Enzymes of the transferase class that catalyze the conversion of L-aspartate and 2-ketoglutarate to oxaloacetate and L-glutamate. EC 2.6.1.1.
The 4-aminomethyl form of VITAMIN B 6. During transamination of amino acids, PYRIDOXAL PHOSPHATE is transiently converted into pyridoxamine phosphate.
A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
An enzyme that catalyzes the conversion of L-alanine and 2-oxoglutarate to pyruvate and L-glutamate. (From Enzyme Nomenclature, 1992) EC 2.6.1.2.
An enzyme that catalyzes the conversion of L-TYROSINE and 2-oxoglutarate to 4-hydroxyphenylpyruvate and L-GLUTAMATE. It is a pyridoxal-phosphate protein. L-PHENYLALANINE is hydroxylated to L-tyrosine. The mitochondrial enzyme may be identical with ASPARTATE AMINOTRANSFERASES (EC 2.6.1.1.). Deficiency of this enzyme may cause type II Tyrosinemia (see TYROSINEMIAS). EC 2.6.1.5.
This is the active form of VITAMIN B 6 serving as a coenzyme for synthesis of amino acids, neurotransmitters (serotonin, norepinephrine), sphingolipids, aminolevulinic acid. During transamination of amino acids, pyridoxal phosphate is transiently converted into pyridoxamine phosphate (PYRIDOXAMINE).
An enzyme that catalyzes the conversion of carbamoyl phosphate and L-aspartate to yield orthophosphate and N-carbamoyl-L-aspartate. (From Enzyme Nomenclature, 1992) EC 2.1.3.2.
One of the non-essential amino acids commonly occurring in the L-form. It is found in animals and plants, especially in sugar cane and sugar beets. It may be a neurotransmitter.
A set of opposing, nonequilibrium reactions catalyzed by different enzymes which act simultaneously, with at least one of the reactions driven by ATP hydrolysis. The results of the cycle are that ATP energy is depleted, heat is produced and no net substrate-to-product conversion is achieved. Examples of substrate cycling are cycling of gluconeogenesis and glycolysis pathways and cycling of the triglycerides and fatty acid pathways. Rates of substrate cycling may be increased many-fold in association with hypermetabolic states resulting from severe burns, cold exposure, hyperthyroidism, or acute exercise.
A group of compounds that are derivatives of phenylpyruvic acid which has the general formula C6H5CH2COCOOH, and is a metabolite of phenylalanine. (From Dorland, 28th ed)
A PYRIDOXAL PHOSPHATE containing enzyme that catalyzes the transfer amino group from L-TRYPTOPHAN to 2-oxoglutarate in order to generate indolepyruvate and L-GLUTAMATE.
A PYRIDOXAL PHOSPHATE containing enzyme that catalyzes the reversible transamination of branched-chain AMINO ACIDS to 2-oxoglutarate.
A pyridoxal-phosphate protein that catalyzes the conversion of L-tyrosine to tyramine and carbon dioxide. The bacterial enzyme also acts on 3-hydroxytyrosine and, more slowly, on 3-hydroxyphenylalanine. (From Enzyme Nomenclature, 1992) EC 4.1.1.25.
A broad-spectrum excitatory amino acid antagonist used as a research tool.
An enzyme that converts brain gamma-aminobutyric acid (GAMMA-AMINOBUTYRIC ACID) into succinate semialdehyde, which can be converted to succinic acid and enter the citric acid cycle. It also acts on beta-alanine. EC 2.6.1.19.
Amino acids which have a branched carbon chain.
Blood tests that are used to evaluate how well a patient's liver is working and also to help diagnose liver conditions.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
An aspartate aminotransferase found in MITOCHONDRIA.
A spectrum of clinical liver diseases ranging from mild biochemical abnormalities to ACUTE LIVER FAILURE, caused by drugs, drug metabolites, and chemicals from the environment.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The number of PLATELETS per unit volume in a sample of venous BLOOD.
A nucleoside antimetabolite antiviral agent that blocks nucleic acid synthesis and is used against both RNA and DNA viruses.
INFLAMMATION of the LIVER in humans that is caused by HEPATITIS C VIRUS lasting six months or more. Chronic hepatitis C can lead to LIVER CIRRHOSIS.
Liver disease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules.
Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.
An aspartate aminotransferase found in the CYTOPLASM.
An enzyme that catalyzes the hydrolysis of ACETYLCHOLINE to CHOLINE and acetate. In the CNS, this enzyme plays a role in the function of peripheral neuromuscular junctions. EC 3.1.1.7.
The sum of the weight of all the atoms in a molecule.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
A publication issued at stated, more or less regular, intervals.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
Includes relationships between hospitals, their governing boards, and administrators in regard to physicians, whether or not the physicians are members of the medical staff or have medical staff privileges.
NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.
Organized services to provide information on any questions an individual might have using databases and other sources. (From Random House Unabridged Dictionary, 2d ed)
Specialized health care, supportive in nature, provided to a dying person. A holistic approach is often taken, providing patients and their families with legal, financial, emotional, or spiritual counseling in addition to meeting patients' immediate physical needs. Care may be provided in the home, in the hospital, in specialized facilities (HOSPICES), or in specially designated areas of long-term care facilities. The concept also includes bereavement care for the family. (From Dictionary of Health Services Management, 2d ed)
Facilities or services which are especially devoted to providing palliative and supportive care to the patient with a terminal illness and to the patient's family.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.

Activities of citrate synthase, NAD+-linked and NADP+-linked isocitrate dehydrogenases, glutamate dehydrogenase, aspartate aminotransferase and alanine aminotransferase in nervous tissues from vertebrates and invertebrates. (1/2887)

1. The activities of citrate synthase and NAD+-linked and NADP+-linked isocitrate dehydrogenases were measured in nervous tissue from different animals in an attempt to provide more information about the citric acid cycle in this tissue. In higher animals the activities of citrate synthase are greater than the sum of activities of the isocitrate dehydrogenases, whereas they are similar in nervous tissues from the lower animals. This suggests that in higher animals the isocitrate dehydrogenase reaction is far-removed from equilibrium. If it is assumed that isocitrate dehydrogenase activities provide an indication of the maximum flux through the citric acid cycle, the maximum glycolytic capacity in nervous tissue is considerably greater than that of the cycle. This suggest that glycolysis can provide energy in excess of the aerobic capacity of the tissue. 2. The activities of glutamate dehydrogenase are high in most nervous tissues and the activities of aspartate aminotransferase are high in all nervous tissue investigated. However, the activities of alanine aminotransferase are low in all tissues except the ganglia of the waterbug and cockroach. In these insect tissues, anaerobic glycolysis may result in the formation of alanine rather than lactate.  (+info)

Blockade of type beta transforming growth factor signaling prevents liver fibrosis and dysfunction in the rat. (2/2887)

We eliminated type beta transforming growth factor (TGF-beta) signaling by adenovirus-mediated local expression of a dominant-negative type II TGF-beta receptor (AdCATbeta-TR) in the liver of rats treated with dimethylnitrosamine, a model of persistent liver fibrosis. In rats that received a single application of AdCATbeta-TR via the portal vein, liver fibrosis as assessed by histology and hydroxyproline content was markedly attenuated. All AdCATbeta-TR-treated rats remained alive, and their serum levels of hyaluronic acid and transaminases remained at low levels, whereas all the AdCATbeta-TR-untreated rats died of liver dysfunction. The results demonstrate that TGF-beta does play a central role in liver fibrogenesis and indicate clearly in a persistent fibrosis model that prevention of fibrosis by anti-TGF-beta intervention could be therapeutically useful.  (+info)

Enrichment of canalicular membrane with cholesterol and sphingomyelin prevents bile salt-induced hepatic damage. (3/2887)

These studies were undertaken to characterize the role of plasma membrane cholesterol in canalicular secretory functions and hepatocyte integrity against intravenous taurocholate administration. Cholesterol and sphingomyelin concentrations and cholesterol/phospholipid ratios were significantly increased in canalicular membranes of diosgenin-fed rats, suggesting a more resistant structure against solubilization by taurocholate. During taurocholate infusion, control rats had significantly decreased bile flow, whereas diosgenin-fed animals maintained bile flow. Maximal cholesterol output increased by 176% in diosgenin-fed rats, suggesting an increased precursor pool of biliary cholesterol in these animals. Maximal phospholipid output only increased by 43% in diosgenin-fed rats, whereas bile salt output remained at control levels. The kinetics of glutamic oxalacetic transaminase, lactic dehydrogenase, and alkaline phosphatase activities in bile showed a significantly faster release in control than in diosgenin-fed rats. After 30 min of intravenous taurocholate infusion, necrotic hepatocytes were significantly increased in control animals. Preservation of bile secretory functions and hepatocellular cytoprotection by diosgenin against the intravenous infusion of toxic doses of taurocholate was associated with an increased concentration of cholesterol and sphingomyelin in the canalicular membrane. The increase of biliary cholesterol output induced by diosgenin was correlated to the enhanced concentration of cholesterol in the canalicular membrane.  (+info)

Phase I and pharmacokinetic study of the topoisomerase II catalytic inhibitor fostriecin. (4/2887)

We conducted a phase I and pharmacokinetic study of the topoisomerase II catalytic inhibitor fostriecin. Fostriecin was administered intravenously over 60 min on days 1-5 at 4-week intervals. Dose was escalated from 2 mg m(-2) day(-1) to 20 mg m(-2) day(-1) in 20 patients. Drug pharmacokinetics was analysed with high performance liquid chromatography with UV-detection. Plasma collected during drug administration was tested in vitro for growth inhibition of a teniposide-resistant small-cell lung cancer (SCLC) cell line. The predominant toxicities were elevated liver transaminases (maximum common toxicity criteria (CTC) grade 4) and serum creatinine (maximum CTC grade 2). These showed only a limited increase with increasing doses, often recovered during drug administration and were fully reversible. Duration of elevated alanine-amino transferase (ALT) was dose-limiting in one patient at 20 mg m(-2). Other frequent toxicities were grade 1-2 nausea/vomiting, fever and mild fatigue. Mean fostriecin plasma half-life was 0.36 h (initial; 95% CI, 0-0.76 h) and 1.51 h (terminal; 95% CI, 0.41-2.61 h). A metabolite, most probably dephosphorylated fostriecin, was detected in plasma and urine. No tumour responses were observed, but the plasma concentrations reached in the patients were insufficient to induce significant growth inhibition in vitro. The maximum tolerated dose (MTD) has not been reached, because drug supply was stopped at the 20 mg m(-2) dose level. However, further escalation seems possible and is warranted to achieve potentially effective drug levels. Fostriecin has a short plasma half-life and longer duration of infusion should be considered.  (+info)

Influences of Kupffer cell stimulation and suppression on immunological liver injury in mice. (5/2887)

AIM: To study the possible involvement of Kupffer cells (KC) in immunological liver injury in mice. METHODS: Liver injury was induced by i.v. injection of Bacillus Calmette-Guerin (BCG) 5 x 10(7) viable bacilli followed by i.v. injection of lipopolysaccharides (LPS) 7.5 micrograms to each mouse. Indian ink and silica were i.v. injected to suppress KC and retinol was given po to stimulate KC in these mice. Plasma alanine aminotransferase (AlaAT), aspatate aminotransferase (AspAT), nitric oxide (NO), and liver tissue were examined. RESULTS: Injection of LPS following BCG injection resulted in a remarkable elevation of plasma NO, AlaAT, and AspAT levels, and severe liver damage. The damages were enhanced by the activation of KC with retinol and reduced by suppression of KC with silica and Indian ink. CONCLUSION: The degree of liver injury induced by BCG + LPS is closely correlated with the status of KC, and NO from KC plays an important role in the pathogenesis of the liver damage in mice.  (+info)

Effect of epidermal growth factor on cultured rat hepatocytes poisoned by CCl4. (6/2887)

AIM: To study the effects of epidermal growth factor (EGF) on CCl4-induced primary cultured hepatocytes injury. METHODS: Alanine amino-transferase (AlaAT) and aspartate aminotransferase (AspAT) activities and K+ concentractions were determined by the Auto-biochemistry Assay System. Malondialdehyde (MDA) was determined by thiobarbituric acid method. Radioactivity was determined by liquid scintillometry. Light microscopy and electron microscopy were used. RESULTS: EGF 40 micrograms.L-1 decreased CCl4 (10 mmol.L-1)-induced damages of rat primary cultured hepatocytes by decreasing AlaAT and AspAT leakage and MDA production, and promoted RNA and DNA synthesis, with a high positive correlation between intracellular K+ leakage and DNA syntheses (r = 0.99, P < 0.01). Cytopathological study showed that EGF decreased damage of liver cells. CONCLUSION: EGF maintains the stability of cellular lipid membrane and promotes syntheses of RNA and DNA of hepatocytes, and intracellular K+ transference is a promotor of the message transmission of DNA synthesis.  (+info)

Opposite behavior of two isozymes when refolding in the presence of non-ionic detergents. (7/2887)

GroEL has a greater affinity for the mitochondrial isozyme (mAAT) of aspartate aminotransferase than for its cytosolic counterpart (cAAT) (Mattingly JR Jr, Iriarte A, Martinez-Carrion M, 1995, J Biol Chem 270:1138-1148), two proteins that share a high degree of sequence similarity and an almost identical spatial structure. The effect of detergents on the refolding of these large, dimeric isozymes parallels this difference in behavior. The presence of non-ionic detergents such as Triton X-100 or lubrol at concentrations above their critical micelle concentration (CMC) interferes with reactivation of mAAT unfolded in guanidinium chloride but increases the yield of cAAT refolding at low temperatures. The inhibitory effect of detergents on the reactivation of mAAT decreases progressively as the addition of detergents is delayed after starting the refolding reaction. The rate of disappearance of the species with affinity for binding detergents coincides with the slowest of the two rate-limiting steps detected in the refolding pathway of mAAT. Limited proteolysis studies indicate that the overall structure of the detergent-bound mAAT resembles that of the protein in a complex with GroEL. The mAAT folding intermediates trapped in the presence of detergents can resume reactivation either upon dilution of the detergent below its CMC or by adding beta-cyclodextrin. Thus, isolation of otherwise transient productive folding intermediates for further characterization is possible through the use of detergents.  (+info)

