Aspergillosis, Allergic Bronchopulmonary
Infections of the respiratory tract with fungi of the genus ASPERGILLUS. Infections may result in allergic reaction (ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS), colonization in pulmonary cavities as fungus balls (MYCETOMA), or lead to invasion of the lung parenchyma (INVASIVE PULMONARY ASPERGILLOSIS).
Lung Diseases, Fungal
Invasive Pulmonary Aspergillosis
Drug Resistance, Fungal
Cyclic hexapeptides of proline-ornithine-threonine-proline-threonine-serine. The cyclization with a single non-peptide bond can lead them to be incorrectly called DEPSIPEPTIDES, but the echinocandins lack ester links. Antifungal activity is via inhibition of 1,3-beta-glucan synthase production of BETA-GLUCANS.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Gene Expression Regulation, Fungal
Microbial Sensitivity Tests
Drug Resistance, Multiple, Fungal
Infections of the nervous system caused by fungi of the genus ASPERGILLUS, most commonly ASPERGILLUS FUMIGATUS. Aspergillus infections may occur in immunocompetent hosts, but are more prevalent in individuals with IMMUNOLOGIC DEFICIENCY SYNDROMES. The organism may spread to the nervous system from focal infections in the lung, mastoid region, sinuses, inner ear, bones, eyes, gastrointestinal tract, and heart. Sinus infections may be locally invasive and enter the intracranial compartment, producing MENINGITIS, FUNGAL; cranial neuropathies; and abscesses in the frontal lobes of the brain. (From Joynt, Clinical Neurology, 1998, Ch 27, pp62-3)
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Furano-furano-benzopyrans that are produced by ASPERGILLUS from STERIGMATOCYSTIN. They are structurally related to COUMARINS and easily oxidized to an epoxide form to become ALKYLATING AGENTS. Members of the group include AFLATOXIN B1; aflatoxin B2, aflatoxin G1, aflatoxin G2; AFLATOXIN M1; and aflatoxin M2.
Animals, Outbred Strains
Eye Infections, Fungal
Infection by a variety of fungi, usually through four possible mechanisms: superficial infection producing conjunctivitis, keratitis, or lacrimal obstruction; extension of infection from neighboring structures - skin, paranasal sinuses, nasopharynx; direct introduction during surgery or accidental penetrating trauma; or via the blood or lymphatic routes in patients with underlying mycoses.
Amino Acid Sequence
Granulomatous Disease, Chronic
A defect of leukocyte function in which phagocytic cells ingest but fail to digest bacteria, resulting in recurring bacterial infections with granuloma formation. When chronic granulomatous disease is caused by mutations in the CYBB gene, the condition is inherited in an X-linked recessive pattern. When chronic granulomatous disease is caused by CYBA, NCF1, NCF2, or NCF4 gene mutations, the condition is inherited in an autosomal recessive pattern.
An NADPH-dependent P450 enzyme that plays an essential role in the sterol biosynthetic pathway by catalyzing the demethylation of 14-methyl sterols such as lanosterol. The enzyme acts via the repeated hydroxylation of the 14-methyl group, resulting in its stepwise conversion into an alcohol, an aldehyde and then a carboxylate, which is removed as formic acid. Sterol 14-demethylase is an unusual cytochrome P450 enzyme in that it is found in a broad variety of organisms including ANIMALS; PLANTS; FUNGI; and protozoa.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Cytochrome P-450 Enzyme System
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
A genus of yeast-like mitosporic Saccharomycetales fungi characterized by producing yeast cells, mycelia, pseudomycelia, and blastophores. It is commonly part of the normal flora of the skin, mouth, intestinal tract, and vagina, but can cause a variety of infections, including CANDIDIASIS; ONYCHOMYCOSIS; vulvovaginal candidiasis (CANDIDIASIS, VULVOVAGINAL), and thrush (see CANDIDIASIS, ORAL). (From Dorland, 28th ed)
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Association of a myosin immunoanalogue with cell envelopes of Aspergillus fumigatus conidia and its participation in swelling and germination. (1/1653)A myosin immunoanalogue was identified in conidia of Aspergillus fumigatus by Western blotting, indirect immunofluorescence assay, and gold immunoelectron microscopy with two different antimyosin antibodies. The distribution pattern of this protein was followed during the early stages of germination. A single 180-kDa polypeptide, detected predominantly in a cell envelope extract, was found to cross-react with monoclonal and polyclonal antibodies raised against vertebrate muscle myosin. Immunoelectron microscopy permitted precise localization of this polypeptide, indicating that myosin analogue was mainly distributed along the plasma membrane of resting and swollen conidia. In germinating conidia, indirect immunofluorescence microscopy revealed myosin analogue at the periphery of germ tubes, whereas actin appeared as dispersed punctate structures in the cytoplasm that were more concentrated at the site of germ tube emergence. A myosin ATPase inhibitor, butanedione monoxime, greatly reduced swelling and blocked germination. In contrast, when conidia were treated with cytochalasin B, an inhibitor of actin polymerization, swelling was not affected and germination was only partially reduced. Butanedione monoxime-treated conidia showed accumulation of cytoplasmic vesicles and did not achieve cell wall reorganization, unlike swollen conidia. Collectively, these results suggest an essential role for this myosin analogue in the deposition of cell wall components during germination of A. fumigatus conidia and therefore in host tissue colonization. (+info)
In vitro and in vivo activities of NS-718, a new lipid nanosphere incorporating amphotericin B, against Aspergillus fumigatus. (2/1653)We evaluated the in vitro and in vivo potencies of a new lipid nanosphere that incorporates amphotericin B (AmB), NS-718, against Aspergillus fumigatus. The in vitro activity of NS-718 (the MIC at which 90% of strains are inhibited [MIC90], 0.25 microgram/ml) against 18 isolates of A. fumigatus was similar to that of deoxycholate AmB (D-AmB; Fungizone; MIC90, 0.25 microgram/ml), but NS-718 was more potent than liposomal AmB (L-AmB; AmBi-some; MIC90, 1.0 microgram/ml). The in vivo efficacy of NS-718 in a rat model of invasive pulmonary aspergillosis was compared with those of D-AmB and L-AmB. A low dose (1 mg/kg of body weight) of L-AmB was ineffective (survival rate, 0%), although equivalent doses of D-AmB and NS-718 were more effective (survival rate, 17%). However, a higher dose of NS-718 (3 mg/kg) was more effective (survival rate, 100%) than equivalent doses of D-AmB and L-AmB (survival rate, 0%). To explain these differences, pharmacokinetic studies showed higher concentrations of AmB in the plasma of rats treated with NS-718 than in the plasma of those treated with D-AmB. Our results suggest that NS-718, a new preparation of AmB, is a promising antifungal agent with activity against pulmonary aspergillosis. (+info)
Amphotericin B- and fluconazole-resistant Candida spp., Aspergillus fumigatus, and other newly emerging pathogenic fungi are susceptible to basic antifungal peptides. (3/1653)The present study shows that a number of basic antifungal peptides, including human salivary histatin 5, a designed histatin analog designated dhvar4, and a peptide from frog skin, PGLa, are active against amphotericin B-resistant Candida albicans, Candida krusei, and Aspergillus fumigatus strains and against a fluconazole-resistant Candida glabrata isolate. (+info)
Early diagnosis of central nervous system aspergillosis with combination use of cerebral diffusion-weighted echo-planar magnetic resonance image and polymerase chain reaction of cerebrospinal fluid. (4/1653)We treated a patient diagnosed as central nervous system (CNS) aspergillosis with the combined use of cerebral diffusion-weighted echo-planar magnetic resonance imaging (DWI) and polymerase chain reaction of the cerebrospinal fluid (CSF-PCR). DWI, a cutting-edge imaging modality to reveal the earliest changes of cerebral infarction, detected cerebral fungal embolization when the conventional computed tomographic scan and magnetic resonance imaging failed to reveal it. CSF-PCR demonstrated the presence of Aspergillus-specific DNA in the specimen, when the conventional examination and culture of CSF were nonspecific or negative. These diagnostic methods could be useful in the early diagnosis of CNS aspergillosis. (+info)
Aspergillus meningitis: diagnosis by non-culture-based microbiological methods and management. (5/1653)The performance of antibody detection, antigen detection, and Aspergillus genus-specific PCR for diagnosing Aspergillus meningitis was investigated with 26 cerebrospinal fluid (CSF) samples obtained from a single patient with proven infection caused by Aspergillus fumigatus. Immunoglobulin G antibodies directed against Aspergillus were not detected by enzyme-linked immunosorbent assay in CSF or serum. The antigen galactomannan was detected in the CSF 45 days before a culture became positive, and Aspergillus DNA was detected 4 days prior to culture. Decline of the galactomannan antigen titer in the CSF during treatment with intravenous and intraventricular amphotericin B and intravenous voriconazole corresponded with the clinical response to treatment. (+info)
Contaminations occurring in fungal PCR assays. (6/1653)Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed. The identities of all contaminants were specified by cycle sequencing and GenBank analysis. Twelve of 150 PCR assays that together included over 2,800 samples were found to be contaminated (3.3% of the negative controls were contaminated during the DNA extraction, and 4.7% of the PCR mixtures were contaminated during the amplification process). Contaminants were specified as Aspergillus fumigatus, Saccharomyces cerevisiae, and Acremonium spp. Further analysis showed that commercially available products like zymolyase powder or 10x PCR buffer may contain fungal DNA. In conclusion, the risk of contamination is not higher in fungal PCR assays than in other diagnostic PCR-based assays if general precautions are taken. (+info)
Production of specific monoclonal antibodies to Aspergillus species and their use in immunohistochemical identification of aspergillosis. (7/1653)Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining. (+info)
Modulation of neutrophil-mediated activity against the pseudohyphal form of Candida albicans by granulocyte colony-stimulating factor (G-CSF) administered in vivo. (8/1653)Renewed interest in neutrophil transfusions has emerged with the development and clinical use of granulocyte colony-stimulating factor (G-CSF). G-CSF not only increases neutrophil (polymorphonuclear leukocyte, PMNL) production but also modulates various physiological properties of PMNL. The effects of G-CSF on PMNL-mediated fungicidal activity were evaluated by administration of G-CSF (300 micrograms/day subcutaneously) to 5 healthy volunteers for 6 days. G-CSF significantly enhanced PMNL-mediated damage of Candida albicans pseudohyphae by 33% (P=.007) on day 2 and by 44% (P=.04) on day 6 at a 10:1 effector:target ratio. In contrast, the ability of PMNL to induce damage of hyphae from either Fusarium solani or Aspergillus fumigatus did not significantly change during the study period. These data demonstrate that G-CSF administered in vivo modulates PMNL-mediated fungicidal activity against the pseudohyphal form of C. albicans, thereby suggesting potential utility of G-CSF as a biologic response-modifying therapy in some opportunistic fungal infections. (+info)
Bacterium induces cryptic meroterpenoid pathway in the pathogenic fungus Aspergillus fumigatus. - Leibniz-Institut für...
Stimulating encounter: The intimate, physical interaction between the soil-derived bacterium Streptomyces rapamycinicus and the human pathogenic fungus Aspergillus fumigatus led to the activation of an otherwise silent polyketide synthase (PKS) gene cluster coding for an unusual prenylated polyphenol (fumicycline A). The meroterpenoid pathway is regulated by a pathway-specific activator gene as well as by epigenetic factors.. ...
Characterization of Essential Genes by Parasexual Genetics in the Human Fungal Pathogen Aspergillus fumigatus: Impact of...
A. fumigatus strains and culture conditions: A. fumigatus strains used in this study are listed in Table 1. A. fumigatus strains were propagated at 37° on complete medium or minimal medium (MM) with 0.5 mm of one of the following nitrogen sources: sodium glutamate, ammonium tartrate, sodium nitrate, sodium nitrite, or hypoxanthine (Cove 1966). Uridine and uracil were added at a concentration of 5 mm when appropriate. Selection of A. fumigatus mutants unable to utilize nitrate as the sole nitrogen source was obtained by plating spores on MM containing ammonium tartrate and 100 mm sodium chlorate (Cove 1966). The nature of the mutation was assessed by growth on MM supplemented with different nitrogen sources, as previously described (Cove 1976). Selection of A. fumigatus mutants auxotrophic for uridine and uracil was achieved on MM containing 1 mg/ml 5-fluoroorotic acid plus uridine and uracil (dEnfert 1996). Liquid cultures used for DNA-mediated transformation and genomic DNA preparation were ...
Sphingolipids from the human fungal pathogen Aspergillus fumigatus. - Institut Pasteur
Sphingolipids (SPLs) are key components of the plasma membrane in yeast and filamentous fungi. These molecules are involved in a number of cellular processes, and particularly, SGLs are essential components of the highly polarized fungal growth where they are required for the formation of the polarisome organization at the hyphal apex. Aspergillus fumigatus, a human fungal pathogen, produce SGLs that are discriminated into neutral cerebrosides, glycosylinositolphosphoceramides (GIPCs) and glycosylphosphatidylinositol (GPI) anchors. In addition to complex hydrophilic head groups of GIPCs, A. fumigatus is, to date, the sole fungus that produces a GPI-anchored polysaccharide. These SPLs follow three different biosynthetic pathways. Genetics blockage leading to the inhibition of any SPL biosynthesis or to the alteration of the structure of SPL induces growth and virulence defects. The complete lipid moiety of SPLs is essential for the lipid microdomain organization and their biosynthetic pathways are
Virulence determinants of the human pathogenic fungus Aspergillus fumigatus protect against soil amoeba predation. - Leibniz...
Filamentous fungi represent classical examples for environmentally acquired human pathogens whose major virulence mechanisms are likely to have emerged long before the appearance of innate immune systems. In natural habitats, amoeba predation could impose a major selection pressure towards the acquisition of virulence attributes. To test this hypothesis, we exploited the amoeba Dicytostelium discoideum to study its interaction with Aspergillus fumigatus, two abundant soil inhabitants for which we found co-occurrence in various sites. Fungal conidia were efficiently taken up by D. discoideum, but ingestion was higher when conidia were devoid of the green fungal spore pigment DHN-melanin, in line with earlier results obtained for immune cells. Conidia were able to survive phagocytic processing and intracellular germination was initiated only after several hours of coincubation which eventually led to a lethal disruption of the host cell. Besides phagocytic interactions, both amoeba and fungus ...
MybA, a transcription factor involved in conidiation and conidial viability of the human pathogen Aspergillus fumigatus |...
Link to Pubmed [PMID] - 28677124. Mol. Microbiol. 2017 Sep;105(6):880-900. Aspergillus fumigatus, a ubiquitous human fungal pathogen, produces asexual spores (conidia), which are the main mode of propagation, survival and infection of this human pathogen. In this study, we present the molecular characterization of a novel regulator of conidiogenesis and conidial survival called MybA because the predicted protein contains a Myb DNA binding motif. Cellular localization of the MybA::Gfp fusion and immunoprecipitation of the MybA::Gfp or MybA::3xHa protein showed that MybA is localized to the nucleus. RNA sequencing data and a uidA reporter assay indicated that the MybA protein functions upstream of wetA, vosA and velB, the key regulators involved in conidial maturation. The deletion of mybA resulted in a very significant reduction in the number and viability of conidia. As a consequence, the ΔmybA strain has a reduced virulence in an experimental murine model of aspergillosis. RNA-sequencing and ...
Frontiers | Lipoxygenase Activity Accelerates Programmed Spore Germination in Aspergillus fumigatus | Microbiology
The opportunistic human pathogen Aspergillus fumigatus initiates invasive growth through a programmed germination process that progresses from dormant spore to swollen spore (SS) to germling (GL) and ultimately invasive hyphal growth. We find a lipoxygenase with considerable homology to human Alox5 and Alox15, LoxB, that impacts the transitions of programmed spore germination. Overexpression of loxB (OE::loxB) increases germination with rapid advance to the GL stage. However, deletion of loxB (ΔloxB) or its signal peptide only delays progression to the SS stage in the presence of arachidonic acid (AA); no delay is observed in minimal media. This delay is remediated by the addition of the oxygenated AA oxylipin 5-hydroxyeicosatetraenoic acid (5-HETE) that is a product of human Alox5. We propose that A. fumigatus acquisition of LoxB (found in few fungi) enhances germination rates in polyunsaturated fatty acid-rich environments.
Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus. | J. Craig Venter Institute
We present the genome sequences of a new clinical isolate of the important human pathogen, Aspergillus fumigatus, A1163, and two closely related but rarely pathogenic species, Neosartorya fischeri NRRL181 and Aspergillus clavatus NRRL1. Comparative genomic analysis of A1163 with the recently sequenced A. fumigatus isolate Af293 has identified core, variable and up to 2% unique genes in each genome. While the core genes are 99.8% identical at the nucleotide level, identity for variable genes can be as low 40%. The most divergent loci appear to contain heterokaryon incompatibility (het) genes associated with fungal programmed cell death such as developmental regulator rosA. Cross-species comparison has revealed that 8.5%, 13.5% and 12.6%, respectively, of A. fumigatus, N. fischeri and A. clavatus genes are species-specific. These genes are significantly smaller in size than core genes, contain fewer exons and exhibit a subtelomeric bias. Most of them cluster together in 13 chromosomal islands, ...
A Conserved C-Terminal Domain of the Aspergillus fumigatus Developmental Regulator MedA Is Required for Nuclear Localization,...
