A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates the endocytosis of serum glycoproteins.
Endogenous glycoproteins from which SIALIC ACID has been removed by the action of sialidases. They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by adsorptive ENDOCYTOSIS they are delivered to LYSOSOMES for degradation. Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. They are elevated in serum of patients with HEPATIC CIRRHOSIS or HEPATITIS.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A family of calcium-binding alpha-globulins that are synthesized in the LIVER and play an essential role in maintaining the solubility of CALCIUM in the BLOOD. In addition the fetuins contain aminoterminal cystatin domains and are classified as type 3 cystatins.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Cell surface molecules on cells of the immune system that specifically bind surface molecules or messenger molecules and trigger changes in the behavior of cells. Although these receptors were first identified in the immune system, many have important functions elsewhere.
Male germ cells derived from the haploid secondary SPERMATOCYTES. Without further division, spermatids undergo structural changes and give rise to SPERMATOZOA.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.
The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA.
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
Systems in which an intracellular signal is generated in response to an intercellular primary messenger such as a hormone or neurotransmitter. They are intermediate signals in cellular processes such as metabolism, secretion, contraction, phototransduction, and cell growth. Examples of second messenger systems are the adenyl cyclase-cyclic AMP system, the phosphatidylinositol diphosphate-inositol triphosphate system, and the cyclic GMP system.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.
Inorganic compounds that contain zinc as an integral part of the molecule.
Inorganic or organic compounds containing trivalent iron.
Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.

A novel, orally administered nucleoside analogue, OGT 719, inhibits the liver invasive growth of a human colorectal tumor, C170HM2. (1/376)

OGT 719 is a novel p.o. bioavailable nucleoside analogue in which galactose is incorporated onto the fluoropyrimidine moiety of the cytotoxic agent 5-fluorouracil (5-FU). OGT 719 has been designed to reduce the systemic toxicity normally associated with 5-FU while retaining activity against disease localized in the liver, in which it may be preferentially localized through the asialoglycoprotein receptor (ASGP-R). We report studies confirming the activity of OGT 719 in inhibiting growth of metastatic human colorectal tumors in the liver of nude mice. The human colorectal cancer cell line C170HM2 readily forms liver metastases in vivo. Oral administration of 1500 mg/kg/day OGT 719 inhibited liver tumor burden by 95% compared with vehicle control, without any observable signs of toxicity. When the tumor burden was increased and the same OGT 719 treatment was compared with a standard clinical dose regimen of 25 mg/kg/day 5-FU/leucovorin given i.v., both treatments were equally efficacious, although 5-FU/leucovorin treatment started 7 days earlier. In contrast to 5-FU, OGT 719 is p.o. bioavailable and has a plasma half-life between 1.5 and 3 h. Several colorectal cancer cell lines express the asialoglycoprotein receptor, although no significant levels can be detected in C170HM2 cells, consistent with the observation that OGT 719 is approximately 3 log orders of magnitude less potent in vitro than 5-FU. Flux through thymidylate synthase, as measured by 3H release from [3H]dUrd, was inhibited by OGT 719 at 4 h. The notable difference in the potency of OGT 719 efficacy on C170HM2 cells in vitro and in vivo supports our model of liver-specific activation of OGT 719. As our data suggest, OGT 719 may significantly inhibit growth of metastatic colorectal tumors in the liver in vivo. This hypothesis is presently being explored in clinical trials for primary hepatocellular carcinoma and colorectal liver metastases.  (+info)

Nucleotide exchange in genomic DNA of rat hepatocytes using RNA/DNA oligonucleotides. Targeted delivery of liposomes and polyethyleneimine to the asialoglycoprotein receptor. (2/376)

Chimeric RNA/DNA oligonucleotides have been shown to promote single nucleotide exchange in genomic DNA. A chimeric molecule was designed to introduce an A to C nucleotide conversion at the Ser365 position of the rat factor IX gene. The oligonucleotides were encapsulated in positive, neutral, and negatively charged liposomes containing galactocerebroside or complexed with lactosylated polyethyleneimine. The formulations were evaluated for stability and efficiency in targeting hepatocytes via the asialoglycoprotein receptor. Physical characterization and electron microscopy revealed that the oligonucleotides were efficiently encapsulated within the liposomes, with the positive and negative formulations remaining stable for at least 1 month. Transfection efficiencies in isolated rat hepatocytes approached 100% with each of the formulations. However, the negative liposomes and 25-kDa lactosylated polyethyleneimine provided the most intense nuclear fluorescence with the fluorescein-labeled oligonucleotides. The lactosylated polyethyleneimine and the three different liposomal formulations resulted in A to C conversion efficiencies of 19-24%. In addition, lactosylated polyethyleneimine was also highly effective in transfecting plasmid DNA into isolated hepatocytes. The results suggest that both the liposomal and polyethyleneimine formulations are simple to prepare and stable and give reliable, reproducible results. They provide efficient delivery systems to hepatocytes for the introduction or repair of genetic mutations by the chimeric RNA/DNA oligonucleotides.  (+info)

Copper and zinc ions differentially block asialoglycoprotein receptor-mediated endocytosis in isolated rat hepatocytes. (3/376)

Asialoglycoprotein receptors on hepatocytes lose endocytic and ligand binding activity when hepatocytes are exposed to iron ions. Here, we report the effects of zinc and copper ions on the endocytic and ligand binding activity of asialoglycoprotein receptors on isolated rat hepatocytes. Treatment of cells at 37 degrees C for 2 h with ZnCl2 (0-220 microM) or CuCl2 (0-225 microM) reversibly blocked sustained endocytosis of 125I-asialoorosomucoid by up to 93% (t1/2 = 62 min) and 99% (t1/2 = 54 min), respectively. Cells remained viable during such treatments. Zinc- and copper-treated cells lost approximately 50% of their surface asialoglycoprotein receptor ligand binding activity; zinc-treated cells accumulated inactive asialoglycoprotein receptors intracellularly, whereas copper-treated cells accumulated inactive receptors on their surfaces. Cells treated at 4 degrees C with metal did not lose surface asialoglycoprotein receptor activity. Exposure of cells to copper ions, but not to zinc ions, blocked internalization of prebound 125I-asialoorosomucoid, but degradation of internalized ligand and pinocytosis of the fluid-phase marker Lucifer Yellow were not blocked by metal treatment. Zinc ions reduced diferric transferrin binding and endocytosis on hepatocytes by approximately 33%; copper ions had no inhibitory effects. These findings are the first demonstration of a specific inhibition of receptor-mediated endocytosis by non-iron transition metals.  (+info)

Targeted delivery of oligodeoxynucleotides to parenchymal liver cells in vivo. (4/376)

Anti-sense oligodeoxynucleotides (ODNs) hold great promise for correcting the biosynthesis of clinically relevant proteins. The potential of ODNs for modulating liver-specific genes might be increased by preventing untimely elimination and by improving the local bioavailability of ODNs in the target tissue. In the present study we have assessed whether the local ODN concentration can be enhanced by the targeted delivery of ODNs through conjugation to a ligand for the parenchymal liver cell-specific asialoglycoprotein receptor. A capped ODN (miscellaneous 20-mer sequence) was derivatized with a ligand with high affinity for this receptor, N2-[N2-(N2,N6-bis{N-[p-(beta-d-galactopyranosyloxy) anilino] thiocarbamyl}-L-lysyl)-N6-(N-{p-[beta-D -galactopyranosyloxy] anilino} thiocarbamyl)-L-lysyl]-N6-[N- (p-{beta-D-galactopyranosyloxy}anilino)thiocarbamyl]-L-lysine (L3G4) (Kd 6.5+/-0.2 nM, mean+/-S.D.). Both the uptake studies in vitro and the confocal laser scan microscopy studies demonstrated that L3G4-ODN was far more efficiently bound to and taken up by parenchymal liver cells than underivatized ODN. Studies in vivo in rats showed that hepatic uptake could be greatly enhanced from 19+/-1% to 77+/-6% of the injected dose after glycoconjugation. Importantly, specific ODN accumulation of ODN into parenchymal liver cells was improved almost 60-fold after derivatization with L3G4, and could be attributed to the asialoglycoprotein receptor. In conclusion, the scavenger receptor-mediated elimination pathway for miscellaneous ODN sequences can be circumvented by direct conjugation to a synthetic tag for the asialoglycoprotein receptor. In this manner a crucial requisite is met towards the application of ODNs in vivo to modulate the biosynthesis of parenchymal liver cell-specific genes such as those for apolipoprotein (a), cholesterol ester transfer protein and viral proteins.  (+info)

Expression of asialoglycoprotein receptor in human fetal liver. (5/376)

Asialoglycoprotein (ASGP) receptor, which exists in hepatic parenchymal cells, plays important roles in the clearance of desialylated glycoproteins from the circulation. To study the expression of ASGP receptor in human fetal liver, specimens were obtained after therapeutic/spontaneous abortion or at autopsy with informed consent. Reverse transcription polymerase chain reaction and immunohistochemical staining showed that ASGP receptor was present as early as 15 weeks of gestation in human fetal liver. In liver at 15 weeks-gestation it was stained uniformly in cytoplasm as seen in HepG2 cells. As gestation proceed the ASGP receptor was frequently observed on the cell surface and intracellular staining showed a spotted distribution. We conclude that the expression of ASGP receptor is an early event in the developmental process of fetal liver. Furthermore, the distribution of ASGP receptor changes as the liver develops.  (+info)

Enhancement of tumor-to-nontumor localization ratios by hepatocyte-directed blood clearance of antibodies labeled with certain residualizing radiolabels. (6/376)

To increase tumor-to-nontumor localization ratios of injected radiolabeled antibodies (Abs), several interrelated methods were used. METHODS: The model systems used were two human carcinoma xenografts grown in nude mice, targeted by antibodies RS11 (antiepithelial glycoprotein-2) or MN-14 (anticarcinoembryonic antigen). The Abs were conjugated with biotin and 111In-benzyl diethylenetriamine pentaacetic acid, and, at various times after injection, were cleared by intraperitoneal injection of galactosylated streptavidin, which delivers the complexes to hepatocytes. The radiolabel used was selected because it is retained within tumors after catabolism of the Ab by the tumor cell but is quite rapidly excreted from hepatocytes into bile. RESULTS: With blood clearance induced at 24 h, and dissection 5 h later, high tumor-to-nontumor ratios were attained. Depending on the model used, tumor-to-blood ratios were 16:1 to 31:1, and tumor-to-nontumor ratios for the kidney, lungs and bone were also high and greatly increased by the clearance regimen. Despite clearance into the liver, tumor-to-liver ratios remained >1, due to fairly rapid biliary excretion of the label. The absolute antibody uptake by the tumors was also high, because 24 h was allowed for the Ab to penetrate and bind to cells within the subcutaneous tumors. CONCLUSION: The method described produced high tumor-to-nontumor ratios at 1 d after injection and may be advantageous for tumor imaging with antibodies. Radiation dosimetry calculations indicate that there is only a slight advantage with this approach for radioimmunotherapy.  (+info)

Follicular thyroglobulin (TG) suppression of thyroid-restricted genes involves the apical membrane asialoglycoprotein receptor and TG phosphorylation. (7/376)

