Receptors, Aryl Hydrocarbon
Cytoplasmic proteins that bind certain aryl hydrocarbons, translocate to the nucleus, and activate transcription of particular DNA segments. AH receptors are identified by their high-affinity binding to several carcinogenic or teratogenic environmental chemicals including polycyclic aromatic hydrocarbons found in cigarette smoke and smog, heterocyclic amines found in cooked foods, and halogenated hydrocarbons including dioxins and polychlorinated biphenyls. No endogenous ligand has been identified, but an unknown natural messenger with a role in cell differentiation and development is suspected.
Aryl Hydrocarbon Hydroxylases
A large group of cytochrome P-450 (heme-thiolate) monooxygenases that complex with NAD(P)H-FLAVIN OXIDOREDUCTASE in numerous mixed-function oxidations of aromatic compounds. They catalyze hydroxylation of a broad spectrum of substrates and are important in the metabolism of steroids, drugs, and toxins such as PHENOBARBITAL, carcinogens, and insecticides.
Tetrachlorodibenzodioxin
Aryl Hydrocarbon Receptor Nuclear Translocator
Aryl hydrocarbon receptor nuclear translocator is a basic HELIX-LOOP-HELIX MOTIF containing protein that forms a complex with DIOXIN RECEPTOR. The complex binds xenobiotic regulatory elements and activates transcription of a variety of genes including UDP GLUCURONOSYLTRANSFERASE. AhR nuclear translocator is also a subunit of HYPOXIA-INDUCIBLE FACTOR 1.
Cytochrome P-450 CYP1A1
A liver microsomal cytochrome P-450 monooxygenase capable of biotransforming xenobiotics such as polycyclic hydrocarbons and halogenated aromatic hydrocarbons into carcinogenic or mutagenic compounds. They have been found in mammals and fish. This enzyme, encoded by CYP1A1 gene, can be measured by using ethoxyresorufin as a substrate for the ethoxyresorufin O-deethylase activity.
Polycyclic Hydrocarbons, Aromatic
A major group of unsaturated cyclic hydrocarbons containing two or more rings. The vast number of compounds of this important group, derived chiefly from petroleum and coal tar, are rather highly reactive and chemically versatile. The name is due to the strong and not unpleasant odor characteristic of most substances of this nature. (From Hawley's Condensed Chemical Dictionary, 12th ed, p96)
Procollagen-Proline Dioxygenase
A mixed-function oxygenase that catalyzes the hydroxylation of a prolyl-glycyl containing peptide, usually in PROTOCOLLAGEN, to a hydroxyprolylglycyl-containing-peptide. The enzyme utilizes molecular OXYGEN with a concomitant oxidative decarboxylation of 2-oxoglutarate to SUCCINATE. The enzyme occurs as a tetramer of two alpha and two beta subunits. The beta subunit of procollagen-proline dioxygenase is identical to the enzyme PROTEIN DISULFIDE-ISOMERASES.
Dioxins
Mixed Function Oxygenases
Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.
Benzo(a)pyrene
Hydrocarbons, Aromatic
Benz(a)Anthracenes
Hypoxia-Inducible Factor-Proline Dioxygenases
Steroid Hydroxylases
Environmental Pollutants
Enzyme Induction
Cytochrome P-450 Enzyme System
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
Hydroxylation
beta-Naphthoflavone
Benzoflavones
Organic compounds containing a BENZENE ring attached to a flavone group. Some of these are potent arylhydrocarbon hydroxylase inhibitors. They may also inhibit the binding of NUCLEIC ACIDS to BENZOPYRENES and related compounds. The designation includes all isomers; the 7,8-isomer is most frequently encountered.
Benzopyrenes
Hypoxia-Inducible Factor 1, alpha Subunit
Benzopyrene Hydroxylase
Alkanes
Polychlorinated Biphenyls
Basic Helix-Loop-Helix Transcription Factors
Hydrocarbons, Chlorinated
Xenobiotics
Hypoxia-Inducible Factor 1
Cytochrome P-450 CYP1A2
Alkane 1-Monooxygenase
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Ketoglutaric Acids
Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase
Aldehyde Oxidase
An aldehyde oxidoreductase expressed predominantly in the LIVER; LUNGS; and KIDNEY. It catalyzes the oxidation of a variety of organic aldehydes and N-heterocyclic compounds to CARBOXYLIC ACIDS, and also oxidizes quinoline and pyridine derivatives. The enzyme utilizes molybdenum cofactor and FAD as cofactors.
Dioxygenases
Liver
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Carcinogens
Phenylalanine Hydroxylase
Gene Expression Regulation, Enzymologic
Ligands
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
Cholestanetriol 26-Monooxygenase
Chloracne
Biodegradation, Environmental
Transcription Factors
Petroleum
Microsomes, Liver
Oxygenases
Amino Acid Sequence
Soil Pollutants
9,10-Dimethyl-1,2-benzanthracene
Steroids, Fluorinated
Gene Expression Regulation
Tryptophan Hydroxylase
Transcription, Genetic
Xanthine Dehydrogenase
Molybdenum
Oxygen
Base Sequence
Fundulidae
Water Pollutants, Chemical
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
DNA Adducts
Cells, Cultured
Reverse Transcriptase Polymerase Chain Reaction
Protein Binding
7-Alkoxycoumarin O-Dealkylase
Molecular Structure
DNA-Binding Proteins
Methylococcus
Dose-Response Relationship, Drug
Palladium
HSP90 Heat-Shock Proteins
Steroids, Chlorinated
Catalysis
Helix-Loop-Helix Motifs
Von Hippel-Lindau Tumor Suppressor Protein
Metabolic Detoxication, Drug
Binding Sites
Microsomes
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Mice, Knockout
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Hepatocytes
Biotransformation
The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
Structure-Activity Relationship
Transcriptional Activation
Gene Expression
Response Elements
Hydrocarbons, Alicyclic
Cloning, Molecular
Substrate Specificity
Alkenes
Hydrocarbons, Acyclic
Hydroxybenzoates
Promoter Regions, Genetic
Genes, Reporter
Beluga Whale
Growth Hormone-Secreting Pituitary Adenoma
Sequence Homology, Amino Acid
Receptor Cross-Talk
Environmental Monitoring
Blotting, Western
DNA Primers
Luciferases
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
A mitochondrial cytochrome P450 enzyme that catalyzes the 1-alpha-hydroxylation of 25-hydroxyvitamin D3 (also known as 25-hydroxycholecalciferol) in the presence of molecular oxygen and NADPH-FERRIHEMOPROTEIN REDUCTASE. This enzyme, encoded by CYP27B1 gene, converts 25-hydroxyvitamin D3 to 1-alpha,25-dihydroxyvitamin D3 which is the active form of VITAMIN D in regulating bone growth and calcium metabolism. This enzyme is also active on plant 25-hydroxyvitamin D2 (ergocalciferol).
Fuel Oils
Transfection
Receptors, Drug
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
Zebrafish
Hep G2 Cells
Pseudomonas putida
4-Hydroxybenzoate-3-Monooxygenase
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Gasoline
Mutation
Phenobarbital
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Cobalt
Carbazoles
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Oxidation-Reduction
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Nuclear Proteins
Hydroxyproline
Chromatography, High Pressure Liquid
Tumor Cells, Cultured
Isoenzymes
Proline
Oxidoreductases
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Models, Molecular
Fumarate Hydratase
Indoles
DNA, Complementary
Models, Biological
Cytosol
Glucuronosyltransferase
p-Aminoazobenzene
Enzyme Inhibitors
Ovulation Inhibition
Kaempferols
Intracellular Signaling Peptides and Proteins
Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.
Adipates
Pteridines
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
NAD(P)H Dehydrogenase (Quinone)
Coal Tar
A by-product of the destructive distillation of coal used as a topical antieczematic. It is an antipruritic and keratoplastic agent used also in the treatment of psoriasis and other skin conditions. Occupational exposure to soots, tars, and certain mineral oils is known to be carcinogenic according to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985) (Merck Index, 11th ed).
