Biologically functional sequences of DNA chemically synthesized in vitro.
The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
The GENETIC RECOMBINATION of the parts of two or more GENES resulting in a gene with different or additional regulatory regions, or a new chimeric gene product. ONCOGENE FUSION includes an ONCOGENE as at least one of the fusion partners and such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS. ARTIFICIAL GENE FUSION is carried out in vitro by RECOMBINANT DNA technology.
The adherence and merging of cell membranes, intracellular membranes, or artificial membranes to each other or to viruses, parasites, or interstitial particles through a variety of chemical and physical processes.
The GENETIC RECOMBINATION of the parts of two or more GENES, including an ONCOGENE as at least one of the fusion partners. Such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS.
Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The GENETIC TRANSLATION products of the fusion between an ONCOGENE and another gene. The latter may be of viral or cellular origin.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Operative immobilization or ankylosis of two or more vertebrae by fusion of the vertebral bodies with a short bone graft or often with diskectomy or laminectomy. (From Blauvelt & Nelson, A Manual of Orthopaedic Terminology, 5th ed, p236; Dorland, 28th ed)
Proteins, usually glycoproteins, found in the viral envelopes of a variety of viruses. They promote cell membrane fusion and thereby may function in the uptake of the virus by cells.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
The functional hereditary units of BACTERIA.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.
Proteins found in any species of bacterium.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.
The locations in specific DNA sequences where CHROMOSOME BREAKS have occurred.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms.
Any method used for determining the location of and relative distances between genes on a chromosome.
DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.
A species of imperfect fungi from which the antibiotic fumigatin is obtained. Its spores may cause respiratory infection in birds and mammals.
Infections with fungi of the genus ASPERGILLUS.
A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.
Lung infections with the invasive forms of ASPERGILLUS, usually after surgery, transplantation, prolonged NEUTROPENIA or treatment with high-doses of CORTICOSTEROIDS. Invasive pulmonary aspergillosis can progress to CHRONIC NECROTIZING PULMONARY ASPERGILLOSIS or hematogenous spread to other organs.
Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.
Pulmonary diseases caused by fungal infections, usually through hematogenous spread.
Infections of the respiratory tract with fungi of the genus ASPERGILLUS. Infections may result in allergic reaction (ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS), colonization in pulmonary cavities as fungus balls (MYCETOMA), or lead to invasion of the lung parenchyma (INVASIVE PULMONARY ASPERGILLOSIS).
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A publication issued at stated, more or less regular, intervals.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
An acute myeloid leukemia in which 80% or more of the leukemic cells are of monocytic lineage including monoblasts, promonocytes, and MONOCYTES.
Cell surface receptors that bind GLYCINE with high affinity and trigger intracellular changes which influence the behavior of cells. Glycine receptors in the CENTRAL NERVOUS SYSTEM have an intrinsic chloride channel and are usually inhibitory.
A glycoprotein migrating as alpha 1-globulin, molecular weight 70,000 to 120,000. The protein, which is present in increased amounts in the plasma during pregnancy, binds mainly progesterone, with other steroids including testosterone competing weakly.
Myeloid-lymphoid leukemia protein is a transcription factor that maintains high levels of HOMEOTIC GENE expression during development. The GENE for myeloid-lymphoid leukemia protein is commonly disrupted in LEUKEMIA and combines with over 40 partner genes to form FUSION ONCOGENE PROTEINS.
A non-essential amino acid. It is found primarily in gelatin and silk fibroin and used therapeutically as a nutrient. It is also a fast inhibitory neurotransmitter.
The ordered rearrangement of gene regions by DNA recombination such as that which occurs normally during development.
The study of the structure, preparation, properties, and reactions of carbon compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
An octapeptide that is a potent but labile vasoconstrictor. It is produced from angiotensin I after the removal of two amino acids at the C-terminal by ANGIOTENSIN CONVERTING ENZYME. The amino acid in position 5 varies in different species. To block VASOCONSTRICTION and HYPERTENSION effect of angiotensin II, patients are often treated with ACE INHIBITORS or with ANGIOTENSIN II TYPE 1 RECEPTOR BLOCKERS.
The conformation, properties, reaction processes, and the properties of the reactions of carbon compounds.
Chemical and physical transformation of the biogenic elements from their nucleosynthesis in stars to their incorporation and subsequent modification in planetary bodies and terrestrial biochemistry. It includes the mechanism of incorporation of biogenic elements into complex molecules and molecular systems, leading up to the origin of life.
The reactions, changes in structure and composition, the properties of the reactions of carbon compounds, and the associated energy changes.
An angiotensin receptor subtype that is expressed at high levels in a variety of adult tissues including the CARDIOVASCULAR SYSTEM, the KIDNEY, the ENDOCRINE SYSTEM and the NERVOUS SYSTEM. Activation of the type 1 angiotensin receptor causes VASOCONSTRICTION and sodium retention.

Early induction of angiogenetic signals in gliomas of GFAP-v-src transgenic mice. (1/884)

Angiogenesis is a prerequisite for solid tumor growth. Glioblastoma multiforme, the most common malignant brain tumor, is characterized by extensive vascular proliferation. We previously showed that transgenic mice expressing a GFAP-v-src fusion gene in astrocytes develop low-grade astrocytomas that progressively evolve into hypervascularized glioblastomas. Here, we examined whether tumor progression triggers angiogenetic signals. We found abundant transcription of vascular endothelial growth factor (VEGF) in neoplastic astrocytes at surprisingly early stages of tumorigenesis. VEGF and v-src expression patterns were not identical, suggesting that VEGF activation was not only dependent on v-src. Late-stage gliomas showed perinecrotic VEGF up-regulation similarly to human glioblastoma. Expression patterns of the endothelial angiogenic receptors flt-1, flk-1, tie-1, and tie-2 were similar to those described in human gliomas, but flt-1 was expressed also in neoplastic astrocytes, suggesting an autocrine role in tumor growth. In crossbreeding experiments, hemizygous ablation of the tumor suppressor genes Rb and p53 had no significant effect on the expression of VEGF, flt-1, flk-1, tie-1, and tie-2. Therefore, expression of angiogenic signals is an early event during progression of GFAP-v-src tumors and precedes hypervascularization. Given the close similarities in the progression pattern between GFAP-v-src and human gliomas, the present results suggest that these mice may provide a useful tool for antiangiogenic therapy research.  (+info)

Conserved mechanism of PLAG1 activation in salivary gland tumors with and without chromosome 8q12 abnormalities: identification of SII as a new fusion partner gene. (2/884)

We have previously shown (K. Kas et al, Nat. Genet., 15: 170-174, 1997) that the developmentally regulated zinc finger gene pleomorphic adenoma gene 1 (PLAG1) is the target gene in 8q12 in pleomorphic adenomas of the salivary glands with t(3;8)(p21;q12) translocations. The t(3;8) results in promoter swapping between PLAG1 and the constitutively expressed gene for beta-catenin (CTNNB1), leading to activation of PLAG1 expression and reduced expression of CTNNB1. Here we have studied the expression of PLAG1 by Northern blot analysis in 47 primary benign and malignant human tumors with or without cytogenetic abnormalities of 8q12. Overexpression of PLAG1 was found in 23 tumors (49%). Thirteen of 17 pleomorphic adenomas with a normal karyotype and 5 of 10 with 12q13-15 abnormalities overexpressed PLAG1, which demonstrates that PLAG1 activation is a frequent event in adenomas irrespective of karyotype. In contrast, PLAG1 was overexpressed in only 2 of 11 malignant salivary gland tumors analyzed, which suggests that, at least in salivary gland tumors, PLAG1 activation preferentially occurs in benign tumors. PLAG1 over-expression was also found in three of nine mesenchymal tumors, i.e., in two uterine leiomyomas and one leiomyosarcoma. RNase protection, rapid amplification of 5'-cDNA ends (5'-RACE), and reverse transcription-PCR analyses of five adenomas with a normal karyotype revealed fusion transcripts in three tumors. Nucleotide sequence analysis of these showed that they contained fusions between PLAG1 and CTNNB1 (one case) or PLAG1 and a novel fusion partner gene, i.e., the gene encoding the transcription elongation factor SII (two cases). The fusions occurred in the 5' noncoding region of PLAG1, leading to exchange of regulatory control elements and, as a consequence, activation of PLAG1 gene expression. Because all of the cases had grossly normal karyotypes, the rearrangements must result from cryptic rearrangements. The results suggest that in addition to chromosomal translocations and cryptic rearrangements, PLAG1 may also be activated by mutations or indirect mechanisms. Our findings establish a conserved mechanism of PLAG1 activation in salivary gland tumors with and without 8q12 aberrations, which indicates that such activation is a frequent event in these tumors.  (+info)

A novel Vpr peptide interactor fused to integrase (IN) restores integration activity to IN-defective HIV-1 virions. (3/884)

A novel approach to complement human immunodeficiency virus type I (HIV-1) integrase (IN)-defective virions has been identified. The approach involves fusion of a 23-amino-acid stretch to the N-terminus of wild-type IN and coexpression of this chimera with the IN-defective proviral template in virus producing cells. The 23-amino-acid peptide represents a Vpr "interactor," referred to as the the WxxF or WF domain, which apparently leads to docking of the domain along with the fusion partner onto HIV-1 Vpr, thus permitting virion incorporation of the chimeric protein when expressed, in trans, with other viral products. Transfection of the WF-IN expression plasmid along with HIV-1 viral clones that produce Vpr, but bear an IN mutation, results in the release of a proportion of viral particles that are competent for integration. The extent of complementation was assessed using the MAGI cell assay, where integration of viral DNA results in the eventual appearance of easily visible multinucleated blue syncytia. The efficiency of dWF-IN (double copy of WF domain) complementation is not improved markedly by incorporation of a HIV-1 protease cleavage site (PR) between the dWF domain and IN (dWF-PR-IN), unlike that observed with Vpr fusions to IN. Furthermore, the ability of Vpr-PR-IN and dWF-PR-IN to complement IN-defective proviral clones, both of which bear an intervening protease cleavage site, appear comparable. Western blotting analyses using virions isolated through sucrose cushions demonstrate clearly the incorporation of the dWF-IN fusion protein into Vpr containing HIV-1 particles but not in Vpr-deficient virions. Additional Western blotting analyses indicate that all Vpr-IN and dWF-IN chimeras, with or without a PR site, are packaged into virions. The efficiency of virion incorporation of Vpr-IN and dWF-IN chimeras appears approximately comparable by Western blotting analysis. The ability of dWF-IN to complement IN-defective proviruses with efficiency similar to that of Vpr-PR-IN and dWF-PR-IN indicates that dWF-IN retains the full complement of functions necessary for integration of proviral DNA and is likely due to the benign nature of this small domain at the amino-terminus of IN.  (+info)

Insertion analysis of putative functional elements in the promoter region of the Aspergillus oryzae Taka-amylase A gene (amyB) using a heterologous Aspergillus nidulans amdS-lacZ fusion gene system. (4/884)

Expression of the Taka-amylase A gene (amyB) of Aspergillus oryzae is induced by starch or maltose. The A. oryzae amyB gene promoter contains three highly conserved sequences, designated Regions I, II, and III, compared with promoter regions of the A. oryzae glaA encoding glucoamylase and the agdA encoding alpha-glucosidase. To identify the function of these sequences within the amyB promoter, various fragments containing conserved sequences in the amyB promoter were introduced into the upstream region of the heterologous A. nidulans amdS gene (encoding acetamidase) fused to the Escherichia coli lacZ gene as a reporter. Introduction of the sequence between -290 to -233 (the number indicates the distance in base pairs from the translation initiation point (+1)) containing Region III significantly increased the expression of the lacZ reporter gene in the presence of maltose. The sequence between -377 to -290 containing Region I also increased the lacZ activity, but its maltose inducibility was less than that of Region III. The sequence between -233 to -181 containing Region II had no effect on the expression. These results indicated that Region III is most likely involved in the maltose induction of the amyB gene expression.  (+info)

Catabolic repression of secB expression is positively controlled by cyclic AMP (cAMP) receptor protein-cAMP complexes at the transcriptional level. (5/884)