TY - JOUR. T1 - A Conserved C-Terminal Domain of the Aspergillus fumigatus Developmental Regulator MedA Is Required for Nuclear Localization, Adhesion and Virulence. AU - Al Abdallah, Qusai. AU - Choe, Se In. AU - Campoli, Paolo. AU - Baptista, Stefanie. AU - Gravelat, Fabrice N.. AU - Lee, Mark J.. AU - Sheppard, Donald C.. PY - 2012/11/21. Y1 - 2012/11/21. N2 - MedA is a developmental regulator that is conserved in the genome of most filamentous fungi. In the pathogenic fungus Aspergillus fumigatus MedA regulates conidiogenesis, adherence to host cells, and pathogenicity. The mechanism by which MedA governs these phenotypes remains unknown. Although the nuclear import of MedA orthologues has been reported in other fungi, no nuclear localization signal, DNA-binding domain or other conserved motifs have been identified within MedA. In this work, we performed a deletion analysis of MedA and identified a novel domain within the C-terminal region of the protein, designated MedA346-557, that is ...
Verruculogen associated with Aspergillus fumigatus hyphae and conidia modifies the electrophysiological properties of human...
The production of toxins by A. fumigatus may help the fungus to colonize and invade the respiratory epithelium by modifying the natural clearance of the respiratory tract. Previous research has shown that A. fumigatus culture filtrate modifies the transepithelial resistance (Rt) and transepithelial potential differences (Vt) of HNEC, an in vitro model of the air-liquid interface of airway epithelium . The aim of this study was to use HPLC and MS-MS to identify which toxins produced by A. fumigatus are responsible for these modifications. Our data suggest that verruculogen, which has never been implicated in invasive aspergillosis, is one of the probable candidates.. The fact that A. fumigatus produces a number of biologically active substances that slow ciliary beating, damage epithelium, and that may affect colonization of the airways has already been reported using culture explants . Among these substances, such toxins as gliotoxin, fumagillin, and helvolic acid have been implicated in ...
A new epimer of azaphilone derivative pinophilin B from the gorgonian-derived fungus Aspergillus fumigatus 14-27. - Fungal...
A new epimer of azaphilone derivative pinophilin B, epi-pinophilin B (1), and three known analogues (2-4) were obtained from the culture of the gorgonian-derived fungus Aspergillus fumigatus 14-27. The structures of 1-4, including their relative configurations were determined by extensive spectroscopic analysis and comparing with literature data. The absolute configuration of 1 was determined by electronic circular dichroism (ECD) and optical rotatory (OR) calculations methods. Compounds 1-4 were isolated from A. fumigatus for the first time. Their antibacterial and cytotoxic activities were also evaluated. PMID: 31564133 [PubMed - as supplied by publisher]. Source: Industry. ...
Microbiology Society Journals | Production of melanin by Aspergillus fumigatus
Melanins, or melanin-like compounds, may play a role in the pathogenesis of a number of human fungal infections. This study investigated the production of melanin by the important opportunistic pathogen Aspergillus fumigatus. Conidia from A. fumigatus were harvested and treated with proteolytic enzymes, denaturant and hot, concentrated acid; this yielded dark particles which were similar in size and shape to the original propagules. Electron spin resonance spectroscopy revealed that the conidial-derived particles were stable free radicals consistent with an identification as melanin. Melanin particles were used to immunize BALB/c mice in order to produce a total of five anti-melanin monoclonal antibodies (mAbs). The latter mAbs were strongly reactive both with intact conidia and with extracted melanin particles by ELISA and immunofluorescence reactivity. Immunofluorescence labelling with the novel mAbs was used to examine the temporal expression of melanin during in vitro culture of A. fumigatus
Biosynthesis of β-(1→5)-galactofuranosyl chains of fungal-type and o-mannose-type galactomannans within the invasive pathogen...
TY - JOUR. T1 - Biosynthesis of β-(1→5)-galactofuranosyl chains of fungal-type and o-mannose-type galactomannans within the invasive pathogen aspergillus fumigatus. AU - Chihara, Yuria. AU - Tanaka, Yutaka. AU - Izumi, Minoru. AU - Hagiwara, Daisuke. AU - Watanabe, Akira. AU - Takegawa, Kaoru. AU - Kamei, Katsuhiko. AU - Shibata, Nobuyuki. AU - Ohta, Kazuyoshi. AU - Oka, Takuji. PY - 2020/1/1. Y1 - 2020/1/1. N2 - ABSTRACT The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes. Galactomannans comprise α-(1→2)-/α-(1→6)-mannan and β-(1→5)-/ β-(1→6)-galactofuranosyl chains. We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains. In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus. Two paralogs exist within A. ...
Intratracheal exposure of rats to Aspergillus fumigatus spores isolated from sawmills in Sweden
Land, C.J.; Sostarić, B.; Fuchs, R.; Lundström, H.; Hult, K., 1989: Intratracheal exposure of rats to Aspergillus fumigatus spores isolated from sawmills in Sweden
Rapid Detection of Aspergillus Fumigatus Growth Percentage by Microplate Reader
In this experiment, the OD value of different concentrations of Aspergillus fumigatus cultured for 48 hours was measured on a microplate reader using a 96-well plate. The absorbance-concentration curve was plotted according to the results, and the absorbance value was used instead of visual observation to quickly detect the growth of Aspergillus fumigatus. situation. The results showed that the OD value was between 0.05 and 0.06 when visually 50%, and 100% when the OD value was greater than 0.17. The use of the OD value can be a rapid method for observing the growth of Aspergillus fumigatus, which has the advantages of high efficiency, accuracy and high throughput compared with the visual method.. ...
In Vitro and In Vivo Exposure-Effect Relationship of Liposomal Amphotericin B against Aspergillus fumigatus | Antimicrobial...
The pharmacodynamics (PD) of liposomal amphotericin B (L-AMB) remain relatively poorly understood because of complex pharmacokinetics (PK) that impede the in-depth comprehension of its exposure-response relationship (1). Although high L-AMB doses up to 15 mg/kg have been used (2), in the absence of a clinical dose-response relationship a dose of 3 mg/kg is generally recommended for the treatment of invasive aspergillosis (IA) (1), with an end-of-treatment favorable response of ∼40% for probable/proven cases (3). However, neutropenia may affect the clinical response to L-AMB therapy (4). We therefore studied L-AMB PD in an in vitro PK/PD model using previously published data of experimental aspergillosis in neutropenic and nonneutropenic animal models and optimized L-AMB therapy simulating human serum concentration-time profiles against azole-susceptible and azole-resistant Aspergillus fumigatus isolates in neutropenic and nonneutropenic patients.. Two clinical A. fumigatus isolates, a ...
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Glycoinositolphosphoceramides (GIPCs) are complex sphingolipids present at the plasma membrane of various eukaryotes with the important exception of mammals. In fungi, these glycosphingolipids commonly contain an alpha-mannose residue (Man) linked at position 2 of the inositol. However, several pathogenic fungi additionally synthesize zwitterionic GIPCs carrying an alpha-glucosamine residue (GlcN) at this position. In the human pathogen Aspergillus fumigatus, the GlcNalpha1,2IPC core (where IPC is inositolphosphoceramide) is elongated to Manalpha1,3Manalpha1,6GlcNalpha1,2IPC, which is the most abundant GIPC synthesized by this fungus. In this study, we identified an A. fumigatus N-acetylglucosaminyltransferase, named GntA, and demonstrate its involvement in the initiation of zwitterionic GIPC biosynthesis. Targeted deletion of the gene encoding GntA in A. fumigatus resulted in complete absence of zwitterionic GIPC; a phenotype that could be reverted by episomal expression of GntA in the mutant. ...
British Library EThOS: Early events in Aspergillus fumigatus infection : a chronicle of host-pathogen interactions
Host-pathogen interactions have critical implications for the establishment of disease and for determining adaptive immune responses of the host. This study has conducted global Aspergillus fumigatus transcriptional analyses throughout the initiation of murine infection using a wild-type and an attenuated ΔlaeA isolate. A novel data analysis protocol was applied from which three time-series datasets were generated between 4, 8 and 14 hours post infection. This approach identified distinct temporal gene expression profiles during disease initiation whereby numerous secreted enzymes, including proteases and antigens, were upregulated between 4 and 8 hours, while a striking upregulation of genes in secondary metabolism clusters and subtelomeric loci was observed between 8 and 14 hours. In order to test the role of several upregulated secondary metabolite genes on host-pathogen interactions and virulence, two isolates mutated in non-ribosomal peptide synthetase encoding genes (ΔftmA, Δpes3), and ...
Immune regulatory mechanisms and fungus-specific memory T cell responses - Vanessa Espinosa
The opportunistic fungal pathogen Aspergillus fumigatus is the causative agent of more than 90% of all Invasive Aspergillosis (IA) infections diagnosed in immun...
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Abstract Sulphur is an essential element that all pathogens have to absorb from their surroundings in order to grow inside their infected host. Despite its importance, the relevance of sulphur assimilation in fungal virulence is largely unexplored. Here we report a role of the bZIP transcription factor MetR in sulphur assimilation and virulence of the human pathogen Aspergillus fumigatus. The MetR regulator is essential for growth on a variety of sulphur sources; remarkably, it is fundamental for assimilation of inorganic S-sources but dispensable for utilization of methionine. Accordingly, it strongly supports expression of genes directly related to inorganic sulphur assimilation but not of genes connected to methionine metabolism. On a broader scale, MetR orchestrates the comprehensive transcriptional adaptation to sulphur-starving conditions as demonstrated by digital gene expression analysis. Surprisingly, A. fumigatus is able to utilize volatile sulphur compounds produced by its methionine ...
SREBP Coordinates Iron and Ergosterol Homeostasis to Mediate Triazole Drug and Hypoxia Responses in the Human Fungal Pathogen |...
1. KornitzerD 2009 Fungal mechanisms for host iron acquisition. Current opinion in microbiology 12 377 383. 2. SchrettlMBeckmannNVargaJHeinekampTJacobsenID 2010 HapX-mediated adaption to iron starvation is crucial for virulence of Aspergillus fumigatus. PLoS Pathog 6 e1001124 doi:10.1371/journal.ppat.1001124. 3. SchrettlMIbrahim-GranetODroinSHuerreMLatgeJP 2010 The crucial role of the Aspergillus fumigatus siderophore system in interaction with alveolar macrophages. Microbes and infection/Institut Pasteur 12 1035 1041. 4. SchrettlMBignellEKraglCSabihaYLossO 2007 Distinct roles for intra- and extracellular siderophores during Aspergillus fumigatus infection. PLoS Pathog 3 e128 doi:10.1371/journal.ppat.0030128. 5. SchrettlMBignellEKraglCJoechlCRogersT 2004 Siderophore biosynthesis but not reductive iron assimilation is essential for Aspergillus fumigatus virulence. The Journal of experimental medicine 200 1213 1219. 6. HissenAHChowJMPintoLJMooreMM 2004 Survival of Aspergillus fumigatus in serum ...
Contributions of both ATP-binding cassette transporter and Cyp51A proteins are essential for azole resistance in Aspergillus...
While azole drugs targeting the biosynthesis of ergosterol are effective antifungal agents, their extensive use has led to the development of resistant organisms. Infections involving azole resistant forms of the filamentous fungus Aspergillus fumigatus are often associated with genetic changes in the cyp51A gene encoding the lanosterol α14 demethylase target enzyme. Both a sequence duplication in the cyp51A promoter (TR34) as well as a substitution mutation in the coding sequence (L98H) are required for full expression of azole resistance. A mechanism commonly observed in pathogenic yeast such as Candida albicans involves gain-of-function mutations in transcriptional regulatory proteins that induce expression of ATP-binding cassette (ABC) transporter encoding genes. We and others have found that an ABC transporter protein called Cdr1B (here referred to as AbcG1) is required for wild-type azole resistance in A.fumigatus Here we test the genetic relationship between the TR34 L98H allele of ...
Aspergillus fumigatus Fresenius ATCC ® 1022D-2™
Aspergillus fumigatus ATCC ® 1022D-2™ Designation: Genomic DNA from Aspergillus fumigatus Strain 118 [ATCC ® 1022™] Application:
Secretome diversity and quantitative analysis of cellulolytic Aspergillus fumigatus Z5 in the presence of different carbon...
Aspergillus fumigatus Z5 has a strong ability to decompose lignocellulose biomass, and its extracellular protein secretion has been reported in earlier studies employing traditional techniques. However, a comprehensive analysis of its secretion in the presence of different carbon sources is still lacking. The goal of this work was to identify, quantify and compare the secretome of A. fumigatus Z5 in the presence of different carbon sources to understand in more details the mechanisms of lignocellulose decomposition by Aspergillus fumigatus Z5. Cellulolytic A. fumigatus Z5 was grown in the presence of glucose (Gl), Avicel (Av) and rice straw (RS), and the activities of several lignocellulosic enzymes were determined with chromatometry method. The maximum activities of endoglucanase, exoglucanase, β-glucosidase, laminarinase, lichenase, xylanase and pectin lyase were 12.52, 0.59, 2.30, 2.37, 1.68, 15.02 and 11.40 U·ml-1, respectively. A total of 152, 125 and 61 different proteins were identified in the
Targeting iron acquisition blocks infection with the fungal pathogens Aspergillus fumigatus and Fusarium oxysporum.
Filamentous fungi are an important cause of pulmonary and systemic morbidity and mortality, and also cause corneal blindness and visual impairment worldwide. Utilizing in vitro neutrophil killing assays and a model of fungal infection of the cornea, we demonstrated that Dectin-1 dependent IL-6 production regulates expression of iron chelators, heme and siderophore binding proteins and hepcidin in infected mice. In addition, we show that human neutrophils synthesize lipocalin-1, which sequesters fungal siderophores, and that topical lipocalin-1 or lactoferrin restricts fungal growth in vivo. Conversely, we show that exogenous iron or the xenosiderophore deferroxamine enhances fungal growth in infected mice. By examining mutant Aspergillus and Fusarium strains, we found that fungal transcriptional responses to low iron levels and extracellular siderophores are essential for fungal growth during infection. Further, we showed that targeting fungal iron acquisition or siderophore biosynthesis by ...
Siderophore Biosynthesis But Not Reductive Iron Assimilation Is Essential for Aspergillus fumigatus Virulence | JEM
Aspergillus fumigatus is the most common airborne fungal pathogen of humans, causing life-threatening invasive disease in immunocompromised patients. The limitations of therapeutic intervention are reflected in mortality rates, dependent primarily on the immune status of the host, of up to 90% (1, 2). The importance of the host immune status has been underlined by the lack of identified specific virulence attributes in A. fumigatus (1, 3-6). Limiting access to essential nutrients is an often overlooked aspect of innate immunity (7). Iron is essential for most organisms, suggesting that its acquisition in vivo may be required for A. fumigatus to cause disease (8-10). Iron uptake systems are often required for bacterial and yeast virulence (11, 12).. Fungi have evolved various strategies, often used in parallel, to acquire iron. These include two high affinity uptake mechanisms, reductive iron assimilation, and siderophore-assisted iron mobilization (8-10). Siderophores are low molecular mass, ...
PU.1 is involved in the immune response to Aspergillus fumigatus through upregulating Dectin-1 expression | BMC Infectious...
Invasive aspergillosis is a life-threatening disease, and its incidence has increased in the recent past. Dectin-1 recognizes β-glucans and mediates innate immune responses to Aspergillus fumigatus. Transcription factor PU.1 has been the focus of recent research due to its role in inflammation and infection. However, its role in Dectin-1 regulation during A. fumigatus infection remains to be elucidated. THP-1 cells were stimulated with A. fumigatus conidia. We then used real-time RT-PCR, Western blot, and immunofluorescence assays to analyze the mRNA and protein levels and cellular distribution, respectively, of Dectin-1 and PU.1 in stimulated THP-1 cells. Additionally, we used the luciferase reporter assays, chromatin immunoprecipitation (ChIP) assays, electrophoretic mobility shift assays (EMSA), and RNA interference experiments to investigate the role of PU.1 in Dectin-1 regulation. Our results revealed that Dectin-1 mRNA and protein levels as well as the PU.1 protein level were increased in THP-1
Frontiers | Revealing the Virulence Potential of Clinical and Environmental Aspergillus fumigatus Isolates Using Whole-Genome...
Aspergillus fumigatus is considered a common causative agent of human fungal infections. A restricted number of virulence factors have been described, and none of them lead to a differentiation in the virulence level among different strains. Variations in the virulence phenotype depending on the isolate origin, measured as survival percentage in animal infection models, have been previously reported. In this study, we analyzed the whole-genome sequence of A. fumigatus isolates from clinical and environmental origins to determine their virulence genetic content. The sample included four isolates sequenced at the University Medical Center Groningen (UMCG), three clinical (two of them isolated from the same patient) and the experimental strain B5233, and the draft genomes of one reference strain, two environmental and two clinical isolates obtained from a public database. The fungal genomes were screened for the presence of virulence-related genes (VRGs) using an in-house database of 244 genes related to
Full text] Sensitization to Aspergillus fumigatus as a risk factor for bronchiect | COPD
Sensitization to Aspergillus fumigatus as a risk factor for bronchiectasis in COPD Stephanie Everaerts,1,2 Katrien Lagrou,3,4 Adriana Dubbeldam,5 Natalie Lorent,1 Kristina Vermeersch,2 Erna Van Hoeyveld,3 Xavier Bossuyt,3,4 Lieven J Dupont,1,2 Bart M Vanaudenaerde,2 Wim Janssens1,2 1Department of Respiratory Diseases, University Hospitals Leuven, 2Laboratory of Respiratory Diseases, Department of Clinical and Experimental Medicine, KU Leuven, 3Department of Laboratory Medicine, University Hospitals Leuven, 4Department of Microbiology and Immunology, KU Leuven, 5Department of Radiology, University Hospitals Leuven, Leuven, Belgium Background: Bronchiectasisâ chronic obstructive pulmonary disease (COPD) overlap presents a possible clinical phenotype of COPD, but it is unclear why it develops in a subset of patients. We hypothesized that sensitization to Aspergillus fumigatus (A fum) is associated with bronchiectasis in COPD and occurs more frequently in vitamin D-deficient patients.Methods: This
Integrative analysis of the heat shock response in Aspergillus fumigatus | BMC Genomics | Full Text
Aspergillus fumigatus is a thermotolerant human-pathogenic mold and the most common cause of invasive aspergillosis (IA) in immunocompromised patients. Its predominance is based on several factors most of which are still unknown. The thermotolerance of A. fumigatus is one of the traits which have been assigned to pathogenicity. It allows the fungus to grow at temperatures up to and above that of a fevered human host. To elucidate the mechanisms of heat resistance, we analyzed the change of the A. fumigatus proteome during a temperature shift from 30°C to 48°C by 2D-fluorescence difference gel electrophoresis (DIGE). To improve 2D gel image analysis results, protein spot quantitation was optimized by missing value imputation and normalization. Differentially regulated proteins were compared to previously published transcriptome data of A. fumigatus. The study was augmented by bioinformatical analysis of transcription factor binding sites (TFBSs) in the promoter region of genes whose corresponding
Dirhamnolipids secreted from Pseudomonas aeruginosa modify anjpegungal susceptibility of Aspergillus fumigatus by inhibiting β1...
