Endogenous glycoproteins from which SIALIC ACID has been removed by the action of sialidases. They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by adsorptive ENDOCYTOSIS they are delivered to LYSOSOMES for degradation. Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. They are elevated in serum of patients with HEPATIC CIRRHOSIS or HEPATITIS.
A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates the endocytosis of serum glycoproteins.
A family of calcium-binding alpha-globulins that are synthesized in the LIVER and play an essential role in maintaining the solubility of CALCIUM in the BLOOD. In addition the fetuins contain aminoterminal cystatin domains and are classified as type 3 cystatins.
An enzyme that oxidizes galactose in the presence of molecular oxygen to D-galacto-hexodialdose. It is a copper protein. EC 1.1.3.9.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Cell surface molecules on cells of the immune system that specifically bind surface molecules or messenger molecules and trigger changes in the behavior of cells. Although these receptors were first identified in the immune system, many have important functions elsewhere.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
The rate dynamics in chemical or physical systems.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
Systems in which an intracellular signal is generated in response to an intercellular primary messenger such as a hormone or neurotransmitter. They are intermediate signals in cellular processes such as metabolism, secretion, contraction, phototransduction, and cell growth. Examples of second messenger systems are the adenyl cyclase-cyclic AMP system, the phosphatidylinositol diphosphate-inositol triphosphate system, and the cyclic GMP system.
Male germ cells derived from the haploid secondary SPERMATOCYTES. Without further division, spermatids undergo structural changes and give rise to SPERMATOZOA.
The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.
The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA.
Exclusive legal rights or privileges applied to inventions, plants, etc.
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.
A specific mannose-binding member of the collectin family of lectins. It binds to carbohydrate groups on invading pathogens and plays a key role in the MANNOSE-BINDING LECTIN COMPLEMENT PATHWAY.
A subclass of lectins that are specific for CARBOHYDRATES that contain MANNOSE.
A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.

Stabilization of poly-L-lysine/DNA polyplexes for in vivo gene delivery to the liver. (1/431)

We are developing a self-assembling non-viral in vivo gene delivery vehicle based on poly-l-lysine and plasmid DNA. We have characterized poly-l-lysines of different chain lengths for DNA condensation and strength of DNA binding. Poly-l-lysine chains >20 residues bound DNA efficiently in physiological saline, while shorter chains did not. Attachment of asialoorosomucoid to PLL increased the PLL chain length required for efficient DNA binding in saline and for efficient DNA condensation. By electron microscopy, poly-l-lysine/DNA polyplexes appeared as toroids 25-50 nm in diameter or rods 40-80 nm long; conjugation of asialoorosomucoid to the polylysine component increased the size of resulting polyplexes to 50-90 nm. In water, poly-l-lysine and asialoorosomucoid-PLL polyplexes have effective diameters of 46 and 87.6 nm, respectively. Polyplexes containing only poly-l-lysine and DNA aggregated in physiological saline at all charge ratios and aggregated at neutral charge ratios in water. Attachment of asialoorosomucoid lessened, but did not eliminate, the aggregation of PLL polyplexes, and did not result in efficient delivery of polyplexes to hepatocytes. Conjugation of polyethylene glycol to poly-l-lysine sterically stabilized resulting polyplexes at neutral charge ratios by shielding the surfaces. For efficient in vivo gene delivery, polyplexes will need to be sterically stabilized to prevent aggregation and interaction with serum components.  (+info)

Nucleotide exchange in genomic DNA of rat hepatocytes using RNA/DNA oligonucleotides. Targeted delivery of liposomes and polyethyleneimine to the asialoglycoprotein receptor. (2/431)

Chimeric RNA/DNA oligonucleotides have been shown to promote single nucleotide exchange in genomic DNA. A chimeric molecule was designed to introduce an A to C nucleotide conversion at the Ser365 position of the rat factor IX gene. The oligonucleotides were encapsulated in positive, neutral, and negatively charged liposomes containing galactocerebroside or complexed with lactosylated polyethyleneimine. The formulations were evaluated for stability and efficiency in targeting hepatocytes via the asialoglycoprotein receptor. Physical characterization and electron microscopy revealed that the oligonucleotides were efficiently encapsulated within the liposomes, with the positive and negative formulations remaining stable for at least 1 month. Transfection efficiencies in isolated rat hepatocytes approached 100% with each of the formulations. However, the negative liposomes and 25-kDa lactosylated polyethyleneimine provided the most intense nuclear fluorescence with the fluorescein-labeled oligonucleotides. The lactosylated polyethyleneimine and the three different liposomal formulations resulted in A to C conversion efficiencies of 19-24%. In addition, lactosylated polyethyleneimine was also highly effective in transfecting plasmid DNA into isolated hepatocytes. The results suggest that both the liposomal and polyethyleneimine formulations are simple to prepare and stable and give reliable, reproducible results. They provide efficient delivery systems to hepatocytes for the introduction or repair of genetic mutations by the chimeric RNA/DNA oligonucleotides.  (+info)

Copper and zinc ions differentially block asialoglycoprotein receptor-mediated endocytosis in isolated rat hepatocytes. (3/431)

Asialoglycoprotein receptors on hepatocytes lose endocytic and ligand binding activity when hepatocytes are exposed to iron ions. Here, we report the effects of zinc and copper ions on the endocytic and ligand binding activity of asialoglycoprotein receptors on isolated rat hepatocytes. Treatment of cells at 37 degrees C for 2 h with ZnCl2 (0-220 microM) or CuCl2 (0-225 microM) reversibly blocked sustained endocytosis of 125I-asialoorosomucoid by up to 93% (t1/2 = 62 min) and 99% (t1/2 = 54 min), respectively. Cells remained viable during such treatments. Zinc- and copper-treated cells lost approximately 50% of their surface asialoglycoprotein receptor ligand binding activity; zinc-treated cells accumulated inactive asialoglycoprotein receptors intracellularly, whereas copper-treated cells accumulated inactive receptors on their surfaces. Cells treated at 4 degrees C with metal did not lose surface asialoglycoprotein receptor activity. Exposure of cells to copper ions, but not to zinc ions, blocked internalization of prebound 125I-asialoorosomucoid, but degradation of internalized ligand and pinocytosis of the fluid-phase marker Lucifer Yellow were not blocked by metal treatment. Zinc ions reduced diferric transferrin binding and endocytosis on hepatocytes by approximately 33%; copper ions had no inhibitory effects. These findings are the first demonstration of a specific inhibition of receptor-mediated endocytosis by non-iron transition metals.  (+info)

Asialoglycoprotein receptor scintigraphy in evaluation of auxiliary partial orthotopic liver transplantation. (4/431)

The purpose of this study was to evaluate asialoglycoprotein receptor scintigraphy in the post-transplant monitoring of liver graft and native liver functions in recipients of auxiliary partial orthotopic liver transplantation (APOLT) from living donors. METHODS: We performed 36 asialoglycoprotein receptor scintigraphies on 13 patients who had undergone APOLT for noncirrhotic metabolic liver diseases or for small-for-size grafts. The portal vein of the native liver was separated in 12 patients. Anterior dynamic images including the heart and both livers were obtained for 16 min after intravenous injection of 99mTc-diethylenetriamine pentaacetic acid-galactosyl human serum albumin (GSA), and thereafter static SPECT images of both livers were obtained. Uptake rates from the blood to the graft and to the native liver were determined separately by Patlak plot graphical analysis. Relative uptake of GSA by the graft was calculated from transverse SPECT images. The relative volume of the graft liver was determined by CT. RESULTS: The relative uptake of GSA by the graft was higher or increased more rapidly than the relative volume of the graft in 8 of 11 patients with no severe complications concerning the graft. The relative uptake by severely damaged graft liver in 2 patients was much lower than the relative volume. The uptake rate of GSA by the graft was low in these 2 patients. The uptake rate by the native liver decreased when the portal vein was separated. CONCLUSION: The relative uptake of GSA was a better indicator of graft liver function than was anatomic volume. The uptake rate provided additional independent information of each liver. Asialoglycoprotein receptor scintigraphy is useful for distinguishing and monitoring the graft and native liver functions in patients who had undergone APOLT.  (+info)

Molecular cloning and expression of Galbeta1,3GalNAc alpha2, 3-sialyltransferase from human fetal liver. (5/431)

Based on the sequences of the highly conserved segments in the previously cloned sialyltransferases, a cDNA encoding Galbeta1, 3GalNAc alpha2,3-sialyltransferase (SIATFL) has been isolated from human fetal liver. Expression analysis of the gene has been performed with various carcinoma cell lines, fetal tissues, fetal and adult liver and both hepatoma and the surrounding tissue from the same liver. The SIATFL gene was expressed poorly in fetal liver and in adult liver, slightly in hepatoma and highly in the surrounding tissue of hepatoma. The cDNA encoding the putative active domain was expressed in COS-1, Escherichia coli, and Pichia pastoris. The recombinant protein expressed in COS-1 could catalyse the transfer of NeuAc from CMP-NeuAc to asialo-fetuin. No enzyme activity was detected with a 32-kDa protein in E. coli and both 32-kDa and 41-kDa proteins in P. pastoris. These results suggested that correct glycosylation of the enzyme might play a key role in its folding that may be directly related to the enzymatic activity.  (+info)

Characterization of recombinant and plant-derived mistletoe lectin and their B-chains. (6/431)

