A species of parasitic nematode usually found in domestic pigs and a few other animals. Human infection can also occur, presumably as result of handling pig manure, and can lead to intestinal obstruction.
A genus of nematodes of the superfamily ASCARIDOIDEA whose species usually inhabit the intestine.
Infection by nematodes of the genus ASCARIS. Ingestion of infective eggs causes diarrhea and pneumonitis. Its distribution is more prevalent in areas of poor sanitation and where human feces are used for fertilizer.
A species of parasitic nematode that is the largest found in the human intestine. Its distribution is worldwide, but it is more prevalent in areas of poor sanitation. Human infection with A. lumbricoides is acquired by swallowing fully embryonated eggs from contaminated soil.
Proteins found in any species of helminth.
Analogs or derivatives of bephenium (N,N-dimethyl-N-(2-phenoxyethyl)benzenemethanaminium).
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
Used formerly as antimicrobial food additive. It causes mutations in many cell cultures and may be carcinogenic.
Agents destructive to parasitic worms. They are used therapeutically in the treatment of HELMINTHIASIS in man and animal.
Deoxyribonucleic acid that makes up the genetic material of helminths.
Substances used in the treatment or control of nematode infestations. They are used also in veterinary practice.
A condition produced in man by the prolonged migration of animal nematode larvae in extraintestinal tissues other than skin; characterized by persistent hypereosinophilia, hepatomegaly, and frequently pneumonitis, commonly caused by Toxocara canis and Toxocara cati.
An antihelminthic drug that has been tried experimentally in rheumatic disorders where it apparently restores the immune response by increasing macrophage chemotaxis and T-lymphocyte function. Paradoxically, this immune enhancement appears to be beneficial in rheumatoid arthritis where dermatitis, leukopenia, and thrombocytopenia, and nausea and vomiting have been reported as side effects. (From Smith and Reynard, Textbook of Pharmacology, 1991, p435-6)
Immunoglobulins produced in a response to HELMINTH ANTIGENS.
A species of parasitic nematode found in the intestine of dogs. Lesions in the brain, liver, eye, kidney, and lung are caused by migrating larvae. In humans, these larvae do not follow normal patterns and may produce visceral larva migrans (LARVA MIGRANS, VISCERAL).
Ribonucleic acid in helminths having regulatory and catalytic roles as well as involvement in protein synthesis.
A plant genus of the family OLEACEAE. Members contain secoiridoid glucosides.
The span of viability of a tissue or an organ.
Monitoring of rate of occurrence of specific conditions to assess the stability or change in health levels of a population. It is also the study of disease rates in a specific cohort such as in a geographic area or population subgroup to estimate trends in a larger population. (From Last, Dictionary of Epidemiology, 2d ed)
The profession of writing. Also the identity of the writer as the creator of a literary production.
Educational institutions providing facilities for teaching and research and authorized to grant academic degrees.
Organized activities related to the storage, location, search, and retrieval of information.
Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
The ability of fungi to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance phenotype may be attributed to multiple gene mutations.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Libraries in which a major proportion of the resources are available in machine-readable format, rather than on paper or MICROFORM.
The rate dynamics in chemical or physical systems.
An enzyme that catalyzes the conversion of (S)-malate and NAD+ to oxaloacetate and NADH. EC 1.1.1.37.
A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)
An enzyme that catalyzes the conversion of alpha,alpha-trehalose and water to D-glucose. EC 3.2.1.28.
The fertilized OVUM resulting from the fusion of a male and a female gamete.
A water-soluble, colorless crystal with an acid taste that is used as a chemical intermediate, in medicine, the manufacture of lacquers, and to make perfume esters. It is also used in foods as a sequestrant, buffer, and a neutralizing agent. (Hawley's Condensed Chemical Dictionary, 12th ed, p1099; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1851)
Proteins obtained from ESCHERICHIA COLI.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A genus of ascarid nematodes commonly parasitic in the intestines of cats and dogs.
Infection by round worms of the genus TOXOCARA, usually found in wild and domesticated cats and dogs and foxes, except for the larvae, which may produce visceral and ocular larva migrans in man.
Skin tests in which the sensitizer is injected.
Epicutaneous or intradermal application of a sensitizer for demonstration of either delayed or immediate hypersensitivity. Used in diagnosis of hypersensitivity or as a test for cellular immunity.
Proteins prepared by recombinant DNA technology.
A publication issued at stated, more or less regular, intervals.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
A plant genus of the family FABACEAE that is the source of mucuna gum.
A plant family of the order Sapindales, subclass Rosidae, class Magnoliopsida. They are resinous trees and shrubs with alternate leaves composed of many leaflets.
A genus of parasitic nematode worms which infest the duodenum and stomach of domestic and wild herbivores, which ingest it with the grasses (POACEAE) they eat. Infestation of man is accidental.
The common chimpanzee, a species of the genus Pan, family HOMINIDAE. It lives in Africa, primarily in the tropical rainforests. There are a number of recognized subspecies.
A supergroup (some say phylum) of ameboid EUKARYOTES, comprising ARCHAMOEBAE; LOBOSEA; and MYCETOZOA.
DNA-binding motifs formed from two alpha-helixes which intertwine for about eight turns into a coiled coil and then bifurcate to form Y shaped structures. Leucines occurring in heptad repeats end up on the same sides of the helixes and are adjacent to each other in the stem of the Y (the "zipper" region). The DNA-binding residues are located in the bifurcated region of the Y.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Basic polypeptide from the venom of the honey bee (Apis mellifera). It contains 26 amino acids, has cytolytic properties, causes contracture of muscle, releases histamine, and disrupts surface tension, probably due to lysis of cell and mitochondrial membranes.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.

Nematode pyruvate dehydrogenase kinases: role of the C-terminus in binding to the dihydrolipoyl transacetylase core of the pyruvate dehydrogenase complex. (1/169)

Pyruvate dehydrogenase kinases (PDKs) from the anaerobic parasitic nematode Ascaris suum and the free-living nematode Caenorhabditis elegans were functionally expressed with hexahistidine tags at their N-termini and purified to apparent homogeneity. Both recombinant PDKs (rPDKs) were dimers, were not autophosphorylated and exhibited similar specific activities with the A. suum pyruvate dehydrogenase (E1) as substrate. In addition, the activities of both PDKs were activated by incubation with PDK-depleted A. suum muscle pyruvate dehydrogenase complex (PDC) and were stimulated by NADH and acetyl-CoA. However, the recombinant A. suum PDK (rAPDK) required higher NADH/NAD+ ratios for half-maximal stimulation than the recombinant C. elegans PDK (rCPDK) or values reported for mammalian PDKs, as might be predicted by the more reduced microaerobic mitochondrial environment of the APDK. Limited tryptic digestion of both rPDKs yielded stable fragments truncated at the C-termini (trPDKs). The trPDKs retained their dimeric structure and exhibited substantial PDK activity with the A. suum E1 as substrate, but PDK activity was not activated by incubation with PDK-depleted A. suum PDC or stimulated by elevated NADH/NAD+ or acetyl-CoA/CoA ratios. Direct-binding assays demonstrated that increasing amounts of rCPDK bound to the A. suum PDK-depleted PDC. No additional rCPDK binding was observed at ratios greater than 20 mol of rCPDK/mol of PDC. In contrast, the truncated rCPDK (trCPDK) did not exhibit significant binding to the PDC. Similarly, a truncated form of rCPDK, rCPDK1-334, generated by mutagenesis, exhibited properties similar to those observed for trCPDK. These results suggest that the C-terminus of the PDK is not required for subunit association of the homodimer or catalysis, but instead seems to be involved in the binding of the PDKs to the dihydrolipoyl transacetylase core of the complex.  (+info)

Developmentally regulated telomerase activity is correlated with chromosomal healing during chromatin diminution in Ascaris suum. (2/169)

Telomerase is the ribonucleoprotein complex responsible for the maintenance of the physical ends, or telomeres, of most eukaryotic chromosomes. In this study, telomerase activity has been identified in cell extracts from the nematode Ascaris suum. This parasitic nematode is particularly suited as a model system for the study of telomerase, because it shows the phenomenon of chromatin diminution, consisting of developmentally programmed chromosomal breakage, DNA elimination, and new telomere formation. In vitro, the A. suum telomerase is capable of efficiently recognizing and elongating nontelomeric primers with nematode-specific telomere repeats by using limited homology at the 3' end of the DNA to anneal with the putative telomerase RNA template. The activity of this enzyme is developmentally regulated, and it correlates temporally with the phenomenon of chromatin diminution. It is up-regulated during the first two rounds of embryonic cell divisions, to reach a peak in 4-cell-stage embryos, when three presomatic blastomeres prepare for chromatin diminution. The activity remains high until the beginning of gastrulation, when the last of the presomatic cells undergoes chromatin diminution, and then constantly decreases during further development. In summary, our data strongly argue for a role of this enzyme in chromosome healing during the process of chromatin diminution.  (+info)

Sequence-divergent units of the ABA-1 polyprotein array of the nematode Ascaris suum have similar fatty-acid- and retinol-binding properties but different binding-site environments. (3/169)

Polyproteins comprise long polypeptides that are post-translationally cleaved into proteins of different function, or tandemly repetitive polypeptides which are processed into multiple versions of proteins which are presumed to have the same function. In the latter case the individual units of the polyprotein can differ substantially in sequence. Identity of function between the different units therefore cannot be assumed. Here we have examined the ABA-1 polyprotein allergen of the parasitic nematode Ascaris suum and found it to contain units which show a 50% difference in amino acid sequence. The parasite therefore produces at least two radically different forms of the allergen encoded within the polyprotein array. In fluorescence-based ligand-binding assays, recombinant polypeptides representing the two forms (designated ABA-1A1 and ABA-1B1) showed similar binding affinities for a range of fluorescent active-site probes [retinol, dansylundecanoic acid, dansyl-DL-alpha-amino-octanoic acid, cis-parinaric acid (cPnA)] and for the non-specific hydrophobic surface probe 8-anilinonaphthalene-1-sulphonic acid. However, the molecular environments in the active sites are markedly different, as indicated by disparate fluorescence emission peaks and intensities of bound probes. CD showed that the proteins have similar secondary structures but differ in susceptibility to chemical denaturation/unfolding by guanidinium chloride. Both retain a single conserved tryptophan residue in a characteristic non-polar environment, as revealed by extreme fluorescence blue shift. Thus the gross differences in sequence of the two proteins are not reflected in their ligand-binding specificities but in their binding-site environments.  (+info)

Effect of an orally active Th1/Th2 balance modulator, M50367, on IgE production, eosinophilia, and airway hyperresponsiveness in mice. (4/169)

We have found a novel anti-allergic agent, M50367, which suppresses IgE biosynthesis and eosinophil accumulation in vivo. In this study, we evaluated the ability of M50367 to modulate Th1/Th2 balance in Th2-background BALB/c mice and to inhibit airway hyperresponsiveness in a murine model of atopic asthma. Oral M50367 at 3-30 mg/kg/day exhibited 51 to 73% reduction of IL-4/IL-5 production and 2- to 5-fold augmentation of IFN-gamma production by Ag-stimulated cultured splenocytes of the mice sensitized with DNP-Ascaris. These alterations in Th1/Th2 cytokine production were accompanied by 55-85% suppression of plasma IgE level. Oral M50367 at a dose of 10 mg/kg/day significantly inhibited Ig-independent peritoneal eosinophilia by 54%, which was induced by repeated i.p. injections of Ascaris suum extract. To develop airway hyperresponsiveness caused by allergic airway inflammation, BALB/c mice were sensitized with i.p. OVA injections, followed three times by OVA inhalation. Oral M50367 significantly inhibited the increase in airway reactivity to acetylcholine, together with the elevation of plasma IgE level and pulmonary eosinophilia, which were observed in vehicle-treated mice 1 day after the last inhalation. Moreover, M50367 treatment reduced IL-4 and IL-5 production and tended to enhance IFN-gamma production, not only by cultured splenocytes, but also in bronchoalveolar lavage fluid. These results suggest that M50367 has a modulating ability of Th1/Th2 balance to down-regulate Th2 response in the circulating system as well as at the sites of inflammation, and may be beneficial for the treatment of allergic disorders such as atopic asthma.  (+info)