Follicular thyroglobulin (TG) decreases expression of the thyroid-restricted transcription factors, thyroid transcription factor (TTF)-1, TTF-2, and Pax-8, thereby suppressing expression of the sodium iodide symporter, thyroid peroxidase, TG, and thyrotropin receptor genes (Suzuki, K., Lavaroni, S., Mori, A., Ohta, M., Saito, J., Pietrarelli, M., Singer, D. S., Kimura, S., Katoh, R., Kawaoi, A. , and Kohn, L. D. (1997) Proc. Natl. Acad. Sci. U. S. A. 95, 8251-8256). The ability of highly purified 27, 19, or 12 S follicular TG to suppress thyroid-restricted gene expression correlates with their ability to bind to FRTL-5 thyrocytes and is inhibited by a specific antibody to the thyroid apical membrane asialoglycoprotein receptor (ASGPR), which is related to the ASGPR of liver cells. Phosphorylating serine/threonine residues of TG, by autophosphorylation or protein kinase A, eliminates TG suppression and enhances transcript levels of the thyroid-restricted genes 2-fold in the absence of a change in TG binding to the ASGPR. Follicular TG suppression of thyroid-restricted genes is thus mediated by the ASPGR on the thyrocyte apical membrane and regulated by a signal system wherein phosphorylation of serine/threonine residues on the bound ligand is an important component. These data provide a hitherto unsuspected role for the ASGPR in transcriptional signaling, aside from its role in endocytosis. They establish a functional role for phosphorylated serine/threonine residues on the TG molecule.  (+info)

Segmental genomic replacement in embryonic stem cells by double lox targeting. (8/376)

We have applied Cre-mediated double lox recombination to embryonic stem (ES) cells to facilitate repeated knock-ins at a target locus, thus helping to assure correct temporal and spatial transgene expression in mice. Using homologous recombination, we inserted a double lox cassette a few nucleo-tides before the authentic ATG start of MHL-1, the gene coding for the major subunit of the asialoglycoprotein receptor. The cassette carries a marker gene bounded by heterospecific lox sites that cannot recombine with each other, but which can undergo recombination with like sites on an incoming double lox targeting vector. Cre-mediated replacement of the lox-delimited genomic segment at MHL-1 with targeting DNA occurs at a frequency three times that of random DNA integration and is sufficiently robust that correctly targeted ES colonies can be identified by PCR screening without relying on any drug selection procedure. Moreover, double lox recombination at the MHL-1 locus is not dependent on the presence or absence of a transcriptionally active promoter at the genomic target. The strategy and vectors described here are generally applicable to designing double lox targeted knock-ins at any locus in ES cells and should prove useful in more precise molecular engineering of the mouse genome.  (+info)

