Ascaris suum: A species of parasitic nematode usually found in domestic pigs and a few other animals. Human infection can also occur, presumably as result of handling pig manure, and can lead to intestinal obstruction.Ascaris: A genus of nematodes of the superfamily ASCARIDOIDEA whose species usually inhabit the intestine.Ascariasis: Infection by nematodes of the genus ASCARIS. Ingestion of infective eggs causes diarrhea and pneumonitis. Its distribution is more prevalent in areas of poor sanitation and where human feces are used for fertilizer.Ascaris lumbricoides: A species of parasitic nematode that is the largest found in the human intestine. Its distribution is worldwide, but it is more prevalent in areas of poor sanitation. Human infection with A. lumbricoides is acquired by swallowing fully embryonated eggs from contaminated soil.Helminth Proteins: Proteins found in any species of helminth.Bephenium Compounds: Analogs or derivatives of bephenium (N,N-dimethyl-N-(2-phenoxyethyl)benzenemethanaminium).Antigens, Helminth: Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.Furylfuramide: Used formerly as antimicrobial food additive. It causes mutations in many cell cultures and may be carcinogenic.Anthelmintics: Agents destructive to parasitic worms. They are used therapeutically in the treatment of HELMINTHIASIS in man and animal.DNA, Helminth: Deoxyribonucleic acid that makes up the genetic material of helminths.Antinematodal Agents: Substances used in the treatment or control of nematode infestations. They are used also in veterinary practice.Larva Migrans, Visceral: A condition produced in man by the prolonged migration of animal nematode larvae in extraintestinal tissues other than skin; characterized by persistent hypereosinophilia, hepatomegaly, and frequently pneumonitis, commonly caused by Toxocara canis and Toxocara cati.Levamisole: An antihelminthic drug that has been tried experimentally in rheumatic disorders where it apparently restores the immune response by increasing macrophage chemotaxis and T-lymphocyte function. Paradoxically, this immune enhancement appears to be beneficial in rheumatoid arthritis where dermatitis, leukopenia, and thrombocytopenia, and nausea and vomiting have been reported as side effects. (From Smith and Reynard, Textbook of Pharmacology, 1991, p435-6)Antibodies, Helminth: Immunoglobulins produced in a response to HELMINTH ANTIGENS.Toxocara canis: A species of parasitic nematode found in the intestine of dogs. Lesions in the brain, liver, eye, kidney, and lung are caused by migrating larvae. In humans, these larvae do not follow normal patterns and may produce visceral larva migrans (LARVA MIGRANS, VISCERAL).RNA, Helminth: Ribonucleic acid in helminths having regulatory and catalytic roles as well as involvement in protein synthesis.Fraxinus: A plant genus of the family OLEACEAE. Members contain secoiridoid glucosides.Tissue Survival: The span of viability of a tissue or an organ.Sentinel Surveillance: Monitoring of rate of occurrence of specific conditions to assess the stability or change in health levels of a population. It is also the study of disease rates in a specific cohort such as in a geographic area or population subgroup to estimate trends in a larger population. (From Last, Dictionary of Epidemiology, 2d ed)Authorship: The profession of writing. Also the identity of the writer as the creator of a literary production.Universities: Educational institutions providing facilities for teaching and research and authorized to grant academic degrees.Information Storage and Retrieval: Organized activities related to the storage, location, search, and retrieval of information.Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Membrane Transport Proteins: Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.Drug Resistance, Multiple, Fungal: The ability of fungi to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance phenotype may be attributed to multiple gene mutations.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Libraries, Digital: Libraries in which a major proportion of the resources are available in machine-readable format, rather than on paper or MICROFORM.Kinetics: The rate dynamics in chemical or physical systems.Malate Dehydrogenase: An enzyme that catalyzes the conversion of (S)-malate and NAD+ to oxaloacetate and NADH. EC 1.1.1.37.Electrophoresis, Capillary: A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)Succinate Dehydrogenase: A flavoprotein containing oxidoreductase that catalyzes the dehydrogenation of SUCCINATE to fumarate. In most eukaryotic organisms this enzyme is a component of mitochondrial electron transport complex II.Iron-Sulfur Proteins: A group of proteins possessing only the iron-sulfur complex as the prosthetic group. These proteins participate in all major pathways of electron transport: photosynthesis, respiration, hydroxylation and bacterial hydrogen and nitrogen fixation.Ubiquinone: A lipid-soluble benzoquinone which is involved in ELECTRON TRANSPORT in mitochondrial preparations. The compound occurs in the majority of aerobic organisms, from bacteria to higher plants and animals.Electron Transport Complex III: A multisubunit enzyme complex that contains CYTOCHROME B GROUP; CYTOCHROME C1; and iron-sulfur centers. It catalyzes the oxidation of ubiquinol to UBIQUINONE, and transfers the electrons to CYTOCHROME C. In MITOCHONDRIA the redox reaction is coupled to the transport of PROTONS across the inner mitochondrial membrane.Succinates: Derivatives of SUCCINIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,4-carboxy terminated aliphatic structure.Trehalase: An enzyme that catalyzes the conversion of alpha,alpha-trehalose and water to D-glucose. EC 3.2.1.28.TrehaloseZygote: The fertilized OVUM resulting from the fusion of a male and a female gamete.Succinic Acid: A water-soluble, colorless crystal with an acid taste that is used as a chemical intermediate, in medicine, the manufacture of lacquers, and to make perfume esters. It is also used in foods as a sequestrant, buffer, and a neutralizing agent. (Hawley's Condensed Chemical Dictionary, 12th ed, p1099; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1851)Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Toxocara: A genus of ascarid nematodes commonly parasitic in the intestines of cats and dogs.Toxocariasis: Infection by round worms of the genus TOXOCARA, usually found in wild and domesticated cats and dogs and foxes, except for the larvae, which may produce visceral and ocular larva migrans in man.Intradermal Tests: Skin tests in which the sensitizer is injected.Skin Tests: Epicutaneous or intradermal application of a sensitizer for demonstration of either delayed or immediate hypersensitivity. Used in diagnosis of hypersensitivity or as a test for cellular immunity.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Mucuna: A plant genus of the family FABACEAE that is the source of mucuna gum.Burseraceae: A plant family of the order Sapindales, subclass Rosidae, class Magnoliopsida. They are resinous trees and shrubs with alternate leaves composed of many leaflets.Haemonchus: A genus of parasitic nematode worms which infest the duodenum and stomach of domestic and wild herbivores, which ingest it with the grasses (POACEAE) they eat. Infestation of man is accidental.Pan troglodytes: The common chimpanzee, a species of the genus Pan, family HOMINIDAE. It lives in Africa, primarily in the tropical rainforests. There are a number of recognized subspecies.Amoebozoa: A supergroup (some say phylum) of ameboid EUKARYOTES, comprising ARCHAMOEBAE; LOBOSEA; and MYCETOZOA.

