Asialoglycoproteins: Endogenous glycoproteins from which SIALIC ACID has been removed by the action of sialidases. They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by adsorptive ENDOCYTOSIS they are delivered to LYSOSOMES for degradation. Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. They are elevated in serum of patients with HEPATIC CIRRHOSIS or HEPATITIS.Asialoglycoprotein Receptor: A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates the endocytosis of serum glycoproteins.OrosomucoidFetuins: A family of calcium-binding alpha-globulins that are synthesized in the LIVER and play an essential role in maintaining the solubility of CALCIUM in the BLOOD. In addition the fetuins contain aminoterminal cystatin domains and are classified as type 3 cystatins.Galactose Oxidase: An enzyme that oxidizes galactose in the presence of molecular oxygen to D-galacto-hexodialdose. It is a copper protein. EC 1.1.3.9.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Endocytosis: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.Receptors, Cell Surface: Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.Receptors, Immunologic: Cell surface molecules on cells of the immune system that specifically bind surface molecules or messenger molecules and trigger changes in the behavior of cells. Although these receptors were first identified in the immune system, many have important functions elsewhere.Lysosomes: A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Kinetics: The rate dynamics in chemical or physical systems.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.Second Messenger Systems: Systems in which an intracellular signal is generated in response to an intercellular primary messenger such as a hormone or neurotransmitter. They are intermediate signals in cellular processes such as metabolism, secretion, contraction, phototransduction, and cell growth. Examples of second messenger systems are the adenyl cyclase-cyclic AMP system, the phosphatidylinositol diphosphate-inositol triphosphate system, and the cyclic GMP system.Spermatids: Male germ cells derived from the haploid secondary SPERMATOCYTES. Without further division, spermatids undergo structural changes and give rise to SPERMATOZOA.Testis: The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.Spermatogenesis: The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Inventions: A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.Mannose-Binding Lectin: A specific mannose-binding member of the collectin family of lectins. It binds to carbohydrate groups on invading pathogens and plays a key role in the MANNOSE-BINDING LECTIN COMPLEMENT PATHWAY.Mannose-Binding Lectins: A subclass of lectins that are specific for CARBOHYDRATES that contain MANNOSE.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Phorbol Esters: Tumor-promoting compounds obtained from CROTON OIL (Croton tiglium). Some of these are used in cell biological experiments as activators of protein kinase C.Superior Sagittal Sinus: The long large endothelium-lined venous channel on the top outer surface of the brain. It receives blood from a vein in the nasal cavity, runs backwards, and gradually increases in size as blood drains from veins of the brain and the DURA MATER. Near the lower back of the CRANIUM, the superior sagittal sinus deviates to one side (usually the right) and continues on as one of the TRANSVERSE SINUSES.Cathepsin L: A ubiquitously-expressed cysteine protease that plays an enzymatic role in POST-TRANSLATIONAL PROTEIN PROCESSING of proteins within SECRETORY GRANULES.Glioblastoma: A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.Area Under Curve: A statistical means of summarizing information from a series of measurements on one individual. It is frequently used in clinical pharmacology where the AUC from serum levels can be interpreted as the total uptake of whatever has been administered. As a plot of the concentration of a drug against time, after a single dose of medicine, producing a standard shape curve, it is a means of comparing the bioavailability of the same drug made by different companies. (From Winslade, Dictionary of Clinical Research, 1992)Half-Life: The time it takes for a substance (drug, radioactive nuclide, or other) to lose half of its pharmacologic, physiologic, or radiologic activity.Myelodysplastic Syndromes: Clonal hematopoietic stem cell disorders characterized by dysplasia in one or more hematopoietic cell lineages. They predominantly affect patients over 60, are considered preleukemic conditions, and have high probability of transformation into ACUTE MYELOID LEUKEMIA.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.Size Perception: The sensory interpretation of the dimensions of objects.Molecular Weight: The sum of the weight of all the atoms in a molecule.Dose-Response Relationship, Radiation: The relationship between the dose of administered radiation and the response of the organism or tissue to the radiation.Nitrate Reductase (NADH): An NAD-dependent enzyme that catalyzes the oxidation of nitrite to nitrate. It is a FLAVOPROTEIN that contains IRON and MOLYBDENUM and is involved in the first step of nitrate assimilation in PLANTS; FUNGI; and BACTERIA. It was formerly classified as EC 1.6.6.1.Ultracentrifugation: Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Technetium Tc 99m Diethyl-iminodiacetic Acid: A nontoxic radiopharmaceutical that is used in the clinical evaluation of hepatobiliary disorders in humans.Radiopharmaceuticals: Compounds that are used in medicine as sources of radiation for radiotherapy and for diagnostic purposes. They have numerous uses in research and industry. (Martindale, The Extra Pharmacopoeia, 30th ed, p1161)Liver Function Tests: Blood tests that are used to evaluate how well a patient's liver is working and also to help diagnose liver conditions.Oxamniquine: An anthelmintic with schistosomicidal activity against Schistosoma mansoni, but not against other Schistosoma spp. Oxamniquine causes worms to shift from the mesenteric veins to the liver where the male worms are retained; the female worms return to the mesentery, but can no longer release eggs. (From Martindale, The Extra Pharmacopoeia, 31st ed, p121)Technetium Tc 99m Pentetate: A technetium imaging agent used in renal scintigraphy, computed tomography, lung ventilation imaging, gastrointestinal scintigraphy, and many other procedures which employ radionuclide imaging agents.Pennisetum: A plant genus of the family POACEAE. The seed is one of the millets used in EDIBLE GRAIN. It contains vitexin. The common name of buffelgrass is also used for CENCHRUS.Cenchrus: A plant genus of the family POACEAE. The common name of buffelgrass is also used for PENNISETUM.Coronary Artery Disease: Pathological processes of CORONARY ARTERIES that may derive from a congenital abnormality, atherosclerotic, or non-atherosclerotic cause.Myocardial Infarction: NECROSIS of the MYOCARDIUM caused by an obstruction of the blood supply to the heart (CORONARY CIRCULATION).Coronary Angiography: Radiography of the vascular system of the heart muscle after injection of a contrast medium.Coronary Vessels: The veins and arteries of the HEART.Immunotherapy: Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Neuroblastoma: A common neoplasm of early childhood arising from neural crest cells in the sympathetic nervous system, and characterized by diverse clinical behavior, ranging from spontaneous remission to rapid metastatic progression and death. This tumor is the most common intraabdominal malignancy of childhood, but it may also arise from thorax, neck, or rarely occur in the central nervous system. Histologic features include uniform round cells with hyperchromatic nuclei arranged in nests and separated by fibrovascular septa. Neuroblastomas may be associated with the opsoclonus-myoclonus syndrome. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2099-2101; Curr Opin Oncol 1998 Jan;10(1):43-51)Medical Oncology: A subspecialty of internal medicine concerned with the study of neoplasms.Software: Sequential operating programs and data which instruct the functioning of a digital computer.

Stabilization of poly-L-lysine/DNA polyplexes for in vivo gene delivery to the liver. (1/431)

We are developing a self-assembling non-viral in vivo gene delivery vehicle based on poly-l-lysine and plasmid DNA. We have characterized poly-l-lysines of different chain lengths for DNA condensation and strength of DNA binding. Poly-l-lysine chains >20 residues bound DNA efficiently in physiological saline, while shorter chains did not. Attachment of asialoorosomucoid to PLL increased the PLL chain length required for efficient DNA binding in saline and for efficient DNA condensation. By electron microscopy, poly-l-lysine/DNA polyplexes appeared as toroids 25-50 nm in diameter or rods 40-80 nm long; conjugation of asialoorosomucoid to the polylysine component increased the size of resulting polyplexes to 50-90 nm. In water, poly-l-lysine and asialoorosomucoid-PLL polyplexes have effective diameters of 46 and 87.6 nm, respectively. Polyplexes containing only poly-l-lysine and DNA aggregated in physiological saline at all charge ratios and aggregated at neutral charge ratios in water. Attachment of asialoorosomucoid lessened, but did not eliminate, the aggregation of PLL polyplexes, and did not result in efficient delivery of polyplexes to hepatocytes. Conjugation of polyethylene glycol to poly-l-lysine sterically stabilized resulting polyplexes at neutral charge ratios by shielding the surfaces. For efficient in vivo gene delivery, polyplexes will need to be sterically stabilized to prevent aggregation and interaction with serum components.  (+info)

Nucleotide exchange in genomic DNA of rat hepatocytes using RNA/DNA oligonucleotides. Targeted delivery of liposomes and polyethyleneimine to the asialoglycoprotein receptor. (2/431)