Mistletoe lectin I (pML) and its isoforms ML II and III constitute the active principle in extract preparations from mistletoe, commonly used as immunomodulator in adjuvant tumour therapy. The heterodimeric disulfide-linked cytotoxic protein is classified as type II ribosome inactivating protein (RIP). Recently, the sequence coding for the mistletoe lectin prepro-protein was identified and the existence of a single intron-free gene was shown [Eck, J., Langer, M., Mockel, B., Baur, A., Rothe, M., Zinke, H. & Lentzen, H. (1999) Eur. J. Biochem. 264, 775-784]. The aim of this study was to prepare pure and homogeneous rMLB-chain as well as rML heterodimer for studying the carbohydrate binding specificity of recombinant versus natural protein and its contribution to the observed cytotoxic effect. Expression in E. coli resulted in the production of insoluble protein (inclusion bodies). A procedure for generating correctly folded, biochemically and biologically active rMLB was established starting from the insoluble single chain. Carbohydrate binding and specificity of pMLB and rMLB were analysed by a competitive enzyme linked lectin assay (ELLA). Asialofetuin was able to compete with binding of both chains (50% at 0.8 microM). The specificity of the B-chains to lactose was more distinct with halfmaximal competition at 4.9 mM (pMLB) and > 90 mM (rMLB), respectively. Furthermore, in a coassociation process rMLA- and rMLB inclusion bodies were associated in one step by defined dilution yielding active rML-heterodimer. The activities of recombinant (rML) and plant derived mistletoe lectin (pML) were compared. Cytotoxicity was determined using MOLT-4 cells and enzymatic rRNA N-glycosidase activity was measured in a coupled transcription/translation assay. The IC50 values of the two heterodimers were similar in both assays; rMLB-chain did not show any cytotoxic effect. In the ELLA with lactose as a competitor 50% competition of binding to asialofetuin was achieved at 1.6 mM (rML) and 1.8 mM (pML). Hence, using three different assays we found no significant differences between the recombinant protein and the glycosylated form of ML. Comparing the biological activities of the single chains with those of the heterodimer we conclude, that both, lectin activity and the rRNA N-glycosidase activity, are prerequisites for the cytotoxic effects on target cells.  (+info)

Evaluation of the components of the chylomicron remnant removal mechanism by use of the isolated perfused mouse liver. (7/431)

The isolated perfused mouse liver was utilized to evaluate the relative contribution of various molecules believed to participate in the removal of chylomicron remnants by the liver. Sixty percent of asialofetuin was removed from the perfusate per pass; bovine serum albumin was not removed. Normal mouse livers removed chylomicron remnants more efficiently (40-50%/pass) than nascent chylomicrons (10-20%/pass). The fractional removal rate of remnants decreased as their concentration in the perfusate increased demonstrating saturability. Remnant removal by livers of low density lipoprotein receptor-deficient (LDLRD) mice paralleled that of normal mice at low remnant concentrations (0.05, 0.2 microg protein/ml); as concentration increased (4-16 microg protein/ml), removal by LDLRD livers was reduced. About 50% of the capacity to remove remnants was due to the LDL receptor. The role of the LDLR-related protein (LRP) was estimated using the receptor-associated protein (RAP). Four microg/ml of RAP inhibited only LRP; it reduced the removal of remnants by 30-40% in normal livers. When RAP was included in the perfusate of LDLRD livers, remnant removal persisted but was diminished, particularly late in the perfusion; the capacity was approximately 30% of controls. The present study has established that there is more than one mechanism operating for the removal of chylomicron remnants by the liver, provides estimates of the concentration of each to the removal of remnants, and indicates a method for further studies. It is concluded that in normal livers, the LDL receptor has the greatest capacity for removing chylomicron remnants. The LRP contributes to the process as well and a third component, perhaps "sequestration," accounts for up to 30% of the capacity for the initial removal of chylomicron remnants.  (+info)

Fluid phase endocytosis and galactosyl receptor-mediated endocytosis employ different early endosomes. (8/431)

Endocytosis may originate both in coated pits and in uncoated regions of the plasma membrane. In hepatocytes it has been shown that fluid phase endocytosis (here defined as 'pinocytosis') is unaffected by treatments that arrest coated pit-mediated endocytosis, indicating that pinocytosis is primarily a clathrin-independent process. In this study we have tried to determine possible connections between pinocytosis and clathrin-dependent endocytosis in rat hepatocytes by means of subcellular fractionation, electron microscopy, and by assessing the influence of inhibitors of clathrin-dependent endocytosis on pinocytosis. As marker for clathrin-dependent endocytosis was used asialoorosomucoid (AOM) labelled with [(125)I]tyramine cellobiose ([(125)I]TC). [(125)I]TC-labelled bovine serum albumin ([(125)I]TC-BSA) was found to be a useful marker for pinocytosis. Its uptake in the cells is not saturable, and any remnants of [(125)I]TC-BSA associated with the cell surface could be removed by incubating the cells with 0.3% pronase at 0 degrees C for 60 min. The data obtained by electron microscopy and by subcellular fractionation suggested that early after initiation of uptake (<15 min) [(125)I]TC-BSA and [(125)I]TC-AOM were present in different endocytic vesicles. The two probes probably join prior to their entrance in the lysosomal compartment. The relation between endocytosis via coated pits and pinocytosis was also studied with techniques that induced a selective density shift either in the clathrin-dependent pathway (by AOM-HRP) or in the pinocytic pathway (by allowing uptake of AuBSA). Both treatments indicated that the two probes ([(125)I]TC-AOM and [(125)I]TC-BSA) were early after uptake, at least partly, in separate endocytic compartments. The different distribution of the fluid phase marker and the ligand (internalised via coated pits) was not due to a difference in the rate at which they enter a later compartment, since a lowering of the incubation temperature to 18 degrees C, which should keep the probes in the early endosomes, did not affect their early density distribution. Incubation of cells in a hypertonic medium reduced uptake both of [(125)I]TC-AOM and [(125)I]TC-BSA; the uptake of [(125)I]TC-AOM was, however, reduced much more than that of the fluid phase marker. This finding supports the notion that the two probes enter the cells via different routes.  (+info)

