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Aspartic Acid ProteasesAspartic AcidPepstatinsAspartic Acid EndopeptidasesProtease InhibitorsEndopeptidasesNorleucinePeptide HydrolasesRhizopusMucorAmino Acid SequenceCathepsin DAspergillus oryzaeMolecular Sequence DataPepsin AHydrogen-Ion ConcentrationCathepsin EChymosinMitosporic FungiHIV ProteaseAmino AcidsCathepsinsAspergillus nigerSaccharomycopsisNitrophenolsPepsinogensSerine EndopeptidasesBinding SitesEpoxy CompoundsDiazonium CompoundsCladosporiumAspergillusAzo CompoundsXylarialesBase SequenceMutagenesis, Site-DirectedKineticsMolecular WeightPectobacterium carotovorumEncyclopedias as Topic2S Albumins, PlantCastor BeanAntigens, T-IndependentYolk SacPara-Aortic BodiesImmunoglobulin MCaspase 3RabbitsB-LymphocytesFusariumTrichothecenesMycotoxinsTriticumZearalenonePlant DiseasesCereals
Aspartic Acid Proteases
A subclass of peptide hydrolases that depend on an ASPARTIC ACID residue for their activity.
Aspartic Acid
One of the non-essential amino acids commonly occurring in the L-form. It is found in animals and plants, especially in sugar cane and sugar beets. It may be a neurotransmitter.
Pepstatins
N-acylated oligopeptides isolated from culture filtrates of Actinomycetes, which act specifically to inhibit acid proteases such as pepsin and renin.
Aspartic Acid Endopeptidases
A sub-subclass of endopeptidases that depend on an ASPARTIC ACID residue for their activity.
Protease Inhibitors
Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).
Endopeptidases
A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.
Norleucine
An unnatural amino acid that is used experimentally to study protein structure and function. It is structurally similar to METHIONINE, however it does not contain SULFUR.
Peptide Hydrolases
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
Rhizopus
A genus of zygomycetous fungi of the family Mucoraceae, order MUCORALES, a common saprophyte and facultative parasite of mature fruits and vegetables. It may cause cerebral mycoses in diabetes and cutaneous infection in severely burned patients.
Mucor
A genus of zygomycetous fungi of the family Mucoraceae, order Mucorales. It is primarily saprophytic, but may cause MUCORMYCOSIS in man from spores germinating in the lungs.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Cathepsin D
An intracellular proteinase found in a variety of tissue. It has specificity similar to but narrower than that of pepsin A. The enzyme is involved in catabolism of cartilage and connective tissue. EC 3.4.23.5. (Formerly EC 3.4.4.23).
Aspergillus oryzae
An imperfect fungus present on most agricultural seeds and often responsible for the spoilage of seeds in bulk storage. It is also used in the production of fermented food or drink, especially in Japan.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Pepsin A
Formed from pig pepsinogen by cleavage of one peptide bond. The enzyme is a single polypeptide chain and is inhibited by methyl 2-diaazoacetamidohexanoate. It cleaves peptides preferentially at the carbonyl linkages of phenylalanine or leucine and acts as the principal digestive enzyme of gastric juice.
Hydrogen-Ion Concentration
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Cathepsin E
An aspartic endopeptidase that is similar in structure to CATHEPSIN D. It is found primarily in the cells of the immune system where it may play a role in processing of CELL SURFACE ANTIGENS.
Chymosin
The predominant milk-clotting enzyme from the true stomach or abomasum of the suckling calf. It is secreted as an inactive precursor called prorennin and converted in the acid environment of the stomach to the active enzyme. EC 3.4.23.4.
Mitosporic Fungi
A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.
HIV Protease
Enzyme of the human immunodeficiency virus that is required for post-translational cleavage of gag and gag-pol precursor polyproteins into functional products needed for viral assembly. HIV protease is an aspartic protease encoded by the amino terminus of the pol gene.
Amino Acids
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Cathepsins
A group of lysosomal proteinases or endopeptidases found in aqueous extracts of a variety of animal tissues. They function optimally within an acidic pH range. The cathepsins occur as a variety of enzyme subtypes including SERINE PROTEASES; ASPARTIC PROTEINASES; and CYSTEINE PROTEASES.
Aspergillus niger
An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.
Saccharomycopsis
Yeast-like ascomycetous fungi of the family Saccharomycopsidaceae, order SACCHAROMYCETALES, isolated from the stomach of rabbits and some other animals.
Nitrophenols
Pepsinogens
Proenzymes secreted by chief cells, mucous neck cells, and pyloric gland cells, which are converted into pepsin in the presence of gastric acid or pepsin itself. (Dorland, 28th ed) In humans there are 2 related pepsinogen systems: PEPSINOGEN A (formerly pepsinogen I or pepsinogen) and PEPSINOGEN C (formerly pepsinogen II or progastricsin). Pepsinogen B is the name of a pepsinogen from pigs.
Serine Endopeptidases
Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.
Binding Sites
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Epoxy Compounds
Organic compounds that include a cyclic ether with three ring atoms in their structure. They are commonly used as precursors for POLYMERS such as EPOXY RESINS.
Diazonium Compounds
Cladosporium
A mitosporic Loculoascomycetes fungal genus including some economically important plant parasites. Teleomorphs include Mycosphaerella and Venturia.
Aspergillus
A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.
Azo Compounds
Xylariales
An order of ascomycetous FUNGI which includes many economically important plant parasites as well as saprophytes.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Mutagenesis, Site-Directed
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Kinetics
The rate dynamics in chemical or physical systems.
Molecular Weight
The sum of the weight of all the atoms in a molecule.
Pectobacterium carotovorum
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that causes rotting, particularly of storage tissues, of a wide variety of plants and causes a vascular disease in CARROTS; and POTATO plants.
Encyclopedias as Topic
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
2S Albumins, Plant
A major class of water-soluble seed storage proteins. Many proteins from this class are major PLANT ALLERGENS.
Castor Bean
Common name for Ricinus communis, a species in the family EUPHORBIACEAE. It is the source of CASTOR OIL.
Antigens, T-Independent
Antigens which may directly stimulate B lymphocytes without the cooperation of T lymphocytes.
Yolk Sac
The first of four extra-embryonic membranes to form during EMBRYOGENESIS. In REPTILES and BIRDS, it arises from endoderm and mesoderm to incorporate the EGG YOLK into the DIGESTIVE TRACT for nourishing the embryo. In placental MAMMALS, its nutritional function is vestigial; however, it is the source of INTESTINAL MUCOSA; BLOOD CELLS; and GERM CELLS. It is sometimes called the vitelline sac, which should not be confused with the VITELLINE MEMBRANE of the egg.
Para-Aortic Bodies
Small masses of chromaffin cells found near the SYMPATHETIC GANGLIA along the ABDOMINAL AORTA, beginning cranial to the superior mesenteric artery (MESENTERIC ARTERY, SUPERIOR) or renal arteries and extending to the level of the aortic bifurcation or just beyond. They are also called the organs of Zuckerkandl and sometimes called aortic bodies (not to be confused with AORTIC BODIES in the THORAX). The para-aortic bodies are the dominant source of CATECHOLAMINES in the FETUS and normally regress after BIRTH.
Immunoglobulin M
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
Caspase 3
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Rabbits
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
B-Lymphocytes
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Fusarium
A mitosporic Hypocreales fungal genus, various species of which are important parasitic pathogens of plants and a variety of vertebrates. Teleomorphs include GIBBERELLA.
Trichothecenes
Usually 12,13-epoxytrichothecenes, produced by Fusaria, Stachybotrys, Trichoderma and other fungi, and some higher plants. They may contaminate food or feed grains, induce emesis and hemorrhage in lungs and brain, and damage bone marrow due to protein and DNA synthesis inhibition.
Mycotoxins
Toxic compounds produced by FUNGI.
Triticum
A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.
Zearalenone
(S-(E))-3,4,5,6,8,10-Hexahydro-14,16-dihydroxy-3-methyl-1H-2-benzoxacyclotetradecin-1,7(8H)-dione. One of a group of compounds known under the general designation of resorcylic acid lactones. Cis, trans, dextro and levo forms have been isolated from the fungus Gibberella zeae (formerly Fusarium graminearum). They have estrogenic activity, cause toxicity in livestock as feed contaminant, and have been used as anabolic or estrogen substitutes.
Plant Diseases
Diseases of plants.
Cereals
Seeds from grasses (POACEAE) which are important in the diet.

Cloning of the SAP6 gene of Metschnikowia reukaufii and its heterologous expression and characterization in Escherichia coli. (1/51)

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Criteria for the differentiation between young and old Onchocerca volvulus filariae. (2/51)

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Recognition of fungal protease activities induces cellular activation and eosinophil-derived neurotoxin release in human eosinophils. (3/51)

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Identification of novel aspartic proteases from Strongyloides ratti and characterisation of their evolutionary relationships, stage-specific expression and molecular structure. (4/51)

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A novel bifunctional peptidic aspartic protease inhibitor inhibits chitinase A from Serratia marcescens: Kinetic analysis of inhibition and binding affinity. (5/51)

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Feline immunodeficiency virus (FIV) as a model for study of lentivirus infections: parallels with HIV. (6/51)

FIV is a significant pathogen in the cat and is, in addition, the smallest available natural model for the study of lentivirus infections. Although divergent at the amino acid level, the cat lentivirus has an abundance of structural and pathophysiological commonalities with HIV and thus serves well as a model for development of intervention strategies relevant to infection in both cats and man. The following review highlights both the strengths and shortcomings of the FIV/cat model, particular as regards development of antiviral drugs.  (+info)

Neutralizing antibodies to the hookworm hemoglobinase Na-APR-1: implications for a multivalent vaccine against hookworm infection and schistosomiasis. (7/51)

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Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity. (8/51)

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Aspartic acid proteases in yeast - DTU Research DatabaseAspartic acid proteases in yeast - DTU Research Database
The production of the presumed GPI anchored, intracellular enzyme, Yapsin1, was investigated. The intra- and the extracellular protein production was followed as a function of growth in order to define the optimal conditions of the production of the enzyme, both in terms of secretion and in terms of amounts. The secreted protein was sequenced ...
https://orbit.dtu.dk/en/projects/aspartic-acid-proteases-in-yeast
OSAP 2018 Annual Conference Program - OSAPOSAP 2018 Annual Conference Program - OSAP
Substitutions within this program are gladly accepted. A substitution of your full registration is permitted prior to the conference by submitting a written request to [email protected] Please note there will be a $25 substitution fee for any change made to registrations. Onsite transfers are not permitted. Only one substitution is permitted per original registrant. The individual submitting the substitution request is responsible for all financial obligations (any balance due) associated with that substitution before the change can be made. Badge sharing, splitting, and reprints are strictly prohibited.. OSAP offers registrants contact information to facilitate networking after the course. By registering, you give OSAP permission to include your name and contact name in the list. If you do not wish to be included in the list, email your exclusion request to [email protected] by May 15, 2018.. OSAP takes photos during the course. By registering, you give OSAP permission to use any images taken at ...
http://osap.site-ym.com/?page=2018AnnConfProgram
Aspartic protease - WikipediaAspartic protease - Wikipedia
Aspartic proteases are a catalytic type of protease enzymes that use an activated water molecule bound to one or more aspartate residues for catalysis of their peptide substrates. In general, they have two highly conserved aspartates in the active site and are optimally active at acidic pH. Nearly all known aspartyl proteases are inhibited by pepstatin. Aspartic endopeptidases EC 3.4.23. of vertebrate, fungal and retroviral origin have been characterised.[1] More recently, aspartic endopeptidases associated with the processing of bacterial type 4 prepilin[2] and archaean preflagellin have been described.[3][4] Eukaryotic aspartic proteases include pepsins, cathepsins, and renins. They have a two-domain structure, arising from ancestral duplication. Retroviral and retrotransposon proteases (retroviral aspartyl proteases) are much smaller and appear to be homologous to a single domain of the eukaryotic aspartyl proteases. Each domain contributes a catalytic Asp residue, with an extended active ...
https://en.wikipedia.org/wiki/Aspartate_protease
EP 1030911 A1 20000830 - HUMAN ASPARTIC PROTEASESEP 1030911 A1 20000830 - HUMAN ASPARTIC PROTEASES
293476355 - EP 1030911 A1 2000-08-30 - HUMAN ASPARTIC PROTEASES - [origin: WO0004137A1] The invention provides human aspartic proteases (NHAP) and polynucleotides which identify and encode NHAP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of NHAP.[origin: WO0004137A1] The invention provides human aspartic proteases (NHAP) and polynucleotides which identify and encode NHAP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of NHAP.
https://data.epo.org/gpi/EP1030911A1-HUMAN-ASPARTIC-PROTEASES
SMART: Pfam domain A1 PropeptideSMART: Pfam domain A1 Propeptide
This entry represents the N-terminal domain of the aspartic peptidases. Aspartic peptidase, also known as aspartyl proteases ([intenz:3.4.23.-]) are a widely distributed family of proteolytic enzymes [ (PUBMED:6795036) (PUBMED:2194475) (PUBMED:1851433) ] known to exist in vertebrates, fungi, plants, retroviruses and some plant viruses. Aspartate proteases of eukaryotes are monomeric enzymes which consist of two domains. Each domain contains an active site centred on a catalytic aspartyl residue. The two domains most probably evolved from the duplication of an ancestral gene encoding a primordial domain. Currently known eukaryotic aspartyl proteases are: ...
http://smart.embl.de/smart/do_annotation.pl?DOMAIN=Pfam:A1_Propeptide&START=22&END=50&E_VALUE=7e-14&TYPE=PFAM&BLAST=LHRVPLRRHQSLRKKLRAQGQLSEFWRSH
US 6613764 B1 - Aspartic Protease Inhibitors - The Lens - Free & Open Patent and Scholarly SearchUS 6613764 B1 - Aspartic Protease Inhibitors - The Lens - Free & Open Patent and Scholarly Search
The Lens serves almost all the patents and scholarly work in the world as a free, open and secure digital public good, with user privacy a paramount focus.
https://www.lens.org/lens/patent/021-691-933-637-183/collections
Aspartic peptidase, N-terminal (IPR012848) | InterPro | EMBL-EBIAspartic peptidase, N-terminal (IPR012848) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
http://www.ebi.ac.uk/interpro/entry/IPR012848
Identification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD) | Journal of...Identification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD) | Journal of...
