Endogenous glycoproteins from which SIALIC ACID has been removed by the action of sialidases. They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by adsorptive ENDOCYTOSIS they are delivered to LYSOSOMES for degradation. Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. They are elevated in serum of patients with HEPATIC CIRRHOSIS or HEPATITIS.
A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates the endocytosis of serum glycoproteins.
A family of calcium-binding alpha-globulins that are synthesized in the LIVER and play an essential role in maintaining the solubility of CALCIUM in the BLOOD. In addition the fetuins contain aminoterminal cystatin domains and are classified as type 3 cystatins.
An enzyme that oxidizes galactose in the presence of molecular oxygen to D-galacto-hexodialdose. It is a copper protein. EC 1.1.3.9.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Cell surface molecules on cells of the immune system that specifically bind surface molecules or messenger molecules and trigger changes in the behavior of cells. Although these receptors were first identified in the immune system, many have important functions elsewhere.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
The rate dynamics in chemical or physical systems.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

Stabilization of poly-L-lysine/DNA polyplexes for in vivo gene delivery to the liver. (1/431)

We are developing a self-assembling non-viral in vivo gene delivery vehicle based on poly-l-lysine and plasmid DNA. We have characterized poly-l-lysines of different chain lengths for DNA condensation and strength of DNA binding. Poly-l-lysine chains >20 residues bound DNA efficiently in physiological saline, while shorter chains did not. Attachment of asialoorosomucoid to PLL increased the PLL chain length required for efficient DNA binding in saline and for efficient DNA condensation. By electron microscopy, poly-l-lysine/DNA polyplexes appeared as toroids 25-50 nm in diameter or rods 40-80 nm long; conjugation of asialoorosomucoid to the polylysine component increased the size of resulting polyplexes to 50-90 nm. In water, poly-l-lysine and asialoorosomucoid-PLL polyplexes have effective diameters of 46 and 87.6 nm, respectively. Polyplexes containing only poly-l-lysine and DNA aggregated in physiological saline at all charge ratios and aggregated at neutral charge ratios in water. Attachment of asialoorosomucoid lessened, but did not eliminate, the aggregation of PLL polyplexes, and did not result in efficient delivery of polyplexes to hepatocytes. Conjugation of polyethylene glycol to poly-l-lysine sterically stabilized resulting polyplexes at neutral charge ratios by shielding the surfaces. For efficient in vivo gene delivery, polyplexes will need to be sterically stabilized to prevent aggregation and interaction with serum components.  (+info)

Nucleotide exchange in genomic DNA of rat hepatocytes using RNA/DNA oligonucleotides. Targeted delivery of liposomes and polyethyleneimine to the asialoglycoprotein receptor. (2/431)

Chimeric RNA/DNA oligonucleotides have been shown to promote single nucleotide exchange in genomic DNA. A chimeric molecule was designed to introduce an A to C nucleotide conversion at the Ser365 position of the rat factor IX gene. The oligonucleotides were encapsulated in positive, neutral, and negatively charged liposomes containing galactocerebroside or complexed with lactosylated polyethyleneimine. The formulations were evaluated for stability and efficiency in targeting hepatocytes via the asialoglycoprotein receptor. Physical characterization and electron microscopy revealed that the oligonucleotides were efficiently encapsulated within the liposomes, with the positive and negative formulations remaining stable for at least 1 month. Transfection efficiencies in isolated rat hepatocytes approached 100% with each of the formulations. However, the negative liposomes and 25-kDa lactosylated polyethyleneimine provided the most intense nuclear fluorescence with the fluorescein-labeled oligonucleotides. The lactosylated polyethyleneimine and the three different liposomal formulations resulted in A to C conversion efficiencies of 19-24%. In addition, lactosylated polyethyleneimine was also highly effective in transfecting plasmid DNA into isolated hepatocytes. The results suggest that both the liposomal and polyethyleneimine formulations are simple to prepare and stable and give reliable, reproducible results. They provide efficient delivery systems to hepatocytes for the introduction or repair of genetic mutations by the chimeric RNA/DNA oligonucleotides.  (+info)

Copper and zinc ions differentially block asialoglycoprotein receptor-mediated endocytosis in isolated rat hepatocytes. (3/431)