The ability of a peptide vaccine to stimulate T cells in vivo might be improved by specifically targeting the peptide to dendritic cells (DC). The C-type lectin Macrophage Galactose-type lectin, MGL (CD301), has been shown to bind to N-acetyl-galactosamine (GalNAc) and small peptides bearing O-linked GalNAc. Synthetic GalNAc can be produced using relatively simple organic chemistry when compared with the complicated branched sugars that are recognised by other C-type lectins. MGL therefore represents a promising target for the design of synthetic peptide vaccines. We have identified that antigen-presenting cells in human skin express MGL and have confirmed that CD14+ dermal DCs might be targeted via MGL. The intracellular fate of MGL following internalisation was tracked by confocal microscopy. MGL traffics through early endosomes to late endosomes/lysosomes, and colocalises with MHC class I and class II. Synthetic glycopeptides were produced incorporating either native O-linked GalNAc or GalNAc ...
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IFDB is a game catalog and recommendation engine for Interactive Fiction, also known as Text Adventures. IFDB is a collaborative, wiki-style community project. Members can contribute game listings, reviews, recommendations, and more.
Xiu-Sheng Miao, Pengfei Song, Ronald E. Savage, Caiyun Zhong, Rui-Yang Yang, Darin Kizer, Hui Wu, Erika Volckova, Mark A. Ashwell, Jeffrey G. Supko, Xiaoying He and Thomas C. K. Chan ...
Dave Weigel writes the smartest thing Ive read about the Sarah Palin phenomenon in some time. The problem is that Palin has put the political press in a submissive position, one in which the only information it prints about her...
TY - JOUR. T1 - Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor. AU - Yang, Seung Hyun. AU - Heo, Dan. AU - Lee, Eugene. AU - Kim, Eunjung. AU - Lim, Eun Kyung. AU - Lee, Young Han. AU - Haam, Seungjoo. AU - Suh, Jin Suck. AU - Huh, Yong Min. AU - Yang, Jaemoon. AU - Park, Sahng Wook. PY - 2013/11/29. Y1 - 2013/11/29. N2 - Cancer cells can express specific biomarkers, such as cell membrane proteins and signaling factors. Thus, finding biomarkers and delivering diagnostic agents are important in the diagnosis of cancer. In this study, we investigated a biomarker imaging agent for the diagnosis of hepatic cancers. The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. The utility of the ASGPr-targetable imaging agent, galactosylated MFNP (G-MFNP) was assessed by ...
TY - JOUR. T1 - Asialoglycoprotein receptor phosphorylation and receptor-mediated endocytosis in hepatoma cells. Effect of phorbol esters. AU - Fallon, R. J.. AU - Schwartz, A. L.. PY - 1988/1/1. Y1 - 1988/1/1. N2 - The asialoglycoprotein (ASGP) receptor on Hep G2 cells undergoes constitutive recycling and ligand endocytosis in the presence of phorbol dibutyrate, at a 50% reduced rate relative to control cells (Fallon, R. J., and Schwartz, A. L. (1986) J. Biol. Chem. 261, 15081-15089). The relevance of receptor phosphorylation to these events was investigated by selective immunoprecipitation of surface receptors with polyclonal anti-human ASGP antiserum and pulse-chase labeling with [32P]orthophosphate to identify subcellular locations of initial receptor phosphorylation events as well as the eventual fate of phosphorylated receptor during recycling. The surface immunoprecipitation method recovers greater than 95% of surface ASGP receptors and only 5% or less of intracellular ...
Clone REA608 recognizes the human and rat asialoglycoprotein receptor 1 (ASGPR1) antigen, a single-pass type II membrane protein, also known as C-type lectin domain family 4 member H1 (CLEC4H1) or hepatic lectin H1 (HL-1). ASGPR1 is the major subunit of the asialoglycoprotein receptor (ASGPR) and is specifically expressed on the sinusoidal and basolateral hepatocellular membranes, but not on the bile canalicular membrane. It plays a role in the clearance of desialylated proteins from the serum through endocytosis and lysosomal degradation.Additional information: Clone REA608 displays negligible binding to Fc receptors. - Belgique
0041]It is presently preferred to isolate E1 and/or E2 asialoglycoproteins from crude cell lysates by incubation with an immobilized mannose-binding protein, particularly a lectin such as ConA or GNA. Cells are lysed, e.g., by mechanical disruption in a hypotonic buffer followed by centrifugation to prepare a post-nuclear lysate, and further centrifuged to obtain a crude microsomal membrane fraction. The crude membrane fraction is subsequently solubilized in a buffer containing a detergent, such as Triton X-100, NP40, or the like. This detergent extract is further clarified of insoluble particulates by centrifugation, and the resulting clarified lysate incubated in a chromatography column comprising an immobilized mannose-binding protein, preferably GNA bound to a solid support such as agarose or Sepharose® for a period of time sufficient for binding, typically 16 to 20 hours. The suspension is then applied to the column until E1/E2 begins to appear in the eluent, then incubated in the column ...
Alterations in protein synthesis in primary cultured rat liver parenchymal cells were examined after their exposure to the potent carcinogens, polychlorinated biphenyl (PCB) congeners. Co-planar PCB congeners (3,4,5,3′,4′-PCB and 3,4,5,3′,4′,5′-PCB) (10 nM) induced a protein, the Mr of which was 25,000 (25 k protein) under denaturing conditions. However, non-co-planar PCB congeners and several xenobiotics, which induce microsomal proteins, did not induce the 25 k protein. By using immunoblotting, the 25 k protein was identified as glutathione S-transferase P-form (GST-P, 7-7, EC 2.5.1.18).. ...
Asialoglycoprotein Receptor 1兔多克隆抗体(ab42488)可与大鼠, 人样本反应并经WB, ICC/IF实验严格验证,被2篇文献引用并得到2个独立的用户反馈。
This study highlights that in hepatocytes, SARS-CoV-2 spike protein binding probably involved other receptors and mechanisms than only ACE-2.
Residualizing labels are tracers which remain in lysosomes after uptake and catabolism of the carrier protein and have been especially useful for studies on the sites of plasma protein degradation. Thus far these labels have contained radioactive reporters such as 3H or 125I. In the present paper we describe a fluorescent residualizing label, NN-dilactitol-N′-fluoresceinylethylenediamine (DLF). Modification of asialofetuin (ASF) or rat serum albumin (RSA) with DLF affected neither their normal kinetics of clearance from the rat circulation nor their normal tissue sites of uptake and degradation. After injection of DLF-ASF, fluorescent degradation products were recovered nearly quantitatively in liver and retained with a half-life of about 2 days. Fluorescent degradation products from DLF-RSA were recovered in skin and muscle, and were localized in fibroblasts by fluorescence microscopy. These results confirm previous studies with radioactive residualizing labels in which fibroblasts in ...
A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates the endocytosis of serum glycoproteins.
London, May 19 : In a new study, researchers at the University of California, San Diego School of Medicine have found that the Ashwell receptor, a molecule present in liver of all animals, plays a key role in reducing the coagulation abnormalities during infection and sepsis, significantly improving the probability of survival.. According to the studys principal investigator, Jamey Marth, Ph. D., UCSD Professor of Cellular and Molecular Medicine and Investigator with the Howard Hughes Medical Institute, Sepsis is a life-threatening complication of bacterial infection in the blood is a major cause of death worldwide. The research has solved the longest-standing mystery in glycobiology - a field that studies complex sugar chains called glycans, by stating that the Ashwell receptor plays a major role in helping the body fight off the abnormal and lethal blood clotting caused by bacterial infection.. Till date, the researchers believed that this receptor might help in discarding abnormal proteins ...
Rabbit Polyclonal Anti-Asialoglycoprotein Receptor 2 Antibody. Validated: WB, IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
DNA molecules complexed with an asialoglycoprotein-polycation conjugate, consisting of asialoorosomucoid (ASOR) coupled to poly-l-lysine, can enter hepatocytes which bear receptors for ASOR. We used this receptor-mediated DNA delivery system to deliver plasmid DNA encoding glycoprotein D (gD) of herpes simplex virus type 1 to ASOR-positive cells. Maximum expression of gD protein was seen at 3 days after injection of this preparation in approximately 13% of cells from BALB/c mice [hepatocytes from mice injected intravenously (i.v.) or peritoneal exudate cells from mice injected intraperitoneally (i.p.)]. In comparison with mice injected with either the plasmid vector alone or the gD-containing plasmid uncomplexed to ASOR, mice immunized with gD-containing plasmid complexed with ASOR-poly-l-lysine induced marked antigen-specific CTL responses. BALB/c mice immunized with gD-DNA developed a T-cell-mediated CTL response against target cells expressing gD and MHC class II glycoproteins, but not against cells
A more directed approach to the delivery of therapeutic oligonucleotides specifically to the liver has been to target the asialoglycoprotein receptor (ASGPR) using a suitable glycoconjugate. Indeed, ASGPR is the ideal target for delivery of therapeutic oligonucleotides to the liver since it combines tissue specificity, high expression levels and rapid internalization and turnover. The use of oligonucleotide glycoconjugates has led to significant advances in therapeutic delivery as evidenced by the work of Alnylam Pharmaceuticals which has developed multivalent N-acetylgalactosamine (GalNAc) conjugated siRNAs that bind at nanomolar levels to ASGPR (1). A similar strategy has been applied at Ionis Pharmaceuticals directed at the development of antisense oligonucleotide therapeutics (2 ...
Carbohydrate-oligonucleotide conjugates have become increasingly important in recent years, particularly with regard to an increased uptake and cell availability of siRNA and antisense oligonucleotides. Of particular interest here is N-acetylgalactosamine (GalNAc), which can bind to the asialoglycoprotein receptor (ASGPR) in hepatocytes.. The following picture shows some examples of the attachment of carbohydrates (sugars) to oligonucleotides via different linker structures:. ...
