A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.
ARTHRITIS that is induced in experimental animals. Immunological methods and infectious agents can be used to develop experimental arthritis models. These methods include injections of stimulators of the immune response, such as an adjuvant (ADJUVANTS, IMMUNOLOGIC) or COLLAGEN.
Arthritis of children, with onset before 16 years of age. The terms juvenile rheumatoid arthritis (JRA) and juvenile idiopathic arthritis (JIA) refer to classification systems for chronic arthritis in children. Only one subtype of juvenile arthritis (polyarticular-onset, rheumatoid factor-positive) clinically resembles adult rheumatoid arthritis and is considered its childhood equivalent.
A type of inflammatory arthritis associated with PSORIASIS, often involving the axial joints and the peripheral terminal interphalangeal joints. It is characterized by the presence of HLA-B27-associated SPONDYLARTHROPATHY, and the absence of rheumatoid factor.
An aseptic, inflammatory arthritis developing secondary to a primary extra-articular infection, most typically of the GASTROINTESTINAL TRACT or UROGENITAL SYSTEM. The initiating trigger pathogens are usually SHIGELLA; SALMONELLA; YERSINIA; CAMPYLOBACTER; or CHLAMYDIA TRACHOMATIS. Reactive arthritis is strongly associated with HLA-B27 ANTIGEN.
The inner membrane of a joint capsule surrounding a freely movable joint. It is loosely attached to the external fibrous capsule and secretes SYNOVIAL FLUID.
Also known as articulations, these are points of connection between the ends of certain separate bones, or where the borders of other bones are juxtaposed.
The clear, viscous fluid secreted by the SYNOVIAL MEMBRANE. It contains mucin, albumin, fat, and mineral salts and serves to lubricate joints.
Arthritis, especially of the great toe, as a result of gout. Acute gouty arthritis often is precipitated by trauma, infection, surgery, etc. The initial attacks are usually monoarticular but later attacks are often polyarticular.
Antibodies found in adult RHEUMATOID ARTHRITIS patients that are directed against GAMMA-CHAIN IMMUNOGLOBULINS.
Inflammation of a synovial membrane. It is usually painful, particularly on motion, and is characterized by a fluctuating swelling due to effusion within a synovial sac. (Dorland, 27th ed)
A progressive, degenerative joint disease, the most common form of arthritis, especially in older persons. The disease is thought to result not from the aging process but from biochemical changes and biomechanical stresses affecting articular cartilage. In the foreign literature it is often called osteoarthrosis deformans.
A fibrillar collagen found predominantly in CARTILAGE and vitreous humor. It consists of three identical alpha1(II) chains.
An antineoplastic antimetabolite with immunosuppressant properties. It is an inhibitor of TETRAHYDROFOLATE DEHYDROGENASE and prevents the formation of tetrahydrofolate, necessary for synthesis of thymidylate, an essential component of DNA.
Levels within a diagnostic group which are established by various measurement criteria applied to the seriousness of a patient's disorder.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
Measurement of rate of settling of erythrocytes in anticoagulated blood.
Methods of delivering drugs into a joint space.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Disorders of connective tissue, especially the joints and related structures, characterized by inflammation, degeneration, or metabolic derangement.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Roentgenography of a joint, usually after injection of either positive or negative contrast medium.
A subspecialty of internal medicine concerned with the study of inflammatory or degenerative processes and metabolic derangement of connective tissue structures which pertain to a variety of musculoskeletal disorders, such as arthritis.
A synovial hinge connection formed between the bones of the FEMUR; TIBIA; and PATELLA.
The articulation between a metacarpal bone and a phalanx.
A chronic inflammatory condition affecting the axial joints, such as the SACROILIAC JOINT and other intervertebral or costovertebral joints. It occurs predominantly in young males and is characterized by pain and stiffness of joints (ANKYLOSIS) with inflammation at tendon insertions.
A drug that is used in the management of inflammatory bowel diseases. Its activity is generally considered to lie in its metabolic breakdown product, 5-aminosalicylic acid (see MESALAMINE) released in the colon. (From Martindale, The Extra Pharmacopoeia, 30th ed, p907)
Peptides whose amino and carboxy ends are linked together with a peptide bond forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS. Some of them are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL).
Disorders that are characterized by the production of antibodies that react with host tissues or immune effector cells that are autoreactive to endogenous peptides.
Antibodies produced by a single clone of cells.
A variable mixture of the mono- and disodium salts of gold thiomalic acid used mainly for its anti-inflammatory action in the treatment of rheumatoid arthritis. It is most effective in active progressive rheumatoid arthritis and of little or no value in the presence of extensive deformities or in the treatment of other forms of arthritis.
The joint that is formed by the distal end of the RADIUS, the articular disc of the distal radioulnar joint, and the proximal row of CARPAL BONES; (SCAPHOID BONE; LUNATE BONE; triquetral bone).
Subcutaneous nodules seen in 20-30% of rheumatoid arthritis patients. They may arise anywhere on the body, but are most frequently found over the bony prominences. The nodules are characterized histologically by dense areas of fibrinoid necrosis with basophilic streaks and granules, surrounded by a palisade of cells, mainly fibroblasts and histiocytes.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
A subtype of HLA-DRB beta chains that includes over one hundred allele variants. The HLA-DRB1 subtype is associated with several of the HLA-DR SEROLOGICAL SUBTYPES.
The articulation between the head of one phalanx and the base of the one distal to it, in each finger.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
The articulations between the various TARSAL BONES. This does not include the ANKLE JOINT which consists of the articulations between the TIBIA; FIBULA; and TALUS.
An infectious disease caused by a spirochete, BORRELIA BURGDORFERI, which is transmitted chiefly by Ixodes dammini (see IXODES) and pacificus ticks in the United States and Ixodes ricinis (see IXODES) in Europe. It is a disease with early and late cutaneous manifestations plus involvement of the nervous system, heart, eye, and joints in variable combinations. The disease was formerly known as Lyme arthritis and first discovered at Old Lyme, Connecticut.
Cell surface receptors that bind TUMOR NECROSIS FACTORS and trigger changes which influence the behavior of cells.
An HLA-DR antigen which is associated with HLA-DRB1 CHAINS encoded by DRB1*04 alleles.
Substances that reduce or suppress INFLAMMATION.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Heterogeneous group of arthritic diseases sharing clinical and radiologic features. They are associated with the HLA-B27 ANTIGEN and some with a triggering infection. Most involve the axial joints in the SPINE, particularly the SACROILIAC JOINT, but can also involve asymmetric peripheral joints. Subsets include ANKYLOSING SPONDYLITIS; REACTIVE ARTHRITIS; PSORIATIC ARTHRITIS; and others.
The articulations extending from the ANKLE distally to the TOES. These include the ANKLE JOINT; TARSAL JOINTS; METATARSOPHALANGEAL JOINT; and TOE JOINT.
A common genetically determined, chronic, inflammatory skin disease characterized by rounded erythematous, dry, scaling patches. The lesions have a predilection for nails, scalp, genitalia, extensor surfaces, and the lumbosacral region. Accelerated epidermopoiesis is considered to be the fundamental pathologic feature in psoriasis.
A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.
The joint that is formed by the inferior articular and malleolar articular surfaces of the TIBIA; the malleolar articular surface of the FIBULA; and the medial malleolar, lateral malleolar, and superior surfaces of the TALUS.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A proinflammatory cytokine produced primarily by T-LYMPHOCYTES or their precursors. Several subtypes of interleukin-17 have been identified, each of which is a product of a unique gene.
Abnormal fluid accumulation in TISSUES or body cavities. Most cases of edema are present under the SKIN in SUBCUTANEOUS TISSUE.
An antigen solution emulsified in mineral oil. The complete form is made up of killed, dried mycobacteria, usually M. tuberculosis, suspended in the oil phase. It is effective in stimulating cell-mediated immunity (IMMUNITY, CELLULAR) and potentiates the production of certain IMMUNOGLOBULINS in some animals. The incomplete form does not contain mycobacteria.
The region in the hindlimb of a quadruped, corresponding to the human ANKLE.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Inflammation of the synovial lining of a tendon sheath. Causes include trauma, tendon stress, bacterial disease (gonorrhea, tuberculosis), rheumatic disease, and gout. Common sites are the hand, wrist, shoulder capsule, hip capsule, hamstring muscles, and Achilles tendon. The tendon sheaths become inflamed and painful, and accumulate fluid. Joint mobility is usually reduced.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*27 allele family.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Organic compounds that contain GOLD as an integral part of the molecule. Some are used as ANTIRHEUMATIC AGENTS. The term chrysotherapy derives from an ancient Greek term for gold.
Therapy with two or more separate preparations given for a combined effect.
The articulations extending from the WRIST distally to the FINGERS. These include the WRIST JOINT; CARPAL JOINTS; METACARPOPHALANGEAL JOINT; and FINGER JOINT.
An aldose-ketose isomerase that catalyzes the reversible interconversion of glucose 6-phosphate and fructose 6-phosphate. In prokaryotic and eukaryotic organisms it plays an essential role in glycolytic and gluconeogenic pathways. In mammalian systems the enzyme is found in the cytoplasm and as a secreted protein. This secreted form of glucose-6-phosphate isomerase has been referred to as autocrine motility factor or neuroleukin, and acts as a cytokine which binds to the AUTOCRINE MOTILITY FACTOR RECEPTOR. Deficiency of the enzyme in humans is an autosomal recessive trait, which results in CONGENITAL NONSPHEROCYTIC HEMOLYTIC ANEMIA.
Determination of the degree of a physical, mental, or emotional handicap. The diagnosis is applied to legal qualification for benefits and income under disability insurance and to eligibility for Social Security and workmen's compensation benefits.
An unpleasant sensation induced by noxious stimuli which are detected by NERVE ENDINGS of NOCICEPTIVE NEURONS.
A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
Diseases which have one or more of the following characteristics: they are permanent, leave residual disability, are caused by nonreversible pathological alteration, require special training of the patient for rehabilitation, or may be expected to require a long period of supervision, observation, or care. (Dictionary of Health Services Management, 2d ed)
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
A latent susceptibility to disease at the genetic level, which may be activated under certain conditions.
A ligand that binds to but fails to activate the INTERLEUKIN 1 RECEPTOR. It plays an inhibitory role in the regulation of INFLAMMATION and FEVER. Several isoforms of the protein exist due to multiple ALTERNATIVE SPLICING of its mRNA.
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
Component of the NATIONAL INSTITUTES OF HEALTH. It supports research into the causes, treatment, and prevention of arthritis and musculoskeletal and skin diseases; the training of basic and clinical scientists to carry out this research; and the dissemination of information on research progress. It was established in 1986.
Elements of limited time intervals, contributing to particular results or situations.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
Azoles with an OXYGEN and a NITROGEN next to each other at the 1,2 positions, in contrast to OXAZOLES that have nitrogens at the 1,3 positions.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
A glucocorticoid with the general properties of the corticosteroids. It is the drug of choice for all conditions in which routine systemic corticosteroid therapy is indicated, except adrenal deficiency states.
3-Mercapto-D-valine. The most characteristic degradation product of the penicillin antibiotics. It is used as an antirheumatic and as a chelating agent in Wilson's disease.
A specific species of bacteria, part of the BORRELIA BURGDORFERI GROUP, whose common name is Lyme disease spirochete.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)
Infections with bacteria of the genus YERSINIA.
Pain in the joint.
Inflammation of the joints of the SPINE, the intervertebral articulations.
Process whereby the immune system reacts against the body's own tissues. Autoimmunity may produce or be caused by AUTOIMMUNE DISEASES.
Scales, questionnaires, tests, and other methods used to assess pain severity and duration in patients or experimental animals to aid in diagnosis, therapy, and physiological studies.
The articulation between the head of one phalanx and the base of the one distal to it, in each toe.
Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A large multinuclear cell associated with the BONE RESORPTION. An odontoclast, also called cementoclast, is cytomorphologically the same as an osteoclast and is involved in CEMENTUM resorption.
A group of CORTICOSTEROIDS that affect carbohydrate metabolism (GLUCONEOGENESIS, liver glycogen deposition, elevation of BLOOD SUGAR), inhibit ADRENOCORTICOTROPIC HORMONE secretion, and possess pronounced anti-inflammatory activity. They also play a role in fat and protein metabolism, maintenance of arterial blood pressure, alteration of the connective tissue response to injury, reduction in the number of circulating lymphocytes, and functioning of the central nervous system.
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
A plasma protein that circulates in increased amounts during inflammation and after tissue damage.
Deformities of the hand, or a part of the hand, acquired after birth as the result of injury or disease.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Antibodies from non-human species whose protein sequences have been modified to make them nearly identical with human antibodies. If the constant region and part of the variable region are replaced, they are called humanized. If only the constant region is modified they are called chimeric. INN names for humanized antibodies end in -zumab.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging.
Studies in which subsets of a defined population are identified. These groups may or may not be exposed to factors hypothesized to influence the probability of the occurrence of a particular disease or other outcome. Cohorts are defined populations which, as a whole, are followed in an attempt to determine distinguishing subgroup characteristics.
Bone loss due to osteoclastic activity.
An extracellular endopeptidase of vertebrate tissues similar to MATRIX METALLOPROTEINASE 1. It digests PROTEOGLYCAN; FIBRONECTIN; COLLAGEN types III, IV, V, and IX, and activates procollagenase. (Enzyme Nomenclature, 1992)
An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.
Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.
A rare complication of rheumatoid arthritis with autoimmune NEUTROPENIA; and SPLENOMEGALY.
The distal extremity of the leg in vertebrates, consisting of the tarsus (ANKLE); METATARSUS; phalanges; and the soft tissues surrounding these bones.
A method of studying a drug or procedure in which both the subjects and investigators are kept unaware of who is actually getting which specific treatment.
The distal part of the arm beyond the wrist in humans and primates, that includes the palm, fingers, and thumb.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
A double gliding joint formed by the CLAVICLE, superior and lateral parts of the manubrium sterni at the clavicular notch, and the cartilage of the first rib.
The endogenous compounds that mediate inflammation (AUTACOIDS) and related exogenous compounds including the synthetic prostaglandins (PROSTAGLANDINS, SYNTHETIC).
The articulation between a metatarsal bone (METATARSAL BONES) and a phalanx.
An oral chrysotherapeutic agent for the treatment of rheumatoid arthritis. Its exact mechanism of action is unknown, but it is believed to act via immunological mechanisms and alteration of lysosomal enzyme activity. Its efficacy is slightly less than that of injected gold salts, but it is better tolerated, and side effects which occur are potentially less serious.
Chronic inflammatory and autoimmune disease in which the salivary and lacrimal glands undergo progressive destruction by lymphocytes and plasma cells resulting in decreased production of saliva and tears. The primary form, often called sicca syndrome, involves both KERATOCONJUNCTIVITIS SICCA and XEROSTOMIA. The secondary form includes, in addition, the presence of a connective tissue disease, usually rheumatoid arthritis.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX.
A subtype of non-receptor protein tyrosine phosphatases that is characterized by the presence of an N-terminal catalytic domain and a C-terminal PROLINE-rich domain. The phosphatase subtype is predominantly expressed in LYMPHOCYTES and plays a key role in the inhibition of downstream T-LYMPHOCYTE activation. Polymorphisms in the gene that encodes this phosphatase subtype are associated with a variety of AUTOIMMUNE DISEASES.
Hereditary metabolic disorder characterized by recurrent acute arthritis, hyperuricemia and deposition of sodium urate in and around the joints, sometimes with formation of uric acid calculi.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Inflammation of the SPINE. This includes both arthritic and non-arthritic conditions.
Inflammation of the bone.
A species of LENTIVIRUS, subgenus ovine-caprine lentiviruses (LENTIVIRUSES, OVINE-CAPRINE), closely related to VISNA-MAEDI VIRUS and causing acute encephalomyelitis; chronic arthritis; PNEUMONIA; MASTITIS; and GLOMERULONEPHRITIS in goats. It is transmitted mainly in the colostrum and milk.
Treatment of diseases with biological materials or biological response modifiers, such as the use of GENES; CELLS; TISSUES; organs; SERUM; VACCINES; and humoral agents.
Complex pharmaceutical substances, preparations, or matter derived from organisms usually obtained by biological methods or assay.
A class of statistical methods applicable to a large set of probability distributions used to test for correlation, location, independence, etc. In most nonparametric statistical tests, the original scores or observations are replaced by another variable containing less information. An important class of nonparametric tests employs the ordinal properties of the data. Another class of tests uses information about whether an observation is above or below some fixed value such as the median, and a third class is based on the frequency of the occurrence of runs in the data. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1284; Corsini, Concise Encyclopedia of Psychology, 1987, p764-5)
A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B and OSTEOPROTEGERIN. It plays an important role in regulating OSTEOCLAST differentiation and activation.
The level of health of the individual, group, or population as subjectively assessed by the individual or by more objective measures.
Sites on an antigen that interact with specific antibodies.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Methods to determine in patients the nature of a disease or disorder at its early stage of progression. Generally, early diagnosis improves PROGNOSIS and TREATMENT OUTCOME.
Predetermined sets of questions used to collect data - clinical data, social status, occupational group, etc. The term is often applied to a self-completed survey instrument.
An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.
Subset of helper-effector T-lymphocytes which synthesize and secrete IL-17, IL-17F, and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.
The performance of the basic activities of self care, such as dressing, ambulation, or eating.
A thioglucose derivative used as an antirheumatic and experimentally to produce obesity in animals.
Diseases of BONES.
A constitution or condition of the body which makes the tissues react in special ways to certain extrinsic stimuli and thus tends to make the individual more than usually susceptible to certain diseases.
Gram-negative helical bacteria, in the genus BORRELIA, that are the etiologic agents of LYME DISEASE. The group comprises many specific species including Borrelia afzelii, Borellia garinii, and BORRELIA BURGDORFERI proper. These spirochetes are generally transmitted by several species of ixodid ticks.
Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.
Glycoproteins which have a very high polysaccharide content.
An encapsulated lymphatic organ through which venous blood filters.
The surgical fixation of a joint by a procedure designed to accomplish fusion of the joint surfaces by promoting the proliferation of bone cells. (Dorland, 28th ed)
The distance and direction to which a bone joint can be extended. Range of motion is a function of the condition of the joints, muscles, and connective tissues involved. Joint flexibility can be improved through appropriate MUSCLE STRETCHING EXERCISES.
Partial or total replacement of a joint.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Biologically active substances whose activities affect or play a role in the functioning of the immune system.
Combinations of diagnostic or therapeutic substances linked with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; or ANTIGENS. Often the diagnostic or therapeutic substance is a radionuclide. These conjugates are useful tools for specific targeting of DRUGS and RADIOISOTOPES in the CHEMOTHERAPY and RADIOIMMUNOTHERAPY of certain cancers.
Prostheses used to partially or totally replace a human or animal joint. (from UMDNS, 1999)
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.
In horses, cattle, and other quadrupeds, the joint between the femur and the tibia, corresponding to the human knee.
Surgical reconstruction of a joint to relieve pain or restore motion.
Noninflammatory degenerative disease of the knee joint consisting of three large categories: conditions that block normal synchronous movement, conditions that produce abnormal pathways of motion, and conditions that cause stress concentration resulting in changes to articular cartilage. (Crenshaw, Campbell's Operative Orthopaedics, 8th ed, p2019)
Tuberculosis of the bones or joints.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.
Distortion or disfigurement of the foot, or a part of the foot, acquired through disease or injury after birth.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
A hinge joint connecting the FOREARM to the ARM.
A cytokine produced by a variety of cell types, including T-LYMPHOCYTES; MONOCYTES; DENDRITIC CELLS; and EPITHELIAL CELLS that exerts a variety of effects on immunoregulation and INFLAMMATION. Interleukin-10 combines with itself to form a homodimeric molecule that is the biologically active form of the protein.
The total number of cases of a given disease in a specified population at a designated time. It is differentiated from INCIDENCE, which refers to the number of new cases in the population at a given time.
Specific molecular sites on the surface of various cells, including B-lymphocytes and macrophages, that combine with IMMUNOGLOBULIN Gs. Three subclasses exist: Fc gamma RI (the CD64 antigen, a low affinity receptor), Fc gamma RII (the CD32 antigen, a high affinity receptor), and Fc gamma RIII (the CD16 antigen, a low affinity receptor).
Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)
The joint involving the CERVICAL ATLAS and axis bones.
PROTEOGLYCANS-associated proteins that are major components of EXTRACELLULAR MATRIX of various tissues including CARTILAGE; and INTERVERTEBRAL DISC structures. They bind COLLAGEN fibers and contain protein domains that enable oligomer formation and interaction with other extracellular matrix proteins such as CARTILAGE OLIGOMERIC MATRIX PROTEIN.
The joint that is formed by the articulation of the head of FEMUR and the ACETABULUM of the PELVIS.
A generic concept reflecting concern with the modification and enhancement of life attributes, e.g., physical, political, moral and social environment; the overall condition of a human life.
Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.
The age, developmental stage, or period of life at which a disease or the initial symptoms or manifestations of a disease appear in an individual.
A single nucleotide variation in a genetic sequence that occurs at appreciable frequency in the population.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Endoscopic examination, therapy and surgery of the joint.
Presence of calcium salts, especially calcium pyrophosphate, in the cartilaginous structures of one or more joints. When accompanied by attacks of goutlike symptoms, it is called pseudogout. (Dorland, 27th ed)
A family of zinc-dependent metalloendopeptidases that is involved in the degradation of EXTRACELLULAR MATRIX components.
A heterogeneous group of disorders, some hereditary, others acquired, characterized by abnormal structure or function of one or more of the elements of connective tissue, i.e., collagen, elastin, or the mucopolysaccharides.
Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules.
The immovable joint formed by the lateral surfaces of the SACRUM and ILIUM.
The CARPAL BONES; METACARPAL BONES; and FINGER PHALANGES. In each hand there are eight carpal bones, five metacarpal bones, and 14 phalanges.
A class of compounds composed of repeating 5-carbon units of HEMITERPENES.
Large HYALURONAN-containing proteoglycans found in articular cartilage (CARTILAGE, ARTICULAR). They form into aggregates that provide tissues with the capacity to resist high compressive and tensile forces.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
A fluid-filled sac lined with SYNOVIAL MEMBRANE that provides a cushion between bones, tendons and/or muscles around a joint.
Disease having a short and relatively severe course.