Folate nutriture alters choline status of women and men fed low choline diets. (8/2887)

Choline and folate share methylation pathways and, in studies of rats, were shown to be metabolically inter-related. To determine whether choline status is related to folate intake in humans, we measured the effect of controlled folate depletion and repletion on the plasma choline and phosphatidylcholine concentrations of 11 healthy men (33-46 y) and 10 healthy women (49-63 y) fed low-choline diets in two separate metabolic unit studies. Total folate intake was varied by supplementing low folate (25 and 56 microg/d for men and women, respectively) and low choline (238 and 147 mg/d for men and women, respectively) diets with pteroylglutamic acid for 2-6 wk following folate-depletion periods of 4-5 wk. The low folate/choline intakes resulted in subclinical folate deficiencies; mean plasma choline decreases of 28 and 25% in the men and women, respectively; and a plasma phosphatidylcholine decrease of 26% in the men (P < 0. 05). No functional choline deficiency occurred, as measured by serum transaminase and lipid concentrations. The decreases in choline status measures returned to baseline or higher upon moderate folate repletion and were more responsive to folate repletion than plasma folate and homocysteine. Feeding methionine supplements to the men did not prevent plasma choline depletion, indicating that folate is a more limiting nutrient for these methylation pathways. The results indicate that 1) choline is utilized as a methyl donor when folate intake is low, 2) the de novo synthesis of phosphatidylcholine is insufficient to maintain choline status when intakes of folate and choline are low, and 3) dietary choline is required by adults in an amount > 250 mg/d to maintain plasma choline and phosphatidylcholine when folate intake is low.  (+info)