Dendritic cells (DC) are known to present exogenous protein Ag effectively to T cells. In this study we sought to identify the proteases that DC employ during antigen processing. The murine epidermal-derived DC line Xs52, when pulsed with PPD, optimally activated the PPD-reactive Th1 clone LNC.2F1 as well as the Th2 clone LNC.4k1, and this activation was completely blocked by chloroquine pretreatment. These results validate the capacity of XS52 DC to digest PPD into immunogenic peptides inducing antigen specific T cell immune responses. XS52 DC, as well as splenic DC and DCs derived from bone marrow degraded standard substrates for cathepsins B, C, D/E, H, J, and L, tryptase, and chymases, indicating that DC express a variety of protease activities. Treatment of XS52 DC with pepstatin A, an inhibitor of aspartic acid proteases, completely abrogated their capacity to present native PPD, but not trypsin-digested PPD fragments to Th1 and Th2 cell clones. Pepstatin A also inhibited cathepsin D/E activity
https://jibtherapies.biomedcentral.com/articles/10.1186/1476-8518-2-8/metrics
Napsin A Detection: ELISAs and AntibodiesNapsin A Detection: ELISAs and Antibodies
Novel Aspartic Proteinase of the PepSIN Family (Napsin A, or NAPSA) belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. It is also known as aspartyl protease 4 (ASP4), KAP, Kdap, napsin-1, NAP1, NAPA, and SNAPA. Two closely related proteins are known, Napsin A and Napsin B. Napsin A is a single-chain, 38-kDa protein. It is expressed at high levels in human lung and kidney, and at lower levels in spleen. Napsin A expression has been detected in type II pneumocytes and in lung adenocarcinomas.. ...
http://www.clontech.com/US/Products/Cell_Biology_and_Epigenetics/Cancer_and_Inflammation/Napsin_A?sitex=10020:22372:US&PEBCL1=brr6T3ZJr2ZxcK27YO2uTZVB07&PEBCL1_pses=ZG3D11BE890CE399DA771AAB4F7C369DC940A52BE7B2A00156C6DE1E4B7B028095B2E08E9CAE5D0F58FB36DC9D1639C305D7B6251025805666
Protease LASSO Screening Libraries / Protease Libraries / Targeted and Focused Screening Libraries | Life ChemicalsProtease LASSO Screening Libraries / Protease Libraries / Targeted and Focused Screening Libraries | Life Chemicals
Inhibition of HIV protease (HIVPR) or HIV reverse transcriptase (HIVRT) are two approaches to block viral replication. HIVPR is an aspartic acid protease that cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Inhibition of its activity disrupts HIVs ability to replicate and infect additional cells. HIVRT is an RNA-dependent DNA polymerase that catalyzes the conversion/transcription of single-stranded RNA into DNA. Normal transcription involves the synthesis of RNA from DNA; hence, reverse transcription is the reverse of this. Discovery of small molecule inhibitors of these targets is facilitated by the use of ligand and receptor based screening using two different targeted libraries available from Life Chemicals available:. ...
https://lifechemicals.com/screening-libraries/targeted-and-focused-screening-libraries/protease-libraries/protease-lasso-screening-libraries
IST Research ExplorerIST Research Explorer
Zhao Y, Wu L, Fu Q, Wang D, Li J, Yao B, Yu S, Jiang L, Qian J, Zhou X, Han L, Zhao S, Ma C, Zhang Y, Luo C, Dong Q, Li S, Zhang L, Jiang X, Li Y, Luo H, Li K, Yang J, Luo Q, Li L, Peng S, Huang H, Zuo Z, Liu C, Wang L, Li C, He X, Friml J, Du Y. 2021. INDITTO2 transposon conveys auxin-mediated DRO1 transcription for rice drought avoidance. Plant, Cell & Environment ...
https://research-explorer.app.ist.ac.at/record?limit=100&start=40&style=ista
IST Research ExplorerIST Research Explorer
Zhao Y, Wu L, Fu Q, Wang D, Li J, Yao B, Yu S, Jiang L, Qian J, Zhou X, Han L, Zhao S, Ma C, Zhang Y, Luo C, Dong Q, Li S, Zhang L, Jiang X, Li Y, Luo H, Li K, Yang J, Luo Q, Li L, Peng S, Huang H, Zuo Z, Liu C, Wang L, Li C, He X, Friml J, Du Y. 2021. INDITTO2 transposon conveys auxin-mediated DRO1 transcription for rice drought avoidance. Plant, Cell & Environment ...
https://research-explorer.app.ist.ac.at/groups/IST?cql=type%3C%3Eresearch_data&limit=50&start=0&style=ista
Design and Synthesis of Inhibitors Targeting the Aspartic Proteases HIV-1 PR and BACE-1Design and Synthesis of Inhibitors Targeting the Aspartic Proteases HIV-1 PR and BACE-1
This thesis describes the synthesis of molecules designed for inhibition of two aspartic proteases, viral HIV-1 PR and human BACE-1. It also reports on the structure activity relationships of the targeted enzyme inhibitors.. It is estimated that currently 33 million people are infected with HIV, the causative agent of AIDS. The virus targets T-lymphocytes and macrophages of the human immune system. The HIV-1 PR plays an important role in the viral replication, and by inhibiting the enzyme the disease progression can be slowed down or even halted.. Herein is reported the design and synthesis of a series of HIV-1 PR inhibitors with novel P2 substituents of which several inhibit the enzyme in the nanomolar range. The aim of the second work was to further develop the inhibitors by the introduction of fluorine. Several attempts were performed to fluorinate different P2-substituents.. Alzheimers disease (AD) is neurodegenerative, progressive and fatal disorder of the brain. It is associated with ...
http://www.diva-portal.org/smash/record.jsf?dswid=-6003&pid=diva2%3A235142&c=10&searchType=SIMPLE&language=en&query=&af=%5B%5D&aq=%5B%5B%7B%22organisationId%22%3A%22631%22%7D%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all
Pledge - OSAPPledge - OSAP
OSAP thanks its Super Sponsors for their support in 2017. Sponsorship does not imply endorsement by OSAP of a companys products or services. ...
http://www.osap.org/page/Pledge/Pledge.htm
Protease - GyDB orgProtease - GyDB org
LTR retroelement-like proteases (PRs, also named here as APs) are proteolytic enzymes that play a key role in the maturation process during which several peptides involved in the life cycle of the retroelement are scissed by this enzyme. LTR retroelement PRs belong to clan AA of aspartic peptidases (Rawlings et al. 2008); they dimerize in their active form and may be encoded as a part of the pol polyprotein, alone or as a part of the gag polyprotein, or in frame with a dUTPase (see dUTPase section). It is well known that the structural PR homodomain is founded in a core ~90-150 residues long wherein the catalytic DTG motif (Pearl and Blundell 1984) is the most prominent feature along with a glycine at the C-terminal end preceded by two hydrophobic residues (Pearl and Taylor 1987). At the primary structure level the most conserved part (core) of all clan peptidases may be divided in six amino acidic patterns constituting a template we have called DTG/ILG. We introduce this template in a ...
https://gydb.org/index.php?title=Protease&oldid=100
The Toxoplasma Blog: Aspartic Proteases and a Novel, Dynamic and Cell-Cycle-Dependent Protease Localization in ToxoThe Toxoplasma Blog: Aspartic Proteases and a Novel, Dynamic and Cell-Cycle-Dependent Protease Localization in Toxo
Aspartic proteases are important virulence factors in pathogens like HIV, Candida albicans or Plasmodium falciparum. We report here the identification of seven putative aspartic proteases, TgASP1 to TgASP7, in the apicomplexan parasite Toxoplasma gondii. Bioinformatic and phylogenetic analysis of the TgASPs and other aspartic proteases from related Apicomplexa suggests the existence of five distinct groups of aspartic proteases with different evolutionary lineages. The members of each group share predicted biological features that validate the phylogeny. TgASP1 is expressed in tachyzoites, the rapidly dividing asexual stage of T.gondii. We present the proteolytic maturation and subcellular localization of this protease through the cell cycle. TgASP1 shows a novel punctate localization associated with the secretory system in non-dividing cells, and relocalizes dramatically and unambiguously to the nascent inner membrane complex of daughter cells at replication, before coalescing again at the end ...
http://toxoplasmaparasite.blogspot.com/2007/06/aspartic-proteases-and-novel-dynamic.html
SDV Dental Practices - OSAPSDV Dental Practices - OSAP
The information above is a resource prepared by the Organization for Safety, Asepsis and Prevention (OSAP) with the assistance and expertise of its members. OSAP is a nonprofit, independent organization providing information and education on infection control and prevention and patient and provider safety to dental care settings worldwide. This resource is an overview with links to more detailed information. Additional relevant information is available on CDC, OSHA, EPA, OSAP and other websites. Content provided is current at time of publication. OSAP assumes no liability for actions taken based on information herein.. ...
http://osap.site-ym.com/page/SDVDentalPractices
Protease Inhibitors | Thermo Fisher ScientificProtease Inhibitors | Thermo Fisher Scientific
Protease inhibitor cocktails and tablets target serine, cysteine, and aspartic acid proteases, and aminopeptidases. Metalloproteases are inhibited by the addition of EDTA, which is available in a separate vial in the liquid format, but included in the tablet format.. Thermo Scientific Halt liquid cocktails are available in 100 μL single-use format or 1, 5 and 10 mL pack sizes; the Thermo Scientific Pierce tablets come in two sizes for 10 or 50 mL volumes, to accommodate different volume and pricing needs. These tablets are formulated to dissolve quickly into a clear solution, and are fully compatible with all Pierce protein assays.. ...
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation/protease-phosphatase-inhibition/protease-inhibitors.html
eNews - November 2013 - The Australian Wine Research InstituteeNews - November 2013 - The Australian Wine Research Institute
Recent AWRI staff publications1559 Muhlack, R. Its time to power up. WBM (August): 39-41; 2013.1560 Dry, P. Ask the AWRI: Vines: Is an oldie necessarily a goodie? Aust. N.Z. Grapegrower Winemaker (596): p. 57; 2013.1561 Varela, C., Chambers, P., Johnson, D. Trials turn up new strategies for softening the kick in wine. Aust. N.Z. Grapegrower Winemaker (596): 70-73; 2013.1562 Carew, A.L., Smith, P., Close, D.C., Curtin, C., Dambergs, R.G. Yeast effects on Pinot noir wine phenolics, color, and tannin composition. J. Agric. Food Chem. 61 (41): 9892-9898; 2013.1563 van Sluyter, S.C., Warnock, N.I., Schmidt, S., Anderson, P., van Kan, J.A.L., Bacic, a., Waters, E.J. Aspartic acid protease from Botrytis cinerea removes haze-formation proteins during white winemaking. J. Agric. Food Chem. 61 (40): 9705-9711; 2013.1564 Pojer, E., Mattivi, F., Johnson, D., Stockley, C.S. The case for anthocyanin consumption to promote human health: A review. Comp. Rev. Food Sci. Food Safety 12 (5): 483-508; 2013.1565 ...
http://www.awri.com.au/information_services/enews/2013/11/08/nov-2013/
NewPage4 - OSAPNewPage4 - OSAP
Foundation General Fund » Donate Online. The OSAP Foundation is an educational foundation that supports education, research, service and policy development to promote safety and the control of infectious diseases in dental care settings worldwide. Contributions to the Foundation, a tax-exempt organization under Section 501(c)(3) of the Internal Revenue Code, are deductible for computing income and estate taxes. The tax identification number for the OSAP Foundation is 52-1963109. ...
http://www.osap.org/page/NewPage4/NewPage4.htm
Cascade synthesis with (triphenylphosphoranylidene)ethenone as a versatile reagent for fast synthesis of heterocycles and...Cascade synthesis with (triphenylphosphoranylidene)ethenone as a versatile reagent for fast synthesis of heterocycles and...
Today, the demand for speed in drug discovery is constantly increasing, particularly in the iterative processes of hit validation and expansion and lead optimization. Irradiation with microwaves (MWs) has been applied in the area of organic synthesis to accelerate chemical reactions and to facilitate the generation of new chemical entities since 1986. In the work presented in this thesis, the use of MW-mediated heating has been expanded to address three fields of drug discovery, namely hit expansion, chemical library generation and genomics.. In the first project, potential inhibitors of malaria aspartic proteases were designed and synthesized, partly by MW-assisted organic chemistry, and evaluated with regard to their inhibitory efficacy on five malaria aspartic proteases and their selectivity over two human aspartic proteases. The synthetic work included the development of fast and convenient methods of MW-assisted formation of thiazolidines and epoxy esters. Some of the resulting structures ...
http://uu.diva-portal.org/smash/record.jsf?pid=diva2:211425
Patent US20110098321 - Salts of methyl 2-((r)-(3-chlorophenyl)((r)-1-((s)-2-(methylamino)-3-((r ... - Google PatentsPatent US20110098321 - Salts of methyl 2-((r)-(3-chlorophenyl)((r)-1-((s)-2-(methylamino)-3-((r ... - Google Patents
Mucic acid salts of a compound represented by the following structural formula: are disclosed. In particular, single crystalline mucic acid salts of the compound represented by structural formula (I) are characterized by a variety of properties and physical measurements. Methods of producing the mucic acid salts, using the salts to antagonize one or more aspartic proteases, and methods of treating a number of aspartic protease mediated disorders using the salts are described herein.
https://www.google.co.uk/patents/US20110098321
Osap | Dental Product ShopperOsap | Dental Product Shopper
Dental Product Shopper delivers practical, unbiased product information. Our peer to peer product reviews help you select the best products for your practice.
http://www.dentalproductshopper.com/manufacturer-home/osap
Gentaur Molecular :Nacala \ D Aspartic Acid \ 03501-34Gentaur Molecular :Nacala \ D Aspartic Acid \ 03501-34
Gentaur molecular products has all kinds of products like :search , Nacala \ D_ Aspartic Acid \ 03501-34 for more molecular products just contact us
http://www.antibody-antibodies.com/product_det.php?id=1085504&supplier=search&name=D_%20Aspartic%20Acid
SAP130抗体|Abcam中国|Anti-SAP130抗体(ab114978)SAP130抗体|Abcam中国|Anti-SAP130抗体(ab114978)
SAP130兔多克隆抗体(ab114978)可与人样本反应并经IP实验严格验证。中国75%以上现货，所有产品均提供质保服务，可通过电话、电邮或微信获得本地专属技术支持。
http://www.abcam.cn/sap130-antibody-ab114978.html
Plasmodium falciparum - encyclopedia article - CitizendiumPlasmodium falciparum - encyclopedia article - Citizendium
A lot of research is being done on Plasmodium falciparum in order to get rid of the disease. Researchers are hoping to discover new drugs and vaccines that could treat the disease or remove it entirely from the world. Researchers have been trying to find a new drug that would terminate the function of an important enzyme used by P. falciparum. They would like to discover a drug that can inhibit plasmepsins I and II, Plm I and Plm II. In P. falciparum, Plm I is the first to cleave the hemoglobin. Plm II also cleaves but it mainly takes action against denatured hemoglobin. Plm I and Plm II is said to be homologous to aspartic acid proteases, such as Cathepsin D (Cat D) in mammals. It would good if researchers can find an antimalarial drug that can stop Plm I from breaking down hemoglobin so that the P. falciparum parasite can die. Pepstatin A inhibits aspartic protease and prevents hemoglobin degradation. So this might be a successful drug. Researchers are also in the process of creating ...
http://en.citizendium.org/wiki/Plasmodium_falciparum
An n →π* Interaction in the Bound Substrate of Aspartic Proteases Replicates the Oxyanion HoleAn n →π* Interaction in the Bound Substrate of Aspartic Proteases Replicates the Oxyanion Hole
Aspartic proteases regulate many biological processes and are prominent targets for therapeutic intervention. Structural studies have captured intermediates along the reaction pathway, including the Michaelis complex and tetrahedral intermediate. Using a Ramachandran analysis of these structures, we discovered that residues occupying the P1 and P1′ positions (which flank the scissile peptide bond) adopt the dihedral angle of an inverse γ-turn and polyproline type-II helix, respectively. Computational analyses reveal that the polyproline type-II helix engenders an n→π∗ interaction in which the oxygen of the scissile peptide bond is the donor. This interaction stabilizes the negative charge that develops in the tetrahedral intermediate, much like the oxyanion hole of serine proteases. The inverse γ-turn serves to twist the scissile peptide bond, vacating the carbonyl π∗ orbital and facilitating its hydration. These previously unappreciated interactions entail a form of ...
https://dspace.mit.edu/handle/1721.1/125571
Microbiology Society Journals | Spirochaeta americana sp. nov., a new haloalkaliphilic, obligately anaerobic spirochaete...Microbiology Society Journals | Spirochaeta americana sp. nov., a new haloalkaliphilic, obligately anaerobic spirochaete...
A novel, obligately anaerobic, mesophilic, haloalkaliphilic spirochaete, strain ASpG1T, was isolated from sediments of the alkaline, hypersaline Mono Lake in California, USA. Cells of the Gram-negative strain were motile and spirochaete-shaped with sizes of 0·2-0·22×8-18 μm. Growth of the strain was observed between 10 and 44 °C (optimum 37 °C), in 2-12 % (w/v) NaCl (optimum 3 % NaCl) and between pH 8 and 10·5 (optimum pH 9·5). The novel strain was strictly alkaliphilic, required high concentrations of carbonates in the medium and was capable of utilizing d-glucose, fructose, maltose, sucrose, starch and d-mannitol. End products of glucose fermentation were H2, acetate, ethanol and formate. Strain ASpG1T was resistant to kanamycin and rifampicin, but sensitive to gentamicin, tetracycline and chloramphenicol. The G+C content of its DNA was 58·5 mol%. DNA-DNA hybridization analysis of strain ASpG1T with its most closely related species, Spirochaeta alkalica Z-7491T, revealed a hybridization value
http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02535-0
RCSB PDB - 1APT: CRYSTALLOGRAPHIC ANALYSIS OF A PEPSTATIN ANALOGUE BINDING TO THE ASPARTYL PROTEINASE PENICILLOPEPSIN...RCSB PDB - 1APT: CRYSTALLOGRAPHIC ANALYSIS OF A PEPSTATIN ANALOGUE BINDING TO THE ASPARTYL PROTEINASE PENICILLOPEPSIN...
