which are important parasitic pathogens of plants and a variety of vertebrates. Teleomorphs include GIBBERELLA.
A mitosporic fungal genus previously called Monosporium. Teleomorphs include PSEUDALLESCHERIA.
An enzyme that catalyzes the conversion of myo-inositol hexakisphosphate and water to 1L-myo-inositol 1,2,3,4,5-pentakisphosphate and orthophosphate. EC
An enzyme that catalyzes the hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of polysaccharide chains with the release of beta-glucose. It is also able to hydrolyze 1,6-alpha-glucosidic bonds when the next bond in sequence is 1,4.
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A genus of ascomycetous fungi, family Clavicipitaceae, order Hypocreales, parasitic on various grasses (POACEAE). The sclerotia contain several toxic alkaloids. Claviceps purpurea on rye causes ergotism.
A genus in the family Trichocomaceae, order EUROTIALES. The anamorph is ASPERGILLUS.
A genus of yeast-like mitosporic Saccharomycetales fungi characterized by producing yeast cells, mycelia, pseudomycelia, and blastophores. It is commonly part of the normal flora of the skin, mouth, intestinal tract, and vagina, but can cause a variety of infections, including CANDIDIASIS; ONYCHOMYCOSIS; vulvovaginal candidiasis (CANDIDIASIS, VULVOVAGINAL), and thrush (see CANDIDIASIS, ORAL). (From Dorland, 28th ed)
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

Association of a myosin immunoanalogue with cell envelopes of Aspergillus fumigatus conidia and its participation in swelling and germination. (1/1653)

A myosin immunoanalogue was identified in conidia of Aspergillus fumigatus by Western blotting, indirect immunofluorescence assay, and gold immunoelectron microscopy with two different antimyosin antibodies. The distribution pattern of this protein was followed during the early stages of germination. A single 180-kDa polypeptide, detected predominantly in a cell envelope extract, was found to cross-react with monoclonal and polyclonal antibodies raised against vertebrate muscle myosin. Immunoelectron microscopy permitted precise localization of this polypeptide, indicating that myosin analogue was mainly distributed along the plasma membrane of resting and swollen conidia. In germinating conidia, indirect immunofluorescence microscopy revealed myosin analogue at the periphery of germ tubes, whereas actin appeared as dispersed punctate structures in the cytoplasm that were more concentrated at the site of germ tube emergence. A myosin ATPase inhibitor, butanedione monoxime, greatly reduced swelling and blocked germination. In contrast, when conidia were treated with cytochalasin B, an inhibitor of actin polymerization, swelling was not affected and germination was only partially reduced. Butanedione monoxime-treated conidia showed accumulation of cytoplasmic vesicles and did not achieve cell wall reorganization, unlike swollen conidia. Collectively, these results suggest an essential role for this myosin analogue in the deposition of cell wall components during germination of A. fumigatus conidia and therefore in host tissue colonization.  (+info)

In vitro and in vivo activities of NS-718, a new lipid nanosphere incorporating amphotericin B, against Aspergillus fumigatus. (2/1653)

We evaluated the in vitro and in vivo potencies of a new lipid nanosphere that incorporates amphotericin B (AmB), NS-718, against Aspergillus fumigatus. The in vitro activity of NS-718 (the MIC at which 90% of strains are inhibited [MIC90], 0.25 microgram/ml) against 18 isolates of A. fumigatus was similar to that of deoxycholate AmB (D-AmB; Fungizone; MIC90, 0.25 microgram/ml), but NS-718 was more potent than liposomal AmB (L-AmB; AmBi-some; MIC90, 1.0 microgram/ml). The in vivo efficacy of NS-718 in a rat model of invasive pulmonary aspergillosis was compared with those of D-AmB and L-AmB. A low dose (1 mg/kg of body weight) of L-AmB was ineffective (survival rate, 0%), although equivalent doses of D-AmB and NS-718 were more effective (survival rate, 17%). However, a higher dose of NS-718 (3 mg/kg) was more effective (survival rate, 100%) than equivalent doses of D-AmB and L-AmB (survival rate, 0%). To explain these differences, pharmacokinetic studies showed higher concentrations of AmB in the plasma of rats treated with NS-718 than in the plasma of those treated with D-AmB. Our results suggest that NS-718, a new preparation of AmB, is a promising antifungal agent with activity against pulmonary aspergillosis.  (+info)

Amphotericin B- and fluconazole-resistant Candida spp., Aspergillus fumigatus, and other newly emerging pathogenic fungi are susceptible to basic antifungal peptides. (3/1653)

The present study shows that a number of basic antifungal peptides, including human salivary histatin 5, a designed histatin analog designated dhvar4, and a peptide from frog skin, PGLa, are active against amphotericin B-resistant Candida albicans, Candida krusei, and Aspergillus fumigatus strains and against a fluconazole-resistant Candida glabrata isolate.  (+info)

Early diagnosis of central nervous system aspergillosis with combination use of cerebral diffusion-weighted echo-planar magnetic resonance image and polymerase chain reaction of cerebrospinal fluid. (4/1653)

We treated a patient diagnosed as central nervous system (CNS) aspergillosis with the combined use of cerebral diffusion-weighted echo-planar magnetic resonance imaging (DWI) and polymerase chain reaction of the cerebrospinal fluid (CSF-PCR). DWI, a cutting-edge imaging modality to reveal the earliest changes of cerebral infarction, detected cerebral fungal embolization when the conventional computed tomographic scan and magnetic resonance imaging failed to reveal it. CSF-PCR demonstrated the presence of Aspergillus-specific DNA in the specimen, when the conventional examination and culture of CSF were nonspecific or negative. These diagnostic methods could be useful in the early diagnosis of CNS aspergillosis.  (+info)

Aspergillus meningitis: diagnosis by non-culture-based microbiological methods and management. (5/1653)

The performance of antibody detection, antigen detection, and Aspergillus genus-specific PCR for diagnosing Aspergillus meningitis was investigated with 26 cerebrospinal fluid (CSF) samples obtained from a single patient with proven infection caused by Aspergillus fumigatus. Immunoglobulin G antibodies directed against Aspergillus were not detected by enzyme-linked immunosorbent assay in CSF or serum. The antigen galactomannan was detected in the CSF 45 days before a culture became positive, and Aspergillus DNA was detected 4 days prior to culture. Decline of the galactomannan antigen titer in the CSF during treatment with intravenous and intraventricular amphotericin B and intravenous voriconazole corresponded with the clinical response to treatment.  (+info)

Contaminations occurring in fungal PCR assays. (6/1653)

Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed. The identities of all contaminants were specified by cycle sequencing and GenBank analysis. Twelve of 150 PCR assays that together included over 2,800 samples were found to be contaminated (3.3% of the negative controls were contaminated during the DNA extraction, and 4.7% of the PCR mixtures were contaminated during the amplification process). Contaminants were specified as Aspergillus fumigatus, Saccharomyces cerevisiae, and Acremonium spp. Further analysis showed that commercially available products like zymolyase powder or 10x PCR buffer may contain fungal DNA. In conclusion, the risk of contamination is not higher in fungal PCR assays than in other diagnostic PCR-based assays if general precautions are taken.  (+info)

Production of specific monoclonal antibodies to Aspergillus species and their use in immunohistochemical identification of aspergillosis. (7/1653)

Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining.  (+info)

Modulation of neutrophil-mediated activity against the pseudohyphal form of Candida albicans by granulocyte colony-stimulating factor (G-CSF) administered in vivo. (8/1653)

Renewed interest in neutrophil transfusions has emerged with the development and clinical use of granulocyte colony-stimulating factor (G-CSF). G-CSF not only increases neutrophil (polymorphonuclear leukocyte, PMNL) production but also modulates various physiological properties of PMNL. The effects of G-CSF on PMNL-mediated fungicidal activity were evaluated by administration of G-CSF (300 micrograms/day subcutaneously) to 5 healthy volunteers for 6 days. G-CSF significantly enhanced PMNL-mediated damage of Candida albicans pseudohyphae by 33% (P=.007) on day 2 and by 44% (P=.04) on day 6 at a 10:1 effector:target ratio. In contrast, the ability of PMNL to induce damage of hyphae from either Fusarium solani or Aspergillus fumigatus did not significantly change during the study period. These data demonstrate that G-CSF administered in vivo modulates PMNL-mediated fungicidal activity against the pseudohyphal form of C. albicans, thereby suggesting potential utility of G-CSF as a biologic response-modifying therapy in some opportunistic fungal infections.  (+info)