Define glutamic oxaloacetic transaminase. glutamic oxaloacetic transaminase synonyms, glutamic oxaloacetic transaminase pronunciation, glutamic oxaloacetic transaminase translation, English dictionary definition of glutamic oxaloacetic transaminase. Noun 1. glutamic oxaloacetic transaminase - an enzyme involved in transamination glutamic oxalacetic transaminase aminopherase, aminotransferase,...
INTRODUCTION. Glutamic oxaloacetic transaminase (GO-T) is widely distributed in animal and human tissues. Its greatest activity has been found in homogenates of heart muscle, skeletal muscle, brain, liver and kidney, the relative activities varying in different species (table 1). This enzyme activity has been found in all animal and human sera tested, and the activities are comparable whether determined chromatographically,1 spectrophotometrically2 or colorimetrically3 (figure 1). It has been demonstrated that serum. ...
Recombinant Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2) Protein (His tag). Species: Human. Source: Escherichia coli (E. coli). Order product ABIN1981684.
Searcy, G.K.; Senter, S.D.; Wilson, R.L., 1995: Glutamic-Oxaloacetic Transaminase Activity: A Potential End-Point-Temperature Indicator for Imported Cooked Beef †
TY - JOUR. T1 - Aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) detection techniques. AU - Huang, Xing Jiu. AU - Choi, Yang Kyu. AU - Im, Hyung Soon. AU - Yarimaga, Oktay. AU - Yoon, Euisik. AU - Kim, Hak Sung. PY - 2006/1/1. Y1 - 2006/1/1. N2 - The levels of aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) in serum can help people diagnose body tissues especially the heart and the liver are injured or not. This article provides a comprehensive review of research activities that concentrate on AST/GOT and ALT/GPT detection techniques due to their clinical importance. The detection techniques include colorimetric, spectrophotometric, chemiluminescence, chromatography, fluorescence and UV absorbance, radiochemical, and electrochemical techniques. We devote the most attention on experimental principle. In some methods a few representative devices and important conclusions are presented.. AB - The levels of aspartate aminotransferase (AST/GOT) ...
Aspartate transaminase (AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or serum glutamic oxaloacetic transaminase (SGOT), is a pyridoxal phosphate (PLP)-dependent transaminase enzyme (EC 2.6.1.1) that was first described by Arthur Karmen and colleagues in 1954. AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate and, as such, is an important enzyme in amino acid metabolism. AST is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells. Serum AST level, serum ALT (alanine transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health. The tests are part of blood panels. Aspartate transaminase catalyzes the interconversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. Aspartate (Asp) + α-ketoglutarate ↔ oxaloacetate + glutamate (Glu) As a prototypical transaminase, AST relies on PLP (Vitamin B6) as a cofactor to transfer the amino group from ...
AV Fasano, BA Kasprow, JT Velardo. Glutamic oxalacetic and glutamic pyruvic transaminase activity in the pineal gland of the albino rat during the estrous cycle. J Am Osteopath Assoc 1972;72(2):185. doi: .. Download citation file:. ...
The following laboratory values obtained within 30 prior to study entry:. Absolute neutrophil count (ANC) ≥750/mm3 Hematocrit ≥30 Platelet count ≥40,000/mm3 Calculated creatinine clearance (CrCl) ≥50 mL/min, as estimated by the Cockcroft-Gault equation* aspartate amino transferase (AST) serum glutamic oxalacetic transaminase (SGOT), amino alanine transferase (ALT) serum glutamic-pyruvic transaminase (SGPT), and alkaline phosphatase ,3 x upper limit of normal (ULN) total bilirubin ≤2.5 x ULN. NOTE: If the potential subject is taking an atazanavir-containing regimen at the time of screening, total bilirubin ≤5 x ULN is acceptable. * Calculation for the Cockcroft-Gault equation is available at https://www.fstrf.org/common/utilities/calculators/ccc.html. ...
Age greater than 18 years.. Life expectancy of greater than 3 months.. Performance status (Eastern Cooperative Oncology Group (ECOG)) less than or equal to 2.. Patients must have adequate organ and marrow function (as defined below). Patients must have returned to base line or grade one from any acute toxicity related to prior therapy.. Laboratory Test/Required Value:. Absolute neutrophil count greater than 1,500/microl.. Platelets greater than 100,000/microl.. International normalized ratio (INR) less than or equal to 1.5 times upper limit of normal (ULN) or. Partial thromboplastin time (PTT) abnormality can be explained by the presences of lupus anticoagulant or in the therapeutic range if on anticoagulation.. Total bilirubin less than or equal to 1.5 times institutional upper limits of normal.. Aspartate aminotransferase (AST)/serum glutamic oxaloacetic transaminase(SGOT) and alanine aminotransferase (ALT)/serum glutamic pyruvic transaminase(SGPT) less than or equal to 3 times institutional ...
Recently there have been news stories on a dengue strain that affects the liver. This condition is also called dengue hepatitis. It leads to severe organ malfunction and in some cases even organ failure. In the case of dengue hepatitis, fever lasts longer, often for more than a week, and leaves the patient extremely exhausted. An indication of this condition, according to experts is the sudden rise in SGOT (Serum Glutamic Oxaloacetic Transaminase) and SGPT (Serum Glutamic Pyruvic Transaminase) levels, sometime almost 20 times higher than the normal level. Dengue hepatitis is characterized by high fever, lasting more than a week, extreme weakness. The blood tests show an increase in SGPT and SGOT levels, a drop in platelet count and WBCs ...
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A study of the colorimetric dinitrophenyl-hydrazine method for the determination of serum glutamic oxalacetic transaminase activity.:
Reference: Polikarpova L.I., Effect of protein synthesis inhibitors on the hormonal induction of alanine and aspartate aminotransferases in the liver of sexually mature male rats, Voprosy meditsinskoi khimii, 1974, vol: 20(2), 215-217 ...
The severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) spread rapidly all over the world in late 2019 and caused critical illness and death in some infected patients. This study aimed at examining several laboratory factors, especially inflammatory and immunological mediators, to identify severity and mortality associated biomarkers. Ninety-three hospitalized patients with confirmed coronavirus disease 2019 (COVID-19) were classified based on disease severity. The levels of biochemical, hematological, immunological, and inflammatory mediators were assessed, and their association with severity and mortality were evaluated. Hospitalized patients were mostly men (77.4%) with an average (standard deviation) age of 59.14 (14.81) years. The mortality rate was significantly higher in critical patients (85.7%). Increased serum levels of blood sugar, urea, creatinine, uric acid, phosphorus, total bilirubin, serum glutamic-oxaloacetic transaminase, serum glutamic-oxaloacetic transaminase, lactic ...
The patient underwent an uneventful induction of anesthesia with midazolam, sodium thiopental, pancuronium, and metocurine. After induction, the patient was placed in a right flexed lateral decubitus position on a padded table with the kidney bolster raised. All pressure points were padded. Anesthesia was maintained with a combination of nitrous oxide, isoflurane, and intravenous fentanyl. The patient had no episodes of hypotension and had a urine output , 0.75 ml *symbol* kg sup -1 *symbol* h sup -1. The patient remained in a lateral position for 7 h during the surgical procedure. The long duration was due to extensive scarring surrounding the kidney. The patient did not complain of flank or hip pain in the recovery room. Biochemical testing was performed on discharge of the patient from the recovery room because of periods of renal ischemia during the partial nephrectomy. The results were remarkable for an increased serum glutamic oxaloacetic transaminase (SGOT) of 95 u/l (normal , 35 u/l) and ...
Objectives: A non-invasive, simple and non-expensive test to predict cirrhosis would be highly desirable. The aspartate aminotransferase/ alanine aminotransferase (AST/ALT) ratio has been proven to be such an indicator of cirrhosis in alcoholic liver disease, hepatitis C. Aim: To test whether the AST/ALT ratio is a marker of cirrhosis also in patients with primary biliary cirrhosis (PBC). Methods: The study consisted of 160 patients. In 126 patients, we had clinical and laboratory data at the time of diagnosis and follow-up with outcome: liver-related death, liver transplantation and survival. In 121 patients, we had laboratory data and liver histology. Results: We found that the AST/ALT ratio was significantly higher in cirrhotic patients than in non-cirrhotic patients. A high AST/ALT ratio was significantly associated with esophageal varices and ascites. In a multivariate analysis, bilirubin and ALP were predictors of poor prognosis. Conclusion: The AST/ALT ratio seems to be of clinical value ...
Fingerprint Dive into the research topics of Organ specificity of glucocorticoid-sensitive tyrosine aminotransferase. Separation from aspartate aminotransferase isoenzymes. Together they form a unique fingerprint. ...
The MaxDiscovery Aspartate Transaminase (AST) Color Endpoint Assay Kit is a plate-based colorimetric enzymatic assay for the determination of the aspartate transaminase enzyme in serum samples. Aspartate transaminase (AST) also known as aspartate aminotransferase or (sGOT) is a metabolic enzyme expressed primarily in the liver. Elevation of AST levels is an indication of liver damage and has been associated with liver injury. AST levels are monitored routinely in patients with liver diseases. AST is also a very useful tool for preclinical investigation of experimental drug formulations and AST levels are commonly used to monitor and attenuate the hepatotoxic effects of experimental drugs in rodents. The MaxDiscovery Alanine Transaminase (AST) Color Endpoint Assay Kit uses a colored reaction scheme to detect AST enzymatic activity in serum samples. In this method aspartate and -ketoglutarate are first converted to glutamate and oxaloacetate. The oxaloacetate then reacts with diazonium salt to ...
TY - JOUR. T1 - Subcellular distribution, and physical and immunological properties of hepathic alanine. T2 - Glyoxylate aminotransferase isoenzymes in different mammalian species. AU - Takada, Yoshikazu. AU - Noguchi, Tomoo. PY - 1982. Y1 - 1982. N2 - 1. 1. Subcellular distribution, and physical and immunological properties of hepatic alanine: glyoxylate aminotransferase isoenzymes 1 and 2 were examined with ten different mammalian species. 2. 2. Intracellular organelles containing isoenzyme 1 varied from species to species; isoenzyme 1 was located in the peroxisomes, mitochondria or both organelles. In contrast, isoenzyme 2 was found only in the mitochondria but not in the peroxisomes. 3. 3. In any species, isoenzyme 1 had molecular weight of approx. 80,000 with two identical subunits, and isoenzyme 2 approx. 200,000 with four identical subunits. 4. 4. In any species, an immunological cross-reactivity was observed among isoenzymes 1 and among isoenzymes 2 but did not between isoenzymes 1 and ...
TY - JOUR. T1 - Comparison of Circulating Endothelial Cell/Platelet Count Ratio to Aspartate Transaminase/Platelet Ratio Index for Identifying Patients with Cirrhosis. AU - Sethi, Saurabh. AU - Simonetto, Douglas. AU - Abdelmoneim, Soha S.. AU - Campion, Michael B.. AU - Kaloiani, Irakli. AU - Clayton, Amy C.. AU - Kremers, Walter K. AU - Halling, Kevin C.. AU - Kamath, Patrick Sequeira. AU - Talwalkar, Jayant. AU - Shah, Vijay. PY - 2012. Y1 - 2012. N2 - Background/Objectives: Circulating endothelial cells (CECs) are indicative of vascular injury and correlate with severity of vascular diseases. A pilot study showed that the ratio of CEC to platelet count (CEC/PC) was effective in predicting cirrhosis. Therefore, we evaluated CEC/PC in a larger cohort of patients, correlated it with cirrhosis, and compared its operating characteristics with previously described biomarker for cirrhosis, the AST/platelet ratio index (APRI). Methods: Fifty-three patients with cirrhosis, 20 matched healthy ...
Site: ovarian; stage: COG stage II and III, FIGO stage IC, II and III; histology: must contain at least one of the following: yolk sac tumor, embryonal carcinoma, or choriocarcinoma (pure or mixed); age (years) ,= 11 and - Site: testicular; stage: COG stage II-IV, AJCC stage II, III, International Germ Cell Consensus Classification (IGCCC) good risk; histology: must contain at least one of the following: yolk sac tumor, embryonal carcinoma, or choriocarcinoma (pure or mixed); tumor markers: for IGCCC good risk: alpha-FP = 11 and - Site: extragonadal; stage: COG stage II; histology: must contain at least one of the following: yolk sac tumor, embryonal carcinoma, or choriocarcinoma (pure or mixed) age (years) ,= 11 and 10. Patients must have a performance status corresponding to Eastern Cooperative Oncology Group (ECOG) scores of 0, 1 or 2; use Karnofsky for patients , 16 years of age and Lansky for patients =11. Serum glutamic-oxaloacetic transaminase (SGOT) (aspartate aminotransferase [AST]) or ...
METHODS: Twenty patients of age 57±11-year-old contributed with 20 clinically healthy implants and teeth. They were instructed to use an individual stent in the selected elements prior to performing oral hygiene for 14 days. For each element plaque index (PlI), probing depth (PD), bleeding on probing (Bops) were reported at 0 days and 14 days of plaque accumulation. Aspartate aminotransferase activity was measure at both time points from the crevicular fluid ...
Aminotransferase (AST) is an enzyme thats present in various parts of your body. Your doctor can order an AST test to check the level of aspartate aminotransferase in your blood. Abnormal results may be a sign of liver damage or disease. Learn more about this test.
Abstract Glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) activity were measured in precutaneous needle biopsy specimens of human liver tissue and co..
1B4X: The role of residues outside the active site: structural basis for function of C191 mutants of Escherichia coli aspartate aminotransferase.
GOT1 - GOT1 (Myc-DDK-tagged)-Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1) available for purchase from OriGene - Your Gene Company.
Aspartate aminotransferase to platelet ratio index and sustained virologic response are associated with progression from hepatitis C associated liver cirrhosis to hepatocellular carcinoma after treatment with pegylated interferon plus ribavirin Khai-Jing Ng,1,2,* Chih-Wei Tseng,1–4,* Ting-Tsung Chang,5,6 Shinn-Jia Tzeng,7 Yu-Hsi Hsieh,1,2 Tsung-Hsing Hung,1,2 Hsiang-Ting Huang,8 Shu-Fen Wu,9 Kuo-Chih Tseng1,2 1Department of Internal Medicine, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Chia-Yi, 2School of Medicine, Tzu Chi University, Hualien, 3Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei, 4School of Medicine, National Yang-Ming University, Taipei, 5Department of Internal Medicine, National Cheng Kung University Medical College and Hospital, Tainan, 6Infectious Disease and Signaling Research Center, National Cheng Kung University, Tainan, 7Department of Agronomy, National Chiayi University, Chia-Yi, 8Department of Nursing, Dalin
The effect of ingesting charcoal-broiled beefburgers (CBB) on the liver functions and nonenzymatic antioxidant levels in human blood was examined in twenty-nine healthy individually males (mean age 21.65 ± 1.32 years, range 20.32 - 22.42 years), non-smokers and had no occupational exposure to PAHs, who consumed two charcoal grilled beefburger per day (mean weight 70 gmper each) at lunch time over 28 consecutive days. The mean daily intake of PAH during the consumption period was 3431 ng and the mean daily intake of PAH per kg body wt/day was 46 ng. Blood samples were collected from each subject 7, 14, 21 and 28 days before, during, and after the beefburgers consumption period. glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvate transaminase (GPT) and alkaline phosphatase (ALP) were significantly higher in serum of subjects during CBB consumption period compared with those of before CBB consumption ones. All of the enzyme activities still increased upper the baseline levels, before CBB