Pseudomonas aeruginosa and Aspergillus fumigatus are the two microorganisms responsible for most of the chronic infections in cystic fibrosis patients. P.
Aspergillus fumigatus protein phosphatase PpzA is involved in iron assimilation, secondary metabolite production, and virulence
Metal restriction imposed by mammalian hosts during an infection is a common mechanism of defence to reduce or avoid the pathogen infection. Metals are essential for organism survival due to its involvement in several biological processes. Aspergillus fumigatus causes invasive aspergillosis, a disea …
Serval - Heat shock protein 90 (Hsp90): A novel antifungal target against Aspergillus fumigatus.
Invasive aspergillosis is a life-threatening and difficult to treat infection in immunosuppressed patients. The efficacy of current anti-Aspergillus therapies, targeting the cell wall or membrane, is limited by toxicity (polyenes), fungistatic activity and some level of basal resistance (echinocandins), or the emergence of acquired resistance (triazoles). The heat shock protein 90 (Hsp90) is a conserved molecular chaperone involved in the rapid development of antifungal resistance in the yeast Candida albicans. Few studies have addressed its role in filamentous fungi such as Aspergillus fumigatus, in which mechanisms of resistance may differ substantially. Hsp90 is at the center of a complex network involving calcineurin, lysine deacetylases (KDAC) and other client proteins, which orchestrate compensatory repair mechanisms of the cell wall in response to the stress induced by antifungals. In A. fumigatus, Hsp90 is a trigger for resistance to high concentrations of caspofungin, known as
ASMscience | Aspergillus fumigatus a
Modern medical technologies are repairing the human body in ways never imagined only a few years ago, but they are leaving an increasing population of patients who are newly susceptible to opportunistic pathogens. Invasive and chronic fungal infections have become a formidable clinical opponent, and foremost among them is Aspergillus fumigatus. Aspergillusfumigatus and Aspergillosis assembles chapters from a large and international contingent of experts in the field to explore every major aspect of A. fumigatus and how it kills so many patients. This volume offers the latest insights into the fundamental biology and pathogenesis of A. fumigatus and how it establishes disease, as well as the newest strategies for characterizing, diagnosing, and treating its spectrum of clinical infection. This valuable book is an instrumental resource for both scientists and clinicians tackling the current problems with Aspergillosis. It presents chapters on the species itself, including morphology and unique and
Structure of Aspergillus fumigatus calcineurin-FK506-FKBP12 complex reveals critical FKBP12-binding residues distinct from...
Purpose: Calcineurin orchestrates growth, stress responses and virulence in major pathogenic fungi including Aspergillus fumigatus responsible for life-threatening fungal infections worldwide. While these cellular regulatory functions of calcineurin make it an attractive antifungal target, the immunosuppressive effects of the currently available calcineurin inhibitors, FK506 and CsA, make it difficult to exploit the antifungal potential due to conservation of calcineurin in the host and the fungal pathogen. Critical molecular understanding of calcineurin-immunophilin-immunosuppressor complexes would facilitate the design of novel non-immunosuppressive CsA and FK506 analogs for fungal-specific targeting of calcineurin.. Methods: We solved the crystal structure of calcineurin-FK506-FKBP12 complex in A. fumigatus and using site-directed mutagenic approaches, we constructed several mutations in the CnaA catalytic subunit of calcineurin and FKBP12. To identify differences between the A. fumigatus ...
Characterization of Immune Responses to Repeated Aspergillus fumigatus by Amanda Dawn Buskirk
Personal exposures to A. fumigatus are associated with a variety of adverse health outcomes, including invasive aspergillosis, allergic sensitization, and asthma. Due to the high rate of mortality associated with invasive disease in immunocompromised patients, most studies of A. fumigatus have aimed to characterize the immune responses in immunocompromised murine models. However, a larger portion of the population is affected by fungal-induced allergies and asthma and the immune mechanisms associated with exposure have rarely been studied in an immunocompetent model. These models do not accurately reflect the natural method of exposure to environmental sources of conidia, and may significantly impact responses between fungi and the host immune system. Furthermore, little is known about the mechanisms associated with fungal induced allergy and asthma. Persistence of antigen is believed to play a role in induction of these diseases. Melanin, an A. fumigatus virulence factor, protects conidia from innate
Dsc Orthologs Are Required for Hypoxia Adaptation, Triazole Drug Responses, and Fungal Virulence in Aspergillus fumigatus |...
The SREBP SrbA in A. fumigatus has previously been shown to be required for hypoxia and low-iron adaptation, triazole drug susceptibility, and virulence (4, 5, 47). Given the potential importance of SrbA in fungal virulence and responses to antifungal therapy, a further examination of the mechanisms of SrbA regulation and function is warranted in A. fumigatus. SREBPs exist as precursor proteins that are cleaved to release the active N terminus transcription factor (8). In A. fumigatus, whether SrbA required proteolytic processing for function as a transcription factor was unclear. Bioinformatic analyses of the SrbA amino acid sequence strongly suggest that SrbA is an integral membrane protein like SREBPs in other organisms. Here, we observed that the GFP signal from an SrbA:GFP fusion protein strain largely localizes to the nuclear envelope/ER membrane, supporting the bioinformatics data with regard to SrbA localization in A. fumigatus. However, previous attempts to identify the sterol cleavage ...
Aspergillus fumigatus and Aspergillosis by Jean-Paul Latgé, William J. Steinbach - Jason Books
2005. The Aspergillus nidulans phytochrome FphA represses sexual development in red light. Curr. Biol. 15:1833-1838. Casselton, L. A. 2002. Mate recognition in fungi. Heredity 88:142- 147. , J. P. Debeaupuis, J. Sarfati, J. Lortholary, P. Ribaud, P. Shah, M. Cornet, H. V. Thien, E. Gluckman, G. Bru ¨cker, and J. P. Latge´. 1998. Molecular typing of environmental and patient isolates of Aspergillus fumigatus from various hospital settings. J. Clin. Microbiol. 36:1494-1500. Debeaupuis, J. , J. Sarfati, V. Momany, T. Tanaka, T. Kumagai, K. Asai, M. Machida, W. C. Nierman, D. W. Denning, M. Caddick, M. Hynes, M. Paoletti, R. Fischer, B. Miller, P. Dyer, M. S. Sachs, S. A. Osmani, and B. W. Birren. 2005. Sequencing and comparative analysis of Aspergillus nidulans. Nature 438:1105-1115. , M. Christensen, A. H. Onions, J. I. Pitt, and R. A. Samson. 1985. Infrageneric taxa of Aspergillus, p. 55-62. In R. A. Samson and J. I. ), Advances in Penicillium and Aspergillus Systematics. Plenum Press, New ...
Bioluminescent Aspergillus fumigatus, a new tool for drug efficiency testing and in vivo monitoring of invasive aspergillosis.
Aspergillus fumigatus is the main cause of invasive aspergillosis in immunocompromised patients, and only a limited number of drugs for treatment are available. A screening method for new antifungal compounds is urgently required, preferably an appro
Bola fúngica por Aspergillus fumigatus em cavidade pleural
Objective: To report the cases of 6 patients with fungus ball caused by Aspergillus fumigatus (aspergilloma) in the pleural cavity. Methods: Between 1980 and 2009, 391 patients were diagnosed with aspergilloma at the Santa Casa Hospital Complex in Porto Alegre, Brazil. The diagnosis of aspergilloma in the pleural cavity was made through imaging tests revealing effusion and pleural thickening with air-fluid level; direct mycological examination revealing septate hyphae, consistent with Aspergillus sp.; and positive culture for A. fumigatus in the surgical specimen from the pleural cavity. Results: Of the 391 patients studied, 6 (2%) met the established diagnostic criteria. The mean age of those 6 patients was 48 years (range, 29-66 years), and 5 (83%) were male. The most common complaints were cough, expectoration, and hemoptysis. Four patients (67%) had a history of tuberculosis that had been clinically cured. All of the patients were submitted to surgical removal of the aspergilloma, followed ...
Hyperspectral Imaging Using Intracellular Spies: Quantitative Real-Time Measurement of Intracellular Parameters In Vivo during...
Hyperspectral imaging (HSI) is a technique based on the combination of classical spectroscopy and conventional digital image processing. It is also well suited for the biological assays and quantitative real-time analysis since it provides spectral and spatial data of samples. The method grants detailed information about a sample by recording the entire spectrum in each pixel of the whole image. We applied HSI to quantify the constituent pH variation in a single infected apoptotic monocyte as a model system. Previously, we showed that the human-pathogenic fungus Aspergillus fumigatus conidia interfere with the acidification of phagolysosomes. Here, we extended this finding to monocytes and gained a more detailed analysis of this process. Our data indicate that melanised A. fumigatus conidia have the ability to interfere with apoptosis in human monocytes as they enable the apoptotic cell to recover from mitochondrial acidification and to continue with the cell cycle. We also showed that this ability of A
The putative polysaccharide synthase AfCps1 regulates Aspergillus fumigatus morphogenesis and conidia immune response in mouse...
Aspergillus fumigatus is a well-known opportunistic pathogen that causes invasive aspergillosis (IA) infections with high mortality in immunosuppressed individuals. Morphogenesis, including hyphal growth, conidiation, and cell wall biosynthesis is crucial in A. fumigatus pathogenesis. Based on a previous random insertional mutagenesis library, we identified the putative polysaccharide synthase gene Afcps1 and its para-log Afcps2. Homologs of the cps gene are commonly found in the genomes of most fungal and some bacterial pathogens. Afcps1/cpsA is important in sporulation, cell wall composition, and virulence. However, the precise regulation patterns of cell wall integrity by Afcps1/cpsA and further effects on the immune response are poorly understood. Specifically, our in-depth study revealed that Afcps1 affects cell-wall stability, showing an increased resistance of ΔAfcps1 to the chitinmicrofibril destabilizing compound calcofluor white (CFW) and susceptibility of ΔAfcps1 to the ...
PACCARB 13th Public Mtg, Day 1 Pt 4: PANEL 2: Emerging Resistant Fungi - Aspergillus fumigatus - Tierra de Campeones
could get genotypically -,,Jacques Meis: Yes.,,Christine Ginocchio: - with the resistance. Okay.,,Jacques Meis: At least in Europe. Im not sure what the epidemiology is here. Probably, you have also a lot of -,,Tom Chiller: We dont - we dont ...
Aspergillus fumigatus | Mould Compendium | INSPQ
Aspergillus fumigatus Basics Laboratory Metabolites Adverse health reactions Specific settings Diagnostic Bibliography Basics A. fumigatus has been associated with each and every type of health problems linked to environmental moulds: irritation and inflammation, allergy, asthma, pneumonitis, toxic effects as well as a wide range of infections. Infections have been reported
The role of Syk signaling in antifungal innate immunity of human corneal epithelial cells | BMC Ophthalmology | Full Text
Fungal keratitis is a kind of intractable and sight-threatening diseases. Spleen-tyrosine kinase (Syk) is a non-receptor tyrosine kinase, which plays an important role in the signaling pathway of the receptors. In the current study, we investigate the expression and function of Syk in human corneal epithelial cells with Aspergillus fumigatus (A. fumigatus) infection. Cultured telomerase-immortalized human corneal epithelial cells (THCEs) were treated with A. fumigatus hyphae with or without treatment of Syk inhibitors. Activation of Syk and the role of Syk in regulating inflammatory cytokines and chemokines expression were evaluated. The mRNA expression was determined by real time PCR, and protein activation was measured by western blotting. Syk protein was detected in THCEs, and its activation was enhanced after treatment of A. fumigatus hyphae. Expression of inflammatory cytokines (IL-1β and IL-6) and chemokines (IL-8 and CXCL1) mRNA were significantly increased after stimulation of A. fumigatus
Characteristics of azole-resistant Aspergillus fumigatus attached to agricultural products imported to Japan. - Fungal...
Due to the increase in the number of azole-resistant Aspergillus fumigatus, there is an urgent need of data to predict future trends and prevent further spreading. The intercountry transfer of resistant A. fumigatus on plant bulbs have been reported. We investigated existence and characteristics of resistant isolates attached to agricultural products imported to Japan. We purchased 292 samples in Japan. All samples were screened for the existence of azole-resistant A. fumigatus. For positive isolates, minimum inhibitory concentrations of the drugs were determined. We also analyzed Cyp51A, Hmg1, and Erg6 mutations of these isolates and conducted microsatellite genotyping. Fourteen azole-resistant isolates were detected, of which 13 were cultured from flower bulbs imported from the Netherlands. Among them 5 were from 11 bulbs of Hippeastrum (45.5%), 5 were from 24 bulbs of Gladiolus (20.8%), 2 were from 4 bulbs of Ixia (50.0%), and 1 was from 22 bulbs of Tulipa (4.5%). Only 1 resistant isolate was ...
Detection of TR 34 /L98H CYP51A Mutation through Passive Surveillance for Azole-Resistant Aspergillus fumigatus in the United...
The emergence of azole-resistant Aspergillus fumigatus has become a clinical problem in many parts of the world. Several amino acid mutations in the azole target protein Cyp51Ap contribute to this resistance, with the most concerning being the environmentally derived TR34 /L98H and TR46 /Y121F/T289A mutations. Here, we performed passive surveillance to assess a sample of the A. fumigatus population in the United States for the presence of these mutations. We found 1.4% of those isolates to exhibit elevated MIC via broth microdilution, and five of those isolates harbored the TR34 /L98H mutation ...
Treatments to fight fungal infections which cause allergic bronchopulmonary aspergillosis in people with cystic fibrosis |...
Review question We planned to review evidence about the effect of treatments to fight fungal infections which cause allergic bronchopulmonary aspergillosis in people with cystic fibrosis.. Background Allergic bronchopulmonary aspergillosis (ABPA) is an allergic lung reaction to a type of fungus (usually Aspergillus fumigatus) in some people with cystic fibrosis. It causes cough and wheezing and sometimes fever. If left untreated ABPA can lead to chronic lung damage. It is usually treated with a high dose of corticosteroids (also known as steroids). However, it has not been proven that corticosteroids can prevent lung function deteriorating in the long term. Also, long-term use of steroids is linked to some serious side effects. Treating the fungus which causes ABPA may be an alternative to using high doses of steroids to combat the allergic reaction. This is an update of a previously published review.. Search date The evidence is current to: 29 September 2016.. Study characteristics No trials ...
Allergic Bronchopulmonary Aspergillosis (ABPA) | SYMPTOMA.com
Allergic Bronchopulmonary Aspergillosis (ABPA): Symptoms ❗ Workup ❗ Diagnosis ❗ Treatment ❗ Complications ❗ Causes ❗ Epidemiology ❗ Incidence ❗ Prognosis ❗ Check at SYMPTOMA.com Allergic bronchopulmonary aspergillosis is a disease characterized by a hypersensitivity reaction to aspergillus fumigatus after its repeated inhalation and is most…
AID 322857 - Antifungal activity against azole-resistant Aspergillus fumigatus V34/75-CM3276 isolate from patient with hyper...
BioAssay record AID 322857 submitted by ChEMBL: Antifungal activity against azole-resistant Aspergillus fumigatus V34/75-CM3276 isolate from patient with hyper-immunoglobulin E syndrome by broth microdilution susceptibility test.
Damage to Aspergillus fumigatus and Rhizopus oryzae hyphae by oxidative and nonoxidative microbicidal products of human...
TY - JOUR. T1 - Damage to Aspergillus fumigatus and Rhizopus oryzae hyphae by oxidative and nonoxidative microbicidal products of human neutrophils in vitro. AU - Diamond, R. D.. AU - Clark, R. A.. PY - 1982. Y1 - 1982. N2 - Our previous studies established that human neutrophils could damage and probably kill hyphae of Aspergillus fumigatus and Rhizopus oryzae in vitro, primarily by oxygen-dependent mechanisms active at the cell surface. These studies were extended, again quantitating hyphal damage by reduction in uptake of 14C-labeled uracil or glutamine. Neither A. fumigatus nor R. oryzae hyphae were damaged by neutrophils from patients with chronic granulomatous disease, confirming the importance of oxidative mechanisms in damage to hyphae. In contrast, neutrophils from one patient with hereditary myeloperoxidase deficiency damaged R.C. oryzae but not A. fumigatus hyphae. Cell-free, in vitro systems were then used to help determine the relative importance of several potentially fungicidal ...
Exposure of Aspergillus fumigatus to caspofungin results in the release, and de novo biosynthesis, of gliotoxin - Maynooth...
