Arsenites: Inorganic salts or organic esters of arsenious acid.Sodium Compounds: Inorganic compounds that contain sodium as an integral part of the molecule.Arsenite Transporting ATPases: Efflux pumps that use the energy of ATP hydrolysis to pump arsenite across a membrane. They are primarily found in prokaryotic organisms, where they play a role in protection against excess intracellular levels of arsenite ions.Arsenates: Inorganic or organic salts and esters of arsenic acid.Arsenic: A shiny gray element with atomic symbol As, atomic number 33, and atomic weight 75. It occurs throughout the universe, mostly in the form of metallic arsenides. Most forms are toxic. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), arsenic and certain arsenic compounds have been listed as known carcinogens. (From Merck Index, 11th ed)Ion Pumps: A general class of integral membrane proteins that transport ions across a membrane against an electrochemical gradient.Antimony: A metallic element that has the atomic symbol Sb, atomic number 51, and atomic weight 121.75. It is used as a metal alloy and as medicinal and poisonous salts. It is toxic and an irritant to the skin and the mucous membranes.Teratogens: An agent that causes the production of physical defects in the developing embryo.Arsenate Reductases: Oxidoreductases that specifically reduce arsenate ion to arsenite ion. Reduction of arsenate is a critical step for its biotransformation into a form that can be transported by ARSENITE TRANSPORTING ATPASES or complexed by specific sulfhydryl-containing proteins for the purpose of detoxification (METABOLIC DETOXIFICATION, DRUG). Arsenate reductases require reducing equivalents such as GLUTAREDOXIN or AZURIN.Arsenicals: Inorganic or organic compounds that contain arsenic.Aquaglyceroporins: A subgroup of aquaporins that transport WATER; GLYCEROL; and other small solutes across CELL MEMBRANES.Antimony Potassium Tartrate: A schistosomicide possibly useful against other parasites. It has irritant emetic properties and may cause lethal cardiac toxicity among other adverse effects.Alcaligenes faecalis: The type species of gram negative bacteria in the genus ALCALIGENES, found in soil. It is non-pathogenic, non-pigmented, and used for the production of amino acids.Ectothiorhodospiraceae: A family in the order Chromatiales, class GAMMAPROTEOBACTERIA. These are haloalkaliphilic, phototrophic bacteria that deposit elemental sulfur outside their cells.Pteris: A plant genus of the family PTERIDACEAE. Members contain entkaurane DITERPENES. The name is similar to bracken fern (PTERIDIUM).Arsenic Poisoning: Disorders associated with acute or chronic exposure to compounds containing ARSENIC (ARSENICALS) which may be fatal. Acute oral ingestion is associated with gastrointestinal symptoms and an encephalopathy which may manifest as SEIZURES, mental status changes, and COMA. Chronic exposure is associated with mucosal irritation, desquamating rash, myalgias, peripheral neuropathy, and white transverse (Mees) lines in the fingernails. (Adams et al., Principles of Neurology, 6th ed, p1212)Cadmium Chloride: A cadmium halide in the form of colorless crystals, soluble in water, methanol, and ethanol. It is used in photography, in dyeing, and calico printing, and as a solution to precipitate sulfides. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Ralstonia: A genus in the family BURKHOLDERIACEAE, comprised of many species. They are associated with a variety of infections including MENINGITIS; PERITONITIS; and URINARY TRACT INFECTIONS.Oxalobacteraceae: A family of gram-negative bacteria in the class BETAPROTEOBACTERIA. There are at least eight genera.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Respiratory Mucosa: The mucous membrane lining the RESPIRATORY TRACT, including the NASAL CAVITY; the LARYNX; the TRACHEA; and the BRONCHI tree. The respiratory mucosa consists of various types of epithelial cells ranging from ciliated columnar to simple squamous, mucous GOBLET CELLS, and glands containing both mucous and serous cells.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Leishmania donovani: A parasitic hemoflagellate of the subgenus Leishmania leishmania that infects man and animals and causes visceral leishmaniasis (LEISHMANIASIS, VISCERAL). The sandfly genera Phlebotomus and Lutzomyia are the vectors.Antiprotozoal Agents: Substances that are destructive to protozoans.Leishmaniasis, Visceral: A chronic disease caused by LEISHMANIA DONOVANI and transmitted by the bite of several sandflies of the genera Phlebotomus and Lutzomyia. It is commonly characterized by fever, chills, vomiting, anemia, hepatosplenomegaly, leukopenia, hypergammaglobulinemia, emaciation, and an earth-gray color of the skin. The disease is classified into three main types according to geographic distribution: Indian, Mediterranean (or infantile), and African.Leishmania: A genus of flagellate protozoa comprising several species that are pathogenic for humans. Organisms of this genus have an amastigote and a promastigote stage in their life cycles. As a result of enzymatic studies this single genus has been divided into two subgenera: Leishmania leishmania and Leishmania viannia. Species within the Leishmania leishmania subgenus include: L. aethiopica, L. arabica, L. donovani, L. enrietti, L. gerbilli, L. hertigi, L. infantum, L. major, L. mexicana, and L. tropica. The following species are those that compose the Leishmania viannia subgenus: L. braziliensis, L. guyanensis, L. lainsoni, L. naiffi, and L. shawi.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Phenalenes: A group of AROMATIC HYDROCARBONS that have three rings joined as a triad around a single carbon atom so all three are conjoined, in contrast to a linear arrangement (ANTHRACENES) or angular arrangement (PHENANTHRENES).Aspartate-Ammonia Ligase: An enzyme that catalyzes the formation of asparagine from ammonia and aspartic acid, in the presence of ATP. EC 6.3.1.1.ATP-Binding Cassette Transporters: A family of MEMBRANE TRANSPORT PROTEINS that require ATP hydrolysis for the transport of substrates across membranes. The protein family derives its name from the ATP-binding domain found on the protein.Atlases as Topic: Collections of illustrative plates, charts, etc., usually with explanatory captions.Urothelium: The epithelial lining of the URINARY TRACT.Gefarnate: A water insoluble terpene fatty acid used in the treatment of gastrointestinal ulcers; it facilitates the healing and function of mucosal tissue.DNA Methylation: Addition of methyl groups to DNA. DNA methyltransferases (DNA methylases) perform this reaction using S-ADENOSYLMETHIONINE as the methyl group donor.Urinary Bladder Neoplasms: Tumors or cancer of the URINARY BLADDER.Urinary Bladder: A musculomembranous sac along the URINARY TRACT. URINE flows from the KIDNEYS into the bladder via the ureters (URETER), and is held there until URINATION.Poly(ADP-ribose) Polymerases: Enzymes that catalyze the transfer of multiple ADP-RIBOSE groups from nicotinamide-adenine dinucleotide (NAD) onto protein targets, thus building up a linear or branched homopolymer of repeating ADP-ribose units i.e., POLY ADENOSINE DIPHOSPHATE RIBOSE.Linitis Plastica: A condition where the stomach wall becomes thickened, rubbery and loses its ability to distend. The stomach assumes a "leather bottle" shape. It is most often seen in adenocarcinoma of the stomach. The term is often used synonymously with diffuse adenocarcinoma of the stomach.Factor XIa: Activated form of factor XI. In the intrinsic pathway, Factor XI is activated to XIa by factor XIIa in the presence of cofactor HMWK; (HIGH MOLECULAR WEIGHT KININOGEN). Factor XIa then activates factor IX to factor IXa in the presence of calcium.Abstracting and Indexing as Topic: Activities performed to identify concepts and aspects of published information and research reports.Data Mining: Use of sophisticated analysis tools to sort through, organize, examine, and combine large sets of information.Mutagenicity Tests: Tests of chemical substances and physical agents for mutagenic potential. They include microbial, insect, mammalian cell, and whole animal tests.Oxides: Binary compounds of oxygen containing the anion O(2-). The anion combines with metals to form alkaline oxides and non-metals to form acidic oxides.Specific Gravity: The ratio of the density of a material to the density of some standard material, such as water or air, at a specified temperature.Geological Processes: Events and activities of the Earth and its structures.Epidermis: The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).Proto-Oncogene Proteins c-akt: A protein-serine-threonine kinase that is activated by PHOSPHORYLATION in response to GROWTH FACTORS or INSULIN. It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION. Three isoforms have been described in mammalian cells.Hypoxia-Inducible Factor 1, alpha Subunit: Hypoxia-inducible factor 1, alpha subunit is a basic helix-loop-helix transcription factor that is regulated by OXYGEN availability and is targeted for degradation by VHL TUMOR SUPPRESSOR PROTEIN.Hypoxia-Inducible Factor 1: A basic helix-loop-helix transcription factor that plays a role in APOPTOSIS. It is composed of two subunits: ARYL HYDROCARBON RECEPTOR NUCLEAR TRANSLOCATOR and HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT.DNA Polymerase beta: A DNA repair enzyme that catalyzes DNA synthesis during base excision DNA repair. EC 2.7.7.7.DNA Repair: The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.DNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.DNA-(Apurinic or Apyrimidinic Site) Lyase: A DNA repair enzyme that catalyses the excision of ribose residues at apurinic and apyrimidinic DNA sites that can result from the action of DNA GLYCOSYLASES. The enzyme catalyzes a beta-elimination reaction in which the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. This enzyme was previously listed under EC 3.1.25.2.DNA Glycosylases: A family of DNA repair enzymes that recognize damaged nucleotide bases and remove them by hydrolyzing the N-glycosidic bond that attaches them to the sugar backbone of the DNA molecule. The process called BASE EXCISION REPAIR can be completed by a DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE which excises the remaining RIBOSE sugar from the DNA.

Overexpression of the multidrug resistance-associated protein (MRP1) in human heavy metal-selected tumor cells. (1/791)

Cellular and molecular mechanisms involved in the resistance to cytotoxic heavy metals remain largely to be characterized in mammalian cells. To this end, we have analyzed a metal-resistant variant of the human lung cancer GLC4 cell line that we have selected by a step-wise procedure in potassium antimony tartrate. Antimony-selected cells, termed GLC4/Sb30 cells, poorly accumulated antimony through an enhanced cellular efflux of metal, thus suggesting up-regulation of a membrane export system in these cells. Indeed, GLC4/Sb30 cells were found to display a functional overexpression of the multidrug resistance-associated protein MRP1, a drug export pump, as demonstrated by Western blotting, reverse transcriptase-polymerase chain reaction and calcein accumulation assays. Moreover, MK571, a potent inhibitor of MRP1 activity, was found to markedly down-modulate resistance of GLC4/Sb30 cells to antimony and to decrease cellular export of the metal. Taken together, our data support the conclusion that overexpression of functional MRP1 likely represents one major mechanism by which human cells can escape the cytotoxic effects of heavy metals.  (+info)

Expression cloning for arsenite-resistance resulted in isolation of tumor-suppressor fau cDNA: possible involvement of the ubiquitin system in arsenic carcinogenesis. (2/791)

Arsenic is a human carcinogen whose mechanism of action is unknown. Previously, this laboratory demonstrated that arsenite acts as a comutagen by interfering with DNA repair, although a specific DNA repair enzyme sensitive to arsenite has not been identified. A number of stable arsenite-sensitive and arsenite-resistant sublines of Chinese hamster V79 cells have now been isolated. In order to gain understanding of possible targets for arsenite's action, one arsenite-resistant subline, As/R28A, was chosen as a donor for a cDNA expression library. The library from arsenite-induced As/R28A cells was transfected into arsenite-sensitive As/S5 cells, and transfectants were selected for arsenite-resistance. Two cDNAs, asr1 and asr2, which confer arsenite resistance to arsenite-hypersensitive As/S5 cells as well as to wild-type cells, were isolated. asr1 shows almost complete homology with the rat fau gene, a tumor suppressor gene which contains a ubiquitin-like region fused to S30 ribosomal protein. Arsenite was previously shown to inhibit ubiquitin-dependent proteolysis. These results suggest that the tumor suppressor fau gene product or some other aspect of the ubiquitin system may be a target for arsenic toxicity and that disruption of the ubiquitin system may contribute to the genotoxicity and carcinogenicity of arsenite.  (+info)

Protein-damaging stresses activate c-Jun N-terminal kinase via inhibition of its dephosphorylation: a novel pathway controlled by HSP72. (3/791)

Various stresses activate the c-Jun N-terminal kinase (JNK), which is involved in the regulation of many aspects of cellular physiology, including apoptosis. Here we demonstrate that in contrast to UV irradiation, heat shock causes little or no stimulation of the JNK-activating kinase SEK1, while knocking out the SEK1 gene completely blocks heat-induced JNK activation. Therefore, we tested whether heat shock activates JNK via inhibition of JNK dephosphorylation. The rate of JNK dephosphorylation in unstimulated cells was high, and exposure to UV irradiation, osmotic shock, interleukin-1, or anisomycin did not affect this process. Conversely, exposure of cells to heat shock and other protein-damaging conditions, including ethanol, arsenite, and oxidative stress, strongly reduced the rate of JNK dephosphorylation. Under these conditions, we did not observe any effects on dephosphorylation of the homologous p38 kinase, suggesting that suppression of dephosphorylation is specific to JNK. Together, these data indicate that activation of JNK by protein-damaging treatments is mediated primarily by inhibition of a JNK phosphatase(s). Elevation of cellular levels of the major heat shock protein Hsp72 inhibited a repression of JNK dephosphorylation by these stressful treatments, which explains recent reports of the suppression of JNK activation by Hsp72.  (+info)

Complexes containing activating transcription factor (ATF)/cAMP-responsive-element-binding protein (CREB) interact with the CCAAT/enhancer-binding protein (C/EBP)-ATF composite site to regulate Gadd153 expression during the stress response. (4/791)

Gadd153, also known as chop, encodes a member of the CCAAT/enhancer-binding protein (C/EBP) transcription factor family and is transcriptionally activated by cellular stress signals. We recently demonstrated that arsenite treatment of rat pheochromocytoma PC12 cells results in the biphasic induction of Gadd153 mRNA expression, controlled in part through binding of C/EBPbeta and two uncharacterized protein complexes to the C/EBP-ATF (activating transcription factor) composite site in the Gadd153 promoter. In this report, we identified components of these additional complexes as two ATF/CREB (cAMP-responsive-element-binding protein) transcription factors having differential binding activities dependent upon the time of arsenite exposure. During arsenite treatment of PC12 cells, we observed enhanced binding of ATF4 to the C/EBP-ATF site at 2 h as Gadd153 mRNA levels increased, and enhanced binding of ATF3 complexes at 6 h as Gadd153 expression declined. We further demonstrated that ATF4 activates, while ATF3 represses, Gadd153 promoter activity through the C/EBP-ATF site. ATF3 also repressed ATF4-mediated transactivation and arsenite-induced activation of the Gadd153 promoter. Our results suggest that numerous members of the ATF/CREB family are involved in the cellular stress response, and that regulation of stress-induced biphasic Gadd153 expression in PC12 cells involves the ordered, sequential binding of multiple transcription factor complexes to the C/EBP-ATF composite site.  (+info)