Stimulating encounter: The intimate, physical interaction between the soil-derived bacterium Streptomyces rapamycinicus and the human pathogenic fungus Aspergillus fumigatus led to the activation of an otherwise silent polyketide synthase (PKS) gene cluster coding for an unusual prenylated polyphenol (fumicycline A). The meroterpenoid pathway is regulated by a pathway-specific activator gene as well as by epigenetic factors.. ...
A. fumigatus strains and culture conditions: A. fumigatus strains used in this study are listed in Table 1. A. fumigatus strains were propagated at 37° on complete medium or minimal medium (MM) with 0.5 mm of one of the following nitrogen sources: sodium glutamate, ammonium tartrate, sodium nitrate, sodium nitrite, or hypoxanthine (Cove 1966). Uridine and uracil were added at a concentration of 5 mm when appropriate. Selection of A. fumigatus mutants unable to utilize nitrate as the sole nitrogen source was obtained by plating spores on MM containing ammonium tartrate and 100 mm sodium chlorate (Cove 1966). The nature of the mutation was assessed by growth on MM supplemented with different nitrogen sources, as previously described (Cove 1976). Selection of A. fumigatus mutants auxotrophic for uridine and uracil was achieved on MM containing 1 mg/ml 5-fluoroorotic acid plus uridine and uracil (dEnfert 1996). Liquid cultures used for DNA-mediated transformation and genomic DNA preparation were ...
Sphingolipids (SPLs) are key components of the plasma membrane in yeast and filamentous fungi. These molecules are involved in a number of cellular processes, and particularly, SGLs are essential components of the highly polarized fungal growth where they are required for the formation of the polarisome organization at the hyphal apex. Aspergillus fumigatus, a human fungal pathogen, produce SGLs that are discriminated into neutral cerebrosides, glycosylinositolphosphoceramides (GIPCs) and glycosylphosphatidylinositol (GPI) anchors. In addition to complex hydrophilic head groups of GIPCs, A. fumigatus is, to date, the sole fungus that produces a GPI-anchored polysaccharide. These SPLs follow three different biosynthetic pathways. Genetics blockage leading to the inhibition of any SPL biosynthesis or to the alteration of the structure of SPL induces growth and virulence defects. The complete lipid moiety of SPLs is essential for the lipid microdomain organization and their biosynthetic pathways are
Filamentous fungi represent classical examples for environmentally acquired human pathogens whose major virulence mechanisms are likely to have emerged long before the appearance of innate immune systems. In natural habitats, amoeba predation could impose a major selection pressure towards the acquisition of virulence attributes. To test this hypothesis, we exploited the amoeba Dicytostelium discoideum to study its interaction with Aspergillus fumigatus, two abundant soil inhabitants for which we found co-occurrence in various sites. Fungal conidia were efficiently taken up by D. discoideum, but ingestion was higher when conidia were devoid of the green fungal spore pigment DHN-melanin, in line with earlier results obtained for immune cells. Conidia were able to survive phagocytic processing and intracellular germination was initiated only after several hours of coincubation which eventually led to a lethal disruption of the host cell. Besides phagocytic interactions, both amoeba and fungus ...
Link to Pubmed [PMID] - 28677124. Mol. Microbiol. 2017 Sep;105(6):880-900. Aspergillus fumigatus, a ubiquitous human fungal pathogen, produces asexual spores (conidia), which are the main mode of propagation, survival and infection of this human pathogen. In this study, we present the molecular characterization of a novel regulator of conidiogenesis and conidial survival called MybA because the predicted protein contains a Myb DNA binding motif. Cellular localization of the MybA::Gfp fusion and immunoprecipitation of the MybA::Gfp or MybA::3xHa protein showed that MybA is localized to the nucleus. RNA sequencing data and a uidA reporter assay indicated that the MybA protein functions upstream of wetA, vosA and velB, the key regulators involved in conidial maturation. The deletion of mybA resulted in a very significant reduction in the number and viability of conidia. As a consequence, the ΔmybA strain has a reduced virulence in an experimental murine model of aspergillosis. RNA-sequencing and ...
The opportunistic human pathogen Aspergillus fumigatus initiates invasive growth through a programmed germination process that progresses from dormant spore to swollen spore (SS) to germling (GL) and ultimately invasive hyphal growth. We find a lipoxygenase with considerable homology to human Alox5 and Alox15, LoxB, that impacts the transitions of programmed spore germination. Overexpression of loxB (OE::loxB) increases germination with rapid advance to the GL stage. However, deletion of loxB (ΔloxB) or its signal peptide only delays progression to the SS stage in the presence of arachidonic acid (AA); no delay is observed in minimal media. This delay is remediated by the addition of the oxygenated AA oxylipin 5-hydroxyeicosatetraenoic acid (5-HETE) that is a product of human Alox5. We propose that A. fumigatus acquisition of LoxB (found in few fungi) enhances germination rates in polyunsaturated fatty acid-rich environments.
We present the genome sequences of a new clinical isolate of the important human pathogen, Aspergillus fumigatus, A1163, and two closely related but rarely pathogenic species, Neosartorya fischeri NRRL181 and Aspergillus clavatus NRRL1. Comparative genomic analysis of A1163 with the recently sequenced A. fumigatus isolate Af293 has identified core, variable and up to 2% unique genes in each genome. While the core genes are 99.8% identical at the nucleotide level, identity for variable genes can be as low 40%. The most divergent loci appear to contain heterokaryon incompatibility (het) genes associated with fungal programmed cell death such as developmental regulator rosA. Cross-species comparison has revealed that 8.5%, 13.5% and 12.6%, respectively, of A. fumigatus, N. fischeri and A. clavatus genes are species-specific. These genes are significantly smaller in size than core genes, contain fewer exons and exhibit a subtelomeric bias. Most of them cluster together in 13 chromosomal islands, ...
TY - JOUR. T1 - A Conserved C-Terminal Domain of the Aspergillus fumigatus Developmental Regulator MedA Is Required for Nuclear Localization, Adhesion and Virulence. AU - Al Abdallah, Qusai. AU - Choe, Se In. AU - Campoli, Paolo. AU - Baptista, Stefanie. AU - Gravelat, Fabrice N.. AU - Lee, Mark J.. AU - Sheppard, Donald C.. PY - 2012/11/21. Y1 - 2012/11/21. N2 - MedA is a developmental regulator that is conserved in the genome of most filamentous fungi. In the pathogenic fungus Aspergillus fumigatus MedA regulates conidiogenesis, adherence to host cells, and pathogenicity. The mechanism by which MedA governs these phenotypes remains unknown. Although the nuclear import of MedA orthologues has been reported in other fungi, no nuclear localization signal, DNA-binding domain or other conserved motifs have been identified within MedA. In this work, we performed a deletion analysis of MedA and identified a novel domain within the C-terminal region of the protein, designated MedA346-557, that is ...
The production of toxins by A. fumigatus may help the fungus to colonize and invade the respiratory epithelium by modifying the natural clearance of the respiratory tract. Previous research has shown that A. fumigatus culture filtrate modifies the transepithelial resistance (Rt) and transepithelial potential differences (Vt) of HNEC, an in vitro model of the air-liquid interface of airway epithelium [11]. The aim of this study was to use HPLC and MS-MS to identify which toxins produced by A. fumigatus are responsible for these modifications. Our data suggest that verruculogen, which has never been implicated in invasive aspergillosis, is one of the probable candidates.. The fact that A. fumigatus produces a number of biologically active substances that slow ciliary beating, damage epithelium, and that may affect colonization of the airways has already been reported using culture explants [17]. Among these substances, such toxins as gliotoxin, fumagillin, and helvolic acid have been implicated in ...
A new epimer of azaphilone derivative pinophilin B, epi-pinophilin B (1), and three known analogues (2-4) were obtained from the culture of the gorgonian-derived fungus Aspergillus fumigatus 14-27. The structures of 1-4, including their relative configurations were determined by extensive spectroscopic analysis and comparing with literature data. The absolute configuration of 1 was determined by electronic circular dichroism (ECD) and optical rotatory (OR) calculations methods. Compounds 1-4 were isolated from A. fumigatus for the first time. Their antibacterial and cytotoxic activities were also evaluated. PMID: 31564133 [PubMed - as supplied by publisher]. Source: Industry. ...
Melanins, or melanin-like compounds, may play a role in the pathogenesis of a number of human fungal infections. This study investigated the production of melanin by the important opportunistic pathogen Aspergillus fumigatus. Conidia from A. fumigatus were harvested and treated with proteolytic enzymes, denaturant and hot, concentrated acid; this yielded dark particles which were similar in size and shape to the original propagules. Electron spin resonance spectroscopy revealed that the conidial-derived particles were stable free radicals consistent with an identification as melanin. Melanin particles were used to immunize BALB/c mice in order to produce a total of five anti-melanin monoclonal antibodies (mAbs). The latter mAbs were strongly reactive both with intact conidia and with extracted melanin particles by ELISA and immunofluorescence reactivity. Immunofluorescence labelling with the novel mAbs was used to examine the temporal expression of melanin during in vitro culture of A. fumigatus
TY - JOUR. T1 - Biosynthesis of β-(1→5)-galactofuranosyl chains of fungal-type and o-mannose-type galactomannans within the invasive pathogen aspergillus fumigatus. AU - Chihara, Yuria. AU - Tanaka, Yutaka. AU - Izumi, Minoru. AU - Hagiwara, Daisuke. AU - Watanabe, Akira. AU - Takegawa, Kaoru. AU - Kamei, Katsuhiko. AU - Shibata, Nobuyuki. AU - Ohta, Kazuyoshi. AU - Oka, Takuji. PY - 2020/1/1. Y1 - 2020/1/1. N2 - ABSTRACT The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes. Galactomannans comprise α-(1→2)-/α-(1→6)-mannan and β-(1→5)-/ β-(1→6)-galactofuranosyl chains. We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains. In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus. Two paralogs exist within A. ...
Land, C.J.; Sostarić, B.; Fuchs, R.; Lundström, H.; Hult, K., 1989: Intratracheal exposure of rats to Aspergillus fumigatus spores isolated from sawmills in Sweden
In this experiment, the OD value of different concentrations of Aspergillus fumigatus cultured for 48 hours was measured on a microplate reader using a 96-well plate. The absorbance-concentration curve was plotted according to the results, and the absorbance value was used instead of visual observation to quickly detect the growth of Aspergillus fumigatus. situation. The results showed that the OD value was between 0.05 and 0.06 when visually 50%, and 100% when the OD value was greater than 0.17. The use of the OD value can be a rapid method for observing the growth of Aspergillus fumigatus, which has the advantages of high efficiency, accuracy and high throughput compared with the visual method.. ...
The pharmacodynamics (PD) of liposomal amphotericin B (L-AMB) remain relatively poorly understood because of complex pharmacokinetics (PK) that impede the in-depth comprehension of its exposure-response relationship (1). Although high L-AMB doses up to 15 mg/kg have been used (2), in the absence of a clinical dose-response relationship a dose of 3 mg/kg is generally recommended for the treatment of invasive aspergillosis (IA) (1), with an end-of-treatment favorable response of ∼40% for probable/proven cases (3). However, neutropenia may affect the clinical response to L-AMB therapy (4). We therefore studied L-AMB PD in an in vitro PK/PD model using previously published data of experimental aspergillosis in neutropenic and nonneutropenic animal models and optimized L-AMB therapy simulating human serum concentration-time profiles against azole-susceptible and azole-resistant Aspergillus fumigatus isolates in neutropenic and nonneutropenic patients.. Two clinical A. fumigatus isolates, a ...
Glycoinositolphosphoceramides (GIPCs) are complex sphingolipids present at the plasma membrane of various eukaryotes with the important exception of mammals. In fungi, these glycosphingolipids commonly contain an alpha-mannose residue (Man) linked at position 2 of the inositol. However, several pathogenic fungi additionally synthesize zwitterionic GIPCs carrying an alpha-glucosamine residue (GlcN) at this position. In the human pathogen Aspergillus fumigatus, the GlcNalpha1,2IPC core (where IPC is inositolphosphoceramide) is elongated to Manalpha1,3Manalpha1,6GlcNalpha1,2IPC, which is the most abundant GIPC synthesized by this fungus. In this study, we identified an A. fumigatus N-acetylglucosaminyltransferase, named GntA, and demonstrate its involvement in the initiation of zwitterionic GIPC biosynthesis. Targeted deletion of the gene encoding GntA in A. fumigatus resulted in complete absence of zwitterionic GIPC; a phenotype that could be reverted by episomal expression of GntA in the mutant. ...
Host-pathogen interactions have critical implications for the establishment of disease and for determining adaptive immune responses of the host. This study has conducted global Aspergillus fumigatus transcriptional analyses throughout the initiation of murine infection using a wild-type and an attenuated ΔlaeA isolate. A novel data analysis protocol was applied from which three time-series datasets were generated between 4, 8 and 14 hours post infection. This approach identified distinct temporal gene expression profiles during disease initiation whereby numerous secreted enzymes, including proteases and antigens, were upregulated between 4 and 8 hours, while a striking upregulation of genes in secondary metabolism clusters and subtelomeric loci was observed between 8 and 14 hours. In order to test the role of several upregulated secondary metabolite genes on host-pathogen interactions and virulence, two isolates mutated in non-ribosomal peptide synthetase encoding genes (ΔftmA, Δpes3), and ...
The opportunistic fungal pathogen Aspergillus fumigatus is the causative agent of more than 90% of all Invasive Aspergillosis (IA) infections diagnosed in immun...
Abstract Sulphur is an essential element that all pathogens have to absorb from their surroundings in order to grow inside their infected host. Despite its importance, the relevance of sulphur assimilation in fungal virulence is largely unexplored. Here we report a role of the bZIP transcription factor MetR in sulphur assimilation and virulence of the human pathogen Aspergillus fumigatus. The MetR regulator is essential for growth on a variety of sulphur sources; remarkably, it is fundamental for assimilation of inorganic S-sources but dispensable for utilization of methionine. Accordingly, it strongly supports expression of genes directly related to inorganic sulphur assimilation but not of genes connected to methionine metabolism. On a broader scale, MetR orchestrates the comprehensive transcriptional adaptation to sulphur-starving conditions as demonstrated by digital gene expression analysis. Surprisingly, A. fumigatus is able to utilize volatile sulphur compounds produced by its methionine ...
1. KornitzerD 2009 Fungal mechanisms for host iron acquisition. Current opinion in microbiology 12 377 383. 2. SchrettlMBeckmannNVargaJHeinekampTJacobsenID 2010 HapX-mediated adaption to iron starvation is crucial for virulence of Aspergillus fumigatus. PLoS Pathog 6 e1001124 doi:10.1371/journal.ppat.1001124. 3. SchrettlMIbrahim-GranetODroinSHuerreMLatgeJP 2010 The crucial role of the Aspergillus fumigatus siderophore system in interaction with alveolar macrophages. Microbes and infection/Institut Pasteur 12 1035 1041. 4. SchrettlMBignellEKraglCSabihaYLossO 2007 Distinct roles for intra- and extracellular siderophores during Aspergillus fumigatus infection. PLoS Pathog 3 e128 doi:10.1371/journal.ppat.0030128. 5. SchrettlMBignellEKraglCJoechlCRogersT 2004 Siderophore biosynthesis but not reductive iron assimilation is essential for Aspergillus fumigatus virulence. The Journal of experimental medicine 200 1213 1219. 6. HissenAHChowJMPintoLJMooreMM 2004 Survival of Aspergillus fumigatus in serum ...
While azole drugs targeting the biosynthesis of ergosterol are effective antifungal agents, their extensive use has led to the development of resistant organisms. Infections involving azole resistant forms of the filamentous fungus Aspergillus fumigatus are often associated with genetic changes in the cyp51A gene encoding the lanosterol α14 demethylase target enzyme. Both a sequence duplication in the cyp51A promoter (TR34) as well as a substitution mutation in the coding sequence (L98H) are required for full expression of azole resistance. A mechanism commonly observed in pathogenic yeast such as Candida albicans involves gain-of-function mutations in transcriptional regulatory proteins that induce expression of ATP-binding cassette (ABC) transporter encoding genes. We and others have found that an ABC transporter protein called Cdr1B (here referred to as AbcG1) is required for wild-type azole resistance in A.fumigatus Here we test the genetic relationship between the TR34 L98H allele of ...
Aspergillus fumigatus ATCC ® 1022D-2™ Designation: Genomic DNA from Aspergillus fumigatus Strain 118 [ATCC ® 1022™] Application:
Aspergillus fumigatus Z5 has a strong ability to decompose lignocellulose biomass, and its extracellular protein secretion has been reported in earlier studies employing traditional techniques. However, a comprehensive analysis of its secretion in the presence of different carbon sources is still lacking. The goal of this work was to identify, quantify and compare the secretome of A. fumigatus Z5 in the presence of different carbon sources to understand in more details the mechanisms of lignocellulose decomposition by Aspergillus fumigatus Z5. Cellulolytic A. fumigatus Z5 was grown in the presence of glucose (Gl), Avicel (Av) and rice straw (RS), and the activities of several lignocellulosic enzymes were determined with chromatometry method. The maximum activities of endoglucanase, exoglucanase, β-glucosidase, laminarinase, lichenase, xylanase and pectin lyase were 12.52, 0.59, 2.30, 2.37, 1.68, 15.02 and 11.40 U·ml-1, respectively. A total of 152, 125 and 61 different proteins were identified in the
Filamentous fungi are an important cause of pulmonary and systemic morbidity and mortality, and also cause corneal blindness and visual impairment worldwide. Utilizing in vitro neutrophil killing assays and a model of fungal infection of the cornea, we demonstrated that Dectin-1 dependent IL-6 production regulates expression of iron chelators, heme and siderophore binding proteins and hepcidin in infected mice. In addition, we show that human neutrophils synthesize lipocalin-1, which sequesters fungal siderophores, and that topical lipocalin-1 or lactoferrin restricts fungal growth in vivo. Conversely, we show that exogenous iron or the xenosiderophore deferroxamine enhances fungal growth in infected mice. By examining mutant Aspergillus and Fusarium strains, we found that fungal transcriptional responses to low iron levels and extracellular siderophores are essential for fungal growth during infection. Further, we showed that targeting fungal iron acquisition or siderophore biosynthesis by ...
Aspergillus fumigatus is the most common airborne fungal pathogen of humans, causing life-threatening invasive disease in immunocompromised patients. The limitations of therapeutic intervention are reflected in mortality rates, dependent primarily on the immune status of the host, of up to 90% (1, 2). The importance of the host immune status has been underlined by the lack of identified specific virulence attributes in A. fumigatus (1, 3-6). Limiting access to essential nutrients is an often overlooked aspect of innate immunity (7). Iron is essential for most organisms, suggesting that its acquisition in vivo may be required for A. fumigatus to cause disease (8-10). Iron uptake systems are often required for bacterial and yeast virulence (11, 12).. Fungi have evolved various strategies, often used in parallel, to acquire iron. These include two high affinity uptake mechanisms, reductive iron assimilation, and siderophore-assisted iron mobilization (8-10). Siderophores are low molecular mass, ...
Invasive aspergillosis is a life-threatening disease, and its incidence has increased in the recent past. Dectin-1 recognizes β-glucans and mediates innate immune responses to Aspergillus fumigatus. Transcription factor PU.1 has been the focus of recent research due to its role in inflammation and infection. However, its role in Dectin-1 regulation during A. fumigatus infection remains to be elucidated. THP-1 cells were stimulated with A. fumigatus conidia. We then used real-time RT-PCR, Western blot, and immunofluorescence assays to analyze the mRNA and protein levels and cellular distribution, respectively, of Dectin-1 and PU.1 in stimulated THP-1 cells. Additionally, we used the luciferase reporter assays, chromatin immunoprecipitation (ChIP) assays, electrophoretic mobility shift assays (EMSA), and RNA interference experiments to investigate the role of PU.1 in Dectin-1 regulation. Our results revealed that Dectin-1 mRNA and protein levels as well as the PU.1 protein level were increased in THP-1
