Development of a sensitive, specific reverse transcriptase polymerase chain reaction-based assay for epithelial tumour cells in effusions. (1/1795)

We developed a sensitive and specific method for the detection of epithelial cancer cells in effusions with a two-stage molecular-based assay which combined enrichment for cancer cells by immunomagnetic bead selection and reverse transcriptase polymerase chain reaction (RT-PCR) detection of epithelial glycoprotein 2 (EGP-2) RNA. Preliminary experiments indicated that immunobead selection was essential to avoid occasional false-positive RT-PCR results, and this method detected ten breast cancer cells electively added to 10(7) cytologically negative effusion cells. We studied 110 cases of pleural (n = 68) and peritoneal (n = 42) effusions (30 from patients with known carcinoma and 80 from those without known carcinoma), and the results were compared with cytological findings. Of 18 effusions that were cytologically positive or suspicious for malignant cells, 17 (94%) were positive for EGP-2 RNA (the one negative sample was from a patient who recently received combination chemotherapy). Of 92 cytologically negative samples, 11 (12%) were positive for EGP-2, including six patients with a history of previous or current carcinoma. Our method appears to be highly specific and increases the sensitivity of detection of malignant cells; it may be a useful adjunct to routine cytopathological examination.  (+info)

Peritoneal cytology in the surgical evaluation of gastric carcinoma. (2/1795)

Many patients undergoing surgery for gastric carcinoma will develop peritoneal metastases. A method to identify those patients at risk of peritoneal recurrence would help in the selection of patients for adjuvant therapy. Peritoneal cytology has received little attention in the West, but may prove a useful additional means of evaluating patients with gastric cancer. The aims of this study were to evaluate sampling techniques for peritoneal cytology in patients with gastric cancer, to assess the prognostic significance of free peritoneal malignant cells and to discover the effect of the operative procedure on dissemination of malignant cells. The study is based on 85 consecutive patients undergoing surgical treatment of gastric cancer and followed up for 2 years or until death. Peritoneal cytology samples were collected at laparoscopy, and at operation prior to resection by intraperitoneal lavage and serosal brushings. After resection, samples were taken by peritoneal lavage, imprint cytology of the resected specimen and post-operatively by peritoneal irrigation via a percutaneous catheter. Malignant cells were diagnosed by two independent microscopists. Preoperative peritoneal lavage yielded malignant cells in 16 out of 85 cases (19%). The yield of free malignant cells was increased by using serosal brushings (by four cases) and imprint cytology (by two cases); all of the cases had evidence of serosal penetration. One serosa-negative case exhibited positive cytology in the post-resection peritoneal specimen in which the preresection cytology specimen was negative. Survival was worse in the cytology-positive group (chi2 = 25.1; P< 0.0001). Among serosa-positive patients, survival was significantly reduced if cytology was positive, if cases yielded by brushings and imprint cytology were included (log-rank test = 8.44; 1 df, P = 0.004). In conclusion, free peritoneal malignant cells can be identified in patients with gastric cancer who have a poor prognosis; the yield can be increased with brushings and imprint cytology in addition to conventional peritoneal lavage. Evaluation of peritoneal cytology by these methods may have a role in the selection of patients with the poorest prognosis who may benefit most from adjuvant therapy.  (+info)

Immunocytochemically detected free peritoneal tumour cells (FPTC) are a strong prognostic factor in gastric carcinoma. (3/1795)

We prospectively investigated the prognostic significance of free peritoneal tumour cells (FPTC) in a series of 118 patients with completely resected gastric carcinoma. Immunocytochemistry with the monoclonal antibody Ber-Ep4 was performed on cytospins from intraoperative peritoneal lavage specimens. Twenty-three patients (20%) had FPTC which was significantly correlated with pT and pN categories, stage, tumour size, lymphatic invasion, Lauren and WHO classifications and perigastric adipose tissue metastases. The median survival time for all FPTC positive compared with negative patients was significantly shorter (11 compared with >72 months), with estimated 5-year survival rates of 8% vs. 60%. None of the patients with FPTC had an early gastric cancer. In advanced tumour subgroups without and with serosal invasion (n = 59 and 35), there were 19% and 34% with FPTC. Multivariate survival analysis showed nodal status, FPTC, mesenteric lymphangiosis, and lymph node metastasis to the compartment III to be independent prognostic factors with relative risks of 6.6, 4.5, 2.9 and 2.2 respectively. Recurrent disease occurred in 91% of FPTC-positive and in 38% of FPTC-negative patients. FPTC had a positive predictive value of 91% and a specificity of 97% for tumour recurrence. FPTC is a strong negative, independent prognostic indicator for survival in gastric carcinoma.  (+info)

Paracrine changes in the peritoneal environment of women with endometriosis. (4/1795)