Mimosine
Binding, Competitive
COS Cells
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Gas Chromatography-Mass Spectrometry
Glutathione Transferase
Chromatin Immunoprecipitation
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Steroid 16-alpha-Hydroxylase
Flavin-Adenine Dinucleotide
Lung
Repressor Proteins
Flavins
Dimerization
Pharmacognosy
Antrodia
An investigation into the binding of the carcinogen 15,16-dihydro-11-methylcyclopenta[a]phenanthren-17-one to DNA in vitro. (1/3060)
After metabolic activation the carcinogen 15,16-dihydro-11-[3H]methylcyclopenta[a]phenanthren-17-one binds to DNA in vitro, and this binding is prevented by 7,8-benzoflavone. Radioactivity cannot be removed from the DNA with organic solvents or by chromatography on Sephadex G-50, even after heat denaturation of the DNA. Enzymatic hydrolysis yields radioactive fractions, which elute from a column of Sephadex LH-20 immediately after the natural nucleosides. At least two species of reactive metabolites are involved in this bending, those with a half-life of a few hr and others with greater stability. After extraction from the aqueous incubation mixture, they could be detected in discrete polar fractions from separations of the complex metabolite mixture by high-pressure liquid chromatography. Their ability to bind to DNA decreased with time at ambient temperature, and they were rapidly deactivated by acid. 7,8-Benzolflavone acted by suppressing the formation of polar metabolites derived from enzymatic oxidation of the aromatic double bonds. The inhibitor had no effect on the enzymes hydroxylating saturated carbon; hence it is unlikely that metabolism of the methyl group is important in conversion of this carcinogen to its proximate form, although the presence of the 11-methyl group is essential for carcinogenic activity in this series. (+info)The repressed nuclear receptor CAR responds to phenobarbital in activating the human CYP2B6 gene. (2/3060)
The endogenous CYP2B6 gene becomes phenobarbital (PB) inducible in androstenol-treated HepG2 cells either transiently or stably transfected with a nuclear receptor CAR expression vector. The PB induction mediated by CAR is regulated by a conserved 51-base pair element called PB-responsive enhancer module (PBREM) that has now been located between -1733 and -1683 bp in the gene's 5'-flanking region. An in vitro translated CAR acting as a retinoid X receptor alpha heterodimer binds directly to the two nuclear receptor sites NR1 and NR2 within PBREM. In a stably transfected HepG2 cell line, both PBREM and NR1 are activated by PB and PB-type compounds such as chlorinated pesticides, polychlorinated biphenyls and chlorpromazine. In addition to PBREM, CAR also transactivates the steroid/rifampicin-response element of the human CYP3A4 gene in HepG2 cells. Thus, activation of the repressed nuclear receptor CAR appears to be a versatile mediator that regulates PB induction of the CYP2B and other genes. (+info)Induction of hepatic cytochromes P450 in dogs exposed to a chronic low dose of polychlorinated biphenyls. (3/3060)
Induction of cytochrome P450 isoforms, specifically CYP1A1, and their catalytic activities are potential biomarkers of environmental contamination by polychlorinated biphenyls (PCBs). In this study, dogs were exposed to 25 ppm or 5 ppm Aroclor 1248 (PCB mixture) daily in their diet for 10 or 20 weeks, respectively. Relative to controls, hepatic microsomes from dogs dosed with PCBs had higher levels of CYP1A1 detected in immunoblots and higher levels of EROD activity, but low levels of induction for CYP2B and PROD activity. Concentrations of 96 PCB congeners in serum and liver were evaluated using capillary chromatography. Results showed that all dogs exposed to PCB mixtures had higher levels of PCB in serum and liver. Dogs preferentially sequestered highly chlorinated PCB congeners in liver relative to serum. With these experiments, we demonstrated that EROD activity was a potentially sensitive marker of PCB exposure at 5 and 25 ppm. Furthermore, CYP1A1 and EROD activity were maximally induced in dogs consuming dietary concentrations only 2.5 times the maximal permissible level for human food (FDA). The value of CYP1A1 induction as a biomarker of PCB exposure was tenuous because neither CYP1A1 levels nor EROD activity correlated with total PCB body burden. However, a small subset of congeners were identified in liver that may strongly influence EROD and PROD induction. Finally, two dogs in the 25 ppm dose group were fasted for 48 h. After 24 h of fasting, several new congeners appeared in the serum and remained in the serum for the remainder of the fast. The fast caused a 293% increase in PCB concentration in serum. This increase has strong implications regarding mobilization of toxic PCBs in wildlife during fasting (e.g., migration, hibernation). (+info)Regulation of cytochrome P-450 (CYP) 1B1 in mouse Hepa-1 variant cell lines: A possible role for aryl hydrocarbon receptor nuclear translocator (ARNT) as a suppressor of CYP1B1 gene expression. (4/3060)
Cytochrome P-450 (CYP) 1B1 expression in mouse hepatoma (Hepa-1) wild-type (WT) cells was compared with responses in Hepa-1 variants LA1 and LA2, which, respectively, exhibit low aryl hydrocarbon receptor (AhR) level and defective AhR nuclear translocator (ARNT) protein. 10T1/2 mouse embryo fibroblasts express predominantly CYP1B1 and at a 100 times higher level than in Hepa-1 cells, whereas they express about 300-fold lower CYP1A1 than Hepa-1 cells. The expression of CYP1B1 in WT and LA1 variant, although at a much lower level, follows that of CYP1A1, reflecting their common regulation through the AhR. The LA2 (ARNT-defective) cells showed a major difference between CYP1B1 and CYP1A1 expression. Although CYP1A1 mRNA levels in LA2 were extremely low and unresponsive to 2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD), basal CYP1B1 mRNA and protein were expressed at levels similar to those seen in TCDD-induced WT. The elevated basal CYP1B1 mRNA in LA2 cells decreased by 50% after transient transfection of ARNT cDNA, in parallel with substantial restoration of CYP1A1 induction. This implicates ARNT as a suppressor of CYP1B1 basal expression in Hepa cells. In transient CYP1B1-luciferase constructs in LA2 cells, ARNT shows stimulatory effects in the enhancer region but an inhibitory effect on the proximal promoter. Two CYP1B1 enhancer elements [xenobiotic-responsive element (XRE) 1/2 and XRE4] formed TCDD-unresponsive complexes of similar mobility to TCDD-stimulated AhR-ARNT complex with XRE5. However, because these two complexes were formed to the same extent in LA2 as in WT cells, they cannot be due to ARNT or contribute to ARNT-regulated suppression. (+info)Cytochrome P450 CYP1B1 determines susceptibility to 7, 12-dimethylbenz[a]anthracene-induced lymphomas. (5/3060)
CYP1B1-null mice, created by targeted gene disruption in embryonic stem cells, were born at the expected frequency from heterozygous matings with no observable phenotype, thus establishing that CYP1B1 is not required for mouse development. CYP1B1 was not detectable in cultured embryonic fibroblast (EF) or in different tissues, such as lung, of the CYP1B1-null mouse treated with the aryl hydrocarbon receptor agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin whereas the equivalent wild-type EF cells express basal and substantial inducible CYP1B1 and lung expresses inducible CYP1B1. CYP1A1 is induced to far higher levels than CYP1B1 in liver, kidney, and lung in wild-type mice and is induced to a similar extent in CYP1B1-null mice. 7,12-dimethylbenz[a]anthracene (DMBA) was toxic in wild-type EFs that express CYP1B1 but not CYP1A1. These cells effectively metabolized DMBA, consistent with CYP1B1 involvement in producing the procarcinogenic 3,4-dihydrodiol as a major metabolite, whereas CYP1B1-null EF showed no significant metabolism and were resistant to DMBA-mediated toxicity. When wild-type mice were administered high levels of DMBA intragastrically, 70% developed highly malignant lymphomas whereas only 7.5% of CYP1B1-null mice had lymphomas. Skin hyperplasia and tumors were also more frequent in wild-type mice. These results establish that CYP1B1, located exclusively at extrahepatic sites, mediates the carcinogenicity of DMBA. Surprisingly, CYP1A1, which has a high rate of DMBA metabolism in vitro, is not sufficient for this carcinogenesis, which demonstrates the importance of extrahepatic P450s in determining susceptibility to chemical carcinogens and validates the search for associations between P450 expression and cancer risk in humans. (+info)Effect of cryopreservation on cytochrome P-450 enzyme induction in cultured rat hepatocytes. (6/3060)
