Aryldialkylphosphatase: An enzyme which catalyzes the hydrolysis of an aryl-dialkyl phosphate to form dialkyl phosphate and an aryl alcohol. It can hydrolyze a broad spectrum of organophosphate substrates and a number of aromatic carboxylic acid esters. It may also mediate an enzymatic protection of LOW DENSITY LIPOPROTEINS against oxidative modification and the consequent series of events leading to ATHEROMA formation. The enzyme was previously regarded to be identical with Arylesterase (EC 3.1.1.2).Phosphoric Triester Hydrolases: A class of enzymes that catalyze the hydrolysis of one of the three ester bonds in a phosphotriester-containing compound.Parathion: A highly toxic cholinesterase inhibitor that is used as an acaricide and as an insecticide.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Flavobacterium: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in SOIL and WATER. Its organisms are also found in raw meats, MILK and other FOOD, hospital environments, and human clinical specimens. Some species are pathogenic in humans.Insecticides: Pesticides designed to control insects that are harmful to man. The insects may be directly harmful, as those acting as disease vectors, or indirectly harmful, as destroyers of crops, food products, or textile fabrics.Methyl Parathion: The methyl homolog of parathion. An effective, but highly toxic, organothiophosphate insecticide and cholinesterase inhibitor.Carboxylic Acids: Organic compounds containing the carboxy group (-COOH). This group of compounds includes amino acids and fatty acids. Carboxylic acids can be saturated, unsaturated, or aromatic.EsterasesParaoxon: An organophosphate cholinesterase inhibitor that is used as a pesticide.EstersOrganophosphate Poisoning: Poisoning due to exposure to ORGANOPHOSPHORUS COMPOUNDS, such as ORGANOPHOSPHATES; ORGANOTHIOPHOSPHATES; and ORGANOTHIOPHOSPHONATES.Flax: A plant genus of the family LINACEAE that is cultivated for its fiber (manufactured into linen cloth). It contains a trypsin inhibitor and the seed is the source of LINSEED OIL.Lignans: A class of dibenzylbutane derivatives which occurs in higher plants and in fluids (bile, serum, urine, etc.) in man and other animals. These compounds, which have a potential anti-cancer role, can be synthesized in vitro by human fecal flora. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Dietary Supplements: Products in capsule, tablet or liquid form that provide dietary ingredients, and that are intended to be taken by mouth to increase the intake of nutrients. Dietary supplements can include macronutrients, such as proteins, carbohydrates, and fats; and/or MICRONUTRIENTS, such as VITAMINS; MINERALS; and PHYTOCHEMICALS.Risk Factors: An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.Linolenic Acids: Eighteen-carbon essential fatty acids that contain three double bonds.Lipids: A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)Exercise Therapy: A regimen or plan of physical activities designed and prescribed for specific therapeutic goals. Its purpose is to restore normal musculoskeletal function or to reduce pain caused by diseases or injuries.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Diffusion: The tendency of a gas or solute to pass from a point of higher pressure or concentration to a point of lower pressure or concentration and to distribute itself throughout the available space. Diffusion, especially FACILITATED DIFFUSION, is a major mechanism of BIOLOGICAL TRANSPORT.Phthalic Acids: A group of compounds that has the general structure of a dicarboxylic acid-substituted benzene ring. The ortho-isomer is used in dye manufacture. (Dorland, 28th ed)Lecithin Acyltransferase Deficiency: An autosomal recessively inherited disorder caused by mutation of LECITHIN CHOLESTEROL ACYLTRANSFERASE that facilitates the esterification of lipoprotein cholesterol and subsequent removal from peripheral tissues to the liver. This defect results in low HDL-cholesterol level in blood and accumulation of free cholesterol in tissue leading to a triad of CORNEAL OPACITY, hemolytic anemia (ANEMIA, HEMOLYTIC), and PROTEINURIA.Phosphatidylcholine-Sterol O-Acyltransferase: An enzyme secreted from the liver into the plasma of many mammalian species. It catalyzes the esterification of the hydroxyl group of lipoprotein cholesterol by the transfer of a fatty acid from the C-2 position of lecithin. In familial lecithin:cholesterol acyltransferase deficiency disease, the absence of the enzyme results in an excess of unesterified cholesterol in plasma. EC 2.3.1.43.Hypolipoproteinemias: Conditions with abnormally low levels of LIPOPROTEINS in the blood. This may involve any of the lipoprotein subclasses, including ALPHA-LIPOPROTEINS (high-density lipoproteins); BETA-LIPOPROTEINS (low-density lipoproteins); and PREBETA-LIPOPROTEINS (very-low-density lipoproteins).Cholesterol Esters: Fatty acid esters of cholesterol which constitute about two-thirds of the cholesterol in the plasma. The accumulation of cholesterol esters in the arterial intima is a characteristic feature of atherosclerosis.Sterol O-Acyltransferase: An enzyme that catalyzes the formation of cholesterol esters by the direct transfer of the fatty acid group from a fatty acyl CoA derivative. This enzyme has been found in the adrenal gland, gonads, liver, intestinal mucosa, and aorta of many mammalian species. EC 2.3.1.26.Cholesterol: The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.PhenoxyacetatesEcthyma, Contagious: An infectious dermatitis of sheep and goats, affecting primarily the muzzle and lips. It is caused by a poxvirus and may be transmitted to man.Orf virus: The type species of PARAPOXVIRUS which causes a skin infection in natural hosts, usually young sheep. Humans may contract local skin lesions by contact. The virus apparently persists in soil.Polymorphism, Genetic: The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Coronary Artery Disease: Pathological processes of CORONARY ARTERIES that may derive from a congenital abnormality, atherosclerotic, or non-atherosclerotic cause.Polymorphism, Restriction Fragment Length: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.Dissertations, Academic as Topic: Dissertations embodying results of original research and especially substantiating a specific view, e.g., substantial papers written by candidates for an academic degree under the individual direction of a professor or papers written by undergraduates desirous of achieving honors or distinction.Genotype: The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.Polymorphism, Single Nucleotide: A single nucleotide variation in a genetic sequence that occurs at appreciable frequency in the population.Preventive Medicine: A medical specialty primarily concerned with prevention of disease (PRIMARY PREVENTION) and the promotion and preservation of health in the individual.Lactones: Cyclic esters of hydroxy carboxylic acids, containing a 1-oxacycloalkan-2-one structure. Large cyclic lactones of over a dozen atoms are MACROLIDES.Dermatoglyphics: The study of the patterns of ridges of the skin of the fingers, palms, toes, and soles.DNA Fingerprinting: A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.Research: Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)Diving: An activity in which the organism plunges into water. It includes scuba and bell diving. Diving as natural behavior of animals goes here, as well as diving in decompression experiments with humans or animals.Biomedical Research: Research that involves the application of the natural sciences, especially biology and physiology, to medicine.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.TexasCarboxylic Ester Hydrolases: Enzymes which catalyze the hydrolysis of carboxylic acid esters with the formation of an alcohol and a carboxylic acid anion.Geobacillus: A genus of gram-positive, endospore-forming, thermophilic bacteria in the family BACILLACEAE.4-Butyrolactone: One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.

Isolation and complete covalent structure of liver microsomal paraoxonase. (1/676)

Paraoxonase (PON1) is a serum esterase exclusively associated with high-density lipoproteins; it might confer protection against coronary artery disease by destroying pro-inflammatory oxidized lipids in oxidized low-density lipoproteins. Here I show that rabbit liver microsomes contain a PON analogue (MsPON) and report the isolation and complete covalent structure of MsPON. In detergent-solubilized microsomes, MsPON co-purifies with the microsomal triacylglycerol transfer protein (MTP) complex. MsPON was separated from the complex and purified to homogeneity under non-denaturing conditions. Automated sequence analysis of intact MsPON and peptides obtained from enzymic and chemical cleavages led to the elucidation of the complete covalent structure of MsPON. The protein is a single polypeptide consisting of 350 residues. The sequence of rabbit liver microsomal MsPON is 60% identical with that of rabbit serum PON1, and 84% identical with the sequence predicted by a human cDNA of unknown function, designated PON3. MsPON has a hydrophobic segment at the N-terminus that might serve to anchor the protein to the microsomal membrane or to the MTP complex. Unlike in the serum enzyme, two potential N-glycan acceptor sites in MsPON are not glycosylated. An absence of N-glycans was also indicated in the rabbit liver MTP. MsPON has a single free cysteine residue at position 38 and a disulphide bond between Cys-279 and Cys-348. The microsomal enzyme lacks three residues at the N-terminus that are present in the serum protein. MsPON lacks four residues at the C-terminus that are present in the rabbit serum protein but absent from human serum PON1. On the basis of the observation that MsPON displays a high degree of similarity with serum PON1, it is proposed that MsPON might have a function related to that of PON1 in serum high-density lipoprotein complexes.  (+info)

Lipoprotein-associated phospholipase A2, platelet-activating factor acetylhydrolase, generates two bioactive products during the oxidation of low-density lipoprotein: use of a novel inhibitor. (2/676)

A novel and potent azetidinone inhibitor of the lipoprotein-associated phospholipase A2 (Lp-PLA2), i.e. platelet-activating factor acetylhydrolase, is described for the first time. This inhibitor, SB-222657 (Ki=40+/-3 nM, kobs/[I]=6. 6x10(5) M-1.s-1), is inactive against paraoxonase, is a poor inhibitor of lecithin:cholesterol acyltransferase and has been used to investigate the role of Lp-PLA2 in the oxidative modification of lipoproteins. Although pretreatment with SB-222657 did not affect the kinetics of low-density lipoprotein (LDL) oxidation by Cu2+ or an azo free-radical generator as determined by assay of lipid hydroperoxides (LOOHs), conjugated dienes and thiobarbituric acid-reacting substances, in both cases it inhibited the elevation in lysophosphatidylcholine content. Moreover, the significantly increased monocyte chemoattractant activity found in a non-esterified fatty acid fraction from LDL oxidized by Cu2+ was also prevented by pretreatment with SB-222657, with an IC50 value of 5.0+/-0.4 nM. The less potent diastereoisomer of SB-222657, SB-223777 (Ki=6.3+/-0.5 microM, kobs/[I]=1.6x10(4) M-1.s-1), was found to be significantly less active in both assays. Thus, in addition to generating lysophosphatidylcholine, a known biologically active lipid, these results demonstrate that Lp-PLA2 is capable of generating oxidized non-esterified fatty acid moieties that are also bioactive. These findings are consistent with our proposal that Lp-PLA2 has a predominantly pro-inflammatory role in atherogenesis. Finally, similar studies have demonstrated that a different situation exists during the oxidation of high-density lipoprotein, with enzyme(s) other than Lp-PLA2 apparently being responsible for generating lysophosphatidylcholine.  (+info)

Paraoxonase 192 Gln/Arg gene polymorphism, coronary artery disease, and myocardial infarction in type 2 diabetes. (3/676)