TY - JOUR. T1 - Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor. AU - Yang, Seung Hyun. AU - Heo, Dan. AU - Lee, Eugene. AU - Kim, Eunjung. AU - Lim, Eun Kyung. AU - Lee, Young Han. AU - Haam, Seungjoo. AU - Suh, Jin Suck. AU - Huh, Yong Min. AU - Yang, Jaemoon. AU - Park, Sahng Wook. PY - 2013/11/29. Y1 - 2013/11/29. N2 - Cancer cells can express specific biomarkers, such as cell membrane proteins and signaling factors. Thus, finding biomarkers and delivering diagnostic agents are important in the diagnosis of cancer. In this study, we investigated a biomarker imaging agent for the diagnosis of hepatic cancers. The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. The utility of the ASGPr-targetable imaging agent, galactosylated MFNP (G-MFNP) was assessed by ...
TY - JOUR. T1 - Asialoglycoprotein receptor phosphorylation and receptor-mediated endocytosis in hepatoma cells. Effect of phorbol esters. AU - Fallon, R. J.. AU - Schwartz, A. L.. PY - 1988/1/1. Y1 - 1988/1/1. N2 - The asialoglycoprotein (ASGP) receptor on Hep G2 cells undergoes constitutive recycling and ligand endocytosis in the presence of phorbol dibutyrate, at a 50% reduced rate relative to control cells (Fallon, R. J., and Schwartz, A. L. (1986) J. Biol. Chem. 261, 15081-15089). The relevance of receptor phosphorylation to these events was investigated by selective immunoprecipitation of surface receptors with polyclonal anti-human ASGP antiserum and pulse-chase labeling with [32P]orthophosphate to identify subcellular locations of initial receptor phosphorylation events as well as the eventual fate of phosphorylated receptor during recycling. The surface immunoprecipitation method recovers greater than 95% of surface ASGP receptors and only 5% or less of intracellular ...
Clone REA608 recognizes the human and rat asialoglycoprotein receptor 1 (ASGPR1) antigen, a single-pass type II membrane protein, also known as C-type lectin domain family 4 member H1 (CLEC4H1) or hepatic lectin H1 (HL-1). ASGPR1 is the major subunit of the asialoglycoprotein receptor (ASGPR) and is specifically expressed on the sinusoidal and basolateral hepatocellular membranes, but not on the bile canalicular membrane. It plays a role in the clearance of desialylated proteins from the serum through endocytosis and lysosomal degradation.Additional information: Clone REA608 displays negligible binding to Fc receptors. - Belgique
Asialoglycoprotein Receptor 1兔多克隆抗体(ab42488)可与大鼠, 人样本反应并经WB, ICC/IF实验严格验证,被2篇文献引用并得到2个独立的用户反馈。
1FIH: Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor.
1FIF: Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor.
A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates the endocytosis of serum glycoproteins.
Rabbit Polyclonal Anti-Asialoglycoprotein Receptor 2 Antibody. Validated: WB, IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
A more directed approach to the delivery of therapeutic oligonucleotides specifically to the liver has been to target the asialoglycoprotein receptor (ASGPR) using a suitable glycoconjugate. Indeed, ASGPR is the ideal target for delivery of therapeutic oligonucleotides to the liver since it combines tissue specificity, high expression levels and rapid internalization and turnover. The use of oligonucleotide glycoconjugates has led to significant advances in therapeutic delivery as evidenced by the work of Alnylam Pharmaceuticals which has developed multivalent N-acetylgalactosamine (GalNAc) conjugated siRNAs that bind at nanomolar levels to ASGPR (1). A similar strategy has been applied at Ionis Pharmaceuticals directed at the development of antisense oligonucleotide therapeutics (2 ...
A number of different families of proteins share a conserved domain which was first characterized in some animal lectins and which seem to function as a calcium-dependent carbohydrate-recognition domain [(PUBMED:3290208), (PUBMED:8341801)]. This domain, which is known as the C-type lectin domain (CTL) or as the carbohydrate-recognition domain (CRD), consists of about 110 to 130 residues. There are four cysteines which are perfectly conserved and involved in two disulfide bonds.. There are proteins with modules similar in overall structure to CRDs that serve functions other than sugar binding. Therefore, a more general term C-type lectin-like domain was introduced to refer to such domains, although both terms C-type lectin and C-type lectin-like are sometimes used interchangeably [(PUBMED:16336259)].. C-type lectins can be further divided into seven subgroups based on additional non-lectin domains and gene structure: (I) hyalectans, (II) asialoglycoprotein receptors, (III) collectins, (IV) ...
Carbohydrate-oligonucleotide conjugates have become increasingly important in recent years, particularly with regard to an increased uptake and cell availability of siRNA and antisense oligonucleotides. Of particular interest here is N-acetylgalactosamine (GalNAc), which can bind to the asialoglycoprotein receptor (ASGPR) in hepatocytes.. The following picture shows some examples of the attachment of carbohydrates (sugars) to oligonucleotides via different linker structures:. ...
Carbohydrate-oligonucleotide conjugates have become increasingly important in recent years, particularly with regard to an increased uptake and cell availability of siRNA and antisense oligonucleotides. Of particular interest here is N-acetylgalactosamine (GalNAc), which can bind to the asialoglycoprotein receptor (ASGPR) in hepatocytes.. The following picture shows some examples of the attachment of carbohydrates (sugars) to oligonucleotides via different linker structures:. ...
RESULTS: We found a rare noncoding 12-base-pair (bp) deletion (del12) in intron 4 of ASGR1, which encodes a subunit of the asialoglycoprotein receptor, a lectin that plays a role in the homeostasis of circulating glycoproteins. The del12 mutation activates a cryptic splice site, leading to a frameshift mutation and a premature stop codon that renders a truncated protein prone to degradation. Heterozygous carriers of the mutation (1 in 120 persons in our study population) had a lower level of non-HDL cholesterol than noncarriers, a difference of 15.3 mg per deciliter (0.40 mmol per liter) (P=1.0×10(-16)), and a lower risk of coronary artery disease (by 34%; 95% confidence interval, 21 to 45; P=4.0×10(-6)). In a larger set of sequenced samples from Icelanders, we found another loss-of-function ASGR1 variant (p.W158X, carried by 1 in 1850 persons) that was also associated with lower levels of non-HDL cholesterol (P=1.8×10(-3 ...
The incorporation of carbohydrate functional elements in the architecture of polycationic amphiphilic cyclodextrins (paCDs) provides glycosylated paCDs (pGaCDs) that form transfectious nanocomplexes (glycoCDplexes) with pDNA. In this study, we aimed at elucidating the internalization mechanisms at play and their incidence in transfection efficiency for glycoCDplexes formulated with 6-amino-6-deoxy-β-d-glucopyranosyl-appended pGaCDs in comparison with mannosylated and non-glycosylated congeners. Preliminary data showed a relatively high uptake of the 6-aminoglucosylated nanocomplexes by BNL-CL2 hepatocytes that correlated with a strong affinity towards the galactose-specific peanut agglutinin (PNA) lectin, suggesting that the galactose-binding asialoglycoprotein receptor at the surface of hepatocytes might be involved in glycoCDplex internalization. Transfection kinetics, internalization rates and protein expression data in BNL-CL2 ASGPR-expressing cells and COS-7 ASGPR-devoid epithelial cells ...
Acetyl-LDL (Ac-LDL) bound to transformed mouse macrophage J774 cells in a high affinity, saturable and specific manner. When cells were cultured for 24h in the presence of tunicamycin such that incorporation of N-linked sugars into protein but not protein synthesis itself was inhibited significantly, the binding characteristics of Ac-LDL to the cells were unaltered. In this respect the Ac-LDL receptor of J774 cells is similar to the asialoglycoprotein receptor of HepG2 cells. ...
Presentation of sialic acid (Sia) varies among different tissues and organs within each species, and between species. This diversity has biologically important consequences regarding the recognition of cells by xeno antibodies (Neu5Gc vs Neu5Ac). Sia also plays a central role in inflammation by influencing binding of the asialoglycoprotein receptor 1 (ASGR-1), Siglec-1 (Sialoadhesin), and cellular interactions mediated by the selectin, integrin, and galectin receptor families. This review will focus on what is known about basic Sia structure and function in association with xenotransplantation, how changes in sialylation may occur in this context (through desialylation or changes in sialyltransferases), and how this fundamental pathway modulates adhesive and cell activation pathways that appear to be particularly crucial to homeostasis and inflammation for xenografts ...
The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid…
Purified DNA encoding CAR receptors and the recombinant proteins expressed from such DNA are disclosed. The recombinant receptor polypeptides are used to identify CAR ligands and CAR receptor binding sites and are also used to produce therapeutics. Antibodies specific for CAR receptor polypeptides are also disclosed.
Practical information about Kigali airport, visa and health care information, the weather, public transport and the local currency.
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The number of Asialoglycoprotein (ASGP) receptors on the hepatocytes of patients with liver disease is reduced and is thus considered a good indicator for the evaluation of liver function. ASGP receptor-targeted radiopharmaceuticals permit a non-invasive way to evaluate total and regional hepatic function and hepatic functional reserve visually and quantitatively. Over the past three decades, a variety of ASGP receptor-targeted probes have been developed with different molecular backbones (albumin, polymer, small-molecular-weight ligand), different glycol-residues (galactose, lactose, N-acetyl-galactosamine) and different chelating systems suitable for radiolabeling with SPECT isotopes (Tc-99m, In-111, Ga-67, (131)/I-125, Sm-153) and PET isotopes (Ga-68, F-18). In this review, we present an overview of ASGP receptor-targeted radiopharmaceuticals, discuss their chemistry, biodistribution, catabolism and challenge as well as application for measurement of liver function ...
The major and minor forms of the asialoglycoprotein receptor (ASGP-R), designated in the rat as RHL-1 and RHL-2/3, respectively, have traditionally been considered to be expressed exclusively in hepatic parenchymal cells. Northern blot analysis now demonstrates that rat and mouse testis express a receptor similar to the RHL-1 ASGP-R but not the RHL-2/3 receptor. In situ hybridization studies demonstrate that late-stage mouse and rat spermatids are the testicular cells that express the RHL-1 ASGP-R. The rat spermatid RHL-1 receptor has functional binding capability, with a KD of approximately 1.4 x 10(-8) M, and similar characteristics, compared with the hepatic RHL-1 receptor, regarding ligand binding specificity and ion dependence. These findings have major implications for therapeutic procedures attempting to target cytotoxic agents or DNA to hepatocytes using the ASGP-R. In addition, these findings demonstrate that late-stage spermatids are transcriptionally active and suggest that the RHL-1 ...
Formulated Receptor Polypeptides and Related Methods | AGENT FOR PROMOTING SUBSTANCE INCORPORATION IN INTESTINAL TRACT | Materials and Methods of Treating Dyslipidemia | FORMULATIONS OF VANCOMYCIN | NOVEL ENTERIC COMBINATION THERAPY |
Dave Weigel writes the smartest thing Ive read about the Sarah Palin phenomenon in some time. The problem is that Palin has put the political press in a submissive position, one in which the only information it prints about her...
Question - Dark spots and acne marks removal. Ask a Doctor about diagnosis, treatment and medication for Acne vulgaris, Ask a Dermatologist
TY - JOUR. T1 - Long-term reduction of jaundice in Gunn rats by nonviral liver-targeted delivery of Sleeping Beauty transposon. AU - Wang, Xia. AU - Sarkar, Debi P.. AU - Mani, Prashant. AU - Steer, Clifford J.. AU - Chen, Yong. AU - Guha, Chandan. AU - Chandrasekhar, Voshavar. AU - Chaudhuri, Arabinda. AU - Roy-Chowdhury, Namita. AU - Kren, Betsy T.. AU - Roy-Chowdhury, Jayanta. N1 - Copyright: Copyright 2010 Elsevier B.V., All rights reserved.. PY - 2009. Y1 - 2009. N2 - Asialoglycoprotein receptor (ASGPR)-mediated endocytosis has been used to target genes to hepatocytes in vivo. However, the level and duration of transgene expression have been low because of lysosomal translocation and degradation of the DNA and lack of its integration into the host genome. In this study we packaged the DNA of interest in proteoliposomes containing the fusogenic galactose-terminated F-glycoprotein of the Sendai virus (FPL) for targeted delivery to hepatocytes. After the FPL binds to ASGPR on the hepatocyte ...
The glycine receptor of rat spinal cord is an oligomeric membrane glycoprotein of molecular mass 250,000 daltons that contains three polypeptides of 48,000, 58,000, and 93,000 daltons. Monoclonal antibodies (mAbs) were prepared against the affinity-purified glycine receptor protein by using 125I-labeled receptor preparations for the detection of positive hybrids. From nine monoclonal antibodies obtained, six recognized denatured receptor polypeptides blotted to nitrocellulose paper. Two of these antibodies bound to more than one glycine receptor polypeptide: mAb GlyR 4a stained the 48,000- and 58,000-dalton polypeptides, and mAb GlyR 7a stained the 48,000- and 93,000-dalton polypeptides. Common antigenic determinants thus are shared by the different subunits of the glycine receptor. Complementary results were obtained by peptide mapping of 125I-labeled glycine receptor polypeptides with various proteases. A set of peptide fragments of the same apparent molecular mass was produced from the ...