Chimeric RNA/DNA oligonucleotides have been shown to promote single nucleotide exchange in genomic DNA. A chimeric molecule was designed to introduce an A to C nucleotide conversion at the Ser365 position of the rat factor IX gene. The oligonucleotides were encapsulated in positive, neutral, and negatively charged liposomes containing galactocerebroside or complexed with lactosylated polyethyleneimine. The formulations were evaluated for stability and efficiency in targeting hepatocytes via the asialoglycoprotein receptor. Physical characterization and electron microscopy revealed that the oligonucleotides were efficiently encapsulated within the liposomes, with the positive and negative formulations remaining stable for at least 1 month. Transfection efficiencies in isolated rat hepatocytes approached 100% with each of the formulations. However, the negative liposomes and 25-kDa lactosylated polyethyleneimine provided the most intense nuclear fluorescence with the fluorescein-labeled oligonucleotides. The lactosylated polyethyleneimine and the three different liposomal formulations resulted in A to C conversion efficiencies of 19-24%. In addition, lactosylated polyethyleneimine was also highly effective in transfecting plasmid DNA into isolated hepatocytes. The results suggest that both the liposomal and polyethyleneimine formulations are simple to prepare and stable and give reliable, reproducible results. They provide efficient delivery systems to hepatocytes for the introduction or repair of genetic mutations by the chimeric RNA/DNA oligonucleotides.  (+info)

Copper and zinc ions differentially block asialoglycoprotein receptor-mediated endocytosis in isolated rat hepatocytes. (3/431)

Asialoglycoprotein receptors on hepatocytes lose endocytic and ligand binding activity when hepatocytes are exposed to iron ions. Here, we report the effects of zinc and copper ions on the endocytic and ligand binding activity of asialoglycoprotein receptors on isolated rat hepatocytes. Treatment of cells at 37 degrees C for 2 h with ZnCl2 (0-220 microM) or CuCl2 (0-225 microM) reversibly blocked sustained endocytosis of 125I-asialoorosomucoid by up to 93% (t1/2 = 62 min) and 99% (t1/2 = 54 min), respectively. Cells remained viable during such treatments. Zinc- and copper-treated cells lost approximately 50% of their surface asialoglycoprotein receptor ligand binding activity; zinc-treated cells accumulated inactive asialoglycoprotein receptors intracellularly, whereas copper-treated cells accumulated inactive receptors on their surfaces. Cells treated at 4 degrees C with metal did not lose surface asialoglycoprotein receptor activity. Exposure of cells to copper ions, but not to zinc ions, blocked internalization of prebound 125I-asialoorosomucoid, but degradation of internalized ligand and pinocytosis of the fluid-phase marker Lucifer Yellow were not blocked by metal treatment. Zinc ions reduced diferric transferrin binding and endocytosis on hepatocytes by approximately 33%; copper ions had no inhibitory effects. These findings are the first demonstration of a specific inhibition of receptor-mediated endocytosis by non-iron transition metals.  (+info)

Asialoglycoprotein receptor scintigraphy in evaluation of auxiliary partial orthotopic liver transplantation. (4/431)

The purpose of this study was to evaluate asialoglycoprotein receptor scintigraphy in the post-transplant monitoring of liver graft and native liver functions in recipients of auxiliary partial orthotopic liver transplantation (APOLT) from living donors. METHODS: We performed 36 asialoglycoprotein receptor scintigraphies on 13 patients who had undergone APOLT for noncirrhotic metabolic liver diseases or for small-for-size grafts. The portal vein of the native liver was separated in 12 patients. Anterior dynamic images including the heart and both livers were obtained for 16 min after intravenous injection of 99mTc-diethylenetriamine pentaacetic acid-galactosyl human serum albumin (GSA), and thereafter static SPECT images of both livers were obtained. Uptake rates from the blood to the graft and to the native liver were determined separately by Patlak plot graphical analysis. Relative uptake of GSA by the graft was calculated from transverse SPECT images. The relative volume of the graft liver was determined by CT. RESULTS: The relative uptake of GSA by the graft was higher or increased more rapidly than the relative volume of the graft in 8 of 11 patients with no severe complications concerning the graft. The relative uptake by severely damaged graft liver in 2 patients was much lower than the relative volume. The uptake rate of GSA by the graft was low in these 2 patients. The uptake rate by the native liver decreased when the portal vein was separated. CONCLUSION: The relative uptake of GSA was a better indicator of graft liver function than was anatomic volume. The uptake rate provided additional independent information of each liver. Asialoglycoprotein receptor scintigraphy is useful for distinguishing and monitoring the graft and native liver functions in patients who had undergone APOLT.  (+info)

Molecular cloning and expression of Galbeta1,3GalNAc alpha2, 3-sialyltransferase from human fetal liver. (5/431)

Based on the sequences of the highly conserved segments in the previously cloned sialyltransferases, a cDNA encoding Galbeta1, 3GalNAc alpha2,3-sialyltransferase (SIATFL) has been isolated from human fetal liver. Expression analysis of the gene has been performed with various carcinoma cell lines, fetal tissues, fetal and adult liver and both hepatoma and the surrounding tissue from the same liver. The SIATFL gene was expressed poorly in fetal liver and in adult liver, slightly in hepatoma and highly in the surrounding tissue of hepatoma. The cDNA encoding the putative active domain was expressed in COS-1, Escherichia coli, and Pichia pastoris. The recombinant protein expressed in COS-1 could catalyse the transfer of NeuAc from CMP-NeuAc to asialo-fetuin. No enzyme activity was detected with a 32-kDa protein in E. coli and both 32-kDa and 41-kDa proteins in P. pastoris. These results suggested that correct glycosylation of the enzyme might play a key role in its folding that may be directly related to the enzymatic activity.  (+info)

Characterization of recombinant and plant-derived mistletoe lectin and their B-chains. (6/431)

Mistletoe lectin I (pML) and its isoforms ML II and III constitute the active principle in extract preparations from mistletoe, commonly used as immunomodulator in adjuvant tumour therapy. The heterodimeric disulfide-linked cytotoxic protein is classified as type II ribosome inactivating protein (RIP). Recently, the sequence coding for the mistletoe lectin prepro-protein was identified and the existence of a single intron-free gene was shown [Eck, J., Langer, M., Mockel, B., Baur, A., Rothe, M., Zinke, H. & Lentzen, H. (1999) Eur. J. Biochem. 264, 775-784]. The aim of this study was to prepare pure and homogeneous rMLB-chain as well as rML heterodimer for studying the carbohydrate binding specificity of recombinant versus natural protein and its contribution to the observed cytotoxic effect. Expression in E. coli resulted in the production of insoluble protein (inclusion bodies). A procedure for generating correctly folded, biochemically and biologically active rMLB was established starting from the insoluble single chain. Carbohydrate binding and specificity of pMLB and rMLB were analysed by a competitive enzyme linked lectin assay (ELLA). Asialofetuin was able to compete with binding of both chains (50% at 0.8 microM). The specificity of the B-chains to lactose was more distinct with halfmaximal competition at 4.9 mM (pMLB) and > 90 mM (rMLB), respectively. Furthermore, in a coassociation process rMLA- and rMLB inclusion bodies were associated in one step by defined dilution yielding active rML-heterodimer. The activities of recombinant (rML) and plant derived mistletoe lectin (pML) were compared. Cytotoxicity was determined using MOLT-4 cells and enzymatic rRNA N-glycosidase activity was measured in a coupled transcription/translation assay. The IC50 values of the two heterodimers were similar in both assays; rMLB-chain did not show any cytotoxic effect. In the ELLA with lactose as a competitor 50% competition of binding to asialofetuin was achieved at 1.6 mM (rML) and 1.8 mM (pML). Hence, using three different assays we found no significant differences between the recombinant protein and the glycosylated form of ML. Comparing the biological activities of the single chains with those of the heterodimer we conclude, that both, lectin activity and the rRNA N-glycosidase activity, are prerequisites for the cytotoxic effects on target cells.  (+info)

Evaluation of the components of the chylomicron remnant removal mechanism by use of the isolated perfused mouse liver. (7/431)

The isolated perfused mouse liver was utilized to evaluate the relative contribution of various molecules believed to participate in the removal of chylomicron remnants by the liver. Sixty percent of asialofetuin was removed from the perfusate per pass; bovine serum albumin was not removed. Normal mouse livers removed chylomicron remnants more efficiently (40-50%/pass) than nascent chylomicrons (10-20%/pass). The fractional removal rate of remnants decreased as their concentration in the perfusate increased demonstrating saturability. Remnant removal by livers of low density lipoprotein receptor-deficient (LDLRD) mice paralleled that of normal mice at low remnant concentrations (0.05, 0.2 microg protein/ml); as concentration increased (4-16 microg protein/ml), removal by LDLRD livers was reduced. About 50% of the capacity to remove remnants was due to the LDL receptor. The role of the LDLR-related protein (LRP) was estimated using the receptor-associated protein (RAP). Four microg/ml of RAP inhibited only LRP; it reduced the removal of remnants by 30-40% in normal livers. When RAP was included in the perfusate of LDLRD livers, remnant removal persisted but was diminished, particularly late in the perfusion; the capacity was approximately 30% of controls. The present study has established that there is more than one mechanism operating for the removal of chylomicron remnants by the liver, provides estimates of the concentration of each to the removal of remnants, and indicates a method for further studies. It is concluded that in normal livers, the LDL receptor has the greatest capacity for removing chylomicron remnants. The LRP contributes to the process as well and a third component, perhaps "sequestration," accounts for up to 30% of the capacity for the initial removal of chylomicron remnants.  (+info)