During the past decade, macrophage-derived substances such as prostanoids, cytokines, growth factors and angiogenic factors have been detected in the peritoneal fluid of women with endometriosis. In particular, growth-promoting and angiogenic factors are considered to be substantially involved in the pathogenesis of endometriosis. In this study, vascular endothelial growth factor (VEGF), transforming growth factor beta (TGF-beta) and intercellular adhesion molecule 1 (ICAM-1), substances recently detected in the peritoneal fluid of women with endometriosis, were assessed with regard to their concentrations in different stages of endometriosis and changes of the peritoneal paracrine activity after medical treatment with a gonadotrophin releasing hormone agonist (GnRHa). Peritoneal fluid was obtained from patients with endometriosis during laparoscopy before and after a 4-month treatment with a GnRHa. VEGF, TGF-beta and ICAM-1 could be detected in all women presenting with various stages of active endometriosis. After GnRHa therapy, all patients showed significant decreases in mean concentrations of VEGF (194+/-77 pg/ml), TGF-beta (902+/-273 pg/ml) and ICAM-1 (157+/-52 ng/ml). Patients with stage III and IV endometriosis (according to the rAFS score) had much higher concentrations of VEGF and TGF-beta before treatment compared with those patients with mild endometriosis (rAFS stages I and II). The most striking decrease in concentration was for TGF-beta, from 902 pg/ml before to 273 pg/ml after therapy. These results indicate an important role for paracrine activity in the establishment and maintenance of endometriosis. Indeed, treatment with a GnRHa may reduce paracrine activity in the peritoneal cavity via hypo-oestrogenism and provide proof of successful therapy.  (+info)

Tracing cellular and molecular mechanisms involved in endometriosis. (5/1795)

The aetiology and pathogenesis of endometriosis, defined as the presence of endometrium-like tissue outside the uterine cavity, is largely unknown. In this paper we present and discuss possibilities to study the putative pathogenic properties of endometriotic cells in vitro. The current focus of our investigations is on the invasive phenotype of the disease, assuming that this might contribute to the pathogenesis of endometriosis. So far, we have shown that: (i) cytokeratin-positive and E-cadherin-negative endometriotic cells have an invasive phenotype in a collagen invasion assay in vitro similar to metastatic carcinoma cells; (ii) the invasiveness of endometriotic but not of eutopic endometrial cells can be stimulated by a heat-stable protein present in peritoneal fluid; and (iii) the endometriotic cell line EEC145T, which we established, may be a useful tool for the identification of gene products which are, positively or negatively, invasion-related. Finally, our studies suggest that the invasive phenotype in endometriosis shares aspects with tumour metastasis, but might also have unique mechanisms.  (+info)

Endometriotic disease: the role of peritoneal fluid. (6/1795)

Peritoneal fluid and the intraovarian milieu are a specific microenvironment. Peritoneal fluid originates mainly as an ovarian exudation product caused by increased vascular permeability, with cyclic variation in volume and steroid hormones which are always higher than in plasma. It contains large amounts of macrophages and their secretion products, and has a large exchange area with plasma through the peritoneum, which is highly permeable for small molecules. Diffusion becomes virtually zero for molecules with a molecular weight of >100000 Da. In women with the luteinized unruptured follicle (LUF) syndrome, concentrations of oestrogens and progesterone are much lower in the luteal phase. Endometriosis is associated with sterile low-grade inflammation, increased concentrations of activated macrophages and many of their secretions, such as cytokines, growth factors and angiogenic factors. Concentrations of CA-125 and of glycodelins are also increased, secreted locally by the endometrial cells. Natural killer (NK) cell function declines, possibly mediated by glycodelins or local intercellular adhesion molecule (ICAM) -1 shedding. The ovary is also a specific microenvironment, with steroid hormone concentrations 1000-fold higher in follicles than in plasma. Endometrial and superficially implanted cells are influenced by peritoneal fluid concentrations so that local environment, rather than inherent cellular differences could explain differences between superficial endometriosis and eutopic endometrium. Differences between superficial implants and endometriotic disease, deep infiltrating or cystic ovarian endometriosis, may thus arise via different endocrine environments. Superficial endometrial implants are regulated by peritoneal fluid factors, whereas deep endometriosis and cystic ovarian endometriosis are influenced by blood or ovarian factors. The endometriotic disease theory considers superficial endometriotic implants and their remodelling as a physiological process in most women, and concentrates on the causes of severe endometriosis such as differences in the eutopic endometrium from women with and without endometriosis (which may indicate hereditary differences), the invasiveness of some endometriotic cells in vitro, focal 'shielding' of endometriotic foci by adhesions, and inhibition of NK activity by ICAM-1 and glycodelins. Endometriotic disease is thus seen as a benign tumour. The type of cellular lesion, hereditary and immunological environments and local hormone concentrations in the ovary and in peritoneal fluid, will decide expression as cystic ovarian endometriosis, deep endometriosis or adenomyosis externa, and whether the latter is associated with adhesions.  (+info)