SecB, a protein export-specific chaperone, enhances the export of a subset of proteins across cytoplasmic membranes of Escherichia coli. Previous studies showed that the synthesis of SecB is repressed by the presence of glucose in the medium. The derepression of SecB requires the products of both the cya and crp genes, indicating that secB expression is under the control of catabolic repression. In this study, two secB-specific promoters were identified. In addition, 5' transcription initiation sites from these two promoters were determined by means of secB-lacZ fusions and primer extension. The distal P1 promoter appeared to be independent of carbon sources, whereas the proximal P2 promoter was shown to be subject to control by the cyclic AMP (cAMP) receptor protein (CRP)-cAMP complexes. Gel-mobility shift studies showed that this regulation results from direct interaction between the secB P2 promoter region and the CRP-cAMP complex. Moreover, the CRP binding site on the secB gene was determined by DNase I footprinting and further substantiated by mutational analysis. The identified secB CRP binding region is centered at the -61.5 region of the secB gene and differed from the putative binding sites predicted by computer analysis.  (+info)

Pax6 and Pdx1 form a functional complex on the rat somatostatin gene upstream enhancer. (6/884)

The somatostatin upstream enhancer (SMS-UE) is a highly complex enhancer element. The distal A-element contains overlapping Pdx1 and Pbx binding sites. However, a point mutation in the A-element that abolishes both Pdxl and Pbx binding does not impair promoter activity. In contrast, a point mutation that selectively eliminates Pdx1 binding to a proximal B-element reduces the promoter activity. The B-element completely overlaps with a Pax6 binding site, the C-element. A point mutation in the C-element demonstrates that Pax6 binding is essential for promoter activity. Interestingly, a block mutation in the A-element reduces both Pax6 binding and promoter activity. In heterologous cells, Pdx1 potentiated Pax6 mediated activation of a somatostatin reporter. We conclude that the beta/delta-cell-specific activity of the SMS-UE is achieved through simultaneous binding of Pdx1 and Pax6 to the B- and C-elements, respectively. Furthermore, the A-element appears to stabilise Pax6 binding.  (+info)

Target joining of duplicated insertion sequence IS21 is assisted by IstB protein in vitro. (7/884)

Tandemly repeated insertion sequence IS21, located on a suicide plasmid, promoted replicon fusion with bacteriophage lambda in vitro in the presence of ATP. This reaction was catalyzed in a cell extract containing the 45-kDa IstA protein (cointegrase) and the 30-kDa IstB helper protein of IS21 after both proteins had been overproduced in Escherichia coli. Without IstB, replicon fusion was inefficient and did not produce the 4-bp target duplications typical of IS21.  (+info)

Host genes that affect the target-site distribution of the yeast retrotransposon Ty1. (8/884)

We report here a simple genetic system for investigating factors affecting Ty1 target-site preference within an RNAP II transcribed gene. The target in this system is a functional fusion of the regulatable MET3 promoter with the URA3 gene. We found that the simultaneous inactivation of Hir3 (a histone transcription regulator) and Cac3 (a subunit of the chromatin assembly factor I), which was previously shown by us to increase the Ty1 transposition rate, eliminated the normally observed bias for Ty1 elements to insert into the 5' vs. 3' regions of the MET3-URA3 and CAN1 genes. The double cac3 hir3 mutation also caused the production of a short transcript from the MET3-URA3 fusion under both repressed and derepressed conditions. In a hir3Delta single-mutant strain, the Ty1 target-site distribution into MET3-URA3 was altered only when transposition occurred while the MET3-URA3 fusion was actively transcribed. In contrast, transcription of the MET3-URA3 fusion did not alter the Ty1 target-site distribution in wild-type or other mutant strains. Deletion of RAD6 was shown to alter the Ty1 target-site preference in the MET3-URA3 fusion and the LYS2 gene. These data, together with previous studies of Ty1 integration positions at CAN1 and SUP4, indicate that the rad6 effect on Ty1 target-site selection is not gene specific.  (+info)

