An enzyme that converts ascorbic acid to dehydroascorbic acid. EC 1.10.3.3.
A copper-containing oxidoreductase enzyme that catalyzes the oxidation of 4-benzenediol to 4-benzosemiquinone. It also has activity towards a variety of O-quinols and P-quinols. It primarily found in FUNGI and is involved in LIGNIN degradation, pigment biosynthesis and detoxification of lignin-derived products.
A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.
A genus (formerly part of Rhus genus) of shrubs, vines, or trees that yields a highly allergenic oleoresin which causes a severe contact dermatitis (DERMATITIS, TOXICODENDRON). The most toxic species are Toxicodendron vernix (poison sumac), T. diversilobum (poison oak), and T. radicans (poison ivy). T. vernicifera yields a useful varnish from which certain enzymes (laccases) are obtained.
Use of a pulse of X-rays or fast electrons to generate free radicals for spectroscopic examination.
A mitosporic fungal genus with many reported ascomycetous teleomorphs. Cephalosporin antibiotics are derived from this genus.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.
A creeping annual plant species of the CUCURBITACEAE family. It has a rough succulent, trailing stem and hairy leaves with three to five pointed lobes.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Peroxidases that utilize ASCORBIC ACID as an electron donor to reduce HYDROGEN PEROXIDE to WATER. The reaction results in the production of monodehydroascorbic acid and DEHYDROASCORBIC ACID.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
A flavoprotein enzyme that catalyzes the univalent reduction of OXYGEN using NADPH as an electron donor to create SUPEROXIDE ANION. The enzyme is dependent on a variety of CYTOCHROMES. Defects in the production of superoxide ions by enzymes such as NADPH oxidase result in GRANULOMATOUS DISEASE, CHRONIC.
A bacterial protein from Pseudomonas, Bordetella, or Alcaligenes which operates as an electron transfer unit associated with the cytochrome chain. The protein has a molecular weight of approximately 16,000, contains a single copper atom, is intensively blue, and has a fluorescence emission band centered at 308nm.
A plant genus of the family CUCURBITACEAE, order Violales, subclass Dilleniidae, which includes pumpkin, gourd and squash.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
Exclusive legal rights or privileges applied to inventions, plants, etc.
An early local inflammatory reaction to insult or injury that consists of fever, an increase in inflammatory humoral factors, and an increased synthesis by hepatocytes of a number of proteins or glycoproteins usually found in the plasma.
Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).
Rare cutaneous eruption characterized by extensive KERATINOCYTE apoptosis resulting in skin detachment with mucosal involvement. It is often provoked by the use of drugs (e.g., antibiotics and anticonvulsants) or associated with PNEUMONIA, MYCOPLASMA. It is considered a continuum of Toxic Epidermal Necrolysis.
Fluids restored to the body in order to maintain normal water-electrolyte balance.
Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use.
Therapy whose basic objective is to restore the volume and composition of the body fluids to normal with respect to WATER-ELECTROLYTE BALANCE. Fluids may be administered intravenously, orally, by intermittent gavage, or by HYPODERMOCLYSIS.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The condition that results from excessive loss of water from a living organism.
Usually inert substances added to a prescription in order to provide suitable consistency to the dosage form. These include binders, matrix, base or diluent in pills, tablets, creams, salves, etc.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
Solid dosage forms, of varying weight, size, and shape, which may be molded or compressed, and which contain a medicinal substance in pure or diluted form. (Dorland, 28th ed)
Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.
Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
The ability of a protein to retain its structural conformation or its activity when subjected to physical or chemical manipulations.
A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.
A plant species of the family APIACEAE that is widely cultivated for the edible yellow-orange root. The plant has finely divided leaves and flat clusters of small white flowers.
A plant species of the family CUCURBITACEAE, order Violales, subclass Dilleniidae known for the melon fruits with reticulated (net) surface including cantaloupes, honeydew, casaba, and Persian melons.
Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)
An organism of the vegetable kingdom suitable by nature for use as a food, especially by human beings. Not all parts of any given plant are edible but all parts of edible plants have been known to figure as raw or cooked food: leaves, roots, tubers, stems, seeds, buds, fruits, and flowers. The most commonly edible parts of plants are FRUIT, usually sweet, fleshy, and succulent. Most edible plants are commonly cultivated for their nutritional value and are referred to as VEGETABLES.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
One of the protein CROSS-LINKING REAGENTS that is used as a disinfectant for sterilization of heat-sensitive equipment and as a laboratory reagent, especially as a fixative.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
Substances that are used in place of blood, for example, as an alternative to BLOOD TRANSFUSIONS after blood loss to restore BLOOD VOLUME and oxygen-carrying capacity to the blood circulation, or to perfuse isolated organs.
The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements.
Adaptation to a new environment or to a change in the old.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.

Azide binding to the trinuclear copper center in laccase and ascorbate oxidase. (1/79)

Azide binding to the blue copper oxidases laccase and ascorbate oxidase (AO) was investigated by electron paramagnetic resonance (EPR) and pulsed electron-nuclear double resonance (ENDOR) spectroscopies. As the laccase : azide molar ratio decreases from 1:1 to 1:7, the intensity of the type 2 (T2) Cu(II) EPR signal decreases and a signal at g approximately 1.9 appears. Temperature and microwave power dependent EPR measurements showed that this signal has a relatively short relaxation time and is therefore observed only below 40 K. A g approximately 1.97 signal, with similar saturation characteristics was found in the AO : azide (1:7) sample. The g < 2 signals in both proteins are assigned to an S = 1 dipolar coupled Cu(II) pair whereby the azide binding disrupts the anti-ferromagnetic coupling of the type 3 (T3) Cu(II) pair. Analysis of the position of the g < 2 signals suggests that the distance between the dipolar coupled Cu(II) pair is shorter in laccase than in AO. The proximity of T2 Cu(II) to the S = 1 Cu(II) pair enhances its relaxation rate, reducing its signal intensity relative to that of native protein. The disruption of the T3 anti-ferromagnetic coupling occurs only in part of the protein molecules, and in the remaining part a different azide binding mode is observed. The 130 K EPR spectra of AO and laccase with azide (1:7) exhibit, in addition to an unperturbed T2 Cu(II) signal, new features in the g parallel region that are attributed to a perturbed T2 in protein molecules where the anti-ferromagnetic coupling of T3 has not been disrupted. While these features are also apparent in the AO : azide sample at 10 K, they are absent in the EPR spectra of the laccase : azide sample measured in the range of 6-90 K. Moreover, pulsed ENDOR measurements carried out at 4.2 K on the latter exhibited only a reduction in the intensity of the 20 MHz peak of the 14N histidine coordinated to the T2 Cu(II) but did not resolve any significant changes that could indicate azide binding to this ion. The lack of T2 Cu(II) signal perturbation below 90 K in laccase may be due to temperature dependence of the coupling within the trinuclear : azide complex.  (+info)

Homology modeling of the multicopper oxidase Fet3 gives new insights in the mechanism of iron transport in yeast. (2/79)

Fet3, the multicopper oxidase of yeast, oxidizes extracellular ferrous iron which is then transported into the cell through the permease Ftr1. A three-dimensional model structure of Fet3 has been derived by homology modeling. Fet3 consists of three cupredoxin domains joined by a trinuclear copper cluster which is connected to the blue copper site located in the third domain. Close to this site, which is the primary electron acceptor from the substrate, residues for a potential iron binding site could be identified. The surface disposition of negatively charged residues suggests that Fet3 can translocate Fe(3+) to the permease Ftr1 through a pathway under electrostatic guidance.  (+info)

Effects of superoxide anion generators and thiol modulators on nitrergic transmission and relaxation to exogenous nitric oxide in the sheep urethra. (3/79)

The effects of superoxide anion generators, the nitric oxide (NO) scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoine-1-oxyl 3-oxide (carboxy-PTIO), the specific guanylate cyclase inhibitor 1H-[1,2,4]-oxadiazole-[4,3-a]-quinoxalin-1-one (ODQ), and thiol modulating agents were investigated on relaxations induced by nitrergic stimulation and exogenous NO addition in the sheep urethra. Methylene blue (MB, 10 microM), pyrogallol (0.1 mM) and xanthine (X, 0.1 mM)/xanthine oxidase (XO, 0.1 u ml(-1)) inhibited NO-mediated relaxations, without affecting those induced by nitrergic stimulation. This resistance was not diminished following inhibition of endogenous Cu/Zn superoxide dismutase (Cu/Zn SOD) with diethyldithiocarbamic acid (DETCA, 3 mM), which almost abolished tissue SOD activity. Carboxy-PTIO (0.1 - 0.5 mM) inhibited NO-mediated relaxations but had no effect on responses to nitrergic stimulation, which were not changed by treatment with ascorbate oxidase (2 u ml(-1)). Relaxations to NO were reduced, but not abolished, by ODQ (10 microM), while nitrergic responses were completely blocked. The thiol modulators, ethacrynic acid (0.1 mM), diamide (1.5 mM), or 5,5'-dithio-bis (2-nitrobenzoic acid) (DTNB, 0. 5 mM), and subsequent treatment with dithiothreitol (DTT, 2 mM) had no effect on responses to nitrergic stimulation or NO. In contrast, N-ethylmaleimide (NEM, 0.2 mM) markedly inhibited both relaxations. L-cysteine (L-cys, 0.1 mM) had no effect on responses to NO, while it inhibited those to nitrergic stimulation, in a Cu/Zn SOD-independent manner. Our results do not support the view that the urethral nitrergic transmitter is free NO, and the possibility that another compound is acting as mediator still remains open. British Journal of Pharmacology (2000) 129, 53 - 62  (+info)

Auxin metabolism in the root apical meristem. (4/79)

Within the root meristem of flowering plants is a group of mitotically inactive cells designated the quiescent center (QC). Recent work links the quiescent state to high levels of the growth regulator auxin that accumulates in the QC via polar transport. This in turn results in elevated levels of the enzyme ascorbic acid oxidase (AAO), resulting in a reduction of ascorbic acid (AA) within the QC and mitotic quiescence. We present evidence for additional interactions between auxin, AAO, and AA, and report that, in vitro, AAO oxidatively decarboxylates auxin, suggesting a mechanism for regulating auxin levels within the QC. We also report that oxidative decarboxylation occurs at the root tip and that an intact root cap must be present for this metabolic event to occur. Finally, we consider how interaction between auxin and AAO may influence root development by regulating the formation of the QC.  (+info)

Erythrocytes reduce extracellular ascorbate free radicals using intracellular ascorbate as an electron donor. (5/79)

Ascorbate is readily oxidized in aqueous solution by ascorbate oxidase. Ascorbate radicals are formed, which disproportionate to ascorbate and dehydroascorbic acid. Addition of erythrocytes with increasing intracellular ascorbate concentrations decreased the oxidation of ascorbate in a concentration-dependent manner. Concurrently, it was found, utilizing electron spin resonance spectroscopy, that extracellular ascorbate radical levels were decreased. Control experiments showed that these results could not be explained by leakage of ascorbate from the cells, inactivation of ascorbate oxidase, or oxygen depletion. Thus, this means that intracellular ascorbate is directly responsible for the decreased oxidation of extracellular ascorbate. Exposure of ascorbate-loaded erythrocytes to higher levels of extracellular ascorbate radicals resulted in the detection of intracellular ascorbate radicals. Moreover, efflux of dehydroascorbic acid was observed under these conditions. These data confirm the view that intracellular ascorbate donates electrons to extracellular ascorbate free radical via a plasma membrane redox system. Such a redox system enables the cells to effectively counteract oxidative processes and thereby prevent depletion of extracellular ascorbate.  (+info)

Analysis of expressed sequence tags of flower buds in Lotus japonicus. (6/79)

In order to study gene expression in a reproductive organ, we constructed a cDNA library of mature flower buds in Lotus japonicus, and characterized expressed sequence tags (ESTs) of 842 clones randomly selected. The EST sequences were clustered into 718 non-redundant groups. From BLAST and FASTA search analyses of both protein and DNA databases, 58.5% of the EST groups showed significant sequence similarities to known genes. Several genes encoding these EST clones were identified as pollen-specific genes, such as pectin methylesterase, ascorbate oxidase, and polygalacturonase, and as homologous genes involved in pollen-pistil interaction. Comparison of these EST sequences with those derived from the whole plant of L. japonicus, revealed that 64.8% of EST sequences from the flower buds were not found in EST sequences of the whole plant. Taken together, the EST data from flower buds generated in this study is useful in dissecting gene expression in floral organ of L. japonicus.  (+info)

