Aspergillus fumigatus: A species of imperfect fungi from which the antibiotic fumigatin is obtained. Its spores may cause respiratory infection in birds and mammals.Aspergillus: A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.Aspergillosis: Infections with fungi of the genus ASPERGILLUS.Aspergillus nidulans: A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.Aspergillus niger: An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.Aspergillus flavus: A species of imperfect fungi which grows on peanuts and other plants and produces the carcinogenic substance aflatoxin. It is also used in the production of the antibiotic flavicin.Aspergillosis, Allergic Bronchopulmonary: Hypersensitivity reaction (ALLERGIC REACTION) to fungus ASPERGILLUS in an individual with long-standing BRONCHIAL ASTHMA. It is characterized by pulmonary infiltrates, EOSINOPHILIA, elevated serum IMMUNOGLOBULIN E, and skin reactivity to Aspergillus antigen.Spores, Fungal: Reproductive bodies produced by fungi.Pulmonary Aspergillosis: Infections of the respiratory tract with fungi of the genus ASPERGILLUS. Infections may result in allergic reaction (ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS), colonization in pulmonary cavities as fungus balls (MYCETOMA), or lead to invasion of the lung parenchyma (INVASIVE PULMONARY ASPERGILLOSIS).Antifungal Agents: Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.Gliotoxin: A fungal toxin produced by various species of Trichoderma, Gladiocladium fimbriatum, Aspergillus fumigatus, and Penicillium. It is used as an immunosuppressive agent.Itraconazole: A triazole antifungal agent that inhibits cytochrome P-450-dependent enzymes required for ERGOSTEROL synthesis.Hyphae: Microscopic threadlike filaments in FUNGI that are filled with a layer of protoplasm. Collectively, the hyphae make up the MYCELIUM.Lung Diseases, Fungal: Pulmonary diseases caused by fungal infections, usually through hematogenous spread.Antigens, Fungal: Substances of fungal origin that have antigenic activity.Fungal Proteins: Proteins found in any species of fungus.Invasive Pulmonary Aspergillosis: Lung infections with the invasive forms of ASPERGILLUS, usually after surgery, transplantation, prolonged NEUTROPENIA or treatment with high-doses of CORTICOSTEROIDS. Invasive pulmonary aspergillosis can progress to CHRONIC NECROTIZING PULMONARY ASPERGILLOSIS or hematogenous spread to other organs.Drug Resistance, Fungal: The ability of fungi to resist or to become tolerant to chemotherapeutic agents, antifungal agents, or antibiotics. This resistance may be acquired through gene mutation.TriazolesAmphotericin B: Macrolide antifungal antibiotic produced by Streptomyces nodosus obtained from soil of the Orinoco river region of Venezuela.Azoles: Five membered rings containing a NITROGEN atom.Echinocandins: Cyclic hexapeptides of proline-ornithine-threonine-proline-threonine-serine. The cyclization with a single non-peptide bond can lead them to be incorrectly called DEPSIPEPTIDES, but the echinocandins lack ester links. Antifungal activity is via inhibition of 1,3-beta-glucan synthase production of BETA-GLUCANS.DNA, Fungal: Deoxyribonucleic acid that makes up the genetic material of fungi.Fungi: A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.Antibodies, Fungal: Immunoglobulins produced in a response to FUNGAL ANTIGENS.Gene Expression Regulation, Fungal: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Mycological Typing Techniques: Procedures for identifying types and strains of fungi.Ergot Alkaloids: Alkaloids originally isolated from the ergot fungus Claviceps purpurea (Hypocreaceae). They include compounds that are structurally related to ergoline (ERGOLINES) and ergotamine (ERGOTAMINES). Many of the ergot alkaloids act as alpha-adrenergic antagonists.Drug Resistance, Multiple, Fungal: The ability of fungi to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance phenotype may be attributed to multiple gene mutations.Neuroaspergillosis: Infections of the nervous system caused by fungi of the genus ASPERGILLUS, most commonly ASPERGILLUS FUMIGATUS. Aspergillus infections may occur in immunocompetent hosts, but are more prevalent in individuals with IMMUNOLOGIC DEFICIENCY SYNDROMES. The organism may spread to the nervous system from focal infections in the lung, mastoid region, sinuses, inner ear, bones, eyes, gastrointestinal tract, and heart. Sinus infections may be locally invasive and enter the intracranial compartment, producing MENINGITIS, FUNGAL; cranial neuropathies; and abscesses in the frontal lobes of the brain. (From Joynt, Clinical Neurology, 1998, Ch 27, pp62-3)Mannans: Polysaccharides consisting of mannose units.Genes, Fungal: The functional hereditary units of FUNGI.Mycology: The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.Mycotoxins: Toxic compounds produced by FUNGI.Mycelium: The body of a fungus which is made up of HYPHAE.Air Microbiology: The presence of bacteria, viruses, and fungi in the air. This term is not restricted to pathogenic organisms.Pyrimidines: A family of 6-membered heterocyclic compounds occurring in nature in a wide variety of forms. They include several nucleic acid constituents (CYTOSINE; THYMINE; and URACIL) and form the basic structure of the barbiturates.Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.MycosesEurotiales: An order of fungi in the phylum ASCOMYCOTA characterized by the presence of well defined peridia and cleistothecial asci. Notable anamorphs (mitosporic forms) of Eurotiales include PENICILLIUM and ASPERGILLUS.beta-Glucans: Glucose polymers consisting of a backbone of beta(1->3)-linked beta-D-glucopyranosyl units with beta(1->6) linked side chains of various lengths. They are a major component of the CELL WALL of organisms and of soluble DIETARY FIBER.Penicillium: A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.Peptides, Cyclic: Peptides whose amino and carboxy ends are linked together with a peptide bond forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS. Some of them are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL).Aflatoxins: Furano-furano-benzopyrans that are produced by ASPERGILLUS from STERIGMATOCYSTIN. They are structurally related to COUMARINS and easily oxidized to an epoxide form to become ALKYLATING AGENTS. Members of the group include AFLATOXIN B1; aflatoxin B2, aflatoxin G1, aflatoxin G2; AFLATOXIN M1; and aflatoxin M2.Animals, Outbred Strains: Animals that are generated from breeding two genetically dissimilar strains of the same species.Aspergillus ochraceus: An imperfect fungus that produces ochratoxins and contaminates EDIBLE GRAIN and coffee beans.Eye Infections, Fungal: Infection by a variety of fungi, usually through four possible mechanisms: superficial infection producing conjunctivitis, keratitis, or lacrimal obstruction; extension of infection from neighboring structures - skin, paranasal sinuses, nasopharynx; direct introduction during surgery or accidental penetrating trauma; or via the blood or lymphatic routes in patients with underlying mycoses.Candida albicans: A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).Glucan Endo-1,3-beta-D-Glucosidase: An endocellulase with specificity for the hydrolysis of 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans including laminarin, paramylon, and pachyman.Siderophores: Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)Rhizopus: A genus of zygomycetous fungi of the family Mucoraceae, order MUCORALES, a common saprophyte and facultative parasite of mature fruits and vegetables. It may cause cerebral mycoses in diabetes and cutaneous infection in severely burned patients.Immunocompromised Host: A human or animal whose immunologic mechanism is deficient because of an immunodeficiency disorder or other disease or as the result of the administration of immunosuppressive drugs or radiation.Allergens: Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Fungal Polysaccharides: Cell wall components constituting a polysaccharide core found in fungi. They may act as antigens or structural substrates.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Neosartorya: A genus of ascomycetous fungi in the family Trichocomaceae, order EUROTIALES. Some species can cause opportunistic infections in humans, similar to its anamorph ASPERGILLUS.Lipopeptides: Compounds consisting of a short peptide chain conjugated with an acyl chain.Genome, Fungal: The complete gene complement contained in a set of chromosomes in a fungus.Granulomatous Disease, Chronic: A defect of leukocyte function in which phagocytic cells ingest but fail to digest bacteria, resulting in recurring bacterial infections with granuloma formation. When chronic granulomatous disease is caused by mutations in the CYBB gene, the condition is inherited in an X-linked recessive pattern. When chronic granulomatous disease is caused by CYBA, NCF1, NCF2, or NCF4 gene mutations, the condition is inherited in an autosomal recessive pattern.Chitin: A linear polysaccharide of beta-1->4 linked units of ACETYLGLUCOSAMINE. It is the second most abundant biopolymer on earth, found especially in INSECTS and FUNGI. When deacetylated it is called CHITOSAN.Antigens, Plant: Substances found in PLANTS that have antigenic activity.Sterol 14-Demethylase: An NADPH-dependent P450 enzyme that plays an essential role in the sterol biosynthetic pathway by catalyzing the demethylation of 14-methyl sterols such as lanosterol. The enzyme acts via the repeated hydroxylation of the 14-methyl group, resulting in its stepwise conversion into an alcohol, an aldehyde and then a carboxylate, which is removed as formic acid. Sterol 14-demethylase is an unusual cytochrome P450 enzyme in that it is found in a broad variety of organisms including ANIMALS; PLANTS; FUNGI; and protozoa.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Immunoglobulin E: An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).Sterigmatocystin: A carcinogenic mycotoxin produced in high yields by strains of the common molds, Aspergillus versicolor, A. nidulans, and an unidentified species of Bipolaris. It causes necrosis of the liver and kidney and has an inhibitory effect on orotic acid incorporation into nuclear RNA.Ergosterol: A steroid of interest both because its biosynthesis in FUNGI is a target of ANTIFUNGAL AGENTS, notably AZOLES, and because when it is present in SKIN of animals, ULTRAVIOLET RAYS break a bond to result in ERGOCALCIFEROL.Mitosporic Fungi: A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.Environmental Microbiology: The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.Fusarium: A mitosporic Hypocreales fungal genus, various species of which are important parasitic pathogens of plants and a variety of vertebrates. Teleomorphs include GIBBERELLA.Scedosporium: A mitosporic fungal genus previously called Monosporium. Teleomorphs include PSEUDALLESCHERIA.6-Phytase: An enzyme that catalyzes the conversion of myo-inositol hexakisphosphate and water to 1L-myo-inositol 1,2,3,4,5-pentakisphosphate and orthophosphate. EC 3.1.3.26.Glucan 1,4-alpha-Glucosidase: An enzyme that catalyzes the hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of polysaccharide chains with the release of beta-glucose. It is also able to hydrolyze 1,6-alpha-glucosidic bonds when the next bond in sequence is 1,4.Cytochrome P-450 Enzyme System: A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Claviceps: A genus of ascomycetous fungi, family Clavicipitaceae, order Hypocreales, parasitic on various grasses (POACEAE). The sclerotia contain several toxic alkaloids. Claviceps purpurea on rye causes ergotism.Emericella: A genus in the family Trichocomaceae, order EUROTIALES. The anamorph is ASPERGILLUS.Candida: A genus of yeast-like mitosporic Saccharomycetales fungi characterized by producing yeast cells, mycelia, pseudomycelia, and blastophores. It is commonly part of the normal flora of the skin, mouth, intestinal tract, and vagina, but can cause a variety of infections, including CANDIDIASIS; ONYCHOMYCOSIS; vulvovaginal candidiasis (CANDIDIASIS, VULVOVAGINAL), and thrush (see CANDIDIASIS, ORAL). (From Dorland, 28th ed)Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

Association of a myosin immunoanalogue with cell envelopes of Aspergillus fumigatus conidia and its participation in swelling and germination. (1/1653)

A myosin immunoanalogue was identified in conidia of Aspergillus fumigatus by Western blotting, indirect immunofluorescence assay, and gold immunoelectron microscopy with two different antimyosin antibodies. The distribution pattern of this protein was followed during the early stages of germination. A single 180-kDa polypeptide, detected predominantly in a cell envelope extract, was found to cross-react with monoclonal and polyclonal antibodies raised against vertebrate muscle myosin. Immunoelectron microscopy permitted precise localization of this polypeptide, indicating that myosin analogue was mainly distributed along the plasma membrane of resting and swollen conidia. In germinating conidia, indirect immunofluorescence microscopy revealed myosin analogue at the periphery of germ tubes, whereas actin appeared as dispersed punctate structures in the cytoplasm that were more concentrated at the site of germ tube emergence. A myosin ATPase inhibitor, butanedione monoxime, greatly reduced swelling and blocked germination. In contrast, when conidia were treated with cytochalasin B, an inhibitor of actin polymerization, swelling was not affected and germination was only partially reduced. Butanedione monoxime-treated conidia showed accumulation of cytoplasmic vesicles and did not achieve cell wall reorganization, unlike swollen conidia. Collectively, these results suggest an essential role for this myosin analogue in the deposition of cell wall components during germination of A. fumigatus conidia and therefore in host tissue colonization.  (+info)

In vitro and in vivo activities of NS-718, a new lipid nanosphere incorporating amphotericin B, against Aspergillus fumigatus. (2/1653)

We evaluated the in vitro and in vivo potencies of a new lipid nanosphere that incorporates amphotericin B (AmB), NS-718, against Aspergillus fumigatus. The in vitro activity of NS-718 (the MIC at which 90% of strains are inhibited [MIC90], 0.25 microgram/ml) against 18 isolates of A. fumigatus was similar to that of deoxycholate AmB (D-AmB; Fungizone; MIC90, 0.25 microgram/ml), but NS-718 was more potent than liposomal AmB (L-AmB; AmBi-some; MIC90, 1.0 microgram/ml). The in vivo efficacy of NS-718 in a rat model of invasive pulmonary aspergillosis was compared with those of D-AmB and L-AmB. A low dose (1 mg/kg of body weight) of L-AmB was ineffective (survival rate, 0%), although equivalent doses of D-AmB and NS-718 were more effective (survival rate, 17%). However, a higher dose of NS-718 (3 mg/kg) was more effective (survival rate, 100%) than equivalent doses of D-AmB and L-AmB (survival rate, 0%). To explain these differences, pharmacokinetic studies showed higher concentrations of AmB in the plasma of rats treated with NS-718 than in the plasma of those treated with D-AmB. Our results suggest that NS-718, a new preparation of AmB, is a promising antifungal agent with activity against pulmonary aspergillosis.  (+info)