Caspofungin is a member of the echinocandin class of antifungal agents that inhibit the synthesis of β 1,3 glucan thus disrupting fungal cell wall structure and function. Exposure of the Aspergillus fumigatus cultures to caspofungin (0.01, 0.1 or 1.0 μ g/ml) resulted in a reduction in cell growth, but the production of the epipolythiodioxopiperazine toxin, gliotoxin, was comparable, or greater, in cultures exposed to caspofungin than untreated controls. Exposure of A. fumigatus hyphae to 1.0 μ g/ml caspofungin for 4 h resulted in the release of amino acids ( P 0.01), protein ( P 0.002) and gliotoxin ( P 0.02). Cultures of A. fumigatus incubated in the presence of caspofungin for 4 or 24 h demonstrated enhanced gliotoxin release ( P 0.04 and 0.03, respectively) and biosynthesis ( P 0.04 and 0.03, respectively) compared to that by control cultures. The results presented here indicate that exposure of A. fumigatus to caspofungin results in increased cell permeability and an increase in the ...
Voriconazole Versus Oral Steroids in Allergic Bronchopulmonary Aspergillosis - Full Text View - ClinicalTrials.gov
Allergic bronchopulmonary aspergillosis (ABPA) is a pulmonary disorder caused by a complex hypersensitivity response to antigens released by the fungus Aspergillus fumigatus. The management of ABPA includes two important aspects - institution of immunosuppressive therapy in the form of glucocorticoids to control the immunologic activity, and close monitoring for detection of relapses. Another possible target is to use antifungal agents to attenuate the fungal burden secondary to the fungal colonization in the airways. Oral corticosteroids are currently the treatment of choice for ABPA associated with bronchial asthma.They not only suppress the immune hyperfunction but are also anti-inflammatory. However, there is no data to guide the dose and duration of glucocorticoids and different regimens of glucocorticoids have been used in literature.Itraconazole, an oral triazole with relatively low toxicity, is active against Aspergillus spp. in vitro and in vivo. The activity of itraconazole against ...
Allergic bronchopulmonary aspergillosis treated successfully for one year with omalizumab<...
TY - JOUR. T1 - Allergic bronchopulmonary aspergillosis treated successfully for one year with omalizumab. AU - Collins, Jennifer. AU - de Vos, Gabriele. AU - Hudes, Golda. AU - Rosenstreich, David. PY - 2012. Y1 - 2012. N2 - Background: Current therapy for allergic bronchopulmonary aspergillosis (ABPA) uses oral corticosteroids, exposing patients to the adverse effects of these agents. There are reports of the steroid-sparing effect of anti-IgE therapy with omalizumab for ABPA in patients with cystic fibrosis (CF), but there is little information on its efficacy against ABPA in patients with bronchial asthma without CF. Objective: To examine the effects of omalizumab, measured by asthma control, blood eosinophilia, total serum immunoglobulin E (IgE), oral corticosteroid requirements, and forced expiratory volume spirometry in patients with ABPA and bronchial asthma. Methods: A retrospective review of charts from 2004-2006 of patients treated with omalizumab at an academic allergy and immunology ...
Aspergillus fumigatus infection in an ostrich with an enlarged neck due to respiratory problems -Korean Journal of...
This study describes a sudden death in an ostrich (Struthio camelus) with a big neck. Grossly, the air sacs were thickened. Yellow to white, round or coalescent material was scattered on the air sacs. However, the cervical air sac was normal. Histopathologically, multinucleated giant cells, heterophils, and macrophages had infiltrated the air sacs, and many hyphae were seen in the air sacs and on their surfaces. Pyogranulomatous inflammation with intralesional hyphae was observed throughout the lungs. Aspergillus fumigatus was identified microbiologically. In conclusion, the affected animal died because of the respiratory disturbance caused by mycotic airsacculitis and pneumonia.
Studies of Pseudomonas aeruginosa Mutants Indicate Pyoverdine as the Central Factor in Inhibition of Aspergillus fumigatus...
Pseudomonas aeruginosa and Aspergillus fumigatus are common opportunistic bacterial and fungal pathogens, respectively. They often coexist in airways of immunocompromised patients and individuals with cystic fibrosis, where they form biofilms and cause acute and chronic illnesses. Hence, the interactions between them have long been of interest and it is known that P. aeruginosa can inhibit A. fumigatusin vitro We have approached the definition of the inhibitory P. aeruginosa molecules by studying 24 P. aeruginosa mutants with various virulence genes deleted for the ability to inhibit A. fumigatus biofilms. The ability of P. aeruginosa cells or their extracellular products produced during planktonic or biofilm growth to affect A. fumigatus biofilm metabolism or planktonic A. fumigatus growth was studied in agar and liquid assays using conidia or hyphae. Four mutants, the pvdD pchE, pvdD, lasR rhlR, and lasR mutants, were shown to be defective in various assays. This suggested the P. aeruginosa ...
Allergic bronchopulmonary aspergillosis - Wikipedia
Allergic bronchopulmonary aspergillosis (ABPA) is a condition characterised by an exaggerated response of the immune system (a hypersensitivity response) to the fungus Aspergillus (most commonly Aspergillus fumigatus). It occurs most often in patients with asthma or cystic fibrosis. Aspergillus spores are ubiquitous in soil and are commonly found in the sputum of healthy individuals. A. fumigatus is responsible for a spectrum of lung diseases known as aspergilloses. ABPA causes airway inflammation, leading to bronchiectasis-a condition marked by abnormal dilation of the airways. Left untreated, the immune system and fungal spores can damage sensitive lung tissues and lead to scarring. The exact criteria for the diagnosis of ABPA are not agreed upon. Chest X-rays and CT scans, raised blood levels of IgE and eosinophils, immunological tests for Aspergillus together with sputum staining and sputum cultures can be useful. Treatment consists of corticosteroids and antifungal medications. Almost all ...
Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state...
Direct utilization of untreated oil palm trunk (OPT) for cellulases and xylanase production by Aspergillus fumigatus SK1 was conducted under solid-state fermentation (SSF). The highest activities of extracellular cellulases and xylanases were produced at 80% moisture level, initial pH 5.0, 1 × 108 spore/g (inoculum) with 125 μm of OPT as sole carbon source. The cellulases and xylanase activities obtained were 54.27, 3.36, 4.54 and 418.70 U/g substrates for endoglucanase (CMCase), exoglucanase (FPase), β-glucosidase and xylanase respectively. The crude cellulases and xylanase required acidic condition to retain their optimum activities (pH 4.0). Crude cellulases and xylanase were more stable at 40°C compared to their optimum activities conditions (60°C for FPase and 70°C for CMCase, β-glucosidase and xylanase). SDS-PAGE and zymogram analysis showed that Aspergillus fumigatus SK1 could secrete cellulases (endoglucanase, exoglucanase and β-glucosidase), xylanase and protease. Enzymatic ...
In Vitro and In Vivo Exposure-Effect Relationship of Liposomal Amphotericin B against Aspergillus fumigatus | Antimicrobial...
In vitro pharmacokinetic/pharmacodynamic data of liposomal amphotericin B (L-AMB) were compared with animal data from neutropenic and nonneutropenic models of azole-susceptible and azole-resistant invasive aspergillosis. L-AMB was equally effective. The in vitro fCmax (maximum concentration of free drug)/MIC ratio associated with 50% of maximal activity was 0.31 (0.29 to 0.33), similar to that in neutropenic but not nonneutropenic mice (0.11 [0.06 to 0.20]). Simulation analysis indicated that standard L-AMB doses (1 to 3 mg/kg) are adequate for nonneutropenic patients, but higher doses (7.5 to 10 mg/kg) may be required for neutropenic patients for Aspergillus fumigatus isolates with MICs of 0.5 to 1 mg/liter. ...
Enhanced IgE allergic response to Aspergillus fumigatus in CFTR-/- mic by Christian Mueller, Sofia A. Braag et al.
To gain insight into aberrant cytokine regulation in cystic fibrosis (CF), we compared the phenotypic manifestations of allergen challenge in gut-corrected CFTR-deficient mice with background-matched C57Bl6 (B6) mice. Aspergillus fumigatus (Af) antigen was used to mimic allergic bronchopulmonary aspergillosis, a peculiar hyper-IgE syndrome with a high prevalence in CF patients. CFTR-/-, C57BL/6 and FVB/NJ mice were sensitized with Af antigen by serial intraperitoneal injections. Control mice were mock sensitized with PBS. Challenges were performed by inhalation of Af antigen aerosol. After Af antigen challenge, histologic analysis showed goblet cell hyperplasia and lymphocytic infiltration in both strains. However, total serum IgE levels were markedly elevated in CF mice. Sensitized CF mice showed a five-fold greater IgE response to sensitization as compared with B6- and FVB-sensitized controls. Additional littermate controls to fully normalize for B6-FVB admixture in the strain background confirmed the
ASMscience | Invasive Aspergillosis i
Invasive mycoses represent a major cause of morbidity and mortality in patients with malignancy or undergoing hematopoietic stem cell transplantation (HSCT). Recently it has been shown that the Toll-like receptor system could play an important role in the development of invasive aspergillosis in patients receiving chemotherapy or after HSCT, and there is evidence suggesting that the involvement of Toll-like receptors during Aspergillus fumigatus infection is influenced by the immunological status of the host. The upper and lower respiratory tracts represent the most frequent locations of A. fumigatus infection in all patient categories and age groups. Among echinocandins, caspofungin has been approved for salvage therapy in patients with invasive aspergillosis, since it has been demonstrated to be well-tolerated and with a 39% response in patients with refractory infections. However, in this study as in other salvage therapy trials, the need for salvage therapy was established in the presence of
A Novel Environmental Azole Resistance Mutation in Aspergillus fumigatus and a Possible Role of Sexual Reproduction in Its...
This study investigated the dynamics of Aspergillus fumigatus azole-resistant phenotypes in two compost heaps with contrasting azole exposures: azole free and azole exposed. After heat shock, to which sexual but not asexual spores are highly resistant, the azole-free compost yielded 98% (49/50) wild-type and 2% (1/50) azole-resistant isolates, whereas the azole-containing compost yielded 9% (4/45) wild-type and 91% (41/45) resistant isolates. From the latter compost, 80% (36/45) of the isolates contained the TR46/Y121F/T289A genotype, 2% (1/45) harbored the TR46/Y121F/M172I/T289A/G448S genotype, and 9% (4/45) had a novel pan-triazole-resistant mutation (TR463/Y121F/M172I/T289A/G448S) with a triple 46-bp promoter repeat. Subsequent screening of a representative set of clinical A. fumigatus isolates showed that the novel TR463 mutant was already present in samples from three Dutch medical centers collected since 2012. Furthermore, a second new resistance mutation was found in this set that ...
Allergic Bronchopulmonary Aspergillosis (ABPA) Symptoms, Diagnosis, Treatment & Management | AAAAI
An overview of Allergic Bronchopulmonary Aspergillosis (ABPA) symptoms, diagnosis, treatment & management written by experts in allergy, asthma & immunology.
JoF | Free Full-Text | Allergic Bronchopulmonary Aspergillosis
Allergic bronchopulmonary aspergillosis (ABPA), a progressive fungal allergic lung disease, is a common complication of asthma or cystic fibrosis. Although ABPA has been recognized since the 1950s, recent research has underscored the importance of Th2 immune deviation and granulocyte activation in its pathogenesis. There is also strong evidence of widespread under-diagnosis due to the complexity and lack of standardization of diagnostic criteria. Treatment has long focused on downregulation of the inflammatory response with prolonged courses of oral glucocorticosteroids, but more recently concerns with steroid toxicity and availability of new treatment modalities has led to trials of oral azoles, inhaled amphotericin, pulse intravenous steroids, and subcutaneously-injected anti-IgE monoclonal antibody omalizumab, all of which show evidence of efficacy and reduced toxicity.
The Journal of Invasive Fungal Infections - Volume 4 Issue 2 - Antifungal Immunity
Chaudhary N, Staab JF, Marr KA. PLoS One 2010;5:e9036.. Aspergillus allergens are described as proteins that are recognized in patients with hypersensitivity syndromes such as allergic bronchopulmonary aspergillosis and Aspergillus-induced asthma. However, findings from this study indicate that (at least) some of these proteins are not only allergens, but are also capable of inducing a T-helper 1 (Th1) cytokine response in volunteers without a history of suspected or proven fungal infection or reported allergy or atopy. This indicates that these Asp f proteins are able to induce both protective (Th1) and non-protective (Th2) inflammation. Depending on the hosts status, Aspergillus fumigatus is able to cause several diseases ranging from allergic (e.g. Aspergillus-induced asthma and allergic bronchopulmonary aspergillosis) to invasive (e.g. pulmonary aspergillosis and disseminated disease). A fumigatus conidia enter the body via the air and, if they are not cleared by phagocytic cells, spores ...
Allergic Bronchopulmonary Aspergillosis - Lung and Airway Disorders - Merck Manuals Consumer Version
Allergic Bronchopulmonary Aspergillosis - Learn about the causes, symptoms, diagnosis & treatment from the Merck Manuals - Medical Consumer Version.
Allergic bronchopulmonary aspergillosis | Genetic and Rare Diseases Information Center (GARD) - an NCATS Program
A collection of disease information resources and questions answered by our Genetic and Rare Diseases Information Specialists for Allergic bronchopulmonary aspergillosis
Fatal mycotic encephalitis in a Northern bottlenose whale (Hyperoodon ampullatus) caused by Aspergillus fumigatus<...
TY - JOUR. T1 - Fatal mycotic encephalitis in a Northern bottlenose whale (Hyperoodon ampullatus) caused by Aspergillus fumigatus. AU - Foster, G. AU - Dagleish, Mark P. AU - Reid, RJ. AU - Barley, Jason. AU - Howie, F. PY - 2008/11/30. Y1 - 2008/11/30. M3 - Article. VL - 163. SP - 602. EP - 604. JO - Veterinary Record. JF - Veterinary Record. SN - 0042-4900. IS - 20. ER - ...
Page 3 - Renal and Urology News
Overview: What every practitioner needs to know Are you sure your patient has allergic bronchopulmonary aspergillosis? What are the typical findings for this disease? Allergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity lung disease due to bronchial colonization by Aspergillus fumigatus that occurs in susceptible patients with asthma and cystic fibrosis (CF). ABPA affects approximately 1-2%…. ...
Page 3 - Infectious Disease Advisor
Overview: What every practitioner needs to know Are you sure your patient has allergic bronchopulmonary aspergillosis? What are the typical findings for this disease? Allergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity lung disease due to bronchial colonization by Aspergillus fumigatus that occurs in susceptible patients with asthma and cystic fibrosis (CF). ABPA affects approximately 1-2%…. ...
Al harboured the same genotype of multi-triazole resistant A. fumigatus, isolated | http://amparinhibitor.com
Al harboured the same genotype of multi-triazole resistant A. fumigatus, isolated on two different occasions which raises concern on the exposure of hospitalized patients to this resistant genotype. In this context it is pertinent to mention that previously multi-triazole resistant TR34/L98H A. KS 176 biological activity fumigatus isolates have been reported from patients attending the outpatient departments of VPCI who were never exposed to azoles . In addition multi-triazole resistant A. fumigatus has also been isolated from admitted patients of VPCI. The presence of A. fumigatus resistant to medical triazoles poses a threat to immunocompromised patients as alternative therapy is limited. Snelders et al. reported that TR34/L98H isolates from clinical and environmental origins were cross resistant to five triazole DMIs fungicides, propiconazole, bromuconazole, tebuconazole, epoxiconazole and difenoconazole and thus supporting the hypothesis that exposure of A. fumigatus to azole fungicides ...
Eosinophilic Respiratory Problems: Allergic Bronchopulmonary Aspergillosis Paul A. Greenberger, M.D. 6 December 2011 Workshop...
Minimal Diagnostic Criteria for ABPA-Central Bronchiectasis (ABPA-CB) Asthma Central bronchiectasis Immediate cutaneous reactivity to Aspergillus species or A. fumigatus Elevated total serum IgE (>417 kU/L) Elevated serum IgE-A.fumigatus and or IgG- A.fumigatus compared to sera from prick positive patients with asthma
Most recent papers with the keyword Aspergillus fumigatus | Read by QxMD
Non-fumigatus Aspergillus species are the leading cause of Aspergillus infections in the tropical and subtropical regions of the world. In a prospective study between 2015 and 2016, a total of 150 bronchoalveolar (BAL) specimens was collected from patients suspected to pulmonary aspergillosis (PA) underlying immunodeficiencies in Mashhad, Northeastern Iran, located in the Middle East. All Aspergillus strains were phylogenetically identified at the species level by PCR-sequencing of partial β-tubulin gene. Overall, Aspergillus species were isolated from 20 specimens originating from 10 (50%) patients with cancer, 5 (25%) patients receiving corticosteroid therapy, 3 (15%) organ transplant recipients and 2 (10%) patients admitted to intensive care unit (ICU ...
Prospective multicenter international surveillance of azole resistance in Aspergillus fumigatus
To investigate azole resistance in clinical Aspergillus isolates, we conducted prospective multicenter international surveillance. A total of 3,788 Aspergillus isolates were screened in 22 centers from 19 countries. Azole-resistant A. fumigatus was more frequently found (3.2% prevalence) than previo …
Have allergic aspergillosis. Would a flare up result in low blood oxygen level?
Question - Have allergic aspergillosis. Would a flare up result in low blood oxygen level?. Ask a Doctor about diagnosis, treatment and medication for Allergic bronchopulmonary aspergillosis, Ask an OBGYN, Gynecologic Oncology
Open-label Vitamin D Trial for Patients With Cystic Fibrosis and Allergic Bronchopulmonary Aspergillosis - Full Text View -...