Localized depolymerization of the major sperm protein cytoskeleton correlates with the forward movement of the cell body in the amoeboid movement of nematode sperm. (5/169)

The major sperm protein (MSP)-based amoeboid motility of Ascaris suum sperm requires coordinated lamellipodial protrusion and cell body retraction. In these cells, protrusion and retraction are tightly coupled to the assembly and disassembly of the cytoskeleton at opposite ends of the lamellipodium. Although polymerization along the leading edge appears to drive protrusion, the behavior of sperm tethered to the substrate showed that an additional force is required to pull the cell body forward. To examine the mechanism of cell body movement, we used pH to uncouple cytoskeletal polymerization and depolymerization. In sperm treated with pH 6.75 buffer, protrusion of the leading edge slowed dramatically while both cytoskeletal disassembly at the base of the lamellipodium and cell body retraction continued. At pH 6.35, the cytoskeleton pulled away from the leading edge and receded through the lamellipodium as its disassembly at the cell body continued. The cytoskeleton disassembled rapidly and completely in cells treated at pH 5.5, but reformed when the cells were washed with physiological buffer. Cytoskeletal reassembly occurred at the lamellipodial margin and caused membrane protrusion, but the cell body did not move until the cytoskeleton was rebuilt and depolymerization resumed. These results indicate that cell body retraction is mediated by tension in the cytoskeleton, correlated with MSP depolymerization at the base of the lamellipodium.  (+info)

1H NMR investigation of the distal hydrogen bonding network and ligand tilt in the cyanomet complex of oxygen-avid Ascaris suum hemoglobin. (6/169)

The O(2)-avid hemoglobin from the parasitic nematode Ascaris suum exhibits one of the slowest known O(2) off rates. Solution (1)H NMR has been used to investigate the electronic and molecular structural properties of the active site for the cyano-met derivative of the recombinant first domain of this protein. Assignment of the heme, axial His, and majority of the residues in contact with the heme reveals a molecular structure that is the same as reported in the A. suum HbO(2) crystal structure (Yang, J., Kloek, A., Goldberg, D. E., and Mathews, F. S. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 4224-4228) with the exception that the heme in solution is rotated by 180 degrees about the alpha,gamma-meso axis relative to that in the crystal. The observed dipolar shifts, together with the crystal coordinates of HbO(2), provide the orientation of the magnetic axes in the molecular framework. The major magnetic axis, which correlates with the Fe-CN vector, is found oriented approximately 30 degrees away from the heme normal and indicates significant steric tilt because of interaction with Tyr(30)(B10). The three side chain labile protons for the distal residues Tyr(30)(B10) and Gln(64)(E7) were identified, and their relaxation, dipolar shifts, and nuclear Overhauser effects to adjacent residues used to place them in the distal pocket. It is shown that these two distal residues exhibit the same orientations ideal for H bonding to the ligand and to each other, as found in the A. suum HbO(2) crystal. It is concluded that the ligated cyanide participates in the same distal H bonding network as ligated O(2). The combination of the strong steric tilt of the bound cyanide and slow ring reorientation of the Tyr(30)(B10) side chain supports a crowded and constrained distal pocket.  (+info)

Effect of the injection of an extract of Ascaris suum on macrophage activation during the early phase of Mycobacterium bovis BCG infection in C57Bl/6 mice. (7/169)

Injection of an Ascaris suum extract (Asc) affects both the humoral and cellular immune responses to unrelated antigens when it is co-administered with these antigens. In the present study we evaluated the effect of Asc on macrophage activation in the early phase of Mycobacterium bovis BCG (Pasteur strain TMCC 1173) infection in C57Bl/6 mice. C57Bl/6 mice were injected intraperitoneally (ip) with 0.1 mg BCG (BCG group) or BCG plus 1 mg Asc (BCG + Asc group). The peritoneal exudates were obtained at 2, 7 and 14 days after infection. The numbers of IFN-gamma-secreting cells were assessed by the ELISPOT assay. Nitric oxide (NO) production was measured by the Griess method and by the evaluation of NADPH diaphorase activity in the peritoneal exudates. The administration of Asc extract increased NADPH diaphorase activity (2 days: control = 0, BCG = 7%, BCG + Asc = 13%, and Asc = 4%; 7 days: control = 4, BCG = 13%, BCG + Asc = 21%, and Asc = 4.5%) and TNF-alpha levels (mean +/- SD; 2 days: control = 0, BCG = 169 +/- 13, BCG + Asc = 202 +/- 37, and Asc = 0; 7 days: control = 0, BCG = 545 +/- 15.5, BCG + Asc = 2206 +/- 160.6, and Asc = 126 +/- 26; 14 days: control = 10 +/- 1.45, BCG = 9 +/- 1.15, BCG + Asc = 126 +/- 18, and Asc = 880 +/- 47.67 pg/ml) in the early phase of BCG infection. Low levels of NO production were detected at 2 and 7 days after BCG infection, increasing at 14 days (mean +/- SD; 2 days: control = 0, BCG = 3.7 +/- 1.59, BCG + Asc = 0.82 +/- 0.005, Asc = 0.48 +/- 0.33; 7 days: control = 0, BCG = 2.78 +/- 1.54, BCG + Asc = 3.07 +/- 1.05, Asc = 0; 14 days: control = 0, BCG = 9.05 +/- 0.53, BCG + Asc = 9.61 +/- 0.81, Asc = 10.5 +/- 0.2 (2 x 10(6)) cells/ml). Furthermore, we also observed that Asc co-injection induced a decrease of BCG-colony-forming units (CFU) in the spleens of BCG-infected mice during the first week of infection (mean +/- SD; 2 days: BCG = 1.13 +/- 0.07 and BCG + Asc = 0.798 +/- 0.305; 7 days: BCG = 1.375 +/- 0. 194 and BCG + Asc = 0.548 +/- 0.0226; 14 days: BCG = 0.473 +/- 0.184 and BCG + Asc = 0.675 +/- 0.065 (x 10(2)) CFU). The present data suggest that Asc induces the enhancement of the immune response in the early phase of BCG infection.  (+info)

Phosphocholine-containing, zwitterionic glycosphingolipids of adult Onchocerca volvulus as highly conserved antigenic structures of parasitic nematodes. (8/169)

Human Onchocerca volvulus infection sera were found to recognize zwitterionic glycolipids of O. volvulus and to cross-react with those of other parasitic nematodes (Ascaris suum, Setaria digitata and Litomosoides sigmodontis). By the use of an epitope-specific monoclonal antibody, zwitterionic glycolipids of all these nematode species were observed to contain the antigenic determinant phosphocholine. A hyperimmune serum specific for arthro-series glycolipid structures reacted with the various neutral glycolipids of all these nematodes, which demonstrated that their oligosaccharide moieties belonged to the arthro-series of protostomial glycolipids. These results indicated that arthro-series glycosphingolipids carrying, in part, phosphocholine substituents, represent highly conserved, antigenic glycolipid markers of parasitic nematodes. Three glycolipid components of the O. volvulus zwitterionic fraction were structurally characterized by matrix-assisted laser-desorption/ionization time-of-flight MS, methylation analysis and exoglycosidase treatment. Their chemical structures were elucidated to be phosphocholine-6GlcNAc(beta1-3)Man(beta1-4)Glc(1-1)ceramide, GalNAc(beta1-4)[phosphocholine-6]GlcNAc(beta1-3)Man(beta1-4)Glc(1-1) ceramide and Gal(alpha1-3)GalNAc(beta1-4)[phosphocholine-6]GlcNAc(beta1-3)Man(beta 1-4)Glc(1-1)ceramide for the zwitterionic ceramide tri-, tetra- and penta-hexosides respectively. The ceramide composition was found to be dominated by 2-hydroxylated docosanoic (C(22h:0)), tricosanoic (C(23h:0)) and tetracosanoic (C(24h:0)) acids, and C(17) sphingosine (C(d17:1)) (where (h) is hydroxylated and (d) is dihydroxylated).  (+info)