1APT: Crystallographic Analysis of a Pepstatin Analogue Binding to the Aspartyl Proteinase Penicillopepsin at 1.8 Angstroms Resolution
http://www.rcsb.org/pdb/explore/materialsAndMethods.do?structureId=1APT
Structure Cluster - 1APT: CRYSTALLOGRAPHIC ANALYSIS OF A PEPSTATIN ANALOGUE BINDING TO THE ASPARTYL PROTEINASE...Structure Cluster - 1APT: CRYSTALLOGRAPHIC ANALYSIS OF A PEPSTATIN ANALOGUE BINDING TO THE ASPARTYL PROTEINASE...
1APT: Crystallographic Analysis of a Pepstatin Analogue Binding to the Aspartyl Proteinase Penicillopepsin at 1.8 Angstroms Resolution
http://www.rcsb.org/pdb/explore/structureCluster.do?structureId=1APT
Exploring the uses of pepstatin A | AG Scientific BlogExploring the uses of pepstatin A | AG Scientific Blog
Pepstatin A is of microbial origin and is an N-acyl-pentapeptide, more accurately: isovaleryl-L-valyl-L-valyl-statyl-L-alanyl-statine. Pepstatin A was found to be a potent competitive inhibitor of most aspartic proteases but a weak inhibitor of renin.
https://agscientific.com/blog/2012/03/exploring-the-uses-of-pepstatin-a/
Aspartic Acid Market Size, Share & Trends Report, 2022Aspartic Acid Market Size, Share & Trends Report, 2022
Global aspartic acid demand was 35.6 kilo tons in 2012. Increasing biodegradable products demand owing to depleting fossil fuel reserves is expected to remain a key driving factor for global aspartic acid market for the next seven years
https://www.grandviewresearch.com/industry-analysis/aspartic-acid-market
Penis Enlargement Products: (GNC) What Is D Aspartic Acid Used For GolanWinesPenis Enlargement Products: (GNC) What Is D Aspartic Acid Used For GolanWines
What Is D Aspartic Acid Used For how to take sizegenix, What Is D Aspartic Acid Used For adderall xr 20 mg cost => GolanWines 22 06 2019
https://www.golanwines.co.il/sexual-enhancers/what-is-d-aspartic-acid-used-for.html
Application and appreciation of chemical sand fixing agent-poly (aspartic acid) and its composites - GeoScience.netApplication and appreciation of chemical sand fixing agent-poly (aspartic acid) and its composites - GeoScience.net
Yang, J.; Cao, H.; Wang, F.; Tan, T., 2007: Application and appreciation of chemical sand fixing agent-poly (aspartic acid) and its composites
https://geoscience.net/research/030/147/030147692.php
Aspartic Acid Market Size Forecast to Reach $121.9 Million by 2025 - iCrowdNewswireAspartic Acid Market Size Forecast to Reach $121.9 Million by 2025 - iCrowdNewswire
Aspartic Acid Market size is forecast to reach $121.9 Million by 2025, after growing at a CAGR of 6.5% during 2020-2025. Increased demand
https://icrowdnewswire.com/2020/12/07/aspartic-acid-market-size-forecast-to-reach-121-9-million-by-2025/
Plasmepsin - WikipediaPlasmepsin - Wikipedia
Plasmepsins are a class of at least 10 enzymes (EC 3.4.23.38 and EC 3.4.23.39) produced by the Plasmodium falciparum parasite. There are ten different isoforms of these proteins and ten genes coding them respectively in Plasmodium (Plm I, II, III, IV, V, VI, VII, IX, X and HAP). It has been suggested that the plasmpesin family is smaller in other human Plasmodium species. Expression of Plm I, II, IV, V, IX, X and HAP occurs in the erythrocytic cycle, and expression of Plm VI, VII, VIII, occurs in the exoerythrocytic cycle. Through their haemoglobin-degrading activity, they are an important cause of symptoms in malaria sufferers. Consequently, this family of enzymes is a potential target for antimalarial drugs. Plasmepsins are aspartic acid proteases, meaning their active site contains two aspartic acid residues. These two aspartic acid residue act respectively as proton donor and proton acceptor, catalysing the hydrolysis of peptide bond in proteins. There are four types of plasmepsins, closely ...
https://en.wikipedia.org/wiki/Plasmepsin
A fourth secreted aspartyl proteinase gene (SAP4) and a CARE2 repetitive element are located upstream of the SAP1 gene in...A fourth secreted aspartyl proteinase gene (SAP4) and a CARE2 repetitive element are located upstream of the SAP1 gene in...
Candida albicans secreted aspartyl proteinases (Sap), products of the SAP genes, which are presumed to act as virulence factors. In the C. albicans strain WO-1, the ability to secrete Sap1 is regulated with switch phenotype, another putative virulence factor. KpnI restriction fragment length polymorphisms differentiate between several distinct SAP1 alleles in laboratory and clinical strains. Both SAP1 alleles from strain WO-1 along with their 5- and 3-flanking regions were cloned and sequenced, as were both alleles from another strain, SS. The 5-flanking regions were remarkably similar in all four of the sequenced alleles over approximately 1,500 nucleotides. S1 analysis revealed that both alleles of WO-1 are transcribed. Characterization of the one allele from strain WO-1 identified a 284-nucleotide insertion flanked by 8-bp direct repeats that shows homology to the CARE2 repetitive element and that is not present in the other alleles. Characterization of the SAP1 alleles also identified a ...
https://jb.asm.org/content/176/6/1702
Beta Secretase 1 (Aspartyl Protease 2 or Beta Site Amyloid Precursor Protein Cleaving Enzyme 1 or Memapsin 2 or Membrane...Beta Secretase 1 (Aspartyl Protease 2 or Beta Site Amyloid Precursor Protein Cleaving Enzyme 1 or Memapsin 2 or Membrane...
Beta Secretase 1 (Aspartyl Protease 2 or Beta Site Amyloid Precursor Protein Cleaving Enzyme 1 or Memapsin 2 or Membrane Associated Aspartic Protease 2 or BACE1 or EC 3.4.23.46) - Pipeline Review, H2 2018
https://www.researchandmarkets.com/reports/4658163/beta-secretase-1-aspartyl-protease-2-or-beta
Bioline International Official Site (site up-dated regularly)Bioline International Official Site (site up-dated regularly)
is one of the three main causative agents of human schistosomiasis, a major health problem with a vast socio-economic impact. Recent advances in the proteomic analysis of schistosomes have revealed that peptidases are the main virulence factors involved in the pathogenesis of this disease. In this context, evolutionary studies can be applied to identify peptidase families that have been expanded in genomes over time in response to different selection pressures. Using a phylogenomic approach, we searched for expanded endopeptidase families in the S. mansoni predicted proteome with the aim of contributing to the knowledge of such enzymes as potential therapeutic targets. We found three endopeptidase families that comprise leishmanolysins (metallopeptidase M8 family), cercarial elastases (serine peptidase S1 family) and cathepsin D proteins (aspartic peptidase A1 family). Our results suggest that the Schistosoma members of these families originated from successive gene duplication events in the ...
http://www.bioline.org.br/abstract?id=oc11175&lang=en
1-Aspartic Acid | definition of 1-Aspartic Acid by Medical dictionary1-Aspartic Acid | definition of 1-Aspartic Acid by Medical dictionary
Looking for online definition of 1-Aspartic Acid in the Medical Dictionary? 1-Aspartic Acid explanation free. What is 1-Aspartic Acid? Meaning of 1-Aspartic Acid medical term. What does 1-Aspartic Acid mean?
https://medical-dictionary.thefreedictionary.com/1-Aspartic+Acid
Natural products inhibiting Candida albicans secreted aspartic proteases from Tovomita krukovii.Natural products inhibiting Candida albicans secreted aspartic proteases from Tovomita krukovii.
Assay-guided fractionation of the ethanol extract of Tovomita krukovii resulted in the identification of four new xanthones (1 - 4) and ten known compounds (5 - 14). The structures of compounds 1 - 14 were determined by spectral data to be 3,5-dihydr
http://www.biomedsearch.com/nih/Natural-products-inhibiting-Candida-albicans/11842327.html
News DetailsNews Details
In conjunction with this month-long emphasis on the critical role of infection control in dentistry, OSAP is launching a major collaborative program called Safest Dental Visit™. This new educational program encourages a greater commitment to infection control and safety and helps dental teams educate and train staff, promote a safe and professional environment, increase patient confidence and loyalty, attract new patients and enhance the image of the practice.. As an OSAP Super Sponsor, Patterson Dental serves as a strategic partner for messaging in OSAPs infection control initiatives. Recently Patterson Dental was named a co-recipient of the 2015 Dr. Milton Schaefer Award for leadership and support in advancing OSAPs mission to be the leading advocate for the safe and infection-free delivery of oral healthcare.. ...
https://www.pattersondental.com/News/NewsDetails/45
REN Protein, renin - Creative BioMartREN Protein, renin - Creative BioMart
Prorenin is a glycosylated aspartic protease that consists of 2?homologouslobes and is the precursor of renin. Renin activates the renin-angiotensinsystem by cleaving angiotensinogen, produced by the liver, to yield angiotensinI, which is further converted into angiotensin II by ACE, theangiotensin-converting enzyme primarily within the capillaries of the lungs. Ithas been reported that the levels of circulating prorenin (but not renin) areincreased in diabetic subjects.
https://www.creativebiomart.net/symbolsearch_REN.htm
2:19 pm2:19 pm
Related Articles. Structure-Based Design of Inhibitors of the Aspartic Protease Endothiapepsin by Exploiting Dynamic Combinatorial Chemistry.. Angew Chem Int Ed Engl. 2014 Feb 14 ...
http://atumcg.cmb.med.rug.nl/?p=25451
D Aspartic Acid Testosterone EnhancementD Aspartic Acid Testosterone Enhancement
D Aspartic Acid Testosterone Enhancement is the best way to use an over the counter non steroidal product to increase your testosterone naturally!
http://mindandmuscle.net/articles/d-aspartic-acid-testosterone-enhancement/
July 2009 | The Homestead HobbyistJuly 2009 | The Homestead Hobbyist
No, I am not one. But I did apply today for the course here in DC. Interviews are in December, and the course is in February and March. 2.5 hours every Tuesday and Thursday night. Geez! The 50 hours of volunteering afterward is much easier to swallow--I volunteer anywhere from five to 15 hours in any given week anyway (an average of about six or seven, however--15 is peak time, which has been in June and December for me here), three or four of which is at a Master Gardener-approved volunteering place already (the Youth Garden). For a lot of people, I think, the 50 hours of volunteering is more difficult than the book learning and the test, but for me? Thatll be a snap ...
http://www.indoorgarden-er.com/2009_07_01_archive.html
Financial Aid | Nipissing UniversityFinancial Aid | Nipissing University
OSAP provides funding for postsecondary students with financial need to help support their educational costs. OSAP also provides supplementary funding for students with disabilities to help cover additional disability-related educational expenses (see below).. We, Student Accessibility Services (SAS) at Nipissing University, recommend that you complete the OSAP application process to see if you may be eligible for disability-related funding to help cover additional disability-related educational costs that you may have such as tutoring, or note taking support, or assistive technology. To qualify, you must demonstrate at least $1 of financial need.. When you apply for OSAP online, you can self-identify as a student with a permanent disability by checking off a box on the form. You will need to provide documentation of your disability to Student Accessibility Services, and we will verify your status as a student with a permanent disability to the Financial Aid Office and to OSAP.. Some of the ...
https://acquiadev.nipissingu.ca/departments/student-development-and-services/accessibility-services/financial-aid
Patent US7115652 - Aspartyl protease inhibitors - Google PatentenPatent US7115652 - Aspartyl protease inhibitors - Google Patenten
The present invention provides compounds having the formula: wherein R1, R′, R2, R3, R3′, R4, X1, X2 and X3 are as defined herein, and pharmaceutical compositions thereof. The present invention also provides methods of inhibiting proteases, more specifically aspartyl proteases. In certain embodiments, compounds inhibit BACE (β-site APP-cleaving enzyme), and thus are useful in the treatment or prevention of a disease characterized by β-amyloid deposits in the brain (including, but not limited to, Alzheimers Disease). The present invention also provides methods for preparing compounds of the invention.
http://www.google.nl/patents/US7115652
Plasmepsins -ORCAPlasmepsins -ORCA
This chapter elaborates the structural chemistry and the biological aspects of plasmepsins. Plasmepsin I is capable of cleaving native human hemoglobin and acid-denatured globin, with a pH optimum around 5. Plasmepsin I, II, IV and HAP are made as 51 kDa precursors that are cleaved to 37 kDa mature forms. There are two disulfide bonds predicted. The second domain contains an active-site Asp-Ser-Gly instead of the usual aspartic protease Asp-Thr-Gly. HAP is an exception to this. A molecular model of plasmepsin I has been constructed, based on the crystal structure of the highly homologous plasmepsin II. The modeled structure bears general features of mammalian and fungal aspartic proteases, and among mammalian orthologs shares greatest structural similarity with cathepsin D. Pepstatin is bound in the active site with enough conformational difference from mammalian homologs to give hope for development of a selective inhibitor. Native plasmepsin I, II and HAP can be prepared in nanogram to low ...
https://orca.cf.ac.uk/64839/
Alexander Wlodawer, Ph.D. | Center for Cancer Research - National Cancer InstituteAlexander Wlodawer, Ph.D. | Center for Cancer Research - National Cancer Institute
Dr. Wlodawer is a structural biologist investigating the relationship between protein structure and function, mainly by high-resolution X-ray diffraction. Some of his areas of interest include elucidating structural features of macromolecules that could explain their importance to understanding cancer and AIDS. Examples include proteases, viral glycoproteins and enzymes, and
https://ccr.cancer.gov/macromolecular-crystallography-laboratory/alexander-wlodawer
RNAseq revealed the important gene pathways controlling adaptive mechanisms under waterlogged stress in maize | Scientific...RNAseq revealed the important gene pathways controlling adaptive mechanisms under waterlogged stress in maize | Scientific...
Waterlogging causes yield penalty in maize-growing countries of subtropical regions. Transcriptome analysis of the roots of a tolerant inbred HKI1105 using RNA sequencing revealed 21,364 differentially expressed genes (DEGs) under waterlogged stress condition. These 21,364 DEGs are known to regulate important pathways including energy-production, programmed cell death (PCD), aerenchyma formation, and ethylene responsiveness. High up-regulation of invertase (49-fold) and hexokinase (36-fold) in roots explained the ATP requirement in waterlogging condition. Also, high up-regulation of expansins (42-fold), plant aspartic protease A3 (19-fold), polygalacturonases (16-fold), respiratory burst oxidase homolog (12-fold), and hydrolases (11-fold) explained the PCD of root cortical cells followed by the formation of aerenchyma tissue during waterlogging stress. We hypothesized that the oxygen transfer in waterlogged roots is promoted by a cross-talk of fermentative, metabolic, and glycolytic pathways that
https://www.nature.com/articles/s41598-017-10561-1?error=cookies_not_supported&code=40f535e4-b719-41a0-844c-c43a5d3f6d37
10. Gastrointestinal Nematode Vaccines10. Gastrointestinal Nematode Vaccines
Hookworm filariform Ac-APR -1 is a cathepsin D aspartic protease from A. caninum which initiates digestive cascade ⇒ If you can block this activity it should
https://www.immunopaedia.org.za/teaching-learning-tools/other-courses/immunology-in-the-tropics/gastrointestinal-nematode-vaccines/
Amount of Aspartic acid, D-AA, Asp, or D, α-amino acid in Burdock root, raw naturalAmount of Aspartic acid, D-AA, Asp, or D, α-amino acid in Burdock root, raw natural
How much of Aspartic acid, D-AA, Asp, or D, α-amino acid is present in Burdock root, raw natural in details, quantity how high or low Aspartic acid, D-AA, Asp, or D, α-amino acid nutrient content it has.
http://www.traditionaloven.com/foods/specific-nutrient/veggies/burdock-root-raw/aspartic-acid-d-aa-asp.html
ChemIDplus - 190195-65-4 - Aspartic acid, N-((1S)-1,2-dicarboxyethyl)-3-hydroxy-, sodium salt (1:4) - Searchable synonyms,...ChemIDplus - 190195-65-4 - Aspartic acid, N-((1S)-1,2-dicarboxyethyl)-3-hydroxy-, sodium salt (1:4) - Searchable synonyms,...