Nematode pyruvate dehydrogenase kinases: role of the C-terminus in binding to the dihydrolipoyl transacetylase core of the pyruvate dehydrogenase complex. (1/169)

Pyruvate dehydrogenase kinases (PDKs) from the anaerobic parasitic nematode Ascaris suum and the free-living nematode Caenorhabditis elegans were functionally expressed with hexahistidine tags at their N-termini and purified to apparent homogeneity. Both recombinant PDKs (rPDKs) were dimers, were not autophosphorylated and exhibited similar specific activities with the A. suum pyruvate dehydrogenase (E1) as substrate. In addition, the activities of both PDKs were activated by incubation with PDK-depleted A. suum muscle pyruvate dehydrogenase complex (PDC) and were stimulated by NADH and acetyl-CoA. However, the recombinant A. suum PDK (rAPDK) required higher NADH/NAD+ ratios for half-maximal stimulation than the recombinant C. elegans PDK (rCPDK) or values reported for mammalian PDKs, as might be predicted by the more reduced microaerobic mitochondrial environment of the APDK. Limited tryptic digestion of both rPDKs yielded stable fragments truncated at the C-termini (trPDKs). The trPDKs retained their dimeric structure and exhibited substantial PDK activity with the A. suum E1 as substrate, but PDK activity was not activated by incubation with PDK-depleted A. suum PDC or stimulated by elevated NADH/NAD+ or acetyl-CoA/CoA ratios. Direct-binding assays demonstrated that increasing amounts of rCPDK bound to the A. suum PDK-depleted PDC. No additional rCPDK binding was observed at ratios greater than 20 mol of rCPDK/mol of PDC. In contrast, the truncated rCPDK (trCPDK) did not exhibit significant binding to the PDC. Similarly, a truncated form of rCPDK, rCPDK1-334, generated by mutagenesis, exhibited properties similar to those observed for trCPDK. These results suggest that the C-terminus of the PDK is not required for subunit association of the homodimer or catalysis, but instead seems to be involved in the binding of the PDKs to the dihydrolipoyl transacetylase core of the complex.  (+info)

Developmentally regulated telomerase activity is correlated with chromosomal healing during chromatin diminution in Ascaris suum. (2/169)

Telomerase is the ribonucleoprotein complex responsible for the maintenance of the physical ends, or telomeres, of most eukaryotic chromosomes. In this study, telomerase activity has been identified in cell extracts from the nematode Ascaris suum. This parasitic nematode is particularly suited as a model system for the study of telomerase, because it shows the phenomenon of chromatin diminution, consisting of developmentally programmed chromosomal breakage, DNA elimination, and new telomere formation. In vitro, the A. suum telomerase is capable of efficiently recognizing and elongating nontelomeric primers with nematode-specific telomere repeats by using limited homology at the 3' end of the DNA to anneal with the putative telomerase RNA template. The activity of this enzyme is developmentally regulated, and it correlates temporally with the phenomenon of chromatin diminution. It is up-regulated during the first two rounds of embryonic cell divisions, to reach a peak in 4-cell-stage embryos, when three presomatic blastomeres prepare for chromatin diminution. The activity remains high until the beginning of gastrulation, when the last of the presomatic cells undergoes chromatin diminution, and then constantly decreases during further development. In summary, our data strongly argue for a role of this enzyme in chromosome healing during the process of chromatin diminution.  (+info)

Sequence-divergent units of the ABA-1 polyprotein array of the nematode Ascaris suum have similar fatty-acid- and retinol-binding properties but different binding-site environments. (3/169)

Polyproteins comprise long polypeptides that are post-translationally cleaved into proteins of different function, or tandemly repetitive polypeptides which are processed into multiple versions of proteins which are presumed to have the same function. In the latter case the individual units of the polyprotein can differ substantially in sequence. Identity of function between the different units therefore cannot be assumed. Here we have examined the ABA-1 polyprotein allergen of the parasitic nematode Ascaris suum and found it to contain units which show a 50% difference in amino acid sequence. The parasite therefore produces at least two radically different forms of the allergen encoded within the polyprotein array. In fluorescence-based ligand-binding assays, recombinant polypeptides representing the two forms (designated ABA-1A1 and ABA-1B1) showed similar binding affinities for a range of fluorescent active-site probes [retinol, dansylundecanoic acid, dansyl-DL-alpha-amino-octanoic acid, cis-parinaric acid (cPnA)] and for the non-specific hydrophobic surface probe 8-anilinonaphthalene-1-sulphonic acid. However, the molecular environments in the active sites are markedly different, as indicated by disparate fluorescence emission peaks and intensities of bound probes. CD showed that the proteins have similar secondary structures but differ in susceptibility to chemical denaturation/unfolding by guanidinium chloride. Both retain a single conserved tryptophan residue in a characteristic non-polar environment, as revealed by extreme fluorescence blue shift. Thus the gross differences in sequence of the two proteins are not reflected in their ligand-binding specificities but in their binding-site environments.  (+info)

Effect of an orally active Th1/Th2 balance modulator, M50367, on IgE production, eosinophilia, and airway hyperresponsiveness in mice. (4/169)

We have found a novel anti-allergic agent, M50367, which suppresses IgE biosynthesis and eosinophil accumulation in vivo. In this study, we evaluated the ability of M50367 to modulate Th1/Th2 balance in Th2-background BALB/c mice and to inhibit airway hyperresponsiveness in a murine model of atopic asthma. Oral M50367 at 3-30 mg/kg/day exhibited 51 to 73% reduction of IL-4/IL-5 production and 2- to 5-fold augmentation of IFN-gamma production by Ag-stimulated cultured splenocytes of the mice sensitized with DNP-Ascaris. These alterations in Th1/Th2 cytokine production were accompanied by 55-85% suppression of plasma IgE level. Oral M50367 at a dose of 10 mg/kg/day significantly inhibited Ig-independent peritoneal eosinophilia by 54%, which was induced by repeated i.p. injections of Ascaris suum extract. To develop airway hyperresponsiveness caused by allergic airway inflammation, BALB/c mice were sensitized with i.p. OVA injections, followed three times by OVA inhalation. Oral M50367 significantly inhibited the increase in airway reactivity to acetylcholine, together with the elevation of plasma IgE level and pulmonary eosinophilia, which were observed in vehicle-treated mice 1 day after the last inhalation. Moreover, M50367 treatment reduced IL-4 and IL-5 production and tended to enhance IFN-gamma production, not only by cultured splenocytes, but also in bronchoalveolar lavage fluid. These results suggest that M50367 has a modulating ability of Th1/Th2 balance to down-regulate Th2 response in the circulating system as well as at the sites of inflammation, and may be beneficial for the treatment of allergic disorders such as atopic asthma.  (+info)

Localized depolymerization of the major sperm protein cytoskeleton correlates with the forward movement of the cell body in the amoeboid movement of nematode sperm. (5/169)

The major sperm protein (MSP)-based amoeboid motility of Ascaris suum sperm requires coordinated lamellipodial protrusion and cell body retraction. In these cells, protrusion and retraction are tightly coupled to the assembly and disassembly of the cytoskeleton at opposite ends of the lamellipodium. Although polymerization along the leading edge appears to drive protrusion, the behavior of sperm tethered to the substrate showed that an additional force is required to pull the cell body forward. To examine the mechanism of cell body movement, we used pH to uncouple cytoskeletal polymerization and depolymerization. In sperm treated with pH 6.75 buffer, protrusion of the leading edge slowed dramatically while both cytoskeletal disassembly at the base of the lamellipodium and cell body retraction continued. At pH 6.35, the cytoskeleton pulled away from the leading edge and receded through the lamellipodium as its disassembly at the cell body continued. The cytoskeleton disassembled rapidly and completely in cells treated at pH 5.5, but reformed when the cells were washed with physiological buffer. Cytoskeletal reassembly occurred at the lamellipodial margin and caused membrane protrusion, but the cell body did not move until the cytoskeleton was rebuilt and depolymerization resumed. These results indicate that cell body retraction is mediated by tension in the cytoskeleton, correlated with MSP depolymerization at the base of the lamellipodium.  (+info)

1H NMR investigation of the distal hydrogen bonding network and ligand tilt in the cyanomet complex of oxygen-avid Ascaris suum hemoglobin. (6/169)