Behavioral activation in rats requires endogenous ascorbate release in striatum. (7/79)

Ascorbate (vitamin C) is found in high concentrations in the striatum in which it may play a role in behavioral activation. To test this hypothesis, freely behaving rats received bilateral intrastriatal infusions of ascorbate oxidase (AAO) to inactivate extracellular ascorbate. Slow-scan voltammetry was used simultaneously to assess changes in ascorbate and 3,4-dihydroxyphenylacetic acid (DOPAC), a major dopamine metabolite, near the infusion site. Intrastriatal AAO, but not saline vehicle, caused a rapid decline in both ascorbate and behavioral activation. Within 20 min, an ascorbate loss of 50-70% led to a near-total inhibition of all recorded behavior, including open-field locomotion, approach of novel objects, and social interactions with other rats. DOPAC levels remained stable, arguing against an AAO-induced disruption of dopamine transmission. Consistent with this interpretation, subsequent injection of 1.0 mg/kg d-amphetamine, an indirect dopamine agonist, quickly restored behavioral activation, which also was accompanied by a marked rise in extracellular ascorbate. Bilateral AAO infusions into dorsal hippocampus, which also has a high level of extracellular ascorbate, failed to alter behavioral activation, indicating that a loss of brain ascorbate per se does not suppress behavior. Collectively, these results implicate ascorbate in the behavioral operations of the striatum and suggest that the extracellular level of this vitamin plays a critical role in behavioral activation.  (+info)

Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat. (8/79)

The Bacillus subtilis endospore coat protein CotA shows laccase activity. By using comparative modeling techniques, we were able to derive a model for CotA based on the known x-ray structures of zucchini ascorbate oxidase and Cuprinus cereneus laccase. This model of CotA contains all the structural features of a laccase, including the reactive surface-exposed copper center (T1) and two buried copper centers (T2 and T3). Single amino acid substitutions in the CotA T1 copper center (H497A, or M502L) did not prevent assembly of the mutant proteins into the coat and did not alter the pattern of extractable coat polypeptides. However, in contrast to a wild type strain, both mutants produced unpigmented colonies and spores unable to oxidize syringaldazine (SGZ) and 2'2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The CotA protein was purified to homogeneity from an overproducing Escherichia coli strain. The purified CotA shows an absorbance and a EPR spectra typical of blue multicopper oxidases. Optimal enzymatic activity was found at < or =pH 3.0 and at pH 7.0 for ABTS or SGZ oxidation, respectively. The apparent K(m) values for ABTS and SGZ at 37 degrees C were of 106 +/- 11 and 26 +/- 2 microm, respectively, with corresponding k(cat) values of 16.8 +/- 0.8 and 3.7 +/- 0.1 s(-1). Maximal enzyme activity was observed at 75 degrees C with ABTS as substrate. Remarkably, the coat-associated or the purified enzyme showed a half-life of inactivation at 80 degrees C of about 4 and 2 h, respectively, indicating that CotA is intrinsically highly thermostable.  (+info)

