Arylsulfatases: Enzymes that catalyze the hydrolysis of a phenol sulfate to yield a phenol and sulfate. Arylsulfatase A, B, and C have been separated. A deficiency of arylsulfatases is one of the causes of metachromatic leukodystrophy (LEUKODYSTROPHY, METACHROMATIC). EC 3.1.6.1.Chondro-4-Sulfatase: An enzyme from the sulfuric ester hydrolase class that breaks down one of the products of the chondroitin lyase II reaction. EC 3.1.6.9.Cerebroside-Sulfatase: An enzyme that catalyzes the hydrolysis of cerebroside 3-sulfate (sulfatide) to yield a cerebroside and inorganic sulfate. A marked deficiency of arylsulfatase A, which is considered the heat-labile component of cerebroside sulfatase, has been demonstrated in all forms of metachromatic leukodystrophy (LEUKODYSTROPHY, METACHROMATIC). EC 3.1.6.8.SulfatasesLeukodystrophy, Metachromatic: An autosomal recessive metabolic disease caused by a deficiency of CEREBROSIDE-SULFATASE leading to intralysosomal accumulation of cerebroside sulfate (SULFOGLYCOSPHINGOLIPIDS) in the nervous system and other organs. Pathological features include diffuse demyelination, and metachromatically-staining granules in many cell types such as the GLIAL CELLS. There are several allelic and nonallelic forms with a variety of neurological symptoms.Cerebrosides: Neutral glycosphingolipids that contain a monosaccharide, normally glucose or galactose, in 1-ortho-beta-glycosidic linkage with the primary alcohol of an N-acyl sphingoid (ceramide). In plants the monosaccharide is normally glucose and the sphingoid usually phytosphingosine. In animals, the monosaccharide is usually galactose, though this may vary with the tissue and the sphingoid is usually sphingosine or dihydrosphingosine. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1st ed)Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Central Nervous System Diseases: Diseases of any component of the brain (including the cerebral hemispheres, diencephalon, brain stem, and cerebellum) or the spinal cord.Dictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.N-Acetylgalactosamine-4-Sulfatase: An arylsulfatase that catalyzes the hydrolysis of the 4-sulfate groups of the N-acetyl-D-galactosamine 4-sulfate units of chondroitin sulfate and dermatan sulfate. A deficiency of this enzyme is responsible for the inherited lysosomal disease, Maroteaux-Lamy syndrome (MUCOPOLYSACCHARIDOSIS VI). EC 3.1.6.12.Mucopolysaccharidosis VI: Mucopolysaccharidosis with excessive CHONDROITIN SULFATE B in urine, characterized by dwarfism and deafness. It is caused by a deficiency of N-ACETYLGALACTOSAMINE-4-SULFATASE (arylsulfatase B).Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Metals: Electropositive chemical elements characterized by ductility, malleability, luster, and conductance of heat and electricity. They can replace the hydrogen of an acid and form bases with hydroxyl radicals. (Grant & Hackh's Chemical Dictionary, 5th ed)Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.HandbooksBlood Specimen Collection: The taking of a blood sample to determine its character as a whole, to identify levels of its component cells, chemicals, gases, or other constituents, to perform pathological examination, etc.Leukodystrophy, Globoid Cell: An autosomal recessive metabolic disorder caused by a deficiency of GALACTOSYLCERAMIDASE leading to intralysosomal accumulation of galactolipids such as GALACTOSYLCERAMIDES and PSYCHOSINE. It is characterized by demyelination associated with large multinucleated globoid cells, predominantly involving the white matter of the central nervous system. The loss of MYELIN disrupts normal conduction of nerve impulses.Sulfoglycosphingolipids: GLYCOSPHINGOLIPIDS with a sulfate group esterified to one of the sugar groups.Pseudomonas aeruginosa: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.Pseudomonas Infections: Infections with bacteria of the genus PSEUDOMONAS.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.Hexosaminidase A: A mammalian beta-hexosaminidase isoform that is a heteromeric protein comprized of both hexosaminidase alpha and hexosaminidase beta subunits. Deficiency of hexosaminidase A due to mutations in the gene encoding the hexosaminidase alpha subunit is a case of TAY-SACHS DISEASE. Deficiency of hexosaminidase A and HEXOSAMINIDASE B due to mutations in the gene encoding the hexosaminidase beta subunit is a case of SANDHOFF DISEASE.beta-Mannosidosis: An inborn error of metabolism marked by a defect in the lysosomal isoform of BETA-MANNOSIDASE that results in lysosomal accumulation of mannose-rich intermediate metabolites containing 1,4-beta linkages. The human disease occurs through autosomal recessive inheritance and manifests itself with variety of symptoms that depend upon the type of gene mutation.Ion Pumps: A general class of integral membrane proteins that transport ions across a membrane against an electrochemical gradient.Phosphoric Monoester Hydrolases: A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate. EC 3.1.3.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Cadmium: An element with atomic symbol Cd, atomic number 48, and atomic weight 114. It is a metal and ingestion will lead to CADMIUM POISONING.Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.Staphylococcus saprophyticus: A species of gram-positive bacteria in the family STAPHYLOCOCCACEAE. It commonly causes urinary tract infections in humans.Carbohydrate Epimerases: Enzymes that catalyze the epimerization of chiral centers within carbohydrates or their derivatives. EC 5.1.3.GABA-B Receptor Agonists: Endogenous compounds and drugs that bind to and activate GABA-B RECEPTORS.Mannosephosphates: Phosphoric acid esters of mannose.Receptor, IGF Type 2: A receptor that is specific for IGF-II and mannose-6-phosphate. The receptor is a 250-kDa single chain polypeptide which is unrelated in structure to the type 1 IGF receptor (RECEPTOR, IGF TYPE 1) and does not have a tyrosine kinase domain.Mucolipidoses: A group of inherited metabolic diseases characterized by the accumulation of excessive amounts of acid mucopolysaccharides, sphingolipids, and/or glycolipids in visceral and mesenchymal cells. Abnormal amounts of sphingolipids or glycolipids are present in neural tissue. INTELLECTUAL DISABILITY and skeletal changes, most notably dysostosis multiplex, occur frequently. (From Joynt, Clinical Neurology, 1992, Ch56, pp36-7)Saposins: A group of four homologous sphingolipid activator proteins that are formed from proteolytic cleavage of a common protein precursor molecule referred to as prosaposin.Arsenite Transporting ATPases: Efflux pumps that use the energy of ATP hydrolysis to pump arsenite across a membrane. They are primarily found in prokaryotic organisms, where they play a role in protection against excess intracellular levels of arsenite ions.Antimony: A metallic element that has the atomic symbol Sb, atomic number 51, and atomic weight 121.75. It is used as a metal alloy and as medicinal and poisonous salts. It is toxic and an irritant to the skin and the mucous membranes.Arsenites: Inorganic salts or organic esters of arsenious acid.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Cervical Plexus: A network of nerve fibers originating in the upper four CERVICAL SPINAL CORD segments. The cervical plexus distributes cutaneous nerves to parts of the neck, shoulders, and back of the head. It also distributes motor fibers to muscles of the cervical SPINAL COLUMN, infrahyoid muscles, and the DIAPHRAGM.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Infant, Postmature: An infant born at or after 42 weeks of gestation.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Lysosomes: A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)

Enzymatic and immunological characterization of the Mycobacterium fortuitum complex. (1/249)

The arylsulfatase isozymes of Mycobacterium fortuitum, M. peregrinum, M. chelonei subsp. chelonei, and M. chelonei subsp. abscessus were examined to determine the isozymal and immunological relationship among the members of the M. fortuitum complex. Cell extracts were subjected to electrophoresis on agarose and polyacrylamide gel, and arylsulfatase activity was localized using beta-naphthyl sulfate as substrate. Unique zymograms were produced for M. fortuitum, M. peregrinum, and M. chelonei which were characteristic for each species. The immunological relationship among the sulfatases was assayed by using immunodiffusion and immunoelectrophoresis followed by sulfatase staining for the enzyme. One of the isozymes of M. fortuitum and M. peregrinum cross-reacted, showing immunological identity. Antisera to sulfatases of M. fortuitum and M. peregrinum did not react with sulfatases of M. chelonei. The characterization of sulfatase isozymes in extracts of organisms in the M. fortuitum complex suggests the division of the M. fortuitum complex into two species, M. fortuitum and M. chelonei, with subspecies designations.  (+info)

Induction of selected lipid metabolic enzymes and differentiation-linked structural proteins by air exposure in fetal rat skin explants. (2/249)

The epidermal permeability barrier of premature infants matures rapidly following birth. Previous studies suggest that air exposure could contribute to this acceleration, because: (i) development of a structurally and functionally mature barrier accelerates when fetal rat skin explants are incubated at an air-medium interface, and (ii) occlusion with a water-impermeable membrane prevents this acceleration. To investigate further the effects of air exposure on epidermal barrier ontogenesis, we compared the activities of several key enzymes of lipid metabolism and gene expression of protein markers of epidermal differentiation in fetal rat skin explants grown immersed versus air exposed. The rate-limiting enzymes of cholesterol (HMG CoA reductase) and ceramide (serine palmitoyl transferase) synthesis were not affected. In contrast, the normal developmental increases in activities of glucosylceramide synthase and cholesterol sulfotransferase, responsible for the synthesis of glucosylceramides and cholesterol sulfate, respectively, were accelerated further by air exposure. Additionally, two enzymes required for the final stages of barrier maturation and essential for normal stratum corneum function, beta-glucocerebrosidase, which converts glucosylceramide to ceramide, and steroid sulfatase, which desulfates cholesterol sulfate, also increased with air exposure. Furthermore, filaggrin and loricrin mRNA levels, and filaggrin, loricrin, and involucrin protein levels all increased with air exposure. Finally, occlusion with a water-impermeable membrane prevented both the air-exposure-induced increase in lipid enzyme activity, and the expression of loricrin, filaggrin, and involucrin. Thus, air exposure stimulates selected lipid metabolic enzymes and the gene expression of key structural proteins in fetal epidermis, providing a biochemical basis for air-induced acceleration of permeability barrier maturation in premature infants.  (+info)