Fluid phase endocytosis and galactosyl receptor-mediated endocytosis employ different early endosomes. (8/431)

Endocytosis may originate both in coated pits and in uncoated regions of the plasma membrane. In hepatocytes it has been shown that fluid phase endocytosis (here defined as 'pinocytosis') is unaffected by treatments that arrest coated pit-mediated endocytosis, indicating that pinocytosis is primarily a clathrin-independent process. In this study we have tried to determine possible connections between pinocytosis and clathrin-dependent endocytosis in rat hepatocytes by means of subcellular fractionation, electron microscopy, and by assessing the influence of inhibitors of clathrin-dependent endocytosis on pinocytosis. As marker for clathrin-dependent endocytosis was used asialoorosomucoid (AOM) labelled with [(125)I]tyramine cellobiose ([(125)I]TC). [(125)I]TC-labelled bovine serum albumin ([(125)I]TC-BSA) was found to be a useful marker for pinocytosis. Its uptake in the cells is not saturable, and any remnants of [(125)I]TC-BSA associated with the cell surface could be removed by incubating the cells with 0.3% pronase at 0 degrees C for 60 min. The data obtained by electron microscopy and by subcellular fractionation suggested that early after initiation of uptake (<15 min) [(125)I]TC-BSA and [(125)I]TC-AOM were present in different endocytic vesicles. The two probes probably join prior to their entrance in the lysosomal compartment. The relation between endocytosis via coated pits and pinocytosis was also studied with techniques that induced a selective density shift either in the clathrin-dependent pathway (by AOM-HRP) or in the pinocytic pathway (by allowing uptake of AuBSA). Both treatments indicated that the two probes ([(125)I]TC-AOM and [(125)I]TC-BSA) were early after uptake, at least partly, in separate endocytic compartments. The different distribution of the fluid phase marker and the ligand (internalised via coated pits) was not due to a difference in the rate at which they enter a later compartment, since a lowering of the incubation temperature to 18 degrees C, which should keep the probes in the early endosomes, did not affect their early density distribution. Incubation of cells in a hypertonic medium reduced uptake both of [(125)I]TC-AOM and [(125)I]TC-BSA; the uptake of [(125)I]TC-AOM was, however, reduced much more than that of the fluid phase marker. This finding supports the notion that the two probes enter the cells via different routes.  (+info)