BACKGROUND: Caenorhabditis elegans has traditionally been used as a model for studying nematode biology, but its small size limits the ability for researchers to perform some experiments such as high-throughput tissue-specific gene expression studies. However, the dissection of individual tissues is possible in the parasitic nematode Ascaris suum due to its relatively large size. Here, we take advantage of the recent genome sequencing of Ascaris suum and the ability to physically dissect its separate tissues to produce a wide-scale tissue-specific nematode RNA-seq datasets, including data on three non-reproductive tissues (head, pharynx, and intestine) in both male and female worms, as well as four reproductive tissues (testis, seminal vesicle, ovary, and uterus). We obtained fundamental information about the biology of diverse cell types and potential interactions among tissues within this multicellular organism. METHODOLOGY/PRINCIPAL FINDINGS: Overexpression and functional enrichment analyses ...
Williamson, S. M., Robertson, A. P., Brown, L., Williams, T., Woods, D. J., Martin, R. J., Sattelle, D. B. and Wolstenholme, A., 2009. The nicotinic acetylcholine receptors of the parasitic nematode ascaris suum: formation of two distinct drug targets by varying the relative expression levels of two subunits. PLoS Pathogens ...
The levels of trehalose and the activity of trehalase during the development of Ascaris suum eggs were investigated. The level of trehalose in the zygote was high (2.96 ± 0.07 mg/g). During cleavage of eggs, it decreased (0.91 ± 0.35 mg/g). A higher concentration of the sugar was recorded at the blastula and during gastrulation, but it did not reach the uncleaved eggs level. In the early motile larvae, the concentration of trehalose was high (4.58 ± 2.01 mg/g). It decreased with development of L1 larvae (3.10 ± 1.47 mg/g). A rapid increase in trehalose reserves was observed between the L1 and L2 stages. The highest content of trehalose was found in invasive L2 larvae (5.78 ± 1.39 mg/g).. The activity of trehalase at the zygote stage was high (560.22 ± 322.31 U/mg). It decreased at the beginning of cleavage. It was the lowest at the 4-6-cell stage (189.76 ± 114.97 U/mg). An increase in the enzyme activity occurred after reaching the blastula stage (348.44 ± 343.34 U/mg). The highest ...
A procedure for the isolation of malic enzyme from muscle tissue of the roundworm Ascaris suum is described. The fractionation method yields relatively large quantities of the enzyme,with a specific activity of fifteen moles of malate converted to pyruvate and carbon dioxide per min per mg at 25º. Homogeneity was established with analytical ultracentrifugation, zone electrophoresis, isoelectric focusing, and rechromatography. The molecular weight of the enzyme was 250,000, and it is dissociated under several conditions into four identical monomers of 64,000 daltons. The enzyme exists as a single electrophoretic form and prefers manganous and NAD over other cations and NADP. Ammonium sulfate competes with manganous for the active site and titration with DTNB yields eight thiol groups per mole. Titration of the first four thiol groups is accompanied by a complete loss in enzyme activity. Equilibrium dialysis, product inhibition, and initial velocity studies suggest a rapid-equilibrium random sequential
Read Protective immunity elicited by the nematode-conserved As37 recombinant protein against Ascaris suum infection, PLoS Neglected Tropical Diseases on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
O setor suinícola brasileiro tem enfrentado nos últimos anos, barreiras para exportação da carne, principalmente relacionadas a questões sanitárias e ao bem-estar animal. Em relação às questões sanitárias, grande destaque tem sido dado às parasitoses intestinais causadas por helmintos e às falhas no processo de vermifugação decorrentes da resistência a antiparasitários. Portanto, é crescente a necessidade de se estudar o perfil epidemiológico dos parasitas que acometem os animais e a partir daí avaliar estratégias de controle alternativos e sustentáveis.O objetivo desse trabalho foi avaliar métodos alternativos de controle para Ascaris suum na produção de suínos. O trabalho foi dividido em quatro estudos, realizados em etapas distintas. No primeiro objetivou-se avaliar a epidemiologia do A. suum em rebanhos suinícolas localizado na micro-região de Ponte Nova, na Zona da Mata de Minas Gerais - Brasil. No segundo ensaio, foi avaliada a constituição fitoquímica do ...
TY - JOUR. T1 - Functional depression of airway H2-histamine receptors in sheep allergic to Ascaris suum antigen. AU - Krainson, J.. AU - Yerger, L.. AU - Ahmed, T.. PY - 1981/1/1. Y1 - 1981/1/1. UR - http://www.scopus.com/inward/record.url?scp=0019453567&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0019453567&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0019453567. VL - 123. JO - American Journal of Respiratory and Critical Care Medicine. JF - American Journal of Respiratory and Critical Care Medicine. SN - 1073-449X. IS - 4 II. ER - ...
Abstract: Ascaris suum extract (Asc) down-regulates the immune response to ovalbumin (OA) as well as preferentially stimulates a Th2 response to its own components, which is responsible for the suppression of the OA-specific Th1 response. IL-4 plus IL-10-specific monoclonal antibody treatment of mice before immunization with OA + Asc abolishes this suppression. The immunosuppressive activity of Asc is due to high MW proteins (PI) that are obtained by gel filtration chromatography. Although PI stimulates IL-4 and IL-10 secretion, IgE and anaphylactic IgG1 antibodies against PI are only obtained in IL-12 or IFN-g-/-deprived mice. In this work, we investigated the role of IL-12 and IFN-g in the suppression exerted by PI on an unrelated immune response. IFN-g- or IL-12-deficient (IL-12-/- / IFN-g-/-) or wild-type mice were immunized with OA or OA + PI in complete Freunds adjuvant and challenged in the hind footpad with aggregated OA after 8 days. Immediate and delayed-type-hypersensitivity (DTH) ...
The disulphide bond arrangement in the major pepsin inhibitor PI-3 of Ascaris suum. FEBS Letters 474 (2-3) , pp. 253-255. 10.1016/S0014-5793(00)01589-1 ...
The malic enzyme gene of Ascaris suum was cloned into the vector pTRC99a in two forms encoding alternative amino-termini. The resulting plasmids, pMEA1 and pMEA2, were introduced into Escherichia coli...
Google Scholar Page. Peer reviewed papers since 2014. ** Denotes a student that Dr. Karriker mentored, served as major professor, served as graduate committee member, or served as professional (DVM) curriculum advisor during the study.. Pittman J**, Myers G, Stalder K, Karriker L. 2015. Effect of fenbendazole on shedding and embryonation of Ascaris suum eggs from naturally infected sows. Journal of Swine Health and Production. 23(5):252-263.. Day D**, Sparks J**, Karriker L, Stalder K, Wulf L, Zhang J, Kinyon J, Stock M, Gehring R, Wang C, Ellingson J, Coetzee J. 2015. Impact of an experimental PRRSV and Streptococcus suis coinfection on the pharmacokinetics of ceftiofur hydrochloride after intramuscular injection in pigs. Journal of Veterinary Pharmacology and Therapeutics. doi: 10.1111/jvp.12209. [Epub ahead of print].. Thomas P**, Karriker L, Ramirez A, Zhang J, Ellingson J, Crawford K**, Bates J**, Hammen K, Holtkamp D. 2015. Evaluation of time and temperature sufficient to inactivate ...
Staff publications is the digital repository of Wageningen University & Research. Staff publications contains references to publications authored by Wageningen University staff from 1976 onward.. Publications authored by the staff of the Research Institutes are available from 1995 onwards.. Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.. We have a manual that explains all the features ...
Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
WormBase ParaSite is an open access resource providing genome sequences, genome browsers, semi-automatic annotation and comparative genomics analysis for nematode and platyhelminth parasites (helminths).
WormBase ParaSite is an open access resource providing genome sequences, genome browsers, semi-automatic annotation and comparative genomics analysis for nematode and platyhelminth parasites (helminths).
Abstract The specificity and sensitivity of adult and larval somatic antigens, and perienteric fluid of Toxocara canis and Ascaris suum were investigated by using intradermal skin tests in guinea pigs. These animals were infected with low doses (0.01, 0.1 or 1.0 egg/g) of these helminths. Toxocara larval antigen (TL) induced larger reactions in Toxocara-infected animals than did the other antigens, suggesting a superior sensitivity for this antigen. In addition, Ascaris perienteric fluid (AP) provoked skin responses in these animals of a magnitude similar to those induced by TL. The reciprocal relationship, i.e., comparable reactivity of AP and TL in Ascaris-infected animals, was not apparent. In general, Ascaris larval antigen and AP elicited larger intradermal reactions than other antigens in Ascaris-infected animals. The results of this study indicated no superiority of adult antigens in differentiating Ascaris and Toxocara infections.
Sentinel Chambers are loaded with 100,000 Ascaris eggs and wastewater or biosolids taken directly from the treatment plant to be tested. Fine mesh screens on the ends of the Sentinel Chamber hold the eggs within the chamber and at the same time allow the interior to equilibrate with the surrounding environment. After the Sentinel Chamber is in the test environment for the requested period, the eggs are easily recovered to determine viability following disinfection ...
TY - JOUR. T1 - Dephosphorylation of major sperm protein (MSP) fiber protein 3 by protein phosphatase 2A during cell body retraction in the MSP-based amoeboid motility of Ascaris sperm. AU - Yi, Kexi. AU - Wang, Xu. AU - Emmett, Mark. AU - Marshall, Alan G.. AU - Stewart, Murray. AU - Roberts, Thomas M.. PY - 2009/7/15. Y1 - 2009/7/15. N2 - The crawling movement of nematode sperm requires coordination of leading edge protrusion with cell body retraction, both of which are powered by modulation of a cytoskeleton based on major sperm protein (MSP) filaments. We used a cell-free in vitro motility system in which both protrusion and retraction can be reconstituted, to identify two proteins involved in cell body retraction. Pharmacological and depletion-add back assays showed that retraction was triggered by a putative protein phosphatase 2A (PP2A, a Ser/Thr phosphatase activated by tyrosine dephosphorylation). Immunofluorescence showed that PP2A was present in the cell body and was concentrated at ...
TY - JOUR. T1 - Inhibition of allergic airway responses by inhaled low-molecular-weight heparins. T2 - Molecular-weight dependence. AU - Martinez-Salas, José. AU - Mendelssohn, Richard. AU - Abraham, William M.. AU - Hsiao, Bernard. AU - Ahmed, Tahir. PY - 1998/1. Y1 - 1998/1. N2 - Inhaled heparin prevents antigent-induced bronchoconstriction and inhibits anti-immunoglobulin E-mediated mast cell degranulation. We hypothesized that the antiallergic action of heparin may be molecular weight dependent. Therefore, we studied the effects of three different low- molecular-weight fractions of heparin [medium-, low-, and ultralow-molecular- weight heparin (MMWH, LMWH, ULMWH, respectively)] on the antigen-induced acute bronchoconstrictor response (ABR) and airway hyperresponsiveness (AHR) in allergic sheep. Specific lung resistance was measured in 22 sheep before and after airway challenge with Ascaris suum antigen, without and after pretreatment with inhaled fractionated heparins at doses of 0.31-5.0 ...
For many people in the west, a nematode infestation is an unpleasant, but rare event. Unfortunately, the same cannot be said for the inhabitants of many less developed countries, where nematodes are rife and cause many crippling diseases. Fortunately, modern medicine is equipped with a battery of drugs to rid patients of unwanted parasites. But the threat remains that the nematodes will eventually outsmart the pharmacologists and become resistant to the three major classes of drug currently in use. Which is why Richard Martin is keen to understand how one of these nematode targeting drugs, levamisole, attacks the nematode, Ascaris suum. He hopes this will help us to design new therapies to keep medicine ahead of the nematode in the race to beat resistance (p. 3979).. Martin explains that many of the drugs, called anthelmic drugs, which are currently so effective in treating roundworm infections, were originally developed for use in western veterinary practice. He explains that many of these ...
Both the Tsimane Amazonian Panel Study[6] and The Tsimane Health and Life History Project have studied the Tsimane since 2002.[7] Among other things, it appears that they do not develop heart disease as they age in the same ways as people in the developed world.[8] [9] The average Tsimané woman has nine children in her lifetime. A study of 983 Tsimané women found that 70% were infected with the parasitic roundworm Ascaris lumbricoides, which is believed to have increased their fertility rate by suppressing their immune system, leading to two additional children over the course of a lifetime.[10] ...