190195-65-4 - Aspartic acid, N-((1S)-1,2-dicarboxyethyl)-3-hydroxy-, sodium salt (1:4) - Searchable synonyms, formulas, resource links, and other chemical information.
https://chem.nlm.nih.gov/chemidplus/rn/190195-65-4
education Archives - Off the Cuspeducation Archives - Off the Cusp
Are you up-to-date on the latest guidelines and standards from CDC, OSAP, OSHA and your state government? Do you stay current with industry news and continuing education? Develop more confidence in your infection control management by increasing focus on training, documentation and ongoing education. Follow […]. ...
https://www.offthecusp.com/tag/education/
Amyloid-beta precursor protein secretaseAmyloid-beta precursor protein secretase
BACE is a transmembrane protein with an extracellular aspartic acid protease domain. γ-secretase is actually a protein complex ... Presenilin is believed to harbor the protease domain and represents an important example of an uncommon type of protease that ...
https://en.wikipedia.org/wiki/Amyloid-beta_precursor_protein_secretase
NeurodegenerationNeurodegeneration
Caspases (cysteine-aspartic acid proteases) cleave at very specific amino acid residues. There are two types of caspases: ... Plaques are made up of small peptides, typically 39-43 amino acids in length, called beta-amyloid (also written as A-beta or Aβ ... CAG codes for the amino acid glutamine. A repeat of CAG results in a polyglutamine (polyQ) tract. Diseases associated with such ... A current therapeutic target for the treatment of Alzheimer's disease is the protease β-secretase[non-primary source needed], ...
https://en.wikipedia.org/wiki/Neurodegeneration
Prepilin peptidasePrepilin peptidase
LaPointe CF, Taylor RK (January 2000). "The type 4 prepilin peptidases comprise a novel family of aspartic acid proteases". The ...
https://en.wikipedia.org/wiki/Prepilin_peptidase
Caspase 14Caspase 14
The CASP14 gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases ... Hu S, Snipas SJ, Vincenz C, Salvesen G, Dixit VM (Dec 1998). "Caspase-14 is a novel developmentally regulated protease". J Biol ... Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two ...
https://en.wikipedia.org/wiki/Caspase_14
PlasmepsinPlasmepsin
... s are aspartic acid proteases, meaning their active site contains two aspartic acid residues. These two aspartic acid ... The name plasmepsin may come from Plasmodium (the organism) and pepsin (a common aspartic acid protease with similar molecular ... Dame JB, Reddy GR, Yowell CA, Dunn BM, Kay J, Berry C (1994). "Sequence, expression and modeled structure of an aspartic ... Bernstein NK, Cherney MM, Loetscher H, Ridley RG, James MN (1999). "Crystal structure of the novel aspartic proteinase zymogen ...
https://en.wikipedia.org/wiki/Plasmepsin
NeurodegenerationNeurodegeneration
Caspases (cysteine-aspartic acid proteases) cleave at very specific amino acid residues. There are two types of caspases: ... CAG encodes for the amino acid glutamine. A repeat of CAG results in a polyglutamine (polyQ) tract. Diseases showing this are ... 2010). "Critical role of transglutaminase and other stress proteins during neurodegenerative processes". Amino Acids. 38 (2): ... 39-43 amino acids in length, called beta-amyloid (also written as A-beta or Aβ). Beta-amyloid is a fragment from a larger ...
https://en.wikipedia.org/wiki/Neurodegenerative
Caspase 3Caspase 3
The CASP3 protein is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays ... This specificity allows caspases to be incredibly selective, with a 20,000-fold preference for aspartic acid over glutamic acid ... the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases". ... cleavage of a protein sequence to the carboxy-terminal side of an aspartic acid when it is part of a particular 4-amino acid ...
https://en.wikipedia.org/wiki/Caspase_3
Caspase 8Caspase 8
The CASP8 gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases ... the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases". Proc. ... Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. ... Boldin MP, Goncharov TM, Goltsev YV, Wallach D (June 1996). "Involvement of MACH, a novel MORT1/FADD-interacting protease, in ...
https://en.wikipedia.org/wiki/Caspase_8
Caspase 10Caspase 10
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of ... The Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases". Proc. ... The Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases". Proc. ... 1996). "Mch3, a novel human apoptotic cysteine protease highly related to CPP32". Cancer Res. 55 (24): 6045-52. PMID 8521391. ...
https://en.wikipedia.org/wiki/Caspase_10
Caspase 6Caspase 6
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of ... The Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases". Proc. ... The Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases". Proc. ... Fernandes-Alnemri T, Litwack G, Alnemri ES (Aug 1995). "Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene ...
https://en.wikipedia.org/wiki/Caspase_6
RhizopuspepsinRhizopuspepsin
... (EC 3.4.23.21, Rhizopus aspartic proteinase, neurase, Rhizopus acid protease, Rhizopus acid proteinase) is an ... "Studies on mold proteases. Part II. Substrate specificity of acid protease of Rhizopus chinensis". Agric. Biol. Chem. 33: 1419- ... Suguna K, Padlan EA, Smith CW, Carlson WD, Davies DR (October 1987). "Binding of a reduced peptide inhibitor to the aspartic ... Kurono, Y.; Chidimatsu, M.; Horikoshi, K.; Ikeda, Y. (1971). "Isolation of a protease from a Rhizopus product". Agric. Biol. ...
https://en.wikipedia.org/wiki/Rhizopuspepsin
Beta-secretase 1Beta-secretase 1
BACE1 is an aspartic acid protease important in the formation of myelin sheaths in peripheral nerve cells: in mice the ... BACE1 is distantly related to the pathogenic aspartic-acid protease plasmepsin, which is a potential target for future anti- ... membrane-associated aspartic protease 2, memapsin-2, aspartyl protease 2, and ASP2, is an enzyme that in humans is encoded by ... "Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE". Science. 286 ( ...
https://en.wikipedia.org/wiki/Beta-secretase_1
EndothiapepsinEndothiapepsin
Barkholt V (September 1987). "Amino acid sequence of endothiapepsin. Complete primary structure of the aspartic protease from ... Endothiapepsin (EC 3.4.23.22, Endothia aspartic proteinase, Endothia acid proteinase, Endothia parasitica acid proteinase, ... Hemmings AM, Foundling SI, Sibanda BL, Wood SP, Pearl LH, Blundell T (December 1985). "Energy calculations on aspartic ... Whitaker, J.R. (1970). "Protease of Endothia parasitica". Methods Enzymol. 19: 436-445. doi:10.1016/0076-6879(70)19032-x. ...
https://en.wikipedia.org/wiki/Endothiapepsin
PenicillopepsinPenicillopepsin
... (EC 3.4.23.20, peptidase A, Penicillium janthinellum aspartic proteinase, acid protease A, Penicillium citrinum ... acid proteinase, Penicillium cyclopium acid proteinase, Penicillium expansum acid proteinase, Penicillium janthinellum acid ... Penicillium roqueforti acid proteinase, Penicillium duponti aspartic proteinase, Penicillium citrinum aspartic proteinase) is ... Emi S, Myers DV, Iacobucci GA (February 1976). "Purification and properties of the thermostable acid protease of Penicillium ...
https://en.wikipedia.org/wiki/Penicillopepsin
MucorpepsinMucorpepsin
Mucor acid proteinase, Mucor acid protease, Mucor miehei aspartic proteinase, Mucor miehei aspartic protease, Mucor pusillus ... "Protein chemical characterization of Mucor pusillus aspartic proteinase. Amino acid sequence homology with the other aspartic ... doi:10.1016/0076-6879(70)19033-1. Ottesen M, Rickert W (1970). "The acid protease of Mucor miehei". Methods Enzymol. 19: 459- ... Mucorpepsin (EC 3.4.23.23, Mucor rennin, Mucor aspartic proteinase, ...
https://en.wikipedia.org/wiki/Mucorpepsin
Picornain 3CPicornain 3C
Caspase stands for cysteine-aspartic acid protease and play an essential role in the apoptotic pathway of the cell. Protease 2A ... Picornain 3C are cysteine proteases related by amino acid sequence to trypsin-like serine proteases. Picornain 3C is encoded by ... Therefore, protease 3C depends on poliovirus 3CD protein for the translocation of 3C protease to carry out transcription ... Much testing will hopefully find an effective antiviral therapy targeting 3C protease. Targeting and inhibiting 3C protease ...
https://en.wikipedia.org/wiki/Picornain_3C
RhodotorulapepsinRhodotorulapepsin
... (EC 3.4.23.26, Rhodotorula aspartic proteinase, Cladosporium acid protease, Cladosporium acid proteinase, ... Sawada, J. (1964). "The acid-protease of Paecilomyces varioti. III. The specificity of the crystalline acid-protease on ... "Studies on the acid-protease of Paecilomyces varioti Bainier TPR-220. Part I. Crystallization of the acid-protease of ... "The structure and function of acid proteases. V. Comparative studies on the specific inhibition of acid proteases by ...
https://en.wikipedia.org/wiki/Rhodotorulapepsin
ProteaseProtease
... proteases) or a water molecule (aspartic acid, metallo- and acid proteases) nucleophilic so that it can attack the peptide ... Alternatively, proteases may be classified by the optimal pH in which they are active: Acid proteases Neutral proteases ... aspartic, and metallo proteases. The threonine and glutamic-acid proteases were not described until 1995 and 2004 respectively ... using a threonine secondary alcohol Aspartic proteases - using an aspartate carboxylic acid Glutamic proteases - using a ...
https://en.wikipedia.org/wiki/Protease
Acidic proteaseAcidic protease
There are two classes of acidic proteases: Aspartic proteases - that use a catalytic aspartic acid in their active site ... Glutamic proteases - that use a catalytic glutamic acid in their active site (less common) This disambiguation page lists ... articles associated with the title Acidic protease. If an internal link led you here, you may wish to change the link to point ...
https://en.wikipedia.org/wiki/Acidic_protease
Glutamic proteaseGlutamic protease
The glutamine then returns the glutamic acid to its initial state. Aspartic protease Fujinaga M, Cherney MM, Oyama H, Oda K, ... These enzymes are acid proteases; eqolisin for example is most active at pH 2.0 when casein is used as substrate. Eqolosins ... Glutamic proteases are a group of proteolytic enzymes containing a glutamic acid residue within the active site. This type of ... belongs to subfamily of serine protease, serine-carboxyl protease (sedolisin) which was discovered in 2001. These proteases are ...
https://en.wikipedia.org/wiki/Glutamic_protease
Aspartic proteaseAspartic protease
LaPointe CF, Taylor RK (January 2000). "The type 4 prepilin peptidases comprise a novel family of aspartic acid proteases". The ... Aspartic proteases are a catalytic type of protease enzymes that use an activated water molecule bound to one or more aspartate ... Pepstatin is an inhibitor of aspartate proteases. Five superfamilies (clans) of aspartic proteases are known, each representing ... Eukaryotic aspartic proteases include pepsins, cathepsins, and renins. They have a two-domain structure, arising from ancestral ...
https://en.wikipedia.org/wiki/Aspartic_protease
Caspase 5Caspase 5
... is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of ... cysteine proteases called caspases. It is an inflammatory caspase, along with caspase 1, caspase 4 and the murine caspase 4 ...
https://en.wikipedia.org/wiki/Caspase_5
Aspartic proteaseAspartic protease
Taylor RK, LaPointe CF (2000). "The type 4 prepilin peptidases comprise a novel family of aspartic acid proteases". J. Biol. ... Eukaryotic aspartyl protease. Structure of the dimeric aspartic protease HIV protease in white and grey, with peptide substrate ... Aspartic proteases are a catalytic type of protease enzymes that use an activated water molecule bound to one or more aspartate ... Pepstatin is an inhibitor of aspartate proteases. Classification[edit]. Five superfamilies (clans) of aspartic proteases are ...
https://en.wikipedia.org/wiki/Aspartate_protease
Aspergillopepsin IAspergillopepsin I
Aspergillus acid protease, Aspergillus acid proteinase, Aspergillus aspartic proteinase, Aspergillus awamori acid proteinase, ... "The structure and function of acid proteases. VI. Effects of acid protease-specific inhibitors on the acid proteases from ... Aspergillus saitoi acid proteinase, pepsin-type aspartic proteinase, Aspergillus niger acid proteinase, sumizyme AP, proctase P ... aspartic proteinase from Aspergillus awamori. IV. Amino acid sequence of the enzyme". Bioorg. Khim. 12: 1030-1047. Yagi F, Fan ...
https://en.wikipedia.org/wiki/Aspergillopepsin_I
Proteases in angiogenesisProteases in angiogenesis
... arginine-glycine-aspartic acid) motif. Most disintegrins contain this conserved RGD motif, but ADAM15 is the only member of the ... They include serine, aspartic, and cysteine-type proteases. A highly characterized example of the serine protease family is the ... while other proteases participate in the degradation and removal of the remaining cell debris. Proteases play numerous roles in ... Proteases not only facilitate angiogenesis, but they also have the ability to put the brakes on the process. One example of ...
https://en.wikipedia.org/wiki/Proteases_in_angiogenesis
Caspase 4Caspase 4
... is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue (LEVD-), and belongs to a family ... of cysteine proteases called caspases. The function of caspase 4 is not fully known, but it is believed to be an inflammatory ...
https://en.wikipedia.org/wiki/Caspase_4
Caspase 2Caspase 2
It belongs to a family of cysteine proteases called caspases that cleave proteins only at an amino acid following an aspartic ... the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases". Proc. ... Caspase 2 has a similar amino acid sequence to initiator caspases, including caspase 1, caspase 4, caspase 5, and caspase 9. It ... Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two ...
https://en.wikipedia.org/wiki/Caspase_2
Discovery and development of HIV-protease inhibitorsDiscovery and development of HIV-protease inhibitors
Each monomer contributes an aspartic acid residue that is essential for catalysis, Asp-25 and Asp-25´. The HIV protease has the ... HIV protease inhibitors fit the active site of the HIV aspartic protease and were rationally designed utilizing knowledge of ... In common usage HIV usually implies HIV-1. HIV-1 protease is one of the best known aspartic proteases, and an attractive target ... The mechanism of the HIV protease shares many features with the rest of the aspartic protease family although the full detailed ...
https://en.wikipedia.org/wiki/Discovery_and_development_of_HIV-protease_inhibitors
Glutamyl endopeptidase IGlutamyl endopeptidase I
Proteases of this group hydrolyzes peptide bonds after the negatively charged glutamic acid or aspartic acid, with a higher ... "V8 protease". Other common references to this protease are staphylococcal serine protease, and SspA from its corresponding gene ... Glutamyl endopeptidase I is a family of extracellular bacterial serine proteases. The proteases within this family have been ... It has been shown that in spite of their similarities, the proteases from different species may differ in their efficiency in ...
https://en.wikipedia.org/wiki/Glutamyl_endopeptidase_I
Cathepsin ACathepsin A
Aspartic acid protease. *Metalloendopeptidase. *Threonine endopeptidase *Proteasome endopeptidase complex. *HslU-HslV peptidase ... 1990). "Galactosialidosis: simultaneous deficiency of esterase, carboxy-terminal deamidase and acid carboxypeptidase activities ... homology to yeast proteases". Cell. 54 (6): 755-64. doi:10.1016/S0092-8674(88)90999-3. PMID 3136930.. ...
https://en.wikipedia.org/wiki/Cathepsin_A
Chloroplast DNAChloroplast DNA
... and poor in acidic amino acids like aspartic acid and glutamic acid.[42] In an aqueous solution, the transit sequence forms a ... Chloroplast transit peptides exhibit huge variation in length and amino acid sequence.[42] They can be from 20-150 amino acids ... Tic100 is a nuclear encoded protein that's 871 amino acids long. The 871 amino acids collectively weigh slightly less than 100 ... At the N-terminal end is the A-domain, which is rich in acidic amino acids and takes up about half the protein length.[38][48] ...