Amphotericin B- and fluconazole-resistant Candida spp., Aspergillus fumigatus, and other newly emerging pathogenic fungi are susceptible to basic antifungal peptides. (3/1653)

The present study shows that a number of basic antifungal peptides, including human salivary histatin 5, a designed histatin analog designated dhvar4, and a peptide from frog skin, PGLa, are active against amphotericin B-resistant Candida albicans, Candida krusei, and Aspergillus fumigatus strains and against a fluconazole-resistant Candida glabrata isolate.  (+info)

Early diagnosis of central nervous system aspergillosis with combination use of cerebral diffusion-weighted echo-planar magnetic resonance image and polymerase chain reaction of cerebrospinal fluid. (4/1653)

We treated a patient diagnosed as central nervous system (CNS) aspergillosis with the combined use of cerebral diffusion-weighted echo-planar magnetic resonance imaging (DWI) and polymerase chain reaction of the cerebrospinal fluid (CSF-PCR). DWI, a cutting-edge imaging modality to reveal the earliest changes of cerebral infarction, detected cerebral fungal embolization when the conventional computed tomographic scan and magnetic resonance imaging failed to reveal it. CSF-PCR demonstrated the presence of Aspergillus-specific DNA in the specimen, when the conventional examination and culture of CSF were nonspecific or negative. These diagnostic methods could be useful in the early diagnosis of CNS aspergillosis.  (+info)

Aspergillus meningitis: diagnosis by non-culture-based microbiological methods and management. (5/1653)

The performance of antibody detection, antigen detection, and Aspergillus genus-specific PCR for diagnosing Aspergillus meningitis was investigated with 26 cerebrospinal fluid (CSF) samples obtained from a single patient with proven infection caused by Aspergillus fumigatus. Immunoglobulin G antibodies directed against Aspergillus were not detected by enzyme-linked immunosorbent assay in CSF or serum. The antigen galactomannan was detected in the CSF 45 days before a culture became positive, and Aspergillus DNA was detected 4 days prior to culture. Decline of the galactomannan antigen titer in the CSF during treatment with intravenous and intraventricular amphotericin B and intravenous voriconazole corresponded with the clinical response to treatment.  (+info)

Contaminations occurring in fungal PCR assays. (6/1653)

Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed. The identities of all contaminants were specified by cycle sequencing and GenBank analysis. Twelve of 150 PCR assays that together included over 2,800 samples were found to be contaminated (3.3% of the negative controls were contaminated during the DNA extraction, and 4.7% of the PCR mixtures were contaminated during the amplification process). Contaminants were specified as Aspergillus fumigatus, Saccharomyces cerevisiae, and Acremonium spp. Further analysis showed that commercially available products like zymolyase powder or 10x PCR buffer may contain fungal DNA. In conclusion, the risk of contamination is not higher in fungal PCR assays than in other diagnostic PCR-based assays if general precautions are taken.  (+info)

Production of specific monoclonal antibodies to Aspergillus species and their use in immunohistochemical identification of aspergillosis. (7/1653)

Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining.  (+info)

Modulation of neutrophil-mediated activity against the pseudohyphal form of Candida albicans by granulocyte colony-stimulating factor (G-CSF) administered in vivo. (8/1653)

Renewed interest in neutrophil transfusions has emerged with the development and clinical use of granulocyte colony-stimulating factor (G-CSF). G-CSF not only increases neutrophil (polymorphonuclear leukocyte, PMNL) production but also modulates various physiological properties of PMNL. The effects of G-CSF on PMNL-mediated fungicidal activity were evaluated by administration of G-CSF (300 micrograms/day subcutaneously) to 5 healthy volunteers for 6 days. G-CSF significantly enhanced PMNL-mediated damage of Candida albicans pseudohyphae by 33% (P=.007) on day 2 and by 44% (P=.04) on day 6 at a 10:1 effector:target ratio. In contrast, the ability of PMNL to induce damage of hyphae from either Fusarium solani or Aspergillus fumigatus did not significantly change during the study period. These data demonstrate that G-CSF administered in vivo modulates PMNL-mediated fungicidal activity against the pseudohyphal form of C. albicans, thereby suggesting potential utility of G-CSF as a biologic response-modifying therapy in some opportunistic fungal infections.  (+info)