Asialoglycoprotein receptors on hepatocytes lose endocytic and ligand binding activity when hepatocytes are exposed to iron ions. Here, we report the effects of zinc and copper ions on the endocytic and ligand binding activity of asialoglycoprotein receptors on isolated rat hepatocytes. Treatment of cells at 37 degrees C for 2 h with ZnCl2 (0-220 microM) or CuCl2 (0-225 microM) reversibly blocked sustained endocytosis of 125I-asialoorosomucoid by up to 93% (t1/2 = 62 min) and 99% (t1/2 = 54 min), respectively. Cells remained viable during such treatments. Zinc- and copper-treated cells lost approximately 50% of their surface asialoglycoprotein receptor ligand binding activity; zinc-treated cells accumulated inactive asialoglycoprotein receptors intracellularly, whereas copper-treated cells accumulated inactive receptors on their surfaces. Cells treated at 4 degrees C with metal did not lose surface asialoglycoprotein receptor activity. Exposure of cells to copper ions, but not to zinc ions, blocked internalization of prebound 125I-asialoorosomucoid, but degradation of internalized ligand and pinocytosis of the fluid-phase marker Lucifer Yellow were not blocked by metal treatment. Zinc ions reduced diferric transferrin binding and endocytosis on hepatocytes by approximately 33%; copper ions had no inhibitory effects. These findings are the first demonstration of a specific inhibition of receptor-mediated endocytosis by non-iron transition metals.  (+info)

Asialoglycoprotein receptor scintigraphy in evaluation of auxiliary partial orthotopic liver transplantation. (4/431)

The purpose of this study was to evaluate asialoglycoprotein receptor scintigraphy in the post-transplant monitoring of liver graft and native liver functions in recipients of auxiliary partial orthotopic liver transplantation (APOLT) from living donors. METHODS: We performed 36 asialoglycoprotein receptor scintigraphies on 13 patients who had undergone APOLT for noncirrhotic metabolic liver diseases or for small-for-size grafts. The portal vein of the native liver was separated in 12 patients. Anterior dynamic images including the heart and both livers were obtained for 16 min after intravenous injection of 99mTc-diethylenetriamine pentaacetic acid-galactosyl human serum albumin (GSA), and thereafter static SPECT images of both livers were obtained. Uptake rates from the blood to the graft and to the native liver were determined separately by Patlak plot graphical analysis. Relative uptake of GSA by the graft was calculated from transverse SPECT images. The relative volume of the graft liver was determined by CT. RESULTS: The relative uptake of GSA by the graft was higher or increased more rapidly than the relative volume of the graft in 8 of 11 patients with no severe complications concerning the graft. The relative uptake by severely damaged graft liver in 2 patients was much lower than the relative volume. The uptake rate of GSA by the graft was low in these 2 patients. The uptake rate by the native liver decreased when the portal vein was separated. CONCLUSION: The relative uptake of GSA was a better indicator of graft liver function than was anatomic volume. The uptake rate provided additional independent information of each liver. Asialoglycoprotein receptor scintigraphy is useful for distinguishing and monitoring the graft and native liver functions in patients who had undergone APOLT.  (+info)

Molecular cloning and expression of Galbeta1,3GalNAc alpha2, 3-sialyltransferase from human fetal liver. (5/431)

Based on the sequences of the highly conserved segments in the previously cloned sialyltransferases, a cDNA encoding Galbeta1, 3GalNAc alpha2,3-sialyltransferase (SIATFL) has been isolated from human fetal liver. Expression analysis of the gene has been performed with various carcinoma cell lines, fetal tissues, fetal and adult liver and both hepatoma and the surrounding tissue from the same liver. The SIATFL gene was expressed poorly in fetal liver and in adult liver, slightly in hepatoma and highly in the surrounding tissue of hepatoma. The cDNA encoding the putative active domain was expressed in COS-1, Escherichia coli, and Pichia pastoris. The recombinant protein expressed in COS-1 could catalyse the transfer of NeuAc from CMP-NeuAc to asialo-fetuin. No enzyme activity was detected with a 32-kDa protein in E. coli and both 32-kDa and 41-kDa proteins in P. pastoris. These results suggested that correct glycosylation of the enzyme might play a key role in its folding that may be directly related to the enzymatic activity.  (+info)

