Arsenite transporting atpases. Medical search

Inorganic salts or organic esters of arsenious acid.

Inorganic compounds that contain sodium as an integral part of the molecule.

Efflux pumps that use the energy of ATP hydrolysis to pump arsenite across a membrane. They are primarily found in prokaryotic organisms, where they play a role in protection against excess intracellular levels of arsenite ions.

A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.

Inorganic or organic salts and esters of arsenic acid.

A shiny gray element with atomic symbol As, atomic number 33, and atomic weight 75. It occurs throughout the universe, mostly in the form of metallic arsenides. Most forms are toxic. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), arsenic and certain arsenic compounds have been listed as known carcinogens. (From Merck Index, 11th ed)

Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.

Cation-transporting proteins that utilize the energy of ATP hydrolysis for the transport of CALCIUM. They differ from CALCIUM CHANNELS which allow calcium to pass through a membrane without the use of energy.

Proton-translocating ATPases that are involved in acidification of a variety of intracellular compartments.

A general class of integral membrane proteins that transport ions across a membrane against an electrochemical gradient.

A metallic element that has the atomic symbol Sb, atomic number 51, and atomic weight 121.75. It is used as a metal alloy and as medicinal and poisonous salts. It is toxic and an irritant to the skin and the mucous membranes.

An agent that causes the production of physical defects in the developing embryo.

Oxidoreductases that specifically reduce arsenate ion to arsenite ion. Reduction of arsenate is a critical step for its biotransformation into a form that can be transported by ARSENITE TRANSPORTING ATPASES or complexed by specific sulfhydryl-containing proteins for the purpose of detoxification (METABOLIC DETOXIFICATION, DRUG). Arsenate reductases require reducing equivalents such as GLUTAREDOXIN or AZURIN.

Inorganic or organic compounds that contain arsenic.

Calcium-transporting ATPases found on the PLASMA MEMBRANE that catalyze the active transport of CALCIUM from the CYTOPLASM into the extracellular space. They play a role in maintaining a CALCIUM gradient across plasma membrane.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.

A subgroup of aquaporins that transport WATER; GLYCEROL; and other small solutes across CELL MEMBRANES.

A schistosomicide possibly useful against other parasites. It has irritant emetic properties and may cause lethal cardiac toxicity among other adverse effects.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Membrane proteins whose primary function is to facilitate the transport of positively charged molecules (cations) across a biological membrane.

The type species of gram negative bacteria in the genus ALCALIGENES, found in soil. It is non-pathogenic, non-pigmented, and used for the production of amino acids.

A large group of bacteria including those which oxidize ammonia or nitrite, metabolize sulfur and sulfur compounds, or deposit iron and/or manganese oxides.

A family in the order Chromatiales, class GAMMAPROTEOBACTERIA. These are haloalkaliphilic, phototrophic bacteria that deposit elemental sulfur outside their cells.

An enzyme that catalyzes the active transport system of sodium and potassium ions across the cell wall. Sodium and potassium ions are closely coupled with membrane ATPase which undergoes phosphorylation and dephosphorylation, thereby providing energy for transport of these ions against concentration gradients.

A species of extremely thermophilic, sulfur-reducing archaea. It grows at a maximum temperature of 95 degrees C. in marine or deep-sea geothermal areas.

Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.

The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

Integral membrane proteins that transport protons across a membrane. This transport can be linked to the hydrolysis of ADENOSINE TRIPHOSPHATE. What is referred to as proton pump inhibitors frequently is about POTASSIUM HYDROGEN ATPASE.

A plant genus of the family PTERIDACEAE. Members contain entkaurane DITERPENES. The name is similar to bracken fern (PTERIDIUM).

Calcium-transporting ATPases that catalyze the active transport of CALCIUM into the SARCOPLASMIC RETICULUM vesicles from the CYTOPLASM. They are primarily found in MUSCLE CELLS and play a role in the relaxation of MUSCLES.

A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.