Carbohydrate-oligonucleotide conjugates have become increasingly important in recent years, particularly with regard to an increased uptake and cell availability of siRNA and antisense oligonucleotides. Of particular interest here is N-acetylgalactosamine (GalNAc), which can bind to the asialoglycoprotein receptor (ASGPR) in hepatocytes.. The following picture shows some examples of the attachment of carbohydrates (sugars) to oligonucleotides via different linker structures:. ...
The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid…
1FIH: Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor.
1FIF: Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor.
Acetyl-LDL (Ac-LDL) bound to transformed mouse macrophage J774 cells in a high affinity, saturable and specific manner. When cells were cultured for 24h in the presence of tunicamycin such that incorporation of N-linked sugars into protein but not protein synthesis itself was inhibited significantly, the binding characteristics of Ac-LDL to the cells were unaltered. In this respect the Ac-LDL receptor of J774 cells is similar to the asialoglycoprotein receptor of HepG2 cells. ...
French Catholic philosopher Jacques Maritain once wrote that Americans are the least materialist among the modern peoples which have attained the industrial stage.. Drawing on this sentiment, George Weigel argues that although materialism may reign in America more than it once did, there remains a link between money-making and idealism in these United States that is distinctive, and perhaps even unique.. Pointing to President Calvin Coolidge (no fan of materialism), Weigel emphasizes that Coolidges famous line-the chief business of the American people is business-shouldnt be taken by itself. For Coolidge, and for most Americans (even today), promoting the dignity-conferring effects of business is part of a larger, deeper idealism.. As Weigel explains:. As for wealth, consider Silent Cals remarks at the end of the same speech: We make no concealment of the fact that we want wealth, but there are many other things that we want very much more. We want peace and honor, and that charity ...
Welcome to Biology 1510 for Fall 2017! Select course readings from the menus above by Module: Evolution Ecology Molecules and Metabolism Genetics Integrative Human Health Lecture materials can be downloaded from t-square Instructions for the group video project will be … Continue reading →. ...
K. Schneeberger, Hagmann, J., Ossowski, S., Warthmann, N., Gesing, S., Kohlbacher, O., and Weigel, D., Simultaneous alignment of short reads against multiple genomes, Genome Biol, vol. 10, p. R98, 2009. ...
A number of genes encoding C-type lectin molecules have been mapped to the natural killer gene complex (NKC) at the distal region of mouse chromosome 6 and to a syntenic region on human chromosome 12p12-p13. In addition to those receptors which regulate NK cell function, related structures expressed on other cells types have also been localized to this chromosomal region. Among these are a number of recently characterized genes, including macrophage C-type lectin (MCL), macrophage-inducible C-type lectin (Mincle), dendritic cell immunoreceptor (DCIR) and dendritic cell-associated lectin-2 (Dectin-2). The amino acid sequences comprising the single C-type lectin domains of MCL, Mincle, DCIR and Dectin-2 are shown here to be closely related to each other. These molecules show overall similarity to two groups of animal C-type lectins, groups II and V, which demonstrate type II transmembrane topology. In this study, sequence analysis suggests that MCL, Mincle, DCIR and Dectin-2 represent a subset of group II
Neurobiology of Australian Marsupials von Ashwell Ashwell und Buchbewertungen gibt es auf ReadRate.com. Bücher können hier direkt online erworben werden.
The major and minor forms of the asialoglycoprotein receptor (ASGP-R), designated in the rat as RHL-1 and RHL-2/3, respectively, have traditionally been considered to be expressed exclusively in hepatic parenchymal cells. Northern blot analysis now demonstrates that rat and mouse testis express a receptor similar to the RHL-1 ASGP-R but not the RHL-2/3 receptor. In situ hybridization studies demonstrate that late-stage mouse and rat spermatids are the testicular cells that express the RHL-1 ASGP-R. The rat spermatid RHL-1 receptor has functional binding capability, with a KD of approximately 1.4 x 10(-8) M, and similar characteristics, compared with the hepatic RHL-1 receptor, regarding ligand binding specificity and ion dependence. These findings have major implications for therapeutic procedures attempting to target cytotoxic agents or DNA to hepatocytes using the ASGP-R. In addition, these findings demonstrate that late-stage spermatids are transcriptionally active and suggest that the RHL-1 ...
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The number of Asialoglycoprotein (ASGP) receptors on the hepatocytes of patients with liver disease is reduced and is thus considered a good indicator for the evaluation of liver function. ASGP receptor-targeted radiopharmaceuticals permit a non-invasive way to evaluate total and regional hepatic function and hepatic functional reserve visually and quantitatively. Over the past three decades, a variety of ASGP receptor-targeted probes have been developed with different molecular backbones (albumin, polymer, small-molecular-weight ligand), different glycol-residues (galactose, lactose, N-acetyl-galactosamine) and different chelating systems suitable for radiolabeling with SPECT isotopes (Tc-99m, In-111, Ga-67, (131)/I-125, Sm-153) and PET isotopes (Ga-68, F-18). In this review, we present an overview of ASGP receptor-targeted radiopharmaceuticals, discuss their chemistry, biodistribution, catabolism and challenge as well as application for measurement of liver function ...
William Ashwell Shenstone FIC FRS (1 December 1850 in Wells-next-the-Sea, Norfolk, England - 3 February 1908 in Mullion, Cornwall) was a chemist, schoolmaster and published author. Shenstone was born on 1 December 1850 to James Burt Byron Shenstone, a pharmaceutical chemist in Colchester, and his wife Jemima; he was named after the poet William Shenstone, a distant relative. He was educated at Colchester Royal Grammar School, and later as an apprentice to his father, though, during the latter, he was caught in a fire and suffered from pains throughout his life. He married Jane Mildred, the daughter of the rector of Wootton, on 27 December 1883, with whom he had two children, a son and a daughter. Shenstone died on 3 February 1908 at the Polurrian Hotel, Mullion, Cornwall, at the age of fifty-eight. By trade a schoolmaster, Shenstone joined Clifton College, Bristol, as assistant to William Tilden in 1874, before being appointed science master at Taunton School the next year, and in 1877 at Exeter ...
Exactly. News organizations dont hire conservatives. Therefore, conservatives dont seek careers in journalism and instead become lawyers or accountants or stock brokers or whatever.. Ergo, none of the people making decisions in MSM newsrooms are conservatives. Where set A = employees of news organizations and set B = conservatives, we see that as the closer set AB approaches to zero, the more likely journalists are to view conservatives as The Other, and vice-versa.. If you think such a situation is a good thing, then youre obviously congratulating yourself about Dave Weigels downfall, and you dont want to hear the explanation of my disagreement.. UPDATE VI: Not to say there isnt an upside: Ezra Klein has deep-sixed Journolist as a result of the Weigel affair. Little Miss Attila says the list had become blackmail fodder.. I spoke to Weigel by phone this afternoon. Hes fine, and is currently deciding which of three or four job offers to accept. He admits that he succumbed to hubris, ...
RESULTS: We found a rare noncoding 12-base-pair (bp) deletion (del12) in intron 4 of ASGR1, which encodes a subunit of the asialoglycoprotein receptor, a lectin that plays a role in the homeostasis of circulating glycoproteins. The del12 mutation activates a cryptic splice site, leading to a frameshift mutation and a premature stop codon that renders a truncated protein prone to degradation. Heterozygous carriers of the mutation (1 in 120 persons in our study population) had a lower level of non-HDL cholesterol than noncarriers, a difference of 15.3 mg per deciliter (0.40 mmol per liter) (P=1.0×10(-16)), and a lower risk of coronary artery disease (by 34%; 95% confidence interval, 21 to 45; P=4.0×10(-6)). In a larger set of sequenced samples from Icelanders, we found another loss-of-function ASGR1 variant (p.W158X, carried by 1 in 1850 persons) that was also associated with lower levels of non-HDL cholesterol (P=1.8×10(-3 ...
Presentation of sialic acid (Sia) varies among different tissues and organs within each species, and between species. This diversity has biologically important consequences regarding the recognition of cells by xeno antibodies (Neu5Gc vs Neu5Ac). Sia also plays a central role in inflammation by influencing binding of the asialoglycoprotein receptor 1 (ASGR-1), Siglec-1 (Sialoadhesin), and cellular interactions mediated by the selectin, integrin, and galectin receptor families. This review will focus on what is known about basic Sia structure and function in association with xenotransplantation, how changes in sialylation may occur in this context (through desialylation or changes in sialyltransferases), and how this fundamental pathway modulates adhesive and cell activation pathways that appear to be particularly crucial to homeostasis and inflammation for xenografts ...
Chao, D. (Creator), Borevitz, J. O. (Creator), Atwell, S. (Creator), Huang, Y. S. (Creator), Vilhjalmsson, B. J. (Creator), Willems, G. (Creator), Horton, M. (Creator), Li, Y. (Creator), Meng, D. (Creator), Platt, A. (Creator), Tarone, A. M. (Creator), Hu, T. T. (Creator), Jiang, R. (Creator), Muliyati, N. W. (Creator), Zhang, X. (Creator), Amer, M. A. (Creator), Baxter, I. (Creator), Brachi, B. (Creator), Chory, J. (Creator), Dean, C. (Creator), Debieu, M. (Creator), de Meaux, J. (Creator), Ecker, J. R. (Creator), Faure, N. (Creator), Kniskern, J. M. (Creator), Jones, J. D. G. (Creator), Michael, T. (Creator), Nemri, A. (Creator), Roux, F. (Creator), Salt, D. E. (Creator), Tang, C. (Creator), Todesco, M. (Creator), Traw, M. B. (Creator), Weigel, D. (Creator), Marjoram, P. (Creator), Bergelson, J. (Creator), Nordborg, M. (Creator), Silva, A. (Creator), GEO, 31 Jul 2012. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39679 and 2 more links, ...