HpEts, an ets-related transcription factor implicated in primary mesenchyme cell differentiation in the sea urchin embryo. (3/249)

The mechanism of micromere specification is one of the central issues in sea urchin development. In this study we have identified a sea urchin homologue of ets 1 + 2. HpEts, which is maternally expressed ubiquitously during the cleavage stage and which expression becomes restricted to the skeletogenic primary mesenchyme cells (PMC) after the hatching blastula stage. The overexpression of HpEts by mRNA injection into fertilized eggs alters the cell fate of non-PMC to migratory PMC. HpEts induces the expression of a PMC-specific spicule matrix protein, SM50, but suppresses of aboral ectoderm-specific arylsulfatase and endoderm-specific HpEndo16. The overexpression of dominant negative delta HpEts which lacks the N terminal domain, in contrast, specifically represses SM50 expression and development of the spicule. In the upstream region of the SM50 gene there exists an ets binding site that functions as a positive cis-regulatory element. The results suggest that HpEts plays a key role in the differentiation of PMCs in sea urchin embryogenesis.  (+info)

An Asn > Lys substitution in saposin B involving a conserved amino acidic residue and leading to the loss of the single N-glycosylation site in a patient with metachromatic leukodystrophy and normal arylsulphatase A activity. (4/249)

Sphingolipid activator proteins are small glycoproteins required for the degradation of sphingolipids by specific lysosomal hydrolases. Four of them, called saposins, are encoded by the prosaposin gene, the product of which is proteolytically cleaved into the four mature saposin proteins (saposins A, B, C, D). One of these, saposin B, is necessary in the hydrolysis of sulphatide by arylsulphatase A where it presents the solubilised substrate to the enzyme. As an alternative to arylsulphatase A deficiency, deficiency of saposin B causes metachromatic leukodystrophy. We identified a previously undescribed mutation (N215K) in the prosaposin gene of a patient with metachromatic leukodystrophy but with normal arylsulphatase A activity and elevated sulphatide in urine. The mutation involves a highly conserved amino acidic residue and abolishes the only N-glycosylation site of saposin B.  (+info)

Induction of coproporphyrinogen oxidase in Chlamydomonas chloroplasts occurs via transcriptional regulation of Cpx1 mediated by copper response elements and increased translation from a copper deficiency-specific form of the transcript. (5/249)

Coproporphyrinogen III oxidase, encoded by a single nuclear gene in Chlamydomonas reinhardtii, produces three distinct transcripts. One of these transcripts is greatly induced in copper-deficient cells by transcriptional activation, whereas the other forms are copper-insensitive. The induced form of the transcript was expressed coordinately with the cytochrome c6-encoding (Cyc6) gene, which is known to be transcriptionally regulated in copper-deficient cells. The sequence GTAC, which forms the core of a copper response element associated with the Cyc6 gene, is also essential for induction of the Cpx1 gene, suggesting that both are targets of the same signal transduction pathway. The constitutive and induced Cpx1 transcripts have the same half-lives in vivo, and all encode the same polypeptide with a chloroplast-targeting transit sequence, but the shortest one representing the induced form is a 2-4-fold better template for translation than are either of the constitutive forms. The enzyme remains localized to a soluble compartment in the chloroplast even in induced cells, and its abundance is not affected when the tetrapyrrole pathway is manipulated either genetically or by gabaculine treatment.  (+info)

A novel mucin-sulphatase activity found in Burkholderia cepacia and Pseudomonas aeruginosa. (6/249)

Lung infections due to Burkholderia cepacia and Pseudomonas aeruginosa in patients with cystic fibrosis (CF) are common, are associated with respiratory morbidity and are a cause of mortality. Respiratory mucin in CF patients is highly sulphated, which increases its resistance to bacterial degradation. Desulphation increases the susceptibility of mucin to degradation by bacterial glycosidases and proteinases, and subsequent deglycosylation may facilitate bacterial colonisation by increasing available substrates and binding sites. This study determined whether clinical and environmental strains of B. cepacia and P. aeruginosa had the ability to desulphate mucin. Mucin-sulphatase activity was tested by incubating bacterial cell suspensions with 35S-sulphated mucins purified from LS174T and HT29-MTX human colon carcinoma cell lines. These mucins were also used to test for differences in substrate specificities. Mucin-sulphatase activity was detected in all nine B. cepacia strains and in four of six P. aeruginosa strains. There was strain variability in the level of mucin-sulphatase activity. Aryl-sulphatase activities of Pseudomonas isolates (determined with methylumbelliferyl sulphate) were c. 20-fold higher than those of B. cepacia strains, and were independent of mucin-sulphatase activity. This is the first report to demonstrate desulphation of mucin by B. cepacia and P. aeruginosa. It is concluded that B. cepacia and P. aeruginosa produce one or more cell-bound glycosulphatase(s), in addition to aryl-sulphatase activity. Mucin-sulphatase activity of B. cepacia and P. aeruginosa may contribute to their association with airway infections in patients with cystic fibrosis.  (+info)

Sac3, an Snf1-like serine/threonine kinase that positively and negatively regulates the responses of Chlamydomonas to sulfur limitation. (7/249)

The Sac3 gene product of Chlamydomonas positively and negatively regulates the responses of the cell to sulfur limitation. In wild-type cells, arylsulfatase activity is detected only during sulfur limitation. The sac3 mutant expresses arylsulfatase activity even when grown in nutrient-replete medium, which suggests that the Sac3 protein has a negative effect on the induction of arylsulfatase activity. In contrast to its effect on arylsulfatase activity, Sac3 positively regulates the high-affinity sulfate transport system-the sac3 mutant is unable to fully induce high-affinity sulfate transport during sulfur limitation. We have complemented the sac3 mutant and cloned a cDNA copy of the Sac3 gene. The deduced amino acid sequence of the Sac3 gene product is similar to the catalytic domain of the yeast Snf1 family of serine/threonine kinases and is therefore classified as a Snf1-related kinase (SnRK). Specifically, Sac3 falls within the SnRK2 subfamily of kinases from vascular plants. In addition to the 11 subdomains common to Snf1-like serine/threonine kinases, Sac3 and the plant kinases have two additional subdomains and a highly acidic C-terminal region. The role of Sac3 in the signal transduction system that regulates the responses of Chlamydomonas to sulfur limitation is discussed.  (+info)

Transposition of SRY into the ancestral pseudoautosomal region creates a new pseudoautosomal boundary in a progenitor of simian primates. (8/249)

We have isolated the prosimian lemur homologues for STS and SRY. FISH unambiguously co-localized STS with SHOX, IL3RA, ANT3 and PRK into the meiotic X-Y pairing region (PAR) of lemurs. In contrast to the close proximity of SRY to the pseudoautosomal boundary (PAB) on the Y chromosome in simian primates, SRY maps distant from the PAR in lemurs. Most interestingly, we were able to determine a DNA sequence divergence of 12.5% between the human and lemur SRY HMG box. This divergence directs to a 52 million year period of separate evolution of human and lemur SRY genes. Phylogenetically, this time period falls in between the times that prosimians and New World monkeys branched from the human lineage. Thus, we conclude that approximately 52 million years ago a transposition of SRY into the ancestral eutherian PAR distal to STS and PRK defined a new PAB in a simian progenitor. By this event, STS and PRK, amongst other genes, were excluded from the X-Y crossover process and thus became susceptible to rearrangements and/or deterioration on the Y chromosome in simian primates.  (+info)