https://en.m.wikipedia.org/wiki/Chloroplast_DNA
PRNPPRNP
fatal familial insomnia - aspartic acid-178 is replaced by asparagine while methionine is present at amino acid 129[51] ... The protein can exist in multiple isoforms, the normal PrPC and protease-resistant forms designated PrPRes such as the disease- ... Creutzfeldt-Jakob disease - glutamic acid-200 is replaced by lysine while valine is present at amino acid 129 ... "Nucleic Acids Res. 29 (3): 753-758. doi:10.1093/nar/29.3.753. PMC 30388. PMID 11160898.. ...
https://en.m.wikipedia.org/wiki/PRNP
Soy sauceSoy sauce
... the most abundant amino acids in Chinese soy sauce product are glutamic acid, aspartic acid, alanine and leucine.[35] ... Acid-hydrolyzed vegetable protein[edit]. Some brands of soy sauce are made from acid-hydrolyzed soy protein instead of brewed ... Lactic acid bacteria ferments the sugars into lactic acid and yeast makes ethanol, which through aging and secondary ... Acid-hydrolyzed soy sauce (산분해간장) - made by hydrolyzing raw materials containing protein with acid. ...
https://en.wikipedia.org/wiki/Kecap
TransferrinTransferrin
... and one aspartic acid. For the iron ion to bind, an anion is required, preferably carbonate (CO2−. 3).[8] ... Each monomer consists of three domains: the protease, the helical, and the apical domains. The shape of a transferrin receptor ... The amino acids which bind the iron ion to the transferrin are identical for both lobes; two tyrosines, one histidine, ... In humans, transferrin consists of a polypeptide chain containing 679 amino acids and two carbohydrate chains. The protein is ...
https://en.wikipedia.org/wiki/Transferrin
NMDA receptorNMDA receptor
Homoquinolinic acid - synthetic glutamate site partial agonist. *N-Methyl-D-aspartic acid (NMDA) - synthetic glutamate site ... The discovery of NMDA receptors was followed by the synthesis and study of N-methyl-D-aspartic acid (NMDA) in the 1960s by Jeff ... N-Methyl-D-aspartic acid (NMDA), which the NMDA receptor was named after, is a partial agonist of the active or glutamate ... N-Methyl-D-aspartic acid (NMDA), a synthetic partial agonist of the main site of the NMDAR. ...
https://en.wikipedia.org/wiki/NMDA_receptor
Protein (nutrient)Protein (nutrient)
These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids ... proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is ... The requirement for nonspecific nitrogen for the synthesis of the nutritionally dispensable amino acids (aspartic acid, ... Dietary Reference Intakes for Energy, Carbohydrate, Fiber, Fat, Fatty Acids, Cholesterol, Protein and Amino Acids, Institute of ...
https://en.m.wikipedia.org/wiki/Protein_
CaspaseCaspase
... aspartic proteases, cysteine aspartases or cysteine-dependent aspartate-directed proteases) are a family of protease enzymes ... a cysteine in its active site nucleophilically attacks and cleaves a target protein only after an aspartic acid residue. As of ... Horvitz and his colleague Junying Yuan found in 1993 that the protein encoded by the ced-3 gene is cysteine protease with ... They are named caspases due to their specific cysteine protease activity - ...
https://en.m.wikipedia.org/wiki/Caspase
NepenthesNepenthes
Amagase, S.; Nakayama, S.; Tsugita, A. (1969). "Acid protease in Nepenthes. II. Study on the specificity of nepenthesin". The ... "Enzymatic and structural characterization of nepenthesin, a unique member of a novel subfamily of aspartic proteinases" ... Schulze, W.; Frommer, W.B.; Ward, J.M. (1999). "Transporters for ammonium, amino acids and peptides are expressed in pitchers ... During pitcher development, at least 29 digestive proteins including proteases, chitinases, pathogenesis-related proteins and ...
https://en.wikipedia.org/wiki/Nepenthaceae
SoybeanSoybean
Aspartic acid. 5.112 g. Glutamic acid. 7.874 g. Glycine. 1.880 g. Proline. 2.379 g. ... For human consumption, soybeans must be cooked with "wet" heat to destroy the trypsin inhibitors (serine protease inhibitors). ... Phytic acidEdit. Main article: Phytic acid. Soybeans contain a high level of phytic acid, which has many effects including ... Saturated fatty acids (g) 0.67 0.18 0.26 0.03 0.07 0.79 0.02 0.04 0.46 0.14 minimal ...
https://en.m.wikipedia.org/wiki/Soy
Liosta Bithmhóilíní - VicipéidLiosta Bithmhóilíní - Vicipéid
Aigéad aspartach Aspartic acid. *Argainín démheitile neamhshiméadrachAsymmetric dimethylarginine. *Atrial-natriuretic peptide ( ... Aigéad éarúcach Erucic acid] - CH3(CH2)7CH=CH(CH2)11COOH ... Aigéad pailmíteach Palmitic acid. *Pantothenic acid (Vitamin B5 ... Aigéad úrach Uric acid - C5H4N4O3 ... Próitéáis Protease. *Próitéin Protein. *Proteinoid. *Putrescine ...
https://ga.wikipedia.org/wiki/Liosta_Bithmhóiliní
Proteasome endopeptidase complexProteasome endopeptidase complex
Aspartic acid protease. *Metalloendopeptidase. *Threonine endopeptidase *Proteasome endopeptidase complex. *HslU-HslV peptidase ... large multicatalytic protease, proteasome organelle, alkaline protease, 26S protease, tricorn proteinase, tricorn protease) is ... a threonine protease". Science. 268 (5210): 579-82. doi:10.1126/science.7725107. PMID 7725107.. ...
https://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex
Signal peptide peptidaseSignal peptide peptidase
Martoglio B, Golde TE (October 2003). "Intramembrane-cleaving aspartic proteases and disease: presenilins, signal peptide ... A nine amino acid-long cleavage fragment is then presented on HLA-E receptors and modulates the activity of natural killer ... a presenilin-type aspartic protease". Science. 296 (5576): 2215-8. doi:10.1126/science.1070925. PMID 12077416. ... "A C-terminal region of signal peptide peptidase defines a functional domain for intramembrane aspartic protease catalysis". J. ...
https://en.wikipedia.org/wiki/Signal_peptide_peptidase
Discovery and development of direct thrombin inhibitorsDiscovery and development of direct thrombin inhibitors
DTIs that fit in the active binding site have to fit in the hydrophobic pocket (S1) that contains aspartic acid residue at the ... Thrombin is in the serine protease family. It has 3 binding domains in which thrombin-inhibition drugs bind to. Those proteases ... Bivalirudin, a 20 amino acid polypeptide, is a synthetic analog of hirudin. Like the hirudins it is also a bivalent DTI. It has ... Thrombin also has two exosites (1 and 2). Thrombin is a little different from other serine proteases as exosite 1 is anion- ...
https://en.wikipedia.org/wiki/Discovery_and_development_of_direct_thrombin_inhibitors
Cathepsin DCathepsin D
... is an aspartic endo-protease that is ubiquitously distributed in lysosomes.[7] The main function of cathepsin D is ... The catalytic sites of cathepsin D include two critical aspartic residues (amino acid 33 and 231) located on the 14 kDa and ... The optimum pH for cathepsin D in vitro is 4.5-5.0.[13] Cathepsin-D is an aspartic protease that depends critically on ... the N-terminal segment of the protease moves out of the active site as pH drops.[14][15][16] Similar to other aspartic ...
https://en.wikipedia.org/wiki/Cathepsin_D
Protein metabolismProtein metabolism
Aspartic Acid -OOC-CH2- Oxaloacetate → Aspartic Acid (aminotransferase) Glutamic Acid -OOC-(CH2)2- α-ketoglutarate → Glutamic ... One way the body regulates proteases is through protease inhibitors. Protease inhibitors can be other proteins, small peptides ... Amino Acid SynthesisEdit. Pathways that form each amino acid[4]. Amino Acid R-group‡ Pathway* ... The main amino acids involved are serine, histidine, and aspartic acid. They all play a role in cleaving the peptide bond. ...
https://en.m.wikipedia.org/wiki/Amino_acid_metabolism
Caspase 8Caspase 8
The CASP8 gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases ... the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases". Proc. ... Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. ... Ye S, Goldsmith EJ (2002). "Serpins and other covalent protease inhibitors". Curr. Opin. Struct. Biol. 11 (6): 740-5. doi: ...
https://en.wikipedia.org/wiki/CASP8
Cysteine proteaseCysteine protease
... and the aspartic protease precursor pepsinogen. The protease is activated by removal of an inhibitory segment or protein. ... Cysteine proteases are used as feed additives for livestock to improve the digestibility of proteins and nucleic acids.[9] ... Cysteine proteases, also known as thiol proteases, are enzymes that degrade proteins. These proteases share a common catalytic ... Protease inhibitors are usually proteins with domains that enter or block a protease active site to prevent substrate access. ...
https://en.m.wikipedia.org/wiki/Cysteine_endopeptidase
Protein primary structureProtein primary structure
atom to form D-amino acids, which cannot be cleaved by most proteases. Additionally, proline can form stable trans-isomers at ... Aspartic acid Asp D Cysteine Cys C Glutamic acid Glu E Glutamine Gln Q ... Amino acids are polymerised via peptide bonds to form a long backbone, with the different amino acid side chains protruding ... 20 natural amino acid notation Amino Acid 3-Letter[4]. 1-Letter[4]. ...
https://en.m.wikipedia.org/wiki/Protein_primary_structure
Active siteActive site
HIV protease belongs to aspartic protease family and has a similar mechanism. Firstly the aspartate residue activates a water ... Acid/base catalysisEdit. In some reactions, protons and hydroxide may directly act as acid and base in term of specific acid ... HIV protease inhibitors are used to treat patients having AIDS virus by preventing its DNA replication. HIV protease is used by ... The active site usually contains non-polar amino acids, although sometimes polar amino acids may also occur.[2] The binding of ...
https://en.m.wikipedia.org/wiki/Active_site
Dipeptidyl peptidase-4Dipeptidyl peptidase-4
Aspartic acid protease. *Metalloendopeptidase. *Threonine endopeptidase *Proteasome endopeptidase complex. *HslU-HslV peptidase ... protease binding. • aminopeptidase activity. • protein binding. • serine-type endopeptidase activity. Cellular component. • ... 1x70: HUMAN DIPEPTIDYL PEPTIDASE IV IN COMPLEX WITH A BETA AMINO ACID INHIBITOR ... 2oph: Human dipeptidyl peptidase IV in complex with an alpha amino acid inhibitor ...
https://en.wikipedia.org/wiki/Dipeptidyl_peptidase-4
ProteaseProtease
... proteases) or a water molecule (aspartic acid, metallo- and acid proteases) nucleophilic so that it can attack the peptide ... aspartic, and metallo proteases.[2] The threonine and glutamic-acid proteases were not described until 1995 and 2004 ... Acid proteases. *Neutral proteases involved in type 1 hypersensitivity. Here, it is released by mast cells and causes ... Acid proteases secreted into the stomach (such as pepsin) and serine proteases present in duodenum (trypsin and chymotrypsin) ...
https://en.wikipedia.org/wiki/E1101
CaseinCasein
Chymosin (EC 3.4.23.4) is an aspartic protease that specifically hydrolyzes the peptide bond in Phe105-Met106 of κ-casein, and ... A1 and A2 beta-casein are genetic variants of the beta-casein milk protein that differ by one amino acid; a proline occurs at ... As a food source, casein supplies amino acids, carbohydrates, and two essential elements, calcium and phosphorus.[5] ... The clot is able to provide a sustained slow release of amino acids into the blood stream, sometimes lasting for several hours. ...
https://en.wikipedia.org/wiki/Casein-free_diet
Hookworm infectionHookworm infection
Three proteins have been identified: the aspartic protease-hemoglobinase APR-1, the cysteine protease-hemoglobinase CP-2, and a ... However, as red blood cell levels are restored, shortage of other essentials such as folic acid or vitamin B12 may develop, so ... "Vaccination of Dogs with a Recombinant Cysteine Protease from the Intestine of Canine Hookworms Diminishes the Fecundity and ... "Vaccination with Recombinant Aspartic Hemoglobinase Reduces Parasite Load and Blood Loss after Hookworm Infection in Dogs" ...
https://en.m.wikipedia.org/wiki/Hookworm_infection
MukorpepsinMukorpepsin
"Protein chemical characterization of Mucor pusillus aspartic proteinase. Amino acid sequence homology with the other aspartic ... Ottesen, M. and Rickert, W. (1970). "The acid protease of Mucor miehei". Methods Enzymol. 19: 459-460. ... Sternberg, M. (1972). "Bond specificity, active site and milk cloting mechanism of the Mucor miehei protease". Biochim. Biophys ...
https://sh.wikipedia.org/wiki/Mukorpepsin
Aspergillus fumigatusAspergillus fumigatus
... including those from the serine protease, aspartic protease, and metalloprotease families.[33][34][35][36] Yet, the large ... SrbA is cleaved from an endoplasmic reticulum residing 1015 amino acid precursor protein to a 381 amino acid functional form. ... proteases that cleave elastin in order to break down these macromolecular polymers for uptake. A significant correlation ... "RbdB, a Rhomboid Protease Critical for SREBP Activation and Virulence in Aspergillus fumigatus". mSphere. 1 (2). doi:10.1128/ ...
https://en.wikipedia.org/wiki/Aspergillus_fumigatus
CaspaseCaspase
... aspartic proteases, cysteine aspartases or cysteine-dependent aspartate-directed proteases) are a family of protease enzymes ... a cysteine in its active site nucleophilically attacks and cleaves a target protein only after an aspartic acid residue. As of ... 1996). "Human ICE/CED-3 Protease Nomenclature". Cell. 87 (2): 171. doi:10.1016/S0092-8674(00)81334-3. PMID 8861900.. ... Horvitz and his colleague Junying Yuan found in 1993 that the protein encoded by the ced-3 gene is cysteine protease with ...
https://en.wikipedia.org/wiki/Caspases
PlasmodiumPlasmodium
On a molecular level, the parasite damages red blood cells using plasmepsin enzymes - aspartic acid proteases which degrade ... The apicoplast is involved in isoprenoid metabolism, Fe-S cluster synthesis, fatty acid synthesis, and phospholipid ...
https://en.wikipedia.org/wiki/Antiplasmodial
PepsinPepsin
Pepstatin is a low molecular weight compound and potent inhibitor specific for acid proteases with a Ki of about 10−10 M for ... Pepsin is an aspartic protease, using a catalytic aspartate in its active site.[2] ... "Acid and non-acid reflux in patients with persistent symptoms despite acid suppressive therapy: a multicentre study using ... Weak or non-acid reflux is correlated with reflux symptoms and mucosal injury.[18][19][20][21] Under non-acid conditions ( ...
https://en.wikipedia.org/wiki/Pepsin
Cholecystokinin B receptorCholecystokinin B receptor
"Evidence for a direct interaction between the penultimate aspartic acid of cholecystokinin and histidine 207, located in the ... gastric acid secretion. • positive regulation of cytosolic calcium ion concentration. • cell surface receptor signaling pathway ... Beinborn M, Lee YM, McBride EW, Quinn SM, Kopin AS (Mar 1993). "A single amino acid of the cholecystokinin-B/gastrin receptor ... Silvente-Poirot S, Wank SA (Jun 1996). "A segment of five amino acids in the second extracellular loop of the cholecystokinin-B ...
https://en.wikipedia.org/wiki/Cholecystokinin_B_receptor
A natural variant of the cysteine protease virulence factor of group A Streptococcus with an arginine-glycine-aspartic acid ...A natural variant of the cysteine protease virulence factor of group A Streptococcus with an arginine-glycine-aspartic acid ...