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mouse CORS26 protein: a secretory protein possibly involved in skeletal development; a C1q family member and growth factor; RefSeq NM_030888
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The GENETIC RECOMBINATION of the parts of two or more GENES resulting in a gene with different or additional regulatory regions, or a new chimeric gene product. ONCOGENE FUSION includes an ONCOGENE as at least one of the fusion partners and such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS. ARTIFICIAL GENE FUSION is carried out in vitro by RECOMBINANT DNA technology ...
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motivation= We aim at providing a clean, simple interface that will be self-intuitive and will allow researchers to submit high-quality scientific findings directly to a well established central repository (WormBase). Data will be publically shared and could be mined alongside data curated from the literature. This will ensure that every piece of federally funded research will be publicly available -including negative findings. We will pilot the submission focusing initially only on reporter gene fusions -the most common method to detect gene expression in c.elegans (WormBook chapter in preparation), and plan to expand the model to other gene expression localization experiments (e.g. ISH, smFISH, immunohistochemistry). If the model will prove to be successful, we will scale it up to include submission of other scientific findings (laser ablation experiments, rnai experiments..). guidelines for gene product localization experiments assessed with reporter gene fusions. The guidelines will be ...
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The effects of Prozyme knockout on blood form T. brucei.(A) Cell growth curves. Log cell number versus days in culture. Triangle, Flag-tagged prozyme Expressed
The information in the Mitelman Database of Chromosome Aberrations and Gene Fusions in Cancer relates cytogenetic changes and their genomic consequences, in particular gene fusions, to tumor characteristics, based either on individual cases or associations.
Participates in a DNA-damage check-point. DisA forms globular foci that rapidly scan along the chromosomes searching for lesions.
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With some 35,000 cattle, the Werthein familys beef business is one of the largest breeding set-ups in Argentina. Nick Fone paid them a visit
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... artificial gene fusion MeSH E05.393.220.870 - two-hybrid system techniques MeSH E05.393.281.526 - genetic complementation test ... artificial, heterologous MeSH E05.820.800.937.525 - insemination, artificial, homologous MeSH E05.820.800.968 - oocyte donation ... cell fusion MeSH E05.200.500.363 - cell separation MeSH E05.200.500.363.285 - cytapheresis MeSH E05.200.500.363.285.570 - ... gene therapy MeSH E05.393.420.451 - genetic enhancement MeSH E05.393.420.601 - protein engineering MeSH E05.393.420.601.035 - ...
These fusion linkers code for a 15 amino acid glycine and serine polypeptide, which is an ideal linker peptide for fusion ... Artificial gene synthesis, or gene synthesis, refers to a group of methods that are used in synthetic biology to construct and ... Increasingly, genes are ordered in sets including functionally related genes or multiple sequence variants on a single gene. ... Because artificial gene synthesis does not require template DNA, it is theoretically possible to make a completely synthetic ...
ChIA-PET is similar to finding fusion genes through RNA-PET in that the paired tags map to different regions in the genome. ... However, ChIA-PET involves artificial ligations between different DNA fragments located at different genomic regions, rather ... RNA-PET could also be used to detect fusion genes and trans-splicing, but further experiment is needed to distinguish between ... RNA-PET: This application is used for studying the transcriptome: transcripts, gene structures, and gene expressions. The PET ...
The yeast two-hybrid and bacterial two-hybrid screen investigate the interaction between artificial fusion proteins. They do ... The cells are designed in a way that an interaction activates the transcription of a reporter gene or a reporter enzyme. Most ... Applied in vivo, FRET has been used to detect the location and interactions of genes and cellular structures including ...
Thus, CRISP-Disp enables control of gene expression with deployment of both artificial scaffolds as well as natural lncRNA ... CRISPR-Display can also recruit fusion proteins engineered to bind specific RNA sequences. Recruiting these proteins can allow ... Artificial ncRNA functionalization[edit]. CRISPR-Display also allows for targeted use of the wide array of artificial RNAs with ... To determine if artificial lncRNA scaffolds can be used with CRISPR-Display, dCas9 complexes were assembled with artificial RNA ...
"Gene prioritization by compressive data fusion and chaining". PLoS Computational Biology 11(10):e1004552, 2015. Martin Stražar ... Blaž Zupan teaches artificial intelligence, machine learning, data mining and bioinformatics at the University of Ljubljana and ... His research focuses on constructive induction, epistasis approaches for reconstructing gene networks, large-scale data fusion ... "Data fusion by matrix factorization". IEEE Transactions on Pattern Analysis and Machine Intelligence 37(1):41-53, 2015. Marinka ...
These fusion proteins serve as readily targetable "DNA scissors" for gene editing applications that enable to perform targeted ... TALENs are artificial restriction enzymes designed by fusing the DNA cutting domain of a nuclease to TALE domains, which can be ... Targeted gene mutation Gene therapy Creating chromosome rearrangement Study gene function with stem cells Transgenic animals ... If a vital gene is knocked out it can prove lethal to the organism. In order to study the function of these genes site specific ...
... some gene fusions may cause regulatory changes that alter when and where these genes act. For partial gene fusions, the ... can be manufactured through genetic engineering by fusion to the original proteins of peptide domains that induce artificial ... Examples include: Gag-onc fusion protein Bcr-abl fusion protein Tpr-met fusion protein Antibodies are fusion proteins produced ... A recombinant fusion protein is a protein created through genetic engineering of a fusion gene. This typically involves ...
Jefferson RA, Kavanagh TA, Bevan MW (December 1987). "GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion ... "Artificial Genes". TIME. 15 November 1982. Retrieved 17 July 2010. Horn ME, Woodard SL, Howard JA (May 2004). "Plant molecular ... Some genes are only expressed in certain tissues, so reporter genes, like GUS, can be attached to the gene of interest allowing ... Gene therapy uses genetically modified viruses to deliver genes which can cure disease in humans. Although gene therapy is ...
The proteins themselves are fusion transcription factors, which can target particular genes. She hopes to increase the use of ... her experience with chromatin dynamics and synthetic biology to create artificial transcription factors which activated genes ... "A Sensitive Switch for Visualizing Natural Gene Silencing in Single Cells". ACS Synthetic Biology. 1 (3): 99-106. doi:10.1021/ ... grant to study the use of modular peptide motifs to build synthetic chromatin proteins that activate dormant therapeutic genes ...
It was also by means of cell fusion that Harris and Goss devised the first systematic method for determining the order of genes ... Loyd-Luke, London (pub). ISBN 978-0-85324-054-9 Hybrid Cells Derived from Mouse and Man : Artificial Heterokaryons of Mammalian ... These genes are now known as tumour suppressor genes and work on them has become a world-wide industry. Harris's research was ... With John Watkins he developed the technique of cell fusion for the study of the physiology and genetics of higher cells. They ...
Methods of artificial transfection include (a) chemical methods, including calcium phosphate precipitation, DEAE-dextran ... Targeted introduction of exogenous DNA is used to identify genes because the introduced DNA can act cause a mutation or altered ... Fusion of this lipid and the membrane lipid can occur, facilitating the entry of DNA. ... These include the study of developmental genetics, disease processes and gene regulation. Transgenic farm animals can produce ...
BiTE is a registered trademark of Micromet AG (fully owned subsidiary of Amgen Inc). BiTEs are fusion proteins consisting of ... Bi-specific T-cell engagers (BiTEs) are a class of artificial bispecific monoclonal antibodies that are investigated for the ... two single-chain variable fragments (scFvs) of different antibodies, or amino acid sequences from four different genes, on a ...
... have been developed using the molecular biology technique of gene fusion, a process that links the expression of the ... For example, one strategy often in use is the artificial production of proteins, based on the genetic code (DNA). These ... it creates an artificial phase difference of about a quarter wavelength. As the physical properties of this direct light have ...
Eugene Boland is the Chief Scientist at Techshot, a company located in Greenville, Indiana. They intend to send small canisters ... It would be located at the Mars Lagrange orbit L1 at about 320 R♂, creating a partial and distant artificial magnetosphere. The ... National Institute for Fusion Science (Japan). Retrieved June 7, 2016. "Policy, Pathways, Techniques, and Capabilities - from ... Some scientists hypothesize that creating a planet-wide artificial magnetosphere would be helpful in resolving this issue. ...
In standard synthetic biology, artificial gene networks are introduced into cells, inputs (e.g. chemicals, light) are applied ... cell fusion, cell locomotion, chemotaxis, haptotaxis, cell wedging). Broadly similar lists exist for tissues of plants, fungi ... idea by adding output modules that alter the shape or social behaviour of cells in response to the state of the artificial gene ... For example, instead of just making a fluorescent protein, a gene network may switch on an adhesion molecule so that cells ...
... fusion protein - gel electrophoresis - gel shift - gel shift assay - gene - gene amplification - gene conversion - gene ... bacterial artificial chromosome - bacteriophage - bacteriophage lambda - band shift assay - base - base pair - binding site - ... expression - gene mapping - gene pool - gene therapy - gene transfer - genetic code - genetic counseling - genetic map - ... candidate gene - Canonical sequence - cap - cap site - carboxyl terminus - carcinoma - carrier - CAT assay - CCAAT box - cDNA ...
In humans this is encoded by the SFTPC gene. It is a membrane protein. SFTPC is a 197-residue protein made up of two halves: a ... Recombinant SP-C is used in Venticute, an artificial lung surfactant. A process to mass-produce an analogue called rSP-C33Le by ... fusion with spidroin has been described. GRCh38: Ensembl release 89: ENSG00000168484 - Ensembl, May 2017 GRCm38: Ensembl ... "Entrez Gene: SFTPC surfactant, pulmonary-associated protein C". Willander H, Askarieh G, Landreh M, Westermark P, Nordling K, ...
When ttx-7 mutants also had a mutant egl-8 gene, the defects caused by the faulty ttx-7 gene were largely reversed. These ... Artificial Intelligence in the Age of Neural Networks and Brain Computing. Elsevier. pp. 1-30. doi:10.1016/b978-0-12-815480- ... and direct inhibition of the vesicle fusion process. Endocannabinoids, synthesized in and released from postsynaptic neuronal ... A gene (ttx-7) was identified in Caenorhabditis elegans that encodes myo-inositol monophosphatase (IMPase), an enzyme that ...
... as well as candidate gene-fusion events. Trinity first divides the sequence data into a number of de Bruijn graphs, each ... Velvet can produce contigs up to 50-kb N50 length using prokaryotic data and 3-kb N50 in mammalian bacterial artificial ... It is also possible that they represent different genes within the same gene family, or even genes that share only a conserved ... To perform this step, tools that model the expected gene space based of conserved genes, such as BUSCO, can be used. For ...
The late Eugene Mallove, cold fusion advocate. The late Jim Marrs, author mostly known for "Crossfire", a discussion of the ... Kevin Warwick, professor of cybernetics who discusses his research with implants, artificial intelligence and robotics - ...
"Gene Designer: A synthetic biology tool for constructing artificial DNA segments". BMC Bioinformatics. 7: 285. doi:10.1186/1471 ... Remove or add restriction sites or other sequence motifs Recode open reading frames Check translation frames and fusion ... Gene Designer enables molecular biologists to manage the full gene design process in one application, using a range of design ... Gene Designer is a computer software package for bioinformatics. It is used by molecular biologists from academia, government, ...
Combining these genes with an array of genes for other domains of the antibody generates a large cavalry of antibodies with a ... They are usually artificial peptides or proteins with a molar mass of about 3 to 20 kDa. Nucleic acids and small molecules are ... In some cases, additional fusions with other lines have created "triomas" and "quadromas". The manufacturing process should be ... one D and one J gene segment (or one V and one J segment in the light chain). As there are multiple copies of each type of gene ...
... s appear to have evolved through gene duplication and domain fusion events of half-barrel proteins, with a majority ... Höcker B, Lochner A, Seitz T, Claren J, Sterner R (February 2009). "High-resolution crystal structure of an artificial ( ... An ancestral half-barrel would have undergone a gene duplication and fusion event, resulting in a single protein containing two ... The predominant theory for TIM barrel evolution involves gene duplication and fusion, starting with a half- barrel that ...
"Artificial intelligence helps accelerate progress toward efficient fusion reactions". Phys.org. 15 December 2017. Retrieved 24 ... The CDH2 gene is found to be implicated in sudden death among young people and athletes. A study by the Harvard-Smithsonian ... "Artificial intelligence helps accelerate progress toward efficient fusion reactions". Princeton University. 15 December 2017. ... "Fusion heating gets a boost". MIT. 21 August 2017. Retrieved 29 August 2017. "MIT Achieves Breakthrough in Nuclear Fusion". ...
Gene McFalls of the USAF has said that sensor fusion will feed into inventory databases to precisely identify aircraft at a ... Sukhoi calls their expert system for sensor fusion the artificial intelligence of the Su-57. Flight tests of their integrated ... Sensor fusion and automatic target tracking are projected to give the fifth-generation jet fighter pilot a view of the ... By the end of 2013 the biggest concern with the F-35 program was software, especially the software required to do data fusion ...