Arylsulfatases: Enzymes that catalyze the hydrolysis of a phenol sulfate to yield a phenol and sulfate. Arylsulfatase A, B, and C have been separated. A deficiency of arylsulfatases is one of the causes of metachromatic leukodystrophy (LEUKODYSTROPHY, METACHROMATIC). EC 3.1.6.1.
Define Arylsulfatase E. Arylsulfatase E synonyms, Arylsulfatase E pronunciation, Arylsulfatase E translation, English dictionary definition of Arylsulfatase E. n. Chiefly British Slang Variant of ass2. or n 1. the buttocks 2. the anus 3. a stupid person; fool 4. sexual intercourse 5. Austral effrontery; cheek 6....
The pig endometrial arylsulphatase A was purified 3322-fold to a specific activity of 150 mumol/min per mg. The purification involved (NH4)2SO4 fractionation, chromatography on concanavalin A-Sepharose and DEAE-Sepharose, gel filtrations on Sephadex G-200 at pH 7.4 and 5, and a new preparative gel-electrophoresis technique. The homogeneous enzyme is a glycoprotein containing 20% carbohydrate. The purified enzyme has Mr about 120 000 and it contains subunits of Mr 63 000. The pig endometrial arylsulphatase A shows many properties in common with those of arylsulphatases A purified from other sources. The similarities include their low isoelectric points, the anomalous time-activity relationships, multi-pH optima, inhibition by SO3(2-), SO4(2-), phosphate ions, metal ions and nucleoside phosphates, pH- and ionic-strength-dependent polymerization and amino acid composition. ...
Bouchet, P. (2013). Charonia rubicunda (Perry, 1811). In: MolluscaBase (2017). Accessed through: Odido, M.; Appeltans, W.; BelHassen, M.; Mussai, P.; Nsiangango, S.E.; Vandepitte, L.; Wambiji, N.; Zamouri, N. Jiddou, A.M. (Eds) (2017). African Register of Marine Species at http://marinespecies.org/afremas/aphia.php?p=taxdetails&id=717009 on 2017-12- ...
Learn why horse owners should not be alarmed with a diagnosis of Lampas and why feeding a soft, moist ration is sometimes necessary if the horse has difficulty eating for a short time period.
Perhaps the most powerful home-made Tesla Coil in Singapore. Includes construction details, how-to, schematics, photographs and videos!
Arylsulfatase A antibody [N2C2], Internal (arylsulfatase A) for WB. Anti-Arylsulfatase A pAb (GTX106155) is tested in Human samples. 100% Ab-Assurance.
The relative activities of arylsulphatases A and B were measured in rat liver parenchymal and non-parenchymal cells, in peritoneal macrophages and in a number of rat tissues. Although absolute values cannot be obtained, it was shown that the arylsulphatase B/arylsulphatase A activity ratio is much higher in non-parenchymal cells than in parenchymal cells. The ratios in adrenals, brain and testis are very similar to each other but differ from those found in spleen, kidney and liver. These ratio variations may be caused by alterations in the activity of the B enzyme rather than the A enzyme. The relatively high B enzyme/A enzyme ratios in all rat tissues explains why the method devised for the independent assay of human arylsulphatases A and B cannot be employed with rat tissues.. ...
ARSB : Arylsulfatase B (ARSB) can be measured with nitrocatechol sulfate as the substrate. The conditions established are such that arylsulfatase A activity is minimal and its residual activity can be accounted for and subtracted from the activity of ARSB.(Baum H, Dodgson KS, Spencer B: Assay of arylsulfatases A and B in human urine. Clin Chim Acta 1959;4:453-455)
Steroid sulfatase兔多克隆抗体(ab62219)可与人样本反应并经WB, IHC实验严格验证,被1篇文献引用。所有产品均提供质保服务,中国75%以上现货。
The new paper, entitled Structure-activity relationship of the clinical steroid sulfatase inhibitor Irosustat (STX64, BN83495), has appeared in the November issue of the journal and describes how the research team has modified the chemical structure of the drug to explore the effects on biological activity.. Picture showing how the drug molecule (right) interacts with the steroid sulfatase enzyme (left).. Referee reports on the paper were so strong that the journal ChemMedChem accorded it coveted VIP status and also invited the authors to design a cover feature for the publication.. Professor Potter, with colleagues Dr Lawrence Woo and Dr Mark Thomas of the Department of Pharmacy & Pharmacology, designed an imaginative cover feature illustrating the drug molecule flanked by renderings of the target enzyme, all superimposed upon a false colour staining of the target protein in malignant breast cancer cells.. The publisher Wiley has also issued a feature on this paper in its own Chem Views ...
The study was conducted in Adama town, on carcass samples collected from the Arsi breed of cattle with the objective of evaluating beef microbiological qualities with sta..
G. DUBOIS, J. C. TURPIN, N. BAUMANN; Electrophoretic Characterization of A and B Isoenzymes of Arylsulfatase. Biochem Soc Trans 1 April 1974; 2 (2): 256. doi: https://doi.org/10.1042/bst0020256. Download citation file:. ...
1HDH: 1.3 A Structure of Arylsulfatase from Pseudomonas Aeruginosa Establishes the Catalytic Mechanism of Sulfate Ester Cleavage in the Sulfatase Family.
New Jersey Superior Court, Appellate Division,Decided: October 29, 1985,King,Joseph Scalia argued the cause for appellant (Berry, Kagan, Privetera & Sahradnik, attorneys; Roberta Torre Quinn, on the brief).,JOHN ARSI, SR., PETITIONER-RESPONDENT, v. OCEAN COUNTY ROAD DEPARTMENT, RESPONDENT-APPELLANT
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Finally tried a real mutant. moar pics on my blog. https://2.bp.blogspot.com/-KxXU_60N1ms/XOGU1np0iII/AAAAAAAABWs/HZki74u_FA0bfuXXBu72P9aV_qFaSPaaQCLcBGAs/s1600/Slide2.JPG https://2.bp.blogspot.com/-BCIdTRNkivg/XOGVXkU9O6I/AAAAAAAABXk/Ebn20X6XHwMtgZMA6ImLM2xLYIQN3sapwCLcBGAs/s1600/Slide6.JPG
The development of potent steroid sulfatase inhibitors is an important new therapeutic strategy for the treatment of postmenopausal women with breast cancer. A series of tricyclic coumarin sulfamates were synthesized, and their inhibitory properties were examined in vitro and in vivo. In a placental microsomal assay system, 667 COUMATE emerged as the most potent inhibitor with an IC50 of 8 nM. Administration of a single dose (10 mg/kg, p.o.) of 667 COUMATE inhibited rat liver estrone sulfatase activity by 93%. 667 COUMATE was devoid of estrogenicity, as indicated by its failure to stimulate the growth of uteri in ovariectomized rats. In vivo, estrone sulfate-stimulated growth of uteri in ovariectomized rats was inhibited by 667 COUMATE. Using the nitrosomethylurea-induced mammary tumor model, we found that 667 COUMATE caused regression of estrone sulfate-stimulated tumor growth in a dose-dependent manner. The identification of 667 COUMATE as a potent steroid sulfatase inhibitor will enable the
Arylsulfatase A (or cerebroside-sulfatase) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate into cerebroside and sulfate. In humans, arylsulfatase A is encoded by the ARSA gene. A deficiency is associated with metachromatic leukodystrophy, an autosomal recessive disease. Arylsulfatase A is inhibited by phosphate, which forms a covalent bond with the active site 3-oxoalanine. GRCh38: Ensembl release 89: ENSG00000100299 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000022620 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Stein C, Gieselmann V, Kreysing J, Schmidt B, Pohlmann R, Waheed A, Meyer HE, OBrien JS, von Figura K (January 1989). "Cloning and expression of human arylsulfatase A". J. Biol. Chem. 264 (2): 1252-9. PMID 2562955. Matzner U, Herbst E, Hedayati KK, Lüllmann-Rauch R, Wessig C, Schröder S, Eistrup C, Möller C, Fogh J, Gieselmann V (May 2005). "Enzyme replacement improves nervous system pathology and function in a mouse ...
A series of novel D-ring modified derivatives of estrone was synthesized and tested as inhibitors of steroid sulfatase (STS). The steroidal D-ring was cleaved via an iodoform reaction and thermal condensation of the resulting marrianolic acid derivative gave 16,17-seco-estra-1,3,5(10)-triene-16,17-imide derivatives, where a piperidinedione moiety is in place of the D-ring. This synthetic approach was found to give a higher overall yield than the literature method of Beckmann rearrangement. A range of alkyl side chains have been introduced on the nitrogen atom of the imido-ring and the corresponding 3-O-sulfamates synthesized. The new D-ring modified estrone derivatives bearing a propyl (39) and a 1-pyridin-3-ylmethyl (46) moiety had IC(50) values of 1 nM when tested in placental microsomes for the inhibition of STS. These compounds are therefore up to 18-fold more potent than EMATE, the very first highly potent irreversible steroidal STS inhibitor.
Although arylsulfatase A pseudodeficiency is characterized as a disease, about 1-2% of any population of clinically healthy people have two copies of "the" ARSA pseudodeficiency allele, identified by rs6151429. This does lead to low levels of arylsulfatase (ARSA).[PMID 1678251 ...
Pseudomonas aeruginosa arylsulfatase (PAS) is a bacterial sulfatase capable ofhydrolyzing a range of sulfate esters. Recently, it has been demonstrated to also show very high proficiency for phosphate ester hydrolysis. Such proficient catalytic promiscuity is significant, as promiscuity has been suggested to play an important role in enzyme evolution. Additionally, a comparative study of the hydrolyses of the p-nitrophenyl phosphate and sulfate monoesters in aqueous solution has demonstrated that despite superficial similarities, the two reactions proceed through markedly different transition states with very different solvation effects, indicating that the requirements for the efficient catalysis of the two reactions by an enzyme will also be very different (and yet they are both catalyzed by thesame active site). This work explores the promiscuous phosphomonoesterase activity ofPAS. Specifically, we have investigated the identity of the most likely base for the initial activation of the ...