A. fumigatus strains and culture conditions: A. fumigatus strains used in this study are listed in Table 1. A. fumigatus strains were propagated at 37° on complete medium or minimal medium (MM) with 0.5 mm of one of the following nitrogen sources: sodium glutamate, ammonium tartrate, sodium nitrate, sodium nitrite, or hypoxanthine (Cove 1966). Uridine and uracil were added at a concentration of 5 mm when appropriate. Selection of A. fumigatus mutants unable to utilize nitrate as the sole nitrogen source was obtained by plating spores on MM containing ammonium tartrate and 100 mm sodium chlorate (Cove 1966). The nature of the mutation was assessed by growth on MM supplemented with different nitrogen sources, as previously described (Cove 1976). Selection of A. fumigatus mutants auxotrophic for uridine and uracil was achieved on MM containing 1 mg/ml 5-fluoroorotic acid plus uridine and uracil (dEnfert 1996). Liquid cultures used for DNA-mediated transformation and genomic DNA preparation were ...
Filamentous fungi represent classical examples for environmentally acquired human pathogens whose major virulence mechanisms are likely to have emerged long before the appearance of innate immune systems. In natural habitats, amoeba predation could impose a major selection pressure towards the acquisition of virulence attributes. To test this hypothesis, we exploited the amoeba Dicytostelium discoideum to study its interaction with Aspergillus fumigatus, two abundant soil inhabitants for which we found co-occurrence in various sites. Fungal conidia were efficiently taken up by D. discoideum, but ingestion was higher when conidia were devoid of the green fungal spore pigment DHN-melanin, in line with earlier results obtained for immune cells. Conidia were able to survive phagocytic processing and intracellular germination was initiated only after several hours of coincubation which eventually led to a lethal disruption of the host cell. Besides phagocytic interactions, both amoeba and fungus ...
Link to Pubmed [PMID] - 28677124. Mol. Microbiol. 2017 Sep;105(6):880-900. Aspergillus fumigatus, a ubiquitous human fungal pathogen, produces asexual spores (conidia), which are the main mode of propagation, survival and infection of this human pathogen. In this study, we present the molecular characterization of a novel regulator of conidiogenesis and conidial survival called MybA because the predicted protein contains a Myb DNA binding motif. Cellular localization of the MybA::Gfp fusion and immunoprecipitation of the MybA::Gfp or MybA::3xHa protein showed that MybA is localized to the nucleus. RNA sequencing data and a uidA reporter assay indicated that the MybA protein functions upstream of wetA, vosA and velB, the key regulators involved in conidial maturation. The deletion of mybA resulted in a very significant reduction in the number and viability of conidia. As a consequence, the ΔmybA strain has a reduced virulence in an experimental murine model of aspergillosis. RNA-sequencing and ...
We present the genome sequences of a new clinical isolate of the important human pathogen, Aspergillus fumigatus, A1163, and two closely related but rarely pathogenic species, Neosartorya fischeri NRRL181 and Aspergillus clavatus NRRL1. Comparative genomic analysis of A1163 with the recently sequenced A. fumigatus isolate Af293 has identified core, variable and up to 2% unique genes in each genome. While the core genes are 99.8% identical at the nucleotide level, identity for variable genes can be as low 40%. The most divergent loci appear to contain heterokaryon incompatibility (het) genes associated with fungal programmed cell death such as developmental regulator rosA. Cross-species comparison has revealed that 8.5%, 13.5% and 12.6%, respectively, of A. fumigatus, N. fischeri and A. clavatus genes are species-specific. These genes are significantly smaller in size than core genes, contain fewer exons and exhibit a subtelomeric bias. Most of them cluster together in 13 chromosomal islands, ...
TY - JOUR. T1 - A Conserved C-Terminal Domain of the Aspergillus fumigatus Developmental Regulator MedA Is Required for Nuclear Localization, Adhesion and Virulence. AU - Al Abdallah, Qusai. AU - Choe, Se In. AU - Campoli, Paolo. AU - Baptista, Stefanie. AU - Gravelat, Fabrice N.. AU - Lee, Mark J.. AU - Sheppard, Donald C.. PY - 2012/11/21. Y1 - 2012/11/21. N2 - MedA is a developmental regulator that is conserved in the genome of most filamentous fungi. In the pathogenic fungus Aspergillus fumigatus MedA regulates conidiogenesis, adherence to host cells, and pathogenicity. The mechanism by which MedA governs these phenotypes remains unknown. Although the nuclear import of MedA orthologues has been reported in other fungi, no nuclear localization signal, DNA-binding domain or other conserved motifs have been identified within MedA. In this work, we performed a deletion analysis of MedA and identified a novel domain within the C-terminal region of the protein, designated MedA346-557, that is ...
The production of toxins by A. fumigatus may help the fungus to colonize and invade the respiratory epithelium by modifying the natural clearance of the respiratory tract. Previous research has shown that A. fumigatus culture filtrate modifies the transepithelial resistance (Rt) and transepithelial potential differences (Vt) of HNEC, an in vitro model of the air-liquid interface of airway epithelium [11]. The aim of this study was to use HPLC and MS-MS to identify which toxins produced by A. fumigatus are responsible for these modifications. Our data suggest that verruculogen, which has never been implicated in invasive aspergillosis, is one of the probable candidates.. The fact that A. fumigatus produces a number of biologically active substances that slow ciliary beating, damage epithelium, and that may affect colonization of the airways has already been reported using culture explants [17]. Among these substances, such toxins as gliotoxin, fumagillin, and helvolic acid have been implicated in ...
Melanins, or melanin-like compounds, may play a role in the pathogenesis of a number of human fungal infections. This study investigated the production of melanin by the important opportunistic pathogen Aspergillus fumigatus. Conidia from A. fumigatus were harvested and treated with proteolytic enzymes, denaturant and hot, concentrated acid; this yielded dark particles which were similar in size and shape to the original propagules. Electron spin resonance spectroscopy revealed that the conidial-derived particles were stable free radicals consistent with an identification as melanin. Melanin particles were used to immunize BALB/c mice in order to produce a total of five anti-melanin monoclonal antibodies (mAbs). The latter mAbs were strongly reactive both with intact conidia and with extracted melanin particles by ELISA and immunofluorescence reactivity. Immunofluorescence labelling with the novel mAbs was used to examine the temporal expression of melanin during in vitro culture of A. fumigatus
TY - JOUR. T1 - Biosynthesis of β-(1→5)-galactofuranosyl chains of fungal-type and o-mannose-type galactomannans within the invasive pathogen aspergillus fumigatus. AU - Chihara, Yuria. AU - Tanaka, Yutaka. AU - Izumi, Minoru. AU - Hagiwara, Daisuke. AU - Watanabe, Akira. AU - Takegawa, Kaoru. AU - Kamei, Katsuhiko. AU - Shibata, Nobuyuki. AU - Ohta, Kazuyoshi. AU - Oka, Takuji. PY - 2020/1/1. Y1 - 2020/1/1. N2 - ABSTRACT The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes. Galactomannans comprise α-(1→2)-/α-(1→6)-mannan and β-(1→5)-/ β-(1→6)-galactofuranosyl chains. We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains. In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus. Two paralogs exist within A. ...
Land, C.J.; Sostarić, B.; Fuchs, R.; Lundström, H.; Hult, K., 1989: Intratracheal exposure of rats to Aspergillus fumigatus spores isolated from sawmills in Sweden
In this experiment, the OD value of different concentrations of Aspergillus fumigatus cultured for 48 hours was measured on a microplate reader using a 96-well plate. The absorbance-concentration curve was plotted according to the results, and the absorbance value was used instead of visual observation to quickly detect the growth of Aspergillus fumigatus. situation. The results showed that the OD value was between 0.05 and 0.06 when visually 50%, and 100% when the OD value was greater than 0.17. The use of the OD value can be a rapid method for observing the growth of Aspergillus fumigatus, which has the advantages of high efficiency, accuracy and high throughput compared with the visual method.. ...
The opportunistic fungal pathogen Aspergillus fumigatus is the causative agent of more than 90% of all Invasive Aspergillosis (IA) infections diagnosed in immun...
While azole drugs targeting the biosynthesis of ergosterol are effective antifungal agents, their extensive use has led to the development of resistant organisms. Infections involving azole resistant forms of the filamentous fungus Aspergillus fumigatus are often associated with genetic changes in the cyp51A gene encoding the lanosterol α14 demethylase target enzyme. Both a sequence duplication in the cyp51A promoter (TR34) as well as a substitution mutation in the coding sequence (L98H) are required for full expression of azole resistance. A mechanism commonly observed in pathogenic yeast such as Candida albicans involves gain-of-function mutations in transcriptional regulatory proteins that induce expression of ATP-binding cassette (ABC) transporter encoding genes. We and others have found that an ABC transporter protein called Cdr1B (here referred to as AbcG1) is required for wild-type azole resistance in A.fumigatus Here we test the genetic relationship between the TR34 L98H allele of ...
Aspergillus fumigatus ATCC ® 1022D-2™ Designation: Genomic DNA from Aspergillus fumigatus Strain 118 [ATCC ® 1022™] Application:
Aspergillus fumigatus is the most common airborne fungal pathogen of humans, causing life-threatening invasive disease in immunocompromised patients. The limitations of therapeutic intervention are reflected in mortality rates, dependent primarily on the immune status of the host, of up to 90% (1, 2). The importance of the host immune status has been underlined by the lack of identified specific virulence attributes in A. fumigatus (1, 3-6). Limiting access to essential nutrients is an often overlooked aspect of innate immunity (7). Iron is essential for most organisms, suggesting that its acquisition in vivo may be required for A. fumigatus to cause disease (8-10). Iron uptake systems are often required for bacterial and yeast virulence (11, 12).. Fungi have evolved various strategies, often used in parallel, to acquire iron. These include two high affinity uptake mechanisms, reductive iron assimilation, and siderophore-assisted iron mobilization (8-10). Siderophores are low molecular mass, ...
Sensitization to Aspergillus fumigatus as a risk factor for bronchiectasis in COPD Stephanie Everaerts,1,2 Katrien Lagrou,3,4 Adriana Dubbeldam,5 Natalie Lorent,1 Kristina Vermeersch,2 Erna Van Hoeyveld,3 Xavier Bossuyt,3,4 Lieven J Dupont,1,2 Bart M Vanaudenaerde,2 Wim Janssens1,2 1Department of Respiratory Diseases, University Hospitals Leuven, 2Laboratory of Respiratory Diseases, Department of Clinical and Experimental Medicine, KU Leuven, 3Department of Laboratory Medicine, University Hospitals Leuven, 4Department of Microbiology and Immunology, KU Leuven, 5Department of Radiology, University Hospitals Leuven, Leuven, Belgium Background: Bronchiectasisâ chronic obstructive pulmonary disease (COPD) overlap presents a possible clinical phenotype of COPD, but it is unclear why it develops in a subset of patients. We hypothesized that sensitization to Aspergillus fumigatus (A fum) is associated with bronchiectasis in COPD and occurs more frequently in vitamin D-deficient patients.Methods: This
Aspergillus fumigatus is a thermotolerant human-pathogenic mold and the most common cause of invasive aspergillosis (IA) in immunocompromised patients. Its predominance is based on several factors most of which are still unknown. The thermotolerance of A. fumigatus is one of the traits which have been assigned to pathogenicity. It allows the fungus to grow at temperatures up to and above that of a fevered human host. To elucidate the mechanisms of heat resistance, we analyzed the change of the A. fumigatus proteome during a temperature shift from 30°C to 48°C by 2D-fluorescence difference gel electrophoresis (DIGE). To improve 2D gel image analysis results, protein spot quantitation was optimized by missing value imputation and normalization. Differentially regulated proteins were compared to previously published transcriptome data of A. fumigatus. The study was augmented by bioinformatical analysis of transcription factor binding sites (TFBSs) in the promoter region of genes whose corresponding
Modern medical technologies are repairing the human body in ways never imagined only a few years ago, but they are leaving an increasing population of patients who are newly susceptible to opportunistic pathogens. Invasive and chronic fungal infections have become a formidable clinical opponent, and foremost among them is Aspergillus fumigatus. Aspergillusfumigatus and Aspergillosis assembles chapters from a large and international contingent of experts in the field to explore every major aspect of A. fumigatus and how it kills so many patients. This volume offers the latest insights into the fundamental biology and pathogenesis of A. fumigatus and how it establishes disease, as well as the newest strategies for characterizing, diagnosing, and treating its spectrum of clinical infection. This valuable book is an instrumental resource for both scientists and clinicians tackling the current problems with Aspergillosis. It presents chapters on the species itself, including morphology and unique and
Purpose: Calcineurin orchestrates growth, stress responses and virulence in major pathogenic fungi including Aspergillus fumigatus responsible for life-threatening fungal infections worldwide. While these cellular regulatory functions of calcineurin make it an attractive antifungal target, the immunosuppressive effects of the currently available calcineurin inhibitors, FK506 and CsA, make it difficult to exploit the antifungal potential due to conservation of calcineurin in the host and the fungal pathogen. Critical molecular understanding of calcineurin-immunophilin-immunosuppressor complexes would facilitate the design of novel non-immunosuppressive CsA and FK506 analogs for fungal-specific targeting of calcineurin.. Methods: We solved the crystal structure of calcineurin-FK506-FKBP12 complex in A. fumigatus and using site-directed mutagenic approaches, we constructed several mutations in the CnaA catalytic subunit of calcineurin and FKBP12. To identify differences between the A. fumigatus ...
Link to Pubmed [PMID] - 23616783. Front Microbiol 2013;4:81. In Aspergillus fumigatus like in other filamentous ascomycetes, β-1,3-glucan constitutes a prominent cell wall component being responsible for rigidity of the cell wall structure. In filamentous fungi, softening of the cell wall is absolutely required during conidial germination and hyphal branching. Because of the central structure of β-1,3-glucans, it is expected that β-1,3-glucanases play a major role in cell wall softening. Based on in silico and experimental data, this review gives an overview of β-1,3-glucan modifying enzymes in A. fumigatus genome and their putative role during morphogenesis.. http://www.ncbi.nlm.nih.gov/pubmed/23616783 ...