In the present study, we evaluated the inducibility of cytochrome P-450 (CYP) CYP1A, CYP2B, CYP3A, and CYP4A by beta-naphthoflavone, phenobarbital, dexamethasone, and clofibric acid, respectively, in primary hepatocyte cultures prepared from both fresh and cryopreserved rat hepatocytes. Rat hepatocytes were successfully thawed and cultured after cryopreservation in liquid nitrogen for up to 1 month. Percentage of total recovery, viable cell recovery, and final viability of the cells were 68%, 72%, and 85%, respectively. Regardless of whether they were cryopreserved or not, cultured hepatocytes exhibited near-normal morphology. Treatment of cryopreserved hepatocytes with beta-naphthoflavone caused an 8-fold increase in 7-ethoxyresorufin O-dealkylase (CYP1A1/2) activity, with an EC50 of 1.5 microM; treatment with phenobarbital caused a 26-fold increase in 7-pentoxyresorufin O-dealkylase (CYP2B1/2) activity, with an EC50 of 10 microM; treatment with dexamethasone caused a 10-fold increase in testosterone 6beta-hydroxylase (CYP3A1/2) activity, with an EC50 of 1.3 microM, whereas treatment with clofibric acid caused a 3-fold increase in lauric acid 12-hydroxylase (CYP4A1-3) activity, with an EC50 of 170 microM. The induction of CYP1A, CYP2B, CYP3A, and CYP4A enzymes by these inducers was confirmed by Western immunoblotting. The patterns of P-450 induction in cryopreserved rat hepatocytes, in terms of concentration response, reproducibility, magnitude, and specificity of response, were similar to those observed in freshly isolated hepatocytes. Additionally, the magnitude and specificity of induction was similar to that observed in vivo in rats. In conclusion, under the conditions examined, cryopreserved rat hepatocytes appear to be a suitable in vitro system for evaluating xenobiotics as inducers of P-450 enzymes. (+info)The aromatase inactivator 4-hydroxyandrostenedione (4-OH-A) inhibits tamoxifen metabolism by rat hepatic cytochrome P-450 3A: potential for drug-drug interaction of tamoxifen and 4-OH-A in combined anti-breast cancer therapy. (7/3060)
Tamoxifen (tam), an anti-breast cancer agent, is metabolized into tam-N-oxide by the hepatic flavin-containing monooxygenase and into N-desmethyl- and 4-hydroxy-tam by cytochrome P-450s (CYPs). Additionally, tam is metabolically activated by hepatic CYP3A, forming a reactive intermediate that binds covalently to proteins. Tam and 4-hydroxyandrostenedione (4-OH-A) are currently used to treat breast cancer, and it has been contemplated that 4-OH-A be given concurrently with tam to contravene potential tumor resistance to tam. Because alterations in tam metabolism may influence its therapeutic efficacy, the effect of 4-OH-A on tam metabolism was examined. Incubation of tam with liver microsomes from phenobarbital-treated rats, in the presence of 4-OH-A (10-100 microM), resulted in marked inhibition of tam-N-demethylation and tam covalent binding and in decreased tam-N-oxide accumulation; however, there was no inhibition of the formation of 4-hydroxy-tam and of 3,4-dihydroxytamoxifen. These findings indicate that 4-OH-A inhibits CYP3A, but not P-450(s) that catalyze tam 4-hydroxylation. The diminished tam-N-oxide accumulation could be due to decreased N-oxide formation and/or due to increased N-oxide reduction. Incubation of tam-N-oxide with liver microsomes containing heat-inactivated flavin-containing monooxygenase demonstrated that 4-OH-A increases the accumulation of tam, possibly by diminishing its P-450-mediated metabolism. Kinetic studies indicate that 4-OH-A is a competitive inhibitor of CYP3A, but not a time-dependent inactivator. Consequently, the concurrent treatment of tam and 4-OH-A may result in increased tam half-life and thus could potentiate the therapeutic efficacy of tam and diminish the potential side effects of tam by inhibiting its covalent binding to proteins and possibly to DNA. (+info)Quantitative analysis of constitutive and 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced cytochrome P450 1B1 expression in human lymphocytes. (8/3060)
Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD or dioxin) results in a broad spectrum of biological responses, including altered metabolism, disruption of normal hormone signaling pathways, reproductive and developmental effects, and cancer. Cytochrome P450 1B1 (CYP1B1) is a dioxin-inducible gene that is active in the formation of 4-hydroxyestradiol, a potentially genotoxic catechol estrogen. Therefore, the analysis of CYP1B1 in humans may be useful in establishing relationships between dioxin exposure and adverse health effects. In this study, we examined the expression of CYP1B1 in human peripheral blood lymphocytes of unexposed individuals using a quantitative reverse transcription-PCR method. Absolute CYP1B1 RNA levels varied more than 30-fold in uncultured mononuclear cells obtained from 10 individuals. In vitro treatment of mitogen-stimulated lymphocytes with TCDD for 1-5 days of culture resulted in a peak induction of CYP1B1 after 3 days. The induction of CYP1B1 RNA levels after 3 days of culture was dose-dependent, exhibited a maximum response above 10 nM TCDD, and varied greatly among different individuals. However, the half maximal dose required for this induction was similar between individuals and comparable to that observed in the MCF-7 and HepG2 human cell lines. These observations indicate that CYP1B1 exhibits variable constitutive expression and is inducible in vitro by TCDD in human lymphocytes and that the magnitude of induction varies within the population. These data define the suitability of CYP1B1 for use as a mechanistically based biomarker in ongoing molecular epidemiological studies of human populations exposed to dioxins and related chemicals that bind the aromatic hydrocarbon receptor. (+info)
Distribution of aryl hydrocarbon hydroxylase inducibility in cultured by B Paigen, J Minowada et al.
Plus it
Potent mechanism-based sirtuin-2-selective inhibition by an in situ -generated occupant of the substrate-binding site, ...
CYP2A6 - SNPedia
Identification and partial characterization of a novel CYP2C9 splicing variant encoding a protein lacking eight amino acid...
Aryl hydrocarbon hydroxylase in persons with lung or laryngeal cancer by E Ward, B Paigen et al.
Other - Selective Inhibitors of HDAC signaling pathway
I found out recently that I am a poor/intermediate metabolizer of CYP2D6 and have been been confusing myself ever since
Lack of an effect of a novel beta3-adrenoceptor agonist, TAK-677, on energy metabolism in obese individuals: a double-blind,...
Hepatitis C[Majr] AND Complementary Therapies[Majr] AND humans[MeS - PubMed - NCBI
Metabolic activation of aromatic hydrocarbons in purified rat liver nuclei: induction of enzyme activities and binding to DNA...
Cyp1b1-Related Primary Congenital Glaucoma disease: Malacards - Research Articles, Drugs, Genes, Clinical Trials
Plus it
Recombinant Human CYP11A1 Protein, Myc/DDK-tagged, C13 and N15-labeled CYP11A1-4529H - Creative BioMart
c1 (B6NLxv1c2) ATCC ® CRL-2716™ Mus musculus liver hepatoma
Do mammalian cytochrome P450s show multiple ligand access pathways and ligand channelling? | EMBO Reports
Identification and developmental expression of the full complement of Cytochrome P450 genes in Zebrafish
Stable expression of human cytochrome P4502E1 in HepG2 cells:...
Trichloroepoxypropane
Summary Report | CureHunter
P450 - McArthur Lab
Pages that link to Primary Congenital Glaucoma - EyeWiki
Nicotine Metabolism (Homo sapiens) - WikiPathways
anti-CYP2E1 antibody [9E6] | GeneTex
anti-CYP46A1 antibody [N1C2] | GeneTex
Anti-CYP3A13 Antibody Products | Biocompare
Anti-CYP2B1 Antibody Products | Biocompare
Anti-CYP1A1 Antibody (1A3-03) | 圣克鲁斯生物技术
Do I Really Live in This Sitcom?: I Nearly Died! (well maybe not)
Anti-CYP27B1 抗体 (ab95047) | アブカム
cornavita: drug-roulette - cornavitas Webseite!
Studying | 4GMF
AUCNY Manhasset - 535 Plandome Rd. Manhasset, NY 11030 | 516-627-6188
Polyclonal antibody for human cytochrome P450 enzyme CYP3A4 - Quicktech
Plus it
Identification of Novel Cytochrome P450s in the Acari - Northumbria Research Link
VALIDATED ASSAYS FOR HUMAN CYTOCHROME P450 ACTIVITIES | Drug Metabolism & Disposition
Plus it
OPUS Würzburg | Search
Human Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2) Protein, Recombinant | ABIN6303723
Clinical Trials Registry | Internet Stroke Center
Biochem Ultimate Fat Metabolizer 60 Tabs - Swanson Health Products
Order Gloryfeel Metabolizer Thermostat | Gloryfeel Metabolizer Thermo | Generic Pharmacy Online
CypExpress™ - A Human Cytochrome P450 Catalytic System | Sigma-Aldrich
anti-CYP26A1 Antikörper (C-Term) (Biotin) | Produkt Nr. ABIN4266057
Other highlights in the April 29 JNCI ( Selecting Patients Based on Genotyp...)
Properties of Warfarin Essay - 376 Words
OriGene - CYP51A1 (NM 000786) Human Protein
Mansuy, D.
DSpace at EWHA: Polymorphisms of vitamin K-related genes (EPHX1 and VKORC1L1) and stable warfarin doses
Selective deficiency of debrisoquine 4-hydroxylase activity in mouse liver microsomes<...