The total genome size of O. volvulus is 1.5x108 BP and contains around 4,000 genes, with genes for collagen and cuticular ... Artificial water systems, such as hydroelectric power plants, built in Africa, provide ideal conditions all year for blackfly ... One of the three nonsex chromosomes is thought to have formed by a fusion event between two smaller chromosomes. (Simplified ... It also shows high haplotype diversity, which is a measure of how unique a group of linked genes is. This pattern of low ...
In addition to gene and drug delivery applications, liposomes can be used as carriers for the delivery of dyes to textiles, ... These liposomes work to deliver drug by diffusion rather than by direct cell fusion. A similar approach can be exploited in the ... Liposomes are used as models for artificial cells. Liposomes can also be designed to deliver drugs in other ways. Liposomes ... Cevc, G; Richardsen, H (1993). "Lipid vesicles and membrane fusion". Advanced Drug Delivery Reviews. 38 (3): 207-232. doi: ...
TALEN fusions show activity in a yeast-based assay, at endogenous yeast genes, in a plant reporter assay, at an endogenous ... have been used to create artificial transcription factors that can be used to target and activate or repress endogenous genes ... plant gene, at endogenous zebrafish genes, at an endogenous rat gene, and at endogenous human genes. The human HPRT1 gene has ... gene family. These genes are essential for the development of the disease. In rice two genes,Os-8N3 and Os-11N3, are induced by ...
RNA probes can be designed for any gene or any sequence within a gene for visualization of mRNA,[3][4][5] lncRNA[6][7][8] and ... Hybrid Fusion-FISH[edit]. Hybrid Fusion FISH (HF-FISH) uses primary additive excitation/emission combination of fluorophores to ... The artificial chromosomes (BAC) can be grown, extracted, and labeled, in any lab containing a library. Genomic libraries are ... The technology has potential applications in cancer diagnosis,[14] neuroscience, gene expression analysis,[15] and companion ...
Artificial photosynthesis. *Biofuels. *Carbon-neutral fuel. *Concentrated solar power. *Fusion power. *Home fuel cell ... Gene therapy. *Head transplant. *Isolated brain. *Life extension *Strategies for Engineered Negligible Senescence ...
Eugene Paul Wigner; Andrew Szanton (1992). Andrew Szanton, ed. The Recollections of Eugene P. Wigner As Told to Andrew Szanton ... He played a fundamental role in the first experimental demonstration of nuclear fusion and also the development of the atomic ... He is the author of several books on health, artificial intelligence (AI), transhumanism, the technological singularity, and ... Eugene Dynkin (1924-2014): Soviet and American mathematician. He has made contributions to the fields of probability and ...
Artificial. Medical. 0.60. 0.03-2.0. 3.00. 0.53. 2.30. Fallout. 0.007. 0 - 1+. -. -. 0.01. Peaked in 1963 with a spike in 1986 ... And he proposed a theory that they were produced in interstellar space as by-products of the fusion of hydrogen atoms into the ... Shielding Space Travelers by Eugene Parker.. *Anomalous cosmic ray hydrogen spectra from Voyager 1 and 2 ... Charged particles of such high energy could not possibly be produced by photons from Millikan's proposed interstellar fusion ...
... viable daughter cells to be produced would require an extra replication of the intact RNA gene homologous to any RNA gene that ... The periodic fusions permit mutual reactivation of otherwise lethally damaged protocells. If at least one damage-free copy of ... Tucker BJ, Breaker RR (Jun 2005). "Riboswitches as versatile gene control elements". Current Opinion in Structural Biology. 15 ... Lamb RA, Choppin PW (1983). "The gene structure and replication of influenza virus". Annual Review of Biochemistry. 52: 467-506 ...
Connecting two optical fibers is done by fusion splicing or mechanical splicing and requires special skills and interconnection ... Artificial intelligence *Applications of artificial intelligence. *Progress in artificial intelligence. *Machine translation ... Gene therapy. *Head transplant. *Isolated brain. *Life extension *Strategies for Engineered Negligible Senescence ...
The invention of the nuclear reactor in the Manhattan Project during World War 2 made possible the production of artificial ...
Homologs of the recA gene, including rad51, play a key role in homologous recombinational repair of DNA during meiosis. A rad51 ... 3 October 2014), "Appraisal of artificial screening techniques of tomato to accurately reflect field performance of the late ... hybrids of tomato and diploid potato can be created in the lab by somatic fusion, and are partially fertile,[15] providing ... The C.M. Rick Tomato Genetics Resource Center at UC Davis is a gene bank of wild relatives, monogenic mutants and miscellaneous ...
... each piece of RNA containing either one or two genes, which code for a gene product (protein).[63] For example, the influenza A ... or artificial) UV radiation. This could explain why influenza occurs mostly in winter and during the tropical rainy season, ... "An open receptor-binding cavity of hemagglutinin-esterase-fusion glycoprotein from newly-identified Influenza D Virus: Basis ... Lamb RA, Choppin PW (1983). "The gene structure and replication of influenza virus". Annu. Rev. Biochem. 52: 467-506. doi: ...
Willis Eugene Lamb. Harold Lewis. Philip Morrison. Arnold Nordsieck. Melba Phillips. Hartland Snyder. George Volkoff. ... With his first doctoral student, Melba Phillips, Oppenheimer worked on calculations of artificial radioactivity under ... nuclear fusion-based hydrogen bomb, then known as "the Super".[138] Oppenheimer had been aware of the possibility of a ... the Oppenheimer-Phillips process in nuclear fusion, and the first prediction of quantum tunneling. With his students he also ...
This band was once[when?] held to be less damaging to DNA, and hence is used in cosmetic artificial sun tanning (tanning booths ... Although the Sun produces gamma rays as a result of the nuclear-fusion process, internal absorption and thermalization convert ... June 15, 2005). "Sun Exposure, Vitamin D Receptor Gene Polymorphisms, and Risk of Advanced Prostate Cancer". Cancer Research. ... "Artificial Tanning Booths and Cancer - National Cancer Institute". 31 January 2013. Retrieved 25 January 2018.. ...
Many people put up bat houses to attract bats.[230] The 1991 University of Florida bat house is the largest occupied artificial ... Several species have a fission-fusion social structure, where large numbers of bats congregate in one roosting area, along with ... gene from bats (Chiroptera)". Genetics Research. 91 (2): 101-9. doi:10.1017/s0016672309000032. PMID 19393126.. ... the gene controlling Bmp signalling, Bmp2, is subjected to increased expression in bat forelimbs-resulting in the extension of ...
... resistance gene and can explicit approximately 30% of average efficiency having successful T-DNA inserts induced gene fusions ... "Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector ... For example, this gene disruption strategy used for assigning functions to genes defined only by sequence helped to demonstrate ... In genetic engineering, the tumor-promoting and opine-synthesis genes are removed from the T-DNA and replaced with a gene of ...
15th Annual Conference on Computational Learning Theory (COLT 2002), Sydney, Australia, Lecture Notes in Artificial ... and nuclear fusion would have converted all hydrogen in the early universe to helium. Water, as well as sufficiently long-lived ... which is an interpretation of quantum mechanics associated with the ideas of John von Neumann and Eugene Wigner.. *"An ensemble ...
Fusion splicing is done with a specialized instrument. The fiber ends are first stripped of their protective polymer coating ( ... Eugene G, eds. "Optical and surface properties of oxyfluoride glass". Proceedings of SPIE. Inorganic Optical Materials II. 4102 ... toys and artificial Christmas trees. Swarovski boutiques use optical fibers to illuminate their crystal showcases from many ... For applications that demand a permanent connection a fusion splice is common. In this technique, an electric arc is used to ...
In other words, although one gene (GRIA1-GRIA4) is encoded for each subunit (GluA1-GluA4), splicing after transcription from ... Its name is derived from its ability to be activated by the artificial glutamate analog AMPA. The receptor was first named the ... The GluA2 C terminus interacts with and binds to N-ethylmaleimide sensitive fusion protein,[53][54][55] which allows for the ... DNA allows some exons to be translated interchangeably, leading to several functionally different subunits from each gene. ...
The IBM Blue Gene/P computer has been used to simulate a number of artificial neurons equivalent to approximately one percent ... and nuclear fusion). They have been essential in the field of cryptanalysis.[6] ... the Blue Gene/Q reached 1,684 MFLOPS/W.[71][72] In June 2011 the top 2 spots on the Green 500 list were occupied by Blue Gene ... In the Blue Gene system, IBM deliberately used low power processors to deal with heat density.[65] The IBM Power 775, released ...
Most of the genes responsible for the regulation of inner ear formation and its morphogenesis are members of the homeobox gene ... Sometimes artificial ear bones are placed to substitute for damaged ones, or a disrupted ossicular chain is rebuilt in order to ... The pinna originates as a fusion of six hillocks. The first three hillocks are derived from the lower part of the first ... Chatterjee, Sumantra; Kraus, Petra; Luftkin, Thomas (2010). A symphony of inner ear developmental control genes. Retrieved 20 ...
... in which one gene segment recombines with other gene segments to form a single unique gene. This assembly process generates the ... Also, viral fusion proteins cause the formation of the placental syncytium[23] to limit exchange of migratory cells between the ... Both actively acquired and passively acquired immunity can be obtained by natural or artificial means. ... Genes involved in antigen processing and presentation, as well as the class I and class II genes, are closely linked within the ...
Eugene Wigner proposed to use the 240Pu-contaminated plutonium to drive the conversion of thorium into 233U in a special ... 229Th is mostly produced as a daughter of artificial 233U made by neutron irradiation of 232Th, and is extremely rare in nature ... Coulomb barriers that make interactions between charged particles difficult at high atomic numbers and the fact that fusion ...
Singularity Institute for Artificial Intelligence (2002), Why Artificial Intelligence?, archived from the original on October 4 ... Bethe understood nuclear fusion in 1935, but 75 years later fusion reactors are still only used in experimental settings. ... The next step would be to apply this technology to gene therapy.[71] Kurzweil's point is that SGT provides the best example of ... Singularity Institute for Artificial Intelligence 2004) *^ Kurzweil, Ray (2005). The Singularity is Near. New York: Penguin ...
... since they effectively see the human's movements in slow motion with their higher flicker fusion rate.[4][5] ... "Gene Flow Among Geographically Diverse Housefly Populations (Musca domestica L.): A Worldwide Survey of Mitochondrial ...
... transcriptomics have allowed researchers to comprehensively analyze gene transcription in beta-cells to look for genes linked ... These variations of artificial beta cells have shown greater levels of success in replicating the functionality of natural beta ... and the opening of voltage gated calcium channels causing insulin granule fusion and exocytosis.[7] ... The insulin gene is first transcribed into mRNA and translated into preproinsulin.[3] After translation, the preproinsulin ...
Avantaggiato, A; Girardi, A; Palmieri, A; Pascali, M; Carinci, F (August 2015). "Bio-Revitalization: Effects of NASHA on Genes ... its fusion with primary lysosomes, enzymatic digestion to monosaccharides, active transmembrane transport of these sugars to ... Hyaluronic acid has been used in various formulations to create artificial tears to treat dry eye.[44] ... TNF-stimulated gene 6) in fibroblasts and inflammatory cells. TSG-6, a HA-binding protein, also forms a stable complex with the ...
Judéa Péarl, "Fusion, propagation, and structuring in belief networks". Artificial Intelligence, 29(3):241-288, 1986. ... Jaringan Bayes geus dipaké keur pangaweruh model dina gene regulatory network, medicine, rékayasa, text analysis, image ...
"Gene therapy is first deafness 'cure' - health - 14 February 2005 - New Scientist".. ... Scaffolds composed of natural and artificial components are seeded with mesenchymal stem cells and placed in the defect. Within ... Because subsequent viral replication requires cellular gene expression processes, activated CD4+ cells are the primary targets ... gene-modified (HIV-1-resistant) hematopoietic stem and progenitor cells (GM-HSPC).[57] ...
If the corundum is lacking in chromium(III) ions, it is known as a sapphire.[note 3] A red-colored artificial ruby may also be ... Mechanisms include mutagenic gene damage and disruptions of enzyme functions.[129]. There is evidence that fish may not require ... Heat of fusion. 21.0 kJ/mol Heat of vaporization. 347 kJ/mol ... achieved by doping chromium(III) into artificial corundum ...
Spine fusions maybe performed as open surgery or as minimally invasive surgery. Anterior cervical diskectomy and fusion is a ... This is used in functional neurosurgery where electrodes are implanted or gene therapy is instituted with a high level of ... Procedures such as microdiscectomy, laminectomy, and artificial disc replacement rely on microsurgery.[14] ... Generally once a disc is removed it is replaced by an implant which will create a bony fusion between vertebral bodies above ...
This has been done on a strain of Nostoc punctiforme: one of the structural genes of the NiFe uptake hydrogenase was ... "Artificial Photosynthesis". Csiro.au. 20 September 2005. Retrieved 2011-04-19.. *^ "Designing a Better Catalyst for Artificial ... Towards Global Artificial Photosynthesis Lord Howe Island 2011 "Artificial Photosynthesis". Archived from the original on 28 ... Paul Alivisatos on Artificial Photosynthesis at Lawrence Berkeley National Laboratory. *Nanocapsules for artificial ...
Artificial Gene Fusion * Genes, Reporter * Interferons / antagonists & inhibitors* * Luciferases / biosynthesis * Luciferases ... The V proteins were more potent inhibitors of IFN-inducible reporter gene expression than the C proteins, and this effect was ...
Artificial Gene Fusion * B-Lymphocytes * Bone Marrow / pathology * Cell Line * Chromosome Mapping* ... Evidence for at least eight Fanconi anemia genes Am J Hum Genet. 1997 Oct;61(4):940-4. doi: 10.1086/514881. ... These results thus define three new groups, FA-F, FA-G, and FA-H, providing evidence for a minimum of eight distinct FA genes. ... Complementation groups in FA are likely to represent distinct disease genes, two of which (FAC and FAA) have been cloned. ...
The use of yeast artificial chromosomes (YACs) containing long stretches of human DNA allows dispersed DNA markers to be used ... We describe a procedure allowing rapid isolation of cDNAs corresponding to genes within a YAC clone. Random cDNA is hybridised ... but does not readily allow subcloning of the precise breakpoint within the YAC DNA nor the cDNAs containing the affected genes ... A method for identifying genes within yeast artificial chromosomes: application to isolation of MLL fusion cDNAs from acute ...