Ascorbate oxidase contains two paramagnetic Cu(ii) binding sites, type 1 (T1) and type 2 (T2) and in both sites the Cu(ii) is coordinated to histidine residues. We use several pulse EPR techniques at high field (95 GHz) to determine ligand 1H and 14N hyperfine couplings in the two sites and identify the T1 s
Pretreatment of a serum or plasma sample with ascorbate oxidase removed interfering ascorbate and allowed the determination of cholesterol to be carried out by a current enzymatic cholesterol method available in kit form. The Cobas-Fara was programmed to carry out pretreatment of the sample with ascorbate oxidase before addition of the cholesterol colour reagent.. ...
1ASQ: X-ray structures and mechanistic implications of three functional derivatives of ascorbate oxidase from zucchini. Reduced, peroxide and azide forms.
TY - JOUR. T1 - Sensitive colorimetric assay of serum diamine oxidase. AU - Takagi, Kenji. AU - Nakao, Makoto. AU - Ogura, Yozo. AU - Nabeshima, Toshitaka. AU - Kunii, Akira. PY - 1994/4. Y1 - 1994/4. N2 - A simple and sensitive colorimetric assay for serum diamine oxidase (DAO) activity was based on a coupled reaction with peroxidase and a new chromogen, 10-(carboxymethyl-aminocarbonyl)-3,7-bis(dimethylamino) phenothiazine sodium salt (DA-67). In the presence of peroxide and DA-67, peroxidase catalyzes the formation of methylene blue having an absorption maximum at 668 nm. The proposed method eliminates the interferences occurring in serum with use of ascorbate oxidase and stops the reaction with sodium diethyldithiocarbamate, leaving the methylene blue in the reaction mixture stable for about 2 h. Low normal basal values of serum DAO can be determined in the range 2.8-9.0 units/l. Since all reagents are commercially available the method is suitable for the clinical laboratory.. AB - A simple ...
Since, it is estimated that 6000-8000 polyphenols exist in nature, a fraction have been isolated and studied, so our knowledge of these compounds, and how they interact with other antioxidants, such as those included in the antioxidant network that we discussed previously, is limited. Since all Polyphenol substances are subject to metabolic changes it is difficult to ascertain how these compounds affect our health specifically (Is it the polyphenols or the by product after metabolism that provides the benefit?). We know that Flavonoids alter Vitamin C transport and the function of Ascorbate oxidase,an enzyme that converts Vitamin C to a non vitamin form, but we dont know why, and if this relationship somehow enhances antioxidant function. It was always thought that polyphenols and other antioxidants simply scavenge free radicals as a form of protection against oxidative damage, but it is more the case of interaction of the polyphenolic compounds and their modification of redox status of the ...
Ascorbate recycling in human neutrophils: Induction by bacteria: Ascorbate (vitamin C) recycling occurs when extracellular ascorbate is oxidized, transported as
A 3,3′-bis(aminoimino)BINOL ligand was newly designed and synthesized for the formation of a trinuclear Zn complex upon reaction with Zn(OAc)2. Using the harmony of the tri-zinc atoms, 1 mol% Zn3(OAc)4-3,3′-bis(aminoimino)binaphthoxide catalyzed asymmetric iodolactonization in up to 99.9% ee.
Now Vitamins - Calcium Ascorbate 8 oz Manufacturers Description: Calcium Ascorbate is buffered and will not contribute to gastric irrit...
TY - JOUR AU - Hadži-Tašković-Šukalović, Vesna AU - Vučinić, Željko AU - Vuletić, Mirjana AU - Marković, Ksenija AU - Kravić, Natalija PY - 2016 UR - http://rik.mrizp.rs/handle/123456789/618 AB - Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic ...
Mycocosm Portal version:12.104 jgi-myco-web-2.nersc.gov Release Date:30-Jul-2020 15:17:52 PST Current Date:09-Aug-2020 23:02:43.754 PDT ...
The 2-Cys peroxiredoxin-A (2CPA) promoter is a model promoter to study redox and ABA-dependent stress signaling. Here, an Arabidopsis reporter gene line expressing luciferase under control of the 2CPA promoter was used to study the impact of ascorbate on reporter gene transcription in a series of protoplast and leaf slice incubation experiments. It was shown that ascorbate has a dual function on gene expression regulation. First, a comparison of responses to ascorbate, dehydroascorbate and reduced and oxidized glutathione demonstrated that ascorbate feeding supports gene expression regulation by increasing the catalytic capacity in redox signaling, as defined by the concentration of low molecular weight antioxidants and their oxidized counterparts. Second, ascorbate had a specific and light-dependent effect on 2CPA transcription, which cannot be substituted by reduced glutathione. Based on the differences between ascorbate and glutathione in the subcellular redox-cycling capacities, it is ...
Writers: Maja Thim Larsena, Helen Rawsthorneb etc. - Published: October 10th 2018 - Publication: Journal of Controlled Release 287 (2018) 132-141
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Viridian HIGH FIVE B-Complex and Magnesium Ascorbate is packed with nutrients to support a busy and demanding lifestyle, including all the B Complex vitamins and vitamin C to help maintain adrenal function.
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We are able to show that average z-scores for several inflammatory and angiogenesis promoting cytokines are positive, indicating that they are higher than averages for healthy controls, and that their levels decreased over the course of treatment. In addition, serum concentrations of tumor markers d …
Duconge, J. (2008). Pharmacokinetics of vitamin C: insights into the oral and intravenous administration of ascorbate. P R Health Sci J., [online] 27(1), pp.7-19 ...
Duconge, J. (2008). Pharmacokinetics of vitamin C: insights into the oral and intravenous administration of ascorbate. P R Health Sci J., [online] 27(1), pp.7-19 ...
TY - JOUR. T1 - Intramolecular Electron Transfer from Tryptophan to Guanosyl Radicals in a Linked System as a Model of DNA Repair. AU - Morozova, Olga B.. AU - Fishman, Natalya N.. AU - Yurkovskaya, Alexandra V.. PY - 2017/3/1. Y1 - 2017/3/1. N2 - As a model of chemical DNA repair, intramolecular electron transfer from tryptophan to the radical of the purine base guanosine combined into a conjugate by a flexible linker was studied by time-resolved chemically induced dynamic nuclear polarization (CIDNP). The guanosyl radicals were photochemically generated in the quenching reaction of the triplet excited dye 2,2′-dipyridyl. The CIDNP kinetics was obtained by detection of NMR spectra containing anomalously enhanced signals of diamagnetic products that are formed during a variable period after excitation by a laser pulse. The kinetic data obtained for the protons located on the guanosyl and tryptophanyl moieties of the conjugate were compared to those obtained in photoreactions of the molecules ...
zucchini nutrition details. zucchini calories, fat, protein, carbohydrates and vitamins. zucchini recipes, zucchini articles and zucchini videos.
Find help and information on Cucurbita pepo Little Gem Rolet Squash, including varieties and pruning advice. Click here to find out more.
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Cucurbita pepo, Wild hybrid.|br|Scanned with VisualSFM.|br|Wild hybrid plant without reference, not reported on the cucurbitophile.fr site.
Cucurbita pepo, is widely used like food and in folk medicine around of the world. This aims a comprehensive of the pharmacological, chemical constituents, and clinical u..
Read the Zucchini and Stomach Pain discussion from the Chowhound General Discussion, Zucchini food community. Join the discussion today.
Turn the heat to high and add the zucchini, salt, and pepper. Cook 2-3 minutes, stirring occasionally until the zucchini starts to brown. Add the chicken stock and cover. Cook 2-3 additional minutes until zucchini is soft and sprinkle with sage. Serve immediately ...
What to make w/ all your zucchini? How about these crispy Baked Parmesan Zucchini Bites by @girlmeetskitchen. All you need are 5 ingredients: Zucchini,
E. coli is able to utilize L-ascorbate (vitamin C) as the sole source of carbon under anaerobic and aerobic conditions. Ascorbic acid in the cytoplasm is processed through a spontaneous reaction with a hydrogen ion and hydrogen peroxide, producing water, dehydroascorbic acid and ascorbic acid. Dehydroascorbic acid reacts with water spontaneously producing an isomer, dehydroascorbate (bicyclic form). The compound then loses a hydrogen ion resulting in a 2,3-Diketo-L-gulonate. This compound is then reduced through a NADH dependent 2,3 diketo-L-gulonate reductase, releasing a NAD and 3-Dehydro-L-gulonate.This compound is phosphorylated through an ATP mediated L-xylulose/3-keto-L-gulonate kinase resulting in an ADP, hydrogen ion and a 3-Keto-L-gulonate 6 phosphate. L-ascorbate can also be imported and converted to L-ascorbate-6-phosphate by the L-ascorbate PTS transporter. L-ascorbate-6-phosphate reacts with a probable L-ascorbate-6-phosphate lactonase ulaG, resulting in a 3-keto-L-gulonate ...
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Sodium content and RDA percentage, per serving and per 100g, in 8 types of zucchini. The amount of Sodium is 374 mg to 2 mg per 100g, in zucchini.
Avoiding gluten doesnt mean you have to miss out on your favourite pasta dishes. Get creative by pairing strands of zucchini with a yummy pasta sauce, such as this delicious zucchini linguine with pesto.
Welcome to the famous Daves Garden website. Join our friendly community that shares tips and ideas for gardens, along with seeds and plants.
Oelrichs B.A., Kelly J.D., Kratzing C.C. and Winzor D.J. (1988) Accumulation of ascorbate in rat cerebellum. International Journal for Vitamin and Nutrition Research, 58 2: 213-217. ...
BMB Rep. 2010 Oct;43(10):688-92. Preventable effect of L-threonate, an ascorbate metabolite, on androgen-driven balding via repression of dihydrotestosterone-in
Solgar : Calcium Ascorbate Crystals - 8.8 Oz.* - Affordable Natural Supplements. Vitamins, minerals, herbals, supplements, and natural products. Free shipping on orders over $100!
Baked Hasselback Zucchini is a unique and healthy take on the classic potato dish. It has only 5 ingredients and easy to prepare!
Gnocchi joins sliced zucchini in a quick and easy garlic-Parmesan sauce in this weeknight dish that takes just about 20 minutes to make.
در سالهای اخیر به منظور افزایش سلامت بومنظام های زراعی، استفاده از نهاده های بوم سازگار بهویژه در زمینهی تولید گیاهان دارویی بیشتر مورد توجه قرار گرفته است. به منظور بررسی اثر کودهای آلی و بیولوژیک بر خصوصیات کمی و کیفی کدو پوست کاغذی (Cucurbita pepo L.) در شرایط کشت و عدم کشت گیاهان پوششی، آزمایشی در سال زراعی 89-1388 در مزرعه تحقیقاتی دانشکدهی کشاورزی دانشگاه فردوسی مشهد، به صورت کرتهای دوبار خردشده در قالب طرح پایهی بلوکهای کامل تصادفی با سه تکرار انجام شد. بقایای یکسالهی چهار نوع کود آلی مختلف (گاوی، گوسفندی، مرغی و ورمیکمپوست) در کرتهای اصلی، تلقیح و عدم ...
It feels like there is a sudden coating on my lips, and that they swell. I dont think they actually are swelling, and if they do, it doesnt seem to last long. Its weird to describe and not painful. I eat them anyway because I am not sure if this is normal ...
菌生真菌為一種真菌分別以寄生、片利共生、腐生的形式生長在另一種真菌上,目前仍無法明確得知其菌生菌與宿主間的相互作用。子囊菌門當中的肉座菌目(Hypocreales),有很大一部分為菌生真菌的種類。本研究以隨機採集的方式,鑑定及分析北台灣菌生真菌種類,同時記錄台灣新的真菌種類。利用真菌型態學及DNA分子技術鑑定出真菌種類;比較真菌培養於玉米瓊酯培養基及自然基質下,真菌型態的不同,同時利用Internal transcribed spacer (ITS),Large subunit (LSU) rDNA或 small subunit (SSU) rDNA 鑑定真菌種類。此研究採集及鑑定的菌生真菌有14種,分別是: Acremonium sp., Acremonium citrinum, Cladobotryum semicirculare, Hypomyces chrysospermus, Hypomyces albidus, Hypomyces corticiicola, Lecancillium psallioate, Nectriopsis rexiana‏, Ramularia coleosporii, Ramularia sp.?, Simplicillium lanosoniveum, Sphaerostilbella sp., Trichoderma ...
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dehydroascorbic acid 490-83-5 NMR spectrum, dehydroascorbic acid H-NMR spectral analysis, dehydroascorbic acid C-NMR spectral analysis ect.
Ascorbate and glutathione (GSH) are the primary water-soluble antioxidants in the CNS. Oxidative stress, sometimes indicated by loss of these antioxidants, has been linked to several clinical and experimental conditions, including cerebral ischemia. These conditions are also gender-linked, with greater incidence or severity in males than females. To test whether there are gender differences in oxidant/antioxidant regulation, we determined basal levels of ascorbate and GSH in rat brain and their loss after 1 h decapitation ischemia. We found that ascorbate levels in male rat brain were 7-10% higher than in females, depending on region, whereas GSH levels were gender-independent. Significant ascorbate loss (up to 12%) occurred in males during ischemia, with a regional pattern of cerebellum | hippocampus | prefrontal cortex. Loss of ascorbate in females was not significant in any region. By contrast, loss of GSH was significant in both males and females. Greater loss of GSH than ascorbate was in agreement
Sigma-Aldrich offers Sigma-L2157, Laccase from Rhus vernicifera for your research needs. Find product specific information including CAS, MSDS, protocols and references.