The O(2)-avid hemoglobin from the parasitic nematode Ascaris suum exhibits one of the slowest known O(2) off rates. Solution (1)H NMR has been used to investigate the electronic and molecular structural properties of the active site for the cyano-met derivative of the recombinant first domain of this protein. Assignment of the heme, axial His, and majority of the residues in contact with the heme reveals a molecular structure that is the same as reported in the A. suum HbO(2) crystal structure (Yang, J., Kloek, A., Goldberg, D. E., and Mathews, F. S. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 4224-4228) with the exception that the heme in solution is rotated by 180 degrees about the alpha,gamma-meso axis relative to that in the crystal. The observed dipolar shifts, together with the crystal coordinates of HbO(2), provide the orientation of the magnetic axes in the molecular framework. The major magnetic axis, which correlates with the Fe-CN vector, is found oriented approximately 30 degrees away from the heme normal and indicates significant steric tilt because of interaction with Tyr(30)(B10). The three side chain labile protons for the distal residues Tyr(30)(B10) and Gln(64)(E7) were identified, and their relaxation, dipolar shifts, and nuclear Overhauser effects to adjacent residues used to place them in the distal pocket. It is shown that these two distal residues exhibit the same orientations ideal for H bonding to the ligand and to each other, as found in the A. suum HbO(2) crystal. It is concluded that the ligated cyanide participates in the same distal H bonding network as ligated O(2). The combination of the strong steric tilt of the bound cyanide and slow ring reorientation of the Tyr(30)(B10) side chain supports a crowded and constrained distal pocket.  (+info)

Effect of the injection of an extract of Ascaris suum on macrophage activation during the early phase of Mycobacterium bovis BCG infection in C57Bl/6 mice. (7/169)

Injection of an Ascaris suum extract (Asc) affects both the humoral and cellular immune responses to unrelated antigens when it is co-administered with these antigens. In the present study we evaluated the effect of Asc on macrophage activation in the early phase of Mycobacterium bovis BCG (Pasteur strain TMCC 1173) infection in C57Bl/6 mice. C57Bl/6 mice were injected intraperitoneally (ip) with 0.1 mg BCG (BCG group) or BCG plus 1 mg Asc (BCG + Asc group). The peritoneal exudates were obtained at 2, 7 and 14 days after infection. The numbers of IFN-gamma-secreting cells were assessed by the ELISPOT assay. Nitric oxide (NO) production was measured by the Griess method and by the evaluation of NADPH diaphorase activity in the peritoneal exudates. The administration of Asc extract increased NADPH diaphorase activity (2 days: control = 0, BCG = 7%, BCG + Asc = 13%, and Asc = 4%; 7 days: control = 4, BCG = 13%, BCG + Asc = 21%, and Asc = 4.5%) and TNF-alpha levels (mean +/- SD; 2 days: control = 0, BCG = 169 +/- 13, BCG + Asc = 202 +/- 37, and Asc = 0; 7 days: control = 0, BCG = 545 +/- 15.5, BCG + Asc = 2206 +/- 160.6, and Asc = 126 +/- 26; 14 days: control = 10 +/- 1.45, BCG = 9 +/- 1.15, BCG + Asc = 126 +/- 18, and Asc = 880 +/- 47.67 pg/ml) in the early phase of BCG infection. Low levels of NO production were detected at 2 and 7 days after BCG infection, increasing at 14 days (mean +/- SD; 2 days: control = 0, BCG = 3.7 +/- 1.59, BCG + Asc = 0.82 +/- 0.005, Asc = 0.48 +/- 0.33; 7 days: control = 0, BCG = 2.78 +/- 1.54, BCG + Asc = 3.07 +/- 1.05, Asc = 0; 14 days: control = 0, BCG = 9.05 +/- 0.53, BCG + Asc = 9.61 +/- 0.81, Asc = 10.5 +/- 0.2 (2 x 10(6)) cells/ml). Furthermore, we also observed that Asc co-injection induced a decrease of BCG-colony-forming units (CFU) in the spleens of BCG-infected mice during the first week of infection (mean +/- SD; 2 days: BCG = 1.13 +/- 0.07 and BCG + Asc = 0.798 +/- 0.305; 7 days: BCG = 1.375 +/- 0. 194 and BCG + Asc = 0.548 +/- 0.0226; 14 days: BCG = 0.473 +/- 0.184 and BCG + Asc = 0.675 +/- 0.065 (x 10(2)) CFU). The present data suggest that Asc induces the enhancement of the immune response in the early phase of BCG infection.  (+info)

Phosphocholine-containing, zwitterionic glycosphingolipids of adult Onchocerca volvulus as highly conserved antigenic structures of parasitic nematodes. (8/169)

Human Onchocerca volvulus infection sera were found to recognize zwitterionic glycolipids of O. volvulus and to cross-react with those of other parasitic nematodes (Ascaris suum, Setaria digitata and Litomosoides sigmodontis). By the use of an epitope-specific monoclonal antibody, zwitterionic glycolipids of all these nematode species were observed to contain the antigenic determinant phosphocholine. A hyperimmune serum specific for arthro-series glycolipid structures reacted with the various neutral glycolipids of all these nematodes, which demonstrated that their oligosaccharide moieties belonged to the arthro-series of protostomial glycolipids. These results indicated that arthro-series glycosphingolipids carrying, in part, phosphocholine substituents, represent highly conserved, antigenic glycolipid markers of parasitic nematodes. Three glycolipid components of the O. volvulus zwitterionic fraction were structurally characterized by matrix-assisted laser-desorption/ionization time-of-flight MS, methylation analysis and exoglycosidase treatment. Their chemical structures were elucidated to be phosphocholine-6GlcNAc(beta1-3)Man(beta1-4)Glc(1-1)ceramide, GalNAc(beta1-4)[phosphocholine-6]GlcNAc(beta1-3)Man(beta1-4)Glc(1-1) ceramide and Gal(alpha1-3)GalNAc(beta1-4)[phosphocholine-6]GlcNAc(beta1-3)Man(beta 1-4)Glc(1-1)ceramide for the zwitterionic ceramide tri-, tetra- and penta-hexosides respectively. The ceramide composition was found to be dominated by 2-hydroxylated docosanoic (C(22h:0)), tricosanoic (C(23h:0)) and tetracosanoic (C(24h:0)) acids, and C(17) sphingosine (C(d17:1)) (where (h) is hydroxylated and (d) is dihydroxylated).  (+info)