The ability of a peptide vaccine to stimulate T cells in vivo might be improved by specifically targeting the peptide to dendritic cells (DC). The C-type lectin Macrophage Galactose-type lectin, MGL (CD301), has been shown to bind to N-acetyl-galactosamine (GalNAc) and small peptides bearing O-linked GalNAc. Synthetic GalNAc can be produced using relatively simple organic chemistry when compared with the complicated branched sugars that are recognised by other C-type lectins. MGL therefore represents a promising target for the design of synthetic peptide vaccines. We have identified that antigen-presenting cells in human skin express MGL and have confirmed that CD14+ dermal DCs might be targeted via MGL. The intracellular fate of MGL following internalisation was tracked by confocal microscopy. MGL traffics through early endosomes to late endosomes/lysosomes, and colocalises with MHC class I and class II. Synthetic glycopeptides were produced incorporating either native O-linked GalNAc or GalNAc ...
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IFDB is a game catalog and recommendation engine for Interactive Fiction, also known as Text Adventures. IFDB is a collaborative, wiki-style community project. Members can contribute game listings, reviews, recommendations, and more.
Xiu-Sheng Miao, Pengfei Song, Ronald E. Savage, Caiyun Zhong, Rui-Yang Yang, Darin Kizer, Hui Wu, Erika Volckova, Mark A. Ashwell, Jeffrey G. Supko, Xiaoying He and Thomas C. K. Chan ...
Dave Weigel writes the smartest thing Ive read about the Sarah Palin phenomenon in some time. The problem is that Palin has put the political press in a submissive position, one in which the only information it prints about her...
TY - JOUR. T1 - Asialoglycoprotein receptor phosphorylation and receptor-mediated endocytosis in hepatoma cells. Effect of phorbol esters. AU - Fallon, R. J.. AU - Schwartz, A. L.. PY - 1988/1/1. Y1 - 1988/1/1. N2 - The asialoglycoprotein (ASGP) receptor on Hep G2 cells undergoes constitutive recycling and ligand endocytosis in the presence of phorbol dibutyrate, at a 50% reduced rate relative to control cells (Fallon, R. J., and Schwartz, A. L. (1986) J. Biol. Chem. 261, 15081-15089). The relevance of receptor phosphorylation to these events was investigated by selective immunoprecipitation of surface receptors with polyclonal anti-human ASGP antiserum and pulse-chase labeling with [32P]orthophosphate to identify subcellular locations of initial receptor phosphorylation events as well as the eventual fate of phosphorylated receptor during recycling. The surface immunoprecipitation method recovers greater than 95% of surface ASGP receptors and only 5% or less of intracellular ...
0041]It is presently preferred to isolate E1 and/or E2 asialoglycoproteins from crude cell lysates by incubation with an immobilized mannose-binding protein, particularly a lectin such as ConA or GNA. Cells are lysed, e.g., by mechanical disruption in a hypotonic buffer followed by centrifugation to prepare a post-nuclear lysate, and further centrifuged to obtain a crude microsomal membrane fraction. The crude membrane fraction is subsequently solubilized in a buffer containing a detergent, such as Triton X-100, NP40, or the like. This detergent extract is further clarified of insoluble particulates by centrifugation, and the resulting clarified lysate incubated in a chromatography column comprising an immobilized mannose-binding protein, preferably GNA bound to a solid support such as agarose or Sepharose® for a period of time sufficient for binding, typically 16 to 20 hours. The suspension is then applied to the column until E1/E2 begins to appear in the eluent, then incubated in the column ...
Alterations in protein synthesis in primary cultured rat liver parenchymal cells were examined after their exposure to the potent carcinogens, polychlorinated biphenyl (PCB) congeners. Co-planar PCB congeners (3,4,5,3′,4′-PCB and 3,4,5,3′,4′,5′-PCB) (10 nM) induced a protein, the Mr of which was 25,000 (25 k protein) under denaturing conditions. However, non-co-planar PCB congeners and several xenobiotics, which induce microsomal proteins, did not induce the 25 k protein. By using immunoblotting, the 25 k protein was identified as glutathione S-transferase P-form (GST-P, 7-7, EC 2.5.1.18).. ...
Asialoglycoprotein Receptor 1兔多克隆抗体(ab42488)可与大鼠, 人样本反应并经WB, ICC/IF实验严格验证,被2篇文献引用并得到2个独立的用户反馈。
Residualizing labels are tracers which remain in lysosomes after uptake and catabolism of the carrier protein and have been especially useful for studies on the sites of plasma protein degradation. Thus far these labels have contained radioactive reporters such as 3H or 125I. In the present paper we describe a fluorescent residualizing label, NN-dilactitol-N′-fluoresceinylethylenediamine (DLF). Modification of asialofetuin (ASF) or rat serum albumin (RSA) with DLF affected neither their normal kinetics of clearance from the rat circulation nor their normal tissue sites of uptake and degradation. After injection of DLF-ASF, fluorescent degradation products were recovered nearly quantitatively in liver and retained with a half-life of about 2 days. Fluorescent degradation products from DLF-RSA were recovered in skin and muscle, and were localized in fibroblasts by fluorescence microscopy. These results confirm previous studies with radioactive residualizing labels in which fibroblasts in ...
Rabbit Polyclonal Anti-Asialoglycoprotein Receptor 2 Antibody. Validated: WB, IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
DNA molecules complexed with an asialoglycoprotein-polycation conjugate, consisting of asialoorosomucoid (ASOR) coupled to poly-l-lysine, can enter hepatocytes which bear receptors for ASOR. We used this receptor-mediated DNA delivery system to deliver plasmid DNA encoding glycoprotein D (gD) of herpes simplex virus type 1 to ASOR-positive cells. Maximum expression of gD protein was seen at 3 days after injection of this preparation in approximately 13% of cells from BALB/c mice [hepatocytes from mice injected intravenously (i.v.) or peritoneal exudate cells from mice injected intraperitoneally (i.p.)]. In comparison with mice injected with either the plasmid vector alone or the gD-containing plasmid uncomplexed to ASOR, mice immunized with gD-containing plasmid complexed with ASOR-poly-l-lysine induced marked antigen-specific CTL responses. BALB/c mice immunized with gD-DNA developed a T-cell-mediated CTL response against target cells expressing gD and MHC class II glycoproteins, but not against cells
Carbohydrate-oligonucleotide conjugates have become increasingly important in recent years, particularly with regard to an increased uptake and cell availability of siRNA and antisense oligonucleotides. Of particular interest here is N-acetylgalactosamine (GalNAc), which can bind to the asialoglycoprotein receptor (ASGPR) in hepatocytes.. The following picture shows some examples of the attachment of carbohydrates (sugars) to oligonucleotides via different linker structures:. ...
Carbohydrate-oligonucleotide conjugates have become increasingly important in recent years, particularly with regard to an increased uptake and cell availability of siRNA and antisense oligonucleotides. Of particular interest here is N-acetylgalactosamine (GalNAc), which can bind to the asialoglycoprotein receptor (ASGPR) in hepatocytes.. The following picture shows some examples of the attachment of carbohydrates (sugars) to oligonucleotides via different linker structures:. ...
The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid…
1FIH: Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor.
1FIF: Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor.
Acetyl-LDL (Ac-LDL) bound to transformed mouse macrophage J774 cells in a high affinity, saturable and specific manner. When cells were cultured for 24h in the presence of tunicamycin such that incorporation of N-linked sugars into protein but not protein synthesis itself was inhibited significantly, the binding characteristics of Ac-LDL to the cells were unaltered. In this respect the Ac-LDL receptor of J774 cells is similar to the asialoglycoprotein receptor of HepG2 cells. ...
French Catholic philosopher Jacques Maritain once wrote that Americans "are the least materialist among the modern peoples which have attained the industrial stage.". Drawing on this sentiment, George Weigel argues that although materialism may reign in America more than it once did, "there remains a link between money-making and idealism in these United States that is distinctive, and perhaps even unique.". Pointing to President Calvin Coolidge (no fan of materialism), Weigel emphasizes that Coolidges famous line-"the chief business of the American people is business"-shouldnt be taken by itself. For Coolidge, and for most Americans (even today), promoting the dignity-conferring effects of business is part of a larger, deeper idealism.. As Weigel explains:. As for wealth, consider Silent Cals remarks at the end of the same speech: "We make no concealment of the fact that we want wealth, but there are many other things that we want very much more. We want peace and honor, and that charity ...
Welcome to Biology 1510 for Fall 2017! Select course readings from the menus above by Module: Evolution Ecology Molecules and Metabolism Genetics Integrative Human Health Lecture materials can be downloaded from t-square Instructions for the group video project will be … Continue reading →. ...
K. Schneeberger, Hagmann, J., Ossowski, S., Warthmann, N., Gesing, S., Kohlbacher, O., and Weigel, D., "Simultaneous alignment of short reads against multiple genomes", Genome Biol, vol. 10, p. R98, 2009. ...
Fenner L.; Brinkhof M.W.G.; Keiser O.; Weigel R.; Cornell M.; Moultrie H.; Prozesky H.; Technau K.; Eley B.; Vaz P.; Pascoe M.; Giddy J.; Van Cutsem G.; Wood R.; Egger M.; Davies M.-A. (2010) ...