Aspergillus fumigatus is considered a common causative agent of human fungal infections. A restricted number of virulence factors have been described, and none of them lead to a differentiation in the virulence level among different strains. Variations in the virulence phenotype depending on the isolate origin, measured as survival percentage in animal infection models, have been previously reported. In this study, we analyzed the whole-genome sequence of A. fumigatus isolates from clinical and environmental origins to determine their virulence genetic content. The sample included four isolates sequenced at the University Medical Center Groningen (UMCG), three clinical (two of them isolated from the same patient) and the experimental strain B5233, and the draft genomes of one reference strain, two environmental and two clinical isolates obtained from a public database. The fungal genomes were screened for the presence of virulence-related genes (VRGs) using an in-house database of 244 genes related to
Sensitization to Aspergillus fumigatus as a risk factor for bronchiectasis in COPD Stephanie Everaerts,1,2 Katrien Lagrou,3,4 Adriana Dubbeldam,5 Natalie Lorent,1 Kristina Vermeersch,2 Erna Van Hoeyveld,3 Xavier Bossuyt,3,4 Lieven J Dupont,1,2 Bart M Vanaudenaerde,2 Wim Janssens1,2 1Department of Respiratory Diseases, University Hospitals Leuven, 2Laboratory of Respiratory Diseases, Department of Clinical and Experimental Medicine, KU Leuven, 3Department of Laboratory Medicine, University Hospitals Leuven, 4Department of Microbiology and Immunology, KU Leuven, 5Department of Radiology, University Hospitals Leuven, Leuven, Belgium Background: Bronchiectasisâ chronic obstructive pulmonary disease (COPD) overlap presents a possible clinical phenotype of COPD, but it is unclear why it develops in a subset of patients. We hypothesized that sensitization to Aspergillus fumigatus (A fum) is associated with bronchiectasis in COPD and occurs more frequently in vitamin D-deficient patients.Methods: This
Aspergillus fumigatus is a thermotolerant human-pathogenic mold and the most common cause of invasive aspergillosis (IA) in immunocompromised patients. Its predominance is based on several factors most of which are still unknown. The thermotolerance of A. fumigatus is one of the traits which have been assigned to pathogenicity. It allows the fungus to grow at temperatures up to and above that of a fevered human host. To elucidate the mechanisms of heat resistance, we analyzed the change of the A. fumigatus proteome during a temperature shift from 30°C to 48°C by 2D-fluorescence difference gel electrophoresis (DIGE). To improve 2D gel image analysis results, protein spot quantitation was optimized by missing value imputation and normalization. Differentially regulated proteins were compared to previously published transcriptome data of A. fumigatus. The study was augmented by bioinformatical analysis of transcription factor binding sites (TFBSs) in the promoter region of genes whose corresponding
Pseudomonas aeruginosa and Aspergillus fumigatus are the two microorganisms responsible for most of the chronic infections in cystic fibrosis patients. P.
Metal restriction imposed by mammalian hosts during an infection is a common mechanism of defence to reduce or avoid the pathogen infection. Metals are essential for organism survival due to its involvement in several biological processes. Aspergillus fumigatus causes invasive aspergillosis, a disea …
Invasive aspergillosis is a life-threatening and difficult to treat infection in immunosuppressed patients. The efficacy of current anti-Aspergillus therapies, targeting the cell wall or membrane, is limited by toxicity (polyenes), fungistatic activity and some level of basal resistance (echinocandins), or the emergence of acquired resistance (triazoles). The heat shock protein 90 (Hsp90) is a conserved molecular chaperone involved in the rapid development of antifungal resistance in the yeast Candida albicans. Few studies have addressed its role in filamentous fungi such as Aspergillus fumigatus, in which mechanisms of resistance may differ substantially. Hsp90 is at the center of a complex network involving calcineurin, lysine deacetylases (KDAC) and other client proteins, which orchestrate compensatory repair mechanisms of the cell wall in response to the stress induced by antifungals. In A. fumigatus, Hsp90 is a trigger for resistance to high concentrations of caspofungin, known as
Modern medical technologies are repairing the human body in ways never imagined only a few years ago, but they are leaving an increasing population of patients who are newly susceptible to opportunistic pathogens. Invasive and chronic fungal infections have become a formidable clinical opponent, and foremost among them is Aspergillus fumigatus. Aspergillusfumigatus and Aspergillosis assembles chapters from a large and international contingent of experts in the field to explore every major aspect of A. fumigatus and how it kills so many patients. This volume offers the latest insights into the fundamental biology and pathogenesis of A. fumigatus and how it establishes disease, as well as the newest strategies for characterizing, diagnosing, and treating its spectrum of clinical infection. This valuable book is an instrumental resource for both scientists and clinicians tackling the current problems with Aspergillosis. It presents chapters on the species itself, including morphology and unique and
Purpose: Calcineurin orchestrates growth, stress responses and virulence in major pathogenic fungi including Aspergillus fumigatus responsible for life-threatening fungal infections worldwide. While these cellular regulatory functions of calcineurin make it an attractive antifungal target, the immunosuppressive effects of the currently available calcineurin inhibitors, FK506 and CsA, make it difficult to exploit the antifungal potential due to conservation of calcineurin in the host and the fungal pathogen. Critical molecular understanding of calcineurin-immunophilin-immunosuppressor complexes would facilitate the design of novel non-immunosuppressive CsA and FK506 analogs for fungal-specific targeting of calcineurin.. Methods: We solved the crystal structure of calcineurin-FK506-FKBP12 complex in A. fumigatus and using site-directed mutagenic approaches, we constructed several mutations in the CnaA catalytic subunit of calcineurin and FKBP12. To identify differences between the A. fumigatus ...
Personal exposures to A. fumigatus are associated with a variety of adverse health outcomes, including invasive aspergillosis, allergic sensitization, and asthma. Due to the high rate of mortality associated with invasive disease in immunocompromised patients, most studies of A. fumigatus have aimed to characterize the immune responses in immunocompromised murine models. However, a larger portion of the population is affected by fungal-induced allergies and asthma and the immune mechanisms associated with exposure have rarely been studied in an immunocompetent model. These models do not accurately reflect the natural method of exposure to environmental sources of conidia, and may significantly impact responses between fungi and the host immune system. Furthermore, little is known about the mechanisms associated with fungal induced allergy and asthma. Persistence of antigen is believed to play a role in induction of these diseases. Melanin, an A. fumigatus virulence factor, protects conidia from innate
The SREBP SrbA in A. fumigatus has previously been shown to be required for hypoxia and low-iron adaptation, triazole drug susceptibility, and virulence (4, 5, 47). Given the potential importance of SrbA in fungal virulence and responses to antifungal therapy, a further examination of the mechanisms of SrbA regulation and function is warranted in A. fumigatus. SREBPs exist as precursor proteins that are cleaved to release the active N terminus transcription factor (8). In A. fumigatus, whether SrbA required proteolytic processing for function as a transcription factor was unclear. Bioinformatic analyses of the SrbA amino acid sequence strongly suggest that SrbA is an integral membrane protein like SREBPs in other organisms. Here, we observed that the GFP signal from an SrbA:GFP fusion protein strain largely localizes to the nuclear envelope/ER membrane, supporting the bioinformatics data with regard to SrbA localization in A. fumigatus. However, previous attempts to identify the sterol cleavage ...
2005. The Aspergillus nidulans phytochrome FphA represses sexual development in red light. Curr. Biol. 15:1833-1838. Casselton, L. A. 2002. Mate recognition in fungi. Heredity 88:142- 147. , J. P. Debeaupuis, J. Sarfati, J. Lortholary, P. Ribaud, P. Shah, M. Cornet, H. V. Thien, E. Gluckman, G. Bru ¨cker, and J. P. Latge´. 1998. Molecular typing of environmental and patient isolates of Aspergillus fumigatus from various hospital settings. J. Clin. Microbiol. 36:1494-1500. Debeaupuis, J. , J. Sarfati, V. Momany, T. Tanaka, T. Kumagai, K. Asai, M. Machida, W. C. Nierman, D. W. Denning, M. Caddick, M. Hynes, M. Paoletti, R. Fischer, B. Miller, P. Dyer, M. S. Sachs, S. A. Osmani, and B. W. Birren. 2005. Sequencing and comparative analysis of Aspergillus nidulans. Nature 438:1105-1115. , M. Christensen, A. H. Onions, J. I. Pitt, and R. A. Samson. 1985. Infrageneric taxa of Aspergillus, p. 55-62. In R. A. Samson and J. I. ), Advances in Penicillium and Aspergillus Systematics. Plenum Press, New ...
Aspergillus fumigatus is the main cause of invasive aspergillosis in immunocompromised patients, and only a limited number of drugs for treatment are available. A screening method for new antifungal compounds is urgently required, preferably an appro
Objective: To report the cases of 6 patients with fungus ball caused by Aspergillus fumigatus (aspergilloma) in the pleural cavity. Methods: Between 1980 and 2009, 391 patients were diagnosed with aspergilloma at the Santa Casa Hospital Complex in Porto Alegre, Brazil. The diagnosis of aspergilloma in the pleural cavity was made through imaging tests revealing effusion and pleural thickening with air-fluid level; direct mycological examination revealing septate hyphae, consistent with Aspergillus sp.; and positive culture for A. fumigatus in the surgical specimen from the pleural cavity. Results: Of the 391 patients studied, 6 (2%) met the established diagnostic criteria. The mean age of those 6 patients was 48 years (range, 29-66 years), and 5 (83%) were male. The most common complaints were cough, expectoration, and hemoptysis. Four patients (67%) had a history of tuberculosis that had been clinically cured. All of the patients were submitted to surgical removal of the aspergilloma, followed ...
Hyperspectral imaging (HSI) is a technique based on the combination of classical spectroscopy and conventional digital image processing. It is also well suited for the biological assays and quantitative real-time analysis since it provides spectral and spatial data of samples. The method grants detailed information about a sample by recording the entire spectrum in each pixel of the whole image. We applied HSI to quantify the constituent pH variation in a single infected apoptotic monocyte as a model system. Previously, we showed that the human-pathogenic fungus Aspergillus fumigatus conidia interfere with the acidification of phagolysosomes. Here, we extended this finding to monocytes and gained a more detailed analysis of this process. Our data indicate that melanised A. fumigatus conidia have the ability to interfere with apoptosis in human monocytes as they enable the apoptotic cell to recover from mitochondrial acidification and to continue with the cell cycle. We also showed that this ability of A
Aspergillus fumigatus is a well-known opportunistic pathogen that causes invasive aspergillosis (IA) infections with high mortality in immunosuppressed individuals. Morphogenesis, including hyphal growth, conidiation, and cell wall biosynthesis is crucial in A. fumigatus pathogenesis. Based on a previous random insertional mutagenesis library, we identified the putative polysaccharide synthase gene Afcps1 and its para-log Afcps2. Homologs of the cps gene are commonly found in the genomes of most fungal and some bacterial pathogens. Afcps1/cpsA is important in sporulation, cell wall composition, and virulence. However, the precise regulation patterns of cell wall integrity by Afcps1/cpsA and further effects on the immune response are poorly understood. Specifically, our in-depth study revealed that Afcps1 affects cell-wall stability, showing an increased resistance of ΔAfcps1 to the chitinmicrofibril destabilizing compound calcofluor white (CFW) and susceptibility of ΔAfcps1 to the ...
could get genotypically -,,Jacques Meis: Yes.,,Christine Ginocchio: - with the resistance. Okay.,,Jacques Meis: At least in Europe. Im not sure what the epidemiology is here. Probably, you have also a lot of -,,Tom Chiller: We dont - we dont ...
Aspergillus fumigatus Basics Laboratory Metabolites Adverse health reactions Specific settings Diagnostic Bibliography Basics A. fumigatus has been associated with each and every type of health problems linked to environmental moulds: irritation and inflammation, allergy, asthma, pneumonitis, toxic effects as well as a wide range of infections. Infections have been reported
Fungal keratitis is a kind of intractable and sight-threatening diseases. Spleen-tyrosine kinase (Syk) is a non-receptor tyrosine kinase, which plays an important role in the signaling pathway of the receptors. In the current study, we investigate the expression and function of Syk in human corneal epithelial cells with Aspergillus fumigatus (A. fumigatus) infection. Cultured telomerase-immortalized human corneal epithelial cells (THCEs) were treated with A. fumigatus hyphae with or without treatment of Syk inhibitors. Activation of Syk and the role of Syk in regulating inflammatory cytokines and chemokines expression were evaluated. The mRNA expression was determined by real time PCR, and protein activation was measured by western blotting. Syk protein was detected in THCEs, and its activation was enhanced after treatment of A. fumigatus hyphae. Expression of inflammatory cytokines (IL-1β and IL-6) and chemokines (IL-8 and CXCL1) mRNA were significantly increased after stimulation of A. fumigatus
Due to the increase in the number of azole-resistant Aspergillus fumigatus, there is an urgent need of data to predict future trends and prevent further spreading. The intercountry transfer of resistant A. fumigatus on plant bulbs have been reported. We investigated existence and characteristics of resistant isolates attached to agricultural products imported to Japan. We purchased 292 samples in Japan. All samples were screened for the existence of azole-resistant A. fumigatus. For positive isolates, minimum inhibitory concentrations of the drugs were determined. We also analyzed Cyp51A, Hmg1, and Erg6 mutations of these isolates and conducted microsatellite genotyping. Fourteen azole-resistant isolates were detected, of which 13 were cultured from flower bulbs imported from the Netherlands. Among them 5 were from 11 bulbs of Hippeastrum (45.5%), 5 were from 24 bulbs of Gladiolus (20.8%), 2 were from 4 bulbs of Ixia (50.0%), and 1 was from 22 bulbs of Tulipa (4.5%). Only 1 resistant isolate was ...
The emergence of azole-resistant Aspergillus fumigatus has become a clinical problem in many parts of the world. Several amino acid mutations in the azole target protein Cyp51Ap contribute to this resistance, with the most concerning being the environmentally derived TR34 /L98H and TR46 /Y121F/T289A mutations. Here, we performed passive surveillance to assess a sample of the A. fumigatus population in the United States for the presence of these mutations. We found 1.4% of those isolates to exhibit elevated MIC via broth microdilution, and five of those isolates harbored the TR34 /L98H mutation ...
Review question We planned to review evidence about the effect of treatments to fight fungal infections which cause allergic bronchopulmonary aspergillosis in people with cystic fibrosis.. Background Allergic bronchopulmonary aspergillosis (ABPA) is an allergic lung reaction to a type of fungus (usually Aspergillus fumigatus) in some people with cystic fibrosis. It causes cough and wheezing and sometimes fever. If left untreated ABPA can lead to chronic lung damage. It is usually treated with a high dose of corticosteroids (also known as steroids). However, it has not been proven that corticosteroids can prevent lung function deteriorating in the long term. Also, long-term use of steroids is linked to some serious side effects. Treating the fungus which causes ABPA may be an alternative to using high doses of steroids to combat the allergic reaction. This is an update of a previously published review.. Search date The evidence is current to: 29 September 2016.. Study characteristics No trials ...
Allergic Bronchopulmonary Aspergillosis (ABPA): Symptoms ❗ Workup ❗ Diagnosis ❗ Treatment ❗ Complications ❗ Causes ❗ Epidemiology ❗ Incidence ❗ Prognosis ❗ Check at SYMPTOMA.com Allergic bronchopulmonary aspergillosis is a disease characterized by a hypersensitivity reaction to aspergillus fumigatus after its repeated inhalation and is most…
BioAssay record AID 322857 submitted by ChEMBL: Antifungal activity against azole-resistant Aspergillus fumigatus V34/75-CM3276 isolate from patient with hyper-immunoglobulin E syndrome by broth microdilution susceptibility test.
TY - JOUR. T1 - Damage to Aspergillus fumigatus and Rhizopus oryzae hyphae by oxidative and nonoxidative microbicidal products of human neutrophils in vitro. AU - Diamond, R. D.. AU - Clark, R. A.. PY - 1982. Y1 - 1982. N2 - Our previous studies established that human neutrophils could damage and probably kill hyphae of Aspergillus fumigatus and Rhizopus oryzae in vitro, primarily by oxygen-dependent mechanisms active at the cell surface. These studies were extended, again quantitating hyphal damage by reduction in uptake of 14C-labeled uracil or glutamine. Neither A. fumigatus nor R. oryzae hyphae were damaged by neutrophils from patients with chronic granulomatous disease, confirming the importance of oxidative mechanisms in damage to hyphae. In contrast, neutrophils from one patient with hereditary myeloperoxidase deficiency damaged R.C. oryzae but not A. fumigatus hyphae. Cell-free, in vitro systems were then used to help determine the relative importance of several potentially fungicidal ...
Caspofungin is a member of the echinocandin class of antifungal agents that inhibit the synthesis of β 1,3 glucan thus disrupting fungal cell wall structure and function. Exposure of the Aspergillus fumigatus cultures to caspofungin (0.01, 0.1 or 1.0 μ g/ml) resulted in a reduction in cell growth, but the production of the epipolythiodioxopiperazine toxin, gliotoxin, was comparable, or greater, in cultures exposed to caspofungin than untreated controls. Exposure of A. fumigatus hyphae to 1.0 μ g/ml caspofungin for 4 h resulted in the release of amino acids ( P 0.01), protein ( P 0.002) and gliotoxin ( P 0.02). Cultures of A. fumigatus incubated in the presence of caspofungin for 4 or 24 h demonstrated enhanced gliotoxin release ( P 0.04 and 0.03, respectively) and biosynthesis ( P 0.04 and 0.03, respectively) compared to that by control cultures. The results presented here indicate that exposure of A. fumigatus to caspofungin results in increased cell permeability and an increase in the ...
Allergic bronchopulmonary aspergillosis (ABPA) is a pulmonary disorder caused by a complex hypersensitivity response to antigens released by the fungus Aspergillus fumigatus. The management of ABPA includes two important aspects - institution of immunosuppressive therapy in the form of glucocorticoids to control the immunologic activity, and close monitoring for detection of relapses. Another possible target is to use antifungal agents to attenuate the fungal burden secondary to the fungal colonization in the airways. Oral corticosteroids are currently the treatment of choice for ABPA associated with bronchial asthma.They not only suppress the immune hyperfunction but are also anti-inflammatory. However, there is no data to guide the dose and duration of glucocorticoids and different regimens of glucocorticoids have been used in literature.Itraconazole, an oral triazole with relatively low toxicity, is active against Aspergillus spp. in vitro and in vivo. The activity of itraconazole against ...
TY - JOUR. T1 - Allergic bronchopulmonary aspergillosis treated successfully for one year with omalizumab. AU - Collins, Jennifer. AU - de Vos, Gabriele. AU - Hudes, Golda. AU - Rosenstreich, David. PY - 2012. Y1 - 2012. N2 - Background: Current therapy for allergic bronchopulmonary aspergillosis (ABPA) uses oral corticosteroids, exposing patients to the adverse effects of these agents. There are reports of the steroid-sparing effect of anti-IgE therapy with omalizumab for ABPA in patients with cystic fibrosis (CF), but there is little information on its efficacy against ABPA in patients with bronchial asthma without CF. Objective: To examine the effects of omalizumab, measured by asthma control, blood eosinophilia, total serum immunoglobulin E (IgE), oral corticosteroid requirements, and forced expiratory volume spirometry in patients with ABPA and bronchial asthma. Methods: A retrospective review of charts from 2004-2006 of patients treated with omalizumab at an academic allergy and immunology ...
This study describes a sudden death in an ostrich (Struthio camelus) with a big neck. Grossly, the air sacs were thickened. Yellow to white, round or coalescent material was scattered on the air sacs. However, the cervical air sac was normal. Histopathologically, multinucleated giant cells, heterophils, and macrophages had infiltrated the air sacs, and many hyphae were seen in the air sacs and on their surfaces. Pyogranulomatous inflammation with intralesional hyphae was observed throughout the lungs. Aspergillus fumigatus was identified microbiologically. In conclusion, the affected animal died because of the respiratory disturbance caused by mycotic airsacculitis and pneumonia.