BACKGROUND: Caenorhabditis elegans has traditionally been used as a model for studying nematode biology, but its small size limits the ability for researchers to perform some experiments such as high-throughput tissue-specific gene expression studies. However, the dissection of individual tissues is possible in the parasitic nematode Ascaris suum due to its relatively large size. Here, we take advantage of the recent genome sequencing of Ascaris suum and the ability to physically dissect its separate tissues to produce a wide-scale tissue-specific nematode RNA-seq datasets, including data on three non-reproductive tissues (head, pharynx, and intestine) in both male and female worms, as well as four reproductive tissues (testis, seminal vesicle, ovary, and uterus). We obtained fundamental information about the biology of diverse cell types and potential interactions among tissues within this multicellular organism. METHODOLOGY/PRINCIPAL FINDINGS: Overexpression and functional enrichment analyses ...
Williamson, S. M., Robertson, A. P., Brown, L., Williams, T., Woods, D. J., Martin, R. J., Sattelle, D. B. and Wolstenholme, A., 2009. The nicotinic acetylcholine receptors of the parasitic nematode ascaris suum: formation of two distinct drug targets by varying the relative expression levels of two subunits. PLoS Pathogens ...
The levels of trehalose and the activity of trehalase during the development of Ascaris suum eggs were investigated. The level of trehalose in the zygote was high (2.96 ± 0.07 mg/g). During cleavage of eggs, it decreased (0.91 ± 0.35 mg/g). A higher concentration of the sugar was recorded at the blastula and during gastrulation, but it did not reach the uncleaved eggs level. In the early motile larvae, the concentration of trehalose was high (4.58 ± 2.01 mg/g). It decreased with development of L1 larvae (3.10 ± 1.47 mg/g). A rapid increase in trehalose reserves was observed between the L1 and L2 stages. The highest content of trehalose was found in invasive L2 larvae (5.78 ± 1.39 mg/g).. The activity of trehalase at the zygote stage was high (560.22 ± 322.31 U/mg). It decreased at the beginning of cleavage. It was the lowest at the 4-6-cell stage (189.76 ± 114.97 U/mg). An increase in the enzyme activity occurred after reaching the blastula stage (348.44 ± 343.34 U/mg). The highest ...
A procedure for the isolation of malic enzyme from muscle tissue of the roundworm Ascaris suum is described. The fractionation method yields relatively large quantities of the enzyme,with a specific activity of fifteen moles of malate converted to pyruvate and carbon dioxide per min per mg at 25º. Homogeneity was established with analytical ultracentrifugation, zone electrophoresis, isoelectric focusing, and rechromatography. The molecular weight of the enzyme was 250,000, and it is dissociated under several conditions into four identical monomers of 64,000 daltons. The enzyme exists as a single electrophoretic form and prefers manganous and NAD over other cations and NADP. Ammonium sulfate competes with manganous for the active site and titration with DTNB yields eight thiol groups per mole. Titration of the first four thiol groups is accompanied by a complete loss in enzyme activity. Equilibrium dialysis, product inhibition, and initial velocity studies suggest a rapid-equilibrium random sequential
Read "Protective immunity elicited by the nematode-conserved As37 recombinant protein against Ascaris suum infection, PLoS Neglected Tropical Diseases" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
O setor suinícola brasileiro tem enfrentado nos últimos anos, barreiras para exportação da carne, principalmente relacionadas a questões sanitárias e ao bem-estar animal. Em relação às questões sanitárias, grande destaque tem sido dado às parasitoses intestinais causadas por helmintos e às falhas no processo de vermifugação decorrentes da resistência a antiparasitários. Portanto, é crescente a necessidade de se estudar o perfil epidemiológico dos parasitas que acometem os animais e a partir daí avaliar estratégias de controle alternativos e sustentáveis.O objetivo desse trabalho foi avaliar métodos alternativos de controle para Ascaris suum na produção de suínos. O trabalho foi dividido em quatro estudos, realizados em etapas distintas. No primeiro objetivou-se avaliar a epidemiologia do A. suum em rebanhos suinícolas localizado na micro-região de Ponte Nova, na Zona da Mata de Minas Gerais - Brasil. No segundo ensaio, foi avaliada a constituição fitoquímica do ...
Abstract: Ascaris suum extract (Asc) down-regulates the immune response to ovalbumin (OA) as well as preferentially stimulates a Th2 response to its own components, which is responsible for the suppression of the OA-specific Th1 response. IL-4 plus IL-10-specific monoclonal antibody treatment of mice before immunization with OA + Asc abolishes this suppression. The immunosuppressive activity of Asc is due to high MW proteins (PI) that are obtained by gel filtration chromatography. Although PI stimulates IL-4 and IL-10 secretion, IgE and anaphylactic IgG1 antibodies against PI are only obtained in IL-12 or IFN-g-/-deprived mice. In this work, we investigated the role of IL-12 and IFN-g in the suppression exerted by PI on an unrelated immune response. IFN-g- or IL-12-deficient (IL-12-/- / IFN-g-/-) or wild-type mice were immunized with OA or OA + PI in complete Freunds adjuvant and challenged in the hind footpad with aggregated OA after 8 days. Immediate and delayed-type-hypersensitivity (DTH) ...
The malic enzyme gene of Ascaris suum was cloned into the vector pTRC99a in two forms encoding alternative amino-termini. The resulting plasmids, pMEA1 and pMEA2, were introduced into Escherichia coli...
Google Scholar Page. Peer reviewed papers since 2014. "**" Denotes a student that Dr. Karriker mentored, served as major professor, served as graduate committee member, or served as professional (DVM) curriculum advisor during the study.. Pittman J**, Myers G, Stalder K, Karriker L. 2015. Effect of fenbendazole on shedding and embryonation of Ascaris suum eggs from naturally infected sows. Journal of Swine Health and Production. 23(5):252-263.. Day D**, Sparks J**, Karriker L, Stalder K, Wulf L, Zhang J, Kinyon J, Stock M, Gehring R, Wang C, Ellingson J, Coetzee J. 2015. Impact of an experimental PRRSV and Streptococcus suis coinfection on the pharmacokinetics of ceftiofur hydrochloride after intramuscular injection in pigs. Journal of Veterinary Pharmacology and Therapeutics. doi: 10.1111/jvp.12209. [Epub ahead of print].. Thomas P**, Karriker L, Ramirez A, Zhang J, Ellingson J, Crawford K**, Bates J**, Hammen K, Holtkamp D. 2015. Evaluation of time and temperature sufficient to inactivate ...
Staff publications is the digital repository of Wageningen University & Research. Staff publications contains references to publications authored by Wageningen University staff from 1976 onward.. Publications authored by the staff of the Research Institutes are available from 1995 onwards.. Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.. We have a manual that explains all the features ...
Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
WormBase ParaSite is an open access resource providing genome sequences, genome browsers, semi-automatic annotation and comparative genomics analysis for nematode and platyhelminth parasites (helminths).
WormBase ParaSite is an open access resource providing genome sequences, genome browsers, semi-automatic annotation and comparative genomics analysis for nematode and platyhelminth parasites (helminths).
I De Baere, L Liu, L Moens, J Van Beeumen, C Gielens, J Richelle, C Trotman, J Finch, M Gerstein, M Perutz (1992). Proc Natl Acad Sci U S A 89: 4638-42 ...
Versteeg L, Le Guezennec X, Zhan B, et al. Transferring Luminex® cytokine assays to a wall-less plate technology: Validation and comparison study with plasma and cell culture supernatants. Journal of Immunological Methods. 2017;440:74-82.. Le Burel S, Thépenier C, Boutin L, lataillade j-j, Peltzer J. Effect of Mesenchymal Stromal Cells on T cells in septic context: immuno-suppression or immuno-stimulation? Stem Cells and Development. 2017.. Wei J, Versteeg L, Liu Z, et al. Yeast-expressed recombinant As16 protects mice against Ascaris suum infection through induction of a Th2-skewed immune response. PLOS Neglected Tropical Diseases. 2017;11(7):e0005769.. Tan-Garcia A, Wai L-E, Zheng D, et al. Intrahepatic CD206+ macrophages contribute to inflammation in advanced viral-related liver disease. Journal of Hepatology. 2017.. See P, Dutertre C-A, Chen J, et al. Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 2017;356(6342).. McGovern N, Shin A, Low G, et ...