Teratogen-induced cell death in postimplantation mouse embryos: differential tissue sensitivity and hallmarks of apoptosis. (5/791)

Teratogen-induced cell death is a common event in the pathogenesis associated with tissues destined to be malformed. Although the importance of this cell death is recognized, little information is available concerning the biochemistry of teratogen-induced cell death. We show that three teratogens, hyperthermia, cyclophosphamide and sodium arsenite induce an increase in cell death in day 9.0 mouse embryos with concurrent induction of DNA fragmentation, activation of caspase-3 and the cleavage of poly (ADP-ribose) polymerase (PARP). Teratogen-induced cell death is also selective, i. e., some cells within a tissue die while others survive. In addition, cells within some tissues die when exposed to teratogens while cells in other tissues are relatively resistant to teratogen-induced cell death. An example of the latter selectivity is seen in the cells of the developing heart, which are resistant to the cytotoxic potential of many teratogens. We show that the absence of cell death in the heart is accompanied by the complete lack of DNA fragmentation, activtion of caspase-3 and the cleavage of PARP.  (+info)

Pathways of As(III) detoxification in Saccharomyces cerevisiae. (6/791)

Saccharomyces cerevisiae has two independent transport systems for the removal of arsenite from the cytosol. Acr3p is a plasma membrane transporter that confers resistance to arsenite, presumably by arsenite extrusion from the cells. Ycf1p, a member of the ABC transporter superfamily, catalyzes the ATP-driven uptake of As(III) into the vacuole, also producing resistance to arsenite. Vacuolar accumulation requires a reductant such as glutathione, suggesting that the substrate is the glutathione conjugate, As(GS)3. Disruption of either the ACR3 or YCF1 gene results in sensitivity to arsenite and disruption of both genes produces additive hypersensitivity. Thus, Acr3p and Ycf1p represent separate pathways for the detoxification of arsenite in yeast.  (+info)

Sodium arsenite enhances copper accumulation in human lung adenocarcinoma cells. (7/791)

In this report, we found that arsenite-resistant human lung adenocarcinoma cells, CL3R15, were more susceptible to CuCl2 than the parental CL3 cells. With the aid of atomic absorption spectrophotometry, we observed that CL3R15 cells accumulated more copper than CL3 cells. We further demonstrated that sodium arsenite treatment resulted in a dose-dependent increase of copper accumulation in the parental CL3 cells. In contrast, copper did not alter the levels of intracellular arsenite in CL3 cells treated in combination with sodium arsenite and CuCl2. Pretreatment of CL3 cells with sodium arsenite resulted in a significant increase of copper accumulation and cytotoxicity. These results indicate that intracellular copper accumulation is enhanced by arsenite. However, arsenite-enhanced copper accumulation was not observed in two fibroblastic cells, GM00220 and GM03700, derived from Menkes patients. The Menkes gene encodes a membrane pump responsible for copper exportation. Our results suggest that Menkes protein is a potential target of arsenite.  (+info)

Asp45 is a Mg2+ ligand in the ArsA ATPase. (8/791)

The ATPase activity of ArsA, the catalytic subunit of the plasmid-encoded, ATP-dependent extrusion pump for arsenicals and antimonials in Escherichia coli, is allosterically activated by arsenite or antimonite. Magnesium is essential for ATPase activity. To examine the role of Asp45, mutants were constructed in which Asp45 was changed to Glu, Asn, or Ala. Cells expressing these mutated arsA genes lost arsenite resistance to varying degrees. Purified D45A and D45N enzymes were inactive. The purified D45E enzyme exhibited approximately 5% of the wild type activity with about a 5-fold decrease in affinity for Mg2+. Intrinsic tryptophan fluorescence was used to probe Mg2+ binding. ArsA containing only Trp159 exhibited fluorescence enhancement upon the addition of MgATP, which was absent in D45N and D45A. As another measure of conformation, limited trypsin digestion was used to estimate the surface accessibility of residues in ArsA. ATP and Sb(III) synergistically protected wild type ArsA from trypsin digestion. Subsequent addition of Mg2+ increased trypsin sensitivity. D45N and D45A remained protected by ATP and Sb(III) but lost the Mg2+ effect. D45E exhibited an intermediate Mg2+ response. These results indicate that Asp45 is a Mg2+-responsive residue, consistent with its function as a Mg2+ ligand.  (+info)