Characterization of recombinant and plant-derived mistletoe lectin and their B-chains. (6/431)

Mistletoe lectin I (pML) and its isoforms ML II and III constitute the active principle in extract preparations from mistletoe, commonly used as immunomodulator in adjuvant tumour therapy. The heterodimeric disulfide-linked cytotoxic protein is classified as type II ribosome inactivating protein (RIP). Recently, the sequence coding for the mistletoe lectin prepro-protein was identified and the existence of a single intron-free gene was shown [Eck, J., Langer, M., Mockel, B., Baur, A., Rothe, M., Zinke, H. & Lentzen, H. (1999) Eur. J. Biochem. 264, 775-784]. The aim of this study was to prepare pure and homogeneous rMLB-chain as well as rML heterodimer for studying the carbohydrate binding specificity of recombinant versus natural protein and its contribution to the observed cytotoxic effect. Expression in E. coli resulted in the production of insoluble protein (inclusion bodies). A procedure for generating correctly folded, biochemically and biologically active rMLB was established starting from the insoluble single chain. Carbohydrate binding and specificity of pMLB and rMLB were analysed by a competitive enzyme linked lectin assay (ELLA). Asialofetuin was able to compete with binding of both chains (50% at 0.8 microM). The specificity of the B-chains to lactose was more distinct with halfmaximal competition at 4.9 mM (pMLB) and > 90 mM (rMLB), respectively. Furthermore, in a coassociation process rMLA- and rMLB inclusion bodies were associated in one step by defined dilution yielding active rML-heterodimer. The activities of recombinant (rML) and plant derived mistletoe lectin (pML) were compared. Cytotoxicity was determined using MOLT-4 cells and enzymatic rRNA N-glycosidase activity was measured in a coupled transcription/translation assay. The IC50 values of the two heterodimers were similar in both assays; rMLB-chain did not show any cytotoxic effect. In the ELLA with lactose as a competitor 50% competition of binding to asialofetuin was achieved at 1.6 mM (rML) and 1.8 mM (pML). Hence, using three different assays we found no significant differences between the recombinant protein and the glycosylated form of ML. Comparing the biological activities of the single chains with those of the heterodimer we conclude, that both, lectin activity and the rRNA N-glycosidase activity, are prerequisites for the cytotoxic effects on target cells.  (+info)

Evaluation of the components of the chylomicron remnant removal mechanism by use of the isolated perfused mouse liver. (7/431)

The isolated perfused mouse liver was utilized to evaluate the relative contribution of various molecules believed to participate in the removal of chylomicron remnants by the liver. Sixty percent of asialofetuin was removed from the perfusate per pass; bovine serum albumin was not removed. Normal mouse livers removed chylomicron remnants more efficiently (40-50%/pass) than nascent chylomicrons (10-20%/pass). The fractional removal rate of remnants decreased as their concentration in the perfusate increased demonstrating saturability. Remnant removal by livers of low density lipoprotein receptor-deficient (LDLRD) mice paralleled that of normal mice at low remnant concentrations (0.05, 0.2 microg protein/ml); as concentration increased (4-16 microg protein/ml), removal by LDLRD livers was reduced. About 50% of the capacity to remove remnants was due to the LDL receptor. The role of the LDLR-related protein (LRP) was estimated using the receptor-associated protein (RAP). Four microg/ml of RAP inhibited only LRP; it reduced the removal of remnants by 30-40% in normal livers. When RAP was included in the perfusate of LDLRD livers, remnant removal persisted but was diminished, particularly late in the perfusion; the capacity was approximately 30% of controls. The present study has established that there is more than one mechanism operating for the removal of chylomicron remnants by the liver, provides estimates of the concentration of each to the removal of remnants, and indicates a method for further studies. It is concluded that in normal livers, the LDL receptor has the greatest capacity for removing chylomicron remnants. The LRP contributes to the process as well and a third component, perhaps "sequestration," accounts for up to 30% of the capacity for the initial removal of chylomicron remnants.  (+info)

Fluid phase endocytosis and galactosyl receptor-mediated endocytosis employ different early endosomes. (8/431)