A natural variant of the cysteine protease virulence factor of group A Streptococcus with an arginine-glycine-aspartic acid ( ... A natural variant of the cysteine protease virulence factor of group A Streptococcus with an arginine-glycine-aspartic acid ( ... A natural variant of the cysteine protease virulence factor of group A Streptococcus with an arginine-glycine-aspartic acid ( ... A natural variant of the cysteine protease virulence factor of group A Streptococcus with an arginine-glycine-aspartic acid ( ...
https://www.pnas.org/content/96/1/242?ijkey=84f82c089fa6418975f0cd25645e6e6c67350831&keytype2=tf_ipsecsha
Aspartic acid proteases in yeast - DTU Research DatabaseAspartic acid proteases in yeast - DTU Research Database
Aspartic acid proteases are important tools in the food industry. e.g chymosin is used in the production of cheese, and pepsin ... Only a few aspartic acid proteases are known from the yeast Saccharomyces cerevisiae, proteinase A, Bar1, yapsin 1-7. The ... Yapsins are unique among the aspartic acid proteases because of their specificity for mono- and dibasic sites.. The production ... Future uses for specific proteases include that as a tool for the processing of heterologous proteins. ...
https://orbit.dtu.dk/en/projects/aspartic-acid-proteases-in-yeast
Cell-free production of integral membrane aspartic acid proteases reveals zinc-dependent methyltransferase activity of the...Cell-free production of integral membrane aspartic acid proteases reveals zinc-dependent methyltransferase activity of the...
Cell-free production of integral membrane aspartic acid proteases reveals zinc-dependent methyltransferase activity of the ... Cell-free production of integral membrane aspartic acid proteases reveals zinc-dependent methyltransferase activity of the ...
https://www.chem.wisc.edu/content/cell-free-production-integral-membrane-aspartic-acid-proteases-reveals-zinc-dependent
Aspartic protease - WikipediaAspartic protease - Wikipedia
Taylor RK, LaPointe CF (2000). "The type 4 prepilin peptidases comprise a novel family of aspartic acid proteases". J. Biol. ... Eukaryotic aspartyl protease. Structure of the dimeric aspartic protease HIV protease in white and grey, with peptide substrate ... Aspartic proteases are a catalytic type of protease enzymes that use an activated water molecule bound to one or more aspartate ... Pepstatin is an inhibitor of aspartate proteases. Classification[edit]. Five superfamilies (clans) of aspartic proteases are ...
https://en.wikipedia.org/wiki/Aspartate_protease
Folkers, Gerd | Author | Ellibs EbookstoreFolkers, Gerd | Author | Ellibs Ebookstore
Aspartic Acid Proteases as Therapeutic Targets. Ghosh, Arun K.. 158,40€. Bioisosteres in Medicinal Chemistry. Brown, Nathan ...
https://www.ellibs.com/books/author/0/F/Folkers%2C+Gerd
Central blockade of NLRP3 reduces blood pressure via regulating inflammation microenvironment and neurohormonal excitation in...Central blockade of NLRP3 reduces blood pressure via regulating inflammation microenvironment and neurohormonal excitation in...
... and pro-cysteine aspartic acid protease-1 (pro-caspase-1). The NLRP3 inflammasome is assembled and matured under different ... Plasma norepinephrine (NE) in prehypertensive rats was increased and gamma-aminobutyric acid (GABA) was reduced. NLRP3 blockade ...
https://link.springer.com/article/10.1186/s12974-018-1131-7
Rabbit polyclonal to Caspase 3.This gene encodes a protein which is a member of the cysteine-aspartic acid protease caspase)...Rabbit polyclonal to Caspase 3.This gene encodes a protein which is a member of the cysteine-aspartic acid protease caspase)...
More from: Rabbit polyclonal to Caspase 3.This gene encodes a protein which is a member of the cysteine-aspartic acid protease ...
http://labsmart.net/tag/rabbit-polyclonal-to-caspase-3-this-gene-encodes-a-protein-which-is-a-member-of-the-cysteine-aspartic-acid-protease-caspase-family-sequential-activation-of-caspases/
Protease and Phosphatase Inhibitors | Thermo Fisher Scientific - CAProtease and Phosphatase Inhibitors | Thermo Fisher Scientific - CA
This article describes the use of protease and phosphatase inhibitors to preserve protein integrity and function after ... Aspartic acid proteases. Reversible. 1mg/mL (MeOH). 1 to 20µM. PMSF. 174.2. Serine proteases. Reversible. 18mg/mL (MeOH). 0.1 ... Our Thermo Scientific protease inhibitor cocktails and tablets target serine-, cysteine- and aspartic acid proteases and ... Serine proteases. Irreversible. 200mg/mL (H2O). 0.2 to 1.0mM. Aprotinin. 6511.5. Serine proteases. Reversible. 10mg/mL (H2O). ...
https://www.thermofisher.com/ca/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/protease-phosphatase-inhibitors.html
MEDLINE - Resultado p gina 1 MEDLINE - Resultado p gina 1
0 (Lipid Bilayers); 0 (Saposins); EC 3.4.- (Aspartic Acid Proteases). [Em] M s de entrada:. 1708. ... The present study characterized the aspartic protease saposin-like domains of four plant species, Solanum tuberosum (potato), ... cido Asp rtico Proteases/qu mica. Cynara/enzimologia. Hordeum/enzimologia. Dom nios Proteicos. Saposinas/qu mica. Solanum ... cido Asp rtico Proteases/fisiologia. Concentra o de ons de Hidrog nio. Bicamadas Lip dicas/qu mica. Estrutura Secund ria de ...
http://bases.bireme.br/cgi-bin/wxislind.exe/iah/online/?IsisScript=iah/iah.xis&nextAction=lnk&base=MEDLINE&lang=p&format=detailed.pft&indexSearch=EX&exprSearch=D08.211.790.500
MEDLINE - Resultado p gina 1 MEDLINE - Resultado p gina 1
Aspartic Acid Proteases); EC 3.4.- (Peptide Hydrolases); IY9XDZ35W2 (Glucose). ... of proteases from A1-family aspartic proteases. Compared with proteins induced by single wheat bran or soybean dregs, the ... cido Asp rtico Proteases/bioss ntese. Celulases/bioss ntese. Fibras na Dieta/an lise. Gr os Comest veis/qu mica. Fermenta o. ... Amendment damages the function of continuous flooding in decreasing Cd and Pb uptake by rice in acid paddy soil.. ...
http://bases.bireme.br/cgi-bin/wxislind.exe/iah/online/?IsisScript=iah/iah.xis&nextAction=lnk&base=MEDLINE&lang=p&format=detailed.pft&indexSearch=EX&exprSearch=A18.024.500.750.500
Neurodegeneration - WikipediaNeurodegeneration - Wikipedia
Caspases (cysteine-aspartic acid proteases) cleave at very specific amino acid residues. There are two types of caspases: ... CAG encodes for the amino acid glutamine. A repeat of CAG results in a polyglutamine (polyQ) tract. Diseases showing this are ... 2010). "Critical role of transglutaminase and other stress proteins during neurodegenerative processes". Amino Acids. 38 (2): ... 39-43 amino acids in length, called beta-amyloid (also written as A-beta or Aβ). Beta-amyloid is a fragment from a larger ...
https://en.wikipedia.org/wiki/Neurodegenerative
Protease Inhibitor Cocktail EDTA Free (ab270055) | AbcamProtease Inhibitor Cocktail EDTA Free (ab270055) | Abcam
... and aspartic acid proteases. It is supplied as a 100X… ... and aspartic acid proteases. It is supplied as a 100X stock ... Protease Inhibitor Cocktail EDTA Free (ab270055) protects protein extracts from aminopeptidases, metalloproteases, and serine, ... Pepstatin A: Reversible aspartic acid protease inhibitor. Inhibits pepsin, rennin, cathespin D, chymosin, and protease B. ... ab270055 is provided as a 100X stock solution of protease inhibitors. Dilute product into desired lysis buffer to obtain a 1X ...
https://www.abcam.com/protease-inhibitor-cocktail-edta-free-ab270055.html
Protease and Phosphatase Inhibitor Tablets | Thermo Fisher Scientific - JPProtease and Phosphatase Inhibitor Tablets | Thermo Fisher Scientific - JP
Serine and Cysteine Protease. (reversible). X. X. Pepstatin. Aspartic acid proteases. (reversible). X. ... Protease inhibition. Protease assays are somewhat confounding in that general protease substrates are not a true assessment of ... Panel A: Protease assay reactions were incubated for 2 hours and 37°C. Panel B: Acid phosphatase assay reactions were incubated ... The percent protease inhibition is indicated for each protease inhibitor formulation. #cq-image-jsp-a92f4487-31b5-44ca-8b5a- ...
https://www.thermofisher.com/jp/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/protease-phosphatase-inhibitor-tablets.html
Aspartic protease - WikipediaAspartic protease - Wikipedia
LaPointe CF, Taylor RK (January 2000). "The type 4 prepilin peptidases comprise a novel family of aspartic acid proteases". The ... Aspartic proteases are a catalytic type of protease enzymes that use an activated water molecule bound to one or more aspartate ... Pepstatin is an inhibitor of aspartate proteases. Five superfamilies (clans) of aspartic proteases are known, each representing ... Eukaryotic aspartic proteases include pepsins, cathepsins, and renins. They have a two-domain structure, arising from ancestral ...
https://en.wikipedia.org/wiki/Aspartic_protease
PepsinPepsin
Proteases: aspartic acid proteases (EC 3.4.23). Vertebrate. Pepsin · Chymosin · Renin · Signal peptide peptidase · Beta ... Pepsin is a digestive protease (EC 3.4.23.1) released by the chief cells in the stomach that functions to degrade food proteins ... Other important digestive proteases are the pancreatic enzymes trypsin and chymotrypsin. Pepsin denatures if the pH is more ... Pepsin is expressed as a pro-form zymogen, pepsinogen, whose primary structure has an additional 44 amino acids. ...
https://www.bionity.com/en/encyclopedia/Pepsin.html
Cathepsin D | Harvard Catalyst Profiles | Harvard CatalystCathepsin D | Harvard Catalyst Profiles | Harvard Catalyst
Aspartic Acid Proteases [D08.811.277.656.074]. *Aspartic Acid Endopeptidases [D08.811.277.656.074.500] ...
https://connects.catalyst.harvard.edu/Profiles/display/Concept/Cathepsin%20D
HIV ProteaseHIV Protease
HIV proteases are aspartic acid proteases and thus, aspartate 25 plays a key role in binding the substrate. ... HIV Protease. What is a Protease?. Proteases are basically proteins that break down other proteins. They cleave the peptide ... and protease. Thus, an HIV specific protease is necessary for the the HIV to make more functional viruses. The HIV protease is ... Proteases also have many functions. Proteases such as pepsin and trypsin break down proteins in food so that it can be absorbed ...
http://community.middlebury.edu/~sontum/chemistry/students/kim/protease.html
Neutralizing monoclonal antibody against Dickkopf2 impairs lung cancer progression via activating NK cells | Cell Death...Neutralizing monoclonal antibody against Dickkopf2 impairs lung cancer progression via activating NK cells | Cell Death...
4c). The cleaved caspase3 protein is a member of the cysteine-aspartic acid protease family36,37. Sequential activation of ...
https://www.nature.com/articles/s41420-019-0204-4?error=cookies_not_supported&code=136be76a-d45e-4302-b02c-e593b87c0761
What are protein hydrolyzing enzymes known as? | Reference.comWhat are protein hydrolyzing enzymes known as? | Reference.com
About.com Chemistry specifically defines protease as an enzyme that initiates the hydrolysis of a peptide bond to form ... Proteases are classified into groups: serine, threonine, cysteine, aspartic acid, glutamic acid and metalloproteases. Threonine ... Proteases can break individual peptide bonds or a complete peptide down to amino acids. ... Protein hydrolyzing enzymes are known as protease. About.com Chemistry specifically defines protease as an enzyme that ...
https://www.reference.com/science/protein-hydrolyzing-enzymes-known-8bb766a4a5415ca9
Exam 4 - Antivirals (Medchem-Stahelin) Flashcards by | BrainscapeExam 4 - Antivirals (Medchem-Stahelin) Flashcards by | Brainscape
aspartic protease. (has aspartic acid in active site) 73 HIV protease inhibitors are _______mimetics. and can of what 2 types? ... HIV Protease is an ______ protease. (aka has a _____ in the active site) ... is a protease inhibitor if given at low doses is used to increase serum concentrations of other antivirals ... 4. nucleic acid synthesis. 5. integration / transcription. 6. viral protein synthesis. 7. packaging/assembly. 8. viral release ...
https://www.brainscape.com/flashcards/exam-4-antivirals-medchem-stahelin-7638751/packs/12357771
In vitro apoptotic and DNA damaging potential of nanobarium oxide | IJNIn vitro apoptotic and DNA damaging potential of nanobarium oxide | IJN
Cysteine-aspartic acid protease-3 activity. Cells (5×105 cells/well) were spread on a culture plate (96 well) and incubated at ... Cysteine-aspartic acid protease-3 enzyme and apoptosis. Caspase-3 enzyme shows main activity in apoptotic cells, which was ... the role of Cysteine-aspartic acid protease-3 increased in a dose- and duration-dependent manner. ... Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95: ...
https://www.dovepress.com/in-vitro-apoptotic-and-dna-damaging-potential-of-nanobarium-oxide-peer-reviewed-fulltext-article-IJN
Amyloid-beta precursor protein secretase - WikipediaAmyloid-beta precursor protein secretase - Wikipedia
BACE is a transmembrane protein with an extracellular aspartic acid protease domain. γ-secretase is actually a protein complex ... Presenilin is believed to harbor the protease domain and represents an important example of an uncommon type of protease that ...
https://en.wikipedia.org/wiki/Amyloid-beta_precursor_protein_secretase
Serine proteaseSerine protease
... serine proteases or serine endopeptidases (newer name) are a class of peptidases (enzymes that cleave peptide bonds in ... Serine proteases - Cysteine protease - Aspartic acid protease - Metalloendopeptidases. Cathepsin 3.4.18,21,22,23. A - B - C - K ... "serine protease") and aspartic acid (Asp 102). Located very near one another near the heart of the enzyme, these three key ... It was discovered that additional amino acids of the protease, Gly 193 and Ser 195, are involved in creating what is called an ...
https://www.bionity.com/en/encyclopedia/Serine_protease.html
test #8 3.21 Flashcards by Nashid Chaudhury | Brainscapetest #8 3.21 Flashcards by Nashid Chaudhury | Brainscape
able to cleave aspartic acid residues (cysteine-aspartic acid-proteases). initiators activate effectors. ... homogentisic acid accummulates and excreted in urine. turns black if allowed to stand & oxidize. homogentisic acid deposition ... sulfur-containing amino acid synthesized from amino acid serine and homocysteine (which is derived from methionine) ... blue-black pigmentation most evident on ears, nose, and cheeks (homogentisic acid deposition in collagen of CT, tendon, and ...
https://www.brainscape.com/flashcards/test-8-321-2115476/packs/3729903
Generic Caspase Activity Assay Kit (Fluorometric - Red) (ab112131) | AbcamGeneric Caspase Activity Assay Kit (Fluorometric - Red) (ab112131) | Abcam
Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central ... Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits ...
https://www.abcam.com/generic-caspase-activity-assay-kit-fluorometric-red-ab112131.html
β-Secretase Cleavage of Alzheimers Amyloid Precursor Protein by the Transmembrane Aspartic Protease BACE | Scienceβ-Secretase Cleavage of Alzheimer's Amyloid Precursor Protein by the Transmembrane Aspartic Protease BACE | Science
Each motif contains the highly conserved signature sequence of aspartic proteases, DT/S G T/S, within which the aspartic acid ... These proteases are prime therapeutic targets. A transmembrane aspartic protease with all the known characteristics of β- ... Numbers on the left indicate amino acid position; boxes, conserved aspartic protease active site domains; asterisks, predicted ... We now report the cloning of a human transmembrane aspartic protease, BACE, that has all the known characteristics of the β- ...
https://science.sciencemag.org/content/286/5440/735?ijkey=a076c7e49be3e4dc4184d0099ee950f3562974f3&keytype2=tf_ipsecsha
Autophagic and Apoptotic Effects of HDAC Inhibitors on Cancer CellsAutophagic and Apoptotic Effects of HDAC Inhibitors on Cancer Cells
In both pathways, activated caspases (cysteine aspartic acid-specific proteases) cleave their substrates and activate other ... Hydroxamic acids (TSA, SAHA, LAQ824, and CBHA) and pyroxamic acids (PXD101 and CRA-026440) are pan-HDAC inhibitors targeting ... P. Munster, D. Marchion, E. Bicaku et al., "Clinical and biological effects of valproic acid as a histone deacetylase inhibitor ... S. Yamamoto, K. Tanaka, R. Sakimura et al., "Suberoylanilide hydroxamic acid (SAHA) induces apoptosis or autophagy-associated ...