Artificial Gene Fusion. Aspergillosis / microbiology, pathology. Aspergillus fumigatus / drug effects*, genetics, growth & ... fumigatus strain by fusing the promoter of the glyceraldehyde-3-phosphate dehydrogenase gene from A. fumigatus with the ... luciferase gene from Photinus pyralis to control the expression of the bioluminescent reporter. A. fumigatus transformed with ...
Artificial Gene Fusion. The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE ... Fertilization is the consequence of the fusion of two haploid gametes, the oocyte and the sperm. In mammals, such as human and ...
Chinas artificial sun reaches fusion temperature: 100 million degrees In a breakthrough for nuclear fusion research, ... Twins Research Shows Genes Help Cast Your Vote A new study out of the UK determined that an inclination to vote for certain ... Below, geneticist Bryan Sykes offers a basic lesson in genetics by comparing your genes to a deck of playing cards: ... Spector goes as far as to say that additional research suggests our genes have much more influence on our personalities than ...
NameArtificial Gene Fusion. Tree numbers*E05.393.220.250. MT-h12769 , LY6E 3UTR Lenti-reporter-Luc Vector size: 1 ug , 400.77 ... Alternative to gene target lymphocyte antigen 6 complex, locus E, RIG-E and RIGE and SCA-2 and SCA2 and TSA-1, LY6E and IDBG- ... They are one of the most efficient methods of a gene delivery vector. The are packaged in HEK 293T cells to get the highest ... The Luc reporter is the expression of the firefly luciferase gene. Puro stands for puromicin selection. ...
NameArtificial Gene Fusion. Tree numbers*E05.393.220.250. MT-h07025 , ERMAP 3UTR Lenti-reporter-Luc Vector size: 1 ug , 534.38 ... They are one of the most efficient methods of a gene delivery vector. The are packaged in HEK 293T cells to get the highest ... Alternative to gene target erythroblast membrane-associated protein (Scianna blood group), ERMAP and IDBG-97277 and ... The Luc reporter is the expression of the firefly luciferase gene. Puro stands for puromicin selection. ...
Efficient recombination-based methods for bacterial artificial chromosome fusion and mutagenesis. Gene. 2006;371(1):136-143. [ ... A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature. 2003;425(6961):917-925 ... Construction of human artificial chromosome vectors by recombineering. Gene. 2005;351:29-38. [PubMed] ... Gene expression from bacterial artificial chromosome (BAC) clones has been demonstrated to facilitate physiologically relevant ...
Nuclear energy - fission or artificial fusion - bleeds the fabric of THEIR plenum and genes. THEY know there is no free lunch! ... JIM GARRISON; New Orleans D.A. who tried to get Eugene Brading out of L.A. (but used one of Bradings other aliases, Eugene ... The Hopi have a name for the gravity wind in that (Gene-tic Gravity) worm -it is called PESHMEHTEN - One Less than TEN (not ... The Fateful Century B.C. (ab-RA-ham : Enlil / RA breathed into the families genes. More on Enlil the murderous: The Shining ...
Artificial Gene Fusion*. *Bacterial Outer Membrane Proteins/immunology*. *Immunization, Secondary*. *Leptospira/immunology* ... The chosen genes were amplified by PCR methodology and cloned into pAE, an E. coli vector. The recombinant proteins were ... The chosen genes were amplified by PCR methodology and cloned into pAE, an E. coli vector. The recombinant proteins were ... Induction of boosted immune response in mice by leptospiral surface proteins expressed in fusion with DnaK. ...
In MLL-bacterial lacZ fusion knock-in mice, the artificial fusion gene causes leukemia, as do the naturally occurring fusion ... Our patient revealed a novel MLL-partner gene fusion along with a de facto truncated MLL gene. Further studies on this ... Alternative splicing of human gephyrin gene created two different forms of fusion cDNA. In one form, human gephyrin gene fused ... The human gephyrin gene is highly homologous to the rat gephyrin gene. The rat gephyrin gene encoding a Mr 93,000 protein was ...
News Link • Robots and Artificial Intelligence Watch tiny robots swim through an eyeball to deliver medicine. Robert Lee ... Scientists unravel role of fusion gene in prostate cancer 05-22-2012. • medicalxpress.com, byNew York- Presbyterian Hosp. These ... "This is likely a phenomenon that occurs in many types of cancers when oncogenic fusion genes are over-expressed," says the ... Rubin adds that if such an oncogenic protein has the power to throw the switch on thousands of genes, a novel treatment may be ...
The construction and cloning of synthetic genes coding for artificial proteins and expression studies to obtain fusion proteins ... Expression of synthetic genes coding for completely new, nutritionally rich, artificial proteins. Jacek Biernat, Hubert Köster ... The construction, cloning, and expression of synthetic genes coding for totally artificial proteins. Hubert Köster, Jacek ... Synthetic food protein genes and their expression, and use of the protein in diets for treatment of liver insufficiency and ...
Artificial intelligence could yield more accurate breast cancer diagnoses 08/05/2019 Pancreatic Cancer ... The hybrid gene is a fusion of two separate genes, and is one of the first described in gastric cancer, which is the most ... Finding the error led the scientists to the CD44-SLC1A2 fusion gene, which resulted when two nearby genes blended into one. The ... "This is an extremely exciting area, as it opens up a potential role for fusion genes in solid cancer diagnostics and treatment ...
... this thesis also focussed on the cell stress caused by an artificial and very strong RNA psudoknot used in a fusion gene. The ... a response that suggested the pseudoknot construct possibly conferred misfolding of the fusion gene or were unable to release ... It was demonstrated that typical genes associated with the heat stress response were upregulated - ... suggesting Rho transcriptional pre-termination to be a global phenomena in gene regulation.. The in uence of codon usage in the ...
ARTIFICIAL GENE FUSION is carried out in vitro by RECOMBINANT DNA technology. ... Gene Fusion. The GENETIC RECOMBINATION of the parts of two or more GENES resulting in a gene with different or additional ... ONCOGENE FUSION includes an ONCOGENE as at least one of the fusion partners and such gene fusions are often detected in ... Such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS. ...
Recycling atmospheric CO2 into liquid methanol using artificial marine floating islands. ... FRAG1, a gene that potently activates fibroblast growth factor receptor by C-terminal fusion through chromosomal rearrangement ... FRAG1, a gene that potently activates fibroblast growth factor receptor by C-terminal fusion through chromosomal rearrangement ... FRAG1, a gene that potently activates fibroblast growth factor receptor by C-terminal fusion through chromosomal rearrangement ...
Scroll down to his description of his organisations current research on artificial chromosomes. ... to make synthetic genes and that those genes would be unique genes- she said they would be like plastic (because plastic ... keeping existing genes functional (if thats the goal), plus some genes end up lethal to a cell. We can do a lot more in ... you can just use it by using it as a synthetic man-made gene (Since you couldnt use it as a non-synthetic gene Since it ...
Using 2-node hypergraph clustering coefficients to analyze disease-gene networks ... Fusion, Propagation, and Structuring in Belief Networks by Judea Pearl - ARTIFICIAL INTELLIGENCE , 1986 ... by T. S. Eugene Ng, Hui Zhang - In INFOCOM , 2001 "... In this paper, we propose to use coordinates-based mechanisms in a peer- ...
Numerous studies have revealed the potential value of gene expression signatures in examining the risk of disease recurrence. ... The invention of microarray technology and its ability to simultaneously interrogate thousands genes has changed the paradigm ... concentrating precisely on clinical and gene expression profiles. The literature is reviewed in an explicit machine learning ... "Fusion, propagation and structuring in belief networks". Artificial Intelligence, 29: 241-288, 1986.. ...
To examine the oncogenic activity of ASPSCR1 fusion genes, artificial chimeric genes were generated consisting of the 5′ ... and in-frame fusions were confirmed by sequencing. The FLAG-tagged full-length coding sequences of each fusion genes were ... TFE3 is fused to ASPSCR1 and several other genes in renal cell carcinoma, whereas TFEB is a component of fusion genes in a ... Artificial chimeric ASPSCR1 fusions between ASPSCR1 and TFEB, TFEC, or MITF were also generated by two-step PCR using primers ...
Apoptosis Regulatory Proteins , Antigens, Neoplasm , Artificial Gene Fusion , Gene Fusion , Polymerase Chain Reaction , ... Artificial Gene Fusion , Cloning, Molecular , Gene Expression , Genetic Vectors , Hepatitis B Surface Antigens , Genetics , ... Artificial Gene Fusion , Methods , Carcinoma , Cell Line, Tumor , Genes, Transgenic, Suicide , Humans , Nasopharyngeal ... Humans , Artificial Gene Fusion/methods , Recombinant Fusion Proteins , Escherichia coli/genetics , Cloning, Molecular ...
These fusion linkers code for a 15 amino acid glycine and serine polypeptide, which is an ideal linker peptide for fusion ... Artificial gene synthesis, or gene synthesis, refers to a group of methods that are used in synthetic biology to construct and ... Increasingly, genes are ordered in sets including functionally related genes or multiple sequence variants on a single gene. ... Because artificial gene synthesis does not require template DNA, it is theoretically possible to make a completely synthetic ...
... artificial gene fusion MeSH E05.393.220.870 - two-hybrid system techniques MeSH E05.393.281.526 - genetic complementation test ... artificial, heterologous MeSH E05.820.800.937.525 - insemination, artificial, homologous MeSH E05.820.800.968 - oocyte donation ... cell fusion MeSH E05.200.500.363 - cell separation MeSH E05.200.500.363.285 - cytapheresis MeSH E05.200.500.363.285.570 - ... gene therapy MeSH E05.393.420.451 - genetic enhancement MeSH E05.393.420.601 - protein engineering MeSH E05.393.420.601.035 - ...
However, native enzymes and artificial nanomaterials usually suffer from instability and high cost, which put constraints on ... BCR gene, ABL gene, BCR/ABL fusion gene as well as the mixture of BCR gene, ABL gene and BCR/ABL fusion gene. As depicted in ... BCR gene (image and bar d), BCR/ABL fusion gene (image and bar e), and the mixture of ABL gene, BCR gene, and BCR/ABL fusion ... Determination of Target Fusion Gene. For target fusion gene measurement, 5 μL of 1 μM DNA nanotweezer and 2 μL of 10 μM ...
... compared to an optimized HSV-TK vector in which a hypersensitive mutant of the herpes simplex virus thymidine kinase gene (TK39 ... Improved artificial death switches based on caspases and FADD.. *L Fan, K W Freeman, T Khan, E Pham, D M Spencer ... Kinetics of cell death in T lymphocytes genetically modified with two novel suicide fusion genes. @article{Junker2003KineticsOC ... title={Kinetics of cell death in T lymphocytes genetically modified with two novel suicide fusion genes}, author={K. W. Junker ...
We first designed a FISH-based "fusion assay" for EML4 and ALK genes. Bacterial artificial chromosome fragments encompassing ... Mitelman F, Johansson B, Mertens F. Fusion genes and rearranged genes as a linear function of chromosome aberrations in cancer ... C, fusions between exons of EML4 and ALK. Fusion of exons 6, 13, or 20 of EML4 to exon 20 of ALK gives rise to variants 3, 1, ... Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. Science 2005;310:644-8. ...
fusion protein. A protein made from the natural or artificial hybridization and translation of two distinct genes. ... Mutations in the gene for APP on chromosome 21 account for less than 5% of early-onset familial Alzheimers disease. ... A protein that stimulates the expression of a gene.. acute phase protein. Any of the plasma proteins whose concentration ... The C2824 and H63D mutations on chromosome 6 in the HFE gene cause hemochromatosis. ...
... are ideal for expression studies of the complex genes and gene clusters found in the mammalian genome. Such studies... ... The large regions of DNA that can be cloned in yeast artificial chromosomes (YACs) ... The human HPRT gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusion. Genomics 9, ... functional genes from the HLA class II region into mammalian cells by fusion with yeast spheroplasts carrying an artificial ...
  • However, until about a decade ago, the large size of BACs has presented major hurdle for their precise manipulation to introduce specific changes such as mutations, reporter genes, and markers for functional studies in the mammalian environment [ 11 - 13 ]. (pubmedcentralcanada.ca)
  • Many fusions resulting from chromosomal rearrangements are driver mutations in tumors and are currently used as biomarkers or drug targets. (hindawi.com)
  • For example, in a study in mice, researchers at the Baylor College of Medicine in Houston, Texas, had averted progression of Angelman syndrome, caused by mutations on chromosome 15, by silencing a particular lncRNA - to unleash expression of a gene that it represses. (technologynetworks.com)
  • Mutations of the KRAS and NRAS genes and inactivation of the p53 and RB genes have been demonstrated in a large number of NSCLCs ( 2 - 4 ). (pnas.org)
  • Here we report the identification of two genes, SIN4 and RGR1 , in which recessive mutations cause constitutive MAL gene expression. (genetics.org)
  • Mutations in the gene for APP on chromosome 21 account for less than 5% of early-onset familial Alzheimer disease. (tabers.com)
  • These mechanisms are well suited for introducing precise mutations or knocking in an epitope tag or reporter gene, such as green fluorescent protein. (asnjournals.org)
  • The study sought to determine the incidence of rearrangements on the EWSR1 gene in mesothelioma patients, since previous mutations of the gene have been found in older peritoneal mesothelioma patients. (mesothelioma.com)
  • The researchers also screened for the BAP1 gene when possible, since earlier studies found that the presence of this gene often indicated earlier development of mesothelioma and longer survival than patients who didn't exhibit the gene mutations. (mesothelioma.com)
  • Until recently, chromosomal translocations and fusion genes have been an underappreciated class of mutations in solid tumors. (beds.ac.uk)
  • Runaway telomere elongation caused by telomerase RNA gene mutations", Nature 376, 403-409, 1995. (patentgenius.com)
  • This study presents a comparison of the IFN-antagonist activities of C and V proteins from four attenuated and two wild-type strains of MV. The V proteins were more potent inhibitors of IFN-inducible reporter gene expression than the C proteins, and this effect was unrelated to whether the protein originated from an attenuated or wild-type strain. (nih.gov)
  • Induction of boosted immune response in mice by leptospiral surface proteins expressed in fusion with DnaK. (nih.gov)