Endocytosis may originate both in coated pits and in uncoated regions of the plasma membrane. In hepatocytes it has been shown that fluid phase endocytosis (here defined as 'pinocytosis') is unaffected by treatments that arrest coated pit-mediated endocytosis, indicating that pinocytosis is primarily a clathrin-independent process. In this study we have tried to determine possible connections between pinocytosis and clathrin-dependent endocytosis in rat hepatocytes by means of subcellular fractionation, electron microscopy, and by assessing the influence of inhibitors of clathrin-dependent endocytosis on pinocytosis. As marker for clathrin-dependent endocytosis was used asialoorosomucoid (AOM) labelled with [(125)I]tyramine cellobiose ([(125)I]TC). [(125)I]TC-labelled bovine serum albumin ([(125)I]TC-BSA) was found to be a useful marker for pinocytosis. Its uptake in the cells is not saturable, and any remnants of [(125)I]TC-BSA associated with the cell surface could be removed by incubating the cells with 0.3% pronase at 0 degrees C for 60 min. The data obtained by electron microscopy and by subcellular fractionation suggested that early after initiation of uptake (<15 min) [(125)I]TC-BSA and [(125)I]TC-AOM were present in different endocytic vesicles. The two probes probably join prior to their entrance in the lysosomal compartment. The relation between endocytosis via coated pits and pinocytosis was also studied with techniques that induced a selective density shift either in the clathrin-dependent pathway (by AOM-HRP) or in the pinocytic pathway (by allowing uptake of AuBSA). Both treatments indicated that the two probes ([(125)I]TC-AOM and [(125)I]TC-BSA) were early after uptake, at least partly, in separate endocytic compartments. The different distribution of the fluid phase marker and the ligand (internalised via coated pits) was not due to a difference in the rate at which they enter a later compartment, since a lowering of the incubation temperature to 18 degrees C, which should keep the probes in the early endosomes, did not affect their early density distribution. Incubation of cells in a hypertonic medium reduced uptake both of [(125)I]TC-AOM and [(125)I]TC-BSA; the uptake of [(125)I]TC-AOM was, however, reduced much more than that of the fluid phase marker. This finding supports the notion that the two probes enter the cells via different routes.  (+info)