Paraoxonase is an HDL-associated enzyme implicated in the pathogenesis of atherosclerosis by protecting lipoproteins against peroxidation. Its biallelic gene polymorphism at codon 192 (glutamine/arginine) has been associated with coronary artery disease (CAD). To further evaluate the role of this paraoxonase gene polymorphism for CAD in type 2 diabetes, we determined the paraoxonase genotype in 288 type 2 diabetic patients (170 with and 118 without angiographically documented CAD). The paraoxonase 192 Gln/Arg genotype was assessed using polymerase chain reaction followed by AlwI digestion. The frequency of the Gln allele was 0.656 in the CAD patients and 0.746 in the controls (chi2 = 5.36, P = 0.02). Compared with the Gln/Gln genotypes, the age-adjusted odds ratio for CAD was 1.78 (95% CI 1.08-2.96, P = 0.02) in subjects carrying at least one Arg allele. In the multivariate analysis, this association was even stronger after correction for the possible confounders age, sex, smoking history, and hypertension. Among current and former smokers, the odds ratio (OR) for having CAD among patients with at least one Arg allele was 3.58 (1.45-9.53, P < 0.01). The paraoxonase Arg allele was not associated with the history of myocardial infarction (OR 1.20 [0.73-1.99, NS]), but was with the extent of CAD (OR for three-vessel disease 1.92 [1.15-3.27, P = 0.01]). Our data indicate that the 192 Arg allele of the human paraoxonase gene is a risk factor for CAD but not myocardial infarction in type 2 diabetic patients, a risk factor further modified by cigarette smoking. This risk could possibly be explained by a reduced ability of the paraoxonase Arg isoform to protect lipoproteins against peroxidation.  (+info)

Role of group II secretory phospholipase A2 in atherosclerosis: 1. Increased atherogenesis and altered lipoproteins in transgenic mice expressing group IIa phospholipase A2. (4/676)

Some observations have suggested that the extracellular group IIa phospholipase A2 (sPLA2), previously implicated in chronic inflammatory conditions such as arthritis, may contribute to atherosclerosis. We have examined this hypothesis by studying transgenic mice expressing the human enzyme. Compared with nontransgenic littermates, the transgenic mice exhibited dramatically increased atherosclerotic lesions when maintained on a high-fat, high-cholesterol diet. Surprisingly, the transgenic mice also exhibited significant atherosclerotic lesions when maintained on a low-fat chow diet. Immunohistochemical staining indicated that sPLA2 was present in the atherosclerotic lesions of the transgenic mice. On both chow and atherogenic diets, the transgenic mice exhibited decreased levels of HDLs and slightly increased levels of LDLs compared with nontransgenic littermates. These data indicate that group IIa sPLA2 may promote atherogenesis, in part, through its effects on lipoprotein levels. These data also provide a possible mechanism for the observation that there is an increased incidence of coronary artery disease in many chronic inflammatory diseases.  (+info)

Role of group II secretory phospholipase A2 in atherosclerosis: 2. Potential involvement of biologically active oxidized phospholipids. (5/676)

Secretory nonpancreatic phospholipase A2 (group II sPLA2) is induced in inflammation and present in atherosclerotic lesions. In an accompanying publication we demonstrate that transgenic mice expressing group II sPLA2 developed severe atherosclerosis. The current study was undertaken to determine whether 1 mechanism by which group II sPLA2 might contribute to the progression of inflammation and atherosclerosis is by increasing the formation of biologically active oxidized phospholipids. In vivo measurements of bioactive lipids were performed, and in vitro studies tested the hypothesis that sPLA2 can increase the accumulation of bioactive phospholipids. We have shown previously that 3 oxidized phospholipids derived from the oxidation of 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (PAPC) stimulated endothelial cells to bind monocytes, a process that is known to be an important step in atherogenesis. We now show that these 3 biologically active phospholipids are significantly increased in livers of sPLA2 transgenic mice fed a high-fat diet as compared with nontransgenic littermates. We present in vitro evidence for several mechanisms by which these phospholipids may be increased in sPLA2 transgenics. These studies demonstrated that polyunsaturated free fatty acids, which are liberated by sPLA2, increased the formation of bioactive phospholipids in LDL, resulting in increased ability to stimulate monocyte-endothelial interactions. Moreover, sPLA2-treated LDL was oxidized by cocultures of human aortic endothelial cells and smooth muscle cells more efficiently than untreated LDL. Analysis by electrospray ionization-mass spectrometry revealed that the bioactive phospholipids, compared with unoxidized PAPC, were less susceptible to hydrolysis by human recombinant group II sPLA2. In addition, HDL from the transgenic mice and human HDL treated with recombinant sPLA2 in vitro failed, in the coculture system, to protect against the formation of biologically active phospholipids in LDL. This lack of protection may in part relate to the decreased levels of paraoxonase seen in the HDL isolated from the transgenic animals. Taken together, these studies show that levels of biologically active oxidized phospholipids are increased in sPLA2 transgenic mice; they also suggest that this increase may be mediated by effects of sPLA2 on both LDL and HDL.  (+info)

Reduced postprandial serum paraoxonase activity after a meal rich in used cooking fat. (6/676)

Paraoxonase is an enzyme associated with HDL in human serum that hydrolyzes oxidized phospholipids and inhibits LDL oxidation, which is an important step in atherogenesis. In animals, addition of oxidized lipids to the circulation reduces paraoxonase activity, and diets rich in oxidized fat accelerate the development of atherosclerosis. The current randomized, crossover study was designed to compare the effect of a meal rich in oxidized lipids in the form of fat that had been used for deep-frying in a fast food restaurant and a control meal rich in the corresponding unused fat on postprandial serum paraoxonase (arylesterase) activity and peroxide content of LDL and its susceptibility to copper ion catalyzed oxidation in 12 healthy men. Four hours into the postprandial period, serum paraoxonase activity had decreased significantly after the used fat meal (-17%, P=0.005) and had increased significantly after the meal rich in unused fat (14%, P=0. 005). These changes were significantly (P=0.003) different. A time-course study indicated that serum paraoxonase activity remained lower than baseline for up to 8 hours after the used fat meal. Serum apoA1 concentration tended to decrease after the unused fat meal and tended to increase after the used fat meal. These changes were different at a marginal level of significance (P=0.07). Also, a significantly (P=0.03) greater decrease in apoA1 content of postprandial HDL was recorded after the unused fat meal. The peroxide content of LDL tended to decrease after the used fat meal and tended to increase after the control meal. These changes were significantly (P=0.04) different. Susceptibility of isolated LDL to copper ion oxidation and plasma levels of malondialdehyde were unchanged during the study. These data suggest that in the postprandial period after a meal rich in used cooking fat, the enzymatic protection of LDL against accumulation of peroxides and atherogenic oxidative modification may be reduced, possibly due to factors associated with apoA1, without acutely affecting the intrinsic resistance of LDL to in vitro oxidation.  (+info)

Gln --> Arg 191 polymorphism of paraoxonase and Parkinson's disease. (7/676)

We investigated the Gln --> Arg 191 polymorphism in paraoxonase (PON1) in St. Petersburg population, in three clinically differentiated groups of patients with Parkinson's disease (PD) and in the symptomatic tremor group. A new approach for Gln --> Arg 191 PON1 polymorphism genotyping is suggested. No significant differences in the groups studies as compared to the controls was observed.  (+info)

Targeted disruption of the murine lecithin:cholesterol acyltransferase gene is associated with reductions in plasma paraoxonase and platelet-activating factor acetylhydrolase activities but not in apolipoprotein J concentration. (8/676)

Lecithin:cholesteryl acyltransferase (LCAT) deficiency resulting from targeted disruption of the Lcat gene in the mouse is associated with dramatic decreases in HDL concentration and the accumulation of nascent HDL in the plasma. We examined whether LCAT deficiency in mice is associated with a concomitant decrease in two antioxidative enzymes, paraoxonase (PON) and platelet-activating factor acetylhydrolase (PAF-AH). In control Lcat (+/+) mice both these enzymes are transported on HDL. Compared to Lcat (+/+) mice, HDL-cholesterol is reduced 94% and apoA-I, 90%, in Lcat (-/-) mice; this reduction in HDL is paralleled by a 71% decrease in PAF-AH activity and in a 58% decrease in PON activity. Apolipoprotein J (apoJ) levels, rather than being decreased, were significantly (P = 0.01) higher (36%) in Lcat (-/-) than in Lcat (+/+) mice, and the apo J/PON ratio was 3-fold greater in Lcat (-/-) than in Lcat (+/+) animals. Even though apolipoprotein A-I (apoA-I) concentration and PON activity were drastically reduced, there was no reduction in apoA-I and PON liver mRNA levels suggesting that post-transcriptional events are responsible for the reduction of plasma PON and apoA-I levels. Fast protein liquid chromatography (FPLC) revealed that in Lcat (+/+) mice both PON and PAF-AH activity is associated with large, apoA-I-containing HDL particles (9.7 nm by non-denaturing gradient gel electrophoresis) while in Lcat (-/-) mice both enzymes are associated with small 8.2 nm particles. We conclude that the concomitant reduction in HDL and apoA-I concentrations and PON and PAF-AH activities is best explained by rapid clearance of the small HDL particles found in LCAT deficiency.  (+info)

*Aryldialkylphosphatase

... (EC 3.1.8.1) (more commonly known as phosphotriesterase, and also organophosphate hydrolase, parathion ...

*Paraoxonase

... s are a family of mammalian enzymes with aryldialkylphosphatase activity. There are three paraoxonase isozymes, ...

*Phenols

Aryldialkylphosphatase (also known as organophosphorus hydrolase, phosphotriesterase, and paraoxon hydrolase) uses an aryl ...

*Aromatic alcohol

Aryldialkylphosphatase (also known as organophosphorus hydrolase, phosphotriesterase, and paraoxon hydrolase) uses an aryl ...

*PON1

Serum paraoxonase/arylesterase 1 (PON1) also known as A esterase , homocysteine thiolactonase or serum aryldialkylphosphatase 1 ...

*List of MeSH codes (D08)

... aryldialkylphosphatase MeSH D08.811.277.352.700 --- ribonucleases MeSH D08.811.277.352.700.350 --- endoribonucleases MeSH ...