Polyclonal antibody for human cytochrome P450 enzyme CYP2D6 - Quicktech
FELDENE® (piroxicam) Clinical Pharmacology | Pfizer Información Médica - República de Guatemala (Republic of Guatemala)
Determinants of Warfarin Metabolism - Full Text View - ClinicalTrials.gov
What Is Primary Congenital Glaucoma? Hereditary, Symptoms & Treatment
Recombinant Human Constitutive androstane receptor protein (ab81846) Protocols
Quantitative Prediction of Human Pregnane X Receptor and Cytochrome P450 3A4 Mediated Drug-Drug Interaction in a Novel Multiple...
AID 769674 - Drug metabolism in human liver microsomes assessed as CYP3A4-mediated metabolite formation in presence of...
AID 510137 - Ratio of IC50 for CYP3A4 in human liver microsomes measured immediately to IC50 for CYP3A4 in human liver...
Evaluation of metabolism dependent inhibition of CYP2B6 mediated bupropion hydroxylation in human liver microsomes by monoamine...
Pharmaceuticals | Free Full-Text | Role of CYP2C9, CYP2C19 and EPHX Polymorphism in the Pharmacokinetic of Phenytoin: A Study...
Smokers Nicotine Metabolism Increased by Drugs for Neurologic, Psychiatric Conditions
Isoniazid is a mechanism-based inhibitor of cytochrome P450 1A2, 2A6, 2C19 and 3A4 isoforms in human liver microsomes.
Kinetic and Mechanistic Studies on Cytochrome P450-Related High-Valent Porphyrin-Iron-Oxo Species by Zhengzheng Pan |...
Plus it
Effects of NR1I2 TGT and CYP2B6*6 on induction of bupro | Open-i
Cardiac Pharmacology - Textbook of Cardiology
Cardiac Pharmacology - Textbook of Cardiology
Warfarin Metabolism - LabCE.com, Laboratory Continuing Education
Экспрессия генов цитохромов р450 опухоли у больных с раком молочной железы
CYP2C9 Genotype-dependent Warfarin Pharmacokinetics: Impact of CYP2C9 Genotype on R- and S-Warfarin and Their Oxidative...
anti-CYP4A22 Primary Antibodies
The interindividual variability in metabolism of the analgesic tramadol in neonates is independent of their disease state...
Cyp2e1 MGI Mouse Gene Detail - MGI:88607 - cytochrome P450, family 2, subfamily e, polypeptide 1
Cyp4f14 MGI Mouse Gene Detail - MGI:1927669 - cytochrome P450, family 4, subfamily f, polypeptide 14
Produktübersicht anti-CYP4F22 Antikörper
Antibodypedia - TA503437 - OriGene CYP17A1 antibody
文章详细信息
Rythmol SR Drug Interactions
CYP2D6-ГЕНОТИПИРОВАНИЕ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ ТЕРАПИИ ТАМОКСИФЕНОМ У БОЛЬНЫХ ГОРМОНОПОЗИТИВНЫМ РАКОМ МОЛОЧНОЙ ЖЕЛЕЗЫ | Любченко...
CiNii 論文 -
遺伝子多型情報に基づく投与指針作成に向けて : CYP2C19
CYP1A1
... is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons ... Kiyohara C, Hirohata T, Inutsuka S (Jan 1996). "The relationship between aryl hydrocarbon hydroxylase and polymorphisms of the ... and the aryl hydrocarbon receptor nuclear translocator. In the intestine, but not the liver, CYP1A1 expression moreover depends ... is regulated by a heterodimeric transcription factor that consist of the aryl hydrocarbon receptor, a ligand activated ...
Cunninghamella
Aryl hydrocarbon hydroxylase activity in the fungus Cunninghamella bainieri: Evidence for the presence of cytochrome P-450. J.P ... Cerniglia, Carl E. (1992). "Biodegradation of polycyclic aromatic hydrocarbons". Biodegradation. 3 (2-3): 351-368. doi:10.1007/ ... Many species are also capable of oxidizing polycyclic aromatic hydrocarbons, a class of stable organic molecules that tends to ...
EPHX1
... basal activities and inducibility of epoxide hydrolases and aryl hydrocarbon hydroxylase". Biochem. Pharmacol. 33 (1): 71-7. ... Casson AG, Zheng Z, Porter GA, Guernsey DL (2006). "Genetic polymorphisms of microsomal epoxide hydroxylase and glutathione S- ... but more complex compounds as polycyclic aromatic hydrocarbons are rather bioactivated to genotoxic species. EPHX1 mediates the ... "Stereoselective epoxidation and hydration at the K-region of polycyclic aromatic hydrocarbons by cDNA-expressed cytochromes ...
O-Toluidine
Exposure to o-toluidine enhances the microsomal activity of aryl hydrocarbon hydroxylase (particularly in the kidney), NADPH- ...
Unspecific monooxygenase
... aryl-4-monooxygenase, aryl hydrocarbon hydroxylase, microsomal P-450, flavoprotein-linked monooxygenase, and flavoprotein ... Mitoma C, Udenfriend S (1962). "Aryl-4-hydroxylase". Methods Enzymol. 5: 816-819. doi:10.1016/s0076-6879(62)05318-5. Napoli JL ... Nebert DW, Gelboin HV (1968). "Substrate-inducible microsomal aryl hydroxylase in mammalian cell culture. I. Assay and ...
List of MeSH codes (D12.776)
... aryl hydrocarbon hydroxylases MeSH D12.776.422.220.453.040.050 - aniline hydroxylase MeSH D12.776.422.220.453.040.110 - ... steroid 11-beta-hydroxylase MeSH D12.776.422.220.453.915.730 - steroid 12-alpha-hydroxylase MeSH D12.776.422.220.453.915.737 - ... steroid 16-alpha-hydroxylase MeSH D12.776.422.220.453.915.748 - steroid 17-alpha-hydroxylase MeSH D12.776.422.220.453.915.760 ... cholesterol 7 alpha-hydroxylase MeSH D12.776.422.220.453.915.212 - cholesterol side-chain cleavage enzyme MeSH D12.776.422.220. ...
List of MeSH codes (D08)
... aryl hydrocarbon hydroxylases MeSH D08.244.453.040.050 - aniline hydroxylase MeSH D08.244.453.040.110 - benzopyrene hydroxylase ... aryl hydrocarbon hydroxylases MeSH D08.811.682.690.708.170.040.024 - 7-alkoxycoumarin o-dealkylase MeSH D08.811.682.690.708.170 ... steroid 11-beta-hydroxylase MeSH D08.244.453.915.730 - steroid 12-alpha-hydroxylase MeSH D08.244.453.915.737 - steroid 16-alpha ... hydroxylase MeSH D08.244.453.915.748 - steroid 17-alpha-hydroxylase MeSH D08.244.453.915.760 - steroid 21-hydroxylase MeSH ...
Methylcholanthrene
1990) compared the effects of fetal versus adult exposure to 3-MC on both induction of aryl hydrocarbon hydroxylase (AHH) ... 3-MC is a ligand of the aryl hydrocarbon receptor (AhR), which stimulates transcription directed by xenobiotic response ... Shipley, J. M.; Waxman, D.J. (2006). "Aryl hydrocarbon receptor-independent activation of estrogen receptor-dependent ... This is likely because it is plausible that two polycyclic aromatic hydrocarbons are metabolized via the same pathway. ...
Kynurenine
Nguyen NT, Kimura A, Nakahama T, Chinen I, Masuda K, Nohara K, Fujii-Kuriyama Y, Kishimoto T (2010). "Aryl hydrocarbon receptor ... Kynurenine 3-hydroxylase converts kynurenine to 3-hydroxykynurenine. Kynurenine has also been identified as one of two ... aryl hydrocarbon receptor and plasma kynurenine in major depressive disorder: metabolomics-informed genomics". Translational ... "An endogenous tumour-promoting ligand of the human aryl hydrocarbon receptor". Nature. 478 (7368): 197-203. Bibcode:2011Natur. ...
Benzoyl-CoA
This enzyme is responsible in part for the reductive dearomatization of aryl compounds mediated by bacteria under anaerobic ... This intermediate is subsequently converted by a benzophenone 3′-hydroxylase, a cytochrome P450 monooxygenase, leading to the ... "Anaerobic oxidation of aromatic compounds and hydrocarbons" Current Opinion in Chemical Biology 2002 Volume 6, pp. 604-611. doi ...
Decarboxylation
... of aryl carboxylates can generate the equivalent of the corresponding aryl anion, which in turn can undergo ... Iron-based hydroxylases operate by reductive activation of O2 using the decarboxylation of alpha-ketoglutarate as an electron ... Hydrodecarboxylations involve the conversion of a carboxylic acid to the corresponding hydrocarbon. This is conceptually the ...