Asialoglycoproteins at the US National Library of Medicine Medical Subject Headings (MeSH) Gross, Hans Jürgen; Brossmer, ... Regoeczi, E.; Chindemi, P. A. (1981-01-01). "The net weight of the rabbit liver and its relevance for asialoglycoprotein ... If terminal sialic acid residues are removed from glycoproteins, the resulting proteins are known as asialoglycoproteins. The ... Reinhard (1987-06-01). "N-Acetyl-4-deoxy-d-neuraminic acid is activated and transferred on to asialoglycoprotein". ...
The asialoglycoprotein receptor contains two subunits, asialoglycoprotein receptor 1 (ASGR1) and asialoglycoprotein receptor 2 ... The asialoglycoprotein receptors (ASGPR) are lectins which bind asialoglycoprotein and glycoproteins from which a sialic acid ... of the asialoglycoprotein form hydrogen bonds with the CRD of the asialoglycoprotein receptor; proper positioning and ... The human asialoglycoprotein receptors composed of two units, H1 and H2. Each of these units have their N-terminus within the ...
... is a protein that in humans is encoded by the ASGR1 gene. This gene encodes a subunit of the ... The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by ... "Entrez Gene: Asialoglycoprotein receptor 1". Retrieved 2018-10-02. Yik JH, Saxena A, Weigel PH (June 2002). "The minor subunit ... The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a ...
The asialoglycoprotein receptors that bind Sendai virus. and serve as SeV cell entry receptors are highly expressed in liver ... Stockert RJ (July 1995). "The asialoglycoprotein receptor: relationships between structure, function, and expression". ... entry by means of the asialoglycoprotein receptor of a Sendai virus mutant lacking its attachment protein". Proceedings of the ... "Sendai virus efficiently infects cells via the asialoglycoprotein receptor and requires the presence of cleaved F0 precursor ...
Weiss, P; Ashwell, G (1989). "The asialoglycoprotein receptor: properties and modulation by ligand". Progress in Clinical and ...
Stockert, R. J. (1995-07-01). "The asialoglycoprotein receptor: relationships between structure, function, and expression". ...
... mannose versus asialoglycoprotein) is a bit misleading as these the asialoglycoprotein receptors are not necessarily galactose ... Another potential characterization of the CLRs can be into mannose receptors and asialoglycoprotein receptors. The mannose ... one of the commonest outer residues of asialo-glycoprotein) specific receptors and even many of this family members can also ... This is another large superfamily of CLRs that includes the classic asialoglycoprotein receptor macrophage galactose-type ...
Ashwell explained that he was not specifically looking for the asialoglycoprotein when he found it. Ashwell died on June 27, ... to discover that a certain receptor in a human's liver is able to recognize a specific glycoprotein called asialoglycoprotein. ...
"Plasticity of the asialoglycoprotein receptor deciphered by ensemble FRET imaging and single-molecule counting PALM imaging". ...
Beltzer JP, Spiess M (December 1991). "In vitro binding of the asialoglycoprotein receptor to the beta adaptin of plasma ...
Clearance of IgA is mediated at least in part by asialoglycoprotein receptors, which recognizes galactose-terminating IgA N- ...
... homology with asialoglycoprotein receptors and cartilage proteoglycan core protein". Biochemistry. 26 (21): 6757-64. doi: ...
... asialoglycoprotein receptors) group contains several sub-sub-families, many of which are important to innate immunity. A ...
... where it binds to the asialoglycoprotein receptors on hepatocytes. Galactosamine Globoside (N-Acetylglucosamine) GlcNAc Donald ...
... the hepatic asialoglycoprotein receptors, intestinal sucrose-isomaltase, the erythropoietin receptor, two subunits of the TGFß ... identified the intracellular organelles that mediate recycling of the asialoglycoprotein and transferrin receptors, and ...
... and adheres to the asialoglycoprotein receptor on urethral epithelium. LOS is highly stimulatory to the human immune system. ...
Asialoglycoprotein receptor family DC-SIGN (CD209) Langerin (CD207) CLEC10A (CD301, MGL) CLEC5A (MDL1) Dectin 1 subfamily ...
Potentiation of proliferation of some but not all human colon carcinoma cell lines by immobilized hepatic asialoglycoprotein ... Potentiation of proliferation of some but not all human colon carcinoma cell lines by immobilized hepatic asialoglycoprotein ...
In summary, the asialoglycoprotein receptor is important. It is a functional receptor. When there is impaired function in the ... Casey focused on the hepatic asialoglycoprotein receptor (ASGPR), present in high densities on hepatocytes, which binds ...