Looking for the best drainage solution for a crawl space foundation? Learn more about SmartPipe and other waterproofing options from Basement Systems that can help you keep your crawl space dry.
PubCrawls.com presents the annual New York Halloweekend Pub Crawl. NYC Halloweekend Bar Crawl Info, Tickets, Photos and more. Call 888-998-6609
PubCrawls.com presents the annual NYC Cinco de Mayo Pub Crawl. Cinco de Mayo Bar Crawl Info, Tickets, and more. Call 888-998-6609
Tjernlund Products is your source for crawl space ventilation. We carry products ranging from a room to room fan, dryer booster fan, a chimney fan, and more. Don�t worry about damp crawl space moisture again with our dryer boosting fan.
We will evaluate and develop a solution that will keep your crawl space dry, sealing the interior/exterior foundation with a lifetime water barrier membrane.
Posted by Zenae Zukowski on March 7, 2017 Towards the beginning of this year, we heard Anthrax were releasing their own blend of pale ale beer called Wardance. The NYC band will be celebrating the release of the brew with a beer crawl around the city on Wardances official release date Tuesday, March 28. The beer crawl will begin at 6:00 PM at […]. ...
Wet n Wild Crafty Kitten Crawl the Line Kit - A cat is nothing without its spots, with the Wet n Wild Crafty Kitten Crawl the Line Kit you can release your inner feline. This palette features 6 gl
Question - Child born premature not gaining required weight. Unable to sit and crawl. Having solids and sleeping properly . Ask a Doctor about diagnosis, treatment and medication for Weight loss, Ask a Pediatrician
Read the original post The 3 Best Crawl Space Dehumidifiers Reviews (Stop Mold, Mildew, and Bacteria Growth) on BabyDotDot - Baby Guide For Awesome Parents & More. You have taken care of all parts of the house, inside and outside. But there is a space underneath the floors or roof that is not always in…
I am delighted to organize events for Art Crawl. Borrowing from Kerrys original work, the goal of the group is to bring together people who want to attend creative, inspired events with like minded i
There are two pub crawl choices, operating seven evenings each week. One is into the Red Light, region beginning at Players club in Warmoes Street (click on this link to begin to see the cost). Be there between 8 and 9h20pm. Fair caution: that isnt a pickup club at all. One other, nicer pub crawl choice is during the Leidseplein (starting at Candela Bar between 8h20pm and 9h45pm). Youll visit a couple of bars that are touristic. It is maybe maybe not the pub crawl that is best, but nonetheless worthwhile if you think annoyed or alone through the night. The online pricing is exactly like the cost they charge on a lawn in Amsterdam. Click on through to GetYourGuide to reserve your spot. The last choice could be my very own personal option if it had been my first-time in Amsterdam: Get both pub crawls together for an extremely price that is nice. Regardless if these pubcrawls arent the greatest clubbing experience, it is possible to nevertheless satisfy really buddys in this way. The free ...
The Terminix Crawl Space Temp-Vent is a foundation ventilator that helps protect your home from the damage moisture and humidity can cause.
Archived from groups: rec.games.roguelike.misc (http://www.tomshardware.com/site/forums-usenet-faq.html)HihoFollowing Suggestion for Future Versions of Crawl:One should be able to create something lik...
Front crawl (FC) and back crawl (BC) are similar in terms of having alternating contributions of the arms combined with a six beat kick. However, the reason for the faster swimming times of FC than BC has not yet been ...
Im still alive! - Sorry to have been so absent for so long. Its been a lot this beginning of the year. The good news is everyone I know and love is still alive so far - a p... ...
The breast crawl is a super cool experience - a great way to bond, initiate breastfeeding, and its empowering to see what your body and your baby can do.
I woke up yesterday and had a not so nice encounter involving my head being perched on the side of the toilet bowl resulting in me making it in to work to come home again and crawl into bed. The tummy bug gremlins have infiltrated my system. And it seems the only thing I can think…
The in vitro anthelmintic evaluation of the crude extracts and solvent partitions on Pheretima posthuma and Ascarissuum (eggs and L2 stage) respectively showed significant results at the same concentration (10, 20, 40 and 80 mg/ml). This study provides a clear evidence for usage of Mucuna pruriens and Canarium schweinfurthii as an anthelmintic. The most potent ovicidal partition of C. schweinfurthii (Engl) leaves and bark and leaves of M. pruriens (DC) is hexane (100.00 ± 0.33), chloroform (98.20 ± 0.12) and chloroform (98.70 ± 0.88) partitioned crude respectively at 80 mg/ml while the most potent larvicidal partition is hexane (0.06%), chloroform (0.1%) and chloroform (0.2%). The active compounds are predominantly in the non-polar solvent which supports the tegumental diffusing activity of the compounds.
The examination was performed on 81 fecal samples, collected from swine, which came from three farms located in Dolnoslaskie and Wielkopolskie provinces, and on 42 samples from dogs from experimental breeding and dogs belongs to private owners. With both methods, as well F lleborns method as Fecalyzer method, infection in swine of coccidia (Coccidia) and nematods: Oesophagostomum spp. and Ascaris suum was detected. While, in dog feces the eggs of large roundworm Toxascaris leonina and whipworm Trichuris vulpis was detected. Method with Fecalyzer turned out slightly more sensitive than F lleborns method in case of infection of A. suum in swine and T. vulpis in dogs, and differences in prevalence of infection with this parasites amounted suitably 1.2% and 2.4%. Besides, method with Fecalyzer was characterized by smaller labour intensity and was less time-consuming than F lleborns method. Optimal, recommended time after which should be made result reading in this method, should be at least 10 ...
The polyprotein allergens/antigens of nematodes (NPAs) are the only lipid binding proteins known to be produced as polyproteins. Cleavage of the large polyprotein precursors at regularly spaced proteinase cleavage sites produces 10 or 11 individual protein units of similar to 15 kDa. The sequences of these units are highly diverse within and between species, but there are five absolutely or strongly conserved amino acid positions (Trp 15, Gln20, Leu42, Cys64, and Cys120). We have tested the role of these signature amino acids by mutational or chemical alteration of the ABA-1 protein of Ascaris, and examined the resulting modified proteins for perturbations of their lipid binding activities and structural integrity. Substitution of Trp15 and Gln20 both affect the stability of the protein in terms of resistance to thermal or chemical denaturation, but the ligand binding function is unaffected. Mutation of Leu42, however, disrupts both the proteins structural stability and functional integrity, as ...
Roundworms (nematodes) are common in poultry, waterfowl, and wild birds. Species of roundworms that affect poultry include species of large roundworms (Ascaris sp., also known as ascarids), species of small roundworms (Capillaria sp., also known as capillary worms or threadworms), and cecal worms (Heterakis gallinarum). Roundworms can cause significant damage to the organ(s) they infest. Most roundworms affect the digestive tract; others affect the trachea (windpipe) or eyes.. Large roundworms are the most damaging of the worms common to backyard flocks. A severe infestation can cause a reduction in nutrient absorption, intestinal blockage, and death. Easily seen with the naked eye, large roundworms are about the thickness of a pencil lead and grow to 4-1/2 inches long. Occasionally, they migrate up a hens reproductive tract and become included in a developing egg. The life cycle of a roundworm is direct; that is, worm eggs are passed in the droppings of infected birds and then directly to ...
For poultry, pigeons and pigs. For the treatment of Round Worms (Ascaridia Galli) in Poultry (Ascaridia columbae) in Pigeons; Round Worms (Ascaris suum)...
Edouard Joseph Marie Van Beneden (Leuven, 5 March 1846 - Liège, 28 April 1910), cytologist and marine biologist. He was professor of zoology at the University of Liège.[1] He contributed to cytology and genetics by his work on the roundworm Ascaris. In this work he discovered how chromosomes combined during meiosis, during the production of gametes.[2]. Beneden discovered, with Walther Flemming and Eduard Strasburger, the essential facts of mitosis. In contrast to meiosis, the double (diploid) set of chromosomes in daughter cells is identical to those in the parent cells. Excepting rare accidents, there is no change in the genetic apparatus. In meiosis, only one set of chromosomes ends up in each gamete, a condition called haploid. ...
352 THE WILL IN NATURE.. et deinceps corpus movendo foret? At extemplo contra hanc magicam motricem obicies istam esse intra concretum sibi suumque hospitium naturale, idcirco hanc, etsi magicam vocitemus, tantum erit nominis detorsio et abusus, siquidem vera et superstitiosa magica non ex anima basin desumit; cum eadem haec nil quidquam valeat, extra corpus suum movere altera re aut ciere. Respondeo vim et magicam illam naturalem animae, quae extra se agat virtute imaginis Dei latere iam obscuram in homine velut obdormire (post praevaricationem) excitationisque indigam: quae eadem, utut somnolenta, ac velut ebria, alioqui sit in nobis cottidie: sufficit tamen ad obeunda munia in corpore suo: dormit itaque scientia et potestas magica et solo nutu actrix in homine. [The soul, which is absolutely and entirely spirit, would never be able to move or excite the breath of life (which is thus physical and material) and still less flesh and bones, unless a certain power, naturally inherent in it, yet ...
Atlas Master Companies offers professional crawl space repair, encapsulation, vapor barriers, and more crawl space drainage solutions in Virginia. Call us today for a free quote!
Dungeon Crawl Stone Soup version 0.10-a0-1015-gffe86cf character file. 1518518 clouded the Bringer of Life (level 23, 204/218 HPs) Began as a Deep Dwarf Assassin on Sept 29, 2011. Was the Champion of Elyvilon. Escaped with the Orb ... and 3 runes on Sept 30, 2011! The game lasted 06:59:47 (66032 turns). clouded the Bringer of Life (Deep Dwarf Assassin) Turns: 66032, Time: 06:59:47 HP 204/218 AC 36 Str 27 XL: 23 Next: 59% MP 38/42 EV 13 Int 18 God: Elyvilon [******] Gold 5143 SH 43 Dex 19 Spells: 2 memorised, 27 levels left Res.Fire : + + . See Invis. : . a - +8,+9 broad axe (freeze) Res.Cold : x . . Warding : . . R - +8 dragon armour Life Prot.: + . . Conserve : + U - -1 large shield Res.Acid. : . . . Res.Corr. : . S - +2 helmet of Obohoheh {Str+5 Dex+1} Res.Poison: + Clarity : . J - +1 cloak Strowk {+Blink} Res.Elec. : + Spirit.Shd : . l - +1 pair of dwarf gloves Sust.Abil.: . . Stasis : . L - +2 pair of boots of the Monkeys Uncle {rE Res.Mut. : . Ctrl.Telep.: x v - amulet of conservation ...
Clarke Basement Systems is Blackstocks local expert for Basement Waterproofing, Foundation & Crawl Space Encapsulation. Schedule a FREE estimate today!
recurrence=NONE, maxDuration=86400, maxDocumentCount=null, isTestCrawl=false, isPatchCrawl=false, oneTimeSubtype=MISSING_URLS_PATCH_CRAWL, seedCount=15,...
Clarke Basement Systems is a locally proven basement waterproofing, foundation & crawl space encapsulation contractor serving Caesarea, ON. Schedule a free estimate!
jobId=167867, recurrence=NONE, maxDuration=600, maxDocumentCount=null, isTestCrawl=false, isPatchCrawl=false, oneTimeSubtype=CRAWL_SELECTED_SEEDS, seedCount=1,...
If youve had an animal or pest living in your crawl space, call Attic Kings for a free inspection. Well clear out any debris and decontaminate the area.
Parasitic nematodes, also know as roundworms, cause diseases in a variety of organs. Their larva can cause disease by migrating through the body.
I have a one year old house on a crawl space that is lined with plastic. The indoor humidity is 60-70%. Have a central heat and air unit outside, gas heat, electric air, ducted through crawl space. I live in TN where it is humid. A Ultra air 90 whole house dehumidifier has been recommended. My concerns are that this will be placed in the crawl space and I am concerned about mold growth on the coils of the unit. Has this been a problem with anyone else? Also, the diagrams for installation
The WebCrawler worker is started with one input bulk that contains records with URLS to crawl. (The exception to this rule is the start of the crawl process where it gets a task without an input bulk, which causes it to generate an input record from the task parameters or configuration in a later version.). Then the components are executed like this: ...