1AMS: X-ray crystallographic study of pyridoxamine 5-phosphate-type aspartate aminotransferases from Escherichia coli in three forms.
Abstract The alcoholic extract of fruits of the plant Luffa acutangula previously reported for is hepatoprotective activity was fractionated into three parts to chemically identify the most potent bioactive fraction. The hepatoprotective potential of the fraction prepared from extract was studied in vivo in rats as well as ex vivo in isolated hepatocytes against paracetamol induced hepatotoxicity. The hepatoprotective activity was determined on the basis of their effects on parameters like direct bilirubin, serum aspartate transaminase, serum alanine transaminase and alakaline phasphatase. Ethyl acetate fraction (EAF) of alcoholic extract 100 mg/kg, p.o.) exhibited maximum hepatoprotective activity and is the active fraction for hepatoprotective activity of L. acutangula fruit.. ...
Viruses regulate host metabolic networks to improve their survival. The molecules that are responsive to viral infection and regulate such metabolic changes are hardly known, but are essential for understanding viral infection. Here we identify a long noncoding RNA (lncRNA) that is induced by multiple viruses, but not by type I interferon (IFN-I), and facilitates viral replication in mouse and human cells. In vivo deficiency of lncRNA-ACOD1 (a lncRNA identified by its nearest coding gene Acod1, aconitate decarboxylase 1) significantly attenuates viral infection through IFN-I-IRF3 (interferon regulatory factor 3)-independent pathways. Cytoplasmic lncRNA-ACOD1 directly binds the metabolic enzyme glutamic-oxaloacetic transaminase (GOT2) near the substrate niche, enhancing its catalytic activity. Recombinant GOT2 protein and its metabolites could rescue viral replication upon lncRNA-ACOD1 deficiency and increase lethality. This work reveals a feedback mechanism of virus-induced lncRNA-mediated ...
The liver has transaminases to synthesize and break down amino acids and to convert energy storage molecules. The concentrations of these transaminases in the serum (the non-cellular portion of blood) are normally low. However, if the liver is damaged, the liver cell (hepatocyte) membrane becomes more permeable and some of the enzymes leak out into the blood circulation. The two transaminases commonly measured are alanine transaminase (ALT) and aspartate transaminase (AST).[1] These levels previously were called serum glutamate-pyruvate transaminase (SGPT) and serum glutamate-oxaloacetate transaminase (SGOT). Elevated levels are sensitive for liver injury, meaning that they are likely to be present if there is injury. However, they may also be elevated in other conditions such as thyroid disorders, celiac disease, and muscle disorders.[4] ALT is usually found only in the liver. AST is most commonly found in the liver, but also in significant amounts in heart (cardiac) and skeletal muscle. ...
Saputri, F. C., Astari, C., Janatry, D. A., ., A., & Kusmana, D. (2018). HEPATOPROTECTIVE EFFECT OF BELLAMYA JAVANICA: ASPARTATE TRANSAMINASE, ALANINE AMINOTRANSFERASE, AND ALKALINE PHOSPHATASE ACTIVITY, AND LIVER HISTOPATHOLOGY IN MICE INDUCED WITH CARBON TETRACHLORIDE. International Journal of Applied Pharmaceutics, 10(1), 203-207. https://doi.org/10.22159/ijap.2018. ...
The mission of IJRHS (An international, double-blind peer-reviewed Quarterly Journal) is to provide its readers with up-to-date information relevant to Health Sciences. The journal policy is to publish work deemed by peer reviewers to be a coherent and sound addition to scientific knowledge and to put less emphasis on interest levels, provided that the research constitutes a useful contribution to the field. The Journal publishes original research papers, review articles, case studies, reports and short communications in all aspects of Health Sciences.. ...
On starch-gel or polyacrylamide-gel electrophoresis of human serum, a supernumerary zone of aspartate aminotransferase activity may be demonstrated, migrating with the slow α2 protein zone. This appearance is due only to cationic aspartate aminotransferase, bound by α2-macroglobulin. The binding is strongly potentiated by dilute borate buffers.. ...
This study aimed to figure out the underlying mechanism of miR-142-5p in the non-alcoholic steatohepatitis (NASH). Bioinformatics, luciferase assay and Western blot were performed. The NASH mouse model was established through feeding a high fat diet (HFD). Relative expressions of miR-142-5p, thymic stromal lymphopoietin (TSLP), inflammatory factors were detected by qRT-PCR. The injury level of liver was assessed via measurement of serum alanine aminotransferase (ALT) and serum aspartate aminotransferase (AST). H&E staining and Masson’s trichrome staining examine the liver fatty degeneration and fibrosis. MiR-142-5p and TSLP were differentially expressed and JAK-STAT signaling pathway was activated in the NASH group. Luciferase assay identified that TSLP was the downstream target of miR-142-5p. Through overexpression of miR-142-5p, ALT and AST in serum were inhibited, pro-inflammatory factors, liver fatty degeneration and fibrosis in liver tissues were decreased, while anti
Results: Results obtained showed that serum alkaline phosphatase (ALP), alanine amino transferase (ALT) and aspartate amino transferase (AST) activities in the infected-untreated control group were significantly different (P,0.05) and higher as compared to infected treated and normal control. Similarly, the two minerals significantly decreased (P,0.05) the levels of Urea and Creatinine in the infected treated when compared to the infected untreated rats, with the exception of creatinine value of the post-infected treated with selenium where there was no significant (P,0.05) decrease. On the other hand, a significant (P,0.05) increase in serum levels of total Protein, albumin and total bilirubin and no significant (P,0.05) difference in direct bilirubin was recorded in infected treated group compared to infected untreated and normal control. ...
Body mass index, fasting insulin, and aspartate aminotransferase or alanine aminotransferase ratio also differed.. The team found that quantitative insulin sensitivity check index, and hyaluronic acid differed between the 2 groups.. Hyaluronic acid composite index for distinguishing steatosis from nonalcoholic steatohepatitis was calculated.. The team calculated this index by summing up risk factors that included age 50 years or more, and female gender.. Other risk factors assessed were aspartate aminotransferase 45 IU/l or more, and body mass index 30 mg/kg2 or more.. The index was also calculated by an aspartate aminotransferase/alanine aminotransferase ratio of 0.8 or more, and hyaluronic acid of 55 mcg/l or more.. The team determined the accuracy of the index by receiver operating characteristic analysis, and found this to be 0.76.. The presence of 3 or more risk factors had a sensitivity of 74% and a specificity of 66%.. In addition, the researchers noted that a hyaluronic acid cutoff of 45 ...
Aspartate aminotransferase (AST) catalyzes the transfer of the amino group from L-aspartate to α-Ketoglutarate to yield oxalacetate and L-glutamate. The oxalacetate undergoes reduction with simultaneous oxidation of NADH to NAD in the malate dehydrogenase (MDH) catalyzed indicator reaction. The resulting rate of decrease in absorbance at 340nm is directly proportional to the AST activity. Lactate dehydrogenase (LDH) is added to prevent interference from endogenous pyruvate which is normally present in serum.. ...
Disease Markers is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies related to the identification of disease markers, the elucidation of their role and mechanism, as well as their application in the prognosis, diagnosis and treatment of diseases.
Alanine aminotransferase (ALT) is an enzyme found mostly in liver and kidney cells. ALT is released into the blood when the liver is damaged. The ALT blood test is often ordered in conjunction with aspartate aminotransferase (AST) or as part of a liver panel to screen for and/or help diagnose liver disease.
Prof. Vivian I. Teichberg of the Institutes Neurobiology Department first demonstrated a possible way around these problems in 2003. Glutamate - a short-lived neurotransmitter - is normally all but absent in brain fluids. After a stroke or injury, however, the glutamate levels in brain fluid become a flood that over-excites the cells in its path and kills them. Instead of attempting to get drugs into the brain, Teichberg had the idea that one might be able to transport glutamate from the brain to the blood using the tiny pumps, or transporters, on the capillaries that work on differences in glutamate concentration between the two sides. Decreasing glutamate levels in blood would create a stronger impetus to pump the substance out of the brain. He thought that a naturally-occurring enzyme called glutamate-oxaloacetate transaminase (GOT, for short) could scavenge blood glutamate, significantly lowering its levels. By 2007, Teichberg and his colleagues had provided clear evidence of the very ...
Citrate accumulation and secretion are physiological functions of the normal prostate gland in most species. Prolactin (PRL) stimulates citrate accumulation in the rat lateral prostate (LP) and pig prostate by increasing the expression of mitochondrial aspartate aminotransferase (mAAT), the enzyme that catalyzes aspartate transamination. In the present studies, we established the role of PRL and the phorbol ester 12-O-tetra-decanoylphorbol 13-acetate (TPA) in the regulation of mAAT in LNCaP and PC-3 cells. We isolated total RNA from the cells and hybridize it with a mAAT cDNA. Next, we assayed protein kinase C (PKC) activity and expression in PRL and TPA treated prostate cells. We, then determined the effect of PRL and TPA on gene transcription using a mAAT-chloramphenicol acetyltransferase (CAT) reporter gene construct, transiently transfected into PC-3 cells. A fragment of the 5{dollar}\sp\prime{dollar} gene region containing putative TPA response elements (TRE) was cloned into the ...
1. Cytoplasmic aspartate aminotransferase was isolated and purified from human heart with a final specific activity of 236 units/mg of protein.. 2. Three distinct peaks of aspartate aminotransferase activity were detected by isoelectric focusing with isoelectric points of 5.46, 5.60 and 5.71. Two minor subforms were also noted as shoulder patterns with pI 5.2 and 5.8.. 3. These electrophoretic characteristics are similar to previous findings of multiple molecular forms detected in human liver and erythrocytes.. ...
TY - JOUR. T1 - Use of protease sensitivity to probe the conformations of newly synthesised mutant forms of mitochondrial aspartate aminotransferase. AU - Azzariti, Amalia. AU - Giannattasio, Sergio. AU - Doonan, Shawn. AU - Merafina, Riccardo S.. AU - Marra, Ersilia. AU - Quagliariello, Ernesto. PY - 1995. Y1 - 1995. N2 - Sensitivity to digestion with pronase has been used to show that the precursor form of mitochondrial aspartate aminotransferase, the form lacking the N-terminal presequence, that with a deletion of the first 9 residues and mutants of the mature enzyme in which residue Cys-166 is mutated to alanine or serine, all retain unfolded conformations after synthesis in a reticulocyte lysate. In the presence of lysed mitochondria the various forms of mitochondrial aspartate aminotransferase retained their susceptibilities to pronase in a way that mirrored the efficiencies with which they are imported into intact mitochondria. The results are interpreted as showing that the presequence ...
Book; Format: print ; Literary form: not fiction Publisher: 1991Dissertation note: Fascioliasis is one of the major economically important diseases affecting a mass population of sheep and goats in Pakistan. The present study was designed to find out the prevalence of fascioliasis in N.W.F.P., and the serum bilirubin and serum glutamic oxaloacetic transaminase activities in sheep and goats before and after treatment with Nitroxynil. In this study out of 240 sheep and 360 goats forty sheep (16.667.) and forty goats (11.117.) were found positive for fascioliasis, by applying direct smear and zinc sulphate floatation technique. The eggs per gram of faeces were done by Mc Master eggs counting technique. Serum samples of forty sheep and forty goats positive for fascioliasis and twenty sheep and twenty goats negative for the same were examined for bilirubin and SGOT activities before & after medication with Nitroxynil with a dose rate of 1 ml/20 kg body weight, sub cutaneously. The drug was highly ...
The effect of Bacillus additive on immunity and antioxidant activities of grass carp (Ctenopharyngodon idellus) was studied in this experiment. Animals of the control group were fed the basal diet and those of treatment group were fed the basal diet augmented with Bacillus additive at a dose of 105 Colony Forming Units per gram (CFU g-1) feed. Data showed that serum immunity parameters including IgM, complement C3 and Alkaline Phosphatase (AKP) significantly increased (p,0.05) by 30.77, 34.62 and 107.79% in the treatment group. However, there was no significant difference in serum total protein, globulin, albumin, A/G ratio, Lysozyme (LZM), Glutamic Oxaloacetic Transaminase (GOT) and Glutamic Pyruvic Transaminase (GPT) activities. Serum antioxidant parameters such as serum Glutathione (GSH), Total Antioxidant Capacity (T-AOC), Superoxide Dismutase (SOD) and Glutathione Peroxidase (GSH-Px) activities significantly (p,0.05) enhanced by 32.76, 41.75, 20.06 and 11.30%, respectively and ...
The effect of Bacillus additive on immunity and antioxidant activities of grass carp (Ctenopharyngodon idellus) was studied in this experiment. Animals of the control group were fed the basal diet and those of treatment group were fed the basal diet augmented with Bacillus additive at a dose of 105 Colony Forming Units per gram (CFU g-1) feed. Data showed that serum immunity parameters including IgM, complement C3 and Alkaline Phosphatase (AKP) significantly increased (p,0.05) by 30.77, 34.62 and 107.79% in the treatment group. However, there was no significant difference in serum total protein, globulin, albumin, A/G ratio, Lysozyme (LZM), Glutamic Oxaloacetic Transaminase (GOT) and Glutamic Pyruvic Transaminase (GPT) activities. Serum antioxidant parameters such as serum Glutathione (GSH), Total Antioxidant Capacity (T-AOC), Superoxide Dismutase (SOD) and Glutathione Peroxidase (GSH-Px) activities significantly (p,0.05) enhanced by 32.76, 41.75, 20.06 and 11.30%, respectively and ...
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There is a very little information about the protective effect of lycopene (LYC) against hepatic ischemia-reperfusion injury. The present study was designed to examine the possible protective effect of the strong antioxidant and anti-inflammatory agent, LYC, on hepatic ischemia/reperfusion injury. For this purpose, rats were subjected to 45 min of hepatic ischemia followed by 60 min of reperfusion period. LYC at the doses of 2.5 and 5 mg/kg body weight (bw) were injected intraperitoneally, 60 min prior to ischemia. Upon sacrification, hepatic tissue samples were used for the measurement of catalase (CAT) activity and malondialdehyde (MDA) levels. Also, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were assayed in serum samples. As a result of the use of LYC at the doses of 2.5 and 5 mg/kg bw; while improvements of the ALT, AST, LDH and MDA values were partial and dose-dependent, the improvement of CAT activity was total and dose-independent (p , ...