Pseudomonas aeruginosa and Aspergillus fumigatus are common opportunistic bacterial and fungal pathogens, respectively. They often coexist in airways of immunocompromised patients and individuals with cystic fibrosis, where they form biofilms and cause acute and chronic illnesses. Hence, the interactions between them have long been of interest and it is known that P. aeruginosa can inhibit A. fumigatusin vitro We have approached the definition of the inhibitory P. aeruginosa molecules by studying 24 P. aeruginosa mutants with various virulence genes deleted for the ability to inhibit A. fumigatus biofilms. The ability of P. aeruginosa cells or their extracellular products produced during planktonic or biofilm growth to affect A. fumigatus biofilm metabolism or planktonic A. fumigatus growth was studied in agar and liquid assays using conidia or hyphae. Four mutants, the pvdD pchE, pvdD, lasR rhlR, and lasR mutants, were shown to be defective in various assays. This suggested the P. aeruginosa ...
Allergic bronchopulmonary aspergillosis (ABPA) is a condition characterised by an exaggerated response of the immune system (a hypersensitivity response) to the fungus Aspergillus (most commonly Aspergillus fumigatus). It occurs most often in patients with asthma or cystic fibrosis. Aspergillus spores are ubiquitous in soil and are commonly found in the sputum of healthy individuals. A. fumigatus is responsible for a spectrum of lung diseases known as aspergilloses. ABPA causes airway inflammation, leading to bronchiectasis-a condition marked by abnormal dilation of the airways. Left untreated, the immune system and fungal spores can damage sensitive lung tissues and lead to scarring. The exact criteria for the diagnosis of ABPA are not agreed upon. Chest X-rays and CT scans, raised blood levels of IgE and eosinophils, immunological tests for Aspergillus together with sputum staining and sputum cultures can be useful. Treatment consists of corticosteroids and antifungal medications. Almost all ...
Direct utilization of untreated oil palm trunk (OPT) for cellulases and xylanase production by Aspergillus fumigatus SK1 was conducted under solid-state fermentation (SSF). The highest activities of extracellular cellulases and xylanases were produced at 80% moisture level, initial pH 5.0, 1 × 108 spore/g (inoculum) with 125 μm of OPT as sole carbon source. The cellulases and xylanase activities obtained were 54.27, 3.36, 4.54 and 418.70 U/g substrates for endoglucanase (CMCase), exoglucanase (FPase), β-glucosidase and xylanase respectively. The crude cellulases and xylanase required acidic condition to retain their optimum activities (pH 4.0). Crude cellulases and xylanase were more stable at 40°C compared to their optimum activities conditions (60°C for FPase and 70°C for CMCase, β-glucosidase and xylanase). SDS-PAGE and zymogram analysis showed that Aspergillus fumigatus SK1 could secrete cellulases (endoglucanase, exoglucanase and β-glucosidase), xylanase and protease. Enzymatic ...
In vitro pharmacokinetic/pharmacodynamic data of liposomal amphotericin B (L-AMB) were compared with animal data from neutropenic and nonneutropenic models of azole-susceptible and azole-resistant invasive aspergillosis. L-AMB was equally effective. The in vitro fCmax (maximum concentration of free drug)/MIC ratio associated with 50% of maximal activity was 0.31 (0.29 to 0.33), similar to that in neutropenic but not nonneutropenic mice (0.11 [0.06 to 0.20]). Simulation analysis indicated that standard L-AMB doses (1 to 3 mg/kg) are adequate for nonneutropenic patients, but higher doses (7.5 to 10 mg/kg) may be required for neutropenic patients for Aspergillus fumigatus isolates with MICs of 0.5 to 1 mg/liter. ...
To gain insight into aberrant cytokine regulation in cystic fibrosis (CF), we compared the phenotypic manifestations of allergen challenge in gut-corrected CFTR-deficient mice with background-matched C57Bl6 (B6) mice. Aspergillus fumigatus (Af) antigen was used to mimic allergic bronchopulmonary aspergillosis, a peculiar hyper-IgE syndrome with a high prevalence in CF patients. CFTR-/-, C57BL/6 and FVB/NJ mice were sensitized with Af antigen by serial intraperitoneal injections. Control mice were mock sensitized with PBS. Challenges were performed by inhalation of Af antigen aerosol. After Af antigen challenge, histologic analysis showed goblet cell hyperplasia and lymphocytic infiltration in both strains. However, total serum IgE levels were markedly elevated in CF mice. Sensitized CF mice showed a five-fold greater IgE response to sensitization as compared with B6- and FVB-sensitized controls. Additional littermate controls to fully normalize for B6-FVB admixture in the strain background confirmed the
Invasive mycoses represent a major cause of morbidity and mortality in patients with malignancy or undergoing hematopoietic stem cell transplantation (HSCT). Recently it has been shown that the Toll-like receptor system could play an important role in the development of invasive aspergillosis in patients receiving chemotherapy or after HSCT, and there is evidence suggesting that the involvement of Toll-like receptors during Aspergillus fumigatus infection is influenced by the immunological status of the host. The upper and lower respiratory tracts represent the most frequent locations of A. fumigatus infection in all patient categories and age groups. Among echinocandins, caspofungin has been approved for salvage therapy in patients with invasive aspergillosis, since it has been demonstrated to be well-tolerated and with a 39% response in patients with refractory infections. However, in this study as in other salvage therapy trials, the need for salvage therapy was established in the presence of
This study investigated the dynamics of Aspergillus fumigatus azole-resistant phenotypes in two compost heaps with contrasting azole exposures: azole free and azole exposed. After heat shock, to which sexual but not asexual spores are highly resistant, the azole-free compost yielded 98% (49/50) wild-type and 2% (1/50) azole-resistant isolates, whereas the azole-containing compost yielded 9% (4/45) wild-type and 91% (41/45) resistant isolates. From the latter compost, 80% (36/45) of the isolates contained the TR46/Y121F/T289A genotype, 2% (1/45) harbored the TR46/Y121F/M172I/T289A/G448S genotype, and 9% (4/45) had a novel pan-triazole-resistant mutation (TR463/Y121F/M172I/T289A/G448S) with a triple 46-bp promoter repeat. Subsequent screening of a representative set of clinical A. fumigatus isolates showed that the novel TR463 mutant was already present in samples from three Dutch medical centers collected since 2012. Furthermore, a second new resistance mutation was found in this set that ...
An overview of Allergic Bronchopulmonary Aspergillosis (ABPA) symptoms, diagnosis, treatment & management written by experts in allergy, asthma & immunology.
Allergic bronchopulmonary aspergillosis (ABPA), a progressive fungal allergic lung disease, is a common complication of asthma or cystic fibrosis. Although ABPA has been recognized since the 1950s, recent research has underscored the importance of Th2 immune deviation and granulocyte activation in its pathogenesis. There is also strong evidence of widespread under-diagnosis due to the complexity and lack of standardization of diagnostic criteria. Treatment has long focused on downregulation of the inflammatory response with prolonged courses of oral glucocorticosteroids, but more recently concerns with steroid toxicity and availability of new treatment modalities has led to trials of oral azoles, inhaled amphotericin, pulse intravenous steroids, and subcutaneously-injected anti-IgE monoclonal antibody omalizumab, all of which show evidence of efficacy and reduced toxicity.
Chaudhary N, Staab JF, Marr KA. PLoS One 2010;5:e9036.. Aspergillus allergens are described as proteins that are recognized in patients with hypersensitivity syndromes such as allergic bronchopulmonary aspergillosis and Aspergillus-induced asthma. However, findings from this study indicate that (at least) some of these proteins are not only allergens, but are also capable of inducing a T-helper 1 (Th1) cytokine response in volunteers without a history of suspected or proven fungal infection or reported allergy or atopy. This indicates that these Asp f proteins are able to induce both protective (Th1) and non-protective (Th2) inflammation. Depending on the hosts status, Aspergillus fumigatus is able to cause several diseases ranging from allergic (e.g. Aspergillus-induced asthma and allergic bronchopulmonary aspergillosis) to invasive (e.g. pulmonary aspergillosis and disseminated disease). A fumigatus conidia enter the body via the air and, if they are not cleared by phagocytic cells, spores ...
Allergic Bronchopulmonary Aspergillosis - Learn about the causes, symptoms, diagnosis & treatment from the Merck Manuals - Medical Consumer Version.
A collection of disease information resources and questions answered by our Genetic and Rare Diseases Information Specialists for Allergic bronchopulmonary aspergillosis
TY - JOUR. T1 - Fatal mycotic encephalitis in a Northern bottlenose whale (Hyperoodon ampullatus) caused by Aspergillus fumigatus. AU - Foster, G. AU - Dagleish, Mark P. AU - Reid, RJ. AU - Barley, Jason. AU - Howie, F. PY - 2008/11/30. Y1 - 2008/11/30. M3 - Article. VL - 163. SP - 602. EP - 604. JO - Veterinary Record. JF - Veterinary Record. SN - 0042-4900. IS - 20. ER - ...
Overview: What every practitioner needs to know Are you sure your patient has allergic bronchopulmonary aspergillosis? What are the typical findings for this disease? Allergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity lung disease due to bronchial colonization by Aspergillus fumigatus that occurs in susceptible patients with asthma and cystic fibrosis (CF). ABPA affects approximately 1-2%…. ...
Overview: What every practitioner needs to know Are you sure your patient has allergic bronchopulmonary aspergillosis? What are the typical findings for this disease? Allergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity lung disease due to bronchial colonization by Aspergillus fumigatus that occurs in susceptible patients with asthma and cystic fibrosis (CF). ABPA affects approximately 1-2%…. ...
Al harboured the same genotype of multi-triazole resistant A. fumigatus, isolated on two different occasions which raises concern on the exposure of hospitalized patients to this resistant genotype. In this context it is pertinent to mention that previously multi-triazole resistant TR34/L98H A. KS 176 biological activity fumigatus isolates have been reported from patients attending the outpatient departments of VPCI who were never exposed to azoles [22]. In addition multi-triazole resistant A. fumigatus has also been isolated from admitted patients of VPCI. The presence of A. fumigatus resistant to medical triazoles poses a threat to immunocompromised patients as alternative therapy is limited. Snelders et al. reported that TR34/L98H isolates from clinical and environmental origins were cross resistant to five triazole DMIs fungicides, propiconazole, bromuconazole, tebuconazole, epoxiconazole and difenoconazole and thus supporting the hypothesis that exposure of A. fumigatus to azole fungicides ...
Minimal Diagnostic Criteria for ABPA-Central Bronchiectasis (ABPA-CB) Asthma Central bronchiectasis Immediate cutaneous reactivity to Aspergillus species or A. fumigatus Elevated total serum IgE (>417 kU/L) Elevated serum IgE-A.fumigatus and or IgG- A.fumigatus compared to sera from prick positive patients with asthma
Non-fumigatus Aspergillus species are the leading cause of Aspergillus infections in the tropical and subtropical regions of the world. In a prospective study between 2015 and 2016, a total of 150 bronchoalveolar (BAL) specimens was collected from patients suspected to pulmonary aspergillosis (PA) underlying immunodeficiencies in Mashhad, Northeastern Iran, located in the Middle East. All Aspergillus strains were phylogenetically identified at the species level by PCR-sequencing of partial β-tubulin gene. Overall, Aspergillus species were isolated from 20 specimens originating from 10 (50%) patients with cancer, 5 (25%) patients receiving corticosteroid therapy, 3 (15%) organ transplant recipients and 2 (10%) patients admitted to intensive care unit (ICU ...
To investigate azole resistance in clinical Aspergillus isolates, we conducted prospective multicenter international surveillance. A total of 3,788 Aspergillus isolates were screened in 22 centers from 19 countries. Azole-resistant A. fumigatus was more frequently found (3.2% prevalence) than previo …
Question - Have allergic aspergillosis. Would a flare up result in low blood oxygen level?. Ask a Doctor about diagnosis, treatment and medication for Allergic bronchopulmonary aspergillosis, Ask an OBGYN, Gynecologic Oncology
Many patients with cystic fibrosis (CF) cough up mucus or have throat cultures that grow a common fungus called Aspergillus. In patients with CF, aspergillus is not known to cause direct damage to the lungs, but some patients respond with an allergic reaction that causes them to wheeze, cough, or have difficulty breathing. This allergic reaction is called ABPA. Current treatment for ABPA includes high dose steroids and an anti-fungal medicine. Treatment with steroids may be problematic for some people due to its side effects on blood sugar levels and the bones. Steroids are medications that decrease inflammation, including prednisone, medrol, dexamethasone and others.. Ongoing research at UPMC on the study Mechanisms of Immune Tolerance in ABPA has studied people with CF and ABPA versus those patients with CF that just grow A. fumigatus (Af) in the sputum, but do not have ABPA. You may have participated in this study. This study has shown that people with CF with the fungus, Af, in their ...
Aspergillus fumigatus, an opportunistic pathogenic fungus, is the major reason for invasive aspergillosis and constitute also as an allergenic origin. Despite of the importance of the pathogenity of this fungus, only little is known about the background of its pathogenity. A. fumigatus produces a series of secondary metabolites which can be alone or together responsible for the pathogenity. For example, this fungus produces gliotoxin, an epipolythiodixopiperazine, which is in vitro characterised as a potent agent for cell death. For this reason it was speculated, that this mycotoxin might be involved in the pathogenesis of A. fumigatus. It is still necessary to know more about the genetic information and biosynthesis of further secondary metabolites, which might function as virulence factors. The biosynthetic gene cluster of gliotoxin has been identified in A. fumigatus. In the genome sequence of A. fumigatus Af293, an additional putative biosynthetic gene cluster containing three genes with ...
By Jennifer Doucet & Anushka Jayasuriya - June 23, 2017. Microbes, including bacteria and fungi, have many tools to evade a host cells defenses and antimicrobial therapies. One tool is the creation of protective barriers called biofilms, made of proteins, DNA, and sugar polymers, which spread across surfaces and impede immune system responses and antimicrobial treatments. In fact, biofilms can make microbes up to 1000 times more resistant to treatment. Despite their critical role in resistance to antimicrobials, there are no currently licensed therapies that target mature biofilms.. The bacteria Pseudomonas aeruginosa and the fungus Aspergillus fumigatus both form sugar-rich biofilms in host tissues during infection. They cause serious infections worldwide, especially in immunocompromised patients or those with chronic disease. Additionally, co-colonization by both P. aeruginosa and A. fumigatus has been observed in patients with lung disease such as cystic fibrosis.. As recently published in ...
The used ABPA treatment regimen restored forced expiratory volume in 1 second (FEV) values to pre-ABPA levels within three months (p
Learn about the causes, symptoms, diagnosis & treatment of Asthma and Related Disorders from the Professional Version of the Merck Manuals.
Cardiology news, research and treatment articles offering cardiology healthcare professionals cardiology information and resources to keep them informed.
Invasive aspergillosis is still a major fungal infection in immunocompromised patients. On the other hand, on time diagnosis of disease in the primary stages of infection is highly imperative for appropriate antifungal therapy and decreases the mortality rate in suspected groups (16). There are several approaches for detection and diagnosis of IA in clinical samples of patients. However, conventional methods, including direct examination and culture, are the gold standard to identify the causing agents, but there are some limitations, such as being time consuming, lack of proper sensitivity in early stages of the disease, as well as false negative results when the patients take the antifungal drugs (17).. Molecular diagnostic methods including real-time PCR as well as GM- EIA are more sensitive and specific and can approve an early IA diagnosis clinical outcome, when the other diagnostic methods are negative. Moreover, rapid results could be offered due to GM- EIA and real-time PCR assay ...
In recent years acquired azole resistance in Aspergillus fumigatus has been increasingly reported and a dominant mechanism of resistance (TR34/L98H) was found in clinical and environmental isolates. The aim of the present study was to investigate the prevalence of azole resistance in environmental A. fumigatus isolates collected in northern Italy. A. fumigatus grew from 29 of 47 soil samples analysed. Azole-resistant isolates were detected in 13% (6/47) of the soil samples and in 21% (6/29) of the soil samples containing A. fumigatus. High minimal inhibitory concentrations (MIC) of itraconazole (≥16 mg/L) and posaconazole (≥0.5 mg/L) were displayed by nine isolates from six different soil samples, namely apple orchard (1 sample), rose pot compost (2 samples), and cucurbit yields (3 samples). Seven isolates had a MIC=2 mg/L of voriconazole. Seven of nine itraconazole and posaconazole resistant isolates harboured the same TR34/L98H mutation of cyp51A. These findings, together with the occurrence of
Various saprotrophic microorganisms, especially filamentous fungi, can efficiently degrade lignocellulose that is one of the most abundant natural materials on earth. It consists of complex carbohydrates and aromatic polymers found in the plant cell wall and thus in plant debris. Aspergillus fumigatus Z5 was isolated from compost heaps and showed highly efficient plant biomass-degradation capability. The 29-million base-pair genome of Z5 was sequenced and 9540 protein-coding genes were predicted and annotated. Genome analysis revealed an impressive array of genes encoding cellulases, hemicellulases and pectinases involved in lignocellulosic biomass degradation. Transcriptional responses of A. fumigatus Z5 induced by sucrose, oat spelt xylan, Avicel PH-101 and rice straw were compared. There were 444, 1711 and 1386 significantly differently expressed genes in xylan, cellulose and rice straw, respectively, when compared to sucrose as a control condition. Combined analysis of the genomic and transcriptomic
Axel Brakhage. Director of the Leibniz-Institute for Natural Product Research and Infection Biology - Hans-Knoell-Institute (HKI). and. Professor and Chair of Microbiology / Molecular Biology. Friedrich-Schiller-University Jena. Department Molecular and Applied Microbiology. Research. Pathogenicity of Aspergillus fumigatus and secondary metabolism of Aspergillus nidulans. ...
Healthwise knowledgebase health facts. This records does no longer update the recommendation of a physician. Healthwise, incorporated, disclaims any guarantee or legal responsibility to your use of this data.. situations we treat nyu langone scientific middle. Nyu langone medical doctors and other professionals deal with heaps of situations, sicknesses, illnesses, and accidents. Learn extra.. Set definition of set by using the unfastened dictionary. Set (sĕt) n. Mythology variant of seth2. Set 1 (sĕt) v. Set, putting, sets v.Tr. 1. To put in a specified position or arrangement; region set a e-book on a table.. # diabetes causes yeast infection diabetic. ★ diabetes causes yeast infection ★ diabetic stuffed peppers the 3 step trick that reverses diabetes completely in as little as eleven days.[ diabetes reasons.. Aspergillus fumigatus and aspergillosis pubmed central. Aspergillus fumigatus is a saprophytic fungus that plays an critical position in recycling environmental carbon and nitrogen ...
Prolonged myelosuppression following CD19-directed CAR T-cell transfusion represents an important, yet underreported, adverse event. The resulting neutropenia and multifactorial immunosuppression can facilitate severe infectious complications. We describe the clinical course of a 59-year-old patient with relapsed/refractory DLBCL who received Axicabtagene-Ciloleucel (Axi-cel). The patient developed ASTCT grade I CRS and grade IV ICANS, necessitating admission to the neurological ICU and prolonged application of high-dose corticosteroids and other immunosuppressive agents. Importantly, neutropenia was profound (ANC | 100/μl), G-CSF-refractory, and prolonged, lasting more than 50 days. The patient developed severe septic shock 3 weeks after CAR transfusion while receiving anti-fungal prophylaxis with micafungin. His clinical status stabilized with broad anti-infective treatment and intensive supportive measures. An autologous stem cell backup was employed on day 46 to support hematopoietic recovery.
Species: Bovine, equine, canine and cats. Specimen: Depends on site of infection, e.g. foetal stomach content in abortion cases, swabs from nasal infections, tissue biopsies.. Container: Sterile container with tissue, fluid, swab. Collection protocol: Depends on sample type may involve removal of cotyledons or tissue biopsy, aspiration of fluid, nasal swab.. Special handling/shipping requirements: Dry swab samples are stable at room temperature. Other samples are to be transported and stored chilled. Do not freeze samples.. General information about the disease: Can cause site-specific mycotic disease e.g. mycotic abortion in cattle, guttural pouch mycosis in horses, nasal infections in dogs and cats. Infection is acquired from environmental sources, generally inhalation or ingestion. It is an opportunistic pathogen depending on impaired, overwhelmed or by-passed host defences to permit hyphal invasion of the tissues. Comparison with other related tests: PCR provides rapid detection and specific ...