5. Asialoglycoprotein receptor-mediated gene transfer using novel galactosylated cationic liposomes.. Kawakami S; Yamashita F; ... The minor subunit splice variants, H2b and H2c, of the human asialoglycoprotein receptor are present with the major subunit H1 ... 6. Overexpression of human methylmalonyl CoA mutase in mice after in vivo gene transfer with asialoglycoprotein/polylysine/DNA ... Targeted delivery of DNA using YEE(GalNAcAH)3, a synthetic glycopeptide ligand for the asialoglycoprotein receptor.. Merwin JR ...
Asialoglycoprotein receptor 1 is a novel PCSK9-independent ligand of liver LDLR cleaved by furin. Susan-Resiga D, Girard E, ...
McAbee DD, Jiang X. Copper and zinc ions differentially block asialoglycoprotein receptor-mediated endocytosis in isolated rat ... inhibits the internalization of asialoglycoprotein and transferrin receptor (TfR) in hepatocytes (39), we next examined whether ...
Asialoglycoproteins Preferred Term Term UI T003612. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1985). ... Asialoglycoproteins Preferred Concept UI. M0001810. Registry Number. 0. Scope Note. Endogenous glycoproteins from which SIALIC ... Asialoglycoprotein Term UI T001085930. Date08/03/2020. LexicalTag NON. ThesaurusID NLM (2022). ... Asialoglycoprotein Receptor. Public MeSH Note. 85. History Note. 85. Date Established. 1985/01/01. Date of Entry. 1984/05/30. ...
GalNac binds to the liver asialoglycoprotein receptor, favoring the internalization of these GalNac-conjugated siRNAs into the ...
... designed to enable targeted delivery of RNAi therapeutics to hepatocytes by way of uptake by the asialoglycoprotein receptor. ...
At the time, that was extremely timely because Gilbert Ashwell at the NIH had just discovered the asialoglycoprotein receptor, ...
Asialoglycoproteins - Preferred Concept UI. M0001810. Scope note. Endogenous glycoproteins from which SIALIC ACID has been ... They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by ... They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by ... Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. ...
Asialoglycoproteins Preferred Term Term UI T003612. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1985). ... Asialoglycoproteins Preferred Concept UI. M0001810. Registry Number. 0. Scope Note. Endogenous glycoproteins from which SIALIC ... Asialoglycoprotein Term UI T001085930. Date08/03/2020. LexicalTag NON. ThesaurusID NLM (2022). ... Asialoglycoprotein Receptor. Public MeSH Note. 85. History Note. 85. Date Established. 1985/01/01. Date of Entry. 1984/05/30. ...
Pachikov, A. N., Gough, R. R., Christy, C. E., Morris, M. E., Casey, C. A., LaGrange, C. A., Bhat, G., Kubyshkin, A. V., Fomochkina, I. I., Zyablitskaya, E. Y., Makalish, T. P., Golubinskaya, E. P., Davydenko, K. A., Eremenko, S. N., Riethoven, J. J. M., Maroli, A. S., Payne, T. S., Powers, R., Lushnikov, A. Y., Macke, A. J., & 1 othersPetrosyan, A., Dec 2021, In: Journal of Experimental and Clinical Cancer Research. 40, 1, 289.. Research output: Contribution to journal › Article › peer-review ...
... asialoglyco-protein,noun,E0010723,sialoglyco-protein,noun,E0206822,no a,asialoglycoprotein,noun,E0010723,sialoglycoprotein,noun ... asialo-glycoprotein,noun,E0010723,sialo-glycoprotein,noun,E0206822,no a,asialo-oligosaccharide,noun,E0303512,sialo- ...
Effect of gal/GalNAc regioisomerism in galactosylated liposomes on asialoglycoprotein receptor-mediated hepatocyte-selective ...
Asialoglycoproteins. Endogenous glycoproteins from which SIALIC ACID has been removed by the action of sialidases. They bind ... Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. ... VirusFluorescent DyesTrypsinAsialoglycoproteinsCalnexinalpha-MannosidaseGene Products, envIon ChannelsSodiumIndolizines ... tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by adsorptive ...
Jones, B. R., Bhalla, R. B., Mladek, J., Kaleya, R. N., Gralla, R. J., Alcock, N. W., Schwartz, M. K., Young, C. W. & Reidenberg, M. M., Apr 1980, In: Clinical Pharmacology and Therapeutics. 27, 4, p. 557-562 6 p.. Research output: Contribution to journal › Article › peer-review ...
Portal-Systemic shunts reduce asialoglycoprotein receptor density in rats. J Nucl Med. 2001 Jan; 42(1):110-6. Colquhoun SD, ...
The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by ... The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a ... This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical ... Asialoglycoprotein Receptor 2 Protein / Cell Adhesion Molecule. / Asialoglycoprotein Receptor 2. DTO Classes. Protein. / ...
The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by ... The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a ... This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical ... Asialoglycoprotein Receptor 2 Protein / Cell Adhesion Molecule. / Asialoglycoprotein Receptor 2. DTO Classes. Protein. / ...