Lewis, R. and Behnke, Jerzy M. and Cassidy, J.P. and Stafford, P. and Murray, N. and Holland, Celia V. (2007) The migration of Ascaris suum larvae, and the associated pulmonary inflammatory response in susceptible C57BL/6j and resistant CBA/Ca mice. Parasitology, 134 (9). pp. 1301-1314. ISSN 0031-1820 ...
en] The objective of this study was to investigate whether high-dose inhaled fluticasone propionate (FP), alone or in combination with salmeterol (SAL), is as effective as oral prednisolone in reducing airway inflammation and obstruction in cats with experimentally-induced acute asthma. Six cats sensitised to Ascaris suum (AS) were enrolled in a prospective controlled therapeutic trial and underwent four aerosol challenges, at 1-month intervals with AS allergen. The allergen - stimulated animals received four consecutive days treatment with either oral prednisolone at 1mg/kg twice daily, 500μg of FP inhaled twice daily, or a combination of FP/SAL at 500μg/50μg inhaled twice daily, respectively, according to a randomised cross-over design. Treatment-related changes in lung function, airway responsiveness (AR) and bronchoalveolar lavage fluid (BALF) cytology were assessed. Barometric whole-body plethysmography (BWBP) was used for the assessment of respiratory variables and AR. No significant ...
Jex, Aaron R., Liu, Shiping, Li, Bo, Young, Neil D., Hall, Ross S., Li, Yingrui, Yang, Lifeng, Zeng, Na, Xu, Xun, Xiong, Zijun, Chen, Fangyuan, Wu, Xuan, Zhang, Guojie, Fang, Xiaodong, Kang, Yi, Anderson, Garry A., Harris, Todd W., Campbell, Bronwyn E., Vlaminck, Johnny, Wang, Tao, Cantacessi, Cinzia, Schwarz, Erich M., Ranganathan, Shoba, Geldhof, Peter, Nejsum, Peter, Sternberg, Paul W., Yang, Huanming, Wang, Jun, Wang, Jian, and Gasser, Robin B. (2011) Ascaris suum draft genome. Nature, ...
Enhanced light micrograph of roundworm (ascaris) cells in the metaphase stage of mitosis. Mitosis is the cell division of a nucleus, typically consisting of four stages: prophase, metaphase, anaphase, and telophase. It usually results in two new nuclei, each of which contains a complete copy of the parental chromosomes. Magnification 160x at 35mm. - Stock Image C028/2378
braziliense and Uncinaria stenocephala infect each cats and puppies. Hookworms tend to be smaller than the enormous roundworms Ascaris lumbricoides and so lead to significantly less tissue harm and obstruction. The most important danger of hookworm an infection is anemia, secondary to lack of iron (and protein) in the intestine. The worms suck blood voraciously and harm the mucosa. Nonetheless, the blood decline within the stools just isnt visibly evident ...
In control arm, the tumor grew the fastest within the first couple weeks. As the tumor enlarged, we noticed a decline in growth rate of these tumors. In the control arm, the tumor growth rate was significantly higher in the first 15 days compared to the second 15 days. The tumor volume doubling time changed from 103 h in the first 15 days to 144 h in the second 15 days (P , 0.01). However, in the 2 mg T and 5 mg T arms, the opposite results were found. The data demonstrated that in 2 mg T and 5 mg T arms, the tumor growth rate was significantly lower in the first 15 days compared to the second 15 days, (184 h vs 133 h in 2 mg T arm, P , 0.05, 206 h vs 140 h in 5 mg T arm, P , 0.01). When comparing the growth rates of the control arm and the 2 mg T and 5 mg T arms in the first 15 days, the growth rates were much higher in the control arm (P , 0.01). However, in the second 15 days there was no significant difference in tumor growth rates in the control arm, 2 mg T arm, and 5 mg T arms (Fig. 4). ...
By design, sperm cells are small, motile, and transcriptionally inactive. Thus a key step in the developmental program of making a sperm involves prepackaging components that need to be synthesized before the turnoff of transcription and stored for later use. Nematode spermatogenesis provides an extreme example of this process; bulk transcription ceases prior to the meiotic divisions and translation ceases immediately after anaphase II. In particular, spermatocytes must synthesize and prepackage a small filament forming protein called the major sperm protein (MSP). Within mature nematode sperm, MSP serves both as the protein that drives sperm motility and as a signaling protein that triggers oocyte maturation and the physical process of ovulation. Our lab has recently identified several factors involved in both the assembly and sequestration of MSP into large, macromolecular structures called fibrous bodies. Furthermore, in a link to our cell division interests, mutants with defects in MSP ...
When I was about 3 to about 5 years old there was a large group of neighborhood kids who played together in the field behind my house. I was one of the kids that was always there, with my two big sisters and my older brother. One of the songs we sang was The Worms Crawl in, The Worms Crawl Out. We would all hold hands to make a long chain. The person at one end would put their free arm stretched out against a tree. The person at the other end of the chain would arc around and go under the "tree" persons arm and the whole chain would follow. We would sing, "The worms crawl in, the worms crawl out" while we were doing this.. Ive been trying to remember the rest of the lyrics we sang, so I could post it as a Halloween song. It seems so fitting! But, try as I might, I couldnt quite get it all.. Finally, I spoke to my "big" sister Dawn. Shes one of the experts I consult about the songs I sang in my childhood. She recalls them better, since I was so young during that time period (shes five years ...
Eicha (Lamentations) 2 - AIN fecit Dominus quae cogitavit conplevit sermonem suum quem praeceperat a diebus antiquis destruxit et non pepercit et laetificavit super te inimicum et exaltavit cornu hostium tuorum
sert s kolera (pneumo-enteritis suum), fert z betegs g, melyet Amerik ban m r 1833 ta ismernek s innen Eur p ba is behurcolt k. El sz r Angli ban (1862) l pett fel, k s bb Hollandi ban, N me
Venting a crawl space with outside air brings summer humidity and cold winter air into your home, which leads to damaging mold and rot underneath your home. The Crawl-O-Sphere™ Crawl Space Fan provides...
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Can you name the four letter words in this themed word ladder? Test your knowledge on this just for fun quiz to see how you do and compare your score to others. Quiz by Crawl
Watching your baby and toddler master development milestones to help take those first steps is a joy. Heres how to help your little one on the way
Abstract The specificity and sensitivity of adult and larval somatic antigens, and perienteric fluid of Toxocara canis and Ascaris suum were investigated by using intradermal skin tests in guinea pigs. These animals were infected with low doses (0.01, 0.1 or 1.0 egg/g) of these helminths. Toxocara larval antigen (TL) induced larger reactions in Toxocara-infected animals than did the other antigens, suggesting a superior sensitivity for this antigen. In addition, Ascaris perienteric fluid (AP) provoked skin responses in these animals of a magnitude similar to those induced by TL. The reciprocal relationship, i.e., comparable reactivity of AP and TL in Ascaris-infected animals, was not apparent. In general, Ascaris larval antigen and AP elicited larger intradermal reactions than other antigens in Ascaris-infected animals. The results of this study indicated no superiority of adult antigens in differentiating Ascaris and Toxocara infections.
Sentinel Chambers are loaded with 100,000 Ascaris eggs and wastewater or biosolids taken directly from the treatment plant to be tested. Fine mesh screens on the ends of the Sentinel Chamber hold the eggs within the chamber and at the same time allow the interior to equilibrate with the surrounding environment. After the Sentinel Chamber is in the test environment for the requested period, the eggs are easily recovered to determine viability following disinfection ...