Disorders associated with acute or chronic exposure to compounds containing ARSENIC (ARSENICALS) which may be fatal. Acute oral ingestion is associated with gastrointestinal symptoms and an encephalopathy which may manifest as SEIZURES, mental status changes, and COMA. Chronic exposure is associated with mucosal irritation, desquamating rash, myalgias, peripheral neuropathy, and white transverse (Mees) lines in the fingernails. (Adams et al., Principles of Neurology, 6th ed, p1212)

A cadmium halide in the form of colorless crystals, soluble in water, methanol, and ethanol. It is used in photography, in dyeing, and calico printing, and as a solution to precipitate sulfides. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)

The rate dynamics in chemical or physical systems.

The process of cleaving a chemical compound by the addition of a molecule of water.

A genus in the family BURKHOLDERIACEAE, comprised of many species. They are associated with a variety of infections including MENINGITIS; PERITONITIS; and URINARY TRACT INFECTIONS.

The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.

Oxyvanadium ions in various states of oxidation. They act primarily as ion transport inhibitors due to their inhibition of Na(+)-, K(+)-, and Ca(+)-ATPase transport systems. They also have insulin-like action, positive inotropic action on cardiac ventricular muscle, and other metabolic effects.

A family of gram-negative bacteria in the class BETAPROTEOBACTERIA. There are at least eight genera.

A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.

Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.

Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

Behaviors associated with the ingesting of alcoholic beverages, including social drinking.

A ubiquitous family of proteins that transport PHOSPHOLIPIDS such as PHOSPHATIDYLINOSITOL and PHOSPHATIDYLCHOLINE between membranes. They play an important role in phospholipid metabolism during vesicular transport and SIGNAL TRANSDUCTION.

A primary, chronic disease with genetic, psychosocial, and environmental factors influencing its development and manifestations. The disease is often progressive and fatal. It is characterized by impaired control over drinking, preoccupation with the drug alcohol, use of alcohol despite adverse consequences, and distortions in thinking, most notably denial. Each of these symptoms may be continuous or periodic. (Morse & Flavin for the Joint Commission of the National Council on Alcoholism and Drug Dependence and the American Society of Addiction Medicine to Study the Definition and Criteria for the Diagnosis of Alcoholism: in JAMA 1992;268:1012-4)

A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES.

Acyltransferases in the inner mitochondrial membrane that catalyze the reversible transfer of acyl groups from acyl-CoA to L-carnitine and thereby mediate the transport of activated fatty acids through that membrane. EC 2.3.1.

Drinkable liquids containing ETHANOL.

Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.

Expression cloning for arsenite-resistance resulted in isolation of tumor-suppressor fau cDNA: possible involvement of the ubiquitin system in arsenic carcinogenesis. (1/104)

Arsenic is a human carcinogen whose mechanism of action is unknown. Previously, this laboratory demonstrated that arsenite acts as a comutagen by interfering with DNA repair, although a specific DNA repair enzyme sensitive to arsenite has not been identified. A number of stable arsenite-sensitive and arsenite-resistant sublines of Chinese hamster V79 cells have now been isolated. In order to gain understanding of possible targets for arsenite's action, one arsenite-resistant subline, As/R28A, was chosen as a donor for a cDNA expression library. The library from arsenite-induced As/R28A cells was transfected into arsenite-sensitive As/S5 cells, and transfectants were selected for arsenite-resistance. Two cDNAs, asr1 and asr2, which confer arsenite resistance to arsenite-hypersensitive As/S5 cells as well as to wild-type cells, were isolated. asr1 shows almost complete homology with the rat fau gene, a tumor suppressor gene which contains a ubiquitin-like region fused to S30 ribosomal protein. Arsenite was previously shown to inhibit ubiquitin-dependent proteolysis. These results suggest that the tumor suppressor fau gene product or some other aspect of the ubiquitin system may be a target for arsenic toxicity and that disruption of the ubiquitin system may contribute to the genotoxicity and carcinogenicity of arsenite. (+info)

Asp45 is a Mg2+ ligand in the ArsA ATPase. (2/104)