2-Deoxyglucose selectively inhibits Fc and complement receptor-mediated phagocytosis in mouse peritoneal macrophages II. Dissociation of the inhibitory effects of 2-deoxyglucose on phagocytosis and ATP generation. (7/1795)

Macrophages incubated in 2-deoxy-D-glucose (2-dG)-containing medium showed a marked decrease in cellular ATP content, and were unable to ingest IgG- and complement-coated erythrocytes via the corresponding membrane receptors for these ligands. However, the inhibitory effects of 2-dG on Fc- and C3 receptor-mediated phagocytosis were not a consequence of lowered macrophage ATP levels since addition of glucose or mannose to the culture medium restored the capacity of the macrophages to ingest IgG- and C3-coated particles without increasing ATP levels. These results indicate that Fc- and C3 receptor-mediated phagocytosis (opsonin dependent) differs qualitatively from the ingestion of latex and zymosan particles (opsonin independent); they suggest that the same regulatory molecules govern the responses of phagocytic cells to signals initiated by both the Fc and C3 receptors. The possibility that these molecules are regulated by glycosylation is discussed.  (+info)

The requirement of an adherent cell substratum for the growth of developing plasmacytoma cells in vivo. (8/1795)

The intraperitoneal injection of pristane (2,6,10,14-tetramethylpentadecane) produces an environment conductive to primary plasmacytoma growth in as few as 3 days. After pristane injection, the total free peritoneal cell population increases from a normal value of 1.55 X 10(6) to 5.28 X 10(6) and remains at this elevated level for at least 50 days. The adherent peritoneal cell population, composed of both mononuclear cells and polymorphonuclear leukocytes, is the primary source of this increase. In the pristane-conditioned peritoneum, these cells rapidly form a chronic granuloma on the peritoneal connective tissues. Daily subcutaneous treatment of mice with 0.5 mg of hydrocortisone beginning simultaneously with pristane injection prevents the increase in the peritoneal cell population, granuloma formation, d the production of a conditoned environment. In mice treated with hydrocortisone beginning 3 days after pristane injection, however, neither the peritoneal cell increase nor the production of a conditioned environment is prevented. The intraperitoneal injection of thioglycolate medium at 4-day intervals produces an elevation of the free adherent peritoneal cell population similar to pristane, but does not produce a granuloma or a conditioned environment. The intraperitoneal transfer of thioglycolate-induced adherent peritonel cells to mice treated with pristane and hydrocortisone simultaneously restores the production of a conditioned environment. These findings indicate that the adherent peritoneal cell population is responsible for the conditioning effect, and that the establishment of a resident population of these cells is necessary to produce conditioning.  (+info)