Sodium arsenite exposure at concentration >5 M may induce embryotoxic and teratogenic effects in animal models. several downstream focuses on that control cell survival and apoptosis. Furthermore, the whole core transcription element circuitry that control self-renewal of mouse ESC (Stat3-P-Tyr705, April4, Sox2 and Nanog) was strongly down-regulated by sodium arsenite (4 M) exposure. This was adopted by G2/M police arrest and induction of the mitochondrial apoptotic pathway that might become suppressed by caspase-9 and caspase-3 inhibitors. In contrast to mouse ESC D-(+)-Xylose supplier with very low endogenous IL6, mouse neural come/precursor cells (C17.2 clone immortalized by and and [18, 19]; and [20]; and [21], which could induce reprogramming fibroblasts to pluripotency. The triumvirate D-(+)-Xylose supplier of transcription factors April4, Nanog and Sox2 takes on the fundamental part in gene regulations, frequently presenting multiple localised sites in the regulatory locations of the ...
DOSE DEPENDENT EFFECTS OF SODIUM ARSENITE ON NF-kB AND INTERLEUKIN-8 IN BRONCHIAL EPITHELIAL CELLS A Thesis Submitted to the Faculty In partial fulfillment of the requirements for the degree of Doctor of Philosophy in Pharmacology and Toxicology by Robert Ronald Roussel DARTMOUTH COLLEGE Hanover, New Hampshire July 31, 2000 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission ...
TY - JOUR. T1 - Arsenite exposure in human lymphoblastoid cell lines induces autophagy and coordinated induction of lysosomal genes. AU - Bolt, Alicia M.. AU - Douglas, Randi M.. AU - Klimecki, Walter T.. PY - 2010/11/30. Y1 - 2010/11/30. N2 - Chronic exposure to inorganic arsenic is associated with diverse, complex diseases, making the identification of the mechanism underlying arsenic-induced toxicity a challenge. An increasing body of literature from epidemiological and in vitro studies has demonstrated that arsenic is an immunotoxicant, but the mechanism driving arsenic-induced immunotoxicity is not well established. We have previously demonstrated that in human lymphoblastoid cell lines (LCLs), arsenic-induced cell death is strongly associated with the induction of autophagy. In this study we utilized genome-wide gene expression analysis and functional assays to characterize arsenic-induced effects in seven LCLs that were exposed to an environmentally relevant, minimally cytotoxic, ...
Arsenic can induce reactive oxygen species (ROS) leading to oxidative stress and carcinogenesis. Bladder is one of the major target organs of arsenic, and cyclooxygenase-2 (COX-2) may play an important role in arsenic-induced bladder cancer. However, the mechanism by which arsenic induces COX-2 in bladder cells remains unclear. This study aimed at investigating arsenic-mediated intracellular redox status and signaling cascades leading to COX-2 induction in human uroepithelial cells (SV-HUC-1). SV-HUC-1 cells were exposed to sodium arsenite and COX-2 expression, mitogen-activated protein kinase (MAPK) phosphorylation, glutathione (GSH) levels, ROS induction and Nrf2 expression were quantified. Our results demonstrate that arsenite (1-10 μM) elevates COX-2 expression, GSH levels, ROS and Nrf2 expression. Arsenite treatment for 24h stimulates phosphorylation of ERK and p38, but not JNK in SV-HUC-1 cells. Induction of Cox-2 mRNA levels by arsenite was attenuated by inhibitors of ERK, p38 and JNK. Arsenite
Arsenite has long been known as a human carcinogen. The hypoxia-inducible factor-1 (HIF-1) is an important transcription factor upon hypoxia and other stresses, which is proved to be associated with the pathogenesis of several cancers. Although hypoxia-inducible factor-1α (HIF-1α) has been reported to be activated by arsenite both in vivo and in vitro, the detailed molecular mechanisms leading to HIF-1α expression and activation due to arsenite exposure have not been well understood. In the present study, we found that arsenite is able to induce HIF-1α protein accumulation in both mouse epidermal Cl41 cells and mouse embryonic fibroblasts (MEFs). Knockout of p85α in MEFs (p85α−/−) led to the deficiency of HIF-1α protein accumulation induced by arsenite exposure, suggesting that p85α is crucial for arsenite-induced stabilization of HIF-1α protein. At the same time, our studies also show that p85a exhibited an inhibition of HIF-1α protein degradation via PI-3K/Akt-dependent pathway ...
Epidemiology studies have established a strong link between lung cancer and arsenic exposure. Currently, the role of disturbed cellular energy metabolism in carcinogenesis is a focus of scientific interest. Hypoxia inducible factor-1 alpha (HIF-1A) is a key regulator of energy metabolism, and it has been found to accumulate during arsenite exposure under oxygen-replete conditions. We modeled arsenic-exposed human pulmonary epithelial cells in vitro with BEAS-2B, a non-malignant lung epithelial cell line. Constant exposure to 1 µM arsenite (As) resulted in the early loss of anchorage-dependent growth, measured by soft agar colony formation, beginning at 6 weeks of exposure. This arsenite exposure resulted in HIF-1A accumulation and increased glycolysis, similar to the physiologic response to hypoxia, but in this case under oxygen-replete conditions. This
Results: Plasma membrane (p< 0.001) and acrosome integrity (p< 0.05) of the spermatozoa were significantly reduced in sodium arsenite group compared to the control. In silymarin + sodium arsenite group, silymarin was able to significantly (p< 0.001) ameliorate the adverse effects of sodium arsenite on these sperm parameters compared to sodium arsenite group. The incubation of sperm for 180 min (control group) showed a significant (p< 0.001) decrease in acrosome integrity compared to the spermatozoa at 0 hour. The application of silymarin alone for 180 min could also significantly (p< 0.05) increase sperm acrosome integrity compared to the control ...
The down-flow hanging sponge (DHS) reactor was used for continuous As removal treatment of As-contaminated water. The treatment scheme was: 1) As(III) in contaminated water is oxidized by arsenite-oxidizing bacteria fixed in the sponges in the reactor; 2) Fe(II) naturally existing in water is oxidized by dissolved oxygen; 3) Fe(III) is precipitated as iron hydroxide and As(V) is co-precipitated with the iron hydroxide; and finally 4) the co-precipitates are fixed in the sponges. This system could remove As from As-contaminated water on a small scale and at low cost. The results showed that, after using the DHS reactor, As and Fe concentrations in the treated water were lower than water quality standards for drinking water when Fe(II) concentration in influent was lower than 10 mg/L and the Fe/As ratio was higher than 6.67-8.42, with dependence on the Fe concentration. Additionally, even if Fe concentration is higher than 10 mg/L, the treatment system is still applicable if pH of the influent is ...
Sigma-Aldrich offers abstracts and full-text articles by [Chien-Te Chou, Hong-Ting Lin, Pai-An Hwang, Shang-Ta Wang, Cheng-Hong Hsieh, Deng-Fwu Hwang].
for toxicological evaluation and was selected for immunotoxicity studies by the chemical manager. The purpose of the range-finding study was to determine the doses of sodium arsenite to be used in an immuntoxicology protocol.. The range-finding studies were conducted in female B6C3F1 mice. The animals were administered sodium arsenite as a pellet implanted subcutaneously. The sodium-meta arsenite was purchased from Sigma Chemical Company and pelletized by Innovative Research of America. The pellets were prepared using 1, 3 and 6 mg/pellet as recommended by the producer to obtain the desired blood levels of 100, 300 and 600 ng/ml. These doses proved highly toxic and the pellets were then prepared at 0.01, 0.03 and 0.06 mg/pellet. Two studies were carried out in completing the range-finding protocol. The pellets were implanted on day 1 and the assay evaluations were performed on day 15. Dose levels of 0.01, 0.03 and 0.06 mg/pellet were used; however, the high dose of 0.06 mg/pellet resulted in the ...
23594 : Genome-wide DNA methylation analysis of sperm to explore the mechanism of hepatic tumor augmentation in the F2 by gestational arsenite exposure of F0 pregnant ...
Theres an article from 2004 about a vet who treated falcons diagnosed with CFS with Potassium Arsenite 0.05% leading to complete remission of symptoms...
S cerevisiae ACR3 protein: ACR - Arsenic Compounds Resistance; a putative membrane protein involved in Arsenite transport; has been sequenced
Heme oxygenase-1 is an inducible enzyme that catalyzes heme degradation and has been proposed to play a role in protecting cells against oxidative stress-related injury. We investigated the induction of heme oxygenase-1 by the tumor promoter arsenite in a chicken hepatoma cell line, LMH. We identified a heme oxygenase-1 promoter-driven luciferase reporter construct that was highly and reproducibly expressed in response to sodium arsenite treatment. This construct was used to investigate the role of mitogen-activated protein (MAP) kinases in arsenite-mediated heme oxygenase-1 gene expression. In LMH cells, sodium arsenite, cadmium, and heat shock, but not heme, induced activity of the MAP kinases extracellular-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. To examine whether these MAP kinases were involved in mediating heme oxygenase-1 gene expression, we utilized constitutively activated and dominant negative components of the ERK, JNK, and p38 MAP kinase signaling pathways. Involvement
TY - JOUR. T1 - Autophagy is the predominant process induced by arsenite in human lymphoblastoid cell lines. AU - Bolt, Alicia M.. AU - Byrd, Randi M.. AU - Klimecki, Walter T.. PY - 2010/5/1. Y1 - 2010/5/1. N2 - Arsenic is a widespread environmental toxicant with a diverse array of molecular targets and associated diseases, making the identification of the critical mechanisms and pathways of arsenic-induced cytotoxicity a challenge. In a variety of experimental models, over a range of arsenic exposure levels, apoptosis is a commonly identified arsenic-induced cytotoxic pathway. Human lymphoblastoid cell lines (LCL) have been used as a model system in arsenic toxicology for many years, but the exact mechanism of arsenic-induced cytotoxicity in LCL is still unknown. We investigated the cytotoxicity of sodium arsenite in LCL 18564 using a set of complementary markers for cell death pathways. Markers indicative of apoptosis (phosphatidylserine externalization, PARP cleavage, and sensitivity to ...
One of the key mediators of the antiviral and antiproliferative actions of interferon is double-stranded-RNA-dependent protein kinase (PKR). PKR activity is also involved in the regulation of cell proliferation, apoptosis and signal transduction. We have recently identified PACT, a novel protein activator of PKR, as an important modulator of PKR activity in cells in the absence of viral infection. PACT heterodimerizes with PKR and activates it by direct protein-protein interactions. Endogenous PACT acts as an activator of PKR in response to diverse stress signals, such as serum starvation and peroxide or arsenite treatment, and is therefore a novel, stress-modulated physiological activator of PKR. In this study, we have characterized the functional domains of PACT that are required for PKR activation. Our results have shown that, unlike the N-terminal conserved domains 1 and 2, the third conserved domain of PACT is dispensable for its binding of double-stranded RNA and inter action with PKR. ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Selected Publications Hur, H. and Reeder, R.J. (2016) Tungstate sorption mechanisms on boehmite: Systematic uptake studies and X-ray absorption spectroscopy analysis. J. Colloid. Interface Sci., 461, 249-260.. Sklute, E.C., Jensen, H.B., Rogers, A.D., Reeder, R.J. (2015) Morphological, structural, and spectral characteristics of amorphous iron sulfates, J. Geophys. Res. Planets, 120, 809-830.. Cerkez, E.B., Bhandari, N., Reeder, R.J., and Strongin, D.R. (2015) Coupled redox transformation of chromate and arsenite on ferrihydrite. Environ. Sci. Tech., 49, 2858-2866.. Szema, A.M., Reeder, R.J., Harrington, A.D., Schmidt, M., Liu, J., Golightly, M., Rueb, T., Hamidi, S.A., (2014) Iraq dust is respirable, sharp, and metal-laden and induces lung inflammation with fibrosis in mice via IL-2 upregulation and depletion of regulatory T cells. J. Occup. Environ. Med., 56, 243-251. Schmidt, M.P., Ilott, A.J., Phillips, B.L., and Reeder, R.J. (2014) Structural changes upon dehydration of amorphous calcium ...
Sigma-Aldrich offers abstracts and full-text articles by [Huan Xu, Xixi Zhou, Xia Wen, Fredine T Lauer, Ke Jian Liu, Laurie G Hudson, Lauren M Aleksunes, Scott W Burchiel].
Chemical genetics in yeast has shown great potential for clarifying the pharmacology of various drugs. Investigating these results from a systems perspective has uncovered many facets of natural chemical tolerance, but many cellular interactions of chemicals still remain poorly understood. To uncover previou
Yeast infections and vaginitis are common and easy to treat, but need a diagnosis from a professional. Make an appointment with us to discuss your symptoms
AAIDD (17) ability conception (5) ability constructs (2) ability domain cohesion (2) AC (45) academic attitudes (1) academic freedom (1) academic goal setting (1) academic interests (1) Academic Knowledge (1) academic motivation (2) academic values (1) accomodations (2) acculturation (2) achievement (87) ACT (1) ACT-R (1) adaptive behavior (11) ADHD (37) adpatations (1) advertising (3) aging (34) AimsWeb (2) AJT (2) AJT COGTESST (1) AJT COGTEST (2) alternate forms equivalence (2) alzheimers (7) American Indians (1) American Printing House for Blind (2) AP101 (15) AP101 Briefs (3) AP101 Reports (12) APA (13) APA Division 41 (1) applied psychometrics (29) aptitude (58) Arithmetic (2) as per person (1) ASB (8) ASI (1) ASIS (1) Aspergers (3) assessment (13) ASVAB (1) at risk (1) ATDR (1) ATI (2) Atkins (2) Atkins cases (33) attention (62) attentional control (45) atypical development (1) Australia (4) Austria (1) autism (18) background noise (1) BAI (1) BAIAD (1) basal ganglia (1) base rate (2) BAT ...
TY - JOUR. T1 - Both near ultraviolet radiation and the oxidizing agent hydrogen peroxide induce a 32-kDa stress protein in normal human skin fibroblasts. AU - Keyse, Stephen M.. AU - Tyrrell, Rex M.. PY - 1987/10/25. Y1 - 1987/10/25. N2 - We have analyzed the pattern of protein synthesis in solar near ultraviolet (334 nm, 365 nm) and near visible (405 nm) irradiated normal human skin fibroblasts. Two hours after irradiation we find that one major stress protein of approximately 32 kDa is induced in irradiated cells. This protein is not induced by ultraviolet radiation at wavelengths shorter than 334 nm and is not inducible by heat shock treatment of these cells. Although sodium arsenite, diamide, and menadione all induced a 32-kDa protein, they also induced the major heat shock proteins. In contrast, the oxidizing agent, hydrogen peroxide, induced the low molecular weight stress protein without causing induction of the major heat shock proteins. A comparison of the 32-kDa proteins induced by ...
Resistance to arsenite (As(III)) by cells is generally accomplished by arsenite efflux permeases from Acr3 or ArsB unrelated families. We analyzed the function of three Acr3 proteins from Corynebacterium glutamicum, CgAcr3-1, CgAcr3-2, and CgAcr3-3. CgAcr3-1 conferred the highest level of As(III) resistance and accumulation in vivo. CgAcr3-1 was also the most active when everted membranes vesicles from Escherichia coli or C. glutamicum mutants were assayed for efflux with different energy sources. As(III) and antimonite (Sb(III)) resistance and accumulation studies using E. coli or C. glutamicum arsenite permease mutants clearly show that CgAcr3-1 is specific for As(III). In everted membrane vesicles expressing CgAcr3-1, dissipation of either the membrane potential or the pH gradient of the proton motive force did not prevent As(III) uptake, whereas dissipation of both components eliminated uptake. Further, a mutagenesis study of CgAcr3-1 suggested that a conserved cysteine and glutamate are ...
1. A hypothesis is given concerning the action of urethan and arsenite on malignant growth. Two assumptionsares made:- (a) the enzyme system responsible for energy production in malignant tumours is working at maximal rate, contrary to the corresponding enzyme system in normal tissues. (b) a given concentration of urethan or ... read more arsenite blocks an equal part of the sensitive enzyme(s), by which a measurable effect of a small concentration of these inhibitors on the catabolism will only be obtained in case of maximal turnover rate. 2. Experiments with kidney and liver minces and with yeast have shown that indeed the inhibition caused by urethan and arsenite increases with increasing rate of respiration or fermentation. 3. If the enzyme concentration is not considered to be negligible as compared to the substrate concentration, as is done in the theory of and , the increase of inhibition with increasing turnover number can be made plausible by enzyme kinetics for the much simplified case ...