Many homeowners searching the internet for crawlspace repair information end up in home repair forums. While there is some good information out there, there are many examples of bad and outdated advice...
The iron will of Roger Federer and the burning desire of Novak Djokovic carried them into the quarter-finals of the US Open on Tuesday.
Newcomers to Recovery - ........ - Really struggling tonight, had a real barny with my wife and stormed out the house for a while ive given myself every excuse to drink tonight. Were sitting in angry silence right now and im craving to the point its making my skin crawl. It doesnt help that she keeps shouting at me
Basements, Attics and Crawl Spaces - Attic/crawlspace access of a cross-gable type roof - Hello, all - Im a long-time lurker and consumer of advice and browsing on the forum, and first-tim
Feed Type Link http://www.venturedeal.com/Search/SearchResultTransactionDetail.aspx?TransactionId=f2fbc072-7aba-408a-85a3-736639dae220 Date 12/1/2016
Ascaris suum, pig-infecting giant roundworm, closely related to human-infecting giant roundworm Ascaris lumbricoides (2011) ... October 2011). "Ascaris suum draft genome". Nature. 479 (7374): 529-33. Bibcode:2011Natur.479..529J. doi:10.1038/nature10553. ...
Ascaris suum) Mollusca • Owl limpet (Lottia gigantea) • Pacific oyster (Crassostrea gigas) Annelida • Polychaete worm ( ...
In pigs, the infection is caused by Ascaris suum. It is characterized by poor weight gain, leading to financial losses for the ... Ascaris lumbricoides adult worms Ascaris egg, incubation process: The Ascaris egg incubation process consists in placing the ... Prophylactic activity in a mouse-ascaris suum test model". The Journal of Parasitology. 57 (3): 487-93. doi:10.2307/3277899. ... The larva of Ascaris lumbricoides developing in the egg Ascaris lumbricoides adult worms (with measuring tape for scale) ...
Müller S, Walter RD (April 1992). "Purification and characterization of polyamine oxidase from Ascaris suum". The Biochemical ...
"Crystal structure of mitochondrial quinol-fumarate reductase from the parasitic nematode Ascaris suum". Journal of Biochemistry ...
Morphine increases the latency of this defensive response in the parasitic Ascaris suum. In a study on the effects of opiates ... "The effect of opiates and opiate antagonists on heat latency response in the parasitic nematode Ascaris suum". Life Sciences. ...
Ascaris suum) infections; aid in the prevention of establishment of nodular worm (Oesophagostomum) infections. In animal models ...
In Ascaris suum, two MSP-fiber proteins (MFP), MFP1 and 2, with opposite effect on polymerization have been identified. MFP1 ... Molecular structures of MSP from Ascaris suum and Caenorhabditis elegans have been determined by X-ray crystallography and NMR ... of Ascaris suum". J. Mol. Biol. 263 (2): 284-96. doi:10.1006/jmbi.1996.0575. PMID 8913307. Haaf A, LeClaire L, Roberts G, Kent ... of Ascaris suum - evidence for two manganese binding sites and the possible role of divalent cations in filament formation". J ...
Ligocka, A.; Paluszak, Z. (2009). "Effectiveness of different sanitisation technologies on the inactivation of Ascaris suum ... There is also evidence that mesophilic (ambient temperature) fermentation kills eggs of the Ascaris worm - a parasite of humans ...
Nematodes causing such zoonotic infections are Baylisascaris procyonis, Toxocara canis, Toxocara cati, and Ascaris suum. These ... "Pulmonary Lesions Associated with Visceral Larva Migrans Due to Ascaris suum or Toxocara canis: Imaging of Six Cases". American ...
Roepstorff A, Murrell KD (May 1997). "Transmission dynamics of helminth parasites of pigs on continuous pasture: Ascaris suum ...
In its native habitat, this species preys on eggs of the parasite Ascaris suum, which survive and develop after passage through ... The role of mollusks as benthos components in purification of water bodies from Ascaris suum eggs. Paraziologiya 9(5):432-433. ...
"The effect of opiates and opiate antagonists on heat latency response in the parasitic nematode Ascaris suum". Life Sciences. ...
In some eukaryotes, such as the parasitic worm Ascaris suum, an enzyme similar to complex II, fumarate reductase (menaquinol: ... "Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum". Biochim ...
"Crystal Structure of the Malic Enzyme from Ascaris suum Complexed with Nicotinamide Adenine Dinucleotide at 2.3 Å Resolution". ...
Ascaris suum). PI-3 occupies the active site of pepsin using its N-terminal residues and thereby blocks substrate binding. ... August 2000). "Structural basis for the inhibition of porcine pepsin by Ascaris pepsin inhibitor-3". Nature Structural Biology ...
... identification of extracts with potent activity against Ascaris suum in vitro". Parasite. 23: 24. doi:10.1051/parasite/2016024 ... showed than ethanolic extracts of Clausena anisata have in vitro anthelmintic properties against the nematode Ascaris suum, a ...
An antibacterial peptide from Ascaris suum, a parasitic nematode that resides in the pig intestine, also belongs to this family ...
... zanthoxyloides have in vitro anthelmintic properties against the nematode Ascaris suum, a swine parasite that is closely ... identification of extracts with potent activity against Ascaris suum in vitro". Parasite. 23: 24. doi:10.1051/parasite/2016024 ...
Komuniecki R, McCrury J, Thissen J, Rubin N (1989). "Electron-transfer flavoprotein from anaerobic Ascaris suum mitochondria ...
... found in some copepods and roundworms such as Ascaris suum, portions of the chromosomes are cast away in particular cells. This ... In A. suum, all the somatic cell precursors undergo chromatin diminution. X-inactivation. The inactivation of one X chromosome ...
... and initiation factors to nematode translation in an Ascaris suum embryo cell-free system". J. Biol. Chem. 279 (44): 45573-85. ...
... of the genus Ascaris (giant intestinal roundworms). The large roundworm of pigs (Ascaris suum) typically infects pigs while ... Piperazine, a flaccid paralyzing agent that causes a blocking response of ascaris muscle to acetylcholine. The narcotizing ... Ascaris lumbricoides affects human populations, typically in sub-tropical and tropical areas with poor sanitation. Ascaricides ...
... these isoforms are also found in Ascaris suum and Caenorhabditis elegans. The subunits form a membrane-bound cytochrome b ...
Ascaris suum". J Biol Chem. 260 (8): 4770-4777. PMID 3988734. Kionka, C.; Kunau, W.H. (1985). "Inducible β-oxidation pathway in ...
Pada beberapa eukariota seperti cacing parasit Ascaris suum, terdapat enzim yang mirip dengan kompleks II, yaitu fumarat ... "Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum". Biochim ...
Ancylostoma caninum Ancylostoma duodenale Ascaris suum Dicrocoelium dendriticum Dictyocaulus viviparus Dipylidium caninum ...
... ascaris MeSH B01.500.500.294.700.100.100.108.425 - ascaris lumbricoides MeSH B01.500.500.294.700.100.100.108.700 - ascaris suum ...
... distant homologs.Homologous sequences were found in species as distantly related to humans as the pig roundworm Ascaris suum, ...
... including the nematodes such as Ascaris suum, Ascaris lumbricoides, Ascaridia galli, Heterakis gallinarum, a cestode ...
U niektórych eukariotów, takich jak należąca do pasożytniczych nicieni Ascaris suum, enzym zbliżony do kompleksu II, reduktaza ... Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum. „Biochim ...
In some eukaryotes, such as the parasitic worm Ascaris suum, an enzyme similar to complex II, fumarate reductase (menaquinol: ... "Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum". Biochim ...
기생충인 돼지회충(Ascaris suum)과 같은 일부 진핵생물에서 복합체 II 와 유사한 효소인 푸마르산 환원효소(메나퀴놀:푸마르산 산화환원효소)는 유비퀴놀을 산화시키고 푸마르산을 환원시키는 역반응을 촉매한다. 이것은 ... "Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum". 《Biochim ...
... found in some copepods and roundworms such as Ascaris suum, portions of the chromosomes are cast away in particular cells. This ... suum, all the somatic cell precursors undergo chromatin diminution.[28] ...
പരാദവിരയായ നാടവിര (Ascaris suum) പോലുള്ള ചില യൂക്കാരിയോട്ടുകളിൽ, സംയുക്തം II വിന് സമാനമായ ഒരു എൻസൈം, ഫ്യൂമറേറ്റ് റിഡക്ടേസ് ( ... "Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum". Biochim ...
Roepstorff A, Murrell KD (May 1997). "Transmission dynamics of helminth parasites of pigs on continuous pasture: Ascaris suum ...
The first crucial step in understanding the life cycle of the roundworm Ascaris lumbricoides was demonstrated by Grassi in a ... CENTENARIO DELLA NASCITA MENTRE NEL MONDO SI AVVERA IL SUO SOGNO D'UMANA REDENZIONE DAL SECOLARE FLAGELLO MALARICO 27 MARZO ... He was the first to demonstrate the direct life cycle of the roundworm Ascaris lumbricoides by self-experimentation. He ...
The Sentinel Chamber with Ascaris Suum Eggs. Sentinel Chambers are loaded with 100,000 Ascaris eggs and wastewater or biosolids ... Ascaris suum eggs from pig feces. Eggs are highly viable and supplied with documentation certifying the percentage of eggs that ... The Ascaris suum eggs used have received no treatment with harmful chemicals and are recovered from the feces of naturally ... Product List , Test Organisms Available , Ascaris suum eggs , Cryptosporidium Parvum oocysts , Ordering Information. ...
"Are Ascaris lumbricoides and Ascaris suum a single species?". Parasites & Vectors. 5: 42. doi:10.1186/1756-3305-5-42. ISSN 1756 ... Ascaris eggs can remain infective for years in the soil, even in a temperate climate. The migration of A. suum larvae through ... A. suum is distributed worldwide and grows up to 40 cm (16 in) in length. Ascaris infections are treated with ascaricides. A. ... Ascaris suum, also known as the large roundworm of pig, is a parasitic nematode that causes ascariasis in pigs. While ...
... Author(s). Leeuw, W.A. de; Borgsteede, F.H.M.; Over, H.J.; Burg, W.P.J. van ...
"Crystallographic studies on Ascaris suum NAD-malic enzyme bound to reduced cofactor and identification of an effector site.". ... "Crystallographic studies on Ascaris suum NAD-malic enzyme bound to reduced cofactor and identification of an effector site.". ... "Crystallographic studies on Ascaris suum NAD-malic enzyme bound to reduced cofactor and identification of an effector site.". ... "Crystallographic studies on Ascaris suum NAD-malic enzyme bound to reduced cofactor and identification of an effector site.". ...
Ascaris suum (Pig roundworm) (Ascaris lumbricoides). Ascaris lumbricoides (Giant roundworm). 1,051. UniRef100_U1M6F5. Cluster: ... Ascaris suum (Pig roundworm) (Ascaris lumbricoides). Ascaris lumbricoides (Giant roundworm). 1,051. UniRef90_U1M6F5. Cluster: ... Ascaris suum (Pig roundworm) (Ascaris lumbricoides). Ascaris lumbricoides (Giant roundworm). 1,051. UniRef50_U1M6F5. Cluster: ... Ascaris suum (Pig roundworm) (Ascaris lumbricoides)Imported. ,p>Information which has been imported from another database using ...
... and initial velocity studies suggest a rapid-equilibrium random sequential mechanism for the Ascaris suum malic enzyme. The ... Antisera prepared against A. suum malic enzyme reacted to a small extent with the NAD malic enzymes from two free-living ... A correlation coefficient of 0.911 was obtained upon comparing the amino acid composition of A. suum and E. coli malic enzymes ... A procedure for the isolation of malic enzyme from muscle tissue of the roundworm Ascaris suum is described. The fractionation ...
The levels of trehalose and the activity of trehalase during the development of Ascaris suum eggs were investigated. The level ... 13] Murrel, K. D., Eriksen, L., Nansen, P., Slotved, H.-C., Rasmussen, T. (1997): Ascaris suum: a revision of its early ... 7] Dubinský, P., Ryboš, M., Turčeková, L. (1980): Distribution of glycogen in Ascaris suum and Parascaris equorum (Nematoda) ... 6] Dmitryjuk, M., Żółtowska, K. (2004): Trehalose catabolism enzymes in tissues of Ascaris suum (Nematoda). Helminthologia, 41 ...
The malic enzyme gene of Ascaris suum was cloned into the vector pTRC99a in two forms encoding alternative amino-termini. The ... Metabolic engineering succinic acid Escherichia coli malic enzyme Ascaris suum Author to whom all correspondence and reprint ... Expression of Ascaris suum Malic Enzyme in a Mutant Escherichia coli Allows Production of Succinic Acid from Glucose. ... Stols L., Kulkarni G., Harris B.G., Donnelly M.I. (1997) Expression of Ascaris suum Malic Enzyme in a Mutant Escherichia coli ...
Crystal structure of the malic enzyme from Ascaris suum complexed with nicotinamide adenine dinucleotide at 2.3 A resolution. ... Pig roundworm (Ascaris suum) [TaxId: 6253] B296-593. d1llqb1. Alpha and beta proteins (a/b) NAD(P)-binding Rossmann-fold ... Pig roundworm (Ascaris suum) [TaxId: 6253] A2-295. d1llqa2. Alpha and beta proteins (a/b) Aminoacid dehydrogenase-like, N- ... Pig roundworm (Ascaris suum) [TaxId: 6253] B2-295. d1llqb2. Alpha and beta proteins (a/b) Aminoacid dehydrogenase-like, N- ...