The ATPase activity of ArsA, the catalytic subunit of the plasmid-encoded, ATP-dependent extrusion pump for arsenicals and antimonials in Escherichia coli, is allosterically activated by arsenite or antimonite. Magnesium is essential for ATPase activity. To examine the role of Asp45, mutants were constructed in which Asp45 was changed to Glu, Asn, or Ala. Cells expressing these mutated arsA genes lost arsenite resistance to varying degrees. Purified D45A and D45N enzymes were inactive. The purified D45E enzyme exhibited approximately 5% of the wild type activity with about a 5-fold decrease in affinity for Mg2+. Intrinsic tryptophan fluorescence was used to probe Mg2+ binding. ArsA containing only Trp159 exhibited fluorescence enhancement upon the addition of MgATP, which was absent in D45N and D45A. As another measure of conformation, limited trypsin digestion was used to estimate the surface accessibility of residues in ArsA. ATP and Sb(III) synergistically protected wild type ArsA from trypsin digestion. Subsequent addition of Mg2+ increased trypsin sensitivity. D45N and D45A remained protected by ATP and Sb(III) but lost the Mg2+ effect. D45E exhibited an intermediate Mg2+ response. These results indicate that Asp45 is a Mg2+-responsive residue, consistent with its function as a Mg2+ ligand. (+info)

The ATPase mechanism of ArsA, the catalytic subunit of the arsenite pump. (3/104)

The ArsA ATPase is the catalytic subunit of a novel arsenite pump, with two nucleotide-binding consensus sequences in the N- and C-terminal halves of the protein. The single tryptophan-containing Trp159 ArsA was used to elucidate the elementary steps of the ATPase mechanism by fluorescence stopped-flow experiments. The binding and hydrolysis of MgATP is a multistep process with a minimal kinetic mechanism (Mechanism 1). A notable feature of the reaction is that MgATP binding induces a slow transient increase in fluorescence of ArsA, which is independent of the ATP concentration, indicative of the build-up of a pre-steady state intermediate. This finding, coupled with a phosphate burst, implies that the steady-state intermediate builds up subsequent to product release. We propose that the rate-limiting step is an isomerization between different conformational forms of ArsA. kcat is faster than the phosphate burst, indicating that both nucleotide binding sites of ArsA are catalytic. Consistent with this interpretation, approximately 2 mol of phosphate are released per mole of ArsA during the phosphate burst. (+info)

The anion-stimulated ATPase ArsA shows unisite and multisite catalytic activity. (4/104)

ArsA, an anion-stimulated ATPase, consists of two nucleotide binding domains, A1 in the N terminus and A2 in the C terminus of the protein, connected by a linker. The A1 domain contains a high affinity ATP binding site, whereas the A2 domain has low affinity and it requires the allosteric ligand antimonite for binding ATP. ArsA is known to form a UV-activated adduct with [alpha-(32)P]ATP in the linker region. This study shows that on addition of antimonite, much more adduct is formed. Characterization of the nature of the adduct suggests that it is between ArsA and ADP, instead of ATP, indicating that the adduct formation reflects hydrolysis of ATP. The present study also demonstrates that the A1 domain is capable of carrying out unisite catalysis in the absence of antimonite. On addition of antimonite, multisite catalysis involving both A1 and A2 sites occurs, resulting in a 40-fold increase in ATPase activity. Studies with mutant proteins suggest that the A2 site may be second in the sequence of events, so that its role in catalysis is dependent on a functional A1 site. It is also proposed that ArsA goes through an ATP-bound and an ADP-bound conformation, and the linker region, where ADP binds under both unisite and multisite catalytic conditions, may play an important role in the energy transduction process. (+info)

Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance. (5/104)