Weßling R, Epple P, Altmann S, He Y, Yang L, Henz SR, McDonald N, Wiley K, Bader KC, Gläßer C, Mukhtar MS, Haigis S, Ghamsari L, Stephens AE, Ecker JR, Vidal M, Jones JD, Mayer KF, Ver Loren van Themaat E, Weigel D, Schulze-Lefert P, Dangl JL, Panstruga R, Braun ...
Research project, Figurations of the Martyr in Near Eastern and European Literature, Angelika Neuwirth, Sigrid Weigel, Zentrum für Literatur- und …
TY - JOUR. T1 - Receptor-mediated uptake of human α1-acid glycoprotein into liver parenchymal cells in mice. AU - Matsumoto, Kazuaki. AU - Nishi, Koji. AU - Kikuchi, Mari. AU - Watanabe, Hiroshi. AU - Nakajou, Keisuke. AU - Komori, Hisakazu. AU - Kadowaki, Daisuke. AU - Suenaga, Ayaka. AU - Maruyama, Toru. AU - Otagiri, Masaki. N1 - Funding Information: Acknowledgments: This research was supported Grants-in-aid in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) (18590035 and 20390161).. PY - 2010. Y1 - 2010. N2 - Human α1-acid glycoprotein (AGP), a serum glycoprotein, is thought to have anti-inflammatory effects by a mechanism that is not well understood. In this study, we investigated the pharmacokinetics of AGP in mice using enzymatically modified AGP (AGP with the sialic acids removed, asialo-AGP, and with both sialic acids and galactose removed, agalacto-AGP). It was observed that AGP was eliminated from the circulation slowly, and ...
The incorporation of carbohydrate functional elements in the architecture of polycationic amphiphilic cyclodextrins (paCDs) provides glycosylated paCDs (pGaCDs) that form transfectious nanocomplexes (glycoCDplexes) with pDNA. In this study, we aimed at elucidating the internalization mechanisms at play and their incidence in transfection efficiency for glycoCDplexes formulated with 6-amino-6-deoxy-β-d-glucopyranosyl-appended pGaCDs in comparison with mannosylated and non-glycosylated congeners. Preliminary data showed a relatively high uptake of the 6-aminoglucosylated nanocomplexes by BNL-CL2 hepatocytes that correlated with a strong affinity towards the galactose-specific peanut agglutinin (PNA) lectin, suggesting that the galactose-binding asialoglycoprotein receptor at the surface of hepatocytes might be involved in glycoCDplex internalization. Transfection kinetics, internalization rates and protein expression data in BNL-CL2 ASGPR-expressing cells and COS-7 ASGPR-devoid epithelial cells ...
David Weigel has an article on how things have changed for those who talked about rebranding the Republicans after their 2006 and 2008 loss. Some Republicans were looking at ways the party should change direction and possibly take some less extreme position son some issues. At the moment, with Republicans appearing on the brink of doing well in the off-year elections, it looks like such rebranding might not have been needed. Republicans believe they are doing fine by sticking to their old views or moving even further to the right.. That is a rather short sighted view of the situation. In a two party system one party can have some victories even when on the wrong side of the issues. Many people are bound to vote for the party out of power when the economy is bad. Republicans also have an advantage this year because Democrats are trying to defend a large number of seats which have traditionally been held by Republicans.. Under these conditions it would come as no surprise that the Republicans ...
George Weigel is Americans leading lay authority on the Roman Catholic Church, a biographer of John Paull II and benedict XVI, and one of the most sought-
The long read: For 25 years, invoking this vague and ever-shifting nemesis has been a favourite tactic of the right - and Donald Trumps victory is its greatest triumph
Deloukas P, Earthrowl ME, Grafham DV, Rubenfield M, French L, Steward CA, Sims SK, Jones MC, Searle S, Scott C, Howe K, Hunt SE, Andrews TD, Gilbert JG, Swarbreck D, Ashurst JL, Taylor A, Battles J, Bird CP, Ainscough R, Almeida JP, Ashwell RI, Ambrose KD, Babbage AK, Bagguley CL, Bailey J, Banerjee R, Bates K, Beasley H, Bray-Allen S, Brown AJ, Brown JY, Burford DC, Burrill W, Burton J, Cahill P, Camire D, Carter NP, Chapman JC, Clark SY, Clarke G, Clee CM, Clegg S, Corby N, Coulson A, Dhami P, Dutta I, Dunn M, Faulkner L, Frankish A, Frankland JA, Garner P, Garnett J, Gribble S, Griffiths C, Grocock R, Gustafson E, Hammond S, Harley JL, Hart E, Heath PD, Ho TP, Hopkins B, Horne J, Howden PJ, Huckle E, Hynds C, Johnson C, Johnson D, Kana A, Kay M, Kimberley AM, Kershaw JK, Kokkinaki M, Laird GK, Lawlor S, Lee HM, Leongamornlert DA, Laird G, Lloyd C, Lloyd DM, Loveland J, Lovell J, McLaren S, McLay KE, McMurray A, Mashreghi-Mohammadi M, Matthews L, Milne S, Nickerson T, Nguyen M, Overton-Larty ...
c,Number of Inmates Recorded as having Inflicted Non-fatal Injury on,c, ,c,themselves,c, ,1979,1980,1981,1982,1983 ---------------------------------------------------- Male Establishments Acklington ,1 ,0 ,0 ,1 ,2 Albany ,2 ,1 ,1 ,2 ,0 Aldington ,0 ,3 ,0 ,0 ,1 Appleton Thorn ,1 ,0 ,- ,- ,- Ashford ,23 ,28 ,23 ,19 ,16 Ashwell ,2 ,1 ,1 ,0 ,1 Aylesbury ,4 ,5 ,6 ,3 ,7 Bedford ,4 ,0 ,0 ,0 ,2 Birmingham ,32 ,29 ,30 ,44 ,12 Blantyre House ,3 ,3 ,5 ,1 ,2 Blundeston ,5 ,0 ,3 ,0 ,2 Bristol ,14 ,4 ,7 ,8 ,10 Brixton ,8 ,11 ,13 ,17 ,20 Brockhill ,2 ,1 ,1 ,7 ,3 Buckley Hall ,1 ,4 ,4 ,1 ,2 Camp Hill ,2 ,3 ,2 ,- ,5 Campsfield House ,0 ,1 ,1 ,0 ,0 Canterbury ,19 ,5 ,5 ,2 ,1 Cardiff ,25 ,20 ,8 ,4 ,29 Castington ,0 ,1 ,2 ,5 ,0 Channings Wood ,1 ,0 ,1 ,1 ,2 Chelmsford ,- ,0 ,5 ,8 ,8 Coldingley ,1 ,1 ,1 ,0 ,2 Dartmoor ,2 ,1 ,1 ,3 ,4 Deerbolt ,7 ,6 ,13 ,1 ,0 Dorchester ,3 ,6 ,1 ,4 ,4 Dover ,14 ,8 ,1 ,1 ,10 Durham ,9 ,6 ,8 ,9 ,11 Eastwood Park ,2 ,2 ,1 ,3 ,1 Erlestoke ,0 ,0 ,1 ,3 ,0 Everthorpe ,4 ,19 ,18 ,14 ,4 Exeter ...
TY - JOUR. T1 - The binding of fucose-containing glycoproteins by hepatic lectins. Re-examination of the clearance from blood and the binding to membrane receptors and pure lectins. AU - Lehrman, M. A.. AU - Pizzo, S. V.. AU - Imber, M. J.. AU - Hill, R. L.. PY - 1986. Y1 - 1986. N2 - The nature of the hepatic receptors that bind glycoproteins through fucose at the non-reducing termini of oligosaccharides in glycoproteins has been examined by three different approaches. First, the clearance from blood of intravenously injected glycoproteins was examined in mice with the aid of neoglycoproteins of bovine serum albumin (BSA). The clearance of fucosyl-BSA was rapid and was not strongly inhibited by glycoproteins that inhibit clearance mediated by the galactose or the mannose/N-acetylglucosamine receptors of liver. The clearance of Fucα1,3(Galβ1,4)GlcNAc-BSA (where Fuc is fucose) was inhibited weakly by either Fuc-BSA or Galβ1,4GlcNAc-BSA but strongly by a mixture of the two neoglycoproteins, ...
We already know Weigel is our enemy, by his own words.. ODonnell wont even rule out running as an independent if she loses the primary. She lies about her record and finances, is paying her rent with campaign money, and sold her house to her campaign lawyer to avoid foreclosure.. Castles a RINO, but ODonnell is a lying crook. The latest Rasmussen poll shows Castle beating the Democrat by double digits, and ODonnell losing by double digits.. What is it about lying, crooked losers like ODonnell and Hayworth that attract some conservatives to support them? Have you no ethical standards at all?. ...
Mungall AJ, Palmer SA, Sims SK, Edwards CA, Ashurst JL, Wilming L, Jones MC, Horton R, Hunt SE, Scott CE, Gilbert JG, Clamp ME, Bethel G, Milne S, Ainscough R, Almeida JP, Ambrose KD, Andrews TD, Ashwell RI, Babbage d9e AK, Bagguley CL, Bailey J, Banerjee R, Barker DJ, Barlow KF, Bates K, Beare DM, Beasley H, Beasley O, Bird CP, Blakey S, Bray-Allen S, Brook J, Brown AJ, Brown JY, Burford DC, Burrill W, B 10f8 urton J, Carder C, Carter NP, Chapman JC, Clark SY, Clark G, Clee CM, Clegg S, Cobley V, Collier RE, Collins JE, Colman LK, Corby NR, Coville GJ, Culley KM, Dhami P, Davies J, Dunn M, Earthrowl ME, Ellington AE, Evans KA, Faulkner L, Francis MD, Frankish A, Frankland J, French L, Garner P, Garnett J, Ghori MJ, Gilby LM, Gillson CJ, Glithero RJ, Grafham DV, Grant M, Gribble S, Griffiths C, Griffiths M, Hall R, Halls KS, Hammond S, Harley JL, Hart EA, Heath PD, Heathcott R, Holmes SJ, Howden PJ, Howe KL, Howell GR, Huckle E, Humphray SJ, Humphries MD, Hunt AR, Johnson CM, Joy AA, Kay M, ...
Die globale Coronavirus-Pandemie hat alle Industriezweige auf der ganzen Welt in Mitleidenschaft gezogen, Prostata-spezifischer Antigen (PSA) -Biomarker Markt bildet da keine Ausnahme. Da die Weltwirtschaft nach der Krise von 2009 auf eine schwere Rezession zusteuert, hat Cognitive Market Research eine aktuelle Studie veröffentlicht, die die Auswirkungen dieser Krise auf den Globalen Markt für Schlüsselwörter sorgfältig untersucht und mögliche Maßnahmen zu deren Eindämmung vorschlägt. Bei dieser Pressemitteilung handelt es sich um eine Momentaufnahme der Forschungsstudie. Weitere Informationen können durch Zugriff auf den vollständigen Bericht abgerufen werden. Um Detaillierte Analyse zu erhalten Mailen Sie uns @ [email protected] or call us on +1-312-376-8303.. Erhalten Sie ein kostenloses Exemplar eines Prostata-spezifischer Antigen (PSA) -Biomarker Marktberichts: @ ...
The asialoglycoprotein receptors that bind Sendai virus. and serve as SeV cell entry receptors are highly expressed in liver ... Stockert, R. J. (July 1995). "The asialoglycoprotein receptor: relationships between structure, function, and expression". ... entry by means of the asialoglycoprotein receptor of a Sendai virus mutant lacking its attachment protein". Proceedings of the ... "Sendai virus efficiently infects cells via the asialoglycoprotein receptor and requires the presence of cleaved F0 precursor ...
Weiss, P; Ashwell, G (1989). "The asialoglycoprotein receptor: properties and modulation by ligand". Progress in Clinical and ...
Stockert, R. J. (1995-07-01). "The asialoglycoprotein receptor: relationships between structure, function, and expression". ...
"In vitro binding of the asialoglycoprotein receptor to the beta adaptin of plasma membrane coated vesicles". The EMBO Journal ...