Mesoporous pellet adsorbent developed from mixing at an appropriate ratio of natural clay, iron oxide, iron powder, and rice bran was used to investigate the optimization process of batch adsorption parameters for treating aqueous solution coexisting with arsenate and arsenite. Central composite design under response surface methodology was applied for optimizing and observing both individual and interactive effects of four main influential adsorption factors such as contact time (24-72 h), initial solution pH (3-11), adsorbent dosage (0-20 g/L) and initial adsorbate concentration (0.25-4.25 mg/L). Analysis of variance suggested that experimental data were better fitted by the quadratic model with the values of regression coefficient and adjusted regression coefficient higher than 95%. The model accuracy was supported by the correlation plot of actual and predicted adsorption efficiency data and the residual plots. The Pareto analysis suggested that initial solution pH, initial adsorbate concentration,
Fowler , P A , Drake , A J , OShaughnessy , P J , Bhattacharya , S , Raab , A , Sinclair , K D , Feldmann , J & Meharg , A A 2016 , Comment on "Effects of Arsenite during Fetal Development on Energy Metabolism and Susceptibility to Diet-Induced Fatty Liver Diseases in Male Mice" and "Mechanisms Underlying Latent Disease Risk Associated with Early-Life Arsenic Exposure: Current Trends and Scientific Gaps" Environmental Health Perspectives , vol 124 , no. 6 , pp. A99-A99 . DOI: 10.1289/ehp. ...
Abstract: Antioxidant Actions of Dried Flower Extracts of Hibiscus sabdariffa L. On Sodium Arsenite - Induced Oxidative Stress in Rats
Influential parameters to produce iron-coated ZSM-5 zeolite including iron loading concentration, calcination temperature and time, synthesis temperature and mixing intensity were investigated. Results showed that the optimized iron-coated ZSM-5 nano-adsorbent is produced if 40 wt.% iron loading concentration at 30 °C synthesis temperature under 60 rpm mixing intensity and 400 °C calcination temperature for 3 hrs are applied. Also, optimization of some parameters such as pH, adsorbent dose, contact time and temperature for removal of arsenite from aqueous solution with 400 μg/L initial concentration was considered. Evidently, the highest adsorption of arsenite occurred under pH 7, adsorbent dose 2.5 g/L, contact time 30 min and temperature 40 °C. Kinetic and isotherm models studied showed that arsenite adsorption by iron-coated ZSM-5 follows pseudo-third order equation and well fitted with Langmuir isotherm model. Evaluation showed that the optimized iron-coated ZSM-5 zeolite is able to ...
Learn more about Chemical Stress Test at Reston Hospital Center DefinitionReasons for TestPossible ComplicationsWhat to ExpectCall Your Doctorrevision ...
When cells are subjected to stress, such as heat shock, genes associated with growth are downregulated and expression of proteins believed to increase cellular survival, such as HSP70, are selectively upregulated. In eukaryotes, this regulation operates at multiple levels: transcription initiation may be enhanced via factors specific for heat shock-genes, whereas for other genes there is selective inhibition of splicing and nuclear export of mRNAs (Bond, 2006).. Stress also influences the fate of cytoplasmic mRNA in eukaryotes. Treatment of mammalian cells with a variety of stresses, such as arsenite or heat shock, results in the relocation of most mRNAs from polysomes to cytoplasmic foci called stress granules (Kedersha et al., 1999). These contain the small ribosomal subunit (Kedersha et al., 2002), the translation-initiation factors eIF2, eIF3, eIF4E and eIF4G (Kedersha et al., 2002; Kimball et al., 2003), the poly(A)-binding protein (Kedersha et al., 1999), DHH1 (also known as DDX6, Rck, ...
This picture was taken by me a long time ago (6 years, i think). Contrary to the name of the blog, this is not GFP. To take this picture, I performed Immunofluorescence experiment. The procedure is as follows: Cells are treated with whatever you are invetigating (in this case - cells were treated with 0.5mM arsenite…
The influence of ethylenediaminetetraacetic acid (EDTA) and chemical species on arsenic accumulation in aquatic floating macrophyte Spirodela polyrhiza L. (duckweed) was investigated. The uptake of inorganic arsenic species (arsenate; As(V) and arsenite; As(III)) into the plant tissue and their adsorption on iron plaque of plant surfaces were significantly (p,0.05) higher than those of organic species (monomethylarsonic acid (MMAA) and dimethylarsinic acid (DMAA)). The addition of EDTA to the culture media increased the uptake of As(V) and As(III) into the plant tissue though the MMAA and DMAA uptake were not affected. About 46% of the inorganic arsenic species were desorbed or mobilized from iron plaque by EDTA. Desorption of organic arsenic species was not affected by EDTA addition because the co-precipitation occurs only with inorganic species. Phosphate uptake was not affected by EDTA though its concentration in citrate-bicarbonate-EDTA (CBE)-extract was much higher than that of plant ...
In molecular biology, the ars operon is an operon found in several bacterial taxon. It is required for the detoxification of arsenate, arsenite, and antimonite. This system transports arsenite and antimonite out of the cell. The pump is composed of two polypeptides, the products of the arsA and arsB genes. This two-subunit enzyme produces resistance to arsenite and antimonite. Arsenate, however, must first be reduced to arsenite before it is extruded. A third gene, arsC, expands the substrate specificity to allow for arsenate pumping and resistance. ArsC is an approximately 150-residue arsenate reductase that uses reduced glutathione (GSH) to convert arsenate to arsenite with a redox active cysteine residue in the active site. ArsC forms an active quaternary complex with GSH, arsenate, and glutaredoxin 1 (Grx1). The three ligands must be present simultaneously for reduction to occur. ArsA and ArsB form an anion-translocating ATPase. The ArsB protein is distinguished by its overall hydrophobic ...
RNA helicases are vital components of various pathways for post-transcriptional gene regulation. TgHoDI is a newly identified member of the DEAD-box RNA helicase family in Toxoplasma gondii. This study was aimed at characterizing the function of TgHoDI in post-transcriptional regulation during various stages of the parasite life cycle. Through immunofluorescence analyses, TgHoDI was found to colocalize with mRNA aggregates in cytoplasmic foci. Furthermore, colocalization studies of TgHoDI with PABPC3 identified these proteins as components of RNA aggregates known as stress granules. Cycloheximide and arsenite treatment of extracellular parasites revealed the presence of mechanisms involved in the determination of mRNA fate in Toxoplasma. Also, TgHoDI was found to functionally compliment the temperature sensitive phenotype in Δdhh1 yeast, validating its role in stress response. These findings confirm that TgHoDI is a vital component in the formation of stress granules and possibly involved in the
Base excision repair (BER) is crucial for development and for the repair of endogenous DNA damage. However, unlike nucleotide excision repair, the regulation of BER is not well understood. Arsenic, a well-established human carcinogen, is known to produce oxidative DNA damage, which is repaired primarily by BER, while high doses of arsenic can also inhibit DNA repair. However, the mechanism of repair inhibition by arsenic and the steps inhibited are not well defined. To address this question we have investigated the regulation of DNA polymerase β (Pol β) and AP endonuclease (APE1), in response to low, physiologically relevant doses of arsenic. GM847 lung fibroblasts and HaCaT keratinocytes were exposed to sodium arsenite, As(III), and mRNA, protein levels and BER activity were assessed. Both Pol β and APE1 mRNA exhibited significant dose-dependant down regulation at doses of As(III) above 1 µM. However, at lower doses Pol β mRNA and protein levels, and consequently, BER activity were ...
According to recent reports, millions of people across the globe are suffering from arsenic (As) toxicity. Arsenic is present in different oxidative states in the environment and enters in the food chain through soil and water. In the agricultural field, irrigation with arsenic contaminated water, that is, having a higher level of arsenic contamination on the top soil, which may affects the quality of crop production. The major crop like rice (Oryza sativa L.) requires a considerable amount of water to complete its lifecycle. Rice plants potentially accumulate arsenic, particularly inorganic arsenic (iAs) from the field, in different body parts including grains. Different transporters have been reported in assisting the accumulation of arsenic in plant cells; for example, arsenate (AsV) is absorbed with the help of phosphate transporters, and arsenite (AsIII) through nodulin 26-like intrinsic protein (NIP) by the silicon transport pathway and plasma membrane intrinsic protein aquaporins. Researchers and
Information about the open-access article Arsenic accumulation in rice: Consequences of rice genotypes and management practices to reduce human health risk in DOAJ. DOAJ is an online directory that indexes and provides access to quality open access, peer-reviewed journals.
I was told that someone could die of arsenic toxicity from eating a lot of shrimps/prawns. Im sure a lot means A LOT. Could you explain how this could happen and what happens in the system, and how to avoid/cure it? Thanl you ...
Harmonised classification and labelling is a legally binding classification and labelling for a substance, agreed at European Community level. Harmonisation is based on the substances physical, toxicological and eco-toxicological hazard assessment. The Hazard classification and labelling section uses the signal word, pictogram(s) and hazard statements of the substance under the harmonised classification and labelling (CLH) as its primary source of information.. If the substance is covered by more than one CLH entry (e.g. disodium tetraborate EC no. 215-540-4, is covered by three harmonisations: 005-011-00-4; 005-011-01-1 and 005-011-02-9), CLH information cannot be displayed in the InfoCard as the difference between the CLH classifications requires manual interpretation or verification. If a substance is classified under multiple CLH entries, a link to the C&L Inventory is provided to allow users to view CLH information associated with the substance and no text is automatically ...
2000, describes a method for removing arsenic from fly ash, in which arsenic is recovered as scorodite. The first treatment stage of the arsenic-containing material is the oxidation of trivalent arsenic (As(lll)) into pentavalent arsenic (As(V)) with a gas containing sulphur dioxide and oxygen in oxidising conditions, in which arsenic does not precipitate. After this, arsenic is precipitated in atmospheric conditions, in which the Fe(III)/As(V) mole ratio is specified as 1. Precipitation is carried out either in one or several stages, but precipitation as scorodite demands the over-saturation of the solution, which is achieved by recycling scorodite crystals to the first precipitation reactors and simultaneously neutralising the suspension. A beneficial pH range is around 1-2 and this is maintained by feeding a suitable neutralising agent into the precipitation stage. In these conditions, arsenic can be precipitated to the level of 0.5 g/l. The final arsenic removal to a level below 0.1 mg/l is ...
Spirulina is blue - green algae that grow both in fresh and salt water. For many years, the alga has used for various health purposes around the world. This nutrient-rich alga is a natural supplement that provides amazing health benefits to the users. Here are the benefits of Spirulina.. #1. Detoxing properties The studies show that regular intake of Spirulina can effectively detox heavy metals such as Arsenic. It has been demonstrated that chronic Arsenic toxicity is common in most parts of the world. Millions of people consume Arsenic through drinking water. Apparently, there is no specific treatment for arsenic toxicity. However, the clinical trials have proven that Spirulina can significantly reduce the level of toxicity in the body.. #2. Packed with essential nutrients Our bodies require essential nutrients for proper functioning. Fortunately, Spirulina is rich in essential nutrients and low in calories. The algae manufacture its food through photosynthesis thus making it rich in vital ...
A meta description is an HTML tag in the HTML code of your website, which allows you to customize a section of text that describes the page itself. It plays a role in how your page is seen by search engine crawlers, and how it appears in SERPs
Skin is the largest organ of the body.It is a major barrier between the body and the external environment. Main Functions of skin: Protection: It forms a protection layer against mechanical/thermal/chemical stress, infection Sensation: skin contains touch, pressure, pain and … Read More … ...
Skin is the largest organ of the body.It is a major barrier between the body and the external environment. Main Functions of skin: Protection: It forms a protection layer against mechanical/thermal/chemical stress, infection Sensation: skin contains touch, pressure, pain and … Read More … ...
Define antimonite. antimonite synonyms, antimonite pronunciation, antimonite translation, English dictionary definition of antimonite. n an oxyanion of antimony or a salt containing an oxyanion of antimonya mineral from which antimony is obtained
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Arsenic atom in PDB 1j9b: Arsenate Reductase+0.4M Arsenite From E. Coli
Mangrove wetlands serve as both a sink and source for arsenic (As), as mangrove plants are able to uptake and accumulate As. The present study used pot experiments to evaluate As accumulation and translocation in mangrove (Aegiceras corniculatum L.) seedlings grown in As contaminated soils. Results indicated that A. corniculatum seedlings grew normally under As stress with minute growth inhibition and biomass reduction at different As treatment concentrations in a range of 0-150 mg·kg−1. As concentrations in roots, stems and leaves were increased with increasing As treatment concentrations, but As accumulated mainly in roots, with accumulation rates of 74.54%-89.26% of the total As accumulation. In particular, relatively high bioconcentration factor (BCF) in root (2.12-1.79), low BCF in stem (0.44-0.14) and leaf (0.06-0.01), and thereby a low translocation factor (TF) in stem/root (0.21-0.08) and leaf/root (0.02-0.008) were observed. These results demonstrated that A. corniculatum is an As excluder
Rep. Rosa DeLauro (D-CT) introduced a bill in Congress on Thursday which would limit the amount of inorganic arsenic permitted in rice and rice-based foods. The R.I.C.E (Reducing food-based Inorganic Compounds Exposure) Act would require the Food and Drug Administration to set a maximum permissible level of inorganic arsenic in rice and food containing rice. "Inorganic arsenic… Continue Reading. ...
Arsenic is a well-known human carcinogen that affects millions of people worldwide, but the underlying mechanisms of carcinogenesis are unclear. Several epidemiological studies have suggested increased prostate cancer incidence and mortality due to exposure to arsenic. Due to lack of an animal model of arsenic-induced carcinogenesis, we used a prostate epithelial cell culture model to identify a role for mitochondria in arsenic-induced prostate cancer. Mitochondrial morphology and membrane potential was impacted within a few hours of arsenic exposure of non-neoplastic prostate epithelial cells. Chronic arsenic treatment induced mutations in mitochondrial genes and altered mitochondrial functions. Human non-neoplastic prostate epithelial cells continuously cultured for seven months in the presence of 5 µM arsenite showed tumorigenic properties in vitro and induced tumors in SCID mice, which indicated transformation of these cells. Protein and mRNA expression of subunits of mtOXPHOS complex I ...
Acidiphilium multivorum (strain DSM 11245 / JCM 8867 / AIU301) is an acidophilic, aerobic, anoxygenic, phototrophic, Gram-negative bacterium isolated from pyritic acid mine drainage. A.multivorum has high ability of resistance to various metals under acidic condition, and require high acidity for growth. It exhibits tolerance towards some heavy metal ions like nickel, zinc, cadmium and copper, and resistance to arsenate and arsenite. A. multivorum has zinc-chelated-bacteriochlorophylls (Zn-Bchl) a rather than Mg-Bchl a as the major photopigment. Among the mesoacidophilic bio-mining heterotrophs, A. multivorum has the unique ability to oxidize arsenite to arsenate as well as the capability to utilize a wide range of organic compounds for growth. These features make this bacterium a suitable host for carrying purpose specific genes for developing bioleaching operations. Current trends in biohydrometallurgy emphasize genetic engineering of bio-mining bacteria to achieve further success in this ...