Many patients with cystic fibrosis (CF) cough up mucus or have throat cultures that grow a common fungus called Aspergillus. In patients with CF, aspergillus is not known to cause direct damage to the lungs, but some patients respond with an allergic reaction that causes them to wheeze, cough, or have difficulty breathing. This allergic reaction is called ABPA. Current treatment for ABPA includes high dose steroids and an anti-fungal medicine. Treatment with steroids may be problematic for some people due to its side effects on blood sugar levels and the bones. Steroids are medications that decrease inflammation, including prednisone, medrol, dexamethasone and others.. Ongoing research at UPMC on the study Mechanisms of Immune Tolerance in ABPA has studied people with CF and ABPA versus those patients with CF that just grow A. fumigatus (Af) in the sputum, but do not have ABPA. You may have participated in this study. This study has shown that people with CF with the fungus, Af, in their ...
Biochemische Untersuchungen von Prenyltransferasen aus verschiedenen Ascomyceten
Aspergillus fumigatus, an opportunistic pathogenic fungus, is the major reason for invasive aspergillosis and constitute also as an allergenic origin. Despite of the importance of the pathogenity of this fungus, only little is known about the background of its pathogenity. A. fumigatus produces a series of secondary metabolites which can be alone or together responsible for the pathogenity. For example, this fungus produces gliotoxin, an epipolythiodixopiperazine, which is in vitro characterised as a potent agent for cell death. For this reason it was speculated, that this mycotoxin might be involved in the pathogenesis of A. fumigatus. It is still necessary to know more about the genetic information and biosynthesis of further secondary metabolites, which might function as virulence factors. The biosynthetic gene cluster of gliotoxin has been identified in A. fumigatus. In the genome sequence of A. fumigatus Af293, an additional putative biosynthetic gene cluster containing three genes with ...
GlycoNet investigators establish glycoside hydrolases as potential biofilm disruptors - Canadian Glycomics Network
By Jennifer Doucet & Anushka Jayasuriya - June 23, 2017. Microbes, including bacteria and fungi, have many tools to evade a host cells defenses and antimicrobial therapies. One tool is the creation of protective barriers called biofilms, made of proteins, DNA, and sugar polymers, which spread across surfaces and impede immune system responses and antimicrobial treatments. In fact, biofilms can make microbes up to 1000 times more resistant to treatment. Despite their critical role in resistance to antimicrobials, there are no currently licensed therapies that target mature biofilms.. The bacteria Pseudomonas aeruginosa and the fungus Aspergillus fumigatus both form sugar-rich biofilms in host tissues during infection. They cause serious infections worldwide, especially in immunocompromised patients or those with chronic disease. Additionally, co-colonization by both P. aeruginosa and A. fumigatus has been observed in patients with lung disease such as cystic fibrosis.. As recently published in ...
Treating allergic bronchopulmonary aspergillosis with short-term prednisone and itraconazole in cystic fibrosis. | Physicians...
The used ABPA treatment regimen restored forced expiratory volume in 1 second (FEV) values to pre-ABPA levels within three months (p
Allergic Bronchopulmonary Aspergillosis (ABPA) - Pulmonary Disorders - Merck Manuals Professional Edition
Learn about the causes, symptoms, diagnosis & treatment of Asthma and Related Disorders from the Professional Version of the Merck Manuals.
Allergic Bronchopulmonary Aspergillosis
Cardiology news, research and treatment articles offering cardiology healthcare professionals cardiology information and resources to keep them informed.
Jundishapur Journal of Microbiology | Investigating the Presence of Aspergillus fumigatus and A. flavus Using Galactomannan...
Invasive aspergillosis is still a major fungal infection in immunocompromised patients. On the other hand, on time diagnosis of disease in the primary stages of infection is highly imperative for appropriate antifungal therapy and decreases the mortality rate in suspected groups (16). There are several approaches for detection and diagnosis of IA in clinical samples of patients. However, conventional methods, including direct examination and culture, are the gold standard to identify the causing agents, but there are some limitations, such as being time consuming, lack of proper sensitivity in early stages of the disease, as well as false negative results when the patients take the antifungal drugs (17).. Molecular diagnostic methods including real-time PCR as well as GM- EIA are more sensitive and specific and can approve an early IA diagnosis clinical outcome, when the other diagnostic methods are negative. Moreover, rapid results could be offered due to GM- EIA and real-time PCR assay ...
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In recent years acquired azole resistance in Aspergillus fumigatus has been increasingly reported and a dominant mechanism of resistance (TR34/L98H) was found in clinical and environmental isolates. The aim of the present study was to investigate the prevalence of azole resistance in environmental A. fumigatus isolates collected in northern Italy. A. fumigatus grew from 29 of 47 soil samples analysed. Azole-resistant isolates were detected in 13% (6/47) of the soil samples and in 21% (6/29) of the soil samples containing A. fumigatus. High minimal inhibitory concentrations (MIC) of itraconazole (≥16 mg/L) and posaconazole (≥0.5 mg/L) were displayed by nine isolates from six different soil samples, namely apple orchard (1 sample), rose pot compost (2 samples), and cucurbit yields (3 samples). Seven isolates had a MIC=2 mg/L of voriconazole. Seven of nine itraconazole and posaconazole resistant isolates harboured the same TR34/L98H mutation of cyp51A. These findings, together with the occurrence of
Genome-wide transcriptomic analysis of a superior biomass-degrading strain of A. fumigatus revealed active lignocellulose...
Various saprotrophic microorganisms, especially filamentous fungi, can efficiently degrade lignocellulose that is one of the most abundant natural materials on earth. It consists of complex carbohydrates and aromatic polymers found in the plant cell wall and thus in plant debris. Aspergillus fumigatus Z5 was isolated from compost heaps and showed highly efficient plant biomass-degradation capability. The 29-million base-pair genome of Z5 was sequenced and 9540 protein-coding genes were predicted and annotated. Genome analysis revealed an impressive array of genes encoding cellulases, hemicellulases and pectinases involved in lignocellulosic biomass degradation. Transcriptional responses of A. fumigatus Z5 induced by sucrose, oat spelt xylan, Avicel PH-101 and rice straw were compared. There were 444, 1711 and 1386 significantly differently expressed genes in xylan, cellulose and rice straw, respectively, when compared to sucrose as a control condition. Combined analysis of the genomic and transcriptomic
KIT - Other Aspergillus groups
Axel Brakhage. Director of the Leibniz-Institute for Natural Product Research and Infection Biology - Hans-Knoell-Institute (HKI). and. Professor and Chair of Microbiology / Molecular Biology. Friedrich-Schiller-University Jena. Department Molecular and Applied Microbiology. Research. Pathogenicity of Aspergillus fumigatus and secondary metabolism of Aspergillus nidulans. ...
Yeast Contamination Toddler - Yeast Infection Tips
Healthwise knowledgebase health facts. This records does no longer update the recommendation of a physician. Healthwise, incorporated, disclaims any guarantee or legal responsibility to your use of this data.. situations we treat nyu langone scientific middle. Nyu langone medical doctors and other professionals deal with heaps of situations, sicknesses, illnesses, and accidents. Learn extra.. Set definition of set by using the unfastened dictionary. Set (sĕt) n. Mythology variant of seth2. Set 1 (sĕt) v. Set, putting, sets v.Tr. 1. To put in a specified position or arrangement; region set a e-book on a table.. # diabetes causes yeast infection diabetic. ★ diabetes causes yeast infection ★ diabetic stuffed peppers the 3 step trick that reverses diabetes completely in as little as eleven days.[ diabetes reasons.. Aspergillus fumigatus and aspergillosis pubmed central. Aspergillus fumigatus is a saprophytic fungus that plays an critical position in recycling environmental carbon and nitrogen ...
Severe Candida glabrata pancolitis and fatal Aspergillus fumigatus pulmonary infection in the setting of bone marrow aplasia...
Prolonged myelosuppression following CD19-directed CAR T-cell transfusion represents an important, yet underreported, adverse event. The resulting neutropenia and multifactorial immunosuppression can facilitate severe infectious complications. We describe the clinical course of a 59-year-old patient with relapsed/refractory DLBCL who received Axicabtagene-Ciloleucel (Axi-cel). The patient developed ASTCT grade I CRS and grade IV ICANS, necessitating admission to the neurological ICU and prolonged application of high-dose corticosteroids and other immunosuppressive agents. Importantly, neutropenia was profound (ANC | 100/μl), G-CSF-refractory, and prolonged, lasting more than 50 days. The patient developed severe septic shock 3 weeks after CAR transfusion while receiving anti-fungal prophylaxis with micafungin. His clinical status stabilized with broad anti-infective treatment and intensive supportive measures. An autologous stem cell backup was employed on day 46 to support hematopoietic recovery.
Aspergillus fumigatus PCR - Gribbles Veterinary
Species: Bovine, equine, canine and cats. Specimen: Depends on site of infection, e.g. foetal stomach content in abortion cases, swabs from nasal infections, tissue biopsies.. Container: Sterile container with tissue, fluid, swab. Collection protocol: Depends on sample type may involve removal of cotyledons or tissue biopsy, aspiration of fluid, nasal swab.. Special handling/shipping requirements: Dry swab samples are stable at room temperature. Other samples are to be transported and stored chilled. Do not freeze samples.. General information about the disease: Can cause site-specific mycotic disease e.g. mycotic abortion in cattle, guttural pouch mycosis in horses, nasal infections in dogs and cats. Infection is acquired from environmental sources, generally inhalation or ingestion. It is an opportunistic pathogen depending on impaired, overwhelmed or by-passed host defences to permit hyphal invasion of the tissues. Comparison with other related tests: PCR provides rapid detection and specific ...
3CHC: Crystal Structure Of Aspergillus Fumigatus Chitinase B1 In Complex With Monopeptide - NCBI structure
Chitinase inhibitors have chemotherapeutic potential as fungicides, pesticides, and antiasthmatics. Argifin, a natural product cyclopentapeptide, competitively inhibits family 18 chitinases in the nanomolar to micromolar range and shows extensive substrate mimicry
The genome sequences of three Aspergillus species-Aspergillus fumigatus, Aspergillus nidulans, and Aspergillus oryzae-were ... Aspergillus fumigatus is a species of fungus in the genus Aspergillus, and is one of the most common Aspergillus species to ... Emergence of Azole Resistance in Aspergillus fumigatus and Spread of a Single Resistance Mechanism. at SciVee The Aspergillus ... fumigatus in the mammalian host. Aspergillus fumigatus must acquire nutrients from its external environment to survive and ...
Aspergillus fumigatus non-coding RNAs
Aspergillus fumigatus is a pathogenic filamentous fungus and is responsible for more infections worldwide than any other mould ... May 2008). "Small ncRNA transcriptome analysis from Aspergillus fumigatus suggests a novel mechanism for regulation of protein ... The table below summarised the type, genome location and corresponding target sites of the ncRNAs identified in A. fumigatus. ... Comparative genomics showed that the snoRNAs present within A. fumigatus are conserved across other strains of fungus. This ...
Some Aspergillus species cause serious disease in humans and animals. The most common pathogenic species are A. fumigatus and A ... Several species of Aspergillus, including A. niger and A. fumigatus, will readily colonise buildings, favouring warm and damp ... Genome sizes for sequenced species of Aspergillus range from about 29.3 Mb for A. fumigatus to 37.1 Mb for A. oryzae, while the ... Aspergillus can cause neonatal infections. A. fumigatus (the most common species) infections are primary pulmonary infections ...
lycopersici and Aspergillus fumigatus. Streptomyces rochei produces moenomycin and bambermycin. Streptomyces rochei produces ...
Marshall AC, Kidd SE, Lamont-Friedrich SJ, Arentz G, Hoffmann P, Coad BR, Bruning JB (March 2019). "Aspergillus fumigatus ...
Lamoth, Frederic; Steinbach, William J. (2016-03-18). Advances in Aspergillus fumigatus pathobiology. Frontiers Media SA. ISBN ... Aspergillus brevistipitatus is a species of fungus in the genus Aspergillus. It is from the Fumigati section. It was first ... "Aspergillus brevistipitatus". www.mycobank.org. "Aspergillus brevistipitatus". www.uniprot.org. ... "New species in Aspergillus section Fumigati from reclamation sites in Wyoming (U.S.A.) and revision of A. viridinutans complex ...
... www.aspergillus.org.uk. Lamoth, Frederic; Steinbach, William J. (2016). Advances in Aspergillus fumigatus pathobiology. ... "Aspergillus persii , Aspergillus & Aspergillosis Website". www.aspergillus.org.uk. "Aspergillus persii , Aspergillus & ... Aspergillus persii growing on CYA plate Aspergillus persii growing on MEAOX plate "Aspergillus persii". www.mycobank.org. " ... Aspergillus persii is a species of fungus in the genus Aspergillus which can cause onychomycosis. It is from the Circumdati ...
64(3):515-517 Lamoth, Frederic; Steinbach, William J. (2016). Advances in Aspergillus fumigatus pathobiology. Frontiers Media ... 78:141-173 "Aspergillus cejpii". www.uniprot.org. "A. cejpii , Aspergillus & Aspergillosis Website". www.aspergillus.org.uk. ... Aspergillus cejpii is a species of fungus in the genus Aspergillus. It is from the Clavati section. The species was first ... Aspergillus cejpii growing on CYA plate Aspergillus cejpii growing on MEAOX plate Samson R.A.; Visagie, C.M.; Houbraken, J.; ...
"Aspergillus spinosus Kozak". www.gbif.org. Lamoth, Frederic; Steinbach, William J. (2016). Advances in Aspergillus fumigatus ... Aspergillus spinosus is a species of fungus in the genus Aspergillus. Aspergillus spinosus produces aszonalenins, 2- ... Aspergillus spinosus growing on CYA plate Aspergillus spinosus growing on MEAOX plate "Aspergillus spinosus". MycoBank. ... Powell, Keith A.; Renwick, Annabel; Peberdy, John F. (2013). The Genus Aspergillus: From Taxonomy and Genetics to Industrial ...
Frederic, Lamoth; William J., Steinbach (2016). Advances in Aspergillus fumigatus pathobiology. Frontiers Media SA. ISBN 978-2- ... Aspergillus marvanovae growing on CYA plate Aspergillus marvanovae growing on MEAOX plate "Aspergillus marvanovae". Www. ... Aspergillus marvanovae is a species of fungus in the genus Aspergillus which has been isolated from water with high boracic ... "Aspergillus marvanovae". Www.uniprot.org. Hubka, V.; Peterson, S. W.; Frisvad, J. C.; Yaguchi, T.; Kubatova, A.; Kolarik, M. ( ...
Lamoth, Frederic; Steinbach, William J. (2016). Advances in Aspergillus fumigatus pathobiology. Frontiers Media SA. ISBN ... Aspergillus coreanus is a species of fungus in the genus Aspergillus. It is from the Fumigati section. Several fungi from this ... Aspergillus coreanus growing on CYA plate Aspergillus coreanus growing on MEAOX plate Hong, S.-B.; Cho, H.-S.; Shin, H.-D.; ... 56:477-486 Samson, R.A.; Hong, S.; Peterson, S.W.; Frisvad, J.C.; Varga, J. (2007). "Polyphasic taxonomy of Aspergillus section ...
Heddergott, C.; Calvo, A. M.; Latgé, J. P. (2017-02-02). "The Volatome of Aspergillus fumigatus". Eukaryotic Cell. 13 (8): 1014 ...
Frederic, Lamoth; William J., Steinbach (2016). Advances in Aspergillus fumigatus pathobiology. Frontiers Media SA. ISBN 978-2- ... Aspergillus brevipes growing on CYA plate Aspergillus brevipes growing on MEAOX plate "Aspergillus brevipes". www.mycobank.org ... Aspergillus brevipes is an anamorph species of fungus in the genus Aspergillus. It is from the Fumigati section. It was first ... Aspergillus brevipes n. sp. Transactions of the British Mycological Society. 35(4):241-242 "Aspergillus brevipes". www.uniprot. ...
"Aspergillus australensis". www.mycobank.org. Frederic, Lamoth; William J., Steinbach (2016). Advances in Aspergillus fumigatus ... Aspergillus australensis is a species of fungus in the genus Aspergillus. It is from the Fumigati section. The species was ... Aspergillus australensis growing on CYA plate Aspergillus australensis growing on MEAOX plate Samson, R.A.; Hong, S.; Peterson ... Aspergillus australensis produces aszonalenins and wortmannins. A. australensis has been cultivated on both Czapek yeast ...
Frisvad, J.C.; Larsen, T.O. (2016). "Extrolites of Aspergillus fumigatus and Other Pathogenic Species in Aspergillus Section ... Aspergillus waksmanii growing on CYA plate Aspergillus waksmanii growing on MEAOX plate "Aspergillus waksmanii". www.uniprot. ... Aspergillus waksmanii is a homothallic species of fungus in the genus Aspergillus which has been isolated from soil from New ... Frederic, Lamoth; William J., Steinbach (2016). Advances in Aspergillus fumigatus pathobiology. Frontiers Media SA. ISBN 978-2- ...
Latgé JP, Chamilos G (December 2019). "Aspergillus fumigatus and Aspergillosis in 2019". Clinical Microbiology Reviews. 33 (1 ... Aspergillus is a fungus, commonly associated with respiratory infection. Candida albicans is a species of fungus that is ...