Cucurbita pepo belongs to the Cucurbitaceae family. The Zucchini types rank among the highest-valued vegetables worldwide, and other C. pepo and related Cucurbita spp., are food staples and rich sources of fat and vitamins. A broad range of genomic tools are today available for other cucurbits that have become models for the study of different metabolic processes. However, these tools are still lacking in the Cucurbita genus, thus limiting gene discovery and the process of breeding. We report the generation of a total of 512,751 C. pepo EST sequences, using 454 GS FLX Titanium technology. ESTs were obtained from normalized cDNA libraries (root, leaves, and flower tissue) prepared using two varieties with contrasting phenotypes for plant, flowering and fruit traits, representing the two C. pepo subspecies: subsp. pepo cv. Zucchini and subsp. ovifera cv Scallop. De novo assembling was performed to generate a collection of 49,610 Cucurbita unigenes (average length of 626 bp) that represent the first
Cucurbita pepo pepocin protein: type 1 ribosome-inactivating protein isolated from the fruit of Cucurbita pepo; amino acid sequence in first source
Why do we need Sodium Ascorbate?. The GLO Gene is absent in many people. Scientists believe that this happened around 60 million years ago. The absence of this gene makes us unable to biosynthesize the specific enzyme L- gulonolactone oxidase. This is an end-stage liver enzyme that bio-converts blood glucose to Ascorbate; without it, our bodies cannot produce any Ascorbate. In the long term, the lack of Ascorbate causes our bones to dissolve and teeth to fall out; in other words, sub-clinical scurvy and a tendency to develop cancer.. Ascorbic Acid is produced in large quantities in almost all animals, e.g. 6 grams a day in goat, or up to 100grams daily if the goat is stressed. But it is not the Ascorbic Acid our body requires, rather a SALT of Ascorbic Acid. A daily supplementation of Ascorbate several times a day is a simple way to make up for this genetic defect.. Robert F. Cathcart, MD another medical pioneer who has had great success with Ascorbate, successfully treating over 25,000 patients ...
Zucchinis are good for a beginning garden and grow well in most climates. Theyre a natural for summer gardens, usually ending the season with glorious glut. They are extremely easy to grow, says Rich Merrill of The Gardeners Table. He recommends eating the zucchini when small, not letting them grow too large. Zucchini is available in most supermarkets year-round. When picking, look for small to medium zucchini with no soft or extra dark spots. It should be firm, not withered or dried out. Its a summer squash, so take care not to knick its gentle skin while handling. You can easily peel the skin off with a knife, but it tastes great left on, softening along with the flesh in cooking. If you stir-fry zucchini, do it quickly so that it remains intact. Long slow cooking will surely turn it to goo. One cup of sliced raw zucchini is about 15 calories. It is high in potassium and vitamin A. ...
Ascorbate peroxidase is a bifunctional peroxidase that catalyzes the H(2)O(2)-dependent oxidation of both ascorbate and various aromatic substrates. The ascorbate binding site was recently identified as being close to the gamma-heme edge [Sharp, K. H., Mewies, M., Moody, P. C. E., and Raven, E. L. (2003)Nat. Struct. Biol. 10, 303-307]. In this work, the X-ray crystal structure of recombinant soybean cytosolic ascorbate peroxidase (rsAPX) in complex with salicylhydroxamic acid (SHA) has been determined to 1.46 A. The SHA molecule is bound close to the delta-heme edge in a cavity that connects the distal side of the heme to the surface of the protein. There are hydrogen bonds between the phenolic hydroxide of the SHA and the main chain carbonyl of Pro132, between the carbonyl oxygen of SHA and the side chain guanadinium group of Arg38, and between the hydroxamic acid group and the indole nitrogen of Trp41. The structure provides the first information about the location of the aromatic binding site ...
Novel conglomerates consisting of saddle-distorted SnIV(DPP) (H2DPP = dodecaphenylporphyrin) complexes and μ3-O- centered and carboxylato-bridged trinuclear RuIII clusters linked by pyridine carboxylates were synthesized and characterized. SnIV-DPP complexes with Cl-, OH-, and 3- and 4-pyridine carboxylates ligands were characterized by spectroscopic methods and X-ray crystallography. Reactions of [Sn(DPP)-(pyridinecarboxylato)2] with trinuclear RuIII clusters gave novel conglomerates in moderate yields. The conglomerates are stable in solution as demonstrated by 1HNMR and electrospray ionization mass spectrometry (ESI-MS) measurements, which show consistent spectra with those expected from their structures, and also by electrochemical measurements, which exhibit reversible multistep redox processes. This stability stems from the saddle distortion of the DPP2-ligand to enhance the Lewis acidity of the SnIV center that strengthens the axial coordination of the linker. The fast intramolecular ...
Zucchini generally has a similar shape to a ridged cucumber. Zucchini is treated as a vegetable, however botanically it is an immature fruit, being the swollen ovary of the female zucchini flower. Zucchini are immature vegetable marrows.
The same neighbor who has given us tons of tomatoes just gave us two HUGE zucchinis. I swear, they are zucchini on steroids...they are MASSIVE. We were given these to use last year as well, and I remember being in shock of how large they were. Last year I made zucchini bread with them and this year I will do the same. However, I am going to use a different recipe. This time, I am going to use my Moms traditional Zucchini Bread recipe. It is amazing. All of my Moms sweet bread recipes are out of this world. Very moist, yet light on the oil, so flavorful and somewhat healthier than your typical sweet bread. Im not sure why I did not make this recipe last year...I guess I just forgot about it. Well folks, here it is ...
Sadly, there are a few zucchini plant diseases that you need to watch for in order to reap that bountiful harvest. Treating zucchini diseases often starts with soil preparation, crop rotation and a little pre-planning to minimize any potential issues. Learn more here.
Background: The use of high dose ascorbate infusions in cancer patients is widespread, but without evidence of efficacy. Several mechanisms whereby ascorbate could affect tumour progression have been proposed, including (i) the localised generation of cytotoxic quantities of H...
Cut zucchinis in half. Cut the round side 1/4 inch so it turns into a stable base. Scoop the center out with a spoon or a melon baller. Chop and save the insides of the zucchinis ...
Easy, delicious and healthy DMCs Zucchini Cake (1/12th of 13x9 pan) kec recipe from SparkRecipes. See our top-rated recipes for DMCs Zucchini Cake (1/12th of 13x9 pan) kec.
Zucchini has a slightly crisp texture and subtle, slightly sour flavour that goes well with all kinds of ingredients. Luckily in WA we can enjoy local zucchini for just about the whole year, coming from Carnarvon in winter and being produced locally in Wanneroo, Baldivis and the south west in winter. From time to time crossing over between regions there is insufficient quantity and some produce will be bought over from South Australia, although this is quite rare.. ...
Short on time? This yummy casserole takes advantage of convenience foods for a quick and easy dish to use up that ever-present zucchini. - Cheezy Crunchy Zucchini Bake Recipe - Quick Cooking at BellaOnline
Cut zucchini in half crossways, then cut lengthways into 1½ cm-thick chips. Pat zucchini dry with paper kitchen towel. Whisk egg in a shallow dish.
Freshly grated zucchini marinates with garlic and olive oil creating a simple and delicious sauce for angel hair pasta. The hot pasta cooks the zucchini in the bowl!
Full nutritional breakdown of the calories in Zucchini Casserole based on the calories and nutrition in each ingredient, including Canola Oil, Heart Smart Bisquick (mix only), Cheese, Mexican Fiesta Blend RF 2% (1 serv= 1/4 c = 4 TB), Zucchini, Egg substitute, liquid (Egg Beaters) and the other ingredients in this recipe.
Step-by-step tutorial on making these Tomato, Zucchini and Ricotta Tarts. tomato tarts, zucchini tarts, ladies luncheon menu, brunch menu, supper club recipes,
An heirloom acorn squash, with high yields. Good for storage, best eaten before the skin changes to yellow. Heirloom, before 1835. Seedlings should be
Miniature ornamental pumpkin, stores well for 8 months. Mature in 95 days. There is a great variety in shapes, colors and types of squash, making them fun
Calcium ascorbate is a buffered form of ascorbic acid (Vitamin C) and a source of the essential mineral, calcium. Get you calcium supplement from PureBulk.
Tender, versatile zucchini fresh from the garden can be paired with a range of foods for a tasty meal. The summer squash variety also benefits from being combined with another vegetable before it ...
Shop for Natures Way Buffered C-500 Mineral Ascorbate Capsules at Pick n Save. Find quality health products to add to your Shopping List or order online for Delivery or Pickup.
Nutrition Facts for Zucchini Pickled - Get a bar chart of the top 10 nutrients, and click to see an expanded list of over 151 nutrients, including amino acids.
Our oven-baked zucchini sticks taste every bit as good as their deep-fried brethren with only a fraction of the fat and calories. Serve with a side of your favorite marinara sauce for dipping.
This delicious zucchini and garlic soup has properties that help boost your immune system. Plus, it is low in calories and can be a great addition to your diet
Zucchini is abundant in the garden this time of year!  Although, the challenge sometimes can be figuring out what to do with all of it.  Dont overlo
Ester-C is a breakthrough patented formula supported with naturally occurring metabolites Metabolites help enhance the absorption of Vitamin C by your white blood cells an important part of your immune system Ester-Cs unique chemical-free manufacturing process neutralizes pH making it non-acidic and gentle on the stomach In a daily 1 000 dose Ester-X delivers the active immune Request A Quotation! ...
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If you are not reading this post in a feed reader or at http://agoodappetite.blogspot.com OR at http://agoodappetite.com then the site you are reading is illegally publishing copyrighted material. Contact me at katbaro AT yahoo DOT COM. All recipes, text and photographs in this post are the original creations & property of the author unless otherwise noted. © 2007-2011 Kathy ...
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These are so simple and so tasty. Not to mention super healthy. Some prep work is involved, but not too bad. You can use any cheese youd like. Sometimes I use
These species require external ascorbate supply, because they lack the biosynthetic enzyme L-gulonolactone oxidase. The ... Glucuronic acid is a precursor of ascorbic acid (vitamin C, formerly called as L-hexuronic acid). Ascorbate can be ...
Cytochrome c peroxidase is used as a soluble, easily purified model for cytochrome c oxidase. Ascorbate peroxidase Chloride ...
Ascorbate salts such as sodium ascorbate and calcium ascorbate are used in some dietary supplements. These release ascorbate ... l-gulonolactone oxidase, the last of the chain of four enzymes that synthesize vitamin C.[183][184] American biochemist Irwin ... However, taking vitamin C in the form of sodium ascorbate and calcium ascorbate may minimize this effect.[29] Other symptoms ... The most commonly used supplement compounds are ascorbic acid, sodium ascorbate and calcium ascorbate.[2] Vitamin C molecules ...
... and bacterial ascorbate oxidase, among others. While hephaestin shares 50% amino acid sequence identity with its serum ... a multicopper oxidase with ferroxidase activity". Biochemistry. 44 (45): 14725-31. doi:10.1021/bi051559k. hdl:2429/18540. PMID ... 610 nm consistent with other blue multicopper oxidases such as ceruloplasmin. By using ferrous ammonium sulfate as a substrate ... Hephaestin is a member of the family of copper oxidases that includes mammalian ceruloplasmin, yeast fet3 and fet5, ...
Thus, the two substrates of this enzyme are glutathione and protein disulfide, whereas its two products are glutathione disulfide and protein dithiol. This enzyme belongs to the family of oxidoreductases, specifically those acting on a sulfur group of donors with a disulfide as acceptor. The systematic name of this enzyme class is glutathione:protein-disulfide oxidoreductase. Other names in common use include glutathione-insulin transhydrogenase, insulin reductase, reductase, protein disulfide (glutathione), protein disulfide transhydrogenase, glutathione-protein disulfide oxidoreductase, protein disulfide reductase (glutathione), GSH-insulin transhydrogenase, protein-disulfide interchange enzyme, protein-disulfide isomerase/oxidoreductase, thiol:protein-disulfide oxidoreductase, and thiol-protein disulphide oxidoreductase. This enzyme participates in glutathione metabolism. ...
... s, which belong to the oxidoreductase family, are found in archaea, bacteria, fungi, and plants.[5] They are grouped as either assimilatory or dissimilatory sulfite reductases depending on their function, their spectroscopic properties, and their catalytic properties. This enzyme participates in selenoamino acid metabolism and sulfur assimilation. It employs two covalently coupled cofactors - an iron sulfur cluster and a siroheme - which deliver electrons to the substrate via this coupling.[6]. The systematic name of this enzyme class is hydrogen-sulfide:acceptor oxidoreductase. Other names in common use include assimilatory sulfite reductase, assimilatory-type sulfite reductase, and hydrogen-sulfide:(acceptor) oxidoreductase.. ...