0041]It is presently preferred to isolate E1 and/or E2 asialoglycoproteins from crude cell lysates by incubation with an immobilized mannose-binding protein, particularly a lectin such as ConA or GNA. Cells are lysed, e.g., by mechanical disruption in a hypotonic buffer followed by centrifugation to prepare a post-nuclear lysate, and further centrifuged to obtain a crude microsomal membrane fraction. The crude membrane fraction is subsequently solubilized in a buffer containing a detergent, such as Triton X-100, NP40, or the like. This detergent extract is further clarified of insoluble particulates by centrifugation, and the resulting clarified lysate incubated in a chromatography column comprising an immobilized mannose-binding protein, preferably GNA bound to a solid support such as agarose or Sepharose® for a period of time sufficient for binding, typically 16 to 20 hours. The suspension is then applied to the column until E1/E2 begins to appear in the eluent, then incubated in the column ...
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French Catholic philosopher Jacques Maritain once wrote that Americans are the least materialist among the modern peoples which have attained the industrial stage.. Drawing on this sentiment, George Weigel argues that although materialism may reign in America more than it once did, there remains a link between money-making and idealism in these United States that is distinctive, and perhaps even unique.. Pointing to President Calvin Coolidge (no fan of materialism), Weigel emphasizes that Coolidges famous line-the chief business of the American people is business-shouldnt be taken by itself. For Coolidge, and for most Americans (even today), promoting the dignity-conferring effects of business is part of a larger, deeper idealism.. As Weigel explains:. As for wealth, consider Silent Cals remarks at the end of the same speech: We make no concealment of the fact that we want wealth, but there are many other things that we want very much more. We want peace and honor, and that charity ...
Welcome to Biology 1510 for Fall 2017! Select course readings from the menus above by Module: Evolution Ecology Molecules and Metabolism Genetics Integrative Human Health Lecture materials can be downloaded from t-square Instructions for the group video project will be … Continue reading →. ...
K. Schneeberger, Hagmann, J., Ossowski, S., Warthmann, N., Gesing, S., Kohlbacher, O., and Weigel, D., Simultaneous alignment of short reads against multiple genomes, Genome Biol, vol. 10, p. R98, 2009. ...
Hodotermes is a genus that contains two species of African harvester termites known as H. mossambicus and H. erithreensis. The collective range of both species extends from some areas of South Africa through the savannas in East Africa to Palaearctic North Africa. Both species are subterraneous, nesting under the ground, but the nests of H. mossambicus can contain several sphere-shaped hives, and long tunnels connect the rooms of each nest. It is typical for all harvester termites to have compound eyes are serrated edges on the mandibles.. Breeding members of the Hodotermes genus, known as alates, emerge from nests in the summer months after evening hours. Once the alates land, they break off their wings and begin searching for suitable mates. When a pair has finished courtship, they will find a new area to start dig a nest and become the king and queen of a new colony. Eggs are laid about a week after breeding and soon after the resulting workers will begin to tend to new eggs and the nest. ...
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and can regulate neurotransmitter release. This receptor can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system. [provided by RefSeq, Jul 2008]
The obligate human pathogen, Neisseria gonorrhoeae, has evolved mechanisms to manipulate the apoptotic machinery in human epithelial cells in favor of host niche adaptation. In the present research, I investigated the apoptotic effect of N. gonorrhoeae on transduced human urethral epithelial cells (THUEC) and the underlying mechanism of apoptosis modulation. Flow cytometric analysis showed that gonococcal infection conferred an anti-apoptotic effect in early infection, but induced apoptosis during prolonged infection periods in THUEC. Intracellular gonococci were required to provide the anti-apoptotic effect. Furthermore, immunoblotting analysis of the mitogen-activated protein kinase (MAPK) pathways mapped the apoptosis resistance to a signaling cascade involving epidermal growth factor receptor (EGFR), extracellular signal-regulated kinases (ERK), and Bim/Bad, in which the inhibition of ERK activation by N. gonorrhoeae contributed to the anti-apoptotic effect on THUEC. A N. gonorrhoeae minD ...
Exactly. News organizations dont hire conservatives. Therefore, conservatives dont seek careers in journalism and instead become lawyers or accountants or stock brokers or whatever.. Ergo, none of the people making decisions in MSM newsrooms are conservatives. Where set A = employees of news organizations and set B = conservatives, we see that as the closer set AB approaches to zero, the more likely journalists are to view conservatives as The Other, and vice-versa.. If you think such a situation is a good thing, then youre obviously congratulating yourself about Dave Weigels downfall, and you dont want to hear the explanation of my disagreement.. UPDATE VI: Not to say there isnt an upside: Ezra Klein has deep-sixed Journolist as a result of the Weigel affair. Little Miss Attila says the list had become blackmail fodder.. I spoke to Weigel by phone this afternoon. Hes fine, and is currently deciding which of three or four job offers to accept. He admits that he succumbed to hubris, ...
Chao, D. (Creator), Borevitz, J. O. (Creator), Atwell, S. (Creator), Huang, Y. S. (Creator), Vilhjalmsson, B. J. (Creator), Willems, G. (Creator), Horton, M. (Creator), Li, Y. (Creator), Meng, D. (Creator), Platt, A. (Creator), Tarone, A. M. (Creator), Hu, T. T. (Creator), Jiang, R. (Creator), Muliyati, N. W. (Creator), Zhang, X. (Creator), Amer, M. A. (Creator), Baxter, I. (Creator), Brachi, B. (Creator), Chory, J. (Creator), Dean, C. (Creator), Debieu, M. (Creator), de Meaux, J. (Creator), Ecker, J. R. (Creator), Faure, N. (Creator), Kniskern, J. M. (Creator), Jones, J. D. G. (Creator), Michael, T. (Creator), Nemri, A. (Creator), Roux, F. (Creator), Salt, D. E. (Creator), Tang, C. (Creator), Todesco, M. (Creator), Traw, M. B. (Creator), Weigel, D. (Creator), Marjoram, P. (Creator), Bergelson, J. (Creator), Nordborg, M. (Creator), Silva, A. (Creator), GEO, 31 Jul 2012. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39679 and 2 more links, ...
Research project, Figurations of the Martyr in Near Eastern and European Literature, Angelika Neuwirth, Sigrid Weigel, Zentrum für Literatur- und …
From the international download user centered agile methods synthesis lectures on of Baths narrative purpose to the Millers modern, whole manuscript, these downloads can contain incorporated as a economy of fourteenth-century London. 1343-1400) was an deviant reform, master, practice, credit and engine, best provided as the presentation of The Canterbury Tales. Chaucer has Subscribed as implementing the revealing quart to Help the educational field of the wide medical policy.
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified ...
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David Weigel has an article on how things have changed for those who talked about rebranding the Republicans after their 2006 and 2008 loss. Some Republicans were looking at ways the party should change direction and possibly take some less extreme position son some issues. At the moment, with Republicans appearing on the brink of doing well in the off-year elections, it looks like such rebranding might not have been needed. Republicans believe they are doing fine by sticking to their old views or moving even further to the right.. That is a rather short sighted view of the situation. In a two party system one party can have some victories even when on the wrong side of the issues. Many people are bound to vote for the party out of power when the economy is bad. Republicans also have an advantage this year because Democrats are trying to defend a large number of seats which have traditionally been held by Republicans.. Under these conditions it would come as no surprise that the Republicans ...
George Weigel is Americans leading lay authority on the Roman Catholic Church, a biographer of John Paull II and benedict XVI, and one of the most sought-
Radiation inactivation and sedimentation equilibrium analysis were used to determine the functional and physical size of the chicken hepatic membrane receptor that binds N-acetylglucosamine-terminated glycoproteins. Purified plasma membranes from chicken liver were irradiated with high energy electr …
The long read: For 25 years, invoking this vague and ever-shifting nemesis has been a favourite tactic of the right - and Donald Trumps victory is its greatest triumph
https://www.technologyreview.com/s/601495/amgen-finds-anti-heart-attack-gene/ http://www.nejm.org/doi/full/10.1056/NEJMoa1508419 Variant ASGR1 Associated with a Reduced Risk of Coronary Artery Disease In ~50,000 patients studied, two insertion/deletion variants were found in the paper cited above, amidst a region of seven linked SNPs. Each of these variants reduces risk for myocardial infarction by about a third (hazard ratio, 0.64, CI: 0.64 - 0.80, p=8.5×10e−5) and led to a life span that was on average 1.5 years longer than that for noncarriers (CI:, 0.2 to 2.8, p=0.02). The two variants are: ...
DNA molecules complexed with an asialoglycoprotein-polycation conjugate, consisting of asialoorosomucoid (ASOR) coupled to poly-l-lysine, can enter hepatocytes which bear receptors for ASOR. We used this receptor-mediated DNA delivery system to deliver plasmid DNA encoding glycoprotein D (gD) of herpes simplex virus type 1 to ASOR-positive cells. Maximum expression of gD protein was seen at 3 days after injection of this preparation in approximately 13% of cells from BALB/c mice [hepatocytes from mice injected intravenously (i.v.) or peritoneal exudate cells from mice injected intraperitoneally (i.p.)]. In comparison with mice injected with either the plasmid vector alone or the gD-containing plasmid uncomplexed to ASOR, mice immunized with gD-containing plasmid complexed with ASOR-poly-l-lysine induced marked antigen-specific CTL responses. BALB/c mice immunized with gD-DNA developed a T-cell-mediated CTL response against target cells expressing gD and MHC class II glycoproteins, but not against cells
TY - JOUR. T1 - Receptor-mediated uptake of human α1-acid glycoprotein into liver parenchymal cells in mice. AU - Matsumoto, Kazuaki. AU - Nishi, Koji. AU - Kikuchi, Mari. AU - Watanabe, Hiroshi. AU - Nakajou, Keisuke. AU - Komori, Hisakazu. AU - Kadowaki, Daisuke. AU - Suenaga, Ayaka. AU - Maruyama, Toru. AU - Otagiri, Masaki. N1 - Funding Information: Acknowledgments: This research was supported Grants-in-aid in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) (18590035 and 20390161).. PY - 2010. Y1 - 2010. N2 - Human α1-acid glycoprotein (AGP), a serum glycoprotein, is thought to have anti-inflammatory effects by a mechanism that is not well understood. In this study, we investigated the pharmacokinetics of AGP in mice using enzymatically modified AGP (AGP with the sialic acids removed, asialo-AGP, and with both sialic acids and galactose removed, agalacto-AGP). It was observed that AGP was eliminated from the circulation slowly, and ...
We already know Weigel is our enemy, by his own words.. ODonnell wont even rule out running as an independent if she loses the primary. She lies about her record and finances, is paying her rent with campaign money, and sold her house to her campaign lawyer to avoid foreclosure.. Castles a RINO, but ODonnell is a lying crook. The latest Rasmussen poll shows Castle beating the Democrat by double digits, and ODonnell losing by double digits.. What is it about lying, crooked losers like ODonnell and Hayworth that attract some conservatives to support them? Have you no ethical standards at all?. ...
Shop IgA receptor ELISA Kit, Recombinant Protein and IgA receptor Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Weiss, P; Ashwell, G (1989). "The asialoglycoprotein receptor: properties and modulation by ligand". Progress in Clinical and ...
The asialoglycoprotein receptors that bind Sendai virus. and serve as SeV cell entry receptors are highly expressed in liver ... Three SeV receptors are represented by molecules that are clusters of differentiation. Some SeV receptors are overexpressed in ... Stockert, R. J. (July 1995). "The asialoglycoprotein receptor: relationships between structure, function, and expression". ... entry by means of the asialoglycoprotein receptor of a Sendai virus mutant lacking its attachment protein". Proceedings of the ...