"Bioactive alkaloids from endophytic Aspergillus fumigatus". J Nat Prod. 72 (4): 753-5. doi:10.1021/np800700e. PMID 19256529. ... Hawas UW, El-Beih AA, El-Halawany AM (2012). "Bioactive anthraquinones from endophytic fungus Aspergillus versicolor isolated ... Fungi portal Bacillus isolates Biotechnology in pharmaceutical manufacturing Mycorrhiza Aspergillus oryzae, Saccharomyces ... two benzylazaphilones with an unprecedented carbon skeleton from the gorgonian-derived fungus Aspergillus sp". Bioorg Med Chem ...
Ge HM; Yu ZG; Zhang J; Wu JH; Tan RX (2009). "Bioactive alkaloids from endophytic Aspergillus fumigatus". J Nat Prod. 72 (4): ...
Rigbers O, Li SM (October 2008). "Ergot alkaloid biosynthesis in Aspergillus fumigatus. Overproduction and biochemical ... "An old yellow enzyme gene controls the branch point between Aspergillus fumigatus and Claviceps purpurea ergot alkaloid ... "Ergot cluster-encoded catalase is required for synthesis of chanoclavine-I in Aspergillus fumigatus". Current Genetics. 57 (3 ... Chanocalvine-I is then oxidized to chanoclavine-I aldehyde with NAD+ dependent enzyme EasD (FgaDH in A. fumigatus). ...
Rigbers O, Li SM (October 2008). "Ergot alkaloid biosynthesis in Aspergillus fumigatus. Overproduction and biochemical ...
Aspergillus fumigatus, is a heterothallic fungus. It is one of the most common Aspergillus species to cause disease in humans ... Examples of heterothallism are included for Saccharomyces cerevisiae, Aspergillus fumigatus, Aspergillus flavus, Penicillium ... "Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus fumigatus". Nature. 457 (7228): 471-4. Bibcode: ... "Low genetic variation and no detectable population structure in aspergillus fumigatus compared to closely related Neosartorya ...
Ge HM, Yu ZG, Zhang J, Wu JH, Tan RX (2009). "Bioactive alkaloids from endophytic Aspergillus fumigatus". J Nat Prod. 72 (4): ... 14-Norpseurotin A is an alkaloid and a bio-active metabolite of Aspergillus, featuring an oxa-spiro-lactam core. Pseurotin A ... "Antiparasitic and anticancer constituents of the endophytic fungus Aspergillus sp. strain F1544". Nat Prod Commun. 7 (2): 165-8 ... Aspergillus, Spiro compounds, All stub articles, Organic compound stubs). ...
2(3):355-364 Lamoth, F. (2016). "Aspergillus fumigatus-Related Species in Clinical Practice". Frontiers in Microbiology. 7: 683 ... Aspergillus viridinutans is a species of fungus in the genus Aspergillus. The species was first isolated in Frankston, Victoria ... Aspergillus viridinutans growing on CYA plate Aspergillus viridinutans growing on MEAOX plate McLennan, E.I.; Ducker, S.C.; ... Samson, R.A.; Hong, S.; Peterson, S.W.; Frisvad, J.C.; Varga, J. (2007). "Polyphasic taxonomy of Aspergillus section Fumigati ...
In Aspergillus fumigatus, a causative agent of aspergillosis, galactosaminogalactan is required for adherence to host tissue, ... Overlapping and distinct roles of Aspergillus fumigatus UDP-glucose 4-epimerases in galactose metabolism and the synthesis of ... 2014). A polysaccharide virulence factor from Aspergillus fumigatus elicits anti-inflammatory effects through induction of ... 2011). Galactosaminogalactan, a new immunosuppressive polysaccharide of Aspergillus fumigatus. In PLoS Pathog, pp. e1002372 ...
Friedrich Siebenmann (otolaryngologist)
Die fadenpilze, aspergillus flavus, niger u. fumigatus; eurotium repens (u. aspergillus glaucus) und ihre beziehungen zur ... otomycosis aspergillina, 1883 - The filamentous fungi, Aspergillus flavus, niger and fumigatus; Eurotium repens (Aspergillus ...
The fungus Aspergillus fumigatus degrades plasticized PVC. Phanerochaete chrysosporium was grown on PVC in a mineral salt agar ... Phanerochaete chrysosporium, Lentinus tigrinus, Aspergillus niger, and Aspergillus sydowii can effectively degrade PVC. ...
In Aspergillus fumigatus, the enzymes needed for gliotoxin biosynthesis are encoded in 13 genes within the gli gene cluster. ... The compound is produced by human pathogens such as Aspergillus fumigatus, and also by species of Trichoderma and Penicillium. ... Gliotoxin is hypothesized to be an important virulence factor in Aspergillus fumigatus. Experiments have demonstrated that ... gliotoxin is isolated in the highest concentrations from Aspergillus fumigatus in comparison to other Aspergillus species. This ...
Cavernous sinus thrombosis
Rarely, Aspergillus fumigatus and mucormycosis cause CST. Aseptic cavernous sinus thrombosis is much less ...
... is a species of Aspergillus fungus. It is a close relative of Aspergillus fumigatus. It has smaller ... Aspergillus lentulus growing on CYA plate Aspergillus lentulus growing on MEAOX plate Balajee, S. Arunmozhi; Jennifer L. ... Aspergillus lentulus is an opportunistic human pathogen that causes invasive aspergillosis with high mortality rates. It has ... Swilaiman SS, O'Gorman CM, Balajee SA, Dyer PS (July 2013). "Discovery of a sexual cycle in Aspergillus lentulus, a close ...
Polyphasic taxonomy of Aspergillus fumigatus and related species. Mycologia. 97(6):1316-1329 Samson, R.A.; Hong, S.; Peterson, ... Aspergillus fumigatiaffinis is a species of fungus in the genus Aspergillus. It is from the Fumigati section. The species was ... v t e (Articles with short description, Short description matches Wikidata, Articles with 'species' microformats, Aspergillus, ... S.W.; Frisvad, J.C.; Varga, J. (2007). "Polyphasic taxonomy of Aspergillus section Fumigati and its teleomorph Neosartorya". ...
2017). "Pharmacodynamics of the Orotomides against Aspergillus fumigatus: New Opportunities for Treatment of Multidrug- ... while searching for a new drug for Aspergillus infection. The discovery was formally announced at the Interscience Conference ... It was shown to be useful for acute sinopulmonary aspergillosis caused by Aspergillus flavus. Birch, Michael (19 September 2015 ... It was found to be effective against most important human fungal infections including those with Aspergillus, Lemontospora ( ...
List of Aspergillus species
Aspergillus fulvus Aspergillus fumaricus Aspergillus fumigatiaffinis Aspergillus fumigatoides Aspergillus fumigatus Aspergillus ... U V W X Y Z Aspergillus racemosus Aspergillus raianus Aspergillus rambellii Aspergillus ramosus Aspergillus raperi Aspergillus ... recurvatus Aspergillus rehmii Aspergillus repandus Aspergillus repens Aspergillus reptans Aspergillus restrictus Aspergillus ... U V W X Y Z Aspergillus udagawae Aspergillus umbrinus Aspergillus umbrosus Aspergillus undulatus Aspergillus unguis Aspergillus ...
List of MeSH codes (B05)
... aspergillus fumigatus MeSH B05.381.081.420 - aspergillus nidulans MeSH B05.381.081.450 - aspergillus niger MeSH B05.381.081.480 ... aspergillus ochraceus MeSH B05.381.081.500 - aspergillus oryzae MeSH B05.381.125 - blastomyces MeSH B05.381.128 - botrytis MeSH ... aspergillus MeSH B05.381.081.170 - aspergillus flavus MeSH B05.381.081.295 - ...
Coumarin Dicumarol Bye, A.; King, H. K. (1970). "The biosynthesis of 4-hydroxycoumarin and dicoumarol by Aspergillus fumigatus ...
Effects of cannabis
Aspergillus fumigatus, Aspergillus niger, Aspergillus parasiticus, Aspergillus tamarii, Aspergillus sulphureus, Aspergillus ... Aspergillus mold species can infect the lungs via smoking or handling of infected cannabis and cause opportunistic and ... Oven treatment killed conidia of A. fumigatus, A. flavus and A. niger, and did not degrade the active component of marijuana, ...
Aspergillus fumigatus and Aspergillus clavatus can cause allergic disease. Some Aspergillus species cause disease on grain ... The most common pathogenic species are Aspergillus fumigatus and Aspergillus flavus. Aspergillus flavus produces aflatoxin ... The spores of Aspergillus fumigatus are ubiquitous in the atmosphere. A. fumigatus is an opportunistic pathogen. It can cause ... O'Gorman CM, Fuller H, Dyer PS (2009). "Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus fumigatus ...
... voriconazole led to acute kidney injury upon admission of a 41-year-old man who was dually infected by Aspergillus fumigatus ... This rare case provides some insight into potential treatment protocols for M. microspora and A. fumigatus infections in humans ...
Despite so, certain organisms such as fungi Aspergillus fumigatus is capable of incorporating tellurocysteine and ...
Molds such as Trichoderma, Exophiala, Stachybotrys, Aspergillus fumigatus, Aspergillus versicolor, Phialophora, Fusarium, ... Penicillium species, Aspergillus niger and Candida albicans are used in the pharmaceutical industry for microbial limit testing ...
Non-proteinogenic amino acids
Amino acids with the next chalcogen down are also found in nature: several species such as Aspergillus fumigatus, Aspergillus ...
... and Inhibition of Aspergillus fumigatus Thioredoxin Reductase". Antimicrobial Agents and Chemotherapy. 63 (3): e02281-18. doi: ... against Clostridioides difficile infections and has been shown to have antifungal activity against Aspergillus fumigatus. ...
... active against Aspergillus fumigatus as well as Gram-positive and Gram-negative bacteria. Other metabolites have shown activity ...
Dunlap continues to investigate the circadian clock, using Neurospora and other organisms, such as Aspergillus fumigatus. As a ... "Aspergillus fumigatus Photobiology Illuminates the Marked Heterogeneity between Isolates". mBio. 7 (5). doi:10.1128/mBio.01517- ...
"Genetic Relatedness versus Biological Compatibility between Aspergillus fumigatus and Related Species". Journal of Clinical ... Aspergillus pseudoviridinutans is a species of fungus in the genus Aspergillus. It is from the Fumigati section. Several fungi ... Aspergillus pseudoviridinutans growing on CYA plate Aspergillus pseudoviridinutans growing on MEAOX plate Sugui, J.A.; Peterson ... Samson, R.A.; Hong, S.; Peterson, S.W.; Frisvad, J.C.; Varga, J. (2007). "Polyphasic taxonomy of Aspergillus section Fumigati ...
Calcium-dependent chloride channel
A single protein homologue to the vertebrate anoctamins has been found in fungi and yeast, Aspergillus fumigatus and ...
... and low to moderate activity against the fungi Candida albicans and Aspergillus fumigatus. The fungus produces the natural ...
Frisvad, J.C.; Larsen, T.O. (2016). "Extrolites of Aspergillus fumigatus and Other Pathogenic Species in Aspergillus Section ... Aspergillus solicola is a species of fungus in the genus Aspergillus. It is from the Fumigati section. Several fungi from this ... Samson, R.A.; Hong, S.; Peterson, S.W.; Frisvad, J.C.; Varga, J. (2007). "Polyphasic taxonomy of Aspergillus section Fumigati ... identification and nomenclature of the genus Aspergillus". Studies in Mycology. 78: 141-173. doi:10.1016/j.simyco.2014.07.004. ...
... a potential substrates for laccase production by Aspergillus fumigatus VkJ2.4.5 in solid-state fermentation. Applied ...
Kremer A, Westrich L, Li SM (October 2007). "A 7-dimethylallyltryptophan synthase from Aspergillus fumigatus: overproduction, ...
Frisvad, J.C.; Larsen, T.O. (2016). "Extrolites of Aspergillus fumigatus and Other Pathogenic Species in Aspergillus Section ... Aspergillus udagawae is a species of fungus in the genus Aspergillus. It is from the Fumigati section. Several fungi from this ... Aspergillus udagawae growing on CYA plate Aspergillus udagawae growing on MEAOX plate Horie, Y.; Miyaki, M.; Nishimura, K.; ... Samson, R.A.; Hong, S.; Peterson, S.W.; Frisvad, J.C.; Varga, J. (2007). "Polyphasic taxonomy of Aspergillus section Fumigati ...
... is an inflammatory disease caused by hypersensitivity to the fungus Aspergillus fumigatus. It is suspected in patients with a ... De Soyza, Anthony; Aliberti, Stefano (2017-01-01). "Bronchiectasis and Aspergillus: How are they linked?". Medical Mycology. 55 ...
Initially, rapamycin was developed as an antifungal drug against Candida albicans, Aspergillus fumigatus and Cryptococcus ...
L-ornithine N5 monooxygenase
Chocklett SW, Sobrado P (August 2010). "Aspergillus fumigatus SidA is a highly specific ornithine hydroxylase with bound flavin ... Crystallographic structures have been solved for this class of enzymes from Aspergillus fumigatus. These structures reflect ... it is also found in many fungal pathogens such as Aspergillus fumigatus, Botrytis cinerea, Fusarium oxysporum, Magnaporthe ... In A. fumigatus, it is classified as a flavoprotein because FAD is a cofactor. It catalyzes the FAD and NADPH-dependent ...
Screening for Triazole-Resistant Aspergillus fumigatus Using Agar Plates | Fungal Diseases | CDC
... fumigatus for azole resistance is to use an agar plate assay that includes antifungal drugs in the agar. Growth on the agar ... One way to screen isolates of Aspergillus fumigatus for azole resistance is to use an agar plate assay that includes antifungal ... This procedure will be used for screening Aspergillus fumigatus isolates for azole antifungal resistance using a 4-quadrant ... The environmental mold Aspergillus fumigatus is the primary cause of invasive aspergillosis. In patients with high-risk ...
Aspergillus fumigatus: saprophyte or pathogen? - Research - Institut Pasteur
Large-scale genome comparisons have shown that no gene sets are shared exclusively by both Aspergillus fumigatus and any other ... fumigatus genome. In addition, the proteome of this fungus contains numerous efflux pumps, including ,100 major facilitators ... fumigatus in an unfriendly environment such as its human host. ...
4CAV: Crystal Structure Of Aspergillus Fumigatus N-myristoyl Transferase In Complex With Myristoyl Coa And A Benzofuran Ligand...
Environmental Hotspots for Azole Resistance Selection of Aspergillus fumigatus, the Netherlands - Volume 25, Number 7-July 2019...
Azole-resistant Aspergillus fumigatus in sawmills of Eastern France. J Appl Microbiol. 2017;123:172-84. DOIPubMedGoogle Scholar ... Aspergillus fumigatus and aspergillosis. Clin Microbiol Rev. 1999;12:310-50. DOIPubMedGoogle Scholar ... Azole resistance in Aspergillus fumigatus: a side-effect of environmental fungicide use? Lancet Infect Dis. 2009;9:789-95. DOI ... Azole-resistant Aspergillus fumigatus harboring TR34/L98H, TR46/Y121F/T289A and TR53 mutations related to flower fields in ...
Substrate Specifity Profiling of the Aspergillus Fumigatus Proteolytic Secretome Reveals Consensus Motifs with Predominance of...
In vitro Protease Inhibition and Cytotoxicity of Aspergillus fumigatus Biomolecules Secreted under Long-Term Aerated Conditions...
Keywords: Aspergillus fumigatus, culture filtrate, oxygen, cytotoxicity, cysteine protease inhibition Citation styles. APA Copy ... Aspergillus fumigatus is the main causative agent of IA and investigation of its metabolites could provide valuable insight ... In vitro Protease Inhibition and Cytotoxicity of Aspergillus fumigatus Biomolecules Secreted under Long-Term Aerated Conditions ... In vitro Protease Inhibition and Cytotoxicity of Aspergillus fumigatus Biomolecules Secreted under Long-Term Aerated Conditions ...
m218 Aspergillus fumigatus (alergen recombinat: rAsp f1) - Synevo
Analiza medicala m218 Aspergillus fumigatus (alergen recombinat: rAsp f1) este utilizata in diagnosticul alergiilor, ... m218 Aspergillus fumigatus (alergen recombinat: rAsp f1), IgE specific. Pret 77.00 lei. ... Home / Shop / Teste specializate de alergologie si imunologie / IgE specifice / m218 Aspergillus fumigatus (alergen recombinat ...
Determining whether estimated spore release rates for Aspergillus fumigatus are compatible with their measured growth rates in...
... experiments using compost samples and Aspergillus fumigatus spores to determine the sporulation rate of Aspergillus fumigatus. ... A series of experiments were carried out using Aspergillus fumigatus spores on agar plates and small samples of green waste ... There has been considerable interest in the literature of the effect of bioaerosols and in particular Aspergillus fumigatus on ... Through the use of a portable wind tunnel apparatus researchers have determined the rate of emission of Aspergillus fumigatus ...
Calcineurin target CrzA regulates conidial germination, hyphal growth, and pathogenesis of Aspergillus fumigatus. | Scholars...
Calcineurin target CrzA regulates conidial germination, hyphal growth, and pathogenesis of Aspergillus fumigatus. Journal ... in the physiology and pathogenicity of the opportunistic human fungal pathogen Aspergillus fumigatus. Genetic replacement of ... Our results suggest that CrzA is an important downstream effector of calcineurin that controls morphology in A. fumigatus, but ... fumigatus DeltacnaA (calcineurin A) strain. Like the DeltacnaA strain, the DeltacrzA strain was incapable of causing disease in ...
Azole resistance surveillance in Aspergillus fumigatus: beneficial or biased?
Azole resistance is a growing concern with Aspergillus fumigatus, and may cause increased mortality in patients with azole- ... We believe that local resistance rates based on Aspergillus disease and/or risk group should be leading for decisions regarding ... Azole resistance frequencies have been based on screening of unselected A. fumigatus isolates, on the number of azole-resistant ... cases within a cohort of patients with a specific Aspergillus disease, or on analysis of patients within a specific risk group ...