TY - JOUR. T1 - Dephosphorylation of major sperm protein (MSP) fiber protein 3 by protein phosphatase 2A during cell body retraction in the MSP-based amoeboid motility of Ascaris sperm. AU - Yi, Kexi. AU - Wang, Xu. AU - Emmett, Mark. AU - Marshall, Alan G.. AU - Stewart, Murray. AU - Roberts, Thomas M.. PY - 2009/7/15. Y1 - 2009/7/15. N2 - The crawling movement of nematode sperm requires coordination of leading edge protrusion with cell body retraction, both of which are powered by modulation of a cytoskeleton based on major sperm protein (MSP) filaments. We used a cell-free in vitro motility system in which both protrusion and retraction can be reconstituted, to identify two proteins involved in cell body retraction. Pharmacological and depletion-add back assays showed that retraction was triggered by a putative protein phosphatase 2A (PP2A, a Ser/Thr phosphatase activated by tyrosine dephosphorylation). Immunofluorescence showed that PP2A was present in the cell body and was concentrated at ...
For many people in the west, a nematode infestation is an unpleasant, but rare event. Unfortunately, the same cannot be said for the inhabitants of many less developed countries, where nematodes are rife and cause many crippling diseases. Fortunately, modern medicine is equipped with a battery of drugs to rid patients of unwanted parasites. But the threat remains that the nematodes will eventually outsmart the pharmacologists and become resistant to the three major classes of drug currently in use. Which is why Richard Martin is keen to understand how one of these nematode targeting drugs, levamisole, attacks the nematode, Ascaris suum. He hopes this will help us to design new therapies to keep medicine ahead of the nematode in the race to beat resistance (p. 3979).. Martin explains that many of the drugs, called anthelmic drugs, which are currently so effective in treating roundworm infections, were originally developed for use in western veterinary practice. He explains that many of these ...
Both the Tsimane Amazonian Panel Study[6] and The Tsimane Health and Life History Project have studied the Tsimane since 2002.[7] Among other things, it appears that they do not develop heart disease as they age in the same ways as people in the developed world.[8] [9] The average Tsimané woman has nine children in her lifetime. A study of 983 Tsimané women found that 70% were infected with the parasitic roundworm Ascaris lumbricoides, which is believed to have increased their fertility rate by suppressing their immune system, leading to two additional children over the course of a lifetime.[10] ...
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Tjernlund Products is your source for crawl space ventilation. We carry products ranging from a room to room fan, dryer booster fan, a chimney fan, and more. Don�t worry about damp crawl space moisture again with our dryer boosting fan.
Wet n Wild Crafty Kitten Crawl the Line Kit - A cat is nothing without its spots, with the Wet n Wild Crafty Kitten Crawl the Line Kit you can release your inner feline. This palette features 6 gl
Question - Child born premature not gaining required weight. Unable to sit and crawl. Having solids and sleeping properly . Ask a Doctor about diagnosis, treatment and medication for Weight loss, Ask a Pediatrician
Read the original post The 3 Best Crawl Space Dehumidifiers Reviews (Stop Mold, Mildew, and Bacteria Growth) on BabyDotDot - Baby Guide For Awesome Parents & More. You have taken care of all parts of the house, inside and outside. But there is a space underneath the floors or roof that is not always in…
Archived from groups: rec.games.roguelike.misc (http://www.tomshardware.com/site/forums-usenet-faq.html)HihoFollowing Suggestion for Future Versions of Crawl:One should be able to create something lik...
Front crawl (FC) and back crawl (BC) are similar in terms of having alternating contributions of the arms combined with a six beat kick. However, the reason for the faster swimming times of FC than BC has not yet been ...
Im still alive! - Sorry to have been so absent for so long. Its been a lot this beginning of the year. The good news is everyone I know and love is still alive so far - a p... ...
I woke up yesterday and had a not so nice encounter involving my head being perched on the side of the toilet bowl resulting in me making it in to work to come home again and crawl into bed. The tummy bug gremlins have infiltrated my system. And it seems the only thing I can think…
The in vitro anthelmintic evaluation of the crude extracts and solvent partitions on Pheretima posthuma and Ascarissuum (eggs and L2 stage) respectively showed significant results at the same concentration (10, 20, 40 and 80 mg/ml). This study provides a clear evidence for usage of Mucuna pruriens and Canarium schweinfurthii as an anthelmintic. The most potent ovicidal partition of C. schweinfurthii (Engl) leaves and bark and leaves of M. pruriens (DC) is hexane (100.00 ± 0.33), chloroform (98.20 ± 0.12) and chloroform (98.70 ± 0.88) partitioned crude respectively at 80 mg/ml while the most potent larvicidal partition is hexane (0.06%), chloroform (0.1%) and chloroform (0.2%). The active compounds are predominantly in the non-polar solvent which supports the tegumental diffusing activity of the compounds.
The examination was performed on 81 fecal samples, collected from swine, which came from three farms located in Dolnoslaskie and Wielkopolskie provinces, and on 42 samples from dogs from experimental breeding and dogs belongs to private owners. With both methods, as well F lleborns method as Fecalyzer method, infection in swine of coccidia (Coccidia) and nematods: Oesophagostomum spp. and Ascaris suum was detected. While, in dog feces the eggs of large roundworm Toxascaris leonina and whipworm Trichuris vulpis was detected. Method with Fecalyzer turned out slightly more sensitive than F lleborns method in case of infection of A. suum in swine and T. vulpis in dogs, and differences in prevalence of infection with this parasites amounted suitably 1.2% and 2.4%. Besides, method with Fecalyzer was characterized by smaller labour intensity and was less time-consuming than F lleborns method. Optimal, recommended time after which should be made result reading in this method, should be at least 10 ...
The polyprotein allergens/antigens of nematodes (NPAs) are the only lipid binding proteins known to be produced as polyproteins. Cleavage of the large polyprotein precursors at regularly spaced proteinase cleavage sites produces 10 or 11 individual protein units of similar to 15 kDa. The sequences of these units are highly diverse within and between species, but there are five absolutely or strongly conserved amino acid positions (Trp 15, Gln20, Leu42, Cys64, and Cys120). We have tested the role of these signature amino acids by mutational or chemical alteration of the ABA-1 protein of Ascaris, and examined the resulting modified proteins for perturbations of their lipid binding activities and structural integrity. Substitution of Trp15 and Gln20 both affect the stability of the protein in terms of resistance to thermal or chemical denaturation, but the ligand binding function is unaffected. Mutation of Leu42, however, disrupts both the proteins structural stability and functional integrity, as ...
Roundworms (nematodes) are common in poultry, waterfowl, and wild birds. Species of roundworms that affect poultry include species of large roundworms (Ascaris sp., also known as ascarids), species of small roundworms (Capillaria sp., also known as capillary worms or threadworms), and cecal worms (Heterakis gallinarum). Roundworms can cause significant damage to the organ(s) they infest. Most roundworms affect the digestive tract; others affect the trachea (windpipe) or eyes.. Large roundworms are the most damaging of the worms common to backyard flocks. A severe infestation can cause a reduction in nutrient absorption, intestinal blockage, and death. Easily seen with the naked eye, large roundworms are about the thickness of a pencil lead and grow to 4-1/2 inches long. Occasionally, they migrate up a hens reproductive tract and become included in a developing egg. The life cycle of a roundworm is direct; that is, worm eggs are passed in the droppings of infected birds and then directly to ...
For poultry, pigeons and pigs. For the treatment of Round Worms (Ascaridia Galli) in Poultry (Ascaridia columbae) in Pigeons; Round Worms (Ascaris suum)...