Prevention of collagen-induced arthritis by gene delivery of soluble p75 tumour necrosis factor receptor. (1/2364)

Collagen type II-induced arthritis (CIA) in DBA/1 mice can be passively transferred to SCID mice with spleen B- and T-lymphocytes. In the present study, we show that infection ex vivo of splenocytes from arthritic DBA/1 mice with a retroviral vector, containing cDNA for the soluble form of human p75 receptor of tumour necrosis factor (TNF-R) before transfer, prevents the development of arthritis, bone erosion and joint inflammation in the SCID recipients. Assessment of IgG subclass levels and studies of synovial histology suggest that down-regulating the effector functions of T helper-type 1 (Th1) cells may, at least in part, explain the inhibition of arthritis in the SCID recipients. In contrast, the transfer of splenocytes infected with mouse TNF-alpha gene construct resulted in exacerbated arthritis and enhancement of IgG2a antibody levels. Intriguingly, infection of splenocytes from arthritic DBA/1 mice with a construct for mouse IL-10 had no modulating effect on the transfer of arthritis. The data suggest that manipulation of the immune system with cytokines, or cytokine inhibitors using gene transfer protocols can be an effective approach to ameliorate arthritis.  (+info)

Modulation of acute and chronic inflammatory processes by cacospongionolide B, a novel inhibitor of human synovial phospholipase A2. (2/2364)

1. Cacospongionolide B is a novel marine metabolite isolated from the sponge Fasciospongia cavernosa. In in vitro studies, this compound inhibited phospholipase A2 (PLA2), showing selectivity for secretory PLA2 (sPLA2) versus cytosolic PLA2 (cPLA2), and its potency on the human synovial enzyme (group II) was similar to that of manoalide. 2. This activity was confirmed in vivo in the 8 h zymosan-injected rat air pouch, on the secretory enzyme accumulating in the pouch exudate. Cacospongionolide B, that is bioavailable when is given orally, reduced the elevated levels of sPLA2 present in paw homogenates of rats with adjuvant arthritis. 3. This marine metabolite showed topical anti-inflammatory activity on the mouse ear oedema induced by 12-O-tetradecanoylphorbol acetate (TPA) and decreased carrageenin paw oedema in mice after oral administration of 5, 10 or 20 mg kg(-1). 4. In the mouse air pouch injected with zymosan, cacospongionolide B administered into the pouch, induced a dose-dependent reduction in the levels of eicosanoids and tumour necrosis factor alpha (TNFalpha) in the exudates 4 h after the stimulus. It also had a weak effect on cell migration. 5. The inflammatory response of adjuvant arthritis was reduced by cacospongionolide B, which did not significantly affect eicosanoid levels in serum, paw or stomach homogenates and did not induce toxic effects. 6 Cacospongionolide B is a new inhibitor of sPLA2 in vitro and in vivo, with anti-inflammatory properties in acute and chronic inflammation. This marine metabolite was active after oral administration and able to modify TNFalpha levels, and may offer an interesting approach in the search for new anti-inflammatory agents.  (+info)

Localization of non-Mhc collagen-induced arthritis susceptibility loci in DBA/1j mice. (3/2364)

One approach to understanding common human diseases is to determine the genetic defects responsible for similar diseases in animal models and place those defective genes in their corresponding biochemical pathways. Our laboratory is working with an animal model for human rheumatoid arthritis called collagen-induced arthritis (CIA). We are particularly interested in determining the location of disease-predisposing loci. To that end, we performed experiments to localize susceptibility loci for CIA in an F2 cross between the highly susceptible mouse strain DBA/1j and the highly resistant mouse strain SWR/j. Specifically, a quantitative trait locus analysis was performed to localize regions of the mouse genome responsible for susceptibility/severity to CIA. One susceptibility locus, Cia1 in the major histocompatibility locus, had been identified previously. Two additional loci were detected in our analysis that contribute to CIA severity (Cia2, Cia3) on chromosomes 2 and 6. A third locus was detected that contributes to the age of onset of the disease. This locus (Cia4) was located on chromosome 2 and was linked to the same region as Cia2. Determining the identity of these loci may provide insights into the etiology of human rheumatoid arthritis.  (+info)

Effects of petrosaspongiolide M, a novel phospholipase A2 inhibitor, on acute and chronic inflammation. (4/2364)

The marine product petrosaspongiolide M is a novel inhibitor of phospholipase A2 (PLA2), showing selectivity for secretory PLA2 versus cytosolic PLA2, with a potency on the human synovial enzyme (group II) similar to that of manoalide. This compound was more potent than manoalide on bee venom PLA2 (group III) and had no effect on group I enzymes (Naja naja and porcine pancreatic PLA2). Inhibition of PLA2 was also observed in vivo in the zymosan-injected rat air pouch, on the secretory enzyme accumulated in the pouch exudate. Petrosaspongiolide M decreased carrageenan paw edema in mice after the oral administration of 5, 10, or 20 mg/kg. This marine metabolite (0.01-1.0 micromol/pouch) induced a dose-dependent reduction in the levels of prostaglandin (PG)E2, leukotriene B4, and tumor necrosis factor-alpha in the mouse air pouch injected with zymosan 4 h after the stimulus. It also had a weaker effect on cell migration. The inflammatory response of adjuvant arthritis was reduced by petrosaspongiolide M, which also inhibited leukotriene B4 levels in serum and PGE2 levels in paw homogenates. In contrast with indomethacin, this marine compound did not reduce PGE2 levels in stomach homogenates. Petrosaspongiolide M is a new inhibitor of secretory PLA2 in vitro and in vivo, with anti-inflammatory properties in acute and chronic inflammation.  (+info)

Efficacy of sustained blood levels of interleukin-1 receptor antagonist in animal models of arthritis: comparison of efficacy in animal models with human clinical data. (5/2364)

OBJECTIVE: To determine the role of interleukin-1 receptor antagonist (IL-1Ra) in rat adjuvant arthritis and rat type II collagen-induced arthritis, and to compare the efficacy in rat models with that seen in human clinical trials of IL-1Ra. METHODS: Rats with developing adjuvant arthritis or established collagen-induced arthritis were treated with IL-1Ra by continuous infusion in order to determine and maintain efficacious blood levels of this IL-1 inhibitory protein in the rats for comparison with human clinical data. The effects of treatment in the rats were monitored by sequential caliper measurement of the ankle joints, determination of final paw weights, and histologic evaluation with particular emphasis on bone and cartilage lesions. The effects of IL-1Ra on joint swelling and radiographic bone damage in patients with rheumatoid arthritis (RA) in a 6-month trial were compared with the findings in rats. RESULTS: Dramatic differences in the profile of IL-1Ra activity were seen between the 2 groups of rats. Modest antiinflammatory effects were observed in the adjuvant arthritis rats treated with IL-1Ra. However, marked inhibition of bone resorption occurred, even at doses with which antiinflammatory activity was not seen. In contrast, IL-1Ra treatment of rats with established collagen-induced arthritis resulted in nearly complete suppression of all aspects of the disease when adequate blood levels of IL-1Ra were maintained. Treatment of RA patients with IL-1Ra (150 mg daily) resulted in modest inhibition of joint swelling and inhibition of radiographic progression of bone lesions. CONCLUSION: IL-1 appears to be of major importance in mediating the bone resorption that occurs in rat adjuvant arthritis, but is less important in the pathogenesis of periarticular inflammation in this disease. In contrast, IL-1 is of major importance in mediating all aspects of disease progression in rat collagen-induced arthritis. Similar to the response in adjuvant arthritic rats, RA patients treated with IL-1Ra showed only modest antiinflammatory activity, but had evidence of inhibition of progression of bone resorption. However, a comparison of the plasma levels of IL-1Ra in humans and rats suggests that the optimal level of dosing for continuous saturation of IL-1 receptors may not have been achieved in humans, although this was achieved in the rat studies.  (+info)

Somatostatin receptor subtype expression in cells of the rat immune system during adjuvant arthritis. (6/2364)

Somatostatin is a neuropeptide that is widely distributed throughout the body. It acts as a neurohormone and a neurotransmitter and may also have an immunomodulatory role. The genes for five subtypes of somatostatin receptors (sst) have been cloned, suggesting that the diverse effects of the peptide might be mediated by different receptors. We are interested in studying the role of sst ininflammation, using an animal model. Because of the up-regulation of sst expression in inflamed joints in human rheumatoid arthritis, we chose rat adjuvant arthritis as an experimental model. In order to determine which of the sst subtypes might be important in immune modulation, subtype expression in leukocytes isolated from different lymphoid tissues of the rat was studied. Also, the expression levels of the most abundantly expressed sst mRNAs in leukocytes from spleen and blood were compared in rats with adjuvantarthritis and controls, using a semi-quantitative approach. Furthermore, the effect of systemic administration of a long-acting somatostatin analogue, octreotide, which binds selectively to sst subtypes 2 and 5 (sst2 and sst5), on the incidence and the severity of rat adjuvant arthritis, was studied. The main sst expressed in cells of the rat immune system, both resting and activated, were found to be sst3 and sst4. This contrasts with the human and murine situations, in which sst2 appears to be the main subtype expressed in the immune system. No quantitative differences in sst subtype mRNA levels in leukocytes from spleen and blood were found between rats with adjuvant arthritis and controls. Finally, no effect of systemic administration of octreotide on either the incidence or severity of adjuvant arthritis in Lewis rats was found. As octreotide binds selectively to sst2 and sst5, the absence of an immunomodulatory effect of this analogue in rat adjuvant arthritis corroborates our finding that these sst subtypes are not expressed in cells of the rat immune system. In conclusion, cells of the rat immune system appear to express a spectrum of sst (sst3 and sst4) different from that found in human granulomatous and autoimmune disease (mainly sst2). Therefore, the rat adjuvant arthritis model appears to be suitable only for studying the immunomodulatory effects of somatostatin analogues which have a high affinity for sst3 and sst4, but not for studying the immunomodulatory effects of octreotide, which has a high affinity only for sst2 and sst5.  (+info)

Prevention of collagen-induced arthritis in mice by a polyphenolic fraction from green tea. (7/2364)

Identification of common dietary substances capable of affording protection or modulating the onset and severity of arthritis may have important human health implications. An antioxidant-rich polyphenolic fraction isolated from green tea (green tea polyphenols, GTPs) has been shown to possess anti-inflammatory and anticarcinogenic properties in experimental animals. In this study we determined the effect of oral consumption of GTP on collagen-induced arthritis in mice. In three independent experiments mice given GTP in water exhibited significantly reduced incidence of arthritis (33% to 50%) as compared with mice not given GTP in water (84% to 100%). The arthritis index also was significantly lower in GTP-fed animals. Western blot analysis showed a marked reduction in the expression of inflammatory mediators such as cyclooxygenase 2, IFN-gamma, and tumor necrosis factor alpha in arthritic joints of GTP-fed mice. Histologic and immunohistochemical analysis of the arthritic joints in GTP-fed mice demonstrated only marginal joint infiltration by IFN-gamma and tumor necrosis factor alpha-producing cells as opposed to massive cellular infiltration and fully developed pannus in arthritic joints of non-GTP-fed mice. The neutral endopeptidase activity was approximately 7-fold higher in arthritic joints of non-GTP-fed mice in comparison to nonarthritic joints of unimmunized mice whereas it was only 2-fold higher in the arthritic joints of GTP-fed mice. Additionally, total IgG and type II collagen-specific IgG levels were lower in serum and arthritic joints of GTP-fed mice. Taken together our studies suggest that a polyphenolic fraction from green tea that is rich in antioxidants may be useful in the prevention of onset and severity of arthritis.  (+info)

Specific targeting of activated endothelium in rat adjuvant arthritis with a 99mTc-radiolabeled E-selectin-binding peptide. (8/2364)

OBJECTIVE: To determine the potential of an E-selectin-binding peptide (ESbp) to specifically bind activated endothelium in rheumatoid arthritis (RA) animal models. METHODS: ESbp (KYDGDITWDQLWDLMK; 2,027 daltons) was labeled with biotin and 99mTc. The affinity of ESbp derivatives for E-selectin was measured by enzyme-linked immunosorbent assay. The binding of biotin-ESbp was compared with that of an anti-E-selectin antibody, by immunohistochemical analyses of human synovial sections and sections from the Mycoplasma pulmonis MRL-lpr/lpr mouse arthritis model. 99mTc-ESbp was sequentially imaged in vivo with a gamma camera in the rat adjuvant-induced arthritis model. RESULTS: E-selectin expression was detected in human RA synovium and mouse arthritic synovium using biotin-ESbp. Both biotin-ESbp and 99mTc-labeled ESbp had high affinity for E-selectin (dissociation constant 2-5 nM). In vivo imaging showed specific binding of 99mTc-ESbp to the rat ankle joint prior to clinical manifestations of inflammation. CONCLUSION: These results demonstrate that activated endothelium can be targeted with 99mTc-ESbp. The specificity of targeting can be used to evaluate up-regulation of E-selectin in RA models, and to follow changes in this up-regulation during treatment trials.  (+info)