*List of EC numbers (EC 3)

... aryldialkylphosphatase EC 3.1.8.2: diisopropyl-fluorophosphatase EC 3.1.11.1: exodeoxyribonuclease I EC 3.1.11.2: ...
TY - JOUR. T1 - A common mutation in paraoxonase-2 results in impaired lactonase activity. AU - Stoltz, David A.. AU - Ozer, Egon A.. AU - Recker, Thomas J.. AU - Estin, Miriam. AU - Yang, Xia. AU - Shih, Diana M.. AU - Lusis, Aldons J.. AU - Zabner, Joseph. PY - 2009/12/18. Y1 - 2009/12/18. N2 - Paraoxonases (PONs) are a family of lactonases with promiscuous enzyme activity that has been implicated in multiple diseases. PON2 is intracellularly located, is the most ubiquitously expressed PON, and has the highest lactonase activity of the PON family members. Whereas some single-nucleotide polymorphisms (SNPs) in PON1 have resulted in altered enzymatic activity in serum, to date the functional consequences of SNPs on PON2 function remain unknown. We hypothesized that a common PON2 SNP would result in impaired lactonase activity. Substitution of cysteine for serine at codon 311 in recombinant PON2 resulted in normal protein production and localization but altered glycosylation and decreased ...
Serum paraoxonase/arylesterase 1 (PON1) also known as A esterase , homocysteine thiolactonase or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. Paraoxonase 1 has esterase and more specifically paraoxonase activity. Serum PON1 is found in all mammalian species studied so far but is not present in the serum of birds, fish and reptiles or in insects. PON1 is the first discovered member of a multigene family also containing PON2 and PON3, the genes for which are located adjacent to each other on chromosome 7. Human PON1 is a glycoprotein composed of 354 amino acids and has a molecular weight of 43000 Daltons which associates with high-density lipoprotein (HDL, "good cholesterol") in the circulation. Serum PON1 is secreted mainly by the liver although local synthesis occurs in several tissues and PON1 protein is found in almost all tissues. X-ray crystallography has revealed the structure of PON1 to be a 6 bladed propeller with a unique lid structure covering ...
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Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.
Sigma-Aldrich offers abstracts and full-text articles by [M Aviram, M Rosenblat, S Billecke, J Erogul, R Sorenson, C L Bisgaier, R S Newton, B La Du].
Human serum paraoxonase/arylesterase 1 (PON1) standard, for use in running standard curves in AlphaLISA detection and quantitation assays
Background: High-density lipoprotein (HDL) cholesterol is well-established as a negative risk factor for coronary artery disease. Its antioxidant properties are attributed mainly to the HDL-bound enzyme, paraoxonase-1 (PON-1). Recently, myeloperoxidase (MPO) has been shown to attenuate the atheroprotective function of HDL. This study investigated the relationship between plasma MPO and serum PON-1 levels in patients with stable angina pectoris (SAP), and whether PON-1 levels predict cardiovascular events in SAP patients after stent implantation.. Methods: Plasma MPO levels and serum PON-1 concentration and activity were measured in patients with SAP (n=226) and in control subjects (n=96). In addition, we assessed the prognostic significance of serum PON-1 concentrations on admission after stent implantation in 263 SAP patients. Cardiac events were defined as sudden cardiac death, fatal or non-fatal myocardial infarction and other non-fatal events including unstable angina pectoris or coronary ...
Paraoxonases are a family of mammalian enzymes with aryldialkylphosphatase activity. There are three paraoxonase isozymes, which were originally discovered for their involvement in the hydrolysis of organophosphates. Research has indicated the enzymatic activity of paraoxonases is more diversified than its activity as an organophosphatase. Esterase and lactonase activity has also been observed from these enzymes and though the physiologically relevant substrates for these enzymes are unknown, it is likely that lactones are the main substrate (although there is a relatively high level of variation in substrate specificity among these enzymes). Most of the studies on the paraoxonase family have specifically looked at the paraoxonase 1 type, leaving much to be learned about the remaining two. The study of this enzyme family has many potential consequences in preventative medicine and toxicology as well as in certain societal contexts. The genes that encode for these enzymes have a number of ...
TY - JOUR. T1 - Association between the paraoxonase-1 192Q,R allelic variant and coronary endothelial dysfunction in patients with early coronary artery disease. AU - Lavi, Shahar. AU - McConnell, Joseph P.. AU - Lavi, Ronit. AU - Barsness, Gregory W.. AU - Rihal, Charanjit S.. AU - Novak, Gregory D.. AU - Lerman, Lilach O. AU - Lerman, Amir. PY - 2008. Y1 - 2008. N2 - OBJECTIVE: To test the hypothesis that allelic variants of the paraoxonase-1 gene are associated with endothelial dysfunction, an early stage of atherosclerosis. PATIENTS AND METHODS: We assessed 192Q,R and 55L,M allelic variants of the paraoxonase gene and coronary endothelial function in response to intracoronary acetylcholine in 99 patients (52 with homozygous QQ, 47 with homozygous RR or heterozygous QR). The study was conducted from September 1, 2002, through November 30, 2004. RESULTS: Of 52 homozygous QQ patients, 39 (75%) had endothelial dysfunction vs 20 (43%) of the 47 RR/QR patients (P=.001), and this association ...
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In a double blind clinical randomised clinical trial with parallel design this study was done.52 postmenopausal women were randomly assigned to 50 g/d soy protein or placebo for 10 weeks.serum lipoproteins and pon1 activity were measured at baseline and 10th week.There were significant increase in PON! activity and significant decrease in LDL-C,LDL-C/HDL-C,TC/HDL-C and TG/HDL-C in soy group compare to placebo group ...
Three paraoxonase genes (PON 1, PON 2, PON 3) have been mapped to chromosome 7 and a number of polymorphisms have been identified.32 PON prevents lipid peroxidation of LDLs and PON 1 has been shown to hydrolyze lipid peroxides in human atherosclerotic lesions.32 Oxidative modification of LDL cholesterol renders it particularly atherogenic, making identification of factors protecting against oxidative modification potentially important in the prevention of atherosclerosis.117 Two frequent PON 1 polymorphisms at positions 192 (glutamine to arginine) and 55 (methionine to leucine) are in linkage disequilibrium and are associated with variations in PON activity to different substrates.117 Associations between PON 1 and PON 2 polymorphisms and a variety of CVD phenotypes, however, have been inconsistent,32 with some studies showing the 55L allele and/or the 192R allele to be associated with increased risk118 and others showing no association.32 Both cigarette smoking and diabetes are associated with ...
The molecular bases underlying the alterations in the circulating PON1 activity together with its synthesis and secretion in chronic liver diseases are far from being completely understood, despite these changes being of crucial importance in the pathophysiology of chronic liver impairment. As illustrated in the present investigation, patients with liver cirrhosis had an increased serum concentration of total peroxides (a marker of oxidative stress), MCP-1 (an index of inflammation), and P-III-P (a marker of liver fibrogenesis). All these changes were observed to be strongly associated with a decrease in serum PON1 activity. Although a direct functional relationship between serum PON1 activity alterations, inflammation and fibrogenesis cannot be deduced from the present investigations, these data provide further support for studies showing that oxidative stress activates hepatic stellate cell and macrophages in vitro and in experimental models of liver impairment [32, 36-38]. A possible answer ...
Inbred mouse strains B6 and C3H have been studied extensively as a model of the genetic control of atherogenesis. In response to an atherogenic diet, B6 mice develop fatty streak lesions in the proximal aorta, whereas C3H mice are totally resistant to fatty streak formation. The present study was undertaken to determine the levels (plasma lipids, monocytes, and arterial wall cells) at which genetic variations affect atherogenesis in these 2 mouse strains.. In wild-type mouse models, atherosclerosis is induced by feeding a high-fat, high-cholesterol diet that contains cholate. In response to this diet, strain B6 mice have reduced levels of HDL compared with C3H mice.15 Moreover, this diet results in a reduction in serum paraoxonase, an HDL-associated enzyme that protects against LDL oxidation, in B6 mice but not in C3H mice.16 The apoE−/− mice represent a mouse model in which spontaneous hyperlipidemia and atherosclerosis occur with a low-fat, low-cholesterol diet.24 25 In the present study, ...
Genetic variants in the paraoxonase (PON) gene cluster, particularly a single C/T promoter polymorphism (rs 705381) in the PON-1 gene, have recently been associated with Alzheimers disease (AD). The T allele, in particular, presents an increased risk for the development of AD. Here, we investigate the potential role of this polymorphism in an Italian case-control population consisting of 306 sporadic AD patients and 275 controls, and also evaluate a possible interaction with the ApoE genotype. No association between the PON-1 polymorphism and AD was observed. The T allele frequency was slightly over-represented in AD patients compared to the controls, but this was far from being statistically significant. Our sample was evaluated to have 97.3% power to detect an OR of 2.0 (64.3% power with OR=1.5) at an alpha level of 0.05. No evidence of an interaction between the T risk-allele and the ApoE epsilon4 allele status and no effect of the PON-1 polymorphism on age at onset was detected. Our results ...
Mouse monoclonal antibody raised against full length recombinant PON1. Recombinant protein corresponding to full length human PON1. (MAB8230) - Products - Abnova
The purpose of this study is to investigate the critical role between promoter polymorphism of PON1 gene and its paraoxonase activity .....
The present study has demonstrated that glycation of apoA-I was associated with decreased activities of serum and HDL-associated PON1 and PON3. Elevated apoA-I glycation and reduced HDL-associated PON1 and PON3 activities, and the interaction of these two elements were related to the presence and severity of CAD in patients with T2DM.. HDL is an organized complex of proteins (apo and enzyme) and lipids (cholesterol, cholesteryl ester, triglyceride, and phospholipid), and possesses several functions with potential to protect against coronary atherosclerosis, by promoting efflux of cholesterol from macrophages in the arterial wall, inhibiting oxidative modification of low density lipoprotein, decreasing vascular inflammation, enhancing endothelial repair, and improving diabetic control [1, 2]. The structural and functional integrity of apoA-I is crucial for the activation and stability of lecithin:cholesterol acyl transferase (LCAT) and PON [10, 11]. Mutation, glycation and oxidative modification ...
Innovative Researchs Innovative Grade US Origin CD-1 Mouse Liver was recently used in the following study: Differences in amino acid residues in the binding pockets dictate substrate specificities of mouse senescence marker protein-30, human paraoxonase1, and squid ...T Belinskaya, N Pattabiraman, R diTargiani... - ... et Biophysica Acta (BBA ..., 2012 - Elsevier...2. Materials and methods. 2.1. Materials. Livers from CD-1 mice were purchased from Innovative Research Inc. (Novi, MI). All chromatography media and columns were purchased from GE Healthcare (Piscataway, NJ). Phenyl.... ...
We agree with the findings of Viktorinova and Kinova (1) in a recent pilot study of 75 nondiabetic white subjects (mean age 42.6 ± 7 years) with no prior history of cardiovascular disease and/or hypertension that examined the relationship between paraoxonase (PON1) enzyme activity and lipid risk factors. The authors found no significant differences in levels of HDL cholesterol and apolipoprotein (apo) A1 (apoA1) among normocholesterolemic (NC) and hypercholesterolemic (HC) men and women. However, serum PON1 activity was lower in HC groups (P , 0.05) compared with NC groups. In addition, they found positive correlations between PON1 activity and HDL cholesterol (r = 0.455, P , 0.05) and apoA1 (r = 0.697, P , 0.05) in NC women but not HC women or men. The authors postulated that low serum activity of PON1 and altered associations between PON1 activity and HDL as well as apoA1 may deteriorate beneficial functions of HDL and therefore a possible higher risk of initiation and progression of ...