https://www.hindawi.com/journals/bmri/2011/830260/
CASP14 gene - Genetics Home Reference - NIHCASP14 gene - Genetics Home Reference - NIH
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a ... Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two ...
https://ghr.nlm.nih.gov/gene/CASP14
CASP7 | Cancer Genetics WebCASP7 | Cancer Genetics Web
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a ... We analyzed the cytotoxic effect of a 17-amino acid synthetic peptide (s-cal14.1a) that is based on a native toxin (cal14.1a) ... aspartic-type endopeptidase activity - cellular component disassembly involved in execution phase of apoptosis - cysteine-type ... Previously we isolated a type of ω-9 polyunsaturated fatty acid (JA) from the bark of J. mandshurica, however little is known ...
http://www.cancerindex.org/geneweb/CASP7.htm
CASP8 Gene - GeneCards | CASP8 Protein | CASP8 AntibodyCASP8 Gene - GeneCards | CASP8 Protein | CASP8 Antibody
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a ... This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a ... Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. ... Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. ...
https://www.genecards.org/cgi-bin/carddisp.pl?gene=CASP8&keywords=GH02J201471&prefilter=genomic_location
ResiduesCaspasesCleavageCaspaseEnzymesProteinsEnzymeSubstrateInhibitorsProteinSerine ProteaseCleavesEndopeptidasesTrypsinTransmembrane aspartic proteaseAspartateAmino acidTargetsInhibitionPancreaticMetalloproteasesSpecific proteaseBroad-spectrum proteaseExtracellularAprotininPeptidasePepsinGlutamicBeta-secretaseRole of proteasesEDTAPepstatinAspartyl proteasesMediatesReversibleIrreversibleMammalianOrganismsBACE1Nucleic AcidsCleave the peptideCatalyticPolypeptide
Residues16
  • Aspartic proteases are a catalytic type of protease enzymes that use an activated water molecule bound to one or more aspartate residues for catalysis of their peptide substrates. (wikipedia.org)
  • Aspartyl proteases are a highly specific family of proteases - they tend to cleave dipeptide bonds that have hydrophobic residues as well as a beta-methylene group. (wikipedia.org)
  • While a number of different mechanisms for aspartyl proteases have been proposed, the most widely accepted is a general acid-base mechanism involving coordination of a water molecule between the two highly conserved aspartate residues. (wikipedia.org)
  • These amino acid residues form much of the connective tissues in meat). (bionity.com)
  • The pocket that is in "trypsin" and "chymotrypsin" is now partially filled with valine and threonine , rendering it a mere depression, which can accommodate these smaller amino acid residues. (bionity.com)
  • Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. (abcam.com)
  • Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. (genecards.org)
  • The protease is a homodimer, whose active site consists of two apposed aspartic acid residues. (rcsb.org)
  • Integrase core domain contains the D-x(n)-D-x(35)-E motif, named for the phylogenetically conserved glutamic acid and aspartic acid residues and the invariant 35 amino acid spacing between the second and third acidic residues. (rcsb.org)
  • A large proportion of the absolutely conserved residues in aspartic proteinases are polar and buried. (rcsb.org)
  • Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. (lsbio.com)
  • Site-directed mutagenesis at 42 positions of the IPNV VP4 protein was performed to determine the active site and the important residues for the protease activity. (asm.org)
  • Granzyme B, similarly to the caspases, cleaves its substrates after aspartic acid residues, suggesting that this protease has the ability to activate members of the caspase family directly. (genome.jp)
  • Caspase-6 (Cysteine-Requiring Aspartate Proteases) is part of a family of intracellular cysteine proteases that cleave their substrates after aspartic acid residues. (acris-antibodies.com)
  • Procaspase-6 (Mch2), a member of the ICE/ced-3 subfamily, is an inactive proenzyme that is activated to form caspase-6 by proteolytic cleavage at certain aspartic acid residues. (acris-antibodies.com)
  • DFFA is encoded by an alternatively encrypted mRNAs originating two distinct forms: short (ICAD-S) and long (ICAD-L), which act like a specific chaperone ensuring the correct CAD's folding Besides, it contains two aspartic acid residues (Asp117 and Asp224) where CAD is identified and, consequently, it stays bounded until Caspase-3 splits this union. (wikipedia.org)
Caspases12
  • Rabbit polyclonal to Caspase 3.This gene encodes a protein which is a member of the cysteine-aspartic acid protease caspase) family.Sequential activation of caspases. (labsmart.net)
  • Caspases are members of the cysteine-aspartic acid protease (caspase) family. (abcam.com)
  • Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. (genecards.org)
  • Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. (genecards.org)
  • Caspases (cysteinyl aspartate proteases) are involved in the signaling pathways of apoptosis, necrosis and inflammation. (genecards.org)
  • Proteases of the ICE/Ced-3 family (caspases) ( 1 ) are activated during the apoptotic response, including that activated by chemotherapeutic drugs, and cleave specific protein substrates. (asm.org)
  • Caspases are cysteine-containing aspartic acid-specific proteases which exist as zymogens in the soluble cytoplasm, endoplasmic reticulum (ER), mitochondrial intermembrane space, and nuclear matrix [10]. (thefreedictionary.com)
  • One of the key initiation elements of the apoptotic pathway is the activation of caspases (cysteinyl-aspartic acid proteases) followed by cleavage of the caspase substrates [1]. (bio-medicine.org)
  • Caspases, or cysteine-aspartic proteases or cysteine-dependent aspartate-directed proteases are a family of cysteine proteases that play essential roles in apoptosis (programmed cell death), necrosis, and inflammation. (agscientific.com)
  • This balance is achieved, in part, through the precise regulation of apoptosis, which involves complex molecular events that ultimately activate, or prevent the activation of caspases (cysteine‐aspartic acid proteases). (els.net)
  • Apoptosis is a genetically programmed process for the elimination of damaged or redundant cells by activation of caspases (aspartate-specific cysteine proteases). (genome.jp)
  • The proteases that mediate apoptosis are called caspases (cysteinyl-aspartic acid proteases). (biocare.net)
Cleavage15
  • Proposed mechanism of peptide cleavage by aspartyl proteases. (wikipedia.org)
  • Unlike serine or cysteine proteases these proteases do not form a covalent intermediate during cleavage. (wikipedia.org)
  • Most known proteases belong to one of four evolutionarily distinct enzyme families based on the functional groups involved in cleavage of the peptide bond. (thermofisher.com)
  • Proteases are divided into two broad categories based on cleavage site and then subdivided based on the active site and pH preferences. (thermofisher.com)
  • Cleavage of the cysteine protease substrate results in fluorescence at 460nm, and cleavage of the serine protease results in fluorescence at 520nm. (thermofisher.com)
  • Aβ generation depends on proteolytic cleavage of the amyloid precursor protein (APP) by two unknown proteases: β-secretase and γ-secretase. (sciencemag.org)
  • Overexpression of this protease, termed BACE (for beta-site APP-cleaving enzyme) increased the amount of β-secretase cleavage products, and these were cleaved exactly and only at known β-secretase positions. (sciencemag.org)
  • 35.) Vassar R, Bennett BD, Babu-Khan S, Kahn S, Mendiaz EA, Denis P Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. (thefreedictionary.com)
  • Cleavage of peptide bonds lead to degradation of protein substrates into their constituent amino acids, or it can be specific, leading to selective protein cleavage for post-translational modification and processing. (scielo.br)
  • We present a novel mechanism for specific substrate cleavage involving several dynamic processes including positive cooperativity and homotropic allostery for this interesting class of intramembrane proteases. (embopress.org)
  • Intermembrane proteases of the rhomboid family, thought to have broad substrate specificity and to only partly rely on cleavage recognition motifs in substrates, exhibit considerable substrate specificity due to positive cooperativity and allosteric substrate activation. (embopress.org)
  • Here we show that the protein responsible for cleavage of this motif is plasmepsin V (PMV), an aspartic acid protease located in the endoplasmic reticulum. (edu.au)
  • The results suggest that VP4 cleaves the (Ser/Thr)-X-Ala↓(Ser/Ala)-Gly motif, a target sequence with similarities to bacterial leader peptidases and herpesvirus protease cleavage sites. (asm.org)
  • Cleavage of caspase-3 requires aspartic acid at the P1 position. (biocare.net)
  • Moreover, several gamma-secretase inhibitors block this same step in Notch processing, indicating that related protease activities are responsible for cleavage within the predicted transmembrane domains of Notch and APP. (nih.gov)
Caspase9
  • Upon activation, NLRP3 inflammasome is formed when NLRP3 recruits the adapter protein apoptosis-associated speck-like protein (ASC) and pro-cysteine aspartic acid protease-1 (pro-caspase-1). (springer.com)
  • This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. (nih.gov)
  • Jung, Choi, Lee, Kim, Hwang, Choi: Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis. (antikoerper-online.de)
  • Caspase-7 antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human Caspase-7.The immunogen is located within amino acids 30 - 80 of Caspase-7. (genetex.com)
  • CASP3 is part of the cysteine-aspartic acid protease (caspase) family. (creativebiomart.net)
  • The aspartic acid specific protease caspase-9 has been linked to the mitochondrial death pathway. (creativebiomart.net)
  • CASP14 caspase 14, apoptosis-related cysteine peptidase BackgroundCaspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis.1 The precursor form of all. (craftstylish.com)
  • Cleaved caspase-3 antibody detects endogenous levels of the large fragment of activated caspase-3, a protease that mediates apoptosis. (biocare.net)
  • CASP1 / Caspase 1 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. (lsbio.com)
Enzymes23
  • In modern-day enzymes, although the three-dimensional structures are very similar, the amino acid sequences are more divergent, except for the catalytic site motif, which is very conserved. (wikipedia.org)
  • Proteases and phosphatases are important enzymes in a variety of biochemical pathways in living cells. (thermofisher.com)
  • All living organisms contain proteolytic enzymes (proteases and peptidases). (thermofisher.com)
  • Thus, while numerous compounds have been identified and used to inactivate or block these enzymes, no single chemical is effective for all types of proteases and phosphatases (see table). (thermofisher.com)
  • Proteases are a ubiquitous class of enzymes that hydrolyze protein peptide bonds. (thermofisher.com)
  • Other important digestive proteases are the pancreatic enzymes trypsin and chymotrypsin . (bionity.com)
  • The gag precurser will eventually give rise to structural proteins and pol precurser will give rise to enzymes such as reverse transcriptase, integrase, and protease. (middlebury.edu)
  • In biochemistry , serine proteases or serine endopeptidases (newer name) are a class of peptidases ( enzymes that cleave peptide bonds in proteins ) that are characterised by the presence of a serine residue in the active site of the enzyme . (bionity.com)
  • Serine proteases participate in a wide range of functions in the body, including blood clotting , immunity , and inflammation , as well as contributing to digestive enzymes in both prokaryotes and eukaryotes. (bionity.com)
  • The triad is located in the active site of the enzyme, where catalysis occurs, and is preserved in all serine protease enzymes. (bionity.com)
  • Protein hydrolyzing enzymes are known as protease. (reference.com)
  • This hydrolytic activity is given by a family of enzymes and zymogens present in the cell, called widely 'proteases', with the purpose to hydrolyze the peptide bond [7,21]. (thefreedictionary.com)
  • Proteases of fungal origin can be produced cost effectively, have an advantage faster production, the ease with which the enzymes can be modified and mycelium can be easily removed by filtration. (scielo.br)
  • The production of proteases has been carried out using submerged fermentation, but conditions in solid state fermentation lead to several potential advantages for the production of fungal enzymes. (scielo.br)
  • This review focuses on the production of fungal proteases, their distribution, structural-functional aspects, physical and chemical parameters, and the use of these enzymes in industrial applications. (scielo.br)
  • Microbial proteases are among the most important hydrolytic enzymes and have been studied extensively. (scielo.br)
  • Fungal proteases have attracted the attention of environmental biotechnologists because fungi can grow on low cost substrates and secrete large amount of enzymes into culture medium which could ease downstream processing ( Anitha and Palanivelu, 2013 ). (scielo.br)
  • In this present review, some aspects of fungal proteolytic enzymes are discussed, with reference to the production of protease, their distribution and their industrial applications. (scielo.br)
  • Proteases (peptidases or proteolytic enzymes) constitute a large group of enzymes that catalyse the hydrolysis of peptide bonds in other proteins. (scielo.br)
  • Proteases are classified as peptide hydrolases or peptidases (EC 3.4) and constitute a large family of enzymes, divided into endopeptidases (EC 3.4.21-99) and exopeptidases (EC 3.4.11-19), classified according to the position of the peptide bond to be cleaved. (scielo.br)
  • The crystal structure of a mammalian aspartic proteinase indicates that interactions with substrate may be more extensive on the prime side of the active site cleft than in the fungal enzymes and involve Tyr189 and the loop 290 to 295, perhaps contributing to the transpeptidase activity of pepsin and the specificity of the renins. (rcsb.org)
  • Proteases that catalyse the hydrolysis of peptide bonds constitute the most abundant group of enzymes. (embopress.org)
  • In addition to this substitution, a number of viral enzymes (also found in adenovirus and some picornaviruses) utilize glutamic acid, instead of the catalytic aspartic acid ( 9 , 22 ). (asm.org)
Proteins21
  • Future uses for specific proteases include that as a tool for the processing of heterologous proteins. (dtu.dk)
  • In whole cells, protease and phosphatase activities are tightly regulated by compartmentalization or inhibitors to prevent indiscriminate damage to cellular proteins and to maintain proper function of signaling pathways. (thermofisher.com)
  • Disruption of cellular and tissue architecture during protein extraction distorts the in vivo state by making all proteins potentially accessible for degradation or modification by endogenous proteases and phosphatases. (thermofisher.com)
  • Protease and phosphatase inhibitors can be added to the lysis reagents in order to prevent degradation of extracted proteins, and to obtain the best possible protein yield and activity following cell lysis. (thermofisher.com)
  • The preservation of proteins from protease and phosphatase activities after cell or tissue lysis is essential for many research applications. (thermofisher.com)
  • Our formulations protected proteins from protease and phosphatase degradation and performed favorably compared to other commercial preparations. (thermofisher.com)
  • Pepsin is a digestive protease ( EC 3.4.23.1) released by the chief cells in the stomach that functions to degrade food proteins into peptides . (bionity.com)
  • Proteases are basically proteins that break down other proteins. (middlebury.edu)
  • Proteases such as pepsin and trypsin break down proteins in food so that it can be absorbed into the blood for further metabolism. (middlebury.edu)
  • Certain proteases destroy harmful proteins. (middlebury.edu)
  • These protein precurser, Gag and Gag Pol must be cleaved by protease at 9 specific points in order to produce functional proteins. (middlebury.edu)
  • The omnipresence of proteases and their natural inhibitors has placed them among the most studied proteins in biochemistry. (ucsf.edu)
  • Common secondary 3-dimensional structure of proteins in which the linear sequence of amino acids is folded into a spiral that is stabilized by hydrogen bonds between the carboxyl oxygen of each peptide bond. (tripod.com)
  • Proteases hydrolyze the peptide bonds of proteins into peptides and amino acids, being found in all living organisms, and are essential for cell growth and differentiation. (scielo.br)
  • Intramembrane proteases are polytopic membrane proteins that cleave transmembrane substrates and, similar to soluble proteases, are known to play roles in essential biological processes (Wolfe, 2009 ). (embopress.org)
  • An aspartyl protease directs malaria effector proteins to the host cell. (edu.au)
  • A feature of exported proteins is a pentameric motif (RxLxE/Q/D) that is a substrate for an unknown protease. (edu.au)
  • The proteolytic processing of viral precursor proteins is a crucial step in the life cycle of a majority of viruses that infect eucaryotic cells, and virus-encoded proteases are generally associated with these events. (asm.org)