Edouard Joseph Marie Van Beneden (Leuven, 5 March 1846 - Liège, 28 April 1910), cytologist and marine biologist. He was professor of zoology at the University of Liège.[1] He contributed to cytology and genetics by his work on the roundworm Ascaris. In this work he discovered how chromosomes combined during meiosis, during the production of gametes.[2]. Beneden discovered, with Walther Flemming and Eduard Strasburger, the essential facts of mitosis. In contrast to meiosis, the double (diploid) set of chromosomes in daughter cells is identical to those in the parent cells. Excepting rare accidents, there is no change in the genetic apparatus. In meiosis, only one set of chromosomes ends up in each gamete, a condition called haploid. ...
352 THE WILL IN NATURE.. et deinceps corpus movendo foret? At extemplo contra hanc magicam motricem obicies istam esse intra concretum sibi suumque hospitium naturale, idcirco hanc, etsi magicam vocitemus, tantum erit nominis detorsio et abusus, siquidem vera et superstitiosa magica non ex anima basin desumit; cum eadem haec nil quidquam valeat, extra corpus suum movere altera re aut ciere. Respondeo vim et magicam illam naturalem animae, quae extra se agat virtute imaginis Dei latere iam obscuram in homine velut obdormire (post praevaricationem) excitationisque indigam: quae eadem, utut somnolenta, ac velut ebria, alioqui sit in nobis cottidie: sufficit tamen ad obeunda munia in corpore suo: dormit itaque scientia et potestas magica et solo nutu actrix in homine. [The soul, which is absolutely and entirely spirit, would never be able to move or excite the breath of life (which is thus physical and material) and still less flesh and bones, unless a certain power, naturally inherent in it, yet ...
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... 1518518 clouded the Bringer of Life (level 23, 204/218 HPs) Began as a Deep Dwarf Assassin on Sept 29, 2011. Was the Champion of Elyvilon. Escaped with the Orb ... and 3 runes on Sept 30, 2011! The game lasted 06:59:47 (66032 turns). clouded the Bringer of Life (Deep Dwarf Assassin) Turns: 66032, Time: 06:59:47 HP 204/218 AC 36 Str 27 XL: 23 Next: 59% MP 38/42 EV 13 Int 18 God: Elyvilon [******] Gold 5143 SH 43 Dex 19 Spells: 2 memorised, 27 levels left Res.Fire : + + . See Invis. : . a - +8,+9 broad axe (freeze) Res.Cold : x . . Warding : . . R - +8 dragon armour Life Prot.: + . . Conserve : + U - -1 large shield Res.Acid. : . . . Res.Corr. : . S - +2 helmet of Obohoheh {Str+5 Dex+1} Res.Poison: + Clarity : . J - +1 cloak "Strowk" {+Blink} Res.Elec. : + Spirit.Shd : . l - +1 pair of dwarf gloves Sust.Abil.: . . Stasis : . L - +2 pair of boots of the Monkeys Uncle {rE Res.Mut. : . Ctrl.Telep.: x v - amulet of conservation ...
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Parasitic nematodes, also know as roundworms, cause diseases in a variety of organs. Their larva can cause disease by migrating through the body.
I have a one year old house on a crawl space that is lined with plastic. The indoor humidity is 60-70%. Have a central heat and air unit outside, gas heat, electric air, ducted through crawl space. I live in TN where it is humid. A Ultra air 90 whole house dehumidifier has been recommended. My concerns are that this will be placed in the crawl space and I am concerned about mold growth on the coils of the unit. Has this been a problem with anyone else? Also, the diagrams for installation
The WebCrawler worker is started with one input bulk that contains records with URLS to crawl. (The exception to this rule is the start of the crawl process where it gets a task without an input bulk, which causes it to generate an input record from the task parameters or configuration in a later version.). Then the components are executed like this: ...
Many homeowners searching the internet for crawlspace repair information end up in home repair forums. While there is some good information out there, there are many examples of bad and outdated advice...
The iron will of Roger Federer and the burning desire of Novak Djokovic carried them into the quarter-finals of the US Open on Tuesday.
As bizarre as their migration might seem, there also are bizarre human stories surrounding these amazing worms. In 1922, Shimesu Koino ate 2000 eggs of Ascaris lumbricoides, and fed 500 Acaris suum eggs from pigs to his younger brother! Koino didnt fare so well, but he did demonstrate the pulmonary syndrome associated with early Ascaris infections. He also inferred the route of migration by finding larvae in his sputum. In 1970, annoyed that his roommates were bugging him to pay his rent on time, a postdoc at McGill University in Montreal, Eric Kranz, laced their food with hundreds of thousands of Ascaris lumbricoides eggs that he had taken from the parasitology lab at Macdonald College. Richard Davis, William Butler, David Fisk, and Keith Fern were each hospitalized and Kranz was charged with attempted murder. Ascaris eggs are extremely resistant to destruction; they remain viable for months to years. In 1920, Yoshido even demonstrated their ability to remain viable in the face of ...
Infections can help fight infertility. Stunned? Recently, the Journal Science confirmed a research that parasite Ascaris lumbricoides might be an additional boon for women who are trying to be pregnant for quite some time.
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Noi, o albino, A menina da fábrica de fósforos, the black Dahlia, há alguns interessantes, os dois primeiros mais difíceis de se encontrar. Pena que o makingoff.org não está mais ativo. Rafael Kafka Dubito ergo cogito, cogito ergo sum , Justitia est constans et perpetua voluntas jus suum cuique tribuendi , ******************KAFKA Extreme******************** , PENTIUM-D 930 *PRESLER* , Gigabyte 8I945GMF , X300 HM , 2x 512mb DDR2-667 Kingston Value , , LCD 17 Samsung 721s , Hitachi 160GB SATA II , X7-8600 7.1 , FreeBSD 6.1 , Gentoo GNU/Linux 2006.1 , GNU BIGLINUX 3.0 , Ubuntu GNU/Linux Dapper Drake 6.06.1 LTS , Novell SuSe GNU/Linux 10.1 X86_64 , Seja livre, use GNU/Linux! , GNU/Linux user: #393659 , Machine number #294871 , http://counter.li.org , Liberte-se! Use o Firefox., Omar Kaminski escreveu: , Sei lá ...
Revolution is the first feline topical roundworm treatment approved by the U.S. Food and Drug Administration. Selamectin, its active ingredient, is responsible for its action against roundworms and ...
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All multicellular organisms that reproduce sexually rely on eggs to support early life. Researchers at University of California, San Diego School of Medicine and Ludwig Cancer Research used the tiny roundworm C. elegans as ...
Roundworm infection is a type of parasitic illness. This is an illness in which an organism lives inside the body of another creature. The worms live and grow inside the body and may cause symptoms.
Reader Question: How to Treat Dog Roundworms We have a six week old puppy we took today and had its first shots and wormed. Tonight she has a bit of diarrhea
This was written by joannemerriam. Posted on Thursday, September 10, 2009, at 1:42 pm. Filed under People I Know, Poetry. Bookmark the permalink. Follow comments here with the RSS feed. Both comments and trackbacks are currently closed ...
Got up but found no D.C. We looked high and low, in and out, no D.C. Went into my husbands office and noticed the closet door was open and looked inside... the boy had pried open the crawl space entrance! So now we knew how he was getting out but we had to find him. Poor Burton crawled under the house and found him at the far end trying to get one of the crawl space windows (that he had already opened once) back open. D.C. refused to come when called (because, of course, he knew he had done something wrong) so B man had to drag his sorry self through the dirt all the way under the house to get him out. All this before 7am. There was rat poison under there and we didnt know if DC had eaten any ...
The Internet Archive discovers and captures web pages through many different web crawls. At any given time several distinct crawls are running, some for months, and some every day or longer. View the web archive through the Wayback Machine ...