Presented by M di-T. CUPRUM ARSENICOSUM.. Arsenite of copper; CuHAsO3 (Scheeles Green). (Some of the cases of poisoning may be due to Schweinfurt Green, a double salt of arsenite and acetate of copper).. Preparation, Triturations.. Authorities. 1, Dr. James Blakely, Hahn. Month., 3, 571 (Blackelys provings, potency given in brackets after each symptom); [Symptoms without authority are so given in the original.] 2, Dr. R. C. Smedley, ibid.; 3, Dr. C. W. Boyce, ibid.; 4, Dr. George, S. Foster, ibid.; 5, Mr. L., ibid.; 6, A. Chevallier, Le Cuivre, p. 47, poisoning of a child by licking a shell covered with Scheeles Green, from J. de Connaiss. M d., 1840; 7, same, effects on workmen; 8, same, poisoning of three persons by strawberries from a vessel colored with Cup. arsen.; 9, Finnel, a woman took some to commit suicid, N. Y. Med. Rec., 4, 16; 10, Leale, a woman took ten cents worth, same, p. 70; 11, Br. and F. Med.-Chir. Rev., 1851, poisoning (three cases) by confectionery; 12, Fergus, ...
Arsenite of Copper ; Scheeles Green. Cu H As O3.. Provings, by Blakely (Hah. Mo., vol. 3, p. 571) assisted by Smedley, Boyce and Foster, and toxicological reports, contained in Encyclop dia, vol. 4, p. 23. The pure arsenite of copper is prepared by trituration.. CLINICAL AUTHORITIES.. - Vomiting, South, Raues Rec., 1875, p. 136 ; Lilienthal, Allg. Hom. Ztg., vol. 106, p. 207 ; Pains in bowels, (2 cases) Marsden, Raues Rec., 1874, p. 203 ; Neuralgic pains in bowels, Hale, Hales Therap., P. 44 ; Diarrh a, Marsden, Raues Rec., 1874, p. 188, Farrington, Allg. Hom. Ztg., Bd. 103, p. 102 ; Choleraic diarrh a, Holcombe, Raues Rec., 1874, p. 197 ; Pain in ovary, Marsden, Raues Rec., 1874, p. 228 ; Spasmodic uterine pains, Marsden, Raues Rec. 1874, p. 239 ; Vomiting during pregnancy, Marsden, Raues Rec., 1874, p. 239 ; Cardiac chorea, Hale, Hales Therap., p. 44.. MIND. [1] [Cupr. ar.]. A Kind of intoxication. Confusion of ideas. Intense anguish of mind and body. SENSORIUM. [2] [Cupr. ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
Number of succussion strokes affects effectiveness of ultra-high-diluted arsenic on in vitro wheat germination and polycrystalline structures obtained by droplet evaporation method - Società Italiana di Medicina Antroposofica - Lucietta Betti, Grazia Trebbi, Maria Olga Kokornaczyk, Daniele Nani, Maurizio Peruzzi, Giovanni Dinelli, Paolo Bellavite and Maurizio Brizzi Homeopathy (2016) -, 1- 8 2016 The Faculty of Homeopathy. Published by Elsevier Ltd. All rights reserved.
Arsenite methyltransferase is an enzyme that in humans is encoded by the AS3MT gene. GRCh38: Ensembl release 89: ENSG00000214435 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000003559 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Lin S, Shi Q, Nix FB, Styblo M, Beck MA, Herbin-Davis KM, Hall LL, Simeonsson JB, Thomas DJ (2002). "A novel S-adenosyl-L-methionine:arsenic(III) methyltransferase from rat liver cytosol". J. Biol. Chem. 277 (13): 10795-803. doi:10.1074/jbc.M110246200. PMID 11790780. "Entrez Gene: AS3MT arsenic (+3 oxidation state) methyltransferase". Dheeman DS, Packianathan C, Pillai JK, Rosen BP (2014). "Pathway of human AS3MT arsenic methylation". Chemical Research in Toxicology. 27 (11): 1979-89. doi:10.1021/tx500313k. PMC 4237493 . PMID 25325836. Hernández A, Xamena N, Sekaran C, Tokunaga H, Sampayo-Reyes A, Quinteros D, Creus A, Marcos R (2008). "High arsenic metabolic efficiency in AS3MT287Thr allele carriers". Pharmacogenet. Genomics. 18 ...
Data on 6,500 pesticides, insecticides and herbicides including toxicity, water pollution, ecological toxicity, uses and regulatory status.
04.HE.20 04 - OXIDES (Hydroxides, V[5,6] vanadates, arsenites, antimonites, bismuthites, sulfites, selenites, tellurites, iodate ...
Greenmedinfo.com - Natural Health Resource - The worlds most widely referenced, open access, natural medicine database, with 30,000+ study abstracts and growing daily
Full data to be presented at NAASO meeting October 20-24, SAN DIEGO, CA, July 24, 2007 Orexigen Therapeutics, Inc. (NASDAQ OREX), a biopharmaceutical company fo
TY - JOUR. T1 - Genetic polymorphisms in glutathione S-transferase (GST) superfamily and risk of arsenic-induced urothelial carcinoma in residents of southwestern Taiwan. AU - Hsu, Ling I.. AU - Chen, Wu Ping. AU - Yang, Tse Yen. AU - Chen, Yu Hsin. AU - Lo, Wann Cheng. AU - Wang, Yuan Hung. AU - Liao, Ya Tang. AU - Hsueh, Yu Mei. AU - Chiou, Hung Yi. AU - Wu, Meei Maan. AU - Chen, Chien Jen. PY - 2011. Y1 - 2011. N2 - Background: Arsenic exposure is an important public health issue worldwide. Dose-response relationship between arsenic exposure and risk of urothelial carcinoma (UC) is consistently observed. Inorganic arsenic is methylated to form the metabolites monomethylarsonic acid and dimethylarsinic acid while ingested. Variations in capacity of xenobiotic detoxification and arsenic methylation might explain individual variation in susceptibility to arsenic-induced cancers. Methods. To estimate individual susceptibility to arsenic-induced UC, 764 DNA specimens from our long-term follow-up ...
The German Federal Institute for Risk Assessment (BfR) is recommending that methods to minimise the levels of inorganic arsenic in rice products should be investigated in the light of recent dietary exposure studies in the German population.. Analyses of rice and rice products in Germany have shown that many contain relatively high concentrations of carcinogenic inorganic arsenic. Some products, such as rice cakes, were found to contain more inorganic arsenic than rice grains, but the reason for this is not known.. The BfR has calculated the intake of toxic inorganic arsenic compounds through the consumption of rice and rice products in Germany and concludes that they could make a significant contribution to the overall intake, especially in children. "Because inorganic arsenic compounds are classified as carcinogenic for humans, foodstuffs should only contain as little as reasonably achievable," says BfR President Professor Dr Dr Andreas Hensel.. The Institute is advising food manufacturers to ...
Arsenic toxicity is species-dependent for mammals and is transformed to other metabolites in various organs. In order to understand its metabolism, in-vitro simulation experiments were set up for various organ tissues. As(V) was transformed to As(III), while DMA(V) was changed to DMAS in sheep rumen, but the suspected MA-V) was not formed. This is different for the incubation of seaweed in rumen fluid, which points to a different unknown source for MA(V). In another study, dog MDCK kidney cells showed that inorganic arsenicals were methylated in kidney cells while the exposed DMAS was only oxidized to DMA(V) in kidney cells, and the exposed DMA(V) rarely transformed. The toxicities of arsenicals in dog kidney cells is dependant upon the uptake rates and the transformation of arsenicals. DMAS was as toxic as the inorganic arsenic species and an order to magnitude more toxic than the oxo-species. Methylated arsenic species can accumulate in hair and wool and can be used as a biomarker for arsenic ...
Arsenic is a highly poisonous metallic element in the nitrogen family of group Va in the periodic table. Symbol As; aomic number 33; atomic mass 74.9216; melting point ca 817°C; sublimation point ca 613°C; specific gravity 6.80 or 7.004; 5.73; valence -3, 0, +3, or +5.; electronic config. [Ar]3d104s24p3. It appears in three allotropic forms, yellow, black, and gray. The stable form is a brittle, steel-gray hexagonal solid that oxidizes rapidly in air, and at high temperatures burns to form a white cloud of arsenic trioxide. Arsenic and some arsenic compounds sublime when heated and convert to gaseous form. Arsenic is an omnipresent element as arsenic minerals in the various lithosphere or fossil fuels including realgar, orpiment, and arsenopyrite. It is prepared commercially from arsenical pyrites (sulfide ores) by condensation of sublimed gas and by the reduction of white arsenic with carbon. This element contributes hardness and is used in preparing alloys for hard and corrosion-resistant ...
Manufacturer and exporter miscellaneous chemicals, aluminum potassium sulfate, calcium arsenite, calcium borate, calcium boride, calcium chloride hydrate reagent, calcium molybdate, calcium permanganate, calcium salts, inorganic chemicals, potassium pentaborate, potassium pyrophosphate, potassium tetraborate, pyrophosphates (salts), sodium arsenate, sodium arsenite, sodium bisulfate, sodium borate, sodium cacodylate, sodium chromate, sodium cobaltinitrite, sodium copper cyanide. Bhagwati Chemicals Vadodara
What is arsenic and where do you find it?. Arsenic is a naturally occurring element that is widely distributed in the earths crust. Arsenic can exist in several oxidation states with other elements as inorganic or organic arsenic. It is a silvery-gray, semimetallic substance that tarnishes in air. Inorganic and organic arsenic compounds are white in color, and have no smell or special taste. Inorganic arsenic occurs naturally in certain types of soils and rock formations. Inorganic arsenic compounds are mainly used as a preservative in pressure-treated wood to make it resistant to rotting and decay. Organic arsenic compounds are used to make insect killers, weed killers, and fungicides.. Who is exposed to arsenic?. Anyone can be exposed to arsenic since low levels of arsenic are present in soil, water, food, and air. People are primarily exposed to arsenic through eating food (fish and shellfish), drinking water, or breathing air containing arsenic. If wells are drilled in areas where naturally ...
TY - ABST. T1 - Determination of inorganic arsenic by fast anion exchange HPLC-ICP-MS. AU - Jerše, Ana. AU - Høgsbro, Julie Storm. AU - Larios, Raquel. AU - Sloth, Jens J.. PY - 2019. Y1 - 2019. KW - Inorganic arsenic. KW - HPLC. KW - ICP-MS. KW - Food. KW - Feed. M3 - Conference abstract for conference. ER - ...
Read "Arsenic-induced changes in growth and antioxidant metabolism of fenugreek, Russian Journal of Plant Physiology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
A persistent progressive non-elevated red scaly or crusted plaque which is due to an intradermal carcinoma and is potentially malignant. Atypical squamous cells proliferate through the whole thickness of the epidermis. The lesions may occur anywhere on the skin surface or on mucosal surfaces. The cause most frequently found is trivalent arsenic compounds. Freezing, cauterization or diathermy coagulation is often effective. (From Rook et al., Textbook of Dermatology, 4th ed, pp2428-9). ...
Spirulina is blue - green algae that grow both in fresh and salt water. For many years, the alga has used for various health purposes around the world. This nutrient-rich alga is a natural supplement that provides amazing health benefits to the users. Here are the benefits of Spirulina.. #1. Detoxing properties The studies show that regular intake of Spirulina can effectively detox heavy metals such as Arsenic. It has been demonstrated that chronic Arsenic toxicity is common in most parts of the world. Millions of people consume Arsenic through drinking water. Apparently, there is no specific treatment for arsenic toxicity. However, the clinical trials have proven that Spirulina can significantly reduce the level of toxicity in the body.. #2. Packed with essential nutrients Our bodies require essential nutrients for proper functioning. Fortunately, Spirulina is rich in essential nutrients and low in calories. The algae manufacture its food through photosynthesis thus making it rich in vital ...
The noncarcinogenic effects of arsenic are reviewed. Included are its toxicity, threshold limit value compared with other toxic substances, use as a poison, arsenic levels in food, body burden in various tissues, the arsenic cycle in nature, signs and symptoms of arsenic poisoning in man and animals, enzymes inhibited by arsenic, blocking by arsenite of the conversion of malate to fumarate or vice
Gaia Health offers a different take on health. Although our articles are well researched and sources are provided, we accept no responsibility, including for the efficacy of the information or how the information may be used. In addition, the opinions expressed in articles on this site are solely those of the authors, and do not necessarily reflect the opinions of the owner(s) of this website. Please consult your doctor as your primary source of health information. ...
Complete information for ARSA gene (Protein Coding), Arylsulfatase A, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ARSA gene (Protein Coding), Arylsulfatase A, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Looking for online definition of dimethylarsinic acid in the Medical Dictionary? dimethylarsinic acid explanation free. What is dimethylarsinic acid? Meaning of dimethylarsinic acid medical term. What does dimethylarsinic acid mean?
In 2013 FSANZ released a survey on the levels of inorganic arsenic in a range of seaweed types and products containing seaweed, available in Australia.. The levels in most seaweed tested were below the regulatory limit for inorganic arsenic, with the exception of one hijiki seaweed sample. Because this sample was made up of two individual samples of the same brand of hijiki it may not be representative of all hijiki seaweed available for sale in Australia. Hijiki seaweed is also tested at the border for inorganic arsenic and compliance rates are high.. The New Zealand Ministry for Primary Industries (MPI) has also conducted tests on levels of arsenic in imported hijiki products and found some levels exceeding the regulatory limit for inorganic arsenic.. Using the information collected on the levels of inorganic arsenic in seaweed, dietary exposure to inorganic arsenic was estimated for the Australian population. Using the median inorganic arsenic concentrations in seaweed, the contribution of ...
TY - JOUR. T1 - Effect of trivalent arsenicals on cell proliferation in mouse and human microvascular endothelial cells. AU - Dodmane, Puttappa R.. AU - Arnold, Lora L. AU - Pennington, Karen L.. AU - Singh, Rakesh K. AU - Cardoso, Ana Paula Ferragut. AU - Cohen, Samuel Monroe. PY - 2015/5/1. Y1 - 2015/5/1. N2 - Chronic exposure to high levels of inorganic arsenic (iAs) has been associated with cancerous and non-cancerous health effects, including cardiovascular effects. However, the mechanism for a presumed toxic effect of arsenic on vascular tissue is not clear. Our working hypothesis is that inorganic trivalent arsenic and its methylated metabolites react with cysteine-containing cellular proteins and alter their function leading to adverse events such as cytotoxicity or proliferation. In this study, human microvascular endothelial cells (HMEC1) and mouse microvascular endothelial cells (MFP-MVEC) were exposed to arsenite (iAsIII), monomethylarsonous acid (MMAIII), or dimethylarsinous acid ...
TY - CHAP. T1 - Arsenic methylation capability and human health. AU - Hsueh, Yu Mei. AU - Huang, Yung Kai. AU - Chung, Chi Jung. PY - 2011/1/1. Y1 - 2011/1/1. N2 - The metabolism of inorganic arsenic takes place through a series of reactions, including reduction and oxidative methylation reactions. Biomethylation is considered a major detoxification pathway for inorganic arsenic. However, interspecies differences exist according to the efficiency with which inorganic arsenic is metabolized and excreted in urine. Intermediate trivalent methylated arsenic species are more cytotoxic and genotoxic than inorganic compounds. The arsenic methylation profile is influenced by demographic characteristics, lifestyle, diet and genetic susceptibility. Inefficient methylation is related to the risk of cancer and noncancer disease.. AB - The metabolism of inorganic arsenic takes place through a series of reactions, including reduction and oxidative methylation reactions. Biomethylation is considered a major ...
Inorganic arsenic is a human carcinogen that can target the liver, but its carcinogenic mechanisms are still unknown. Global DNA hypomethylation occurs during arsenic-induced malignant transformation in rodent liver cells. DNA hypomethylation can increase gene expression, particularly when occurring in the promoter region CpG sites, and may be a non-genotoxic mechanism of carcinogenesis. Thus, in the present study liver samples of male mice exposed to 0 (control) or 45 p.p.m. arsenic (as NaAsO2) in the drinking water for 48 weeks were analyzed for gene expression and DNA methylation. Chronic arsenic exposure caused hepatic steatosis, a lesion also linked to consumption of methyl-deficient diets. Microarray analysis of liver samples showed arsenic induced aberrant gene expression including steroid-related genes, cytokines, apoptosis-related genes and cell cycle-related genes. In particular, the expression of the estrogen receptor-α (ER-α), and cyclin D1 genes were markedly increased. RT-PCR and ...