... mannose versus asialoglycoprotein) is a bit misleading as these the asialoglycoprotein receptors are not necessarily galactose ... Another potential characterization of the CLRs can be into mannose receptors and asialoglycoprotein receptors. The mannose ... one of the commonest outer residues of asialo-glycoprotein) specific receptors and even many of this family members can also ... This is another large superfamily of CLRs that includes The classic asialoglycoprotein receptor macrophage galactose-type ...
Ashwell explained that he was not specifically looking for the asialoglycoprotein when he found it. Ashwell died on June 27, ... to discover that a certain receptor in a human's liver is able to recognize a specific glycoprotein called asialoglycoprotein. ...
"Plasticity of the asialoglycoprotein receptor deciphered by ensemble FRET imaging and single-molecule counting PALM imaging". ...
Clearance of IgA is mediated at least in part by asialoglycoprotein receptors, which recognizes galactose-terminating IgA N- ...
... homology with asialoglycoprotein receptors and cartilage proteoglycan core protein". Biochemistry. 26 (21): 6757-64. doi: ...
... asialoglycoprotein receptors) group contains several sub-sub-families, many of which are important to innate immunity. A ...
... where it binds to the asialoglycoprotein receptors on hepatocytes. Galactosamine Globoside (N-Acetylglucosamine) GlcNAc Donald ...
... the hepatic asialoglycoprotein receptors, intestinal sucrose-isomaltase, the erythropoietin receptor, two subunits of the TGFß ... identified the intracellular organelles that mediate recycling of the asialoglycoprotein and transferrin receptors, and ...
Asialoglycoprotein receptor family DC-SIGN (CD209) Langerin (CD207) CLEC10A (CD301, MGL) CLEC5A (MDL1) Dectin 1 subfamily ...
Asialoglycoproteins at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... If terminal sialic acid residues are removed from glycoproteins, the resulting proteins are known as asialoglycoproteins. The ... galactose residues results in rapid clearance of the glycoproteins from the circulation through hepatocyte asialoglycoprotein ...
The asialoglycoprotein receptors are lectins which bind asialoglycoprotein and glycoproteins from which a sialic acid has been ... The asialoglycoprotein receptor has been demonstrated to have high expression on the surface of hepatocytes [1], several human ... Asialoglycoprotein+receptor at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... Lactobionic acid has been used as a targeting moiety for drug delivery to cells expressing asialoglycoprotein receptors. ...
... is a protein that in humans is encoded by the ASGR1 gene. This gene encodes a subunit of the ... The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by ... "Entrez Gene: Asialoglycoprotein receptor 1". Retrieved 2018-10-02. Yik JH, Saxena A, Weigel PH (June 2002). "The minor subunit ... The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a ...
Asialoglycoprotein. *Avidin. *B-cell activating factor. *4-1BB ligand. *Cholesterylester transfer protein ...
Asialoglycoprotein receptor. *Tumor necrosis factor receptor. *Immunoglobulin superfamily. *N-Acetylglucosamine receptor. * ...
Asialoglycoprotein receptor. *Tumor necrosis factor receptor. *Immunoglobulin superfamily. *N-Acetylglucosamine receptor. * ...
Asialoglycoprotein receptor. *Aspartate carbamoyltransferase. *Aspartate-semialdehyde dehydrogenase. *Asprosin. *Ataxin 7. * ...
Asialoglycoprotein receptor. *Tumor necrosis factor receptor. *Immunoglobulin superfamily. *N-Acetylglucosamine receptor. * ...
Asialoglycoproteins at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... If terminal sialic acid residues are removed from glycoproteins, the resulting proteins are known as asialoglycoproteins. The ... galactose residues results in rapid clearance of the glycoproteins from the circulation through hepatocyte asialoglycoprotein ...
The asialoglycoprotein receptors are lectins which bind asialoglycoprotein and glycoproteins from which a sialic acid has been ... The asialoglycoprotein receptor has been demonstrated to have high expression on the surface of hepatocytes [1], several human ... Asialoglycoprotein+receptor at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... Lactobionic acid has been used as a targeting moiety for drug delivery to cells expressing asialoglycoprotein receptors. ...
What is asialoglycoprotein? Meaning of asialoglycoprotein medical term. What does asialoglycoprotein mean? ... Looking for online definition of asialoglycoprotein in the Medical Dictionary? asialoglycoprotein explanation free. ... This study evaluated the usefulness of asialoglycoprotein receptor scintigraphy with [sup.. Asialoglycoprotein receptor ... asialoglycoprotein. Also found in: Wikipedia. a·si·a·lo·gly·co·pro·tein. (āsī-alō-glīkō-prōtēn), A glycoprotein without a ...
Asialoglycoprotein receptor 2Add BLAST. 311. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical ... asialoglycoprotein receptor activity Source: UniProtKB ,p>Traceable Author Statement,/p> ,p>Used for information from review ... "Asialoglycoprotein receptor facilitates infection of PLC/PRF/5 cells by HEV through interaction with ORF2.". Zhang L., Tian Y. ... "Sequence of a second human asialoglycoprotein receptor: conservation of two receptor genes during evolution.". Spiess M., ...
Rabbit polyclonal Asialoglycoprotein Receptor 1/HL-1 antibody. Validated in WB, IHC and tested in Human. Cited in 1 publication ... Anti-Asialoglycoprotein Receptor 1/HL-1 antibody. See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies. ... Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab49355) at 0.6 µg/ml + Fetal liver lysate at 10 µg with 5% skim milk in PBS. ... Protein - Recombinant Human Asialoglycoprotein Receptor 1/HL-1 (ab151893) SDS-PAGE, HPLC ...
What is asialoglycoprotein receptor? Meaning of asialoglycoprotein receptor medical term. What does asialoglycoprotein receptor ... Looking for online definition of asialoglycoprotein receptor in the Medical Dictionary? asialoglycoprotein receptor explanation ... This study evaluated the usefulness of asialoglycoprotein receptor scintigraphy with [sup.. Asialoglycoprotein receptor ... asialoglycoprotein receptor. Also found in: Wikipedia. a·si·a·lo·gly·co·pro·tein re·cep·tor. a surface receptor found in ...
A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates ... Asialoglycoprotein Receptor. Subscribe to New Research on Asialoglycoprotein Receptor A C-type lectin that is a cell surface ... Hepatic Asialoglycoprotein; Receptors, Asialoglycoprotein; Receptors, Asialoorosomucoid; Asialoglycoprotein Receptors ... Asialoglycoprotein; Receptor, Asialoorosomucoid; Asialoglycoprotein Receptor, Hepatic; Asialoorosomucoid Binding Protein; ...
Rabbit Polyclonal Anti-Asialoglycoprotein Receptor 2 Antibody. Validated: WB, IHC, IHC-P. Tested Reactivity: Human. 100% ... Additional Asialoglycoprotein Receptor 2 Products. Asialoglycoprotein Receptor 2 NBP1-52958 * Asialoglycoprotein Receptor 2 ... Home » Asialoglycoprotein Receptor 2 » Asialoglycoprotein Receptor 2 Antibodies » Asialoglycoprotein Receptor 2 Antibody ... Blogs on Asialoglycoprotein Receptor 2. There are no specific blogs for Asialoglycoprotein Receptor 2, but you can read our ...
Ausgesuchte Qualitäts-Hersteller für Asialoglycoprotein Receptor 2 Antikörper. Hier bestellen. ... Monoklonale und polyklonale Asialoglycoprotein Receptor 2 Antikörper für viele Methoden. ... Asialoglycoprotein Receptor 2 (ASGR2) Antigen-Profil Protein Überblick This gene encodes a subunit of the asialoglycoprotein ... anti-Asialoglycoprotein Receptor 2 Antikörper 31 Asialoglycoprotein Receptor 2 (ASGR2) Antikörper von 11 Herstellern verfügbar ...
Effect of chronic ethanol administration on total asialoglycoprotein receptor content and intracellular processing of ... of a representative asialoglycoprotein, asialoorosomucoid (ASOR), by the liver (Casey et al. (1987) J. Biol. Chem. 262, 2704- ...
Kaninchen Polyklonal Asialoglycoprotein Receptor 2 Antikörper für WB. Order anti-Asialoglycoprotein Receptor 2 Antikörper ... Target Details Asialoglycoprotein Receptor 2 Bilder Handhabung Anwendungsinformationen zurück nach oben Target Details ... Produktdetails anti-Asialoglycoprotein Receptor 2 Antikörper Bilder Handhabung Anwendungsinformationen Antigendetails zurück ... The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by ...
Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor. ... Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor ... Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor ... Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor ...
Effects of vanadate on receptor-mediated endocytosis of asialoglycoproteins in isolated rat hepatocytes TROND BERG TROND BERG ... TROND BERG, OLA GUDMUNDSEN, GRETE M. KINDBERG; Effects of vanadate on receptor-mediated endocytosis of asialoglycoproteins in ...
ABSTRACTThe asialoglycoprotein (ASGP) receptor is an abundant hepatocyte-specific receptor involved in receptor-mediated ... The asialoglycoprotein (ASGP) receptor is an abundant hepatocyte-specific receptor involved in receptor-mediated endocytosis. ...
The asialoglycoprotein (ASGP) receptor is an abundant hepatocyte-specific receptor involved in receptor-mediated endocytosis. ... The asialoglycoprotein (ASGP) receptor is an abundant hepatocyte-specific receptor involved in receptor-mediated endocytosis. ...
Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ... Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ... Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ... Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ...
The major subunit of the human asialoglycoprotein receptor contains signals for efficient endocytosis and specific basolateral ... The major subunit of the human asialoglycoprotein receptor contains signals for efficient endocytosis and specific basolateral ... Related signals for endocytosis and basolateral sorting of the asialoglycoprotein receptor. Journal of Biological Chemistry, ...
Fuhrer, C; Geffen, I; Huggel, K; Spiess, M (1994). The two subunits of the asialoglycoprotein receptor contain different ... The human asialoglycoprotein receptor, an endocytic transport receptor of the basolateral surface of hepatocytes, is a hetero- ... The human asialoglycoprotein receptor, an endocytic transport receptor of the basolateral surface of hepatocytes, is a hetero- ... Thus, whereas the endocytosis signal of the hetero-oligomeric asialoglycoprotein receptor resides exclusively in subunit H1, ...