The purpose of this study was to investigate the lesions of a mouse collagen antibody-induced arthritis (CAIA) model using fluorescence bioimaging and micro-computed tomography (micro-CT) and to compare it with histopathological examination. Twelve mice were randomly divided into three groups: group 1 (G1) as control, group 2 (G2) as fluorescence probe control and group 3 (G3) as collagen antibody-induced arthritis. The mice of G3 intravenously received anti-type II collagen 5-clone antibody cocktail (2 mg/mouse) on day 0 and intraperitoneally received lipopolysaccharide (|TEX|$50{\mu}g/mouse$|/TEX|) on day 3. On the while, the mice of G1 and G2 received 0.9% saline in equal volumes at equivalent times. Fluorescence bioimaging and micro-CT analysis were carried out to assess arthritis. Treatment with the collagen antibody cocktail increased the paw thickness of mice compared to those in both the control and probe-treated groups. Fluorescence bioimaging using a near infrared imaging agent showed high
Les Produits de Chondrex Modèles animaux Anticorps Tests danticorps Recherche sur les bactéries Recherche contre le cancer Collagène Collagénase HMGB1 Dosages de protéines Coloration des tissus Tampons dimmunoanalyse Métabolisme cellulaire Les MSDS ou fiches de données de sécurités Protocoles Gentaur Assay kits en collaboration Animal Model Protocols Arthritis Scoring System Collagen Antibody-Induced Arthritis - Mouse (cat# 53010, 53040, 53100) Collagen-Induced Arthritis - Mouse Collagen-Induced Arthritis - Mouse (Visual Guide) Collagen-Induced Arthritis - Rat Adjuvant-Induced Arthritis - […]. ...
Studies in IL-6-deficient (IL-6-/-) mice highlight that IL-6 contributes to arthritis progression. However, the molecular mechanism controlling its activity in vivo remains unclear. Using an experimental arthritis model in IL-6-/- mice, we have established a critical role for the soluble IL-6R in joint inflammation. Although intra-articular administration of IL-6 itself was insufficient to reconstitute arthritis within these mice, a soluble IL-6R-IL-6 fusion protein (HYPER-IL-6) restored disease activity. Histopathological assessment of joint sections demonstrated that HYPER-IL-6 increased arthritis severity and controlled intrasynovial mononuclear leukocyte recruitment through the CC-chemokine CCL2. Activation of synovial fibroblasts by soluble IL-6R and IL-6 emphasized that these cells may represent the source of CCL2 in vivo. Specific blockade of soluble IL-6R signaling in wild-type mice using soluble gp130 ameliorated disease. Consequently, soluble IL-6R-mediated signaling represents a ...
The Rho family GTPase Rac1 regulates cytoskeletal rearrangements crucial for the recruitment, extravasation and activation of leukocytes at sites of inflammation. Rac1 signaling also promotes the activation and survival of lymphocytes and osteoclasts. Therefore, we assessed the ability of a cell-permeable Rac1 carboxy-terminal inhibitory peptide to modulate disease in mice with collagen-induced arthritis (CIA). CIA was induced in DBA/1 mice, and in either early or chronic disease, mice were treated three times per week by intraperitoneal injection with control peptide or Rac1 inhibitory peptide. Effects on disease progression were assessed by measurement of paw swelling. Inflammation and joint destruction were examined by histology and radiology. Serum levels of anti-collagen type II antibodies were measured by enzyme-linked immunosorbent assay. T-cell phenotypes and activation were assessed by fluorescence-activated cell sorting analysis. Results were analyzed using Mann-Whitney U and unpaired Student
Methods Expression levels of Mincle mRNA was determined in inflamed paws from hTNFtg and IL1-/-IL6-/-hTNFtg mice by qPCR. To study the role of mincle deficiency in experimental arthritis, we generated mincle-/-hTNFtg mice as well as their hTNFtg littermates. Mice were weekly assessed for clinical signs of arthritis from week 4 to 11 after birth. We quantitatively analysed inflammatory joint destruction in H&E, TRAP and TB stained sections from hind paws. The effect of mincledeficiency was also evaluated in the K/BxN serum transfer model. Therefore, mincle-/- and mincle+/+ mice received i.v. injections of K/BxN serum on day 1 and day 3. Animals were assessed from day1 to 12 for clinical signs of arthritis. Quantitative histopathological analysis were performed as above mentioned in hind paws.. ...
TY - JOUR. T1 - Inhibition of Notch signalling ameliorates experimental inflammatory arthritis. AU - Park, Jong Sung. AU - Kim, Seol Hee. AU - Kim, Kwang Meyung. AU - Jin, Cheng Hao. AU - Choi, Ki Young. AU - Jang, Jiyeon. AU - Choi, Yuri. AU - Gwon, A. Ryeong. AU - Baik, Sang Ha. AU - Yun, Ui Jeong. AU - Chae, Su Young. AU - Lee, Seulki. AU - Kang, Young Mo. AU - Lee, Kang Choon. AU - Arumugam, Thiruma V.. AU - Mattson, Mark P.. AU - Park, Jae Hyung. AU - Jo, Dong Gyu. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Objective: To test the hypothesis that Notch signalling plays a role in the pathogenesis of rheumatoid arthritis (RA) and to determine whether pharmacological inhibition of Notch signalling with γ-secretase inhibitors can ameliorate the RA disease process in an animal model.Methods Collagen-induced arthritis was induced in C57BL/6 or Notch antisense transgenic mice by immunisation with chicken type II collagen (CII). C57BL/6 mice were administered with different doses of inhibitors of ...
The use of regulatory or immature dendritic cells (DCs) as tools for modulating experimental rheumatoid arthritis is very recent. TNF-stimulated DCs have been shown to restore tolerance in experimental autoimmune encephalomyelitis and collagen-induced arthritis (CIA).. Objective: We investigated the capacity of short-term lipopolysaccharide (LPS)-stimulated DCs pulsed with type-II collagen (CII) to induce tolerance against established CIA.. Methods: Bone marrow-derived DCs were generated in the presence of GM-CSF. After CIA induction, mice were injected at day 35 with a single dose of 4- or 24-hour LPS-stimulated DCs that had been loaded with CII (CII/DCs, 4hLPS/CII/DCs or 24hLPS/CII/DCs). Arthritis progression was monitored by clinical and histologic evaluations.. Results Flow cytometry of 4hLPS/CII/DCs showed intermediate CD40 and CD86 expression, lower than that of 24hLPS/CII/DCs (fully mature) and higher than that of CII/DCs (immature). A functional assay showed that 4hLPS/CII/DCs display ...
Experimental Arthritis: ARTHRITIS that is induced in experimental animals. Immunological methods and infectious agents can be used to develop experimental arthritis models. These methods include injections of stimulators of the immune response, such as an adjuvant (ADJUVANTS, IMMUNOLOGIC) or COLLAGEN.
Most antirheumatic therapies are given systemically, which may result in complications because of the high dosage needed to achieve therapeutic levels in the joints. Gene therapy might provide a more efficient system to deliver therapeutic compounds at the site of inflammation. The artificial chromosome expression system (ACes) is a unique, nonintegrating, nonviral gene-expression system, which functions like a natural chromosome. This technology offers advantages over current expression systems because it allows stable expression of genes producing single or multiple proteins over long periods. We are developing ex vivo gene therapy using artificial chromosomes containing reporter genes (LacZ or RFP) for local delivery of genes in rats with adjuvant arthritis. The aim of this study was to evaluate the transfection efficiency in cell lines or primary cells, including rat skin fibroblasts (RSFs) and fibroblast-like synoviocytes (FLSs). Furthermore, we investigated the feasibility of local ...
There is considerable evidence implicating tumor necrosis factor alpha (TNF-alpha) in the pathogenesis of rheumatoid arthritis. This evidence is based not only on the universal presence of TNF-alpha in arthritic joints accompanied by the upregulation of TNF-alpha receptors but also on the effects of neutralizing TNF-alpha in joint cell cultures. Thus, neutralization of TNF-alpha in vitro results in inhibition of the production of interleukin 1, which like TNF-alpha, is believed to contribute to joint inflammation and erosion. To determine the validity of this concept in vivo, the effect of administering TNF-neutralizing antibodies to mice with collagen-induced arthritis has been studied. This disease model was chosen because of its many immunological and pathological similarities to human rheumatoid arthritis. TN3-19.12, a hamster IgG1 monoclonal antibody to murine TNF-alpha/beta, was injected i.p. into mice either before the onset of arthritis or after the establishment of clinical disease. Anti-TNF
It has been demonstrated that pterostilbene inhibits reactive oxygen species production in neutrophils in vitro. However, little is known about its effects on neutrophils during inflammation in vivo. In this study, the effect of pterostilbene on neutrophil activity was investigated in experimental arthritis model. Lewis rats were injected by a single intradermal injection of heat-killed Mycobacterium butyricum in Freunds adjuvant to develop arthritis. Another group of arthritic animals received pterostilbene 30mg/kg, daily, p.o. The number and activity of neutrophils in blood were measured on a weekly basis during the whole experiment. Moreover, the total radical trapping potential in plasma was measured at the end of the experiment. In the pterostilbene treated arthritic group, the treatment significantly lowered the number of neutrophils in blood on days 14 and 21 without significant downregulation of neutrophil oxidative burst. Pterostilbene nonsignificantly increased total radical trapping ...
A rapid and synchronized alternative to the CIA and K/BxN models that reduces variability, eliminates the need for expensive colonies and conserves test compound, controls and vivarium space.
Incidence and severity of K/BxN serum transfer-induced arthritis are not reduced in IL-36 receptor (R) knockout (KO) mice. Incidence of arthritis (A), arthritis
Fingerprint Dive into the research topics of Transforming growth factor β-transduced mesenchymal stem cells ameliorate experimental autoimmune arthritis through reciprocal regulation of Treg/Th17 cells and osteoclastogenesis. Together they form a unique fingerprint. ...
DIAS, Renata Gonçalves; SAMPAIO, Sandra Coccuzzo; SANTANNA, Morena Brazil; et al. Articular inflammation induced by an enzymatically-inactive Lys49 phospholipase A2: activation of endogenous phospholipases contributes to the pronociceptive effect. Journal of Venomous Animals and Toxins including Tropical Diseases, London, v. 23, p. 13 , 2017. Disponível em: < http://dx.doi.org/10.1186/s40409-017-0104-0 > DOI: 10.1186/s40409-017-0104-0 ...
In humans and in animal models, susceptibility to arthritis is under complex genetic control, reflecting influences on the immunological processes that initiate autoimmunity and on subsequent inflammatory mechanisms in the joints. The effector phases are conveniently modeled by the K/BxN serum transfer system, a robust model well suited for genetic analysis where arthritis is initiated by pathogenic Ig. Here, we mapped the genetic loci distinguishing the high-responder BALB/c vs. low-responder SJL strains. After computational modeling of potential breeding schemes, we adapted a stepwise selective breeding strategy, with a whole-genome scan performed on a limited number of animals. Several genomic regions proved significantly associated with high sensitivity to arthritis. One of these regions, on distal chr2, was centered on the interleukin 1 gene family. Quantitation of transcripts of the Il1a and Il1b candidate genes revealed a 10-fold greater induction of Il1b mRNA in BALB/c than in SJL
The DNA binding activity of AP-1 and NF-kappaB are markedly increased in both CIA and RA. In CIA, activation of AP-1 and NF-kappaB precede both clinical arthritis and metalloproteinase gene expression. NF-kappaB expression correlated better than AP-1 with metalloproteinase expression.
Second, in order to investigate whether other MHC genes also contribute to arthritis susceptibility, we assessed arthritis development in congenic strains mapped to other parts of the MHC region. We identified a second arthritis-regulatory QTL in the MHC class III region, that regulates not only the onset and severity, but also chronicity of arthritis. We subsequently mapped this effect to a conserved, 33-kb large haplotype Ltab-Ncr3 comprising five polymorphic genes. Interestingly, unlike other positionally-identified arthritis genes in rats, Ltab-Ncr3 regulates only adjuvant arthritis models but not autoimmunity triggered by specific tissue antigens, such as type II collagen. Furthermore, we found that gene expression and alternative splicing of the Ltab-Ncr3 genes correlate remarkably with arthritis severity and some of the gene expression differences were reproduced in a cohort of RA patients and healthy controls ...
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Peptide analogues of disease-associated epitopes were studied for inhibition of experimental allergic encephalomyelitis (EAE) and adjuvant arthritis (AA) in Lewis rats. EAE- and AA-associated analogues were selected as competitors because of their in vitro inhibitory activity on proliferation of encephalitogenic and arthritogenic T cells. Although the EAE-associated competitor had a superior major histocompatibility complex (MHC) binding affinity, the AA-associated competitor was a better inhibitor of the in vitro proliferation of arthritogenic T cells. Furthermore, although in vivo EAE was inhibited by both competitors, AA was only inhibited by the AA-associated competitor. Remarkably, in contrast to what was expected of a regular MHC competitor peptide, the AA-associated peptide analogue also prevented AA upon immunization before disease induction and appeared to induce T cell responses that crossreacted with the original disease-associated epitope. Therefore, it is concluded that ...
In collagen-induced arthritis (CIA) of mouse, self-reactive T cells recognize a peptide antigen from type II collagen (CII). CII is of particular interest, as an autoimmune response to this protein leads to CIA in mice, rats, and primates. Activation of T cells is believed to be an important pathogenic factor in autoimmune disease. So, T cells have become a focal point of study for the development of novel therapeutic approaches to the treatment of autoimmune disease. In this study, we evaluated the efficacy and mechanism of recombinant MHC II molecules in regulation of the antigen-specific T cell clones by using mouse I-Aq, combined with an auto-antigen peptide from type II collagen (CII260-274) in CIA model. It was found that recombinant I-Aq/CII260-274 molecules not only activate CII-specific T cell clone but also inhibit the same clone in vitro according to the condition of stimulation. Furthermore, development of CIA in mice was successfully prevented by the in vivo injection of a ...
Celastrol exhibits anti-arthritic effect in rheumatoid arthritis (RA), but the role of celastrol-mediated Ca mobilization in treatment of RA remains unelucidated. Here, we illustrate the regulatory role of celastrol-induced Ca signalling in synovial fibroblasts of RA patients and adjuvant-induced arthritis (AIA) in rats. Molecular target of celastrol was determined by computational docking, Ca dynamic and functional assays on SERCA. Ca -mediated autophagy in RASFs/RAFLS and the underlying mechanism were verified by quantification of endogenous LC3-II puncta, immunoblotting, and flow cytometry with the Ca chelator (BAPTA/AM) or suitable inhibitors. The anti-arthritic effect of celastrol, autophagy induction and growth rate of synovial fibroblasts in AIA rats were monitored by microCT and immunofluorescence staining. mRNA from joint tissues of AIA rats was isolated for transcriptional analysis of inflammatory genes. The role of Ca in regulating the identified genes was investigated by knockdown of ...
Celastrol exhibits anti-arthritic effect in rheumatoid arthritis (RA), but the role of celastrol-mediated Ca mobilization in treatment of RA remains unelucidated. Here, we illustrate the regulatory role of celastrol-induced Ca signalling in synovial fibroblasts of RA patients and adjuvant-induced arthritis (AIA) in rats. Molecular target of celastrol was determined by computational docking, Ca dynamic and functional assays on SERCA. Ca -mediated autophagy in RASFs/RAFLS and the underlying mechanism were verified by quantification of endogenous LC3-II puncta, immunoblotting, and flow cytometry with the Ca chelator (BAPTA/AM) or suitable inhibitors. The anti-arthritic effect of celastrol, autophagy induction and growth rate of synovial fibroblasts in AIA rats were monitored by microCT and immunofluorescence staining. mRNA from joint tissues of AIA rats was isolated for transcriptional analysis of inflammatory genes. The role of Ca in regulating the identified genes was investigated by knockdown of ...
Abs to the α-chain of the IL-2 receptor (anti-CD25) are used clinically to achieve immunosuppression. Here we investigated the effects of DNA vaccination with the whole CD25 gene on the induction of rat adjuvant arthritis. The DNA vaccine protected the rats and led to a shift in the cytokine profile of T cells responding to disease target antigens from Th1 to Th2. The mechanism of protection was found to involve the induction of an antiergotypic response, rather than the induction of anti-CD25 Abs. Antiergotypic T cells respond to activation molecules, ergotopes, expressed on syngeneic activated, but not resting, T cells. CD25-derived peptides function as ergotopes that can be recognized by the antiergotypic T cells. Antiergotypic T cells taken from control sick rats did not proliferate against activated T cells and secreted mainly IFN-γ. In contrast, antiergotypic cells from CD25-DNA-protected rats proliferated against activated T cells and secreted mainly IL-10. Protective antiergotypic T ...
Animals, Antibodies; Monoclonal/pharmacology, Antigen-Antibody Complex/immunology, Arthritis; Experimental/genetics/*immunology, Collagen Type II/immunology, Comparative Study, Genetic Predisposition to Disease, Immunoglobulin G/*metabolism, Macrophages; Peritoneal/*immunology, Mice, Mice; Mutant Strains, Receptors; IgG/antagonists & inhibitors/genetics/*metabolism, Research Support; Non-U.S. Govt ...
Rhomboid family member 2 gene (Rhbdf2) is an inactive homologue lacking essential catalytic residues of rhomboid intramembrane serine proteases. The protein is necessary for maturation of tumor necrosis factor-alpha (TNF-α) converting enzyme, which is the molecule responsible for the release of TNF-α. In this study, Rhbdf2 knockout (KO) mice were produced by CRISPR/CAS9. To see the effects of the failure of TNF-α release induced by Rhbdf2 gene KO, collagen-induced arthritis (CIA), which is the representative TNF-α related disease, was induced in the Rhbdf2 mutant mouse using chicken collagen type II. The severity of the CIA was measured by traditional clinical scores and histopathological analysis of hind limb joints. A rota-rod test and grip strength test were employed to evaluate the severity of CIA based on losses of physical functions. The results indicated that Rhbdf2 mutant mice showed clear alleviation of the clinical severity of CIA as demonstrated by the significantly lower severity ...
A risk score encompassing clinical characteristics, serologic findings, and imaging tests could be used to predict which antibody-positive patients are likely to go on to develop rheumatoid arthritis,