Clearance of IgA is mediated at least in part by asialoglycoprotein receptors, which recognizes galactose-terminating IgA N- ... Proteolysis of the receptor occurs, and the dimeric IgA molecule, along with a portion of the receptor known as the secretory ... and mucosal epithelial cells that express an immunoglobulin receptor called the polymeric Ig receptor (pIgR). Polymeric IgA ( ... see B-cell receptor) It is also possible to distinguish forms of IgA based upon their location - serum IgA vs. secretory IgA. ...
... where it binds to the asialoglycoprotein receptors on hepatocytes. Galactosamine Globoside (N-Acetylglucosamine) GlcNAc Donald ...
... residues results in rapid clearance of the glycoproteins from the circulation through hepatocyte asialoglycoprotein receptors ... Asialoglycoproteins at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... If terminal sialic acid residues are removed from glycoproteins, the resulting proteins are known as asialoglycoproteins. The ...
Stockert, R. J. (1995-07-01). "The asialoglycoprotein receptor: relationships between structure, function, and expression". ... Androgen receptors Calcitriol receptors Corticotropin-releasing hormone receptor 1 Corticotropin releasing hormone receptor 2 ... hormone receptors Glucagon receptors Gonadotropin receptors Gonadotropin-releasing hormone receptors Growth hormone receptors ... Insulin receptor Luteinizing hormone Progesterone receptors Retinoid receptors Somatostatin receptors Thyroid hormone receptors ...
The asialoglycoprotein receptors are lectins which bind asialoglycoprotein and glycoproteins from which a sialic acid has been ... The asialoglycoprotein receptor has been demonstrated to have high expression on the surface of hepatocytes [1], several human ... Asialoglycoprotein+receptor at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... Lactobionic acid has been used as a targeting moiety for drug delivery to cells expressing asialoglycoprotein receptors. ...
... to discover that a certain receptor in a human's liver is able to recognize a specific glycoprotein called asialoglycoprotein. ... He is a member of the National Academy of Science for his work with Anatol Morell in isolating the first cell receptor. Ashwell ... Ashwell explained that he was not specifically looking for the asialoglycoprotein when he found it. Ashwell died on June 27, ... and discovered one of the earliest known carbohydrate receptors. They were able to devise a labeling procedure which allowed ...
Another potential characterization of the CLRs can be into mannose receptors and asialoglycoprotein receptors. The mannose ... mannose versus asialoglycoprotein) is a bit misleading as these the asialoglycoprotein receptors are not necessarily galactose ... and C-type lectin receptors (CLRs). Cytoplasmic PRRs include NOD-like receptors (NLRs) and RIG-I-like receptors (RLRs). PRRs ... For more details, see NOD-like receptor. The NOD-like receptors (NLRs) are cytoplasmic proteins, which recognize bacterial ...
"In vitro binding of the asialoglycoprotein receptor to the beta adaptin of plasma membrane coated vesicles". The EMBO Journal. ... Schleiff E, Shore GC, Goping IS (March 1997). "Human mitochondrial import receptor, Tom20p. Use of glutathione to reveal ...
Asialoglycoprotein receptor. *Tumor necrosis factor receptor. *Immunoglobulin superfamily. *N-Acetylglucosamine receptor. * ... Prostaglandin receptors or prostanoid receptors represent a sub-class of cell surface membrane receptors that are regarded as ... All of the prostanoid receptors are G protein-coupled receptors belonging to the Subfamily A14 of the rhodopsin-like receptor ... There are 9 established prostanoid receptors. The following table gives these receptors: a) full name; b) shortened names; c) ...
Aryl hydrocarbon receptor repressor. *Aryl sulfotransferase. *Asialoglycoprotein receptor. *Aspartate carbamoyltransferase. * ...
Asialoglycoprotein receptor. *Tumor necrosis factor receptor. *Immunoglobulin superfamily. *N-Acetylglucosamine receptor. * ... GABA receptors: GABA-A, GABA-C. GABA. Cl− , HCO−3 [11]. Glutamate receptors: NMDA receptor, AMPA receptor, and Kainate receptor ... toll-like receptors (TLRs), killer activated and killer inhibitor receptors (KARs and KIRs), complement receptors, Fc receptors ... receptors, G protein-linked (metabotropic) hormone receptors, and enzyme-linked hormone receptors.[1] Intracellular receptors ...
Clearance of IgA is mediated at least in part by asialoglycoprotein receptors, which recognizes galactose-terminating IgA N- ... Proteolysis of the receptor occurs, and the dimeric IgA molecule, along with a portion of the receptor known as the secretory ... and mucosal epithelial cells that express an immunoglobulin receptor called the polymeric Ig receptor (pIgR). pIgA is released ... Both IgA1 and IgA2 can be in membrane-bound form.[12] (see B-cell receptor) ...
Asialoglycoprotein receptor. *Tumor necrosis factor receptor. *Immunoglobulin superfamily. *N-Acetylglucosamine receptor. * ... Rats rely heavily on olfactory sensory input from olfactory receptors for behavioral attitudes.[28] Studies show that bilateral ... The olfactory tubercle has been shown to be concerned primarily with the reception of sensory impulses from olfactory receptors ... receptor D3 is abundant in this two areas [6]). In addition, the OT contains tightly packed cell clusters known as the islands ...
Asialoglycoprotein receptor. *Tumor necrosis factor receptor. *Immunoglobulin superfamily. *N-Acetylglucosamine receptor. * ... An example of membrane receptors. *Ligands, located outside the cell. *Ligands connect to specific receptor proteins based on ... The receptor releases a messenger once the ligand has connected to the receptor. ... Wikipedia:MeSH D12.776#MeSH D12.776.543.750 - receptors.2C cell surface. അവലംബം[തിരുത്തുക]. *↑ Hall, JE (2016). Guyton and Hall ...
Receptor/signaling modulators. Signaling peptide/protein receptor modulators. Growth factor receptor modulators. ... 4-1BB is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily, expressed on activated T Lymphocytes.[1 ... Asialoglycoprotein. *Avidin. *B-cell activating factor. *4-1BB ligand. *Cholesterylester transfer protein ...
Miraculin, is a glycoprotein extracted from Synsepalum dulcificum a berry which alters human tongue receptors to recognize sour ... Asialoglycoprotein. *Avidin. *B-cell activating factor. *4-1BB ligand. *Cholesterylester transfer protein ...
... receptor and intracellular accumulation of cyclic AMP. High AGP concentrations, however, inhibited TSH signalling.[5][6] ... and its glycoforms on the function of human thyrocytes and CHO cells transfected with the human TSH receptor". Molecular and ... Asialoglycoprotein. *Avidin. *B-cell activating factor. *4-1BB ligand. *Cholesterylester transfer protein ...
cytokine receptor activity. Cellular component. • integral component of membrane. • Golgi apparatus. • macrophage migration ... CD44 is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix ... Aruffo A, Stamenkovic I, Melnick M, Underhill C, Seed B (Jul 1990). "CD44 is the principal cell surface receptor for ... "bone homing receptor", directing migration of human hematopoietic stem cells and mesenchymal stem cells to bone marrow.[11] Ex ...
The asialoglycoprotein receptors are lectins which bind asialoglycoprotein and glycoproteins from which a sialic acid has been ... The asialoglycoprotein receptor has been demonstrated to have high expression on the surface of hepatocytes [1], several human ... Asialoglycoprotein+receptor at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... Lactobionic acid has been used as a targeting moiety for drug delivery to cells expressing asialoglycoprotein receptors. ...
Rabbit polyclonal Asialoglycoprotein Receptor 1/HL-1 antibody. Validated in WB, IHC and tested in Human. Cited in 1 publication ... Anti-Asialoglycoprotein Receptor 1/HL-1 antibody. See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies. ... Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab49355) at 0.6 µg/ml + Fetal liver lysate at 10 µg with 5% skim milk in PBS. ... Protein - Recombinant Human Asialoglycoprotein Receptor 1/HL-1 (ab151893) SDS-PAGE, HPLC ...
What is asialoglycoprotein receptor? Meaning of asialoglycoprotein receptor medical term. What does asialoglycoprotein receptor ... Looking for online definition of asialoglycoprotein receptor in the Medical Dictionary? asialoglycoprotein receptor explanation ... This study evaluated the usefulness of asialoglycoprotein receptor scintigraphy with [sup.. Asialoglycoprotein receptor ... asialoglycoprotein receptor. Also found in: Wikipedia. a·si·a·lo·gly·co·pro·tein re·cep·tor. a surface receptor found in ...
A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates ... Receptor, Hepatic Asialoglycoprotein; Receptors, Asialoglycoprotein; Receptors, Asialoorosomucoid; Asialoglycoprotein Receptors ... Hepatic Asialoglycoprotein Receptor; Hepatic Lectin; Liver Carbohydrate-Binding Protein; Receptor, Asialoglycoprotein; Receptor ... Asialoglycoprotein Receptor. Subscribe to New Research on Asialoglycoprotein Receptor A C-type lectin that is a cell surface ...
Rabbit Polyclonal Anti-Asialoglycoprotein Receptor 2 Antibody. Validated: WB, IHC, IHC-P. Tested Reactivity: Human. 100% ... Additional Asialoglycoprotein Receptor 2 Products. Asialoglycoprotein Receptor 2 NBP1-52958 * Asialoglycoprotein Receptor 2 ... Home » Asialoglycoprotein Receptor 2 » Asialoglycoprotein Receptor 2 Antibodies » Asialoglycoprotein Receptor 2 Antibody ... Blogs on Asialoglycoprotein Receptor 2. There are no specific blogs for Asialoglycoprotein Receptor 2, but you can read our ...
Effect of chronic ethanol administration on total asialoglycoprotein receptor content and intracellular processing of ... In addition to a decreased number of intracellular receptors, an impairment in intracellular processing of receptor-ligand ... These results indicate that chronic ethanol administration decreases ligand binding due to a decreased number of receptors and ... Ethanol administration for 5-7 weeks decreased intracellular ASOR receptor content by 40%, a result which was confirmed by ...
Kaninchen Polyklonal Asialoglycoprotein Receptor 2 Antikörper für WB. Order anti-Asialoglycoprotein Receptor 2 Antikörper ... Background: This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays ... Target Details Asialoglycoprotein Receptor 2 Bilder Handhabung Anwendungsinformationen zurück nach oben Target Details ... Produktdetails anti-Asialoglycoprotein Receptor 2 Antikörper Bilder Handhabung Anwendungsinformationen Antigendetails zurück ...
Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor. ... Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor ... Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor ... Hodgkins Cell Lectin: An Ectosialyltransferase and Lymphocyte Agglutinant Related to the Hepatic Asialoglycoprotein Receptor ...
... receptor is an abundant hepatocyte-specific receptor involved in receptor-mediated endocytosis. This receptors abundance and ... The asialoglycoprotein (ASGP) receptor is an abundant hepatocyte-specific receptor involved in receptor-mediated endocytosis. ... Thus, receptor-mediated endocytosis by the ASGP receptor could represent a novel molecular mechanism that is responsible for ... Briefly, ASGP receptor-deficient (RD) mice and wild-type (WT) mice were injected with 1 ml/kg body weight of CCl4. In the ...
Effects of vanadate on receptor-mediated endocytosis of asialoglycoproteins in isolated rat hepatocytes TROND BERG TROND BERG ... TROND BERG, OLA GUDMUNDSEN, GRETE M. KINDBERG; Effects of vanadate on receptor-mediated endocytosis of asialoglycoproteins in ...
The major subunit of the human asialoglycoprotein receptor contains signals for efficient endocytosis and specific basolateral ... The major subunit of the human asialoglycoprotein receptor contains signals for efficient endocytosis and specific basolateral ... Related signals for endocytosis and basolateral sorting of the asialoglycoprotein receptor. Journal of Biological Chemistry, ... The kinetics of appearance of newly synthesized wild-type and mutant receptor protein at the apical and basolateral surfaces ...
The human asialoglycoprotein receptor, an endocytic transport receptor of the basolateral surface of hepatocytes, is a hetero- ... The human asialoglycoprotein receptor, an endocytic transport receptor of the basolateral surface of hepatocytes, is a hetero- ... Fuhrer, C; Geffen, I; Huggel, K; Spiess, M (1994). The two subunits of the asialoglycoprotein receptor contain different ... Thus, whereas the endocytosis signal of the hetero-oligomeric asialoglycoprotein receptor resides exclusively in subunit H1, ...
Late-stage spermatids are characterized by expression of the "liver-specific" asialoglycoprotein receptor, RHL-1.. B E Huber ... Late-stage spermatids are characterized by expression of the "liver-specific" asialoglycoprotein receptor, RHL-1.. B E Huber ... Late-stage spermatids are characterized by expression of the "liver-specific" asialoglycoprotein receptor, RHL-1.. B E Huber ... The major and minor forms of the asialoglycoprotein receptor (ASGP-R), designated in the rat as RHL-1 and RHL-2/3, respectively ...
Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ... Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ... Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ... Apparent lack of second messenger involvement in receptor-mediated endocytosis of asialoglycoproteins by isolated rat ...
When the COOH terminus of the rat liver receptor is aligned with the corresponding portions of the chicken liver receptor, the ... Sequence homology between residues 50-79 and residues 121-150 of the rat receptor suggests that the additional length of this ... The overall arrangement of the polypeptide is similar to the chicken receptor for asialoagalactoglycoproteins; it consists of ... there must be at least two genes coding for receptor polypeptides. RHL-2 and RHL-3 may differ in their extent of ...