The Internet Archive discovers and captures web pages through many different web crawls. At any given time several distinct crawls are running, some for months, and some every day or longer. View the web archive through the Wayback Machine ...
Writing that title alone gave me heart trouble. It was hairy there for a minute, I almost Michael Jacksoned on you. Anyway, more robotic death. Remember: what doesnt kill you makes you stronger. Unfortunately, these will kill you. Researchers...
Being a working mom does have some downfalls, ok several. One of them being that there is a chance that I will miss milestones in person. Thanks to technology however, I get to experience them via video, as do all of my co-workers.. While at work, Rick sent me a video of Noah pulling to stand for the first time. Who knew he had this in him? The last milestone Noah hit was the inch worm crawl, so we werent even on the look out for Noah to pull himself up on his own right now.. [Read More…] ...
On your mark. Get set. Grow! Our get-huge hit list below can make your race for muscle size a record-setting sprint rather than a slow crawl. Weve learned a few things about getting bigger and share them right here!
Long blue notes echo in my soul. Whole?No. The blue notes invademy soul, metastasize like rock hardtumors. Cancerous cells move glacier-like along the line of my spine, tillsitting up straight feels like balancingthe whole world on my back. Thosenotes carry me homewhere I crawl into bed, wait to die. Exhale my…
I have a mid-2007 24-inch iMac (2.8GHz Core2 Duo with 4GB RAM and 256MB graphics). Unsurprisingly, it crawls to a halt when I work with the large...
If there is one company that makes my skin crawl, its Crapna. (Sony, how could you screw your employees this way?) Im sure that they have a better plan than I do, but in general Crapna is exactly what I said...crapna. You have to take my word on this. Anyone on the other side of…
As long as you're out there, they’re out there; your mini explorer wants to see it all, too. Let your little one crawl around in this warm full-zip hoodie that's crafted of durable midweight fleece.
In the last couple of weeks we have seen quite a lot of change in our infant. She is finally getting up on her knees to crawl instead of doing her
The symptoms of the bizarre illness known as Morgellons are enough to make your skin crawl. For patients who say they are suffering from the condition, that sensation is all too real.. ...
Ascaris suum, pig-infecting giant roundworm, closely related to human-infecting giant roundworm Ascaris lumbricoides (2011) ... October 26, 2011). "Ascaris suum draft genome". Nature. 479 (7374): 529-533. Bibcode:2011Natur.479..529J. doi:10.1038/ ...
Ascaris suum) Mollusca • Owl limpet (Lottia gigantea) • Pacific oyster (Crassostrea gigas) Annelida • Polychaete worm ( ...
In pigs the infection is due to Ascaris suum. In horses and other equines, the equine roundworm is Parascaris equorum and the ... Ascaris lumbricoides adult worms Ascaris egg, incubation process: The Ascaris egg incubation process consists in placing the ... Prophylactic activity in a mouse-ascaris suum test model". J. Parasitol. The Journal of Parasitology, Vol. 57, No. 3. 57 (3): ... The larva of Ascaris lumbricoides developing in the egg Ascaris lumbricoides adult worms (with measuring tape for scale) ...
Muller, S.; Walter, R.D. (1992). "Purification and characterization of polyamine oxidase from Ascaris suum". Biochem. J. 283: ...
Morphine increases the latency of this defensive response in the parasitic Ascaris suum. In a study on the effects of opiates ... The effect of opiates and opiate antagonists on heat latency response in the parasitic nematode Ascaris suum. Life Sciences, 80 ...
In Ascaris suum, two MSP-fiber proteins (MFP), MFP1 and 2, with opposite effect on polymerization have been identified. MFP1 ... Molecular structures of MSP from Ascaris suum and Caenorhabditis elegans have been determined by X-ray crystallography and NMR ... of Ascaris suum". J. Mol. Biol. 263 (2): 284-96. doi:10.1006/jmbi.1996.0575. PMID 8913307. Haaf A, LeClaire L, Roberts G, Kent ... of Ascaris suum - evidence for two manganese binding sites and the possible role of divalent cations in filament formation". J ...
Roepstorff A, Murrell KD (May 1997). "Transmission dynamics of helminth parasites of pigs on continuous pasture: Ascaris suum ...
A possible mechanism for the action of the novel anthelmintic emodepside, using Ascaris suum body wall muscle preparations - ... "The effect of the anthelmintic emodepside at the neuromuscular junction of the parasitic nematode Ascaris suum". Parasitology. ... inhibiting muscle in the parasitic nematode Ascaris sum and inhibiting locomotive and pharyngeal movement in Caenorhabditis ...
In its native habitat, this species preys on eggs of the parasite Ascaris suum, which survive and develop after passage through ... The role of mollusks as benthos components in purification of water bodies from Ascaris suum eggs. Paraziologiya 9(5):432-433. ...
"The effect of opiates and opiate antagonists on heat latency response in the parasitic nematode Ascaris suum". Life Sciences. ...
In some eukaryotes, such as the parasitic worm Ascaris suum, an enzyme similar to complex II, fumarate reductase (menaquinol: ... "Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum". Biochim ...
"Crystal Structure of the Malic Enzyme from Ascaris suum Complexed with Nicotinamide Adenine Dinucleotide at 2.3 Å Resolution". ...
... identification of extracts with potent activity against Ascaris suum in vitro". Parasite. 23: 24. doi:10.1051/parasite/2016024 ... showed than ethanolic extracts of Clausena anisata have in vitro anthelmintic properties against the nematode Ascaris suum, a ...
... P1 Peptide Sequence (SWLSKTAKKLENSAKKRISEGIAIAIQGGPR). An antibacterial peptide from Ascaris suum, a parasitic ...
... zanthoxyloides have in vitro anthelmintic properties against the nematode Ascaris suum, a swine parasite that is closely ... identification of extracts with potent activity against Ascaris suum in vitro". Parasite. 23: 24. doi:10.1051/parasite/2016024 ...
Komuniecki R, McCrury J, Thissen J, Rubin N (1989). "Electron-transfer flavoprotein from anaerobic Ascaris suum mitochondria ...
... and initiation factors to nematode translation in an Ascaris suum embryo cell-free system". J. Biol. Chem. 279 (44): 45573-85. ...
Pada beberapa eukariota seperti cacing parasit Ascaris suum, terdapat enzim yang mirip dengan kompleks II, yaitu fumarat ... "Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum". Biochim ...
... of the genus Ascaris (giant intestinal roundworms). The large roundworm of pigs (Ascaris suum) typically infects pigs while ... Piperazine, a flaccid paralyzing agent that causes a blocking response of ascaris muscle to acetylcholine. The narcotizing ... Ascaris lumbricoides affects human populations, typically in sub-tropical and tropical areas with poor sanitation. Ascaricides ...
... these isoforms are also found in Ascaris suum and Caenorhabditis elegans. The subunits form a membrane-bound cytochrome b ...
Ascaris suum". J Biol Chem. 260: 4770-4777. PMID 3988734. Kionka, C.; Kunau, W.H. (1985). "Inducible β-oxidation pathway in ...
U niektórych eukariotów, takich jak należąca do pasożytniczych nicieni Ascaris suum, enzym zbliżony do kompleksu II, reduktaza ... Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum. „Biochim ...
... ascaris lumbricoides MeSH B01.500.500.294.700.100.100.108.700 --- ascaris suum MeSH B01.500.500.294.700.100.100.700 --- ...
Homologous sequences were found in species as distantly related to humans as the pig roundworm Ascaris suum, from the family ...
... including the nematodes such as Ascaris suum, Ascaris lumbricoides, Ascaridia galli, Heterakis gallinarum, a cestode ...
Ascaris suum. *Dicrocoelium dendriticum. *Dictyocaulus viviparus. *Dipylidium caninum. *Echinococcus granulosus. *Fasciola ...
Venn diagram showing the numbers of homologs between Haemonchus contortus and four other nematode species (Ascaris suum, Brugia ... and Ascaris suum [12-14]. We identified 40,046 retrotransposon sequences (see Additional file 1, Table S2) representing at ... Homologs between H. contortus and A. suum, B. malayi, C. elegans, and T. spiralis were inferred by comparison of all proteins ... suum [14], B. malayi [15]. C. elegans [116], and T. spiralis [16]. In the final annotation, proteins inferred from genes were ...

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