Animals that can contract scurvy all lack the L-gulonolactone oxidase (GULO) enzyme, which is required in the last step of ... All species that do not synthesize ascorbate require it in the diet. Deficiency causes scurvy in humans, and somewhat similar ... Nishikimi M, Kawai T, Yagi K (October 1992). "Guinea pigs possess a highly mutated gene for L-gulono-gamma-lactone oxidase, the ... Nishikimi M, Yagi K (December 1991). "Molecular basis for the deficiency in humans of gulonolactone oxidase, a key enzyme for ...
Ascorbate salts such as sodium ascorbate and calcium ascorbate are used in some dietary supplements. These release ascorbate ... Nishikimi M, Kawai T, Yagi K (October 1992). "Guinea pigs possess a highly mutated gene for L-gulono-gamma-lactone oxidase, the ... However, taking vitamin C in the form of sodium ascorbate and calcium ascorbate may minimize this effect. Other symptoms ... In plants, vitamin C is a substrate for ascorbate peroxidase. This enzyme utilizes ascorbate to neutralize excess hydrogen ...
4-dihydroxyphenethylamine beta-oxidase 4-(2-aminoethyl) pyrocatechol beta-oxidase dopa beta-hydroxylase dopamine beta-oxidase ... DBH is a 290 kDa copper-containing oxygenase consisting of four identical subunits, and its activity requires ascorbate as a ... The three substrates of the enzyme are dopamine, vitamin C (ascorbate), and O2. The products are norepinephrine, ... The systematic name of this enzyme class is 3,4-dihydroxyphenethylamine, ascorbate:oxygen oxidoreductase (beta-hydroxylating). ...
... including that of ascorbate oxidase, and of other metallo-enzymes. These studies have thrown new light on electron-transfer ...
However, the trials at the Mayo Clinic were conducted with oral doses of ascorbate, not intravenous ascorbate as was done in ... Pauling argued that because humans lack a functional form of L-gulonolactone oxidase, an enzyme required to make vitamin C that ... Rath M, Pauling L (1990). "Hypothesis: lipoprotein(a) is a surrogate for ascorbate". Proc. Natl. Acad. Sci. USA. 87 (16): 6204- ... "Dietary Supplement Health and Education Act of 1994". Cameron E, Pauling L (October 1976). "Supplemental ascorbate in the ...
An example of a pseudogene is the gene for L-gulonolactone oxidase, a liver enzyme necessary for biosynthesis of L-ascorbic ... including humans which require ascorbic acid or ascorbate from food. The remains of this non-functional gene with many ... Nishikimi M, Kawai T, Yagi K (October 1992). "Guinea pigs possess a highly mutated gene for L-gulono-gamma-lactone oxidase, the ...
Ascorbate salts such as sodium ascorbate and calcium ascorbate are used in some dietary supplements. These release ascorbate ... He later developed the theory that humans possess a mutated form of the L-gulonolactone oxidase coding gene.[189] ... However, taking vitamin C in the form of sodium ascorbate and calcium ascorbate may minimize this effect.[28] Other symptoms ... The most commonly used supplement compounds are ascorbic acid, sodium ascorbate and calcium ascorbate.[2] Vitamin C molecules ...
1959年J·J·伯恩斯(J.J. Burns)表示,之所以一些哺乳動物易患壞血病,是由於自己的肝臟無法產生L-古洛糖酸-1,4-內酯氧化酶(英语:L-gulonolactone oxidase),這是連鎖四酶合成維生素C的最後步驟。[43][44]美國 ... into ascorbate in the mammalian liver. This liver metabolite, ascorbate, is produced in an unstressed goat for instance, at the ... 在人體內,維生素C是高效抗氧化劑,用來減輕抗壞血酸
The oxygen incorporated need not be derived from O2 with reduced ascorbate as one donor, and incorporation of one atom of ... In enzymology, an aminocyclopropanecarboxylate oxidase (EC 1.14.17.4) is an enzyme that catalyzes the chemical reaction 1- ... ascorbate, and O2, whereas its 5 products are ethylene, cyanide, dehydroascorbate, CO2, and H2O. This enzyme belongs to the ... Other names in common use include ACC oxidase, and ethylene-forming enzyme. As of late 2007, two structures have been solved ...
Messerschmidt A, Huber R (January 1990). "The blue oxidases, ascorbate oxidase, laccase and ceruloplasmin. Modelling and ... Ascorbate oxidase EC 1.10.3.3, a 3-domain enzyme found in higher plants. Nitrite reductase EC 1.7.2.1, a 2-domain enzyme ... Multicopper oxidases include: Ceruloplasmin EC 1.16.3.1 (ferroxidase), a 6-domain enzyme found in the serum of mammals and ... In addition to the above enzymes there are a number of other proteins that are similar to the multi-copper oxidases in terms of ...
... ascorbate oxidase, ascorbic oxidase, ascorbate dehydrogenase, L-ascorbic acid oxidase, AAO, L-ascorbate:O2 oxidoreductase, and ... In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction 2 L-ascorbate + O2 ⇌ {\ ... This enzyme participates in ascorbate metabolism. It employs one cofactor, copper. The systematic name of this enzyme class is ... L-ascorbate:oxygen oxidoreductase. Other names in common use include ascorbase, ascorbic acid oxidase, ...
In its patently pathological form, the effects of ascorbate deficiency are manifested as scurvy. It is likely that some level ... GULO belongs to a family of sugar-1,4-lactone oxidases, which also contains the yeast enzyme D-arabinono-1,4-lactone oxidase ( ... L-gulonolactone oxidase (EC 1.1.3.8) is an enzyme that produces vitamin C, but is non-functional in Haplorrhini (including ... L-gulonolactone oxidase deficiency is called "hypoascorbemia" and is described by OMIM (Online Mendelian Inheritance in Man) as ...
Bleeg HS, Christensen F (1982). "Biosynthesis of ascorbate in yeast. Purification of L-galactono-1,4-lactone oxidase with ... In enzymology, a L-galactonolactone oxidase (EC 1.3.3.12) is an enzyme that catalyzes the chemical reaction L-galactono-1,4- ... This enzyme is also called L-galactono-1,4-lactone oxidase. This enzyme participates in ascorbic acid and aldaric acid ... lactone + O2 ⇌ {\displaystyle \rightleftharpoons } L-ascorbate + H2O2 Thus, the two substrates of this enzyme are L-galactono-1 ...
White GA, Krupka RM (1965). "Ascorbic acid oxidase and ascorbic acid oxygenase of Myrothecium verrucaria". Arch. Biochem. ... Ascorbate 2,3-dioxygenase (EC 1.13.11.13) is an enzyme that catalyzes the chemical reaction ascorbate + O2 + H2O ⇌ {\ ... The systematic name of this enzyme class is ascorbate:oxygen 2,3-oxidoreductase (bond-cleaving). This enzyme is also called ... This enzyme participates in ascorbate and aldarate metabolism. It employs one cofactor, iron. ...
D-erythro-ascorbate + H2O2 Thus, the two substrates of this enzyme are D-arabinono-1,4-lactone and O2, whereas its two products ... In enzymology, a D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) is an enzyme that catalyzes the chemical reaction D-arabinono-1, ... Huh WK, Kim ST, Yang KS, Seok YJ, Hah YC, Kang SO (1994). "Characterisation of D-arabinono-1,4-lactone oxidase from Candida ... are D-erythro-ascorbate and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH ...
Amazingly, oxalate is a metabolite of ascorbate (vitamin C), and it is worth emphasizing that ascorbate is a direct precursor ... Many years later it was found to have oxalate oxidase activity generating 'antimicrobial' hydrogen peroxide from a substrate of ... oxalate oxidase 'one-very-tough-little- protein' was such an ancestor. This hypothesis stimulated a search for the evolutionary ... it was found to be an enzyme having oxalate oxidase (OXO) activity converting an oxalate substrate into carbon dioxide and ...
Citric and ascorbic acids target enzymes that degrade fruits and vegetables, e.g., mono/polyphenol oxidase which turns surfaces ... The most common antioxidant additives are ascorbic acid (vitamin C) and ascorbates. Thus, antioxidants are commonly added to ...
... into ascorbate in the mammalian liver. This liver metabolite, ascorbate, is produced in an unstressed goat for instance, at the ... Biochemical research in the 1950's showed that the lesion in scurvy is the absence of the enzyme, L-Gulonolactone oxidase (GLO ... Effect of ascorbate deficiency on the in situ activity of gamma-butyrobetaine hydroxylase" (PDF). J Biol Chem. 259 (17): 10764- ... Proctor P (1970). "Similar functions of uric acid and ascorbate in man?". Nature. 228 (5274): 868. doi:10.1038/228868a0. PMID ...
Ether lipid oxidase (alkylglycerol monooxygenase, AGMO) catalyses the conversion of 1-alkyl-sn-glycerol to 1-hydroxyalkyl-sn- ... Research shows that ascorbic acid (also known as ascorbate or vitamin C) can reduce BH3 radical into BH4, preventing the BH3 ...
Xanthine oxidase is a large enzyme whose active site consists of the metal molybdenum bound to sulfur and oxygen. Within cells ... Proctor, P. (November 1970). "Similar functions of uric acid and ascorbate in man?". Nature. 228 (5274): 868. Bibcode:1970Natur ... Xanthine oxidase is an enzyme which catalyzes the formation of uric acid from xanthine and hypoxanthine, which in turn are ... Pacher, P; Nivorozhkin, A; Szabó, C (2006). "Therapeutic effects of xanthine oxidase inhibitors: Renaissance half a century ...
NADPH Oxidase Family 5.B.2 The Eukaryotic Cytochrome b561 (Cytb561) Family 5.B.3 The Geobacter Nanowire Electron Transfer (G- ... Family 4.A.7 The PTS L-Ascorbate (L-Asc) Family 4.B.1 The Nicotinamide Ribonucleoside (NR) Uptake Permease (PnuC) Family 4.C.1 ... Superfamily 3.D.4 Proton-translocating Cytochrome Oxidase (COX) Superfamily 3.D.5 The Na+-translocating NADH:Quinone ...
Ferrous-oxidase mediated transport systems exist in order to transport specific ions opposed to DMT1, which does not have ... of iron being reduced by ferrireductases that are present on the cell surface or by dietary reductants such as ascorbate ( ... So in anaerobic environments the oxidase would not be able to function thus another means of iron uptake is necessary. Toxic ...
... and can effectively scavenge NO at oxygen levels far below the saturation of cytochrome c oxidase. In the course of the ... "Nitric oxide scavenging by barley hemoglobin is facilitated by a monodehydroascorbate reductase-mediated ascorbate reduction of ...
... s contain oxidative enzymes, such as D-amino acid oxidase and uric acid oxidase.[9] However the last enzyme is absent ... the components of the ascorbate-glutathione cycle, and the NADP-dehydrogenases of the pentose-phosphate pathway. It has been ...
Calcium ascorbate (Vitamin C). *Kalsiyum benzoate - koruyucu. *Kalsiyum bisulfite - koruyucu, antioksidan. *Kalsiyum carbonates ... Glucose oxidase - antioksidan. *Glucose syrup - tatlandırıcı. *Glutamat -. *Glutamik asid - aroma arttırıcı. *Gluten - ...
ascorbic acid, sodium ascorbate cheese, chips E321 butylated hydroxytoluene, butylated hydroxyanisole also used in food ... Citric and ascorbic acids target enzymes that degrade fruits and vegetables, e.g., mono/polyphenol oxidase which turns surfaces ... The most common antioxidant additives are ascorbic acid (vitamin C) and ascorbates.[9] Thus, antioxidants are commonly added to ...
... such as ascorbate in the glutathione-ascorbate cycle, glutathione peroxidases and glutaredoxins, as well as reacting directly ... Uric acid (UA) is an antioxidant oxypurine produced from xanthine by the enzyme xanthine oxidase, and is an intermediate ... 2 Fe3+ + Ascorbate → 2 Fe2+ + Dehydroascorbate 2 Fe2+ + 2 H2O2 → 2 Fe3+ + 2 OH· + 2 OH−. The relative importance of the ... Besides ascorbate, medically important conditional pro-oxidants include uric acid and sulfhydryl amino acids such as ...
Reactive hydrogen peroxide (H2O2), the byproduct of oxalate oxidase reaction, can be neutralized by catalase. Alarm ... oxalate released from calcium oxalate crystals is converted to CO2 by an oxalate oxidase enzyme and the produced CO2 can ... Ascorbate. (vitamin C) δ-ALA Bile. pigments Hemes Cobalamins (vitamin B12) ...
1.14.17: reduced ascorbate. *Dopamine beta-hydroxylase. 1.14.18-19: other. *Tyrosinase ...
d-mannitol oxidase. *E number. *Mannitol dehydrogenase. *Mannitol dehydrogenase (cytochrome). *Mannitol-1-phosphatase ...
Formation of the collagen fibril: lysyl oxidase, an extracellular copper-dependent enzyme, produces the final step in the ... Peterkofsky, B (1991). "Ascorbate requirement for hydroxylation and secretion of procollagen: Relationship to inhibition of ... Multiple tropocollagen molecules form collagen fibrils, via covalent cross-linking (aldol reaction) by lysyl oxidase which ... Myllylä, R.; Majamaa, K.; Günzler, V.; Hanauske-Abel, H. M.; Kivirikko, K. I. (1984). "Ascorbate is consumed stoichiometrically ...
Ascorbate. (vitamin C) δ-ALA Bile. pigments Hemes Cobalamins (vitamin B12) ... Complex IV/Cytochrome c oxidase. *Coenzyme Q10 synthesis: COQ2. *COQ3. *COQ4. *COQ5 ...
Ang bitamina C(Vitamin C, L-ascorbic acid o L-ascorbate) ay isang mahalagang nutriento para sa mga tao at ilang mga species ng ... "Variation of l-gulonolactone oxidase activity in placental mammals". Comparative Biochemistry and Physiology B 67 (2): 195-204 ... Ang sintesis ng ascorbate ay isang nakabatay sa glycogenolisis na proseso. Sa mga reptilya at mga ibon, ang biosintesis ay ... Ang glucose na kailangan upang mag-sintesis ng ascorbate sa atay(sa mga mammal at mga ibon na nagsasagawa ng perching) ay ...
Ascorbate increases human oxaluria and kidney stone risk. „Journal of Nutrition". 135 (7), s. 1673-1677, 2005. PMID: 15987848 ( ... Gulonolactone oxidase is missing in teleost fish. The direct spectrophotometric assay. „Biol Chem Hoppe Seyler". 371 (3), s. ...