Destruction of cartilage and bone is a poorly managed hallmark of human rheumatoid arthritis (RA). p38 Mitogen-activated protein kinase (MAPK) has been shown to regulate key proinflammatory pathways in RA, including tumor necrosis factor α, interleukin (IL)-1β, and cyclooxygenase-2, as well as the process of osteoclast differentiation. Therefore, we evaluated whether a p38α MAPK inhibitor, indole-5-carboxamide (SD-282), could modulate cartilage and bone destruction in a mouse model of RA induced with bovine type II collagen [collagen-induced arthritis (CIA)]. In mice with early disease, SD-282 treatment significantly improved clinical severity scores, reduced bone and cartilage loss, and reduced mRNA levels of proinflammatory genes in paw tissue, including IL-1β, IL-6, and cyclooxygenase-2. Notably, SD-282 treatment of mice with advanced disease resulted in significant improvement in clinical severity scoring and paw swelling, a reversal in bone and cartilage destruction as assessed by ...
Mediators of Inflammation is a peer-reviewed, Open Access journal that publishes original research and review articles on all types of inflammatory mediators, including cytokines, histamine, bradykinin, prostaglandins, leukotrienes, PAF, biological response modifiers and the family of cell adhesion-promoting molecules.
These findings raise the important question of why TNFα blockade is effective in reducing disease activity despite increasing numbers of Th1 and Th17 cells. To address this question, we used an adoptive transfer system established previously (15) to confirm that the expanded Th1/Th17 cells were potentially pathogenic. Spleen and LN cells from arthritic mice treated for 10-14 d with anti-TNFα mAb or control Ab were pooled and injected into CB-17 SCID mice (5 × 107 cells/mouse or 107 cells/mouse). The SCID recipients were also injected i.p. with 100 μg of type II collagen without adjuvant, which is required for the successful transfer of arthritis (15). The proportion of LN cells to spleen cells was ∼1:10 and was identical in both anti-TNFα-treated and control groups.. Transfer of 5 × 107 cells from either anti-TNFα-treated or control mice led to efficient transfer of arthritis, although onset of arthritis was much earlier when donor cells were derived from anti-TNFα-treated mice (Fig. ...
Find anti inflammatory antiarthritic effects. Mississsippi Stem Cell Therapy Center promotes stem cell therapy and regenerative medicine.
Immune and inflammatory systems are controlled by multiple cytokines, including ILs and INFs. These cytokines exert their biological functions through Janus tyrosine kinases and STAT transcription factors. One such cytokine, IL-6, has been proposed to contribute to the development of rheumatoid arthritis (RA). We found that STAT3 was strongly tyrosine phosphorylated in synovial tissue of RA patients, but not those with osteoarthritis. Blockade of the IL-6-gp130-JAK-STAT3-signaling pathway might therefore be beneficial in the treatment of RA. We show here that the mRNA for the endogenous cytokine signaling repressor CIS3/SOCS3 is abundantly expressed in RA patients. To determine whether CIS3 is effective in treating experimental arthritis, a recombinant adenovirus carrying the CIS3 cDNA was injected periarticularly into the ankle joints of mice with antigen-induced arthritis or collagen-induced arthritis (CIA). Periarticular injection of CIS3 adenovirus drastically reduced the severity of ...
The 60 kDa heat shock protein (HSP60) has been reported to influence T-cell responses in two ways: as a ligand of toll-like receptor 2 signalling and as an antigen. Here we describe a new mechanism of T-cell immuno-regulation focused on HSP60: HSP60 is up-regulated and presented by activated T cells (HSP60 is an ergotope) to regulatory (anti-ergotypic) T cells. Presentation of HSP60 by activated T cells was found to be MHC-restricted and dependent on accessory molecules - CD28, CD80 and CD86. Anti-ergotypic T cells responded to T-cell HSP60 by proliferation and secreted IFNγ and TGFβ1. In vitro, the anti-ergotypic T cells inhibited IFNγ production by their activated T-cell targets. In vivo, adoptive transfer of an anti-ergotypic HSP60-specific T-cell line led to decreased secretion of IFNγ by arthritogenic T cells and ameliorated adjuvant arthritis (AA). Thus, the presentation of HSP60 by activated T cells turns them into targets for anti-ergotypic regulatory T cells specific for HSP60. ...
(a)-(c) Typical histological appearance of CIA mouse paws: (a) severe inflammatory cellular infiltration-synovitis and pannus formation; (b) bone and cartil
Aggravation of Cold-Restraint Stress-Induced Gastric Lesions in Adjuvant Arthritic Rats : Pathogenic Role of Inducible and Endothelial Nitric Oxide (2009 ...
BioAssay record AID 184149 submitted by ChEMBL: Inhibition of streptococcal cell wall-washed arthritis model in rats at 30.0 mg/kg peroral dose.
Rheumatoid arthritis (RA) is characterized by progressive inflammation of the synovial joints that leads to the breakdown of cartilage and bone …
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TY - JOUR. T1 - Effective treatment of established murine collagen-induced arthritis by systemic administration of dendritic cells genetically modified to express IL-4. AU - Kim, S. H.. AU - Kim, S.. AU - Evans, C. H.. AU - Ghivizzani, S. C.. AU - Oligino, T.. AU - Robbins, P. D.. N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 2001/3/1. Y1 - 2001/3/1. N2 - Dendritic cells (DC) are APCs that are able to stimulate or inhibit immune responses, depending on levels of expression of MHC class I and II costimulatory molecules and cytokines. Our previous studies have suggested that the observed contralateral effect, where injection of a vector carrying certain immunomodulatory genes into one joint resulted in inhibition of arthritis in untreated joints, is mediated by in vivo modification of DC. Therefore, we have examined the ability of genetically modified DC to suppress established murine collagen-induced arthritis (CIA) after i.v. delivery. IL-4 has been shown to partially ...
TY - JOUR. T1 - Successful gene therapy via intraarticular injection of adenovirus vector containing CTLA4IgG in a murine model of type II collagen-induced arthritis. AU - Ijima, K.. AU - Murakami, M.. AU - Okamoto, H.. AU - Inobe, M.. AU - Chikuma, S.. AU - Saito, I.. AU - Kanegae, Y.. AU - Kawaguchi, Y.. AU - Kitabatake, A.. AU - Uede, T.. PY - 2001/8/9. Y1 - 2001/8/9. N2 - We previously constructed an adenovirus vector carrying a gene encoding a soluble form of fusion protein, consisting of the extracellular portion of cytotoxic lymphocyte antigen 4 (CTLA4) and the Fc portion of human immunoglobulin G1 (Adex1CACTLA4IgG). Murine type II collagen-induced arthritis (CIA) was treated with Adex1CACTLA4IgG. A single intraarticular injection of 1 × 105 PFU was able to support serum CTLA4IgG at more than 10 μg/ml for at least 12 weeks and was able to inhibit the CIA clinically and histo-logically. In contrast, intravenous, intramuscular, or subcutaneous injection of 1 × 105 PFU was unable to ...
Abstract: To evaluate the effect of methotrexate on collagen-induced arthritis, micro-computed tomography (micro-CT) and histopathological analyses were used in male Wistar rats. Rats were divided randomly into three groups. Group 1 was treated with 0.9% saline, and groups 2 and 3 were boosted with type Ⅱ collagen. From day 21 to 42, groups 1 and 2 were orally treated with 0.9% saline and group 3 was orally treated with 1.5 mg/kg methotrexate. All rats were sacrificed at day 42 after the first collagen treatment. Micro-CT analyses showed bony parameters, such as bone volume and trabecular number, were decreased in group 2 compared to group 1, and these parameters were recovered in group 3. Histopathological examination and pathological parameter scoring showed that the knee joints of rats in group 2 had severe joint destruction, showing cartilage and bone erosion, enlarged cavities with inflammatory cell infiltration and activation of synovial fibroblasts. By contrast, these changes were ...
TY - JOUR. T1 - Anti-inflammatory effects of licorice and roasted licorice extracts on TPA-induced acute inflammation and collagen-induced arthritis in mice. AU - Chung, Won Yoon. AU - Kim, Ki Rim. AU - Jeong, Chan Kwon. AU - Park, Kwang Kyun. AU - Choi, Jong Hoon. AU - Park, Jung Han Yoon. AU - Lim, Soon Sung. PY - 2010/5/6. Y1 - 2010/5/6. N2 - The anti-inflammatory activity of licorice (LE) and roated licorice (rLE) extracts determined in the murine phorbol ester-induced acute inflammation model and collagen-induced arthritis (CIA) model of human rheumatoid arthritis. rLE possessed greater activity than LE in inhibiting phorbol ester-induced ear edema. Oral administration of LE or rLE reduced clinical arthritis score, paw swelling, and histopathological changes in a murine CIA. LE and rLE decreased the levels of proinflammatory cytokines in serum and matrix metalloproteinase-3 expression in the joints. Cell proliferation and cytokine secretion in response to type II collagen or ...
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Genome-wide association studies have identified various susceptibility variants and loci associated with incidence of rheumatoid arthritis (RA) in different populations. One of these is T cell activation Rho GTPase activating protein (TAGAP). The present study sought to measure the expression of TAGAP in RA patients, CD4(+) T cells subsets from healthy humans and in mice with collagen-induced arthritis. Peripheral blood mononuclear cells (PBMC) from RA patients and tissues of arthritic mice at different stages of the disease were used for the evaluation of TAGAP mRNA expression. Increased TAGAP expression was observed in RA patients compared to healthy controls, and there were differences in the expression level of TAGAP in the tissues of mice with experimental arthritis. Gene expression in CD4(+) T cells from healthy humans was greatest 4 h after activation and protein expression was greatest after 24 h. The expression of TAGAP was not correlated with CD4(+) lymphocyte subsets which were enriched for
ASCs also promoted Treg generation in peripheral tissues, including dLNs, peritoneum, synovium, and peripheral blood. MSCs (ASCs) on in vitro formation of bone-resorbing osteoclasts and pathological bone loss in the mouse collagen-induced arthritis (CIA) model of RA. We observed that ASCs significantly inhibited receptor activator of NF-B ligand (RANKL)Cinduced osteoclastogenesis in both AMG 487 a contact-dependent and -independent manner. Additionally, ASCs inhibited RANKL-induced osteoclastogenesis in the presence of proinflammatory cytokines such as TNF-, IL-17, and IL-1. Furthermore, treatment with ASCs at the onset of CIA significantly reduced clinical symptoms and joint pathology. Interestingly, ASCs protected periarticular and systemic bone loss in CIA mice by maintaining trabecular bone structure. We further observed that treatment with ASCs reduced osteoclast precursors in bone marrow, resulting in decreased osteoclastogenesis. Moreover, ASCs suppressed autoimmune T cell responses and ...
OBJECTIVE: Endothelins (ETs) are involved in several inflammatory events. The present study investigated the efficacy of bosentan, a dual ETA/ETB receptor antagonist, in collagen-induced arthritis (CIA) in mice. TREATMENT: CIA was induced in DBA/1J mice. Arthritic mice were treated with bosentan (100 mg/kg) once a day, starting from the day when arthritis was clinically detectable. METHODS: CIA progression was assessed by measurements of visual clinical score, paw swelling and hypernociception. Histological changes, neutrophil infiltration and pro-inflammatory cytokines were evaluated in the joints. Gene expression in the lymph nodes of arthritic mice was evaluated by microarray technology. PreproET-1 mRNA expression in the lymph nodes of mice and in peripheral blood mononuclear cells (PBMCs) was evaluated by real-time PCR. The differences were evaluated by one-way ANOVA or Students t test. RESULTS: Oral treatment with bosentan markedly ameliorated the clinical aspects of CIA (visual clinical score,
TY - JOUR. T1 - Intra-articular injection of hyaluronate and indomethacin in rabbits with antigen-induced arthritis. AU - Lo, Yow Jen. AU - Sheu, Ming Thau. AU - Tsai, Wen Chi. AU - Lin, Yun Ho. AU - Li, Jau Le. AU - Liang, Yu Chih. AU - Chang, Chi Ching. AU - Hsieh, Ming-Shium. AU - Chen, Chien Ho. PY - 2007/10. Y1 - 2007/10. N2 - Combined effects of hyaluronate and indomethacin in the treatment of rabbits with antigen-induced arthritis (AIA) were evaluated by assessing joint swelling, C-reactive protein (CRP) and prostaglandin E2 (PGE 2) levels with periodic intra-articular (ia) injections of hyaluronate alone (HA group) and with either a low or high concentration of indomethacin (LI-HA or HI-HA group). End-point analyses included matrix metalloproteinases-3 (MMP-3) activity and macroscopic and histological joint examinations. Results demonstrated that treatment in LI-HA and HI-HA groups resulted in statistically significant suppression of CRP, PGE2, and MMP-3 in comparison with those of HA ...
Mast cells are abundantly expressed in synovial tissues and have been proposed to exert proinflammatory effects primarily based on antibody transfer-induced disease models (28, 29). The mode of mast cell activation and the mechanism by which activated-mast cells mediate antigen-induced arthritis are largely unknown and likely complex. We now provide direct in vivo evidence that IL-33 plays a major role in mast cell activation in the context of antigen-induced arthritis. Thus, IL-33 enhanced CIA when ST2−/− mice were reconstituted with BMMCs from WT but not from ST2−/− mice (Fig. 5). Our data also provide mechanisms by which mast cells could promote inflammatory synovitis (Fig. 6). IL-33 induces mast cell production of IL-1, IL-6, IL-13, and a range of chemokines (13, 14, 30) (Fig. 4). Because both IL-1 and IL-6 play crucial roles in the induction of Th17 cells (31-33), a key pathogenic cell type in arthritis (21, 22), our studies indicate a relationship between ST2/IL-33 function and ...
IL-17 is implicated in the pathogenesis of rheumatoid arthritis (RA) and has previously been shown to be induced by tumor necrosis factor (TNF) in vitro. The aim of this study was to assess the impact of TNF inhibition on IL-17 production in collagen-induced arthritis, a model of RA. TNF blockade using TNFR-Fc fusion protein or anti-TNF monoclonal antibody reduced arthritis severity but, unexpectedly, expanded populations of Th1 and Th17 cells, which were shown by adoptive transfer to be pathogenic. Th1 and Th17 cell populations were also expanded in collagen-immunized TNFR p55(-/-) but not p75(-/-) mice. The expression of IL-12/IL-23 p40 was up-regulated in lymph nodes (LN) from p55(-/-) mice, and the expansion of Th1/Th17 cells was abrogated by blockade of p40. Treatment of macrophages with rTNF also inhibited p40 production in vitro. These findings indicate that at least one of the ways in which TNF regulates Th1/Th17 responses in arthritis is by down-regulating the expression of p40. Finally,
Nimesulide is a COX-2 inhibitor used for symptomatic relief of rheumatoid arthritis. Leflunomide is an anti-pyrimidine used to manage the progression of rheumatoid arthritis. Herein we studied the influence of nimesulide and leflunomide combination in terms of disease symptoms and progression using collagen-induced arthritis model in mice, as a model for rheumatoid arthritis. Collagen induced arthritis was induced by immunization with type II collagen. Assessment of joint stiffness and articular hyperalgesia were evaluated using a locomotor activity cage and the Hargreaves method, respectively. Disease progression was assessed via arthritic index scoring, X-ray imaging, myeloperoxidase enzyme activity and histopathologic examination. Nimesulide induced only transient symptomatic alleviation on the top of decreased leucocytic infiltration compared to arthritis group. However, nimesulide alone failed to induce any significant improvement in the radiological or pathological disease progression. Leflunomide
Rheumatoid arthritis (RA) is a systemic autoimmune disease mediated by T cells. Collagen type II (CII) is one of the autoantigens associated with RA. CII263-272 is a predominant CII antigenic peptide that can induce T-cell activation upon binding to MHC and interaction with the appropriate T-cell receptor (TCR). Altered CII263-272 peptides with substitution of specific amino acids could bind to RA-associated HLA-DR4/1 with no T cell stimulating effects and could inhibit T cell activation in RA. We performed this study to evaluate the effect of mucosal administration and to explore the mechanism of the inhibitory effect of altered CII263-272 peptide (267Q --> A, 270K --> A and 271G --> A) on collagen induced arthritis (CIA). CIA was induced in Lewis rats by immunization with bovine CII. Altered CII263-272 peptide was given intranasally beginning from arthritis onset. Wild CII263-272 peptide or PBS was administered as controls. Therapeutic effects were evaluated by arthritis scores, body weight ...
TY - JOUR. T1 - Collagen-induced arthritis and TCRs in SWR and B10.Q mice expressing an E(α)/(k) transgene. AU - Griffiths, M. M.. AU - Nabozny, G. H.. AU - Hanson, J.. AU - Harper, D. S.. AU - McCall, S.. AU - Moder, K. G.. AU - Cannon, G. W.. AU - Luthra, H. S.. AU - David, C. S.. PY - 1994. Y1 - 1994. N2 - B10.E(α)/(k) transgenic mice were mated with H2-E- B10.Q and SWR mice. F1 and F1 x parental strain backcross progeny were tested for arthritis and autoimmune reactivity to mouse type II collagen (MII) after immunization with bovine, chick, deer, or human type II collagen. The results were correlated with the H-2 haplotype (b/q vs q/q) and the TCR Vβ profile of peripheral blood T cells in each mouse. Hybrid progeny expressed TCR profiles different from either parent because of the TCR Vβ genomic deletions of SWR mice (Vβa), the wild-type TCR allele of C57Bl/10 (B10) mice (Vβb), and the intrathymic negative selection processes resulting from cell surface expression of ...
Recently, therapy with bone marrow mesenchymal stem cells (BMMSCs) has been attempted to relieve rheumatoid arthritis (RA) and reconstruct cartilage injury. However, treatment has been unsuccessful in complete prevention of persistent cartilage destruction and resulted in inferior outcomes of cartilage regeneration. Scaffolds are an important construct in the field of cartilage tissue engineering, but their role in arthritis treatment has not yet been fully examined. Here, we transplanted two types of scaffold-assisted BMMSCs: fibrin gel- and poly(l-lactide-co-glycolide)−poly(ethylene glycol)−poly(l-lactide-co-glycolide) (PLGA−PEG−PLGA) hydrogel-assisted BMMSCs referred as FGB and HGB groups, respectively, into subchondral defects for the treatment of antigen-induced arthritis. The administration of exogenous BMMSCs ameliorated joint swelling and decreased both joint surface temperature and inflammatory cytokine levels in both groups. Immune cell composition of the inflammation of surrounding
Objectives: To establish if changes in Th1/Th17 cell populations previously reported in experimental arthritis occur in patients with rheumatoid arthritis (RA) treated with anti-tumour necrosis factor α (TNFα) agents, and whether the therapeutic response to anti-TNFα is compromised in patients and mice because of elevated Th17/IL-17 levels. Finally, to assess the efficacy of combined blockade of anti-TNFα and anti-IL-17 in experimental arthritis. Methods: A longitudinal study of two independent cohorts (cohort 1, n=24; cohort 2, n=19) of patients with RA treated with anti-TNFα biological agents was carried out to assess their Th17/IL-17 levels before and after the start of anti-TNFá therapy. IL-12/23p40 production was assessed in plasma Peripheral blood lymphocytes (PBLs) and monocytes. Mice with collagen-induced arthritis (CIA) were treated with anti-TNFα alone, anti-IL17 alone or a combination of the two. Efficacy of treatment and response was assessed from changes in Disease Activity Score 28