2005. The Aspergillus nidulans phytochrome FphA represses sexual development in red light. Curr. Biol. 15:1833-1838. Casselton, L. A. 2002. Mate recognition in fungi. Heredity 88:142- 147. , J. P. Debeaupuis, J. Sarfati, J. Lortholary, P. Ribaud, P. Shah, M. Cornet, H. V. Thien, E. Gluckman, G. Bru ¨cker, and J. P. Latge´. 1998. Molecular typing of environmental and patient isolates of Aspergillus fumigatus from various hospital settings. J. Clin. Microbiol. 36:1494-1500. Debeaupuis, J. , J. Sarfati, V. Momany, T. Tanaka, T. Kumagai, K. Asai, M. Machida, W. C. Nierman, D. W. Denning, M. Caddick, M. Hynes, M. Paoletti, R. Fischer, B. Miller, P. Dyer, M. S. Sachs, S. A. Osmani, and B. W. Birren. 2005. Sequencing and comparative analysis of Aspergillus nidulans. Nature 438:1105-1115. , M. Christensen, A. H. Onions, J. I. Pitt, and R. A. Samson. 1985. Infrageneric taxa of Aspergillus, p. 55-62. In R. A. Samson and J. I. ), Advances in Penicillium and Aspergillus Systematics. Plenum Press, New ...
Aspergillus fumigatus is the main cause of invasive aspergillosis in immunocompromised patients, and only a limited number of drugs for treatment are available. A screening method for new antifungal compounds is urgently required, preferably an appro
Objective: To report the cases of 6 patients with fungus ball caused by Aspergillus fumigatus (aspergilloma) in the pleural cavity. Methods: Between 1980 and 2009, 391 patients were diagnosed with aspergilloma at the Santa Casa Hospital Complex in Porto Alegre, Brazil. The diagnosis of aspergilloma in the pleural cavity was made through imaging tests revealing effusion and pleural thickening with air-fluid level; direct mycological examination revealing septate hyphae, consistent with Aspergillus sp.; and positive culture for A. fumigatus in the surgical specimen from the pleural cavity. Results: Of the 391 patients studied, 6 (2%) met the established diagnostic criteria. The mean age of those 6 patients was 48 years (range, 29-66 years), and 5 (83%) were male. The most common complaints were cough, expectoration, and hemoptysis. Four patients (67%) had a history of tuberculosis that had been clinically cured. All of the patients were submitted to surgical removal of the aspergilloma, followed ...
Hyperspectral imaging (HSI) is a technique based on the combination of classical spectroscopy and conventional digital image processing. It is also well suited for the biological assays and quantitative real-time analysis since it provides spectral and spatial data of samples. The method grants detailed information about a sample by recording the entire spectrum in each pixel of the whole image. We applied HSI to quantify the constituent pH variation in a single infected apoptotic monocyte as a model system. Previously, we showed that the human-pathogenic fungus Aspergillus fumigatus conidia interfere with the acidification of phagolysosomes. Here, we extended this finding to monocytes and gained a more detailed analysis of this process. Our data indicate that melanised A. fumigatus conidia have the ability to interfere with apoptosis in human monocytes as they enable the apoptotic cell to recover from mitochondrial acidification and to continue with the cell cycle. We also showed that this ability of A
could get genotypically -,,Jacques Meis: Yes.,,Christine Ginocchio: - with the resistance. Okay.,,Jacques Meis: At least in Europe. Im not sure what the epidemiology is here. Probably, you have also a lot of -,,Tom Chiller: We dont - we dont ...
Aspergillus fumigatus Basics Laboratory Metabolites Adverse health reactions Specific settings Diagnostic Bibliography Basics A. fumigatus has been associated with each and every type of health problems linked to environmental moulds: irritation and inflammation, allergy, asthma, pneumonitis, toxic effects as well as a wide range of infections. Infections have been reported
Fungal keratitis is a kind of intractable and sight-threatening diseases. Spleen-tyrosine kinase (Syk) is a non-receptor tyrosine kinase, which plays an important role in the signaling pathway of the receptors. In the current study, we investigate the expression and function of Syk in human corneal epithelial cells with Aspergillus fumigatus (A. fumigatus) infection. Cultured telomerase-immortalized human corneal epithelial cells (THCEs) were treated with A. fumigatus hyphae with or without treatment of Syk inhibitors. Activation of Syk and the role of Syk in regulating inflammatory cytokines and chemokines expression were evaluated. The mRNA expression was determined by real time PCR, and protein activation was measured by western blotting. Syk protein was detected in THCEs, and its activation was enhanced after treatment of A. fumigatus hyphae. Expression of inflammatory cytokines (IL-1β and IL-6) and chemokines (IL-8 and CXCL1) mRNA were significantly increased after stimulation of A. fumigatus
Strong clinical and experimental evidence links qualitative and quantitative neutrophil deficiencies to fatal infections caused by A. fumigatus. Yet the role of the neutrophil in mediating the protection observed in normal hosts remains largely unknown. Recent studies indicate neutrophils from CXCR2-/- mice are unable to migrate toward chemokine gradients of KC and MIP-2, rendering these animals susceptible to fatal aspergillosis. Mice with a mutation in the gene encoding for the gp91phox component of the NADPH oxidase lack the ability to generate the reactive oxygen metabolites used by phagocytes in killing microbial pathogens, and these mice are also susceptible to invasive pulmonary aspergillosis. In this investigation of the innate response to A. fumigatus, CXCR2-/- and gp91-/- mice were used to mimic the qualitative and quantitative neutrophil defects that are known to predispose to invasive pulmonary aspergillosis, the most lethal form of Aspergillus diseases. By comparing the nature of ...
TY - JOUR. T1 - Posttraumatic invasive Aspergillus fumigatus wound infection. AU - Gettleman, Lynn K.. AU - Shetty, Avinash K.. AU - Prober, Charles G.. PY - 1999/8/1. Y1 - 1999/8/1. UR - http://www.scopus.com/inward/record.url?scp=0032782329&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0032782329&partnerID=8YFLogxK. U2 - 10.1097/00006454-199908000-00026. DO - 10.1097/00006454-199908000-00026. M3 - Article. C2 - 10462356. AN - SCOPUS:0032782329. VL - 18. SP - 745. EP - 747. JO - Pediatric Infectious Disease Journal. JF - Pediatric Infectious Disease Journal. SN - 0891-3668. IS - 8. ER - ...
In most individuals, when benign antigens are inhaled, the lung responds with a tolerogenic immunological response [13-15]. However, in diseases such as ABPA, tolerance is lost or not established, and patients become sensitized to the inhaled fungal antigen Aspergillus fumigatus. We have previously shown that peripheral CD11c + DCs and TSLP-DCs from ABPA patients induce robust Th2 cytokine responses from autologous CD4+ T-cells in an OX40L-dependent manner [7]. In addition, in our CF cohort, vitamin D deficiency was associated with ABPA. In vitro treatment with 1,25-(OH)2 vitamin D3 reduced the robust Th2 response in patients with ABPA [7]. Based on data from our observational study, we initiated a clinical trial to assess the safety and the immunological effects of supplemental vitamin D3 (cholecalciferol) in patients with CF and ABPA.. This Phase I trial was implemented to test the safety and efficacy of vitamin D supplementation in CF patients with documented evidence of Af seinsitization. ...
Four patients who had recently received kidney transplants became infected with Aspergillus fumigatus while receiving immunosuppressive therapy. Three were shown to have invasive pulmonary mycotic disease, and one of these had documented dissemination. A fourth patient had respiratory symptoms and fever and was found to have mycelial forms consistent with A. fumigatus in his sputum, verified by cultures. All four were effectively treated with amphotericin B in low, widely spaced doses. Early diagnosis was apparently the key to successful management of the invasive Aspergillus fumigatus infection in these patients. Discovery of mycelial forms in fresh preparations of sputa or bronchial washings is a valuable clue to active infections. Securing tissue by biopsy is warranted in those patients who develop a pulmonary infiltrate or cavity that is not otherwise causally explained. ...
Aspergillus fumigatus is the major filamentous fungal pathogen in humans. Invasive aspergillosis has up to a 90% mortality rate in particular patient population...
TY - JOUR. T1 - A role for the unfolded protein response (UPR) in virulence and antifungal susceptibility in Aspergillus fumigatus. AU - Richie, Daryl L.. AU - Hartl, Lukas. AU - Aimanianda, Vishukumar. AU - Winters, Michael S.. AU - Fuller, Kevin K.. AU - Miley, Michael D.. AU - White, Stephanie. AU - McCarthy, Jason W.. AU - Latgé, Jean Paul. AU - Feldmesser, Marta. AU - Rhodes, Judith C.. AU - Askew, David S.. PY - 2009/1/1. Y1 - 2009/1/1. N2 - Filamentous fungi rely heavily on the secretory pathway, both for the delivery of cell wall components to the hyphal tip and the production and secretion of extracellular hydrolytic enzymes needed to support growth on polymeric substrates. Increased demand on the secretory system exerts stress on the endoplasmic reticulum (ER), which is countered by the activation of a coordinated stress response pathway termed the unfolded protein response (UPR). To determine the contribution of the UPR to the growth and virulence of the filamentous fungal pathogen ...
BioAssay record AID 768982 submitted by ChEMBL: Antifungal activity against Aspergillus fumigatus MTCC 3376 at 30 degC after 72 hrs by microbroth dilution technique.
Aspergillus fumigatus ATCC ® MYA-3627™ Designation: FG 1432 Application: Antifungal susceptibility testing Ref Ref Emerging infectious disease research Biomedical Research and Development Material Respiratory research Opportunistic pathogen research
BioAssay record AID 114336 submitted by ChEMBL: Effective dose against Aspergillus fumigatus MTU6001 strain after iv administration in murine.
Aspergillus fumigatus IgM/IgG Antibody Detection An indirect EIA is designed for the detection of anti-galactomannan IgM/IgG antibodies in human serum or plasma. The antibodies are used as the markers for IA. Serological Profile of Invasive...
Glycosylphosphatidylinositol (GPI) represents a mechanism for the attachment of proteins to the plasma membrane found in all eukaryotic cells. GPI biosynthesis has been mainly studied in parasites, yeast, and mammalian cells. Aspergillus fumigatus, a
Specific surveys of the air for Aspergillus fumigatus were carried out in rural and urban situations over a 2-year period. Overall, low concentrations of spores were recorded with a higher incidence during the winter months. Counts in the open air and in a hospital ward showed similar fluctuations …
Purpose: We investigated the expression and function of thymic stromal lymphopoietin (TSLP) in telomerase-immortalized human corneal epithelial cells (THCEs) and telomerase-immortalized human stroma fibroblasts (THSFs) challenged by A. fumigatus hyphae and its relationship with Toll-like receptors (TLR).. Methods: We stimulated THCEs and THSFs with TLR2 Ligand zymosan, TLR4 ligand lipopolysaccharide (LPS), human recombinant TSLP or A. fumigatus hyphae for various periods, with or without the inhibition of TLR2, TLR4 or TSLP using monoclonal antibody or small interfering RNA previously. The release and expression of TLR2, TLR4, TSLP, IL-4, IL-8, IL-13 and TNF-α were measured by means of ELISA, quantitative RT-PCR or western blot.. Results: It was demonstrated that enhanced expression of TSLP, IL-4 and IL-13 was associated with the treatment of A. fumigatus hyphae in human corneal cell lines. Stimulation of THCEs and THSFs with TLR2 Ligand zymosan or TLR4 ligand lipopolysaccharide (LPS) induced ...
A recent study from the University of Manchester has shown that human cell lines can be used to study lung colonisation by the fungus Aspergillus fumigatus. The work, published in Nature Communications, was partly funded by an NC3Rs Fellowship to Dr Sara Gago. Exposure to Aspergillus fumigatus in the environment is constant but the fungus is normally cleared from the lungs. Patients with asthma or an immune defect, however, can often have difficulty clearing the pathogen leading to aspergillosis. The team at the University of Manchester have discovered a genetic mutation in humans linked to increased loads of fungal spores in the lungs. Many laboratories use mouse models to study aspergillosis, which can be associated with welfare concerns as they involve the development of severe lung disease and mild-to-moderate respiratory distress. Human cell-based models can offer a more relevant alternative; for example, the mutation discovered in this study is in a gene for a transcription factor not found in
Aspergillus fumigatus is a ubiquitously distributed filamentous fungus that has emerged as one of the most serious life-threatening pathogens in immunocompromised patients. The mechanisms for its pathogenicity are poorly understood. Here, we analyzed the proteome of dormant A. fumigatus conidia as the fungal entity having the initial contact with the host. Applying two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), we established a 2-D reference map of conidial proteins. By MALDI-TOF mass spectrometry, we identified a total number of 449 different proteins. We show that 57 proteins of our map are over-represented in resting conidia compared to mycelium. Enzymes involved in reactive oxygen intermediates (ROI) detoxification, pigment biosynthesis, and conidial rodlet layer formation were highly abundant in A. fumigatus spores and most probably account for their enormous stress resistance. Interestingly, pyruvate decarboxylase and alcohol dehydrogenase were detectable in dormant ...
Phagocytosis of a fungus spore. Coloured scanning electron micrograph (SEM) of a neutrophil (green) phagocytosing (engulfing and destroying) spores from the fungus Aspergillus fumigatus (yellow). Neutrophils are the most abundant white blood cell and are part of the bodys immune system. Aspergillus fumigatus is common in dust, soil, and on plants and plant products such as hay or grain. It can cause a number of different diseases in humans, including allergic disorder, respiratory infection and invasive disease. - Stock Image P266/0123
Guinea pigs (0.4-0.45 kg in weight) were obtained from the Animal Center of the College of Basic Sciences, Jilin University. The use of these animals adhered to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. A strain of A. fumigatus (JLMR054) that was isolated from a patient with fungal endophthalmitis was donated by the Fungal Department of the College of Basic Sciences, Jilin University. Using a 30-gauge needle and a 1.0-mL plastic syringe, 0.02 mL Aspergillus suspension (1.0 × 106 CFU/mL) was injected directly into the vitreous cavity of 30 guinea pigs at the pars plana, approximately 1.5 mm posterior to the limbus. Voriconazole and liposomal Amp-B were supplied in powder form by Livzon Pharmaceutical Group, Inc. (Zhuhai, China) and New Pioneer, Inc. (Shanghai, China), respectively. The powder was suspended in 100% dimethylsulfoxide prior to use. The animals were randomly divided into three groups. Group A (control group) received an intravitreally administered ...