The ArsA ATPase is the catalytic subunit of the pump protein, coupling the hydrolysis of ATP to the movement of arsenicals and antimonials through the membrane-spanning ArsB protein. Previously, we have shown the binding and hydrolysis of MgATP to ArsA to be a multi-step process in which the rate-limiting step is an isomerization between different conformational forms of ArsA. This isomerization occurs after product release, at the end of the ATPase reaction, and involves the return of the ArsA to its original conformation, which can then bind MgATP. ArsA possesses an allosteric site for antimonite [Sb(III)], the binding of which elevates the steady-state ATPase activity. We have used a transient kinetics approach to investigate the kinetics of ternary complex formation that lead to an enhancement in the ATPase activity. These studies revealed that ArsA exists in at least two conformational forms that differ in their ligand binding affinities, and that ATP favours one form and Sb(III) the other. ...
Silver Spring, MD (TFC) - An approved animal drug known as 3-Nitro® (roxarsone) was added to chicken feed prior to July 2011. This drug was known to contain an organic form of arsenic, which is known to be toxic, but indeed it is less toxic than inorganic arsenic. In 2011, the Food and Drug Administration admitted that roxarsone contained inorganic arsenic, which is a much more toxic compound. After discovering this, the FDAs Center for Veterinary Medicine and the Center for Food Safety and Applied Nutrition developed a new analytical method used to detect minute levels of arsenic in animal tissue.. It is important to note that organic arsenic is in the environment as a naturally occurring substance and can also be a contaminant in water, air, soil and various food products. In this case, the cause for concern is the organic arsenic found in 3-Nitro® that could transform into inorganic arsenic found in the chicken feed at high levels.. The FDA made a statement in the Product Safety ...
Hundreds of millions of people world-wide are exposed to unacceptable levels of arsenic in drinking water. This is a public health crisis because arsenic is a Group I (proven) human carcinogen. Human cells methylate arsenic to monomethylarsonous acid (MMAIII), monomethylarsonic acid (MMAV), dimethylarsinous acid (DMAIII), and dimethylarsinic acid (DMAV). Although the liver is the predominant site for arsenic methylation, elimination occurs mostly in urine. The protein(s) responsible for transport of arsenic from the liver (into blood), ultimately for urinary elimination are unknown. Human multidrug resistance protein 1 (MRP1/ABCC1) and MRP2 (ABCC2), are established arsenic efflux pumps, but unlike the related MRP4 (ABCC4), are not present at the basolateral membrane of hepatocytes. MRP4 is also found at the apical membrane of renal proximal tubule cells making it an ideal candidate for urinary arsenic elimination. In the current study, human MRP4 expressed in HEK293 cells reduced the ...
Author summary Zika virus (ZIKV) is transmitted to humans primarily through mosquito bites, but there have also been cases of sexual, perinatal, and suspected blood transfusion transmission. It has been associated with fetal malformations and neurological disorders in adults. The rising concern about this pathogen led the World Health Organization to declare it as a public health emergency of international concern regarding neurological disorders. There is an urgent global scientific effort underway to better understand ZIKV biology and define interactions that occur between the virus and the host cell. We evaluated how ZIKV infection counteracts the assembly of dynamic aggregates of RNA and proteins called stress granules (SGs). We observed that ZIKV blocks SG assembly induced by sodium arsenite (Ars), but not by sodium selenite or Pateamine A. We demonstrate that this difference is related to the ability of ZIKV to modulate the dephosphorylation of eIF2α via its phosphatase. Our work demonstrates
A family of 10 thermoresistant cell lines cloned from Chinese hamster cells transfected with a plasmid containing the structural gene for the small human Mr 27,000 heat shock protein (HSP27) was used to assess the putative role of this heat shock protein in chemoresistance. These cells express varying amounts of human HSP27 in addition to the normal level of endogenous hamster HSP27. As previously observed in the case of thermoresistance, a significant positive linear correlation (P , 0.05) was found between cell survival in response to doxorubicin and the total amount of HSP27 expressed. Some clones were also examined for resistance to other drugs and chemicals. A statistically significant increased survival relative to the parental cells was observed following treatment with daunorubicin (three clones studied), colchicine, vincristine, actinomycin D, hydrogen peroxide, and sodium arsenite (one clone studied). However, the clone which expressed the highest level of HSP27 was as sensitive as ...
The committee also identified several factors that could affect susceptibility to inorganic arsenic, including life stage when exposure occurs, genetic factors, sex, nutritional deficiencies, health status, smoking or alcohol consumption, or exposures to mixtures of chemicals. Based on the degree of evidence concerning these vulnerability factors, consideration should be given to whether dose-response analyses should focus on the population as a whole or involve separate assessments for the susceptible groups, the report says. In particular, consideration should also be given to early-life exposure and the risk of adverse health effects later in life, the report adds. EPAs current approach of assessing partial-lifetime exposures by prorating the risk equally regardless of age needs to be critically evaluated to determine whether it is appropriate for assessing inorganic arsenic ...
In January 2016, the EU imposed a maximum limit of inorganic arsenic on manufacturers in a bid to mitigate associated health risks. Researchers at the Institute for Global Food Security at Queens have found that little has changed since this law was passed and that 50 percent of baby rice food products still contain an illegal level of inorganic arsenic.. "This research has shown direct evidence that babies are exposed to illegal levels of arsenic despite the EU regulation to specifically address this health challenge. Babies are particularly vulnerable to the damaging effects of arsenic that can prevent the healthy development of a babys growth, IQ and immune system to name but a few," said Andy Meharg, lead author of the study and professor of plant and soil sciences at Queens.. Rice has, typically, 10 times more inorganic arsenic than other foods and chronic exposure can cause a range of health problems including developmental problems, heart disease, diabetes and nervous system ...
About Arsenic== Arsenic is the 33rd element which symbol is As in the periodic table and is a chemical element found in many mineral or in pure crystal forms. ,br> It is or was used in many industrial sectors as chemistry, electronics, metalworking, military, pharmaceutical, phytosanitary, mining, and others. ,br> Arsenic is also found in ground water, soil, air due to industrial or geological pollution and is found in many different forms and is often conjugated with sulfur and others metal.,br> Almost all arsenic products are toxic but the inorganic ones are more dangerous. The AsO3, also called arsenic trioxide or arsenite, is one of the most common form in the environment because of its important production in industry (around 50000 tones per year). Its also a soluble form which thus can be ingested by drinking water which contain some. It explains why its one of the most toxic form (until 500 times more toxic than pure arsenic). ,br>,br> Acute intoxication is caused by ingestion of a ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
The authors estimated the global burden of disease for bladder, lung, and skin cancers attributable to inorganic arsenic in food. The authors - 1) established dose response estimates by converting dose response estimates for water exposure to human dose; 2) estimated exposure using data on a common range of arsenic content for food crops grown in different parts of the world and dietary patterns in different parts of the world; 3) multiplied the dose-response slope factor with the estimated range of daily dietary inorganic arsenic exposure to characterize cancer risk; and 4) summed across different populations to estimate the global burden of a particular arsenic-induced cancer ...
This blood test is used to measure Arsenic levels. Arsenic is a common cause of acute poisoning through heavy metals. Arsenic enters the environment through the smelting of copper, zinc and lead and is released through the manufacturing of certain chemicals. Pesticides that contain arsenic, when manufactured, release arsine gas. Arsenic has been found in water supplies around the world, which has the potential to leach into seafood. Arsenic is found in rat poisoning, fungicides and products used to protect wood. Arsenic toxicity affects the blood, kidneys, skin, digestive tract and central nervous system ...
The aim of this paper is to report on the presence of chemical interferences in the quantitation of methylated arsenic species in human urine when using a method based on selective volatile arsine species generation, chromatographic separation, and hydride generation atomic absorption spectrometry HGAAS detection. An abnormal profile of...
Find all books from Musik: Samanta, Gautam - Preservation of Arsenic Species. At find-more-books.com you can find used, antique and new books, COMPARE results and immediately PURCHASE your selection at the best price. 9781843399698
The groundwater arsenicals have brought dreadful misery for the people residing in the endemic regions of West Bengal, India. Arsenic-related anomalies include ...
Chronic exposure to inorganic arsenic leads to an increased risk of cancer. A biological measurement was conducted in 153 private well owners and their families consuming water contaminated by inorganic arsenic at concentrations that straddle 10 μg/L.
The spectroscopic and photophysical properties of fluorescent probes comprising donor-acceptor derivatives comprising the boric acid group or a derivative of boric acid, B(OH)3 (or borate ion, BO(OH)2 −1), arsenious acid, H3 AsO3 (or arsenite ion, H2AsO3 −1), telluric acid, H6TeO6 (or tellurate ion, H5 TeO6 −1) or germanic acid, Ge(OH)6 (or germanate ion, GeO(OH)3 −1) are described. Method of using said probes are also provided.
Arsenicals are relatively weak poisons and the sisters being aware of this used two poisons, strychnine and cyanide, which dwarf arsenicals in their effectiveness. (1) Even though arsenic wasnt the agent of death arsenic and old lace has a much nicer artistic ring to it then strychnine and old lace or cyanide and old lace. Regardless, arsenic is a much feared material, resulting in what Ralph Zangaro calls arsenophobia. (2). No debate arsenic is a poison, but it has been used medicinally for more than 3000 years to treat human illnesses, including infectious diseases and malignancies. In recent times, Chinese researchers reported that arsenic trioxide induced remissions in patients with promyelocytic leukemia, a cancer of the blood, including those who had relapsed after other prior therapy. (3) In another report concerning a long-term study in China, doctors may appear to have safely and successfully treated patients with cancer of the blood and bone marrow with a combination of arsenic ...
A recent newsletter from EWC informed me that alot rice products have been found to contain high levels of arsenic, including baby rice. Further investigation showed me that Earths Best baby rice cereal was one of those tested and they showed to have very high levels of arsenic (higher than the accepted level found in water) This was terrifying to me as, like many others, this was my babys first food... and I continued to feed it to him until now. Yes, they are an organic company and they dont spray any pesticides, including arsenic on their food. However, there is arsenic in the soil that has been there for years from others using arsenic as a pesticide years ago and this gets into their products. Now the thing that disgusts me the most is that this organic company that says they care about health, has not recalled any of their rice containing products, including the baby rice. They continue to sell these products to unsuspecting parents who may be compromising their childrens health. Not ...
A recent newsletter from EWC informed me that alot rice products have been found to contain high levels of arsenic, including baby rice. Further investigation showed me that Earths Best baby rice cereal was one of those tested and they showed to have very high levels of arsenic (higher than the accepted level found in water) This was terrifying to me as, like many others, this was my babys first food... and I continued to feed it to him until now. Yes, they are an organic company and they dont spray any pesticides, including arsenic on their food. However, there is arsenic in the soil that has been there for years from others using arsenic as a pesticide years ago and this gets into their products. Now the thing that disgusts me the most is that this organic company that says they care about health, has not recalled any of their rice containing products, including the baby rice. They continue to sell these products to unsuspecting parents who may be compromising their childrens health. Not ...
Commonly found arsenic species in human urine are AsIII (arsenite), AsV (arsenate), MMA (monomethyl arsenic acid), DMA (dimethylarsinic acid) and AB (arsenobetaine). Evidence has shown that these species vary in toxicity and have the potential to be used as biomarkers for human exposure. For human exposure assessments in areas that have naturally occurring arsenic contaminated sources, or those who live or work near contaminated environmental sites where arsenic has been used, it is important to fully understand what species of arsenic residents are being exposed to in order to grasp the risk of arsenic exposure. Since it is difficult to determine direct human exposures, a swine model was used as a surrogate. Urine samples from these studies were analyzed using high-performance liquid chromatography and inductively coupled plasma mass spectrometry (LCICPMS) to quantitate arsenic species. There was a range of 64-74% DMA determined in swine urine samples for all test soils where a range of 60-75% ...
Arsenic is a toxic metalloid which is widely distributed in nature. It is normally present as arsenate under oxic conditions while arsenite is predominant under reducing condition. The major discharges of arsenic in the environment are mainly due to natural sources such as aquifers and anthropogenic sources. It is known that arsenite salts are more toxic than arsenate as it binds with vicinal thiols in pyruvate dehydrogenase while arsenate inhibits the oxidative phosphorylation process. The common mechanisms for arsenic detoxification are uptaken by phosphate transporters, aquaglyceroporins, and active extrusion system and reduced by arsenate reductases via dissimilatory reduction mechanism. Some species of autotrophic and heterotrophic microorganisms use arsenic oxyanions for their regeneration of energy. Certain species of microorganisms are able to use arsenate as their nutrient in respiratory process. Detoxification operons are a common form of arsenic resistance in microorganisms. Hence, the use of
Beyond Pesticides, September 20, 2012) Raising questions about the adequacy of pesticide regulation, historically and ongoing, Consumer Reports published a new study yesterday that finds "worrisome" levels of inorganic arsenic in rice products. Arsenic is a known human carcinogen. This new report follows its earlier one that finds high levels of arsenic in apple and grape juice. The report finds elevated arsenic levels across organic and conventional products, raising serious questions about widespread environmental and soil contamination from past and continuing arsenical pesticide use. Although organic arsenic occurs naturally in the environment, it is synthetic inorganic arsenic that poses the biggest health hazards to humans and animals. So, humans are exposed to two kinds of the carcinogen in air, water, soil, and food sources. But unlike organic arsenic, which is found naturally in the environment, inorganic arsenic is present in our food as a result of pesticide application and animal ...
InChI=1S/C21H24O9/c1-28-16-5-4-11(8-15(16)24)2-3-12-6-13(23)9-14(7-12)29-21-20(27)19(26)18(25)17(10-22)30-21/h2-9,17-27H,10H2,1H3/b3-2+/t17-,18-,19+,20-,21-/m1/s1 ...