Late-stage spermatids are characterized by expression of the "liver-specific" asialoglycoprotein receptor, RHL-1.. B E Huber ... Late-stage spermatids are characterized by expression of the "liver-specific" asialoglycoprotein receptor, RHL-1.. B E Huber ... Late-stage spermatids are characterized by expression of the "liver-specific" asialoglycoprotein receptor, RHL-1.. B E Huber ... The major and minor forms of the asialoglycoprotein receptor (ASGP-R), designated in the rat as RHL-1 and RHL-2/3, respectively ...
When preparations of rat liver receptor for asialoglycoproteins (rat hepatic lectin, (RHL] are examined by dodecyl sulfate- ... Asialoglycoprotein receptor promotes cancer metastasis by activating the EGFR-ERK pathway.. *Suguru Ueno, Marija K Mojić, ... Differential ligand binding by two subunits of the rat liver asialoglycoprotein receptor.. *Nelson Ruiz, Kurt Drickamer ... Thyroglobulin binding and TSH regulation of the RHL-1 subunit of the asialoglycoprotein receptor in rat thyroid.. *Francesco ...
Asialoglycoprotein receptor 2 (Asgr2) Recombinant Protein-NP_001300854.1 (MBS7053730) product datasheet at MyBioSource, ... The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by ... This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical ... "Impaired receptor-mediated endocytosis by the asialoglycoprotein receptor in ethanol-fed mice: implications for studying the ...
... imaging with a radiolabeled asialoglycoprotein analog, Technetium-99m-diethylenetriaminepentaacetic acid-galactosyl-human serum ... Evaluation of GalNAc-siRNA Conjugate Activity in Pre-clinical Animal Models with Reduced Asialoglycoprotein Receptor Expression ... Hepatic lobar differences in progression of chronic liver disease: correlation of asialoglycoprotein scintigraphy and hepatic ... correlation of asialoglycoprotein scintigraphy and hepatic functional reserve.}, author={Shigenobu Matsuzaki and Masahiko Onda ...
Recycling of the asialoglycoprotein receptor and the effect of lysosomotropic amines in hepatoma cells. ... Effect of lysosomotropic amines on the secretory pathway and on the recycling of the asialoglycoprotein receptor in human ... Rat hepatocytes bind to synthetic galactoside surfaces via a patch of asialoglycoprotein receptors. ... Ligand- and weak base-induced redistribution of asialoglycoprotein receptors in hepatoma cells. ...
"Asialoglycoproteins" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... This graph shows the total number of publications written about "Asialoglycoproteins" by people in this website by year, and ... They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by ... Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. ...
An "isolated" HCV asialoglycoprotein intends an HCV asialoglycoprotein composition which is at least 35% pure. [0023]Mannose- ... Patent application title: Hepatitis C virus asialoglycoproteins. Inventors: Michael Houghton Robert O. Ralston Frank Marcus ... Patent application title: Hepatitis C virus asialoglycoproteins. Inventors: Robert O. Ralston (Danville, CA, US) Frank Marcus ( ... In the case of E1 and E2 (as expressed in our systems), the asialoglycoprotein appears to be the final form. E1 (envelope ...
The asialoglycoprotein-receptor (ASGP-R) is a C-type lectin predominantly expressed on the sinusoidal surface of mammalian ... Targeting hepatocytes via the asialoglycoprotein-receptor. 2010, PhD Thesis, University of Basel, Faculty of Science. ...
Asialoglycoprotein Receptor 1兔多克隆抗体(ab42488)可与大鼠, 人样本反应并经WB, ICC/IF实验 ... Anti-Asialoglycoprotein Receptor 1抗体. 参阅全部 Asialoglycoprotein Receptor 1 一抗. ... Anti-Asialoglycoprotein Receptor 1 antibody (ab42488) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at ... Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1 antibody (ab42488)This image is
title = "Tyrosine phosphorylation of the asialoglycoprotein receptor",. abstract = "The asialoglycoprotein (ASGP) receptor ... Tyrosine phosphorylation of the asialoglycoprotein receptor. / Fallon, R. J.. In: Journal of Biological Chemistry, Vol. 265, No ... Fallon, R. J. (1990). Tyrosine phosphorylation of the asialoglycoprotein receptor. Journal of Biological Chemistry, 265(6), ... Fallon, R. J. / Tyrosine phosphorylation of the asialoglycoprotein receptor. In: Journal of Biological Chemistry. 1990 ; Vol. ...
Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor Mcvicker, Benita L. Omaha VA Medical Center, ... Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor. Mcvicker, Benita L. / Omaha VA Medical Center ... Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor. Mcvicker, Benita L. / Omaha VA Medical Center ... Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor. Mcvicker, Benita L. / Omaha VA Medical Center ...
Fingerprint Dive into the research topics of Tyrosine phosphorylation of the asialoglycoprotein receptor. Together they form ...
  • The asialoglycoprotein receptor has been demonstrated to have high expression on the surface of hepatocytes [1], several human carcinoma cell lines[2] and liver cancers. (wikipedia.org)
  • and,aA alternatively, in the same ApoE knockout model, silencing activity was found to be restored by the use of an exogenous GalNAc ligand that targets the iLNP to the asialoglycoprotein receptor (ASGR) expressed on hepatocytes, with as little as 0. (thefreedictionary.com)
  • On the sinusoidal surface of the hepatocytes, asialoglycoprotein receptors are expressed abundantly which removes the asialo (galactose-terminal) glycoproteins from the sinusoidal circulation by internalizing the bound protein via endocytosis. (thefreedictionary.com)
  • The human asialoglycoprotein receptor, an endocytic transport receptor of the basolateral surface of hepatocytes, is a hetero-oligomer of two homologous subunits H1 and H2. (uzh.ch)
  • The asialoglycoprotein-receptor (ASGP-R) is a C-type lectin predominantly expressed on the sinusoidal surface of mammalian hepatocytes and is responsible for the blood-clearance of desialylated glycoproteins by receptormediated endocytosis. (unibas.ch)
  • The asialoglycoprotein (ASGP) receptor undergoes constitutive endocytosis through the coated pit/coated vesicle pathway in hepatocytes. (elsevier.com)
  • In this study, we investigated the background of hepatocyte proliferation for the springboard of control between proliferation and differentiation of hepatocytes, and we focused attention to the asialoglycoprotein receptors (ASGP-R) of the hepatocytes. (elsevier.com)
  • Recent studies suggest that the H1 subunit of the carbohydrate recognition domain (H1CRD) of the asialoglycoprotein receptor is used as an entry site into hepatocytes by hepatitis A and B viruses and Marburg virus. (unibas.ch)
  • Uptake of desialylated adiponectin by isolated primary rat hepatocytes was also accelerated, suggesting a role for the hepatic asialoglycoprotein receptor. (nih.gov)
  • The number of Asialoglycoprotein (ASGP) receptors on the hepatocytes of patients with liver disease is reduced and is thus considered a good indicator for the evaluation of liver function. (ihep.ac.cn)
  • Asialoglycoprotein Receptor Function of Hepatocytes in Patients with Autoimmune Hepatitis. (nii.ac.jp)
  • Function of Asialoglycoprotein Receptor of Hepatocytes in Patients with Wilson's Disease. (nii.ac.jp)
  • Here, we report the development and validation of a unique magnetic cell separation system, which allows enumeration, immunomorphologic identification, and genetic analysis of CTCs from peripheral blood samples of HCC patients, mediated by the interaction of the asialoglycoprotein receptor exclusively expressed on hepatocytes with its ligand. (aacrjournals.org)
  • Sendai virosomes, derived from Sendai viral envelopes, home to hepatocytes based on the liver specific expression of asialoglycoprotein receptors (ASGPRs) which are recognized by the Sendai virosomal fusion (F) proteins. (biomedcentral.com)
  • Sendai virosomes specifically fuse with the hepatocytes through their fusion (F) protein's terminal galactose moiety, that binds specifically with asialoglycoprotein receptors (ASGPRs) present only on the surface of hepatocytes [ 14 ]. (biomedcentral.com)
  • Further, iLNPs were engineered with the carbohydrate N-acetylgalactosamine (GalNAc) to achieve targeting to the asialoglycoprotein receptor (ASGPR) as an alternative targeting strategy for the hepatic delivery of RNAi therapeutics. (thefreedictionary.com)
  • The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. (elsevier.com)
  • The asialoglycoprotein receptor (ASGPR) is considered one of the target antigens in the pathogenesis of autoimmune chronic active hepatitis (AIH). (elsevier.com)
  • The purpose of this research was to recognize the induction and pathogenic potential of autoantibodies against a liver-specific asialoglycoprotein receptor (ASGPR) in the course of hepadnavirus infection. (mun.ca)
  • These findings indicate that virus-induced autoantibodies against hepatic ASGPR have the potential to directly contribute to liver injury and to the distortion of the hepatocyte clearance of asialoglycoproteins in viral hepatitis. (mun.ca)
  • Likewise, asialoglycoprotein receptor (ASGPR) was up-regulated in UCMSCs upon differentiation. (sigmaaldrich.com)
  • Lactoferrin (Lf) is a potential-targeting ligand for hepatocellular carcinoma (HCC) cells because of its specific binding with asialoglycoprotein receptor (ASGPR). (dovepress.com)
  • Asialoglycoprotein receptor (ASGPR) as target autoantigen in liver autoimmunity: Lost and found. (biomedsearch.com)
  • The exposure of the subterminal galactose residues results in rapid clearance of the glycoproteins from the circulation through hepatocyte asialoglycoprotein receptors on Kuppfer cells. (wikipedia.org)
  • The asialoglycoprotein receptors are lectins which bind asialoglycoprotein and glycoproteins from which a sialic acid has been removed to expose galactose residues. (wikipedia.org)
  • Lactobionic acid has been used as a targeting moiety for drug delivery to cells expressing asialoglycoprotein receptors. (wikipedia.org)
  • such proteins are recognized by asialoglycoprotein receptors and are targeted for degradation. (thefreedictionary.com)