ABSTRACT:BACKGROUND:Non Steroidal Anti-Inflammatory drugs (NSAIDs) are used to treat musculoskeletal disorders, inflammation and to control pain. Virtually all (NSAIDs) are capable of producing liver injury ranging from mild reversible elevation of liver enzymes to severe hepatic failure.OBJECTIVE:To estimate the hepatic risk associated with the use of some NSAIDs. SUBJECT AND METHOD:80 osteoarthritic patients were on diclofenac acid (voltarin) tablets 75 mg, 60 of them were female and 20 were male, laboratory estimation of serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST), serum alkaline phosphatase activity and total serum bilirubin (TSB) were done. For comparison age and sex matched 96 apparently healthy persons serve as controls.RESULTS:27 (33.75 %) of the diclofenac treated patients had some impairment of liver function tests, 66.6 % of the liver injury found in patients aged more than 50 years and 88.8 % had occurred in females. hepatocelluar injury characterize ...
The impact of adolescent nonalcoholic fatty liver disease (NAFLD) on health, independent of fat mass, is unclear.The objective of the study was to determine the independent (of total body fat) association of ultrasound scan (USS)-determined NAFLD with liver fibrosis, insulin resistance, and dyslipidemia among healthy adolescents.This was a cross-sectional analysis in participants from a UK birth cohort.One thousand eight hundred seventy-four (1059 female) individuals of a mean age of 17.9 years participated in the study.USS assessed liver stiffness (shear velocity, an indicator of fibrosis) and volume, fasting glucose, insulin, triglycerides, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, alanine amino transferase, aspartate amino transferase, γ-glutamyltransferase, and haptoglobin.The prevalence of NAFLD was 2.5% [95% confidence interval (CI) 1.8-3.3] and was the same in females and males. Dual-energy X-ray absorptiometry determined total body fat mass was strongly ...
BIOLABO, AST/TGO - ALT/TGP colorimetric Method, End point colorimetric method. Reagent for quantitative determination of Alanine amino transferase (ALT) and Aspartate amino transferase (AST) activities in human serum and plasma. Reading : End Point at 505 nm.
The hepatocurative effect of aqueous root extract of Calitropis Procera on CCl4 induced hepatotoxicity in rabbits was studied in groups of rabbit and the levels of liver enzymes; aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP). Serum concentrations of total bilirubin and direct bilirubin were found to be significantly (p,0.05) higher in group induced with hepatotoxicity, with significantly (p,0.05) lower total protein and urea compared to the control group. These findings confirmed induction of hepatotoxicity. Three different dosages were orally administred (1.0g/kg, 2.5g/kg and 3.6 g/kg) twice daily for 9 days. The C. Procera root extract was found to significantly (p,0.05) reduce the serum levels of AST, ALT, and ALP, serum concentration of total bilirubin in rats with hepatotoxicity compared to the the control group and significantly (p,0.05) incerased the serum concentration of total protein and urea. These indicates the possible ...
Patients and methods: In this cross sectional study conducted at diabetic and endocrine centre in A-Sadr medical city in Najaf, from February 2014 to March 2015 , a total of 120 type 2 diabetic patients were included (Male 39 and Female 81). Several different factors were studied like age, sex, type of treatment, body mass index and control of diabetes by measuring glycated hemoglobin. Liver transaminases (alanine aminotransferase and aspartate aminotransferase) was done to all those patients and the results are compared with these different factors that mention above. Ultrasonography was done to all the patients searching for any signs of non-alcoholic fatty liver disease like increase in liver size or echogenicity and compared with these factors ...
Reference: Ilin V.S., Kovner F.Ia., Hexokinase, aspartate and alanine aminotransferase activity in transplanted rat sarcomas, Voprosy meditsinskoi khimii, 1971, vol: 17(6), 644-649 ...
109/L) if secondary to bone marrow involvement by disease.. 3. Serum aspartate transaminase (AST/SGOT) or alanine transaminase (ALT/SGPT) , 3.0 x upper limit of normal (ULN). 4. Serum bilirubin , 1.5 x ULN unless due to Gilberts syndrome. 5. Calculated creatinine clearance of ≥ 60 ml/min. Exclusion Criteria: 1. Presence of any significant medical condition, laboratory abnormality, or psychiatric illness that would prevent the subject from participating in the study. 2. Prior allogeneic stem cell transplant (SCT)/bone marrow transplant within 12 months of signing the ICD. Subjects who received allogeneic SCT ≥ 12 months before signing the ICD may be eligible provided there is no ongoing graft-versus-host disease and no ongoing immune suppression therapy. 3. Subject has received prior CAR-T or other T-cell targeting treatment (approved or investigational) ≤ 4 weeks prior to starting CC-99282. 4. Subject has received prior therapy with CRBN-modulating drug (eg, lenalidomide, ...
This page includes the following topics and synonyms: Alanine Aminotransferase, Serum Glutamic Pyruvic Transaminase, Glutamic Pyruvate Transaminase, Serum ALT, ALT, SGPT, Aminotransferase.
This study was performed to investigate the effect of feeding garcinia cambogia extract(HCA) and L-carnitine and exercise on body weight and lipid metabolism in rats. Forty-eight male rats(Charles River CD(SD) IGS) of eight weeks and weighing 323.5±2.4g were raised for two months with high fat diet(40% fat as calorie) to induce obesity. After induction of obesity, rats weighing 552.8±5.8g were blocked into eight groups according to body weight and raised for six weeks with diet containing HCA and L-carnitine and swimming. Animals were given diet and water ad libitum. Food intake and body weight gain were recorded for experimental period and perirenal fat pad, epididymal fat pad and brown adipose tissue were weighed. Plasma aspartate aminotransferase(AST) and alanine aminotransferase (ALT) activities were measured to examine the safety of experimental compound in rats and plasma total protein and albumin levels were analyzed to determine the protein nutritional state of rats. And in order to ...
FDAs review of adverse event reports supports a relationship between the use of various body-building products suspected to contain steroids and serious liver injury requiring hospitalization. A majority of the cases involved elevated liver enzymes (i.e., alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (AlkP)), which are indicators of liver injury. In some cases, consumers were taking multiple body-building products, which precludes the ability to limit the findings to a specific product or ingredient and raises the concern that the harmful effects may be caused by an interaction between the two products.. Drug-induced liver injury (DILI) is a known possible harmful effect of using anabolic steroid-containing products. In addition, anabolic steroids (or AAS) may cause other serious adverse effects such as abnormal fat and cholesterol in the blood, mood disorders, androgenic effects (acnes, baldness, excessive hair growth in females), gonadal ...
A group of blood tests used to detect, evaluate, and monitor liver disease or damage. A liver function test measures enzymes, proteins, and other substances that are produced or excreted by the liver, such as alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), bilirubin, and albumin.
The assessment of liver fibrosis in ESRD patients with chronic viral hepatitis is considered essential for indication of therapy, transplantation and prognosis.[17,18,19] It is also important to rule out liver cirrhosis, which if present, constitutes a contraindication for lone kidney transplantation because of poor post-transplant survival. Liver biopsy is the gold standard method for staging and grading of liver disease, but it is an invasive procedure in which the frequency of pain at biopsy site is reportedly 20%, while the incidence of mortality and other serious complications have been reported to be 0.01% and 0.3-0.57% respectively.[20-21] On the other hand, liver biopsy represents only 1/50,000 portion of the entire liver, due to which error of sampling is a likely possibility. To overcome this limitation, it is essential to use a thick needle (18-G) and collect 2 or more samples of sufficient length for correct assessment of fibrosis. Achieving this goal is difficult because of ...
Introduction: Glutathione (GSH) protects the tissue and cell from oxidative injury. Objectives: In the current study, we investigated the possible effects of GSH on liver markers, oxidative stress and inflammatory indices in rat with renal ischemia reperfusion (RIR) injury. Materials and Methods: Twenty-four adult male Wistar rats were divided into 3 groups (n=8). Group I (the control group), group II (the RIR group) received saline (0.25 mL/d, intraperitoneally; i.p.), group III as the RIR group that received GSH (100 mg/kg/d, i.p.). The treatment with saline or GSH began daily 14 days before RIR induction. RIR was induced by clamping renal pedicles for 45 minutes and 24 hours of reperfusion. Results: RIR significantly increased the serum level of nitric oxide (NO), the serum activities of aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), the serum and renal levels of malondialdehyde (MDA), and the serum activity of myeloperoxidase (MPO). However, RIR
လူအေတာ္မ်ားမ်ား အသဲအဆီဖံုးေရာဂါ ရွိေနေပမဲ့ လူေကာင္းလို ေနၾကေသးတယ္။ စမ္းသပ္တဲ့အခါ အသဲ ၾကီးေနတာ ေတြ႔ရႏိုင္တယ္။ အသဲလုပ္ငန္းကိုသိဘို႔ ေသြးစစ္ရင္ Alanine transaminase (ALT) နဲ႔ Aspartate aminotransferase (AST) ေခၚတဲ့ (အင္ဇိုင္းမ္) ေတြ မ်ားေနမယ္။ (ALT) နဲ႔ (AST) (အင္ဇိုင္းမ္) ေတြ မ်ားေနတာက အသဲေရာဂါ ရမွသာ ျဖစ္တာ မဟုတ္ပါ။ ၾကြက္သားေရာဂါေၾကာင့္လဲ ရေစတယ္။ CT scan နဲ႔ MRI လုပ္ရင္ ပိုသိႏိုင္တယ္။ Liver biopsy အသဲက အသားစ ထုတ္ယူစစ္ေဆးမွ ...
Diagnostic study: the easy liver fibrosis test (eLIFT), an at-a-glance sum of points attributed to age, gender, gamma-glutamyl transferase, aspartate aminotransferase (AST), platelets and prothrombin time, was developed for the diagnosis of advanced fibrosis. In the validation set, eLIFT and fibrosis-4 (FIB4) had the same sensitivity (78.0% vs. 76.6%, p=0.470) but eLIFT gave fewer false positive results, especially in patients ≥60years old (53.8% vs. 82.0%, p,0.001), and was thus more suitable as screening test. FibroMeter with vibration controlled transient elastography (VCTE) was the most accurate among the eight fibrosis tests evaluated. The sensitivity of the eLIFT-FMVCTE algorithm (first-line eLIFT, second-line FibroMeterVCTE) was 76.1% for advanced fibrosis and 92.1% for cirrhosis. Prognostic study: patients diagnosed as having no/mild fibrosis by the algorithm had excellent liver-related prognosis with thus no need for referral to a hepatologist ...
Lupinus species and their derivatives are good candidates to be used as hypoglycaemic agents. The aim of this study was to evaluate the effects of supplementation with Lupinus albus dry extract in type 2 diabetic patients. The study included 47 adult patients (21 men and 26 women) with type 2 diabetes. While consuming their usual medication, patients received a daily dose of 400 mg of Lupinus albus dry extract during 12 weeks. Fasting and post meal test glucose and insulin were measured at baseline and after 2 and 12 weeks. Plasma lipids, Alanine and Aspartate aminotransferases activities and glycated haemoglobin were assessed at baseline and at the end of the supplementation period. Compared to baseline values, fasting and postprandial plasma glucose levels were decreased at 2 and 12 weeks. Blood glucose area under the curve significantly decreased after 2 weeks (p|0.01) and 12 weeks (p|0.05) of lupine administration. Fasting insulin concentrations (10.3±5.34 vs. 11.9±6.58; p|0.05) and homeostasis
Alanine aminotransferase (ALT), a sensitive indicator of liver cell injury, has been used to identify patients with liver disease for almost 50 years (1). This cytosolic enzyme, which is found in many organs, catalyzes the transfer of the α-amino group from alanine to α-ketoglutaric acid (2). Alanine aminotransferase levels are particularly high in the liver. For detection of liver diseases, ALT is thought to be a more specific indicator than aspartate aminotransferase, an enzyme found in cytosol and mitochondria (3). Serum levels of ALT normally are low-167 to 667 nkat/L (10 to 40 U/L) in most laboratories; however, normal values may vary greatly among laboratories. Elevated serum ALT levels help identify many types of liver diseases in patients and were widely used in the 1980s to screen blood donors for non-A, non-B hepatitis. Any type of liver cell injury can modestly increase ALT levels. Values up to 5000 nkat/L (300 U/L) are considered nonspecific. Marked elevations-that is, ALT levels ...
Endosulfan-induced systemic toxicity arises from oxidative stress and glutathione depletion. This study aims to investigate the possible protective effect of N-acetyl-L-cysteine (NAC) against endosulfan-induced toxicity in the liver and kidney tissue of rats. Wistar albino rats were separated into 4 groups and administered saline, NAC, endosulfan and endosulfan + NAC for 5 days. After euthanizing the animals, trunk blood was collected, followed by the removal of the kidney and liver for histological observation, the determination of glutathione, malondialdehyde levels, collagen content and myeloperoxidase activity. Lactate dehydrogenase (LDH) activity, blood urea nitrogen, creatinine, alanine aminotransferase and aspartate aminotransferase levels were measured in serum samples, while 8-OHdG, IL-1b, IL-6 and TNF-alpha were analyzed in plasma. Endosulfan provoked a significant decrease in tissue glutathione, along with a significant increase in malondialdehyde and collagen status, as well as ...
abbr. (in clinical biochemistry): ALT; EC 2.6.1.2; systematic name: l‐alanine:2‐oxoglutarate aminotransferase; other names: glutamic‐pyruvic transaminase; glutamic‐alanine transaminase. An enzyme that catalyses the reversible reaction:. l‐alanine + 2‐oxoglutarate = pyruvate + l‐glutamate.. [...] ...
A doctor will first perform a complete physical exam, specifically looking for rashes and muscle weakness. If JM is suspected, blood tests are the next step in making a diagnosis. Muscle enzymes are measured, including creatine kinase (CK), aldolase, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and alanine aminotransferase (ALT). If these lab tests are elevated, enzymes are leaking from inflamed or damaged muscles into the bloodstream. Antinuclear antibodies (ANA) are also measured to see if the childs body is producing antibodies against its own cells. Several myositis-specific autoantibodies have also been identified, and these can be tested as well. Other blood tests are sometimes available to check out immune activation and/or blood vessel damage. The next step in diagnosing JM is usually an MRI which can detect muscle inflammation and damage. If the MRI shows evidence of diseased muscles, a muscle biopsy may be performed to finalize the diagnosis. When the characteristic ...
HCV patients were divided into two groups over a 12 month period. Group A received Peg-IFN-a 2b plus ribavirin plus L-carnitine, and Group B received Peg-IFN-a and ribavirin. Red cell count, hemoglobin, white cell count, platelets, bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and viremia were tested in all subjects ...
Alcohol is thought to cause injury to the mitochondria which contains AST but not ALT. In addition, in chronic alcoholics, pyridoxine (vitamin B6) deficiency may reduce the synthesis of ALT more than AST because the former is more B6-dependent (1). AST/ALT ratio |1 may be more common in advanced alcohol liver disease (e.g. cirrhosis)…
Alanine aminotransferase measurements are used in the diagnosis and treatment of certain liver diseases (e.g. viral hepatitis and cirrhosis) and heart diseases. In viral hepatitis and other forms of liver disease associated with hepatic necrosis, serum ALT levels are elevated even before clinical signs and symptoms of the disease ...
SGPT/Serum Glutamic Pyruvic Transaminase (Alanine Transaminase /ALT) adalah enzim yang ditemukan terutama di sel-sel hati dan ginjal, dan dalam jumlah yang lebih kecil ditemukan di jantung dan otot ...
Everything you need to know about Alanine Aminotransferase (ALT) and find out which of our blood tests check Alanine Aminotransferase levels.