"The superoxide-generating oxidase of phagocytic cells. Physiological, molecular and pathological aspects". European Journal of ... ascorbate peroxidase, and glutathione peroxidase). The antioxidant superoxide dismutase catalyses the formation of hydrogen ... "Temporal mismatch between induction of superoxide dismutase and ascorbate peroxidase correlates with high H2O2 concentration in ...
PHD action normally requires oxygen and alpha-ketoglutarate as cosubstrates and ferrous iron and ascorbate as cofactors. ... Dihydrouracil oxidase. *Tetrahydroberberine oxidase. *Secologanin synthase. *Tryptophan alpha,beta-oxidase. *Pyrroloquinoline- ...
... into ascorbate in the mammalian liver. This liver metabolite, ascorbate, is produced in an unstressed goat for instance, at the ... "Biochemical research in the 1950's showed that the lesion in scurvy is the absence of the enzyme, L-Gulonolactone oxidase (GLO ... Effect of ascorbate deficiency on the in situ activity of gamma-butyrobetaine hydroxylase" (PDF). J Biol Chem 259 (17): 10764- ... Proctor P (1970). "Similar functions of uric acid and ascorbate in man?". Nature 228 (5274): 868. doi:10.1038/228868a0. பப்மெட் ...
Hypoxanthine is then oxidized to form xanthine and then uric acid through the action of xanthine oxidase. The other purine ... Ascorbate. (Vitamin C). δ-ALA. Bile. Pigments. Hemes. Cobalamins (Vitamin B12) ...
Vanlerberghe GC, McIntosh L (1997). "ALTERNATIVE OXIDASE: From Gene to Function". Annu. Rev. Plant Physiol. Plant Mol. Biol. 48 ... Calhoun MW, Thomas JW, Gennis RB (1994). "The cytochrome oxidase superfamily of redox-driven proton pumps". Trends Biochem. Sci ... Ito Y, Saisho D, Nakazono M, Tsutsumi N, Hirai A (1997). "Transcript levels of tandem-arranged alternative oxidase genes in ... Moore AL, Siedow JN (1991). "The regulation and nature of the cyanide-resistant alternative oxidase of plant mitochondria". ...
O ácido úrico (AU) é uma oxipurina produzida a partir da xantina pela enzima xantina oxidase, sendo um produto intermediário do ... Regulation and function of ascorbate peroxidase isoenzymes». Journal of Experimental Botany. 53 (372): 1305-19. PMID 11997377. ... Wu, X.; Lee, CC; Muzny, DM; Caskey, CT (1989). «Urate Oxidase: Primary Structure and Evolutionary Implications». Proceedings of ... embora o gene da urato oxidase esteja presente não é funcional, pelo que este estágio de processamento do AU não ocorre.[47][48 ...
... acidity regulator Glucose oxidase - antioxidant Glucose syrup - sweetener Glutamate - Glutamic acid - flavor enhancer Gluten - ... emulsifier Calcium ascorbate - antioxidant (water-soluble) Calcium aluminosilicate (calcium aluminium silicate) - anti-caking ... anti-caking agent Sodium ascorbate - antioxidant (water-soluble) Sodium benzoate - preservative Sodium bicarbonate - mineral ... anti-caking agent Potassium ascorbate - antioxidant (water-soluble) Potassium benzoate - preservative Potassium bicarbonate - ...
... ascorbate oxidase, ascorbic oxidase, ascorbate dehydrogenase, L-ascorbic acid oxidase, AAO, L-ascorbate:O2 oxidoreductase, and ... In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction 2 L-ascorbate + O2 ⇌ {\ ... This enzyme participates in ascorbate metabolism. It employs one cofactor, copper. The systematic name of this enzyme class is ... L-ascorbate:oxygen oxidoreductase. Other names in common use include ascorbase, ascorbic acid oxidase, ...
... ascorbate oxidase explanation free. What is ascorbate oxidase? Meaning of ascorbate oxidase medical term. What does ascorbate ... Looking for online definition of ascorbate oxidase in the Medical Dictionary? ... Effect of ascorbate oxidase overexpression on ascorbate recycling gene expression in response to agents imposing oxidative ... Ascorbate oxidase , definition of ascorbate oxidase by Medical dictionary https://medical-dictionary.thefreedictionary.com/ ...
Ascorbate oxidase involved in a redox system involving ascorbic acid (AsA) (PubMed:15883131, PubMed:27255930). The oxidation of ... IPR001117, Cu-oxidase. IPR011706, Cu-oxidase_2. IPR011707, Cu-oxidase_3. IPR033138, Cu_oxidase_CS. IPR002355, Cu_oxidase_ ... IPR001117, Cu-oxidase. IPR011706, Cu-oxidase_2. IPR011707, Cu-oxidase_3. IPR033138, Cu_oxidase_CS. IPR002355, Cu_oxidase_ ... IPR017760, L-ascorbate_oxidase_pln. Pfami. View protein in Pfam. PF00394, Cu-oxidase, 1 hit. PF07731, Cu-oxidase_2, 1 hit ...
... a simple and rapid method is described for detection of l-ascorbic acid by ascorbate oxidase immobilized onto polycarbonate ... Covalent attachment of ascorbate oxidase was confirmed by XPS studies. The immobilized-ascorbate oxidase shows higher pH, ... Herein, a simple and rapid method is described for detection of l-ascorbic acid by ascorbate oxidase immobilized onto ...
Ascorbate oxidase (AO) plays important roles in plant growth and development. Previously, we reported a cotton AO gene that ... ascorbate oxidase; light responsiveness; leaf senescence; H2O2 Gossypium hirsutum; ascorbate oxidase; light responsiveness; ... Ascorbate oxidase (AO) plays important roles in plant growth and development. Previously, we reported a cotton AO gene that ... "Genome-Wide Identification and Expression Analysis of the Ascorbate Oxidase Gene Family in Gossypium hirsutum Reveals the ...
X-ray structures and mechanistic implications of three functional derivatives of ascorbate oxidase from zucchini. Reduced, ... X-RAY STRUCTURES AND MECHANISTIC IMPLICATIONS OF THREE FUNCTIONAL DERIVATIVES OF ASCORBATE OXIDASE FROM ZUCCHINI: REDUCED-, ...
Ascorbate oxidase contains two paramagnetic Cu(ii) binding sites, type 1 (T1) and type 2 (T2) and in both sites the Cu(ii) is ... Ascorbate oxidase contains two paramagnetic Cu(II). binding sites. , type 1 (T1) and type 2 (T2) and in both sites the Cu(II). ... in ascorbate oxidase by high field pulse EPR. correlation spectroscopy. A. Potapov, I. Pecht and D. Goldfarb, Phys. Chem. Chem ...
Cucurbita species Ascorbate oxidase and Epstein-Barr Virus (LMP) ... Rabbit anti Cucurbita species Ascorbate oxidase Más información ... Eliminar este artículo Patógeno/Antígeno: Cucurbita species Ascorbate oxidase * Eliminar este artículo Patógeno/Antígeno: ...
In the g[unk] region of the spectrum of bleached ascorbate oxidase, a seven-line superhyperfine pattern is seen that is ... The superhyperfine splitting patterns in the g parallel region of the EPR spectra of native and bleached ascorbate oxidase show ... L-ascorbate:oxygen oxidoreductase, EC 1.10.3.3) and of a derivative of the enzyme having the type 1 (blue) copper reversibly ... it is proposed that the type 2 copper is part of the ascorbate binding site. ...
Ascorbate Oxidase, chemically modified from ,i,Cucurbita,/i, species, lyophilizate. Ascorbate Oxidase, chemically modified. ... Ascorbate Oxidase, chemically modified material number and pack size: Material Number. Pack Size. ... Use Ascorbate Oxidase, chemically modified, in a variety of diagnostic tests to eliminate the interference of ascorbic acid, ... Contaminants (+25°C, expressed as percentage of Ascorbate Oxidase activity):. Catalase: ≤0.2. Glutamate oxalacetate ...
Ascorbate Oxidase, chemically modified material number and pack size: Material Number. Pack Size. ... Use Ascorbate Oxidase, chemically modified, in a variety of diagnostic tests to eliminate the interference of ascorbic acid, ... Contaminants (+25°C, expressed as percentage of Ascorbate Oxidase activity):. Catalase: ≤0.2. Glutamate oxalacetate ... Nomenclature: L-ascorbate:oxygen oxidoreductase. Molecular weight: Approximately 140 kD. Isoelectric point: 5.0-6.0. Michaelis ...
2 L-ascorbate + O2↔ 2 dehydroascorbate + 2 H2O. Thus, the two subst ... a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction: ... ascorbase; ascorbic acid oxidase; ascorbate oxidase; ascorbic oxidase; ascorbate dehydrogenase; L-ascorbic acid oxidase; AAO; L ... ascorbate:O2 oxidoreductase; AA oxidase; EC 1.10.3.3; L-ascorbate oxidase ...
3. ascorbate oxidase. 1.11 Acting on H2 O2 as acceptor ... 9. galactose oxidase. 1.2 Acting on the CH--NH2 group of donors ... Gas chromatography/mass spectrometry determination of ascorbate and oxidation products thereof. US5340716 *. Jun 20, 1991. Aug ... Gas chromatography/mass spectrometry determination of ascorbate and oxidation products thereof. WO1998008097A1 *. Aug 22, 1997 ...
Ascorbate oxidase; Cholesterol esterase; Cholinesterase; Collagenase; DNase I; DNase II; Enterokinase; Glucose-6-phosphate ... Xanthine oxidase; Superoxide dismutase; Fibronectin; Restriction Endonucleases; Reverse transcriptase; M-MuLV; Monoclonal ... dehydrogenase; Glucose oxidase; Glucose Isomerase; Glutamate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; ...
SYN: ascorbate oxidase. ascorbate (as-kor′bat). A salt or ester of ascorbic acid. a. oxidase a copper-containing enzyme that ... Some forms of a. oxidase use NADP+ as well. Used as an antitumor enzyme. SYN: ascorbase. ascorbic acid (as-kor′bik). Used in ... May also indicate the reaction catalyzed, e.g., decarboxylase, oxidase. Enzymes named before the convention was established ...
Oxidase Dropper Pk. 50, OxiDrop™, Oxidase Test, Oxidase Reagent according to Gaby-Hadley A, for microbiology, Oxidase.. ... SIGMA Ascorbate Oxidase from Cucurbita sp. MilliporeSigma. lyophilized powder, 1,000-3,000 units/mg protein Synonym(s): L- ... Oxidase Dropper Pk. 50 BD. Oxidase Reagent Droppers are used in the Kovacs oxidase test as a qualitative reaction in the ... Bilirubin Oxidase from Myrothecium species MP Biomedicals. …oxidase system, Acyl coenzyme A oxidase system, and Glycerol-3- ...
... xanthine for xanthine oxidase; amino acid for amino acid oxidase; ascorbate for ascorbate oxidase; acyl-CoA for acyl-CoA ... oxidase; cholesterol for cholesterol oxidase; galactose for galactose oxidase; oxalate for oxalate oxidase; and sarcosine for ... For example, a combination of the label and the substrate may include: glucose for glucose oxidase; ... sarcosine oxidase. The optical analysis may be performed using an enzyme label, such as peroxidase, β-galactosidase or alkaline ...
Specificity ASCORBATE OXIDASE, Target Species Plants, Host Rabbit, Format Purified, Isotypes Polyclonal IgG, Applicatio \ 0750- ... RABBIT ANTI CUCURBITA ASCORBATE OXIDASE, Product Type Polyclonal Antibody, ... RABBIT ANTI CUCURBITA ASCORBATE OXIDASE, Product Type Polyclonal Antibody, Specificity ASCORBATE OXIDASE, Target Species Plants ... Product name : RABBIT ANTI CUCURBITA ASCORBATE OXIDASE, Product Type Polyclonal Antibody, Specificity ASCORBATE OXIDASE, Target ...
Iron-rich SWCNT oxidized ascorbate to its radical. These in vitro results are in line with marked inflammatory response and ... Similarly, superoxide extracellularly generated by xanthine oxidase/xanthine yielded hydroxyl radicals. ...
Belongs to the iron/ascorbate-dependent oxidoreductase family.Curated. Family and domain databases. Gene3D Structural and ... 1-aminocyclopropane-1-carboxylate oxidase 2 (ACO2), 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) ... 1-aminocyclopropane-1-carboxylate oxidase 2 (EC:1.14.17.4*Search proteins in UniProtKB for this EC number. ... "Nucleotide sequence of a cDNA encoding 1-aminocyclopropane-1-carboxylate oxidase from senescing orchid petals.". Nadeau J.A., ...
Ascorbate Oxidase 1.10.3.3. Asparaginase 3.5.1.1. L-Aspartase 4.3.1.1. Aspartate Transcarbamylase 2.1.3.2. ...
... ascorbate oxidase from Cucurbita pepo L. and Cucurbita moschata Duchesne. The food enzyme l‐ascorbate: oxygen oxidoreductase ( ...
Ascorbate Oxidase Spatula. 10127531001. ATP, disodium salt, 10g. 10127523001. ATP, disodium salt, 5g. ...
a[CO2], ambient CO2; AO, ascorbate oxidase; APX, ascorbate peroxidase; AsA, L-Ascorbic acid; CO2, carbon dioxide; DAS, days ... ascorbate oxidase (DcAO), ascorbic acid peroxidase (DcAPX), monodehydroascorbate reductase (DcMDHAR), dehydroascorbate ... Imai, T., Ban, Y., Terakami, S., Yamamoto, T., and Moriguchi, T. (2009). L-ascorbate biosynthesis in peach: cloning of six L- ... Li, M. J., Ma, F. W., Liang, D., Li, J., and Wang, Y. L. (2010). Ascorbate biosynthesis during early fruit development is the ...
ABBREVIATIONS: HBOC, hemoglobin-based oxygen carrier; AA, ascorbic acid; LGO, l-gulonolactone oxidase; DHA, dehydroascorbate; ... Dunne J, Caron A, Menu P, Alayash AI, Buehler PW, Wilson MT, Silaghi-Dumitrescu R, Faivre B, and Cooper CE (2006) Ascorbate ... Effects of Endogenous Ascorbate on Oxidation, Oxygenation, and Toxicokinetics of Cell-Free Modified Hemoglobin after Exchange ... Effects of Endogenous Ascorbate on Oxidation, Oxygenation, and Toxicokinetics of Cell-Free Modified Hemoglobin after Exchange ...
The final concentrations of substrates were 10 mm glutamate + 10 mm malate, 20 mm α-glycerophosphate, or 3.3 mm ascorbate + 80 ... Complex IV (cytochrome c oxidase) activity was determined by oxidation of partially reduced cytochrome c (reduced OD − oxidized ... ascorbate plus TMPD. (c) Northern hybridization of RNA from third instar larvae of tko25t and Canton-S strains, probed ... cytochrome c oxidase). Citrate synthase activities showed no significant differences between the strains. (b) ATP synthesis ...
Ascorbate was measured as the difference ofA 265 before and after the addition of ascorbate oxidase. ... This suggests that the inhibition of Cyt coxidase activity by KCN inhibits ascorbate synthesis in situ due to a lack of an ... The inhibition of Cyt coxidase by KCN in complex IV blocks ascorbate production, possibly by the accumulation of electrons in ... 1997) Ascorbate synthesis and ascorbate peroxidase activity during the early stage of wheat germination. Physiol Plant 100:894- ...