  • The recombinant proteins were expressed alone or in fusion with DnaK at the N-terminus. (nih.gov)
  • For statistical analysis, concentration values for the recombinant fusion proteins-immunized group stimulated with the same protein were compared with those immunized and stimulated with the corresponding protein alone by the two-tailed t-test. (nih.gov)
  • A booster effect was elicited by the presence of DnaK in the protein fusions, being statistically significant with the proteins Lsa21, rLIC10494, and C-terminus of Lp95. (nih.gov)
  • All reported partner genes fused in-frame to MLL and the fusion cDNA encode chimeric MLL proteins with a significant portion derived from the partner genes. (aacrjournals.org)
  • All partner genes are fused in-frame to MLL , resulting in novel fusion proteins, rather than simply a truncation of MLL . (aacrjournals.org)
  • The gene for another protein tyrosine kinase, ALK, is fused to those for NPM1 or other partner proteins in anaplastic lymphoma and soft tissue tumors, resulting in an increase in the kinase activity of ALK ( 3 ). (aacrjournals.org)
  • The shared percentage usually refers only to genes that encode instructions for making proteins - while overlooking regulatory genes, which nonetheless make up a large part of the genome. (technologynetworks.com)
  • By comparing "gene neighborhoods" in the vicinity of lncRNAs and of genes that had stopped coding for proteins, the researchers revealed that indeed, about 60 lncRNA genes in mammals - or 2% to 3% of lncRNAs shared by humans and other mammalian species - appear to be derived from ancestral genes. (technologynetworks.com)
  • ONCOGENE FUSION includes an ONCOGENE as at least one of the fusion partners and such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS. (sickkids.ca)
  • Our findings suggest that physical interaction between these two virus proteins might be a prerequisite for primary envelopment of PrV at the inner nuclear membrane and that this envelope is removed by fusion with the outer nuclear membrane. (asm.org)
  • Herpesvirus particles are composed of an icosahedral capsid which encloses the double-stranded DNA genome and which in turn is surrounded by a layer of numerous viral gene products named tegument and a lipid membrane of cellular origin containing mostly glycosylated virus-encoded proteins ( 36 , 43 ). (asm.org)
  • Genes encoding extracellular matrix, junctional, cytoskeletal, and signaling proteins were prominent among those distinguishing the two epithelial cell types. (g3journal.org)
  • Because EHE have either a WWTR1-CAMTA1 (90%) or YAP-TFE3 (10%) gene fusion as the only consistent genetic findings, we propose that all cases of EHE are 'addicted' (see blue sidebar below) to the proteins produced from these gene fusions. (sarcomahelp.org)
  • Some tRFs, akin to microRNAs (miRNAs), are bound by Argonaute (AGO) proteins, suggesting that they may use an miRNA-like mechanism to regulate gene expression ( 4 ). (sciencemag.org)
  • A method for identifying genes within yeast artificial chromosomes: application to isolation of MLL fusion cDNAs from acute leukaemia translocations. (ox.ac.uk)
  • The use of yeast artificial chromosomes (YACs) containing long stretches of human DNA allows dispersed DNA markers to be used to identify the position of breakpoints but does not readily allow subcloning of the precise breakpoint within the YAC DNA nor the cDNAs containing the affected genes. (ox.ac.uk)
  • Bacterial artificial chromosomes, or BACs, are fertility- (F-) factor-based plasmid vectors that replicate stably in low copy number [ 2 , 3 ]. (pubmedcentralcanada.ca)
  • More recently, artificial gene synthesis methods have been developed that will allow the assembly of entire chromosomes and genomes. (wikipedia.org)
  • The large regions of DNA that can be cloned in yeast artificial chromosomes (YACs) are ideal for expression studies of the complex genes and gene clusters found in the mammalian genome. (springer.com)
  • Riley, J. H., Morten, J. E. N., and Anand, R. (1992) Targeted integration of neomycin into yeast artificial chromosomes (YACs) for transfection into mammalian cells. (springer.com)
  • 1993) New vector for transfer of yeast artificial chromosomes to mammalian cells. (springer.com)
  • Isolation of single-copy human genes from a library of yeast artificial chromosomes", Science 244:1348-1351 (1989). (freepatentsonline.com)
  • In particular, spheroplast fusion provides a method for the transfer of intact yeast artificial chromosomes (YACs) into cultured cells ( 11 ). (pnas.org)
  • We have developed a powerful new tool for the physical analysis of genomes called Ty 1 -mediated chromosomal fragmentation and have used the method to map 24 retrotransposon insertions into two different mousederived yeast artificial chromosomes (YACs). (genetics.org)
  • A translocation is a fusion of two chromosomes that are not usually fused. (sarcomahelp.org)
  • Transfer Of Yeast Artificial Chromosomes Into Mammalian Cells And Comparative Study Of Their Integrity," Gene 163:27-33 (1995). (patentgenius.com)
  • Huxley, "Mammalian Artificial Chromosomes: A New Tool For Gene Therapy," Gene Therapy 1:7-12 (1994). (patentgenius.com)
  • Use Of Yeast Artificial Chromosomes (YACs) For Studying Control Of Gene Expression: Correct Regulation Of The Genes Of a Human .beta. (patentgenius.com)
  • Targeted Integration Of Neomycin Into Yeast Artifical Chromosomes (YACs) For Transfection Into Mammalian Cells," Nucleic Acids Research 20(12):2971-2976 (1992). (patentgenius.com)
  • Engineering a Mini-herpesvirus As a General Strategy To Transduce Up To 180 kb Of Functional Self-replicating Human Mini-chromosomes," Gene Therapy 3:1081-1088 (1996). (patentgenius.com)
  • Huxley, "Mammalian Artificial Chromosomes and Chromosome Transgenics," Trends Genet. (patentgenius.com)
  • Arabidopsis IRT2 gene encodes a root-periphery iron transporter. (nih.gov)
  • The ABG1 gene of C. albicans encodes an essential protein located in the vacuolar membranes of both yeast and hyphae. (elsevier.com)
  • To make a gene that encodes a fusion protein, one protein's DNA-with the stop codon deleted-is attached, or "fused," with the DNA of another protein. (reviewofophthalmology.com)
  • 10 The t(8;13) translocation associated with a chronic myeloproliferative disorder, lymphoblastic lymphoma, and eosinophilia juxtaposes the gene for FGFR1 with a novel gene that encodes the zinc finger protein, ZNF198 . (bloodjournal.org)
  • The Novel Gene CRNDE Encodes a Nuclear Peptide (CRNDEP) Which Is Overexpressed in Highly Proliferating Tissues. (sigmaaldrich.com)
  • The resultant TCRβ-Notch1 fusion gene encodes a series of truncated t(7;9)-specific Notch1 polypeptides that localize to the nucleus and structurally resemble ICN1 ( 2 ). (asm.org)
  • The yeast ENA1/PMR2A gene encodes a cation extrusion ATPase in Saccharomyces cerevisiae which is essential for survival under salt stress conditions. (asm.org)
  • Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively. (wikipedia.org)
  • Humans and fish, for instance, share about 70% of their protein-coding genes, but only about 0.5% of an important class of regulatory genes - ones that give rise to so-called long non-coding RNAs, or lncRNAs," says Dr. Igor Ulitsky of the Biological Regulation Department at the Weizmann Institute of Science. (technologynetworks.com)
  • The lncRNAs (pronounced link-RNAs) until recently received much less attention than the protein-coding genes, but they are now proving to be of increasing interest to science. (technologynetworks.com)
  • Identifying such "fossils" of protein-coding genes in the mammalian genome will facilitate further study of human lncRNAs and may ultimately help scientists understand what happens when their function is disrupted. (technologynetworks.com)
  • Gene Fusion" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (sickkids.ca)
  • Escherichia coli responds to the intracellular increase in reactive oxygen species by inducing sets of genes whose products either avert or repair the eventual oxidative damage ( 19 ). (asm.org)
  • A critical phosphate concentration in the stationary phase maintains ndh gene expression and aerobic respiratory chain activity in Escherichia coli. (labome.org)
  • The ASPSCR1-TFE3 fusion gene generated by chromosomal translocation t(X;17)(p11.2;q25) is causally associated with ASPS with the resultant protein modulating its target genes as an oncogenic transcription factor via a DNA-binding motif derived from TFE3 ( 4-7 ). (aacrjournals.org)
  • The study of adaptation mechanisms during salinity stress in the yeast Saccharomyces cerevisiae has revealed several components of sensing and signal transduction pathways, as well as target genes whose expression is activated upon salt stress (for review see references 18 , 45 , and 46 ). (asm.org)
  • Fig. 1 Rhizobial tRFs and their putative target genes in soybean. (sciencemag.org)
  • Unlike other tyrosine kinase receptors activated by N-terminal truncation in tumors, this receptor, FGFR2-ROS, contains an altered C terminus generated from chromosomal rearrangement with a novel gene, designated FGFR activating gene 1 (FRAG1). (pnas.org)
  • Gene expression from bacterial artificial chromosome (BAC) clones has been demonstrated to facilitate physiologically relevant levels compared to viral and nonviral cDNA vectors. (pubmedcentralcanada.ca)
  • Recombineering technology has gradually evolved into a powerful new approach to studying genetic function using genes in their natural genomic context that were widely available from clones in BAC libraries [ 15 ]. (pubmedcentralcanada.ca)
  • In this size range it is necessary to test several candidate clones confirming the sequence of the cloned synthetic gene by automated sequencing methods. (wikipedia.org)
  • Positive clones containing this gene were double digested with NcoI and-BamHI and also approved by sequencing. (bvsalud.org)
  • Three yeast artificial chromosome (YAC) clones spanning the minimal loss of heterozygosity region were modified, and spheroplast fusion was used to transfer them into human A549 NSCLC or murine Lewis lung carcinoma (LLC) cell lines. (pnas.org)
  • The cellular cDNA- neo fusion junction sequences of clones pJET1 to pJET7 were determined. (sciencemag.org)
  • Bioinformatics analysis of sequence reads from early shotgun genome sequencing projects, where clone libraries were constructed and maintained in E. coli , suggests that such IS-mediated inactivation of ectopic genes inhibiting the growth of the E. coli cloning host might happen more frequently than generally anticipated, leading to genomic instability and selection of altered clones. (biomedcentral.com)
  • Moreover, in biotechnological applications, IS-mediated genomic rearrangements can cause instability of clones carrying engineered genes. (biomedcentral.com)
  • The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses. (bioportfolio.com)
  • The MLAA34 and the HSP70 genes were extracted from U937 cells by RT-PCR, and then we amplified the fusion gene MLAA34-HSP70 by SOE-PCR and inserted it into the pIRES2-EGFP vector to construct the pIRES2-MLAA34-HSP70 recombinant vector. (bvsalud.org)
  • We amplified the fusion gene MLAA34-HSP70 successfully and identified the correctness of pIRES2-MLAA34-HSP70 recombinant vector by PCR and restriction endonuclease. (bvsalud.org)
  • Conclusion: In addition to high level expression of the designed gene in this work, specific amino acid sequence of bacterial beta-galactosidase was selected as major part of carrier tag for protection of this hormone as important step of recombinant rhPTH with relevant isoelectronic point [pI]. (bvsalud.org)
  • To investigate if AcsR directly regulates acsA expression, in vitro-gel shift assays were performed using the recombinant AcsR and the regulatory region of the acsA gene, showing that AcsR specifically bound the upstream region of the acsA ORF. (yonsei.ac.kr)
  • Therefore, the main aim of this work was to optimize the temperature, dimethylsulfoxide (DMSO) concentration and the methanol flow-rate for the biosynthesis of recombinant MBCOMT by Pichia pastoris bioreactor methanol-induced cultures using artificial neural networks (ANN). (biomedcentral.com)
  • The latest leap in the evolution of recombinant technology is fusion protein synthesis. (reviewofophthalmology.com)
  • The level and distribution of translating ribosomes on the messenger has an effect on messenger stability, which is tuned partly by the nature of the ribosome binding site and partly by the codon composition of the coding sequence of a gene. (ku.dk)
  • A mouse bacterial artificial chromosome (BAC) containing the entire Npas1 gene, was modified by inserting Cre-2A (cleavable fusion peptide sequence)-tdTomato and polyadenylation sequence from bovine growth hormone downstream of the ATG translation initiation codon, and removing Npas1 coding sequence. (jax.org)
  • It was further shown that Rho terminates RNA polymerases during transcription on messengers normally considered stable, suggesting Rho transcriptional pre-termination to be a global phenomena in gene regulation. (ku.dk)
  • Additionally, the Swi/Snf chromatin-remodeling complex is required for full induction, suggesting a role for chromatin remodeling in the regulation of MAL gene expression. (genetics.org)
  • The role of the Mediator in MAL gene regulation is discussed. (genetics.org)
  • Because Sin4p and Rgr1p are both components of the Sin4 module of the yeast Mediator complex, we compared the roles of the other components of the Sin4 module in MAL gene regulation and found that each plays a distinct role in basal and induced expression. (genetics.org)
  • The interplay between the Mediator and the Swi/Snf complex as it relates to MAL gene regulation is explored. (genetics.org)
  • Although a great number of genes have been found to need HOG signalling for their osmotic up-regulation, the mechanism of gene activation through phosphorylated Hog1p kinase is still unknown. (asm.org)
  • Thus, m(6)A modification adds a new layer of post-transcriptional gene expression regulation, and it is implicated in T cell response to HIV infection, type I interferon production, and T cell differentiation and homeostasis. (readbyqxmd.com)
  • Here, we introduce these artificial riboswitches for regulation of DNA and RNA viruses. (uni-konstanz.de)
  • Importantly, we also demonstrate aptazyme-dependent regulation of measles virus fusion protein expression, translating into potent reduction of progeny infectivity and virus spread. (uni-konstanz.de)
  • Our study implies that gene regulation by artificial riboswitches may be an appealing alternative to Tet- and other protein-dependent gene regulation systems, based on their small size, RNA-intrinsic mode of action, and flexibility of the inducing molecule. (uni-konstanz.de)
  • One of these is recombineering (recombination-mediated genetic engineering [ 14 ]), which was adapted from bacteriophage where the recombination genes were carefully delineated and moved to mobile plasmid systems that were transferable to host E. coli strains. (pubmedcentralcanada.ca)
  • Such T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs), as circularized DNA elements, are formed during the fusion process that creates T- and B-cell receptors [ 7 , 8 ]. (hindawi.com)