The present study systematically investigated the constitutive and chemical regulation of mouse Pons. Consistent with previous reports, Pon1 and Pon3 are highly expressed in mouse liver, and Pon2 and Pon3 are expressed in the gastrointestinal tract (Mackness and Walker, 1983, 1988; Draganov et al., 2000; Ng et al., 2001). In addition, the present study indicates that Pon1, Pon2, and Pon3 all are expressed in mouse lung (Fig. 1), indicating paraxonases may play detoxification functions or other physiological roles in the lung.. In mice and rats, serum and liver Pon1 enzyme activity is very low at birth and increases until 21 days of age, with a parallel increase in liver mRNA (Li et al., 1997; Moser et al., 1998). In humans, serum PON1 activity is minimal before and at birth, but gradually increases after birth, reaching a plateau between 6 and 15 months of age (Augustinsson and Barr, 1963; Ecobichon and Stephens, 1973; Mueller et al., 1983; Cole et al., 2003). In the present study in mice, Pon1 ...
Abstract: Heart disease and stroke cause significant death and disability in industrialized nations. Oxidized low-density lipoprotein (ox-LDL) is an essential component of the plaque formation within arteries that leads to these conditions. Paraoxonase 1 (PON1) is an antioxidant enzyme capable of preventing the oxidation of LDL. The purpose of this study was to measure the relationship between ox-LDL concentration and PON1 activity in response to acute exercise. Fifteen aerobically trained individuals (M=12, F=3, VO2 max= 54.8 ± 1.7 mlkg-1min-1, BMI = 22.9± 0.5kgm-2) completed separate exercise treatment sessions at 60% and 80% VO2 max for 30 minutes. Ox-LDL concentration and PON1 activity were measured immediately before (PRE), immediately post (0 POST), and 15 minutes post (15 POST) exercise. To ascertain the degree of oxidative stress induced by the exercise sessions, protein carbonyl (PC) concentration and total antioxidant capacity (TAC) were measured at PRE and 0 POST. Repeated ...
Any time DJ Pon-3 is mixing records for a party, the tickets are sure to sell out. This stylish DJ knows a thing or two about beat matching, and she takes prid
Recombinant Paraoxonase 1 (PON1) Protein (GST tag). Species: Human. Source: Escherichia coli (E. coli). Order product ABIN4975995.
Mouse polyclonal antibody raised against a full-length recombinant PON2. PON2 (AAH46160, 1 a.a. ~ 219 a.a) full-length recombinant protein with GST tag. (H00005445-A01) - Products - Abnova
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
چکیده: زمینه و هدف: پاراکسوناز-1، آدیپونکتین و پراکسید هیدروژن از جمله شاخص هایی هستند که تحت تأثیر کم تحرکی قرار می گیرند و این احتمال وجود دارد که بر فشار خون موثر باشند. با این حال ارتباط بین تغییرات آن ها با فشار خون در اثر شرکت در فعالیت های منظم ورزشی مشخص نشده است، لذا هدف از تحقیق حاضر بررسی اثر 12 هفته فعالیت هوازی شدت متوسط بر ارتباط بین تغییرات پاراکسوناز-1، پراکسید هیدروژن و آدیپونکتین پلاسمایی با فشار خون سیستولی و دیاستولی مردان دارای فشار خون بالا می باشد. روش بررسی: تحقیق حاضر از نوع نیمه تجربی با اندازه گیری مکرر می باشد که در آن 10 مرد (در ...
PON3兔单克隆抗体[EPR2903(2)](ab109258)可与小鼠, 大鼠, 人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
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(Intro) Mi nah tell nuh lie the party so nice The amounta a gyal deh by mi side Mi have gyal pon the lef an pon the right Mi sure mi nah go home tonight
У кого 8 портовик? Пожалуйста подскажите сколько по питанию кушает (Вт?)
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Background: High-density lipoprotein (HDL) cholesterol is well-established as a negative risk factor for coronary artery disease (CAD) and its anti-oxidant property has been attributed mainly to the HDL-bound enzyme paraoxonase-1 (PON-1). Recently, myeloperoxidase (MPO), a pro-oxidant enzyme released from activated neutrophils, has been shown to reverse the atheroprotective action of HDL. The aim of this study was to investigate the relationship between plasma MPO and serum PON-1 levels in patients with stable (SAP) and unstable angina pectoris (UAP).. Methods: Plasma MPO and serum PON-1 concentrations and activity were measured in patients with SAP (n=230) and UAP (n=151), and in control subjects (n=102).. Results: Plasma MPO levels were significantly higher in UAP patients than in SAP patients or control subjects (UAP, 21.6 [16.7-44.6]; SAP, 19.2 [15.6-29.1]; control, 16.0 [14.8-18.9] ng/ml; P,0.0001). Furthermore, serum PON-1 concentrations were significantly lower in UAP and SAP patients ...
Primary Objective:. Atherosclerosis of the carotid arteries is a common cause of stroke. The prevalence and progression of carotid atherosclerosis are believed to be influenced by genetically inherited variations in lipoprotein metabolism. This study investigates the specific role of paraoxonase, an enzyme thought to detoxify atherogenic oxidized low-density lipoprotein. This study compares veterans who have significant carotid atherosclerosis on ultrasound examination with controls without carotid atherosclerosis. Both paraoxonase activity and genotype will be determined and compared between groups. The results may eventually make it possible to screen for a paraoxonase allele that confers high risk of atherosclerosis, and to diminish the risk by early treatment.. Study Abstract:. The general aim of the proposed research is to evaluate the contribution and mechanism of paraoxonase (PON1) genotypic and phenotypic variation (PON1 status) in risk and progression of carotid artery disease (CAAD). ...
Paraoxonase-1 (PON1) is an antioxidant enzyme, that resides on high-density lipoprotein (HDL). PON1-activity, is heavily influenced by the PON1-Q192R polymorphism. PON1 is considered to protect against atherosclerosis, but it is unclear whether this relation is independent of its carrier, HDL. In order to evaluate the atheroprotective potential of PON1, we assessed the relationships among PON1-genotype, PON1-activity and risk of future coronary artery disease (CAD), in a large prospective case-control study. Methodology/Principal Findings: Cases (n = 1138) were apparently healthy men and women aged 45-79 years who developed fatal or nonfatal CAD during a mean follow-up of 6 years. Controls (n = 2237) were matched by age, sex and enrollment time. PON1-activity was similar in cases and controls (60.7 +/- 645.3 versus 62.6 +/- 645.8 U/L, p = 0.3) and correlated with HDL-cholesterol levels (r = 0.16, p , 0.0001). The PON1-Q192R polymorphism had a profound impact on PON1-activity, but did not predict ...
Human plasma paraoxonase (PON1) has been shown to have arylesterase and paraoxonase activity. This high-density lipoprotein (HDL) associated enzyme exhibits antiatherogenic properties and acts as a detoxifying agent for several chemical warfare agents and insecticides. We show that the reported purification process (Gan et al.) contains a ~68kDa co-purifying contaminant. We have developed a modified procedure using size exclusion chromatography to obtain pure PON1 from human serum. In order to support the current homology model of PON1 developed using the crystal structure of DFPase as a model, a CD spectrum of pure, monodisperse PON1 was measured. Previous attempts were inconclusive due to a high background caused by detergent micelle light scattering. The detergent free form of PON1 has been characterized to exist as monomer, dimer, and higher order soluble aggregates. Thus, detergents are necessary to retain native, monodisperse enzyme. In conjunction with our modified purification procedure, ...
PON2 - PON2 (Myc-DDK-tagged)-Human paraoxonase 2 (PON2), transcript variant 2 available for purchase from OriGene - Your Gene Company.
International Journal of Hepatology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies related to the medical, surgical, pathological, biochemical, and physiological aspects of hepatology, as well as the management of disorders affecting the liver, gallbladder, biliary tree, and pancreas.
The link between HDL and cardiovascular disease (CVD) is substantially more complex than ) originally thought. Until recently, the concentration of HDL was deemed sufficient in predicting future cardiovascular events. However, HDL function can become compromised in various disease states, resulting in reduced cardio-protection and the production of dysfunctional HDL with reduced anti-atherogenic properties. Antioxidants have been implicated in ameliorating the damaging oxidative processes associated with dysfunctional HDL and atherosclerosis. Therefore, this thesis focuses on the evolving role of HDL function and had three main aims: i) to assess the complex relationship between HDL-cholesterol concentration and HDL function, ii) to assess the modulation of HDL function by the ) predominant form of vitamin E, α-tocopherol, encompassing in vitro and ex vivo investigations and iii) to assess the modulation of HDL function by lycopene by dietary interventions. Various HDL-associated enzymes, ...
Definition : Immunoassay reagents intended to perform quantitative analyses of body fluids (typically plasma or serum) to determine the level of the lipoprotein-associated enzyme phospholipase A2 (Lp-PLA2). These tests are usually based on turbidimetric or ELISA tests that use specific antibodies to identify the level of (Lp-PLA2). They may be used as an aid to assess vascular inflammation and the risk for coronary heart disease and ischemic stroke associated with atherosclerosis.. Entry Terms : "Lipoprotein-Associated Phospholipase A2 Determination Reagents" , "Lp-PLA2 Determination Reagents" , "PLAC Test ELISA Kits" , "PLAC Test Reagent Kits". UMDC code : 32900 ...
Lovrić, Jasna and Berend, Suzana and Lucić Vrdoljak, Ana and Radić, Božica and Katalinić, Maja and Kovarik, Zrinka and Želježić, Davor and Kopjar, Nevenka and Rast, Slavko and Mesić, Milan (2011) A conjugate of pyridine-4-aldoxime and atropine as a potential antidote against organophosphorus compounds poisoning. Acta Biochimica Polonica, 58 (2). pp. 193-8. ISSN 0001-527X Macan, Marija and Vrkić, Nada and Lucić Vrdoljak, Ana and Radić, Božica and Bradamante, Vlasta (2010) Effects of high sucrose diet, gemfibrozil, and their combination on plasma paraoxonase 1 activity and lipid levels in rats. Acta Biochimica Polonica, 57 (3). pp. 321-6. ISSN 0001-527X ...
Cole TB, Jansen K, Park S, Li W-F, Furlong CE, Costa LG. The toxicity of mixtures of specific organophosphate compounds is modulated by paraoxonase 1 status. In: Reddy ST, ed. Paraoxonases in Inflammation, Infection, and Toxicology, Volume 660 of Advances in Experimental Medicine and Biology. New York, NY: Humana Press, 2010, Chapter 6, pp. 47-60 ...
Furlong CE, Li W-F, Cole TB, Jampsa R, Richter RJ, Jarvik GP, Shih DM, Tward A, Lusis AJ, Costa LG. Understanding the significance of genetic variability in the human PON1 gene. In: Valdes JJ, Sekowski JW, eds. Toxicogenomics and Proteomics, NATO Science Series: Life and Behavioural Sciences, Volume 356. Amsterdam, Netherlands: IOS Press, 2004 ...
Journal of Clinical and Diagnostic Research aims to publish findings of doctors at grass root level and post graduate students, so that all unique medical experiences are recorded in literature.
4-(3-Oxobutyl)phenyl acetate | C12H14O3 | CID 19137 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
Association, Gene, Patients, Population, Health, Allele, Diseases, Risk, Factor Xiii, Socioeconomic Status, Disease, Human, Humans, Kidney, Kidney Disease, Lipoprotein, Paraoxonase, Paraoxonase-1, Serum, Transplant
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Žilinský samosprávny kraj (ŽSK) sa rozhodol ponúknuť deväť budov bývalých polikliník súkromným lekárom v snahe motivovať ich, aby v budovách zostali naďalej poskytovať zdravotnú starostlivosť
eeh tetibe tersampai kat Bukit Tinggi plak eh? memang pon dah plan nak pergi sini lepas event tu.. sebelum ni memang mengidam sangat lah kan nak pergi Bukit Tinggi.. terpedaya dengan gambar2 orang... cantek gile.. ala2 perancis gituuu.. tapi cukup SEKALI ni jek!!! tak mahu pergi lagi... biase2 jek rupenya.. barang2 pon mahal gilo.. saket kaki jek jalan sini..just cantek untuk bergambar jek kot.. nak naek ke sini pon tak best..jalan die jalan kecik..hutan memanjang..ak dah pikir sesat ke hape ni..seb baek betul memang jalan ke bukit tinggi.. pening kepala jangan cakap ahh kan..sumpah pening! ak buat2 sehat jek depan hanis..supaya die tak give up memanjat bukit.. muahahhaaa!!! eh tapi kalau korang nak pergi, silakan lah pergi..harap2 tak menyesal macam ak.. ...
Probab=100.00 E-value=0 Score=636.12 Aligned_cols=238 Identities=42% Similarity=0.741 Sum_probs=223.6 Q ss_pred HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH Q ss_conf 99999999831231023222212468888751248999999999999999999999748999999999995302022433 Q gi,254780372,r 4 YCFFALFLVTPELVFAKSSLHDVMNIPADLSISTWIVRTFGIFTILSIAPILLIMVTCFPRFIIVFSILRTGMGMGSVPP 83 (246) Q Consensus 4 ~~~~~~~~~~~~~a~aq~~~~~~~~~~~~~~~~~~~iqll~llt~LsL~P~ilim~TsFtrIvIVLsilRnALG~QQ~PP 83 (246) T Consensus 140 ~~~~~~~~~~~p~~~~~~p~~~~~~~~~g~q~~S~~iQ~Li~lT~LsllPaiLiM~TSFtRIvIVLslLRnALG~QQ~PP 219 (379) T PRK12430 140 IIPLCFLLLFCPSAYADIPGVTSHILSDGSQTWSIPVQTLVFLTSLTFLPAFLLMMTSFTRIVIVFGLLRNALGTPYAPP 219 (379) T ss_pred HHHHHHHHHHCHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH T ss_conf 79799999967497853885215305899801230899999999999999999998520899999999987307688986 Q ss_pred HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCC Q ss_conf ...