The primary role of Tyr225 in the aspartate aminotransferase mechanism is to provide a hydrogen bond to stabilize the 3O- functionality of bound pyridoxal phosphate. The strength of this hydrogen bond is perturbed by replacement of Tyr225 with 3-fluoro-L-tyrosine (FlTyr) by in vitro transcription/translation. This mutant enzyme exhibits kcat/values that are near to those of wild type enzyme; however, the kcat/vs pH profile is much sharper with similar pKas of approximately 7.5 for both the ascending and descending limbs. The pKas are assigned to the endocyclic proton of the internal aldimine and to the bridging hydrogen bond, respectively. The pKas in the kcat vs pH profile of 7.2 and 8.7 are assigned to the epsilon-NH3+ of lysine 258 and to the endocyclic protons of the ketimine complex, respectively. Arginine 292 forms a salt bridge with the beta-COOH of the substrate, aspartate. An improvement on the earlier attempt to invert the substrate charge specificity via R292D mutation-induced ...
SGPT, or serum glutamic pyruvic transaminase, is an enzyme that enters the blood when the heart or liver are damaged, according to MedicineNet. When a condition like hepatitis C causes damage to the...
An experiment was conducted to detect the relationship between some serum enzyme activities, i.e. lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) enzymes and liver functions...
In this study aminotransferase inhibitors were used to determine the relative importance of different aminotransferases in providing nitrogen for de novo glutamate synthesis in the retina. Aminooxyacetate, which inhibits all aminotransferases, blocke
OBJECTIVES: Non-invasive fibrosis scores are widely used to identify/exclude advanced fibrosis in patients with non-alcoholic fatty liver disease (NAFLD). However, these scores were principally developed and validated in patients aged between 35 and 65 years of age. The objective of this study was to assess the effect of age on the performance of non-invasive fibrosis tests in NAFLD.. METHODS: Patients were recruited from European specialist hepatology clinics. The cohort was divided into five age-based groups: ≤35 (n=74), 36-45 (n=96), 46-55 (n=197), 56-64 (n=191), and ≥65 years (n=76), and the performance of the aspartate aminotransferase (AST)/alanine transaminase (ALT) ratio, fibrosis 4 (FIB-4), and NAFLD fibrosis score (NFS) for advanced fibrosis (stage F3-F4) for each group was assessed using liver biopsy as the standard.. RESULTS: Six hundred and thirty-four patients were included. The diagnostic accuracy of the AST/ALT ratio was lower than NFS and FIB-4 in all the age groups. The ...
OBJECTIVES: Non-invasive fibrosis scores are widely used to identify/exclude advanced fibrosis in patients with non-alcoholic fatty liver disease (NAFLD). However, these scores were principally developed and validated in patients aged between 35 and 65 years of age. The objective of this study was to assess the effect of age on the performance of non-invasive fibrosis tests in NAFLD.. METHODS: Patients were recruited from European specialist hepatology clinics. The cohort was divided into five age-based groups: ≤35 (n=74), 36-45 (n=96), 46-55 (n=197), 56-64 (n=191), and ≥65 years (n=76), and the performance of the aspartate aminotransferase (AST)/alanine transaminase (ALT) ratio, fibrosis 4 (FIB-4), and NAFLD fibrosis score (NFS) for advanced fibrosis (stage F3-F4) for each group was assessed using liver biopsy as the standard.. RESULTS: Six hundred and thirty-four patients were included. The diagnostic accuracy of the AST/ALT ratio was lower than NFS and FIB-4 in all the age groups. The ...
... aspartate aminotransferase 1)". Panteghini M (1990). "Aspartate aminotransferase isoenzymes". Clin. Biochem. 23 (4): 311-9. doi ... Totan A, Greabu M, Totan C, Spinu T (2006). "Salivary aspartate aminotransferase, alanine aminotransferase and alkaline ... Aspartate aminotransferase, cytoplasmic is an enzyme that in humans is encoded by the GOT1 gene. Glutamic-oxaloacetic ... Doyle JM, Schininà ME, Bossa F, Doonan S (1990). "The amino acid sequence of cytosolic aspartate aminotransferase from human ...
... inhibits aspartate aminotransferase, another PLP-dependent enzyme, which is an essential part of the malate ... Also, the inhibition of aspartate aminotransferase by aminooxyacetic acid has clinical implications for the treatment of breast ... Moreover, selective inhibition of aspartate aminotransferase with aminooxyacetic acid ameliorated experimental autoimmune ... "Targeting aspartate aminotransferase in breast cancer". Breast Cancer Research. 10 (5): R84. doi:10.1186/bcr2154. PMC 2614520. ...
Mavrides C, Orr W (1975). "Multispecific aspartate and aromatic amino acid aminotransferases in Escherichia coli". J. Biol. ... Other names in common use include aromatic amino acid aminotransferase, aromatic aminotransferase, and ArAT. This enzyme ... The systematic name of this enzyme class is aromatic-amino-acid:2-oxoglutarate aminotransferase. ...
Aspartate aminotransferase, mitochondrial is an enzyme that in humans is encoded by the GOT2 gene. Glutamic-oxaloacetic ... Guidetti P, Amori L, Sapko MT, Okuno E, Schwarcz R (Jul 2007). "Mitochondrial aspartate aminotransferase: a third kynurenate- ... Furuya E, Yoshida Y, Tagawa K (May 1979). "Interaction of mitochondrial aspartate aminotransferase with negatively charged ... "Entrez Gene: GOT2 glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)". Ford GC, Eichele G, ...
"Cytosolic aspartate aminotransferase inactivating-enzyme from Streptomyces violaceochromogenes". Agricultural and Biological ...
... increased levels of an enzyme called aspartate aminotransferase; headache; and infection in the lungs (pneumonia). Trilaciclib ...
"The primary structure of aspartate aminotransferase from pig heart muscle. Digestion with a proteinase having specificity for ...
The two common types of aminotransferases are Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST). Booth, ... Glutamate + oxaloacetate ↔ α-ketoglutarate + aspartate Transamination catalyzed by aminotransferase occurs in two stages. In ... The products usually are either alanine, aspartate or glutamate, since their corresponding alpha-keto acids are produced ... For the reaction to complete, aminotransferases require participation of aldehyde containing coenzyme, pyridoxal-5'-phosphate ( ...
It is also a member of the broader aspartate aminotransferase family. Like many other PLP-dependent enzymes, cystathionine ...
The alanine aminotransferase, aspartate aminotransferase and creatine kinase levels may be raised. The gamma- ...
Guidetti P, Amori L, Sapko MT, Okuno E, Schwarcz R (July 2007). "Mitochondrial aspartate aminotransferase: a third kynurenate- ... kynurenine aminotransferase, and L-kynurenine aminotransferase. This enzyme participates in tryptophan metabolism. It employs ... "Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II". Bioscience Reports ... "Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II". Bioscience Reports ...
The ratio of aspartate aminotransferase to alanine aminotransferase is usually 2 or more. In most cases, the liver enzymes do ... Sorbi D, Boynton J, Lindor KD (1999). "The ratio of aspartate aminotransferase to alanine aminotransferase: potential value in ... and aminotransferases, and onset of jaundice within the last 8 weeks. ...
For example, aspartate aminotransferase converts glutamate and oxaloacetate to alpha-ketoglutarate and aspartate. Other ... Thus for aspartate or glutamate with negative side chains, pI = 1/2(pKa1 + pKa(R)), where pKa(R) is the side chain pKa. ... Aspartate, glycine, and glutamine are precursors of nucleotides. However, not all of the functions of other abundant ... Cysteine also has potentially negative side chain with pKa(R) = 8.14, so pI should be calculated as for aspartate and glutamate ...
... may cause severe elevations in serum aminotransferases, alanine transaminase (ALT) and aspartate ...
"Structural features of the precursor to mitochondrial aspartate aminotransferase responsible for binding to hsp70". The Journal ...
"Mechanism of action of aspartate aminotransferase proposed on the basis of its spatial structure". Journal of Molecular Biology ... These reactions are facilitated by transaminases in order to produce aspartate and asparagine from oxaloacetate. Transamination ...
"Mechanism of action of aspartate aminotransferase proposed on the basis of its spatial structure". Journal of Molecular Biology ... In ATCase such a transfer is written as carbamoyl phosphate + L-aspartate → {\displaystyle \rightarrow } L-carbamoyl aspartate ... Transamination, or the transfer of an amine (or NH2) group from an amino acid to a keto acid by an aminotransferase (also known ... This includes enzymes like transaminase (also known as "aminotransferase"), and a very small number of oximinotransferases and ...
Aspartate aminotransferase to platelet ratio index may be used when cost is an issue. Hepatitis B virus DNA remains in the body ... Carriers of the virus may have chronic hepatitis B, which would be reflected by elevated serum alanine aminotransferase (ALT) ... Chronically infected individuals with persistently elevated serum alanine aminotransferase, a marker of liver damage, and HBV ...
Hsu YC, Tai DI (2011). "Unusually high alanine aminotransferase to aspartate aminotransferase ratio in a patient with ...
Here, the OAA produced by PEPC is transaminated by aspartate aminotransferase to aspartate (ASP) which is the metabolite ... In this variant the OAA produced by aspartate aminotransferase in the bundle sheath is decarboxylated to PEP by PEPC. The fate ... The first experiments indicating that some plants do not use C3 carbon fixation but instead produce malate and aspartate in the ... either malate or aspartate) in the mesophyll. The organic acid is produced then diffuses through plasmodesmata into the bundle ...
"Severe preeclampsia with fulminant and extreme elevation of aspartate aminotransferase and lactate dehydrogenase levels: high ... Elevated serum levels of certain proteins, in particular, LDH, alanine transaminase (ALT) and aspartate transaminase (AST), are ... an aspartate transaminase greater than 70 U/l, and platelets less than 100x109/l. If not all the criteria are present, the ...
The primary structure of aspartate aminotransferase from pig heart muscle. Digestion with a proteinase having specificity for ...
... function studies provide evidence of mild hepatocellular damage in patients with mild elevations of aspartate aminotransferase ... AST) and alanine aminotransferase (ALT) levels.[citation needed]Plasma amino-acid analysis shows plasma alanine levels as in ...
In people with alcoholic hepatitis, the serum aspartate aminotransferase (AST) to alanine aminotransferase (ALT) ratio is ...
... gamma glutamyl transpeptidase and aspartate aminotransferase activities and their significance as diagnostic aids". Res. Vet. ...
Several studies in rats have shown an increase in serum alanine aminotransferase and aspartate aminotransferase activities ( ...
For example, liver enzymes such as aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are found to be at ...
... aspartate aminotransferase increased, colitis, hypertension, maculopapular rash. However, Ivosidenib was taken in conjunction ... In ivosidenib-treated patients, reported adverse effects have been febrile neutropenia, alanine aminotransferase increased, ...
ALP and GGT levels typically rise with one pattern while aspartate aminotransferase (AST) and alanine aminotransferase (ALT) ... Some bone and heart disorders can lead to an increase in ALP and the aminotransferases, so the first step in differentiating ... The typical liver panel includes blood levels of enzymes found primarily from the liver, such as the aminotransferases (ALT, ...
... elevated levels of the liver enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST); and abnormalities in ...
The systematic name of this enzyme class is L-alanine:3-oxopropanoate aminotransferase. Other names in common use include beta- ... This enzyme participates in 4 metabolic pathways: alanine and aspartate metabolism, valine, leucine and isoleucine degradation ... Stinson RA, Spencer MS (1969). "Beta alanine aminotransferase (s) from a plant source". Biochem. Biophys. Res. Commun. 34 (1): ... alanine-pyruvate aminotransferase, and beta-alanine-alpha-alanine transaminase. ...
... the activities of aspartate aminotransferase linearly increased in plasma and decreased plasma insulin content without decrease ...
... three biochemical tests most widely used are the activation coefficient for the erythrocyte enzyme aspartate aminotransferase, ...
Aspartate + α-ketoglutarate ⇌ oxaloacetate + glutamate. Both pyruvate and oxaloacetate are key components of cellular ... "Compensation for D-glutamate auxotrophy of Escherichia coli WM335 by D-amino acid aminotransferase gene and regulation of murI ...
Branched-chain amino acid aminotransferase. *Branched-chain alpha-keto acid dehydrogenase complex ... Aspartic acid (aspartate). *DIDS. *Direct blue 71. *Erythro-4-methyl-L-glutamic acid ...
... β-aspartate-semialdehyde → Homoserine + lysine Aspartic Acid -OOC-CH2- Oxaloacetate → Aspartic Acid (aminotransferase) ... Aspartate → β-aspartate-semialdehyde → Homoserine → Threonine Cysteine HS-CH2- Serine → Cystathionine → α-ketobutyrate → ... α-ketoglutarate → Glutamic Acid (aminotransferase) ‡Shown at physiological conditions. *Complexes that are italicized are ... 3-phosphoglycerate → 3-phosphohydroxypyruvate (3-phosphoglycerate dehydrogenase) → 3-phosphoserine (aminotransferase) → Serine ...
LDH=Lactate dehydrogenase, Uric acid=Uric acid, AST=Aspartate aminotransferase, ALT=Alanine aminotransferase, Plt=Platelets, Cr ...
... belongs to the class V group of aspartate aminotransferase superfamily of structurally homologous pyridoxal 5'- ...
Ma MC, Huang HS, Chen YS, Lee SH (Nov 2008). "Mechanosensitive N-methyl-D-aspartate receptors contribute to sensory activation ... 3-phosphoserine phosphatase deficiency and Phosphoserine aminotransferase deficiency. These enzyme defects lead to severe ... soon after the discovery of D-aspartate. Had D amino acids been discovered in humans sooner, the glycine site on the NMDA ... "D-serine is an endogenous ligand for the glycine site of the N-methyl-D-aspartate receptor". Proceedings of the National ...
Results: A significant reduction in alanine aminotransferase (ALT) (64 ± 72 vs 46 ± 36, P=0.016) and aspartate aminotransferase ...
"Hazardous drinking is associated with elevated aspartate aminotransferase to platelet ratio index in an urban HIV clinical ... Increase of aminotransferases]". Clinique médicale, CHU de Rouen; Trivalle C, Chassagne P, Doucet J, Perol MB, Landrin I, ... "Coffee and caffeine consumption reduce the risk of elevated serum alanine aminotransferase activity in the United States". ... "Contribution of metabolic factors to alanine aminotransferase activity in persons with other causes of liver disease". ...
... aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma glutamyl transferase (GGT), alkaline phosphatase (ALP ...
Type II tyrosinemia is caused by a deficiency of the enzyme tyrosine aminotransferase (EC 2.6.1.5), encoded by the gene TAT. ... Tyrosine aminotransferase is the first in a series of five enzymes that converts tyrosine to smaller molecules, which are ... aspartate). *Argininemia. *Argininosuccinic aciduria. *Carbamoyl phosphate synthetase I deficiency. *Citrullinemia. *N- ...
Glutamine oxoglutarate aminotransferase cycle but costs the cell one ATP and one NADPH. The upside is that one CO2 is produced ...
The final step in the parallel pathway is conducted by amino transferase, which yields the final products of valine and ... Enzymes involved are branched chain aminotransferase and 3-methyl-2-oxobutanoate dehydrogenase. ... Aspartate. Group Homoserine. Group. & Lysine Glutamate. Group. & Proline Arginine Creatine. & Polyamines Ketogenic &. ... and aminotransferase - are necessary for the formation of leucine from 2-oxolsovalerate.[3] ...
... determined that the appropriate terminology for AST and ALT are aspartate aminotransferase and alanine aminotransferase. The ... Redirected from Alanine aminotransferase). Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called ... "Alanine aminotransferase: analyte monograph" (PDF). Association for Clinical Biochemistry and Laboratory Medicine. Retrieved 7 ... ALT (and all aminotransferases) require the coenzyme pyridoxal phosphate, which is converted into pyridoxamine in the first ...