Pleural or ascitic fluid should be sent for analysis. An elevated amylase level, usually > 1,000 IU/L, with protein levels over ... Loss of a small volume of fluid will not cause a problem but an acidosis is common if the volume of pancreatic fluid lost from ... Loss of bicarbonate-rich pancreatic fluid via a pancreatic fistula can result in a hyperchloraemic or normal anion gap ... ISBN 0-7216-2082-5 Dugernier T, Laterre PF, Reynaert MS (2000). "Ascites fluid in severe acute pancreatitis: from ...
... ascitic) fluid. The needle is removed, leaving the plastic sheath to allow drainage of the fluid. The fluid is drained by ... The color of the ascitic fluid can also be useful in analysis. Blood fluid can indicate trauma or malignancy. A milky ... A z-track is a technique that allows for decreased ascitic fluid leak following the paracentesis by displacing the needle ... Straw or light yellow colored fluid indicates more plasma-like and benign causes of peritoneal ascites. The ascitic white blood ...
Runyon BA (1986). "Low-protein-concentration ascitic fluid is predisposed to spontaneous bacterial peritonitis". ... Norfloxacin is used for prevention of spontaneous bacterial peritonitis in cirrhotic patients who have a low ascites fluid ...
Diagnostic value in ascitic fluid and serum". Tuberculosis. 71 (2): 121-126. doi:10.1016/0041-3879(90)90007-U. Apollo Hospital ...
This phenomenon is the result of Starling's forces between the fluid of the circulatory system and ascitic fluid. Under normal ... The formula is as follows: SAAG = (serum albumin) − (albumin level of ascitic fluid). Ideally, the two values should be ... The SAAG may be a better discriminant than the older method of classifying ascites fluid as a transudate versus exudate. ... The increase in hydrostatic pressure causes more fluid to leave the circulation into the peritoneal space (ascites). The SAAG ...
"Cytology of ascitic fluid in a patient with metastasizing malignant Brenner tumor of the ovary. A case report". Acta Cytologica ... with the fluid-filled spaces Call-Exner bodies between the granulosa cells. Transitional cell carcinoma is an even rarer entity ...
Maeda H, Matsumura Y, Kato H (Nov 1988). "Purification and identification of [hydroxyprolyl3]bradykinin in ascitic fluid from a ...
SAAG is calculated by subtracting the albumin measure of ascitic fluid from the serum value. In portal hypertension, the SAAG ... Peritoneal fluid is a serous fluid made by the peritoneum in the abdominal cavity which lubricates the surface of tissue that ... An increased volume of peritoneal fluid is called ascites. Sampling of peritoneal fluid is generally performed by paracentesis ... Peritoneal fluid microscopy is a useful test in evaluating the cause of ascites. A diagnostic peritoneal lavage (DPL) is ...
"Cytology of ascitic fluid in a patient with metastasizing malignant Brenner tumor of the ovary. A case report". Acta Cytologica ... Call-Exner bodies, giving a follicle-like appearance, are small eosinophilic fluid-filled punched out spaces between granulosa ...
It is specifically an infection of the ascitic fluid - an increased volume of peritoneal fluid. Ascites is most commonly a ... Diagnosis is made by paracentesis (needle aspiration of the ascitic fluid); SBP is diagnosed if the fluid contains neutrophils ... As for the significance of ascitic fluid proteins, it was demonstrated that cirrhotic patients with ascitic protein ... Additional studies have confirmed the validity of the ascitic fluid protein concentration as the best predictor of the first ...
Ahr, A.; Arnold, G.; Göhring, U. J.; Costa, S.; Scharl, A.; Gauwerky, J. F. (July 1997). "Cytology of ascitic fluid in a ... with the fluid-filled spaces Call-Exner bodies between the granulosa cells.[3] They are large enough to be seen in an optical ...
Pleural fluid can far more easily cause symptoms than ascitic fluid, due to the lower volume of the pleural cavity as compared ... The fluid is a transudate and similar to fluid found in ascites. There may be a higher protein and albumin content in hepatic ... Fluid moves from the abdomen to the pleural cavity via a difference in pressure between the cavities. If the rate of fluid ... After this, the fluid is analyzed for diagnosis and to help rule out other possible causes like infection or cancer. The fluid ...
... we should spend this time not divining for ascitic fluid (ultrasound is better) but, instead, talking to our patients. His ...
... then the air-fluid level would not move. Shifting dullness is usually present if the volume of ascitic fluid is up to 500 mL ml ... or has less than 2 litres of free fluid present. If the fluid causing the dullness was not free, ... After waiting sufficient time for any fluid to shift (up to 30 seconds), the dull position is then percussed. It may now be ... Abdominal examination Fluid wave test Bulging flanks Puddle sign Murray Longmore, Ian B. Wilinson, Edward H. Dawvidson, ...
The ascitic fluid of the organism can also contain neoplastic cells that may or may not be malignant and cancerous. The disease ... Fluid begins to build up in the abdomen of the infected organism, causing large ascites. Once the viral genome is incorporated ...