In countries where parasitic infections are endemic, autoimmune disease is relatively rare, leading to the hypothesis that parasite-derived immunomodulators may protect against its development. Consistent with this, we have previously demonstrated that ES-62, a 62 kDa phosphorylcholine (PC)-containing glycoprotein that is secreted by filarial nematodes, can exert anti-inflammatory action in the murine collagen-induced arthritis (CIA) model and human rheumatoid arthritis-derived synovial tissue cultures. As a first step to developing ES-62-based drugs, the aim of this study was to determine whether the PC-moiety of ES-62 was responsible for its anti-inflammatory actions. We compared the anti-inflammatory activity of a PC-free form of recombinant ES-62 (rES-62) and a synthetic PC-ovalbumin conjugate (OVA-PC) with that of native ES-62 in the CIA model and synovial tissues from patients with rheumatoid arthritis. Results: The anti-inflammatory actions of ES-62 in CIA appear to be dependent on the PC ...
article{418b5550-2321-4395-ab0c-946f90945ceb, abstract = {OBJECTIVE: To characterize the arthritis-modulating effects of 3 non-major histocompatibility complex (MHC) quantitative trait loci (QTLs) in rat experimental arthritis in the disease-resistant E3 strain, and to investigate the disease-modulating effects of the MHC region (RT1) in various genetic backgrounds. METHODS: A congenic fragment containing Ncf1 along with congenic fragments containing the strongest remaining loci, Pia5/Cia3 and Pia7/Cia13 on chromosome 4, were transferred from the arthritis-susceptible DA strain into the background of the completely resistant E3 strain. The arthritis-regulatory potential of the transferred alleles was evaluated by comparing the susceptibility to experimental arthritis in congenic rats with that in E3 rats. The RT1(u) haplotype from the E3 strain was transferred into the susceptible DA strain (RT1(av1)), and various F(1) and F(2) hybrids were generated to assess the effects of RT1 on arthritis ...
Background A long-term existing schistosome infection can certainly help in keeping immuno-homeostasis, thus providing protection against various types of autoimmune diseases to the infected sponsor. human rheumatoid arthritis. Illness by Schistosoma japonicum significantly reduced the severity and the incidence of experimental autoimmune collagen-induced arthritis. However, this beneficial effect can only be provided by a pre-established acute stage of illness but not by a pre-established early stage of the illness. The safety against collagen-induced arthritis correlated with reduced levels of anti-collagen II IgG, especially the subclass of IgG2a. Moreover, in safeguarded mice increased levels of IL-4 were present at the time of collagen II injection together with sustained higher IL-4 levels during the course of arthritis development. In contrast, in unprotected mice minimal levels of IL-4 were ITF2357 present at the initial stage of collagen II challenge together with lack of IL-4 induction ...
METHODS: Female wistar (Ws) rats were used in this study. CIA was induced by Native bovine type II collagen emulsified with complete Freunds adjuvant (CFA). Beta-END was administered i.p. to CIA rats every other day from the 14th day (secondary immunization) to the 35th day after primary immunization. Clinical assessments were performed by two independent, blinded examiners every other day. Pathological and radiological observations were taken on the 35th day after the primary immunization. Tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), regulated upon activation, normal T-cell expressed and secreted (RANTES), inducible NO syntheses (iNOS), matrix metalloproteinase-2 (MMP-2) and MMP-9 mRNA expression of synovium tissues of CIA rats was estimated by quantitative RT-PCR. The frequency of spleen Th1 and Th2 cells were assessed by fluorescence activated cell sorter (FACS) assay ...
Introduction: The Vbeta12-transgenic mouse was previously generated to investigate the role of antigen-specific T cells in collagen-induced arthritis (CIA), an animal model for rheumatoid arthritis. This mouse expresses a transgenic collagen type II (CII)-specific T-cell receptor (TCR) beta-chain and consequently displays an increased immunity to CII and increased susceptibility to CIA. However, while the transgenic Vbeta12 chain recombines with endogenous alpha-chains, the frequency and distribution of CII-specific T cells in the Vbeta12-transgenic mouse has not been determined. The aim of the present report was to establish a system enabling identification of CII-specific T cells in the Vbeta12-transgenic mouse in order to determine to what extent the transgenic expression of the CII-specific beta-chain would skew the response towards the immunodominant galactosylated T-cell epitope and to use this system to monitor these cells throughout development of CIA. Methods: We have generated and ...
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TY - JOUR. T1 - Myeloid DAP12-associating lectin (MDL)-1 regulates synovial inflammation and bone erosion associated with autoimmune arthritis. AU - Joyce-Shaikh, Barbara. AU - Bigler, Michael E.. AU - Chao, Cheng Chi. AU - Murphy, Erin E.. AU - Blumenschein, Wendy M.. AU - Adamopoulos, Iannis. AU - Heyworth, Paul G.. AU - Antonenko, Svetlana. AU - Bowman, Edward P.. AU - McClanahan, Terrill K.. AU - Phillips, Joseph H.. AU - Cua, Daniel J.. PY - 2010/3/15. Y1 - 2010/3/15. N2 - DNAX adaptor protein 12 (DAP12) is a trans-membrane adaptor molecule that transduces activating signals in NK and myeloid cells. Absence of functional Dap12 results in osteoclast defects and bone abnormalities. Because DAP12 has no extracelluar binding domains, it must pair with cell surface receptors for signal transduction. There are at least 15 known DAP12-associating cell surface receptors with distinct temporal and cell type-specific expression patterns. Our aim was to determine which receptors may be important in ...
B cells have a central role in the pathogenesis of collagen-induced arthritis (CIA), an animal model of the autoimmune disease rheumatoid arthritis. In this report, a specific subset of an innate type of B cells, B-1 B cells, have been studied for the involvement in CIA. The B-1 B cells were shown to produce small amounts of collagen-specific antibodies upon stimulation in vitro, suggesting that they play a minor role in the development of CIA. This report also includes how marginal zone B cells, another innate type of B cells with natural collagen-reactivity, can be identified in the medullary sinuses of lymph nodes of collagen-immunized mice, implying involvement in auto antigen trapping. ...
Y-320 is an orally active immunomodulator, and inhibits IL-17 production by CD4 T cells stimulated with IL-15 with IC50 of 20 to 60 nM. Y-320 also inhibits the production of IFN-γ and TNF-α by mouse CD4 T cells stimulated with IL-15, CXCL12, and anti-CD3 mAb. Y-320 (0.3-3 mg/kg p.o.) ameliorates collagen-induced arthritis (CIA) in Mice with a reduction of IL-17 mRNA in arthritic joints, and also shows therapeutic effects on CIA in cynomolgus monkeys. Moreover, Y-320 shows a synergistic effect in combination with anti-TNF-α mAb on chronic-progressing CIA in mice. Selleck Chemicals Y-320, IL-17 Inhibitor. 26 Sep 2014. Ushio H, et al. (2013). A new phenylpyrazoleanilide, y-320, inhibits interleukin 17 production and ameliorates collagen-induced arthritis in mice and cynomolgus monkeys. Pharmaceuticals (Basel). 7 (1): 1-17. doi:10.3390/ph7010001. PMC 3915191 . PMID 24366113 ...
Therapeutic Treatment of Arthritic Mice with 15-Deoxy Δ12,14-Prostaglandin J2 15d-PGJ2 Ameliorates Disease through the Suppression of Th17 Cells and the Induction of CD4 CD25−FOXP3 Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Rheumatoid arthritis progression has been markedly improved by the application of biological anti-RA agents, which suppress RA activity and also repair affected joint space under certain conditions.
Elevated expression of LL-37 and its activating protease have been described in RA patients (6, 64), and mechanistic studies are beginning to unravel the role of LL-37 in this disease. Periarticular osteopenia is a common finding among patients with RA (65), and LL-37 induces apoptosis of osteoblasts, which could contribute to reduced bone formation in arthritic joints (7). Furthermore, a recent report characterized elevated expression of LL-37 primarily in the osteoclasts and granulocytes within the human RA synovium (66). Using a pristane-induced arthritis model in rats, upregulation of rCRAMP, the rat ortholog of LL-37, was demonstrated in granulocytes, macrophages, and γδ T cells of synovial fluid. Importantly, transfer of pristane-exposed neutrophils induced arthritis, whereas type I IFN or autoantibody responses in control rats did not (66). This suggests that LL-37 may contribute to arthritis development, but further studies are needed to clarify its role.. An association between ...
Phasic changes in the immune response were observed in rats and mice with adjuvant disease: stimulation of antibody formation on the seventh day after injection of Freunds complete adjuvant (FCA)...
Doctors use the rheumatoid arthritis severity scale to track the level and progress of your disease. Find out what it measures and what your numbers mean.
We have now previously reported a rise in interleukin (IL)-oneβ and IL-17 amounts, along with a ongoing activation of caspase-1 in early rheumatoid arthritis (RA) patients. These success recommend that medications concentrating on IL-1β regulatory pathways, Together with tumor necrosis variable (TNF), may perhaps constitute promising therapeutic agents in early RA. We have just lately utilized a THP-one macrophage-like mobile line to screen 2320 compounds for those who down-regulate both of those IL-1β and TNF secretion. Celastrol was one of the most promising therapeutic candidates determined in that review. Our major aim within the existing get the job done was to research no matter whether administration of celastrol can attenuate inflammation in a very rat design of adjuvant-induced arthritis (AIA). Additionally, since IL-1β is thought to Perform a task within the polarization of Th17 cells, we also investigate irrespective of whether administration of digoxin, a specific inhibitor of ...
The application of tumor necrosis factor inhibitors (TNFi) is a major breakthrough in the treatment of rheumatoid arthritis (RA). While the anti-inflammatory nature of TNFi is thought to contribute to the therapeutic effects, recent data show that the pharmacology of TNF- blockade is probably...
The arthritis-susceptible DA rat is one of the most commonly used rat strains for genetic linkage analysis and is instrumental for the identification of many genetic loci. Even though DA rats were kept as inbred lines at different institutes and suppliers, it became obvious that the various breeding …
Background The systems underlying tolerance maintenance and induction in autoimmune arthritis stay elusive. to na?ve rodents ameliorated the advancement of CII-induced joint disease. Bottom line Our data recommend that endogenous display of the CII-peptide on N cells can be one of the essential members to joint disease patience induction and maintenance. Electronic ancillary materials The online edition of this content (doi:10.1186/s13075-016-1037-7) contains supplementary materials, which is obtainable to authorized users. IgG ELISA Heat-killed L37Ra (Difco,?BD Biosciences,?Franklin Ponds, New Shirt,?USA) 0.4?mg/ml was dissolved in carbonate barrier, and filtered through a 22?m Millipore filtration system. A 96-well dish (Nunc Maxisorp) was covered with 100?d per good of the option and incubated in 4?C further and overnight blocked with PBS with BSA 1?%, Tween 1?%. The serum was serially diluted from 1:8 to 1:512 and the dish was incubated at 4?C overnight. Biotinylated goat anti-mouse IgG ...
Stem cell-derived tissue- associated regulatory T cells ameliorate the development of autoimmune arthritis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
OBJECTIVE: We studied histological changes in the cell populations of subchondral bone marrow resulting from inflammation in animal models of arthritis. Inflammation in intraosseous spaces and the effect of treatment with the aminobisphosphonate zoledronate were assessed. METHODS: Peripheral blood, femoral bone marrow, and spleen cells were harvested from carrageenan-induced arthritis Sprague-Dawley rats; fluorescence stained for CD3, CD8, CD4, CD11b/c, anti-mononuclear phagocyte (MNP), CD3/CD8, CD3/CD4, and MNP/CD11b/c; and evaluated by flow cytometry. Arthritis was induced in New Zealand white rabbits in 5 treatment groups: normal, arthritis, zoledronate-treated from arthritis induction, or 2 or 4 weeks after induction. Animals were euthanized after 7 weeks and distal femoral condyles were decalcified, processed, sectioned, and stained. Sections were evaluated for several cell types and histological features relevant to inflammation, which were assigned a categorical grade from 0 (healthy ...
Delayed type hypersensitivity. On Day 1 of the study, mice were IV injected with washed sheep red blood cells (SRBC). On Day 6 of the study, mice were treated with either vehicle or Dexamethasone followed by injection of SRBCs into the paws. Injection of SRBCs into the paws of sensitized mice produced an allergic reaction as measured by swelling (paw thickness). Dexamethasone, a steroid anti-inflammatory, significantly reduced footpad swelling as measured using a microcaliper. Data are mean ± SEM; ***p,0.001 compared to vehicle (N=7-8).. The mouse DTH model is a test against an immunological inflammatory response. Animals that received vehicle treatment displayed an increased paw thickness compared to animals administered Dexamethasone, providing an immunological benefit. These data demonstrate a valid methodology for producing an allergic reaction in experimental animals and show that these animals are sensitive to pharmacological agents that are known to reduce allergic reactions.. ...
The other day I was talking with my aunt about her severe arthritis. She kept mentioning that her hands and knees were really bothering her and no matter what she tried, she could get no relief.
University of Melbourne researchers have identified a protein involved in rheumatoid arthritis-induced inflammation that could lead to new drug treatments.
The importance of the BCR and TLR9 in autoimmunity and in the production of autoantibodies is well established but the underlying molecular mechanism still needs to be determined. Here we aim to characterize the BCR-TLR9 crosstalk by its effect on T-bet, as T-bet is activated and regulated by both receptors and has an important role in class-switching to pathological IgG2a in mice. Using primary mouse B cells, we demonstrate that T-bet expression is synergistically elevated by the crosstalk between the BCR and TLR9. To test the effect of this synergy on IgG2a-switching, the levels of switched B cells were checked by functional tests. We found that BCR co-stimulation had no additional effect on TLR9-induced IgG2a expression however the expression of Rad51 was synergistically increased. To check the biological significance of the synergy we compared T-bet expression in B cells from healthy and CIA (collagen-induced arthritis) mice but no differences were found. Taken together, we demonstrate here ...
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WASHINGTON - Some of the worlds largest drugmakers will face an uphill battle next week in their bid to revive a class of experimental arthritis drugs that have been sidelined by safety concerns for nearly two years.. The Food and Drug Administration says there is a clear association between the nerve-blocking medications and incidences of joint failure that led the agency to halt studies of the drugs in 2010. However, the agency also notes that those side effects were less common when the drugs were used at lower doses, potentially leaving the door open for future use. The agency released its safety analysis ahead of a public meeting next week where outside experts will discuss the drugs safety.. On Monday, Pfizer Inc., Johnson & Johnson and Regeneron Pharmaceuticals will make their case to continue studies of the drugs, with safety precautions to protect patients.. The request to restart testing is unusual, since drugmakers often abandon research on experimental drugs that appear to have ...
New data raising safety concerns for Pfizer Incs experimental arthritis drug sent its shares down 3 percent, while lifting shares of rival drugmaker Abbott Laboratories, analysts said.
Clinical and Experimental Rheumatology. 28 (3): 333-40. PMID 20406616. Harrison's Rheumatology, Second Edition [Anthony Fauci, ... Reactive arthritis, also known as Reiter's syndrome, is a form of inflammatory arthritis that develops in response to an ... Arthritis occurring alone following sexual exposure or enteric infection is also known as reactive arthritis. Patients can also ... "Arthritis and Rheumatism". Retrieved 16 May 2011. eMedicine/Medscape (5 January 2010). "Reactive Arthritis". Retrieved 16 May ...
Isaacs D (2010-07-07). "Infectious risks associated with biologics". Advances in Experimental Medicine and Biology. 764: 151- ... Wikimedia Commons has media related to Rheumatoid arthritis. Rheumatoid arthritis at Curlie "Rheumatoid Arthritis". MedlinePlus ... "Pain management for inflammatory arthritis (rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis and other ... in people receiving methotrexate for inflammatory arthritis (rheumatoid arthritis, ankylosing spondylitis, psoriatic arthritis ...
Clinical and Experimental Rheumatology. 16 (3): 277-281X. PMID 9631749. "Post Traumatic Wrist Arthritis , Orthopaedic Surgery ... such as rheumatoid arthritis or psoriatic arthritis. The symptoms of post-traumatic arthritis are similar to the ones occurring ... Post-traumatic arthritis (PTA) is a form of osteoarthritis following an injury to a joint. Post-traumatic arthritis is a form ... Since post-traumatic arthritis usually occurs after injuring a joint, the risk of having post-traumatic arthritis after such an ...
Advances in Experimental Medicine and Biology. Vol. 764. pp. 151-8. doi:10.1007/978-1-4614-4726-9_12. ISBN 978-1-4614-4725-2. ... Arthritis mutilans has also been called chronic absorptive arthritis, and may be seen in rheumatoid arthritis as well. ... reactive arthritis, gouty arthritis, systemic lupus erythematosus, and inflammatory bowel disease-associated arthritis. In ... Psoriatic arthritis is a long-term inflammatory arthritis that occurs in people affected by the autoimmune disease psoriasis. ...
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Unlike rheumatoid arthritis, lupus arthritis is less disabling and usually does not cause severe destruction of the joints. ... There is no cure for SLE, but there are experimental and symptomatic treatments. Treatments may include NSAIDs, corticosteroids ... Arthritis: nonerosive arthritis of two or more peripheral joints, with tenderness, swelling, or effusion; sensitivity = 86%; ... Fewer than ten percent of people with lupus arthritis will develop deformities of the hands and feet. People with SLE are at ...
However, about one third of UCTD patients will differentiate to a specific autoimmune disease, like rheumatoid arthritis or ... Clinical and Experimental Rheumatology. 22 (3 Suppl 33): S14-S18. PMID 15344591. Bodolay E, Csiki Z, Szekanecz Z, Ben T, Kiss E ... 2008). "Vitamin D deficiency in undifferentiated connective tissue disease". Arthritis Research & Therapy. 10 (5): R123. doi: ... Clinical and Experimental Rheumatology. 21 (3): 313-320. PMID 12846049. Zold E, Szodoray P, Gaal J, Kappelmayer J, Csathy L, et ...