Oxaloacetate/aspartate: lysine, asparagine, methionine, threonine, and isoleucine[edit]. The oxaloacetate/aspartate family of ... The enzyme involved in this reaction is an aminotransferase. α-ketoacid + glutamate ⇄ amino acid + α-ketoglutarate. Glutamate ... Aspartate[edit]. The biosynthesis of aspartate frequently involves the transamination of oxaloacetate. ... The formation of aspartate kinase (AK), which catalyzes the phosphorylation of aspartate and initiates its conversion into ...
Branched-chain amino acid aminotransferase. *Branched-chain alpha-keto acid dehydrogenase complex ... Glutamate aspartate transporter. *Glycerol-3-phosphate dehydrogenase. *ATP synthase. *Carnitine palmitoyltransferase II ...
These levels was not nearly as high for workers from the water cleanup crew, but the aspartate aminotransferase levels were ...
Aminotransferase, class V. *Amphibian antimicrobial peptides. *Androctonus australis hector insect toxin. *Androgen-binding ...
Branched-chain amino acid aminotransferase. *Branched-chain alpha-keto acid dehydrogenase complex ...
Branched-chain amino acid aminotransferase. *Branched-chain alpha-keto acid dehydrogenase complex ...
Brief elevations of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma-glutamyltransferase (GGT) ...
... diaminobutyrate aminotransferase, DABA aminotransferase, DAB aminotransferase, EctB, diaminibutyric acid aminotransferase, and ... L-aspartate 4-semialdehyde + L-glutamate Thus, the two substrates of this enzyme are L-2,4-diaminobutanoate and 2-oxoglutarate ... The systematic name of this enzyme class is L-2,4-diaminobutanoate:2-oxoglutarate 4-aminotransferase. Other names in common use ... include L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase, 2,4-diaminobutyrate 4-aminotransferase, ...
... prephenate aspartate aminotransferase, and L-aspartate:prephenate aminotransferase. De-Eknamkul W, Ellis BE (1988). " ... In enzymology, an aspartate-prephenate aminotransferase (EC 2.6.1.78) is an enzyme that catalyzes the chemical reaction L- ... The systematic name of this enzyme class is L-arogenate:oxaloacetate aminotransferase. Other names in common use include ... whereas its two products are prephenate and L-aspartate. This enzyme belongs to the family of transferases, specifically the ...
The aspartate aminotransferase (AST) blood test measures the level of the enzyme AST in the blood. ... Aspartate aminotransferase (AST, aspartate transaminase, SGOT) - serum. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and ... The aspartate aminotransferase (AST) blood test measures the level of the enzyme AST in the blood. ...
ASPARTATE AMINOTRANSFERASE. A. 401. Gallus gallus. Mutation(s): 0 Gene Names: GOT2. EC: 2.6.1.1 (PDB Primary Data), 2.6.1.7 ( ... Aspartate aminotransferase complexed with erythro-beta-hydroxyaspartate: crystallographic and spectroscopic identification of ... Structural Basis for Catalysis by Aspartate Aminotransferase. Jansonius, J.N., Vincent, M.G.. (1987) Biological ... The crystal structure of mitochondrial aspartate aminotransferase (mAAT) of chicken complexed with erythro-beta- ...
... ,ARUP Laboratories is a national reference laboratory and a worldwide leader in ... Aspartate Aminotransferase (AST) (GOT). 2. Aspartate Aminotransferase (AST) (GOT) IFCC WITH P5P. 3. Alanine Aminotransferase, ...
Aspartate aminotransferase may be measured as part of a liver function test. ... Aspartate aminotransferase may be measured as part of a liver function test. ... Aspartate Aminotransferase (AST) Aspartate Aminotransferase Speaker Synonym(s): Serum Glutamic-Oxaloacetic Transaminase ...
An aspartate aminotransferase (AST) blood test is often part of an initial screening for liver problems. ... An AST test measures the level of aspartate aminotransferase, also called AST or SGOT. AST is one of the enzymes that help the ...
Your doctor can order an AST test to check the level of aspartate aminotransferase in your blood. Abnormal results may be a ... Aminotransferase (AST) is an enzyme thats present in various parts of your body. ... What is aspartate aminotransferase?. Aminotransferase (AST) is an enzyme thats present in various tissues of your body. An ... An alanine aminotransferase (ALT) test measures the level of ALT in your blood. ALT is an enzyme made by cells in your liver. ...
tr,A0A3B5QAR0,A0A3B5QAR0_XIPMA Aspartate aminotransferase OS=Xiphophorus maculatus OX=8083 PE=4 SV=1 ... IPR004839 Aminotransferase_I/II. IPR000796 Asp_trans. IPR004838 NHTrfase_class1_PyrdxlP-BS. IPR015424 PyrdxlP-dep_Trfase. ... IPR004839 Aminotransferase_I/II. IPR000796 Asp_trans. IPR004838 NHTrfase_class1_PyrdxlP-BS. IPR015424 PyrdxlP-dep_Trfase. ...
An aspartate aminotransferase (AST) test measures the amount of this enzyme in the blood. AST is normally found in red blood ... Aspartate Aminotransferase (AST). Test Overview. An aspartate aminotransferase (AST) test measures the amount of this enzyme in ... The aspartate aminotransferase (AST) test is more effective than the alanine aminotransferase (ALT) test for detecting liver ... An aspartate aminotransferase (AST) test measures the amount of this enzyme in the blood. Results are usually available within ...
An aspartate aminotransferase (AST) test measures the amount of this enzyme in the blood. AST is normally found in red blood ... Aspartate Aminotransferase (AST) Test. Test Overview. An aspartate aminotransferase (AST) test measures the amount of this ... An aspartate aminotransferase (AST) test measures the amount of this enzyme in the blood. Results are usually available within ... The AST test may be done at the same time as a test for alanine aminotransferase, or ALT. The ratio of AST to ALT sometimes can ...
Stability of aspartate aminotransferase from Sulfolobus solfataricus.. Arnone MI1, Birolo L, Pascarella S, Cubellis MV, Bossa F ... Aspartate aminotransferase from Sulfolobus solfataricus (SsAspAT) is an extremely thermophilic and thermostable dimeric enzyme ... Therefore, in the case of aspartate aminotransferases, thermal stability does not correlate with the stability against chemical ... SsAspAT is less resistant to guanidinium chloride-induced denaturation than the cytosolic aspartate aminotransferase from pig ...
Aspartate aminotransferase complexed with erythro-beta-hydroxyaspartate: crystallographic and spectroscopic identification of ... ASPARTATE AMINOTRANSFERASE A 401 Gallus gallus EC#: 2.6.1.1 IUBMB 2.6.1.7 IUBMB Mutation: S45P Gene Name(s): GOT2 Gene View ...
References for Abcams Anti-Aspartate Aminotransferase antibody (ab104265). Please let us know if you have used this product in ...
Rabbit polyclonal Aspartate Aminotransferase antibody. Validated in WB and tested in Cow. Immunogen corresponding to ... Anti-Aspartate Aminotransferase antibody (ab232750) at 2 µg/ml + Recombinant cow Aspartate Aminotransferase protein.. Predicted ... Primary - Rabbit Anti-Aspartate Aminotransferase antibody (ab232750) WB Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718) ... All lanes : Anti-Aspartate Aminotransferase antibody (ab232750) at 2 µg/ml. Lane 1 : Cow brain lysate. Lane 2 : Cow heart ...
Aspartate Aminotransferase (AST). Test Overview. An aspartate aminotransferase (AST) test measures the amount of this enzyme in ... The aspartate aminotransferase (AST) test is more effective than the alanine aminotransferase (ALT) test for detecting liver ... An aspartate aminotransferase (AST) test measures the amount of this enzyme in the blood. Results are usually available within ... The AST test may be done at the same time as a test for alanine aminotransferase, or ALT. The ratio of AST to ALT sometimes can ...
The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte ... Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory ... L-aspartate:2-oxoglutarate aminotransferase activity Source: UniProtKB. *L-cysteine:2-oxoglutarate aminotransferase activity ... Aspartate aminotransferase, cytoplasmicAdd BLAST. 411. Amino acid modifications. Feature key. Position(s). DescriptionActions. ...
Browse our Aspartate Aminotransferase Antibody Pair catalog backed by our Guarantee+. ... Aspartate Aminotransferase Antibody Pairs available through Novus Biologicals. ... Aspartate Aminotransferase Antibody Pairs. We offer Aspartate Aminotransferase Antibody Pairs for use in common research ... Alternate Names for Aspartate Aminotransferase Antibody Pairs. Aspartate Aminotransferase antibody pair, GOT1 antibody pair, ...
BioVision Inc Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit: High-Throughput Suitable Assay for ... Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit: High-Throughput Suitable Assay for Measuring ALT/SGPT ... It catalyzes the reaction: Aspartate + ⍺-Ketoglutarate ⇌ Oxaloacetate + Glutamate Diagnostically, it is almost always measured ... in units/liter (U/L). In this Kit, an amino group is transferred of from aspartate to ⍺-ketoglutarate. The products of this ...
2.3 Global Aspartate Aminotransferase Test Market Overview. 2.4 COVID-19 Impact on Aspartate Aminotransferase Test Industry. ... 4.4.2 UK Aspartate Aminotransferase Test Market Size and Price Analysis 2015-2020. 4.4.3 France Aspartate Aminotransferase Test ... 4.5.3 GCC Aspartate Aminotransferase Test Market Size and Price Analysis 2015-2020. 4.6 Global Aspartate Aminotransferase Test ... 4.3.1 China Aspartate Aminotransferase Test Market Size and Price Analysis 2015-2020. 4.3.2 Japan Aspartate Aminotransferase ...
... aspartate aminotransferase (AST) levels in the gingival crevicular fluid (GCF) are utilized as a predictor of periodontal ... Aspartate aminotransferase Periodontitis Periodontal regeneration therapy Predictor This is a preview of subscription content, ... Shimada K, Mizuno T, Uchida T, Kato T, Ito K, Murai S. Relationship between levels of aspartate aminotransferase in gingival ... Shimada K, Mizuno T, Ohshio K, Kamaga M, Murai S, Ito K. Analysis of aspartate aminotransferase in gingival crevicular fluid ...
Aspartate Aminotransferase, Mitochondrial*Aspartate Aminotransferase, Mitochondrial. *Aminotransferase, Mitochondrial Aspartate ... Aspartate Aminotransferases [D08.811.913.477.700.225]. *Aspartate Aminotransferase, Mitochondrial [D08.811.913.477.700.225.500] ... "Aspartate Aminotransferase, Mitochondrial" by people in Harvard Catalyst Profiles by year, and whether "Aspartate ... "Aspartate Aminotransferase, Mitochondrial" is a descriptor in the National Library of Medicines controlled vocabulary ...
AACC.org // ... // Clinical Chemistry // Clinical Case Studies // Persistent Increase in Aspartate Aminotransferase in an ... Persistent Increase in Aspartate Aminotransferase in an Asymptomatic Patient. *Severe Hyponatremia with High Urine Sodium and ... Laboratory studies revealed an isolated increase in aspartate aminotransferase (AST)1 that prompted consultation with a ...
What is aspartate aminotransferase test? Meaning of aspartate aminotransferase test medical term. What does aspartate ... Looking for online definition of aspartate aminotransferase test in the Medical Dictionary? aspartate aminotransferase test ... aspartate aminotransferase test. Also found in: Dictionary, Encyclopedia.. Related to aspartate aminotransferase test: Creatine ... Aspartate Aminotransferase Test. Definition. The Aspartate aminotransferase test measures levels of AST, an enzyme released ...
... recovery and follow-up care for Aspartate aminotransferase (AST) blood test. ... Learn about Aspartate aminotransferase (AST) blood test, find a doctor, complications, outcomes, ... Aspartate aminotransferase (AST) blood test. Aspartate aminotransferase; Serum glutamic-oxaloacetic transaminase; SGOT ... Aspartate aminotransferase (AST, aspartate transaminase, SGOT) - serum. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and ...
Keywords: aspartate aminotransferase to platelet ratio index, chronic hepatitis C, hepatitis C virus, hepatocellular carcinoma ... Thirteen of them had high baseline aspartate aminotransferase to platelet ratio index (APRI) (ie, an APRI ,2.0). Multivariate ... Aspartate aminotransferase to platelet ratio index and sustained virologic response are associated with progression from ... Aspartate aminotransferase to platelet ratio index and sustained virologic response are associated with progression from ...
ASPARTATE AMINOTRANSFERASE INCREASED Causes, Patient Concerns and Latest Treatments and Tylenol Reports and Side Effects. ... Check out the latest treatments for ASPARTATE AMINOTRANSFERASE INCREASED. ➢ ASPARTATE AMINOTRANSFERASE INCREASED treatment ... ASPARTATE AMINOTRANSFERASE INCREASED Symptoms and Causes. We are sorry, but no medical information on this topic currently ... ASPARTATE AMINOTRANSFERASE INCREASED Clinical Trials and Studies. Treatments might be new drugs or new combinations of drugs, ...
Multiple Molecular Forms of Human Heart Cytoplasmic Aspartate Aminotransferase. F. Y. Leung, A. R. Henderson ... 1. Cytoplasmic aspartate aminotransferase was isolated and purified from human heart with a final specific activity of 236 ... Multiple Molecular Forms of Human Heart Cytoplasmic Aspartate Aminotransferase Message Subject (Your Name) has forwarded a page ... 2. Three distinct peaks of aspartate aminotransferase activity were detected by isoelectric focusing with isoelectric points of ...
INTERACTION OF ASPARTATE AMINOTRANSFERASE WITH AMINO ACIDS. P SCOTTO, V SCARDI. Biochemical Journal Jun 01, 1965, 95 (3) 657- ... INTERACTION OF ASPARTATE AMINOTRANSFERASE WITH AMINO ACIDS Message Subject (Your Name) has forwarded a page to you from ...
Aspartate aminotransferase (AST, aspartate transaminase, SGOT) - serum. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and ... The aspartate aminotransferase (AST) blood test measures the level of the enzyme AST in the blood. ...
... Antti, Henrik Umeå universitet, Teknisk- ... The interpretation of the data was enhanced by the use of two different cell-lines and supports aspartate aminotransferase as a ... Here two T-cell lines were treated with an inhibitor of aspartate aminotransferase and analyzed with untargeted GC-MS. ...
  • Other names in common use include prephenate transaminase (ambiguous), PAT (ambiguous), prephenate aspartate aminotransferase, and L-aspartate:prephenate aminotransferase. (wikipedia.org)
  • Aspartate aminotransferase (AST, aspartate transaminase, SGOT) - serum. (medlineplus.gov)
  • Aspartate aminotransferase (AST) is also known as serum glutamic oxaloacetic transaminase (SGOT). (thefreedictionary.com)
  • Lee Biosolutions is a producer and supplier of human liver enzyme Aspartate Aminotransferase/Glutamate Oxaloacetate Transaminase (AST/GOT) for research and clinical diagnostic assays for liver disease. (leebio.com)
  • Also known as Aspartate aminotransferase (AspAT) (Transaminase A). Aspartate aminotransferase (AspC) is a multifunctional enzyme that catalyzes the synthesis of aspartate, phenylalanine and other compounds via a transamination reaction. (mybiosource.com)
  • Also known as Aspartate aminotransferase 1 (Transaminase A). Important for the metabolism of amino acids and Krebs-cycle related organic acids. (mybiosource.com)
  • Also known as Aspartate aminotransferase 2 (Transaminase A) (Fragments). (mybiosource.com)
  • Also known as Aspartate aminotransferase 3, chloroplastic (Protein YELLOW-LEAF-SPECIFIC GENE 4) (Transaminase A). Encodes the chloroplastic isozyme of aspartate aminotransferase. (mybiosource.com)
  • Also known as Aspartate aminotransferase A (AspAT) (Transaminase A). Required for symbiotic nitrogen fixation. (mybiosource.com)
  • Also known as Aspartate aminotransferase B (AspAT) (Transaminase A). Involved in symbiotic nitrogen fixation. (mybiosource.com)
  • Also known as Aspartate aminotransferase, cytoplasmic (cAspAT) (Cysteine aminotransferase, cytoplasmic) (Cysteine transaminase, cytoplasmic) (cCAT) (Glutamate oxaloacetate transaminase 1) (Transaminase A). Biosynthesis of L-glutamate from L-aspartate or L-cysteine. (mybiosource.com)
  • Also known as Aspartate aminotransferase, cytoplasmic isozyme 1 (Transaminase A). Nitrogen metabolism. (mybiosource.com)
  • Also known as Aspartate aminotransferase, cytoplasmic isozyme 2 (Transaminase A). Encodes aspartate aminotransferase (Asp4). (mybiosource.com)