It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It ...
Primary ovarian adenosarcoma with elevated Ca-125 levels and normal ascitic fluid cytology: a case report and review of ...
Soluble HLA-G can be found in the saliva, ascitic fluid, plasma, thymus, seminal plasma, cerebrospinal fluid, and in first and ...
L1210 is a mouse lymphocytic leukemia cell line which is derived from the ascitic fluid of 8-month-old female DBA/2 strain mice ...
Trypticase soy agar or broth enriched with 20% blood, serum, or ascitic fluid is necessary for the optimal growth of the ...
... common radiographic feature among patients with suspected tuberculous peritonitis was septated compartments of ascitic fluid on ... Vardareli, E; Kebapcı, M; Saricam, T; Pasaoglu, Ö; Açikalin, M (2004-03-01). "Tuberculous peritonitis of the wet ascitic type: ... Causes such as bacterial and fungal infections are associated with diffuse abdominal pain, intraperitoneal fluid accumulation, ... intraperitoneal fluid, and regional lymphadenopathy assists in proper diagnosis so clinicians, surgeons, and oncologists can ...
... as well as in fluids and secretions (bile, ascitic fluid). Fischer, Jnos; Ganellin, C. Robin (2006). Analogue-based Drug ...
Conversely, in individuals with very tense ascites, it has been hypothesized that removal of ascitic fluid may improve kidney ... or the removal of large volumes of ascitic fluid by paracentesis from the abdominal cavity without compensating for fluid ... Also, large volume paracentesis-which is the removal of ascites fluid from the abdomen using a needle or catheter in order to ... Spontaneous bacterial peritonitis, which is the infection of ascites fluid, is the most common precipitant of HRS in cirrhotic ...
... that the Combur test UX urine screening test is a very sensitive and specific method for diagnosing SBP in the ascitic fluid of ... The LE test is also used to screen for gonorrhea and for amniotic fluid infections. The combination of the LE test with the ...
... marker protein CA-125 in her blood as well as a significant decrease in the number of cancerous cells in her ascitic fluid. The ...
... infiltration in one or more areas of the gastrointestinal tract or presence of high eosinophil count in ascitic fluid (latter ...
Infected fish show haemorrhagic ulcers on the skin (particularly around the operculum), abundant ascitic fluid in the abdominal ...
... ascitic fluid, or whole blood in order to grow. Numerous published reports also state that the organism is inhibited by the ... Optimizing the best chances for organism recovery should include, the aseptic collection of blood or sterile body fluid(s) into ... HKU33T was found in pus isolated from the abscess of a 38-year-old patient with quinsy and HKU34 from the elbow joint fluid of ...
... which allows ascitic fluid to pass directly into the systemic circulation. Possible complications include: Infection Superior ... A peritoneovenous shunt (also called LeVeen Shunt) is a shunt which drains peritoneal fluid from the peritoneum into veins, ...
... ascitic fluid MeSH A12.207.152 - blood MeSH A12.207.152.200 - fetal blood MeSH A12.207.152.693 - plasma MeSH A12.207.152.846 - ... synovial fluid MeSH A12.207.515 - intracellular fluid MeSH A12.207.739 - nasal lavage fluid MeSH A12.207.927 - urine MeSH ... dentinal fluid MeSH A12.383.500 - gingival crevicular fluid MeSH A12.459.529 - meconium MeSH A12.459.764 - melena The list ... body fluid compartments MeSH A12.207.200 - body water MeSH A12.207.234 - bronchoalveolar lavage fluid MeSH A12.207.270 - ...
... confirmed with the fluid wave/thrill test Ascitic fluid can accumulate as a transudate or an exudate. Amounts of up to 35 ... "fluid thrill" or "fluid wave" (tapping or pushing on one side will generate a wave-like effect through the fluid that can be ... Additionally, the sonographer can make an estimation of the amount of ascitic fluid, and difficult-to-drain ascites may be ... Ascites is the abnormal build-up of fluid in the abdomen. Technically, it is more than 25 ml of fluid in the peritoneal cavity ...
... which allows ascitic fluid to pass directly into the systemic circulation. Possible compilations include bleeding from varices ... In medicine, a shunt is a hole or a small passage that moves, or allows movement of, fluid from one part of the body to another ... A vesico-amniotic shunt is a tube that it is inserted into the unborn baby's bladder to drain the excess fluid into the ... This can result in a reduction in the volume of amniotic fluid, and problems with the development of the baby's lungs and ...
... by excessive production of fluid within the sac, e.g. secondary hydrocele through defective absorption of fluid[citation needed ... Ascites or even ascitic tuberculous peritonitis should be considered if the swellings are bilateral.[citation needed] There is ... The presence of fluid is demonstrated by transillumination. These hydroceles can reach a huge size, containing large amount of ... A hydrocele is an accumulation of serous fluid in a body cavity. A hydrocele testis, the most common form of hydrocele, is the ...