A number of genes encoding C-type lectin molecules have been mapped to the natural killer gene complex (NKC) at the distal region of mouse chromosome 6 and to a syntenic region on human chromosome 12p12-p13. In addition to those receptors which regulate NK cell function, related structures expressed on other cells types have also been localized to this chromosomal region. Among these are a number of recently characterized genes, including macrophage C-type lectin (MCL), macrophage-inducible C-type lectin (Mincle), dendritic cell immunoreceptor (DCIR) and dendritic cell-associated lectin-2 (Dectin-2). The amino acid sequences comprising the single C-type lectin domains of MCL, Mincle, DCIR and Dectin-2 are shown here to be closely related to each other. These molecules show overall similarity to two groups of animal C-type lectins, groups II and V, which demonstrate type II transmembrane topology. In this study, sequence analysis suggests that MCL, Mincle, DCIR and Dectin-2 represent a subset of group II
Neurobiology of Australian Marsupials von Ashwell Ashwell und Buchbewertungen gibt es auf ReadRate.com. Bücher können hier direkt online erworben werden.
The major and minor forms of the asialoglycoprotein receptor (ASGP-R), designated in the rat as RHL-1 and RHL-2/3, respectively, have traditionally been considered to be expressed exclusively in hepatic parenchymal cells. Northern blot analysis now demonstrates that rat and mouse testis express a receptor similar to the RHL-1 ASGP-R but not the RHL-2/3 receptor. In situ hybridization studies demonstrate that late-stage mouse and rat spermatids are the testicular cells that express the RHL-1 ASGP-R. The rat spermatid RHL-1 receptor has functional binding capability, with a KD of approximately 1.4 x 10(-8) M, and similar characteristics, compared with the hepatic RHL-1 receptor, regarding ligand binding specificity and ion dependence. These findings have major implications for therapeutic procedures attempting to target cytotoxic agents or DNA to hepatocytes using the ASGP-R. In addition, these findings demonstrate that late-stage spermatids are transcriptionally active and suggest that the RHL-1 ...
The number of Asialoglycoprotein (ASGP) receptors on the hepatocytes of patients with liver disease is reduced and is thus considered a good indicator for the evaluation of liver function. ASGP receptor-targeted radiopharmaceuticals permit a non-invasive way to evaluate total and regional hepatic function and hepatic functional reserve visually and quantitatively. Over the past three decades, a variety of ASGP receptor-targeted probes have been developed with different molecular backbones (albumin, polymer, small-molecular-weight ligand), different glycol-residues (galactose, lactose, N-acetyl-galactosamine) and different chelating systems suitable for radiolabeling with SPECT isotopes (Tc-99m, In-111, Ga-67, (131)/I-125, Sm-153) and PET isotopes (Ga-68, F-18). In this review, we present an overview of ASGP receptor-targeted radiopharmaceuticals, discuss their chemistry, biodistribution, catabolism and challenge as well as application for measurement of liver function ...
William Ashwell Shenstone FIC FRS (1 December 1850 in Wells-next-the-Sea, Norfolk, England - 3 February 1908 in Mullion, Cornwall) was a chemist, schoolmaster and published author. Shenstone was born on 1 December 1850 to James Burt Byron Shenstone, a pharmaceutical chemist in Colchester, and his wife Jemima; he was named after the poet William Shenstone, a distant relative. He was educated at Colchester Royal Grammar School, and later as an apprentice to his father, though, during the latter, he was caught in a fire and suffered from pains throughout his life. He married Jane Mildred, the daughter of the rector of Wootton, on 27 December 1883, with whom he had two children, a son and a daughter. Shenstone died on 3 February 1908 at the Polurrian Hotel, Mullion, Cornwall, at the age of fifty-eight. By trade a schoolmaster, Shenstone joined Clifton College, Bristol, as assistant to William Tilden in 1874, before being appointed science master at Taunton School the next year, and in 1877 at Exeter ...
Exactly. News organizations dont hire conservatives. Therefore, conservatives dont seek careers in journalism and instead become lawyers or accountants or stock brokers or whatever.. Ergo, none of the people making decisions in MSM newsrooms are conservatives. Where set A = "employees of news organizations" and set B = "conservatives," we see that as the closer set AB approaches to zero, the more likely journalists are to view conservatives as The Other, and vice-versa.. If you think such a situation is a good thing, then youre obviously congratulating yourself about Dave Weigels downfall, and you dont want to hear the explanation of my disagreement.. UPDATE VI: Not to say there isnt an upside: Ezra Klein has deep-sixed Journolist as a result of the Weigel affair. Little Miss Attila says the list had become "blackmail fodder.". I spoke to Weigel by phone this afternoon. Hes fine, and is currently deciding which of three or four job offers to accept. He admits that he succumbed to hubris, ...
RESULTS: We found a rare noncoding 12-base-pair (bp) deletion (del12) in intron 4 of ASGR1, which encodes a subunit of the asialoglycoprotein receptor, a lectin that plays a role in the homeostasis of circulating glycoproteins. The del12 mutation activates a cryptic splice site, leading to a frameshift mutation and a premature stop codon that renders a truncated protein prone to degradation. Heterozygous carriers of the mutation (1 in 120 persons in our study population) had a lower level of non-HDL cholesterol than noncarriers, a difference of 15.3 mg per deciliter (0.40 mmol per liter) (P=1.0×10(-16)), and a lower risk of coronary artery disease (by 34%; 95% confidence interval, 21 to 45; P=4.0×10(-6)). In a larger set of sequenced samples from Icelanders, we found another loss-of-function ASGR1 variant (p.W158X, carried by 1 in 1850 persons) that was also associated with lower levels of non-HDL cholesterol (P=1.8×10(-3 ...
Presentation of sialic acid (Sia) varies among different tissues and organs within each species, and between species. This diversity has biologically important consequences regarding the recognition of cells by "xeno" antibodies (Neu5Gc vs Neu5Ac). Sia also plays a central role in inflammation by influencing binding of the asialoglycoprotein receptor 1 (ASGR-1), Siglec-1 (Sialoadhesin), and cellular interactions mediated by the selectin, integrin, and galectin receptor families. This review will focus on what is known about basic Sia structure and function in association with xenotransplantation, how changes in sialylation may occur in this context (through desialylation or changes in sialyltransferases), and how this fundamental pathway modulates adhesive and cell activation pathways that appear to be particularly crucial to homeostasis and inflammation for xenografts ...
Chao, D. (Creator), Borevitz, J. O. (Creator), Atwell, S. (Creator), Huang, Y. S. (Creator), Vilhjalmsson, B. J. (Creator), Willems, G. (Creator), Horton, M. (Creator), Li, Y. (Creator), Meng, D. (Creator), Platt, A. (Creator), Tarone, A. M. (Creator), Hu, T. T. (Creator), Jiang, R. (Creator), Muliyati, N. W. (Creator), Zhang, X. (Creator), Amer, M. A. (Creator), Baxter, I. (Creator), Brachi, B. (Creator), Chory, J. (Creator), Dean, C. (Creator), Debieu, M. (Creator), de Meaux, J. (Creator), Ecker, J. R. (Creator), Faure, N. (Creator), Kniskern, J. M. (Creator), Jones, J. D. G. (Creator), Michael, T. (Creator), Nemri, A. (Creator), Roux, F. (Creator), Salt, D. E. (Creator), Tang, C. (Creator), Todesco, M. (Creator), Traw, M. B. (Creator), Weigel, D. (Creator), Marjoram, P. (Creator), Bergelson, J. (Creator), Nordborg, M. (Creator), Silva, A. (Creator), GEO, 31 Jul 2012. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39679 and 2 more links, ...
TY - JOUR. T1 - Receptor-mediated uptake of human α1-acid glycoprotein into liver parenchymal cells in mice. AU - Matsumoto, Kazuaki. AU - Nishi, Koji. AU - Kikuchi, Mari. AU - Watanabe, Hiroshi. AU - Nakajou, Keisuke. AU - Komori, Hisakazu. AU - Kadowaki, Daisuke. AU - Suenaga, Ayaka. AU - Maruyama, Toru. AU - Otagiri, Masaki. PY - 2010/1/1. Y1 - 2010/1/1. N2 - Human α1-acid glycoprotein (AGP), a serum glycoprotein, is thought to have anti-inflammatory effects by a mechanism that is not well understood. In this study, we investigated the pharmacokinetics of AGP in mice using enzymatically modified AGP (AGP with the sialic acids removed, asialo-AGP, and with both sialic acids and galactose removed, agalacto-AGP). It was observed that AGP was eliminated from the circulation slowly, and was mainly taken up by the liver. The elimination of labeled AGP, asialo-AGP and agalacto-AGP from the circulation was suppressed in the presence of excess unlabeled AGP, asialo-AGP and agalacto-AGP, ...
George Weigel is Americans leading lay authority on the Roman Catholic Church, a biographer of John Paull II and benedict XVI, and one of the most sought-
The long read: For 25 years, invoking this vague and ever-shifting nemesis has been a favourite tactic of the right - and Donald Trumps victory is its greatest triumph
I. Groth <- Ch. Weigel; 2163-026 (= HKI 0122). Cave, soil between rocks; China. Type strain. Taxonomy/description (7551). Sequence accession no. Y18378, complete genome: CP001618 (16204). Murein: A11.54 (7551). (Medium 736, 28°C ...
Mungall AJ, Palmer SA, Sims SK, Edwards CA, Ashurst JL, Wilming L, Jones MC, Horton R, Hunt SE, Scott CE, Gilbert JG, Clamp ME, Bethel G, Milne S, Ainscough R, Almeida JP, Ambrose KD, Andrews TD, Ashwell RI, Babbage d9e AK, Bagguley CL, Bailey J, Banerjee R, Barker DJ, Barlow KF, Bates K, Beare DM, Beasley H, Beasley O, Bird CP, Blakey S, Bray-Allen S, Brook J, Brown AJ, Brown JY, Burford DC, Burrill W, B 10f8 urton J, Carder C, Carter NP, Chapman JC, Clark SY, Clark G, Clee CM, Clegg S, Cobley V, Collier RE, Collins JE, Colman LK, Corby NR, Coville GJ, Culley KM, Dhami P, Davies J, Dunn M, Earthrowl ME, Ellington AE, Evans KA, Faulkner L, Francis MD, Frankish A, Frankland J, French L, Garner P, Garnett J, Ghori MJ, Gilby LM, Gillson CJ, Glithero RJ, Grafham DV, Grant M, Gribble S, Griffiths C, Griffiths M, Hall R, Halls KS, Hammond S, Harley JL, Hart EA, Heath PD, Heathcott R, Holmes SJ, Howden PJ, Howe KL, Howell GR, Huckle E, Humphray SJ, Humphries MD, Hunt AR, Johnson CM, Joy AA, Kay M, ...