  • The GENETIC RECOMBINATION of the parts of two or more GENES resulting in a gene with different or additional regulatory regions, or a new chimeric gene product. (sickkids.ca)
  • The random recombination of the antibody's VH and VL genes also increases the antibody diversity. (selfgrowth.com)
  • In the phage antibody library technology, the random recombination of VH and VL genes mimics the process of in vivo antibody affinity maturation. (selfgrowth.com)
  • Homologous recombination into sites flanking Nf-1 was used to introduce artificial sequences (triple-helix, loxP, vector backbone) that can be employed for in vitro recovery of intervening sequences or the generation of in vivo deletions. (nature.com)
  • Two consecutive homologous recombination events introduced the desired artificial sequences at markers D11Bhm100 and D11Bhm109, flanking the Nf-1 gene at either end ( Figure 1a ). (nature.com)
  • The patient's clinical presentation is distinct from the myeloproliferative/lymphoblastic disorder and the M4/M5 leukemia with erythrophagocytosis previously associated with 8p11 abnormalities, and the fusion event creates a chimeric gene from the potential histone transacetylase, MOZ, and the transcriptional coactivator, TIF2 . (bloodjournal.org)
  • In an earlier study [ 24 ], it was shown that a chimeric gene, constructed for vaccine development, and composed of a synthetic gene of the structural capsid protein VP60 of rabbit haemorrhagic disease (RHD) virus [ 25 ] fused to the gene of the B subunit of cholera toxin (CTX) was very unstable in conventional E. coli hosts. (biomedcentral.com)
  • Genes on transferred YACs are expressed under the control of their normal promoters. (pnas.org)
  • Because the minimal region of LOH on 11q23 in NSCLCs is completely spanned by a YAC contig ( 12 ), we transferred candidate YACs of up to 1.6 Mb and their defined fragmentation derivatives into A549 cells to further localize the region containing a gene(s) that will suppress A549 tumorigenicity in nude mice. (pnas.org)
  • More than 20 different partner genes with MLL have been cloned from leukemia cells with translocations involving chromosome 11 band q23 (11q23). (aacrjournals.org)
  • Unlike DNA synthesis in living cells, artificial gene synthesis does not require template DNA, allowing virtually any DNA sequence to be synthesized in the laboratory. (wikipedia.org)
  • This is usually used when you want just a short sequence from a single gene that you want to use for something such as transfecting into cells in vitro. (physicsforums.com)
  • Optimized fusion gene was synthesized and ligated into pET-28a vector under control of T7 promoter, and then transformed in E. coli [DH5 alpha] cells. (bvsalud.org)
  • An improved bicistronic CD20/tCD34 vector for efficient purification and in vivo depletion of gene-modified T cells for adoptive immunotherapy. (semanticscholar.org)
  • CD34 splice variant: an attractive marker for selection of gene-modified cells. (semanticscholar.org)
  • Genomic rearrangements responsible for the fusion events in NSCLC cells were confirmed by genomic PCR analysis and fluorescence in situ hybridization. (aacrjournals.org)
  • Because both EML4 and ALK genes are mapped to the short arm of chromosome 2 in opposite orientations, PCR with primer sets flanking the fusion points of the two genes would not produce any specific products from cells without inv(2)(p21p23). (aacrjournals.org)
  • Consistent with this notion, recurrent chromosome rearrangements involving genes for ETS transcriptional factors have been identified in many cases of prostate cancer and may contribute to the hypersensitivity of prostate cancer cells to androgens ( 6 , 7 ). (aacrjournals.org)
  • Huxley, C., Hagino, Y., Schlessinger, D., and Olson, M. V. (1991) The human HPRT gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusion. (springer.com)
  • Demmer, L. A. and Chaplin, D. D. (1993) Simultaneous transfer of four functional genes from the HLA class II region into mammalian cells by fusion with yeast spheroplasts carrying an artificial chromosome. (springer.com)
  • Featherstone, T. and Huxley, C. (1993) Extrachromosomal maintenance and amplification of yeast artificial chromosome DNA in mouse cells. (springer.com)
  • We use this system to analyse the progression of viral spread in mouse lungs, for live imaging of virus-infected cells, and for differential gene expression studies in virus antigen-positive and virus antigen-negative live cells in the lungs of Color-flu-infected mice. (springernature.com)
  • Procedures that introduce large segments of DNA into cells can be used to localize genes for which a functional assay is available ( 10 ). (pnas.org)
  • Ty 1 fusion element marked with the retrotransposition indicator gene, ade2AI , resulted in a high fraction of cells that sustained a single Ty 1 insertion marked with ADE2 . (genetics.org)
  • This selection yielded six independent chromosomal lacZ transcriptional fusions that were activated by either artificial expression of SoxS or exposure of wild-type cells to micromolar concentrations of paraquat. (asm.org)
  • After its artificial overexpression in HeLa cells, in a fusion with either the EGFP or DsRed Monomer fluorescent tag, CRNDEP seems to stimulate the formation of stress granules and localize to them. (sigmaaldrich.com)
  • Translation of the fusion mRNA results in the production of a functional protein that can transform G418-sensitive (G418 S ) cells to G418 resistance (G418 R ). The lowercase letters in the intron represent the sequence of the mneoIRF1 artificial splice acceptor site. (sciencemag.org)
  • The principle of phage antibody library technology is to use PCR technology to amplify a complete set of antibody heavy chain variable region (VH) and light chain variable region (VL) genes from human immune cells, and clone them into a phage vector, which is then expressed on the surface of its shell in the form of a fusion protein. (selfgrowth.com)
  • To define the similarities and distinctions between periderm and basal skin epithelial cells, we purified them from zebrafish at early development stages and deeply profiled their gene expression. (g3journal.org)
  • At each of 52 and 72 hr post-fertilization (hpf), more than 60% of genes enriched in skin cells were similarly expressed in both layers, indicating that they were common epithelial genes, but many others were enriched in one layer or the other. (g3journal.org)
  • The second aim was to determine the molecular mechanisms that cause the WWTR1-CAMTA1 fusion protein to transform EHE precursor cells. (sarcomahelp.org)
  • We applied paired-end RNA-seq to identify 24 novel and 3 previously known fusion genes in breast cancer cells. (beds.ac.uk)
  • In summary, using RNA-sequencing and improved bioinformatic stratification, we have discovered a number of novel fusion genes in breast cancer, and identified VAPB-IKZF3 as a potential fusion gene with importance for the growth and survival of breast cancer cells. (beds.ac.uk)
  • YAC Transfer Into Mammalian Cells By Cell Fusion," Methods in Molecular Biology 54:281-292 (1996). (patentgenius.com)
  • The Human HPRT Gene On a Yeast Artificial Chromosome Is Functional When Transferred To Mouse Cells By Cell Fusion," Genomics 9:742-750 (1991). (patentgenius.com)
  • A putative histidine kinase gene, acsS, was located five ORFs downstream of the acsR gene. (yonsei.ac.kr)
  • Complementation analysis was used to further localize a putative tumor suppressor gene. (pnas.org)
  • Nucleotide sequence determination identified the disrupted genes as sodA (Mn-containing superoxide dismutase), fpr (NADPH:ferredoxin oxidoreductase), and ydbK (a putative Fe-S-containing reductase). (asm.org)
  • This is likely a phenomenon that occurs in many types of cancers when oncogenic fusion genes are over-expressed," says the study's senior author, Dr. Mark A. Rubin, The Homer T. Hirst Professor of Oncology in Pathology and vice chair for experimental pathology at Weill Cornell Medical College. (freedomsphoenix.com)
  • However, only a fraction of gene fusions predicted based on DNA-seq is expected to generate an expressed fusion mRNA, making this approach tedious to discover activated, oncogenic fusion gene events. (beds.ac.uk)
  • The MOZ - TIF2 fusion is one of a new family of chromosomal rearrangements that associate HAT activity, transcriptional coactivation, and acute leukemia. (bloodjournal.org)
  • Shago M, Abla O, Hitzler J, Weitzman S, Abdelhaleem M. Frequency and outcome of pediatric acute lymphoblastic leukemia with ZNF384 gene rearrangements including a novel translocation resulting in an ARID1B/ZNF384 gene fusion. (sickkids.ca)
  • Next-generation sequencing technologies provide an opportunity for systematic characterization of cancer cell transcriptomes, including the discovery of expressed fusion genes resulting from underlying genomic rearrangements. (beds.ac.uk)
  • Whole-genome DNA-sequencing (DNA-seq) can also be used to identify potential fusion-gene-creating rearrangements. (beds.ac.uk)
  • Alternative splicing of human gephyrin gene created two different forms of fusion cDNA. (aacrjournals.org)
  • The exogenous genes for library construction come from mmDNA extracted from human peripheral blood, bone marrow, or lymphocytes of the spleen, which is reversely transcribed to form cDNA. (selfgrowth.com)
  • Recurrent NTRK1 Gene Fusions Define a Novel Subset of Locally Aggressive Lipofibromatosis-like Neural Tumors. (sickkids.ca)
  • The human Notch1 gene was originally identified by analysis of a recurrent (7;9) chromosomal translocation [t(7;9)] found in a subset of T-cell lymphoblastic leukemias and lymphomas (T-ALL) ( 9 ). (asm.org)
  • The recent identification of recurrent ETS -family translocations in prostate cancer [ 3 ] and EML4-ALK in lung cancer [ 4 ] now suggests that fusion genes may play an important role also in the development of epithelial cancers. (beds.ac.uk)
  • However, using conventional methods of gene inactivation in the mouse, deletions in the megabase range are still difficult to reproduce. (nature.com)
  • Dr. Rubin adds that if such an oncogenic protein has the power to throw the switch on thousands of genes, a novel treatment may be able to turn that switch off. (freedomsphoenix.com)
  • Such deletions range from small intragenic deletions to loss of large chromosomal regions encompassing thousands of genes. (nature.com)
  • Borel F, Mueller C. Design, Cloning, and In Vitro Screening of Artificial miRNAs to Silence Alpha-1 Antitrypsin. (umassmed.edu)
  • We show by fluorescence in situ hybridization (FISH) analysis, Southern blotting, and reverse transcriptase-polymerase chain reaction (RT-PCR) that the genes for MOZ , mo nocytic leukemia z inc finger protein, and TIF2 , t ranscriptional i ntermediary f actor 2, are involved in the inv(8)(p11q13). (bloodjournal.org)
  • We validate the fusion events and demonstrate their potential biological significance by RT-PCR, fluorescence in situ hybridization (FISH) and RNA interference (RNAi), thereby highlighting the importance of gene fusions in breast cancer. (beds.ac.uk)
  • His team members developed a series of algorithms that enabled them to find such "recycled" genes in the mammalian genome. (technologynetworks.com)
  • The information generated from the Genome Projects will be of the greatest value if it can be converted into functional data, particularly if this increases our understanding of normal gene function and allows strategies to be developed for prevention and treatment of human disease. (pubmedcentralcanada.ca)
  • Thus, BACs carrying full-length genes in their natural chromosomal setting is becoming an attractive resource for studying genome structure and function, representing an exciting alternative to conventional vector systems. (pubmedcentralcanada.ca)
  • Tan said the research team - which also included scientists from the National University of Singapore, National Cancer Centre of Singapore, the Genome Institute of Singapore, Yonsei University College of Medicine in Seoul, South Korea, and Howard University - used a novel genomic approach to isolate the fusion gene. (healthcanal.com)
  • EML4-ALK is a fusion-type protein tyrosine kinase that is generated by inv(2)(p21p23) in the genome of non-small cell lung cancer (NSCLC). (aacrjournals.org)
  • Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents," Proc. (freepatentsonline.com)
  • Not only are there as many as 20,000 lncRNA genes in the human genome - about the same number as the protein-coding ones - but the lncRNAs have lately been revealed to serve as master switches in a wide variety of biological processes. (technologynetworks.com)
  • In a recent study published in the journal Genome Biology, Ulitsky and his team - research students Hadas Hezroni, Gali Housman and Zohar Meir, and staff scientists Drs. Rotem Ben-Tov Perry and Yoav Lubelsky - managed to identify a class of mammalian lncRNAs that had evolved from more ancient genes by taking on new functions. (technologynetworks.com)
  • But the fact that these genes have been conserved in the genome for so long suggests that they play important roles in the cell. (technologynetworks.com)
  • The RovA regulons of Yersinia enterocolitica and Yersinia pestis are distinct: evidence that many RovA-regulated genes were acquired more recently than the core genome. (nih.gov)
  • A 2.6-kbp fragment of the pseudorabies virus (PrV) genome was sequenced and shown to contain the homologues of the highly conserved herpesvirus genes UL31 and UL32. (asm.org)
  • Yeast two-hybrid studies revealed that a UL31 fusion protein specifically interacts with plasmids of a PrV genome library expressing the N-terminal part of UL34. (asm.org)
  • luxAB transcriptional fusion was decreased in both ΔacsR and ΔacsS mutants. (yonsei.ac.kr)
  • To identify additional players involved in regulating MAL gene expression, we carried out a genetic selection for MAL constitutive mutants. (genetics.org)
  • Strain CMY4000 containing MAL1 and integrated copies of MAL61promoter-HIS3 and MAL61promoter-lacZ reporter genes was used to select constitutive mutants. (genetics.org)
  • Group 1 and group 2 mutants exhibit pleiotropic phenotypes and represent alleles of Mediator component genes RGR1 and SIN4 , respectively. (genetics.org)
  • To identify possible additional players involved in regulating MAL gene expression, we designed a sensitive genetic selection for MAL constitutive (Mal c ) mutants using a MAL61promoter-HIS3 reporter. (genetics.org)
  • The cloned soxRS genes were used to generate soxRS deletion mutants by allelic exchange (Martins et al. (asm.org)
  • The tolC mutants carrying multiple copies of the microcin genes were less immune to the microcin. (labome.org)
  • This approach is best suited for generating point mutants or knocking out a gene. (asnjournals.org)
  • Mutants lacking the first ENA gene in the gene cluster ( ena1 ) are hypersensitive to salt stress ( 17 ). (asm.org)
  • and ( b ) partner genes could confer gain-of-function activity. (aacrjournals.org)
  • Fusion partner genes were found to contribute promoters (5' UTR), coding sequences and 3' UTRs. (beds.ac.uk)
