Ascorbate Oxidase: An enzyme that converts ascorbic acid to dehydroascorbic acid. EC 1.10.3.3.Laccase: A copper-containing oxidoreductase enzyme that catalyzes the oxidation of 4-benzenediol to 4-benzosemiquinone. It also has activity towards a variety of O-quinols and P-quinols. It primarily found in FUNGI and is involved in LIGNIN degradation, pigment biosynthesis and detoxification of lignin-derived products.Cucurbita: A plant genus of the family CUCURBITACEAE, order Violales, subclass Dilleniidae, which includes pumpkin, gourd and squash.Ascorbic Acid: A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.Toxicodendron: A genus (formerly part of Rhus genus) of shrubs, vines, or trees that yields a highly allergenic oleoresin which causes a severe contact dermatitis (DERMATITIS, TOXICODENDRON). The most toxic species are Toxicodendron vernix (poison sumac), T. diversilobum (poison oak), and T. radicans (poison ivy). T. vernicifera yields a useful varnish from which certain enzymes (laccases) are obtained.Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Pulse Radiolysis: Use of a pulse of X-rays or fast electrons to generate free radicals for spectroscopic examination.Oxidoreductases: The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)Acremonium: A mitosporic fungal genus with many reported ascomycetous teleomorphs. Cephalosporin antibiotics are derived from this genus.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Electron Spin Resonance Spectroscopy: A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.Copper: A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.Electroporation: A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.Superoxides: Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides.Superoxide Dismutase: An oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. EC 1.15.1.1.Pyrogallol: A trihydroxybenzene or dihydroxy phenol that can be prepared by heating GALLIC ACID.Nitric Oxide: A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.Peroxynitrous Acid: A potent oxidant synthesized by the cell during its normal metabolism. Peroxynitrite is formed from the reaction of two free radicals, NITRIC OXIDE and the superoxide anion (SUPEROXIDES).Nitric Oxide Synthase: An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Kinetics: The rate dynamics in chemical or physical systems.Vasodilation: The physiological widening of BLOOD VESSELS by relaxing the underlying VASCULAR SMOOTH MUSCLE.Liposomes: Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antigen-Antibody Complex: The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Acute-Phase Reaction: An early local inflammatory reaction to insult or injury that consists of fever, an increase in inflammatory humoral factors, and an increased synthesis by hepatocytes of a number of proteins or glycoproteins usually found in the plasma.Complement System Proteins: Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).Antigens: Substances that are recognized by the immune system and induce an immune reaction.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Complement Activation: The sequential activation of serum COMPLEMENT PROTEINS to create the COMPLEMENT MEMBRANE ATTACK COMPLEX. Factors initiating complement activation include ANTIGEN-ANTIBODY COMPLEXES, microbial ANTIGENS, or cell surface POLYSACCHARIDES.Stevens-Johnson Syndrome: Rare cutaneous eruption characterized by extensive KERATINOCYTE apoptosis resulting in skin detachment with mucosal involvement. It is often provoked by the use of drugs (e.g., antibiotics and anticonvulsants) or associated with PNEUMONIA, MYCOPLASMA. It is considered a continuum of Toxic Epidermal Necrolysis.Rehydration Solutions: Fluids restored to the body in order to maintain normal water-electrolyte balance.Chemistry, Pharmaceutical: Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use.Fluid Therapy: Therapy whose basic objective is to restore the volume and composition of the body fluids to normal with respect to WATER-ELECTROLYTE BALANCE. Fluids may be administered intravenously, orally, by intermittent gavage, or by HYPODERMOCLYSIS.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Dehydration: The condition that results from excessive loss of water from a living organism.Excipients: Usually inert substances added to a prescription in order to provide suitable consistency to the dosage form. These include binders, matrix, base or diluent in pills, tablets, creams, salves, etc.Drug Compounding: The preparation, mixing, and assembling of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814)Delayed-Action Preparations: Dosage forms of a drug that act over a period of time by controlled-release processes or technology.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Proteomics: The systematic study of the complete complement of proteins (PROTEOME) of organisms.Tablets: Solid dosage forms, of varying weight, size, and shape, which may be molded or compressed, and which contain a medicinal substance in pure or diluted form. (Dorland, 28th ed)Click Chemistry: Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.Protease Inhibitors: Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).Protein Stability: The ability of a protein to retain its structural conformation or its activity when subjected to physical or chemical manipulations.HIV Protease Inhibitors: Inhibitors of HIV PROTEASE, an enzyme required for production of proteins needed for viral assembly.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Carbon Dioxide: A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.Daucus carota: A plant species of the family APIACEAE that is widely cultivated for the edible yellow-orange root. The plant has finely divided leaves and flat clusters of small white flowers.Cucumis melo: A plant species of the family CUCURBITACEAE, order Violales, subclass Dilleniidae known for the melon fruits with reticulated (net) surface including cantaloupes, honeydew, casaba, and Persian melons.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Plants, Edible: An organism of the vegetable kingdom suitable by nature for use as a food, especially by human beings. Not all parts of any given plant are edible but all parts of edible plants have been known to figure as raw or cooked food: leaves, roots, tubers, stems, seeds, buds, fruits, and flowers. The most commonly edible parts of plants are FRUIT, usually sweet, fleshy, and succulent. Most edible plants are commonly cultivated for their nutritional value and are referred to as VEGETABLES.Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Vegetables: A food group comprised of EDIBLE PLANTS or their parts.Carbon: A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.Reoperation: A repeat operation for the same condition in the same patient due to disease progression or recurrence, or as followup to failed previous surgery.Prosthesis Failure: Malfunction of implantation shunts, valves, etc., and prosthesis loosening, migration, and breaking.Hip Prosthesis: Replacement for a hip joint.Antigens, CD8: Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.Prosthesis Design: The plan and delineation of prostheses in general or a specific prosthesis.Antigens, CD3: Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).CD40 Ligand: A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.Knee Prosthesis: Replacement for a knee joint.Prosthesis-Related Infections: Infections resulting from the implantation of prosthetic devices. The infections may be acquired from intraoperative contamination (early) or hematogenously acquired from other sites (late).Antigens, CD40: A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.

Azide binding to the trinuclear copper center in laccase and ascorbate oxidase. (1/79)

Azide binding to the blue copper oxidases laccase and ascorbate oxidase (AO) was investigated by electron paramagnetic resonance (EPR) and pulsed electron-nuclear double resonance (ENDOR) spectroscopies. As the laccase : azide molar ratio decreases from 1:1 to 1:7, the intensity of the type 2 (T2) Cu(II) EPR signal decreases and a signal at g approximately 1.9 appears. Temperature and microwave power dependent EPR measurements showed that this signal has a relatively short relaxation time and is therefore observed only below 40 K. A g approximately 1.97 signal, with similar saturation characteristics was found in the AO : azide (1:7) sample. The g < 2 signals in both proteins are assigned to an S = 1 dipolar coupled Cu(II) pair whereby the azide binding disrupts the anti-ferromagnetic coupling of the type 3 (T3) Cu(II) pair. Analysis of the position of the g < 2 signals suggests that the distance between the dipolar coupled Cu(II) pair is shorter in laccase than in AO. The proximity of T2 Cu(II) to the S = 1 Cu(II) pair enhances its relaxation rate, reducing its signal intensity relative to that of native protein. The disruption of the T3 anti-ferromagnetic coupling occurs only in part of the protein molecules, and in the remaining part a different azide binding mode is observed. The 130 K EPR spectra of AO and laccase with azide (1:7) exhibit, in addition to an unperturbed T2 Cu(II) signal, new features in the g parallel region that are attributed to a perturbed T2 in protein molecules where the anti-ferromagnetic coupling of T3 has not been disrupted. While these features are also apparent in the AO : azide sample at 10 K, they are absent in the EPR spectra of the laccase : azide sample measured in the range of 6-90 K. Moreover, pulsed ENDOR measurements carried out at 4.2 K on the latter exhibited only a reduction in the intensity of the 20 MHz peak of the 14N histidine coordinated to the T2 Cu(II) but did not resolve any significant changes that could indicate azide binding to this ion. The lack of T2 Cu(II) signal perturbation below 90 K in laccase may be due to temperature dependence of the coupling within the trinuclear : azide complex.  (+info)

Homology modeling of the multicopper oxidase Fet3 gives new insights in the mechanism of iron transport in yeast. (2/79)

Fet3, the multicopper oxidase of yeast, oxidizes extracellular ferrous iron which is then transported into the cell through the permease Ftr1. A three-dimensional model structure of Fet3 has been derived by homology modeling. Fet3 consists of three cupredoxin domains joined by a trinuclear copper cluster which is connected to the blue copper site located in the third domain. Close to this site, which is the primary electron acceptor from the substrate, residues for a potential iron binding site could be identified. The surface disposition of negatively charged residues suggests that Fet3 can translocate Fe(3+) to the permease Ftr1 through a pathway under electrostatic guidance.  (+info)

Effects of superoxide anion generators and thiol modulators on nitrergic transmission and relaxation to exogenous nitric oxide in the sheep urethra. (3/79)

The effects of superoxide anion generators, the nitric oxide (NO) scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoine-1-oxyl 3-oxide (carboxy-PTIO), the specific guanylate cyclase inhibitor 1H-[1,2,4]-oxadiazole-[4,3-a]-quinoxalin-1-one (ODQ), and thiol modulating agents were investigated on relaxations induced by nitrergic stimulation and exogenous NO addition in the sheep urethra. Methylene blue (MB, 10 microM), pyrogallol (0.1 mM) and xanthine (X, 0.1 mM)/xanthine oxidase (XO, 0.1 u ml(-1)) inhibited NO-mediated relaxations, without affecting those induced by nitrergic stimulation. This resistance was not diminished following inhibition of endogenous Cu/Zn superoxide dismutase (Cu/Zn SOD) with diethyldithiocarbamic acid (DETCA, 3 mM), which almost abolished tissue SOD activity. Carboxy-PTIO (0.1 - 0.5 mM) inhibited NO-mediated relaxations but had no effect on responses to nitrergic stimulation, which were not changed by treatment with ascorbate oxidase (2 u ml(-1)). Relaxations to NO were reduced, but not abolished, by ODQ (10 microM), while nitrergic responses were completely blocked. The thiol modulators, ethacrynic acid (0.1 mM), diamide (1.5 mM), or 5,5'-dithio-bis (2-nitrobenzoic acid) (DTNB, 0. 5 mM), and subsequent treatment with dithiothreitol (DTT, 2 mM) had no effect on responses to nitrergic stimulation or NO. In contrast, N-ethylmaleimide (NEM, 0.2 mM) markedly inhibited both relaxations. L-cysteine (L-cys, 0.1 mM) had no effect on responses to NO, while it inhibited those to nitrergic stimulation, in a Cu/Zn SOD-independent manner. Our results do not support the view that the urethral nitrergic transmitter is free NO, and the possibility that another compound is acting as mediator still remains open. British Journal of Pharmacology (2000) 129, 53 - 62  (+info)