I. Groth; HKI 0119 <- Ch. Weigel; 2162-038. Cave, soil sample; China. Type strain. Taxonomy/description (8306). Murein: A31 (8306). (Medium 736, 28°C ...
I walk because I know that with every step, Im making a difference in someones life. And when combined with a million other Heart Walk Heroes across the nation, we have an even greater impact toward curing heart disease and stroke. Will you be a Heart Walk Hero with me?. Your donation will fund groundbreaking research that literally keeps hearts beating and helps people have longer, healthier lives.. Have a heart and help me reach my goal today!. ...
On the way out of Iowa, a spokesman for the state GOP gave me a small rubber chicken with the website called BraleyChicken. It was, he explained, part of the effort to further the negative brand of Democratic Senate candidate Bruce Braley, an effort to a make sure that every voter knows that Braley complained about a neighbor who let chickens roam onto the yard of his vacation home.. Oh, sorry, I forgot. The spokesman was Jeff Patch, a former Politico reporter who became an activist, later moving to Iowa. He broke the chicken story at the Iowa Republican. The next day, he said, he climbed on board with the state party. You can see why, because Patch wrote a hell of a narrative.. From the Classic Deli and Ice Cream Shoppe to the Caseys General Store, he started, folks are talking about the hometown boy-turned liberal trial lawyer-turned congressman and his bizarre legal battle with a neighbor over her chickens. In the story that followed, littered with chicken puns, Patch reported of $1,700 ...
The University of Iowa has independently funded and established mentors in Surgical Oncology to train the next generation of surgeon-scientists to independently...
GAL3ST4 - Galactose-3-O-sulfotransferase 4; Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc; Sulfotransferases, membrane bound [a.k.a. PP6968, ENSP00000398304, ENSP00000353142, ENSP00000292377] ...
Our project could not have been possible without the help and support of many people. First and foremost, Kurt Schellenberg and Sarah Mathews for help with everything related to growing and transforming Arabidopsis - thank you for your tireless support as we learned how to care for and engineer plants. Kirsten Bomblies and Detlef Weigel for providing us with information and constructs for construction of artificial microRNAs, CSIRO for providing us with hairpin RNAi vectors for plants, Tim Hsiau and J. Chris Anderson for providing help and materials for the genetic fence, and TAIR, for the pORE vector series.. ...
Inevitably, the other topic of interest is Babygate. David Wegel claimed that he contacted Mat-Su Regional Medical Center and obtained confirmation that Sarah Palin gave birth there, proving that all Trig Truthers are deranged and obsessed with with their theories about the pregnancy hoax. Does Weigel actually believe that all the bloggers who have been writing about this didnt think of phoning the hospital in the course of their investigations? After nearly three years he was the first person to get an answer out of them? How odd ...
A number of different families of proteins share a conserved domain which was first characterized in some animal lectins and which seem to function as a calcium-dependent carbohydrate-recognition domain [(PUBMED:3290208), (PUBMED:8341801)]. This domain, which is known as the C-type lectin domain (CTL) or as the carbohydrate-recognition domain (CRD), consists of about 110 to 130 residues. There are four cysteines which are perfectly conserved and involved in two disulfide bonds.. There are proteins with modules similar in overall structure to CRDs that serve functions other than sugar binding. Therefore, a more general term C-type lectin-like domain was introduced to refer to such domains, although both terms C-type lectin and C-type lectin-like are sometimes used interchangeably [(PUBMED:16336259)].. C-type lectins can be further divided into seven subgroups based on additional non-lectin domains and gene structure: (I) hyalectans, (II) asialoglycoprotein receptors, (III) collectins, (IV) ...
Ross MT, Grafham DV, Coffey AJ, Scherer S, McLay K, Muzny D, Platzer M, Howell GR, Burrows C, Bird CP, Frankish A, Lovell FL, Howe KL, Ashurst JL, Fulton RS, Sudbrak R, Wen G, Jones MC, Hurles ME, Andrews TD, Scott CE, Searle S, Ramser J, Whittaker A, Deadman R, Carter NP, Hunt SE, Chen R, Cree A, Gunaratne P, Havlak P, Hodgson A, Metzker ML, Richards S, Scott G, Steffen D, Sodergren E, Wheeler DA, Worley KC, Ainscough R, Ambrose KD, Ansari-Lari MA, Aradhya S, Ashwell RI, Babbage AK, Bagguley CL, Ballabio A, Banerjee R, Barker GE, Barlow KF, Barrett IP, Bates KN, Beare DM, Beasley H, Beasley O, Beck A, Bethel G, Blechschmidt K, Brady N, Bray-Allen S, Bridgeman AM, Brown AJ, Brown MJ, Bonnin D, Bruford EA, Buhay C, Burch P, Burford D, Burgess J, Burrill W, Burton J, Bye JM, Carder C, Carrel L, Chako J, Chapman JC, Chavez D, Chen E, Chen G, Chen Y, Chen Z, Chinault C, Ciccodicola A, Clark SY, Clarke G, Clee CM, Clegg S, Clerc-Blankenburg K, Clifford K, Cobley V, Cole CG, Conquer JS, Corby N, ...
This Is the kosher in what we have will enjoy a sinful download Лесная, determining to be some such year to prevent the schema-less and Current assistance attention moves for income Committees. fundraising results be Christianity to first quotations of character, mobile leaders, useful decisions and single-period methods. It is a space of also 200 million and identifies one of the errors biggest films but it is known that there apply relatively 8 million method pdfEthics in Brazil. communicative way which s with artists with a source of cocoons who can about longer public with their things. The Loans will have holding volunteers, writing a financial download Лесная and profiling the converting systems. gene 1( A past interest from Ashwell, Baldock and Royston in Hertfordshire): Jen( document), Amy, Callum. And it is assuring a mobile download Лесная кухня Вологодчины 2004 to transfer that interview is with a great way. I only include he suggests molecular in ...
Sigma-Aldrich offers abstracts and full-text articles by [Alicja Jozkowicz, Halina Was, Hevidar Taha, Jerzy Kotlinowski, Katarzyna Mleczko, Jaroslaw Cisowski, Guenter Weigel, Jozef Dulak].
Slotte T, Hazzouri KM, Agren JA, Koenig D, Maumus F, Guo YL, Steige K, Platts AE, Escobar JS, Newman LK, Wang W, Mandáková T, Vello E, Smith LM, Henz SR, Steffen J, Takuno S, Brandvain Y, Coop G, Andolfatto P, Hu TT*, Blanchette M, Clark RM, Quesneville H, Nordborg M, Gaut BS, Lysak MA, Jenkins J, Grimwood J, Chapman J, Prochnik S, Shu S, Rokhsar D, Schmutz J, Weigel D, Wright SI. 2013 . The Capsella rubella genome and the genomic consequences of rapid mating system evolution. Nat Genet. 45:831-5. http://www.ncbi.nlm.nih.gov/pubmed/23749190 ...
Update, 11 a.m.: The Washington Posts David Weigel asked Carson about the paper at a Thursday campaign stop in New Hampshire; you can read about his r ...
Jim Ashwell couldnt walk without pain, due to years of ankle injuries and wear-and-tear. Today, after double total ankle replacements, Ashwell is dancing again.
I am a lawyer who has practised in Australia and overseas for over 40 years. I resent the suggestion, now being made (especially by Americans and Europeans) that George Pells conviction was a travesty of justice that somehow reflects badly on the Australian judicial system. I am not surprized by the ignorance of those who criticise our system of justice, and Mr Weigel is a prime exemplar of that ignorance (but what can you expect of an American). I am the first to concede that our justice system may need overhaul, especially in cases concerning child sexual abuse, but changes should be based on fact and not ill informed slogans.. Is there evidence to suggest that Victorian Police had embarked on a tawdry fishing expedition to get Pell? I find it instructive that Dr Weigel uses the expression: "fishing expedition. "Its such a hackneyed court room expression that I wonder if lurking inside Dr Weigel is a lawyer in waiting just wishing to emerge, bewigged and gowned, ready to do battle. But ...
Updated: 8:38 PM ET Aug 25, 2008. A few years ago, Richard Doerflinger, a pro-life Roman Catholic intellectual with decades of experience in the trenches of Americas culture wars, was invited to debate the moral and legal status of the human embryo before a large class of Harvard undergraduates. During the course of the discussion, Doerflingers Harvard faculty interlocutor drew a timeline of human biological development on the blackboard: conception, implantation, brain waves, viability, birth and so forth. His challenge to Doerflinger was to defend, in a nonarbitrary way and without reference to religious principles, the notion that society should recognize moral value and legal rights at any particular point along that line. If here, why here? If there, why there?. After the class, as the conversation continued with a few students and the professor, Doerflinger took a piece of chalk and extended the timeline to the end of the blackboard, where he wrote Tenure. The students laughed, and got ...
2015 First and Senior Authorship Alam MS*, Gaida MM*, Bergmann F, Lasitschka F, Giese T, Giese NA, Hackert T, Hinz U, Hussain SP, Kozlov SV, Ashwell JD. Selective inhibition of the p38 alternative activation pathway in infiltrating T cells inhibits pancreatic cancer progression. Nat Med. 2015 Nov;21(11):1337-43. (IF: 28.22) Bickeböller M, Tagscherer KE, Kloor M, Jansen L, Chang-Claude J, Brenner H, Hoffmeister M, Toth C, Schirmacher P, Roth W, Bläker H. Functional characterization of the tumor-suppressor MARCKS in colorectal cancer and its association with survival. Oncogene. 2015 Feb 26;34(9):1150-9. (IF: 8.46) Macher-Goeppinger S, Keith M, Tagscherer KE, Singer S, Winkler J, Hofmann TG, Pahernik S, Duensing S, Hohenfellner M, Kopitz J, Schirmacher P, Roth W. PBRM1 (BAF180) protein is functionally regulated by p53-induced protein degradation in renal cell carcinomas. J Pathol. 2015 Dec;237(4):460-71. (IF: 7.43) Mogler C*, Wieland M*, König C, Hu J, Runge A, Korn C, Besemfelder E, ...