... japonicum and A.suum. This is the first report on the codon usage biology in S. japonicum and A.suum, and the factors ... AbstractSchistosoma japonicum and Ascaris suum are considered as the major parasites of human which cause various life ... Comparative analyses of the complete mitochondrial genomes of Ascaris lumbricoides and Ascaris suum from humans and pigs. Gene ... Keywords: Schistosoma japonicum; Ascaris suum; codon usage bias; compositional properties; expression level; neutrality plot ...
We have developed a method for dissecting single neurons from the nematode Ascaris suum, in order to determine their peptide ... We have developed a method for dissecting single neurons from the nematode Ascaris suum, in order to determine their peptide ... expression by mass spectrometry in single dissected identified neurons from the dorsal ganglion of the nematode Ascaris suum ...
In general, Ascaris larval antigen and AP elicited larger intradermal reactions than other antigens in Ascaris-infected animals ... In addition, Ascaris perienteric fluid (AP) provoked skin responses in these animals of a magnitude similar to those induced by ... The reciprocal relationship, i.e., comparable reactivity of AP and TL in Ascaris-infected animals, was not apparent. ... The results of this study indicated no superiority of adult antigens in differentiating Ascaris and Toxocara infections. ...
... and the closely related Ascaris suum in pigs. Progress has been made in understanding interactions between helminths and host ... and the closely related Ascaris suum in pigs. Progress has been made in understanding interactions between helminths and host ... ESP from different A. suum life stages (in vitro-hatched L3, lung-stage L3, L4, and adult) as well as BF from adult males were ... ESP from different A. suum life stages (in vitro-hatched L3, lung-stage L3, L4, and adult) as well as BF from adult males were ...
These results suggest that A. suum contains both an allergen and a mitogenic substance. ... Ascaris suum extract was found to contain a mitogenic factor which stimulates human lymphocytes. The extract (100 micrograms ... Ascaris suum: human lymphocyte mitogenic factor content.. @article{Sasagawa1987AscarisSH, title={Ascaris suum: human lymphocyte ... Ascaris suum extract was found to contain a mitogenic factor which stimulates human lymphocytes. The extract (100 micrograms ...
Our findings suggest a wider infection range with a possible transmission of A. suum to domestic animals that interact with ... An aberrant Ascaris suum infection in a domestic dog in China in 2019 is described for the first time. This pathogen is a ... Ascaris suum is a common roundworm of pigs. Although pig husbandry has become more modernized and industrialized, A. suum is ... First report on aberrant Ascaris suum infection in a dog, China. *Yue Xie. ORCID: orcid.org/0000-0001-9062-69281. na1, ...
High molecular weight components containingN-linked oligosaccharides ofAscaris suumextract inhibit the dendritic cells ... High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). The ...
"Protective immunity elicited by the nematode-conserved As37 recombinant protein against Ascaris suum infection, PLoS Neglected ... 2004) Recombinant Ascaris 16-Kilodalton protein-induced protection against Ascaris suum larval migration after intranasal ... 2004) Recombinant Ascaris 16-Kilodalton protein-induced protection against Ascaris suum larval migration after intranasal ... 17 Protective immunity elicited by As37 recombinant protein against Ascaris suum infection from Ascaris related complications ...
The in vitro anthelmintic evaluation of the crude extracts and solvent partitions on Pheretima posthuma and Ascarissuum (eggs ... Effect of the extracts on the Unembryonated Eggs of Ascaris suum. The effect of the leaves and bark of C. schweinfurthii (Engl ... on Ascaris suum. Open Access Library Journal,02,1-8. doi: 10.4236/oalib.1101102 ... The unembryonated egg of A. suum is the non- infectious stage of the parasite; the objective is to prevent the unembryonated ...
L. Tilleman et al., "A globin domain in a neuronal transmembrane receptor of Caenorhabditis elegans and Ascaris suum: molecular ... 2015). A globin domain in a neuronal transmembrane receptor of Caenorhabditis elegans and Ascaris suum: molecular modeling and ... A globin domain in a neuronal transmembrane receptor of Caenorhabditis elegans and Ascaris suum: molecular modeling and ... Comprehensive sequence similarity searches in the genome of the parasitic nematode, Ascaris suum, revealed a chimeric protein ...
Here the IVRS from L2 and L3/4 stages of Ascaris lumbricoides and Ascaris suum were examined for the first time both ... Immunochemical and Biochemical Analysis of Larval Secreted Antigens from the Parasitic Nematodes Ascaris suum and Ascaris ... Ascaris lumbricoides and Ascaris suum respectively. The importance of detailed biological examination of the IVRS from these ... suum IVRS showed that, except for one product from A. suum L3/4 IVRS, all the secretions of A. suum L2 and L3/4 worms are ...
Versus Conventional Composting of Human Feces with Ascaris suum Ova as Model Helminthic Parasites ... The Effectiveness and Safety of Vermi- Versus Conventional Composting of Human Feces with Ascaris suum Ova as Model Helminthic ... in an experiment with highly concentrated and viable Ascaris suum (2626±1306 ova/g, 61.6±8.7% viable) inoculated into fecal ... vermicomposting was found ineffective at reducing Ascaris suum ova concentration and viability. Decentralized vermicomposting ...
Human macrophages were able to adhere to and trap A suum larvae in the presence of either human or pig serum containing Ascaris ... Human macrophages were able to adhere to and trap A suum larvae in the presence of either human or pig serum containing Ascaris ... Human macrophages were able to adhere to and trap A suum larvae in the presence of either human or pig serum containing Ascaris ... Human macrophages were able to adhere to and trap A suum larvae in the presence of either human or pig serum containing Ascaris ...
Ascariasis is an important infection in humans (Ascaris lumbricoides) and pigs (Ascaris suum) and individuals appear to be ... Lewis, R., Behnke, J.M., Cassidy, J., Stafford, P., Murray, N. and Holland, C.V., The migration of Ascaris suum larvae, and the ... The migration of Ascaris suum larvae, and the associated pulmonary inflammatory response in susceptible C57BL/6j and resistant ... The migration of Ascaris suum larvae, and the associated pulmonary inflammatory response in susceptible C57BL 6j and resistant ...
I De Baere, L Liu, L Moens, J Van Beeumen, C Gielens, J Richelle, C Trotman, J Finch, M Gerstein, M Perutz (1992). Proc Natl Acad Sci U S A 89: 4638-42 ...
The mean intensity of infection with migrating Ascaris larvae in HgC1(2)-treated animals increased by 15%, compared with ... The subchronic effect of mercury on selected immunological parameters was studied in guinea pigs with experimental Ascaris suum ... Effect of heavy metal intoxication on macrophage metabolic activity of mice infected with Ascaris suum. M. Jalčová, Emília ... Effect of mercury on the immune response and mean intensity of Ascaris suum infection in guinea pigs.. @article{ ...
About Ascaris suum The nematode Ascaris suum is the large roundworm parasite of pigs and the causative agent of ascariasis. It ... Ascaris suum BioProject PRJNA62057 , Data Source University of Colorado School of Medicine , Taxonomy ID 6253 ... There is 1 alternative genome project for Ascaris suum available in WormBase ParaSite: PRJNA80881 ... The A. suum genome has been sequenced by the Davis laboratory at the University of Colorado in collaboration with the Mitreva ...
The nematode Ascaris suum is the large roundworm parasite of pigs and the causative agent of ascariasis. It is closely related ... There are 2 genome projects for Ascaris suum:*PRJNA62057: University of Colorado Denver ... Cross infections of A. suum to humans and A. lumbricoides to pigs has been reported. ...
How is Ascaris suum spread to humans?. The adult Ascaris suum worm lives in the pigs intestines and produces eggs that are ... Ascaris suum is not transmitted to people by eating pork or pork products because the worm eggs are not found in the meat. ... Who is at risk for infection with Ascaris suum?. People who raise pigs or use raw pig manure as fertilizer may be at risk for ... How do I know if my pigs are infected with Ascaris suum?. Many infected pigs do not show any symptoms. Heavily infected pigs ...
WormBase ParaSite is an open access resource providing genome sequences, genome browsers, semi-automatic annotation and comparative genomics analysis for nematode and platyhelminth parasites (helminths).
  • Pigs get infected with A. suum by ingesting infectious parasite eggs that are present in the environment. (wikipedia.org)
  • Ascaris eggs can remain infective for years in the soil, even in a temperate climate. (wikipedia.org)
  • Sentinel Chambers are loaded with 100,000 Ascaris eggs and wastewater or biosolids taken directly from the treatment plant to be tested. (lightlink.com)
  • Our basic Sentinel Chamber containing Ascaris eggs, has two stainless steel mesh screens with 30 micron pores. (lightlink.com)
  • The Ascaris suum eggs used have received no treatment with harmful chemicals and are recovered from the feces of naturally infected pigs. (lightlink.com)
  • The levels of trehalose and the activity of trehalase during the development of Ascaris suum eggs were investigated. (degruyter.com)
  • 3] Beis, L., Barrett J. (1975): Energy metabolism in developing Ascaris lumbricoides eggs. (degruyter.com)
  • 8] Fairbairn, D., Passey, R. F. (1957): Occurrence and distribution of trehalose and glycogen in the eggs and tissues of Ascaris lumbricoides. (degruyter.com)
  • The in vitro anthelmintic evaluation of the crude extracts and solvent partitions on Pheretima posthuma and Ascarissuum (eggs and L2 stage) respectively showed significant results at the same concentration (10, 20, 40 and 80 mg/ml). (scirp.org)
  • Thus mice were inoculated with fully embryonated A. suum eggs and larval burdens were enumerated in the large intestine and rectum, liver and lungs of the 2 strains at 6 h post-inoculation (p.i.) and on each of days 1-8 p.i. inclusively. (tcd.ie)
  • The adult Ascaris suum worm lives in the pig's intestines and produces eggs that are passed in the pigs' feces (manure). (cdc.gov)
  • Ascaris suum infection is caused by ingesting these infective eggs. (cdc.gov)
  • Ascaris suum is not transmitted to people by eating pork or pork products because the worm eggs are not found in the meat. (cdc.gov)
  • The objective of this study was to evaluate the effects of several different commercial disinfectants on the embryogenic development of Ascaris suum eggs. (parasitol.kr)
  • A 1-ml aliquot of each disinfectant was mixed with approximately 40,000 decorticated or intact A. suum eggs in sterile tubes. (parasitol.kr)
  • At 3 weeks of incubation after exposure, ethanol, methanol, and chlorohexidin treatments did not affect the larval development of A. suum eggs, regardless of their concentration and treatment time. (parasitol.kr)
  • When the albumin layer of A. suum eggs remained intact, however, even the 10% povidone iodine solution took at least 5 min to reasonably inactivate most eggs, but never completely kill them with even 60 min of exposure. (parasitol.kr)
  • This study demonstrated that the treatment of A. suum eggs with many commercially available disinfectants does not affect the embryonation. (parasitol.kr)
  • Although some disinfectants may delay or stop the embryonation of A. suum eggs, they can hardly kill them completely. (parasitol.kr)
  • Eggs of the genus Ascaris have the highest resistance and survive under numerous treatment conditions such as a variety of strong acids, strong bases, oxidants, reductants, protein-disrupting agents, and surface-active agents [ 9 ]. (parasitol.kr)
  • Due to its resistance to biocontrol mechanisms, A. suum eggs have been chosen as model eggs to measure treatment efficiency [ 10 ]. (parasitol.kr)
  • Furthermore, in previous reports, some disinfectants such as povidone-iodine showed discrepancy in efficacy against Ascaris eggs [ 14 - 16 ]. (parasitol.kr)
  • Peak 11 from elution of two preparations of metabolic antigens protected mice against a chállenge infection of 10,000 A. suum embryonated eggs. (unifesp.br)
  • The influence of temperature on the development and embryonation of Ascaris suum eggs was studied using coarse sand medium in an environmental chamber with 50% humidity. (parasitol.kr)
  • The formation of larvae in A. suum eggs at temperatures of 35℃ and 25℃ appeared at days 17 and 19 after incubation, respectively. (parasitol.kr)
  • These findings show that 35℃ condition shortens the time for the development of A. suum eggs to the 8-cell-stage in comparison to 25℃, and suggest the possibility of accelerated transmission of this parasite, resulting from global warming and ecosystem changes. (parasitol.kr)