Members of protein O-mannosyltransferase family in Aspergillus fumigatus differentially affect growth, morphogenesis and...
Aspergillus fumigatus. Genome analysis showed that only one member of each subfamily is present in A. fumigatus, PMT1, PMT2 and ... These results indicate that the PMT family members play a major role in growth, morphogenesis and viability of A. fumigatus. ... fumigatus. If disruption of PMT1 did not reveal any phenotype, deletion of PMT2 was lethal. Disruption of PMT4 resulted in ... Aspergillus fumigatus. Genome analysis showed that only one member of each subfamily is present in A. fumigatus, PMT1, PMT2 and ...
Pancreatic abscess due to <i>Aspergillus fumigatus</i> :[PAUTHORS], Journal of...
Pancreatic abscess due to Aspergillus fumigatus. G Barreto1, J Rodrigues2, RG W Pinto3, S Rodrigues4, MJ Rodrigues5, V Mallaya6 ... The pus, which was sent for staining and culture, revealed the causative organism to be Aspergillus fumigatus How to cite this ... Pancreatic abscess due to Aspergillus fumigatus. J Cytol [serial online] 2005 [cited 2022 Dec 8 ];22:191-193 Available from: ... Pancreatic abscess due to Aspergillus fumigatus.J Cytol 2005;22:191-193 ...
Aspergillus fumigatus gca 002234985 - Ensembl Genomes 55
About Aspergillus fumigatus (GCA_002234985) Aspergillus fumigatus is a species of fungus in the genus Aspergillus, and is one ... Aspergillus fumigatus, a saprotroph widespread in nature, is typically found in soil and decaying organic matter, such as ... Aspergillus fumigatus grown on certain building materials can produce genotoxic and cytotoxic mycotoxins, such as gliotoxin. ... Although A. fumigatus occurs in areas with widely different climates and environments, it displays low genetic variation and a ...
The involvement of the Mid1/Cch1/Yvc1 calcium channels in Aspergillus fumigatus virulence - Fingerprint - the University...
Molecular typing by random amplification of polymorphic DNA and M13 southern hybridization of related paired isolates of...
RAPD with four primers (R108, RC08, 2, and 4), including three previously used with A. fumigatus, showed that one primer, R108 ... A full understanding of the epidemiology and ecology of A. fumigatus requires multiple discriminatory typing procedures. ... Three forms of DNA-based typing procedures for Aspergillus fumigatus isolates have been developed over the last five years. The ... Three forms of DNA-based typing procedures for Aspergillus fumigatus isolates have been developed over the last five years. The ...
Azole Resistance of Aspergillus fumigatus in Immunocompromised Patients with Invasive Aspergillosis
... Cite ... Title : Multiple Fungicide-Driven Alterations in Azole-Resistant Aspergillus fumigatus, Colombia, 2015 Personal Author(s) : Le ... Multiple Fungicide-Driven Alterations in Azole-Resistant Aspergillus fumigatus, Colombia, 2015 Cite ... Title : Azole Resistance of Aspergillus fumigatus in Immunocompromised Patients with Invasive Aspergillosis Personal Author(s ...
Studies on Aspergillus fumigatus antigens for serodiagnosis - Publications of the IAS Fellows
Banerjee, B. ; Mukherjee, M. ; Gangal, S. V. ; Sarma, P. U. (1990) Studies on Aspergillus fumigatus antigens for serodiagnosis ... Aspergillus fumigatus Antigens; Allergic Bronchopulmonary Aspergillosis; Fast Protein Liquid Chromatography; ELISA. ID Code:. ... Culture filtrate (CF) proteins of a soil strain of Aspergillus fumigatus were evaluated for their potential use in the ... The patients with strong precipitin reaction showed high serum IgG levels against A. fumigatus antigens. ...
Regulation der Eisenhomöostase in Aspergillus fumigatus - Leibniz Institute for Natural Product Research and Infection Biology ...
Aspergillus flavus: An emerging non-fumigatus Aspergillus species of significance<...
Aspergillus flavus: An emerging non-fumigatus Aspergillus species of significance. Mycoses. 2009 May 1;52(3):206-222. doi: ... Aspergillus flavus: An emerging non-fumigatus Aspergillus species of significance. Suganthini Krishnan, Elias Kurian Manavathu ... Aspergillus flavus : An emerging non-fumigatus Aspergillus species of significance. / Krishnan, Suganthini; Manavathu, Elias ... title = "Aspergillus flavus: An emerging non-fumigatus Aspergillus species of significance",. abstract = "Invasive ...
aspergillus fumigatus - Just Say Mo Foundation
Posts about aspergillus fumigatus written by Mo Gesualdi ... Tagged April, aspergillus fumigatus, change lives, cope, donate ... Tagged aspergillus, aspergillus fumigatus, brain, brain infection, Channel 3, connecticut, ct, danbury, Danbury CT, donate life ... Tagged Annie Lake, aspergillosis, aspergillus fumigatus, donate life, golf, invasive aspergillus, liver transplant, LPGA, mo ... This monster of a fungal-infection had a name, and it was called aspergillus fumigatus; all I knew is that the aspergillus had ...
Home - Aspergillus fumigatus Af293 from AspGD
Please confirm that you want to SAVE all your changes for Aspergillus fumigatus Af293 from AspGD. ... Please confirm that you want to DISCARD all your changes for Aspergillus fumigatus Af293 from AspGD. ... Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature. 2005 Dec 22;438(7071):1151- ... This copy of the genome of Aspergillus fumigatus was obtained from AspGD. ...
Vaccines | Free Full-Text | Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental...
... confers protection against infection by Aspergillus fumigatus, a major fungal pathogen that expresses α-Gal in its ... fumigatus. Oral administration of E. coli O86:B7 was not associated with the upregulation of lung cytokines upon A. fumigatus ... Aspergillus fumigatus in Poultry. Int. J. Microbiol. 2011, 746356. [Google Scholar]. *Latgé, J.P.; Chamilos, G. Aspergillus ... Aspergillus fumigatus Strain and Inoculum Preparation. The highly germinative A. fumigatus CBS 144.89 (CEA10) clinical strain ...
IMSEAR at SEARO: Perinephric abscess due to Aspergillus fumigatus.
UP Health System - Marquette Laboratory | ASPERGILLUS FUMIGATUS IGG AB
Virtual infection model of Aspergillus fumigatus in a murine alveolus
Aspergillus fumigatus is a ubiquitous opportunistic fungal pathogen that can cause severe infections in immunocompromised ... Adaptation of the previously developed virtual infection model of Aspergillus fumigatus in a *human* alveolus. This model ... Virtual infection model of Aspergillus fumigatus in a murine alveolus Version 1 ... Projects: B4, FungiNet A - Aspergillus projects, FungiNet B - Bioinformatics projects, FungiNet C - Candida projects, FungiNet ...
Multicenter Study of Azole-Resistant Aspergillus fumigatus Clinical Isolates, Taiwan - Volume 26, Number 4-April 2020 -...
Chen Y, Li Z, Han X, Tian S, Zhao J, Chen F, et al. Elevated MIC values of imidazole drugs against Aspergillus fumigatus ... Chowdhary A, Sharma C, van den Boom M, Yntema JB, Hagen F, Verweij PE, et al. Multi-azole-resistant Aspergillus fumigatus in ... Non-cyp51A azole-resistant Aspergillus fumigatus isolates with mutation in HMG-CoA reductase. Emerg Infect Dis. 2018;24:1889-97 ... Multicenter Study of Azole-Resistant Aspergillus fumigatus Clinical Isolates, Taiwan1 On This Page ...
Valorization of wheat bran for cost-effective production of cellulolytic enzymes by Aspergillus fumigatus SKH2 and utilization...
Aspergillus fumigatus during stable state and exacerbations of COPD. - Experimental Medicine Division
We investigated the frequency of filamentous fungal culture and IgE sensitisation to Aspergillus fumigatus and the relationship ... fumigatus. Fungus was cultured in three out of 22 controls (two were A. fumigatus). The total sputum cell count and inhaled ... A. fumigatus sensitisation is related to poor lung function. Positive filamentous fungal culture is a common feature of COPD. ... fumigatus was present in 13% of COPD subjects and was associated with worse lung function (forced expiratory volume in 1 s 39% ...
AspergillosisIsolatesFungiFungusInfectionGrowth of Aspergillus fumigatusCandidaPathogenResistancePulmonarySporesOpportunisticFlavusItraconazoleOrganismInfectionsAbscess dueGenomeConidiaMoldsTriazole-resistantFungal DiseasesSerumPatientsAntigensLungSusceptibilityDifferentiallyClinicalCystic fibrosisProteinsPenicilliumEmergencePrevalenceGeneticGenesNidulansConidialVitroMolecularMycetomaCompostMiceMycology
- The environmental mold Aspergillus fumigatus is the primary cause of invasive aspergillosis. (cdc.gov)
- Humans might inhale hundreds of A. fumigatus spores daily, but aspergillosis generally does not develop in healthy persons because the spores are eliminated by the innate immune response ( 1 ). (cdc.gov)
- Azole resistance is a growing concern with Aspergillus fumigatus, and may cause increased mortality in patients with azole-resistant invasive aspergillosis (IA). (ru.nl)
- Culture filtrate (CF) proteins of a soil strain of Aspergillus fumigatus were evaluated for their potential use in the serodiagnosis of patients of allergic bronchopulmonary aspergillosis (ABPA). (ias.ac.in)
- Among the most common causes of invasive aspergillosis and acute bronchopulmonary aspergillosis is Aspergillus fumigatus . (springeropen.com)
- Transmission with A. fumigatus produces aggressive aspergillosis in allogeneic haematopoietic stem cell transplant recipients, HIV patients, and cancer patients. (springeropen.com)
- The invasive aspergillosis and acute bronchopulmonary aspergillosis are caused by harmful fungal pathogen Aspergillus fumigatus . (springeropen.com)
- Aspergillosis is an infection caused by the fungus aspergillus. (medlineplus.gov)
- Aspergillus fumigatus is the most common cause of aspergillosis, followed by A. flavus and A. niger . (latesting.com)
- Severe bilateral chronic pulmonary aspergillosis with the left upper lobe replaced by one large and several smaller cavities and a fluid level (which on aspiration grew a pure growth of Aspergillus fumigatus). (life-worldwide.org)
- Aspergillus fumigatus , for example, is a common cause of a respiratory problem called invasive aspergillosis. (moldbacteriaconsulting.com)
- Minimum spanning tree comparing genotypic relatedness of clinical azole- resistant Aspergillus fumigatus isolates carrying TR34/L98H alteration in the CYP 51A gene from Iran with those reported from European countries. (cdc.gov)
- Triazole resistance among clinical Aspergillus fumigatus isolates. (cdc.gov)
- Azole resistance in Aspergillus fumigatus isolates from the ARTEMIS global surveillance study is primarily due to the TR/L98H mutation in the cyp51A gene. (cdc.gov)
- One way to screen isolates of Aspergillus fumigatus for azole resistance is to use an agar plate assay that includes antifungal drugs in the agar. (cdc.gov)
- The Centers for Disease Control and Prevention is interested in receiving US azole-resistant A. fumigatus isolates for surveillance. (cdc.gov)
- This procedure will be used for screening Aspergillus fumigatus isolates for azole antifungal resistance using a 4-quadrant agar plate with antifungals. (cdc.gov)
- This procedure outlines how to: 1) create 4-well screening plates, 2) process isolates, 3) inoculate Aspergillus screening plates and record the results, and 4) store the isolates. (cdc.gov)
- We previously demonstrated that 5 azole fungicides from the triazole class were highly active against wild-type A. fumigatus but showed no activity against resistant isolates with TR 34 /L98H ( 12 ). (cdc.gov)
- Azole resistance frequencies have been based on screening of unselected A. fumigatus isolates, on the number of azole-resistant cases within a cohort of patients with a specific Aspergillus disease, or on analysis of patients within a specific risk group. (ru.nl)
- Molecular typing by random amplification of polymorphic DNA and M13 southern hybridization of related paired isolates of Aspergillus fumigatus. (ox.ac.uk)
- Three forms of DNA-based typing procedures for Aspergillus fumigatus isolates have been developed over the last five years. (ox.ac.uk)
- To delineate the influence of clinical and environmental use of azoles on resistance, we conducted a multicenter study that investigated 375 A. fumigatus sensu stricto isolates collected during August 2011-March 2018 from 297 patients at 11 hospitals in Taiwan ( Appendix Table 1, Figure 1). (cdc.gov)
- Overall, 19 isolates from 12 patients were azole-resistant A. fumigatus . (cdc.gov)
- Ten (83.3%) patients harbored azole-resistant A. fumigatus that had environmental mutations, including TR 34 /L98H (5 isolates, 5 patients), TR 34 /L98H/S297T/F495I (7 isolates, 4 patients), and TR 46 /Y121F/T289A (1 isolate) mutations. (cdc.gov)
- Genetic relatedness among Aspergillus fumigatus isolates based on microsatellite genotyping, Taiwan. (cdc.gov)
- A recent article reported a 3.2% prevalence of Aspergillus fumigatus isolates that are resistant to azole from 3,788 isolates screened in Europe ( 2 ). (cdc.gov)
- Cultures of respiratory samples (i.e., bronchoalveolar lavage fluid, tracheal aspirate, and sputum) or biopsies were positive for 99 episodes: 68 with A. fumigatus isolates and 31 with other Aspergillus spp. (cdc.gov)
- Among the 68 A. fumigatus isolates, 1 (1.5%) associated with probable IA was resistant to azoles ( 5 ). (cdc.gov)
- When focusing on patients with probable or proven IA, we did not observe an emergence of azole-resistant A. fumigatus isolates during 2006-2009 ( 3 ) and 2012-2014 in France. (cdc.gov)
- Filamentous fungi were cultured at baseline in 49% (63 out of 128) of COPD subjects, of which 75% (47 out of 63) were A. fumigatus. (ox.ac.uk)
- It will be of high interest to study whether these regulatory interactions are also important for close relatives of A. fumigatus and other pathogenic fungi, such as Candida albicans . (biomedcentral.com)
- In current study, we targeted variety of proteins from A. fumigatus to screen out immunogenic T-cell epitopes against A. fumigatus fungi. (springeropen.com)
- Proteins of A. fumigatus fungi under consideration were enlisted in Table 1 . (springeropen.com)
- When analyzing the polyketide gene clusters from the sequenced dermatophyte genomes 1 , 8 , we found an orthologous gene cluster, which is also conserved in pathogenic fungi Aspergillus fumigatus and Neosartorya fischeri 10 , 11 ( Figure 1 ). (cdc.gov)
- 90% identity between them) ( Table S3, Supplementary Information ), and the lower sequence homology to those in A. fumigatus and N. fischeri (~70% identity, Table S3 ), raise an interesting question as to whether these fungi produce similar compounds, and whether the presence of additional conserved genes may lead to further tailoring of the neosartoricin scaffold. (cdc.gov)
- Many fungi such as Aspergillus niger, Aspergillus fumigatus, Candida tropicali are pathogenic fungi. (projectmaterial.net)
- By contrast, and in agreement with the environmental occurrence of this fungus in decaying vegetation, the enzymatic machinery required by a fungus to colonize plant substrates has been found in the A. fumigatus genome. (pasteur.fr)
- In this study, we characterized for the first time the whole PMT family of a pathogenic filamentous fungus, Aspergillus fumigatus. (archives-ouvertes.fr)
- Aspergillus fumigatus is a species of fungus in the genus Aspergillus , and is one of the most common Aspergillus species to cause disease in individuals with an immunodeficiency. (ensembl.org)
- Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. (doe.gov)
- Fungus was cultured in three out of 22 controls (two were A. fumigatus). (ox.ac.uk)
- BACKGROUND: Fungus ball (FB) typically affects healthy adults, and Aspergillus fumigatus is the most frequent etiologic agent: iatrogenic factors represent an important issue in FB pathogenesis. (units.it)
- These results suggested that a tightly regulated system for the fungus to grow under zinc-limiting conditions must underlie the ability of A. fumigatus to acquire zinc in such environments. (mediu.edu.my)
- Aspergillus fumigatus is a thermotolerant/thermophilic fungus capable of growing over a wide temperature range (12°C-53°C). It has a worldwide distribution. (moldbacteria.com)
- The most common species of fungus that causes disease in humans is Aspergillus fumigatus . (medlineplus.gov)
- Aspergillus is a common fungus. (medlineplus.gov)
- People who have had related lung infections or who have weakened immune systems should try to avoid environments where the aspergillus fungus is found. (medlineplus.gov)
- One of these also showed strong potential against Aspergillus fumigatus, a fungus affecting humans, especially immunocompromised patients. (dicyt.com)
- The specimen was supposed to be a species called Aspergillus fumigatus, a fungus that can cause life-threatening infections in transplant patients. (fredhutch.org)
- The noninvasive form called Aspergillus mycetoma (aspergilloma) or fungus ball occurs mostly in healthy individuals. (bvsalud.org)
- 3. Mellado E, De La Camara R, Buendia B, Rodriguez-Tudela JL, Cuenca-Estrella M. Breakthrough pulmonary Aspergillus fumigatus infection with multiple triazole resistance in a Spanish patient with chronic myeloid leukemia. (cdc.gov)
- Shortly after getting a transplant by the skin of my teeth, I barely escaped with my life again as I battled a rare and extremely fatal fungal infection in my brain called "Aspergillus Fumigatus. (justsaymo.net)
- Adaptation of the previously developed virtual infection model of Aspergillus fumigatus in a *human* alveolus. (hki-jena.de)
- Furthermore, in this model, depending on the infection dose multiple A. fumigatus conidium are inserted into the alveolus and the AEC, where the conidium is located secretes chemokines. (hki-jena.de)
- In the present study, we compare A. fumigatus infection in mice and humans by virtual infection modeling using a hybrid agent-based model that accounts for the respective lung physiology and the impact of a wide range of infection doses on the spatial infection dynamics. (hki-jena.de)
- Our computer simulations enable comparative quantification of A. fumigatus infection clearance in the two hosts to elucidate (i) the complex interplay between alveolar morphometry and the fungal burden and (ii) the dynamics of infection clearance, which for realistic fungal burdens is found to be more efficiently realized in mice compared to humans. (hki-jena.de)