Chitinase inhibitors have chemotherapeutic potential as fungicides, pesticides, and antiasthmatics. Argifin, a natural product cyclopentapeptide, competitively inhibits family 18 chitinases in the nanomolar to micromolar range and shows extensive substrate mimicry
The fungi Aspergillus flavus,[108] Aspergillus fumigatus,[108] Aspergillus niger,[108] Aspergillus parasiticus, Aspergillus ... tamarii, Aspergillus sulphureus, Aspergillus repens, Mucor hiemalis (not a human pathogen), Penicillium chrysogenum, ... Oven treatment killed conidia of A. fumigatus, A. flavus and A. niger, and did not degrade the active component of marijuana, ... Penicillium italicum and Rhizopus nigrans have been found in moldy cannabis.[107] Aspergillus mold species can infect the lungs ...
AF - Aspergillus fumigatus mutant C-2. *EC - Escherichia coli. *BT - Bacillus thuringiensis ...
"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae". Nature. 438 (7071): 1105-15. ... Aspergillus nidulans Strain:FGSC A4, model organism (2005[20]). *Aspergillus niger Strain:ATCC 1015 (DOE Joint Genome institute ... Aspergillus oryzae Strain:RIB40, industrial use (2005[22]). *Aspergillus terreus NIH 2624, statin producer and pathogen (2005, ... "Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus". PLoS Genet. 4 (4): e1000046. doi:10.1371/journal. ...
Opportunistic infection by molds[25] such as Penicillium marneffei and Aspergillus fumigatus is a common cause of illness and ... McCormick A, Loeffler J, Ebel F (November 2010). "Aspergillus fumigatus: contours of an opportunistic human pathogen". Cell. ... Light micrograph of the hyphae and spores of the human pathogen Aspergillus fumigatus ... an update on the pathogenesis of Aspergillus fumigatus infection". Br. J. Haematol. 150 (4): 406-17. doi:10.1111/j.1365- ...
"Aspergillus fumigatus: New Opportunities for Treatment of Multidrug-Resistant Fungal Disease". mBio. 8 (4): e01157-17. doi: ...
Aspergillus fumigatus (nepohlavné rozmnožovanie konídiami) Druh je heterothalický:. a) v prípade zhodnej časovej a priestorovej ... Dovičičová M. (2010): Štúdium potenciálne toxinogénnych druhov rodu Aspergillus a asociovaných teleomorf izolovaných zo zrna ... Rod Aspergillus (Pitt et Hocking, 1999) bol prvýkrát opísaný v roku 1729 talianskym kňazom a mykológom Pierom Antoniom Michelim ... Aspergillus nidulans (nepohlavné rozmnožovanie konídiami) Druh je homothalický: kolónie izolátov sú po kultivácii väčšinou ...
"Toll-like receptor 9-dependent immune activation by unmethylated CpG motifs in Aspergillus fumigatus DNA". Infect. Immun. 76 (5 ...
The fungus Aspergillus fumigatus effectively degrades plasticized PVC.[58] Phanerochaete chrysosporium has been grown on PVC in ... a mineral salt agar.[59] Phanerochaete chrysosporium, Lentinus tigrinus, Aspergillus niger, and Aspergillus sydowii can also ...
lycopersici and Aspergillus fumigatus. Kanini, Grammatiki S.; Katsifas, Efstathios A.; Savvides, Alexandros L.; Karagouni, ...
Marshall AC, Kidd SE, Lamont-Friedrich SJ, Arentz G, Hoffmann P, Coad BR, Bruning JB (March 2019). "Aspergillus fumigatus ...
"The biosynthesis of 4-hydroxycoumarin and dicoumarol by Aspergillus fumigatus Fresenius". The Biochemical Journal. 117 (2): 237 ...
Aspergillus fumigatus, the most common cause of fungal infection in the lungs of immune compromised patients often resulting in ... In Asia, Aspergillus oryzae is added to a pulp of soaked soya beans to make soy sauce, and is used to break down starch in rice ... Most famously, Neurospora crassa, several species of yeasts, and Aspergillus species are used in many genetics and cell biology ... Examples of ascomycetes that can cause infections in humans include Candida albicans, Aspergillus niger and several tens of ...
Heddergott, C.; Calvo, A. M.; Latgé, J. P. (2017-02-02). "The Volatome of Aspergillus fumigatus". Eukaryotic Cell. 13 (8): 1014 ...
Latgé JP, Chamilos G (December 2019). "Aspergillus fumigatus and Aspergillosis in 2019". Clinical Microbiology Reviews. 33 (1 ... Aspergillus is a fungus, commonly associated with respiratory infection. Candida albicans is a species of fungus that is ...
"Bioactive alkaloids from endophytic Aspergillus fumigatus". J Nat Prod. 72 (4): 753-5. doi:10.1021/np800700e. PMID 19256529. ... Hawas UW, El-Beih AA, El-Halawany AM (2012). "Bioactive anthraquinones from endophytic fungus Aspergillus versicolor isolated ... Fungi portal Bacillus isolates Biotechnology in pharmaceutical manufacturing Mycorrhiza Aspergillus oryzae, Saccharomyces ... two benzylazaphilones with an unprecedented carbon skeleton from the gorgonian-derived fungus Aspergillus sp". Bioorg Med Chem ...
Ge HM; Yu ZG; Zhang J; Wu JH; Tan RX (2009). "Bioactive alkaloids from endophytic Aspergillus fumigatus". J Nat Prod. 72 (4): ...
Rigbers O, Li SM (October 2008). "Ergot alkaloid biosynthesis in Aspergillus fumigatus. Overproduction and biochemical ... "An old yellow enzyme gene controls the branch point between Aspergillus fumigatus and Claviceps purpurea ergot alkaloid ... "Ergot cluster-encoded catalase is required for synthesis of chanoclavine-I in Aspergillus fumigatus". Current Genetics. 57 (3 ... Chanocalvine-I is then oxidized to chanoclavine-I aldehyde with NAD+ dependent enzyme EasD (FgaDH in A. fumigatus). ...
Rigbers O, Li SM (October 2008). "Ergot alkaloid biosynthesis in Aspergillus fumigatus. Overproduction and biochemical ...
Aspergillus fumigatus, is a heterothallic fungus. It is one of the most common Aspergillus species to cause disease in humans ... Examples of heterothallism are included for Saccharomyces cerevisiae, Aspergillus fumigatus, Aspergillus flavus, Penicillium ... "Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus fumigatus". Nature. 457 (7228): 471-4. Bibcode: ... "Low genetic variation and no detectable population structure in aspergillus fumigatus compared to closely related Neosartorya ...
Ge HM, Yu ZG, Zhang J, Wu JH, Tan RX (2009). "Bioactive alkaloids from endophytic Aspergillus fumigatus". J Nat Prod. 72 (4): ... 14-Norpseurotin A is an alkaloid and a bio-active metabolite of Aspergillus, featuring an oxa-spiro-lactam core. Pseurotin A ... "Antiparasitic and anticancer constituents of the endophytic fungus Aspergillus sp. strain F1544". Nat Prod Commun. 7 (2): 165-8 ...
In Aspergillus fumigatus, a causative agent of aspergillosis, galactosaminogalactan is required for adherence to host tissue, ... Overlapping and distinct roles of Aspergillus fumigatus UDP-glucose 4-epimerases in galactose metabolism and the synthesis of ... 2014). A polysaccharide virulence factor from Aspergillus fumigatus elicits anti-inflammatory effects through induction of ... 2011). Galactosaminogalactan, a new immunosuppressive polysaccharide of Aspergillus fumigatus. In PLoS Pathog, pp. e1002372 ...
Die fadenpilze, aspergillus flavus, niger u. fumigatus; eurotium repens (u. aspergillus glaucus) und ihre beziehungen zur ... otomycosis aspergillina, 1883 - The filamentous fungi, Aspergillus flavus, niger and fumigatus; Eurotium repens (Aspergillus ...
In Aspergillus fumigatus, the enzymes needed for gliotoxin biosynthesis are encoded in 13 genes within the gli gene cluster. ... The compound is produced by human pathogens such as Aspergillus fumigatus, and also by species of Trichoderma and Penicillium. ... Gliotoxin is hypothesized to be an important virulence factor in Aspergillus fumigatus. Experiments have demonstrated that ... gliotoxin is isolated in the highest concentrations from Aspergillus fumigatus in comparison to other Aspergillus species. This ...
Rarely, Aspergillus fumigatus and mucormycosis cause CST. Aseptic cavernous sinus thrombosis is much less common and is usually ...
For example, scientists detected that Aspergillus fumigatus var. fumigatus, a fungus which triggers allergic reactions in the ...
The fungus Aspergillus fumigatus effectively degrades plasticized PVC. Phanerochaete chrysosporium has been grown on PVC in a ...
Opportunistic infection by molds such as Talaromyces marneffei and Aspergillus fumigatus is a common cause of illness and death ... McCormick A, Loeffler J, Ebel F (November 2010). "Aspergillus fumigatus: contours of an opportunistic human pathogen". Cellular ... an update on the pathogenesis of Aspergillus fumigatus infection". British Journal of Haematology. 150 (4): 406-17. doi:10.1111 ... Molds that are often found on meat and poultry include members of the genera Alternaria, Aspergillus, Botrytis, Cladosporium, ...
Beauvais A, Latgé JP (February 2001). "Membrane and cell wall targets in Aspergillus fumigatus". Drug Resistance Updates. 4 (1 ... and Aspergillus spp. show a "paradoxic effect", i.e., they are susceptible to low concentrations but resistant to high ... Echinocandins are fungistatic against some molds (Aspergillus, but not Fusarium and Rhizopus), and modestly or minimally active ... They have fungistatic activity against Aspergillus species. and fungicidal activity against most Candida spp., including ...
... to the fungus Aspergillus (most commonly Aspergillus fumigatus). It occurs most often in people with asthma or cystic fibrosis ... Aspergillus spores are ubiquitous in soil and are commonly found in the sputum of healthy individuals. A. fumigatus is ... The first stage involves exposing the skin to Aspergillus fumigatus antigens; an immediate reaction is hallmark of ABPA. The ... Hohl, Tobias M.; Feldmesser, Marta (November 2007). "Aspergillus fumigatus: Principles of Pathogenesis and Host Defense". ...
The fungus Aspergillus fumigatus effectively degrades plasticized PVC. Phanerochaete chrysosporium was grown on PVC in a ... Phanerochaete chrysosporium, Lentinus tigrinus, Aspergillus niger, and Aspergillus sydowii can effectively degrade PVC. ...
Aspergillus niger fungus liquor awamori [2] Aspergillus fumigatus fungus chocolate [1] Aspergillus oryzae fungus soy miso [2][5 ...
... როგორიცაა Aspergillus fumigatus, Scedosporium apiospermum, Aspergillus terreus) და/ან საფუარები (როგორიცაა Candida albicans); ... მათ შორისაა ბრონქოპულმონარული ასპერგილოზი, როდესაც ორგანიზმის საპასუხო რეაქცია სოკო Aspergillus fumigatus-ის მიმართ იწვევს ... სხვა ფილამენტური სოკოებიდან იშვიათად გვხვდება Aspergillus flavus, Aspergillus nidulans, Exophiala dermatitidis და Scedosporium ...
Aspergillus fumigatus, Candida albicans hsp60, as peptide transporter and adjuvant for antigen presentation. - ...
... and the fungi Aspergillus fumigatus, Candida albicans and Cryptococcus neoformans.[44]. Viruses may also undergo sexual ...
Saccharomyces cerevisiae and Aspergillus fumigatus.[24] It can also inhibit the growth of Helicobacter pylori.[25] So important ...
... ki jo povzroča preobčutljivost na glive iz rodu Aspergillus (in sicer Aspergillus fumigatus).[1] Spore glive Aspergillus bolnik ... povišani specifični IgE in IgG za Aspergilus fumigatus, s pomočjo visokoločljivostne računalniške tomografije vidne centralne ... prisotnost serumskih precipitinov za Aspergilus fumigatus, pozitivni kožni testi za aspergilusni antigen, povišani celokupni ...
... u Aspergillus fumigatus). ...
... despite several contacts with Aspergillus fumigatus conidia (unlabeled, white/clear) in a 3-D collagen matrix. Imaging time was ...
... that affects the airways of the lungs which is caused by an allergic hypersensitivity to the fungus Aspergillus fumigatus. ...
... o Cryptococcus neoformans e o Aspergillus fumigatus causan a maioría destas infeccións e presentan con frecuencia reistencia ...
Chronic infections often occur in those with immunodeficiency and can include a fungal infection by Aspergillus fumigatus that ...
Aspergillus foetidus. Aspergillus fumigatus. Aspergillus glaucus. Aspergillus lentulus. Aspergillus nidulans. Aspergillus niger ... Aspergillus restrictus. Aspergillus sojae. Aspergillus sydowii. Aspergillus tamari. Aspergillus terreus. Aspergillus ustus. ... Aspergillus alliaceus. Aspergillus caesiellus. Aspergillus caespitosus. Aspergillus candidus. Aspergillus carneus. Aspergillus ... Aspergillus deflectus. Aspergillus egyptiacus. Aspergillus fischerianus. Aspergillus flavus. ...
... is a fungal disease caused by Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger. It causes mummification of the ...
The genome sequences of three Aspergillus species-Aspergillus fumigatus, Aspergillus nidulans, and Aspergillus oryzae-were ... Aspergillus fumigatus is a species of fungus in the genus Aspergillus, and is one of the most common Aspergillus species to ... Aspergillus fumigatus grown on certain building materials can produce genotoxic and cytotoxic mycotoxins, such as gliotoxin.[7] ... Aspergillus fumigatus can survive on a variety of different nitrogen sources, and the assimilation of nitrogen is of clinical ...
... is a long-term aspergillus infection of the lung and Aspergillus fumigatus is almost always the species responsible for this ... It is very rarely caused by Aspergillus flavus, Aspergillus oryzae, Aspergillus terreus or Aspergillus nidulans. Patients with ... Almost all aspergillomas are caused by Aspergillus fumigatus. In diabetic patients it may be caused by Aspergillus niger. ... Serum precipitins for aspergillus (blood test to detect antibodies to aspergillus) ...
Chronic infections often occur in those with immunodeficiency and can include a fungal infection by Aspergillus fumigatus that ... "The human lung and Aspergillus: You are what you breathe in?". Medical Mycology. 57 (Supplement_2): S145-S154. doi:10.1093/mmy ...
Pseudomonas lemoigne, Comamonas sp. Acidovorax faecalis, Aspergillus fumigatus and Variovorax paradoxus are soil microbes ... and a thermophilic strain of Aspergillus sp.[12] ...
Aspergillus fumigatus da aspergilosi askoren eragilea, baina Aspergillus flavus eta Aspergillus nigerren garrantzia ere ... Aspergillus onddoaren aurrean sortzen den alergia mota bat da. Asma, errinitisa, sukarra, eta eztula dira sintoma nagusiak. ... Horregatik aipatzen da Aspergillus onddo oportunista bat dela. Aspergilosi guztiek arnas aparatua eta birikak kaltetzen dituzte ... Aspergillusen esporak inhalatzerakoan harrapatu ohi dira aspergilosiak. Onddoa nonahikoa denez (lur-zoruan, hautsean, ...
The fungus Aspergillus fumigatus effectively degrades plasticized PVC.[38] Phanerochaete chrysosporium was grown on PVC in a ... mineral salt agar.[39] Phanerochaete chrysosporium, Lentinus tigrinus, Aspergillus niger, and Aspergillus sydowii can ...
December 2005). "Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae". Nature. 438 ( ... Aspergillus oryzae, known in English as koji (Japanese: 麹, Hepburn: kōji), is a filamentous fungus (a mold) used in Chinese and ... Fujita, Chieko, Tokyo Foundation Koji, an Aspergillus *^ Shurtleff, W.; Aoyagi, A. History of Koji - Grains and/or Soybeans ... Aspergillus oryzae genome from the Database of Genomes Analysed at NITE (DOGAN) ...
The genome sequences of three Aspergillus species-Aspergillus fumigatus, Aspergillus nidulans, and Aspergillus oryzae-were ... Aspergillus fumigatus is a species of fungus in the genus Aspergillus, and is one of the most common Aspergillus species to ... Aspergillus fumigatus grown on certain building materials can produce genotoxic and cytotoxic mycotoxins, such as gliotoxin.[7] ... Aspergillus fumigatus can survive on a variety of different nitrogen sources, and the assimilation of nitrogen is of clinical ...
Aspergillus fumigatus is a pathogenic filamentous fungus and is responsible for more infections worldwide than any other mould ... May 2008). "Small ncRNA transcriptome analysis from Aspergillus fumigatus suggests a novel mechanism for regulation of protein ... The table below summarised the type, genome location and corresponding target sites of the ncRNAs identified in A. fumigatus. ... Comparative genomics showed that the snoRNAs present within A. fumigatus are conserved across other strains of fungus. This ...
Aspergillus fumigatus. A. fumigatus causes more infections worldwide than any other mould. Four percent of all patients dying ... Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus.. Nierman WC, Pain A, Anderson MJ, ... Aspergillus fumigatus . This involved preparing a bacterial artificial chromosome (BAC) library, to fingerprint 3000 BAC clones ... The Sanger Institute was then funded to carry our a whole genome shotgun of A. fumigatus in collaboration with The Institute of ...
Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
Large-scale genome comparisons have shown that no gene sets are shared exclusively by both Aspergillus fumigatus and any other ... Aspergillus fumigatus: saprophyte or pathogen? Curr Opin Microbiol. 2005 Aug;8(4):385-92. doi: 10.1016/j.mib.2005.06.017. ... Large-scale genome comparisons have shown that no gene sets are shared exclusively by both Aspergillus fumigatus and any other ... fumigatus genome. In addition, the proteome of this fungus contains numerous efflux pumps, including ,100 major facilitators ...
... fumigatus has been associated with each and every type of health problems linked to environmental moulds: irritation and ... Aspergillus fumigatus Basics Laboratory Metabolites Adverse health reactions Specific settings Diagnostic Bibliography Basics A ... Aspergillus fumigatus is however the most important species in Aspergillus genus causing health problems. This species has been ... Health risks associated with exposure to Aspergillus fumigatus are well established. Most important, A. fumigatus is the main ...
Aspergillus fumigatus ATCC ® 28215™ Designation: AF4 Application: Biomedical Research and Development Material Emerging ... Aspergillus fumigatus Fresenius (ATCC® 28215™) Strain Designations: AF4 / Product Format: freeze-dried ...
Aspergillus fumigatus ATCC ® 1022™ Designation: NRRL 163 [118, CBS 133.61, IMI 16152, LSHB Ac71, NCTC 982, QM 1981, WB 163] ... Aspergillus fumigatus Fresenius (ATCC® 1022D-2™) Add to freeze-dried Total DNA: Approximately 2 µg in 1X Tris buffer. OD260/OD ... Aspergillus fumigatus Fresenius (ATCC® 1022™) Strain Designations: NRRL 163 [118, CBS 133.61, IMI 16152, LSHB Ac71, NCTC 982, ... Chemotypic and genotypic diversity in the ergot alkaloid pathway of Aspergillus fumigatus. Mycologia 104: 804-812, 2012. PubMed ...
Afut1, a retrotransposon-like element from Aspergillus fumigatus.. Neuveglise C1, Sarfati J, Latge JP, Paris S. ... A repeated DNA sequence used for epidemiological studies of the human opportunistic pathogen Aspergillus fumigatus has been ... This is the first report of a transposable element in A.fumigatus. Afut1 is a defective element: the putative coding domains ... Laboratoire des Aspergillus, Institut Pasteur, Paris, France.. Abstract. ...
... fumigatus for azole resistance is to use an agar plate assay that includes antifungal drugs in the agar. Growth on the agar ... One way to screen isolates of Aspergillus fumigatus for azole resistance is to use an agar plate assay that includes antifungal ... This procedure will be used for screening Aspergillus fumigatus isolates for azole antifungal resistance using a 4-quadrant ... The environmental mold Aspergillus fumigatus is the primary cause of invasive aspergillosis. In patients with high-risk ...
Aspergillus fumigatus ATCC ® MYA-3626™ Designation: T33439 Application: Antifungal susceptibility testing Ref Ref Emerging ... Aspergillus fumigatus Fresenius (ATCC® MYA-3626™) Strain Designations: T33439 / Product Format: frozen ...
Neosartorya udagawae (Aspergillus udagawae), an Emerging Agent of Aspergillosis: How Different Is It from Aspergillus fumigatus ... Critical Stages of Extracting DNA from Aspergillus fumigatus in Whole-Blood Specimens P. Lewis White, Michael D. Perry, Juergen ... Detection of Aspergillus fumigatus in a Rat Model of Invasive Pulmonary Aspergillosis by Real-Time Nucleic Acid Sequence-Based ... Culture-Based Methods and Molecular Tools for Azole-Resistant Aspergillus fumigatus Detection in a Belgian University Hospital ...
Testing Status of Aspergillus fumigatus mold 08022. CASRN: ASPERGILLUS. Short-Term Toxicity. *13 weeks (Inhalation) (C08022) ... Subchronic Toxicity of Aspergillus fumigatus in B6C3F1/N Mice. *Citation: Croston TL, Nayak AP, Lemons AR, Goldsmith WT, Gu JK ... Influence of Aspergillus fumigatus conidia viability on murine pulmonary microRNA and mRNA expression following subchronic ... Dose: 0 (Air), Particle Control (1 x 10^5 Heat Killed Spores), 1 x 10^5 A. Fumigatus spores; 10 Male/10 Female. ...
... a mycotoxin from the fungus Aspergillus fumigatus. The researchers present their findings in the current issue of the ... The fungus Aspergillus fumigatus occurs virtually everywhere on Earth, as a dark grey, wrinkled cushion on damp walls or in ... Researchers find how Aspergillus fumigatus knocks out immune defenses. *Download PDF Copy ... This does not happen if the pathogen Aspergillus fumigatus is involved. As the Jena scientists were able to show, gliotoxin ...
A total of 3,788 Aspergillus isolates were screened in 22 centers from 19 countries. Azole-resistant A. fumigatus was more ... To investigate azole resistance in clinical Aspergillus isolates, we conducted prospective multicenter international ... Prospective multicenter international surveillance of azole resistance in Aspergillus fumigatus Emerg Infect Dis. 2015 Jun;21(6 ... A total of 3,788 Aspergillus isolates were screened in 22 centers from 19 countries. Azole-resistant A. fumigatus was more ...
Thermotolerance and virulence of Aspergillus fumigatus: role of the fungal nucleolus.. Bhabhra R1, Askew DS. ... As a thermotolerant fungus, Aspergillus fumigatus is capable of growth at temperatures that approach the upper limit for all ... fumigatus. Future work in this area will help determine how rapid growth is accomplished at elevated temperature and may offer ...
A. Rivera, T. Hohl, and E. G. Pamer, "Immune responses to Aspergillus fumigatus infections," Biology of Blood and Marrow ... Elastase Activity in Aspergillus fumigatus Can Arise by Random, Spontaneous Mutations. Sergio Álvarez-Pérez,1 Jose L. Blanco,1 ... J.-P. Latgé, "The pathobiology of Aspergillus fumigatus," Trends in Microbiology, vol. 9, no. 8, pp. 382-389, 2001. View at ... J.-P. Latgé, "Aspergillus fumigatus and Aspergillosis," Clinical Microbiology Reviews, vol. 12, no. 2, pp. 310-350, 1999. View ...