  • tasp-1 encodes the C. elegans homolog of Taspase, a threonine-aspartic acid protease that has been found, in both mammals and insects, to cleave several proteins involved in transcription, in particular MLL1/trithorax and TFIIA. (g3journal.org)
  • Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. (goldbio.com)
  • Our microplate readers answer your questions on nucleic acids: concentration, interaction with other nucleic acids or proteins, single nucleotide polymorphisms and expression of genes. (bmglabtech.com)
Enzyme5
  • The HIV Protease is an enzyme with two symmetrical subunits. (middlebury.edu)
  • Located very near one another near the heart of the enzyme, these three key amino acids each play an essential role in the cleaving ability of the proteases. (bionity.com)
  • About.com Chemistry specifically defines protease as an enzyme that initiates the hydrolysis of a peptide bond to form polypeptide chains. (reference.com)
  • The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. (genecards.org)
  • A panel of eleven human cancer cell lines, glioblastoma and carcinoma, were exposed to serial dilutions of ascorbic acid (5-100 mmol/L). The purpose of this study was to analyse the impact of catalase, an important hydrogen peroxide-detoxifying enzyme, on the resistance of cancer cells to ascorbic acid mediated oxidative stress. (biomedcentral.com)
Substrate5
  • Structure of the dimeric aspartic protease HIV protease in white and grey, with peptide substrate in black and active site aspartate side chains in red. (wikipedia.org)
  • [5] [6] One aspartate activates the water by abstracting a proton, enabling the water to perform a nucleophilic attack on the carbonyl carbon of the substrate scissile bond , generating a tetrahedral oxyanion intermediate stabilized by hydrogen-bonding with the second aspartic acid. (wikipedia.org)
  • Pancreatic extract (50 μL, 1 μg/μL protein) or trypsin (25 μL, 0.1 units/μL) was incubated with a quenched-fluorescent, protease-cleavable substrate for cysteine (A) or serine proteases (B) in the presence or absence of commercially available protease inhibitors with EDTA-containing (blue) or EDTA-free (purple) formulations. (thermofisher.com)
  • HIV proteases are aspartic acid proteases and thus, aspartate 25 plays a key role in binding the substrate. (middlebury.edu)
  • Chemical biological approaches are used or are being developed to study the substrate specificity, catalytic mechanism and biological role of proteases and their macromolecular inhibitors. (ucsf.edu)
Inhibitors15
  • Protease and phosphatase inhibitors are essential components of most cell lysis and protein extraction procedures. (thermofisher.com)
  • Protease inhibitors are biological or chemical compounds that function by reversibly or irreversibly binding to the protease. (thermofisher.com)
  • Proteases inhibitors are nearly always needed, while phosphatase inhibitors are required only when phosphorylation states (activation states) are being investigated. (thermofisher.com)
  • Particular research experiments may necessitate the use of single inhibitors or customized mixtures, but most protein work is best served by using a suitable protease inhibitor cocktail (see additional discussion below table). (thermofisher.com)
  • It is supplied as a 100X stock solution of protease inhibitors dissolved in DMSO. (abcam.com)
  • ab270055 is provided as a 100X stock solution of protease inhibitors. (abcam.com)
  • My research focuses on structure-function analysis of proteases and their inhibitors. (ucsf.edu)
  • The research efforts of the lab focus primarily on the structure-function analysis of proteases and their inhibitors using a combination of genetic, biochemical and biophysical methods. (ucsf.edu)
  • The genes for serine (trypsin, serine collagenase, easter, prostate specific antigen, T-cell specific granzymes, MT-SP1 and 2, KSHV protease), cysteine (cruzain and falcipain) and aspartyl (HIV1 & HIV2 & SIV) proteases and macromolecular inhibitors such as ecotin and single chain antibodies are manipulated and expressed in heterologous expression systems. (ucsf.edu)
  • San Diego, CA, February 19, 2016 --(PR.com)-- Jared Cumming, Director, Global Chemistry at Merck, will give a distinguished speaker presentation on, "Discovery of Verubecestat (MK-8931): A beta-Secretase Inhibitor in Phase 3 Clinical Development for Alzheimer's Disease" at GTCbio's 2nd Protease Inhibitors in Drug Discovery Conference to be held on March 1-2, 2016 in San Diego, CA. (thefreedictionary.com)
  • Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. (fishersci.ca)
  • Thermo Scientific™ Halt Protease Inhibitor Cocktails are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction procedures. (fishersci.ca)
  • Halt Protease Inhibitor Cocktails are 100X solutions containing optimized concentrations of six broad-spectrum protease inhibitors stabilized in high-quality dimethylsulfoxide (DMSO). (fishersci.ca)
  • Several successful strategies have been applied towards the development of currently in use protease inhibitors to treat hypertension, HIV, cancer, diabetes and coagulation disorders (Drag & Salvesen, 2010 ). (embopress.org)
  • Discovery of Selective Matriptase and Hepsin Serine Protease Inhibitors: Useful Chemical Tools for Cancer Cell Biology. (ucsf.edu)
Protein11
  • Protease Inhibitor Cocktail EDTA Free (ab270055) protects protein extracts from aminopeptidases, metalloproteases, and serine, cysteine, and aspartic acid proteases. (abcam.com)
  • Ab270055 is a general-purpose reagent suitable for protease inhibition during protein extraction from mammalian tissues and cells, yeast and fungal cells, and bacteria. (abcam.com)
  • The Thermo Scientific Pierce Protease, Phosphatase, and combination Protease and Phosphatase Inhibitor Tablets are broad-spectrum formulations ideal for preventing proteolytic degradation and dephosphorylation during cell lysis and protein extraction. (thermofisher.com)
  • BACE is a transmembrane protein with an extracellular aspartic acid protease domain. (wikipedia.org)
  • We suggest therefore that alterations in isoform diversity or transcript expression levels within the major venom protein families are likely to be responsible for prey specificity, rather than differences in the representation of entire toxin families or the recruitment of novel toxin families, although the recruitment of lysosomal acid lipase as a response to vertebrate feeding cannot be excluded. (biomedcentral.com)
  • Fungal proteases are used for hydrolyzing protein and other components of soy beans and wheat in soy sauce production. (scielo.br)
  • The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. (nih.gov)
  • The computed amino acid sequences of the skipper ORFs contain regions resembling retrotransposon polyprotein domains, including a nucleic acid binding protein, aspartyl protease, reverse transcriptase and integrase. (elsevier.com)
  • Recombinant protein corresponding to amino acids of human CASP8. (abnova.com)
  • Therefore, current therapeutic strategies aimed at decreasing BACE1 protein levels should consider the role of this protease in copper homeostasis. (mcgill.ca)
  • The polyprotein, whose protein order is NH 2 -pVP2-VP4-VP3-COOH, is cotranslationally processed and cleaved at the pVP2-VP4 and the VP4-VP3 junctions by the VP4 (also named NS)-associated protease activity ( 20 , 21 ). (asm.org)
Serine Protease4
  • Irreversible serine protease inhibitor. (abcam.com)
  • 80% of serine protease activity. (thermofisher.com)
  • Subtilisin is a serine protease in prokaryotes . (bionity.com)
  • histidine (His 57), serine (Ser 195) (hence the name "serine protease") and aspartic acid (Asp 102). (bionity.com)
Cleaves2
  • Pepsin cleaves the 44 amino acids from pepsinogen to create more pepsin. (bionity.com)
  • Presenilin is believed to harbor the protease domain and represents an important example of an uncommon type of protease that cleaves targets within the cell membrane. (wikipedia.org)
Endopeptidases3
  • [1] More recently, aspartic endopeptidases associated with the processing of bacterial type 4 prepilin [2] and archaean preflagellin have been described. (wikipedia.org)
  • Many eukaryotic aspartic endopeptidases (MEROPS peptidase family A1) are synthesised with signal and propeptides . (wikipedia.org)
  • endopeptidases (serine, aspartic, cysteine and metalloproteinases) cleave the peptide distal to the terminus. (thermofisher.com)
Trypsin4
  • After evaluating many fluorescent substrates, we chose assay substrates for cysteine proteases (papain) and serine proteases (trypsin) and used mouse pancreatic extract (1mg/mL) as the positive control. (thermofisher.com)
  • The three serine proteases of the chymotrypsin-like clan that have been studied in greatest detail are chymotrypsin , trypsin , and elastase . (bionity.com)
  • Trypsin is responsible for cleaving peptide bonds following a positively-charged amino acid residue. (bionity.com)
  • In detail, enterokinase was added to homogenate to activate pancreatic zymogens before determination of pancreatic trypsin and protease. (thefreedictionary.com)
Transmembrane aspartic protease2
  • A transmembrane aspartic protease with all the known characteristics of β-secretase was cloned and characterized. (sciencemag.org)
  • BACE1 is a transmembrane aspartic protease (beta-secretase) found in the Golgi apparatus, trans-Golgi network, secretory vesicles, and endosomes. (thefreedictionary.com)
Aspartate1
  • Pepstatin is an inhibitor of aspartate proteases. (wikipedia.org)
Amino acid6
  • CAG encodes for the amino acid glutamine . (wikipedia.org)
  • Chymotrypsin is responsible for cleaving peptide bonds following a bulky hydrophobic amino acid residue. (bionity.com)
  • Elastase is responsible for cleaving peptide bonds following a small neutral amino acid residue, such as Alanine , glycine and valine. (bionity.com)
  • does not participate in amino acid or mRNA binding. (brainscape.com)
  • Aminopeptidases (EC 3.4.14) act at a free N terminus of the polypeptide chain and liberate a single amino acid residue, a dipeptide, or a tripeptide. (scielo.br)
  • Retroviruses, such as human immunodeficiency virus, have an active aspartic protease which is only generated when two identical amino acid domains, each of them bearing a catalytic aspartic acid, join to create a homodimer ( 35 ). (asm.org)
Targets2
  • These proteases are prime therapeutic targets. (sciencemag.org)
  • The aspartic proteases of many pathogens represent attractive targets for inhibitor design to control the progression of these diseases. (axonmedchem.com)
Inhibition1
  • We compared protease inhibition by the Pierce Tablet formulations (Table 1) to other commercial formulations. (thermofisher.com)
Pancreatic1
  • The polyprotein of infectious pancreatic necrosis virus (IPNV), a birnavirus, is processed by the viral protease VP4 (also named NS) to generate three polypeptides: pVP2, VP4, and VP3. (asm.org)
Metalloproteases2
  • ab270055 does not contain the chelating agent EDTA and therefore its effectiveness against metalloproteases is limited by comparison to protease preparations that do contain EDTA. (abcam.com)
  • Compared to the three other types of proteases, serine , cysteine , and metalloproteases , aspartic proteases comprise a relatively small group. (axonmedchem.com)
Specific protease1
  • Thus, an HIV specific protease is necessary for the the HIV to make more functional viruses. (middlebury.edu)
Broad-spectrum protease1
  • Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets broad-spectrum protease and pghospahatase inhibitor formulation provides complete protection from dephosphorylation and proteolytic degradation. (fishersci.com)
Extracellular4
  • The human pathogenic bacterium group A Streptococcus produces an extracellular cysteine protease [streptococcal pyrogenic exotoxin B (SpeB)] that is a critical virulence factor for invasive disease episodes. (pnas.org)
  • GAS isolates produce a highly conserved extracellular cysteine protease known as streptococcal pyrogenic exotoxin B (SpeB) (reviewed in ref. 5 ). (pnas.org)
  • Proteases are required for many cellular functions, including cellular repair and the digestion of extracellular material. (thermofisher.com)
  • Furthermore, due to the accumulation of lactic acid from Warburg's effect, the acidification of extracellular microenvironment favors the progression and metastases of tumor via upregulation of metallo-proteinase and cysteine proteinase activity and secretion [ 3 ]. (hindawi.com)
Aprotinin1
  • The Pierce Protease and Phosphatase Inhibitor Mini Tablets contain aprotinin, bestatin, E-64, EDTA, and leupeptin to provide protection from degradation by serine-proteases, cysteine-proteases, aspartic acid-proteases. (fishersci.com)
Peptidase2
  • A protease is also known as a peptidase or proteinase. (reference.com)
  • A representative group of novel venom transcripts exhibiting similarity to lysosomal acid lipase were identified from the E. coloratus transcriptome, whilst novel metallopeptidases exhibiting similarity to neprilysin and dipeptidyl peptidase III were identified from E. p. leakeyi and E. coloratus respectively. (biomedcentral.com)
Pepsin2
  • Pepsin is expressed as a pro-form zymogen, pepsinogen , whose primary structure has an additional 44 amino acids . (bionity.com)
  • Pepsin will digest up to 20% of ingested carbon bonds by cleaving preferentially after the N-terminal of aromatic amino acids such as phenylalanine and tyrosine . (bionity.com)
Glutamic2
  • HIV-1 protease - a major drug-target for treatment of HIV Plasmepsin - a group of aspartyl proteases found in the Malaria-causing parasite Plasmodium Glutamic protease The Proteolysis Map Fusek M, Mares M, Vetvicka V (2013-01-01). (wikipedia.org)
  • Glutamic acid was not specified until 2004. (reference.com)
Beta-secretase1
  • The "sheddase" beta-secretase (also known as the aspartic acid protease BACE1) catalyzes the first step in the production of amyloid beta (Aβ) peptides, which are central players in the pathology of Alzheimer disease. (mcgill.ca)
Role of proteases1
  • Characterization of the role of proteases in disease and also their precise mechanism of action was essential for these drug discoveries. (embopress.org)
EDTA2
  • It was previously called Proteoloc™ Protease Inhibitor Cocktail EDTA Free. (abcam.com)
  • Protease inhibitor cocktails with EDTA include separate 0.5M EDTA solution. (fishersci.ca)
Pepstatin1
  • Nearly all known aspartyl proteases are inhibited by pepstatin . (wikipedia.org)
Aspartyl proteases1
  • Retroviral and retrotransposon proteases ( retroviral aspartyl proteases ) are much smaller and appear to be homologous to a single domain of the eukaryotic aspartyl proteases. (wikipedia.org)
Mediates1
  • The aspartyl protease activity mediates proteolytic cleavages of Gag and Pol polyproteins. (rcsb.org)
Reversible2
  • Reversible inhibitor of serine and cystine proteases. (abcam.com)
  • Reversible aspartic acid protease inhibitor. (abcam.com)
Irreversible1
  • Irreversible inhibitor of cystine proteases. (abcam.com)
Mammalian2
  • The HIV protease is not found in mammalian cells. (middlebury.edu)
  • Ascorbic acid (vitamin C), an essential nutrient for mammalian cells, acts as a cofactor of different enzymatic reactions, e.g. collagen synthesis. (biomedcentral.com)
Organisms2
  • however, the variety, distribution and compartmentalization of proteases vary among the different organisms. (thermofisher.com)
  • Occurring naturally in all organisms, proteases are involved in physiological reactions. (reference.com)
BACE12
  • An aspartic-acid protease encoded by BACE1 on chromosome 11q23.2. (thefreedictionary.com)
  • BACE1 depletion almost completely abolishes Aβ production, rendering this protease a prime target to attenuate amyloid production. (mcgill.ca)
Nucleic Acids1
Cleave the peptide1
  • They cleave the peptide bonds that link amino acids together. (middlebury.edu)
Catalytic4
  • Most of the virus-encoded proteases are related to the chymotrypsin-like cellular proteases with a distinctive double β-barrel fold, but only a few, like the Alphavirus capsid protease and the Flaviviridae NS3 proteases, contain the His-Asp-Ser catalytic triad found in the cellular world ( 14 , 31 ). (asm.org)
  • A large number of viral proteases structurally related (or not) to chymotrypsin-like proteases replace the serine to cysteine as the catalytic nucleophile, as for picornavirus 3C protease or adenovirus protease ( 1 , 9 ). (asm.org)
  • The human cytomegalovirus (HCMV) protease consists of a single β-barrel structure and presents a catalytic triad His-His-Ser ( 26 ). (asm.org)
  • What is more, combining C3's amino acids leads to 5 α helices, 4 β lamina and a loop at the catalytic C-terminal which interact with each other. (wikipedia.org)
Polypeptide1
  • Recombinant Human CASP3 produced in E.Coli is a double, non-glycosylated, polypeptide chain cotaning 258 amino acids and having a molecular mass of 29.7 kDa. (creativebiomart.net)
Aspartic acid proteases in yeast - DTU Research Database
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Neurodegeneration - Wikipedia
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CASP12 Antibody (Center) - Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) WB, IHC-P, FC, IF, E - Buy Now! |Abgent
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Thermo Scientific™ Halt™ Protease Inhibitor Cocktails
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Thermo ScientificPierce Protease and Phosphatase Inhibitor Mini Tablets,EDTA-free | Fisher Scientific
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