The serum half-life is regulated by the expression of liver asialo-glycoprotein receptors. These receptors bind nonsialylated ... The serum half-life is regulated by the expression of liver asialo-glycoprotein receptors. These receptors bind nonsialylated ... The serum half-life is regulated by the expression of liver asialo-glycoprotein receptors. ... glycoproteins on free galactose residues and bound asialo-glycoproteins are removed from the serum.by endocytosis. As a ...
Ascorbate Peroxidases N0000006439 Ascorbic Acid N0000169160 Asialoglycoprotein Receptor N0000169172 Asialoglycoproteins ...
Asialoglycoprotein Receptor. *Autoreceptors. *Cysteine Loop Ligand-Gated Ion Channel Receptors. *Folate Receptors, GPI-Anchored ...
He is world-renowned for co-discovering the asialoglycoprotein receptor in the liver, also referred to as the Ashwell-Morell ...
Recycling of the asialoglycoprotein receptor and the effect of lysosomotropic amines in hepatoma cells.. 98:732-738. 1984 ...
Triantennary N-acetyl galactosamine is a high affinity ligand for liver cell surface specific asialoglycoprotein receptor ( ...
... endocytosis of asialoglycoproteins by isolated rat hepatocytes. AB - The function of intracellular asialoglycoprotein receptors ... Binding to the asialoglycoprotein receptor was abolished by the addition of excess asialoorosomucoid and, under these ... These results indicate that rat asialotransferrin is processed by the asialoglycoprotein uptake pathway in the same way as ... AB - We have investigated the effect of temperature on the content of surface asialoglycoprotein receptors on isolated rat ...
His Small interfering RNA study integrates concerns from other disciplines, such as Asialoglycoprotein receptor, ...
The Asialoglycoprotein Receptor Minor Subunit Gene Contributes to Pharmacokinetics of Factor VIII Concentrates in Hemophilia A. ...
In all cases, recovery to control levels of receptor-mediated endocytosis by the asialoglycoprotein receptor was partially ... 24 The latter studies focused on the asialoglycoprotein receptor, a hepatocyte-specific receptor, which exhibits decreased ...
  • 5. Asialoglycoprotein receptor-mediated gene transfer using novel galactosylated cationic liposomes. (nih.gov)
  • 14. Gene transfer into hepatocytes using asialoglycoprotein receptor mediated endocytosis of DNA complexed with an artificial tetra-antennary galactose ligand. (nih.gov)
  • 15. Fate of DNA targeted to the liver by asialoglycoprotein receptor-mediated endocytosis in vivo. (nih.gov)
  • Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. (nih.gov)
  • Effect of gal/GalNAc regioisomerism in galactosylated liposomes on asialoglycoprotein receptor-mediated hepatocyte-selective targeting in vivo . (bvsalud.org)
  • 9. alpha3-galactosylated glycoproteins can bind to the hepatic asialoglycoprotein receptor. (nih.gov)
  • The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. (nih.gov)
  • 18. Targeted delivery of DNA using YEE(GalNAcAH)3, a synthetic glycopeptide ligand for the asialoglycoprotein receptor. (nih.gov)
  • Asialoglycoprotein receptor 1 is a novel PCSK9-independent ligand of liver LDLR cleaved by furin. (nih.gov)
  • Triantennary N-acetyl galactosamine is a high affinity ligand for liver cell surface specific asialoglycoprotein receptor (ASGPR). (nadcro.com)
  • 13. The minor subunit splice variants, H2b and H2c, of the human asialoglycoprotein receptor are present with the major subunit H1 in different hetero-oligomeric receptor complexes. (nih.gov)
  • This gene encodes a subunit of the asialoglycoprotein receptor. (nih.gov)
  • GalNAc-siRNA conjugates are Alnylam's proprietary delivery platform, designed to enable targeted delivery of RNAi therapeutics to hepatocytes by way of uptake by the asialoglycoprotein receptor. (drugdiscoverynews.com)
  • He is world-renowned for co-discovering the asialoglycoprotein receptor in the liver, also referred to as the Ashwell-Morell receptor. (nih.gov)
  • 6. Overexpression of human methylmalonyl CoA mutase in mice after in vivo gene transfer with asialoglycoprotein/polylysine/DNA complexes. (nih.gov)
  • His Small interfering RNA study integrates concerns from other disciplines, such as Asialoglycoprotein receptor, Oligonucleotide, Gene knockdown and Cell biology. (research.com)
  • They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. (nih.gov)
  • 9 For good examples galactose-functionalized NPs can be selectively accumulated in hepatocellular carcinoma cell collection HepG2 that expresses asialoglycoprotein receptor (ASGP-R).10 Also 2 NPs can target mammary tumor cells11 and migrate across the blood brain barrier12 where glucose transporters (GLUT) are highly indicated. (technuc.com)

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