  • Some of the fusion partner genes, such as GSDMB in the TATDN1-GSDMB fusion and IKZF3 in the VAPB-IKZF3 fusion, were only detected as a fusion transcript, indicating activation of a dormant gene by the fusion event. (beds.ac.uk)
  • To date, a few oomycete genomes have been sequenced, and this enables a comprehensive comparison of genomic features present in oomycetes, fungi, and other eukaryotic species such as gene families and protein domains. (plantphysiol.org)
  • Gene overexpression was observed in SDS-PAGE after induction with 0.2 mM IPTG. (bvsalud.org)
  • The reciprocal TIF2 - MOZ fusion gene is not expressed, perhaps as a result of a deletion near the chromosome 8 centromere. (bloodjournal.org)
  • Here, we describe a versatile procedure for chromosomal engineering that was used to generate an ES cell line with a megabase deletion encompassing the tumour suppressor gene neurofibromatosis-1 (Nf-1) on mouse chromosome 11, which is often deleted in tumours of neural crest origin. (nature.com)
  • The invention of microarray technology and its ability to simultaneously interrogate thousands genes has changed the paradigm of molecular classification of human cancers as well as it shifted clinical prognosis model to broader prospect. (cscjournals.org)
  • Gene fusion is a molecular event occurring in cellular proliferation and differentiation, and the occurrence of irregular fusion gene results in various malignant diseases. (hindawi.com)
  • Gene fusions are a molecular event in cancer [ 1 - 3 ]. (hindawi.com)
  • Meanwhile, gene fusions are also a necessary molecular event in the defense of cancer and other diseases. (hindawi.com)
  • Molecular mechanism for ganciclovir resistance in human T lymphocytes transduced with retroviral vectors carrying the herpes simplex virus thymidine kinase gene. (semanticscholar.org)
  • By use of a monospecific antiserum, the UL31 gene product was identified as a nuclear protein with an apparent molecular mass of 29 kDa. (asm.org)
  • Analyzing the molecular details, we identify a hydrophobic protein as the toxic byproduct of the clone, and show that IS elements spontaneously landing in the cloned fragment relieve the cell from the stress by blocking transcription of the toxic gene. (biomedcentral.com)
  • The functional significance of this de facto truncated MLL gene product is not clear. (aacrjournals.org)
  • This complementation approach localized tumor suppression activity to the central 700 kb of 939b12 and provides a functional assay for positional cloning of this tumor suppressor gene. (pnas.org)
  • The presence of a tumor suppressor gene(s) on Chr 11q in NSCLCs was also demonstrated by using a functional assay. (pnas.org)
  • Experimentally derived functional knowledge of the majority of gene products in oomycetes in a comparable depth as for model species like Saccharomyces cerevisiae and Arabidopsis ( Arabidopsis thaliana ) will likely not be accessible in the near future. (plantphysiol.org)
  • Functional Reconstitution Of Wild-Type And Mutant Tetrahymena Telomerase," Genes & Development 8:563-575 (1994). (patentgenius.com)
  • In the present study, we have identified human gephyrin as a novel fusion partner of MLL from a patient who presented with a de novo acute monoblastic leukemia with t(11;14)(q23;q24). (aacrjournals.org)
  • We have identified the genes involved in a novel fusion event in a case of acute mixed-lineage leukemia associated with an inv(8)(p11q13). (bloodjournal.org)
  • 95% confirmation rate of the identified fusion events, we identify several novel fusion genes in breast cancer from as little as a single lane of sequencing on an Illumina GA2x instrument. (beds.ac.uk)
  • However, native enzymes and artificial nanomaterials usually suffer from instability and high cost, which put constraints on their further application. (hindawi.com)
  • BACs are large enough to transfer intact genes in their native chromosomal setting together with flanking regulatory elements to provide all the signals for correct spatiotemporal gene expression. (pubmedcentralcanada.ca)
  • Because of their large insert capacity, BACs can carry complete genes containing flanking distant regulatory DNA that provide signals for correct spatio-temporal gene expression [ 4 - 9 ]. (pubmedcentralcanada.ca)
  • The inference of breast cancer metastasis through gene regulatory networks. (cscjournals.org)
  • They turn genes on and off and affect other regulatory genes, controlling cellular fate during fetal development, as well as cellular division and death in the adult organism. (technologynetworks.com)
  • It may one day be possible to treat these disorders by targeting the lncRNAs so as to reprogram entire gene regulatory networks. (technologynetworks.com)
  • To investigate the roles of this regulatory cascade, the target gene regulated by a LuxR-regulator was identified and its expression was characterized. (yonsei.ac.kr)
  • The response to increased levels of superoxide is regulated by the soxRS system, a pair of regulatory genes that, together with their downstream-regulated genes, define the soxRS regulon ( 3 , 37 ). (asm.org)
  • Moreover, the regulatory region between the two genes is nearly identical to that of E. coli . (asm.org)
  • The SET [Su-(var)3-9, enhancer of zeste, and trithorax] domain in the COOH terminus of MLL , along with the plant homeodomain fingers, are conserved with the Drosophila trx 3 gene. (aacrjournals.org)
  • As the Cre-ERT2 fusion gene is under control of the Wfs1 promoter/enhancer regions within the BAC transgene, cre activity is directed to cortex, hippocampus, and cerebellum only following tamoxifen administration. (jax.org)
  • The t(7;9) disrupts the Notch1 gene, fusing the portion encoding its intracellular domain (ICN1) to enhancer and promoter elements of the T-cell receptor β (TCRβ) gene. (asm.org)
  • This is the first report showing the existence of a peptide encoded by the CRNDE gene. (sigmaaldrich.com)
  • In addition, fusion event occurred in cellular development, and proliferation is similar to the "AND" logic gate event in computer science which can be harnessed for intelligent and versatile detection. (hindawi.com)
  • Hence, comparative genomics provides an important framework to functionally characterize oomycete gene products and generate hypotheses on the basic cellular functions as well as the complex interactions of these plant pathogens with their hosts and environment. (plantphysiol.org)
  • The predicted structure of an L1 retrotransposition event into a cellular gene is shown. (sciencemag.org)
  • The fusion mRNA is generated by splicing of the preceding exons of a cellular gene to the artificial splice acceptor in the neo indicator gene. (sciencemag.org)
  • In every instance, the upstream cellular exons spliced to the artificial splice acceptor sequence in the predicted manner. (sciencemag.org)
  • Gene editing can be defined as the process of permanently altering cellular DNA sequences to introduce a desired mutation. (asnjournals.org)
  • Research into cellular repair mechanisms for DNA double-strand breaks (DSBs) underpins newer approaches to gene editing. (asnjournals.org)
  • Increased expression of the ENA1 gene has been found to represent a crucial cellular response after salt challenge. (asm.org)
  • In one product, MLL was fused in-frame to the human gephyrin gene, and the resultant fusion protein contained the NH 2 terminus of MLL and the COOH terminus of the human gephyrin protein. (aacrjournals.org)
  • 2 The resultant fusion protein tightly binds to all isoforms of VEGF-A and both forms of Placental Growth Factor (PlGF1 and 2). (reviewofophthalmology.com)
  • This system was used to demonstrate that soxS expression is sufficient for the induction of resistance to the superoxide-generating drug paraquat and for the transcriptional activation of the sodA and micF genes. (asm.org)
  • Therefore, Ca 2+ /calmodulin signalling may act, at least in part, through the transcriptional activation of ion transporter genes such as ENA1 . (asm.org)
  • Herein, we aimed to verify our hypothesis that the CRNDE gene may encode a protein product, CRNDEP. (sigmaaldrich.com)
  • 1993) Creation of mice expressing human antibody light chains by introduction of a yeast artificial chromosome containing the core region of the human immunoglobulin κ locus. (springer.com)
  • In this way, the presence of antibody genes in the phage DNA and the expression of antibody molecules on the surface of the phage DNA contribute to using antigen-antibody specific binding to screen out the required antibodies for clonal amplification. (selfgrowth.com)
  • By expressing the antibody gene in a secreted manner, soluble antibody fragments can be obtained. (selfgrowth.com)
  • If the antibody mRNA is from an unimmunized person, a human natural antibody library can be established without cell fusion. (selfgrowth.com)
  • 2. Use antibody light chain and heavy chain primers to amplify different Ig gene fragments by PCR technology. (selfgrowth.com)
  • Due to the large capacity and extremely high screening efficiency of the antibody library, it is possible to utilize any antibody gene to prepare antibodies by genetic engineering methods, thereby avoiding the use of artificial immune animals and cell fusion technology. (selfgrowth.com)
  • The antibody genes used are from the human body. (selfgrowth.com)
  • Here, we proposed a nonenzymatic and high-throughput fluorescent assay system for the detection of fusion gene by employing DNA nanotweezers with hydrolytic activity. (hindawi.com)
  • Taking BCR/ABL fusion gene as an example, the tweezer-based assay system showed not only excellent distinguishing capability towards different input targets but also high sensitivity with a detection limit of 5.29 pM. (hindawi.com)
  • Primers were designed to detect all possible in-frame fusions of EML4 to exon 20 of ALK , and a single-tube multiplex RT-PCR assay was done with total RNA from 656 solid tumors of the lung ( n = 364) and 10 other organs. (aacrjournals.org)
  • Although this assay clearly implicates Chr 11, it does not provide for the precise subchromosomal localization of the tumor suppressor gene(s). (pnas.org)
  • The analysis of genes differentially expressed during infection revealed a significant enrichment of genes encoding expanded domains as well as signal peptides linking a substantial part of these genes to pathogenicity. (plantphysiol.org)
  • These analyses identified groups of genes whose tissue enrichment changed at each stage, defining gene flow dynamics of maturing vertebrate epithelia. (g3journal.org)
  • The ENA/PMR2 gene cluster of S. cerevisiae contains a tandem array of nearly identical genes encoding P-type ATPases involved in the extrusion of Na + and Li + ions from the cytoplasm ( 17 , 59 ). (asm.org)
  • Silencing individual tRFs with the use of short tandem target mimics or by overexpressing their targets represses root hair curling and nodule formation, whereas repressing these targets with artificial microRNAs identical to the respective tRFs or mutating these targets with CRISPR-Cas9 promotes nodulation. (sciencemag.org)
  • Usually these segments of a particular gene can only be synthesized by splitting the procedure into several consecutive steps and a final assembly of shorter sub-sequences, which in turn leads to a significant increase in time and labor needed for its production. (wikipedia.org)
  • Here it is shown that L1s insert into transcribed genes and retrotranspose sequences derived from their 3′ flanks to new genomic locations. (sciencemag.org)
  • Thus, retrotransposition-competent L1s provide a vehicle to mobilize non-L1 sequences, such as exons or promoters, into existing genes and may represent a general mechanism for the evolution of new genes. (sciencemag.org)
  • C ) Sequences at the junctions of the fusion cDNAs. (sciencemag.org)
  • It can reveal the absence or presence of DNA sequences, or can be used to study RNA targets to reveal gene expression. (mesothelioma.com)
  • In one form, human gephyrin gene fused in-frame to MLL exon 9, and the chimeric product had COOH terminus of human gephyrin protein, including the tubulin binding site. (aacrjournals.org)
  • We were able to identify two MLL -human gephyrin fusion products, presumably attributable to the alternative splicing of human gephyrin . (aacrjournals.org)
  • Gene expression profiles based Human cancer diseases classification. (cscjournals.org)
  • Pavan, W. J., Hieter, P., and Reeves, R. H. (1990) Modification and transfer into an embryonal carcinoma cell line of a 360-kilobase human-derived yeast artificial chromosome. (springer.com)
  • Loss of heterozygosity on chromosome 11q23 is observed at high frequency in human nonsmall cell lung carcinomas (NSCLCs), suggesting the presence of a tumor suppressor gene. (pnas.org)
  • VEGF-Trap is made from the DNA portions of the principal binding domains of VEGF receptors 1 and 2 (VEGFR1 and VEGFR2) and the gene sequence for human IgG1. (reviewofophthalmology.com)
  • CRNDE, recently described as the lncRNA-coding gene, is overexpressed at RNA level in human malignancies. (sigmaaldrich.com)
  • Finally, we describe prospects for the use of gene editing to treat human genetic diseases. (asnjournals.org)
  • To identify potential toxicities, we consulted the existing evidence from AAV gene therapy for other nervous system disorders (animal toxicology and human studies) and from the clinical pharmacology of conventional analgesic drugs. (biomedcentral.com)
  • In addition to good detection performance, this system was simple and enzyme-free, offering a powerful nanometer tool as a smart nanodevice for sensing fusion detection. (hindawi.com)
  • To allow sensitive detection of EML4-ALK fusion transcripts, we have now developed a multiplex reverse transcription-PCR (RT-PCR) system that captures all in-frame fusions between the two genes. (aacrjournals.org)
  • Here, we demonstrate the effectiveness of paired-end RNA-seq in the comprehensive detection of fusion genes. (beds.ac.uk)
  • These results thus define three new groups, FA-F, FA-G, and FA-H, providing evidence for a minimum of eight distinct FA genes. (nih.gov)
  • Here we set out to define the gene expression programs that determine common and distinguishing features of distinct epithelial cell types as they mature. (g3journal.org)
  • Explains Ulitsky: "In recent years, lncRNAs were found to be important for the activation or repression of genes relevant to a variety of disorders. (technologynetworks.com)
  • This repression can be selectively relieved by regulators such as RovA, and the observation that nearly all RovA-activated genes are repressed by H-NS is consistent with this model. (nih.gov)
  • The technique of ribosomal foot printing and whole transcriptome deep sequencing was employed to facilitate an overview of the changes in gene expression upon induction of the pseudoknot construct. (ku.dk)
  • It was demonstrated that typical genes associated with the heat stress response were upregulated -a response that suggested the pseudoknot construct possibly conferred misfolding of the fusion gene or were unable to release ribosomes for reinitiation in the translation cycle. (ku.dk)
  • Artificial gene synthesis, or gene synthesis, refers to a group of methods that are used in synthetic biology to construct and assemble genes from nucleotides de novo. (wikipedia.org)