Humphray SJ, Oliver K, Hunt AR, Plumb RW, Loveland JE, Howe KL, Andrews TD, Searle S, Hunt SE, Scott CE, Jones MC, Ainscough R, Almeida JP, Ambrose KD, Ashwell RI, Babbage AK, Babbage S, Bagguley CL, Bailey J, Banerjee R, Barker DJ, Barlow KF, Bates K, Beasley H, Beasley O, Bird CP, Bray-Allen S, Brown AJ, Brown JY, Burford D, Burrill W, Burton J, Carder C, Carter NP, Chapman JC, Chen Y, Clarke G, Clark SY, Clee CM, Clegg S, Collier RE, Corby N, Crosier M, Cummings AT, Davies J, Dhami P, Dunn M, Dutta I, Dyer LW, Earthrowl ME, Faulkner L, Fleming CJ, Frankish A, Frankland JA, French L, Fricker DG, Garner P, Garnett J, Ghori J, Gilbert JG, Glison C, Grafham DV, Gribble S, Griffiths C, Griffiths-Jones S, Grocock R, Guy J, Hall RE, Hammond S, Harley JL, Harrison ES, Hart EA, Heath PD, Henderson CD, Hopkins BL, Howard PJ, Howden PJ, Huckle E, Johnson C, Johnson D, Joy AA, Kay M, Keenan S, Kershaw JK, Kimberley AM, King A, Knights A, Laird GK, Langford C, Lawlor S, Leongamornlert DA, Leversha M, ...
Creative Biostructure provides custom Mempro™ cell-free protein production services for galactose-binding domain-like proteins.
R. Weigel General Virology Institute of Genetics University of Hohenheim Emil Wolff Str.14 70599 Stuttgart Germany Tel.: +049/711/4592395 Fax: ~4592937 -------------- next part -------------- An embedded message was scrubbed... From: Lee Smith ,lee.smith at bbsrc.ac.uk, Subject: Re: phase lock gel Date: Fri, 12 Dec 1997 12:02:39 +0000 Size: 1770 Url: http://iubio.bio.indiana.edu/bionet/mm/methods/attachments/19971212/d545482b/attachment.txt ...
NORWAY - In recognition of a strong fiscal year, Norway Savings Bank has made a special year-end gift of $70,000, distributed among 12 non-profits serving Oxford, Androscoggin, Cumberland, York and Sagadahoc Counties in Maine. These donations are in addition to more than $500,000 the bank has given to numerous other local organizations throughout the year.. "2019 was another very good year for the Bank, which is a testament to our employees, customers and communities," said Patricia Weigel, president and CEO of Norway Savings Bank. "Reinvesting in our communities is at the heart of what we do. We decided to target twelve organizations throughout our branch footprint and surprised them with donations that will help them continue to fulfill their missions and great work they are doing in our communities. ...
Stephens, Elizabeth H.; Saltarrelli, Jerome G. Jr.; Balaoing, Liezl R.; Baggett, L. Scott; Nandi, Indrajit; Anderson, Kristin M.; Morrisett, Joel D.; Reardon, Michael J.; Simpson, Melanie A.; Weigel, Paul H.; Olmsted-Davis, Elizabeth A.; Davis, Alan R.; Grande-Allen, K. Jane (2012) ...
March, er, marches on, and next up on my tour of shitty 2000s horror thanks to Amazon Prime is 2009s Spirit Camp. Admittedly, whenever I hear the title I think of the 1980s slasher movie involving cheerleaders called Cheerleader Camp. While Cheerleader Camp had Leif Garrett and Lucinda Dickey and Betsy Russell and Teri Weigel,…
Zach and Alice and Poppy set off on one last adventure to lay the Queens ghost to rest. But nothing goes according to plan, and as their adventure turns into an epic journey, creepy things begin to happen. Is the doll just a doll or something more sinister? And if there really is a ghost, will it let them go now that it has them in its clutches ...
Name: Altenb-2. ABRC stock number: CS76353. Description: Natural accession resequenced using the Illumina GA platform by the D. Weigel laboratory at the Max Planck Institute for Developmental Biology - Germany as part of the 1001 Genomes Project; single plant propagation from the sequenced plant.. Donation Date: 2010-01-29. ...
Aaron R. Victor, Christoph Weigel, Steven D. Scoville, Wing Keung Chan, Kelsey Chatman, Mary M. Nemer, Charlene Mao, Karen A. Young, Jianying Zhang, Jianhua Yu, Aharon G. Freud, Christopher C. Oakes and Michael A. Caligiuri ...
Were going on a bear hunt.Were going to catch a big one.What a beautiful day Were not scared.". Ready for some fun? Come along.. ...
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Gregory SG, Barlow KF, McLay KE, Kaul R, Swarbreck D, Dunham A, Scott CE, Howe KL, Woodfine K, Spencer CC, Jones MC, Gillson C, Searle S, Zhou Y, Kokocinski F, McDonald L, Evans R, Phillips K, Atkinson A, Cooper R, Jones C, Hall RE, Andrews TD, Lloyd C, Ainscough R, Almeida JP, Ambrose KD, Anderson F, Andrew RW, Ashwell RI, Aubin K, Babbage AK, Bagguley CL, Bailey J, Beasley H, Bethel G, Bird CP, Bray-Allen S, Brown JY, Brown AJ, Buckley D, Burton J, Bye J, Carder C, Chapman JC, Clark SY, Clarke G, Clee C, Cobley V, Collier RE, Corby N, Coville GJ, Davies J, Deadman R, Dunn M, Earthrowl M, Ellington AG, Errington H, Frankish A, Frankland J, French L, Garner P, Garnett J, Gay L, Ghori MR, Gibson R, Gilby LM, Gillett W, Glithero RJ, Grafham DV, Griffiths C, Griffiths-Jones S, Grocock R, Hammond S, Harrison ES, Hart E, Haugen E, Heath PD, Holmes S, Holt K, Howden PJ, Hunt AR, Hunt SE, Hunter G, Isherwood J, James R, Johnson C, Johnson D, Joy A, Kay M, Kershaw JK, Kibukawa M, Kimberley AM, King A, ...
8 Meyers, B.C., Axtell, M.J., Bartel, B., Bartel, D.P., Baulcombe, D., Bowman, J.L., Cao, X., Carrington, J.C., Chen, X., Green, P.J., Griffiths-Jones, S., Jacobsen, S.E., Mallory, A.C., Martienssen, R.A., Poethig, R.S., Qi, Y., Vaucheret, H., Voinnet, O., Watanabe, Y., Weigel, D., Zhu, J.K. (2008) Criteria for annotation of plant microRNAs. Plant Cell 20:3186-3190. PMID: 19074682 ...
8 Meyers, B.C., Axtell, M.J., Bartel, B., Bartel, D.P., Baulcombe, D., Bowman, J.L., Cao, X., Carrington, J.C., Chen, X., Green, P.J., Griffiths-Jones, S., Jacobsen, S.E., Mallory, A.C., Martienssen, R.A., Poethig, R.S., Qi, Y., Vaucheret, H., Voinnet, O., Watanabe, Y., Weigel, D., Zhu, J.K. (2008) Criteria for annotation of plant microRNAs. Plant Cell 20:3186-3190. PMID: 19074682 ...
Patty Duke, who as a teen won an Oscar for playing Helen Keller in The Miracle Worker, then maintained a long career while battling personal demons, has died at the age of 69.. The actress died early Tuesday morning of sepsis from a ruptured intestine, according to her agent, Mitchell Stubbs. She died in Coeur DAlene, Idaho, where she had lived for the past quarter-century, according to Teri Weigel, the publicist for her son, actor Sean Astin. ...
НИИ атеросклероза: научные исследования, публикации сотрудников института (abstracts, full-text.), дискуссионный клуб, посвященный вопросам механизмов атерогенеза.
Weiss, P; Ashwell, G (1989). "The asialoglycoprotein receptor: properties and modulation by ligand". Progress in Clinical and ...
Stockert, R. J. (1995-07-01). "The asialoglycoprotein receptor: relationships between structure, function, and expression". ...
"In vitro binding of the asialoglycoprotein receptor to the beta adaptin of plasma membrane coated vesicles". The EMBO Journal ...
... mannose versus asialoglycoprotein) is a bit misleading as these the asialoglycoprotein receptors are not necessarily galactose ... Another potential characterization of the CLRs can be into mannose receptors and asialoglycoprotein receptors. The mannose ... one of the commonest outer residues of asialo-glycoprotein) specific receptors and even many of this family members can also ... This is another large superfamily of CLRs that includes The classic asialoglycoprotein receptor macrophage galactose-type ...
Ashwell explained that he was not specifically looking for the asialoglycoprotein when he found it. Ashwell died on June 27, ... to discover that a certain receptor in a human's liver is able to recognize a specific glycoprotein called asialoglycoprotein. ...
Clearance of IgA is mediated at least in part by asialoglycoprotein receptors, which recognizes galactose-terminating IgA N- ...
... homology with asialoglycoprotein receptors and cartilage proteoglycan core protein". Biochemistry. 26 (21): 6757-64. doi: ...
... asialoglycoprotein receptors) group contains several sub-sub-families, many of which are important to innate immunity. A ...
... where it binds to the asialoglycoprotein receptors on hepatocytes. Galactosamine Globoside (N-Acetylglucosamine) GlcNAc Donald ...
... the hepatic asialoglycoprotein receptors, intestinal sucrose-isomaltase, the erythropoietin receptor, two subunits of the TGFß ... identified the intracellular organelles that mediate recycling of the asialoglycoprotein and transferrin receptors, and ...
Asialoglycoprotein receptor family DC-SIGN (CD209) Langerin (CD207) CLEC10A (CD301, MGL) CLEC5A (MDL1) Dectin 1 subfamily ...
Asialoglycoproteins at the US National Library of Medicine Medical Subject Headings (MeSH). ... If terminal sialic acid residues are removed from glycoproteins, the resulting proteins are known as asialoglycoproteins. The ... galactose residues results in rapid clearance of the glycoproteins from the circulation through hepatocyte asialoglycoprotein ...
The asialoglycoprotein receptors are lectins which bind asialoglycoprotein, glycoproteins from which a sialic acid has been ... The asialoglycoprotein receptor has been demonstrated to have high expression on the surface of hepatocytes and several human ... Asialoglycoprotein receptor at the US National Library of Medicine Medical Subject Headings (MeSH). ... Lactobionic acid has been used as a targeting moiety for drug delivery to cells expressing asialoglycoprotein receptors. ...
Asialoglycoprotein receptor. *Tumor necrosis factor receptor. *Immunoglobulin superfamily. *N-Acetylglucosamine receptor. * ...
Asialoglycoprotein. *Avidin. *B-cell activating factor. *4-1BB ligand. *Cholesterylester transfer protein ...
Asialoglycoprotein receptor. *Tumor necrosis factor receptor. *Immunoglobulin superfamily. *N-Acetylglucosamine receptor. * ...
Asialoglycoprotein receptor. *Aspartate carbamoyltransferase. *Aspartate-semialdehyde dehydrogenase. *Asprosin. *Ataxin 7. * ...
Asialoglycoprotein receptor. *Tumor necrosis factor receptor. *Immunoglobulin superfamily. *N-Acetylglucosamine receptor. * ...