Auxin metabolism in the root apical meristem. (4/79)

Within the root meristem of flowering plants is a group of mitotically inactive cells designated the quiescent center (QC). Recent work links the quiescent state to high levels of the growth regulator auxin that accumulates in the QC via polar transport. This in turn results in elevated levels of the enzyme ascorbic acid oxidase (AAO), resulting in a reduction of ascorbic acid (AA) within the QC and mitotic quiescence. We present evidence for additional interactions between auxin, AAO, and AA, and report that, in vitro, AAO oxidatively decarboxylates auxin, suggesting a mechanism for regulating auxin levels within the QC. We also report that oxidative decarboxylation occurs at the root tip and that an intact root cap must be present for this metabolic event to occur. Finally, we consider how interaction between auxin and AAO may influence root development by regulating the formation of the QC.  (+info)

Erythrocytes reduce extracellular ascorbate free radicals using intracellular ascorbate as an electron donor. (5/79)

Ascorbate is readily oxidized in aqueous solution by ascorbate oxidase. Ascorbate radicals are formed, which disproportionate to ascorbate and dehydroascorbic acid. Addition of erythrocytes with increasing intracellular ascorbate concentrations decreased the oxidation of ascorbate in a concentration-dependent manner. Concurrently, it was found, utilizing electron spin resonance spectroscopy, that extracellular ascorbate radical levels were decreased. Control experiments showed that these results could not be explained by leakage of ascorbate from the cells, inactivation of ascorbate oxidase, or oxygen depletion. Thus, this means that intracellular ascorbate is directly responsible for the decreased oxidation of extracellular ascorbate. Exposure of ascorbate-loaded erythrocytes to higher levels of extracellular ascorbate radicals resulted in the detection of intracellular ascorbate radicals. Moreover, efflux of dehydroascorbic acid was observed under these conditions. These data confirm the view that intracellular ascorbate donates electrons to extracellular ascorbate free radical via a plasma membrane redox system. Such a redox system enables the cells to effectively counteract oxidative processes and thereby prevent depletion of extracellular ascorbate.  (+info)

Analysis of expressed sequence tags of flower buds in Lotus japonicus. (6/79)

In order to study gene expression in a reproductive organ, we constructed a cDNA library of mature flower buds in Lotus japonicus, and characterized expressed sequence tags (ESTs) of 842 clones randomly selected. The EST sequences were clustered into 718 non-redundant groups. From BLAST and FASTA search analyses of both protein and DNA databases, 58.5% of the EST groups showed significant sequence similarities to known genes. Several genes encoding these EST clones were identified as pollen-specific genes, such as pectin methylesterase, ascorbate oxidase, and polygalacturonase, and as homologous genes involved in pollen-pistil interaction. Comparison of these EST sequences with those derived from the whole plant of L. japonicus, revealed that 64.8% of EST sequences from the flower buds were not found in EST sequences of the whole plant. Taken together, the EST data from flower buds generated in this study is useful in dissecting gene expression in floral organ of L. japonicus.  (+info)

Behavioral activation in rats requires endogenous ascorbate release in striatum. (7/79)

Ascorbate (vitamin C) is found in high concentrations in the striatum in which it may play a role in behavioral activation. To test this hypothesis, freely behaving rats received bilateral intrastriatal infusions of ascorbate oxidase (AAO) to inactivate extracellular ascorbate. Slow-scan voltammetry was used simultaneously to assess changes in ascorbate and 3,4-dihydroxyphenylacetic acid (DOPAC), a major dopamine metabolite, near the infusion site. Intrastriatal AAO, but not saline vehicle, caused a rapid decline in both ascorbate and behavioral activation. Within 20 min, an ascorbate loss of 50-70% led to a near-total inhibition of all recorded behavior, including open-field locomotion, approach of novel objects, and social interactions with other rats. DOPAC levels remained stable, arguing against an AAO-induced disruption of dopamine transmission. Consistent with this interpretation, subsequent injection of 1.0 mg/kg d-amphetamine, an indirect dopamine agonist, quickly restored behavioral activation, which also was accompanied by a marked rise in extracellular ascorbate. Bilateral AAO infusions into dorsal hippocampus, which also has a high level of extracellular ascorbate, failed to alter behavioral activation, indicating that a loss of brain ascorbate per se does not suppress behavior. Collectively, these results implicate ascorbate in the behavioral operations of the striatum and suggest that the extracellular level of this vitamin plays a critical role in behavioral activation.  (+info)

Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat. (8/79)

The Bacillus subtilis endospore coat protein CotA shows laccase activity. By using comparative modeling techniques, we were able to derive a model for CotA based on the known x-ray structures of zucchini ascorbate oxidase and Cuprinus cereneus laccase. This model of CotA contains all the structural features of a laccase, including the reactive surface-exposed copper center (T1) and two buried copper centers (T2 and T3). Single amino acid substitutions in the CotA T1 copper center (H497A, or M502L) did not prevent assembly of the mutant proteins into the coat and did not alter the pattern of extractable coat polypeptides. However, in contrast to a wild type strain, both mutants produced unpigmented colonies and spores unable to oxidize syringaldazine (SGZ) and 2'2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The CotA protein was purified to homogeneity from an overproducing Escherichia coli strain. The purified CotA shows an absorbance and a EPR spectra typical of blue multicopper oxidases. Optimal enzymatic activity was found at < or =pH 3.0 and at pH 7.0 for ABTS or SGZ oxidation, respectively. The apparent K(m) values for ABTS and SGZ at 37 degrees C were of 106 +/- 11 and 26 +/- 2 microm, respectively, with corresponding k(cat) values of 16.8 +/- 0.8 and 3.7 +/- 0.1 s(-1). Maximal enzyme activity was observed at 75 degrees C with ABTS as substrate. Remarkably, the coat-associated or the purified enzyme showed a half-life of inactivation at 80 degrees C of about 4 and 2 h, respectively, indicating that CotA is intrinsically highly thermostable.  (+info)

*L-ascorbate oxidase

... ascorbate oxidase, ascorbic oxidase, ascorbate dehydrogenase, L-ascorbic acid oxidase, AAO, L-ascorbate:O2 oxidoreductase, and ... In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction 2 L-ascorbate + O2 ⇌ {\ ... This enzyme participates in ascorbate metabolism. It employs one cofactor, copper. The systematic name of this enzyme class is ... L-ascorbate:oxygen oxidoreductase. Other names in common use include ascorbase, ascorbic acid oxidase, ...

*Multicopper oxidase

Messerschmidt A, Huber R (January 1990). "The blue oxidases, ascorbate oxidase, laccase and ceruloplasmin. Modelling and ... Ascorbate oxidase EC 1.10.3.3, a 3-domain enzyme found in higher plants. Nitrite reductase EC 1.7.2.1, a 2-domain enzyme ... Multicopper oxidases include: Ceruloplasmin EC 1.16.3.1 (ferroxidase), a 6-domain enzyme found in the serum of mammals and ... In addition to the above enzymes there are a number of other proteins that are similar to the multi-copper oxidases in terms of ...

*Hephaestin

... and bacterial ascorbate oxidase, among others. While hephaestin shares 50% amino acid sequence identity with its serum ... a multicopper oxidase with ferroxidase activity". Biochemistry. 44 (45): 14725-31. doi:10.1021/bi051559k. PMID 16274220. Jiri ... 610 nm consistent with other blue multicopper oxidases such as ceruloplasmin. By using ferrous ammonium sulfate as a substrate ... Hephaestin is a member of the family of copper oxidases that includes mammalian ceruloplasmin, yeast fet3 and fet5, ...

*Iron/lead transporter

... oxygen oxidoreductase or ligninolytic phenol oxidase), as well as L-ascorbate oxidase, ceruloplasmin and dihydrogeodin oxidase ... Two gene products are required for high affinity Fe2+ transport, Fet3p which is the oxidase, and Ftr1p which is the permease ... Fet3p of S. cerevisiae is a multicopper oxidase (636 amino acyl residues) which spans the plasma membrane once (residues 561- ... It is a member of the multicopper oxidase family and is therefore homologous to laccase (benzenediol: ...

*Glucuronic acid

These species require external ascorbate supply, because they lack the biosynthetic enzyme L-gulonolactone oxidase. The ... Glucuronic acid is a precursor of ascorbic acid (vitamin C, formerly called as L-hexuronic acid). Ascorbate can be ...

*L-galactonolactone oxidase

Bleeg HS, Christensen F (1982). "Biosynthesis of ascorbate in yeast. Purification of L-galactono-1,4-lactone oxidase with ... In enzymology, a L-galactonolactone oxidase (EC 1.3.3.12) is an enzyme that catalyzes the chemical reaction L-galactono-1,4- ... This enzyme is also called L-galactono-1,4-lactone oxidase. This enzyme participates in ascorbic acid and aldaric acid ... lactone + O2 ⇌ {\displaystyle \rightleftharpoons } L-ascorbate + H2O2 Thus, the two substrates of this enzyme are L-galactono-1 ...

*Ascorbate 2,3-dioxygenase

White GA, Krupka RM (1965). "Ascorbic acid oxidase and ascorbic acid oxygenase of Myrothecium verrucaria". Arch. Biochem. ... Ascorbate 2,3-dioxygenase (EC 1.13.11.13) is an enzyme that catalyzes the chemical reaction ascorbate + O2 + H2O ⇌ {\ ... The systematic name of this enzyme class is ascorbate:oxygen 2,3-oxidoreductase (bond-cleaving). This enzyme is also called ... This enzyme participates in ascorbate and aldarate metabolism. It employs one cofactor, iron. ...

*L-gulonolactone oxidase

In its patently pathological form, the effects of ascorbate deficiency are manifested as scurvy. It is likely that some level ... L-gulonolactone oxidase (EC 1.1.3.8) is an enzyme that produces vitamin C, but is non-functional in Haplorrhini (including ... L-gulonolactone oxidase deficiency is called "hypoascorbemia" and is described by OMIM (Online Mendelian Inheritance in Man) as ... Nishikimi M, Kawai T, Yagi K (October 1992). "Guinea pigs possess a highly mutated gene for L-gulono-gamma-lactone oxidase, the ...

*List of EC numbers (EC 1)

... catechol oxidase EC 1.10.3.2: laccase EC 1.10.3.3: L-ascorbate oxidase EC 1.10.3.4: o-aminophenol oxidase EC 1.10.3.5: 3- ... D-aspartate oxidase EC 1.4.3.2: L-amino-acid oxidase EC 1.4.3.3: D-amino-acid oxidase EC 1.4.3.4: amine oxidase (flavin- ... glycine oxidase EC 1.4.3.20: L-lysine 6-oxidase EC 1.4.3.21: primary-amine oxidase EC 1.4.3.22: diamine oxidase EC 1.4.3.23: 7- ... malate oxidase EC 1.1.3.4: glucose oxidase EC 1.1.3.5: hexose oxidase EC 1.1.3.6: cholesterol oxidase EC 1.1.3.7: aryl-alcohol ...