Asialoglycoprotein receptor 2 (Asgr2) Recombinant Protein-NP_001300854.1 (MBS7053730) product datasheet at MyBioSource, ... "Impaired receptor-mediated endocytosis by the asialoglycoprotein receptor in ethanol-fed mice: implications for studying the ... This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical ... The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by ...
A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates ... Asialoglycoprotein Receptor. Known as: Receptor, Asialoglycoprotein, ASGP-R, Asialoorosomucoid Receptors Expand. A C-type ... Intracellular site of asialoglycoprotein receptor-ligand uncoupling: Double-label immunoelectron microscopy during receptor- ... The asialoglycoprotein receptor clears glycoconjugates terminating with sialic acidα2,6GalNAc. *E. I. Park, Yiling Mi, C. ...
study of their receptor specificity (hepatic ASGP receptor versus galactosespecific receptors on the surfaces of other cells) ... The asialoglycoprotein-receptor (ASGP-R) is a C-type lectin predominantly expressed on the sinusoidal surface of mammalian ... Targeting hepatocytes via the asialoglycoprotein-receptor. 2010, PhD Thesis, University of Basel, Faculty of Science. ... to the receptor with known galactose-derived compounds. In this assay, the purified human H1-CRD of the ASGP-R expressed in E. ...
Asialoglycoprotein Receptor 1兔多克隆抗体(ab42488)可与大鼠, 人样本反应并经WB, ICC/IF实验 ... Anti-Asialoglycoprotein Receptor 1抗体. 参阅全部 Asialoglycoprotein Receptor 1 一抗. ... Anti-Asialoglycoprotein Receptor 1 antibody (ab42488) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at ... Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1 antibody (ab42488)This image is
title = "Tyrosine phosphorylation of the asialoglycoprotein receptor",. abstract = "The asialoglycoprotein (ASGP) receptor ... Tyrosine phosphorylation of the asialoglycoprotein receptor. / Fallon, R. J.. In: Journal of Biological Chemistry, Vol. 265, No ... Fallon, R. J. (1990). Tyrosine phosphorylation of the asialoglycoprotein receptor. Journal of Biological Chemistry, 265(6), ... Fallon, R. J. / Tyrosine phosphorylation of the asialoglycoprotein receptor. In: Journal of Biological Chemistry. 1990 ; Vol. ...
Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor Mcvicker, Benita L. Omaha VA Medical Center, ... Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor. Mcvicker, Benita L. / Omaha VA Medical Center ... Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor. Mcvicker, Benita L. / Omaha VA Medical Center ... Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor. Mcvicker, Benita L. / Omaha VA Medical Center ...
Fingerprint Dive into the research topics of Tyrosine phosphorylation of the asialoglycoprotein receptor. Together they form ...
Weiss, P; Ashwell, G (1989). "The asialoglycoprotein receptor: properties and modulation by ligand". Progress in Clinical and ...
The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent ... The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent ... The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent ... The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent ...
Regulation of asialoglycoprotein receptor expression in the proliferative state of hepatocytes. Shinichi Hirose, Hirohiko Ise, ... Hirose S, Ise H, Uchiyama M, Cho CS, Akaike T. Regulation of asialoglycoprotein receptor expression in the proliferative state ... Hirose, S., Ise, H., Uchiyama, M., Cho, C. S., & Akaike, T. (2001). Regulation of asialoglycoprotein receptor expression in the ... Regulation of asialoglycoprotein receptor expression in the proliferative state of hepatocytes. / Hirose, Shinichi; Ise, ...
The asialoglycoprotein receptor (ASGPR) is considered one of the target antigens in the pathogenesis of autoimmune chronic ... The asialoglycoprotein receptor (ASGPR) is considered one of the target antigens in the pathogenesis of autoimmune chronic ... The asialoglycoprotein receptor (ASGPR) is considered one of the target antigens in the pathogenesis of autoimmune chronic ... The asialoglycoprotein receptor (ASGPR) is considered one of the target antigens in the pathogenesis of autoimmune chronic ...
Erratum to: Lactosaminated mesoporous silica nanoparticles for asialoglycoprotein receptor targeted anticancer drug delivery. * ... Lactosaminated mesoporous silica nanoparticles for asialoglycoprotein receptor targeted anticancer drug delivery. J Nanobiotech ...
... and the asialoglycoprotein receptor (ASGPR). The ASGPR and the ManR are carbohydrate-specific, endocytic receptors expressed by ... Stefanich EG, Ren S, Danilenko DM, Lim A, Song A, Iyer S, Fielder PJ (2008) Evidence for an asialoglycoprotein receptor on ... Mi Y, Lin A, Fiete D, Steirer L, Baenziger JU (2014) Modulation of mannose and asialoglycoprotein receptor expression ... This may occur, for example, when targeting a receptor on a solid tumor which is poorly accessible, and the receptor is also ...
The purpose of this study was to define the distribution of the asialoglycoprotein receptor (ASGP-R) main peptide, rat hepatic ... The purpose of this study was to define the distribution of the asialoglycoprotein receptor (ASGP-R) main peptide, rat hepatic ... The lobular expression of the rat asialoglycoprotein receptor is regulated at post-transcriptional level. ... The lobular expression of the rat asialoglycoprotein receptor is regulated at post-transcriptional level ...
Asialoglycoprotein Receptor 1) ELISA Kit based in Delhi, India ... Rat ASGR1(Asialoglycoprotein Receptor 1) ELISA Kit Rat ASGR1( ... Rat ASGR1(Asialoglycoprotein Receptor 1) ELISA Kit Synonyms:ASGR1. Intended use. This ELISA kit applies to the in vitro ... Rat AIP(Aryl Hydrocarbon Receptor Interacting Protein) ELISA Kit. *Rat ARNT2(Aryl Hydrocarbon Receptor Nuclear Translocator 2) ... Rat RANκ(Receptor Activator of Nuclear Factor Kappa B) ELISA Kit ... Rat ASGR1(Asialoglycoprotein Receptor 1) ELISA Kit. *Rat AST( ...
  • Further, iLNPs were engineered with the carbohydrate N-acetylgalactosamine (GalNAc) to achieve targeting to the asialoglycoprotein receptor (ASGPR) as an alternative targeting strategy for the hepatic delivery of RNAi therapeutics. (thefreedictionary.com)
  • The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. (elsevier.com)
  • The asialoglycoprotein receptor (ASGPR) is considered one of the target antigens in the pathogenesis of autoimmune chronic active hepatitis (AIH). (elsevier.com)
  • The purpose of this research was to recognize the induction and pathogenic potential of autoantibodies against a liver-specific asialoglycoprotein receptor (ASGPR) in the course of hepadnavirus infection. (mun.ca)
  • Western blot analyses showed that WHV-induced anti-ASGPR autoantibodies recognized both 40- and 47-kDa subunits of wASGPR and rASGPR, implying that both receptor polypeptides may serve as targets for the pathogenic immune reactions. (mun.ca)
  • These findings indicate that virus-induced autoantibodies against hepatic ASGPR have the potential to directly contribute to liver injury and to the distortion of the hepatocyte clearance of asialoglycoproteins in viral hepatitis. (mun.ca)
  • Likewise, asialoglycoprotein receptor (ASGPR) was up-regulated in UCMSCs upon differentiation. (sigmaaldrich.com)
  • Lactoferrin (Lf) is a potential-targeting ligand for hepatocellular carcinoma (HCC) cells because of its specific binding with asialoglycoprotein receptor (ASGPR). (dovepress.com)
  • Asialoglycoprotein receptor (ASGPR) as target autoantigen in liver autoimmunity: Lost and found. (biomedsearch.com)
  • The Ang receptor binding domains (RBD) were cloned linked to the asialoglycoprotein receptor (ASGPR) transmembrane domain. (bl.uk)
  • ASGPR1 is the major subunit of the asialoglycoprotein receptor (ASGPR) and is specifically expressed on the sinusoidal and basolateral hepatocellular membranes, but not on the bile canalicular membrane. (miltenyibiotec.com)
  • The asialoglycoprotein receptor has been demonstrated to have high expression on the surface of hepatocytes [1], several human carcinoma cell lines[2] and liver cancers. (wikipedia.org)
  • and,aA alternatively, in the same ApoE knockout model, silencing activity was found to be restored by the use of an exogenous GalNAc ligand that targets the iLNP to the asialoglycoprotein receptor (ASGR) expressed on hepatocytes, with as little as 0. (thefreedictionary.com)
  • These results indicate that chronic ethanol administration decreases ligand binding due to a decreased number of receptors and impairs intracellular processing of ASOR in hepatocytes. (nih.gov)
  • The human asialoglycoprotein receptor, an endocytic transport receptor of the basolateral surface of hepatocytes, is a hetero-oligomer of two homologous subunits H1 and H2. (uzh.ch)
  • These findings have major implications for therapeutic procedures attempting to target cytotoxic agents or DNA to hepatocytes using the ASGP-R. In addition, these findings demonstrate that late-stage spermatids are transcriptionally active and suggest that the RHL-1 receptor may have a functional role in sperm maturation and/or fertilization. (aspetjournals.org)
  • Gene transfer into hepatocytes using asialoglycoprotein receptor mediated endocytosis of DNA complexed with an artificial tetra-antennary galactose ligand. (semanticscholar.org)
  • The asialoglycoprotein-receptor (ASGP-R) is a C-type lectin predominantly expressed on the sinusoidal surface of mammalian hepatocytes and is responsible for the blood-clearance of desialylated glycoproteins by receptormediated endocytosis. (unibas.ch)
  • The presence of this specific receptor in high numbers on human hepatocytes attracts scientists allover the world to evaluate its potential as a mediator for the targeting of therapeutic agents and foreign genes to the liver. (unibas.ch)
  • Is there a difference in specificity of ASGP-receptors on hepatocytes, peritoneal blood macrophages and Kupffercells? (unibas.ch)
  • The asialoglycoprotein (ASGP) receptor undergoes constitutive endocytosis through the coated pit/coated vesicle pathway in hepatocytes. (elsevier.com)
  • The overall working hypothesis of this study is that ASGP receptors establish connections between hepatocytes and activated T lymphocytes (immune cells that are known to accumulate following liver injury) which facilitate the beneficial removal of potentially damaging T cells. (grantome.com)
  • In this study, we investigated the background of hepatocyte proliferation for the springboard of control between proliferation and differentiation of hepatocytes, and we focused attention to the asialoglycoprotein receptors (ASGP-R) of the hepatocytes. (elsevier.com)
  • Recent studies suggest that the H1 subunit of the carbohydrate recognition domain (H1CRD) of the asialoglycoprotein receptor is used as an entry site into hepatocytes by hepatitis A and B viruses and Marburg virus. (unibas.ch)
  • On the sinusoidal surface of the hepatocytes, asialoglycoprotein receptors are expressed abundantly which removes the asialo (galactose-terminal) glycoproteins from the sinusoidal circulation by internalizing the bound protein via endocytosis. (thefreedictionary.com)
  • The number of Asialoglycoprotein (ASGP) receptors on the hepatocytes of patients with liver disease is reduced and is thus considered a good indicator for the evaluation of liver function. (ihep.ac.cn)
  • Asialoglycoprotein Receptor Function of Hepatocytes in Patients with Autoimmune Hepatitis. (nii.ac.jp)
  • Function of Asialoglycoprotein Receptor of Hepatocytes in Patients with Wilson's Disease. (nii.ac.jp)
  • Here, we report the development and validation of a unique magnetic cell separation system, which allows enumeration, immunomorphologic identification, and genetic analysis of CTCs from peripheral blood samples of HCC patients, mediated by the interaction of the asialoglycoprotein receptor exclusively expressed on hepatocytes with its ligand. (aacrjournals.org)
  • The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. (acris-antibodies.com)
  • Sendai virosomes, derived from Sendai viral envelopes, home to hepatocytes based on the liver specific expression of asialoglycoprotein receptors (ASGPRs) which are recognized by the Sendai virosomal fusion (F) proteins. (biomedcentral.com)
  • Sendai virosomes specifically fuse with the hepatocytes through their fusion (F) protein's terminal galactose moiety, that binds specifically with asialoglycoprotein receptors (ASGPRs) present only on the surface of hepatocytes [ 14 ]. (biomedcentral.com)
  • A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. (curehunter.com)
  • When preparations of rat liver receptor for asialoglycoproteins (rat hepatic lectin, (RHL] are examined by dodecyl sulfate-polyacrylamide gel electrophoresis, multiple polypeptide species are found to be present. (semanticscholar.org)
  • The purpose of this study was to define the distribution of the asialoglycoprotein receptor (ASGP-R) main peptide, rat hepatic lectin (RHL)-1, within the rat liver lobule and to investigate its possible modulation in physiological states characterised by marked changes of receptorial expression. (univaq.it)
  • Both Ebola and Marburg viruses use multiple entry mechanisms via receptors such as: hepatocyte receptor asialoglycoprotein , folate receptor [alpha], dendritic-cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), DC-SIGN-related factors, [beta]1 integrin receptors and human macrophage galactose- and N-acetylgalactosamine-specific C type lectin. (thefreedictionary.com)