1310 Arsenite Schism of Constantinople is officially ended by the reconciliation of the Arsenites to the Josephites, in a ... 1265-1310 Arsenite Schism of Constantinople, beginning when Patr. Arsenius Autorianus excommunicated emperor Michael VIII ... 18(1948), 202-220.) Alexander P. Kahzdan (Ed). "Arsenites." The Oxford Dictionary of Byzantium. Oxford University Press, 2012. ...
Potassium arsenite - KAsO2. *Potassium azide - KN3. *Potassium borate - K2B4O7•4H2O ...
... , also called Schloss Green, is chemically a cupric hydrogen arsenite (also called copper arsenite or acidic ... 3), copper arsenite salt (CuHAsO. 3 and Cu(AsO. 3). 2·3H. 2O)), neutral copper orthoarsenite (3CuO·As. 2O. 3·2H. 2O), copper ... This produced a sodium arsenite solution. Added to a copper sulfate solution, it produced a green precipitate of effectively ... copper arsenite), CuHAsO. 3. It is chemically related to Paris Green. It is a yellowish-green pigment which in the past was ...
vanden Hoven, R.N.; Santini, J.M. (2004). "Arsenite oxidation by the heterotroph Hydrogenophaga sp. str. NT-14: the arsenite ... Arsenate reductase (cytochrome c) (EC 1.20.2.1, arsenite oxidase) is an enzyme with systematic name arsenite:cytochrome c ... This enzyme catalyses the following chemical reaction arsenite + H2O + 2 oxidized cytochrome c ⇌ {\displaystyle \ ... "Arsenite oxidase from Ralstonia sp. 22: characterization of the enzyme and its interaction with soluble cytochromes". J. Biol. ...
Arsenite minerals are much less common. Both the Dana and the Strunz mineral classifications place the arsenates in with the ...
Thus, it dissolves readily in alkaline solutions to give arsenites. It is less soluble in acids, although it will dissolve in ... Bulk arsenic-based compounds sodium arsenite and sodium cacodylate are derived from the trioxide. A variety of applications ...
The generalized reaction catalyzed by members of the ACR3 family is: arsenite or antimonite (in) → arsenite or antimonite (out ... The former yeast protein is present in the plasma membrane and pumps arsenite and antimonite, but not arsenate, tellurite, ... Aaltonen, Emil K. J.; Silow, Maria (2008-04-01). "Transmembrane topology of the Acr3 family arsenite transporter from Bacillus ... The Bacillus protein exports both arsenite and antimonite. The exact transport mechanism has not established. ...
The systematic name of this enzyme class is arsenite:azurin oxidoreductase. This enzyme is also called arsenite oxidase. The ... Ellis PJ, Conrads T, Hille R, Kuhn P (2001). "Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in ... Arsenate reductase (azurin) (EC 1.20.9.1) is an enzyme that catalyzes the chemical reaction arsenite + H2O + 2 azurinox ⇌ {\ ... Anderson GL, Williams J, Hille R (1992). "The purification and characterization of arsenite oxidase from Alcaligenes faecalis, ...
nov., an arsenite-oxidizing bacterium isolated from agricultural soil in Thailand". J. Gen. Appl. Microbiol. 58: 245-51. doi: ... C. terrae has an arsenite-oxidizing ability. http://www.bacterio.cict.fr/c/comamonas.html http://www.straininfo.net/strains/ ...
... can metabolize arsenate and arsenite. A.C. Parte. "Marinobacter". bacterio.net. Retrieved 2016-08- ... nov., an arsenate-respiring and arsenite-oxidizing bacterium isolated from hydrothermal sediment". International Journal of ...
MM-1 isolated from a soil and identification of arsenite oxidase gene". Journal of Hazardous Materials. 262: 997-1003. doi: ... Bahar, Md Mezbaul; Megharaj, Mallavarapu; Naidu, Ravi (2013-11-15). "Kinetics of arsenite oxidation by Variovorax sp. ...
nov., an arsenite-oxidizing bacterium from Thai soil". Antonie van Leeuwenhoek. 106 (6): 1239-46. doi:10.1007/s10482-014-0294-1 ... Flavobacterium arsenitoxidans is a Gram-negative, arsenite-oxidizing, non-spore-forming, rod-shaped and aerobic bacterium from ...
nov., an arsenite-resistant bacterium isolated from soil". International Journal of Systematic and Evolutionary Microbiology. ...
nov., an arsenite-resistant bacterium isolated from soil". International Journal of Systematic and Evolutionary Microbiology. ... Xenophilus arseniciresistens is a Gram-negative, aerobic, arsenite-resistant and motile bacterium from the genus Xenophilus ...
Arsenite or Antimonite (out). The overall reaction catalyzed by ArsB-ArsA is: Arsenite or Antimonite (in) + ATP ⇌ Arsenite or ... Arsenic toxicity Arsenite Antimonite Ion transporter Ion transporter superfamily ARC3 family Arsenite-Antimonite efflux ... Arsenite resistance (Ars) efflux pumps of bacteria may consist of two proteins, ArsB (TC# 2.A.45.1.1; the integral membrane ... These pumps actively expel both arsenite and antimonite. Homologues of ArsB are found in Gram-negative and Gram-positive ...
... arsenite, hexachlorobenzene, and nickel compounds. Many teratogens exert specific effects on the fetus by epigenetic mechanisms ...
Induced by oxidative stress generated by arsenite treatment. Binding of nitric oxide (NO) to the heme iron in the N-terminal ...
... is a species of arsenite-oxidizing bacterium. Salmassi, Tina M.; Venkateswaren, Kasthuri; Satomi, ... Masataka; Newman, Dianne K.; Hering, Janet G. (2002). "Oxidation of Arsenite by Agrobacterium albertimagni , AOL15, sp. nov., ...
arsenite + photonslight energy → (AsO3−. 4). arsenate + COcarbon. monoxide(used to build other compounds in subsequent ... Other processes substitute other compounds (such as arsenite) for water in the electron-supply role; for example some microbes ... use sunlight to oxidize arsenite to arsenate:[15] The equation for this reaction is: CO2carbon. dioxide + (AsO3−. 3). ...
Arsenite is found to bond to sulfur compounds in the protein of degraded hair, which is a key component in bezoars. A famous ... The toxic compounds in arsenic are arsenate and arsenite. Each is acted upon differently, but effectively, by bezoar stones. ...
Potassium arsenite, colorless solid, toxic. *Potassium bromate, colorless, oxidizing agent, used in flour ...
Arsenite (As(+3)), has a pKa of 9.3, and occurs at a neutral or acidic pH as As(OH)3. Arsenite in water can be thought of as an ... On the other hand, arsenite is more common in anaerobic environments, more mobile, and more toxic than arsenate. Arsenite is 25 ... Living cells (microbial or human) are generally exposed to arsenic as arsenate or arsenite. Arsenate (As(+5)) has a pKa of 7.0 ... In microbial biofilms growing on the rock surfaces of anoxic brine pools fed by hot springs containing arsenite and sulfide at ...
The presence or absence of P does not affect the uptake of arsenite, which translocates more easily than arsenate. C. Tu, L.Q. ... arsenite as a noncompetitive inhibitor). Priel 1995. Gurta et al. 1994 L.E. Bennetta; J.L. Burkheada; K.L. Halea; N. Terry; M. ... the plants then absorbs 10 times more arsenate than arsenite. If on the other hand the P supply is increased, As uptake ...
... has the ability to oxidize arsenite to arsenate. The complete genome of A. arsenitoxydans has been ...
This two-subunit enzyme produces resistance to arsenite and antimonite. Arsenate, however, must first be reduced to arsenite ... It is required for the detoxification of arsenate, arsenite, and antimonite. This system transports arsenite and antimonite out ... thus increasing its ATPase activity at lower concentrations of arsenite and enhancing the rate of arsenite extrusion. Carlin A ... ArsC is an approximately 150-residue arsenate reductase that uses reduced glutathione (GSH) to convert arsenate to arsenite ...
Sodium arsenite exposure at concentration >5 M may induce embryotoxic and Published by sunolmolecular UnderCCK Receptors on ... 1 Sodium arsenite treatment of mouse ESC induced G2/M arrest followed by apoptotic cell death. a Phase contrast microscopy (40 ... Results Sodium arsenite treatment modulates signaling pathways that control self-renewal and survival of mouse ESC In mouse ESC ... During embryogenesis, salt arsenite, which is normally known as transplacental carcinogen [23], might have an effect on growth ...
Some arsenite salts can be prepared from an aqueous solution of As2O3. Examples of these are the meta-arsenite salts and at low ... A number of different arsenite anions are known: AsO3− 3 ortho-arsenite, an ion of arsenous acid, with a pyramidal shape [AsO− ... Well known examples of arsenites include sodium meta-arsenite which contains a polymeric linear anion, [AsO− 2] n, and silver ... 2] n meta-arsenite, a polymeric chain anion. As 2O4− 5 pyro-arsenite, O2As-O-AsO2 As 3O5− 7 a polyarsenite, [O2As-O-As(O)-O- ...
... potassium meta-arsenite (KAsO2) and potassium ortho-arsenite (K3AsO3). It is composed of arsenite ions (AsO33− or AsO2−) with ... Potassium arsenite is still, however, used as a rodenticide. The two unique forms of potassium arsenite can be attributed to ... Potassium arsenite also reacts with acids to yield toxic arsine gas. Aqueous potassium arsenite, more commonly known as ... Additionally, arsenite containing compounds have also been labeled carcinogens. The carcinogenicity of potassium arsenite ...
This is unique source of information on the chemicals manufactured and imported in Europe. It covers their hazardous properties, classification and labelling, and information on how to use them safely.. REACH. ...
Triethyl arsenite. Regulatory process names 2 IUPAC names 1 Other identifiers 1 ...
This page contains information on the chemical Copper arsenite including: 17 synonyms/identifiers; U.S. Code of Federal ... Copper arsenite*Copper arsenite [UN1586] [Poison]*Copper arsonate*Copper orthoarsenite*Copper(II) arsenite*CUPRIC ARSENITE* ... Copper arsenite. Identifications. *Caswell Number: 233*Synonyms/Related:*Acid copper arsenite*Air-flo Green*Arsenious acid ( ... Copper arsenite. No. No. Related Resources. *USDOT Hazardous Materials Table 49 CFR 172.101. An online version of the USDOTs ...
This page contains information on the chemical Strontium arsenite including: 3 synonyms/identifiers; U.S. Code of Federal ... Strontium arsenite*Strontium arsenite (Sr(AsO2) 2) 49 CFR 172.101 - Hazardous Materials Table. Hazardous materials description ... Strontium arsenite (EnvironmentalChemistry.com),/a,- This page contains information on the chemical Strontium arsenite ... Strontium arsenite. 6.1. UN1691. II. 6.1. IB8, IP2, IP4, T3, TP33. 153. 212. 242. Forbid. Forbid. A. ...
... Zhibin Zhang,1,2 Yulin Sun,1 Cuizhen Sun,1 Ning Wang,1 ... Adsorption of arsenite using six submerged plants (Mimulicalyx rosulatus, Potamogeton maackianus, Hydrilla, Watermifoil, Pteris ...
Article Removal of arsenite from water by soil biotechnology. In this paper we present oxidation of As(III) to As(V) by ... No comments were found for Removal of arsenite from water by soil biotechnology. Be the first to comment! ... Keywords: arsenic oxidation, SBT, soil biotechnology, As (III) adsorption, hydrous ferric oxide, arsenite, bioremediation, ...
... low-level sodium arsenite induced apoptosis via caspase-3 in INS-1 cells. Knockdown of ASK1 alleviated sodium arsenite-induced ... Low-level sodium arsenite induces apoptosis through inhibiting TrxR activity in pancreatic β-cells.. Yao XF1, Zheng BL1, Bai J1 ... After treatment with low-level (0.25-1μM) sodium arsenite for 96h, the thioredoxin reductase (TrxR) activity was decreased ... In our previous study, we reported that sodium arsenite induced ROS-dependent apoptosis through lysosomal-mitochondrial pathway ...
J. Pi, Y. He, C. Bortner et al., "Low level, long-term inorganic arsenite exposure causes generalized resistance to apoptosis ... Culture media, FBS, and supplements were purchased from Invitrogen (Carlsbad, CA, USA). Sodium arsenite and curcumin were ... Our previous studies reveal that oxidative stress occurs in response to inorganic arsenite (iAs3+) exposure [5-8], which may ... "Nrf2 protects human bladder urothelial cells from arsenite and monomethylarsonous acid toxicity," Toxicology and Applied ...
Sodium arsenite was used by taxidermists in arsenical soaps for cleaning and sterilizing hides. It was also used as an ... Retrieved from "http://cameo.mfa.org/index.php?title=Sodium_arsenite&oldid=40138" ...
By stimulating human endothelial cells (SVEC4-10) with arsenite, we found that arsenite modulated the integrity of actin ... Further, we found arsenite increased the cell motility, indicating that structural ch ... Arsenite is a human carcinogen, however, the molecular mechanisms involved are poorly understood. ... By stimulating human endothelial cells (SVEC4-10) with arsenite, we found that arsenite modulated the integrity of actin ...
This arsenite exposure resulted in HIF-1A accumulation and increased glycolysis, similar to the physiologic response to hypoxia ... Constant exposure to 1 µM arsenite (As) resulted in the early loss of anchorage-dependent growth, measured by soft agar colony ... and it has been found to accumulate during arsenite exposure under oxygen-replete conditions. We modeled arsenic-exposed human ...
Sodium arsenite Ellagic acid Hematological parameters Cardioprotection Rat This is a preview of subscription content, log in to ... Group 2 was orally treated with sodium arsenite (SA, 10 mg/kg) for 21 days. Group 3 was orally treated with EA (30 mg/kg) for ... Chen, C., Jiang, X., Hu, Y., & Zhang, Z. (2013). The protective role of resveratrol in the sodium arsenite-induced oxidative ... Adil, M., Kandhare, A. D., Visnagri, A., & Bodhankar, S. L. (2015). Naringin ameliorates sodium arsenite-induced renal and ...
An arsenite-resistant strain of Leishmania donovani was generated in vitro by sequential exposure to higher concentrations of ... Arsenite-resistant Leishmania donovani promastigotes express an enhanced membrane P-type adenosine triphosphatase activity that ... sodium m-arsenite. The resistant strain displayed a low level of cross-resistance to structurally unrelated drugs such as ...
Arsenite oxidation. Bosea sp. WAO is able to grow under chemolithoautotrophic conditions with arsenite in addition to growing ... The arsenite oxidase genes (aroAB) in novel chemoautotrophic arsenite oxidizers. Biochem Biophys Res Commun. 2007;354:662-7. ... Operon structure for arsenite oxidation viewed 5′-3′ direction on the plus strand. The gene order is the same in both Bosea sp ... WAO, only the large subunit gene for the arsenite oxidation pathway aioA (EF015463) was amplified by traditional PCR [1, 16]. ...
The present study aimed at comparing the toxic effect of DMMTA(V) with that of inorganic arsenite (iAs(III)) on cell viability ... Arsenites / pharmacokinetics, toxicity. Cacodylic Acid / analogs & derivatives, pharmacokinetics, toxicity. Carcinogenicity ... 0/Aquaporins; 0/Arsenicals; 0/Arsenites; 0/Environmental Pollutants; 0/Reactive Oxygen Species; 0/dimethylmonothioarsinic acid ... and because the resistance of cells to arsenite can be influenced by lower arsenic uptake due to lower expression of aquaporin ...
... arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol ... transporter activity protein binding ATP binding nucleus nucleolus cytoplasm endoplasmic reticulum membrane transport arsenite ... transporter activity protein binding ATP binding nucleus nucleolus cytoplasm endoplasmic reticulum membrane transport arsenite ...
Arsenite efflux transporter ArsBImported. ,p>Information which has been imported from another database using automatic ... tr,Q735E4,Q735E4_BACC1 Arsenite efflux transporter ArsB OS=Bacillus cereus (strain ATCC 10987 / NRS 248) OX=222523 GN=BCE_3208 ...
PubMed journal article Arsenite exposure accelerates aging process regulated by the transcription factor DAF-16/FOXO in ... Arsenite exposure accelerates aging process regulated by the transcription factor DAF-16/FOXO in Caenorhabditis elegans.. ... TY - JOUR T1 - Arsenite exposure accelerates aging process regulated by the transcription factor DAF-16/FOXO in Caenorhabditis ... In addition, arsenite exposure (100 μM) caused significant changes of age-dependent biomarkers, including a decrease of ...
The three ars genes conferred arsenite resistance in E. coli and arsenite as well as arsenate resistance in Bacillus subtilis. ... The arsenate, arsenite, and antimonite resistance was shown to be inducible by all three heavy metal ions. Inactivation of the ... The arsenate, arsenite, and antimonite resistance region of the Staphylococcus xylosus plasmid pSX267 was subcloned in ... The inactivation of arsR also resulted in reduction of resistance to arsenite and antimonite, while arsenate resistance was ...
An extracellular pH-driven targeted multifunctional manganese arsenite delivery system for tumor imaging and therapy ... An extracellular pH-driven targeted multifunctional manganese arsenite delivery system for tumor imaging and therapy K. Zhang, ...
Long-term low-dose exposure of human urothelial cells to sodium arsenite activates lipocalin-2 via promoter hypomethylation.. [ ... We previously reported that the sustained exposure of human urothelial cells (HUCs) to low-dose sodium arsenite induces changes ...