Kdy se vloni objevil v kinech film Tom e Ma na 3 sezony v pekle, inspirovan autobiografick mi vzpom nkami undergroundov ho b sn ka a filozofa Egona Bondyho, zd lo se mi zaj mav zjistit, co o takov m obrazu sv ho otce soud jeho syn Zbyn k Fi er.
Viac informácií o benzol-1-carbonsaeure-4-sulfonsaeuredipropylamid. Umožňujeme pokrok vo vede tým, že ponúkame výber produktov, servisné služby, vedecké inovácie, neustále zlepšovanie a zameriavanie sa na zákazníka.
想知道關於什麼的眼睛常識呢?. 歡迎留言或是寫信到[email protected]》. 我們會定期撰寫相關的眼科衛教喔(. 眼睛有任何不適3請儘速就醫3本部落格文章無法取代醫師的專業診療》. ...
Resveratrol (3,4,5-trihydroxy-trans-stilbene) is a secondary plant metabolite which is highly abundant in red grape skin and red wine [1]. Other dietary sources of resveratrol comprise berries, and peanuts [2, 3]. Although controversially discussed it has been recently shown that resveratrol may increase life span in model organisms such as Caenorhabditis elegans and Drosophila melanogaster [4-6]. The underlying mechanisms by which resveratrol may mediate beneficial effects have yet not been fully understood and may be partly related to its ability to induce phase II and antioxidant enzymes including heme oxygenase-1 and paraoxonase-1.. Heme oxygenase-1 (HO-1), is an inducible enzyme that catalyzes the rate-limiting step in the oxidative degradation of cellular heme that liberates iron, carbon monoxide (CO), and biliverdin. HO-1 exhibits antioxidant, anti-inflammatory and other cytoprotective functions [7].. Paraoxonase-1 (PON1) is a HDL associated serum enzyme which is mainly synthesized in ...
[4-(1-benzothiophen-2-yl)phenyl] acetate 132932-62-8 safety info, [4-(1-benzothiophen-2-yl)phenyl] acetate chemical safety search, Chemical [4-(1-benzothiophen-2-yl)phenyl] acetate safety technical specifications ect.
AIM: Bipolar disorder (BD) has a complex genetic etiology, with multiple unidentified genes and environmental factors playing important roles in its pathogenesis. A growing body of evidence suggests that reactive oxygen species (ROS) may be crucially involved in the pathogenesis of psychiatric diseases, including BD. The association between paraoxonase 1 (PON1), an important antioxidant enzyme, and development of BD has been scarcely investigated. We thus attempted to examine genetic variants in the PON1 gene, a putative BD susceptibility gene, in patients with bipolar disease and their first-degree relatives.. MATERIALS AND METHODS: The study population consisted of 292 healthy individuals, 199 patients with BD, and 280 unaffected first-degree relatives of the patients. Genotyping of PON1 L55M and Q192R polymorphisms was performed by polymerase chain reaction and restriction enzyme digestion.. RESULTS: Patients mostly shared the same PON1 genotypes with their first-degree relatives. The ...
Paraoxonase 2 (PON2) is a lactonase and a member of the paraoxonase family of genes (31). PON2 also has an important role in the development of atherosclerosis (32). PON2 is found in a variety of species including, mice, rats, monkeys and humans and is expressed all over the system. PON2 has been demonstrated to have an antioxidant effect, and is suggested to play a major role in preventing the development of atherosclerosis and also in a neuroprotective role (33-35). Females in various species also tend to have more PON2 than males.. The location of PON2 in the mitochondria strongly suggests that this enzyme has a role in protecting the cell from oxidative damage, indeed PON2 deficiency causes mitochondrial dysfunction (36). It is also interesting that the mitochondria, nucleus and cytoplasm are all areas of prefered accumulation for quercetin within the cell (37). We also know from experiments using human endothelial cells that PON2 can reduce the release of superoxide from the inner ...
4-(1-Adamantyl)phenyl acetate | C18H22O2 | CID 269932 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
3-hydroxy-13-amino-13,17-secoandrostan-17-oic-13,17-lactam p-bis(2-chloroethyl)amino phenyl acetate: an alkylating agent for treating breast cancer; lactandrate is the (5alpha)-isomer; structure in first source
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. . ...
Sheesh! Hari tu baru lepas menjawab paper Foundation engineering. Haih. Ape nak cakap. Dah buat banyak latihan dan sesi menghafal formula secara intensif dan agresif pon masih juga lupa. Kepada Dr. Lee, I memang takle bla la paper u. Walaupon da tau ape yang perlu dipelajari tapi masih juga susah. Bekerja dengan alam semula jadi memang agak sukar sebab semuanya tidak boleh dijangka dengan tepat. Hence, walaupon menggunakan factor of saftey yang tinggi, masih juga gagal menjawab soalan. So how? Maybe usaha sir untuk buat soalan lebih intense dari masa revision I maka I sungguh sorry untuk itu =p tapi I memang tak terel la nak hafal2 ni. Dulu pon masa sekolah, hafazan time kelas agama pon paling boleh pegi pon B je ...
mak chemo utk 6cycle, every 21 days die kene chemo..(sori cecampur bahasa..) so dalam mase 4 bulan setengah mak dalam tempoh rawatan..lepas jek 2 minggu 1st chemo hari tuh, rambut mak da gugur sedikit, mak skang dah 2nd cycle chemo, rambut die pon da makin banyak gugur..mmg bertabur dalam umah..hari2 kene menyapu rambut mak...pepe pon mak maintain cute..mak saye cumelll.. mak pon tak blei terdedah cahaya matahari, die akan rase bahang yang teramat..rase terbakat kulit..pergelangan tangan n muke mak yang terdedah n cahaya pon da makin menggelap ...
hempp.. rasanya Aida pon dah tahu citer pasal kite kan? Julie lagi sedih.. at least Aida ada banyak susu.. boleh dipam.. Julie pon ceasar jugak mcm Aida.. & nurse2 kat hospital tu tak spend banyak masa utk ajar Julie breastfeedkan baby.. maybe dia ingat kita dah reti.. memula dlm kepala pk anak2 ni mmg akan minum susu dari puting ibunya no matter what.. tapi sebenarnya tak.. masa lepas bersalin, rupanya si hubby dah benarkan nurse bagi susu botol kat Aisya sbb Aisya dah lapar.. sedangkan Julie still tak keluar dari OT.. patutla dia tanak isap puting walaupon dipujuk.. lepas balik dari hospital, sampai je rumah, hati mula runsing.. Julie dah takde mak nak tunjuk macamana nak breastfeed.. mak mertua pon tak datang lagi time tu.. takut sangat baby terjaga dari tidur & minta susu.. at last dia bangun jugak & menangis kelaparan.. yang ada hanyalah Julie & hubby.. bila disua puting dia tak reti nak isap.. nak pegang baby pon belum pandai.. sampaila sejam dia nangis.. lastly Julie give up & minta hubby ...
My MIL pon mcm kurg percaya anak dia leh jg baby.heh.Tapi saya percaya suami saya.Dari anta nursery kan.Ntah2 takde org syg pon kat anak saya kat situ :-P Tapi masa mula2 dulu pon azwan mmg susah nak bgn malam buat susu.Susah nak sedar dr tido kalau Rayna nangis.Tapi btol la org kata,masa bole merubah segalanya ...
V poslednom čase si mnohí z nás uvedomujú ako naše životy ovplyvňuje chémia. Potraviny, oblečenie, produkty dennej potreby, ale i životné prostredie je plné škodlivín. Ak si vážite svoje zdravie či zdravie svojich blízkych, milujete prírodu a chcete naozaj žiť kvalitne, ste tu správne. Bioprodukty, ktoré vám ponúkame sú ideálnym riešením pre tých, ktorí sa rozhodli uprednostniť alternatívu prírody pred syntetikou. Sme dôslední v ich výbere a preto dbáme aby každý bioprodukt mal certifikát. Ten vám zaručí výnimočnú kvalitu a ponúka istotu, že dostanete produkt, ktorý neobsahuje zbytočnú chémiu. Všetky certifikované bioprodukty sú označené symbolom organizácie, ktorá ich certifikovala. Náš sortiment sa neustále rozširuje a jeho portfólio tvoria biovýrobky renomovaných firiem. Všetky patria medzi etických výrobcov, dodržujú princíp fair trade a snažia sa minimalizovať negatívny dopad výrobného procesu na životné ...
Kvety pre Bejrút a ponúka rovnaký deň doručenia kvetín pre Bejrút na veľmi nízkej úrovni. Ponúkame kytice z 23.00 {AKT} | 18.87 EUR | 16.64 GBP s rovnaký deň doručenia. Otvorené 7 dní v týždni · Bezpečný nákup · Najnižšie ceny · Dodávka v rovnaký deň · 100% čerstvé kvety · Platba prostredníctvom CC alebo Paypal · Podpora telefónu
Objectives: Hypogonadism is associated with a higher risk of atherosclerosis and cardiovascular disease, but the mechanisms underlying this association are not yet fully understood. Increased oxidative stress has been associated with development of cardiovascular and cerebrovascular diseases. We aimed to determine serum PON1 activities, an established lipid antioxidant, MDA levels which are end products of lipid peroxidation induced by ROS and thiol groups as an antioxidant for evaluating oxidative stress in patients with hypogonadism before and after replacement therapy.. Design and methods: A total of 18 male patients with untreated idiopathic hypogonadotropic hypogonadism (mean age: 27.4±7.3 years) and age, sex, and weight matched eugonadal healthy subjects (mean age: 32.6±7.2 years) were enrolled in the study as a control group. Serum PON1 activity, MDA and thiol levels were measured according to an enzymatic spectrophotometric method.. Results: Serum MDA and thiol levels were higher and ...
RESULTS Compared with whites, there were high frequencies of the AGT TT genotype in Chinese control subjects (120/183 = 70%) and type 2 diabetic patients (74/110 = 67%). The frequencies of the MM genotype were 5 and 3%, respectively, and those of the ACE DD genotype were 13 and 10%, respectively. Although 9% of subjects carried the C allele, the AT1 CC genotype was not found in either group. Chinese type 2 diabetic patients with increased albuminuria (n = 56) had higher systolic blood pressure (160 ± 26 mmHg vs 145 ± 27 mmHg, P , 0.001) than the normoalbuminuric patients (n = 54). Both the AGT TT genotype (78.6% [44/56] vs. 55.6% [30/54], odds ratio [OR]: 3.0 [1.3-6.8]) and the T allele (88% [99/112] vs. 77% [83/108], OR: 2.5 [1.3-5.4]) were associated with an increased risk of albuminuria. Patients with the AGT TT genotype (n = 74) had higher 24-h UAE than those with the MT or MM genotypes (n = 36) (median: 37.8 mg/day vs. 17.8 mg/day, P , 0.01). This association remained significant in ...
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.. ...
Yarrrgh a general cry ta muster thee Pyrates fer thee visit of our hesteemed Pirate colleague (although he dont often post ere on haccount of his Hamerikan haccent) Capn Qwerty. It looks loik we could be meetin up in ye Rychmond Hupon Ye Thames, Surrey, on Ye Saturday comin loik...although I could be wrong ...
Krásna kytica červených a bielych ruží vám prinesie nádherný darček! Kvety pre Bejrút a ponúka rovnaký deň doručenia kvetín pre Bejrút na veľmi nízkej úrovni. Ponúkame kytice z 23.00 {AKT} | 18.87 EUR | 16.64 GBP s rovnaký deň doručenia. Otvorené 7 dní v týždni · Bezpečný nákup · Najnižšie ceny · Dodávka v rovnaký deň · 100% čerstvé kvety · Platba prostredníctvom CC alebo Paypal · Podpora telefónu
Červené ruže a jedna biela ruža v strede obklopené zeleňou. Dokonalá... Kvety pre Mijas / Mijas Costa a ponúka rovnaký deň doručenia kvetín pre Mijas / Mijas Costa na veľmi nízkej úrovni. Ponúkame kytice z 8.00 {AKT} | 9.32 USD | 7.01 GBP s rovnaký deň doručenia. Otvorené 7 dní v týždni · Bezpečný nákup · Najnižšie ceny · Dodávka v rovnaký deň · 100% čerstvé kvety · Platba prostredníctvom CC alebo Paypal · Podpora telefónu
你知道光網路"last mile" PON 的最新演進與測試需求嗎?想瞭解超高速網路40/100GbE 測試祕密嗎? Intel lightPeak 平台又是什麼樣的傳輸平台呢? 只要參加2011 Anritsu光論壇,三個願望, 一次滿足!. 本次由安立知與電機電子公會主辦的研討會,將以Next Generation PON、40G/100G Ethernet以及Optical Interconnect等光通訊技術為主題,介紹最新概況與量測解決方案。邀請工研院資通所 徐達儒副理與Alcatel-lucent 馮國璋技術經理,以不同角度探討XG-PON 發展;以及安立知日本市場部經理 近本 寬和先生與各位分享最新40G/100G市場與技術趨勢;另有國內專業學者中央大學 伍茂仁教授蒞臨講解Optical Interconnect相關技術,現場還有實機展示,歡迎各位先進踴躍報名參加!. 議程:. ...
padahal org lain dah bertimbun dpt award..xkecoh pon..sbb br sket la kita nak kecoh kan..yg dah byk,bnda 2 da biase...hehehe...pape hal pon, tQ to my dear tik coz gave me this award..syg u ketat-ketat dear ...
perl use strict; use warnings; use Test::More; use Statistics::R; SKIP: { skip because tests hang on Win32 (bug #81159), 1 if $^O =~ /^(MS) +?Win32$/; ok my $R = Statistics::R-,new(); is $R-,run(q`a ,- 1;`), ; eval { $R-,run( qq`print(Hello);\nprint(ASDF)` ); }; #diag Diagnostic: \[email protected]\n; ok [email protected], Runtime error; is $R-,run(q`a ,- 1;`), ; ok $R = Statistics::R-,new(); eval { $R-,run( qq`print(Hello);\nprint ASDF` ); }; #diag Diagnostic: \[email protected]\n; ok [email protected], Syntax error; # Actual error message varies depending on locale is $R-,run(q`a ,- 1;`), ; use_ok t::FlawedStatisticsR; ok $R = t::FlawedStatisticsR-,new(); eval { $R-,run( qq`print(Hello);\ncolors,-c(red)` ); }; #diag Diagnostic: \[email protected]\n; ok [email protected], Internal error; # THIS IS LINE 40 !!! }; done_testing ...
Hygiena p edko kov ho vaku - Na aludu a vnit n stran p edko ky se mi tvo jak si b l ka i kovit povlak, kter pon kud zap ch . Po umyt aludu se v ak vytvo povlak nov . N kdy je m j alud natolik podr d n , e p i jeho myt poci uji velice silnou ezavou bolest. Mohlo by se jednat o n jak z n t? Pora te mi pros m jak se toho zbavit... poradna zdravi predkozkovy vak, Zdravotn poradna