El fumigatus fungoso del aspergillus ocurre virtualmente por todas partes en la tierra, como amortiguador gris oscuro, arrugado ... Los investigadores encuentran cómo el fumigatus del aspergillus elimina defensas inmunes. *Download PDF Copy ... Esto no suceso si el fumigatus del aspergillus el patógeno está implicado. Pues los científicos de Jena podían mostrar, el ... una micotoxina del fumigatus fungoso del aspergillus. Los investigadores presentan sus conclusión en la aplicación actual la ...
Aspergillus niger (ASPNG), Aspergillus flavus (ASPFL), Aspergillus polyvalent mix (M1), Aspergillus fumagatus specific IgE (RM3 ... Immunodiffusion (Precipitin Aspergillus Fumigatus #6 IgG). Related Tests. Allergic Bronchopulmonary Aspergillosis: ... Immunoglobulin E level (IGE), Aspergillus fumagatus #1 (ASPF1), ...
How Aspergillus allergens (Asp f proteins) participate in the development of allergic sensitization is unknown. Methodology/ ... Background Aspergillus fumigatus is associated with both invasive and allergic pulmonary diseases, in different hosts. The ... early responses may allow for development of novel diagnostics and therapeutics for both invasive and allergic Aspergillus ... Aspergillus fumigatus Is the Subject Area "Aspergillus fumigatus" applicable to this article? Yes. No. ...
Aspergillus fumigatus strains resistant to all triazole antifungal medications are emerging in the United States in patients ... Aspergillus fumigatus strains resistant to all triazole antifungal medications are emerging in the United States in patients ... First detection of TR34 L98H and TR46 Y121F T289A Cyp51 mutations in Aspergillus fumigatus isolates in the United States. J ... First detection of TR34 L98H and TR46 Y121F T289A Cyp51 mutations in Aspergillus fumigatus isolates in the United States. J ...
Aspergillus fumigatus / chemistry, metabolism*. Carbohydrate Sequence. Coenzyme A / metabolism. Ethanolamines / metabolism. ... Aspergillus fumigatus, a filamentous fungus, produces GPI-anchored molecules, some of them being essential in the construction ... Unité des Aspergillus, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France. [email protected] ... This latter series of GPI intermediates identified in the A. fumigatus cell-free system indicates that GPI biosynthesis in this ...
We propose that A. fumigatus acquisition of LoxB (found in few fungi) enhances germination rates in polyunsaturated fatty acid- ... We propose that A. fumigatus acquisition of LoxB (found in few fungi) enhances germination rates in polyunsaturated fatty acid- ... The opportunistic human pathogen Aspergillus fumigatus initiates invasive growth through a programmed germination process that ... The opportunistic human pathogen Aspergillus fumigatus initiates invasive growth through a programmed germination process that ...
For one of the major fungal pathogens, the environmental mold Aspergillus fumigatus, an interesting and relevant morphotype ... Resistance of A. fumigatus to antifungal treatment is influenced by several factors, among them biofilm formation (Seidler et ... Such hyphal communities were first described for A. fumigatus in 2007 (Mowat et al., 2007) and are nowadays recognized to be ... fumigatus biofilms are also formed in vivo during invasive aspergillosis or aspergilloma and have been characterized... ...
Os conhecimentos sobre a regulação dos níveis de cálcio e manganês no Aspergillus fumigatus são bastante limitados, sendo que a ... Os conhecimentos sobre a regulação dos níveis de cálcio e manganês no Aspergillus fumigatus são bastante limitados, sendo que a ... The knowledge about the regulation of Aspergillus fumigatus calcium and manganese levels are very limited, while these ions ... fumigatus (pALB1). Após indução da expressão, a construção de RNA dupla fita para RNAi silenciou tanto o gene alb1 isoladamente ...
Browse , Home / Archive by tags Aspergillus fumigatus Biofilms - the eradication has begun. Research Biofilms, the slimy, ...
Please note: The Aspergillus Website does not accept advertisments. , all content is published on merit. BB.com. Site Sponsors ... Aspergillus Website Newsletters. Our most recent newsletters (since August 2018) can be found here. ... Aspergillus fumigatus (click for details) Aspergillus fumigatus (click for details) Aspergillus fumigatus Fresenius (click for ...
  • [12] Additionally, A. fumigatus can cause chronic pulmonary infections, allergic bronchopulmonary aspergillosis , or allergic disease in immunocompetent hosts. (wikipedia.org)
  • Immunosuppressed individuals are susceptible to invasive A. fumigatus infection, which most commonly manifests as invasive pulmonary aspergillosis. (wikipedia.org)
  • The environmental mold Aspergillus fumigatus is the primary cause of invasive aspergillosis. (cdc.gov)
  • Neosartorya udagawae ( Aspergillus udagawae ), an Emerging Agent of Aspergillosis: How Different Is It from Aspergillus fumigatus ? (asm.org)
  • Azole-resistant A. fumigatus was more frequently found (3.2% prevalence) than previously acknowledged, causing resistant invasive and noninvasive aspergillosis and severely compromising clinical use of azoles. (nih.gov)
  • M. H. Kothary, T. Chase Jr., and J. D. MacMillan, "Correlation of elastase production by some strains of Aspergillus fumigatus with ability to cause pulmonary invasive aspergillosis in mice," Infection and Immunity , vol. 43, no. 1, pp. 320-325, 1984. (hindawi.com)
  • In this study we present antifungal susceptibility profile of A. fumigatus isolates recovered from lungs of birds with and without aspergillosis. (scielo.br)
  • Aspergillus belonging to section Fumigati is the main causative agent of aspergillosis in birds, it is commonly found in bed, soil and decomposing organic matter or in feed. (scielo.br)
  • Aspergillus species, especially Aspergillus fumigatus , is the most common cause of aspergillosis, which is the second leading cause of death after cryptococcosis in patients suffering from fungal infections [ 1 ]. (hindawi.com)
  • Despite the current use of Platelia ™ Aspergillus as a diagnostic test, the early diagnosis of invasive aspergillosis remains a major challenge in improving the prognosis of the disease. (mdpi.com)
  • Antigenic proteins expressed in the first steps of A. fumigatus germination occurring in a human host were revealed using 2-D Western immunoblots with the serum of patients who had previously been classified as probable and proven for invasive aspergillosis. (mdpi.com)
  • To our knowledge, this is the first report describing specific antigenic proteins of A. fumigatus germlings that are recognized by sera of patients with confirmed invasive aspergillosis who were from two separate hospital units. (mdpi.com)
  • Invasive aspergillosis (IA), often caused by Aspergillus fumigatus, is an important cause of death of immunocompromised patients. (hu-berlin.de)
  • Several DNA-extraction methods and PCR assays are available for detecting Aspergillus fumigatus DNA in bronchoalveolar lavage (BAL) samples of patients with invasive aspergillosis. (hu-berlin.de)
  • Aspergillus fumigatus is the main causative agent of aspergillosis. (springer.com)
  • Asexual spores of A. fumigatus are responsible for initiation of aspergillosis. (springer.com)
  • Dagenais TR, Keller NP (2009) Pathogenesis of Aspergillus fumigatus in Invasive Aspergillosis. (springer.com)
  • Aspergillosis associated with azole-resistant Aspergillus fumigatus has a mortality rate that can approach 90% in certain patient populations. (asm.org)
  • Our results demonstrate that ibrutinib-exposed neutrophils develop significant functional defects that impair their response against Aspergillus fumigatus, providing a plausible explanation for the emergence of invasive aspergillosis in ibrutinib-treated patients. (haematologica.org)
  • The species A. fumigatus was well-described and illustrated in the 1850s by Fresenius, working with lung material from birds dying of aspergillosis. (phadia.com)
  • Aspergillus fumigatus is an opportunistic fungus causing allergic and invasive aspergillosis in humans and animals. (phadia.com)
  • Bioluminescent Aspergillus fumigatus, a new tool for drug efficiency testing and in vivo monitoring of invasive aspergillosis. (biomedsearch.com)
  • Aspergillus fumigatus is the main cause of invasive aspergillosis in immunocompromised patients, and only a limited number of drugs for treatment are available. (biomedsearch.com)
  • Exposure to conidia of Aspergillus fumigatus was described as a causative factor of a number of the respiratory system diseases, including asthma, chronic eosinophilic pneumonia, hypersensitivity pneumonitis and bronchopulmonary aspergillosis. (aaem.pl)
  • In vivo data demonstrate that inactivated DapA combined with activated DapB yields an A. fumigatus mutant that is easily treatable with azoles in an immunocompromised mouse model of invasive pulmonary aspergillosis. (asm.org)
  • Invasive aspergillosis (IA) due to Aspergillus fumigatus is a major cause of mortality in immunocompromised patients. (jcvi.org)
  • In contrast to hyphae, we found no role for neutrophil calprotectin in uptake or killing of intracellular A. fumigatus conidia either in vitro or in a murine model of pulmonary aspergillosis. (jimmunol.org)
  • Diseases caused by the genus Aspergillus are named aspergillosis. (uwlax.edu)
  • http://www.doctorfungus.org/thefungi/aspergillus_fumigatus.htm , http://www.cdc.gov/nczved/dfbmd/disease_listing/aspergillosis_gi.html , or http://www.aspergillus.org.uk/indexhome.htm?languages/index.php~main . (uwlax.edu)
  • Abstract We evaluated extended-interval dosing of the investigational echinocandin rezafungin (1, 4, and 16 mg/kg on days 1, 4, and 7 post-inoculation) for the treatment of disseminated invasive aspergillosis caused by azole resistant Aspergillus fumigatus Survival was significantly improved in mice treated with each dose of rezafungin and supra-therapeutic posaconazole (20 mg/kg twice daily). (medworm.com)
  • The relevance of studies aimed at understanding host immune response against Aspergillus fumigatus takes on much significance given that all patients with invasive aspergillosis are invariably immunocompromised. (eur.nl)
  • A. fumigatus can cause invasive pulmonary aspergillosis, aspergilloma, immunoglobulin E-mediated allergic rhinitis and asthma, hypersensitivity pneumonitis, chronic necrotizing pneumonia, and allergic bronchopulmonary aspergillosis (ABPA). (asm.org)
  • Aspergillus fumigatus is a ubiquitous opportunistic fungal pathogen capable of causing invasive aspergillosis, a globally distributed disease with a mortality rate of up to 90% in high-risk populations. (ru.nl)
  • In allergic and immunocompromised individuals A. fumigatus can cause a wide spectrum of diseases, including allergic bronchopulmonary aspergillosis, aspergilloma and invasive aspergillosis. (biomedcentral.com)
  • Latgé, J. P. (1999) Aspergillus fumigatus and aspergillosis. (miltenyibiotec.com)
  • The assay was tested on (i) 2 A. lentulus and A. felis strains obtained from biopsy proven invasive aspergillosis and (ii) control A. fumigatus (n=3), A. lentulus (n=6) and A. felis species complex (n=12) strains. (eur.nl)
  • Aspergillosis ( Aspergillus spp. (biomedcentral.com)
  • Aspergillus fumigatus is a species of fungus in the genus Aspergillus , and is one of the most common Aspergillus species to cause disease in individuals with an immunodeficiency . (wikipedia.org)
  • Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. (sanger.ac.uk)
  • By contrast, and in agreement with the environmental occurrence of this fungus in decaying vegetation, the enzymatic machinery required by a fungus to colonize plant substrates has been found in the A. fumigatus genome. (nih.gov)
  • Aspergillus fumigatus is a pathogenic filamentous fungus and is responsible for more infections worldwide than any other mould. (wikipedia.org)
  • Comparative genomics showed that the snoRNAs present within A. fumigatus are conserved across other strains of fungus. (wikipedia.org)
  • Research team from the University of Jena (Germany) clarifies the mechanism of gliotoxin, a mycotoxin from the fungus Aspergillus fumigatus. (news-medical.net)
  • The fungus Aspergillus fumigatus occurs virtually everywhere on Earth, as a dark grey, wrinkled cushion on damp walls or in microscopically small spores that blow through the air and cling to wallpaper, mattresses and floors. (news-medical.net)
  • As a thermotolerant fungus, Aspergillus fumigatus is capable of growth at temperatures that approach the upper limit for all eukaryotes, suggesting that the organism has evolved unique mechanisms of stress resistance that may be relevant to its ability to adapt to the stress of growth in the host. (nih.gov)
  • Aspergillus fumigatus, a filamentous fungus, produces GPI-anchored molecules, some of them being essential in the construction of the cell wall. (biomedsearch.com)
  • This latter series of GPI intermediates identified in the A. fumigatus cell-free system indicates that GPI biosynthesis in this filamentous fungus is similar to the mammalian or yeast systems. (biomedsearch.com)
  • Two new compounds, fumitremorgin 12-methoxy-13-[5'-hydroxy-2'-(1''-hydroxy-3''-methoxy-5''-methylbenzoyl)-3'-methoxy]benzoic acid methyl ester (fumitremorgin D, 1 ) and 4,8,10,14-tetramethyl-6-acetoxy-14-[16-acetoxy-19-(20,21-dimethyl)-18-ene]-phenanthrene-1-ene-3,7-dione ( 2 ) were isolated from the cultured endophytic isolated fungus Aspergillus fumigatus , together with fourteen known compounds. (mdpi.com)
  • Zhao, C. An Alkaloid and a Steroid from the Endophytic Fungus Aspergillus fumigatus . (mdpi.com)
  • Liang Z, Zhang T, Zhang X, Zhang J, Zhao C. An Alkaloid and a Steroid from the Endophytic Fungus Aspergillus fumigatus . (mdpi.com)
  • Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus. (jcvi.org)
  • False colour scanning electron micrograph (SEM) of the spore-bearing bodies of the fungus Aspergillus fumigatus. (sciencephoto.com)
  • Aspergillus fumigatus , a filamentous fungus producing bluish-green conidia, is an important opportunistic pathogen that primarily affects immunocompromised patients. (asm.org)
  • SPERGILLUS fumigatus is a ubiquitous filamentous fungus of human environments and an opportunist pathogen (for review see Latgé 1999 , 2001 ). (genetics.org)
  • Aspergillus fumigatus is a ubiquitous opportunistic fungus. (springer.com)
  • Aspergillus fumigatus , a common airborne fungus and opportunistic human pathogen, and several fungi in the relatively distant taxon Clavicipitaceae (clavicipitaceous fungi) produce different sets of ergot alkaloids. (asm.org)
  • The rather distantly related imperfect fungus Aspergillus fumigatus , a common saprophyte and opportunistic human pathogen with close relatives in the order Eurotiales , produces ergot alkaloids in broth culture ( 4 , 23 ) and in association with its airborne conidia ( 13 ). (asm.org)
  • The images can be read by the appearance of an aspergilloma, a tumor made of Aspergillus fungus. (uwlax.edu)
  • The conidia of Aspergillus fumigatus ( A. fumigatus ), a common fungus, are ~2-3 µm in size and easily spread by wind ( 1 ). (spandidos-publications.com)
  • Objective: To report the cases of 6 patients with fungus ball caused by Aspergillus fumigatus (aspergilloma) in the pleural cavity. (ufrgs.br)
  • The saprophytic fungus Aspergillus fumigatus is a serious health hazard in hospitals ( Latge, 1999 , 2001 ). (rupress.org)
  • Aspergillus fumigatus (A. fumigatus) is a ubiquitous fungus which reproduces asexually by releasing abundant airborne conidia (spores), which are easily respirable. (biomedcentral.com)
  • Chamilos, Georgios 2017-09-08 00:00:00 More than 90 of the cell wall of the filamentous fungus Aspergillus fumigatus comprises polysaccharides. (deepdyve.com)
  • Influence of Aspergillus fumigatus conidia viability on murine pulmonary microRNA and mRNA expression following subchronic inhalation exposure. (nih.gov)
  • A. fumigatus is environmentally omnipresent and its airborne conidia are constantly inhaled to become eliminated by the functional host immune system. (frontiersin.org)
  • Inhalation delivery of A. fumigatus conidia increased the airway eosinophilia and bronchial hyper-responsiveness in ovalbumin-sensitized, challenged rats. (sigmaaldrich.com)
  • A. fumigatus conidia are more efficiently internalized by these cells and germination is delayed when compared to A. niger . (springer.com)
  • Amin S, Thywissen A, Heinekamp T, Saluz HP, Brakhage AA (2014) Melanin dependent survival of Apergillus fumigatus conidia in lung epithelial cells. (springer.com)
  • Dissemination of A. fumigatus occurs by release of asexual spores, called conidia, into the atmosphere. (genetics.org)
  • Ibrutinib is associated, both in vitro and in patients under treatment, with multiple functional defects in neutrophils, including decreased reactive oxygen species production, impairment of their capacity to engulf Aspergillus and inability to efficiently kill germinating conidia. (haematologica.org)
  • Coevolution of TH1, TH2, and TH17 responses during repeated pulmonary exposure to Aspergillus fumigatus conidia. (aaem.pl)
  • Structure-derived peptides were utilized to prepare anti-AFL polyclonal antibodies, which suggested the presence of AFL on the Aspergillus' conidia, confirming its expression in vivo. (archives-ouvertes.fr)
  • Using a mutant Aspergillus fumigatus strain (Δ rodA ) that does not express the RodA hydrophobin, and Aspergillus and Fusarium conidia from clinical isolates that were treated with hydrofluoric acid (which removes the A. fumigatus RodA protein), we observed increased surface exposure of β1,3-glucan and α-mannose on Aspergillus and Fusarium conidia. (jimmunol.org)
  • Using a murine model of A. fumigatus corneal infection, we showed that Δ rodA conidia induced significantly higher cytokine production, neutrophil infiltration, and more rapid fungal clearance from C57BL/6 corneas compared with the parent G10 strain, which was dependent on Dectin-1 and Dectin-2. (jimmunol.org)
  • A recent study showed that removal of Aspergillus fumigatus RodA by hydrofluoric acid (HF), which cleaves the phosphodiester bonds attaching RodA to the cell wall, confers conidia recognition by human dendritic cells and murine alveolar macrophages ( 5 ). (jimmunol.org)
  • We show that β1,3-glucan and α-mannan are exposed on the cell wall of Aspergillus conidia in the absence of RodA and that the C-type lectins Dectin-1 and Dectin-2 mediate the host response. (jimmunol.org)
  • Aspergillus fumigatus is identified microscopically by characteristic blue-green conidia borne on uniseriate conidiophores with subclavate vesicles. (asm.org)
  • The interaction between Aspergillus fumigatus (A. fumigatus) conidia and type II lung epithelial cells serves an important role in disease progression. (spandidos-publications.com)
  • Airway epithelial cells (including lung epithelial cells and tracheal epithelial cells) are the site of the initial process of A. fumigatus conidia. (spandidos-publications.com)
  • An In vitro study has demonstrated that A. fumigatus conidia first adhere to the surface of type II lung epithelial cells and are then endocytosed by lung epithelial cells, which triggers an immune response and respiratory burst ( 6 ). (spandidos-publications.com)
  • Interaction between Aspergillus fumigatus conidia and different proteins known to mediate the attachment of malignant tumor cells or microorganisms to the host tissues was studied in vitro. (asm.org)
  • The saprophytic mold Aspergillus fumigatus is found worldwide and has a high sporulating capacity, which results in the ubiquitous presence of high concentrations of conidia in the air ( 14 ). (asm.org)
  • The virulence of A. fumigatus is caused by the production of fungal proteins that promote mycelial growth into the lung parenchyma or by structural features of the conidia that confer resistance to the host's antifungal properties ( 13 ). (asm.org)
  • Thus, the aim of this study was to determine the transcriptomic response of the human bronchial epithelial cell line (16HBE14o-) following interaction with A. fumigatus conidia. (biomedcentral.com)
  • We used fluorescence-activated cell sorting (FACS) to separate 16HBE14o- cells having bound and/or internalized A. fumigatus conidia expressing green fluorescent protein from cells without spores. (biomedcentral.com)
  • Although A. fumigatus is not the most prevalent fungal species worldwide, it is one of the most ubiquitous fungi due to the large number of airborne conidia it releases. (biomedcentral.com)
  • One way to screen isolates of Aspergillus fumigatus for azole resistance is to use an agar plate assay that includes antifungal drugs in the agar. (cdc.gov)
  • The Centers for Disease Control and Prevention is interested in receiving US azole-resistant A. fumigatus isolates for surveillance. (cdc.gov)
  • This procedure will be used for screening Aspergillus fumigatus isolates for azole antifungal resistance using a 4-quadrant agar plate with antifungals. (cdc.gov)
  • This procedure outlines how to: 1) create 4-well screening plates, 2) process isolates, 3) inoculate Aspergillus screening plates and record the results, and 4) store the isolates. (cdc.gov)
  • To investigate azole resistance in clinical Aspergillus isolates, we conducted prospective multicenter international surveillance. (nih.gov)
  • A total of 3,788 Aspergillus isolates were screened in 22 centers from 19 countries. (nih.gov)
  • Correlation between the elastase activity index and invasiveness of clinical isolates of Aspergillus fumigatus ," Journal of Clinical Microbiology , vol. 40, no. 5, pp. 1811-1813, 2002. (hindawi.com)
  • A. fumigatus isolates resistant to medical triazoles have recently been identified in the United States in clinical and environmental specimens. (cdc.gov)
  • Of particular concern are resistant A. fumigatus isolates carrying either TR 34 /L98H or TR 46 /Y121F/T289A genetic resistance markers, which have been associated with environmental triazole fungicide use rather than previous patient exposure to antifungals ( 3 , 4 ). (cdc.gov)
  • In 2011, CDC initiated passive laboratory monitoring for U.S. triazole-resistant A. fumigatus isolates through outreach to clinical laboratories. (cdc.gov)
  • In recent years acquired azole resistance in Aspergillus fumigatus has been increasingly reported and a dominant mechanism of resistance (TR34/L98H) was found in clinical and environmental isolates. (eurosurveillance.org)