Updated: 2019/03/07 13:47:37. Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.. Vacutainer® and/or Microtainer® are registered trademarks of Becton, Dickinson & Company.. ...
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PubMedID: 27268575 | The systematic regulation of oyster CgIL17-1 and CgIL17-5 in response to air exposure. | Developmental and comparative immunology | 10/1/2016
Dodgson, K.S., Spencer, B. and Williams, K. (1956). „Studies on sulphatases. 13. The hydrolysis of substituted phenyl sulphates by the arylsulphatase of Alcaligenes metacaligenes". Biochem. J. 64: 216-221. PMID 13363831 ...
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With no concrete solutions on the table regarding the much-feared fiscal cliff, it wasnt terribly surprising to see equity markets take a beating last week. Profit-taking pressures have been a dominant ...
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TY - JOUR. T1 - The phenotype spectrum of X-linked ichthyosis identified by chromosomal microarray. AU - Hand, Jennifer L.. AU - Runke, Cassandra K.. AU - Hodge, Jennelle C.. PY - 2015/4/1. Y1 - 2015/4/1. N2 - Background Steroid sulfatase (STS) gene disruption causes X-linked ichthyosis (XLI). Interrogating the entire genome through chromosomal microarray (CMA), a test primarily used to screen patients with noncutaneous congenital anomalies, may detect STS deletions incidentally. Objective We sought to determine the variability of skin features associated with STS deletions diagnosed through CMA and to compare these findings with XLI cases reported in the literature and recognized in a dermatology clinic. Methods Male patients with an STS deletion were identified from 23,172 consecutive postnatal blood samples tested with CMA at Mayo Clinic. A comparison group of male patients with biochemically confirmed XLI was ascertained in the dermatology clinic. The available patient medical records, skin ...
On October 6, 2016 results were published in the PLOS journal regarding a recent X-Linked personality survey. The article is titled Behavioural and Psychiatric Phenotypes in Men and Boys with X-Linked Ichthyosis: Evidence from a Worldwide Online Survey. An abstract of the results can be found at: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0164417. FIRST MSAB member, Dr. Jennifer Hand of the Mayo Clinic adds, "This study is interesting but has important limitations to consider. Many of the individuals studied were collected from within patient support groups. This means that the study is likely biased towards individuals who had severe enough health concerns that they or their families sought out help from a support group. That is, this study probably overemphasizes the behavioral problems associated with XLI. A more meaningful result would come from a random sample of individuals found to have the gene deletion that causes X-linked ichthyosis.". ...
CR: Disappearance of all known disease & no new sites / disease related symptoms confirmed at least 12 weeks after initial documentation. Disappearance of all non-target lesions. Normalization of tumor marker level confirmed at least 12 weeks after initial documentation.. PR: Minimum 30% decrease in sum of the longest diameters of target lesions, taking as a reference the baseline sum of the longest diameters confirmed at least 12 weeks after initial documentation. PR is also recorded when all measurable disease has completely disappeared, but a non-measurable component (i.e., ascites) is still present but not progressing. As well as persistence of one or more non-target lesion(s) and/or maintenance of tumor marker level above normal limits.. RECIST defines SD for target lesions as neither sufficient shrinkage to qualify for partial response nor sufficient increase to qualify for progressive disease, no occurrence of progression disease for non-target lesions, and no new lesions. ...
Jiang M, Klein M, Zanger UM, Mohammad MK, Cave MC, Gaikwad NW, Dias NJ, Selcer KW, Guo Y, He J, Zhang X, Shen Q, Qin W, Li J, Li S, Xie W. Inflammatory regulation of steroid sulfatase: A novel mechanism to control estrogen homeostasis and inflammation in chronic liver disease. J Hepatol. 2016 Jan;64(1):44-52. doi: 10.1016/j.jhep.2015.07.022. Epub 2015 Jul 26. PubMed PMID: 26220752; PubMed Central PMCID: PMC4691383 ...
Random Tn917 mutagenesis of Bacillus subtilis followed by selection of lipoic acid auxotrophs led to the isolation of the cysH gene. The gene was sequenced and found to encode a phosphoadenylylsulfate sulfotransferase with a molecular mass of 27 kDa. Expression of lacZ fused to the cysH promoter was repressed by cysteine and sulfide and induced by sulfur limitation, indicating that cysH is controlled at the level of transcription. ...
1114 Recent studies have demonstrated the multi-mechanism anti-tumor effects of 2-substituted estradiol bis-sulfamates and highlighted a number of potential advantages that such compounds possess relative to the corresponding estradiols, which include 2-methoxyestradiol (2-MeOE2, Panzem ™) a drug in Phase II clinical trials. 2-Methoxy- (2-MeOE2bisMATE, STX140, 1) and 2-ethyl-estradiol bis-sulfamate (2-EtE2bisMATE, STX243, 2) display potent anti-proliferative and anti-angiogenic activity and also function as inhibitors of steroid sulphatase (STS), a clinical target for the treatment of hormone dependent breast cancer. In order to generate novel agents with anti-proliferative activity against human breast cancer cells we have synthesised a number of 2-substituted estradiol 3-O-sulfamate analogues with oxygenated side chains tethered at C-17 as bio-isosteric replacements of the 17-O-sulfamate group of the bisMATEs. The 17β-(2-hydroxyethyl) compound 3 in which the hydroxyl group can function as ...
If you have used this database, please ensure that you acknowledge this most recent Pseudomonas Genome Database publication rather than just the website URL. Thank you!. Winsor GL, Griffiths EJ, Lo R, Dhillon BK, Shay JA, Brinkman FS (2016 ...
Extracted Text: VOL. XLI, No. 3 WHOLE No. 219 MAY/JUNE 2002 CONFEDERATE & SOUTHERN STATES SPECIAL ISSUE C tl...
ARS-853 is the first direct KRAS inhibitor shown to selectively inhibit KRAS in cells with potency in the range of a drug candidate. Previously reported KRAS inhibitors have either shown insufficient potency for detailed cellular characterization (2, 3, 6), or exhibit dramatic deviations in potency across assays (4). The most potent covalent KRASG12C ligand reported previously (Compound 12) exhibited promising in vitro properties, but its cellular effects were less clear (6). Here, we demonstrate that Compound 12 is not capable of engaging KRASG12C in cells even at a relatively high dose and long incubation (100 μmol/L for 6 hours). However, by improving upon the groundbreaking approach of targeting the Switch II pocket of KRASG12C with covalent ligands, we have successfully identified a covalent inhibitor that demonstrates consistent low micromolar activity from biochemical and cellular engagement to KRASG12C activation, downstream signaling, and cell survival. Further, we have found that ...
Nadar, Venkadesh Sarkarai; Yoshinaga, Masafumi; Pawitwar, Shashank S et al. (2016) Structure of the ArsI C-As Lyase: Insights into the Mechanism of Degradation of Organoarsenical Herbicides and Growth Promoters. J Mol Biol 428:2462-73 ...
A middle-aged woman had suffered for a few months from vomiting, pain in the abdomen, and loss of flesh. Examination showed a large tumour in the umbilical region and left hypochondrium, which was som...
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TY - JOUR. T1 - Differential expression of steroid sulphatase locus on active and inactive human X chromosome. AU - Migeon, Barbara R. AU - Shapiro, Larry J.. AU - Norum, Robert A.. AU - Mohandas, Thuluvancheri. AU - Axelman, Joyce. AU - Dabora, Rebecca L.. PY - 1982. Y1 - 1982. N2 - The X chromosome in mammalian somatic cells is subject to unique regulation - usually genes on a single X chromosome are expressed while those on other X chromosomes are inactivated1. The X-locus for steroid sulphatase (STS; EC 3.1.6.2), the microsomal enzyme that catalyses the hydrolysis of various 3β-hydroxysteroid sulphates, is exceptional because it seems to escape inactivation. Evidence for this comes from fibroblast clones in females heterozygous for mutations that result in a severe deficiency of this enzyme in affected males; all clones from these heterozygotes have STS activity, and enzyme-deficient clones that are expected if the locus were subject to inactivation2, have not been found3. Further evidence ...
X-linked ichthyosis is a relatively common genetic disorder of keratinization. It is the second most common type of ichthyosis after vulgaris. The incidence is estimated to be between 1 in 2,000 and...
Multiplex polymerase chain reaction (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously (as if performing many separate PCR reactions all together in one reaction). This process amplifies DNA in samples using multiple primers and a temperature-mediated DNA polymerase in a thermal cycler. The primer design for all primers pairs has to be optimized so that all primer pairs can work at the same annealing temperature during PCR. Multiplex-PCR was first described in 1988 as a method to detect deletions in the dystrophin gene. It has also been used with the steroid sulfatase gene. In 2008, multiplex-PCR was used for analysis of microsatellites and SNPs. Multiplex-PCR consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple sequences at once, additional information may be gained from a single test run that otherwise would ...
This trial will explore the safety and efficacy of BN83485 compared to Megestrol Acetate (MA) on progression free survival (PFS) in post menopausal pati