live the jobless life to the fullest! berdebar jugak nak tunggu kerja dan takut akan dicampak ke mana laaaahhh nanti dengan perut memboyot ni. boyot lah sangat. sumorang cakap perut kecik taknampak pon mcm pregnant. dah boncit sangat okaiiii. haha ...
live the jobless life to the fullest! berdebar jugak nak tunggu kerja dan takut akan dicampak ke mana laaaahhh nanti dengan perut memboyot ni. boyot lah sangat. sumorang cakap perut kecik taknampak pon mcm pregnant. dah boncit sangat okaiiii. haha ...
Obchodným partnerom ponúkame v súčasnosti viac ako 160 druhov kvapalných a práškových prípravkov, každý v rôznych hmotnostných veľkostiach balenia.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Arsenic atom in PDB 2o4m: Structure of Phosphotriesterase Mutant I106G/F132G/H257Y
Shop Lactonase ELISA Kit, Recombinant Protein and Lactonase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
The ribonuclease (RNase) molecule which takes part in the formation of enzyme-substrate complexes, was investigated to determine factors which affect the binding of various competitive inhibitors of RNase activity. The binding of inhibitory nucleotides, such as 2-cytidylic acid, by RNase was measured not only by enzyme inhibition, but also by spectral changes and dialysis equilibrium. As measured spectrophotometrically, complex formation between nucleotideAND RNase is manifested by changes in the spectral contributions from both pyrimidine and tyrosyl groups. The affinities of RNase for 2-cytidylic acid as measured by all 3 methods were in excellent agreement. As measured by dialysis equilibrium and spectral changes, only one molecule of nucleotide is bound per molecule of enzyme. Since 2-cytidylic acid is a competitive inhibitor of both catalytic actions (transferase and hydralase) of the enzyme, it may be inferred that the same catalytic site is responsible for both reactions. (Author)
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hari ni i bosan, i xtau nk wtpe so i lepak bendahari dgn 2 bebuncet yg sengal ni..tp i bosan jugak..sume org wt keje tp i mls nk wt keje tp i bosan..i tgk kiri kanan pndg org sana sini tp xde sorg pon pndg i balik..i bosan..sape pon xmngerti kehendak jiwa raga i..i bkak fon lame i, fon baru i masuk sungai hntar msuk ward smpai arini xkua2 lg..fon lame i buruk, malu nk kluarkn tp i xde fon laen..itu jelah satu..i keluarkn senyap2 dri beg i..i bukak flip dia, i pegi application..i msuk FaceWarp..lps tu terjadi lah perkara2 yg xsepatutnya seperti ini..i suke.. i suke..hooyehh hoooyyeeehhhh!! ...
Nafsu nak bershopping tu sebenarnya x dela sgt... tambahan en shahir plak JB hari ni sampai esok... tujuan utamanya nak cari Mamy poko jer... sbb seigt igt saya.. mmg murah.. pernah pergi sekali J-card day dulu... dan dulu pon saya gi lepas maghrib... mamy poko masih byk... brg2 sale seperti diatas dia x kan letak kat tpt biasa... diorang akan longgokkan kat entrance dan kita hanya beratur dan terus byr kat kaunter special kat situ... so saya plan gitu ajerla...dah amik mamy poko..kita balik ler... tp kalau nak nyinggah2 sat tempat lain pon apa salahnyer~~~ hehehe ...
Webov port l predaj-k pa.sk pon ka mno stvo bezplatn ch inzer tov z cel ho Slovenska a eskej Republiky. Av za a inzer ty v novin ch u nie s v m de, web je t prav cesta kde m ete v hodne pred va i nakupova .
Ponúkame kvalitné mikroskopy od svetových výrobcov, mikroskopy pre školy, mikroskopy pre laboratória, mikroskopy za skvelé ceny.
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0050] Experiments in DIO mice have also provided insights in to the effects of cholesterol loading on macrophage function in the eye. Macrophages fed a high fat diet for a period of up to 6 months are unable to regulate CNV in vivo and vascular endothelial cell proliferation in vitro. These findings shed light on conflicting data from genetic and epidemiologic studies that demonstrate a complex association between polymorphisms in genes that regulate HDL and cholesterol metabolism and AMD as outlined above (Chen, W. et al. Genetic variants near TIMP3 and high-density lipoprotein-associated loci influence susceptibility to age-related macular degeneration. Proc Natl Acad Sci USA 107, 7401-7406, 2010; Neale, B. M. et al. Genome-wide association study of advanced age-related macular degeneration identifies a role of the hepatic lipase gene (LIPC). Proc. Natl. Acad. Sci. USA 107, 7395-7400, 2010). The data from knockout mice and the reduced expression of ABC transporters from old humans, diagnosed ...
Although Pichia pastoris has been successfully used to produce various recombinant heterologous proteins, the efficiency varies. In this study, we used methyl parathion hydrolase (MPH) from Ochrobactrum sp. M231 as an example to study the effect of protein amino acid sequence on secretion from P. pastoris. The results indicated that the protein N-terminal sequence, the endoplasmic reticulum (ER) retention signal (KKXX) at the protein C-terminus, and the acidic stability of the protein could affect its secretion from P. pastoris. Mutations designed based on these sequence features markedly improved secretion from P. pastoris. In addition, we found that the secretion properties of a protein can be cumulative when all of the above strategies are combined. The final mutant (CHBD-DQR) designed by
P.509 left column 3rd paragraph: Mitochondria were isolated from cell suspensions according to the no gradient method of Bogenhagen and Clayton (Bogenhagen and Clayton, 1974). P.509 left column bottom paragraph: Quantitation of mtDNA: Mitochondrial DNA (mt DNA) was measured using the method of Tapper and Clayton (Murphy et al., 1984). P.509 right column 2nd paragraph: Mitochondrial counting: Isolated mitochondria from all cell types were stained with Rhodamine 123 according to the method of Johnson, Walsh, and Chen (Johnson et al., 1980). P.509 right column 3rd paragraph: Enzyme assays: Cytochrome aa3 was measured spectrophotometrically by the method of Simon et al. (1973). P.509 right column 4th paragraph: Malate dehydrogenase: Malate dehydrogenase was measured spectrophotometrically by the method of Rudolph, Baugher, and Beissner (Rudolph et al., 1977 ...
Pafah1b2 - Pafah1b2 (untagged) - Mouse platelet-activating factor acetylhydrolase, isoform 1b, subunit 2 (Pafah1b2), (10ug) available for purchase from OriGene - Your Gene Company.
uh. seriously, aku memang tak sangka. yelah. nak kate aku dapat rasa simptom simptom awal penyakit neyh tak de pon. aku tak rasa pape pon. dan yang lebih mengundang kekecewaan adelah.... nenek aku yang gigi pon tak sampai separuh, colour rambut di dye kan dengan warna putih dan sedikit kelabu xde pon penyakit neyh. siap boleh eja name aku penuh lagi. kenapalah aku yang ade. T__T kalaulah diorang tahu, mesti mereka gelakkan aku sampai terguling guling. uh. rasa tak nak balik raya je tahun neyh. emm. bila pikir pikir balik, mungkinkah ni petanda aku akan mati awal? oh. amalan pon tak sampai satu sudu. berbakti dekat mak bapak pun belum. kahwin pon belum. apetah lagi nak rasa ubun ubun baby sendiri. mintak mintak lah dijauhkan segala perkara buruk. ...
1ES9: The functional implications of the dimerization of the catalytic subunits of the mammalian brain platelet-activating factor acetylhydrolase (Ib).
hai dila..sy tulun jwbkn la ea..hihi..aritu sy de p cari brg2 cross stitch..lau nk cari benang n brg2 die tu cube jln ke depan sket dr semua house tu..cari cimb n maybank..ade kedai barang alat2 jahitan n brang2 hntaran sebelah je ngan cimb tu..kdai tu agak lengkap la ..benang pon bnyk pilihan..cuma dr segi harga tu standard RM1.80..ade lg satu kedai jual brg2 hantaran n bunga2 menghala ke masjid india yg kaler oren tu pon de jual gak brg2 cross stitch n benang..cuma dr segi hrga kain tu, kt kdai ni lebih mahal..tp hrga benang sama je..ha kt level bwh semua house tu pon ade gak..benang dmc cume rm1.20 je tp xbnyk pilihan gat..n brg2 len cam kain pon xde dijual..dlm ketiga-tiga kedai nih, cuma yg kedai menghala ke masjid oren tu je ade jual pattern..tp tu pon pattern lama..cube cari pattern online..bnyk gak yg latest n lawa..hope info nih membantu..hihi..^___^)v~. ReplyDelete ...
View Notes - 04 Notes - Naming Acids and Bases from CHEM 101 at Long Island U.. Aim: How are acids and bases named? QQ: W hat is an amphoteric substance? Do Now: How can you determine if a substance
One and a half months later, he emailed me the draft (after being reminded by the in-laws). My..oh my...terkejut i tengok the draft. i dun like the tone of the colour, ejaan nama sume salah, map pon suruh buat yang baru (grhhhh!!!..penat2 i buat then senang2 minta buat yg baru!!), and the design is not similar to what we have discussed earlier..it was very dissapointing :( Being very naive, i never thought that i wud face this kinda problem..dalam keadaan yang dah genting, i decided to go for a local vendor who can custom made my wedding invitation within this short period of time..but who??...*sedih and stress..deposit pon burnt macam tu je ...
Polymorphisms in paraoxonase 1 (PON1) coding for PON1 enzyme have been studied as genetic markers of coronary artery disease (CAD). PON1 Q192R and PON1 L55M polymorphisms have been analyzed extensively, but data on association and role of these polymorphisms in the etiology of CAD are conflicting. In this study, we tested the genetic association between PON1 Q192R and PON1 L55M polymorphisms and CAD among north Indians. MATERIALS AND METHODS: Two hundred eighty-five angiographically proven patients with coronary artery disease and 200 sex-matched and ethnically matched controls were genotyped for 2 PON1 polymorphisms by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Genotype/ allele frequencies were compared in patients and controls using the chi-square test. RESULTS: At PON1-192 locus, there were significant differences between patients and controls (P, 0.05), leading to significant odds ratios for RR genotype (OR= 1.92, CI: 1.19-3.10) and *R allele ...
Interleukin-7 (IL-7) is a critical factor for T cell development and for maintaining and restoring homeostasis of mature T cells. Polymorphisms at α-chain of the IL-7 receptor (IL7R or CD127) gene are related to evolution of HIV-infection, but there are no data concerning the evolution of hepatitis C virus (HCV) infection. The aim of this study was to analyze the association between IL7R polymorphisms and severe liver disease in HCV/HIV coinfected patients. We performed a cross-sectional study in 220 naïve patients who underwent a liver biopsy. IL7R polymorphisms (rs6897932, rs987106 and rs3194051) were genotyped using the GoldenGate® assay. The outcome variables were: (a) liver biopsy: advanced fibrosis (F ≥ 3), severe activity grade (A3); (b) non-invasive indexes: advanced fibrosis (APRI ≥1.5 and FIB-4 ≥3.25). Logistic regression analysis was used to investigate the association between IL7R polymorphisms and outcome variables. This test gives the differences between groups and the odds ratio
Dietary supplementation with polyphenolic antioxidants to animals was shown to be associated with inhibition of LDL oxidation and macrophage foam cell formation, and attenuation of atherosclerosis development. We investigated the effects of pomegranate juice (PJ, which contains potent tannins and anthocyanins) consumption by atherosclerotic patients with carotid artery stenosis (CAS) on the progression of carotid lesions and changes in oxidative stress and blood pressure. Ten patients were supplemented with PJ for 1 year and five of them continued for up to 3 years. Blood samples were collected before treatment and during PJ consumption. In the control group that did not consume PJ, common carotid intima-media thickness (IMT) increased by 9% during 1 year, whereas, PJ consumption resulted in a significant IMT reduction, by up to 30%, after 1 year. The patients serum paraoxonase 1 (PON 1) activity was increased by 83%, whereas serum LDL basal oxidative state and LDL susceptibility to copper ...
We hypothesized that impaired protection of HDL against apoB-containing lipoprotein oxidation in the 11.1 transgenic mouse model could play a role in its enhanced atherosclerosis susceptibility. This line of human apoA-II in transgenic mice presented a relative increase in the area stained with antibodies that recognize LDL oxidation epitopes compared with control or 25.3 mice. However, a concomitant increase in plasma markers of oxidative stress was not found, which suggests that the level of oxidation of plasma lipoproteins was similar in the three mouse lines studied. Thus, the main difference in oxidation susceptibility may occur in the subendothelial space. In this milieu, the antioxidant and anti-inflammatory properties of HDL may confer vital protection.38. Lipoproteins that undergo oxidation are known to increase their electrophoretic mobility. This property can be used to establish the degree of LDL oxidation and has been used previously to assess HDL protectivity.39 Using this ...