Invasive aspergillosis (IA), often caused by Aspergillus fumigatus, is an important cause of death of immunocompromised patients. Several DNA-extraction methods and PCR assays are available for detecting Aspergillus fumigatus DNA in bronchoalveolar lavage (BAL) samples of patients with invasive aspergillosis. These methods are often time consuming and emphasize the need to develop a clinical relevant rapid DNA isolation assay that gives reliable results in a short time. We have developed a new and rapid method which yields results within six hours.This was achieved by combining high-speed cell disruption using a mechanical extraction procedure (FastPrep), with a real-time PCR assay based on TaqMan technology.A newly designed Aspergillus-fumigatus-specific probe and Aspergillus-fumigatus-specific primers were established. This combination also produces quantitative results by comparing the results with a DNA serial dilution used in the real-time PCR. BAL fluids and other material from 204 ...
Summary In the human pathogenic mold Aspergillus fumigatus, sexual identity is determined by the mating‐type idiomorphs MAT1‐1 and MAT1‐2 residing at the MAT ...
Results] We were able to observe 3,622 genes modulated in at least one timepoint in the mutant when compared to the wild type strain (3,211 and 411 at 10 and 30 minutes, respectively). Decreased mRNA abundance in the ΔcrzA was seen for genes encoding calcium transporters, transcription factors and genes that could be directly or indirectly involved in calcium metabolism. Increased mRNA accumulation was observed for some genes encoding proteins involved in stress response. AfCrzA overexpression in A. fumigatus increases the expression of several of these genes. The deleted strain of one of these genes, AfRcnA, belonging to a class of endogenous calcineurin regulators, calcipressins, had more calcineurin activity after exposure to calcium and was less sensitive to menadione 30 μM, hydrogen peroxide 2.5 mM, EGTA 25 mM, and MnCl2 25 mM. We constructed deletion, overexpression, and GFP fusion protein for the closely related A. nidulans AnRcnA. GFP::RcnA was mostly detected along the germling, did ...
This page provides information on the status of the A. fumigatus Af293 genome. Data on this page are updated once a day. All the data displayed on this page are available in one or more files (Chromosomal Feature File; GO Annotations File) on the AspGD Download Data page. The Advanced Search tool can also be used to retrieve chromosomal features that match specific criteria. ...
Aspergillus fumigatus AFPAB1 is the ortholog of the Aspergillus oryzae cytoplasmic messenger ribonucleoprotein granules AOPAB1 that function to depress the initiation of translation during stress. A. fumigatus can regulate its cellular physiology in response to environmental stresses, but there has been no research on Pab1 in A. fumigatus. The associated gene afpab1 was replaced with a hygromycin-selectable marker to generate the strain Δafpab1. Phenotypic analysis showed that the Δafpab1 grew more weakly than the wild-type strain. Also the germination rate of Δafpab1 was decreased when exposed to oxidative stress. The morphology of Δafpab1 spores also showed great changes. The killing rate of Δafpab1 by RAW264.7 murine macrophage cells was increased, and the reactive oxygen species (ROS) scavenging ability of Δafpab1 was decreased. Pathogenicity testing showed that the deletion strain had decreased virulence. Therefore, we conclude that afpab1 activity is correlated with susceptibility to
Requires Fe2+ and ascorbate. Found in the fungus Aspergillus fumigatus. Both atoms of a dioxygen molecule are incorporated into verruculogen. Involved in the biosynthetic
Fumigatus isolates from India harboring TR34/ L98H mutations in the cyp51A gene, from soil samples of paddy fields, tea gardens, cotton trees, flower pots and
Aspergillus fumigatus is a medically important fungus, causing potentially life-threatening infections in patients with weakened immune systems. It is also a major cause of respiratory allergy, and it is implicated in asthma as well. The fungus has always been thought to lack the ability to reproduce sexually, but new discoveries by a multinational group of scientists indicate that the fungus has a number of characteristics of sex. The possible presence of sex in the species is highly significant because it may affect the way researchers study--and try to control--disease associated with the fungus. The team, headed by Paul Dyer at the University of Nottingham (UK), with lead researchers Mathieu Paoletti (University of Nottingham, UK) and Carla Rydholm (Duke University, USA), used a number of techniques to study the fungus. The genome of Aspergillus fumigatus has recently been sequenced, and investigation of the genome revealed the presence of a series of genes required for sexual reproduction. ...
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject-specific sections.
Activity on β-1,3-glucan (curdlan) shown for the Aspergillus fumigatus Sun4 protein; activity on laminarioligosaccharides shown for Aspergillus fumigatus Sun4 protein and Candida albicans Sun41 protein; transglycosylation activity reported in PMID 23508952 ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
Candida, Risk, Candida Glabrata, Prevalence, Aspergillus, Aid, Laboratories, Amphotericin B, Mutations, Aspergillus Fumigatus, Aspergillosis, and Patients
Uterine infections in three mares following repeated uterine manipulations and antibiotic therapy were caused by Aspergillus fumigatus in two cases and Candida albicans in the third. Two of the infections were noticed during dioestrus and one at oestrus and one mare produced a purulent vulval discharge. Large numbers of neutrophils, increased protein concentrations and the presence of fungal hyphae in flushings from the uterine lumen were indicative of active infection. The mycotic infections caused only transient aberrations of the oestrous cycle and recovery without treatment was completed within 23 days.. ...
Humane neutrophile Granulozyten spielen eine wichtige Rolle in der Immunabwehr invasiver Infektionen durch die humanpathogenen Pilze Candida albicans und Aspergillus fumigatus. Das Ziel der hier vorliegenden Arbeit bestand in einer Charakterisierung der Interaktion beider Pilzspezies mit neutrophilen Granulozyten, mit Fokussierung auf die unterschiedlichen Effektormechanismen dieser Zellen. C. albicans exprimiert eine Reihe von Aspartatproteasen, welche mit der Virulenz des Erregers assoziiert sind und zu Adhäsion, Gewebeinvasion und Immunevasion beitragen können. In dieser Arbeit wurde die Rolle der Aspartatproteasen Sap1-6, Sap9 und Sap10 in der Interaktion mit neutrophilen Granulozyten analysiert. Es konnte gezeigt werden, dass, im Gegensatz zu anderen Aspartatproteasen, das zelloberflächenassoziierte GPI-verankerte Enzym Sap9 einen maßgeblichen Einfluss auf die Erkennung von C. albicans durch neutrophile Granulozyten hat. SAP9-Expression ist erforderlich, um die gerichtete Motilität ...
1: Pandit H, Kale K, Yamamoto H, Thakur G, Rokade S, Chakraborty P, Vasudevan M, Kishore U, Madan T and Fichorova RN. Surfactant Protein D Reverses the Gene Signature of Transepithelial HIV-1 Passage and Restricts the Viral Transfer Across the Vaginal Barrier. Front Immunol. 2019, Mar 28;10:264.. 2: Shende R, Wong SSW, Rapole S, Beau R, Ibrahim-Granet O, Monod M, Gührs KH, Pal JK, Latgé JP, Madan T, Aimanianda V, Sahu A. Aspergillus fumigatus conidial metalloprotease Mep1p cleaves host complement proteins. J Biol Chem. 2018 Oct 5;293(40):15538-15555.. 3: Kumar A, Banerjee A, Singh D, Thakur G, Kasarpalkar N, Gavali S, Gadkar S, Madan T, Mahale SD, Balasinor NH, Sachdeva G. Estradiol: A Steroid with Multiple Facets. Horm Metab Res. 2018 Mar 22.. 4: Waghu FH, Joseph S, Ghawali S, Martis EA, Madan T, Venkatesh KV, Idicula-Thomas S. Designing Antibacterial Peptides with Enhanced Killing Kinetics. Front Microbiol. 2018 Feb 23;9:325.. 5: Sze Wah Wong S, Rani M, Dodagatta-Marri E, Ibrahim-Granet O, ...
Mutual Independence of Alkaline- and Calcium-mediated Signalling in Aspergillus fumigatus Refutes the Existence of a Conserved Druggable Signalling Nexus ...
Diagnoza alergijske bronhopulmonalne aspergiloze temelji na kliničnih, radioloških in imunoloških izslednih.[3] Za postavitev diagnoze ABPA se ugotavlja prisotnost glavnih in ostalih kriterijev. Glavni kriteriji so: anamneza astme, prisotnost pljučnih infiltratov, prisotnost eozinofilije v periferni krvi, prisotnost serumskih precipitinov za Aspergilus fumigatus, pozitivni kožni testi za aspergilusni antigen, povišani celokupni IgE v krvi, povišani specifični IgE in IgG za Aspergilus fumigatus, s pomočjo visokoločljivostne računalniške tomografije vidne centralne bronhiektazije.[2] ...
Voyage dans lAmérique meridionale (le Brazil, la Republique Argentine...Chili, Peru, &c.) pendant 1826 - 1833 par A. dOrbigny. 4 livr.51 pl.65 fig.1 1847 livr.89 p. ...
Review question We planned to review evidence about the effect of treatments to fight fungal infections which cause allergic bronchopulmonary aspergillosis in people with cystic fibrosis.. Background Allergic bronchopulmonary aspergillosis (ABPA) is an allergic lung reaction to a type of fungus (usually Aspergillus fumigatus) in some people with cystic fibrosis. It causes cough and wheezing and sometimes fever. If left untreated ABPA can lead to chronic lung damage. It is usually treated with a high dose of corticosteroids (also known as steroids). However, it has not been proven that corticosteroids can prevent lung function deteriorating in the long term. Also, long-term use of steroids is linked to some serious side effects. Treating the fungus which causes ABPA may be an alternative to using high doses of steroids to combat the allergic reaction. This is an update of a previously published review.. Search date The evidence is current to: 29 September 2016.. Study characteristics No trials ...
Allergic Bronchopulmonary Aspergillosis (ABPA): Symptoms ❗ Workup ❗ Diagnosis ❗ Treatment ❗ Complications ❗ Causes ❗ Epidemiology ❗ Incidence ❗ Prognosis ❗ Check at SYMPTOMA.com Allergic bronchopulmonary aspergillosis is a disease characterized by a hypersensitivity reaction to aspergillus fumigatus after its repeated inhalation and is most…
Caspofungin is a member of the echinocandin class of antifungal agents that inhibit the synthesis of β 1,3 glucan thus disrupting fungal cell wall structure and function. Exposure of the Aspergillus fumigatus cultures to caspofungin (0.01, 0.1 or 1.0 μ g/ml) resulted in a reduction in cell growth, but the production of the epipolythiodioxopiperazine toxin, gliotoxin, was comparable, or greater, in cultures exposed to caspofungin than untreated controls. Exposure of A. fumigatus hyphae to 1.0 μ g/ml caspofungin for 4 h resulted in the release of amino acids ( P 0.01), protein ( P 0.002) and gliotoxin ( P 0.02). Cultures of A. fumigatus incubated in the presence of caspofungin for 4 or 24 h demonstrated enhanced gliotoxin release ( P 0.04 and 0.03, respectively) and biosynthesis ( P 0.04 and 0.03, respectively) compared to that by control cultures. The results presented here indicate that exposure of A. fumigatus to caspofungin results in increased cell permeability and an increase in the ...
Allergic bronchopulmonary aspergillosis (ABPA) is a pulmonary disorder caused by a complex hypersensitivity response to antigens released by the fungus Aspergillus fumigatus. The management of ABPA includes two important aspects - institution of immunosuppressive therapy in the form of glucocorticoids to control the immunologic activity, and close monitoring for detection of relapses. Another possible target is to use antifungal agents to attenuate the fungal burden secondary to the fungal colonization in the airways. Oral corticosteroids are currently the treatment of choice for ABPA associated with bronchial asthma.They not only suppress the immune hyperfunction but are also anti-inflammatory. However, there is no data to guide the dose and duration of glucocorticoids and different regimens of glucocorticoids have been used in literature.Itraconazole, an oral triazole with relatively low toxicity, is active against Aspergillus spp. in vitro and in vivo. The activity of itraconazole against ...
This study describes a sudden death in an ostrich (Struthio camelus) with a big neck. Grossly, the air sacs were thickened. Yellow to white, round or coalescent material was scattered on the air sacs. However, the cervical air sac was normal. Histopathologically, multinucleated giant cells, heterophils, and macrophages had infiltrated the air sacs, and many hyphae were seen in the air sacs and on their surfaces. Pyogranulomatous inflammation with intralesional hyphae was observed throughout the lungs. Aspergillus fumigatus was identified microbiologically. In conclusion, the affected animal died because of the respiratory disturbance caused by mycotic airsacculitis and pneumonia.
Allergic bronchopulmonary aspergillosis (ABPA) is a condition characterised by an exaggerated response of the immune system (a hypersensitivity response) to the fungus Aspergillus (most commonly Aspergillus fumigatus). It occurs most often in patients with asthma or cystic fibrosis. Aspergillus spores are ubiquitous in soil and are commonly found in the sputum of healthy individuals. A. fumigatus is responsible for a spectrum of lung diseases known as aspergilloses. ABPA causes airway inflammation, leading to bronchiectasis-a condition marked by abnormal dilation of the airways. Left untreated, the immune system and fungal spores can damage sensitive lung tissues and lead to scarring. The exact criteria for the diagnosis of ABPA are not agreed upon. Chest X-rays and CT scans, raised blood levels of IgE and eosinophils, immunological tests for Aspergillus together with sputum staining and sputum cultures can be useful. Treatment consists of corticosteroids and antifungal medications. Almost all ...
Direct utilization of untreated oil palm trunk (OPT) for cellulases and xylanase production by Aspergillus fumigatus SK1 was conducted under solid-state fermentation (SSF). The highest activities of extracellular cellulases and xylanases were produced at 80% moisture level, initial pH 5.0, 1 × 108 spore/g (inoculum) with 125 μm of OPT as sole carbon source. The cellulases and xylanase activities obtained were 54.27, 3.36, 4.54 and 418.70 U/g substrates for endoglucanase (CMCase), exoglucanase (FPase), β-glucosidase and xylanase respectively. The crude cellulases and xylanase required acidic condition to retain their optimum activities (pH 4.0). Crude cellulases and xylanase were more stable at 40°C compared to their optimum activities conditions (60°C for FPase and 70°C for CMCase, β-glucosidase and xylanase). SDS-PAGE and zymogram analysis showed that Aspergillus fumigatus SK1 could secrete cellulases (endoglucanase, exoglucanase and β-glucosidase), xylanase and protease. Enzymatic ...