  • Autoantibodies detected in CLDs target a wide repertoire of host antigens, which include proteins from the nucleus, the cytoskeleton, liver kidney microsomes, the mitochondria, and the cytoplasm of neutrophilic granulocytes, as well as liver soluble antigens, asialoglycoprotein receptors, TAA, and stress proteins [21]. (thefreedictionary.com)
  • Both Ebola and Marburg viruses use multiple entry mechanisms via receptors such as: hepatocyte receptor asialoglycoprotein , folate receptor [alpha], dendritic-cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), DC-SIGN-related factors, [beta]1 integrin receptors and human macrophage galactose- and N-acetylgalactosamine-specific C type lectin. (thefreedictionary.com)
  • The galactose group is recognizable to the hepatic asialoglycoprotein receptors. (thefreedictionary.com)
  • We show that an electroactive interface confined with densely clustered galactosyl ligands is able to ingeniously recognize the asialoglycoprotein receptors on live Hep-G2 cells employing simple electrochemical techniques. (nature.com)
  • N-lactosyl-dioleoylphosphatidylethanolamine (Lac-DOPE) was synthesized and evaluated as a liver-specific targeting ligand via asialoglycoprotein receptors for liposomal delivery of doxorubicin. (dovepress.com)
  • In the presence of 20 mM lactose, the uptake of Lac-L-calcein was inhibited, suggesting that asialoglycoprotein receptors mediated the observed cellular uptake. (dovepress.com)
  • The cDNA cloning was based on the structural homology to hepatic asialoglycoprotein receptors. (jimmunol.org)
  • While the asialoglycoprotein receptor would be considered the founder member of this group of receptors, the in vivo evidence for its function is less compelling than the results for the mannose receptor, which has well defined roles in clearance of sulfated glycoprotein hormones and mannose-bearing glycoproteins released at sites of inflammation. (functionalglycomics.org)
  • Synthetic peptides corresponding to ASGR2 (asialoglycoprotein receptor 2) The peptide sequence was selected from the N terminal of ASGR2. (novusbio.com)
  • 31 Asialoglycoprotein Receptor 2 (ASGR2) Antikörper von 11 Herstellern verfügbar auf www.antikoerper-online.de. (antikoerper-online.de)
  • These Asgr2 knock-out mice exhibit reduced expression of asialoglycoprotein receptor 1 (ASGR1). (jax.org)
  • Subsequent cell experiments revealed that due to the deletion, the serried gene -- ASGR1 -- is unable to establish the normal structure and function of the protein called the asialoglycoprotein receptor. (thefreedictionary.com)
  • We investigated the expression of ACE-2, binding of biotinylated spike protein, biotinylated spike 1 and inhibition of binding by unlabeled spike protein, two neutralizing antibodies and an antibody directed against the hepatocyte asialoglycoprotein receptor 1 (ASGr1). (cherrybiotech.com)
  • A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. (curehunter.com)
  • When preparations of rat liver receptor for asialoglycoproteins (rat hepatic lectin, (RHL] are examined by dodecyl sulfate-polyacrylamide gel electrophoresis, multiple polypeptide species are found to be present. (semanticscholar.org)
  • The purpose of this study was to define the distribution of the asialoglycoprotein receptor (ASGP-R) main peptide, rat hepatic lectin (RHL)-1, within the rat liver lobule and to investigate its possible modulation in physiological states characterised by marked changes of receptorial expression. (univaq.it)
  • Western Blot: Asialoglycoprotein Receptor 2 Antibody [NBP1-52958] - Fetal Lung tissue at a concentration of 0.5ug/ml. (novusbio.com)
  • Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody [NBP1-52958] - Human Intestine Tissue, antibody concentration 4-8ug/ml. (novusbio.com)
  • Immunohistochemistry: Asialoglycoprotein Receptor 2 Antibody [NBP1-52958] - Human cilia Cellular data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X. (novusbio.com)
  • Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor antibody. (elsevier.com)
  • HCC cells were bound by biotinylated asialofetuin, a ligand of asialoglycoprotein receptor, and subsequently magnetically labeled by antibiotin antibody-coated magnetic beads, followed by magnetic separation. (aacrjournals.org)
  • If terminal sialic acid residues are removed from glycoproteins, the resulting proteins are known as asialoglycoproteins. (wikipedia.org)
  • Examination of the pharmacokinetics of FST-ΔHBS-Fc molecules containing variable sialic acid content in asialoglycoprotein receptor 1 (ASPGR-1) knockout mice supports the receptor's role as part of the clearance mechanism of the molecules. (aspetjournals.org)
  • The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). (acris-antibodies.com)
  • Differential ligand binding by two subunits of the rat liver asialoglycoprotein receptor. (semanticscholar.org)
  • Conference abstract describing galactosamine as a ligand of the asialoglycoprotein receptor. (strath.ac.uk)
  • 99m Tc-DTPA-galactosyl human serum albumin ( 99m Tc-GSA) is a newly developed liver imaging ligand which specifically binds to asialoglycoprotein receptor on the hepatic cell surface. (fujita-hu.ac.jp)
  • Our results showed that ethanol metabolism impaired internalization of asialoorosomucoid, a ligand for the asialoglycoprotein receptor. (hindawi.com)
  • Our patient had tested negative for acute and chronic viral hepatitis as well as asialoglycoprotein receptor antibodies. (thefreedictionary.com)
  • Background: This gene encodes a subunit of the asialoglycoprotein receptor. (antikoerper-online.de)
  • The major subunit of the human asialoglycoprotein receptor contains signals for efficient endocytosis and specific basolateral expression in polarized Madin-Darby canine kidney cells, both of which are located within its 40-residue cytoplasmic domain. (uzh.ch)
  • Thus, whereas the endocytosis signal of the hetero-oligomeric asialoglycoprotein receptor resides exclusively in subunit H1, polarized transport to the basolateral domain of Madin-Darby canine kidney cells may involve two signals, only one of which is active for endocytosis. (uzh.ch)
  • Thyroglobulin binding and TSH regulation of the RHL-1 subunit of the asialoglycoprotein receptor in rat thyroid. (semanticscholar.org)
  • This gene encodes a subunit of the asialoglycoprotein receptor. (mybiosource.com)
  • Asialoglycoprotein receptor deficiency in mice lacking the minor receptor subunit. (jax.org)
  • Chronic ethanol administration markedly impairs the process of receptor-mediated endocytosis (RME) of a representative asialoglycoprotein, asialoorosomucoid (ASOR), by the liver (Casey et al. (nih.gov)
  • The asialoglycoprotein (ASGP) receptor is an abundant hepatocyte-specific receptor involved in receptor-mediated endocytosis. (ovid.com)
  • In particular, our laboratory identified multiple ethanol-induced alterations in the process of receptor- mediated endocytosis (RME) and more specifically, we discovered that ethanol treatment resulted in marked impairments in the function of the hepatocyte-specific asialoglycoprotein (ASGP) receptor. (grantome.com)
  • In the present study, transfected cells were maintained in the absence or presence of 25 mM ethanol for 7 days, and alterations in endocytosis by the asialoglycoprotein receptor were determined. (hindawi.com)
  • Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. (jefferson.edu)
  • In 1974, Gilbert Ashwell and Anatol Morell discovered a receptor in the liver that recognizes particular glycoproteins, dubbed asialoglycoproteins. (nature.com)
  • The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. (antikoerper-online.de)
  • Late-stage spermatids are characterized by expression of the "liver-specific" asialoglycoprotein receptor, RHL-1. (aspetjournals.org)
  • Effect of chronic ethanol administration on total asialoglycoprotein receptor content and intracellular processing of asialoorosomucoid in isolated. (nih.gov)
  • Effect of lysosomotropic amines on the secretory pathway and on the recycling of the asialoglycoprotein receptor in human hepatoma cells. (rupress.org)
  • The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. (antikoerper-online.de)
  • As such, our results implied that GAL-AN and GAL-DOX-AN have specific interaction with HepG2 cells via the recognition of GAL and asialoglycoprotein receptor, which renders GAL-AN a promising anticancer drug delivery carrier for liver cancer therapy. (curehunter.com)
  • Quan GL, Pan X, Wang ZH, Wu QL, Li G, Dian LH et al (2015) Lactosaminated mesoporous silica nanoparticles for asialoglycoprotein receptor targeted anticancer drug delivery. (biomedcentral.com)
  • The major and minor forms of the asialoglycoprotein receptor (ASGP-R), designated in the rat as RHL-1 and RHL-2/3, respectively, have traditionally been considered to be expressed exclusively in hepatic parenchymal cells. (aspetjournals.org)
  • ab42488 staining Asialoglycoprotein Receptor 1 (red) in rat hepatocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). (abcam.cn)
  • Macrophages express several types of C-type lectins, such as macrophage mannnose receptor and macrophage asialoglycoprotein binding protein (M-ASGP-BP). (jimmunol.org)
  • Using the hepatocyte-specific asialoglycoprotein receptor and the substance that specifically binds to it (i. (thefreedictionary.com)
  • Evaluation of GalNAc-siRNA Conjugate Activity in Pre-clinical Animal Models with Reduced Asialoglycoprotein Receptor Expression. (semanticscholar.org)
  • Modulation of mannose and asialoglycoprotein receptor expression determines glycoprotein hormone half-life at critical points in the reproductive cycle. (semanticscholar.org)
  • The two subunits of the asialoglycoprotein receptor contain different sorting information. (uzh.ch)
  • Synthetic peptide within Human Asialoglycoprotein Receptor 1/HL-1 aa 100-149 (internal sequence). (abcam.com)
  • A 3D model for the human hepatic asialoglycoprotein receptor (ASGP-R). (thefreedictionary.com)
  • We studied the hepatic functional reserve in the lobes of the liver in 28 patients with chronic liver disease and 13 controls using single photon emission computed tomography (SPECT) imaging with a radiolabeled asialoglycoprotein analog, Technetium-99m-diethylenetriaminepentaacetic acid-galactosyl-human serum albumin (Tc-99m GSA). (semanticscholar.org)
  • Hepatic lobar differences in progression of chronic liver disease: correlation of asialoglycoprotein scintigraphy and hepatic functional reserve. (semanticscholar.org)