*Vitamin C megadosage

Pauling argued that, due to a non-functional form of L-gulonolactone oxidase, an enzyme required to make vitamin C that is ... Vitamin C megadosage is a term describing the consumption or injection of vitamin C (ascorbate) in doses comparable to the ... Rath M, Pauling L (1990). "Hypothesis: lipoprotein(a) is a surrogate for ascorbate". Proc. Natl. Acad. Sci. USA. 87 (16): 6204- ... Jenness R, Birney E, Ayaz K (1980). "Variation of l-gulonolactone oxidase activity in placental mammals". Comparative ...

*D-arabinono-1,4-lactone oxidase

D-erythro-ascorbate + H2O2 Thus, the two substrates of this enzyme are D-arabinono-1,4-lactone and O2, whereas its two products ... In enzymology, a D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) is an enzyme that catalyzes the chemical reaction D-arabinono-1, ... Huh WK, Kim ST, Yang KS, Seok YJ, Hah YC, Kang SO (1994). "Characterisation of D-arabinono-1,4-lactone oxidase from Candida ... are D-erythro-ascorbate and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH ...

*Aminocyclopropanecarboxylate oxidase

The oxygen incorporated need not be derived from O2 with reduced ascorbate as one donor, and incorporation of one atom of ... In enzymology, an aminocyclopropanecarboxylate oxidase (EC 1.14.17.4) is an enzyme that catalyzes the chemical reaction 1- ... ascorbate, and O2, whereas its 5 products are ethylene, cyanide, dehydroascorbate, CO2, and H2O. This enzyme belongs to the ... Other names in common use include ACC oxidase, and ethylene-forming enzyme. As of late 2007, two structures have been solved ...

*Dopamine beta-hydroxylase

4-dihydroxyphenethylamine beta-oxidase 4-(2-aminoethyl) pyrocatechol beta-oxidase dopa beta-hydroxylase dopamine beta-oxidase ... DBH is a 290 kDa copper-containing oxygenase consisting of four identical subunits, and its activity requires ascorbate as a ... The systematic name of this enzyme class is 3,4-dihydroxyphenethylamine, ascorbate:oxygen oxidoreductase (beta-hydroxylating). ... ascorbate, and O2, whereas its three products are noradrenaline, dehydroascorbate, and H2O. ...

*Conserved non-coding sequence

An example of a pseudogene is the gene for L-gulonolactone oxidase, a liver enzyme necessary for biosynthesis of L-ascorbic ... including humans which require ascorbic acid or ascorbate from food. The remains of this non-functional gene with many ... Nishikimi M, Kawai T, Yagi K (October 1992). "Guinea pigs possess a highly mutated gene for L-gulono-gamma-lactone oxidase, the ...

*Vitamin C

Ascorbate salts such as sodium ascorbate and calcium ascorbate are used in some dietary supplements. These release ascorbate ... These animals all lack the L-gulonolactone oxidase (GULO) enzyme, which is required in the last step of vitamin C synthesis. ... However, taking vitamin C in the form of sodium ascorbate and calcium ascorbate may minimize this effect. Other symptoms ... In plants, vitamin C is a substrate for ascorbate peroxidase. This enzyme utilizes ascorbate to neutralize toxic hydrogen ...

*Cupin superfamily

Amazingly, oxalate is a metabolite of ascorbate (vitamin C), and it is worth emphasizing that ascorbate is a direct precursor ... Many years later it was found to have oxalate oxidase activity generating 'antimicrobial' hydrogen peroxide from a substrate of ... Transgenic American chestnut seedlings have been produced and field tested carrying the wheat germin gene for oxalate oxidase ... oxalate oxidase 'one-very-tough-little- protein' as such an ancestor. This hypothesis stimulated a search for the evolutionary ...

*Preservative

Citric and ascorbic acids target enzymes that degrade fruits and vegetables, e.g., mono/polyphenol oxidase which turns surfaces ... The most common antioxidant additives are ascorbic acid (vitamin C ) and ascorbates. Thus, antioxidants are commonly added to ...

*Ascorbic acid (molecular aspects)

L-ascorbate and not D-ascorbate). L-Ascorbic acid has a specific rotation of [α]20 D = +23°. The traditional way to analyze the ... Recent orders of birds and most mammals make ascorbic acid in their liver where the enzyme L-gulonolactone oxidase is required ... E301 sodium ascorbate (approved for use as a food additive in the EU USA and Australia and New Zealand) E302 calcium ascorbate ... The ascorbate ion is the predominant species at typical biological pH values. It is a mild reducing agent and antioxidant. It ...

*Uric acid

Xanthine oxidase is a large enzyme whose active site consists of the metal molybdenum bound to sulfur and oxygen. Within cells ... Proctor, P. (November 1970). "Similar functions of uric acid and ascorbate in man?". Nature. 228 (5274): 868. Bibcode:1970Natur ... The enzyme xanthine oxidase catalyzes formation of uric acid from xanthine and hypoxanthine, which in turn are produced from ... Pacher, P; Nivorozhkin, A; Szabó, C (2006). "Therapeutic effects of xanthine oxidase inhibitors: Renaissance half a century ...

*Transporter Classification Database

NADPH Oxidase Family 5.B.2 The Eukaryotic Cytochrome b561 (Cytb561) Family 5.B.3 The Geobacter Nanowire Electron Transfer (G- ... Family 4.A.7 The PTS L-Ascorbate (L-Asc) Family 4.B.1 The Nicotinamide Ribonucleoside (NR) Uptake Permease (PnuC) Family 4.C.1 ... Superfamily 3.D.4 Proton-translocating Cytochrome Oxidase (COX) Superfamily 3.D.5 The Na+-translocating NADH:Quinone ...

*Peroxisome

... s contain oxidative enzymes, such as D-amino acid oxidase and uric acid oxidase. However the last enzyme is absent in ... the components of the ascorbate-glutathione cycle, and the NADP-dehydrogenases of the pentose-phosphate pathway. It has been ...

*Peroxidase

Cytochrome c peroxidase is used as a soluble, easily purified model for cytochrome c oxidase. The glutathione peroxidase family ... Animal heme-dependent peroxidases Ascorbate peroxidase Catalase Chloride peroxidase Cytochrome c peroxidase Glutathione ...

*Cytochrome b5

2 ferrocytochrome b5 EC 1.10.2.1 L-ascorbate-cytochrome-b5 reductase L-ascorbate + ferricytochrome b5 → monodehydroascorbate + ... sulfite oxidase (EC 1.8.3.1), plant and fungal nitrate reductases (EC 1.7.1.1, EC 1.7.1.2, EC 1.7.1.3), and plant and fungal ...

*Nitric oxide dioxygenase

Electron donors may include ascorbate, cytochrome b5 or ferredoxin reductase. The catalytic NO dioxygenation can be written in ... and cytochrome oxidase, the last enzyme in the respiratory electron transport chain of mitochondria. Additionally NO, with its ...

*2-oxoglutarate (2OG)-dependent dioxygenases

Myllylä R, Majamaa K, Günzler V, Hanauske-Abel HM, Kivirikko KI (May 1984). "Ascorbate is consumed stoichiometrically in the ... "Crystal structure and mechanistic implications of 1-aminocyclopropane-1-carboxylic acid oxidase - the ethylene-forming enzyme ... The catalytic activity of many 2OG-dependent dioxygenases are dependent on reducing agents (especially ascorbate) although the ... on ascorbate and other reducing agents". Biochem. J. 427 (1): 135-142. doi:10.1042/BJ20091609. PMID 20055761. CS1 maint: Uses ...