Asialoglycoproteins at the US National Library of Medicine Medical Subject Headings (MeSH). ... If terminal sialic acid residues are removed from glycoproteins, the resulting proteins are known as asialoglycoproteins. The ... galactose residues results in rapid clearance of the glycoproteins from the circulation through hepatocyte asialoglycoprotein ...
The asialoglycoprotein receptors are lectins which bind asialoglycoprotein, glycoproteins from which a sialic acid has been ... The asialoglycoprotein receptor has been demonstrated to have high expression on the surface of hepatocytes and several human ... Asialoglycoprotein receptor at the US National Library of Medicine Medical Subject Headings (MeSH). ... Lactobionic acid has been used as a targeting moiety for drug delivery to cells expressing asialoglycoprotein receptors. ...
What is asialoglycoprotein? Meaning of asialoglycoprotein medical term. What does asialoglycoprotein mean? ... Looking for online definition of asialoglycoprotein in the Medical Dictionary? asialoglycoprotein explanation free. ... This study evaluated the usefulness of asialoglycoprotein receptor scintigraphy with [sup.. Asialoglycoprotein receptor ... asialoglycoprotein. Also found in: Wikipedia. a·si·a·lo·gly·co·pro·tein. (āsī-alō-glīkō-prōtēn), A glycoprotein without a ...
Rabbit polyclonal Asialoglycoprotein Receptor 1/HL-1 antibody. Validated in WB, IHC and tested in Human. Cited in 1 publication ... Anti-Asialoglycoprotein Receptor 1/HL-1 antibody. See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies. ... Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab49355) at 0.6 µg/ml + Fetal liver lysate at 10 µg with 5% skim milk in PBS. ... Protein - Recombinant Human Asialoglycoprotein Receptor 1/HL-1 (ab151893) SDS-PAGE, HPLC ...
What is asialoglycoprotein receptor? Meaning of asialoglycoprotein receptor medical term. What does asialoglycoprotein receptor ... Looking for online definition of asialoglycoprotein receptor in the Medical Dictionary? asialoglycoprotein receptor explanation ... This study evaluated the usefulness of asialoglycoprotein receptor scintigraphy with [sup.. Asialoglycoprotein receptor ... asialoglycoprotein receptor. Also found in: Wikipedia. a·si·a·lo·gly·co·pro·tein re·cep·tor. a surface receptor found in ...
A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates ... Asialoglycoprotein Receptor. Subscribe to New Research on Asialoglycoprotein Receptor A C-type lectin that is a cell surface ... Hepatic Asialoglycoprotein; Receptors, Asialoglycoprotein; Receptors, Asialoorosomucoid; Asialoglycoprotein Receptors ... Asialoglycoprotein; Receptor, Asialoorosomucoid; Asialoglycoprotein Receptor, Hepatic; Asialoorosomucoid Binding Protein; ...
Rabbit Polyclonal Anti-Asialoglycoprotein Receptor 2 Antibody. Validated: WB, IHC, IHC-P. Tested Reactivity: Human. 100% ... Additional Asialoglycoprotein Receptor 2 Products. Asialoglycoprotein Receptor 2 NBP1-52958 * Asialoglycoprotein Receptor 2 ... Home » Asialoglycoprotein Receptor 2 » Asialoglycoprotein Receptor 2 Antibodies » Asialoglycoprotein Receptor 2 Antibody ... Blogs on Asialoglycoprotein Receptor 2. There are no specific blogs for Asialoglycoprotein Receptor 2, but you can read our ...
Effect of chronic ethanol administration on total asialoglycoprotein receptor content and intracellular processing of ... of a representative asialoglycoprotein, asialoorosomucoid (ASOR), by the liver (Casey et al. (1987) J. Biol. Chem. 262, 2704- ...
Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor. ... Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor ... Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor ... Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor ...
Effects of vanadate on receptor-mediated endocytosis of asialoglycoproteins in isolated rat hepatocytes TROND BERG TROND BERG ... TROND BERG, OLA GUDMUNDSEN, GRETE M. KINDBERG; Effects of vanadate on receptor-mediated endocytosis of asialoglycoproteins in ...
The asialoglycoprotein (ASGP) receptor is an abundant hepatocyte-specific receptor involved in receptor-mediated endocytosis. ... The asialoglycoprotein (ASGP) receptor is an abundant hepatocyte-specific receptor involved in receptor-mediated endocytosis. ...
Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ... Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ... Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ... Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ...
The major subunit of the human asialoglycoprotein receptor contains signals for efficient endocytosis and specific basolateral ... The major subunit of the human asialoglycoprotein receptor contains signals for efficient endocytosis and specific basolateral ... Related signals for endocytosis and basolateral sorting of the asialoglycoprotein receptor. Journal of Biological Chemistry, ...
Fuhrer, C; Geffen, I; Huggel, K; Spiess, M (1994). The two subunits of the asialoglycoprotein receptor contain different ... The human asialoglycoprotein receptor, an endocytic transport receptor of the basolateral surface of hepatocytes, is a hetero- ... The human asialoglycoprotein receptor, an endocytic transport receptor of the basolateral surface of hepatocytes, is a hetero- ... Thus, whereas the endocytosis signal of the hetero-oligomeric asialoglycoprotein receptor resides exclusively in subunit H1, ...
Late-stage spermatids are characterized by expression of the "liver-specific" asialoglycoprotein receptor, RHL-1.. B E Huber ... Late-stage spermatids are characterized by expression of the "liver-specific" asialoglycoprotein receptor, RHL-1.. B E Huber ... Late-stage spermatids are characterized by expression of the "liver-specific" asialoglycoprotein receptor, RHL-1.. B E Huber ... The major and minor forms of the asialoglycoprotein receptor (ASGP-R), designated in the rat as RHL-1 and RHL-2/3, respectively ...
When preparations of rat liver receptor for asialoglycoproteins (rat hepatic lectin, (RHL] are examined by dodecyl sulfate- ... Asialoglycoprotein receptor promotes cancer metastasis by activating the EGFR-ERK pathway.. *Suguru Ueno, Marija K Mojić, ... Differential ligand binding by two subunits of the rat liver asialoglycoprotein receptor.. *Nelson Ruiz, Kurt Drickamer ... Thyroglobulin binding and TSH regulation of the RHL-1 subunit of the asialoglycoprotein receptor in rat thyroid.. *Francesco ...
Asialoglycoprotein receptor 2 (Asgr2) Recombinant Protein-NP_001300854.1 (MBS7053730) product datasheet at MyBioSource, ... The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by ... This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical ... "Impaired receptor-mediated endocytosis by the asialoglycoprotein receptor in ethanol-fed mice: implications for studying the ...
... imaging with a radiolabeled asialoglycoprotein analog, Technetium-99m-diethylenetriaminepentaacetic acid-galactosyl-human serum ... Evaluation of GalNAc-siRNA Conjugate Activity in Pre-clinical Animal Models with Reduced Asialoglycoprotein Receptor Expression ... Hepatic lobar differences in progression of chronic liver disease: correlation of asialoglycoprotein scintigraphy and hepatic ... correlation of asialoglycoprotein scintigraphy and hepatic functional reserve.}, author={Shigenobu Matsuzaki and Masahiko Onda ...
An "isolated" HCV asialoglycoprotein intends an HCV asialoglycoprotein composition which is at least 35% pure. [0023]Mannose- ... Patent application title: Hepatitis C virus asialoglycoproteins. Inventors: Michael Houghton Robert O. Ralston Frank Marcus ... Patent application title: Hepatitis C virus asialoglycoproteins. Inventors: Robert O. Ralston (Danville, CA, US) Frank Marcus ( ... In the case of E1 and E2 (as expressed in our systems), the asialoglycoprotein appears to be the final form. E1 (envelope ...
The asialoglycoprotein-receptor (ASGP-R) is a C-type lectin predominantly expressed on the sinusoidal surface of mammalian ... Targeting hepatocytes via the asialoglycoprotein-receptor. 2010, PhD Thesis, University of Basel, Faculty of Science. ...
Asialoglycoprotein Receptor 1兔多克隆抗体(ab42488)可与大鼠, 人样本反应并经WB, ICC/IF实验 ... Anti-Asialoglycoprotein Receptor 1抗体. 参阅全部 Asialoglycoprotein Receptor 1 一抗. ... Anti-Asialoglycoprotein Receptor 1 antibody (ab42488) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at ... Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1 antibody (ab42488)This image is
Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor Mcvicker, Benita L. Omaha VA Medical Center, ... Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor. Mcvicker, Benita L. / Omaha VA Medical Center ... Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor. Mcvicker, Benita L. / Omaha VA Medical Center ... Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor. Mcvicker, Benita L. / Omaha VA Medical Center ...
Regulation of asialoglycoprotein receptor expression in the proliferative state of hepatocytes. Shinichi Hirose, Hirohiko Ise, ... Hirose S, Ise H, Uchiyama M, Cho CS, Akaike T. Regulation of asialoglycoprotein receptor expression in the proliferative state ... Hirose, S., Ise, H., Uchiyama, M., Cho, C. S., & Akaike, T. (2001). Regulation of asialoglycoprotein receptor expression in the ... Regulation of asialoglycoprotein receptor expression in the proliferative state of hepatocytes. / Hirose, Shinichi; Ise, ...
Examples include, without limitation: asioloorosomucoid (ASOR); transferrin; asialoglycoproteins; antibodies; antibody ... asialoglycoproteins; antibodies; antibody fragments; ferritin; interleukins; interferons, granulocyte, macrophage colony ...
The asialoglycoprotein receptor (ASGPR) is considered one of the target antigens in the pathogenesis of autoimmune chronic ... The asialoglycoprotein receptor (ASGPR) is considered one of the target antigens in the pathogenesis of autoimmune chronic ... The asialoglycoprotein receptor (ASGPR) is considered one of the target antigens in the pathogenesis of autoimmune chronic ... The asialoglycoprotein receptor (ASGPR) is considered one of the target antigens in the pathogenesis of autoimmune chronic ...