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The joints involved tend to be the ankles, knees, and elbows, but arthritis in the hands and feet is possible; the arthritis is ... 2002). "Problems of classification of Henoch Schonlein purpura: an Indian perspective". Clinical and Experimental Dermatology. ... Purpura, arthritis, and abdominal pain are known as the "classic triad" of Henoch-Schönlein purpura. Purpura occur in all cases ... Schönlein associated the purpura and arthritis, and Henoch the purpura and gastrointestinal involvement. The English physician ...
... , sold under the brand Xeljanz among others, is a medication used to treat rheumatoid arthritis, psoriatic arthritis ... Herper M (2 March 2011). "Why Pfizer's Biggest Experimental Drug Got A Name Change". Forbes. Retrieved 3 March 2011. Moisan A, ... FDA approves Boxed Warning about increased risk of blood clots and death with higher dose of arthritis and ulcerative colitis ... "FDA approves Boxed Warning about increased risk of blood clots and death with higher dose of arthritis and ulcerative colitis ...
Proposal of an international consensus conference; Arthritis Rheum 2013 Jennette, JC; Overview of the 2012 Revised ... International Chapel Hill Consensus Conference Nomenclature of Vasculitides; Clinical and Experimental Nephrology, 2013 ( ...
Job-Deslandre C (January 2007). "Idiopathic juvenile-onset systemic arthritis". Orphanet. Orphan number: ORPHA85414. Amaru ... Experimental Hematology. 40 (8): 634-45.e10. doi:10.1016/j.exphem.2012.04.007. PMID 22579713. (Drugs not assigned an ATC code, ... and has been granted orphan drug designation in the European Union for the treatment of systemic juvenile idiopathic arthritis ... Givinostat for the treatment of systemic-onset juvenile idiopathic arthritis" (PDF). European Medicines Agency. Retrieved 2010- ...
Specifically, individuals with rheumatoid arthritis and Sjögren syndrome have increased rates of bronchiectasis. In these ... Clinical and Experimental Allergy. 43 (8): 850-873. doi:10.1111/cea.12141. PMID 23889240. S2CID 24077597. Wilczynska, Maria M ... Chatzidionisyou, Aikaterini; Catrina, Anca I. (January 2016). "The lung in rheumatoid arthritis, cause or consequence?". ... The Tohoku Journal of Experimental Medicine. 222 (4): 237-242. doi:10.1620/tjem.222.237. PMID 21127394. Kim, C. K.; Chung, C. Y ...
Targoff, IN; Reichlin, M (1985). "Nucleolar localization of the PM-Scl antigen". Arthritis & Rheumatism. 28 (2): 226-30. doi: ... Clinical and Experimental Immunology. 81 (1): 59-64. doi:10.1111/j.1365-2249.1990.tb05291.x. PMC 1535032. PMID 2199097. ... 2002). "Autoantibodies directed to novel components of the PM/Scl complex, the human exosome". Arthritis Research & Therapy. 4 ... arthritis, myositis and scleroderma. Treatment of these patients is symptomatic and is similar to treatment for the individual ...
The Effect of Salicylates on Experimental Arthritis in Rabbits. Chicago: American Medical Association, 1917. Fantus, Bernard. ... to strengthen New Jersey's competitive position for business and industry The effect of salicylates on experimental arthritis ... Tolu and sugar coating in the disguising of medicines Tungstates of alkaloids-An experimental pharmacologic study X‐Ray ... "Tungstates of alkaloids-An Experimental Pharmacologic Study." The Journal of Laboratory and Clinical Medicine 3, no. 3 (1917): ...
"Novel suppressive function of transitional 2 B cells in experimental arthritis". Journal of Immunology. 178 (12): 7868-78. doi: ... By contrast mouse Bregs in model of collagen-induced arthritis (CIA) were mainly CD21 and CD23 positive. Breg were found in ... Then a role of Bregs was found in many mouse models of autoimmune diseases as rheumatoid arthritis or systemic lupus ... An increase in expression of this molecule has been described in the collagen-induced arthritis model. In order to suppress ...
"The oncoprotein TBX3 is controlling severity in experimental arthritis". Arthritis Research & Therapy. 21 (1): 16. doi:10.1186/ ... KLF12 as a risk locus for rheumatoid arthritis susceptibility". Arthritis and Rheumatism. 58 (8): 2275-86. doi:10.1002/art. ... TBX3 has been implicated in human diseases including the ulnar mammary syndrome, obesity, rheumatoid arthritis and cancer. In ... Genome wide association studies also causally linked TBX3 to rheumatoid arthritis (RA) susceptibility and a recent study ...
Experimental studies have shown that certain mutated viruses may be able to trigger JIA. The disease appears to be more common ... "Is juvenile rheumatoid arthritis/juvenile idiopathic arthritis different from rheumatoid arthritis?". Arthritis Research. 4 ( ... Arthritis Australia, Juvenile Idiopathic Arthritis [email protected] (UK) JIA - NIH Medline Plus. (Articles with short description, ... JIA is a subset of childhood arthritis, but unlike other, more transient forms of childhood arthritis, JIA persists for at ...
May 2017). "Response of human rheumatoid arthritis osteoblasts and osteoclasts to adiponectin". Clinical and Experimental ... Adiponectin is a protein hormone that has been shown to be upregulated in rheumatoid arthritis disease pathology, causing the ... bone growth by osteoblasts is a partial cause of the abnormal degradation of bone seen in osteoporosis or rheumatoid arthritis ...
"Serum procalcitonin for discrimination between septic and non-septic arthritis". Clinical and Experimental Rheumatology. 26 (3 ... The study also concluded that PCT outperforms C-reactive protein in differentiating septic arthritis from non-septic arthritis ... PCT at a cutoff value of .5 ng/mL was effective at ruling in septic arthritis in an analysis of over 8000 patients across 10 ... May 2005). "Diagnostic value of serum and synovial procalcitonin in acute arthritis: a prospective study of 42 patients". ...
"Dead Sea bath salts for the treatment of rheumatoid arthritis". Clinical and Experimental Rheumatology. 8 (4): 353-357. PMID ... Researchers have also studied their use in treating arthritis. Bath fizzies are material products designed to effervesce in ...
Advances in Experimental Medicine and Biology. Vol. 735. pp. 55-81. doi:10.1007/978-1-4614-4118-2_4. ISBN 978-1-4614-4117-5. ... Arthritis and Rheumatism. 30 (9): 961-6. doi:10.1002/art.1780300901. PMID 2959289. Hourcade D, Miesner DR, Atkinson JP, Holers ... The Journal of Experimental Medicine. 173 (5): 1159-63. doi:10.1084/jem.173.5.1159. PMC 2118866. PMID 1708809. Khera R, Das N ( ... The Journal of Experimental Medicine. 168 (5): 1699-717. doi:10.1084/jem.168.5.1699. PMC 2189104. PMID 2972794. Hing S, Day AJ ...
... a unique inflammatory cytokine with roles in bone biology and arthritis". Arthritis Research & Therapy. 6 (6): 240-7. doi: ... In experimental pneumonia models, IL-17A or IL-17RA knock mice have increased susceptibility to various Gram-negative bacteria ... Lubberts E, Koenders MI, van den Berg WB (2006). "The role of T-cell interleukin-17 in conducting destructive arthritis: ... Th17 cells is also strongly associated rheumatoid arthritis (RA), a chronic disorder with symptoms include chronic joint ...
... rheumatoid arthritis, and psoriatic arthritis. Squamous cell carcinoma has been found on rare occasions in chronic hidradenitis ... Clinical and Experimental Rheumatology. 2 (4): 309-11. PMID 6241861. Velpeau A. Aissele. In: Bechet Jeune Z: Dictionnaire de ... Schneeweiss MC, Kim SC, Schneeweiss S, Rosmarin D, Merola JF (2020). "Risk of inflammatory arthritis after a new diagnosis of ... Vasey FB, Fenske NA, Clement GB, Bridgeford PH, Germain BF, Espinoza LR (1984). "Immunological studies of the arthritis of acne ...
Hilkens CM, Isaacs JD (May 2013). "Tolerogenic dendritic cell therapy for rheumatoid arthritis: where are we now?". Clinical ... The Journal of Experimental Medicine. 195 (1): 15-21. doi:10.1084/jem.20011341. PMC 2196016. PMID 11781361. Monney L, Sabatos ... and Experimental Immunology. 172 (2): 148-57. doi:10.1111/cei.12038. PMC 3628318. PMID 23574312. Mannie MD, Curtis AD (May 2013 ... regulatory T cells play a critical role in granulocyte-macrophage colony-stimulating factor-induced suppression of experimental ...
The effect of D-penicillamine on experimental allergic arthritis in rabbits]". Z Rheumatol. 35 (5-6): 201-9. PMID 60013. ...
... intradermally in diabetes and rheumatoid arthritis patients, subcutaneously in rheumatoid arthritis patients and via ... The Journal of Experimental Medicine. 192 (9): 1213-22. doi:10.1084/jem.192.9.1213. PMC 2193357. PMID 11067871. Lim DS, Kang MS ... Hilkens, C M U; Isaacs, J D (May 2013). "Tolerogenic dendritic cell therapy for rheumatoid arthritis: where are we now?". ... Hilkens, C. M. U.; Isaacs, J. D. (2013-04-10). "Tolerogenic dendritic cell therapy for rheumatoid arthritis: where are we now ...
... -dependent toll-like receptor 9 signaling revealed in experimental arthritis. Science, 319(5863), 624-627. Hussein, ...
... psoriatic arthritis, Behçet's disease, arthritis, inflammatory bowel disease (IBD), and allergy, as well as many cardiovascular ... Advances in Experimental Medicine and Biology). Springer, 2007. idid.us: immune mediated inflammatory diseases, inflammatory ... Immune mediated polygenic arthritis Shurin, Michael R. and Yuri S. Smolkin (editors). Immune Mediated Diseases: From Theory to ...
Achterberg received her Ph.D.in General Experimental Psychology from Texas Christian University, was a faculty member for 11 ... "The psychological dimensions of arthritis." Journal of Consulting and Clinical Psychology, Vol. 50, No. 6, 1982, p984. Gibbs, ... Multivariate Experimental Clinical Research, 1977. Walach, H., Jean Achterberg Obituary. Forschende Komplementärmedizin, Vol. ...
An experimental geothermal energy plant has also been set up here. Manikaran is a pilgrimage centre for Hindus and Sikhs. The ... Bathing here is known as a balm for arthritis. This temple is hugely revered as it belongs to Lord Shiva. However, an ...
... controlling the CMTM5 gene in the blood of individuals with the autoimmune disease of rheumatoid arthritis are hypo-methylated ... by in silico cloning and experimental validation". Genomics. 81 (6): 609-17. doi:10.1016/s0888-7543(03)00095-8. PMID 12782130. ...
Several experimental and clinical studies have indicated that aberrations and deregulations of the UPS contribute to the ... Lastly, autoimmune disease patients with SLE, Sjögren syndrome and rheumatoid arthritis (RA) predominantly exhibit circulating ...
"Caspase-activated DNase Is Required for Maintenance of Tolerance to Lupus Nuclear Autoantigens." Arthritis and Rheumatism 64.4 ... and manipulation of purified DNA fragment There are numerous experimental variations to this scheme, but these steps are ...
Kalliolias GD, Ivashkiv LB (2010). "Overview of the biology of type I interferons". Arthritis Research & Therapy. 12 Suppl 1 ( ... The Journal of Experimental Medicine. 137 (2): 317-30. doi:10.1084/jem.137.2.317. PMC 2139494. PMID 4346649. Tan YH (March 1976 ...
Bostock was often experimental in his approach to technique. Economic as well as innovative considerations led him to use a ... Sadly, by 1988, deteriorating eyesight and the onset of arthritis caused him to cease wood engraving - although, even then, he ... Some blocks that were experimental, or to be used commercially, were not editioned. About five to ten proofs were printed from ...
Experimental production of mice deprived of selected T-cell subclasses. J. Exp. Med. 1976;144:1128. Nabel G, Fresno M, Chessman ... Proc Natl Acad Sci USA 2011;108:14584 Leavenworth JW, Tang X, Kim HJ, Wang X, Cantor H. Amelioration of arthritis through ... This experimental approach was also used to investigate other lymphocyte populations, including "natural killer" cells. His ... Mobilization of natural killer cells inhibits development of collagen-induced arthritis. ...
He invented a portable, battery-operated lift seat for people suffering from knee arthritis. Rodriguez was the leader of the ... She is also responsible for planning experimental studies to validate analytical techniques and study solar sails dynamics. ... Her first assignment being as a project officer at the National Institute of Arthritis, Metabolism and Digestive Diseases of ... battery-operated lift seat for people suffering from knee arthritis; Felix Soto Toro developed the Advanced Payload Transfer ...
... arthritis; spinal cord, brain, and lung injury; injury due to ischemia in the heart, brain, kidney, and gut; and stress-induced ... The Journal of Pharmacology and Experimental Therapeutics. 306 (2): 463-70. doi:10.1124/jpet.103.050955. PMID 12721327. S2CID ...
Based on experimental studies and clinical experience Aaseth recommend DMSA to be used against mercury and lead intoxications, ... 1998). Rheumatoid arthritis and metal compounds-perspectives on the role of oxygen radical detoxification. Analyst, 123(1), 3-6 ... Experimental data demonstrate that Se treatment modifies brain Hg retention, modulates neurotoxicity and oxidative stress in ... The Journal of Trace Elements in Experimental Medicine: The Official Publication of the International Society for Trace Element ...
... an epidemic of oligoarticular arthritis in children and adults in three Connecticut communities". Arthritis and Rheumatism. 20 ... Ercolini AM, Miller SD (January 2009). "The role of infections in autoimmune disease". Clinical and Experimental Immunology. ... People with Lyme arthritis should limit their level of physical activity to avoid damaging affected joints, and in case of ... Lyme arthritis occurs in up to 60% of untreated people, typically starting about six months after infection. It usually affects ...
Pratt WB, Morishima Y, Murphy M, Harrell M (2006). "Chaperoning of glucocorticoid receptors". Handbook of Experimental ... Chikanza IC (June 2002). "Mechanisms of corticosteroid resistance in rheumatoid arthritis: a putative role for the ... Experimental Cell Research. 247 (2): 461-474. doi:10.1006/excr.1998.4375. PMID 10066374. Kanelakis KC, Shewach DS, Pratt WB ( ... Experimental Cell Research. 201 (1): 99-108. doi:10.1016/0014-4827(92)90352-9. PMID 1612132. Hurley DM, Accili D, Stratakis CA ...
Several experimental and clinical studies have indicated that aberrations and deregulations of the UPS contribute to the ... Lastly, autoimmune disease patients with SLE, Sjögren syndrome and rheumatoid arthritis (RA) predominantly exhibit circulating ...
Research applications include transplantation research, induced arthritis and inflammation, experimental allergic encephalitis ... The reproductive function of rats was studied at the Institute for Experimental Biology at the University of California, ... Krinke GJ, Bullock GR, Krinke G (June 15, 2000). "History, Strains and Models". The Laboratory Rat (Handbook of Experimental ... I. Demonstration of a defect in phagocytosis". The Journal of Experimental Medicine. 124 (2): 227-40. doi:10.1084/jem.124.2.227 ...
Examples include methotrexate (used in chemotherapy and in treating rheumatic arthritis) and the protease inhibitors used to ... which are then followed by experimental confirmation of binding of the virtual screening hits. Complementary approaches that ... inflammatory cytokines and hence these inhibitors are used to treat a variety of inflammatory diseases in including arthritis, ...
Patients with rheumatoid arthritis (RA) have been found to make autoantibodies toward the carbamylated form of A1AT in the ... Experimental Immunology. 179 (2): 161-172. doi:10.1111/cei.12476. PMC 4298394. PMID 25351931. Winkler IG, Hendy J, Coughlin P, ... The Journal of Experimental Medicine. 201 (7): 1077-88. doi:10.1084/jem.20042299. PMC 2213124. PMID 15795238. Richler R, ... as an antigenic target of anti-CarP antibodies in patients with rheumatoid arthritis". Journal of Autoimmunity. 80: 77-84. doi: ...
Experimental and experimental vibrational spectroscopy as a probe of structure, function and mechanisms in complex biological ... "A Rheumatoid arthritis study using Raman spectroscopy". Theoretical Chemistry Accounts. 130 (4-6): 1211-1220. doi:10.1007/ ...
Among other comments, the FDA noted the poor experimental design of some trials. In 2007, Reichenbach et al. used explicit ... "Glucosamine/Chondroitin Arthritis Intervention Trial (GAIT)," ClinicalTrials.gov information on the purpose, design, and ... Palmieri L, Conte A, Giovannini L, Lualdi P, Ronca G. Metabolic fate of exogenous chondroitin sulfate in the experimental ... Pharmacokinetic studies performed on humans and experimental animals after oral administration of chondroitin sulfate revealed ...
Advances in Experimental Medicine and Biology. 455: 85-92. doi:10.1007/978-1-4615-4857-7_12. ISBN 978-1-4613-7203-5. PMID ... The signs to look for include Raynaud's phenomenon, arthritis, myositis and scleroderma. Visual symptoms include discoloring of ... arthritis, myositis and scleroderma. Treatment of these patients is therefore strongly dependent on the exact symptoms with ... Clinical and Experimental Rheumatology. 24 (2): 129-33. PMID 16762146. Saw, Jacqui; Leong, Wai; John, Mina; Nolan, David; ...
The Romanian Society of Gerontology was the first in the world to channel its research into clinic, experimental, and social ... While experimenting on the effects that procaine had on arthritis, Aslan discovered other beneficial effects of this drug. It ...
... used to relieve pain and treat arthritis. At the time of her death from cancer on 9 November 2020 aged 61, she was Professor of ... Journal of Pharmacology and Experimental Therapeutics. 312 (3): 1206-1212. doi:10.1124/jpet.104.076877. ISSN 0022-3565. PMID ... for the treatment of pain and arthritis. In her subsequent academic career at Washington University, Seibert published evidence ...
But, when the arthritis is an isolated symptom, it is referred to as poststreptococcal reactive arthritis. This arthritis can ... Journal of Experimental Medicine. 119 (5): 761-780. doi:10.1084/jem.119.5.761. PMC 2137738. PMID 14157029. Krause, R. M. (2002 ... Poststreptococcal reactive arthritis: The presentation of arthritis after a recent episode of group A streptococcal pharyngitis ... These criteria include arthritis, carditis, neurological issues, and skin findings. Diagnosis also depends on evidence of a ...
White WB (May 2009). "Defining the problem of treating the patient with hypertension and arthritis pain". The American Journal ... Lupkin, Sydney (24 March 2020). "FDA Grants Experimental Coronavirus Drug Benefits For Rare Disease Treatments". NPR. Archived ... was developed by Paul Ehrlich and chemist Alfred Bertheim of the Institute of Experimental Therapy in Berlin. The drug was ... an anti-inflammatory medication used for arthritis. In the Bextra case, the government also charged Pfizer with illegally ...
Animal models of experimental colitis and pulmonary hypertension indicate a protective effect as well. Two studies have ... Jacob S (2003). MSM the Definitive Guide: Nutritional Breakthrough for Arthritis, Allergies and More. Freedom Press. ISBN 978-1 ... Amirshahrokhi K, Bohlooli S, Chinifroush MM (June 2011). "The effect of methylsulfonylmethane on the experimental colitis in ... "MSM for arthritis pain: Is it safe?". Expert Answers. Mayo Clinic. Retrieved 14 July 2015. Usha PR, Naidu MU (2004). " ...
In experimental models, dendritic cells have also been shown to contribute to the success of cancer immunotherapies, for ... Arthritis Res. 4 Suppl 3 (Suppl 3): S127-32. doi:10.1186/ar567. PMC 3240143. PMID 12110131. McKenna K, Beignon A, Bhardwaj N ( ... The Journal of Experimental Medicine. 137 (5): 1142-1162. doi:10.1084/jem.137.5.1142. PMC 2139237. PMID 4573839. Banchereau J, ...
... and rheumatoid arthritis to PMS. A 2005 meta-analysis of clinical trials that had been conducted at that time, found that the ... Experimental psychology, Devices to alter consciousness). ...