  • The aim of the present study was to investigate the prevalence of azole resistance in environmental A. fumigatus isolates collected in northern Italy. (eurosurveillance.org)
  • Azole-resistant isolates were detected in 13% (6/47) of the soil samples and in 21% (6/29) of the soil samples containing A. fumigatus. (eurosurveillance.org)
  • about the prevalence of azole-resistant Aspergillus fumigatus isolates from 19 countries, including 2 from the Americas (Brazil and the United States) ( 1 ). (cdc.gov)
  • However, clinical isolates from 22 states in the United States ( 3 ) and a few isolates from Latin America ( 1 , 4 ) failed to show any fungicide-driven resistance in A. fumigatus in these continents, even though use of pesticides is a widespread practice in the Americas. (cdc.gov)
  • Azole resistance in Aspergillus fumigatus isolates from the ARTEMIS global surveillance study is primarily due to the TR/L98H mutation in the cyp51A gene. (cdc.gov)
  • In a prior study, we identified seven clinical isolates of an Aspergillus sp. (asm.org)
  • These isolates were initially considered to be variants of Aspergillus fumigatus because of differences in mitochondrial cytochrome b sequences and unique randomly amplified polymorphic DNA PCR patterns (S. A. Balajee, M. Weaver, A. Imhof, J. Gribskov, and K. A. Marr, Antimicrob. (asm.org)
  • Results revealed that four of the seven variant isolates clustered together in a clade very distant from A. fumigatus and distinct from other members of the A. fumigatus group. (asm.org)
  • In the present study, we evaluated the phylogenetic relationships of these variant isolates to A. fumigatus and related species by multilocus sequence typing (MLST). (asm.org)
  • The novel cyclic hexapeptide ASP2397 (currently known as VL-2397) exhibited antifungal activity against Aspergillus fumigatus (including azole-sensitive and azole-resistant isolates), A. terreus, and A. flavus at a MIC range of 1 to 4 μg/ml in human serum. (medworm.com)
  • In this study, we analyzed 2,026 A. fumigatus isolates from 13 countries in four continents using nine highly polymorphic microsatellite markers. (ru.nl)
  • Genetic cluster analyses suggest that our global sample of A. fumigatus isolates belonged to eight genetic clusters, with seven of the eight clusters showing broad geographic distributions. (ru.nl)
  • 7 VCZ exhibited promising in vitro activity against 239 clinical isolates of Aspergillus spp. (arvojournals.org)
  • Aspergillus fumigatus is the most common airborne fungal pathogen of humans, causing life-threatening invasive disease in immunocompromised patients. (rupress.org)
  • IMPORTANCE Aspergillus fumigatus is the major filamentous fungal pathogen in humans. (asm.org)
  • We have evaluated the usefulness of parasexual genetics in the identification of genes essential for the growth of the human fungal pathogen Aspergillus fumigatus . (genetics.org)
  • Virulence of the fungal pathogen Aspergillus fumigatus is in part based on the saprophytic lifestyle that this mold has evolved. (asm.org)
  • The opportunistic fungal pathogen Aspergillus fumigatus is increasingly found as a coinfecting agent along with Pseudomonas aeruginosa in cystic fibrosis patients. (pubmedcentralcanada.ca)
  • Our previous studies on the class V myosin, MyoE, in the fungal pathogen Aspergillus fumigatus confirmed its requirement for normal morphology and virulence. (biologists.org)
  • IMPORTANCE The genetic diversity and geographic structure of the human fungal pathogen A. fumigatus have been the subject of many studies. (ru.nl)
  • Thermotolerance and virulence of Aspergillus fumigatus: role of the fungal nucleolus. (nih.gov)
  • This review will discuss the relationship between thermotolerance and virulence in pathogenic fungi, emphasizing the link to ribosome biogenesis in A. fumigatus. (nih.gov)
  • Genes and molecules involved in Aspergillus fumigatus virulence," Revista Iberoamericana de Micologia , vol. 22, no. 1, pp. 1-23, 2005. (hindawi.com)
  • Bultman KM, Kowalski CH, Cramer RA (2017) Aspergillus fumigatus virulence through the lens of transcription factors. (springer.com)
  • Conidial pigmentation of A. fumigatus significantly influences its virulence in a murine model. (asm.org)
  • In striking contrast, A. fumigatus l -ornithine- N 5 -monooxygenase (SidA), which catalyses the first committed step of hydroxamate-type siderophore biosynthesis, is absolutely essential for virulence. (rupress.org)
  • Thus, A. fumigatus SidA is an essential virulence attribute. (rupress.org)
  • The importance of the host immune status has been underlined by the lack of identified specific virulence attributes in A. fumigatus ( 1 , 3 - 6 ). (rupress.org)
  • These analyses suggest fumitremorgin C, pseurotin A and, putatively, a pigment augment A. fumigatus virulence. (bl.uk)
  • Genetic Analysis Using an Isogenic Mating Pair of Aspergillus fumigatus Identifies Azole Resistance Genes and Lack of MAT Locus's Role in Virulence. (jcvi.org)
  • We constructed a highly fertile isogenic pair of A. fumigatus strains with opposite mating types and used them to investigate whether mating type is associated with virulence and to find the genetic loci involved in azole resistance. (jcvi.org)
  • The survival curves of animals infected with either strain in three different animal models showed no significant difference, suggesting that virulence in A. fumigatus was not associated with mating type. (jcvi.org)
  • This approach can be applied to multiple areas of interest in A. fumigatus or other heterothallic pathogens, especially for virulence associated traits. (jcvi.org)
  • Based on recently gained insights into the cellular armamentarium that is expressed by members of this genus, the hypothesis that exclusive virulence factors contribute specifically to the virulome of A. fumigatus has been challenged. (asm.org)
  • Each subclass of proteolytic activity appears to be encoded not by one particular locus but by a family of genes, which hampers a conclusive assessment of their role in the virulence of A. fumigatus . (asm.org)
  • Resistance to A. fumigatus of mice lacking Bak compared to wild-type mice demonstrates the in vivo relevance of this GT-induced apoptotic pathway involving Bak and suggests a correlation between GT production and virulence. (rupress.org)
  • A multitude of putative A. fumigatus virulence factors, such as extracellular metalloprotease, serine protease, aspartic protease, catalase, phospholipases, haemolysin, and the cytotoxin ASPF1 have been implicated in IA, but none has yet been shown to be involved in the pathogenesis of A. fumigatus in experimentally induced infections ( Latge, 2001 ). (rupress.org)
  • The genome sequences of three Aspergillus species- Aspergillus fumigatus , Aspergillus nidulans , and Aspergillus oryzae -were published in Nature in December 2005. (wikipedia.org)
  • Large-scale genome comparisons have shown that no gene sets are shared exclusively by both Aspergillus fumigatus and any other human pathogen sequenced to date, such as Candida or Cryptococcus species. (nih.gov)
  • Aspergillus fumigatus was first officially described 1863 and MycoBank reports up to 17 varieties of this species. (inspq.qc.ca)
  • Samson RA, Gams W. Typification of the species of Aspergillus and associated teleomorphs In Samson RA, Gams W. Advances in Penicillium and Aspergillus systematics. (atcc.org)
  • Phylogenetic analysis of Aspergillus species using DNA sequences from four loci. (atcc.org)
  • Although this media can be used for the screening of any Aspergillus species for resistance to azoles, this procedure is written primarily for use with A. fumigatus . (cdc.gov)
  • The genus Aspergillus , composed of ca. 300 species, contains both pathogenic and saprophytic species which break spore dormancy through three distinct stages of spore maturation, collectively known as the germination process. (frontiersin.org)
  • We present the genome sequences of a new clinical isolate of the important human pathogen, Aspergillus fumigatus, A1163, and two closely related but rarely pathogenic species, Neosartorya fischeri NRRL181 and Aspergillus clavatus NRRL1. (jcvi.org)
  • Cross-species comparison has revealed that 8.5%, 13.5% and 12.6%, respectively, of A. fumigatus, N. fischeri and A. clavatus genes are species-specific. (jcvi.org)
  • Aspergillus species are common in the environment in dust, soil and vegetable material. (sciencephoto.com)
  • Though cytokines and phagocyte, as host factors, have been shown to participate in defence against Aspergillus species yet the role of cysteine proteases, that is cathepsins, a lysosomal enzymes of phagocytes, remains unknown in fungal infection. (ijpsonline.com)
  • Reports are also available where it is proved that Aspergillus species cause infections in patients who have defective phagocytic function [ 8 ]. (ijpsonline.com)
  • In other words, phagocytes, as one of the host factors, participate in body defence against Aspergillus species. (ijpsonline.com)
  • Other Aspergillus species (e.g. (springer.com)
  • Aspergillus species are natural haploids that reproduce asexually, although a sexual cycle is performed by some species, in particular A. nidulans , when diploids are transiently formed. (genetics.org)
  • Park SJ, Mehrad B. Innate immunity to Aspergillus species. (aaem.pl)
  • However, Aspergillus and Fusarium species are also major causes of corneal infections (keratitis), which occur in immune competent individuals and are a significant cause of blindness worldwide ( 3 ). (jimmunol.org)
  • Previously, we reported that neutrophils combat hyphal growth in the cornea through reactive oxygen species (ROS) and iron limitation and that A. fumigatus possesses antioxidant and iron acquisition mechanisms to allow for survival ( 7 , 8 ). (jimmunol.org)
  • Aspergillus and Fusarium species are important causes of fungal infections worldwide. (jimmunol.org)
  • As Aspergillus and Fusarium species are also major causes of corneal infection and blindness worldwide ( 6 ), the current study examined the role of hydrophobins in a murine model of fungal keratitis ( 7 , 8 ). (jimmunol.org)
  • Phenotype studies revealed that the variant isolate has smaller conidial heads with diminutive vesicles compared to A. fumigatus and is not able to survive at 48°C. Our findings suggest the presence of a previously unrecognized, potentially drug-resistant Aspergillus species that we designate A. lentulus . (asm.org)
  • However, A. fumigatus can be morphologically variable and several new species have been defined within this group on the basis of minor phenotypic differences. (asm.org)
  • Morphological and phylogenetic characterization of this new species in the A. fumigatus series is presented. (asm.org)
  • This distribution of alkaloids is consistent with the hypothesis that early steps of the ergot alkaloid pathways are shared by these diverse fungal species but the later portions of the pathways are different in A. fumigatus and the clavicipitaceous fungi. (asm.org)
  • ASP2397, a novel natural compound that exhibits rapid and potent fungicidal activity against Aspergillus species through a specific transporter. (medworm.com)
  • Because of the ubiquity of Aspergillus species it is difficult to decide between contamination by commensalism or a serious infection. (blockscientificstore.com)
  • Aspergillus fumigatus pneumonia in neutropenic patients receiving fuconazole for infection due to candida species: Is amphotericin B combined with fluconazole the appropriate answer? (uzh.ch)
  • The AsperGenius® assay detects several Aspergillus species and the A. fumigatus Cyp51A mutations TR34/L98H/T289A/Y121F that are associated with azole resistance. (eur.nl)
  • We evaluated its contribution in identifying A. lentulus and A. felis, 2 rare but intrinsically azole-resistant sibling species within the Aspergillus section Fumigati. (eur.nl)
  • Aspergillus fumigatus is the most frequent cause of invasive fungal infection in immunosuppressed individuals, which include patients receiving immunosuppressive therapy for autoimmune or neoplastic disease, organ transplant recipients, and AIDS patients. (wikipedia.org)
  • [11] A. fumigatus primarily causes invasive infection in the lung and represents a major cause of morbidity and mortality in these individuals. (wikipedia.org)
  • However, in healthy individuals, the innate immune system is an efficacious barrier to A. fumigatus infection. (wikipedia.org)
  • A. fumigatus spores are known to be carried long distances in the air, putting patients at risk for infection with resistant strains, even in areas without known agricultural fungicide usage. (cdc.gov)
  • Infection with A. fumigatus resulted in only 62 up and 47 down-regulated genes in A549. (springer.com)
  • This study has conducted global Aspergillus fumigatus transcriptional analyses throughout the initiation of murine infection using a wild-type and an attenuated ΔlaeA isolate. (bl.uk)
  • Molecular insights into pathogenesis and infection with Aspergillus fumigatus . (springer.com)
  • But increasing use of immunosuppressive therapy has resulted in increasingly frequent conditions involving infection by A fumigatus . (phadia.com)
  • Using a murine model of Aspergillus corneal infection, we also demonstrate that in the absence of RodA, A. fumigatus induces Dectin-1- and Dectin-2-dependent neutrophil recruitment to the corneal stroma and enhanced fungal killing. (jimmunol.org)
  • In a recent study, we identified several poorly sporulating variants of A. fumigatus as a cause of invasive infection in hematopoietic stem cell transplant recipients ( 1 ). (asm.org)
  • Innate and adaptive immune response to chronic pulmonary infection of hyphae of Aspergillus fumigatus in a new murine model. (medworm.com)
  • The present study compared the proteomes of A549 human lung epithelial cells with and without A. fumigatus infection. (spandidos-publications.com)
  • Activity of liposomal nystatin against disseminated Aspergillus fumigatus infection in neutropenic mice. (asm.org)
  • The purpose of this study was to examine the activity of liposomal nystatin against a disseminated Aspergillus fumigatus infection in neutropenic mice. (asm.org)
  • Based on these results in mice, it is probable that liposomal nystatin will be effective against Aspergillus infection in humans. (asm.org)
  • The recent discovered role of glycogen synthase kinase-3 homologue, GskA, in A. fumigatus human infection and our previous experience on human GSK-3 inhibitors focus our attention on this kinase as a target for the development of antifungal drugs. (csic.es)
  • These observations might have implications for treatment of A. fumigatus colonization in chronic lung diseases and for its potential impact on airway inflammation, damage, and infection. (asm.org)
  • Although infections by A. fumigatus have been described in other sites of the body, the respiratory tract is the main route of entry and site of infection. (biomedcentral.com)
  • The mycotoxin gliotoxin that is produced by A. fumigatus and has antiphagocytic activity was not detected in the spores from any of the A. fumigatus strains. (eurekamag.com)
  • Binding of Aspergillus fumigatus spores to lung epithelial cells and basement membrane proteins: relevance to the asthmatic lung. (bmj.com)
  • The ability of spores of A fumigatus to bind to pulmonary cells and basement membrane proteins was investigated to determine the mechanisms involved in this susceptibility. (bmj.com)
  • They were then exposed to spores of A fumigatus in suspension, with or without various pretreatments of the spores, cells, and proteins. (bmj.com)
  • RESULTS: Spores of A fumigatus bound in a concentration dependent manner to A549 epithelial cells and pretreatment of cells with interferon gamma (2500 units/ml) caused a significant doubling of spore binding. (bmj.com)
  • Free fibrinogen may protect against binding of A fumigatus spores to the pulmonary epithelium. (bmj.com)
  • Reports of these triazole-resistant A. fumigatus strains have become common in Europe ( 2 , 3 ), but U.S. reports are limited ( 5 ). (cdc.gov)
  • Samples were inoculated on Sabouraud agar at 43°C, and positive samples were screened for azole-resistance on agar supplemented with either itraconazole (4 mg/L) or voriconazole (4 mg/L). Of the 38 resistant Aspergillus strains, 20 were selected (up to 5 colonies for each positive culture), identified as A. fumigatus by β-tubulin gene sequencing, and analyzed for CYP51A gene alterations ( 7 ). (cdc.gov)
  • Clinical management of Aspergillus disease can be challenging because of unfavorable clinical outcomes after patients have acquired multi-azole-resistant strains from the environment ( 9 ). (cdc.gov)
  • Here, we investigate which pathways are influenced by A. fumigatus AfCrzA during a short pulse of calcium by comparatively determining the transcriptional profile of A. fumigatus wild type and ΔAfcrzA mutant strains. (csic.es)
  • The discovery of highly fertile strains of A. fumigatus opened the possibility to merge classical and contemporary genetics to address key questions about this pathogen. (jcvi.org)
  • Five strains of Aspergillus fumigatus (A, B, D, H, and K) isolated from sawmills were used to expose groups of three rats by intratracheal intubation. (eurekamag.com)
  • There was an obvious difference in pathogenicity among the strains of A. fumigatus. (eurekamag.com)
  • The in vitro antifungal activities of seven antifungal drugs were tested by comparing planktonic and sessile A. fumigatus strains. (asm.org)
  • All five Aspergillus fumigatus strains resistant itraconazole with reduced susceptibility to voriconazole and posaconazole carried carrying TR34/L98H resistance allele. (springer.com)
  • In this paper, we characterize a global collection of strains of A. fumigatus using a panel of 9 highly polymorphic microsatellite markers. (ru.nl)
  • The Aspergillus screening plates contains four quadrants with RPMI 1640 2% glucose agar: one growth control well with no antifungal powder, one voriconazole well, one itraconazole well, and one posaconazole well. (cdc.gov)
  • Aspergillus fumigatus has widely evolved resistance to the most commonly used class of antifungal chemicals, the azoles. (asm.org)
  • Triazole antifungal medications are the primary treatment for invasive A. fumigatus infections, opportunistic infections that typically affect immunocompromised patients. (cdc.gov)
  • the newly presented data and insights not only sharpen our image of this multicellular stage within the Aspergillus lifestyle but also provide perspective for improvement and new avenues in antifungal therapy or biotechnological applications. (frontiersin.org)
  • A decraesing quantitiy of Aspergillus-fumigatus-DNA could be detected after antifungal treatment and connected with the decreasing suspicion of an IA in the other groups. (hu-berlin.de)
  • Though antifungal drug market is in boom and an increased number of antifungal options significantly decreased the complications in patients with serious Aspergillus infections even than success rate to cure this disease is very low [ 4 , 5 ]. (ijpsonline.com)
  • Infections associated with A. fumigatus are often treated with azole drugs, but resistance to these antifungal agents is increasing. (asm.org)
  • Our results suggest that global populations of A. fumigatus are shaped by historical differentiation, contemporary gene flow, sexual reproduction, and the localized antifungal drug selection that is driving clonal expansion of genotypes resistant to multiple triazole drugs. (ru.nl)
  • Our analyses suggest that A. fumigatus contains historically differentiated genetic populations but that its evolution is significantly impacted by contemporary forces such as widespread gene flow and local antifungal drug pressure. (ru.nl)
  • Voriconazole (VCZ) is a second-generation, synthetic triazole with broad-spectrum antifungal activity against many medically important pathogens, including Aspergillus spp. (arvojournals.org)
  • Emergence of azole resistance in Aspergillus fumigatus and spread of a single resistance mechanism. (cdc.gov)
  • Serum beta-D-glucan (61.0 pg/μL) and Aspergillus galactomannan antigens in the serum and cerebrospinal fluid (CSF) (both ≥5.0) were elevated. (medworm.com)
  • A. fumigatus secretes an array of complex biologically-active glycoprotein antigens and allergens. (phadia.com)
  • ABPA is a pulmonary disorder of cystic fibrosis (CF) and asthma patients arising from allergic response to multiple antigens expressed by A. fumigatus colonizing the bronchial mucus. (asm.org)
  • Aspergillus collagen-like (acl) genes: identification, sequence polymorphism and assessment for PCR-based pathogen detection. (atcc.org)
  • Molecular phylogenetics of multiple genes on Aspergillus section Fumigati isolated from clinical specimens in Japan. (atcc.org)
  • Thus, these biochemical data are in agreement with a comparative genome analysis that shows that all but 3 of the 21 genes described in the Saccharomyces cerevisiae GPI pathways are found in A. fumigatus. (biomedsearch.com)
  • Paralelamente, para a RNAi, um fragmento do gene Afpmr1 apresentando baixa identidade com outros genes de cálcio ATPases de diferentes espécies foi clonado em vetor de expressão em A. fumigatus (pALB1). (usp.br)
  • At the same time, a fragment of the Afpmr1 gene, showing low identity values for another calcium ATPase genes, was cloned in an A. fumigatus expression vector (pALB1) for RNAi. (usp.br)
  • Comparative genomic analysis of A1163 with the recently sequenced A. fumigatus isolate Af293 has identified core, variable and up to 2% unique genes in each genome. (jcvi.org)
  • At least 20% of A. fumigatus-specific genes appear to be functional and involved in carbohydrate and chitin catabolism, transport, detoxification, secondary metabolism and other functions that may facilitate the adaptation to heterogeneous environments such as soil or a mammalian host. (jcvi.org)
  • A total of 545 and 473 genes of A. fumigatus and A. niger , respectively, were differentially expressed when compared to growth in the absence of A549 cells. (springer.com)
  • The orthologues of A. fumigatus genes involved in hypoxia regulation and heat shock were also up-regulated in A. niger, whereas thioredoxin reductase and allergen genes were found up-regulated in A. fumigatus but down-regulated in A. niger . (springer.com)