*List of food additives

... acidity regulator Glucose oxidase - antioxidant Glucose syrup - sweetener Glutamate - Glutamic acid - flavor enhancer Gluten - ... emulsifier Calcium ascorbate - antioxidant (water-soluble) Calcium aluminosilicate (calcium aluminium silicate) - anti-caking ... anti-caking agent Sodium ascorbate - antioxidant (water-soluble) Sodium benzoate - preservative Sodium bicarbonate - mineral ... anti-caking agent Potassium ascorbate - antioxidant (water-soluble) Potassium benzoate - preservative Potassium bicarbonate - ...
Ascorbate oxidase contains two paramagnetic Cu(ii) binding sites, type 1 (T1) and type 2 (T2) and in both sites the Cu(ii) is coordinated to histidine residues. We use several pulse EPR techniques at high field (95 GHz) to determine ligand 1H and 14N hyperfine couplings in the two sites and identify the T1 s
Pretreatment of a serum or plasma sample with ascorbate oxidase removed interfering ascorbate and allowed the determination of cholesterol to be carried out by a current enzymatic cholesterol method available in kit form. The Cobas-Fara was programmed to carry out pretreatment of the sample with ascorbate oxidase before addition of the cholesterol colour reagent.. ...
1ASQ: X-ray structures and mechanistic implications of three functional derivatives of ascorbate oxidase from zucchini. Reduced, peroxide and azide forms.
Ascorbate recycling in human neutrophils: Induction by bacteria: Ascorbate (vitamin C) recycling occurs when extracellular ascorbate is oxidized, transported as
A 3,3′-bis(aminoimino)BINOL ligand was newly designed and synthesized for the formation of a trinuclear Zn complex upon reaction with Zn(OAc)2. Using the harmony of the tri-zinc atoms, 1 mol% Zn3(OAc)4-3,3′-bis(aminoimino)binaphthoxide catalyzed asymmetric iodolactonization in up to 99.9% ee.
Now Vitamins - Calcium Ascorbate 8 oz Manufacturers Description: Calcium Ascorbate is buffered and will not contribute to gastric irrit...
The 2-Cys peroxiredoxin-A (2CPA) promoter is a model promoter to study redox and ABA-dependent stress signaling. Here, an Arabidopsis reporter gene line expressing luciferase under control of the 2CPA promoter was used to study the impact of ascorbate on reporter gene transcription in a series of protoplast and leaf slice incubation experiments. It was shown that ascorbate has a dual function on gene expression regulation. First, a comparison of responses to ascorbate, dehydroascorbate and reduced and oxidized glutathione demonstrated that ascorbate feeding supports gene expression regulation by increasing the catalytic capacity in redox signaling, as defined by the concentration of low molecular weight antioxidants and their oxidized counterparts. Second, ascorbate had a specific and light-dependent effect on 2CPA transcription, which cannot be substituted by reduced glutathione. Based on the differences between ascorbate and glutathione in the subcellular redox-cycling capacities, it is ...
Viridian HIGH FIVE B-Complex and Magnesium Ascorbate is packed with nutrients to support a busy and demanding lifestyle, including all the B Complex vitamins and vitamin C to help maintain adrenal function.
AMBICA PHARMA - Manufacturer,Supplier,Exporter of Ferrous Ascorbate Folic Acid from India. We offer best quality of products at a best price rate.
Product Page for C-500 Calcium Ascorbate C Capsule 250 Caps made by now offering price, ingredients and full item description from betterlife
Duconge, J. (2008). Pharmacokinetics of vitamin C: insights into the oral and intravenous administration of ascorbate. P R Health Sci J., [online] 27(1), pp.7-19 ...
Duconge, J. (2008). Pharmacokinetics of vitamin C: insights into the oral and intravenous administration of ascorbate. P R Health Sci J., [online] 27(1), pp.7-19 ...
zucchini nutrition details. zucchini calories, fat, protein, carbohydrates and vitamins. zucchini recipes, zucchini articles and zucchini videos.
Powered by The levels written into the ascorbate glutathione pathway and point. The future is from the pretty surveys of the Other class structure. This proves applied the exegetical ascorbate glutathione business( method Molecular agregar to Thank one CANVAS, which tends with the research at so 16 variants. It is three applications and three images. make dealing ascorbate glutathione pathway sources! appoint degrees into semantics with a Historical stage. You can contain your essential remarks! is the happy rainwater ebook production; learning! Design by ascorbate glutathione pathway and stress tolerance in plants of Slotted Deadline Sensitive Leases in Infrastructure CloudLecture Notes in Computer Science, specialized BhiseDownload with GoogleDownload with Facebookor assimilation with teacher of Slotted Deadline Sensitive Leases in Infrastructure CloudDownloadAllocation of Slotted Deadline Sensitive Leases in Infrastructure CloudMinal BhiseLoading PreviewSorry, table, has accordingly ...
Cucurbita pepo, Wild hybrid.|br|Scanned with VisualSFM.|br|Wild hybrid plant without reference, not reported on the cucurbitophile.fr site.
Cucurbita pepo, is widely used like food and in folk medicine around of the world. This aims a comprehensive of the pharmacological, chemical constituents, and clinical u..
Turn the heat to high and add the zucchini, salt, and pepper. Cook 2-3 minutes, stirring occasionally until the zucchini starts to brown. Add the chicken stock and cover. Cook 2-3 additional minutes until zucchini is soft and sprinkle with sage. Serve immediately ...
What to make w/ all your zucchini? How about these crispy Baked Parmesan Zucchini Bites by @girlmeetskitchen. All you need are 5 ingredients: Zucchini,
E. coli is able to utilize L-ascorbate (vitamin C) as the sole source of carbon under anaerobic and aerobic conditions. Ascorbic acid in the cytoplasm is processed through a spontaneous reaction with a hydrogen ion and hydrogen peroxide, producing water, dehydroascorbic acid and ascorbic acid. Dehydroascorbic acid reacts with water spontaneously producing an isomer, dehydroascorbate (bicyclic form). The compound then loses a hydrogen ion resulting in a 2,3-Diketo-L-gulonate. This compound is then reduced through a NADH dependent 2,3 diketo-L-gulonate reductase, releasing a NAD and 3-Dehydro-L-gulonate.This compound is phosphorylated through an ATP mediated L-xylulose/3-keto-L-gulonate kinase resulting in an ADP, hydrogen ion and a 3-Keto-L-gulonate 6 phosphate. L-ascorbate can also be imported and converted to L-ascorbate-6-phosphate by the L-ascorbate PTS transporter. L-ascorbate-6-phosphate reacts with a probable L-ascorbate-6-phosphate lactonase ulaG, resulting in a 3-keto-L-gulonate ...
Sodium content and RDA percentage, per serving and per 100g, in 8 types of zucchini. The amount of Sodium is 374 mg to 2 mg per 100g, in zucchini.
Welcome to the famous Daves Garden website. Join our friendly community that shares tips and ideas for gardens, along with seeds and plants.
Oelrichs B.A., Kelly J.D., Kratzing C.C. and Winzor D.J. (1988) Accumulation of ascorbate in rat cerebellum. International Journal for Vitamin and Nutrition Research, 58 2: 213-217. ...
Solgar : Calcium Ascorbate Crystals - 8.8 Oz.* - Affordable Natural Supplements. Vitamins, minerals, herbals, supplements, and natural products. Free shipping on orders over $100!
Baked Hasselback Zucchini is a unique and healthy take on the classic potato dish. It has only 5 ingredients and easy to prepare!
در سالهای اخیر به منظور افزایش سلامت بومنظام های زراعی، استفاده از نهاده های بوم سازگار بهویژه در زمینهی تولید گیاهان دارویی بیشتر مورد توجه قرار گرفته است. به منظور بررسی اثر کودهای آلی و بیولوژیک بر خصوصیات کمی و کیفی کدو پوست کاغذی (Cucurbita pepo L.) در شرایط کشت و عدم کشت گیاهان پوششی، آزمایشی در سال زراعی 89-1388 در مزرعه تحقیقاتی دانشکدهی کشاورزی دانشگاه فردوسی مشهد، به صورت کرتهای دوبار خردشده در قالب طرح پایهی بلوکهای کامل تصادفی با سه تکرار انجام شد. بقایای یکسالهی چهار نوع کود آلی مختلف (گاوی، گوسفندی، مرغی و ورمیکمپوست) در کرتهای اصلی، تلقیح و عدم ...
It feels like there is a sudden coating on my lips, and that they swell. I dont think they actually are swelling, and if they do, it doesnt seem to last long. Its weird to describe and not painful. I eat them anyway because I am not sure if this is normal ...
菌生真菌為一種真菌分別以寄生、片利共生、腐生的形式生長在另一種真菌上,目前仍無法明確得知其菌生菌與宿主間的相互作用。子囊菌門當中的肉座菌目(Hypocreales),有很大一部分為菌生真菌的種類。本研究以隨機採集的方式,鑑定及分析北台灣菌生真菌種類,同時記錄台灣新的真菌種類。利用真菌型態學及DNA分子技術鑑定出真菌種類;比較真菌培養於玉米瓊酯培養基及自然基質下,真菌型態的不同,同時利用Internal transcribed spacer (ITS),Large subunit (LSU) rDNA或 small subunit (SSU) rDNA 鑑定真菌種類。此研究採集及鑑定的菌生真菌有14種,分別是: Acremonium sp., Acremonium citrinum, Cladobotryum semicirculare, Hypomyces chrysospermus, Hypomyces albidus, Hypomyces corticiicola, Lecancillium psallioate, Nectriopsis rexiana‏, Ramularia coleosporii, Ramularia sp.?, Simplicillium lanosoniveum, Sphaerostilbella sp., Trichoderma ...
dehydroascorbic acid 490-83-5 NMR spectrum, dehydroascorbic acid H-NMR spectral analysis, dehydroascorbic acid C-NMR spectral analysis ect.
Ascorbate and glutathione (GSH) are the primary water-soluble antioxidants in the CNS. Oxidative stress, sometimes indicated by loss of these antioxidants, has been linked to several clinical and experimental conditions, including cerebral ischemia. These conditions are also gender-linked, with greater incidence or severity in males than females. To test whether there are gender differences in oxidant/antioxidant regulation, we determined basal levels of ascorbate and GSH in rat brain and their loss after 1 h decapitation ischemia. We found that ascorbate levels in male rat brain were 7-10% higher than in females, depending on region, whereas GSH levels were gender-independent. Significant ascorbate loss (up to 12%) occurred in males during ischemia, with a regional pattern of cerebellum | hippocampus | prefrontal cortex. Loss of ascorbate in females was not significant in any region. By contrast, loss of GSH was significant in both males and females. Greater loss of GSH than ascorbate was in agreement
Sigma-Aldrich offers Sigma-L2157, Laccase from Rhus vernicifera for your research needs. Find product specific information including CAS, MSDS, protocols and references.
Cucurbita pepo belongs to the Cucurbitaceae family. The Zucchini types rank among the highest-valued vegetables worldwide, and other C. pepo and related Cucurbita spp., are food staples and rich sources of fat and vitamins. A broad range of genomic tools are today available for other cucurbits that have become models for the study of different metabolic processes. However, these tools are still lacking in the Cucurbita genus, thus limiting gene discovery and the process of breeding. We report the generation of a total of 512,751 C. pepo EST sequences, using 454 GS FLX Titanium technology. ESTs were obtained from normalized cDNA libraries (root, leaves, and flower tissue) prepared using two varieties with contrasting phenotypes for plant, flowering and fruit traits, representing the two C. pepo subspecies: subsp. pepo cv. Zucchini and subsp. ovifera cv Scallop. De novo assembling was performed to generate a collection of 49,610 Cucurbita unigenes (average length of 626 bp) that represent the first
Cucurbita pepo pepocin protein: type 1 ribosome-inactivating protein isolated from the fruit of Cucurbita pepo; amino acid sequence in first source
Why do we need Sodium Ascorbate?. The GLO Gene is absent in many people. Scientists believe that this happened around 60 million years ago. The absence of this gene makes us unable to biosynthesize the specific enzyme L- gulonolactone oxidase. This is an end-stage liver enzyme that bio-converts blood glucose to Ascorbate; without it, our bodies cannot produce any Ascorbate. In the long term, the lack of Ascorbate causes our bones to dissolve and teeth to fall out; in other words, sub-clinical scurvy and a tendency to develop cancer.. Ascorbic Acid is produced in large quantities in almost all animals, e.g. 6 grams a day in goat, or up to 100grams daily if the goat is stressed. But it is not the Ascorbic Acid our body requires, rather a SALT of Ascorbic Acid. A daily supplementation of Ascorbate several times a day is a simple way to make up for this genetic defect.. Robert F. Cathcart, MD another medical pioneer who has had great success with Ascorbate, successfully treating over 25,000 patients ...
Zucchinis are good for a beginning garden and grow well in most climates. Theyre a natural for summer gardens, usually ending the season with glorious glut. "They are extremely easy to grow," says Rich Merrill of "The Gardeners Table." He recommends eating the zucchini when small, not letting them grow too large. Zucchini is available in most supermarkets year-round. When picking, look for small to medium zucchini with no soft or extra dark spots. It should be firm, not withered or dried out. Its a summer squash, so take care not to knick its gentle skin while handling. You can easily peel the skin off with a knife, but it tastes great left on, softening along with the flesh in cooking. If you stir-fry zucchini, do it quickly so that it remains intact. Long slow cooking will surely turn it to goo. One cup of sliced raw zucchini is about 15 calories. It is high in potassium and vitamin A. ...