Hypoxia-inducible factor
... the latter being a constitutively-expressed aryl hydrocarbon receptor nuclear translocator (ARNT). HIF-1 belongs to the PER- ... By inhibiting prolyl-hydroxylase enzyme, the stability of HIF-2α in the kidney is increased, which results in an increase in ... factor 1-alpha PDBe-KB provides an overview of all the structure information available in the PDB for Human Aryl hydrocarbon ... In normal circumstances after injury HIF-1a is degraded by prolyl hydroxylases (PHDs). In June 2015, scientists found that the ...
HIF1A
... and the aryl hydrocarbon receptor nuclear translocator (Arnt), the beta subunit. HIF1A contains a basic helix-loop-helix domain ... In normal circumstances after injury HIF1A is degraded by prolyl hydroxylases (PHDs). In June 2015, scientists found that the ... In addition, the coordinated activity of the prolyl hydroxylases (PHDs) maintain the appropriate balance of HIF1A protein in ... Bruick RK, McKnight SL (November 2001). "A conserved family of prolyl-4-hydroxylases that modify HIF". Science. 294 (5545): ...
Angelicin
... is due to the fact that angelicin decreases the activity and expression of CYP1A1 which is regulated by aryl hydrocarbon ... 4-Coumaric acid 2-hydroxylase (C2'H) hydroxylates the p-coumaric acid at the ortho position. Notably, this reaction uses alpha- ... Enzymes such as ammonialyases, methylases and hydroxylases then transform these amino acids to cinnamic acid derivatives which ...
Cunninghamella elegans
A. Jaworski; L. Sedlaczek; J. Dlugoński; Ewa Zajaczkowska (1985). "Inducible nature of the steroid 11-hydroxylases in spores of ... Cytochrome P450 monooxygenase, aryl sulfotransferase, glutathione S-transferase, UDP-glucuronosyltransferase, UDP- ... elegans has been implicated in the neutralization of numerous polycyclic aromatic hydrocarbons (PAH). It can degrade molecules ...
Cytochrome P450
... steroid 20α-hydroxylase, steroid 22-hydroxylase, cholesterol side-chain scission). CYP11B1 (encoding the protein P450c11β) ... are inducible by some polycyclic hydrocarbons, some of which are found in cigarette smoke and charred food. These enzymes are ... phenacetin Heterocyclic aryl amines Inducible and CYP1A2 5-10% deficient oxidize uroporphyrinogen to uroporphyrin (CYP1A2) in ... found in the inner mitochondrial membrane of adrenal cortex has steroid 11β-hydroxylase, steroid 18-hydroxylase, and steroid 18 ...
Cytochrome P450
retinoic acid hydroxylase. 3 subfamilies, 3 genes. CYP26A1, CYP26B1, CYP26C1 CYP27. varied. 3 subfamilies, 3 genes. CYP27A1 ( ... Oxygen rebound mechanism utilized by cytochrome P450 for conversion of hydrocarbons to alcohols via the action of "compound I ... Clozapine, imipramine, paracetamol, phenacetin Heterocyclic aryl amines Inducible and CYP1A2 5-10% deficient oxidize ... cholesterol 24-hydroxylase. 1 subfamily, 1 gene. CYP46A1 CYP51. cholesterol biosynthesis. 1 subfamily, 1 gene, 3 pseudogenes. ...
Phenols
... uses an aryl dialkyl phosphate and H2O to produce dialkyl phosphate and an aryl alcohol. ... Shih, C. -H.; Chu, I. K.; Yip, W. K.; Lo, C. (2006). "Differential Expression of Two Flavonoid 3'-Hydroxylase cDNAs Involved in ... bonded directly to an aromatic hydrocarbon group. The simplest of the class is phenol, which is also called carbolic acid C. 6H ... Aryl-alcohol dehydrogenase (NADP+) uses an aromatic alcohol and NADP+ to produce an aromatic aldehyde, NADPH and H+. ...
Induction of Aryl Hydrocarbon Hydroxylase in Ambystoma tigrinum - Digital Library
The half life of enzyme activity induced in vivo was related to the excretion of hydrocarbon metabolites. ... or 10-fold by addition of aromatic polycyclic hydrocarbons to the aqueous environment of the neotene animal. The cytochrome P- ... Aryl hydrocarbon hydroxylase (AHH) was induced 15-fold in Ambystoma tigrinum by intraperitoneal injection of 3- ... Colvin, David P. Induction of Aryl Hydrocarbon Hydroxylase in Ambystoma tigrinum, thesis, December 1974; Denton, Texas. ( ...
Aryl hydrocarbon hydroxylase in asbestos workers. | Harvard Catalyst Profiles | Harvard Catalyst
Genetic Expression of Aryl Hydrocarbon Hydroxylase Induction | Molecular Pharmacology
Induction of both diphenylhydantoin hydroxylase and aryl hydrocarbon hydroxylase occurs in the liver of 3-methylcholanthrene- ... is associated with aryl hydrocarbon (benzo[a]pyrene) hydroxylase induction among inbred strains of aromatic hydrocarbon-treated ... is associated with the presence of aryl hydrocarbon hydroxylase induction among aromatic hydrocarbon-treated inbred C57BL/6N, ... Genetic Expression of Aryl Hydrocarbon Hydroxylase Induction Message Subject (Your Name) has forwarded a page to you from ...
Genetic Expression of Aryl Hydrocarbon Hydroxylase Induction | Molecular Pharmacology
Aryl hydrocarbon hydroxylase induction by polycyclic hydrocarbons is expressed as a simple autosomal dominant trait; in any ... Genetic Expression of Aryl Hydrocarbon Hydroxylase Induction. III. Changes in the Binding of n-Octylamine to Cytochrome P-450. ... Genetic Expression of Aryl Hydrocarbon Hydroxylase Induction. D. W. NEBERT, J. E. GIELEN and F. M. GOUJON ... Genetic Expression of Aryl Hydrocarbon Hydroxylase Induction. D. W. NEBERT, J. E. GIELEN and F. M. GOUJON ...
Aryl Hydrocarbon Hydroxylase Represents CYP1B1, and not CYP1A1, in Human Freshly Isolated White Cells | Cancer Epidemiology,...
Lieberman J. Aryl hydrocarbon hydroxylase in bronchogenic carcinoma. N. Engl. J. Med., 298: 686-687, 1978. ... Aryl Hydrocarbon Hydroxylase Represents CYP1B1, and not CYP1A1, in Human Freshly Isolated White Cells. Trimodal Distribution of ... Ward E., Paigen B., Steenland K., Vincent R., Minowada J., Gurtoo H. L., Sartori P., Havens M. B. Aryl hydrocarbon hydroxylase ... Kärki N. T., Pokela R., Nuutinen L., Pelkonen O. Aryl hydrocarbon hydroxylase in lymphocytes and lung tissue from lung cancer ...
The relationship between CYP1A1 aryl hydrocarbon hydroxylase activity and lung cancer in a Japanese population<...
N2 - Because aryl hydrocarbon hydroxylase (AHH) is considered to be responsible for the activation of benzo(a)pyrene and other ... AB - Because aryl hydrocarbon hydroxylase (AHH) is considered to be responsible for the activation of benzo(a)pyrene and other ... Because aryl hydrocarbon hydroxylase (AHH) is considered to be responsible for the activation of benzo(a)pyrene and other ... abstract = "Because aryl hydrocarbon hydroxylase (AHH) is considered to be responsible for the activation of benzo(a)pyrene and ...
Aryl hydrocarbon hydroxylase activity levels in the hepatic microsomal fraction from MC- or TCDD-treated mice: a comparison...
The effects of in vivo administration of polycyclic aromatic hydrocarbons on the levels of aryl hydrocarbon hydroxylase (AHH) ... N2 - The effects of in vivo administration of polycyclic aromatic hydrocarbons on the levels of aryl hydrocarbon hydroxylase ( ... AB - The effects of in vivo administration of polycyclic aromatic hydrocarbons on the levels of aryl hydrocarbon hydroxylase ( ... "The effects of in vivo administration of polycyclic aromatic hydrocarbons on the levels of aryl hydrocarbon hydroxylase (AHH) ...