  • In the present study, six genes, forming a gene cluster spanning 19 kb, were identified as involved in conidial pigment biosynthesis in A. fumigatus . (asm.org)
  • Involvement of the six genes in conidial pigmentation was confirmed by the altered conidial color phenotypes that resulted from disruption of each gene in A. fumigatus . (asm.org)
  • However, significant plasmid and genomic DNA rearrangements were observed at many of the identified genomic loci where plasmid integration had occurred, thus suggesting that the use of electroporation to build libraries of A. fumigatus insertional mutants has relatively limited value and cannot be used in an exhaustive search of essential genes. (genetics.org)
  • AfCrzA overexpression in A. fumigatus increases the expression of several of these genes. (csic.es)
  • Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus . (springer.com)
  • Clustered with dmaW in the A. fumigatus genome are sequences corresponding to five genes previously proposed to encode steps in the ergot alkaloid pathway of C. purpurea , as well as additional sequences whose deduced protein products are consistent with their involvement in the ergot alkaloid pathway. (asm.org)
  • Aspergillus fumigatus proliferation and pathogenesis-conditionally speaking. (springer.com)
  • A repeated DNA sequence used for epidemiological studies of the human opportunistic pathogen Aspergillus fumigatus has been characterized. (nih.gov)
  • This does not happen if the pathogen Aspergillus fumigatus is involved. (news-medical.net)
  • The opportunistic human pathogen Aspergillus fumigatus initiates invasive growth through a programmed germination process that progresses from dormant spore to swollen spore (SS) to germling (GL) and ultimately invasive hyphal growth. (frontiersin.org)
  • However, the opportunistic pathogen Aspergillus fumigatus has developed worldwide resistance to azoles largely through mutations in the cytochrome P450 enzyme Cyp51 (Erg11). (asm.org)
  • A. fumigatus causes more infections worldwide than any other mould. (sanger.ac.uk)
  • A. fumigatus has been associated with each and every type of health problems linked to environmental moulds: irritation and inflammation, allergy, asthma, pneumonitis, toxic effects as well as a wide range of infections. (inspq.qc.ca)
  • however, triazole-resistant A. fumigatus infections are increasingly reported worldwide and are associated with increased treatment failure and mortality. (cdc.gov)
  • U.S. clinicians and public health personnel should be aware that infections with triazole-resistant A. fumigatus can occur in patients not previously exposed to these medications. (cdc.gov)
  • A. fumigatus resistant to all triazole medications is emerging in the United States, and clinicians and public health personnel need to be aware that resistant infections are possible even in patients not previously exposed to these medications. (cdc.gov)
  • Susceptibility testing is essential to inform the correct management of Aspergillus infections. (scielo.br)
  • A. fumigatus is can cause a number of different disease states in humans including allergic disorders, respiratory infections and invasive disease. (sciencephoto.com)
  • Other aspergilli like Aspergillus niger also cause infections but to a much lesser extent. (springer.com)
  • Aspergillus fumigatus causes severe pulmonary and disseminated infections in patients with compromised immune systems because of solid organ and bone marrow transplant, HIV/AIDS, and genetic immunodeficiencies ( 1 , 2 ). (jimmunol.org)
  • In general infections with Aspergillus spp. (blockscientificstore.com)
  • Aspergillus fumigatus infections cause high levels of morbidity and mortality in immunocompromised patients. (rupress.org)
  • The elucidation of the molecular basis opens new strategies for the development of therapeutic regimens to combat A. fumigatus and related fungal infections. (rupress.org)
  • The true incidence of A. fumigatus infections is underestimated because of the inherent difficulty of positive diagnosis ( Latge, 1999 ). (rupress.org)
  • Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. (broadinstitute.org)
  • Here we report the genome sequence of the model organism Aspergillus nidulans, and a comparative study with Aspergillus fumigatus, a serious human pathogen, and Aspergillus oryzae, used in the production of sake, miso and soy sauce. (broadinstitute.org)
  • Etxebeste O, Garzia A, Espeso EA, Ugalde U (2010) Aspergillus nidulans asexual development: making the most of cellular modules. (springer.com)
  • The abr2 gene product has homology to the laccase encoded by the yA gene of Aspergillus nidulans . (asm.org)
  • We have shown that l -ornithine- N 5 -monooxygenase catalyzes the first committed step in biosynthesis of both triacetylfusarinine C (TAFC) and desferriferricrocin (DFFC), the two major siderophores of Aspergillus nidulans ( 18 ). (rupress.org)
  • Concomitantly with A. fumigatus AfrcnA molecular analysis, we decided to exploit the conserved features of A. nidulans calcineurin system and investigated the A. nidulans AnRcnA homologue. (csic.es)
  • The aim of this work is, using Aspergillus nidulans as non-pathogen model, to develop efficient drugs to treat IA. (csic.es)
  • With the aim to identify effective inhibitors of colonial growth of A. fumigatus we use A. nidulans as an accurate model for in vivo and in silico studies. (csic.es)
  • Aspergillus fumigatus is an opportunistic human pathogen that causes various complications in patients with a weakened immune system functions. (springer.com)
  • C. M. Tang, J. Cohen, and D. W. Holden, "An Aspergillus fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity," Molecular Microbiology , vol. 6, no. 12, pp. 1663-1671, 1992. (hindawi.com)
  • The most common pathogen of the genus Aspergillus is A. fumingatus occuring in hay, grain, rotten plants and bird faeces. (blockscientificstore.com)
  • Diseases caused by the fungal pathogens of the genus Aspergillus represent a growing and clinically relevant threat in distinct medical settings ( 31 ). (asm.org)
  • The study isolated 48 yielded Aspergillus fumigatus and 2 A. flavus . (hindawi.com)
  • Our previous studies showed that A. fumigatus and A. niger have different behavior in the presence of type II alveolar A549 epithelial cells. (springer.com)
  • Our dual transcriptomic analysis supports earlier observations of a marked difference in life style between A. fumigatus and A. niger when grown in the presence of type II epithelial cells. (springer.com)
  • CONCLUSIONS: This study suggests that the presence of activated epithelial cells and the exposure of basement membrane that occurs in asthma, together with oxidant stress, may facilitate the colonisation of the asthmatic lung by A fumigatus. (bmj.com)
  • The proteomes of A549 type II lung epithelial cells with and without A. fumigatus challenge were compared using the isobaric tags for relative and absolute quantification (iTRAQ) technique. (spandidos-publications.com)
  • We investigated the ability of A. fumigatus to form biofilms on polystyrene (PS) and human bronchial epithelial (HBE) and CF bronchial epithelial (CFBE) cells. (asm.org)
  • A. fumigatus toxins have been shown to inhibit ciliary activity, and proteases can damage the epithelial tissue ( 1 ). (asm.org)
  • To investigate the expression and function of Mannose Receptor (MR) and explore its interaction with Dectin-1 in human corneal epithelial cells (HCECs) exposed to Aspergillus fumigatus ( A. fumigatus ). (arvojournals.org)
  • In vitro biosynthesis of glycosylphosphatidylinositol in Aspergillus fumigatus. (biomedsearch.com)
  • Based on DNA sequence similarity, gene disruption results, and biochemical analyses, we conclude that the 19-kb DNA fragment contains a six-gene cluster which is required for conidial pigment biosynthesis in A. fumigatus . (asm.org)
  • However, the presence of abr1 , abr2 , and ayg1 in addition to alb1 , arp1 , and arp2 suggests that conidial pigment biosynthesis in A. fumigatus is more complex than the known DHN-melanin pathway. (asm.org)
  • Aspergillus fumigatus has a stable haploid genome of 29.4 million base pairs . (wikipedia.org)
  • The Sanger Institute was then funded to carry our a whole genome shotgun of A. fumigatus in collaboration with The Institute of Genome Research (TIGR). (sanger.ac.uk)
  • The table below summarised the type, genome location and corresponding target sites of the ncRNAs identified in A. fumigatus. (wikipedia.org)
  • Sequencing the Aspergillus fumigatus genome. (springer.com)
  • A homolog of dmaW , the gene controlling the determinant step in the ergot alkaloid pathway of clavicipitaceous fungi, was identified in the A. fumigatus genome. (asm.org)
  • Among those, proteases appear to play a prominent role, which is indicated by the huge array of proteolytic activities that are presumably encoded by the A. fumigatus genome to comprise more than 100 proteases ( 33 , 36 ). (asm.org)
  • The crystal structure of Aspergillus fumigatus cyclophilin (Asp f 11) was solved by the multiwavelength anomalous dispersion method and was refined to a resolution of 1.85 A with R and R(free) values of 18.9% and 21.4%, respectively. (rcsb.org)
  • Our study investigated the response to Aspergillus fumigatus and neutrophil function in patients with chronic lymphoid leukemia or lymphoma, who were undergoing ibrutinib therapy. (haematologica.org)
  • For all 8 patients with proven IA, the detection of Aspergillus-fumigatus-DNA succeeded. (hu-berlin.de)
  • Collectively, these studies demonstrate a novel stage-specific susceptibility of A. fumigatus to zinc and manganese chelation by neutrophil-derived calprotectin. (jimmunol.org)
  • We demonstrate a methodology using FACs for analyzing the transcriptome of infected and uninfected cells from the same cell population that will provide a framework for future characterization of the specific interactions between pathogens such as A. fumigatus with human cells derived from individuals with or without underlying disease susceptibility. (biomedcentral.com)
  • For one of the major fungal pathogens, the environmental mold Aspergillus fumigatus , an interesting and relevant morphotype beyond the conidial and hyphal stages has gained increasing attention: the multicellular biofilm. (frontiersin.org)
  • The major pathogens are Blastomyces dermatitidis , Aspergillus spp. (arvojournals.org)
  • A close association was found between pulmonary function and clinical state, and IgG antibodies to Aspergillus. (bmj.com)
  • Although hypersensitivity to Aspergillus fumigatus ( A fum ) was not measured here, two other studies have reported on the higher prevalence of A fum sensitization in COPD and its association with worse lung function. (dovepress.com)
  • Also, mice infected with an A. fumigatus ΔrodA mutant caused increased lung inflammation compared with the parent strain ( 5 ). (jimmunol.org)
  • First, essentiality of the A. fumigatus AfFKS1 gene, encoding the catalytic subunit of the β-(1,3)-glucan synthase complex, was assessed by inactivating one allele of AfFKS1 in a diploid strain of A. fumigatus obtained using adequate selectable markers in spore color and nitrate utilization pathways and by performing haploidization under conditions that select for the occurrence of the disrupted allele. (genetics.org)
  • Conclusion] We have performed a transcriptional profiling analysis of the A. fumigatus ΔAfcrzA mutant strain exposed to calcium stress. (csic.es)
  • Here, we describe the construction of a bioluminescent A. fumigatus strain by fusing the promoter of the glyceraldehyde-3-phosphate dehydrogenase gene from A. fumigatus with the luciferase gene from Photinus pyralis to control the expression of the bioluminescent reporter. (biomedsearch.com)
  • The Afmac1 deletion strain of A. fumigatus failed to grow in copper-limited media, and its growth was restored by introducing ctrC . (biochemj.org)
  • A. fumigatus strain IFM40808 was provided by the Medical Mycology Research Center of Chiba University (Chiba, Japan). (spandidos-publications.com)
  • Aspergillus fumigatus grown on certain building materials can produce genotoxic and cytotoxic mycotoxins , such as gliotoxin . (wikipedia.org)
  • Among other factors, it is gliotoxin - a potent mycotoxin - that is responsible for the pathogenicity of Aspergillus fumigatus. (news-medical.net)
  • El equipo de investigación de la universidad de Jena (Alemania) clarifica el mecanismo del gliotoxin, una micotoxina del fumigatus fungoso del aspergillus. (news-medical.net)
  • Entre otros factores, es gliotoxin - una micotoxina potente - que es responsable de la patogenicidad del fumigatus del aspergillus. (news-medical.net)
  • Para lograr esto, los investigadores trajeron las células inmunes en contacto con el gliotoxin sintetizadamente producido. (news-medical.net)
  • Pues los científicos de Jena podían mostrar, el gliotoxin se asegura de que la producción de la substancia leukotrieneB4 del mensajero en los granulocytes neutrophilic esté inhibida, de modo que no puedan enviar una señal a otras células inmunes. (news-medical.net)
  • In contrast, the structure of A. fumigatus cyclophilin reveals dimerization by 3D domain swapping and represents one of the first proteins with a swapped central domain. (rcsb.org)
  • Proteomic analysis of temperature dependent extracellular proteins from Aspergillus fumigatus grown under solid-state culture condition. (springer.com)
  • A. fumigatus can produce cytotoxic and immunosuppressive proteins that enable it to persist for long periods in the respiratory tract, particularly in airflow obstructions and impaired clearance mechanisms ( 14 ). (asm.org)
  • Biochemical characterization of extracellular fructosyltransferase from Aspergillus oryzae IPT-301 immobilized on silica gel for the production of fructooligosaccharides. (medworm.com)
  • A. fumigatus formed an extracellular matrix on PS and HBE and CFBE cells as evidenced by increased dry weight, SEM, and CSLM. (asm.org)
  • However, recent data indicate that A. fumigatus survival in human serum in vitro involves siderophore-mediated removal of iron from transferrin ( 17 ). (rupress.org)
  • We also found that an A. fumigatus ∆ zafA mutant, which demonstrates deficient zinc transport, exhibits impaired growth in infected corneas and following incubation with neutrophils or calprotectin in vitro as compared with wild-type. (jimmunol.org)
  • We developed a novel in vitro coculture model of A. fumigatus biofilm formation on HBE and CFBE cells. (asm.org)
  • In order to dissect alterations of the immune system involved with both aging and chronic exposure to A. fumigatus , we used 3- and 18-month-old C57BL/6J mice exposed to repeated A. fumigatus inhalations for 7 and 28 days. (aaem.pl)
  • Aspergillus fumigatus is often associated in asthmatic patients with the exacerbation of asthma symptoms. (sigmaaldrich.com)
  • To answer this question, we studied the consequences of ibrutinib exposure on the functions and anti-Aspergillus responses of neutrophils obtained from healthy donors and 63 blood samples collected at different time points amongst 32 patients receiving ibrutinib for lymphoid malignancies. (haematologica.org)
  • We hypothesized that sensitization to Aspergillus fumigatus ( A fum ) is associated with bronchiectasis in COPD and occurs more frequently in vitamin D-deficient patients. (dovepress.com)
  • To understand resistance selection, the life cycle of A. fumigatus needs to be investigated from its natural habitat to the disease it causes in patients: from molecule to man to population. (aspergillus.org.uk)
  • Mast cell tryptase changes with Aspergillus fumigatus - Host crosstalk in cystic fibrosis patients. (ecfs.eu)
  • Aspergillus fumigatus is a leading cause of death in immunocompromised patients and a frequent colonizer of the respiratory tracts of asthma and cystic fibrosis (CF) patients. (asm.org)
  • Serum was collected from 50 patients with cystic fibrosis, and IgG antibodies to Aspergillus fumigatus were measured by enzyme linked immunosorbent assay (ELISA). (bmj.com)
  • It is postulated that in patients with cystic fibrosis, Aspergillus fumigatus may contribute to deterioration in pulmonary function by local pathogenicity, or by hypersensitivity mechanisms mediated by IgG. (bmj.com)
  • Using a quantitative PCR assay specific for Aspergillus fumigatus 28S ribosomal DNA, we retrospectively tested 422 GM-positive (Platelia Bio-Rad kit) serum samples collected over 1 year from 147 patients. (pasteur.fr)
  • Desta forma, o objetivo do presente estudo foi a expressão, caracterização e validação como alvo quimioterapêutico do gene Afpmr1 de A. fumigatus. (usp.br)
  • Estes resultados mostram que o gene Afpmr1 pode ser expresso funcionalmente em sistemas heterólogos e seu silenciamento, em A. fumigatus, influencia processos celulares que podem estar relacionados à manutenção da estrutura e composição da parede celular, além de desencadear alterações na fagocitose e killing de macrófagos. (usp.br)
  • In this way, the aim of the present work was the expression, characterization e validation, as chemotherapeutic target, of the A. fumigatus Afpmr1 gene. (usp.br)
  • These results show that the Afpmr1 gene can be functionally expressed in eukaryotic heterologous systems and its silencing, in A. fumigatus, alters cellular processes that can be related with the maintenance of the cell wall structure and composition, as well as promote alterations in the macrophages phagocytosis and killing. (usp.br)
  • Here it is shown that global gene expression of A. fumigatus and A. niger is markedly different upon contact with A549 cells. (springer.com)
  • A new azole resistance mechanism in Aspergillus fumigatus consisting of a TR46/Y121F/T289A alteration in the cyp51A gene was recently described in the Netherlands. (eurosurveillance.org)
  • The gene products of alb1 (for "albino 1"), arp1 (for "aspergillus reddish-pink 1"), and arp2 have high similarity to polyketide synthases, scytalone dehydratases, and hydroxynaphthalene reductases, respectively, found in the dihydroxynaphthalene (DHN)-melanin pathway of brown and black fungi. (asm.org)
  • The abr1 gene (for "aspergillus brown 1") encodes a putative protein possessing two signatures of multicopper oxidases. (asm.org)
  • Genomic analysis of these heterozygous diploids led in particular to the identification of an essential A. fumigatus gene encoding an SMC-like protein resembling one in Schizosacccharomyces pombe involved in chromosome condensation and cohesion. (genetics.org)
  • All of these variants were initially identified as A. fumigatus by morphological characteristics, but they had distinct mitochondrial cytochrome b (mtcytb) gene sequences and randomly amplified polymorphic DNA PCR patterns. (asm.org)
  • Recent reports have suggested a link between use of fungicides in agricultural practices and the presence of triazole-resistant A. fumigatus among azole-naive persons ( 2 ). (cdc.gov)
  • The prevalence of serum IgE antibodies to Dermatophagoides pteronyssinus (house-dust mite), Aspergillus fumigatus, and bovine beta-lactoglobulin was significantly greater in cases of sudden infancy death (S.I.D.) than in a control group of infants of the same age range. (bmj.com)
  • Enzyme immunoassays for the qualitative and quantitative determination of IgM antibodies against Aspergillus fumigatus in human serum and plasma. (blockscientificstore.com)
  • Circulating Aspergillus fumigatus DNA Is Quantitatively Correlated to Galactomannan in Serum. (pasteur.fr)
  • Environment sensing, counteracting reactive oxidants, and retrieving essential nutriments from the environment are general metabolic traits that are associated with the growth of the saprotrophic mold A. fumigatus in an unfriendly environment such as its human host. (nih.gov)
  • Azole resistance is an emerging problem in Aspergillus fumigatus , a saprophytic mold that causes a spectrum of diseases in animals and humans. (aspergillus.org.uk)
  • IgE antibodies to Dermatophagoides pteronyssinus (Housedust mite), Aspergillus fumigatus, and beta-lactoglobulin in sudden infant death syndrome. (bmj.com)
  • Turner K J , Baldo B A , Hilton J M . IgE antibodies to Dermatophagoides pteronyssinus (Housedust mite), Aspergillus fumigatus, and beta-lactoglobulin in sudden infant death syndrome. (bmj.com)
  • Next to the ELISA the indirect Aspergillus hemagglutination test (Aspergillus HAT) can be performed to detect specific IgG and IgM antibodies. (blockscientificstore.com)
  • IgG antibodies to Aspergillus fumigatus in cystic fibrosis: a laboratory correlate of disease activity. (bmj.com)
  • In addition, total IgE and Aspergillus specific IgE antibodies were measured in 41 of the 50. (bmj.com)
  • IgG antibodies to Aspergillus fumigatus in cystic fibrosis. (bmj.com)
  • Preparations having the ability to inhibit the growth of Sarcoma 180 in mice have been extracted from the culture filtrate and mycelial pads of Aspergillus fumigatus . (aacrjournals.org)
  • The study investigates the effects of the repeated exposure to A. fumigatus in mice pulmonary compartment. (aaem.pl)
  • Our study demonstrated that chronic inflammation in pulmonary compartment, characterized by the significant increase of proinflammatory cytokines (IL1, IL6, IL10) levels, was the dominant feature of mice response to repeated A. fumigatus inhalations. (aaem.pl)
  • Liposomal nystatin, at doses as low as 2 mg/kg of body weight/day, protected neutropenic mice against Aspergillus-induced death in a statistically significant manner at the 50-day time point compared to either the no-treatment, the saline, or the empty-liposome group. (asm.org)
  • Aspergillus fumigatus is made up of fungal threads (hyphae, not seen) with conidiophores (fruiting bodies, lower left) at the tip. (sciencephoto.com)
  • Aspergillus molds consist of septate hyphae and asexual fruiting bodies borne on conidiophores. (vetstream.com)
  • direct mycological examination revealing septate hyphae, consistent with Aspergillus sp. (ufrgs.br)
  • Knockout of dmaW eliminated all known ergot alkaloids from A. fumigatus , and complementation of the mutation restored ergot alkaloid production. (asm.org)