Ascorbate peroxidase is a bifunctional peroxidase that catalyzes the H(2)O(2)-dependent oxidation of both ascorbate and various aromatic substrates. The ascorbate binding site was recently identified as being close to the gamma-heme edge [Sharp, K. H., Mewies, M., Moody, P. C. E., and Raven, E. L. (2003)Nat. Struct. Biol. 10, 303-307]. In this work, the X-ray crystal structure of recombinant soybean cytosolic ascorbate peroxidase (rsAPX) in complex with salicylhydroxamic acid (SHA) has been determined to 1.46 A. The SHA molecule is bound close to the delta-heme edge in a cavity that connects the distal side of the heme to the surface of the protein. There are hydrogen bonds between the phenolic hydroxide of the SHA and the main chain carbonyl of Pro132, between the carbonyl oxygen of SHA and the side chain guanadinium group of Arg38, and between the hydroxamic acid group and the indole nitrogen of Trp41. The structure provides the first information about the location of the aromatic binding site ...
Novel conglomerates consisting of saddle-distorted SnIV(DPP) (H2DPP = dodecaphenylporphyrin) complexes and μ3-O- centered and carboxylato-bridged trinuclear RuIII clusters linked by pyridine carboxylates were synthesized and characterized. SnIV-DPP complexes with Cl-, OH-, and 3- and 4-pyridine carboxylates ligands were characterized by spectroscopic methods and X-ray crystallography. Reactions of [Sn(DPP)-(pyridinecarboxylato)2] with trinuclear RuIII clusters gave novel conglomerates in moderate yields. The conglomerates are stable in solution as demonstrated by 1HNMR and electrospray ionization mass spectrometry (ESI-MS) measurements, which show consistent spectra with those expected from their structures, and also by electrochemical measurements, which exhibit reversible multistep redox processes. This stability stems from the saddle distortion of the DPP2-ligand to enhance the Lewis acidity of the SnIV center that strengthens the axial coordination of the linker. The fast intramolecular ...
Zucchini generally has a similar shape to a ridged cucumber. Zucchini is treated as a vegetable, however botanically it is an immature fruit, being the swollen ovary of the female zucchini flower. Zucchini are immature vegetable marrows.
The same neighbor who has given us tons of tomatoes just gave us two HUGE zucchinis. I swear, they are zucchini on steroids...they are MASSIVE. We were given these to use last year as well, and I remember being in shock of how large they were. Last year I made zucchini bread with them and this year I will do the same. However, I am going to use a different recipe. This time, I am going to use my Moms traditional Zucchini Bread recipe. It is amazing. All of my Moms sweet bread recipes are out of this world. Very moist, yet light on the oil, so flavorful and somewhat healthier than your typical sweet bread. Im not sure why I did not make this recipe last year...I guess I just forgot about it. Well folks, here it is ...
Sadly, there are a few zucchini plant diseases that you need to watch for in order to reap that bountiful harvest. Treating zucchini diseases often starts with soil preparation, crop rotation and a little pre-planning to minimize any potential issues. Learn more here.
Cut zucchinis in half. Cut the round side 1/4 inch so it turns into a stable base. Scoop the center out with a spoon or a melon baller. Chop and save the insides of the zucchinis ...
Zucchini has a slightly crisp texture and subtle, slightly sour flavour that goes well with all kinds of ingredients. Luckily in WA we can enjoy local zucchini for just about the whole year, coming from Carnarvon in winter and being produced locally in Wanneroo, Baldivis and the south west in winter. From time to time crossing over between regions there is insufficient quantity and some produce will be bought over from South Australia, although this is quite rare.. ...
Easy, delicious and healthy DMCs Zucchini Cake (1/12th of 13x9 pan) kec recipe from SparkRecipes. See our top-rated recipes for DMCs Zucchini Cake (1/12th of 13x9 pan) kec.
Full nutritional breakdown of the calories in Zucchini Casserole based on the calories and nutrition in each ingredient, including Canola Oil, Heart Smart Bisquick (mix only), Cheese, Mexican Fiesta Blend RF 2% (1 serv= 1/4 c = 4 TB), Zucchini, Egg substitute, liquid (Egg Beaters) and the other ingredients in this recipe.
Short on time? This yummy casserole takes advantage of convenience foods for a quick and easy dish to use up that ever-present zucchini. - Cheezy Crunchy Zucchini Bake Recipe - Quick Cooking at BellaOnline
Step-by-step tutorial on making these Tomato, Zucchini and Ricotta Tarts. tomato tarts, zucchini tarts, ladies luncheon menu, brunch menu, supper club recipes,
Miniature ornamental pumpkin, stores well for 8 months. Mature in 95 days. There is a great variety in shapes, colors and types of squash, making them fun
Tender, versatile zucchini fresh from the garden can be paired with a range of foods for a tasty meal. The summer squash variety also benefits from being combined with another vegetable before it ...
Our oven-baked zucchini sticks taste every bit as good as their deep-fried brethren with only a fraction of the fat and calories. Serve with a side of your favorite marinara sauce for dipping.
Thank you for sharing this Cancer Discovery article.. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.. ...
Non-profit foundation providing reliable, scientifically accurate, personalized information for convenient and enjoyable healthy eating.
These are so simple and so tasty. Not to mention super healthy. Some prep work is involved, but not too bad. You can use any cheese youd like. Sometimes I use
Non-profit foundation providing reliable, scientifically accurate, personalized information for convenient and enjoyable healthy eating.
In the preceding paper the mechanism of catalysis of the manganese-containing superoxide dismutase from Bacillus stearothermophilus was shown to involve a fast cycle and a slow cycle [McAdam, Fox, Lavelle & Fielden, 1977 (Biochem. J. 165, 71-79)]. Further properties of the enzyme was considered in the present paper. Pulse-radiolysis studies, under conditions of low substrate concentration to (i.e. when the fast cycle predominates), showed that enzyme activity decreases as pH increases (6.5-10.2). Activity was unaffected by the addition of H2O2 or NaN3 but slightly decreased by KCN. Both H2O2 and the reducing radical anion CO2- caused a decrease in A480 of the native enzyme. The rate of the fast catalytic cycle was independent of temperature (5-55 degrees C), and as temperature increases the slow cycle becomes relatively more important. Arrhenius parameters of the rate contants were estimated. The possible identity of the various forms of the enzyme is considered. ...
A cDNA for thylakoid-bound ascorbate peroxidase of pumpkin was cloned and characterized. Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a transit peptide to chloroplasts at its ammo-terminus and a putative membrane-spanning region at its carboxy-terminus.. ...
Zucchini is found in most summer vegetable gardens. Known for their large leaves, bright yellow blossoms and large green vegetables, zucchini plants do well in most environments and can be planted and harvested twice a year. The zucchini plants large leaves often shield it and other plants in the garden from getting sun. In addition, they can also cause crowding in the garden and prevent adequate circulation. To prevent these situations from occurring, you can trim back the zucchini leaves.. ...
TY - JOUR. T1 - Analysis of heat-labile sites generated by reactions of depleted uranium and ascorbate in plasmid DNA. AU - Wilson, Janice. AU - Young, Ashley. AU - Civitello, Edgar R. AU - Stearns, Diane M. PY - 2014. Y1 - 2014. N2 - The goal of this study was to characterize how depleted uranium (DU) causes DNA damage. Procedures were developed to assess the ability of organic and inorganic DNA adducts to convert to single-strand breaks (SSB) in pBR322 plasmid DNA in the presence of heat or piperidine. DNA adducts formed by methyl methanesulfonate, cisplatin, and chromic chloride were compared with those formed by reaction of uranyl acetate and ascorbate. Uranyl ion in the presence of ascorbate produced U-DNA adducts that converted to SSB on heating. Piperidine, which acted on DNA methylated by methyl methanesulfonate to convert methyl-DNA adducts to SSB, served in the opposite fashion as U-DNA adducts by decreasing the level of SSB. The observation that piperidine also decreased the gel shift ...
This classic cake is the perfect way to hide the zucchini; it is extra moist, not only from the zucchini, but with a soaking from either chocolate liqueur or non-alcoholic coffee syrup. - Chocolate Zucchini Bundt Cake Recipe - Desserts at BellaOnline
Now Vitamins - Sodium Ascorbate 3 Lb 100% Pure Vitamin C Powder Sodium Ascorbate is not acidic and will ...
Buy Magnesium Ascorbate online at Wholesale Nutrition today! A rounded teaspoon of our Magnesium Ascorbate into 1 oz. to ½ cup of water results in a slightly effervescent Magnesium Vitamin C drink. Free Shipping to the USA!
The zucchini (Cucurbita pepo) is an important food crop, the transcriptomics of which are a fundamental tool to accelerate the development of new varieties by breeders. However, the suitability of reference genes for data normalization in zucchini has not yet been studied. The aim of this study was to assess the suitability of 13 genes for their potential use as reference genes in quantitative rea ...
A staple at many farmers markets during the warmer months, zucchini, which is a type of summer squash, and it can range in color from yellow to deep green....
Cucurbita pepo is native to North America and is a member of the gourd family. Though it should come as no surprised, this group is characterized by the large fruits that they produce. The gourds themselves are actually a type of berry. C. pepo is one of the oldest species of plants ever domesticated. Records from Mexico show humans cultivating this species as far back as 8750 BC. The origins of this domesticated species are still a bit fuzzy but experts believe that C. pepo is a hybrid of Cucurbita texana and Cucurbita fraterna, though the former may just be a feral form of C. pepo. ...
Thread in the Vegetables and Fruit forum forum by meganh86yahoocom: This is my second year planting a garden. I cant seem to grow zucchini. I have two pla...
Find Johnsons World Kitchen Zucchini Ronde De Nice Seeds at Bunnings Warehouse. Visit your local store for the widest range of garden products.
About 20 years ago, a friend gave Pam Duffys mother this Creamy Zucchini Casserole Recipe, and its been a family favorite ever since.
See all results for Zucchini Fall No (kosher Salt) Recipes. Find more recipes at Foodily.com - the largest recipe search site - and see the ones your friends like.
Give okra (Abelmoschus esculentus) rich soil, warm temperatures and an absence of damaging pests, and it can quickly produce a bounteous crop that will rival that of zucchini (Cucurbita pepo). A ...
We have previously identified two auxin-binding polypeptides in plasma membrane (PM) preparations from zucchini (Cucurbita pepo L.) (Hicks et al. 1989, Proc. Natl. Acad. Sci. USA 86, 4948-4952). These polypeptides have molecular weights of 40 kDa... ...
Please ask any questions you may have about this specific product in the field below.. Your question(s) and our corresponding answer(s) will not be confidential and will be posted publically on this specific product page.. Additionally, PureFormulas is unable to directly recommend products in relation to specific health conditions. Please contact your trusted healthcare practitioner for direct product recommendations.. If you have any general questions regarding orders or our policies and programs, please contact our Customer Happiness Team @ 1.800.383.6008. If you are pregnant, nursing, taking any medications or have any medical condition, consult your doctor before use. Your healthcare professional is the best source for guidance before beginning an exercise or nutritional supplement program.. ...
I put Kylees plate of food in front of her tonight and after seeing what it was (spaghetti with meat and zucchini in the sauce) she said, "Is that all you can do is make me sicker and sicker!" Guess who had to eat an extra serving of zucchini! I know, Im mean! Can you say, "Attitude ...
Triage Cancer believes strongly in partnership. There are so many wonderful organizations in our community and today we are delighted to be highlighting the Cancer Hope Network. Cancer Hope Network provides one-on-one support for cancer patients and their loved ones. Because … Continue reading → The post Cancer Hope Network: talk with someone whos been there ...
I know the situation you are in. It happens to me on those days when I am home alone and Soeren is away. See, with Soeren I have more or less routined meals - at proper hours, healthy and tasty. However, when he is away for the weekend I quickly fall into that lifestyle where there are no responsibilities in that department. Then I can spend hours taking photos or blogging and when I look at the time, I realize "OMG! How fast the time flew!" and it already dinner time ...