Lai, Rasha Elgag Elsadig, Karin Reimann, Cheng Har Yip and Leslie (2001) Inhibition of oestrone sulphatase activity in the MDA-MB-231 Breast Cancer Cell Line by Breast Cyst Fluid from Malaysian Women. Anticancer Research, 21. pp. 2693-2696. ...
1AUK: Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis.
Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sul
Abcam provides specific protocols for Anti-Iduronate 2 sulfatase antibody (ab85701) : Western blot protocols, Immunohistochemistry protocols
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Creed Pearsons Scientology doubt formula - The Biggest Secrets of Scientology - A (25 year member) Discovering Scientology s Greatest Secret and Why I was Silenced, by Creed J. Pearson May 2005 To those who care: Before I begin this rather lengthy expose , I would like the reader to read the affirmations of L. Ron Hubbard written in 1947. These are also known as the admissions. It was only after reading these and continuing my research that I was able to finally come out of doubt for real. These are not OT levels and are not confidential. To read these, see http://www.gerryarmstrong.org/50grand/writings/ars/ars-2000-03-11.html.
Accepted name: iduronate-2-sulfatase. Reaction: Hydrolysis of the 2-sulfate groups of the L-iduronate 2-sulfate units of dermatan sulfate, heparan sulfate and heparin. Other name(s): chondroitinsulfatase; idurono-2-sulfatase; iduronide-2-sulfate sulfatase; L-iduronosulfatase; L-idurono sulfate sulfatase; iduronate sulfatase; sulfo-L-iduronate sulfatase; L-iduronate 2-sulfate sulfatase; sulfoiduronate sulfohydrolase; 2-sulfo-L-iduronate 2-sulfatase; iduronate-2-sulfate sulfatase; iduronate sulfate sulfatase. Systematic name: L-iduronate-2-sulfate 2-sulfohydrolase. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 50936-59-9. References:. 1. Archer, I.M., Harper, P.S. and Wusteman, F.S. Multiple forms of iduronate 2-sulphate sulphatase in human tissues and body fluids. Biochim. Biophys. Acta 708 (1982) 134-140. [PMID: 6816283]. 2. Bach, J., Eisenberg, F., Cantz, M. and Neufeld, E.C. The defect in the Hunter syndrome: deficiency of sulfoiduronate sulfatase. Proc. Natl. ...
MalaCards based summary : Chondrodysplasia Punctata, Tibia-Metacarpal Type, also known as chondrodysplasia punctata, tibial-metacarpal type, is related to chondrodysplasia punctata syndrome and otitis media. An important gene associated with Chondrodysplasia Punctata, Tibia-Metacarpal Type is ARSD (Arylsulfatase D). Affiliated tissues include bone, and related phenotypes are short 4th metacarpal and malar flattening ...
The present study indicates that de novo biosynthesis of progestogens and androgens from cholesterol is unlikely in human adipose tissue, because the mRNAs of key steroidogenic enzymes, such as cytochromes P450scc and P45c17, and of proteins specifically expressed in steroidogenic cells, such as StAR and SF-1, could not be demonstrated in any of the samples. The failure to amplify cytochrome P450c17 cDNA, even with two other sets of primers (data not shown), is at variance with the report by Puche and co-workers (2002), but can be explained by the fact that they used not only RT-PCR, but also Southern blotting analysis to intensify the signal. The level of expression must be minimal anyway.. Our data support an intracrine role of adipose tissue as a terminal activator of circulating inactive androgen precursors into potent sex steroids. The detection of P450arom mRNA confirms previous findings about the occurrence of aromatizing activity for estrogen synthesis in adipose tissue, as reviewed by ...
The new antitumor sulfamate EMD 486019 was investigated for its interaction with twelve catalytically active mammalian carbonic anhydrase (CA, EC 4.2.1.1) isozymes, hCA I - XIV. Similarly to 667-Coumate, a structurally related compound in phase II clinical trials as steroid sulfatase/CA inhibitor with potent antitumor properties, EMD 486019 acts as a strong inhibitor of isozymes CA II, VB, VII, IX, XII, and XIV (K(I)s in the range of 13-19nM) being less effective against other isozymes (K(I)s in the range of 66-3600nM against hCA I, IV, VA, VI, and mCA XIII, respectively). The complete inhibition profile of 667-Coumate against these mammalian CAs is also reported here for the first time. Comparing the X-ray crystal structures of the two adducts of CA II with EMD 486019 and 667-Coumate, distinct orientations of the bound sulfamates within the enzyme cavity were observed, which account for their distinct inhibition profiles. CA II/IX potent inhibitors belonging to the sulfamate class are thus ...
He, J., Gao, J., Xu, M., Ren, S., Stefanovic-Racic, M., ODoherty, R.M., Xie, W. PXR ablation alleviates diet-induced and genetic obesity and insulin resistance in mice. Diabetes. 2013; 62(6): 1876-87.. Shi, X., Cheng, Q., Xu, L., Yan, J., Jiang, M., He, J., Xu, M., Stefanovic-Racic, M., Sipula, I.J., ODoherty, R.M., Ren, S., Xie, W. Cholesterol sulfate and cholesterol sulfotransferase inhibit gluconeogenesis by targeting hepatocyte nuclear factor 4a. Molecular and Cellular Biology. 2014; 34(3): 485-97.. Jiang, M., He, J., Kucera, H., Gaikwad, N.W., Zhang, B., Xu, M., ODoherty, R.M., Selcer, K.W., Xie, W. Hepatic overexpression of steroid sulfatase ameliorates mouse models of obesity and type 2 diabetes through sex-specific mechanisms. The Journal of Biological Chemistry. 2014; 289(12): 8086-97.. Avin, K.G., Coen, P.M., Huang, W., Stolz, D.B., Sowa, G.A., Dubé, J.J., Goodpaster, B.H., ODoherty, R.M., Ambrosio, F. Skeletal muscle as a regulator of the longevity protein, Klotho. Frontiers in ...
The Triton Snail is found at moderate to shallow depths, anywhere in the region of 4 - 22m. If there is a coral rich area they may be found slightly deeper. The snail moves around the reef and is an active predator. In regions where the seastars; crown-of-thorns and cushion star can be found, the Triton snail plays an important role in reef community. They have the ability to immobilize the seastar with an injection of paralytic salivary juices. After immobilisation, which can take hours the Triton snail bores through the seastars mesh-like skeleton and eats the soft tissue inside. The snail has very sturdy radular teeth which are capable of ripping open the outer protective coating of its prey. Food may also be swallowed whole if it is small enough for consumption, otherwise the Triton snail immobilizes its prey and feeds on the soft body parts. ...
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ARSB antibody [N3C3] (arylsulfatase B) for WB. Anti-ARSB pAb (GTX102829) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
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... encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008 ...
Metachromatic leukodystrophy. Metachromatic leukodystrophy (MLD) is an autosomal recessive inherited disorder in which the desulfation of 3-0-sulfogalactosyl-containing glycolipids by arylsulfatase A (ASA) is defective. The clinical onset and severity of MLD is variable. The late infantile form typically presents in the second year of life, the juvenile form presents between age 4 and puberty, and the adult form may present at any age after puberty. Gait disturbance and mental regression are the earliest signs. Depending on the variant, other symptoms include blindness, seizures, and behavioral disturbances. Diagnosis of MLD is complicated by the fact that significant reduction of ASA activity may not prove MLD and that its presence does not exclude it. Significant reduction of ASA activity is observed in individuals homozygous for the pseudodeficiency allele. Normal ASA activity is observed in MLD patients with a deficiency of saposin B. Residual activity can be detected in patients with late ...
Metachromatic leukodystrophy (MLD) is a lysosomal storage disorder caused by a deficiency of the arylsulfatase A (ARSA) enzyme, which leads to the accumulation of galactosyl sulfatide (cerebroside sulfate) in the white matter of the central nervous system and in the peripheral nervous system. Galactosyl sulfatide and, to a smaller extent, lactosyl sulfatide, also accumulate within the kidney, gallbladder, and other visceral organs and are excreted in excessive amounts in the urine.. The 3 clinical forms of MLD are late-infantile, juvenile, and adult, depending on age of onset. All result in progressive neurologic changes and leukodystrophy demonstrated on magnetic resonance imaging. Late-infantile MLD is the most common (50%-60% of cases) and usually presents between age 1 to 2 years with hypotonia, clumsiness, diminished reflexes, and slurred speech. Progressive neurodegeneration occurs and most patients die within 5 years of the diagnosis. Juvenile MLD (20%-30% of cases) is characterized by ...
Metachromatic leukodystrophy (MLD), an autosomal recessively inherited lysosomal storage disorder, causes a deficiency of arylsulfatase A. This results in accumulation of sulfated glycolipids (sulphatide) within lysosomes of myelin forming cells in the central and peripheral nervous system and to a lesser extent in lysosomes of cells comprising the liver, kidneys, and gallbladder. The disease is characterized by progressive demyelination with wide variability in clinical onset and severity. Depending upon the age at onset and disease progression, MLD may be classified as late infantile (6 months to 4 years), early juvenile (4 to 6 years), late juvenile (6 to 16 years), and adult (,16 years). In the late infantile and early juvenile forms, blindness, gait disturbances, loss of speech, loss of hearing, and quadriparesis are common signs. In older children and adults the disease may present with gait disturbances, mental regression, and behavioral abnormalities. Disease progression, also variable, ...