- Our study highlights the diagnostic challenge and the possible impact of Aspergillus infection on these patients , indicating the necessity of more and larger investigations in the field. (bvsalud.org)
- Results: Netrin-1 expression decreased slightly after A. fumigatus infection and then increased to its peak. (mssm.edu)
- Correlation between in-vitro susceptibility testing to itraconazole and in-vivo outcome of Aspergillus fumigatus infection. (semanticscholar.org)
Growth of Aspergillus fumigatus1
- Endodontic materials containing zinc oxide are considered to cause growth of Aspergillus fumigatus and may allow for its proliferation and metabolism 2,4-9 . (bvsalud.org)
- Large-scale genome comparisons have shown that no gene sets are shared exclusively by both Aspergillus fumigatus and any other human pathogen sequenced to date, such as Candida or Cryptococcus species. (pasteur.fr)
- Indicated for prophylaxis of invasive Aspergillus and Candida infections in patients at high risk because of severe immunosuppression. (medscape.com)
- Aspergillus fumigatus: saprophyte or pathogen? (pasteur.fr)
- In this study, we examined the role of the zinc finger transcription factor Crz1 homolog (CrzA) in the physiology and pathogenicity of the opportunistic human fungal pathogen Aspergillus fumigatus. (duke.edu)
- Our results suggest that CrzA is an important downstream effector of calcineurin that controls morphology in A. fumigatus, but additional downstream effectors that mediate calcineurin signal transduction are likely present in this opportunistic fungal pathogen. (duke.edu)
- Aspergillus flavus has emerged as a predominant pathogen in patients with fungal sinusitis and fungal keratitis in several institutions worldwide. (elsevier.com)
- Aspergillus fumigatus is a ubiquitous opportunistic fungal pathogen that can cause severe infections in immunocompromised patients. (hki-jena.de)
- Aspergillus fumigatus is a major human fungal pathogen. (biomedcentral.com)
- Emergence of azole resistance in Aspergillus fumigatus and spread of a single resistance mechanism. (cdc.gov)
- Frequency and evolution of azole resistance in Aspergillus fumigatus associated with treatment failure. (cdc.gov)
- Aspergillus species and other molds in respiratory samples from patients with cystic fibrosis: a laboratory-based study with focus on Aspergillus fumigatus azole resistance. (cdc.gov)
- High prevalence of triazole resistance in Aspergillus fumigatus , especially mediated by TR/L98H, in a French cohort of patients with cystic fibrosis. (cdc.gov)
- Although this media can be used for the screening of any Aspergillus species for resistance to azoles, this procedure is written primarily for use with A. fumigatus . (cdc.gov)
- Azole resistance is a major concern for treatment of infections with Aspergillus fumigatus . (cdc.gov)
- Environmental resistance selection is a main route for Aspergillus spp. (cdc.gov)
- We investigated the presence of environmental hotspots for resistance selection in the Netherlands on the basis of the ability of A. fumigatus to grow and reproduce in the presence of azole fungicide residues. (cdc.gov)
- Preventing plant waste stockpiling or creating unfavorable conditions for A. fumigatus to grow in stockpiles might reduce environmental resistance burden. (cdc.gov)
- It is generally accepted that resistance can develop through patient treatment (patient route) and through exposure of A. fumigatus to azole fungicides in the environment (environmental route) ( 2 - 9 ). (cdc.gov)
- Azole resistance surveillance in Aspergillus fumigatus: beneficial or biased? (ru.nl)
- We believe that local resistance rates based on Aspergillus disease and/or risk group should be leading for decisions regarding empirical antifungal therapy in specific units. (ru.nl)
- Surveillance research may benefit from further standardization, which may be facilitated through the recently instituted International Society for Human and Animal Mycology (ISHAM) Aspergillus Resistance Surveillance Working Group. (ru.nl)
- 1 medical azoles (itraconazole, voriconazole, posaconazole, and isavuconazole) were defined as azole-resistant A. fumigatus and examined for resistance mechanisms, microsatellite-based phylogenetic relatedness, and growth rates following previously described methods ( 3 , 4 ). (cdc.gov)
- This observation is consistent with the estimated global prevalence of azole resistance in Aspergillus (3%-6%) and the predominance of environmental resistance mechanisms in azole-resistant A. fumigatus ( 1 , 5 ). (cdc.gov)
- Azole resistance of A. fumigatus warrants specific surveillance in hospitals treating immunocompromised patients. (cdc.gov)
- The results demonstrate that the WT distribution and ECVs for A. fumigatus and the mold-active triazoles were the same when determined by the CLSI or the EUCAST BMD method, which will be useful in resistance surveillance and is an important step toward the development of clinical breakpoints. (semanticscholar.org)
- Azole resistance in A. fumigatus is becoming an increasing problem, especially in patients with aspergillomas and those with low levels of itraconazole. (life-worldwide.org)
- Obtained results provide solid starting point for further studies that would include: (i) detailed chemical characterization of A. fumigatus CF, (ii) activity relationships and in vivo correlation with pathogenicity of prolonged pulmonary IA and (iii) possible use of biomolecules as diagnostic or therapeutic markers. (medsci.org)
- 200). Although bioaerosols can be generated through a range of operational procedures it has also been suggested that large numbers of Aspergillus fumigatus spores can be emitted from static compost piles through the action of natural air movements across the surface of the biodegrading material. (whiterose.ac.uk)
- Through the use of a portable wind tunnel apparatus researchers have determined the rate of emission of Aspergillus fumigatus spores as a result of air movement across the surface of the material (Taha et al. (whiterose.ac.uk)
- The aim of this work was to use controlled laboratory experiments using compost samples and Aspergillus fumigatus spores to determine the sporulation rate of Aspergillus fumigatus. (whiterose.ac.uk)
- A series of experiments were carried out using Aspergillus fumigatus spores on agar plates and small samples of green waste compost to determine the number of spores that could be generated by each existing spore over a seven day period. (whiterose.ac.uk)
- Although the data presented in this paper were determined from controlled laboratory experiments they show that it is possible for Aspergillus fumigatus to generate spores at a sufficient rate to allow the release of significant numbers of spores. (whiterose.ac.uk)
- Health effects associated with Aspergillus fumigatus The spores of Aspergillus fumigatus are very small. (moldbacteria.com)
- Such clothes should be handled with care since certain species of molds that attack clothes, such as species of Aspergillus , are opportunistic pathogens especially to people with weak immune system. (moldbacteriaconsulting.com)
- The second most frequent (approximately 15-20%) pathogenic species is Aspergillus flavus and to a lesser extent, Aspergillus niger and Aspergillus terreus. (elsevier.com)
- alternative approaches to diagnosis include detectable A. flavus or A. niger IgG antibodies, A. fumigatus IgE antibodies and biopsy/excision of lesions showing hyphae consistent with Aspergillus within a cavity. (life-worldwide.org)
- High prevalence of azole-resistant Aspergillus fumigatus in adults with cystic fibrosis exposed to itraconazole. (cdc.gov)
- The Aspergillus screening plates contain four quadrants with RPMI 1640 2% glucose agar: one growth control well with no antifungal powder, one voriconazole well, one itraconazole well, and one posaconazole well. (cdc.gov)
- Aspergillus fumigatus is the main causative agent of IA and investigation of its metabolites could provide valuable insight into virulence factor(s) associated with this organism. (medsci.org)
- Mice are the most extensively used model organism in research on A. fumigatus infections. (hki-jena.de)
- however, triazole-resistant A. fumigatus infections are increasingly reported worldwide and are associated with increased treatment failure and mortality. (cdc.gov)
- The majority (approximately 80%) of invasive Aspergillus infections is caused by Aspergillus fumigatus. (elsevier.com)
- If not efficiently removed in time, A. fumigatus conidia can germinate causing severe infections associated with high mortality rates. (hki-jena.de)
- When compared to other fungal allergens, diseases related with A. fumigatus allergens are on the rise, and it also complicates life-threatening infections in immunocompromised individuals such as cancer patients, HIV patients, and organ transplant recipients [ 5 ]. (springeropen.com)
- a much smaller number of infections may be attributed Malassezia, Zygomycetes, or Aspergillus pathogens. (medscape.com)
- Dr. Arun Balajee, a mycologist in the Marr lab, views a mold specimen that was initially believed to be Aspergillus fumigatus, a species that can cause life-threatening infections in transplant patients. (fredhutch.org)
- IMSEAR at SEARO: Perinephric abscess due to Aspergillus fumigatus. (who.int)
- Genome analysis showed that only one member of each subfamily is present in A. fumigatus, PMT1, PMT2 and PMT4. (archives-ouvertes.fr)
- This copy of the genome of Aspergillus fumigatus was obtained from AspGD . (doe.gov)
- FINDINGS: Five different bone substitutes (demineralized bone matrix, anorganic bovine bone, ß-tricalcium phosphate, synthetic nano-hydroxyapatite, and synthetic hydroxyapatite), commonly used in sinus floor augmentation procedures, were inoculated with conidia suspensions of A. fumigatus and incubated at 37 °C for 4 and 8 h, in standardized conditions. (units.it)
- A controlled determination of the effect of inhibitors associated with sampling indoor air and the ability of internal standard controls to detect inhibition was carried out by filter collection of air samples followed by spiking of the filters with green fluorescent protein-expressing Aspergillus fumigatus conidia. (cdc.gov)
- Ascomycetes are the perfect stages of molds like Aspergillus and Penicillium . (latesting.com)
- Aspergillus is a group of molds, only a few of which can cause illness in humans and animals. (fredhutch.org)
- Clonal expansion and Emergence of eEnvironmental multiple-triazole-resistant Aspergillus fumigatus strains carrying the TR(34)/L98H mutations in the cyp51A gene in India. (cdc.gov)
- The far more frequent microbe fungal diseases are Aspergillus spp. (springeropen.com)
- The patients with strong precipitin reaction showed high serum IgG levels against A. fumigatus antigens. (ias.ac.in)
- We assayed for the presence of serum anti-A. fumigatus IgG in bronchiectasis patients from a tertiary hospital in south Brazil and evaluated the relationship with clinical outcome. (bvsalud.org)
- The key diagnostic tests are serum Aspergillus IgG testing, also known as Aspergillus precipitins, and radiology showing one or more cavities or nodules. (life-worldwide.org)
- Evaluating patients suspected of having lung disease caused by Aspergillus fumigatus. (mghlab.com)
- Anti-Aspergillus fumigatus IgG in patients with bronchiectasis and its relationship with clinical outcome. (bvsalud.org)
- Asthmatics and cystic fibrosis patients are allergic to A. fumigatus . (springeropen.com)
- Our modern immunoinformatic research shows ITLKLLHRYSYKLAG, KLVLRAFPNHFRGDS, RYSYKLAGVNQVDVV, GKSFELNQAARAVTQ, and LHRYSYKLAGVNQVD epitopes could bind to MHC-II HLA allelic determinants and can initiate immunogenic response in patients affected by Aspergillus fumigatus . (springeropen.com)
- As described ( 3 ), we screened every A. fumigatus isolate recovered from respiratory specimens from patients with probable or proven IA in our hospital in Paris, France, during January 2012-December 2014. (cdc.gov)
- Sputum culture positive rates are ~25% and Aspergillus PCR is more sensitive, but many patients are still negative. (life-worldwide.org)
- Sarma, P. U. (1990) Studies on Aspergillus fumigatus antigens for serodiagnosis Serodiagnosis and Immunotherapy in Infectious Disease, 4 (3). (ias.ac.in)
- Previous work performed at our laboratory showed that the synthesis of immunodominant antigens from Aspergillus spp. (mediu.edu.my)
- A. fumigatus sensitisation is related to poor lung function. (ox.ac.uk)
- If you have emphysema , tuberculosis , or advanced sarcoidosis , lung cavities can be infected with Aspergillus . (healthline.com)
- With careful selection and standardization of test conditions it is possible to generate reproducible in-vitro susceptibility data for Aspergillus spp. (semanticscholar.org)
- Members of protein O-mannosyltransferase family in Aspergillus fumigatus differentially affect growth, morphogenesis and viability. (archives-ouvertes.fr)
- We investigated the frequency of filamentous fungal culture and IgE sensitisation to Aspergillus fumigatus and the relationship to clinical outcomes in COPD subjects. (ox.ac.uk)
- While some skeptics initially argued that the species was likely a mutation of A. fumigatus specific to the center - perhaps due to a unique combination of drug therapies - Balajee and colleagues have now identified A. lentulus at several different clinical centers in the United States, Japan and Australia. (fredhutch.org)
- In individuals with atopic asthma or cystic fibrosis, allergy is caused by a hypersensitivity response to Aspergillus allergens. (springeropen.com)
- Aspergillus fumigatus can cause a serious reaction in some people, especially those with asthma or cystic fibrosis . (healthline.com)
- Despite the fact that all PMTs are transmembrane proteins with conserved peptide motifs, the phenotype of each PMT deletion mutant was very different in A. fumigatus. (archives-ouvertes.fr)
- Protein database like NCBI GenBank, EMBL, and DDBJ was deployed for checking proteins of A. fumigatus with various accession numbers/GenBank ID. (springeropen.com)
- In sum, the zinc transport proteins encoded by zrfA and zrfB are members of a zinc uptake system of A. fumigatus that operates mainly under acid, zinc-limiting conditions. (mediu.edu.my)
- Tokios yra galvenių ( Aspergillus ), pelėjūnų ( Penicillium ) sporos (3 ir 4 pav). (alergo.lt)
- Worldwide emergence of azole-resistant Aspergillus fumigatus since the late 2000s threatens human health ( 1 ). (cdc.gov)
- Genetic replacement of the crzA locus in A. fumigatus resulted in a strain with significant defects in conidial germination, polarized hyphal growth, cell wall structure, and asexual development that are similar to but with differences from defects seen in the A. fumigatus DeltacnaA (calcineurin A) strain. (duke.edu)
- Although A. fumigatus occurs in areas with widely different climates and environments, it displays low genetic variation and a lack of population genetic differentiation on a global scale. (ensembl.org)
- Here, we show that Aspergillus fumigatus possesses three ido genes that are expressed under conditions of hypoxia or tryptophan abundance. (olmdiagnostics.com)
- By compiling a list of known regulatory interactions for iron homeostasis in A. fumigatus and softly integrating them during network inference, we are able to predict new interactions between transcription factors and target genes. (biomedcentral.com)
- In this work, we show that zrfA and zrfB are two of the genes that encode membrane zinc transporters from A. fumigatus in this system. (mediu.edu.my)
- We recently showed that when activated, these core PKS genes from A. fumigatus and N. fischeri can synthesize a prenylated, tricyclic polyketide neosartoricin 11 . (cdc.gov)
- To examine the activities of the conserved core PKS genes from dermatophytes, we cloned the clusters using yeast recombination 12 followed by expression in Aspergillus nidulans ( Figure S1, Supplementary Information ). (cdc.gov)
- A long and exhaustive investigation of the mold's genes by Balajee and Marr, with technical support from Jennifer Gribskov and University of Washington computational biologist Dr. David Nickle, proved the lab had uncovered a new species - Aspergillus lentulus. (fredhutch.org)
- When integrated into the model Aspergillus nidulans host, a structurally related compound neosartoricin B was formed, suggesting a possible role of this compound in the pathogenesis of these strains. (cdc.gov)
- Calcineurin target CrzA regulates conidial germination, hyphal growth, and pathogenesis of Aspergillus fumigatus. (duke.edu)
- Although A. fumigatus is not a phytopathogen and thus not a target for fungicide application, many azole fungicides show in vitro activity against A. fumigatus ( 12 , 13 ). (cdc.gov)
- Petkovic, L.D. In vitro Protease Inhibition and Cytotoxicity of Aspergillus fumigatus Biomolecules Secreted under Long-Term Aerated Conditions. (medsci.org)
- The aim of the present investigation is to evaluate in vitro eventual differences in the ability of Aspergillus fumigatus to colonize different bone grafting materials and grow on them as biofilm. (units.it)
- Biosynthesis of A. fumigatus low molecular weight proteinaceous products (from 6.4 to 15.4 kDa) was observed after 6 days of growth under aerated and alkaline conditions. (medsci.org)
- The noninvasive form called Aspergillus mycetoma occurs mostly in healthy people. (bvsalud.org)
- Despite the limitations the original intention of the work was to attempt to establish the long term applicability of the fugitive release rates for Aspergillus fumigatus from green waste compost quoted in the literature and it has achieved this objective. (whiterose.ac.uk)
- Aspergillus fumigatus , a saprotroph widespread in nature, is typically found in soil and decaying organic matter, such as compost heaps, where it plays an essential role in carbon and nitrogen recycling. (ensembl.org)
- Conclusions: Netrin-1 attenuates inflammation in C57BL/6 mice infected with A. fumigatus, and it may play this role via the receptor A2BAR. (mssm.edu)
- I looked at them in many classical mycology ways and it looked very much like A. fumigatus except there were subtle differences. (fredhutch.org)