"A COMPARISON OF ARYL HYDROCARBON HYDROXYLASE INDUCTION PHENOTYPES AMON" by CYNTHIA JANE. FORSTER-GIBSON
Aryl hydrocarbon hydroxylase (AHH) activity in two cell lines - Hepa-1c1 and H4IIE-C3 was induced to similar levels by benzo(a ... Aryl hydrocarbon hydroxylase (AHH) activity in two cell lines - Hepa-1c1 and H4IIE-C3 was induced to similar levels by benzo(a ... A COMPARISON OF ARYL HYDROCARBON HYDROXYLASE INDUCTION PHENOTYPES AMONG AND WITHIN CELL LINES. ... FORSTER-GIBSON, CYNTHIA JANE., "A COMPARISON OF ARYL HYDROCARBON HYDROXYLASE INDUCTION PHENOTYPES AMONG AND WITHIN CELL LINES ...
Part 2 Chapter 9 Tables|HuGE|Books|Resources|Genomics|CDC
Distribution of aryl hydrocarbon hydroxylase inducibility in cultured" by B Paigen, J Minowada et al.
We measured aryl hydrocarbon hydroxylase (AHH) in cultured human lymphocytes. A striking seasonal variation in AHH activity was ... We measured aryl hydrocarbon hydroxylase (AHH) in cultured human lymphocytes. A striking seasonal variation in AHH activity was ... Distribution of aryl hydrocarbon hydroxylase inducibility in cultured human lymphocytes." (1977). Faculty Research 1970 - 1979 ...
CYP2B6 and bupropion's smoking-cessation pharmacology: the role of hydroxybupropion
"Genetic regulation of aryl hydrocarbon hydroxylase induction. V. Spec" by D W. Nebert and H Kon
CYP1B1 gene: MedlinePlus Genetics
Aryl hydrocarbon (benzo[a]pyrene) hydroxylases in liver from rats of different age, sex and nutritional status. Distinction of...
... hydroxylases in liver from rats of different age, sex and nutritional status. Distinction of two types by 7,8-benzoflavone. by ... Aryl hydrocarbon (benzo[a]pyrene) hydroxylases in liver from rats of different age, sex and nutritional status. Distinction of ... article{Wiebel1975ArylH, title={Aryl hydrocarbon (benzo[a]pyrene) hydroxylases in liver from rats of different age, sex and ...
Search Results - - 396 Results - Digital Library
Identification of cytochrome P450 3A1/2 as the major P450 isoform responsible for the metabolism of fentanyl by rat liver...
Front Matter | Scientific Frontiers in Developmental Toxicology and Risk Assessment | The National Academies Press
CYP1A1 cytochrome P450 family 1 subfamily A member 1 [Homo sapiens (human)] - Gene - NCBI
aryl hydrocarbon hydroxylase. cytochrome P1-450, dioxin-inducible. cytochrome P450 form 6. cytochrome P450, family 1, subfamily ... involved_in omega-hydroxylase P450 pathway TAS Traceable Author Statement. more info ... enables estrogen 2-hydroxylase activity IDA Inferred from Direct Assay. more info ... enables estrogen 16-alpha-hydroxylase activity IDA Inferred from Direct Assay. more info ...
Aberrant CYP1A1 Induction: Discrepancy of CYP1A1 mRNA and Aryl Hydrocarbon Hydroxylase Activity in Mutant Cells of Mouse...
T2 - Discrepancy of CYP1A1 mRNA and Aryl Hydrocarbon Hydroxylase Activity in Mutant Cells of Mouse Hepatoma Line, Hepa‐1 ... Aberrant CYP1A1 Induction: Discrepancy of CYP1A1 mRNA and Aryl Hydrocarbon Hydroxylase Activity in Mutant Cells of Mouse ... Aberrant CYP1A1 Induction : Discrepancy of CYP1A1 mRNA and Aryl Hydrocarbon Hydroxylase Activity in Mutant Cells of Mouse ... Aberrant CYP1A1 Induction : Discrepancy of CYP1A1 mRNA and Aryl Hydrocarbon Hydroxylase Activity in Mutant Cells of Mouse ...
Inhibitory effects of snuff extract on ornithine decarboxylase and aryl hydrocarbon hydroxylase activities in relation to cell...
Animals , Aryl Hydrocarbon Hydroxylases/antagonists & inhibitors , Carcinogens , Cell Division , Cells, Cultured , Mouth ... Inhibitory effects of snuff extract on ornithine decarboxylase and aryl hydrocarbon hydroxylase activities in relation to cell ... and aryl hydrocarbon hydroxylase (AHH) was studied in primary embryonal mouse tongue cultures. Cultures treated with SE in ... Aryl Hydrocarbon Hydroxylases / Cell Division / Cells, Cultured / Tobacco, Smokeless / Animals Language: English Journal: ...
Oxidative biotransformation of 2-acetylaminofluorene in fetal and placental tissues of humans and monkeys. Correlations with...
Catechol estrogen formation in the mouse uterus and its role in implantation.
Estradiol-2/4-hydroxylase (E-2/4-H) activity was determined in the mouse uterus during early pregnancy as well as in ovarian ... Steroid Hydroxylases; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/Cytochrome P-450 CYP1A1; EC 1.14.14.1/cytochrome ... Aryl Hydrocarbon Hydroxylases*. Chromatography, High Pressure Liquid. Cytochrome P-450 CYP1A1*. Cytochrome P-450 Enzyme System ... Estradiol-2/4-hydroxylase (E-2/4-H) activity was determined in the mouse uterus during early pregnancy as well as in ovarian ...
CYP2C19 | Cancer Genetics Web
CYP3A5 | Cancer Genetics Web
Cunninghamella - Wikipedia
Aryl hydrocarbon hydroxylase activity in the fungus Cunninghamella bainieri: Evidence for the presence of cytochrome P-450. J.P ... Cerniglia, Carl E. (1992). "Biodegradation of polycyclic aromatic hydrocarbons". Biodegradation. 3 (2-3): 351-368. doi:10.1007/ ... Many species are also capable of oxidizing polycyclic aromatic hydrocarbons, a class of stable organic molecules that tends to ...
000646 - A/J
Ahr, aryl-hydrocarbon receptor. Gene Synonym(s). bHLHe76; aromatic hydrocarbon responsiveness; aryl hydrocarbon hydroxylase; ... The Aryl hydrocarbon receptor mediates tobacco-induced PD-L1 expression and is associated with response to immunotherapy. Nat ... The chemokine CXCL13 in lung cancers associated with environmental polycyclic aromatic hydrocarbons pollution. Elife 4PubMed: ...
000653 - BUB/BnJ
Green tea extract increases cyclophosphamide-induced teratogenesis by modulating the expression of cytochrome P-450 mRNA.