... of capillary permeability in inflammation and allergic responses as well as partial suppression of experimental arthritis in ... activation is critical for the development of arthritis in different animal models. PGF2α: FP-(PGF2α) (PTGFR) - PTGFR; FP is ...
Autoantibodies to CA3 have been found to be significantly higher in patients with rheumatoid arthritis, systemic lupus ... Journal of Neuropathology and Experimental Neurology. 46 (4): 451-60. doi:10.1097/00005072-198707000-00004. PMID 3110380. S2CID ... "Carbonic anhydrases III and IV autoantibodies in rheumatoid arthritis, systemic lupus erythematosus, diabetes, hypertensive ...
Experimental evidence shows that there is substantial increase of ceramide levels upon adding palmitate. Ceramide accumulation ... Most of the studies on S1P are used to further understand diseases such as cancer, arthritis and inflammation, diabetes, immune ...
Unger, D. L. (1998). "Does knuckle cracking lead to arthritis of the fingers?". Arthritis & Rheumatism. 41 (5): 949-950. doi: ... Experimental study". European Urology. 24 (3): 375-80. doi:10.1159/000474332. PMID 8262106. Shafik, Ahmed (May 1992). " ... Medicine: Donald L. Unger of Thousand Oaks, California, US, for investigating a possible cause of arthritis of the fingers, by ... for his experimental conclusion that 4,000 trained meditators caused a 24 percent decrease in violent crime in Washington, D.C ...
Conclusions: Our findings shed light on the therapeutic efficacy of pyrimethaminein experimental rheumatoid arthritis compared ... The present study was designed to test the therapeuticeffect of pyrimethamine (PYR) in collagen-induced arthritis (CIA). ... with a choice drug (methotrexate), which may recommendedit as a second-line drug in the treatment of rheumatoid arthritis. ... Effect of pyrimethamine in experimental rheumatoid arthritis. Med Sci Monit 2005; 11(8): BR293-299 :: ID: 201062 ...
Our findings highlight that TNFα stimulates autophagy in osteoclasts in both experimental arthritis and RA. Of note, other ... In our study, we further demonstrate that inhibition of autophagy prevented joint destruction in experimental arthritis. ... Zoledronic acid protects against local and systemic bone loss in tumor necrosis factor-mediated arthritis. Arthritis Rheum 2004 ... Identification of cell types responsible for bone resorption in rheumatoid arthritis and juvenile rheumatoid arthritis. Am J ...
Arthritis was induced in New Zealand white rabbits in 5 treatment groups: normal, arthritis, zoledronate-treated from arthritis ... In arthritis, these cell types and tissues displayed a shift of 1-2 grades higher (toward inflammation). Intact tidemark was ... with intraosseous inflammation in the subchondral bone marrow of joints affected by experimental inflammatory arthritis and is ... with intraosseous inflammation in the subchondral bone marrow of joints affected by experimental inflammatory arthritis and is ...
Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease of unknown cause. The hallmark feature of this condition ... Experimental therapies. Despite significant advances over the past decades, RA continues to be a chronic disease. It remains ... Chapter 5: Ultrasound of Rheumatoid Arthritis. Bruno MA, Mosher TJ, Gold GE. Arthritis in Color: Advanced Imaging of Arthritis ... Risk of septic arthritis in patients with rheumatoid arthritis and the effect of anti-TNF therapy: results from the British ...
The most common medical conditions were arthritis/chronic pain (42.9%), hypertension (33.1%), hepatitis/cirrhosis (25.3%), and ... Although the quasi-experimental study design is an improvement over the design of previous studies, unmeasured differences ... We conducted a prospective, multicenter, quasi-experimental, single-blinded study at 2 VHA medical centers to assess health ... This was a prospective, multicenter, quasi-experimental, single-blinded trial that compared patterns of health care service use ...
This is the first study in which the effects of E2 on Th17 cells have been characterized in experimental autoimmune arthritis. ... The aim of this study was to investigate the effects of estrogen on Th17 cells in experimental arthritis. Ovariectomized DBA/1 ... Treatment with estradiol potently inhibits experimental autoimmune arthritis. Interleukin-17-producing T helper cells (Th17) ... We report that E2 treatment results in an increase of Th17 cells in lymph nodes during the early phase of arthritis development ...
Experimental: Low Dose A low dose of LX3305; daily oral intake for 12 weeks ... Arthritis. Arthritis, Rheumatoid. Joint Diseases. Musculoskeletal Diseases. Rheumatic Diseases. Connective Tissue Diseases. ... Rheumatoid Arthritis Drug: LX3305 low dose Drug: LX3305 mid dose Drug: LX3305 high dose Drug: Placebo Phase 2 ... Study of LX3305 in Subjects With Active Rheumatoid Arthritis on Stable Methotrexate. The safety and scientific validity of this ...
Anti-rheumatoid arthritis mechanism of Sophorae Tonkinesis Radix et Rhizoma based on network pharmacology and experimental ... Artrite Experimental/tratamento farmacológico; Artrite Experimental/genética; Fator de Necrose Tumoral alfa; Interleucina-6; ... Texto completo: Disponível Coleções: Bases de dados internacionais Temas: Geral Assunto principal: Artrite Experimental / ... Texto completo: Disponível Coleções: Bases de dados internacionais Temas: Geral Assunto principal: Artrite Experimental / ...
... and stiffness of arthritis can limit your movement. Medicines can help manage your symptoms so that you can continue to lead an ... These treatments are quite new and mostly experimental. Please talk with your provider first before having them. ... But they do not seem to help the joint grow new cartilage or keep arthritis from getting worse. Some doctors recommend a trial ... The results have shown it to be safe and perhaps helpful for people with early arthritis. Talk to your provider before having ...
Automated Quantification of Experimental Arthritis based on In vivo PET/CT lmaging. Bianca Hoffmann. Cell and Molecular Biology ...
Arthritis, Experimental / physiopathology* * Bridged Bicyclo Compounds, Heterocyclic / pharmacology * Excitatory Amino Acid ... Group II mGluRs modulate baseline and arthritis pain-related synaptic transmission in the rat medial prefrontal cortex ... Here we evaluated their ability to restore pyramidal output in an arthritis pain model. Whole-cell patch-clamp recordings of ...
Retired Olympians Face Higher Odds for Arthritis Second Death in Trial of Experimental Alzheimers Drug Is Raising Concerns ...
Experimental: NI-071 Proposed biosimilar. Biological: NI-071 20 ml vial containing 100 mg for a final reconstitution volume of ... Arthritis. Arthritis, Rheumatoid. Joint Diseases. Musculoskeletal Diseases. Rheumatic Diseases. Connective Tissue Diseases. ... A Phase 3 Study of NI-071 in Patients With Rheumatoid Arthritis (RADIANCE). The safety and scientific validity of this study is ... Patients with a diagnosis of rheumatoid arthritis (RA) as defined by the 2010 ACR and European League Against Rheumatism (EULAR ...
An experimental model aggravated by foam macrophage infiltration. Overview of attention for article published in Arthritis ... Hypercholesterolemia boosts joint destruction in chronic arthritis. An experimental model aggravated by foam macrophage ... of this study was to determine whether hypercholesterolemia increases articular damage in a rabbit model of chronic arthritis. ... Hypercholesterolemia boosts joint destruction in chronic arthritis. ...
Experimental: Baricitinib Low Dose Baricitinib administered orally.. Drug: Baricitinib Administered orally.. Other Name: ... Arthritis. Arthritis, Rheumatoid. Joint Diseases. Musculoskeletal Diseases. Rheumatic Diseases. Connective Tissue Diseases. ... A Study of Baricitinib (LY3009104) in Participants With Rheumatoid Arthritis (RA-BRIDGE). The safety and scientific validity of ... A Randomized, Active-Controlled, Parallel-Group, Phase 3b/4 Study of Baricitinib in Patients With Rheumatoid Arthritis. ...
Our previous study showed that in experimental osteoporosis, chiropractic manipulation (CM) exerts protective effects on bone. ... An experimental model aggravated by foam macrophage infiltration. Arthritis Res. Ther.15, R81 (2013). ... Lugo, L. et al. Effects of PTH [1-34] on synoviopathy in an experimental model of osteoarthritis preceded by osteoporosis. ... Naredo, E. et al. Validation of musculoskeletal ultrasound in the assessment of experimental gout synovitis. Ultrasound. Med. ...
Arthritis Rheum. 2001, 44, 2539-2547. [Google Scholar] [CrossRef]. *Dieppe, P.A.; Lohmander, L.S. Pathogenesis and management ... In vivo Experimental Studies. This study was conducted at the Experimental Laboratory for the Study of Pain (LEED). All ... A-RX from the healthy rat paw (CLEAN); B1 and B2-RX from the rat paw with experimental model of MIA-induced osteoarthritis- ... A-RX from the healthy rat paw (CLEAN); B1 and B2-RX from the rat paw with experimental model of MIA-induced osteoarthritis- ...
Arthritis Rheum 1975. 18:361-368. View this article via: PubMed CrossRef Google Scholar ... mice are resistant to experimental BP; (c) NE-/- mice reconstituted with NE+/+ neutrophils become susceptible to experimental ... A critical role for neutrophil elastase in experimental bullous pemphigoid. Zhi Liu,1 Steven D. Shapiro,2,3 Xiaoye Zhou,1 Sally ... The role of complement in experimental bullous pemphigoid. J Clin Invest 1995. 95:1539-1544. View this article via: JCI PubMed ...
Septic arthritis of native hip joints is an uncommon condition in adults in Western Europe, but continues to present a ... 2022 Experimental Medicine Division, John Radcliffe Hospital, Headley Way, Headington, Oxford, OX3 9DU ... OBJECTIVES: Septic arthritis of native hip joints is an uncommon condition in adults in Western Europe, but continues to ... Native hip joint septic arthritis in 20 adults: delayed presentation beyond three weeks predicts need for excision arthroplasty ...
keywords = "Adjuvant-induced arthritis, Anti-arthritis, Herbal medicine, Pro-inflammatory cytokines, QFGJS, Rheumatoid ... on rat experimental adjuvant-induced arthritis, Biochemical and Biophysical Research Communications, vol. 337, no. 2, pp. 586- ... on rat experimental adjuvant-induced arthritis. / Cai, Xiong; Zhou, Hua; Yuen, Fan Wong; Xie, Ying; Zhong, Qiu Liu; Jiang, Zhi ... on rat experimental adjuvant-induced arthritis. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 337, No. 2 ...
Experimental Arthritis IDA. 61087. RGD. rheumatoid arthritis MODEL: induced. IAGP. XCO:0000263, XCO:0000280, XCO:0000281,XCO: ... Experimental Arthritis MODEL: induced. IAGP. XCO:0000265. 61087. compared to LEW.1AV1/Kini control. RGD. ... Experimental Arthritis MODEL: control. IAGP. XCO:0000265. 61087. compared to DA/ZtmKini. RGD. ... Only show annotations with direct experimental evidence (0 objects hidden). Term. Qualifier. Evidence. With. Reference. Notes. ...
Vascular endothelial growth factor in patients with psoriatic arthritis. Clinical and experimental rheumatology, 2007, 25:305-8 ... Previous experimental studies revealed that skin exposure to contact allergens induced a selective type 1 T-helper (Th1) cell ... Previous experimental studies revealed that skin exposure to contact allergens induced a selective Th1-type cytokine response ... Journal of experimental medicine, 1994, 180:1141-6.. *Fink AM et al. ...
... are the predominant sources of TNF-α in zymosan-induced arthritis. Cytokine. 1996;8:130-3. ... Estudo experimental para comparar a resposta inflamatória decorrente da distensão líquida ou gasosa em cavalos submetidos à ... An experimental study to compare inflammatory response due to liquid or gas joint distension in horses submitted to arthroscopy ... An experimental study to compare inflammatory response due to liquid or gas joint distension in horses submitted to arthroscopy ...
Lapachol, a compound targeting pyrimidine metabolism, ameliorates experimental autoimmune arthritis *Peres, RS, Santos, GB, ...
Experimental Drug Combination Curbs Chikungunya Arthritis in Mice. May 16, 2017. • By Bryn Nelson, PhD ... Rheumatology Case Report: Monoarticular Arthritis in Pregnancy. October 18, 2017. • By Aiza Tariq, MD, and Harry D. Fischer, MD ... Diagnosis, Treatment Updates for Lyme Arthritis. July 14, 2017. • By Lara C. Pullen, PhD ...
where σpeak and σeq are the peak and equilibrium stresses, respectively, which were defined by the experimental data; t is time ... 2). These data add a novel experimental phenomenon to an already complex picture of cartilage mechanics (Dean et al., 2006; ... Experimental verification of the roles of intrinsic matrix viscoelasticity and tension-compression nonlinearity in the biphasic ... A) Representative set of experimental data. Top: stress and temperature as a function of time; bottom: temperature versus ...
Roches arthritis drug failed a clinical trial for COVID, demonstrating the risks and rewards of repurposing drugs. ... Experimental vaccines rely on old technologies for similar coronaviruses. It isnt easy to balance expedience with ... Roche tried to see if its arthritis drug could fight coronavirus. It didnt work. BYSy Mukherjee ... The Swiss drug giant was attempting to see whether or not Actemra, an anti-inflammatory which treats rheumatoid arthritis, ...
  • 2019 American College of Rheumatology/Arthritis Foundation guideline for the management of osteoarthritis of the hand, hip, and knee. (medlineplus.gov)
  • She is lead for clinical trials in inflammatory arthritis and north-west London CLRN Rheumatology lead. (imperial.ac.uk)
  • She is also Lead for Undergraduate Year 5 Rheumatology education at Imperial and an Arthritis Research UK student mentor. (imperial.ac.uk)
  • Arthritis & rheumatology (Hoboken, N.J. (nih.gov)
  • With her interest in translational and experimental medicine, she is developing a biomarker/imaging and drug discovery programme in Psoriatic arthritis (Psa)and Psoriasis (Pso) with Professor Dorian Haskard. (imperial.ac.uk)
  • MADRID - In patients with psoriatic arthritis, the combined end point of skin clearance and reduced disease activity was better with ixekizumab ( Taltz , Eli Lilly), an interleukin (IL)-17 inhibitor, than with adalimumab ( Humira , AbbVie), a TNF inhibitor, early results from the SPIRIT-H2H ( NCT03151551 ) trial show. (medscape.com)
  • Of the 566 study participants with active psoriatic arthritis who were naïve to biologic disease-modifying antirheumatic drugs, 283 were randomized to ixekizumab and 283 to adalimumab. (medscape.com)
  • The trial "confirms our clinical experiences, but is a strong indication that we need better drugs for psoriatic arthritis to control joint progression," Dörner told Medscape Medical News . (medscape.com)
  • Cite this: Interleukin-17 Edges TNF Inhibitor in Psoriatic Arthritis - Medscape - Jun 20, 2019. (medscape.com)
  • New findings hint that experimental drugs currently under development for diseases like cancer and Alzheimer's may have potential to treat rheumatoid arthritis patients too. (time.com)
  • According to the study authors, the findings suggest that drugs currently under development for the treatment of other diseases like cancer and Alzheimer's may also help treat rheumatoid arthritis. (time.com)
  • Indeed, experimental Notch-inhibiting drugs are currently being tested for the treatment of cancer and Alzheimer's - two conditions that also involve inflammation. (time.com)
  • Bacteria involved in periodontitis have been linked with Alzheimer's disease, aspiration pneumonia, rheumatoid arthritis and other common disorders. (eurekalert.org)
  • Orlando, Fla. (April 7, 2019) - Researchers are reporting new findings on how bacteria involved in gum disease can travel throughout the body, exuding toxins connected with Alzheimer's disease, rheumatoid arthritis and aspiration pneumonia. (eurekalert.org)
  • People with genetic risk factors that make them susceptible to rheumatoid arthritis or Alzheimer's disease should be extremely concerned with preventing gum disease. (eurekalert.org)
  • An experimental drug that blocks gingipains, known as COR388, is currently in phase 1 clinical trials for Alzheimer's disease. (eurekalert.org)
  • Scientists from the universities of Southampton and Glasgow have uncovered a new method for culturing adult stem cells which could lead to the creation of revolutionary stem cell therapies for conditions such as arthritis, Alzheimer's disease and Parkinson's disease. (cellmedicinesociety.org)
  • Occasionally, medical professionals administer stem cell therapy to patients who have Alzheimer's disease and arthritis. (healthcarebusinesstoday.com)
  • Osteoarthritis is the most common type of arthritis, according to the National Health Service, USA. (edu.pk)
  • The most common form of arthritis is osteoarthritis (OA). (redolaughlin.com)
  • Unlike osteoarthritis, which is caused by wear and tear, rheumatoid arthritis is an auto-immune disease. (hippocraticpost.com)
  • Nonsteroidal anti-inflammatory drugs are commonly used in the treatment or management of rheumatoid arthritis, osteoarthritis, and ankylosing spondylitis and for its anti-inflammatory and analgesic effects. (vietketnoi.net)
  • This study demonstrated the multi-component, multi-target and multi-pathway synergistic effect of STRR in the treatment of RA, laying an experimental basis for clinical application of this medicine . (bvsalud.org)
  • To analyze the anti-arthritic effects of QFGJS (a pharmaceutical preparation from herbs) on rheumatoid arthritis, adjuvant-induced arthritis (AIA) was established in male SD rats, and two administration protocols, i.e., oral treatment with different doses of QFGJS on the day of arthritis induction or on the day when visible clinical signs of arthritis occurred, were initiated and continued until day 30. (edu.hk)
  • A 2016 review article of several small-scale clinical trials found evidence to support the use of frankincense and other Boswellian resin products for managing arthritis symptoms. (medicalnewstoday.com)
  • Dr. Abraham has successfully co-supervised MD and clinical PhD students in experimental translational inflammatory arthritis. (imperial.ac.uk)
  • Methods Findings in Experimental Clinical Pharmacology. (bvsalud.org)
  • Scopolamine, a muscarinic receptor antagonist is widely to induce cognitive / memory impairment in clinical research (human volunteers) and in experimental animals. (neurofit.com)
  • As the rheumatoid arthritis device uses a similar therapeutic stimulation strategy, this approval for use in clinical trials will accelerate its development. (hippocraticpost.com)
  • CONCLUSION: Intraosseous bone marrow inflammation was observed in carrageenan-induced inflammatory arthritis in rats and rabbits. (jrheum.org)
  • We have searched for disease-linked non-MHC loci in an F2 intercross between DA rats and MHC-identical but arthritis-resistant LEW.1AV1 rats. (mcw.edu)
  • Therefore, the present study was carried out to evaluate the antiarthritic activity of CS in rats in two experimental models. (who.int)
  • Methods: The antiarthritic activity of CS seed hydroalcoholic extract (CSHE) was evaluated in adult Wistar rats by using two experimental models, viz. (who.int)
  • Using gene editing, the team took rats and knocked out 14-3-3 zeta, preventing its production, in the expectation that the rats may have reduced inflammatory arthritis. (iflscience.com)
  • Instead, the rats developed severe early-onset arthritis, with the disease resulting in bone loss, immune cell infiltration, and destruction of joints. (iflscience.com)
  • However, when they supplemented different rats with a vaccine packed full of 14-3-3 zeta, prior to them developing the condition, it resulted in suppression of arthritis onset in all rat models. (iflscience.com)
  • Anti-rheumatoid arthritis mechanism of Sophorae Tonkinesis Radix et Rhizoma based on network pharmacology and experimental verification]. (bvsalud.org)