Bleeg HS, Christensen F (1982). "Biosynthesis of ascorbate in yeast. Purification of L-galactono-1,4-lactone oxidase with ... In enzymology, a L-galactonolactone oxidase (EC 1.3.3.12) is an enzyme that catalyzes the chemical reaction L-galactono-1,4- ... This enzyme is also called L-galactono-1,4-lactone oxidase. This enzyme participates in ascorbic acid and aldaric acid ... lactone + O2 ⇌ {\displaystyle \rightleftharpoons } L-ascorbate + H2O2 Thus, the two substrates of this enzyme are L-galactono-1 ...
X-RAY STRUCTURES AND MECHANISTIC IMPLICATIONS OF THREE FUNCTIONAL DERIVATIVES OF ASCORBATE OXIDASE FROM ZUCCHINI: REDUCED-, ... ASCORBATE OXIDASE. Oligo-state. homo-dimer. SMTL ID. 1asp.2. Ligands. SUGAR (N-ACETYL-D-GLUCOSAMINE); COPPER (II) ION; ... X-RAY STRUCTURES AND MECHANISTIC IMPLICATIONS OF THREE FUNCTIONAL DERIVATIVES OF ASCORBATE OXIDASE FROM ZUCCHINI: REDUCED-, ...
  • In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction 2 L-ascorbate + O2 ⇌ {\displaystyle \rightleftharpoons } 2 dehydroascorbate + 2 H2O Thus, the two substrates of this enzyme are L-ascorbate and O2, whereas its two products are dehydroascorbate and H2O. (wikipedia.org)
  • The coordination environment of the type 2 (nonblue) copper in native ascorbate oxidase (L-ascorbate:oxygen oxidoreductase, EC 1.10.3.3) and of a derivative of the enzyme having the type 1 (blue) copper reversibly bleached has been examined by electron paramagnetic resonance (EPR) spectroscopy. (semanticscholar.org)
  • In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction:2 L-ascorbate + O2↔ 2 dehydroascorbate + 2 H2O. (creative-enzymes.com)
  • The food enzyme l‐ascorbate: oxygen oxidoreductase (EC 1.10.3.3) is extracted from fruit peels of Cucurbita pepo L. and Cucurbita moschata Duchesne by Nagase (Europa) GmbH. (europa.eu)
  • Ascorbate oxidase ( EC 1.10.3.3 ), a 3-domain enzyme found in higher plants. (embl.de)
  • Some of the antioxidant enzymes that are found to provide protection against the ROS are superoxide dismutase, catalase, peroxidase, glutathione peroxidase, and ascorbate oxidase (Rani et al. (thefreedictionary.com)
  • In plants, vitamin C is a substrate for ascorbate peroxidase . (wikipedia.org)
  • The results showed whereas, the shoot fresh and dry weights and photosynthetic rate in the treated group were decreased significantly compared to the control group at P ≤ 0.05, photochemical efficiency of photosystem II, total protein concentration, proline content and the activity of some antioxidant enzymes such as polyphenol oxidase, ascorbate peroxidase and catalase were increased in treated plants than control ones. (springer.com)
  • Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection. (nih.gov)
  • A major hydrogen peroxide detoxifying system in plant cells is the ascorbate-glutathione cycle, in which, ascorbate peroxidase (APX) enzymes play a key role catalyzing the conversion of H(2)O(2) into H(2)O, using ascorbate as a specific electron donor. (nih.gov)
  • CAT (catalase), SOD (Superoxide dismutase), GPX (Gluthatione peroxidase) and APX (Ascorbate peroxidase) enzymes, Reactive Oxygen Species (ROS). (nih.gov)
  • and ascorbate peroxidase, VcAPX) in rachis of treated and non-treated bunches. (csic.es)
  • In humans, vitamin C is a highly effective antioxidant , acting to lessen oxidative stress , a substrate for ascorbate peroxidase, [3] as well as an enzyme cofactor for the biosynthesis of many important biochemicals. (bionity.com)
  • Ascorbate peroxidase (APX), which is found in both the cytosol and the chloroplast of photosynthetic eukaryotes, is central to this photoprotective role. (elifesciences.org)
  • To prevent ascorbic acid from hindering the peroxide and its generated chromogen, we added ascorbate oxidase to the reagent. (thefreedictionary.com)
  • Covalent immobilization of ascorbate oxidase onto polycarbonate strip for l-ascorbic acid detection. (biomedsearch.com)
  • Herein, a simple and rapid method is described for detection of l-ascorbic acid by ascorbate oxidase immobilized onto polycarbonate strip pre-activated by 1-fluoro-2-nitro-4-azidobenzene in photochemical reaction. (biomedsearch.com)
  • Use Ascorbate Oxidase, chemically modified, in a variety of diagnostic tests to eliminate the interference of ascorbic acid, since ascorbic acid interferes with the Trinder reaction that is widely used for the colorimetric determination of analytes. (roche.com)
  • Guinea pigs, like humans, are incapable of endogenous production of ascorbic acid (AA) due to the evolutionary loss of functional hepatic l -gulonolactone oxidase (LGO). (aspetjournals.org)
  • Even when ascorbic acid is present at high concentrations, there are no data on the concentration of GL in plant tissues, and the integration of ascorbate synthesis, regeneration, and degradation is still not completely resolved. (plantphysiol.org)
  • In enzymology, a L-galactonolactone oxidase (EC 1.3.3.12) is an enzyme that catalyzes the chemical reaction L-galactono-1,4-lactone + O2 ⇌ {\displaystyle \rightleftharpoons } L-ascorbate + H2O2 Thus, the two substrates of this enzyme are L-galactono-1,4-lactone and O2, whereas its two products are L-ascorbic acid and H2O2. (wikipedia.org)
  • Ascorbate and ascorbic acid are both naturally present in the body, since the forms interconvert according to pH . (wikipedia.org)
  • L -ascorbate is a weak sugar acid structurally related to glucose which naturally occurs either attached to a hydrogen ion, forming ascorbic acid , or to a metal ion, forming a mineral ascorbate . (bionity.com)
  • EC Number: 2006-250-9 MDL Number: MFCD00599413 PubChem Substance ID: 24278141 Biochem/physiol Actions Inhibitor of xanthine oxidase and de novo pyrimidine biosynthesis. (thomassci.com)
  • Similarly, superoxide extracellularly generated by xanthine oxidase/xanthine yielded hydroxyl radicals. (cdc.gov)
  • Many et al 8 recently implicated increased xanthine oxidase expression. (ahajournals.org)
  • OxiDrop™, Oxidase Test is a liquid ready-to-use reagent that is used in procedures to detect cytochrome oxidase activity in bacteria. (thomassci.com)
  • the detection of the cytochrome oxidase activity of microorganisms within 1 minute. (thomassci.com)
  • The oxygen affinity of cbb 3 -Cox is about fivefold higher than that of aa 3 -type cytochrome oxidase ( aa 3 -Cox), which is probably important for respiration under lower oxygen concentrations ( 33 ). (asm.org)
  • By contrast, dehydroascorbate (DHA) enabled the mitochondria to gain ATP from sulfide oxidation even if the sulfide concentration far exceeded the threshold for inhibition of cytochrome oxidase. (biologists.org)
  • In isolated mitochondria, the energy-conserving sulfide oxidation pathway via cytochrome oxidase is inhibited by sulfide concentrations exceeding 10 μmoll -1 . (biologists.org)
  • The formation of the alternative biosynthetic pathways in photosynthetic eukaryotes uncoupled ascorbate synthesis from hydrogen peroxide production and likely contributed to the rise of ascorbate as a major photoprotective antioxidant. (elifesciences.org)
  • A large number of metal containing proteins, such as hemocyanin (a Cu metal containingprotein present in some invertebrate animals which helps them in the transport of O2;like hemoglobin in humans) retain metal ions in their active sites so a great interest is developed in such metal containing enzymes which are ureases, laccase, tyrosinase and ascorbate oxidase [5-9] containing enzyme urease (urea amidohydrolase EC 3. (thefreedictionary.com)
  • Ascorbate is also an electron donor for eight different enzymes and thereby plays an important role in a wide variety of biochemical processes, including collagen formation ( 15 ), norepinephrine biosynthesis ( 16 ), and mitochondrial fatty acid transport ( 17 ). (aacrjournals.org)
  • However, ascorbate is also an essential co-factor for a family of enzymes, the 2-oxoglutarate-dependent dioxygenases, which control many biological processes, ranging from the stress response to hypoxia, to the regulation of epigenetic enzymes. (otago.ac.nz)
  • These processes are important in cancer and we are investigating the contribution of ascorbate to cancer growth as a result of its co-factor activity for these enzymes. (otago.ac.nz)
  • We therefore hypothesized that hydrogen cyanide (HCN), a potent inhibitor of cytochrome c oxidase and of other metal-containing enzymes, might be responsible for the observed plant-killing effects. (asm.org)
  • [5] L -dehydroascorbate can then be reduced back to the active L -ascorbate form in the body by enzymes and glutathione . (bionity.com)
  • In addition to the above enzymes there are a number of other proteins that are similar to the multi-copper oxidases in terms of structure and sequence, some of which have lost the ability to bind copper. (embl.de)
  • To elucidate their role further, we studied the effects of AT 1 -AA on reactive oxygen species (ROS), NADPH oxidase expression, and nuclear factor-κB (NF-κB) activation. (ahajournals.org)
  • The double treatment of providing plants with reactive oxygen species scavenging compounds and overexpressing the alternative oxidase AOX1a led to a significant reduction of volatile-mediated toxicity. (asm.org)
  • Ascorbate also plays an antioxidant role in eukaryotes to help protect against reactive oxygen species (ROS) derived from metabolic activity. (elifesciences.org)
  • Low molecular weight antioxidants, such as ascorbate, glutathione, and tocopherol, are information-rich redox buffers that interact with numerous cellular components. (plantcell.org)
  • The ability of the ascorbate, glutathione, and tocopherol pools to act as redox buffers in plant cells is one of their most important attributes. (plantcell.org)
  • In nonquiescent cells under optimal conditions, large pools of glutathione and ascorbate are maintained in a highly reduced state, and buffer ROS that are continuously produced by oxidases or by electron transport components, such as FeS centers, semiquinones, or (as depicted) ferredoxin. (plantcell.org)
  • In the presence of the ROS scavengers glutathione (GSH) and ascorbate, isolated lugworm mitochondria rapidly oxidized up to 100 μmoll -1 sulfide with maximal oxygen consumption rates but did not produce any ATP in the process. (biologists.org)
  • Thylakoid- and stroma-localised APX removes H 2 O 2 produced by photosystem I through the activity of the ascorbate-glutathione cycle and this process may account for 10% of photosynthetic electron transport flow. (elifesciences.org)
  • Sulfide oxidation in the lugworm, Arenicola marina (L.), is most likely localized in the mitochondria, which can either produce ATP with sulfide as a substrate or detoxify it via an alternative oxidase. (biologists.org)
  • Under these conditions, salicylhydroxamic acid (SHAM), which is an inhibitor of the alternative oxidase of plant mitochondria, completely blocked oxygen consumption whereas inhibitors of complex III and IV had hardly any effect. (biologists.org)
  • The systematic name of this enzyme class is L-ascorbate:oxygen oxidoreductase. (wikipedia.org)
  • We suggest that AT 1 -AA through activation of NADPH oxidase could contribute to ROS production and inflammatory responses in preeclampsia. (ahajournals.org)
  • Current studies have exposed the capability of flavonoids to influence transportation of vitamin C around the body, and also assist to control the role of an enzyme called ascorbate oxidase , which converts vitamin C into non vitamin type. (thefreedictionary.com)
  • In regard to uric acid, it should be pointed out that vitamin C can interfere with the standard colorimetric uric acid assay, though excess ascorbate oxidase is usually added during the assays to prevent this. (thefreedictionary.com)
  • Stressful conditions of any kind greatly increase utilization of vitamin C. Ascorbate excreted in the urine drops markedly with stresses of any magnitude unless vitamin C is provided in large amounts. (doctoryourself.com)
  • Irwin Stone (10, 11, 12) pointed out the potential of vitamin C in the treatment of many diseases, the inability of humans to synthesize ascorbate, and the resultant condition hypoascorbemia. (doctoryourself.com)
  • L -gulonolactone oxidase ( EC 1.1.3.8 ) is an enzyme that produces vitamin C , but is non-functional in Haplorrhini (including humans), in some bats , and in guinea pigs . (wikipedia.org)
  • Unlike many animal cells, however, plant cells synthesize high concentrations of ascorbate (vitamin C), an additional hydrophilic redox buffer that provides robust protection against oxidative challenge. (plantcell.org)
  • Unlike other mammals, these do not synthesize ascorbate from their liver (ascorbate being the medical term for vitamin C). (lewrockwell.com)
  • A liver enzyme (gulonolactone oxidase) required to produce vitamin C is missing in humans due to a defect in the GULO gene. (lewrockwell.com)
  • Animals that synthesize ascorbate are known to maintain blood plasma at saturation levels regardless of health challenges, dramatically increasing vitamin C-production internally in response to physical or emotional stress. (lewrockwell.com)
  • DHA within red blood cells then converts back to ascorbate vitamin C . This recirculation of DHA back to ascorbate is what spares every human from dying of scurvy, which is a frank vitamin C deficiency (below 11.4 micromole blood concentration). (lewrockwell.com)