  • Ascaris lumbricoides , a soil-transmitted nematode, can cause human infections through ingestion of food contaminated with embryonated eggs [ 1 ]. (parasitol.kr)
  • In addition, an in vitro test for embryonation of eggs showed that A. suum eggs could be used as a model for A. lumbricoides [ 3 , 4 ]. (parasitol.kr)
  • The effect of different reagents on the viability of Ascaris suum eggs during laboratory detection and quantification was assessed and different incubation solutions compared. (environmental-expert.com)
  • Parasitologic examination of the hay bale showed contamination with A. suum nematode eggs, indicating that the hay bale was probably exposed to pig feces. (cdc.gov)
  • The viability of Ascaris suum eggs, a conservative indicator of fecal pollution, and its association with ammonia was investigated in separated liquid slurry in comparison with raw slurry. (forskningsdatabasen.dk)
  • For this purpose nylon bags with 6000 eggs each were placed in 1 litre bottles containing one of the two fractions for 308 days at 5 °C or 25 °C. Initial analysis of helminth eggs in the separated liquid slurry revealed 47 Ascaris eggs per gramme. (forskningsdatabasen.dk)
  • In this study, researchers from the U.S. Military Academy, Cornell University, and ARS J. Phil Campbell, Sr., Natural Resource Conservation Center evaluated the efficacy of short chained fatty acids (FA) on inactivating Ascaris eggs in water and sewage sludge. (usda.gov)
  • From this study a model was developed that predicts the toxicity of FA based concentration and level of acidity (pH) on Ascaris eggs. (usda.gov)
  • Ascaris suum eggs were inactivated in distilled water and digested sludge by butanoic, pentanoic and hexanoic acids. (usda.gov)
  • After ingestion of Ascaris lumbricoides eggs, larvae hatch in the intestine and penetrate the mesenteric lymphatics and venules to enter the pulmonary circulation. (medscape.com)
  • Infection occurs through ingestion of food contaminated with fecal matter containing Ascaris eggs . (bionity.com)
  • A 1978 study showed about 75% of all sewage sludge samples sampled in United States urban catchments contained Ascaris ova, with rates as high as 5 to 100 eggs per litre [ citation needed ] . (bionity.com)
  • As37 is an immunodominant antigen Published: February 13, 2020 of Ascaris suum, a pig roundworm closely related to the human A. lumbricoides parasite, recognized by protective immune sera from A. suum infected mice. (deepdyve.com)
  • Additionally there was also extensive crossreactivity of antiserum from rabbits infected with the ascaridoidean parasite Toxocara canis and the secreted antigens of both A. suum and A. lumbricoides. (gla.ac.uk)
  • In the current study, we investigated the capacity of human macrophages to recognize and attack larval stages of Ascaris suum, a natural porcine parasite that is genetically similar to the human helminth Ascaris lumbricoides. (monash.edu)
  • The nematode Ascaris suum is the large roundworm parasite of pigs and the causative agent of ascariasis. (wormbase.org)
  • Ascaris suum is an intestinal parasite of pigs that can also infect people. (cdc.gov)
  • in addition to its veterinary significance it is a good model of the human parasite Ascaris lumbricoides, estimated to infect approximately 1.4 billion people globally. (ox.ac.uk)
  • A. lumbricoides and A. suum are closely related ascarid species and both species constitute natural host-parasite relationships with similar life cycles. (parasitol.kr)
  • A. suum is an important zoonotic parasite, and genetic studies showed that A. suum may infect humans also [ 12 - 14 ]. (parasitol.kr)
  • The Ascaris suum nematode is a common parasite of swine. (cdc.gov)
  • Ascaris suum is the largest intestinal parasite of pigs with higher prevalence in free range and organic production. (wcgalp.org)
  • The closely related swine parasite Ascaris suum is used routinely as a surrogate for testing methods designed to disinfect A. lubricoides. (usda.gov)
  • Ascaris suum, also known as the large roundworm of pig, is a parasitic nematode that causes ascariasis in pigs. (wikipedia.org)
  • A procedure for the isolation of malic enzyme from muscle tissue of the roundworm Ascaris suum is described. (unt.edu)
  • Ascariasis is a widespread soil-transmitted helminth infection caused by the intestinal roundworm Ascaris lumbricoides in humans, and the closely related Ascaris suum in pigs. (frontiersin.org)
  • Ascaris suum is a common roundworm of pigs. (biomedcentral.com)
  • It is evident that A. suum is zoonotic and its cross-infection and hybridization with the human roundworm Ascaris lumbricoides has been recently confirmed in areas of human-pig sympatry [ 2 ]. (biomedcentral.com)
  • There is a relatively small amount of experimental data regarding the in vitro - released secretory products (IVRS) of the parasitic roundworm nematodes of man and swine, Ascaris lumbricoides and Ascaris suum respectively. (gla.ac.uk)
  • Antigenic Analysis of Isolated Tissues and Body Fluids of Roundworm, Ascaris Lumbricoides Va. Suum (3 Monographs by Oliver-Gonzalez, Jose: George Banta Publ. (abebooks.com)
  • In the present study, we conducted a large-scale investigation of TIMP proteins of a range of neglected human parasites including the hookworm Necator americanus , the roundworm Ascaris suum , the liver flukes Clonorchis sinensis and Opisthorchis viverrini , as well as the schistosome blood flukes. (biomedcentral.com)
  • Ascariasis is a human disease caused by the parasitic roundworm Ascaris lumbricoides . (bionity.com)
  • Comprehensive sequence similarity searches in the genome of the parasitic nematode, Ascaris suum, revealed a chimeric protein that is similar to a Phe-Met-Arg-Phe-amide neuropeptide receptor. (ugent.be)
  • The nicotinic acetylcholine receptors of the parasitic nematode Ascaris suum: formation of two distinct drug targets by varying the relative expression levels of two subunits. (ox.ac.uk)
  • In the present study, a cDNA pool was prepared from poly A + RNA isolated from pharynxes dissected from the parasitic nematode, Ascaris suum, and was used as a template for RT-PCR with degenerate primers designed from sequences conserved in 5-HT receptors from a variety of sources. (elsevier.com)
  • Here, we show that purified PAC strongly inhibit the activity of both recombinant and native GSTs from the parasitic nematode Ascaris suum . (legumeplus.eu)
  • The migration of A. suum larvae through the lungs may cause secondary bacterial infections and result in acute respiratory symptoms in pigs. (wikipedia.org)
  • The specificity and sensitivity of adult and larval somatic antigens, and perienteric fluid of Toxocara canis and Ascaris suum were investigated by using intradermal skin tests in guinea pigs. (ajtmh.org)
  • Ascariasis is an important infection in humans (Ascaris lumbricoides) and pigs (Ascaris suum) and individuals appear to be predisposed to either heavy or light worm burdens. (tcd.ie)
  • Effect of mercury on the immune response and mean intensity of Ascaris suum infection in guinea pigs. (semanticscholar.org)
  • The subchronic effect of mercury on selected immunological parameters was studied in guinea pigs with experimental Ascaris suum infection. (semanticscholar.org)
  • Cross infections of A. suum to humans and A. lumbricoides to pigs has been reported. (wormbase.org)
  • How do I know if my pigs are infected with Ascaris suum ? (cdc.gov)
  • People who raise pigs or use raw pig manure as fertilizer may be at risk for infection with Ascaris suum . (cdc.gov)
  • Consult a veterinarian for recommendations on preventing and controlling Ascaris suum in your pigs. (cdc.gov)
  • Among them, Ascaris lumbricoides and Ascaris suum are now considered to be a single species that can complete life cycle both in pigs and humans [ 2 ]. (parasitol.kr)
  • In order to determine mutations associated with benzimidazole resistance in Ascaris suum , worms were collected from slaughtered pigs and a partial region of the ß- tubulin gene was sequenced. (bvsalud.org)
  • Recent studies on soil-transmitted nematodes, especially the genus Ascaris , included those on A. suum in pigs as a model for A. lumbricoides infection of humans due to several reasons [ 2 ]. (parasitol.kr)
  • A. suum is a soil-transmitted nematode which can infect pigs and cattle, and it is known that the egg development and survival are affected by temperature [ 7 - 11 ]. (parasitol.kr)
  • In the Republic of Korea (=Korea), the infection rate of A. suum in pigs was reported to be about 17.6% by fecal examination in rural areas [ 12 ]. (parasitol.kr)
  • The nematode Ascaris suum remainsa major parasitic disease in national livestock pigs . (bvsalud.org)
  • The objective of this work was to evaluate the level of infestationby A. suum in pigs at slaughter age provenient from intensive production systems located in Zona da Mata region, latitude 20º45 45 S, longitude 43°18 10 W, in tropical Southeastern, Brazil . (bvsalud.org)
  • Effect of strategic deworming on Ascaris suum exposure and technical performance parameters in fattening pigs. (nih.gov)
  • Anti-Ascaris suum IgG antibodies in fattening pigs with different respiratory conditions. (nih.gov)
  • An aberrant Ascaris suum infection in a domestic dog in China in 2019 is described for the first time. (biomedcentral.com)
  • Our findings suggest a wider infection range with a possible transmission of A. suum to domestic animals that interact with humans. (biomedcentral.com)
  • Here we expand the currently recognized infection spectrum by firstly describing A. suum infection in domestic dogs in China. (biomedcentral.com)
  • Morphological characterization of Ascaris suum nematode infection in the lung of a dog in China, 2019. (biomedcentral.com)
  • https://doi.org/10.1371/journal.pntd.0008057 February 13, 2020 1 / 17 Protective immunity elicited by As37 recombinant protein against Ascaris suum infection conserved in other human STHs. (deepdyve.com)
  • Author summary Ascaris infection is the most common infection of humans living in poverty worldwide and can result in malnutrition and stunted physical and mental development in children. (deepdyve.com)
  • To develop a vaccine against Ascaris infection, an immunodominant antigen, As37 of A. suum, was cloned and expressed as a soluble recombinant protein in E. coli. (deepdyve.com)
  • Here the IVRS from L2 and L3/4 stages of Ascaris lumbricoides and Ascaris suum were examined for the first time both immunochemically and biochemically to evaluate their usefulness for specific detection of ascariasis and for possible use in vaccination of animals or humans against Ascaris infection. (gla.ac.uk)
  • As ABF is a commonly used source of material for immunological analysis of Ascaris infection, it could be advisable to look for alternative sources of target antigens that avoid an undue bias towards the Mr 14000 product. (gla.ac.uk)
  • These data suggest that immune serum-activated macrophages can recruit eosinophils to the site of infection, where they act in concert to immobilize tissue-migrating Ascaris larvae. (monash.edu)
  • The combined results of the inoculation of mice with corticosteroids and the examination of the change in profile and number of leukocytes present in bronchoalveolar lavage fluid suggested that the pulmonary inflammatory immune response was not prominently involved in primary protection of mice to A. suum infection in the latter days of infection in the lungs. (tcd.ie)
  • Who is at risk for infection with Ascaris suum ? (cdc.gov)
  • How can I prevent infection with Ascaris suum ? (cdc.gov)
  • Ascaris suum infection was associated with a worm-independent reduction in microbial diversity and altered metabolic potential in the porcine gut microbiome. (nih.gov)
  • In general, Ascaris larval antigen and AP elicited larger intradermal reactions than other antigens in Ascaris -infected animals. (ajtmh.org)
  • Mouse anti-A. suum antisera failed to immunoprecipitate an Mr 14000 component of A. suum larval secretions, which was also found to be the major antigen of adult A. suum body fluid (ABF). (gla.ac.uk)
  • SRIP's with sera from rabbits infected either by A. suum or A. lumbricoides and 125I-labelled IVRS of the larval stages of these parasites followed by examination of the immunoprecipitates by SDS-PAGE, showed that there was complete crossreactivity of a given antiserum of one species and the antigens of the other, including the Mr 17000 antigen of A. lumbricoides. (gla.ac.uk)
  • In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. (unesp.br)
  • Krainson, J, Yerger, L & Ahmed, T 1981, ' Functional depression of airway H 2 -histamine receptors in sheep allergic to Ascaris suum antigen ', American Review of Respiratory Disease , vol. 123, no. 4 II. (elsevier.com)
  • ESP from different A. suum life stages ( in vitro -hatched L3, lung-stage L3, L4, and adult) as well as BF from adult males were analyzed by mass spectrometry. (frontiersin.org)
  • Chromosome aberrations, sister chromatid exchanges (SCE) and cell division kinetics in human lymphocytes exposed in vitro to purified trypsin inhibitor from Ascaris. (semanticscholar.org)