Aryldialkylphosphatase - WikipediaAryldialkylphosphatase - Wikipedia

Aryldialkylphosphatase (EC 3.1.8.1) (more commonly known as phosphotriesterase, and also organophosphate hydrolase, parathion ...
more infohttps://en.wikipedia.org/wiki/Aryldialkylphosphatase

Pon1 - Serum paraoxonase/arylesterase 1 - Mus musculus (Mouse) - Pon1 gene & proteinPon1 - Serum paraoxonase/arylesterase 1 - Mus musculus (Mouse) - Pon1 gene & protein

aryldialkylphosphatase activity Source: UniProtKB. *arylesterase activity Source: MGI ,p>Inferred from Mutant Phenotype,/p> ,p> ...
more infohttp://www.uniprot.org/uniprot/P52430

Effect of flaxseed supplementation and exercise training on lipid profile, oxidative stress and inflammation in rats with...Effect of flaxseed supplementation and exercise training on lipid profile, oxidative stress and inflammation in rats with...

Aryldialkylphosphatase / blood. Biological Markers / metabolism. Combined Modality Therapy. Dietary Supplements*. Disease ... 0/Biological Markers; 0/Inflammation Mediators; 0/Lipids; 0/Lipoproteins; 0/Troponin I; EC 3.1.8.1/Aryldialkylphosphatase; EC ...
more infohttp://www.biomedsearch.com/nih/Effect-flaxseed-supplementation-exercise-training/23036047.html

Diethyl 4-Methylbenzylphosphonate - DrugBankDiethyl 4-Methylbenzylphosphonate - DrugBank

Aryldialkylphosphatase activity. Gene Name. opd. Uniprot ID. P0A434. Uniprot Name. Parathion hydrolase. Molecular Weight. ...
more infohttps://www.drugbank.ca/drugs/DB02138

Triethyl Phosphate - DrugBankTriethyl Phosphate - DrugBank

Aryldialkylphosphatase activity. Gene Name. opd. Uniprot ID. P0A434. Uniprot Name. Parathion hydrolase. Molecular Weight. ...
more infohttps://www.drugbank.ca/drugs/DB03347

Anti-PON2 antibody (ab230503) | AbcamAnti-PON2 antibody (ab230503) | Abcam

Rabbit polyclonal PON2 antibody. Validated in WB, IHC, Flow Cyt and tested in Human. Immunogen corresponding to synthetic peptide.
more infohttps://www.abcam.com/pon2-antibody-ab230503.html

Anti-PON2 antibody (ab40969) | AbcamAnti-PON2 antibody (ab40969) | Abcam

Rabbit polyclonal PON2 antibody validated for WB, IHC, ICC/IF and tested in Human and Mouse. Referenced in 2 publications and 1 independent review. Immunogen…
more infohttp://www.abcam.com/pon2-antibody-ab40969.html

OriGene - PON2 (NM 001018161) Human ORF cDNA CloneOriGene - PON2 (NM 001018161) Human ORF cDNA Clone

Synonyms: A-esterase 2; aromatic esterase 2; paraoxonase nirs; serum aryldialkylphosphatase 2; serum paraoxonase/arylesterase 2 ...
more infohttp://www.origene.com/orf_clone/trueclone/NM_001018161/RC222678/PON2.aspx