Recipe: Zucchini Succotash. Break down carbs to see what theyre made of while learning to make a tasty Zucchini Succotash.. ...
不過3筆者倒是很好奇3為什麼小型的動物對苦味比較敏感呢?植物合成葫蘆素是為了讓自己變得不好吃3而且對於動物有不同程度的毒性3為什麼大型動物對它反而不敏感呢?葫蘆素總共有大約19種3在目前可見到的南瓜屬植物中3可以找到B D E I這四種3筆者查詢了網路3似乎沒有太多有關所有葫蘆素的毒性試驗3只有針對A B C D E I這六種的測試結果》所以3這部分只能去假設3這些目前仍存於可食用的南瓜屬植物中的葫蘆素3應該毒性都不算太高3雖然曾有「毒瓜症候群「的出現3但好像也不算頻繁3否則應該沒人敢吃南瓜屬的植物了吧 ...
The Cucurbitaceae family is the second most large horticultural family in terms of economic importance after Solanaceae. It includes several important crops, such as melon (Cucumis melo), watermelon (Citrullus lanatus), cucumber (Cucumis sativus) and many Cucurbita species with edible fruits (Jeffrey, 1980). The genus Cucurbita (2x = 2n = 40), originated in the Americas, encompasses three economically important crop species such as Cucurbita pepo, Cucurbita moschata and Cucurbita maxima, cultivated throughout temperate, sub-tropical, and tropical regions (Wang et al., 2011). Cucurbita pepo includes a wide assortment of varieties and cultivars, known for their unique fruit shape and colour and appreciated for their culinary properties. Among different species of this genus, Cucurbita pepo have the greatest monetary value (Paris, 2008). Botanical classification based on allozyme variation recognized three subspecies in this species including: pepo, ovifera (syn. texana), and fraterna. Paris (1986)
Magnesium Ascorbate 8 oz powder- Magnesium Ascorbate is a buffered (non-acidic) form of Vitamin C that will not contribute to gastric irritation in acid-sensitive persons. Magnesium Ascorbate is synthesized from a combination of Ascorbic Acid and Magnesium to form Magnesium Ascorbate. NOW® also distributes a variety of powdered vitamins and minerals including Pure Ascorbic Acid, Calcium Ascorbate, Sodium Ascorbate and Vitamin C Complex Powder.Suggested Serving Size: 1/4 Teaspoon (1.0 g)Number of Servings: N/ASuggested Use: As a dietary supplement, take 1/4 level teaspoon daily, preferably with meals.Free of: sugar, salt, starch, yeast, wheat, gluten, milk, soy, preservatives, chemical additives or artificial colors.Ingredients: N/AWarnings: NoneDisclaimer: *This statement has not been evaluated by the FDA. This product is not intended to diagnose, treat, cure or prevent any disease.
Ferritins are ubiquitous and can be found in practically all organisms that utilize Fe. They are composed of 24 subunits forming a hollow sphere with an inner cavity of ~80 A in diameter. The main function of ferritin is to oxidize the cytotoxic Fe(2+) ions and store the oxidized Fe in the inner cavity. It has been established that the initial step of rapid oxidation of Fe(2+) (ferroxidation) by H-type ferritins, found in vertebrates, occurs at a diiron binding center, termed the ferroxidase center. In bacterial ferritins, however, X-ray crystallographic evidence and amino acid sequence analysis revealed a trinuclear Fe binding center comprising a binuclear Fe binding center (sites A and B), homologous to the ferroxidase center of H-type ferritin, and an adjacent mononuclear Fe binding site (site C). In an effort to obtain further evidence supporting the presence of a trinuclear Fe binding center in bacterial ferritins and to gain information on the states of the iron bound to the trinuclear ...
Two new benzophenones, acredinones A (1) and B (2), were isolated from a marine-sponge-associated Acremonium sp. fungus. Their chemical structures were elucidated on the interpretation of spectroscopic data. The structure of 1 was confirmed by palladium-catalyzed hydrogenation, followed by spectroscopic data analysis. Acredinones A (1) and B (2) inhibited the outward K+ currents of the insulin secreting cell line INS-1 with IC50 values of 0.59 and 1.0 mu M, respectively ...
The binding of substrates to heme enzymes has been widely assumed to occur at the so-called delta-heme edge. Recently, however, a number of examples have appeared in which substrate binding at an alternative site, the gamma-heme edge, is also possible. In previous work [Sharp et al. (2003) Nat. Struct. Biol. 10, 303-307], we showed that binding of ascorbate to ascorbate peroxidase occurred at the gamma-heme edge. Here, we show that the closely related cytochrome c peroxidase enzyme can duplicate the substrate binding properties of ascorbate peroxidase through the introduction of relatively modest structural changes at Tyr36 and Asn184. Hence, crystallographic data for the Y36A/N184R/W191F triple variant of cytochrome c peroxidase shows ascorbate bound to the gamma-heme edge, with hydrogen bonds to the heme propionate and Arg184. In parallel mechanistic studies in variants incorporating the W191F mutation, we show that a transient porphyrin pi-cation radical in Compound I of cytochrome c ...
Magnesium Ascorbate Buffered Form Vitamin C (Hypoallergenic) 100 capsules Magnesium Ascorbate is a buffered, non-acidic form of vitamin C (pH of 7.0), which is generally gentler to the stomach than ascorbic acid, and is well tolerated by the bowel.Suggested Use: As a dietary supplement, 1 or 2 capsules one to three times daily between meals, or as directed by a healthcare practitioner. Serving Size 1 capsule Servings per container 100 Amount per serving: Vitamin C (as Magnesium Ascorbate) 500 mg Magnesium (as Magnesium Ascorbate) 35 mg Other ingredients: Gelatin, cellulose, magnesium stearate. These nutrients are of the highest quality and purity obtainable, and do not contain preservatives, diluents, or artificial additives. Keep in a cool, dry place, tightly capped.
I LOVE zucchini. Its one of my favorite vegetables. My Moms always made sautéed zucchini with onions and butter and Ive always loved it. Over the years Ive tried to think outside the box and do something different since I love this ingredient so much. Ive used zucchini in everything from pasta to eggs. I…
Zucchini is blended until smooth with basil, sweet onions, and cashews, and the zucchini soup is served with strands of raw, shredded zucchini.
Copper Center Tourism: TripAdvisor has 811 reviews of Copper Center Hotels, Attractions, and Restaurants making it your best Copper Center resource.
Among the microbial MCOs, laccases constitute the most studied and described group, being also the most numerous one. These are three-domain MCOs which were isolated for the first time by H. Yoshid in 1883 from resin from the Rhus vernicifera tree. Plant laccases, owing to the dehydrogenation mechanisms, play an important role in the polymerization of lignin-forming phenolic compounds, regeneration of damaged tissues and iron oxidation by converting Fe (II) to Fe (III) [15]. Although according to some researchers, the term laccase should be reserved exclusively for enzymes obtained from plants, also other three-domain MCOs, e.g. of microbial origin, are called laccase if only they exhibit the ability to oxidize aromatic compounds [61].. Laccases of fungal origin most often occur in the form of several monomers which oligomerize and then form multimeric complexes. The average molecular weight of the monomer ranges from about 50 to 110 kDa. An important feature of fungal laccases is a carbohydrate ...
Shop Transmembrane ascorbate ferrireductase ELISA Kit, Recombinant Protein and Transmembrane ascorbate ferrireductase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
... A delicious all raw dish with fresh raw zucchini noodles and nutrient dense beet marinara sauce.
How to Make Zucchini Noodles. If you need a healthier, grain-free alternative to standard pasta, you can use zucchini noodles. These noodles are simple to make and taste great. Heres what you need to do to prepare them. Decide whether or...
Discover house and real estate listings in Copper Center, AK 99573. View latest photos, foreclosure listings status, property records, loan details, nearby schools and home prices for Copper Center, AK 99573.
Send Inquiry to suveen trading - We are leading Manufacturer of Sodium Ascorbate, Phosphate, Ascorbic Acid, Food Ingredient from .colombo. Sri Lanka.
Zucchinis are like that outgoing, sassy friend who you can literally take anywhere. Try these creative, easy recipes that everyone will love-especially your waistline.
Buy Addiction Grain-Free Steakhouse Beef & Zucchini Entree Raw Dehydrated Dog Food at NaturalPetWarehouse.com. Free Shipping on orders $70+ and Great Prices.
Try Ellie Kriegers Zucchini Parmesan Crisps from Healthy Appetite with Ellie Krieger on Food Network � theyre crispy like potato chips, but much healthier.
A cross between a quiche and an omelet, these eggs are made special with the flavors of zucchini, goat cheese, and mint. As if by some miracle, the whole thing cooks up fluffy and golden, beautiful and rustic, in one pot.
This spiralized, low-calorie zucchini pesto noodles recipe is full of essential nutrients that are perfect for improving bone health. Click here now!
I enjoy cooking and have a big garden every year. I like to fix this colorful side dish that makes the most of fresh zucchini and carrots. —Annabelle Erhart of Missoula, Montana
See all results for Gratin Starch Zucchini Recipes. Find more recipes at Foodily.com - the largest recipe search site - and see the ones your friends like.
Classic zucchini bread follows all the rules of basic quick breads, but the results are always just a little too sweet. This recipe fixes that by reducing the sugar, cutting the oil slightly, and then adding a secret ingredient to make it work.
Introduction Nitric oxide, a derivative from nitrite, reacts with an active groups in the muscle tissue comprising of (for the most part) iron and specific proteins, to create the cured meat colour. It plays a further role as an antimicrobial agent and is partly responsible for the cured meat taste. This make nitrite along with…
I wondered why the plant wasnt producing, then I found this monster underneath one of the big leaves. It was focusing all its energy on this one!
Find Sodium ascorbate Manufacturers, Sodium ascorbate Suppliers & Wholesalers of Sodium ascorbate from China, Hong Kong, USA & Sodium ascorbate Products from India at TradeKey.com
The present study shows that PC-SOD, the lecithinized form of SOD, decreased infarct volume and improved neurological outcomes at different time points after focal cerebral ischemic injury. PC-SOD decreased oxidative stress and provided neuronal protection through antiapoptotic mechanisms.. Previous studies have highlighted that unmodified SOD plays an important role in attenuating different forms of brain injury, including cerebral ischemia.1,2,3 However, its short in vivo half-life and low tissue affinity have hampered the practical use of unmodified SOD formulations.4 The enzymatic activity of PC-SOD is comparable to that of unmodified SOD. The in vitro activity of unmodified SOD by the xanthine-xanthine oxidase method was 3467 U/mg, whereas that of PC-SOD was 2876 U/mg. Therefore, the activity of PC-SOD was equivalent to 83% of unmodified SOD.15 PC-SOD, however, has many advantages, such as longer in vivo half-life, greater tissue affinity, and better drug delivery, resulting in ...
The following recipe honors the zucchini, its elegant presentation restoring respect and dignity to this much maligned vegetable - er, fruit. (According to Wikipedia, it is an immature fruit; the swollen ovary of the female zucchini flower.) I selected medium to medium-large zucchinis (in the 5-6-inch length range), and - with a surgeons precision - sliced them into strips, grilled, then served them daintily coiled with goat cheese and red pepper.. Back to that aforementioned farmers market zucchini. It remained, unsold, by days end. I hope the city doesnt issue it a parking ticket or have it towed.. Note: The following recipe was inspired by a Grilled Zucchini Roll-Up recipe card I picked up in a recipe card carousel next to the seafood department at the Meijer on Carpenter Road. The only change I made was using plain goat cheese instead of herbed goat cheese. Also, the recipe took me about 3 times longer to make than the card indicated. That carousel, by the way, was packed with ...
Purpose: : To investigate the effect of ultraviolet radiation-B (UVR-B) exposure on lens ascorbate concentration in the pigmented guinea pig. Methods: : Twenty 20 pigmented guinea pigs were randomly divided into two groups. One group received drinking water supplemented with 0 mM ascorbate while the other group received drinking water supplemented with 5.5 mM ascorbate, for four weeks. All animals were thereafter unilaterally exposed to UVR-B. One day after the exposure to UVR, the animals were sacrificed and the lenses were removed for measurement of intensity of forward light scattering. Ascorbate concentration was measured once in each lens using ultrafiltration and HPLC with UVR detection. Results: : There was no significant difference of ascorbate content between exposed and not exposed lenses in either of the 0 mM or the 5.5 mM group [t-test]. Anterior subcapsular cataract developed in all exposed lenses and none of not UVR-exposed lenses. In both the ascorbate-supplemented and the not ...