... may also refer to an assessment of an ascitic abdomen. Identifying an organ or a mass in an ascitic abdomen: "Try ... Ballottement is a medical sign which indicates increased fluid in the suprapatellar pouch over the patella at the knee joint. ... This quick movement often displaces the fluid so that your fingertips can briefly touch the surface of the structure through ...
For pleural fluid, this is done by thoracentesis or tube thoracostomy (chest tube); for ascites, with paracentesis or ascitic ... If a large amount of fluid is present, abnormal cells may be detected by cytopathology if this fluid is aspirated with a ... The fluid is perfused for 60 to 120 minutes and then drained. High concentrations of selected drugs are then administered into ... The diagnosis may be suspected based on chest X-ray and CT scan findings, and is confirmed by either examining fluid produced ...
Gastrointestinal Stromal Tumor in Ascitic Fluid: A Case Report Subject Area: Pathology and Cell Biology ... Cytological Findings of Ascitic Fluid with a Malignant Ovarian Steroid Cell Tumor: A Case Report and Literature Review Acta ... Newton A.C.S. Wong, Mark R. Broadbent, Simon Paterson-Brown, Awatif Al-Nafussi; Gastrointestinal Stromal Tumor in Ascitic Fluid ... Diagnosis of Metastatic Carcinoma Using Body Cavity Fluid Specimens: A Comparison of Diagnostic Panels ...
Get all details on Albumin - Ascitic fluid test such as : procedure, preparation, benefits, price, TAT and more. ... Ascitic fluid test online on Bloodoxy at best price. ... ascitic fluid. peritoneal fluid. Glucose - Body Fluid. Glucose ... Details of Albumin - Ascitic fluid What are Albumin - Ascitic fluid?. Ascites are the accumulation of ascitic fluid in the ... Details of Albumin - Ascitic fluid What are Albumin - Ascitic fluid?. Ascites are the accumulation of ascitic fluid in the ...
Find Ascitic Fluid -Culture & Sensitivity Test Information ...
Titre of ascitic fluid/titre of antigen Degree of specific fluorescence with 1:8 antiserum; -- = negative. ... Complement fixation titres for BEF and CSIRO 368 viruses with their respective ascitic fluids. Cross-Immunofluorescence of BEF ...
Ascitic fluid. 79/F. SAMN15325568. N18-0719. 2018 May 2. Human. Blood. 59/F. SAMN15325569. ...
Image: Toxoplasma gondii in mouse ascitic fluid. Smear. (Credit: DPDx). About Toxoplasmosis ...
Ascitic Fluid Bilirubin Concentration as a Key to Choleperitoneum. Runyon, Bruce A. ...
Ascitic Fluid. 2 g. 64 mcg/mL. Cerebrospinal Fluid (in patients with inflamed meninges). 50 mg/kg. 1.8 mcg/mL to 8.0 mcg/mL. ... CEFOBID achieves therapeutic concentrations in the following body tissues and fluids:. Table 2. Tissue or Fluid. Dose. ... Appropriate fluid and electrolyte management, protein supplementation, antibacterial treatment of C. difficile, and surgical ...
Aspiration of the ascitic fluid demonstrated mesothelioma. View Media Gallery Computed tomography (CT) scan in a male Veterans ... There is also free fluid below the diaphragm (yellow arrow). View Media Gallery ...
Ascitic Fluid / metabolism* * Basigin * Biomarkers, Tumor / genetics* * Biomarkers, Tumor / metabolism * Cystadenocarcinoma, ...
Ascites is the build-up of fluid in the space between the lining of the abdomen and abdominal organs. ... Spontaneous bacterial peritonitis (a life-threatening infection of the ascitic fluid). *Hepatorenal syndrome (kidney failure) ... Large amounts of fluid can cause shortness of breath. This happens because the fluid pushes up on the diaphragm, which in turn ... Your doctor may also use a thin needle to withdraw ascites fluid from your belly. The fluid is tested to look for the cause of ...
MeSH Terms: Animals; Ascitic Fluid; Calcium Channel Blockers/toxicity; Calcium/physiology*; Cell Degranulation/drug effects*; ...
Ascitic Fluid Analysis. Ascitic fluid analysis is the gold standard and is required for the confirmation of the diagnosis of ... Ascitic fluid Culture. *Performed before initiating antibiotic therapy by bedside inoculation of ascitIc fluid ≥ 10 mL into ... Perform ascitic fluid cell count and differential ❑ Perform ascitic fluid culture (Inoculated at bedside). ... and as well as in the setting of intestinal perforation into ascitic fluid, the ascitic fluid concentration drops to 0 mg/dL ...
Distributed into bone, bile, pleural fluid, ascitic fluid, and synovial fluid. Only minimal concentrations attained in CSF. ...
There was no ascitic fluid seen. The serum level of CA125, the marker for ovarian cancer, was 27.56 U/mL, beta-human chorionic ... The cut surface of the specimen appears grey in colour, wet and soft and the oedema fluid oozes out with a bulge after cutting ... When the capsule of the left ovary was incised, copious amounts of clear fluid emerged and bulging was observed at the cut ... The ovarian stromal cells are widely separated by copious oedema fluid and atretic follicles may at times be recognized. The ...
... control plates were coated with normal mouse ascitic fluid produced from the parent myeloma cell line. Serum was then added to ... To confirm the potential human risk of Ebola virus-infected dogs, the mechanisms of viral excretion (i.e. body fluids and virus ...