Loughborough University Institutional Repository: Paraoxonase 1 GENE polymorphisms contribute to coronary artery disease riskLoughborough University Institutional Repository: Paraoxonase 1 GENE polymorphisms contribute to coronary artery disease risk

Aryldialkylphosphatase. Coronary artery disease. Genetic. Genetic predisposition to disease. Genotype. Human. Polymorphism. ...
more infohttps://dspace.lboro.ac.uk/dspace-jspui/handle/2134/5347

PON1 protein, human - Semantic ScholarPON1 protein, human - Semantic Scholar

Serum paraoxonase/arylesterase 1 (355 aa, ~40 kDa) is encoded by the human PON1 gene. This protein plays a role in the catabolism of aromatic carboxylic acid esters, organophosphates and lactones.
more infohttps://www.semanticscholar.org/topic/PON1-protein%2C-human/621699

Paraoxonase - WikipediaParaoxonase - Wikipedia

Paraoxonases are a family of mammalian enzymes with aryldialkylphosphatase activity. There are three paraoxonase isozymes, ...
more infohttps://en.wikipedia.org/wiki/Paraoxonase

Hagai Tavori - Publications
     - Oregon Health & Science UniversityHagai Tavori - Publications - Oregon Health & Science University

Farbstein, D., Blum, S., Pollak, M., Asaf, R., Viener, H. L., Lache, O., Asleh, R., Miller-Lotan, R., Barkay, I., Star, M., Schwartz, A., Kalet-Littman, S., Ozeri, D., Vaya, J., Tavori, H., Vardi, M., Laor, A., Bucher, S. E., Anbinder, Y., Moskovich, D. & 4 othersAbbas, N., Perry, N., Levy, Y. & Levy, A. P., Nov 2011, In : Atherosclerosis. 219, 1, p. 240-244 5 p.. Research output: Contribution to journal › Article ...
more infohttps://ohsu.pure.elsevier.com/en/persons/hagai-tavori/publications/

Cancer Institute, Mechanisms of Carcinogenesis - Research Output
     - Penn StateCancer Institute, Mechanisms of Carcinogenesis - Research Output - Penn State

Furlong, C. E., Costa, L. G., Hassett, C., Richter, R. J., Sundstrom, J. A., Adler, D. A., Disteche, C. M., Omiecinski, C. J., Chapline, C., Crabb, J. W. & Humbert, R., Jan 1 1993, In : Chemico-Biological Interactions. 87, 1-3, p. 35-48 14 p.. Research output: Contribution to journal › Article ...
more infohttps://pennstate.pure.elsevier.com/en/organisations/cancer-institute-mechanisms-of-carcinogenesis/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Farticle&page=75

Hagai Tavori - Publications
     - Oregon Health & Science UniversityHagai Tavori - Publications - Oregon Health & Science University

Farbstein, D. , Blum, S. , Pollak, M. , Asaf, R. , Viener, H. L. , Lache, O. , Asleh, R. , Miller-Lotan, R. , Barkay, I. , Star, M. , Schwartz, A. , Kalet-Littman, S. , Ozeri, D. , Vaya, J. , Tavori, H. , Vardi, M. , Laor, A. , Bucher, S. E. , Anbinder, Y. , Moskovich, D. & 4 others Abbas, N., Perry, N., Levy, Y. & Levy, A. P. Nov 2011 In : Atherosclerosis. 219, 1, p. 240-244 5 p.. Research output: Contribution to journal › Article ...
more infohttps://ohsu.pure.elsevier.com/en/persons/hagai-tavori/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Farticle

PON2 antibody | pab0552-P | Covalab - Covalab BiotechnologyPON2 antibody | pab0552-P | Covalab - Covalab Biotechnology

Serum aryldialkylphosphatase 2. Gene name: PON2 Serum paraoxonase/arylesterase 2 (PON2) antibody ...
more infohttps://www.covalab.com/serum-paraoxonase-arylesterase-2-pon2-antibody-4.html

Microbial Metabolism of Organophosphates: Key for Developing Smart Bioremediation Process of Next Generation | Springer for...Microbial Metabolism of Organophosphates: Key for Developing Smart Bioremediation Process of Next Generation | Springer for...

Mulbry WW (1992) The aryldialkylphosphatase-encoding gene adpB from Nocardia sp. strain B-l: cloning, sequencing and expression ...
more infohttps://rd.springer.com/chapter/10.1007%2F978-981-15-2679-4_14

Egon Anderson Ozer - Fingerprint
     - Northwestern ScholarsEgon Anderson Ozer - Fingerprint - Northwestern Scholars

Fingerprint Dive into the research topics where Egon Anderson Ozer is active. These topic labels come from the works of this person. Together they form a unique fingerprint. ...
more infohttps://www.scholars.northwestern.edu/en/persons/egon-anderson-ozer/fingerprints/?sortBy=alphabetically

A common mutation in paraoxonase-2 results in impaired lactonase activity<...A common mutation in paraoxonase-2 results in impaired lactonase activity<...

TY - JOUR. T1 - A common mutation in paraoxonase-2 results in impaired lactonase activity. AU - Stoltz, David A.. AU - Ozer, Egon A.. AU - Recker, Thomas J.. AU - Estin, Miriam. AU - Yang, Xia. AU - Shih, Diana M.. AU - Lusis, Aldons J.. AU - Zabner, Joseph. PY - 2009/12/18. Y1 - 2009/12/18. N2 - Paraoxonases (PONs) are a family of lactonases with promiscuous enzyme activity that has been implicated in multiple diseases. PON2 is intracellularly located, is the most ubiquitously expressed PON, and has the highest lactonase activity of the PON family members. Whereas some single-nucleotide polymorphisms (SNPs) in PON1 have resulted in altered enzymatic activity in serum, to date the functional consequences of SNPs on PON2 function remain unknown. We hypothesized that a common PON2 SNP would result in impaired lactonase activity. Substitution of cysteine for serine at codon 311 in recombinant PON2 resulted in normal protein production and localization but altered glycosylation and decreased ...
more infohttps://www.scholars.northwestern.edu/en/publications/a-common-mutation-in-paraoxonase-2-results-in-impaired-lactonase-

Association between the paraoxonase-1 192Q|R allelic variant and coronary endothelial dysfunction in patients with early...Association between the paraoxonase-1 192Q|R allelic variant and coronary endothelial dysfunction in patients with early...

TY - JOUR. T1 - Association between the paraoxonase-1 192Q,R allelic variant and coronary endothelial dysfunction in patients with early coronary artery disease. AU - Lavi, Shahar. AU - McConnell, Joseph P.. AU - Lavi, Ronit. AU - Barsness, Gregory W.. AU - Rihal, Charanjit S.. AU - Novak, Gregory D.. AU - Lerman, Lilach O. AU - Lerman, Amir. PY - 2008. Y1 - 2008. N2 - OBJECTIVE: To test the hypothesis that allelic variants of the paraoxonase-1 gene are associated with endothelial dysfunction, an early stage of atherosclerosis. PATIENTS AND METHODS: We assessed 192Q,R and 55L,M allelic variants of the paraoxonase gene and coronary endothelial function in response to intracoronary acetylcholine in 99 patients (52 with homozygous QQ, 47 with homozygous RR or heterozygous QR). The study was conducted from September 1, 2002, through November 30, 2004. RESULTS: Of 52 homozygous QQ patients, 39 (75%) had endothelial dysfunction vs 20 (43%) of the 47 RR/QR patients (P=.001), and this association ...
more infohttps://mayoclinic.pure.elsevier.com/en/publications/association-between-the-paraoxonase-1-192qgtr-allelic-variant-and

Roberto Antonicelli - Research Output
     - Italian Ministry of HealthRoberto Antonicelli - Research Output - Italian Ministry of Health

Zanchetti, A., Bond, M. G., Hennig, M., Neiss, A., Mancia, G., Dal Palù, C., Hansson, L., Magnani, B., Rahn, K. H., Reid, J., Rodicio, J., Safar, M., Eckes, L., Ravinetto, R., Zanchetti, A., Bond, M. G., Dal Palù, C., Hansson, L., Hennig, M., Magnani, B. & 626 others, Mancia, G., Neiss, A., Rahn, K. H., Reid, J., Rodicio, J., Safar, M., Eckes, L., Ravinetto, R., Tong, R., Phillips, R. M., Alcorn, S., Angel, D., Craig, J., Holley, B., Li, M., Miller, T., Pozo, D., Rodriguez, M., Xiao, B., Wilson, C., Wolfe, M., Thomasson, B., Rohlmann, F., Flatau, B., Parati, G., Omboni, S., Ravogli, A., Villani, A., Santucciu, C., Ulian, L., Rappelli, A., Catalini, R., Zingaretti, O., Toutouzas, P., Pitsavos, C., Kallikazaros, I., Tsekoura, D., Tsioufis, K., Roca Cusachs, A., Negredo, E., Paytubì Garì, C., Scholze, J., Stuhr, G., Wolbart, C., Ambrosioni, E., Strocchi, E., Piccoli, M., Schiaratura, M., Agabiti Rosei, E., Bettoni, G., Muiesan, M. L., Gilfrich, H. J., Römer, A., Schüller-Mirza, K., Wintrich, ...
more infohttps://moh-it.pure.elsevier.com/en/persons/roberto-antonicelli/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Farticle&ordering=title&descending=true

Code System ConceptCode System Concept

Aryldialkylphosphatase (substance). Code System Preferred Concept Name. Aryldialkylphosphatase (substance). Concept Status. ...
more infohttps://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=130532007

Find Research Outputs
             - Albert Einstein College of MedicineFind Research Outputs - Albert Einstein College of Medicine

Calkins, H., Hindricks, G., Cappato, R., Kim, Y. H., Saad, E. B., Aguinaga, L., Akar, J. G., Badhwar, V., Brugada, J., Camm, J., Chen, P. S., Chen, S. A., Chung, M. K., Cosedis Nielsen, J., Curtis, A. B., Davies, D. W., Day, J. D., DAvila, AE., Natasja De Groot, N. M. S., Di Biase, L. & 50 othersDuytschaever, M., Edgerton, J. R., Ellenbogen, K. A., Ellinor, P. T., Ernst, S., Fenelon, G., Gerstenfeld, E. P., Haines, D. E., Haissaguerre, M., Helm, R. H., Hylek, E., Jackman, W. M., Jalife, J., Kalman, J. M., Kautzner, J., Kottkamp, H., Kuck, K. H., Kumagai, K., Lee, R., Lewalter, T., Lindsay, B. D., MacLe, L., Mansour, M., Marchlinski, F. E., Michaud, G. F., Nakagawa, H., Natale, A., Nattel, S., Okumura, K., Packer, D., Pokushalov, E., Reynolds, M. R., Sanders, P., Scanavacca, M., Schilling, R., Tondo, C., Tsao, H. M., Verma, A., Wilber, D. J., Yamane, T., Blomström-Lundqvist, C., De Paola, A. A. V., Kistler, P. M., Lip, G. Y. H., Peters, N. S., Pisani, C. F., Raviele, A., Satomi, K., Stiles, M. ...
more infohttps://einstein.pure.elsevier.com/en/publications/index/