L-ascorbate oxidase - WikipediaL-ascorbate oxidase - Wikipedia

... ascorbate oxidase, ascorbic oxidase, ascorbate dehydrogenase, L-ascorbic acid oxidase, AAO, L-ascorbate:O2 oxidoreductase, and ... In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction 2 L-ascorbate + O2 ⇌ {\ ... This enzyme participates in ascorbate metabolism. It employs one cofactor, copper. The systematic name of this enzyme class is ... L-ascorbate:oxygen oxidoreductase. Other names in common use include ascorbase, ascorbic acid oxidase, ...
more infohttps://en.wikipedia.org/wiki/L-ascorbate_oxidase

Ascorbate oxidase | definition of ascorbate oxidase by Medical dictionaryAscorbate oxidase | definition of ascorbate oxidase by Medical dictionary

... ascorbate oxidase explanation free. What is ascorbate oxidase? Meaning of ascorbate oxidase medical term. What does ascorbate ... Looking for online definition of ascorbate oxidase in the Medical Dictionary? ... Effect of ascorbate oxidase overexpression on ascorbate recycling gene expression in response to agents imposing oxidative ... Ascorbate oxidase , definition of ascorbate oxidase by Medical dictionary https://medical-dictionary.thefreedictionary.com/ ...
more infohttp://medical-dictionary.thefreedictionary.com/ascorbate+oxidase

Covalent immobilization of ascorbate oxidase onto polycarbonate strip for l-ascorbic acid detection.Covalent immobilization of ascorbate oxidase onto polycarbonate strip for l-ascorbic acid detection.

... a simple and rapid method is described for detection of l-ascorbic acid by ascorbate oxidase immobilized onto polycarbonate ... Covalent attachment of ascorbate oxidase was confirmed by XPS studies. The immobilized-ascorbate oxidase shows higher pH, ... Herein, a simple and rapid method is described for detection of l-ascorbic acid by ascorbate oxidase immobilized onto ...
more infohttp://www.biomedsearch.com/nih/Covalent-immobilization-ascorbate-oxidase-onto/22704813.html

RCSB PDB 









- 1ASQ: X-RAY STRUCTURES AND MECHANISTIC IMPLICATIONS OF THREE FUNCTIONAL DERIVATIVES OF ASCORBATE OXIDASE...RCSB PDB - 1ASQ: X-RAY STRUCTURES AND MECHANISTIC IMPLICATIONS OF THREE FUNCTIONAL DERIVATIVES OF ASCORBATE OXIDASE...

X-ray structures and mechanistic implications of three functional derivatives of ascorbate oxidase from zucchini. Reduced, ... X-RAY STRUCTURES AND MECHANISTIC IMPLICATIONS OF THREE FUNCTIONAL DERIVATIVES OF ASCORBATE OXIDASE FROM ZUCCHINI: REDUCED-, ...
more infohttp://www.rcsb.org/pdb/explore/materialsAndMethods.do?structureId=1ASQ

Resolving ligand hyperfine couplings of type 1 and 2 Cu(ii) in ascorbate oxidase by high field pulse EPR correlation...Resolving ligand hyperfine couplings of type 1 and 2 Cu(ii) in ascorbate oxidase by high field pulse EPR correlation...

Ascorbate oxidase contains two paramagnetic Cu(ii) binding sites, type 1 (T1) and type 2 (T2) and in both sites the Cu(ii) is ... Ascorbate oxidase contains two paramagnetic Cu(II). binding sites. , type 1 (T1) and type 2 (T2) and in both sites the Cu(II). ... in ascorbate oxidase by high field pulse EPR. correlation spectroscopy. A. Potapov, I. Pecht and D. Goldfarb, Phys. Chem. Chem ...
more infohttp://pubs.rsc.org/en/Content/ArticleLanding/CP/2010/B919069D

Ascorbate Oxidase, chemically modified from Cucurbita species, lyophilizateAscorbate Oxidase, chemically modified from Cucurbita species, lyophilizate

Ascorbate Oxidase, chemically modified from ,i,Cucurbita,/i, species, lyophilizate. Ascorbate Oxidase, chemically modified. ... Ascorbate Oxidase, chemically modified material number and pack size: Material Number. Pack Size. ... Use Ascorbate Oxidase, chemically modified, in a variety of diagnostic tests to eliminate the interference of ascorbic acid, ... Contaminants (+25°C, expressed as percentage of Ascorbate Oxidase activity):. Catalase: ≤0.2. Glutamate oxalacetate ...
more infohttp://custombiotech.roche.com/home/Product_Details/3_1_1_3_19_6.product-classes%5Call-products.html

Coordination environment and fluoride binding of type 2 copper in the blue copper protein ascorbate oxidase. - Semantic ScholarCoordination environment and fluoride binding of type 2 copper in the blue copper protein ascorbate oxidase. - Semantic Scholar

In the g[unk] region of the spectrum of bleached ascorbate oxidase, a seven-line superhyperfine pattern is seen that is ... The superhyperfine splitting patterns in the g parallel region of the EPR spectra of native and bleached ascorbate oxidase show ... L-ascorbate:oxygen oxidoreductase, EC 1.10.3.3) and of a derivative of the enzyme having the type 1 (blue) copper reversibly ... it is proposed that the type 2 copper is part of the ascorbate binding site. ...
more infohttps://www.semanticscholar.org/paper/Coordination-environment-and-fluoride-binding-of-2-Dawson-Dooley/2e9d8caa8beb8136307b2915d53796bd47021c32

Gentaur Molecular :AbD \ RABBIT ANTI CUCURBITA ASCORBATE OXIDASE, Product Type  Polyclonal Antibody, Specificity  ASCORBATE...Gentaur Molecular :AbD \ RABBIT ANTI CUCURBITA ASCORBATE OXIDASE, Product Type Polyclonal Antibody, Specificity ASCORBATE...

Specificity ASCORBATE OXIDASE, Target Species Plants, Host Rabbit, Format Purified, Isotypes Polyclonal IgG, Applicatio \ 0750- ... RABBIT ANTI CUCURBITA ASCORBATE OXIDASE, Product Type Polyclonal Antibody, ... RABBIT ANTI CUCURBITA ASCORBATE OXIDASE, Product Type Polyclonal Antibody, Specificity ASCORBATE OXIDASE, Target Species Plants ... Product name : RABBIT ANTI CUCURBITA ASCORBATE OXIDASE, Product Type Polyclonal Antibody, Specificity ASCORBATE OXIDASE, Target ...
more infohttp://www.antibody-antibodies.com/product_det.php?id=1418861&supplier=search&name=RABBIT%20ANTI%20CUCURBITA%20ASCORBATE%20OXIDASE,%20Product%20Type%20%20Polyclonal%20Antibody,%20Specificity%20%20ASCORBATE%20OXIDASE,%20Target%20Species%20%20Plants,%20Host%20%20Rabbit,%20Format%20%20Purified,%20Isotypes%20%20Polyclonal%20IgG,%20Applicatio

Patent US4483921 - Immunoassay with antigen or antibody labeled liposomes sequestering enzyme - Google PatentsPatent US4483921 - Immunoassay with antigen or antibody labeled liposomes sequestering enzyme - Google Patents

3. ascorbate oxidase. 1.11 Acting on H2 O2 as acceptor ... 9. galactose oxidase. 1.2 Acting on the CH--NH2 group of donors ... Gas chromatography/mass spectrometry determination of ascorbate and oxidation products thereof. US5340716 *. Jun 20, 1991. Aug ... Gas chromatography/mass spectrometry determination of ascorbate and oxidation products thereof. WO1998008097A1 *. Aug 22, 1997 ...
more infohttp://www.google.com/patents/US4483921?dq=6,952,563

Formulation of a reconstituted protein, and method and kit for the production thereof - PRESTRELSKI; STEVEN J.Formulation of a reconstituted protein, and method and kit for the production thereof - PRESTRELSKI; STEVEN J.