  • Type I IFN production is modulated by several receptors, including the type II C-type lectin domain family 4, member C (CLEC4C). (pubmedcentralcanada.ca)
  • In vivo targeting of C-type lectin receptors is an effective strategy for increasing antigen uptake and presentation by dendritic cells (DCs). (bloodjournal.org)
  • DCs recognize pathogens through various pattern-recognition receptors, including toll-like receptors and C-type lectin receptors (CLRs). (bloodjournal.org)
  • The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. (acris-antibodies.com)
  • Feinberg H, Taylor ME, Weis WI (2007) Scavenger receptor C-type lectin binds to the leukocyte cell surface glycan Lewis x by a novel mechanism. (functionalglycomics.org)
  • Coombs PJ, Graham SA, Drickamer K, Taylor ME (2005) Selective binding of the scavenger receptor C-type lectin to Lewisx trisaccharide and related glycan ligands. (functionalglycomics.org)
  • Other C-type lectins on macrophages include the mannose receptor, mincle [13] , macrophage C- type lectin (MCL), and dectin-1. (functionalglycomics.org)
  • Isolation and characterization of a receptor lectin specific for galactose/N-acetylgalactosamine from macrophages. (functionalglycomics.org)
  • Clone REA608 recognizes the human and rat asialoglycoprotein receptor 1 (ASGPR1) antigen, a single-pass type II membrane protein, also known as C-type lectin domain family 4 member H1 (CLEC4H1) or hepatic lectin H1 (HL-1). (miltenyibiotec.com)
  • Background: This gene encodes a subunit of the asialoglycoprotein receptor. (antikoerper-online.de)
  • The major subunit of the human asialoglycoprotein receptor contains signals for efficient endocytosis and specific basolateral expression in polarized Madin-Darby canine kidney cells, both of which are located within its 40-residue cytoplasmic domain. (uzh.ch)
  • Here, we have investigated sorting determinants within subunit H2 and their contribution to the targeting of the hetero-oligomeric receptor complex. (uzh.ch)
  • In cells expressing H1(5A) together with H2, however, subunit H2 directed receptor complexes exclusively to the basolateral domain. (uzh.ch)
  • Thus, whereas the endocytosis signal of the hetero-oligomeric asialoglycoprotein receptor resides exclusively in subunit H1, polarized transport to the basolateral domain of Madin-Darby canine kidney cells may involve two signals, only one of which is active for endocytosis. (uzh.ch)
  • Thyroglobulin binding and TSH regulation of the RHL-1 subunit of the asialoglycoprotein receptor in rat thyroid. (semanticscholar.org)
  • This gene encodes a subunit of the asialoglycoprotein receptor. (mybiosource.com)
  • Asialoglycoprotein receptor deficiency in mice lacking the minor receptor subunit. (jax.org)
  • The GP1 subunit is required for receptor interactions, and the transmembrane-associated GP2 subunit is required for membrane fusion. (asm.org)
  • They are composed of a catalytically active A-subunit and a receptor binding B-subunit. (rupress.org)
  • The B-subunit binds with high affinity to the cellular toxin receptor, the glycolipid globotriaosylceramid ( Lingwood, 1993 ). (rupress.org)
  • Synthetic peptides corresponding to ASGR2 (asialoglycoprotein receptor 2) The peptide sequence was selected from the N terminal of ASGR2. (novusbio.com)
  • These Asgr2 knock-out mice exhibit reduced expression of asialoglycoprotein receptor 1 (ASGR1). (jax.org)
  • Using the hepatocyte-specific asialoglycoprotein receptor and the substance that specifically binds to it (i. (thefreedictionary.com)
  • In preliminary studies for this proposal, we have demonstrated that the ASGP receptor specifically binds to lymphocytes and that the absence of the hepatic receptor results in the accumulation of intrahepatic T cells and enhanced liver injury. (grantome.com)
  • a mAb binds to a target (e.g., receptors, soluble antigens, etc.) and induces either antagonistic (i.e., blocking or neutralizing) or agonistic effects (i.e., activating), triggering down-stream pharmacological effects, leading to efficacy and/or unwanted side effects. (springer.com)
  • 99m Tc-DTPA-galactosyl human serum albumin ( 99m Tc-GSA) is a newly developed liver imaging ligand which specifically binds to asialoglycoprotein receptor on the hepatic cell surface. (fujita-hu.ac.jp)
  • The mannose receptor binds sugars such as mannose, N -acetylglucosamine, and fucose that are not common in terminal positions on mammalian oligosaccharides but are frequently found on the surfaces of microorganisms. (jimmunol.org)
  • Radiation inactivation and sedimentation equilibrium analysis were used to determine the functional and physical size of the chicken hepatic membrane receptor that binds N-acetylglucosamine-terminated glycoproteins. (nih.gov)
  • The modified virus or cell binds the receptor in vive and is internalized by the target cell. (google.es)
  • Our patient had tested negative for acute and chronic viral hepatitis as well as asialoglycoprotein receptor antibodies. (thefreedictionary.com)
  • On cellular level, methods for visualization of binding and internalization of synthetic drug carriers and antibodies raised against the receptor and the study of their receptor specificity (hepatic ASGP receptor versus galactosespecific receptors on the surfaces of other cells) were established. (unibas.ch)
  • Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor antibody. (elsevier.com)
  • Western Blot: Asialoglycoprotein Receptor 2 Antibody [NBP1-52958] - Fetal Lung tissue at a concentration of 0.5ug/ml. (novusbio.com)
  • Immunohistochemistry-Paraffin: Asialoglycoprotein Receptor 2 Antibody [NBP1-52958] - Human Intestine Tissue, antibody concentration 4-8ug/ml. (novusbio.com)
  • Immunohistochemistry: Asialoglycoprotein Receptor 2 Antibody [NBP1-52958] - Human cilia Cellular data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X. (novusbio.com)
  • Ethanol administration for 5-7 weeks decreased intracellular ASOR receptor content by 40%, a result which was confirmed by using both a ligand-binding assay and an antibody-binding assay. (nih.gov)
  • Although there is much interest in the use of the ASGP-R for hepatotropic drug delivery, no attempt has been made to address the receptor using an antibody instead of the usual sugar-based delivery systems. (unibas.ch)
  • In order to develop a biopharmaceutical drug, phage display technology was used to select a human scFv antibody with high affinity to the H1-CRD of the ASGP-receptor that could be applied as carrier in the form of an immuno-conjugate for the hepatotropic targeting of drugs and genes (e.g.siRNA). (unibas.ch)
  • HCC cells were bound by biotinylated asialofetuin, a ligand of asialoglycoprotein receptor, and subsequently magnetically labeled by antibiotin antibody-coated magnetic beads, followed by magnetic separation. (aacrjournals.org)
  • We find that disruption of this shield through targeted mutagenesis leads to an increase in cell entry, protease sensitivity, and antiserum/antibody sensitivity but is not sufficient to allow virion binding to the intracellular receptor NPC1. (asm.org)
  • The asialoglycoprotein receptors are lectins which bind asialoglycoprotein and glycoproteins from which a sialic acid has been removed to expose galactose residues. (wikipedia.org)
  • Examination of the pharmacokinetics of FST-ΔHBS-Fc molecules containing variable sialic acid content in asialoglycoprotein receptor 1 (ASPGR-1) knockout mice supports the receptor's role as part of the clearance mechanism of the molecules. (aspetjournals.org)
  • The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). (acris-antibodies.com)
  • Modulation of mannose and asialoglycoprotein receptor expression determines glycoprotein hormone half-life at critical points in the reproductive cycle. (semanticscholar.org)
  • Macrophage mannose receptor mediates first-line defense against pathogens by direct phagocytosis ( 2 ). (jimmunol.org)
  • The mannose receptor represents a paradigm for the involvement of C-type lectins in clearance of circulating glycoproteins. (functionalglycomics.org)
  • While the asialoglycoprotein receptor would be considered the founder member of this group of receptors, the in vivo evidence for its function is less compelling than the results for the mannose receptor, which has well defined roles in clearance of sulfated glycoprotein hormones and mannose-bearing glycoproteins released at sites of inflammation. (functionalglycomics.org)
  • As in the case of P-Selectin , much of the evidence for functions of the mannose receptor pre-dates the CFG. (functionalglycomics.org)
  • For a complete list of CFG data and resources relating to this paradigm, see the CFG database search results for "mannose receptor" . (functionalglycomics.org)
  • The CFG analyzed patterns of mannose receptor expression. (functionalglycomics.org)
  • The CFG analyzed the binding specificities of the mannose receptor. (functionalglycomics.org)
  • all glycan array results for the mannose receptor [http://www.functionalglycomics.org/glycomics/search/jsp/result.jsp?query=mannose%20AND%20receptor&cat=coreh here]. (functionalglycomics.org)
  • Synthetic peptide within Human Asialoglycoprotein Receptor 1/HL-1 aa 100-149 (internal sequence). (abcam.com)
  • The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. (antikoerper-online.de)
  • Late-stage spermatids are characterized by expression of the "liver-specific" asialoglycoprotein receptor, RHL-1. (aspetjournals.org)
  • N-lactosyl-dioleoylphosphatidylethanolamine (Lac-DOPE) was synthesized and evaluated as a liver-specific targeting ligand via asialoglycoprotein receptors for liposomal delivery of doxorubicin. (dovepress.com)
  • The receptors, which are located on liver cells, remove the target glycoproteins from circulation. (wikipedia.org)
  • It transports these glycoproteins via a series of membrane vesicles and tubules to an acidic-sorting organelle where the receptor and ligand dissociates. (novusbio.com)
  • This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. (antikoerper-online.de)
  • Chemical modification of cells to alter their surface proteins markedly reduced their susceptibility to VSVΔG*-ResGP but not to VSVΔG*-G. These findings suggest that cell surface glycoproteins with N-linked oligosaccharide chains contribute to the entry of Ebola viruses, presumably acting as a specific receptor and/or cofactor for virus entry. (pnas.org)
  • Characterization of the ASGP receptor revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). (acris-antibodies.com)
  • The receptor recognizes terminal galactose and N-acetylgalactosamine units. (abcam.com)
  • The human receptor consists of two homologous subunits, H1 and H2, whose carbohydrate recognition domains (CRDs) specifically bind non-reducing galactose- and N-acetylgalactosamine-residues. (unibas.ch)
  • Within the scope of this thesis a new cell-free competitive binding assay was developed to confirm the basic approach of new rationally designed ligands and to compare their affinities to the receptor with known galactose-derived compounds. (unibas.ch)
  • The galactose group is recognizable to the hepatic asialoglycoprotein receptors. (thefreedictionary.com)
  • Coombs PJ, Taylor ME, Drickamer K (2006) Two categories of mammalian galactose-binding receptors distinguished by glycan array profiling. (functionalglycomics.org)
  • Consistent with this observation, ligand uptake by receptors composed of H1 lacking tyrosine 5 and H2 was inefficient. (uzh.ch)
  • In the presence of 20 mM lactose, the uptake of Lac-L-calcein was inhibited, suggesting that asialoglycoprotein receptors mediated the observed cellular uptake. (dovepress.com)
  • The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero-oligomers between these major and minor forms The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. (acris-antibodies.com)
  • The role of glycan-binding receptors as tags for uptake and turnover was one of the first established functions for endogenous sugar-binding proteins and provides a key model for how glycans can modulate communication between cells in a physiological context. (functionalglycomics.org)
  • The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. (antikoerper-online.de)
  • The kinetics of appearance of newly synthesized wild-type and mutant receptor protein at the apical and basolateral surfaces indicate that these proteins are sorted intracellularly and are transported directly to the respective domains. (uzh.ch)
  • Studies on HepG2 cells have shown that the receptor is phosphorylated at serine under control conditions and following protein kinase C stimulation. (elsevier.com)
  • Macrophages express several types of C-type lectins, such as macrophage mannnose receptor and macrophage asialoglycoprotein binding protein (M-ASGP-BP). (jimmunol.org)
  • In the present study, we generated a recombinant VSV that contains the green fluorescent protein (GFP) gene, instead of the G protein gene, and thus is not infectious unless the envelope protein responsible for receptor binding and membrane fusion is provided in trans. (pnas.org)
  • The transcriptional regulators HNF1α (a homeodomain protein), HNF4α (a nuclear receptor), and HNF6 (a member of the onecut family) operate cooperatively in a connected network in the liver, but less is known about the structure of this regulatory network in human pancreatic islets. (sciencemag.org)