To gain insight into aberrant cytokine regulation in cystic fibrosis (CF), we compared the phenotypic manifestations of allergen challenge in gut-corrected CFTR-deficient mice with background-matched C57Bl6 (B6) mice. Aspergillus fumigatus (Af) antigen was used to mimic allergic bronchopulmonary aspergillosis, a peculiar hyper-IgE syndrome with a high prevalence in CF patients. CFTR-/-, C57BL/6 and FVB/NJ mice were sensitized with Af antigen by serial intraperitoneal injections. Control mice were mock sensitized with PBS. Challenges were performed by inhalation of Af antigen aerosol. After Af antigen challenge, histologic analysis showed goblet cell hyperplasia and lymphocytic infiltration in both strains. However, total serum IgE levels were markedly elevated in CF mice. Sensitized CF mice showed a five-fold greater IgE response to sensitization as compared with B6- and FVB-sensitized controls. Additional littermate controls to fully normalize for B6-FVB admixture in the strain background confirmed the
Invasive mycoses represent a major cause of morbidity and mortality in patients with malignancy or undergoing hematopoietic stem cell transplantation (HSCT). Recently it has been shown that the Toll-like receptor system could play an important role in the development of invasive aspergillosis in patients receiving chemotherapy or after HSCT, and there is evidence suggesting that the involvement of Toll-like receptors during Aspergillus fumigatus infection is influenced by the immunological status of the host. The upper and lower respiratory tracts represent the most frequent locations of A. fumigatus infection in all patient categories and age groups. Among echinocandins, caspofungin has been approved for salvage therapy in patients with invasive aspergillosis, since it has been demonstrated to be well-tolerated and with a 39% response in patients with refractory infections. However, in this study as in other salvage therapy trials, the need for salvage therapy was established in the presence of
An overview of Allergic Bronchopulmonary Aspergillosis (ABPA) symptoms, diagnosis, treatment & management written by experts in allergy, asthma & immunology.
Chaudhary N, Staab JF, Marr KA. PLoS One 2010;5:e9036.. Aspergillus allergens are described as proteins that are recognized in patients with hypersensitivity syndromes such as allergic bronchopulmonary aspergillosis and Aspergillus-induced asthma. However, findings from this study indicate that (at least) some of these proteins are not only allergens, but are also capable of inducing a T-helper 1 (Th1) cytokine response in volunteers without a history of suspected or proven fungal infection or reported allergy or atopy. This indicates that these "Asp f proteins" are able to induce both protective (Th1) and non-protective (Th2) inflammation. Depending on the hosts status, Aspergillus fumigatus is able to cause several diseases ranging from allergic (e.g. Aspergillus-induced asthma and allergic bronchopulmonary aspergillosis) to invasive (e.g. pulmonary aspergillosis and disseminated disease). A fumigatus conidia enter the body via the air and, if they are not cleared by phagocytic cells, spores ...
A collection of disease information resources and questions answered by our Genetic and Rare Diseases Information Specialists for Allergic bronchopulmonary aspergillosis
Overview: What every practitioner needs to know Are you sure your patient has allergic bronchopulmonary aspergillosis? What are the typical findings for this disease? Allergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity lung disease due to bronchial colonization by Aspergillus fumigatus that occurs in susceptible patients with asthma and cystic fibrosis (CF). ABPA affects approximately 1-2%…. ...
Overview: What every practitioner needs to know Are you sure your patient has allergic bronchopulmonary aspergillosis? What are the typical findings for this disease? Allergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity lung disease due to bronchial colonization by Aspergillus fumigatus that occurs in susceptible patients with asthma and cystic fibrosis (CF). ABPA affects approximately 1-2%…. ...
Minimal Diagnostic Criteria for ABPA-Central Bronchiectasis (ABPA-CB) Asthma Central bronchiectasis Immediate cutaneous reactivity to Aspergillus species or A. fumigatus Elevated total serum IgE (>417 kU/L) Elevated serum IgE-A.fumigatus and or IgG- A.fumigatus compared to sera from prick positive patients with asthma
Non-fumigatus Aspergillus species are the leading cause of Aspergillus infections in the tropical and subtropical regions of the world. In a prospective study between 2015 and 2016, a total of 150 bronchoalveolar (BAL) specimens was collected from patients suspected to pulmonary aspergillosis (PA) underlying immunodeficiencies in Mashhad, Northeastern Iran, located in the Middle East. All Aspergillus strains were phylogenetically identified at the species level by PCR-sequencing of partial β-tubulin gene. Overall, Aspergillus species were isolated from 20 specimens originating from 10 (50%) patients with cancer, 5 (25%) patients receiving corticosteroid therapy, 3 (15%) organ transplant recipients and 2 (10%) patients admitted to intensive care unit (ICU ...
Question - Have allergic aspergillosis. Would a flare up result in low blood oxygen level?. Ask a Doctor about diagnosis, treatment and medication for Allergic bronchopulmonary aspergillosis, Ask an OBGYN, Gynecologic Oncology
Many patients with cystic fibrosis (CF) cough up mucus or have throat cultures that grow a common fungus called Aspergillus. In patients with CF, aspergillus is not known to cause direct damage to the lungs, but some patients respond with an allergic reaction that causes them to wheeze, cough, or have difficulty breathing. This allergic reaction is called ABPA. Current treatment for ABPA includes high dose steroids and an anti-fungal medicine. Treatment with steroids may be problematic for some people due to its side effects on blood sugar levels and the bones. Steroids are medications that decrease inflammation, including prednisone, medrol, dexamethasone and others.. Ongoing research at UPMC on the study Mechanisms of Immune Tolerance in ABPA has studied people with CF and ABPA versus those patients with CF that just grow A. fumigatus (Af) in the sputum, but do not have ABPA. You may have participated in this study. This study has shown that people with CF with the fungus, Af, in their ...
By Jennifer Doucet & Anushka Jayasuriya - June 23, 2017. Microbes, including bacteria and fungi, have many tools to evade a host cells defenses and antimicrobial therapies. One tool is the creation of protective barriers called biofilms, made of proteins, DNA, and sugar polymers, which spread across surfaces and impede immune system responses and antimicrobial treatments. In fact, biofilms can make microbes up to 1000 times more resistant to treatment. Despite their critical role in resistance to antimicrobials, there are no currently licensed therapies that target mature biofilms.. The bacteria Pseudomonas aeruginosa and the fungus Aspergillus fumigatus both form sugar-rich biofilms in host tissues during infection. They cause serious infections worldwide, especially in immunocompromised patients or those with chronic disease. Additionally, co-colonization by both P. aeruginosa and A. fumigatus has been observed in patients with lung disease such as cystic fibrosis.. As recently published in ...
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Invasive aspergillosis is still a major fungal infection in immunocompromised patients. On the other hand, on time diagnosis of disease in the primary stages of infection is highly imperative for appropriate antifungal therapy and decreases the mortality rate in suspected groups (16). There are several approaches for detection and diagnosis of IA in clinical samples of patients. However, conventional methods, including direct examination and culture, are the gold standard to identify the causing agents, but there are some limitations, such as being time consuming, lack of proper sensitivity in early stages of the disease, as well as false negative results when the patients take the antifungal drugs (17).. Molecular diagnostic methods including real-time PCR as well as GM- EIA are more sensitive and specific and can approve an early IA diagnosis clinical outcome, when the other diagnostic methods are negative. Moreover, rapid results could be offered due to GM- EIA and real-time PCR assay ...
In recent years acquired azole resistance in Aspergillus fumigatus has been increasingly reported and a dominant mechanism of resistance (TR34/L98H) was found in clinical and environmental isolates. The aim of the present study was to investigate the prevalence of azole resistance in environmental A. fumigatus isolates collected in northern Italy. A. fumigatus grew from 29 of 47 soil samples analysed. Azole-resistant isolates were detected in 13% (6/47) of the soil samples and in 21% (6/29) of the soil samples containing A. fumigatus. High minimal inhibitory concentrations (MIC) of itraconazole (≥16 mg/L) and posaconazole (≥0.5 mg/L) were displayed by nine isolates from six different soil samples, namely apple orchard (1 sample), rose pot compost (2 samples), and cucurbit yields (3 samples). Seven isolates had a MIC=2 mg/L of voriconazole. Seven of nine itraconazole and posaconazole resistant isolates harboured the same TR34/L98H mutation of cyp51A. These findings, together with the occurrence of
Various saprotrophic microorganisms, especially filamentous fungi, can efficiently degrade lignocellulose that is one of the most abundant natural materials on earth. It consists of complex carbohydrates and aromatic polymers found in the plant cell wall and thus in plant debris. Aspergillus fumigatus Z5 was isolated from compost heaps and showed highly efficient plant biomass-degradation capability. The 29-million base-pair genome of Z5 was sequenced and 9540 protein-coding genes were predicted and annotated. Genome analysis revealed an impressive array of genes encoding cellulases, hemicellulases and pectinases involved in lignocellulosic biomass degradation. Transcriptional responses of A. fumigatus Z5 induced by sucrose, oat spelt xylan, Avicel PH-101 and rice straw were compared. There were 444, 1711 and 1386 significantly differently expressed genes in xylan, cellulose and rice straw, respectively, when compared to sucrose as a control condition. Combined analysis of the genomic and transcriptomic
Healthwise knowledgebase health facts. This records does no longer update the recommendation of a physician. Healthwise, incorporated, disclaims any guarantee or legal responsibility to your use of this data.. situations we treat nyu langone scientific middle. Nyu langone medical doctors and other professionals deal with heaps of situations, sicknesses, illnesses, and accidents. Learn extra.. Set definition of set by using the unfastened dictionary. Set (sĕt) n. Mythology variant of seth2. Set 1 (sĕt) v. Set, putting, sets v.Tr. 1. To put in a specified position or arrangement; region set a e-book on a table.. # diabetes causes yeast infection diabetic. ★ diabetes causes yeast infection ★ diabetic stuffed peppers the 3 step trick that reverses diabetes completely in as little as eleven days.[ diabetes reasons.. Aspergillus fumigatus and aspergillosis pubmed central. Aspergillus fumigatus is a saprophytic fungus that plays an critical position in recycling environmental carbon and nitrogen ...
Chitinase inhibitors have chemotherapeutic potential as fungicides, pesticides, and antiasthmatics. Argifin, a natural product cyclopentapeptide, competitively inhibits family 18 chitinases in the nanomolar to micromolar range and shows extensive substrate mimicry
Background Microsatellites (or short tandem repeats, STRs) are the genetic markers of choice for studying Aspergillus fumigatus molecular epidemiology due to its reproducibility and high discrimination power. However, the specificity of these markers must ...
In this study, we employed LSFM for the first time to simultaneously investigate the anatomical distribution of the pathogenic fungus A. fumigatus and define the immune cell populations in murine models of pulmonary fungal infection. LSFM proved superior to current gold standard techniques in providing quantitative data in the context of spatial anatomical information from analyses of large volumes of mouse lung tissue. Previously, the fungal burden (i.e., the degree of fungal growth) had been measured by CFU or by qPCR. Along these lines, quantification of the number of immune cells in whole lungs and phenotypic characterization of those cells were performed with flow cytometry. However, those methods require homogenization of the lungs and thus result in destruction of the anatomical context of lung tissues. In contrast, LSFM enabled us to quantify fungal burden in the intact tissue environment and to visualize differentiated A. fumigatus phenotypes and structures of hyphal growth invading the ...
Background Itraconazole (ITZ) is an alternative to steroids in the treatment of allergic bronchopulmonary aspergillosis (ABPA) but therapeutic drug monitoring (TDM) is warranted due to erratic absorption and early relapse.. Aim To correlate serum ITZ levels with clinical outcomes in ABPA.. Methods ITZ capsules 400mg/day for 6 months were administered as monotherapy to 30 consecutive patients in stage 4/5 of ABPA between Jan13 to Jun13. High-performance liquid chromatography was done on serum samples collected after 2-4 weeks to measure concentrations of ITZ and its active metabolite hydroxy-ITZ (OHITZ); ITZ levels of ,2mg/L were considered adequate. Total and Aspergillus species specific IgE, leucocyte count (TLC), eosinophil count (AEC) and precipitins were recorded before and thereafter. Any relapse was noted till 2 years.. Results Mean serum concentration of ITZ and OHITZ was 0.79±0.2 and 1.2±0.3mg/L respectively. Total IgE (9.9±1.4 vs 6.5±1.2×103IU/mL), specific IgE (42.98±3.9 vs ...
Disseminated aspergillosis has killed many patients who were being treated with immunosuppressive agents after organ transplantation. Few cases were recognized in patients before death, and those that were discovered were in terminally ill patients, and therapy was ineffective.. Four patients infected with Aspergillus fumigatus after renal homotransplantation are described. All infections were controlled successfully with amphotericin B. Immune suppression was continued and rejection crises treated with high doses of corticoids without extension of the fungal disease. Three of the patients required open lung biopsy to establish the diagnosis and stimulate accurate and prompt therapy. One patient was treated without biopsy ...
Allergic bronchopulmonary aspergillosis (ABPA) is a common but frequently misdiagnosed clinical condition. It is usually diagnosed in patients with a long standing history of asthma. Patients with ABPA can have diverse radiological manifestations. Very rarely, lung masses have been reported. We hereby report a case of ABPA in whom a large symptomatic lung mass was the presenting manifestation leading to consideration of lung cancer as a differential diagnosis. The establishment of ABPA as the underlying diagnosis led to conservative medical treatment which was followed by complete resolution of the mass like opacity. The present case highlights that ABPA should be considered as a differential diagnosis whenever encountering a patient with lung mass and history of asthma ...
In some patients a so-called "allergic bronchopulmonary Aspergillosis (ABPA)" delvelops. Hereby it is an allergic reaction of the patient to the colonization with the fungus. The involved patients are often apparent with a sudden increased cough, a narrowing of the bronchi and an obvious worsening of the lung function. The laboratory investigation shows an strong increase of the Immunoglobulin E and so-called precipitins which give a hint to the allergic reaction. In the thorax x-ray round shadows can be seen, which do often change the localization rapidly when doing a control ...