Ascitic fluid cholesterol in malignant and tubercular ascites.. Sood A; Garg R; Kumar R; Chhina RS; Arora S; Gupta R; Bhatia KL ... Usefulness of ascitic fluid cholesterol as a marker for malignant ascites.. Rana SV; Babu SG; Kocchar R. Med Sci Monit; 2005 ... Utility of ascitic fluid analysis in patients with malignancy-related ascites.. Salerno F; Restelli B; Incerti P; Annoni G; ... 9. Ascites in the state of Qatar: aetiology and diagnostic value of ascitic fluid analysis.. Khan FY. Singapore Med J; 2007 May ...
Secondly, people with cirrhosis are at increased risk of ascitic fluid infection. The concern is that LTADs might further ... Faulting, fluids and geohazards within subduction zone forearcs * Faulting, magmatism and fluid flow during volcanic rifting in ... Nurses/carers then drain smaller amounts of fluid (1-2 litres) up to three times a week in the community. LTADs avoid frequent ... putting a thin tube into the abdomen for a few hours and draining 5-15 litres of fluid. This reduces the pain from ascites. ...
The serous fluid of ASCITES, the accumulation of fluids in the PERITONEAL CAVITY.. Terms. Ascitic Fluid Preferred Term Term UI ... use ASCITIC FLUID to search PERITONEAL FLUID 1968-89. History Note. 68; was in Cat A 1968-83; PERITONEAL EFFUSION was ... Peritoneal Fluid Related Concept UI. M000753239. Terms. Peritoneal Fluid Preferred Term Term UI T003583. Date03/31/1989. ... The serous fluid of ASCITES, the accumulation of fluids in the PERITONEAL CAVITY.. Entry Term(s). Peritoneal Effusion ...
Do not remove ascitic, pleural, and pericardial fluid, unless there is the necessity to relieve symptoms such as pulmonary ... As third space fluid returns to the intravascular compartment, a fall in hematocrit and increasing urinary output are observed ... Peripheral and/or pulmonary edema may result if the kidneys are unable to excrete third space fluid as rapidly as it is ... OHSS is characterized by a dramatic increase in vascular permeability, which can result in a rapid accumulation of fluid in the ...
In a previous study we investigated the contribution of ascitic fluid levels of cholesterol and CEA to cytology.16 In the ... Value of carcinoembryonic antigen (CEA) and cholesterol assays of ascitic fluid in cases of inconclusive cytology. J Clin ... Pleural fluid tumour markers in malignant pleural effusion with inconclusive cytologic results. Curr Oncol 2015;22:336-41.doi: ... Diagnostic value of CA 549 in pleural fluid. Comparison with CEA, CA 15.3 and CA 72.4. Lung Cancer 2003;40:289-94.doi:10.1016/ ...
HG-SOC primary cells were obtained from freshly-isolated ascitic fluid of HG-SOC patients undergoing surgery for ovarian tumor ...
Peritoneal cytology of ascitic fluid is highly specific but has relatively low sensitivity, particularly in case of ... Objective: The objectives of this study were to examine the validity of ascitic fluid cytology in the detection of pathological ... to examine the percentage of false positive and false negative results in the cytology of ascitic fluid and to determine the ...
Under a magnification of 1125X, this photomicrograph of a murine ascitic fluid sample revealed the presence of parasitic ...
  • Ascitic fluid analysis is the gold standard for the confirmation of the diagnosis of spontaneous bacterial peritonitis . (wikidoc.org)
  • Ascitic fluid culture is negative in up to 60% of patients with clinical manifestations of spontaneous bacterial peritonitis (SBP). (wikidoc.org)
  • Ascites is the build-up of fluid in the space between the lining of the abdomen and abdominal organs. (medlineplus.gov)
  • Your doctor may also use a thin needle to withdraw ascites fluid from your belly. (medlineplus.gov)
  • The fluid is tested to look for the cause of ascites and to check if the fluid is infected. (medlineplus.gov)
  • Current standard palliative care for untreatable ascites involves coming into hospital for 1-2 days, putting a thin tube into the abdomen for a few hours and draining 5-15 litres of fluid. (southampton.ac.uk)
  • 8. [Concentration of lipids in ascitic fluid and the concentration gradient of albumin in blood and ascites: diagnostic significance]. (nih.gov)
  • 9. Ascites in the state of Qatar: aetiology and diagnostic value of ascitic fluid analysis. (nih.gov)
  • 10. Value of ascitic fibronectin and cholesterol concentration in the differentiation between malignancy-related and non-malignant ascites. (nih.gov)
  • 12. Utility of ascitic fluid analysis in patients with malignancy-related ascites. (nih.gov)
  • 16. (1)H NMR spectroscopy of ascitic fluid: discrimination between malignant and benign ascites and comparison of the results with conventional methods. (nih.gov)
  • 17. [Value of ascitic lipids and sero-ascitic gradient of albumin in the differential diagnosis of ascites]. (nih.gov)
  • 18. Ascitic fluid cholesterol in malignant and tubercular ascites. (nih.gov)
  • 19. Usefulness of ascitic fluid cholesterol as a marker for malignant ascites. (nih.gov)
  • The serous fluid of ASCITES , the accumulation of fluids in the PERITONEAL CAVITY . (nih.gov)