Cerebroside sulfatase: …called arylsulfatase A (ASA), or cerebroside sulfatase. Arylsulfatase A deficiency allows certain harmful sulfur-containing lipids, known as sulfosphingolipids (also called sulfatides), to accumulate in nerve tissues of the central nervous system instead of being broken down. Sulfatides can also accumulate in nerve tissue in organs, such as the kidneys and…
Chondrodysplasia punctata (CDP) is a heterogenous group of skeletal dysplasias characterized by aberrant bone mineralization, manifesting radiologically as epiphyseal stippling. Among this group, brachytelephalangic dysplasia, a benign form of CDP (CDPX1), is probably under-reported. It is an X-linked recessive disorder and is characterized by a flat nasal tip, short columella and maxillary hypoplasia, involvement of terminal phalanges, and stippled chondrodystrophy. This paper presents a clinical series of 13 patients with brachytelephalangic dysplasia. These patients enrolled during 2002-2006 were re-evaluated and their dysmorphic features were compiled in a predesigned proforma. Skeletal survey, karyotype, cardiac evaluation, and ophthalmic evaluation were planned for all the cases. Out of 13 patients, 10 were males and three were females. All patients had flat facies, a depressed nasal bridge, a hypoplastic nose, a short philtrum, notched alae nasi, brachydactyly, and hypoplastic terminal phalanges.
Presents results of a data collected over a 10-year period on 1,778 service intervals in 1,111 Arsi (Bos indicus) cows at the Ethiopian Ministry of Agriculture ranch to analyse conception rate at first service, interval between services in animals returning for insemination, the number of short cycles, length of first oestrous cycle and season of calving, and concluded that the low frequency of normal cycles is to a large extent due to heats which remained undetected by visual observation in Arsi cows under artificial insemination programmes ...
Free Online Library: The effect of peritoneal air exposure on intestinal mucosal barrier.(Research Article, Report) by Gastroenterology Research and Practice; Health, general Air Health aspects Colorectal diseases Environmental aspects Risk factors Gastrointestinal diseases Intestinal diseases Intestinal mucosa
Evo Show & Shine - My Nasional Ride (Proton Wira 1.6xli) becoming Evolution III GSR with HKS Kansai kit. - My ride not Import its nasional for me...as she made in Malaysia..but its DNA still from Lancer...bit the kits & modes all Import....only chasis are still Proton.....hehehehehe ...This my Ride spec Real
iduronate 2 sulfatase Antibody 17140-1-AP has been identified with ELISA. 17140-1-AP detected band in {{ptg:PositiveWB}} with {{ptg:WesternTiter}} dilution...
And what do they do in return? Now is discovered the skeleton in the flagellated cells cupboard-they cannot reproduce the colony. They are sterile, and must leave reproduction to the big lazy-seeming cells who are only lazy, however, because they must store up food-materials to start the new colony fairly on its way. The grow and grow, bulge inwards, and finally come to float free in the centre space, where they still grow, meanwhile dividing up into a number of cells. In the end, they become perfect miniature colonies, burst out of their parent and swim happily away.. Volvox is thus a real individual; of the two kinds of cells each has given up something the better to fulfil its own special duty. There is division of labour, and, from the point of view of the species, each kind is meaningless without the other.. The division of labour in Volvox is that usually first seen in compound individuals-between the reproductive functions on the one side and all the rest on the other. In other words, ...
16-hydroxydehydroepiandrosterone sulfate: RN given refers to cpd with unspecified sulfate ester linkage locant & (3 beta,16 beta)-isomer
We have family coming to visit in about...oh, NOW. Family who have never actually been to our house, live and in person, since we moved here almost five years ago. Which means I have spent the last five hours FRANTICALLY trying to clean and organize everything so it actually looks like weve lived here for five years. Instead of...five hours. Its your lucky day, unlabeled box of mystery that we never unpacked because clearly your contents were never missed! Youre finally getting unpacked moved to an out-of-the-way spot in the basement! They have a three-and-a-half year old little girl. So thats four children, all five years old and younger. Here. In the house that I am now questioning cleaning up in the first place. WELCOME TO HELL, NOW WITH 25% MORE PRINCESS CRAP. Even the dog got all fancied up for the occasion. By which I mean bathed. WAT DOIN? WATER MAKES IMPOSSIBLY TINY LEGS MOAR IMPOSSIBLY TINY I HAZ NO IDEA WAT JUST HAPPEND Anyway, I better go take out the last of the trash and re-check that
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Chondrodysplasia punctata: Chondrodysplasia punctata is a very rare, little-understood disorder in which spots of opaque calcifications are observed in the epiphyseal cartilage at birth. Many infants die within the first year; those who live may exhibit dwarfism, mental retardation, and congenital cataracts.
In contrast, two proteins that belong to the electron transfer chain in the thylakoidal membrane were repressed: plastocyanin (PC) and ferredoxin-NADP reductase (FNR). Because these two proteins act in the final stages of electron transfer during the light phase of photosynthesis and FNR catalyses the production of NADPH+H+ required for CO2 assimilation, it could be hypothesized that the first physiological symptoms of S limitation result in an alteration of the coupling between the light and dark phases of photosynthesis leading to a depletion of C assimilation by the limitation of NADPH+H+ availability. Indeed, sulphate restriction is known to affect C assimilation leading to a reduction in photosynthetic activity and a distortion of glycolytic flux, which can be assumed as a repercussion of amino acid accumulation, itself resulting from a reduction of S assimilation into cysteine [11, 38]. These changes to proteins associated with C metabolism observed in our study may lead to the ...
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Myc-DDK-tagged ORF clone of Homo sapiens sulfatase 1 (SULF1), transcript variant 1 as transfection-ready DNA - 10 µg - OriGene - cdna clones
This experiment can be run on multiple samples allowing the student to study the variation of water saturation as a function of pore geometry as well as capillary pressure. When used in conjunction with the ARS-200, the student cam measure the resistivity of the partially saturated samples, for an accurate determination of the cementation exponent, m, the saturation exponent, n, and the Archie constant, a, used in the classic log analysis equation; ...
Ante-Nicene Fathers, Vol. VIII: Fathers of the Third and Fourth Centuries: The Twelve Patriarchs, Excerpts and Epistles, The Clementina, Apocrypha, Decretals, Memoirs of Edessa and Syriac Documents, Remains of the First Ages, full text etext at sacred-texts.com
QSota Health Tips: Ichthyosis - a disease with a genetic predisposition, characterized by disorder of keratinization of the skin. There are 2 forms of ichthyosis - normal and X
어린선(魚鱗癬, ichthyosis)은 최소 28개의 아종이 있는 전신 유전성 피부 질환이다. 아종에 관계없이 모든 어린선 환자들은 마르고 딱딱하며 얇은 비늘 모양의 피부를 띤다.[1] 어린선은 물고기를 의미하는 그리스어ichthys에서 유래된 명칭으로 전신에 물고기 비늘같은 인설(scale)을 보이는 질환을 총칭하는 질환군이다. 유전적인 것과 후천적인 것이 있으며 유전적인 것 중 가장 흔한 것은 심상성 어린선이며 주로 사지와 체간의 신전면 표피가 과각질화되어 피부가 물고기 비늘 모양으로 보인다. 후천적 어린선의 피부 모양은 심상성 어린선과 유사하며 림프종을 비롯한 여러 암과 갑상선기능저하증, 유전분증, 홍반성루푸스나 피부근염과 같은 자가면역질환, 후천성면역결핍증, 약제 등과 연관되어 발생할 수 있다.[2] 증상의 경우 심상성 어린선과 같은 미미한 ...
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Multiple sulfatase deficiency (MSD) is a rare disorder characterized by impaired activity of all known sulfatases. The gene mutated in this disease is SUMF1, which encodes a protein involved in a post-translational modification at the catalytic site of all sulfatases that is necessary for their function. SUMF1 strongly enhances the activity of sulfatases when coexpressed with sulfatase in Cos-7 cells. We performed a mutational analysis of SUMF1 in 20 MSD patients of different ethnic origin. The clinical presentation of these patients was variable, ranging from severe neonatal forms to mild phenotypes showing mild neurological involvement. A total of 22 SUMF1 mutations were identified, including missense, nonsense, microdeletion, and splicing mutations. We expressed all missense mutations in culture to study their ability to enhance the activity of sulfatases. Of the predicted amino acid changes, 11 (p.R349W, p.R224W, p.L20F, p.A348P, p.S155P, p.C218Y, p.N259I, p.A279V, p.R349Q, p.C336R, p.A177P)
Looking for online definition of pseudoautosomal region in the Medical Dictionary? pseudoautosomal region explanation free. What is pseudoautosomal region? Meaning of pseudoautosomal region medical term. What does pseudoautosomal region mean?
E. coli Source ß-Glucuronidases
ß-Glucuronidase is a ~290 kDa tetrameric protein with an isoelectric point of 4.8.1 Unlike the enzyme preparations from mollusks that naturally contain ß-glucuronidase and sulfatase activities in almost equal amounts, the preparation of ß-glucuronidase from E. coli is essentially free of sulfatase activity. The enzyme from E. coli has a high rate of hydrolytic activity and it retains this activity during hydrolysis better than similar enzymes that are more sensitive to changes in the concentration of ß-glucuronide conjugates. The enzyme preparation from E. coli has been shown to be useful for determining the presence of androsterone, 17-hydroxycorticosteroids, and estriol in urine.2 The E. coli enzyme has also been shown to be more active against estrogen conjugates than other sources of the enzyme.3
Optimal pH: 6-7