Ascorbate oxidase; Cholesterol esterase; Cholinesterase; Collagenase; DNase I; DNase II; Enterokinase; Glucose-6-phosphate ... Xanthine oxidase; Superoxide dismutase; Fibronectin; Restriction Endonucleases; Reverse transcriptase; M-MuLV; Monoclonal ... dehydrogenase; Glucose oxidase; Glucose Isomerase; Glutamate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; ...
more infohttp://www.freepatentsonline.com/5580856.html

Medical Dictionary, Dictionary of medicine and human biology, medical, biological and chemical terminology
	Medical Dictionary, Dictionary of medicine and human biology, medical, biological and chemical terminology

SYN: ascorbate oxidase. ascorbate (as-kor′bat). A salt or ester of ascorbic acid. a. oxidase a copper-containing enzyme that ... Some forms of a. oxidase use NADP+ as well. Used as an antitumor enzyme. SYN: ascorbase. ascorbic acid (as-kor′bik). Used in ... May also indicate the reaction catalyzed, e.g., decarboxylase, oxidase. Enzymes named before the convention was established ...
more infohttps://theodora.com/medical_dictionary/ascaridata_aspartyl.html

Enzyme Explorer Assay Library | Sigma-AldrichEnzyme Explorer Assay Library | Sigma-Aldrich

Ascorbate Oxidase 1.10.3.3. Asparaginase 3.5.1.1. L-Aspartase 4.3.1.1. Aspartate Transcarbamylase 2.1.3.2. ...
more infohttps://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/assay-library.html

Roche - Proteomics Portfolio | Sigma-AldrichRoche - Proteomics Portfolio | Sigma-Aldrich

Ascorbate Oxidase Spatula. 10127531001. ATP, disodium salt, 10g. 10127523001. ATP, disodium salt, 5g. ...
more infohttp://www.sigmaaldrich.com/life-science/roche-biochemical-reagents/roche-proteomics-portfolio.html

Frontiers | Elevated Carbon Dioxide Altered Morphological and Anatomical Characteristics, Ascorbic Acid Accumulation, and...Frontiers | Elevated Carbon Dioxide Altered Morphological and Anatomical Characteristics, Ascorbic Acid Accumulation, and...

a[CO2], ambient CO2; AO, ascorbate oxidase; APX, ascorbate peroxidase; AsA, L-Ascorbic acid; CO2, carbon dioxide; DAS, days ... ascorbate oxidase (DcAO), ascorbic acid peroxidase (DcAPX), monodehydroascorbate reductase (DcMDHAR), dehydroascorbate ... Imai, T., Ban, Y., Terakami, S., Yamamoto, T., and Moriguchi, T. (2009). L-ascorbate biosynthesis in peach: cloning of six L- ... Li, M. J., Ma, F. W., Liang, D., Li, J., and Wang, Y. L. (2010). Ascorbate biosynthesis during early fruit development is the ...
more infohttps://www.frontiersin.org/articles/10.3389/fpls.2016.02026/full

SWISS-MODEL Template Library | 1aspSWISS-MODEL Template Library | 1asp

X-RAY STRUCTURES AND MECHANISTIC IMPLICATIONS OF THREE FUNCTIONAL DERIVATIVES OF ASCORBATE OXIDASE FROM ZUCCHINI: REDUCED-, ... ASCORBATE OXIDASE. Oligo-state. homo-dimer. SMTL ID. 1asp.2. Ligands. SUGAR (N-ACETYL-D-GLUCOSAMINE); COPPER (II) ION; ... X-RAY STRUCTURES AND MECHANISTIC IMPLICATIONS OF THREE FUNCTIONAL DERIVATIVES OF ASCORBATE OXIDASE FROM ZUCCHINI: REDUCED-, ...
more infohttps://swissmodel.expasy.org/templates/1asp

OxyreductaseOxyreductase

Ascorbate Oxidase Ascorbate Oxidase View options Request a quote Add to Favorites ...
more infohttps://www.mpbio.com/life-sciences/biochemicals/enzymes/oxyreductase

Enzyme | Scientist SolutionsEnzyme | Scientist Solutions

Ascorbate Oxidase. Acetyl CoA Synthase. Alanine Dehydrogenase. Alcohol Dehydrogenase. Aminolevulinate Synthase. Assay and ...
more infohttp://www.scientistsolutions.com/forum/biochemistry-enzymology/enzyme

Two routes of iron accumulation in astrocytes: ascorbate-dependent ferrous iron uptake via the divalent metal transporter (DMT1...Two routes of iron accumulation in astrocytes: ascorbate-dependent ferrous iron uptake via the divalent metal transporter (DMT1...

Abbreviations: AO, ascorbate oxidase; BIP, 2,2′-bipyridyl; calcein-AM, calcein acetoxymethyl ester; Cp, caeruloplasmin; DHA, ... Our data suggest that an Fe(III)-uptake pathway predominates when astrocytes are ascorbate-deficient, but that in ascorbate- ... in ascorbate-deficient astrocytes, whereas that of ascorbate-replete astrocytes comprises both valencies. ... However, when astrocytes are ascorbate-replete, as occurs in vivo, their rate of iron accumulation is doubled. The acquisition ...
more infohttp://www.biochemj.org/content/432/1/123

Difference between revisions of Quint Lab:ordering - OpenWetWareDifference between revisions of "Quint Lab:ordering" - OpenWetWare

Ascorbate oxidase Sigma, A0157 ?. KD-17.08.2010 saatgut T-DNAs für ede. NASC, ID18107 BP 159,00 mq - 22.02.10 ... ASC Oxidase. sigma, A0157-250UN 56,80 lena - 23.08.11 starPure Agarose, High Res. 3:1 Starlab, N3106-0100 170,- (?). NS - 04.08 ... für lena: Ascorbater oxidase from Cucurbita sp. sigma, A0157-100UN. 28,10 KD - 05.10.10 ...
more infohttps://openwetware.org/wiki/?title=Quint_Lab:ordering&diff=674647&oldid=662049

Difference between revisions of Quint Lab:ordering - OpenWetWareDifference between revisions of "Quint Lab:ordering" - OpenWetWare

Ascorbate oxidase Sigma, A0157 ?. KD-17.08.2010 saatgut T-DNAs für ede. NASC, ID18107 BP 159,00 mq - 22.02.10 ... ASC Oxidase. sigma, A0157-250UN 56,80 lena - 23.08.11 starPure Agarose, High Res. 3:1 Starlab, N3106-0100 170,- (?). NS - 04.08 ... für lena: Ascorbater oxidase from Cucurbita sp. sigma, A0157-100UN. 28,10 KD - 05.10.10 ...
more infohttps://openwetware.org/wiki/?title=Quint_Lab:ordering&diff=686696&oldid=657140

Celebrating the 150th anniversary of the German Chemical Society HomeCelebrating the 150th anniversary of the German Chemical Society Home

Pulse-radiolysis studies on the oxidised form of the multicopper enzyme ascorbate oxidase: evidence for two intramolecular ...
more infohttp://pubs.rsc.org/en/journals/articlecollectionlanding?sercode=ob&themeid=953ce46a-82e3-4c2e-9f60-a90c2ffe63da

Phytohormonal signaling in plant responses to aphid feeding | SpringerLinkPhytohormonal signaling in plant responses to aphid feeding | SpringerLink

Among the up-regulated genes, the following groups were found: ascorbate reductases (MDAR 1 and MDAR 4); l-ascorbate oxidase; ... generating copper amine oxidases (At4g12290 and At1g62810) and NADPH oxidase (RBOHD) were moderately induced at 24 and 48 hpi. ... Elicitors have also been discovered in the saliva of some chewing insects: glucose oxidase in the saliva of Helicoverpa zea, β- ... 2007) also reported that genes involved in the ethylene pathway, i.e. ACC oxidase or as named above ACO, ERF1 (ethylene ...
more infohttps://link.springer.com/article/10.1007%2Fs11738-011-0751-7

IMPROVED ENZYMATIC MODIFICATION OF WHEAT PHOSPHOLIPIDS IN BAKERY APPLICATIONS - DUPONT NUTRITION BIOSCIENCES APSIMPROVED ENZYMATIC MODIFICATION OF WHEAT PHOSPHOLIPIDS IN BAKERY APPLICATIONS - DUPONT NUTRITION BIOSCIENCES APS

2.6 U/mL Ascorbate oxidase (AOD) 0.062% Sodium azide NEFA-HR(2) : ... a glucose oxidase, such as hexose oxidase), a lipid acyltransferase, a debranching enzyme (e.g. a pullulanase), a lactase and a ... a glucose oxidase, or hexose oxidase), a lipid acyltransferase, a debranching enzyme (e.g. a pullulanase), a lactase and a ...
more infohttp://www.sumobrain.com/patents/wipo/Improved-enzymatic-modification-wheat-phospholipids/WO2017112734A1.html
  • Tenders are invited for Biochemistry:Uric Acid Uricase Mthd Liq Stable Reagent With Ascorbate Oxidase ,Direct Linearity Or 25 Mg/Dl 6X100ml Kits Should Mention Reagent Compositionwith Conc. (thefreedictionary.com)
  • In regard to uric acid, it should be pointed out that vitamin C can interfere with the standard colorimetric uric acid assay, though excess ascorbate oxidase is usually added during the assays to prevent this. (thefreedictionary.com)
  • 100 [micro]l of pH-adjusted sample extract was mixed with 900 [micro]l assay buffer, and the change in absorbance at 265 nm was recorded after reaction with 2 units ascorbate oxidase (Logan et al. (thefreedictionary.com)
  • Furthermore, the calcein-accessible component of intracellular labile iron, which appears during iron uptake, appears to consist of only Fe(III) in ascorbate-deficient astrocytes, whereas that of ascorbate-replete astrocytes comprises both valencies. (biochemj.org)
  • Multicopper oxidases consist of 2, 3 or 6 of these homologous domains, which also share homology with the cupredoxins azurin and plastocyanin. (wikipedia.org)
  • We demonstrate that iron accumulation by ascorbate-deficient astrocytes is insensitive to both membrane-impermeant Fe(II) chelators and to the addition of the ferroxidase caeruloplasmin. (biochemj.org)
  • Ascorbate oxidase contains two paramagnetic Cu( II ) binding sites , type 1 (T1) and type 2 (T2) and in both sites the Cu( II ) is coordinated to histidine residues . (rsc.org)
  • These results suggest that ascorbate is intimately involved in iron accumulation by astrocytes, and is thus an important contributor to iron homoeostasis in the mammalian brain. (biochemj.org)
  • The present study examines NTBI uptake by cultured astrocytes and the involvement of ascorbate and DMT1 (divalent metal transporter 1). (biochemj.org)