  • 4. The method of claim 1 wherein the antithrombotic liposomes of step (d) comprise an antithrombotic protein selected from the group consisting of thrombomodulin, a truncated thrombomodulin, endogenous protein C, an endothelial protein C receptor, and an endogenous protein C activator protein. (google.com)
  • 3 , 4 FVIII clearance from circulation has been partly attributed to specific binding to the low-density lipoprotein receptor-related protein (LRP1), a hepatic clearance receptor with broad ligand specificity. (bloodjournal.org)
  • Recent work demonstrated that the Niemann-Pick C1 (NPC1) protein is an essential entry receptor for filoviruses. (asm.org)
  • B-fragment sorting at the level of early/recycling endosomes seemed to involve vesicular coats, since brefeldin A treatment led to B-fragment accumulation in transferrin receptor-containing membrane tubules, and since B-fragment colocalized with adaptor protein type 1 clathrin coat components on early/recycling endosomes. (rupress.org)
  • Effect of chronic ethanol administration on total asialoglycoprotein receptor content and intracellular processing of asialoorosomucoid in isolated. (nih.gov)
  • In this study, we further characterized these impairments by examining the content of intracellular receptors and by investigating ligand processing directed by these intracellular receptors. (nih.gov)
  • In addition to a decreased number of intracellular receptors, an impairment in intracellular processing of receptor-ligand complexes was identified. (nih.gov)
  • In ethanol-fed animals, dissociation of receptor-ligand complexes was decreased during steady-state conditions of endocytosis at 37 degrees C. Impaired receptor-ligand dissociation did not alter the fate of the ligand which was to undergo diacytosis (ligand recycling), but did appear to impair degradation of intracellular ligand. (nih.gov)
  • Nevertheless, it was not until 1984 when the first Cyp, specifically cyclophilin A (Cyp A), was purified from bovine thymocytes, and it was confirmed as the primary intracellular receptor of the immunosuppressive drug CsA ( 2 ). (frontiersin.org)
  • In particular, our laboratory identified multiple ethanol-induced alterations in the process of receptor- mediated endocytosis (RME) and more specifically, we discovered that ethanol treatment resulted in marked impairments in the function of the hepatocyte-specific asialoglycoprotein (ASGP) receptor. (grantome.com)
  • The isolated receptor is a hetero-oligomeric complex constituted of two subunits with molecular masses of 40- and 47-kDa. (mun.ca)
  • Can monovalent high affinity ligands be designed which specifically bind to the hepatic receptor. (unibas.ch)
  • We show that an electroactive interface confined with densely clustered galactosyl ligands is able to ingeniously recognize the asialoglycoprotein receptors on live Hep-G2 cells employing simple electrochemical techniques. (nature.com)
  • Autoantibodies detected in CLDs target a wide repertoire of host antigens, which include proteins from the nucleus, the cytoskeleton, liver kidney microsomes, the mitochondria, and the cytoplasm of neutrophilic granulocytes, as well as liver soluble antigens, asialoglycoprotein receptors, TAA, and stress proteins [21]. (thefreedictionary.com)
  • Because filoviruses contain a single transmembrane GP that forms spikes on the virion surface, the GP is most likely to be responsible for receptor binding and membrane fusion, leading to virus penetration. (pnas.org)
  • We present here the simple construction of a unique class of biomimetic interfaces based on a graphene-mediated self-assembly of glycosyl anthraquinones to a screen-printed electrode for the detection of transmembrane glycoprotein receptors expressed on a hepatoma cell line. (nature.com)
  • Activation of type I IFN secretion depends on the pattern recognition receptors TLR7 and TLR9, which sense microbial RNA and DNA, respectively. (pubmedcentralcanada.ca)
  • Here, we review the pattern recognition receptors that identify invading microbes, as well as the innate immune effector mechanisms that they activate in zebrafish embryos. (hindawi.com)
  • The innate immune system utilizes a limited number of germline-encoded pattern recognition receptors (PRRs) to recognize evolutionary conserved structures on pathogens, named pathogen-associated molecular patterns (PAMPs) [ 15 ]. (hindawi.com)
  • In addition to creating the knockout for the two mouse forms of MGL, PIs have been involved in extensive studies of binding specificity and mechanism of ligand binding as well as the role of the receptor in macrophage signaling. (functionalglycomics.org)
  • In situ hybridization studies demonstrate that late-stage mouse and rat spermatids are the testicular cells that express the RHL-1 ASGP-R. The rat spermatid RHL-1 receptor has functional binding capability, with a KD of approximately 1.4 x 10(-8) M, and similar characteristics, compared with the hepatic RHL-1 receptor, regarding ligand binding specificity and ion dependence. (aspetjournals.org)
  • Hence, this study aimed to determine if ASGP receptor function is required to protect against liver injury by utilizing a knockout mouse model lacking functional ASGP receptor in the setting of carbon tetrachloride (CCl 4 ) hepatotoxicity. (ovid.com)
  • And finally, in vivo studies are proposed to demonstrate the importance of altered or absent functional ASGP receptors in the development of immune cell related liver injury using models of ethanol administration along with an ASGP receptor knockout mouse treated with inducers T cell-mediated hepatitis. (grantome.com)
  • Before funding for knockout mice was discontinued, the CFG developed the DNA construct to create a mouse line lacking the scavenger receptor. (functionalglycomics.org)
  • In this disease, a defect in the low density lipoprotein (LDL) receptor gene results in abnormal expression of the LDL receptor and consequent failure of clearance of LDL cholesterol. (bmj.com)
  • 5 - 7 The low-density lipoprotein receptor was also shown to play a role in FVIII clearance, potentially by cooperating with LRP1. (bloodjournal.org)
  • The asialoglycoprotein receptor: a model for endocytic transport receptors. (semanticscholar.org)
  • Therefore, MGL acts as an efficient endocytic antigen receptor on dermal DCs in vivo, able to prime Tn-specific T- and B-cell responses. (bloodjournal.org)
  • As such, our results implied that GAL-AN and GAL-DOX-AN have specific interaction with HepG2 cells via the recognition of GAL and asialoglycoprotein receptor, which renders GAL-AN a promising anticancer drug delivery carrier for liver cancer therapy. (curehunter.com)
  • Quan GL, Pan X, Wang ZH, Wu QL, Li G, Dian LH et al (2015) Lactosaminated mesoporous silica nanoparticles for asialoglycoprotein receptor targeted anticancer drug delivery. (biomedcentral.com)
  • Evaluation of GalNAc-siRNA Conjugate Activity in Pre-clinical Animal Models with Reduced Asialoglycoprotein Receptor Expression. (semanticscholar.org)
  • Receptor-mediated gene delivery and expression in vivo. (semanticscholar.org)
  • Increased expression of c-met, a potent HGF receptor, preceded the surge of DNA synthesis in the process of liver cell reganeration. (nii.ac.jp)
  • In the present paper, we studied the effect of these four compounds on the surface membrane CD147 receptor expression, on the extracellular levels of Cyp A and on the ability to migrate of concanavalin (Con A)-activated T lymphocytes. (frontiersin.org)
  • Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. (acris-antibodies.com)
  • The two subunits of the asialoglycoprotein receptor contain different sorting information. (uzh.ch)
  • Coexpression of both subunits yielded ligand-binding receptors located specifically on the basolateral surface. (uzh.ch)
  • Differential ligand binding by two subunits of the rat liver asialoglycoprotein receptor. (semanticscholar.org)
  • e.g., what are the specific functions of the receptor subunits H1 and H2? (unibas.ch)
  • Interestingly, exposing the receptor-binding domain (RBD) by removing the glycan shield did not allow interaction with the endosomal receptor, NPC1, indicating that the glycan cap/MLD domains mask RBD residues required for binding. (asm.org)
  • We will address these hypotheses with the following specific aims: In initial studies, the recognition and binding of T lymphocytes to the hepatocyte ASGP receptor will be characterized in vitro. (grantome.com)
  • However, none of the proposed candidate receptors has proven essential in all in vitro scenarios, nor have they proven essential when evaluated using animal models. (asm.org)
  • 11. An in vitro process for introducing foreign DNA by means of human adenovirus into human cells which have no or only a small number of adenovirus receptors or the adenovirus receptors of which are wholly or partly blocked, characterized in that the cells are brought into contact with a conjugate as described in claim 5. (google.es)
  • Lactobionic acid has been used as a targeting moiety for drug delivery to cells expressing asialoglycoprotein receptors. (wikipedia.org)
  • The major and minor forms of the asialoglycoprotein receptor (ASGP-R), designated in the rat as RHL-1 and RHL-2/3, respectively, have traditionally been considered to be expressed exclusively in hepatic parenchymal cells. (aspetjournals.org)
  • ab42488 staining Asialoglycoprotein Receptor 1 (red) in rat hepatocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). (abcam.cn)
  • This study examined whether the ASGP receptor could also serve as a substrate for a tyrosine kinase in HepG2 cells. (elsevier.com)
  • 32 P labeling was performed in membrane preparations, in permeabilized cells at 4°C, and in intact cells at 37°C. The phosphorylated ASGP receptor was isolated by immunoprecipitation, hydrolyzed in 6 N HCl at 110°C, and analyzed by two-dimensional high voltage electrophoresis. (elsevier.com)
  • ASGP receptor phosphorylation at both tyrosine and serine was detected in intact cells incubated with phosphatase inhibitors for 60 min at 37°C. The presence of both phenylarsine oxide (20 μM) and sodium orthovanadate (200 μM) was required for tyrosine phosphorylation. (elsevier.com)
  • Recently it has been suggested that the recognition and subsequent regulation of immune cells could be a potential physiological role of the ASGP receptor. (grantome.com)
  • Therefore, we propose that the ASGP receptor has a role in the regulation of immune cells in the healthy liver and that ethanol-induced impairments in ASGP receptor function can result in increased liver injury caused by T cell- mediated events. (grantome.com)
  • Furthermore, as a consequence of ethanol-mediated alterations to the ASGP receptor, altered clearance of T cells occurs leading to abnormal lymphocyte accumulation, events that could contribute the development of hepatitis and the progression of ALD. (grantome.com)
  • We show that the designed system has the ability to exquisitely capture glycoprotein receptors exclusively expressed on live cancer cells employing facile electrochemical methods and economic facilities. (nature.com)
  • Liver cell adherence and ligand-receptor binding of HGF depended on the ECM where the cells sit on, including molecular species of proteoglycans. (nii.ac.jp)
  • In the present study, transfected cells were maintained in the absence or presence of 25 mM ethanol for 7 days, and alterations in endocytosis by the asialoglycoprotein receptor were determined. (hindawi.com)
  • The B cells in these mice are hyper-responsive to stimulation through the B cell receptor. (stanford.edu)
  • We are additionally studying the mechanisms by which CD72-deficiency leads to a partial abrogation of B cell anergic tolerance in mice in which all B cells express a transgenic B cell receptor specific for hen-egg lysozyme (HEL) and in which the antigen HEL is expressed in the serum. (stanford.edu)
  • The receptor then returns to the cell membrane surface. (abcam.com)
  • Use of these inhibitors together resulted in a 16.4-fold increase in phosphorylation of the immunoprecipitated ASGP receptor, whereas phosphorylation of total HepG2 membrane proteins was not significantly augmented by this procedure. (elsevier.com)
  • The sole viral envelope glycoprotein, which is a principal immunogenic target, contains a heavy shield of glycans surrounding the conserved receptor-binding domain. (asm.org)
  • Chronic ethanol administration markedly impairs the process of receptor-mediated endocytosis (RME) of a representative asialoglycoprotein, asialoorosomucoid (ASOR), by the liver (Casey et al. (nih.gov)
  • Ethanol administration has been shown to alter receptor-mediated endocytosis in the liver. (hindawi.com)
  • Our results showed that ethanol metabolism impaired internalization of asialoorosomucoid, a ligand for the asialoglycoprotein receptor. (hindawi.com)
  • Selective proteolytic digestion of ASGP receptors is isolated vesicles demonstrated that the phosphorylation site identified in these studies is located at tyrosine 5 in the cytoplasmic tail. (elsevier.com)
  • 5 The asialoglycoprotein receptor, which is abundantly expressed in the liver, could also be involved in the catabolism of FVIII, and the binding to asialoglycoprotein receptor is primarily mediated by the B domain of FVIII. (bloodjournal.org)
  • This resulted in a 30-40% reduction in serum cholesterol, with the recombinant LDL receptor being detectable for up to six months. (bmj.com)
  • 3 This was achieved by segmental hepatic resection, preparation of hepatocyte cultures, and transduction of these cultures with a recombinant retrovirus encoding the gene for the human LDL receptor. (bmj.com)