CYP1A1PAHsBenzoLiverMetabolismHepaticInducibility in culturedInductionCarcinogensInduced by aromatic hydrocarbonsCYP1B1Mouse hepatoma lineLymphocytesInhibitorActivityTissuesLigand activatedPathwayExposurePetroleum hydrocarbonsBiodegradationProteinCellsAromatic compoundsGeneticMethylcholanthreneToxicityCholesterolTCDDLigandsInhibition
CYP1A113
- Because aryl hydrocarbon hydroxylase (AHH) is considered to be responsible for the activation of benzo(a)pyrene and other polyaromatic hydrocarbons in cigarette smoke to carcinogens, it is important to examine CYP1A1 (AHH) activity in the determination of susceptibility to lung cancer. (elsevier.com)
- CYP1A1‐dependent aryl hydrocarbon hydroxylase activity is not inducible by 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin or 3‐methylcholanthrene in these two cell lines. (elsevier.com)
- Diseases associated with CYP1A1 include Aryl Hydrocarbon Hydroxylase Inducibility and Ehrlich Tumor Carcinoma . (genecards.org)
- CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). (wikipedia.org)
- The expression of the CYP1A1 gene, along with that of CYP1A2/1B1 genes, is regulated by a heterodimeric transcription factor that consist of the aryl hydrocarbon receptor, a ligand activated transcription factor, and the aryl hydrocarbon receptor nuclear translocator. (wikipedia.org)
- Hepa-1c1c7 has high CYP1A1-dependent aryl hydrocarbon hydroxylase (AHH) activity. (atcc.org)
- The transactivating domain of NFI/CTF was found to act in synergy with the arylhydrocarbon receptor pathway during the induction of CYP1A1 by 2,3,7,8-tetrachloro- p -dibenzodioxin. (asm.org)
- CYP1A1 is a ubiquitous member of the P450 superfamily which is among the products of the aryl hydrocarbon (Ah)-inducible gene battery (reference 50 and references therein). (asm.org)
- CYP1A1 debrisoquine 4-hydroxylase was inhibited by the CYP1A1 inhibitor α-naphthoflavone and the CYP1A1 substrate 7-ethoxyresorufin. (aspetjournals.org)
- Anti-CYP1A1 monoclonal antibody (mAb) 1-7-1 abolished CYP1A1 debrisoquine hydroxylase and anti-CYP2D6 mAb 50-1-3 eradicated CYP2D6 debrisoquine 4-hydroxylase. (aspetjournals.org)
- In the present study, to analyze the mechanism of CYP1A2 induction by phenobarbital, we examined whether there is any strain difference in this phenomenon and in particular, any correlation between the induction of CYP1A2 by phenobarbital and the induction of CYP1A1 by polycyclic aryl hydrocarbons. (aspetjournals.org)
- CYP1A1 is known to metabolize polycyclic aromatic hydrocarbons, which are important constituents of coffee, whereas CYP1A2 is involved in the primary metabolism of caffeine. (nature.com)
- The cytochrome P4501A1 encoded by the gene CYP1A1 is known to metabolize polycyclic aromatic hydrocarbons such as benzo(a)pyrene. (nature.com)
PAHs8
- This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. (nih.gov)
- In addition, a variety of substances such as fuel, water, antifreeze, dust, and various combustion products such as polycyclic aromatic hydrocarbons (PAHs), metals, and metallic oxides accumulate in the oil. (cdc.gov)
- Polycyclic aromatic hydrocarbons (PAHs), carcinogen mainly from cigarette smoking and air pollution, have been proved to be related to the development of COPD [ 10 , 11 ]. (hindawi.com)
- Sediments from these sites exhibited a wide range in concentrations of xenobiotic compounds (e.g. concentrations of polycyclic aromatic hydrocarbons (PAHs) ranged from 20 to 50,000 ng/g dry weight and concentrations of polychlorinated biphenyls (PCBs) ranged from 2 to 1,400 ng/g dry weight), with the sites in Boston Harbor and Raritan Bay the most heavily contaminated. (noaa.gov)
- Polycyclic aromatic hydrocarbons (PAHs) and cigarette smoke induce tumors of the respiratory organs in the pulmonary peripheral region in rats, in the pulmonary hilar region in humans and in the upper respiratory tract in Syrian hamsters (see review by Mohr and Dungworth, 1988). (springer.com)
- Embryos from a population of the teleost fish, Fundulus heteroclitus , inhabiting a clean estuary do not survive when exposed to sediment extract from a site highly contaminated with polycyclic aromatic hydrocarbons (PAHs) while embryos derived from a population inhabiting a PAH polluted estuary are remarkably resistant to the polluted sediment extract. (biomedcentral.com)
- Coho salmon and starry flounder Platichthys stellatus , exposed to 200 ppb of cadmium or lead in seawater at l0°C, were fed a model mixture of polycyclic aromatic hydrocarbons (PAHs) consisting of 2-nethylnaphthalene, 2,6-dimethylnaphthalene, and phenanthrene. (noaa.gov)
- One family of these chemicals that are ubiquitous in the environment is polycyclic aromatic hydrocarbons (PAHs), which are released into the environment as a result of petrogenic and pyrolytic processes. (pubmedcentralcanada.ca)
Benzo7
- Aryl hydrocarbon hydroxylase (AHH) activity in two cell lines - Hepa-1c1 and H4IIE-C3 was induced to similar levels by benzo(a)-anthracence. (uwindsor.ca)
- Aryl hydrocarbon (benzo[a]pyrene) hydroxylases in liver from rats of different age, sex and nutritional status. (semanticscholar.org)
- It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo[a]pyrene (BaP), by transforming it to an epoxide. (wikipedia.org)
- It is highly inducible by the persistent environmental contaminant 2,3,7,8-tetrachloro- p -dibenzodioxin (TCDD) and by planar aromatic hydrocarbons, such as 3-methylcholanthrene or benzo(a)pyrene (BP) ( 24 , 44 , 59 ). (asm.org)
- Benzo[ a ]pyrene is a polycyclic aromatic hydrocarbon (PAH) that can be derived from coal tar. (ornl.gov)
- The present study has been undertaken to examine whether exposure to benzo(a)pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH) compound influences the metabolism of fluoranthene (FLA), another PAH compound. (pubmedcentralcanada.ca)
- I. J. Sadowski, J. A. Wright and L. G. Israels, A permeabilized cell system for studying regulation of aryl hydrocarbon hydroxylase: NADPH as rate limiting factor in benzo(a)pyrene metabolism, Int. J. Biochem. (springer.com)
Liver6
- Induction of both diphenylhydantoin hydroxylase and aryl hydrocarbon hydroxylase occurs in the liver of 3-methylcholanthrene-treated Osborne-Mendel rats. (aspetjournals.org)
- Chinook salmon Oncorhynchus tshawytscha , maintained in seawater at 13°C and fed mixtures of chlorobiphenyls and petroleum hydrocarbons separately and together, were examined for changes in hepatic microsomal AHH activity and for alterations in the morphology of liver, kidney, intestine, gill and skin tissues. (noaa.gov)
- Liver aryl hydrocarbon hydroxylase (AHH) specific activity was low in the young NZB, increased gradually with age, and was higher in the female mice. (worldwidescience.org)
- Alphla-nalphthoflavone also inhibits the aryl hydrocarbon hydroxylase activity in homogenates of induced hamster embryo cells and in liver microsomes from rats previously treated with polycyclic aromatic hydrocarbons, but not in microsomes from control rats. (sciencemag.org)
- Effect of topical application of defined constituents of coal tar on skin and liver aryl hydrocarbon hydroxylase and 7-ethoxycoumarin deethylase activities. (cdc.gov)
- Cytochrome P450 and aryl hydrocarbon hydroxylase (AHH) were present in liver and small intestinal mucosa from normal animals, but were undetectable in colon and rectum. (soton.ac.uk)
Metabolism2
- The present research was directed toward elucidating the effect of xenobiotics which alter the metabolism and toxicity of aromatic hydrocarbons by marine fish, as evinced through biochemical changes and altered cellular morphology. (noaa.gov)
- Part II discusses the bioaccumulation and metabolism of hydrocarbons by marine organisms. (elsevier.com)
Hepatic6
- Induction of hydroxylase activity by 3-methylcholanthrene administration to C57BL/6N mice is associated with an approximately equal conversion of hepatic type b to type a P-450, as measured by n -octylamine binding to the cytochrome. (aspetjournals.org)
- Phenobarbital treatment of AhAh Ahah , or ahah mice similarly induces the hepatic aryl hydrocarbon hydroxylase system about 100% and causes identical increases of about 65% in both type a and b P-450. (aspetjournals.org)
- The effects of in vivo administration of polycyclic aromatic hydrocarbons on the levels of aryl hydrocarbon hydroxylase (AHH) activity in aromatic hydrocarbon (Ah) responsive and non-responsive strains of mice were studied using the hepatic microsomal fraction. (elsevier.com)
- and for possible changes in the activities of hepatic microsomal aryl hydrocarbon hydroxylase (AHH). (noaa.gov)
- In addition, the activities of hepatic AHH were induced by mixtures of the chlorobiphenyls and petroleum hydrocarbons, but not by the chlorobiphenyls alone or hydrocarbons alone. (noaa.gov)
- The differences for the coho and chinook salmon in terms of the responses of the hepatic AHH systems to petroleum hydrocarbons and chlorobiphenyls may have been caused by differences due to seasonal parameters between the two experiments. (noaa.gov)
Inducibility in cultured2
Induction5
- Genetic regulation of aryl hydrocarbon hydroxylase induction. (jax.org)