  • Based on the network pharmacology , molecular docking , and animal experiment , this study explored the anti- rheumatoid arthritis (RA) mechanism of Sophorae Tonkinesis Radix et Rhizoma(STRR). (bvsalud.org)
  • An experimental study to compare inflammatory response due to liquid or gas joint distension in horses submitted to arthroscopy 1 1 Research performed at Laboratory of Pharmacology, Department of Pathology and Animal Hospital, Faculty of Veterinary Medicine, University of Sao Paulo (USP), Brazil. (scielo.br)
  • This new treatment will help the large number of people with rheumatoid arthritis who do not respond to drug therapy and suffer ongoing pain and disability. (hippocraticpost.com)
  • As the stimulation kickstarts the body's natural mechanisms, people with rheumatoid arthritis will not experience the side effects associated with current drug therapies. (hippocraticpost.com)
  • To provide a frame to estimate the systemic impact (side/adverse events) of (novel) therapeutic targets by taking into consideration drugs potential on the numerous districts involved in rheumatoid arthritis (RA) from the inflammatory and immune response to the gut-intestinal (GI) microbiome. (frontiersin.org)
  • Ovariectomized DBA/1 mice treated with 17β-estradiol (E2) or placebo were subjected to collagen-induced arthritis (CIA), and arthritis development was assessed. (biomedcentral.com)
  • Combined collagen-induced arthritis and organic dust-induced airway inflammation to model inflammatory lung disease in rheumatoid arthritis. (cdc.gov)
  • The collagen-induced arthritis (CIA) model was combined with the organic dust extract (ODE) airway inflammatory model to assess bone/joint-lung inflammatory outcomes. (cdc.gov)
  • We present a case of tuberculous peritonitis in a 46-year-old woman with rheumatoid arthritis treated with adalimumab, and we review the association between anti-tumour necrosis factor therapy and tuberculosis. (scirp.org)
  • Herein, we present a case of tuberculous peritonitis in a patient with rheumatoid arthritis treated with adalimumab. (scirp.org)
  • Sex differences in remission rates over 24 weeks among three different biological treatments compared to conventional therapy in patients with early rheumatoid arthritis (NORD-STAR): a post-hoc analysis of a randomised controlled trial. (uio.no)
  • The team, from Eindhoven University of Technology in the Netherlands, believes this new method will lead to the development of smart drugs that more effectively treat autoimmune diseases like rheumatoid arthritis and Crohn's disease. (worldhealth.net)
  • Researchers are working on ways to use the technology in the fight against chronic diseases like rheumatoid arthritis and Crohn's Disease. (worldhealth.net)
  • 2-5 Scientists have been familiar with this enzyme for some time due to its role in occurrence of arthritis, asthma and Crohn's disease, which is why the pharmaceutical industry aims to develop a safe drug to alleviate the effect of 5-lipoxygenase. (our-arthritis.com)
  • Frankincense extracts have proved effective in patients suffering from rheumatoid arthritis, Crohn's disease, ulcerative colitis and asthma. (our-arthritis.com)
  • Reducing inflammation in rheumatoid arthritis research from the Bionics Institute in Melbourne, Australia: Our researchers are developing a drug-free treatment to treat rheumatoid arthritis using vagus nerve stimulation . (hippocraticpost.com)
  • Despite advances in pharmacological drug therapies, 40% of rheumatoid arthritis patients fail to respond to first order therapy, and 30% of those fail second order therapy (Bystrom et al. (hippocraticpost.com)
  • Potempa will present the research at the American Association of Anatomists annual meeting during the 2019 Experimental Biology meeting , held April 6-9 in Orlando, Fla. (eurekalert.org)
  • The purpose of the study is to evaluate the safety, tolerability, and effectiveness of LX3305 versus a placebo control in subjects with active rheumatoid arthritis on stable methotrexate therapy. (clinicaltrials.gov)
  • Inflammation and biologic therapy in patients with rheumatoid arthritis achieving versus not achieving ACR/EULAR Boolean remission in a treat-to-target study. (uio.no)
  • Arthritis Research and Therapy, 16 (3). (whiterose.ac.uk)
  • This therapy can help ease joint pain, restore balance, and address various kinds of arthritis. (sport-topics.com)
  • They may also provide clues to the etiology of inflammatory diseases in humans, including rheumatoid arthritis. (mcw.edu)
  • There are currently other gamma-secretase inhibitors in phase II trials for various cancers, but there have been no trials of Notch inhibitors on inflammatory diseases like rheumatoid arthritis, according to Hu. (time.com)
  • Do bacterial antigens persist in reactive arthritis? (utu.fi)
  • In addition to the drunken driving charge, he claims to suffer from reactive arthritis, anorexia, and anxiety. (funzalo.com)
  • Articular damage is associated with intraosseous inflammation in the subchondral bone marrow of joints affected by experimental inflammatory arthritis and is modified by zoledronate treatment. (jrheum.org)
  • E2-treated mice with established CIA showed reduced severity of arthritis and fewer Th17 cells in joints compared with controls. (biomedcentral.com)
  • We report that E2 treatment results in an increase of Th17 cells in lymph nodes during the early phase of arthritis development, but leads to a decrease of Th17 in joints during established arthritis. (biomedcentral.com)
  • OBJECTIVES: Septic arthritis of native hip joints is an uncommon condition in adults in Western Europe, but continues to present a challenge to medical and surgical management. (ox.ac.uk)
  • Millions of Americans suffer from rheumatoid arthritis, an autoimmune disease that leads the immune system to essentially attacks its own joints, causing inflammation and pain. (time.com)
  • When macrophage development goes wrong, however, the cells can cause widespread inflammation and destruction - attacking joints and contributing to rheumatoid arthritis. (time.com)
  • Consequently, in arthritic mice lacking autophagy in LECs, pathogenic Th17 cell migration toward LEC-derived S1P gradients and egress from LNs are enhanced, as well as infiltration of inflamed joints, resulting in exacerbated arthritis. (bvsalud.org)
  • Rheumatoid arthritis is an autoimmune disorder in which immune cells attack the lining of joints, degrading them progressively over time. (iflscience.com)
  • There is no cure for rheumatoid arthritis, but early diagnosis can help alleviate symptoms to a liveable degree through the use of disease-modifying anti-rheumatic drugs (DMARDs), which block the effects of chemicals released when the immune system attacks the joints. (iflscience.com)
  • This study contributes to the understanding of the role of estrogen in the development of autoimmune arthritis and opens up new fields for research concerning the sex bias in autoimmune disease. (biomedcentral.com)
  • The researchers also report evidence on the bacterium's role in the autoimmune disease rheumatoid arthritis, as well as aspiration pneumonia, a lung infection caused by inhaling food or saliva. (eurekalert.org)
  • The interaction of inhalation-induced airway inflammation and arthritis induction resulted in compartmentalized responses with the greatest degree of arthritis and bone loss in male mice with combined exposures. (cdc.gov)
  • The use of ivy leaf functional food ingredient extract for the treatment of inflammation and arthritis has opened up a safe, effective and cost-effective herbal treatment for these chronic diseases. (vietketnoi.net)
  • The purpose of this study is to demonstrate similarity of NI-071 (proposed biosimilar to infliximab) to US REMICADE® (reference product) in terms of safety and efficacy in patients with rheumatoid arthritis (RA) not adequately responding to methotrexate (MTX). (clinicaltrials.gov)
  • Synergistic effect of antiangiogenic nanotherapy combined with methotrexate in the treatment of experimental inflammatory arthritis. (semanticscholar.org)
  • To compare the modifications in lipids between patients with rheumatoid arthritis (RA) receiving etanercept plus methotrexate (ETA + MTX) versus methotrexate (MTX) and their relationship with serum levels of tumor necrosis factor-alpha (TNF-α). (hindawi.com)
  • 7 Indeed, mice overexpressing human TNFα (hTNFα tg) show increased osteoclastogenesis and destructive arthritis closely resembling RA. (bmj.com)
  • Lymph node Th17 cells were also examined in ovariectomized estrogen receptor α-knockout mice (ERα −/− ) and wild-type littermates, treated with E2 or placebo and subjected to antigen-induced arthritis. (biomedcentral.com)
  • Over-the-counter pain relievers can help with your arthritis symptoms. (medlineplus.gov)
  • The Swiss drug giant was attempting to see whether or not Actemra, an anti-inflammatory which treats rheumatoid arthritis, could be promising for patients who had been hospitalized for COVID-19 with severe symptoms. (fortune.com)
  • Arthritis is a general term that describes a family of medical conditions that have the symptoms of joint inflammation, pain, and stiffness. (medicalnewstoday.com)
  • As well as natural remedies, medical treatments, regular exercise, and proper nutrition can also help people manage their arthritis symptoms. (medicalnewstoday.com)
  • According to 2017 research , borage oil may help improve symptoms of rheumatoid arthritis (RA), although it can also cause some negative side effects. (medicalnewstoday.com)
  • According to the Arthritis Foundation , the active compounds in frankincense possess anti-inflammatory properties that could help relieve arthritis symptoms. (medicalnewstoday.com)
  • To compound this issue, many patients with rheumatoid arthritis don't respond to treatment and continue to live with debilitating symptoms. (hippocraticpost.com)
  • The marked reduction of inflammatory symptoms by ivy leaf extract shows the potential of this medicinal herb with anti-inflammatory, arthritis, rheumatism, gout. (vietketnoi.net)
  • Arthritis was induced in New Zealand white rabbits in 5 treatment groups: normal, arthritis, zoledronate-treated from arthritis induction, or 2 or 4 weeks after induction. (jrheum.org)
  • Treatment with estradiol potently inhibits experimental autoimmune arthritis. (biomedcentral.com)
  • Hence, 3 experimental treatment groups (control, 0.5 mg/L of BV, and 1 mg/L of BV) were allocated to 3 replicates of 5,000 one-day-old chicks each. (blogspot.com)
  • Background & objectives: Coriandrum sativum (CS), has been widely used in traditional systems of medicine for treatment of rheumatoid arthritis. (who.int)
  • Researchers at the Bionics Institute are developing an innovative drug-free treatment for rheumatoid arthritis. (hippocraticpost.com)
  • Why is a new treatment for rheumatoid arthritis needed? (hippocraticpost.com)
  • As such, if abdominal stimulation proves an effective treatment of experimental arthritis, the treatment will be translated into the clinic quickly. (hippocraticpost.com)
  • The treatment of particular bone fractures (i.e., wrist and arm), rheumatoid arthritis or post-surgical orthopedic rehabilitation require the prolonged immobilization of skeletal muscle system. (unina.it)
  • Rheumatoid arthritis (RA) is a chronic inflammatory disease that results in severe destruction of articular cartilage and bone. (bmj.com)
  • All animals homozygous for DA alleles at both loci developed severe arthritis, whereas all those homozygous for LEW.1AV1 alleles were resistant. (mcw.edu)
  • Native hip joint septic arthritis in 20 adults: delayed presentation beyond three weeks predicts need for excision arthroplasty. (ox.ac.uk)
  • history of septic arthritis, 3.4. (who.int)
  • Additionally, she is keen to identify molecular effects of biologics in inflammatory arthritides including Psa, ankylosing spondylitis and rheumatoid arthritis with an aim to understanding the biology of these diseases in relation to phenotype and disease activity. (imperial.ac.uk)
  • Alterations in skin microvascular function in patients with rheumatoid arthritis and ankylosing spondylitis. (krakow.pl)
  • We conducted a prospective, multicenter, quasi-experimental, single-blinded study at 2 VHA medical centers to assess health services use, cost, and satisfaction during 12 months among 2 groups of homeless veterans: 1) veterans receiving VHA homeless-tailored primary care (Homeless-Patient Aligned Care Team [H-PACT]) and 2) veterans receiving traditional primary care services (PACT). (cdc.gov)
  • The aim of this study was to investigate the effects of estrogen on Th17 cells in experimental arthritis. (biomedcentral.com)
  • This is the first study in which the effects of E2 on Th17 cells have been characterized in experimental autoimmune arthritis. (biomedcentral.com)
  • The aim of this study was to determine whether hypercholesterolemia increases articular damage in a rabbit model of chronic arthritis. (altmetric.com)
  • This post-marketing study is designed to compare the safety of baricitinib versus tumor necrosis factor (TNF) inhibitors with respect to venous thromboembolic events (VTEs) when given to participants with rheumatoid arthritis. (clinicaltrials.gov)
  • Our previous study showed that in experimental osteoporosis, chiropractic manipulation (CM) exerts protective effects on bone. (nature.com)
  • Krystal is the Project Coordinator for the Phone-Based Walk with Ease Program in Adults with Arthritis Study. (sc.edu)
  • The authors of the current study suggest these compounds might prove helpful for rheumatoid arthritis as well. (time.com)
  • DNA computing is a largely experimental field of scientific study. (worldhealth.net)
  • Randomized Control Trial (Experimental Study). (edu.pk)
  • Treatments with QFGJS using both administration protocols significantly suppressed the incidence and severity of arthritis in a dose-dependent manner, showing dramatic reduction of paw swelling and ESR throughout the disease progression of AIA. (edu.hk)
  • Rheumatoid arthritis (RA) is a multifaceted autoimmune, chronic and inflammatory disease with, to date, unclear etiology. (frontiersin.org)
  • Rheumatoid arthritis (RA) is characterized by extra-articular involvement including lung disease, yet the mechanisms linking the two conditions are poorly understood. (cdc.gov)
  • Data also support suppression of the lung inflammatory response, but increases in extracellular matrix protein deposition/interstitial disease in the setting of arthritis. (cdc.gov)
  • Cardiovascular disease constitutes the main cause of death for patients with long disease duration in rheumatoid arthritis (RA) [ 1 ]. (hindawi.com)
  • IMSEAR at SEARO: Evaluation of disease modifying activity of Coriandrum sativum in experimental models. (who.int)
  • Nair Vinod, Singh Surender, Gupta Y K. Evaluation of disease modifying activity of Coriandrum sativum in experimental models. (who.int)
  • 8 Hundreds of published scientific studies have established a link between leukotrienes and the occurrence of cardiovascular disease9, carcinoma 10-16, arthritis 17-19 , and respiratory diseases, such as asthma and chronic obstructive pulmonary disease (COPD) 8 . (our-arthritis.com)
  • PURPOSE: To assess comparatively the inflammatory response that follows CO2 or Ringer's lactate joint capsular distension of horses submitted to experimental arthroscopy METHODS: Each animal was submitted to a bilateral tarsocrural arthroscopy employing gas distention in one joint and fluid distention in the contralateral joint. (scielo.br)
  • Optimal care of patients with rheumatoid arthritis (RA) consists of an integrated approach that includes both pharmacologic and nonpharmacologic therapies. (medscape.com)
  • The Norfolk Arthritis Register (NOAR) has been recruiting and following patients with early inflammatory polyarthritis (IP) since 1989. (ox.ac.uk)
  • Approximately three-quarters of the patients followed satisfy classification criteria for rheumatoid arthritis (RA) by 5 years from symptom onset. (ox.ac.uk)
  • To determine the effects of manual traction in knee arthritis patients in comparison to electrotherapy (TENS). (edu.pk)
  • Arthritis Care and Research, 69 (11). (whiterose.ac.uk)
  • Hu says the next step in the research will be to measure the involvement of the Notch pathway in animal models of rheumatoid arthritis and to evaluate the efficacy of Notch inhibitors in treating arthritis in animals. (time.com)
  • An experimental protein-based vaccine against rheumatoid arthritis has shown huge promise in early animal models, according to new research from the University of Toledo. (iflscience.com)
  • This mechanism was previously unrecognized in rheumatoid arthritis research and may have huge implications if it translates to humans. (iflscience.com)
  • His research spanned most of the twentieth century, a time when the field of embryology evolved from being a purely descriptive pursuit to one of experimental research, to that of incorporating molecular biology into the research lab. (asu.edu)
  • The single-stranded RNA molecule that has negative present review discusses the findings of experimental polarity for measles and mumps and positive polarity work carried out at the National Institute for Biological for rubella. (who.int)
  • Evaluation of endothelial function and skin microcirculation in the early phase of chronic inflammation in inflammatory arthritis. (krakow.pl)
  • In lab studies, Hu and her colleagues tested an experimental Notch inhibitor called GSI-34 and found that the drug inhibited macrophage function. (time.com)
  • Serum autoantibody and inflammatory marker concentrations varied among experimental groups. (cdc.gov)
  • Astragalus is also specifically use to treat fibrotic changes in the tissues, as can occur with numerous autoimmune diseases, including diabetes, nephritis, hepatitis , arthritis, and dermatitis. (ndnr.com)
  • It is claimed to have an immune-stimulating effect that helps fight cancer, AIDS, arthritis, diabetes, and hepatitis B & C. (asapland.com)
  • Here, we investigated the role of autophagy during joint destruction in arthritis. (bmj.com)
  • Hypercholesterolemia boosts joint destruction in chronic arthritis. (altmetric.com)
  • OBJECTIVE: We studied histological changes in the cell populations of subchondral bone marrow resulting from inflammation in animal models of arthritis. (jrheum.org)
  • In general, macrophages are thought to play a pathogenic role in rheumatoid arthritis by secreting inflammatory mediators that contribute to joint inflammation and bone erosion. (time.com)
  • Identification of rat susceptibility loci for adjuvant-oil-induced arthritis. (mcw.edu)
  • Special Theme ± Immunization Safety seed material for its virological,serological,and acute arthritis in some adult women (12). (who.int)
  • Experimental vaccines rely on old technologies for similar coronaviruses. (fortune.com)
  • Propolis has plenty of biological and pharmacological properties and its mechanisms of action have been widely investigated in the last years, using different experimental models in vitro and in vivo. (blogspot.com)
  • According to the Arthritis Foundation , the condition affects over 50 million adults and 300,000 children in the United States. (medicalnewstoday.com)
  • In arthritis, these cell types and tissues displayed a shift of 1-2 grades higher (toward inflammation). (jrheum.org)
  • Results Autophagy was activated in osteoclasts of human rheumatoid arthritis (RA) showing increased expression of Beclin1 and Atg7. (bmj.com)
  • We investigated whether autophagy in LECs modulates T cell activation in experimental arthritis. (bvsalud.org)
  • Results: CSHE produced a dose dependent inhibition of joint swelling as compared to control animals in both, formaldehyde and CFA induced arthritis. (who.int)