References
1. Kim, D-H, et al., Biol. Pharm.
1) Hypertrophy of the horny layer of the epidermis. (2) Appearance of thickened, horny, verruca-like scales (for example, warts and calluses) over the entire body, concentrated in flexural areas. (3) A condition marked by thickening of the outer layer of the skin, which is made of keratin (a tough, protective protein). It can result from normal use (corns, calluses), chronic inflammation (eczema), or genetic disorders (X-linked ichthyosis, ichthyosis vulgaris). See also: Lymphedema Fibrosis Lymphedema Stages Complications of Lymphedema Lymphedema ...
Formylglycine-generating enzyme (FGE) catalyzes the oxidation of a specific cysteine residue in nascent sulfatase polypeptides to formylglycine (FGly). This FGly is part of the active site of all sulfatases and is required for their catalytic activity. Here we demonstrate that residues 34-68 constitute an N-terminal extension of the FGE catalytic core that is dispensable for in vitro enzymatic activity of FGE but is required for its in vivo activity in the endoplasmic reticulum (ER), i.e. for generation of FGly residues in nascent sulfatases. In addition, this extension is needed for the retention of FGE in the ER. Fusing a KDEL retention signal to the C terminus of FGE is sufficient to mediate retention of an N-terminally truncated FGE but not sufficient to restore its biological activity. Fusion of FGE residues 1-88 to secretory proteins resulted in ER retention of the fusion protein. Moreover, when fused to the paralog of FGE (pFGE), which itself lacks FGly-generating activity, the FGE ...
As the trace element copper is essential, but extremely toxic in high concentrations, intracellular copper concentrations are tightly controlled. Once in the cell, copper is distributed by metallochaperones, including the small cytoplasmic protein ATOX1. ATOX1 plays an important role in the transfer of copper to the copper export P-type ATPases ATP7A and ATP7B to facilitate
3,5-dihydroxybenzoic acid 99-10-5 NMR spectrum, 3,5-dihydroxybenzoic acid H-NMR spectral analysis, 3,5-dihydroxybenzoic acid C-NMR spectral analysis ect.
4-Bromo-3,5-dihydroxybenzoic acid 16534-12-6 NMR spectrum, 4-Bromo-3,5-dihydroxybenzoic acid H-NMR spectral analysis, 4-Bromo-3,5-dihydroxybenzoic acid C-NMR spectral analysis ect.
Some travel awards will be granted to students and postdocs attending the Fourth International Volvox Conference (Volvox 2017) in St. Louis. Applications are due July 1, 2017. If you are planning to attend the meeting as a student or postdoc, you really should apply.. The application procedure is not too onerous, basically fill out a form and email it in. The size and number of awards are a bit vague, but if years past are any indication, theyll cover a substantial portion of the costs:. ...
Study on Prevalence of Gastrointestinal Nematodes and Coccidian Parasites Affecting Cattle in West Arsi zone, Ormia Regional State, Ethiopia Abstract.
Information on chondrodysplasia punctata, a condition that affects the development in babies. Learn the causes, symptoms, diagnosis and treatment for this rare disorder from St. Louis Childrens Hospital.
The effects of cold air on cardiovascular and cerebrovascular diseases were investigated in an experimental study examining blood pressure and biochemical indicators. Zhangye, a city in Gansu Province, China, was selected as the experimental site. Health screening and blood tests were conducted, and finally, 30 cardiovascular disease patients and 40 healthy subjects were recruited. The experiment was performed during a cold event during 27-28 April 2013. Blood pressure, catecholamine, angiotensin II (ANG-II), cardiac troponin I (cTnI), muscle myoglobin (Mb) and endothefin-1 (ET-1) levels of the subjects were evaluated 1 day before, during the 2nd day of the cold exposure and 1 day after the cold air exposure. Our results suggest that cold air exposure increases blood pressure in cardiovascular disease patients and healthy subjects via the sympathetic nervous system (SNS) that is activated first and which augments ANG-II levels accelerating the release of the norepinephrine and stimulates the renin
The effects of cold air on cardiovascular and cerebrovascular diseases were investigated in an experimental study examining blood pressure and biochemical indicators. Zhangye, a city in Gansu Province, China, was selected as the experimental site. Health screening and blood tests were conducted, and finally, 30 cardiovascular disease patients and 40 healthy subjects were recruited. The experiment was performed during a cold event during 27-28 April 2013. Blood pressure, catecholamine, angiotensin II (ANG-II), cardiac troponin I (cTnI), muscle myoglobin (Mb) and endothefin-1 (ET-1) levels of the subjects were evaluated 1 day before, during the 2nd day of the cold exposure and 1 day after the cold air exposure. Our results suggest that cold air exposure increases blood pressure in cardiovascular disease patients and healthy subjects via the sympathetic nervous system (SNS) that is activated first and which augments ANG-II levels accelerating the release of the norepinephrine and stimulates the renin
The ability to site-specifically conjugate a protein to a payload of interest (e.g., a fluorophore, small molecule pharmacophore, oligonucleotide, or other protein) has found widespread application in basic research and drug development. For example, antibody-drug conjugates represent a class of biotherapeutics that couple the targeting specificity of an antibody with the chemotherapeutic potency of a small molecule drug. While first generation antibody-drug conjugates (ADCs) used random conjugation approaches, next-generation ADCs are employing site-specific conjugation. A facile way to generate site-specific protein conjugates is via the aldehyde tag technology, where a five amino acid consensus sequence (CXPXR) is genetically encoded into the protein of interest at the desired location. During protein expression, the Cys residue within this consensus sequence can be recognized by ectopically-expressed formylglycine generating enzyme (FGE), which converts the Cys to a formylglycine (fGly) residue. The
... sea urchin embryo arylsulfatase EC 3.1.6.1; green algae arylsulfatase EC 3.1.6.1, which plays a role in the mineralization of ... "Phylogenetic conservation of arylsulfatases. cDNA cloning and expression of human arylsulfatase B". J. Biol. Chem. 265 (6): ... Grossman AR, de Hostos EL, Schilling J (1989). "Structure and expression of the gene encoding the periplasmic arylsulfatase of ... arylsulfatase C (ASD) and E (ASE); steryl-sulfatase EC 3.1.6.2 (STS), a membrane bound enzyme which hydrolyzes 3-beta-hydroxy ...
Arylsulfatase A is activated by saposin B (Sap B), a non-enzymatic proteinaceous cofactor. When the arylsulfatase A enzyme ... MLD is directly caused by a deficiency of the enzyme arylsulfatase A (ARSA) and is characterized by enzyme activity in ... "Arylsulfatase A Deficiency: Metachromatic Leukodystrophy, ARSA Deficiency". GeneReviews, 2006 Kishimoto Y, Hiraiwa M, O'Brien ... Blomqvist, M.; Gieselmann, V.; Månsson, J. E. (2011). "Accumulation of lysosulfatide in the brain of arylsulfatase A-deficient ...
Arylsulfatase B Farooqui, A.A. (1976). "The desulphation of hexosamine sulphates by arylsulphatase B". Experientia. 32 (10): ... Gorham, S.D.; Cantz, M. (1978). "Arylsulphatase B, an exo-sulphatase for chondroitin 4-sulphate tetrasaccharide". Hoppe- ... N-acetylgalactosamine-4-sulfatase (EC 3.1.6.12, chondroitinsulfatase, chondroitinase, arylsulfatase B, acetylgalactosamine 4- ... arylsulfatase), and chondroitin 4-sulfatase". Biochim. Biophys. Acta. 612 (2): 373-383. doi:10.1016/0005-2744(80)90120-5. PMID ...
... produces arylsulfatase, biphenomycin A, cinerubin A and cinerubin B. David, L; Duteurtre, M; ... Ueki, T; Sawada, Y; Fukagawa, Y; Oki, T (June 1995). "Arylsulfatase from Streptomyces griseorubiginosus S980-14". Bioscience, ...
It is believed to be caused by a deficiency in arylsulfatase A. Arylsulfatase A is a lysosomal sulfatase that is able to ... Saposin B extracts sulfatide from the membrane, which makes it accessible to arylsulfatase A. Arylsulfatase A can then ... Here, arylsulfatase A hydrolyzes the sulfate group. However, in order for this reaction to be carried out, a sphingolipid ... a lysosomal storage disease and may be caused because of a defect in arylsulfatase A, leading to an inability to degrade ...
Low arylsulphatase A activity can occur in healthy individuals. This poses a challenge in genetic testing, making it difficult ... Barth ML, Ward C, Harris A, Saad A, Fensom A (1994). "Frequency of arylsulphatase A pseudodeficiency associated mutations in a ...
Arylsulfatase test Arylsulfatase enzyme is present in most mycobacteria. The rate by which arylsulfatase enzyme breaks down ... 3 day arylsulfatase test is used to identify potentially pathogenic rapid growers such as M. fortuitum and M. chelonae. Slow ... growing M. marinum and M. szulgai are positive in the 14-day arylsulfatase test. Catalase, semiquantitative activity Most ...
Further analysis has linked alkaline phosphates and aryl sulfatases to a larger superfamily. Some of the common genes found in ... Research has shown a relationship between members of the alkaline phosphatase family with aryl sulfatases. The similarities in ...
1989). "Cloning and expression of human arylsulfatase A". J. Biol. Chem. 264 (2): 1252-9. PMID 2562955. Fleischer B, Zambrano F ...
Shankaran R, Ameen M, Daniel WL, Davidson RG, Chang PL (June 1991). "Characterization of arylsulfatase C isozymes from human ... Steroid sulfatase (STS), or steryl-sulfatase, formerly known as arylsulfatase C, is a sulfatase enzyme involved in the ... Munroe DG, Chang PL (February 1987). "Tissue-specific expression of human arylsulfatase-C isozymes and steroid sulfatase". ... arylsulfatase C, isozyme S". Mueller JW, Gilligan LC, Idkowiak J, Arlt W, Foster PA (October 2015). "The Regulation of Steroid ...
Farooqui, Bachhawat B. K. (1973). "Enzymic desulfation of cerebroside-3'-sulfate by chicken brain arylsulfatase". J. Neurochem ... was caused by the absence of Arylsulfatase A, an enzyme responsible for the breaking down on sulfatides. This discovery ...
Positive for arylsulfatase activity (3 days) and Tween 80 hydrolysis. Produces a low level of heatstable catalase and is ...
Tobacman, Joanne K. (2003-06-01). "Does deficiency of arylsulfatase B have a role in cystic fibrosis?". Chest. 123 (6): 2130- ... arylsulphatase - which is necessary for Mycobacterium tuberculosis virulence. As M. tuberculosis would use its host's sources ...
"Characterization of a Recombinant Thermostable Arylsulfatase from Deep-Sea Bacterium Flammeovirga pacifica". Journal of ...
Mutations in this gene result in a deficiency of arylsulfatase E. Only 50-60% of cases have been shown to have mutations in ... The mutation that leads to a deficiency in arylsulfatase E. (ARSE) occurs in the coding region of the gene.Absence of stippling ... This condition is also known as arylsulfatase E deficiency, CDPX1, and X-linked recessive chondrodysplasia punctata 1. The ... April 2008). "Clinical and molecular analysis of arylsulfatase E in patients with brachytelephalangic chondrodysplasia punctata ...
... and Sulf2 are new members of a superfamily of arylsulfatases, being closely related to arylsulfatase A, B (ARSA; ARSB) ... However, this paradigm changed after the discovery of two extracellular 6-O-S glucosamine arylsulfatases, Sulf1 and Sulf2. ... "Amino acid residues forming the active site of arylsulfatase A. Role in catalytic activity and substrate binding". The Journal ...
"Terminal 22q deletion associated with a partial deficiency of arylsulphatase A". Journal of Medical Genetics. 29 (6): 432-3. ...