Covers manifold clinical manifestations, from allergic rhinitis to drug allergy and allergic bronchopulmonary aspergillosis Serves as a valuable tool
Gliotoxin: A fungal toxin produced by various species of Trichoderma, Gladiocladium fimbriatum, Aspergillus fumigatus, and Penicillium. It is used as an immunosuppressive agent.
Math o ganser syn cychwyn yn yr afu yw canser yr afu, a elwir hefyd yn ganser hepatig a chanser hepatig cynradd.[1] Mae canserau sydd wedi lledaeni ir afu o lefydd eraill yn y corff (metastasis yr afu) llawer mwy cyffredin na chyflyrau syn cychwyn yn yr afu.[2] Gall canser yr afu achosi symptomau megis ymdeimlad o boen neu lwmp gweledol islawr gawell asennau ar yr ochr dde, chwyddo yn yr abdomen, croen melyn, cleisio hawdd, colli pwysau a gwendid cyffredinol. Achosir y rhan fwyaf o gyflyrau canser yr afu gan sirosis o ganlyniad i hepatitis B, hepatitis C, neu alcohol.[3] Ymhlith yr achosion eraill y mae afflatocsin, afiechyd afu brasterog di-alcohol, a llyngyr yr afu. Y mathau mwyaf cyffredin or cyflwr yw carsinoma hepatogellol neu HCC (80% o achosion), a cholangiocarcinoma. Ceir rhai llai cyffredin yn ogystal, er enghraifft neoplasm systig mwsinog a neoplasm bustlaidd papilaidd anhydradwyol. Gellir cadarnhau diagnosis drwy brawf gwaed a delweddu meddygol a chanfod tystiolaeth bellach or ...
Hepatitis, Infection, Virus, Chronic Hepatitis, Chronic Hepatitis C, Hepatitis C, Hepatitis C Virus, Patients, Diseases, Hiv, Infections, Infectious Diseases, Toll-like Receptors, Association, Genetic Polymorphisms, Sample Size, Aspergillosis, Aspergillus, Aspergillus Fumigatus, Population
Affiliation:国立感染症研究所,真菌部,主任研究官, Research Field:Applied microbiology,Bacteriology (including Mycology),Infectious disease medicine, Keywords:プロテインキナーゼ,Aspergillus fumigatus,病原真菌,シグナル伝達,糸状菌,真菌症,アスペルギルス症,細胞周期,蛋白質リン酸化,ゲノム解析, # of Research Projects:4, # of Research Products:38, Ongoing Project:呼吸器糸状菌感染症のゲノム基盤構築による局所感染機構の解明と診断・治療法への応用
Aspergili (lat. Aspergillus) ali glavičaste plesni so rod ubikvitarnih (povsod razširjenih) plesni iz debla Ascomycota z značilnim konidijem.[2] Glive iz rodu Aspergillus so stalno prisotne v človekovem okolju. Najpogosteje gre za A. fumigatus, pogostejši so še A. flavus, A. terreus in A. niger. Spore gliv najdemo v gozdovih v kupih odpadlega listja, kompostih na vrtu, skladiščih žit, na gnijoči zelenjavi, v vlažnih kleteh stanovanjskih hiš, v iztrebkih ptic in tam, kjer potekajo kakršnakoli gradbena in vzdrževalna dela.[3] ...
The fungi Aspergillus flavus,[108] Aspergillus fumigatus,[108] Aspergillus niger,[108] Aspergillus parasiticus, Aspergillus ... tamarii, Aspergillus sulphureus, Aspergillus repens, Mucor hiemalis (not a human pathogen), Penicillium chrysogenum, ... Oven treatment killed conidia of A. fumigatus, A. flavus and A. niger, and did not degrade the active component of marijuana, ... Penicillium italicum and Rhizopus nigrans have been found in moldy cannabis.[107] Aspergillus mold species can infect the lungs ...
AF - Aspergillus fumigatus mutant C-2. *EC - Escherichia coli. *BT - Bacillus thuringiensis ...
lycopersici and Aspergillus fumigatus. Kanini, Grammatiki S.; Katsifas, Efstathios A.; Savvides, Alexandros L.; Karagouni, ...
Heddergott, C.; Calvo, A. M.; Latgé, J. P. (2017-02-02). "The Volatome of Aspergillus fumigatus". Eukaryotic Cell. 13 (8): 1014 ...
"Bioactive alkaloids from endophytic Aspergillus fumigatus". J Nat Prod. 72 (4): 753-5. doi:10.1021/np800700e. PMID 19256529. ... Hawas UW, El-Beih AA, El-Halawany AM (2012). "Bioactive anthraquinones from endophytic fungus Aspergillus versicolor isolated ... Fungi portal Bacillus isolates Biotechnology in pharmaceutical manufacturing Mycorrhiza Aspergillus oryzae, Saccharomyces ... two benzylazaphilones with an unprecedented carbon skeleton from the gorgonian-derived fungus Aspergillus sp". Bioorg Med Chem ...
Ge HM; Yu ZG; Zhang J; Wu JH; Tan RX (2009). "Bioactive alkaloids from endophytic Aspergillus fumigatus". J Nat Prod. 72 (4): ...
Rigbers, O.; Li, S.M. (2008). "Ergot alkaloid biosynthesis in Aspergillus fumigatus. Overproduction and biochemical ...
Aspergillus fumigatus, is a heterothallic fungus. It is one of the most common Aspergillus species to cause disease in humans ... Examples of heterothallism are included for Saccharomyces cerevisiae, Aspergillus fumigatus, Aspergillus flavus, Penicillium ... "Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus fumigatus". Nature. 457 (7228): 471-4. doi:10.1038 ... "Low genetic variation and no detectable population structure in aspergillus fumigatus compared to closely related Neosartorya ...
Ge HM, Yu ZG, Zhang J, Wu JH, Tan RX (2009). "Bioactive alkaloids from endophytic Aspergillus fumigatus". J Nat Prod. 72 (4): ... 14-Norpseurotin A is an alkaloid and a bio-active metabolite of Aspergillus, featuring an oxa-spiro-lactam core. Pseurotin A ... "Antiparasitic and anticancer constituents of the endophytic fungus Aspergillus sp. strain F1544". Nat Prod Commun. 7 (2): 165-8 ...
"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae". Nature. 438 (7071): 1105-15. ... Aspergillus nidulans Strain:FGSC A4, model organism (2005[20]). *Aspergillus niger Strain:ATCC 1015 (DOE Joint Genome institute ... Aspergillus oryzae Strain:RIB40, industrial use (2005[22]). *Aspergillus terreus NIH 2624, statin producer and pathogen (2005, ... "Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus". PLoS Genet. 4 (4): e1000046. doi:10.1371/journal. ...
Die fadenpilze, aspergillus flavus, niger u. fumigatus; eurotium repens (u. aspergillus glaucus) und ihre beziehungen zur ... otomycosis aspergillina, 1883 - The filamentous fungi, Aspergillus flavus, niger and fumigatus; Eurotium repens (Aspergillus ...
In Aspergillus fumigatus, a causative agent of aspergillosis, galactosaminogalactan is required for adherence to host tissue, ... Overlapping and distinct roles of Aspergillus fumigatus UDP-glucose 4-epimerases in galactose metabolism and the synthesis of ... 2014). A polysaccharide virulence factor from Aspergillus fumigatus elicits anti-inflammatory effects through induction of ... 2011). Galactosaminogalactan, a new immunosuppressive polysaccharide of Aspergillus fumigatus. In PLoS Pathog, pp. e1002372 ...
In Aspergillus fumigatus, the enzymes needed for gliotoxin biosynthesis are encoded in 13 genes within the gli gene cluster. ... The compound is produced by human pathogens such as Aspergillus fumigatus, and also by species of Trichoderma, and Penicillium ... Gliotoxin is hypothesized to be an important virulence factor in Aspergillus fumigatus. Experiments have demonstrated that ... "What do we know about the role of gliotoxin in the pathobiology of Aspergillus fumigatus?". Medical Mycology. 47: S97-103. doi: ...
Rarely, Aspergillus fumigatus and mucormycosis cause CST. The diagnosis of cavernous sinus thrombosis is made clinically, with ...
Opportunistic infection by molds[25] such as Penicillium marneffei and Aspergillus fumigatus is a common cause of illness and ... McCormick A, Loeffler J, Ebel F (November 2010). "Aspergillus fumigatus: contours of an opportunistic human pathogen". Cell. ... Light micrograph of the hyphae and spores of the human pathogen Aspergillus fumigatus ... an update on the pathogenesis of Aspergillus fumigatus infection". Br. J. Haematol. 150 (4): 406-17. doi:10.1111/j.1365- ...
For example, scientists detected that Aspergillus fumigatus var. fumigatus, a fungus which triggers allergic reactions in the ...
Opportunistic infection by molds such as Penicillium marneffei and Aspergillus fumigatus is a common cause of illness and death ... McCormick A, Loeffler J, Ebel F (November 2010). "Aspergillus fumigatus: contours of an opportunistic human pathogen". Cell. ... an update on the pathogenesis of Aspergillus fumigatus infection". Br. J. Haematol. 150 (4): 406-17. doi:10.1111/j.1365- ... Molds that are often found on meat and poultry include members of the genera Alternaria, Aspergillus, Botrytis, Cladosporium, ...
Beauvais, A; Latgé, JP (2001). "Membrane and cell wall targets in Aspergillus fumigatus". Drug Resist Updat. 4: 38-49. doi: ... Echinocandins are fungistatic against some molds (Aspergillus, but not Fusarium and Rhizopus), and modestly or minimally active ... They have fungistatic activity against Aspergillus species. and fungicidal activity against most Candida spp., including ...
... to the fungus Aspergillus (most commonly Aspergillus fumigatus). It occurs most often in patients with asthma or cystic ... Aspergillus spores are ubiquitous in soil and are commonly found in the sputum of healthy individuals. A. fumigatus is ... The first stage involves exposing the skin to Aspergillus fumigatus antigens; an immediate reaction is hallmark of ABPA. The ... Hohl, TM; Feldmesser, M (November 2007). "Aspergillus fumigatus: principles of pathogenesis and host defense". Eukaryotic cell ...
The fungus Aspergillus fumigatus effectively degrades plasticized PVC. Phanerochaete chrysosporium was grown on PVC in a ... Phanerochaete chrysosporium, Lentinus tigrinus, Aspergillus niger, and Aspergillus sydowii can effectively degrade PVC. ...
Matsuyama T; Tanaka K (1989). "On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose". Agric ... dianhydride from inulobiose by Aspergillus fumigatus". J. Biochem. Tokyo. 92 (4): 1325-8. PMID 6757245. Molecular and Cellular ...
"Aspergillus fumigatus: New Opportunities for Treatment of Multidrug-Resistant Fungal Disease". mBio. 8 (4): e01157-17. doi: ...
Aspergillus fumigatus and Aspergillus clavatus can cause allergic disease. Some Aspergillus species cause disease on grain ... The most common pathogenic species are Aspergillus fumigatus and Aspergillus flavus. Aspergillus flavus produces aflatoxin ... The spores of Aspergillus fumigatus are ubiquitous in the atmosphere. A. fumigatus is an opportunistic pathogen. It can cause ... O'Gorman CM, Fuller H, Dyer PS (2009). "Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus fumigatus ...
"Secondary metabolites from the endophytic fungi Penicillium polonicumand Aspergillus fumigatus". Journal of Asian Natural ...
"Protostadienol biosynthesis and metabolism in the pathogenic fungus Aspergillus fumigatus". Org. Lett. 11: 1241-1244. doi: ...
"Toll-like receptor 9-dependent immune activation by unmethylated CpG motifs in Aspergillus fumigatus DNA". Infect. Immun. 76 (5 ...
A. fumigatus strains and culture conditions: A. fumigatus strains used in this study are listed in Table 1. A. fumigatus ... 1994 Bidirectional gene transfer between Aspergillus fumigatus and Aspergillus nidulans. FEMS Microbiol. Lett. 122: 227-231. ... 2000 Aspergillus fumigatus, pp. 1-32 in Fungal Pathology, edited by Kronstad J. W.. Kluwer Academic, Dordrecht/Norwell, MA. ... 1999 Transformation systems of Aspergillus fumigatus. New tools to investigate fungal virulence. Contrib. Microbiol. 2: 149-166 ...
Aspergillus fumigatus, a ubiquitous human fungal pathogen, produces asexual spores (conidia), which are the main mode of ... MybA, a transcription factor involved in conidiation and conidial viability of the human pathogen Aspergillus fumigatus. ...
Aspergillus fumigatus. A. fumigatus causes more infections worldwide than any other mould. Four percent of all patients dying ... Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus.. Nierman WC, Pain A, Anderson MJ, ... Aspergillus fumigatus . This involved preparing a bacterial artificial chromosome (BAC) library, to fingerprint 3000 BAC clones ... The Sanger Institute was then funded to carry our a whole genome shotgun of A. fumigatus in collaboration with The Institute of ...
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... fumigatus has been associated with each and every type of health problems linked to environmental moulds: irritation and ... Aspergillus fumigatus Basics Laboratory Metabolites Adverse health reactions Specific settings Diagnostic Bibliography Basics A ... Aspergillus fumigatus is however the most important species in Aspergillus genus causing health problems. This species has been ... Health risks associated with exposure to Aspergillus fumigatus are well established. Most important, A. fumigatus is the main ...
Aspergillus fumigatus ATCC ® 28215™ Designation: AF4 Application: Biomedical Research and Development Material Emerging ... Aspergillus fumigatus Fresenius (ATCC® 28215™) Strain Designations: AF4 / Product Format: freeze-dried ...
Aspergillus fumigatus ATCC ® 1022™ Designation: NRRL 163 [118, CBS 133.61, IMI 16152, LSHB Ac71, NCTC 982, QM 1981, WB 163] ... Aspergillus fumigatus Fresenius (ATCC® 1022D-2™) Add to freeze-dried Total DNA: Approximately 2 µg in 1X Tris buffer. OD260/OD ... Aspergillus fumigatus Fresenius (ATCC® 1022™) Strain Designations: NRRL 163 [118, CBS 133.61, IMI 16152, LSHB Ac71, NCTC 982, ... Chemotypic and genotypic diversity in the ergot alkaloid pathway of Aspergillus fumigatus. Mycologia 104: 804-812, 2012. PubMed ...
Afut1, a retrotransposon-like element from Aspergillus fumigatus.. Neuveglise C1, Sarfati J, Latge JP, Paris S. ... A repeated DNA sequence used for epidemiological studies of the human opportunistic pathogen Aspergillus fumigatus has been ... This is the first report of a transposable element in A.fumigatus. Afut1 is a defective element: the putative coding domains ... Laboratoire des Aspergillus, Institut Pasteur, Paris, France.. Abstract. ...
Aspergillus fumigatus ATCC ® MYA-3626™ Designation: T33439 Application: Antifungal susceptibility testing Ref Ref Emerging ... Aspergillus fumigatus Fresenius (ATCC® MYA-3626™) Strain Designations: T33439 / Product Format: frozen ...
Aspergillus fumigatus ATCC ® MYA-4609™ Designation: CBS 101355 [AF 293] Application: Emerging infectious disease research ... Aspergillus fumigatus Fresenius (ATCC® MYA-4609D-2™) Add to dried Total DNA: Approximately 2 µg in 1X TE buffer. OD260/OD280: ... Aspergillus fumigatus Fresenius (ATCC® MYA-4609™) Strain Designations: CBS 101355 [AF 293] / Product Format: frozen ... Mistaken identity: Neosartorya pseudofischeri and its anamorph masquerading as Aspergillus fumigatus. J. Clin. Microbiol. 43: ...
Genomic DNA from Aspergillus fumigatus Strain 118 [ATCC ® 1022™] Application: ... Aspergillus fumigatus Fresenius (ATCC® 1022D-2™) Strain Designations: Genomic DNA from Aspergillus fumigatus Strain 118 [ATCC® ... Aspergillus fumigatus Fresenius ATCC® 1022D-2™ freeze-dried Total DNA: Approximately 2 µg in 1X Tris buffer. OD260/OD280: 1.6 ... Genomic DNA from Aspergillus fumigatus Strain 118 [ATCC® 1022™] Biosafety Level 1 Biosafety classification is based on U.S. ...
Aspergillus fumigatus ATCC ® MYA-3627™ Designation: FG 1432 Application: Antifungal susceptibility testing Ref Ref Emerging ... Aspergillus fumigatus Fresenius (ATCC® MYA-3627™) Strain Designations: FG 1432 / Product Format: frozen ...
... a mycotoxin from the fungus Aspergillus fumigatus. The researchers present their findings in the current issue of the ... The fungus Aspergillus fumigatus occurs virtually everywhere on Earth, as a dark grey, wrinkled cushion on damp walls or in ... Researchers find how Aspergillus fumigatus knocks out immune defenses. *Download PDF Copy ... This does not happen if the pathogen Aspergillus fumigatus is involved. As the Jena scientists were able to show, gliotoxin ...