  • Careful attention should be devoted to abdominal palpation to detect abnormalities such as dilated (gas, fluid, or ingesta) loops of bowel or extremely thickened bowel wall, abdominal masses, intraluminal foreign bodies, or ascites. (dvm360.com)
  • There was statistically non-significant difference in ascitic fluid PMN, serum and ascitic fluid hs-CRP before treatment while the difference is significant between both groups regarding them five days after treatment. (who.int)
  • Percent change in serum hs-CRP was equal to that of ascitic fluid PMN. (who.int)
  • Serum and ascitic fluid hs-CRP level can be considered as alternative prognostic markers in cirrhotic patients with SBP. (who.int)
  • VEGF concentrations were measured using an ELISA assay, detecting VEGF 165 isoform, in tumor cyst and/or ascitic fluids and in sera of 86 patients with malignant neoplasms and in 53 patients with benign ovarian neoplasms. (iiarjournals.org)
  • All patients were monitored clinically each month and with ascitic fluid examination at the end of 2 and 6 months if not clinically suspected of recurrence earlier. (nih.gov)
  • Nurses/carers then drain smaller amounts of fluid (1-2 litres) up to three times a week in the community. (southampton.ac.uk)
  • Paracentesis reveals an ascitic fluid with a total white cell count of up to 500 cells/mcL, a high polymorphonuclear ( PMN ) cell count (250/mm 3 more). (wikidoc.org)
  • Peritoneal cytology of ascitic fluid is highly specific but has relatively low sensitivity, particularly in case of endometrioid ovarian carcinoma. (uwi.edu)
  • Tumour diagnosis in pleural fluid is based primarily on effusion cytology. (bmj.com)
  • Conclusions Simultaneous measurement of CEA and cholesterol concentrations in effusion, or at least cholesterol alone, in combination with non-inflammatory fluid cytology, provides additional specific information about neoplastic pleural involvement, and can therefore be used as an adjunct to cytology, above all, in inconclusive cases. (bmj.com)
  • Toxoplasma gondii in mouse ascitic fluid. (cdc.gov)
  • Under a magnification of 1125X, this photomicrograph of a murine ascitic fluid sample revealed the presence of parasitic protozoan, Toxoplasma gondii tachyzoites. (cdc.gov)
  • Secondly, people with cirrhosis are at increased risk of ascitic fluid infection. (southampton.ac.uk)
  • S development of a bacterial infection in the isolated species ascitic fluids are E.coli and peritoneum leading to peritonitis, in spite of the klebsiella. (who.int)
  • A cytology exam of pleural fluid is a laboratory test to detect cancer cells and certain other cells in the area that surrounds the lungs. (blallab.com)
  • 11. Fibronectin, cholesterol and triglycerides ascitic fluid concentration in the prediction of malignancy. (nih.gov)
  • The objectives of this study were to examine the validity of ascitic fluid cytology in the detection of pathological findings, to examine the percentage of false positive and false negative results in the cytology of ascitic fluid and to determine the validity of peritoneal cytology in relation to the histopathological type of the ovarian tumour. (uwi.edu)
  • All patients were subjected to full history taking, Accept Date 2019-02-21 thorough clinical examination, routine laboratory investigation, ultrasonography and ascitic fluid sampling. (who.int)
  • Prophylaxis and antibiotic treatment of SBP requires clinical knowledge for the treatment decisions based mainly on the presence of ascitic fluid, accompanied of risk factors, laboratory indexes such as PMN count and culture results, in order to determine the kind of molecule that will help to the SBP recovery or to amelioration symptoms, always taking care of not exceed the antibiotic consumption and restoring the microbiome imbalance. (intechopen.com)
  • 3. [Fibronectin in the ascitic fluid: its diagnostic significance]. (nih.gov)
  • Ascitic fluid analysis and culture must be performed before initiating antibiotic therapy by bedside inoculation of ascitIc fluid ≥ 10 mL into blood culture bottles. (wikidoc.org)
  • VEGF levels were significantly elevated in the sera and cyst fluids of carcinoma patients compared with patients who had benign neoplasms. (iiarjournals.org)
  • Your doctor will do a physical exam to determine if the swelling is likely due to fluid buildup in your belly. (medlineplus.gov)
  • if you have signs of fluid buildup in the pleural space. (blallab.com)
  • When the capsule of the left ovary was incised, copious amounts of clear fluid emerged and bulging was observed at the cut surface. (who.int)
  • cyst-like accumulations of ascitic fluid upon the surface of the liver. (nih.gov)
  • As more fluid collects, you may have abdominal pain and bloating. (medlineplus.gov)
  • High VEGF levels in ascitic fluids appeared to be significantly associated with shorter disease-free survival and overall survival. (iiarjournals.org)
  • Anaerobic and Patient with Collagen vascular disorders fungal infections of the ascitic fluid are or any form of acute arthritis. (who.int)
  • Hs-CRP is the same gracefully sensitive and 250 in ascitic fluid sample. (who.int)