  • AST (aspartate aminotransferase or glutamate oxaloacetate transaminase) has been found in the cytoplasm and the mitochondria of cells that have been studied. (randox.com)
  • Aspartate transaminase is also known as L-aspartate-2-oxoglutarate aminotransferase, AST. (labpedia.net)
  • One of the aminotransferases is Aspartate aminotransferase (AST), or the old name was glutamic -oxaloacetic transaminase (GOT). (labpedia.net)
  • Alanine aminotransferase (ALT), formerly called serum glutamate pyruvate transaminase, or SGPT, is an enzyme necessary for energy production. (thefreedictionary.com)
  • Aspartate aminotransferase (AST), formerly called serum glutamic-oxaloacetic transaminase, or SGOT, is another enzyme necessary for energy production. (thefreedictionary.com)
  • An AST test measures the level of aspartate aminotransferase, also called AST or SGOT. (kidshealth.org)
  • Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit: High-Throughput Suitable Assay for Measuring ALT/SGPT Activity in Serum, Tissues, Cells etc. (fishersci.com)
  • The Aspartate Aminotransferase test (AST/SGOT test) has no fasting requirements. (requestatest.com)
  • If you need more than just Aspartate Aminotransferase (AST/SGOT) lab test - add more tests one by one. (findlabtest.com)
  • None Aspartate Aminotransferase (AST/SGOT) test cost minimal is in Health Testing Centers (Aspartate Aminotransferase (AST or SGOT)) with price $21.00. (findlabtest.com)
  • Aspartate Aminotransferase (AST/SGOT) test cost max is in LabReqs (Aspartate Aminotransferase (AST)) with price $39.00. (findlabtest.com)
  • 1. Cytoplasmic aspartate aminotransferase was isolated and purified from human heart with a final specific activity of 236 units/mg of protein. (clinsci.org)
  • Aspartate aminotransferase, cytoplasmic is an enzyme that in humans is encoded by the GOT1 gene. (wikipedia.org)
  • Aspartate aminotransferase may be measured as part of a liver function test. (nih.gov)
  • It's usually measured together with alanine aminotransferase (ALT) According to liver specialists, abnormal ALT results are more likely related to liver injury than abnormal AST results. (healthline.com)
  • The aspartate aminotransferase (AST) test is more effective than the alanine aminotransferase (ALT) test for detecting liver damage caused by alcohol abuse. (adventisthealthcare.com)
  • The Aspartate aminotransferase test measures levels of AST, an enzyme released into the blood when certain organs or tissues, particularly the liver and heart, are injured. (thefreedictionary.com)
  • This means that AST isn't as specific an indicator of liver damage as ALT (also known as alanine aminotransferase, another type of enzyme found almost entirely in the liver). (leebio.com)
  • The ratio of the serum aspartate to alanine amino-transferase levels (AST/ALT) is often used as a clue to the etiology of the underlying liver disease. (nih.gov)
  • Aspartate Aminotransferase (AST) is similar to alanine aminotransferase (ALT) in that it is another enzyme associated with liver parenchymal cells. (mediconsa.com)
  • Aspartate aminotransferase (AST) is an enzyme which is found in most of the body cells but mainly in the heart and liver. (1mg.com)
  • The levels of aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) in serum can help people diagnose body tissues especially the heart and the liver are injured or not. (elsevier.com)
  • The routine blood sample that examines liver function tests (LFTs), partly consist of alanine aminotransferase (ALT), aspartate aminotransferase (AST), the level of ALT/AST ratio (LSR), total bile acid (TBA), gamma glutamyltransferase (GGT), and lactate dehydrogenase (LDH). (biomedcentral.com)
  • Alanine aminotransferase measurements are used in the diagnosis and treatment of certain liver diseases (e.g., viral hepatitis and cirrhosis) and heart diseases. (cdc.gov)
  • Serum elevations of ALT activity are rarely observed except in parenchymal liver disease, since ALT is a more liver-specific enzyme than aspartate aminotransferase (AST). (cdc.gov)
  • Daily consumption of five to six cups of strong cafetiere coffee affects the integrity of liver cells as suggested by small increases in serum alanine aminotransferase concentration. (bmj.com)
  • Cafestol and kahweol seem to affect liver cells: short term intake of boiled coffee 5 or preparations rich in cafestol and kahweol 5 10 11 12 raise the serum concentration of alanine aminotransferase. (bmj.com)
  • For example, aminotransferases (which include ALT and AST) are notably elevated in liver damage caused by liver cell disease (hepatocellular disease). (thefreedictionary.com)
  • Additionally, in conjunction with the aspartate aminotransferase test (AST), it helps to distinguish between heart damage and liver tissue damage. (thefreedictionary.com)
  • The liver enzymes, alanine aminotransferase, aspartate aminotransferase and γ-glutamyl transpeptidase, were not significantly altered with exercise. (bmj.com)
  • Univariate analysis showed that advanced liver fibrosis was associated with lower platelets counts ( P =0.001) and higher aspartate aminotransferase (AST) ( P =0.001), alanine aminotransferase (ALT) ( P =0.022), APRI score ( P =0.001) and FIB-4 score ( P =0.001). (sciendo.com)
  • Acute viral hepatitis, drug or toxin induced liver disease and ischaemic liver injury are characterised by extremely elevated aminotransferase levels 3 . (randox.com)
  • Laboratory studies revealed an isolated increase in aspartate aminotransferase (AST)1 that prompted consultation with a hepatologist. (aacc.org)
  • Cardiovascular disease is characterized by the superior increase in aspartate aminotransferase (AST) to alanine aminotransferase (ALT). (hindawi.com)
  • At 25 degrees C, SsAspAT is less resistant to guanidinium chloride-induced denaturation than the cytosolic aspartate aminotransferase from pig heart (cpAspAT), which was chosen as a mesophilic counterpart in the thermodynamic analysis since it shares with SsAspAT the two-state unfolding mechanism. (nih.gov)
  • Porcine cytosolic aspartate aminotransferase is composed of two identical subunits of 412 residues. (nii.ac.jp)
  • Publications] Fukumoto,Y.: 'Structural and functional role of the amino-terminal region of porcine cytosolic aspartate aminotransferase.Catalytic and structural properties of enzyme derivatives truncated on the amino-terminal side. (nii.ac.jp)
  • Publications] Higaki,T.: 'Porcine cytosolic aspartate aminotransferase reconstituted with [4'-13C]pyridoxal phosphate.pH- and ligand-induced changes of the coenzyme observed by 13C NMR spectroscopy. (nii.ac.jp)
  • Major cytosolic isoenzyme controlling aspartate biosynthesis in the light. (mybiosource.com)
  • Recombinant full length protein (His-T7-tag) corresponding to Cow Aspartate Aminotransferase aa 2-413. (abcam.com)
  • Recombinant cow Aspartate Aminotransferase protein. (abcam.com)
  • Recombinant expression, purification, and characterization of three isoenzymes of aspartate aminotransferase from Arabidopsis thaliana. (semanticscholar.org)
  • We studied the diagnostic accuracy of two scoring systems, aspartate aminotransferase (AST) to platelet ratio index (APRI) and fibrosis 4 (Fib-4) score, which can be calculated from simple parameters obtained through blood tests in such situations. (sciendo.com)
  • abstract = "Binding isotope effects for l-aspartate reacting with the inactive K258A mutant of PLP-dependent aspartate aminotransferase to give a stable external aldimine intermediate are reported. (elsevier.com)
  • abstract = "Two refined crystal structures of aspartate aminotransferase from E.coli are reported. (elsevier.com)
  • The crystal structure of mitochondrial aspartate aminotransferase (mAAT) of chicken complexed with erythro-beta-hydroxyaspartate has been determined at 2.4 A resolution. (rcsb.org)
  • In enzymology, an aspartate-prephenate aminotransferase (EC 2.6.1.78) is an enzyme that catalyzes the chemical reaction L-arogenate + oxaloacetate ⇌ {\displaystyle \rightleftharpoons } prephenate + L-aspartate Thus, the two substrates of this enzyme are L-arogenate and oxaloacetate, whereas its two products are prephenate and L-aspartate. (wikipedia.org)
  • The systematic name of this enzyme class is L-arogenate:oxaloacetate aminotransferase. (wikipedia.org)
  • It catalyzes the reaction: Aspartate + ⍺-Ketoglutarate ⇌ Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L). In this Kit, an amino group is transferred of from aspartate to ⍺-ketoglutarate. (fishersci.com)
  • One unit will catalyze the transamination of one micromole of L-aspartate to alpha-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8. (leebio.com)
  • CATALYTIC ACTIVITY: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate. (mybiosource.com)
  • The amino group is enzymatically transferred by AST present in the specimen from aspartate to the carbon atom of 2-oxoglutarate yielding oxaloacetate and L-glutamate. (vitroscient.com)
  • Use Aspartate Aminotransferase for designing your calibrator/control reagent and for the synthesis of unnatural L-amino acids from α-keto acids. (roche.com)
  • Vitro AST reagent is intended for the in vitro quantitative determination of aspartate aminotransferase (EC 2.6.1.2) activity in serum on both automated and manual systems. (vitroscient.com)
  • Each Aspartate Aminotransferase Antibody Pair is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Aspartate Aminotransferase (AST) Antibody Pair for use in Sandwich ELISA assay development. (abbexa.com)
  • Aspartate aminotransferase from Sulfolobus solfataricus (SsAspAT) is an extremely thermophilic and thermostable dimeric enzyme which retains its structure and reaches maximal activity at 100 degrees C. The structural stability of this protein was investigated by coupling isothermally and thermally induced denaturation studies to molecular modeling. (nih.gov)
  • On starch-gel or polyacrylamide-gel electrophoresis of human serum, a supernumerary zone of aspartate aminotransferase activity may be demonstrated, migrating with the slow α 2 protein zone. (biochemj.org)
  • Structural basis for the catalytic activity of aspartate aminotransferase K258H lacking the pyridoxal 5'-phosphate-binding lysine residue. (uzh.ch)
  • In both strains of mice, plasma levels of alanine aminotransferase increased from week 1, when hepatic steatosis was also observed. (nih.gov)
  • Deciphering the role of aspartate and prephenate aminotransferase activities in plastid nitrogen metabolism. (semanticscholar.org)
  • Publications] Kuramitsu,S.: 'Pre-steady-state kinetics of Escherichia coli aspartate aminotransferase catalyzed reactions and thermodynamic aspects of its substrate specificity. (nii.ac.jp)
  • The P-5-P bound to the apoenzyme accepts the amino group from the first substrate, aspartate, to form enzyme-bound pyridoxine-5- phosphate, and the first reaction product is glutamate. (labpedia.net)
  • Arginine 292 forms a salt bridge with the beta-COOH of the substrate, aspartate. (scripps.edu)
  • As residue Arg292 is primarily responsible for the substrate charge specificity in the wild type enzyme, the mutant containing a charge reversal at this position might be expected to catalyze transamination of arginine as efficiently as the wild type enzyme effects transamination of aspartate [Cronin,C.N. and Kirsch,J.F. (1988) Biochemistry, 27, 4572-4579]. (elsevier.com)
  • This mutant does in fact prefer arginine over aspartate as a substrate, however, the rate of catalysis is much slower than that of the wild type enzyme with its physiological substrate, aspartate. (elsevier.com)
  • Biosynthesis of L-glutamate from L-aspartate or L-cysteine. (uniprot.org)
  • We recently reported that aspartate (Asp) biosynthesis in plant chloroplasts is catalyzed by two different Asp aminotransferases (AAT): a previously characterized eukaryote type and a prokaryote type (PT-AAT) similar to bacterial and archaebacterial enzymes. (plantphysiol.org)
  • Involved in aspartate biosynthesis and nitrogen metabolism. (mybiosource.com)
  • Plastidic aspartate aminotransferases and the biosynthesis of essential amino acids in plants. (semanticscholar.org)
  • MBS043425 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aspartate Aminotransferase (AST) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Aspartate aminotransferase (AST) is an enzyme that catalyzes the reversible transfer of an amino group between aspartate and α-ketoglutaric acid in the citric acid or Krebs cycle, a powerful and essential biochemical pathway for releasing stored energy. (unboundmedicine.com)
  • Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. (abcam.com)
  • The primary role of Tyr225 in the aspartate aminotransferase mechanism is to provide a hydrogen bond to stabilize the 3'O- functionality of bound pyridoxal phosphate. (scripps.edu)
  • To study the effects of prolonged intake of cafetiere coffee, which is rich in the diterpenes cafestol and kahweol, on serum aminotransferase and lipid concentrations. (bmj.com)
  • Mean changes in serum aminotransferase and lipid concentrations. (bmj.com)
  • Tyrosine aminotransferase, histidinol-phosphate aminotransferase, and aspartate aminotransferase are homologous proteins. (semanticscholar.org)
  • This enzyme catalyzes the reversible transfer of amino groups between an amino acid, and α-keto acids are called aminotransferase or transaminases. (labpedia.net)
  • The aspartate aminotransferase (AST) blood test measures the level of the enzyme AST in the blood. (medlineplus.gov)
  • The AST test may be done at the same time as a test for alanine aminotransferase, or ALT. (cigna.com)
  • Except for the medical supplies and life support products industries, most industries have been greatly impacted, and Aspartate Aminotransferase Test industries have also been greatly affected. (marketpublishers.com)
  • In the past few years, the Aspartate Aminotransferase Test market experienced a growth of xx, the global market size of Aspartate Aminotransferase Test reached xx million $ in 2020, of what is about xx million $ in 2015. (marketpublishers.com)
  • Therefore, we predict that the global epidemic will be basically controlled by the end of 2020 and the global Aspartate Aminotransferase Test market size will reach xx million $ in 2025, with a CAGR of xxx% between 2020-2025. (marketpublishers.com)
  • Turnaround time for the Aspartate Aminotransferase test is typically 1 business day. (requestatest.com)
  • Aspartate Aminotransferase (AST) test is used to evaluate a client with a suspected hepatocellular disease, injury, or inflammation (may also be used along with cardiac markers to evaluate coronary artery occlusive disease). (surenapps.com)
  • This test is done with alanine aminotransferase (ALT) test. (1mg.com)
  • Yüksek Kaliteli Biosystems test kitleri ve Cihazlarımızla kolaylıkla ve dakikalar içerisinde analiz sonuçlarına ulaşırsınız .Test sistemlerimiz, geniş uygulama alanları için en iyi şekilde belirlenmiş kitlerden ve Cihazlardan oluşmaktadır. (sesmedikal.com)
  • Publications] Yano,T.: 'The role of His143 in the catalytic mechanism of Escherichia coli aspartate aminotransferase. (nii.ac.jp)
  • Publications] Hayashi,H.: 'Effects of replacement of tryptophan-140 by phenylalanine or glycine on the function of Escherichia coli aspartate aminotransferase. (nii.ac.jp)
  • Chicken mitochondrial and Escherichia coli aspartate aminotransferases K258H, in which the active site lysine residue has been exchanged for a histidine residue, retain partial catalytic competence [Ziak et al. (uzh.ch)
  • Aminotransferases are a family of enzymes that catalyse the conversion of amino acids to 2-oxo-acids by the transfer of amino acids 1 . (randox.com)
  • To evaluate the degree of periodontal tissue destruction, aspartate aminotransferase (AST) levels in the gingival crevicular fluid (GCF) are utilized as a predictor of periodontal therapy. (springer.com)
  • We offer Aspartate Aminotransferase Antibody Pairs for use in common research applications: Immunoprecipitation, Sandwich ELISA, Western Blot. (novusbio.com)
  • Ratio of serum aspartate to alanine aminotransferase in chronic hepatitis. (nih.gov)
  • Characterization of five putative aspartate aminotransferase genes in the N2-fixing heterocystous cyanobacterium Anabaena sp. (semanticscholar.org)