  • But let's return to the subject of stress and vitamin C. It is no wonder ascorbate levels are the highest in the adrenal glands. (lewrockwell.com)
  • Most of my current research revolves around determining the biological functions of vitamin C (ascorbate), which is widely considered to be an important natural antioxidant. (otago.ac.nz)
  • This research will help to inform the debate around the appropriate Nutrient Reference Value and Recommended Dietary Intake for vitamin C. Ascorbate acts as a cofactor for the enzymatic synthesis of mood and energy controlling hormones. (otago.ac.nz)
  • The flexible structure contains an enzyme, ascorbate oxidase, that converts vitamin C to dehydroascorbic acid, consuming oxygen in the process. (medgadget.com)
  • Vitamin C or L -ascorbate is an essential nutrient for higher primates, and a small number of other species. (bionity.com)
  • The pharmacophore of vitamin C is the ascorbate ion. (bionity.com)
  • [5] Most notably, humans have no capability to manufacture vitamin C. The cause of this phenomenon is that the last enzyme in the synthesis process, L -gulonolactone oxidase , cannot be made by the listed animals because the gene for this enzyme, Pseudogene ΨGULO, is defective. (bionity.com)
  • My research has shown that ascorbate affects white blood cell function, and we have an interest in the role of ascorbate in acute inflammatory diseases such as pneumonia and sepsis. (otago.ac.nz)
  • In a rabbit model, the administration of ascorbate aggravates damage, likely through the abrogation of the endogenous enzymatic antioxidant response triggered by short episodes of ischemia [ 10 ]. (hindawi.com)
  • In living organisms, ascorbate is an antioxidant , as it protects the body against oxidative stress , [4] and is a cofactor in several vital enzymatic reactions. (bionity.com)
  • Altered stomatal dynamics in ascorbate oxidase over-expressing tobacco plants suggest a role for dehydroascorbate signaling. (thefreedictionary.com)
  • Thus, the two substRates of this enzyme are L-ascorbate and O2, whereas its two products are dehydroascorbate and H2O. (creative-enzymes.com)
  • When L -ascorbate, which is a strong reducing agent, carries out its reducing function, it is converted to its oxidized form, L -dehydroascorbate . (bionity.com)
  • Apart from the specialized water-producing reactions catalyzed by specific oxidases, the initial product of this flux is superoxide, from which other ROS are subsequently produced ( Table 1 ). (plantcell.org)
  • Moreover, because the tocopherol redox couple has a more positive midpoint potential than that of the ascorbate pool, it increases even further the range of effective superoxide scavenging. (plantcell.org)
  • The stress of this lifestyle completely depletes the already dangerously low levels of ascorbate in their system. (newswithviews.com)
  • Restoring physiological levels of ascorbate slows tumor growth and moderates HIF-1 pathway activity in Gulo −/− mice. (otago.ac.nz)
  • Administration of ascorbate and deferoxamine reduces mortality and decreases lung injury through reduction of alveolar-capillary permeability, inflammation, and epithelial sloughing and lipid peroxidation. (pubmedcentralcanada.ca)
  • Our studies show that administration of ascorbate and deferoxamine reduced mortality and decreased lung injury through reduction of alveolar-capillary permeability, inflammation, and epithelial sloughing and lipid peroxidation. (pubmedcentralcanada.ca)
  • The respiration inhibitor antimycin A stimulated ascorbate biosynthesis, while cyanide inhibited both respiration and ascorbate production. (plantphysiol.org)
  • The acquisition of this additional iron depends on effluxed ascorbate and can be blocked by the DMT1 inhibitor ferristatin/NSC306711. (biochemj.org)
  • The pathway of ascorbate biosynthesis in plants is different from that in animals. (plantphysiol.org)
  • Biosynthesis of ascorbate in yeast. (wikipedia.org)
  • Although the main reactions of the ascorbate biosynthesis, recycling and translocation pathways have been characterized, the assignment of tomato genes to each enzymatic step of the entire network has never been reported to date. (deepdyve.com)
  • We demonstrate that isolated intact mitochondria synthesize ascorbate in the presence of GL. (plantphysiol.org)
  • Pauling observed that after the loss of endogenous ascorbate production, apo(a) and Lp(a) were greatly favored by evolution, acting as ascorbate surrogate, since the frequency of occurrence of elevated Lp(a) plasma levels in species that had lost the ability to synthesize ascorbate is great. (wikipedia.org)
  • Lack of this enzyme makes humans unable to synthesize ascorbate from blood glucose. (newswithviews.com)
  • Iron efflux is thought to be mediated by the metal transporter protein, IREG1/ferroportin1/MTP1, and oxidation of Fe(II) to Fe(III) prior to incorporation into fetal transferrin is carried out by the placental copper oxidase. (portlandpress.com)
  • Expression of IREG1 was not altered by iron deficiency, whereas copper oxidase activity was increased. (portlandpress.com)
  • Cytochrome cbb 3 -type oxidases are members of the heme copper oxidase superfamily and are composed of four subunits. (asm.org)
  • Most of these terminal oxidases belong to the heme copper oxidase superfamily ( 12 ), which is characterized by the presence of a six-coordinated low-spin heme and a unique binuclear center composed of a high-spin heme and a copper atom (Cu B ) within the subunit I ( 37 ). (asm.org)
  • Besides this common feature, the heme copper oxidase superfamily is quite diverse in terms of electron donor, oxygen affinity, subunit composition, and heme types. (asm.org)
  • Choline Oxidase from Alcaligenes sp. (thomassci.com)
  • has also been used in spectroscopic studies on the photoreaction of choline oxidase with covalently bound flavin. (thomassci.com)
  • The non-functional gulonolactone oxidase pseudogene (GULOP) was mapped to human chromosome 8p21 that corresponds to an evolutionarily conserved segment on either porcine chromosome 4 (SSC4) or 14 (SSC14). (wikipedia.org)
  • The loss of activity of the gene for L-gulonolactone oxidase (GULO) has occurred separately in the history of several species. (wikipedia.org)
  • L-gulonolactone oxidase deficiency is called " hypoascorbemia " [9] and is described by OMIM (Online Mendelian Inheritance in Man) [10] as "a public inborn error of metabolism", as it affects all humans. (wikipedia.org)
  • have hypothesized that the mutation of the GULOP (pseudogene that produces L-gulonolactone oxidase) so that it stopped producing GULO may have been of benefit to early primates by increasing uric acid levels and enhancing fructose effects on weight gain and fat accumulation. (wikipedia.org)
  • Human beings lack the L-gulonolactone oxidase enzyme (GLO). (newswithviews.com)
  • Many animal lineages, including primates, have become ascorbate auxotrophs due to the loss of the terminal enzyme in their biosynthetic pathway, l -gulonolactone oxidase (GULO). (elifesciences.org)
  • Coordination environment and fluoride binding of type 2 copper in the blue copper protein ascorbate oxidase. (semanticscholar.org)
  • Lung tissue ascorbate correlated inversely with BAL protein as well as with the number of neutrophils and epithelial cells. (pubmedcentralcanada.ca)
  • norE encodes a protein with five putative transmembrane alpha-helices and has similarity to CoxIII, the third subunit of the aa3-type cytochrome-c oxidases. (tcdb.org)
  • COPPER is essential as a cofactor for serum ceruloplasmin, an oxidase necessary for proper formation of the iron carrier protein, transferrin. (drugs.com)
  • Nitrite reductase and NO reductase (with succinate or ascorbate as substrates) and nitrous oxide reductase (with succinate as substrate) activities were not detected in these mutant strains. (tcdb.org)
  • and several other oxidases with specific substrates such as ascorbate, bilirubin or copper. (malariaworld.org)
  • Our data suggest that an Fe(III)-uptake pathway predominates when astrocytes are ascorbate-deficient, but that in ascorbate-replete astrocytes, at least half of the accumulated iron is initially reduced by effluxed ascorbate and then imported by DMT1. (biochemj.org)
  • By integrating bioinformatics approaches, omics resources and transcriptome collections today available for tomato, this study provides an overview on the architecture of the ascorbate pathway. (deepdyve.com)
  • Like many other bacterial members of this superfamily, the cytochrome cbb 3 -type oxidase contains a fourth, non-cofactor-containing subunit, which is termed CcoQ. (asm.org)
  • The cofactor activities of ascorbate are likely to influence many biological processes that impact on health and disease, and our aim is to understand the extent of this involvement in the functioning of the immune system. (otago.ac.nz)
  • exhibits internal sequence homology that appears to have evolved from the triplication of a Cu-binding domain similar to that of laccase and ascorbate oxidase. (embl.de)
  • Laccase ( EC 1.10.3.2 ) (urishiol oxidase), a 3-domain enzyme found in fungi and plants, which oxidizes different phenols and diamines. (embl.de)
  • 100 [micro]l of pH-adjusted sample extract was mixed with 900 [micro]l assay buffer, and the change in absorbance at 265 nm was recorded after reaction with 2 units ascorbate oxidase (Logan et al. (thefreedictionary.com)
  • ascorbate synthesis is a glycogenolysis-dependent process. (bionity.com)
  • Effect of ascorbate oxidase overexpression on ascorbate recycling gene expression in response to agents imposing oxidative stress. (thefreedictionary.com)
  • Oxidase Reagent Droppers are used in the Kovacs oxidase test as a qualitative reaction in the identification of nonfermenters and miscellaneous gram-negative bacteria. (thomassci.com)
  • For testing the oxidase reaction, contains tetramethyl-p-phenylenediamine. (thomassci.com)
  • BBL DrySlide Oxidase is used for determining the oxidase reaction of bacteria. (thomassci.com)
  • Suppressed expression of the apoplastic ascorbate oxidase gene increases salt tolerance in tobacco and Arabidopsis plants. (nih.gov)
  • Tenders are invited for Biochemistry:Uric Acid Uricase Mthd Liq Stable Reagent With Ascorbate Oxidase ,Direct Linearity Or 25 Mg/Dl 6X100ml Kits Should Mention Reagent Compositionwith Conc. (thefreedictionary.com)
  • This enzyme participates in ascorbate metabolism. (wikipedia.org)
  • All mammals except primates, guinea pigs, and humans use glucose to create ascorbate when placed in stressful situations. (newswithviews.com)
  • Klenner found that viral diseases could be cured by intravenous sodium ascorbate in amounts up to 200 grams per 24 hours. (doctoryourself.com)
  • Ascorbate salts such as sodium ascorbate and calcium ascorbate are used in some dietary supplements. (wikipedia.org)
  • Patients taking sodium and potassium ascorbate avoid this problem. (newswithviews.com)
  • Since humans are ascorbate auxotrophs, enhancing the nutritional quality of a widely consumed vegetable like tomato is a desirable goal. (deepdyve.com)
  • Multicopper oxidases consist of 2, 3 or 6 of these homologous domains, which also share homology to the cupredoxins azurin and plastocyanin. (embl.de)
  • This entry represents multicopper oxidase type 1 (blue) domains. (embl.de)
  • However, if doses of ascorbate are not provided to satisfy this potential draw on the nutrient, first local tissues involved in the disease, then the blood, and then the body in general become deplete of ascorbate (ANASCORBEMIA and ACUTE INDUCED SCURVY). (doctoryourself.com)
  • [11] In its patently pathological form, the effects of ascorbate deficiency are manifested as scurvy . (wikipedia.org)
  • At higher sulfide concentrations, the electrons are transferred to oxygen via an alternative terminal oxidase (AOX), presumably resembling the enzyme found in plant mitochondria as it is equally cyanide insensitive and also inhibited by salicylhydroxamic acid (SHAM) ( Völkel and Grieshaber, 1996 ). (biologists.org)
  • Humans suffer severe damage from stress unless they are being provided supplemental ascorbate, as their needs may rise to 30 to 100 grams of ascorbate daily. (newswithviews.com)
  • Total foliar ascorbate and GLDH activity decreased with leaf age. (plantphysiol.org)
  • The intensity of the colored response is less subject to ascorbate interference due to the activity of the iodate ion. (google.com)
  • In the present study, we analyzed the role of CcoQ on the stability and activity of Rhodobacter capsulatus cbb 3 -type oxidase. (asm.org)
  • In the absence of CcoQ, cbb 3 -type oxidase activity is significantly reduced, irrespective of the growth conditions. (asm.org)
  • Collectively, our data demonstrated that in R. capsulatus CcoQ was required for optimal cbb 3 -type oxidase activity because it stabilized the interaction of CcoP with the CcoNO core complex, leading subsequently to the formation of the active 230-kDa cbb 3 -type oxidase complex. (asm.org)
  • Scorbutic type bone changes seen in infants fed exclusively with copper-poor cow's milk are believed due to decreased activity of ascorbate oxidase, a cuproenzyme. (drugs.com)
  • The presence of ascorbate is required for a range of essential metabolic reactions in all animals and plants. (bionity.com)
  • Aerobic and facultative aerobic bacteria usually contain multiple respiratory oxidases that facilitate their adaptation to different environmental O 2 concentrations. (asm.org)