This strain could grow with arsenite (AsIII) as an energy source, CO2-HCO3- as a carbon source and oxygen as the electron ... This strain could grow with arsenite (AsIII) as an energy source, CO2-HCO3- as a carbon source and oxygen as the electron ... A new facultative chemolithoautotrophic arsenite-oxidizing bacterium TCC9-4 was isolated from a hot spring microbial mat in ... A new facultative chemolithoautotrophic arsenite-oxidizing bacterium TCC9-4 was isolated from a hot spring microbial mat in ...
  • Mesoporous pellet adsorbent developed from mixing at an appropriate ratio of natural clay, iron oxide, iron powder, and rice bran was used to investigate the optimization process of batch adsorption parameters for treating aqueous solution coexisting with arsenate and arsenite. (gjesm.net)
  • The adsorption efficiency of coexisting arsenate and arsenite solution onto the new developed adsorbent was over 99% under the optimized experimental condition. (gjesm.net)
  • Since the thioredoxin (Trx) system is the key antioxidant factor in mammalian cells, we investigate whether the inhibition of Trx system contributes to sodium arsenite-induced apoptosis in this study. (nih.gov)
  • The most prominent target of enzymatic inhibition by arsenite is IkB kinase-beta. (nih.gov)
  • Markers indicative of apoptosis (phosphatidylserine externalization, PARP cleavage, and sensitivity to caspase inhibition) were uniformly negative in arsenite exposed cells. (elsevier.com)
  • 2. Experiments with kidney and liver minces and with yeast have shown that indeed the inhibition caused by urethan and arsenite increases with increasing rate of respiration or fermentation. (uu.nl)
  • 4. The dependence of inhibition upon the turnover number of the catabolic enzyme system in tumours is discussed in relation to other possible causes of the effect of urethan and arsenite on malignant growth. (uu.nl)
  • Northern hybridization and western blot analysis revealed that exposure of A6 cells to 1-10 μM sodium arsenite at a mild heat shock temperature of 30˚C enhanced hsp30 and hsp70 gene expression to a much greater extent than found with either stress individually. (uwaterloo.ca)
  • In cells treated simultaneously with 10 μM sodium arsenite and different heat shock temperatures, enhanced accumulation of HSP30 and HSP70 protein was first detected at 26˚C with larger responses at 28 and 30 ˚C. HSF1 activity was involved in combined stress-induced hsp gene expression since the HSF1 activation inhibitor, KNK437, inhibited HSP30 and HSP70 accumulation. (uwaterloo.ca)
  • Western blotting with anti-AoxB antibodies and quantitative RT-PCR experiments allowed us to demonstrate that the rpoN and dnaJ gene products are involved in the control of arsenite oxidase gene expression. (biomedcentral.com)
  • In this study we utilized genome-wide gene expression analysis and functional assays to characterize arsenic-induced effects in seven LCLs that were exposed to an environmentally relevant, minimally cytotoxic, concentration of arsenite (0.75 μM) over an eight-day time course. (elsevier.com)
  • This construct was used to investigate the role of mitogen-activated protein (MAP) kinases in arsenite-mediated heme oxygenase-1 gene expression. (umassmed.edu)
  • Involvement of an AP-1 site in arsenite induction of heme oxygenase-1 gene expression was studied. (umassmed.edu)
  • Our previous study demonstrated an elevated arsenic level, ultra-structure changes and reduced NR2A gene expression in hippocampus, and impaired spatial learning in arsenite-exposed rats. (epa.gov)
  • In our previous study, we reported that sodium arsenite induced ROS-dependent apoptosis through lysosomal-mitochondrial pathway in pancreatic β-cells. (nih.gov)
  • Finally, low-level sodium arsenite induced apoptosis via caspase-3 in INS-1 cells. (nih.gov)
  • Knockdown of ASK1 alleviated sodium arsenite-induced apoptosis. (nih.gov)
  • Taken together, these data suggest that a possible mechanism of arsenite-induced apoptosis is the accumulation of ubiquitinated proteins and impairment of the protein degradative pathway. (naver.com)
  • The primary speculation, which acquired been attended to in the present research, was that salt arsenite might focus on many signaling paths in ESC straight, controlling self-renewal and marketing apoptosis. (sunolmolecular.com)
  • a Phase contrast microscopy (40 magnification) of mouse ESC (cultured as adherent cells) in the absence and in the presence of 4 M sodium arsenite, … FACS assays of PI-stained nuclei revealed strong dose-dependent changes in cell cycle regulation for stem cells that resulted in G2/M arrest 24 h after arsenic treatment followed by pronounced apoptosis 48 h after treatment (Fig. 1dCf). (sunolmolecular.com)
  • PI-3K/Akt pathway-dependent cyclin D1 expression is responsible for arsenite-induced human keratinocyte transformation. (nih.gov)
  • 2006). To determine the potential involvement of the PI-3K pathway in arsenite-induced HaCat cell transformation, we tested the PI-3K activity in arsenite-exposed HaCat cells. (nih.gov)
  • We provide a novel scenario of the proapoptotic role of IkappaB kinase beta (IKKbeta)-NF-kappaB, which can act as the activator of the JNK pathway through the induction of GADD45alpha for triggering MKK4/JNK activation, in response to the stimulation of arsenite, a cancer therapeutic reagent. (exeter.ac.uk)
  • We D-(+)-Xylose supplier also elucidate a possible mechanism of the resistance to apoptotic death induced by sodium arsenite in neural stem/precursor cells based on the IL6CStat3 pathway. (sunolmolecular.com)
  • The high-osmolarity glycerol (HOG) pathway is also activated by arsenite, and the hog1 mutant is highly sensitive to arsenite, partly due to increased arsenite influx into hog1 cells. (agriculture-xprt.com)
  • By stimulating human endothelial cells (SVEC4-10) with arsenite, we found that arsenite modulated the integrity of actin filaments, and stimulated the formation of cell motility organelle structures within 5 minutes. (cdc.gov)
  • In the present study, we found that arsenite is able to induce HIF-1α protein accumulation in both mouse epidermal Cl41 cells and mouse embryonic fibroblasts (MEFs). (aacrjournals.org)
  • However, the exact mechanisms of arsenite-induced human skin carcinogenesis remain to be defined. (nih.gov)
  • Genetic basis of arsenite and cadmium tolerance in Saccharomyces cerevisiae. (chalmers.se)
  • RESULTS: To gain insight into metal action and cellular tolerance mechanisms, we carried out genome-wide screening of the Saccharomyces cerevisiae haploid and homozygous diploid deletion mutant collections and scored for reduced growth in the presence of arsenite or cadmium. (chalmers.se)
  • Arsenite-specific defence functions were related to cell cycle regulation, lipid and fatty acid metabolism, mitochondrial biogenesis, and the cytoskeleton whereas cadmium-specific defence functions were mainly related to sugar/carbohydrate metabolism, and metal-ion homeostasis and transport. (chalmers.se)
  • Molecular evidence indicated that the cytoskeleton is targeted by arsenite and that phosphorylation of the Snf1p kinase is required for cadmium tolerance. (chalmers.se)
  • Arsenite and Cadmium Activate MAPK/ERK via Membrane Estrogen Receptors" by Mary Huff et al. (bellarmine.edu)
  • Here, we examined the effect of cadmium chloride (CdCl 2 ) and sodium arsenite (NaAsO 2 ) on the proliferation of human lung adenocarcinoma cell lines. (bellarmine.edu)
  • In LMH cells, sodium arsenite, cadmium, and heat shock, but not heme, induced activity of the MAP kinases extracellular-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. (umassmed.edu)
  • Below are the list of possible Arsenite resistance protein products. (mybiosource.com)
  • GM847 lung fibroblasts and HaCaT keratinocytes were exposed to sodium arsenite, As(III), and mRNA, protein levels and BER activity were assessed. (edu.au)
  • Arsenite stimulated an increase in protein ubiquitination in GCS-2 cells while the wild-type cells were unaffected. (naver.com)
  • Moreover, the Sic1 protein was stabilized in arsenite-treated hog1 cells. (agriculture-xprt.com)
  • Decreased protein expression of NR2A, PSD-95 and p-CaMKII α in the hippocampus of arsenite-exposed rats was observed, while the expression of SynGAP, a negative regulator of Ras-MAPK activity, was increased when compared with the controls. (epa.gov)
  • The present study aimed at identifying curcumin as a potent activator of nuclear factor erythroid 2-related factor 2 (NRF2) and demonstrating its protective effect against inorganic arsenite- (iAs 3+ -) induced cytotoxicity in human keratinocytes. (hindawi.com)
  • The present study aimed at comparing the toxic effect of DMMTA(V) with that of inorganic arsenite (iAs(III)) on cell viability, uptake efficiency and production of reactive oxygen species (ROS) toward human bladder cancer EJ-1 cells. (biomedsearch.com)
  • IUPAC have recommended that arsenite compounds are to be named as arsenate(III), for example ortho-arsenatite is called trioxidoarsenate(III). (wikipedia.org)
  • Affinity absorption assays with GST-PAK-PBD demonstrated that arsenite activated small GTPase CDC42 and Rac. (cdc.gov)
  • Ameliorative role of conjugated linolenic acid isomers against oxidative DNA damage induced by sodium arsenite in rat model. (springer.com)
  • Arsenate is salt or ester of arsenic acid having a negative ion of AsO 4 3- , arsenite is a salt or ester of arsenious acid having a negative ion of AsO 4 3- derived from aqueous solutions of As 4 O 6 and arsenide is a negative, trivalent binary arsenic compound such as H 3 As or GaAs. (chemicalland21.com)
  • It is then distributed throughout the body, where it is reduced into arsenite if necessary, then methylated into monomethylarsenic (MMA) and dimethylarsenic acid (DMA) by arsenite methyltransferase. (t3db.ca)
  • A total of 552 Campylobacter isolates (281 Campylobacter jejuni and 271 Campylobacter coli ) isolated from retail meat samples were subjected to arsenic resistance profiling using the following arsenic compounds: arsanilic acid (4-2,048 μg/mL), roxarsone (4-2048 μg/mL), arsenate (16-8,192 μg/mL) and arsenite (4-2,048 μg/mL). (mdpi.com)
  • Most of the 552 Campylobacter isolates were able to survive at higher concentrations of arsanilic acid (512-2,048 μg/mL), roxarsone (512-2,048 μg/mL), and arsenate (128-1,024 μg/mL), but at lower concentrations for arsenite (4-16 μg/mL). (mdpi.com)
  • Resistance to arsenite (As(III)) by cells is generally accomplished by arsenite efflux permeases from Acr3 or ArsB unrelated families. (ac.be)
  • We investigated the cytotoxicity of sodium arsenite in LCL 18564 using a set of complementary markers for cell death pathways. (elsevier.com)
  • We possess additional recommended in the present research that sodium arsenite exposure might target the embryonic neurogenesis in mice via interference and interaction with cell signaling pathways in mouse neural stem/precursor cells. (sunolmolecular.com)
  • Results Sodium arsenite treatment modulates signaling pathways that control self-renewal and survival of mouse ESC In mouse ESC exposed to graded doses of sodium arsenite (1C6 M, 24C48 h), there was a dramatic dose-dependent reduction in cell survival as shown in Fig. 1. (sunolmolecular.com)
  • Elevated levels of intracellular arsenite and 'cross talk' between the HOG and pheromone response pathways, observed in arsenite-treated hog1 cells, prolonged the G1 delay but did not cause a persistent G1 arrest. (agriculture-xprt.com)
  • The three ars genes conferred arsenite resistance in E. coli and arsenite as well as arsenate resistance in Bacillus subtilis. (asm.org)
  • As(III) and antimonite (Sb(III)) resistance and accumulation studies using E. coli or C. glutamicum arsenite permease mutants clearly show that CgAcr3-1 is specific for As(III). (ac.be)
  • Filtration is only effective if arsenic is present as particulates, if the arsenite is in solution it passes though the filtration membrane. (wikipedia.org)
  • Arsenite-resistant Leishmania donovani promastigotes express an enhanced membrane P-type adenosine triphosphatase activity that is sensitive to ver. (nih.gov)
  • Arsenite is a human carcinogen, however, the molecular mechanisms involved are poorly understood. (cdc.gov)
  • In this study, we investigated the effects and the underlying mechanisms of chronic arsenite exposure on the aging process in Caenorhabditis elegans. (unboundmedicine.com)
  • The purpose of this study was to examine mechanisms of genotoxicity induced by environmentally relevant doses of arsenite (As(+3)) in mouse thymus cells. (sigmaaldrich.com)
  • Although hypoxia-inducible factor-1α (HIF-1α) has been reported to be activated by arsenite both in vivo and in vitro, the detailed molecular mechanisms leading to HIF-1α expression and activation due to arsenite exposure have not been well understood. (aacrjournals.org)
  • Nevertheless, the molecular mechanisms involved in the control of arsenite oxidase expression remain largely unknown. (biomedcentral.com)
  • This study analyzes how the indigenous arsenite-oxidizing bacteria combat As stress by various biophysical alterations and self-adaptation mechanisms. (iisc.ac.in)
  • arsenite blocks an equal part of the sensitive enzyme(s), by which a measurable effect of a small concentration of these inhibitors on the catabolism will only be obtained in case of maximal turnover rate. (uu.nl)
  • Some species of bacteria obtain their energy by oxidizing various fuels while reducing arsenates to form arsenites. (wikipedia.org)
  • In 2008, bacteria were discovered that employ a version of photosynthesis with arsenites as electron donors, producing arsenates (just like ordinary photosynthesis uses water as electron donor, producing molecular oxygen). (wikipedia.org)
  • a) Arsenic sulfide, arsenates, or arsenites. (cornell.edu)
  • Interestingly, electron microscopy, acidic vesicle fluorescence, and expression of LC3 in LCL 18564 identified autophagy as an arsenite-induced process that was associated with cytotoxicity. (elsevier.com)
  • Autophagy, a cellular programmed response that is associated with both cellular stress adaptation as well as cell death appears to be the predominant process in LCL cytotoxicity induced by arsenite. (elsevier.com)
  • It is unclear, however, whether LCL autophagy is an effector mechanism of arsenite cytotoxicity or alternatively a cellular compensatory mechanism. (elsevier.com)
  • Cytotoxicity and genotoxicity has beenevaluated by exposure of IEC-6 cells to arsenate (5mM)and arsenite (1.5mM)at low concentrations. (manipal.edu)
  • Efflux permease CgAcr3-1 of Corynebacterium glutamicum is an arsenite-specific antiporter. (ac.be)
  • Therefore, we propose that CgAcr3-1 is an antiporter that catalyzes arsenite-proton exchange with residues Cys129 and Glu305 involved in efflux. (ac.be)
  • A new facultative chemolithoautotrophic arsenite (As III )-oxidizing bacterium TCC9-4 was isolated from a hot spring microbial mat in Tengchong of Yunnan, China. (frontiersin.org)
  • Microbial arsenite [As(III)] chemolithotrophy was first reported by Ilyaletdinov and Abdrashitova ( 18 ) and then more recently by Santini et al. (asm.org)
  • Because the cellular uptake of iAs(III) is mediated by aquaporin proteins, and because the resistance of cells to arsenite can be influenced by lower arsenic uptake due to lower expression of aquaporin proteins (AQP 3, 7 and 9), the expression of several members of the aquaporin family was also examined. (biomedsearch.com)
  • Expression and regulation of the antimonite, arsenite, and arsenate resistance operon of Staphylococcus xylosus plasmid pSX267. (asm.org)
  • The arsenate, arsenite, and antimonite resistance region of the Staphylococcus xylosus plasmid pSX267 was subcloned in Staphylococcus carnosus. (asm.org)
  • The arsenate, arsenite, and antimonite resistance was shown to be inducible by all three heavy metal ions. (asm.org)
  • The inactivation of arsR also resulted in reduction of resistance to arsenite and antimonite, while arsenate resistance was unaffected. (asm.org)
  • Seems to confer resistance to arsenite by allowing cells to extrude this compound. (mybiosource.com)
  • To identify additional molecules that mediate SKN-1 signaling to the NMJ, we performed a candidate screen for suppressors of aldicarb resistance caused by acute treatment with the SKN-1 activator arsenite. (genetics.org)
  • In the present study, I examined the effect of concurrent low doses of sodium arsenite and mild heat shock temperatures on the expression of hsp30 and hsp70 genes in Xenopus laevis A6 kidney epithelial cells. (uwaterloo.ca)
  • When cells were transfected with a plasmid expressing the p65 subunit of NF-κB, the inhibitory effect of sodium arsenite on NF-κB activity and IL-6 production was blunted. (portlandpress.com)
  • The enzyme from the distantly related heterotroph, Alcaligenes faecalis, which is thought to oxidise arsenite for detoxification, consists of a large α subunit (AioA) with bis-molybdopterin guanine dinucleotide at its active site and a 3Fe-4S cluster, and a small β subunit (AioB) which contains a Rieske 2Fe-2S cluster. (wordpress.com)