  • Radio-iodination of IVRS from Ascaris species involved material collected from the supernatants of worm cultures in vitro, not necessarily representative of the products of living worms, but could have consisted of worm somatic components. (gla.ac.uk)
  • Gene expression analysis of serum-activated macrophages revealed that CCL24, a potent eosinophil attractant, was the most upregulated gene following culture with A suum larvae in vitro, and human eosinophils displayed even greater ability to adhere to, and trap, A suum larvae. (monash.edu)
  • Ascaris suum metabolic antigens were obtained frorn second and early third stage larvae cultured in vitro in supplemented Eagle's minimum essential medium. (unifesp.br)
  • Os antígenos metabólicos de Ascaris suum foram obtidos de larvas de segundo e de terceiro estágio (precoce) cultivadas in vitro em meio mínimo essencial de Eeagle com suplemento proteico. (unifesp.br)
  • E por último avaliou-se a atividade ovicida in vitro de diferentes concentrações de clamidósporos e de diferentes isolados fúngicos de Pochonia chlamydosporia(VC1 e VC4) sobre ovos de A. suum.Para o estudo epidemiológico,foram avaliados 108.073 fígados de suínos, abatidos durante o período de janeiro de 2011 a junho de 2013, quanto à presença de lesões hepáticas decorrentes da migração larval deA. (ufv.br)
  • Na utilização de clamidósporos do P. chlamydosporia (VC1 e VC4) para a destruição de ovos de A. suum, in vitro, o maior efeito sobre os ovos, ou seja, efeito tipo 2, ocorreu na concentração de 1.000 clamidósporos por placa, apresentando 20.0% (VC4) e 7,33% (VC1) de ovos danificados.Estratégias alternativas e promissoras existem para detecção e controle de A. suum na produção de suínos. (ufv.br)
  • Proanthocyanidins inhibit Ascaris suum glutathione-S-transferase activity and increase susceptibility of larvae to levamisole in vitro. (legumeplus.eu)
  • Kesimpulan yang didapat adalah ekstrak etanol buah nanas (Arunas comosus L.Merr) mempunyai kemampuan sebagai anthelmintik terhadap cacing gelang (Ascaris lumbricoides varietas suum) secara in vivo dan in vitro. (ubaya.ac.id)
  • Twenty-one plants were identified through preliminary field surveys and seven were selected for in vitro anti-helminthic activity against Ascaris suum . (bioline.org.br)
  • How is Ascaris suum spread to humans? (cdc.gov)
  • However, sporadic aberrant A. suum nematode infections have been reported in humans worldwide. (cdc.gov)
  • Therefore, there is a growing need to study the epidemiology of parasites that affect animals and from there to evaluate alternative and sustainable control strategies.The aim of this study was to evaluate alternative control methods to Ascaris suum in swine production. (ufv.br)
  • Schistosoma japonicum and Ascaris suum are considered as the major parasites of human which cause various life threatening diseases such as schistomiasis and ascariasis. (degruyter.com)
  • Ascaris and ascariasis. (degruyter.com)
  • Ascariasis ( Ascaris spp. (cdc.gov)
  • The first studies of radio-iodinated A. suum IVRS showed that the range and number of worm products were different as the parasites developed from the L2 stage to lung stages (L3/4) and that there was considerable, but incomplete stage-specificity of the repertoire of secretions produced. (gla.ac.uk)
  • Vermicomposting was tested for destruction of the model pathogens, helminthic parasites, in an experiment with highly concentrated and viable Ascaris suum (2626±1306 ova/g, 61.6±8.7% viable) inoculated into fecal matter and coir (30:70 ratio) with and without Eisenia fetida worms. (ccsenet.org)
  • Other parasites, such as Necator americanus, Ancylostoma duodenale, and Strongyloides stercoralis, have a similar cycle to Ascaris, with passage of larval forms through the alveolar walls. (medscape.com)
  • Amongst these parasites, soil-transmitted helminths (STHs), including Ancylostoma duodenale and Necator americanus (hookworms), Ascaris sp. (biomedcentral.com)
  • Abstract: Ascaris suum extract (Asc) down-regulates the immune response to ovalbumin (OA) as well as preferentially stimulates a Th2 response to its own components, which is responsible for the suppression of the OA-specific Th1 response. (usp.br)
  • The results of this study indicated no superiority of adult antigens in differentiating Ascaris and Toxocara infections. (ajtmh.org)
  • In Frankfort, Indiana, 87.5% of the sludge samples were positive with Ascaris, Toxocara, Trichuris , and hookworm [ citation needed ] . (bionity.com)
  • Antisera prepared against A. suum malic enzyme reacted to a small extent with the NAD malic enzymes from two free-living nematodes, Panarellus redivivus and Turbatrix aceti. (unt.edu)
  • The mitochondrial genomes of two nematodes, Caenorhabditis elegans and Ascaris suum. (genetics.org)
  • Antisera from A. suum-immunised mice and rats also precipitated components of larval IVRS in SRIP's but different distinct sets of antigens from those recognized by infected rabbits. (gla.ac.uk)
  • In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. (unesp.br)
  • Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis and A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed. (unesp.br)
  • Despite these improvements in fecal matter processing, vermicomposting was found ineffective at reducing Ascaris suum ova concentration and viability. (ccsenet.org)
  • 6] Dmitryjuk, M., Żółtowska, K. (2004): Trehalose catabolism enzymes in tissues of Ascaris suum (Nematoda). (degruyter.com)
  • A globin domain in a neuronal transmembrane receptor of Caenorhabditis elegans and Ascaris suum: molecular modeling and functional properties," JOURNAL OF BIOLOGICAL CHEMISTRY , vol. 290, no. 16, pp. 10336-10352, 2015. (ugent.be)
  • Previous genetic analyses of nematode nAChRs have been confined to Caenorhabditis elegans, which is phylogenetically distinct from Ascaris spp. (ox.ac.uk)
  • The expressed rAs37 was highly recognized by protective immune sera from A. suum Data Availability Statement: All relevant data are within the manuscript and its Supporting egg-infected mice. (deepdyve.com)
  • The recombinant As37 protein (rAs37) was highly recognized by protective immune sera from A. suum infected mice. (deepdyve.com)
  • Additionally, eosinophilic pulmonary infiltrates have appeared in mice challenged with a transnasal Ascaris extract. (medscape.com)
  • A 273-megabase draft genome for A. suum was published in 2011. (wikipedia.org)
  • The A. suum genome has been sequenced by the Davis laboratory at the University of Colorado in collaboration with the Mitreva laboratory at the Genome Institute of Washington University , as described by Wang et al (2012) . (wormbase.org)
  • Additionally, adult A. suum ESP interfered with biofilm formation by Escherichia coli , and caused bacterial agglutination. (frontiersin.org)
  • Muscle, hypodermis and gastrointestinal epithelium from adult female Ascaris lumricoides var. (unt.edu)
  • Haemoglobin of A. suum origin, AsHb, is the most abundant protein in the pseudocoelomic fluid of adult worms and a major excretory⁄secretory protein of larva 3 (L3), L4 and adult worms. (wcgalp.org)
  • These results were further corroborated by a histological examination of the lung tissues which showed that the passage of the larval stages of A. suum through the mouse lungs was associated with a marked inflammatory response in both strains. (tcd.ie)
  • Are Ascaris lumbricoides and Ascaris suum a single species? (wikipedia.org)
  • Codon usage analysis of Ascaris species influence of base and intercodon frequencies on the synonymous codon usage. (degruyter.com)
  • These findings advised against the use of IVRS from Ascaris species in possible serodiagnostic assays or in serological tests to differentiate between A. suum and A. lumbricoides as separate species. (gla.ac.uk)
  • However it might be possible, by raising monoclonal antibodies (McAb) against Ascaris IVRS to find McAb's that differentiate between the Ascaris species and/or provide specificity in serodiagnostic assays. (gla.ac.uk)
  • Other species of the genus Ascaris are parasitic and can cause disease in domestic animals. (bionity.com)
  • The Ascaris trypsin inhibitor (ATI) is a member of a new family of serine protease inhibitors isolated from the helminthic worm Ascaris lumbricoides var suum. (rcsb.org)
  • As the host microbiota represents the direct environment for intestinal helminths and thus a considerable challenge, we studied nematode products, including excretory-secretory products (ESP) and body fluid (BF), of A. suum to determine their antimicrobial activities. (frontiersin.org)
  • Background https://doi.org/10.1371/journal.pntd.0008057 Ascaris lumbricoides is one of the three major soil-transmitted gastrointestinal helminths Editor: David Joseph Diemert, George Washington (STHs) that infect more than 440 million people in the world, ranking this neglected tropical University School of Medicine and Health Sciences, disease among the most common afflictions of people living in poverty. (deepdyve.com)
  • A similar study in the same area showed that 73% of children working on these farms were infected with helminths , particularly Ascaris, probably as a result of exposure to the raw sewage. (bionity.com)
  • However, these mutations are infrequent in the genus Ascaris spp. (bvsalud.org)
  • 1974. Movement of cholestrol and B-sitosterol across the intestine of Ascaris suum. (ou.edu)
  • 1974. Movement of triglycerides and monoglycerides across the intestine of Ascaris summ. (ou.edu)
  • It was purified to homogeneity from the homogenate of Ascaris summ by 2 steps of fractionation using Sephadex G100 and DEAE-Sepnadex A50. (bvsalud.org)
  • Polar zipper sequence in the high-affinity hemoglobin of Ascaris suum: amino acid sequence and structural interpretation. (gersteinlab.org)
  • This entailed mining available transcriptomic and/or genomic sequence datasets for the presence of homologues of known TIMPs, predicting secondary structures of defined protein sequences, systematic phylogenetic analyses and assessment of differential expression of genes encoding putative TIMPs in the developmental stages of A. suum , N. americanus and Schistosoma haematobium which infect the mammalian hosts. (biomedcentral.com)
  • Equilibrium dialysis, product inhibition, and initial velocity studies suggest a rapid-equilibrium random sequential mechanism for the Ascaris suum malic enzyme. (unt.edu)
  • A correlation coefficient of 0.911 was obtained upon comparing the amino acid composition of A. suum and E. coli malic enzymes. (unt.edu)
  • The malic enzyme gene of Ascaris suum was cloned into the vector pTRC99a in two forms encoding alternative amino-termini. (springer.com)
  • Stols L., Kulkarni G., Harris B.G., Donnelly M.I. (1997) Expression of Ascaris suum Malic Enzyme in a Mutant Escherichia coli Allows Production of Succinic Acid from Glucose. (springer.com)
  • Ascaris suum extract was found to contain a mitogenic factor which stimulates human lymphocytes. (semanticscholar.org)
  • High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). (ovid.com)
  • The aim of this study is to establish the anthelmintic potential of M. pruriens and C. schweinfurthii of the crude extract on Pheritima posthuma and partioned crude on two stages of A. suum. (scirp.org)
  • Human macrophages were able to adhere to and trap A suum larvae in the presence of either human or pig serum containing Ascaris-specific antibodies and other factors. (monash.edu)
  • e sobre os parâmetros bioquímicos, hematológicos e histológicos de camundongos tratados por 10 dias consecutivos com diferentes doses do extrato.No terceiro, foram avaliadas soluções saturadas preparadas com quatro sais diferentes (NaCl, MgSO 4 , NaNO 3, ZnSO 4 ) e uma solução de açúcar, na realização da técnica de OPG (técnica de McMaster) em fezes de suínos, contaminadas com ovos de A. suum. (ufv.br)
  • Foi observada a presença do nematoideem animais provenientes de 90% das granjas e a ocorrência total foi 22,4%, média de todo o período experimental.Também foi observado que, durante o período experimental, a ocorrência de A. suum no lume intestinal dos animaisnão apresentou diminuição e os protocolos de everminação não foram eficazes para a diminuição da ocorrência desseshelmintos nas propriedades onde foram empregados . (bvsalud.org)
  • however, the porcine systemic response to A. suum is not entirely elucidated. (wcgalp.org)
  • S. aureus-mediated radio-immunnoprecipitations (SRIP's) of A. suum-immunised rabbit antisera and A. suum IVRS showed that, except for one product from A. suum L3/4 IVRS, all the secretions of A. suum L2 and L3/4 worms are antigenic. (gla.ac.uk)
  • A. suum is in the family Ascarididae, and is one of the oldest associations to mankind. (wikipedia.org)
  • a Multiple transverse sections of A. suum larvae observed in lung tissues with severe, hemorrhagic, fibrinous interstitial pneumonia. (biomedcentral.com)
  • Absence of mutations associated with resistance to benzimidazole in the beta-tubulin gene of Ascaris suum. (bvsalud.org)