... is a form of mucopolysaccharidosis caused by a deficiency in arylsulfatase B (ARSB). It is named after Pierre Maroteaux (1926 ... functional characterization of pathogenic mutations and polymorphisms in the arylsulfatase B gene". Mol. Genet. Metab. 94 (3): ...
no tolerance to 5% NaCl, positive for Tween 80 hydrolysis and for 10-day-arylsulfatase. Negative for production of nicotinic ...
Key differentiating features are negative tests for arylsulfatase and pyrazinamidase and susceptibility to antimycobacterial ...
The hydrolysis of substituted phenyl sulphates by the arylsulphatase of Alcaligenes metacaligenes". Biochem. J. 64: 216-221. ...
... has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. ...
"Defective endoplasmic reticulum-resident membrane protein CLN6 affects lysosomal degradation of endocytosed arylsulfatase A". J ...
This organism was found to be positive in a nitrate reduction test and negative in the aryl sulfatase test that were performed ... The 484t strain was found to be negative for tellurite reduction as well as activity with aryl sulphatase. The optimal growth ...
Growth occurs at 37 to 50 °C. The type strain is positive for 10-d arylsulfatase and pyrazinamidase. Negative for 3-d ... arylsulfatase, urease, nitrate reductase, semi-quantitative catalase, heat-stable catalase, acid phosphatase, b-galactosidase ...
arylsulfatase B deficiency. *arytena. *arytenoepiglottic. *arytenoid. *arytenoid articular surface of lamina of cricoid ...
arylsulfatase D. Locus tag. CR201_G0050936. See related. Ensembl:ENSPPYG00000020093 Gene type. protein coding. RefSeq status. ... AslA; Arylsulfatase A or related enzyme [Inorganic ion transport and metabolism]. cl23718. Location:40 → 563. ALP_like; ... XM_024241537.1 → XP_024097305.1 arylsulfatase D isoform X2. Conserved Domains (1) summary. cl23718. Location:40 → 333. ALP_like ... XM_009234566.2 → XP_009232841.1 arylsulfatase D isoform X1. UniProtKB/TrEMBL. H2PUT6 Related. ENSPPYP00000022473 Conserved ...
Arylsulfatase E pronunciation, Arylsulfatase E translation, English dictionary definition of Arylsulfatase E. n. Chiefly ... redirected from Arylsulfatase E). Also found in: Thesaurus, Medical, Acronyms.. Related to Arylsulfatase E: Arylsulfatase D ... Arylsulfatase E - definition of Arylsulfatase E by The Free Dictionary https://www.thefreedictionary.com/Arylsulfatase+E ...
Types include: Arylsulfatase A (also known as "cerebroside-sulfatase") Arylsulfatase B (also known as "N-Acetylgalactosamine-4- ... Arylsulfatase (EC 3.1.6.1, sulfatase, nitrocatechol sulfatase, phenolsulfatase, phenylsulfatase, p-nitrophenyl sulfatase, ... The hydrolysis of substituted phenyl sulphates by the arylsulphatase of Alcaligenes metacaligenes". Biochem. J. 64 (2): 216-221 ... Sulfatase") Steroid sulfatase (formerly known as "Arylsulfatase C") ARSC2 ARSD ARSE ARSF ARSG ARSH ARSI ARSJ ARSK Aryl Dodgson ...
Arylsulfatase E, also known as ARSE, is an enzyme that, in humans, is encoded by the ARSE gene. Arylsulfatase E is a member of ... Urbitsch P, Salzer MJ, Hirschmann P, Vogt PH (2000). "Arylsulfatase D gene in Xp22.3 encodes two protein isoforms". DNA Cell ... 1998). "Biochemical characterization of arylsulfatase E and functional analysis of mutations found in patients with X-linked ... 1997). "Identification by shotgun sequencing, genomic organization, and functional analysis of a fourth arylsulfatase gene ( ...
DeLuca C, Brown JA, Shows TB (1979). "Lysosomal arylsulfatase deficiencies in humans: Chromosome assignments for arylsulfatase ... Arylsulfatase A is inhibited by phosphate, which forms a covalent bond with the active site 3-oxoalanine. GRCh38: Ensembl ... In humans, arylsulfatase A is encoded by the ARSA gene. A deficiency is associated with metachromatic leukodystrophy, an ... 1992). "Two new arylsulfatase A (ARSA) mutations in a juvenile metachromatic leukodystrophy (MLD) patient". Am. J. Hum. Genet. ...
Medical Dictionary, Arylsulfatase B function. Protein Data Base (PDB), Arylsulfatase structure. Genetics Home Reference, ... Arylsulfatase B is among a group of arylsulfatase enzymes present in the lysosomes of the liver, pancreas, and kidneys of ... An arylsulfatase B deficiency can lead to an accumulation of GAGs in lysosomes, which in turn can lead to mucopolysaccharidosis ... Decline in arylsulfatase B leads to increased invasiveness of melanoma cells. Oncotarget. December 1, 2016. Bhattacharyya S, ...
Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel ... ARYLSULFATASE A protein, length: 489 (BLAST) Sequence Similarity Cutoff. Rank. Chains in Cluster. Cluster ID / Name. Structural ...
This disorder is distinct from conditions caused by deficiencies of arylsulfatase B (Maroteaux-Lamy disease) and arylsulfatase ... Arylsulfatase A is also deficient in individuals with multiple sulfatase deficiency.. ... Individuals with pseudodeficiency of arylsulfatase A may have decreased enzyme activity and are not clinically affected with ...
Compare Arylsulfatase A ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and ... Arylsulfatase A ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and ... Your search returned 129 Arylsulfatase A ELISA ELISA Kit across 17 suppliers. ...
Arylsulfatase A, B, and C have been separated. A deficiency of arylsulfatases is one of the causes of metachromatic ... Arylsulfatases: Enzymes that catalyze the hydrolysis of a phenol sulfate to yield a phenol and sulfate. ... "Study of the molecular forms of arylsulfatases confirmed the complete deficiency of arylsulfatase A and arylsulfatase B ... Arylsulfatases. Subscribe to New Research on Arylsulfatases Enzymes that catalyze the hydrolysis of a phenol sulfate to yield a ...
1.3 A Structure of Arylsulfatase from Pseudomonas Aeruginosa Establishes the Catalytic Mechanism of Sulfate Ester Cleavage in ... ARYLSULFATASE protein, length: 536 (BLAST) Sequence Similarity Cutoff. Rank. Chains in Cluster. Cluster ID / Name. Structural ...
This does lead to low levels of arylsulfatase (ARSA).[PMID 1678251. ] Other mutations in the ARSA gene lead to metachromatic ... Although arylsulfatase A pseudodeficiency is characterized as a disease, about 1-2% of any population of clinically healthy ... Retrieved from "https://www.SNPedia.com/index.php?title=Arylsulfatase_A_pseudodeficiency&oldid=1059118" ...
Arylsulfatase BImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a ... tr,F6TYS7,F6TYS7_HORSE Arylsulfatase B OS=Equus caballus OX=9796 GN=ARSB PE=4 SV=2 ...
The conditions established are such that arylsulfatase A activity is minimal and its residual activity can be accounted for and ... subtracted from the activity of ARSB.(Baum H, Dodgson KS, Spencer B: Assay of arylsulfatases A and B in human urine. Clin Chim ... Arylsulfatase B (ARSB) can be measured with nitrocatechol sulfate as the substrate. ... Arylsulfatase B (ARSB) can be measured with nitrocatechol sulfate as the substrate. The conditions established are such that ...
... arylsulfatase activity, glycosphingolipid metabolic process, post-translational protein modification ... arylsulfatase activity Source: HGNC ,p>Traceable Author Statement,/p> ,p>Used for information from review articles where the ... R-HSA-1663150. The activation of arylsulfatases. Miscellaneous databases. ChiTaRS: a database of human, mouse and fruit fly ... sp,Q5FYB0,ARSJ_HUMAN Arylsulfatase J OS=Homo sapiens OX=9606 GN=ARSJ PE=2 SV=1 ...
Selected quality suppliers for anti-Arylsulfatase B antibodies. ... Order monoclonal and polyclonal Arylsulfatase B antibodies for ... arylsulfatase B structural , arylsulfatase B temporal regulation , Arylsulfatase B (MPS VI) , arylsulfatase B , arylsulfatase b ... Arylsulfatase B (ARSB) Polyclonal Host: Rabbit WB ELISA IgG Biotin FITC HRP IHC Reactivity: Cow (Bovine) ... Arylsulfatase B (ARSB) Antigen Profile Protein Summary Arylsulfatase B encoded by this gene belongs to the sulfatase family. ...
Pseudomonas aeruginosa arylsulfatase (PAS) is a bacterial sulfatase capable ofhydrolyzing a range of sulfate esters. Recently, ... Examining the promiscuous phosphatase activity of Pseudomonas aeruginosa arylsulfatase: A comparison to analogous phosphatases ...
... arylsulfatase D) for ELISA, ICC/IF, IHC-P. Anti-Arylsulfatase D pAb (GTX87652) is tested in Human samples. 100% Ab-Assurance. ... arylsulfatase D. Background. The protein encoded by this gene is a member of the sulfatase family. Sulfatases are essential for ... Storage Conditions: Arylsulfatase D antibody. Storage Buffer. Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM ... The antiserum was produced against synthesized peptide derived from human Arylsulfatase D. ...
Electrophoretic Characterization of A and B Isoenzymes of Arylsulfatase G. DUBOIS; G. DUBOIS ... G. DUBOIS, J. C. TURPIN, N. BAUMANN; Electrophoretic Characterization of A and B Isoenzymes of Arylsulfatase. Biochem Soc Trans ...
Arylsulfatase A (transcript variant 1). Not available. Recombinant protein of human arylsulfatase A (ARSA), transcript variant ... Arylsulfatase A (transcript variant 2). Not available. Purified recombinant protein of Homo sapiens arylsulfatase A (ARSA), ... Arylsulfatase A (transcript variant 4). Not available. Purified recombinant protein of Homo sapiens arylsulfatase A (ARSA), ... Arylsulfatase A (transcript variant 1). Not available. Recombinant protein of human arylsulfatase A (ARSA), transcript variant ...
OMIM: ARYLSULFATASE I; ARSI*Gene Ontology: Arsi *Mouse Phenome DB: Arsi *UCSC: Chr.18:60,912,240-60,917,768(+)*IMPC: Arsi ... arylsulfatase i. Gene nomenclature, locus information, and GO, OMIM, and PMID associations are updated daily from MGI ...
... arylsulfatase A) for WB. Anti-Arylsulfatase A pAb (GTX106155) is tested in Human samples. 100% Ab-Assurance. ... Storage Conditions: Arylsulfatase A antibody [N2C2], Internal. Storage Buffer. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). ... arylsulfatase A. Background. The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. ... Specifications: Arylsulfatase A antibody [N2C2], Internal. Full Name. ...
The pig endometrial arylsulphatase A was purified 3322-fold to a specific activity of 150 mumol/min per mg. The purification ... The pig endometrial arylsulphatase A shows many properties in common with those of arylsulphatases A purified from other ... Isolation and characterization of the pig endometrial arylsulphatase A. Message Subject (Your Name) has forwarded a page to you ... Isolation and characterization of the pig endometrial arylsulphatase A.. H Rahi, P N Srivastava ...
Electrophoretic Characterization of A and B Isoenzymes of Arylsulfatase. G. DUBOIS, J. C. TURPIN, N. BAUMANN ... Electrophoretic Characterization of A and B Isoenzymes of Arylsulfatase Message Subject (Your Name) has forwarded a page to you ...
  • M. abscessus has also been shown to produce arylsulfatase but not of nitrate reductase and Tween 80 hydrolase. (wikipedia.org)
  • Isolates do not grow on MacConkey agar or with 5% NaCl, are negative for arylsulfatase (14 days), b-galactosidase, nitrate reductase, pyrazinamidase (7 days), semiquantitative catalase, Tween 80 hydrolysis and Tween opacity and have variable reactions for acid phosphatase and catalase at 68 °C. The type strain is resistant to p-aminosalicylic acid and isoniazid but susceptible to ethambutol, ethionamide, kanamycin, rifampicin and streptomycin in disc susceptibility tests. (wikipedia.org)
  • Regulation of chondroitin-4-sulfotransferase (CHST11) expression by opposing effects of arylsulfatase B on BMP4 and Wnt9A. (nih.gov)
  • We propose that a contributing mechanism for the neurological damage is that lead induces critically low levels of arylsulfatase A (ASA) at sensitive stages of nervous system development. (researchwithrutgers.com)
  • Purification and biosynthesis of a derepressible periplasmic arylsulfatase from Chlamydomonas reinhardtii. (rupress.org)
  • Genome-wide association study in musician's dystonia: a risk variant at the arylsulfatase G locus? (cdc.gov)