- Effect of snuff extract (SE) on cell proliferation as measured by 3H thymidine (TdR) uptake, induction of ornithine decarboxylase (ODC) and aryl hydrocarbon hydroxylase (AHH) was studied in primary embryonal mouse tongue cultures . (bvsalud.org)
- These results indicate that CYP1A2 is a member of the family of phenobarbital-inducible genes in mice and suggest that the aryl hydrocarbon receptor-dependent induction pathway is not involved in the induction of CYP1A2. (aspetjournals.org)
- The in vitro induction of the cytochrome P1-450-dependent monooxygenases, aryl hydrocarbon hydroxylase (AHH) or ethoxyresorufin O-deethylase (EROD) by 2,3,7,8-TCDD and related toxic halogenated aryl hydrocarbons in rat hepatoma H-4-II E cells has been developed as a short term quantitative bioassay for these toxic chemicals. (epa.gov)
- The resistance is accompanied by reduced sensitivity to induction of cytochrome P450 1A (CYP1A), a widely used biomarker of aromatic hydrocarbon exposure and effect, but whether the reduced sensitivity is specific to CYP1A or reflects a genome-wide reduction in responsiveness to all AHR-mediated changes in gene expression is unknown. (biomedcentral.com)
Carcinogens2
- It is unclear, however, whether cancer risk is related to the amount of red meat consumption per se or to certain meat-cooking practices, which produce mutagens and carcinogens such as heterocyclic amines (HCA), polycyclic aromatic hydrocarbons (PAH), and possibly other agents ( 2 - 6 ). (aacrjournals.org)
- The strongest carcinogens present in tobacco smoke are polycyclic aromatic hydrocarbons (PAH), N -nitrosamines, aromatic amines, aldehydes, benzene and butadiene. (mdpi.com)
Induced by aromatic hydrocarbons2
- in any mouse homozygous or heterozygous for the allele Ah , the monooxygenase activity is generally induced by aromatic hydrocarbons in many tissues regularly containing the inducible enzyme system. (aspetjournals.org)
- It is inhibited by hesperetin (a flavonoid found in lime, sweet orange), fluoroquinolones and macrolides and induced by aromatic hydrocarbons. (wikipedia.org)
CYP1B11
- These results suggest that CYP1B1 with polymorphic inducibility by dioxins is involved in aromatic hydrocarbon hydroxylase activities in human lymphocytes. (aacrjournals.org)
Mouse hepatoma line1
- Hankinson O. Single-step selection of clones of a mouse hepatoma line deficient in aryl hydrocarbon hydroxylase. (atcc.org)
Lymphocytes1
- We measured aryl hydrocarbon hydroxylase (AHH) in cultured human lymphocytes. (jax.org)
Inhibitor1
- A potent epoxide hydrase and aryl hydrocarbon hydroxylase inhibitor. (curehunter.com)
Activity7
- The half life of enzyme activity induced in vivo was related to the excretion of hydrocarbon metabolites. (unt.edu)
- Estradiol-2/4-hydroxylase (E-2/4-H) activity was determined in the mouse uterus during early pregnancy as well as in ovarian steroid hormone-treated ovariectomized uterus. (biomedsearch.com)
- Aryl hydrocarbon receptor-dependent inhibition of AP-1 activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin in activated B cells. (harvard.edu)
- Cholesterol 25-Hydroxylase Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication through Enzyme Activity-Dependent and -Independent Mechanisms. (bireme.br)
- Interestingly, a CH25H mutant (CH25H-M) lacking hydroxylase activity still inhibited PRRSV infection. (bireme.br)
- It is unclear as yet whether these findings have implications for the relationship between CYP2D6 genotype and in vivo debrisoquine 4-hydroxylase activity. (aspetjournals.org)
- Phenobarbital induced the expression of CYP1A2 at the levels of mRNA, protein, and enzyme activity (methoxyresorufin O -demethylation and metabolic activation of 2-amino-3-methylimidazo[4,5- f ]quinoline) in both aryl hydrocarbon-responsive [C57BL/6NCrj (C57BL/6), C3H/HeJSlc] and -nonresponsive (DBA/2NCrj, AKR/JSea, NZB/NSlc) mouse strains. (aspetjournals.org)
Tissues1
- 10. The Effects of Petroleum Hydrocarbon Exposure on the Structure of Fish Tissues. (elsevier.com)
Ligand activated1
- The aryl-hydrocarbon receptor (AHR), a ligand activated PAS superfamily transcription factor, mediates most, if not all, of the toxicity induced upon exposure to various dioxins, dibenzofurans, and planar polyhalogenated biphenyls. (hindawi.com)
Pathway2
- Among its related pathways are Estrogen Receptor Pathway and Aryl Hydrocarbon Receptor . (genecards.org)
- Populations of Atlantic killifish ( Fundulus heteroclitus ) have evolved resistance to the embryotoxic effects of polychlorinated biphenyls (PCBs) and other halogenated and nonhalogenated aromatic hydrocarbons that act through an aryl hydrocarbon receptor (AHR)-dependent signaling pathway. (biomedcentral.com)
Exposure2
- Blood carboxyhemoglobin, total particulate matter (TPM) intake, and pulmonary aryl hydrocarbon hydroxylase values indicated effective exposure of animals to cigarette smoke. (epa.gov)
- Journal Article] Fetal exposure to 2,3,7,8 'tetrachlorodibenzo-p-dioxin transactivates aryl hydrocarbon receptor-responsive element Ill in the tyrosine hydroxylase immunoreactive neurons of the mouse midbrain. (nii.ac.jp)
Petroleum hydrocarbons8
- Fate and Effects of Petroleum Hydrocarbons in Marine Organisms and Ecosystems is a collection of papers presented at the international symposium by the same title, held at the Olympic Hotel in Seattle, Washington on November 10-12, 1976. (elsevier.com)
- Part I deals with the inputs and physical transport processes influencing the distribution and composition of petroleum hydrocarbons in marine systems. (elsevier.com)
- 4. Biodegradation of Aromatic Petroleum Hydrocarbons. (elsevier.com)
- 5. Biotransformation of Petroleum Hydrocarbons in Marine Organisms Indigenous to the Arctic and Subarctic. (elsevier.com)
- 6. Accumulation and Turnover of Petroleum Hydrocarbons in Marine Organisms. (elsevier.com)
- 9. Responses to Sublethal Levels of Petroleum Hydrocarbons: Are they Sensitive Indicators and do they Correlate with Tissue Contamination? (elsevier.com)
- 11. The Effects of Petroleum Hydrocarbons on Marine Populations and Communities. (elsevier.com)
- 17. The Effect of Petroleum Hydrocarbons on the Survival and Life History of Polychaetous Annelids. (elsevier.com)
Biodegradation1
- 28. Arctic Hydrocarbon Biodegradation. (elsevier.com)
Protein3
- AHR-mediated signaling starts in the cytoplasm, where the receptor can be found in a complex with the heat shock protein of 90 kDa (Hsp90) and the immunophilin-like protein, aryl-hydrocarbon receptor-interacting protein (AIP). (hindawi.com)
- Upon ligand binding the AHR translocates to the nucleus and binds its heterodimeric partner, the aryl-hydrocarbon receptor nuclear translocator (ARNT) protein. (hindawi.com)
- Heterologous expression of CYP2K1 and identification of the expressed protein (Bv-2K1) as lauric acid (omega-1)-hydroxylase and aflatoxin B1 exo-epoxidase. (zfin.org)
Cells4
- Inhibitory effects of snuff extract on ornithine decarboxylase and aryl hydrocarbon hydroxylase activities in relation to cell proliferation of mouse tongue epithelial cells. (bvsalud.org)
- Polychlorinated biphenyl-induced apoptosis of murine spleen cells is aryl hydrocarbon receptor independent but caspases dependent. (harvard.edu)
- Many of these exposures include ligands of the aryl hydrocarbon receptor (AHR), a transcription factor expressed by immune and nonimmune cells. (physiology.org)
- Aryl hydrocarbon hydroxylase (AHH) activities are lower in G6PD-deficient cells, when tested in the absence of exogenous NADPH 2 . (springer.com)
Aromatic compounds1
- In the AhAh or Ahah mouse but not in the ahah mouse, the hydroxylase is induced by a variety of aromatic compounds: polycyclic hydrocarbons such as 3-methylcholanthrene or benz[ a ]anthracene, flavones, and 2-phenylbenzothiazoles. (aspetjournals.org)
Genetic1
- diphenylhydantoin hydroxylation may be involved with a different P-450 species regulated by an "aromatic hydrocarbon responsiveness" genetic locus other than the Ah locus. (aspetjournals.org)
Methylcholanthrene1
- Aryl hydrocarbon hydroxylase (AHH) was induced 15-fold in Ambystoma tigrinum by intraperitoneal injection of 3-methylcholanthrene in corn oil, or 10-fold by addition of aromatic polycyclic hydrocarbons to the aqueous environment of the neotene animal. (unt.edu)
Toxicity1
- The aryl hydrocarbon receptor (AHR) plays a central role not only in the toxicity of dioxins and other xenobiotics ( 1 - 3 ) but also in normal physiology ( 4 - 6 ). (pubmedcentralcanada.ca)
Cholesterol1
- Cholesterol 25-hydroxylase (CH25H) has recently been identified as a host restriction factor that exerts antiviral effects by catalyzing the production of 25-hydroxycholesterol (25HC). (bireme.br)
TCDD1
- The Ah receptor (AhR), once activated by ligands such as TCDD or planar aromatic hydrocarbons, translocates from the cytosol into the nucleus, heterodimerizes with Arnt (AhR nuclear translocator), and binds to a class of promoter DNA sequences called xenobiotic-responsive elements (XRE) (references 17 and 48 and references therein). (asm.org)
Ligands1
- Some of the developmental insults that have been associated with altered responses to infection later in life contain ligands for the aryl hydrocarbon receptor (AHR). (physiology.org)
Inhibition1
- Inhibition of the aryl hydrocarbon receptor prevents Western diet-induced obesity. (harvard.edu)