HETEROCARYOSIS AND THE PARASEXUAL CYCLE IN ASPERGILLUS FUMIGATUS Message Subject (Your Name) has forwarded a page to you from ... HETEROCARYOSIS AND THE PARASEXUAL CYCLE IN ASPERGILLUS FUMIGATUS. Ȗistein Strømnæs and E. D. Garber ... HETEROCARYOSIS AND THE PARASEXUAL CYCLE IN ASPERGILLUS FUMIGATUS. Ȗistein Strømnæs and E. D. Garber ... HETEROCARYOSIS AND THE PARASEXUAL CYCLE IN ASPERGILLUS FUMIGATUS. Ȗistein Strømnæs and E. D. Garber ...
First Detection of TR34 L98H and TR46 Y121F T289A Cyp51 Mutations in Aspergillus fumigatus Isolates in the United States. J ... Azole Resistance in Aspergillus fumigatus: Can We Retain the Clinical Use of Mold-Active Antifungal Azoles? Clin Infect Dis 62: ... Multidrug-Resistant Aspergillus fumigatus Carrying Mutations Linked to Environmental Fungicide Exposure - Three States, 2010- ... The environmental mold Aspergillus fumigatus is the primary cause of invasive aspergillosis. In patients with high-risk ...
The genome sequences of three Aspergillus species-Aspergillus fumigatus, Aspergillus nidulans, and Aspergillus oryzae-were ... Aspergillus fumigatus is a fungus of the genus Aspergillus, and is one of the most common Aspergillus species to cause disease ... Conidia phialoconidia of Aspergillus fumigatus Colony in Petri dish Aspergillus fumigatus isolated from woodland soil Slide of ... Emergence of Azole Resistance in Aspergillus fumigatus and Spread of a Single Resistance Mechanism. at SciVee The Aspergillus ...
Homologous recombination - Aspergillus fumigatus [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Thermotolerance and virulence of Aspergillus fumigatus: role of the fungal nucleolus.. Bhabhra R1, Askew DS. ... As a thermotolerant fungus, Aspergillus fumigatus is capable of growth at temperatures that approach the upper limit for all ... fumigatus. Future work in this area will help determine how rapid growth is accomplished at elevated temperature and may offer ...
Characterization of a cell-wall acid phosphatase (PhoAp) in Aspergillus fumigatus.. Bernard M1, Mouyna I1, Dubreucq G1, ... In the filamentous fungus Aspergillus fumigatus, the vast majority of the cell-wall-associated proteins are secreted proteins ... Unité des Aspergillus, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France1.. 2. National and Kapodistrian ... fumigatus after cleavage of its anchor. It is also a phosphate-repressible acid phosphatase. The absence of PhoAp from a ...
A. Rivera, T. Hohl, and E. G. Pamer, "Immune responses to Aspergillus fumigatus infections," Biology of Blood and Marrow ... Elastase Activity in Aspergillus fumigatus Can Arise by Random, Spontaneous Mutations. Sergio Álvarez-Pérez,1 Jose L. Blanco,1 ... J.-P. Latgé, "The pathobiology of Aspergillus fumigatus," Trends in Microbiology, vol. 9, no. 8, pp. 382-389, 2001. View at ... J.-P. Latgé, "Aspergillus fumigatus and Aspergillosis," Clinical Microbiology Reviews, vol. 12, no. 2, pp. 310-350, 1999. View ...
Aspergillus niger (ASPNG), Aspergillus flavus (ASPFL), Aspergillus polyvalent mix (M1), Aspergillus fumagatus specific IgE (RM3 ... Immunodiffusion (Precipitin Aspergillus Fumigatus #6 IgG). Related Tests. Allergic Bronchopulmonary Aspergillosis: ... Immunoglobulin E level (IGE), Aspergillus fumagatus #1 (ASPF1), ...
Aspergillus fumigatus strains resistant to all triazole antifungal medications are emerging in the United States in patients ... Aspergillus fumigatus strains resistant to all triazole antifungal medications are emerging in the United States in patients ... First detection of TR34 L98H and TR46 Y121F T289A Cyp51 mutations in Aspergillus fumigatus isolates in the United States. J ... First detection of TR34 L98H and TR46 Y121F T289A Cyp51 mutations in Aspergillus fumigatus isolates in the United States. J ...
Specific surveys of the air for Aspergillus fumigatus were carried out in rural and urban situations over a 2-year period. ... Sources and Incidence of Airborne Aspergillus Fumigatus (Fres) Clin Allergy. 1976 May;6(3):209-17. doi: 10.1111/j.1365- ... Specific surveys of the air for Aspergillus fumigatus were carried out in rural and urban situations over a 2-year period. ... This availability of decaying plant debris with high water content fulfils the growth requirements of Aspergillus fumigatus and ...
Aspergillus fumigatus / chemistry, metabolism*. Carbohydrate Sequence. Coenzyme A / metabolism. Ethanolamines / metabolism. ... Aspergillus fumigatus, a filamentous fungus, produces GPI-anchored molecules, some of them being essential in the construction ... Unité des Aspergillus, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France. [email protected] ... This latter series of GPI intermediates identified in the A. fumigatus cell-free system indicates that GPI biosynthesis in this ...
  • In summary, MybA protein is a new key regulator of conidiogenesis and conidial maturation and survival, and plays a crucial role in propagation and virulence of A. fumigatus. (pasteur.fr)
  • The effect of lipoprotein-associated phospholipase A2 deficiency on pulmonary allergic responses in Aspergillus fumigatus sensitized mice. (nih.gov)
  • Several pulmonary diseases have been associated with A. fumigatus , such as allergic bronchopulmonary aspergillosis, severe asthma with fungal sensitization, rhinitis, sinusitis, and hypersensitivity pneumonitis. (hindawi.com)
  • Although A. fumigatus is a source of 23 listed allergens [ 17 ] and is an opportunistic pathogen, A. fumigatus -induced pulmonary diseases may or may not involve elevated serum IgE or fungal colonization [ 18 - 20 ]. (hindawi.com)
  • As our understanding of the host pathogen interaction in the genesis of an A. fumigatus -induced pulmonary disease is emerging, models that mimic natural human exposure are critical. (hindawi.com)
  • In neutropenic mice infected with wild-type A. fumigatus, increased pulmonary expression of E-selectin, cytokine-induced neutrophil chemoattractant (KC), and TNF-alpha occurred only when neutropenia had resolved. (nih.gov)
  • In nonneutropenic mice immunosuppressed with corticosteroids, A. fumigatus stimulated earlier pulmonary expression of E-selectin, VCAM-1, and KC, while expression of intercellular adhesion molecule 1 and TNF-alpha was suppressed. (nih.gov)
  • Aspergillus nodule Chronic cavitary pulmonary aspergillosis (CCPA) where cavities are present in the lungs, but not necessarily with a fungal ball (aspergilloma). (wikipedia.org)
  • C. M. Tang, J. Cohen, and D. W. Holden, "An Aspergillus fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity," Molecular Microbiology , vol. 6, no. 12, pp. 1663-1671, 1992. (hindawi.com)
  • Desta forma, o objetivo do presente estudo foi a expressão, caracterização e validação como alvo quimioterapêutico do gene Afpmr1 de A. fumigatus. (usp.br)
  • Paralelamente, para a RNAi, um fragmento do gene Afpmr1 apresentando baixa identidade com outros genes de cálcio ATPases de diferentes espécies foi clonado em vetor de expressão em A. fumigatus (pALB1). (usp.br)
  • Estes resultados mostram que o gene Afpmr1 pode ser expresso funcionalmente em sistemas heterólogos e seu silenciamento, em A. fumigatus, influencia processos celulares que podem estar relacionados à manutenção da estrutura e composição da parede celular, além de desencadear alterações na fagocitose e killing de macrófagos. (usp.br)
  • In this way, the aim of the present work was the expression, characterization e validation, as chemotherapeutic target, of the A. fumigatus Afpmr1 gene. (usp.br)
  • At the same time, a fragment of the Afpmr1 gene, showing low identity values for another calcium ATPase genes, was cloned in an A. fumigatus expression vector (pALB1) for RNAi. (usp.br)
  • These results show that the Afpmr1 gene can be functionally expressed in eukaryotic heterologous systems and its silencing, in A. fumigatus, alters cellular processes that can be related with the maintenance of the cell wall structure and composition, as well as promote alterations in the macrophages phagocytosis and killing. (usp.br)
  • To provide a global and accurate understanding of A. fumigatus in vitro BF growth, we utilized microarray, RNA-sequencing, and proteomic analysis to compare the global gene and protein expression profiles of A. fumigatus grown under BF and PL conditions. (nih.gov)
  • Cloning and characterization of chsD, a chitin synthase-like gene of Aspergillus fumigatus. (nih.gov)
  • A chitin synthase-like gene (chsD) was isolated from an Aspergillus fumigatus genomic DNA library. (nih.gov)
  • Here it is shown that global gene expression of A. fumigatus and A. niger is markedly different upon contact with A549 cells. (springer.com)
  • These annotations include Gene Ontology (GO) terms, phenotype data, gene names and descriptions and they are crucial for interpreting both small- and large-scale data and for aiding in the design of new experiments that further Aspergillus research. (nih.gov)
  • This set of 266 manually curated secondary metabolite gene clusters will facilitate the investigation of novel Aspergillus secondary metabolites. (nih.gov)
  • The abr1 gene (for "aspergillus brown 1") encodes a putative protein possessing two signatures of multicopper oxidases. (asm.org)
  • Involvement of the six genes in conidial pigmentation was confirmed by the altered conidial color phenotypes that resulted from disruption of each gene in A. fumigatus . (asm.org)
  • First, essentiality of the A. fumigatus AfFKS1 gene, encoding the catalytic subunit of the β-(1,3)-glucan synthase complex, was assessed by inactivating one allele of AfFKS1 in a diploid strain of A. fumigatus obtained using adequate selectable markers in spore color and nitrate utilization pathways and by performing haploidization under conditions that select for the occurrence of the disrupted allele. (genetics.org)
  • Genomic analysis of these heterozygous diploids led in particular to the identification of an essential A. fumigatus gene encoding an SMC-like protein resembling one in Schizosacccharomyces pombe involved in chromosome condensation and cohesion. (genetics.org)
  • Using 63 soil cores, we cultured for azole-resistant A. fumigatus and characterized isolates by their cyp51A gene type, short tandem repeat genotype, and mating type. (cdc.gov)
  • It is based on the A. fumigatus pyrG gene, encoding orotidine 5'-monophosphate decarboxylase, which was cloned and sequenced. (nih.gov)
  • This provides a tool to target defined genetic constructs at a specific locus in the A. fumigatus genome in order to study gene regulation and function. (nih.gov)
  • also called TNF-stimulated gene 6, TSG-6) and selected microorganisms, including Aspergillus fumigatus and Pseudomonas aeruginosa. (wikipedia.org)
  • Serial determinations of IgE reactivity to individual purified Aspergillus antigens, especially Asp f3, demonstrates that increases in IgE reactivity may provide improved distinction between stages of flares and remission compared with changes in IgE reactivity to a crude Aspergillus extract. (nih.gov)
  • Time course of antibody response to recombinant Aspergillus fumigatus antigens in cystic fibrosis with and without ABPA. (nih.gov)
  • Six monoclonal antibodies produced against Aspergillus fumigatus were studied for their cross-reactivity against other fungal antigens from related and unrelated organisms. (nih.gov)
  • Our first attempts in this direction and particularly in the enhancement of a protective immunity in the immunocomprornised host, have lead us to the study of the two major antigens of A. fumigatus, a dipeptidylpeptidase V and a catalase. (aspergillus.org.uk)
  • The reaction of IgE with Aspergillus antigens results in mast cell degranulation with bronchoconstriction and increased capillary permeability. (wikipedia.org)
  • The ImmunoCAP technology has replaced this time consuming, labor-intensive method with their automated CAP assays and FEIA (Fluorescence enzyme immunoassay) that can detect IgG antibodies against Aspergillus fumigatus (Farmer's lung or for ABPA) or avian antigens (Bird Fancier's Lung). (wikipedia.org)
  • We determined follow-up levels of specific serum IgE to the recombinant Aspergillus fumigatus (A. fumigatus) allergens rAsp f 1, 3, 4 and 6 in patients suffering from cystic fibrosis (CF) with and without allergic bronchopulmonary aspergillosis (ABPA). (nih.gov)
  • Allergic bronchopulmonary aspergillosis (ABPA) is a condition characterised by an exaggerated response of the immune system (a hypersensitivity response) to the fungus Aspergillus (most commonly Aspergillus fumigatus). (wikipedia.org)
  • Aspergillus fumigatus is the main causative agent of aspergillosis. (springer.com)
  • In Aspergillus fumigatus, a causative agent of aspergillosis, galactosaminogalactan is required for adherence to host tissue, to mask PAMPs like β-1,3-glucans and to mediate virulence in several animal models. (wikipedia.org)
  • For one of the major fungal pathogens, the environmental mold Aspergillus fumigatus , an interesting and relevant morphotype beyond the conidial and hyphal stages has gained increasing attention: the multicellular biofilm. (frontiersin.org)
  • The opportunistic pathogenic mold Aspergillus fumigatus is an increasing cause of morbidity and mortality in immunocompromised and in part immunocompetent patients. (nih.gov)
  • Aspergillus fumigatus is a saprobic mold that thrives on decaying plant material. (cdc.gov)
  • Aspergillus fumigatus is a saprophytic mold whose natural habitat is decaying plant material ( 1 ). (cdc.gov)
  • Aspergillus terreus, also known as Aspergillus terrestris, is a fungus (mold) found worldwide in soil. (wikipedia.org)
  • Functional genomic profiling of Aspergillus fumigatus biofilm reveals enhanced production of the mycotoxin gliotoxin. (nih.gov)
  • To identify other GPI-anchored proteins putatively involved in cell wall biogenesis, a proteomic analysis has been undertaken in A. fumigatus and the protein data were matched with the yeast genomic data. (nih.gov)
  • A. fumigatus grown on certain building materials can produce genotoxic and cytotoxic mycotoxins, such as gliotoxin. (wikipedia.org)
  • Studies with DeltagliP and DeltastuA null mutants of A. fumigatus indicated that the extent of endothelial cell stimulation was not influenced by gliotoxin or other StuA-dependent factors synthesized by A. fumigatus. (nih.gov)
  • A. fumigatus stimulation of cell E-selectin expression requires direct endothelial cell contact but not endocytosis of the organism and is not influenced by gliotoxin or other stuA -dependent secondary metabolites. (nih.gov)
  • without signs of antibody- or cell-mediated adaptive immune response or symptoms attributable to A. fumigatus inhalation ( Park and Mehrad, 2009 ). (frontiersin.org)
  • The importance of the host immune status has been underlined by the lack of identified specific virulence attributes in A. fumigatus ( 1 , 3 - 6 ). (rupress.org)
  • 2013). Aspergillus Galactosaminogalactan Mediates Adherence to Host Constituents and Conceals Hyphal β-Glucan from the Immune System. (wikipedia.org)
  • The prevalence of serum IgE antibodies to Dermatophagoides pteronyssinus (house-dust mite), Aspergillus fumigatus, and bovine beta-lactoglobulin was significantly greater in cases of sudden infancy death (S.I.D.) than in a control group of infants of the same age range. (bmj.com)
  • Tests used to diagnose an aspergilloma may include: Chest X-ray Chest CT Sputum culture Bronchoscopy or bronchoscopy with lavage (BAL) Serum precipitins for aspergillus (blood test to detect antibodies to aspergillus) Almost all aspergillomas are caused by Aspergillus fumigatus. (wikipedia.org)