Four Nudix hydrolase genes, ysa1 from Saccharomyces cerevisiae, orf209 from Escherichia coli, yqkg from Bacillus subtilis, and hi0398 from Hemophilus influenzae were amplified, cloned into an expression vector, and transformed into E. coli. The expressed proteins were purified and shown to belong to a subfamily of Nudix hydrolases active on ADP-ribose. Comparison with other members of the subfamily revealed a conserved proline 16 amino acid residues downstream of the Nudix box, common to all of the ADP-ribose pyrophosphatase subfamily. In this same region, a conserved tyrosine designates another subfamily, the diadenosine polyphosphate pyrophosphatases, while an array of eight conserved amino acids is indicative of the NADH pyrophosphatases. On the basis of these classifications, the trgB gene, a tellurite resistance factor from Rhodobacter sphaeroides, was predicted to designate an ADP-ribose pyrophosphatase. In support of this hypothesis, a highly specific ADP-ribose pyrophosphatase gene from the archaebacterium, Methanococcus jannaschii, introduced into E. coli, increased the transformant's tolerance to potassium tellurite. (+info)

Mechanism of the ArsA ATPase. (6/104)

The ArsAB ATPase confers metalloid resistance in Escherichia coli by pumping toxic anions out of the cells. This transport ATPase shares structural and perhaps mechanism features with ABC transporters. The ArsAB pump is composed of a membrane subunit that has two groups of six transmembrane segments, and the catalytic subunit, the ArsA ATPase. As is the case with many ABC transporters, ArsA has an internal repeat, each with an ATP binding domain, and is allosterically activated by substrates of the pump. The mechanism of allosteric activation of the ArsA ATPase has been elucidated at the molecular level. Binding of the activator produces a conformational change that forms a tight interface of the nucleotide binding domains. In the rate-limiting step in the overall reaction, the enzyme undergoes a slow conformational change. The allosteric activator accelerates catalysis by increasing the velocity of this rate-limiting step. We postulate that similar conformational changes may be rate-limiting in the mechanism of ABC transporters. (+info)

Trimeric ring-like structure of ArsA ATPase. (7/104)

ArsA protein is the soluble subunit of the Ars anion pump in the Escherichia coli membrane which extrudes arsenite or antimonite from the cytoplasm. The molecular weight of the subunit is 63 kDa. In the cell it hydrolyzes ATP, and the energy released is used by the membrane-bound subunit ArsB to transport the substrates across the membrane. We have obtained two-dimensional crystals of ArsA in the presence of arsenite on negatively-charged lipid monolayer composed of DMPS and DOPC. These crystals have been studied using electron microscopy of negatively-stained specimens followed by image processing. The projection map obtained at 2.4 nm resolution reveals a ring-like structure with threefold symmetry. Many molecular assemblies with the same ring-shape and dimensions were also seen dispersed on electron microscopy grids, prepared directly from purified ArsA protein solution. Size-exclusion chromatography of the protein sample with arsenite present revealed that the majority of the protein particles in solution have a molecular weight of about 180 kDa. Based on these experiments, we conclude that in solution the ArsA ATPase with substrate bound is mainly in a trimeric form. (+info)

The chromosomal arsenic resistance genes of Thiobacillus ferrooxidans have an unusual arrangement and confer increased arsenic and antimony resistance to Escherichia coli. (8/104)

The chromosomal arsenic resistance genes of the acidophilic, chemolithoautotrophic, biomining bacterium Thiobacillus ferrooxidans were cloned and sequenced. Homologues of four arsenic resistance genes, arsB, arsC, arsH, and a putative arsR gene, were identified. The T. ferrooxidans arsB (arsenite export) and arsC (arsenate reductase) gene products were functional when they were cloned in an Escherichia coli ars deletion mutant and conferred increased resistance to arsenite, arsenate, and antimony. Therefore, despite the fact that the ars genes originated from an obligately acidophilic bacterium, they were functional in E. coli. Although T. ferrooxidans is gram negative, its ArsC was more closely related to the ArsC molecules of gram-positive bacteria. Furthermore, a functional trxA (thioredoxin) gene was required for ArsC-mediated arsenate resistance in E. coli; this finding confirmed the gram-positive ArsC-like status of this resistance and indicated that the division of ArsC molecules based on Gram staining results is artificial. Although arsH was expressed in an E. coli-derived in vitro transcription-translation system, ArsH was not required for and did not enhance arsenic resistance in E. coli. The T. ferrooxidans ars genes were arranged in an unusual manner, and the putative arsR and arsC genes and the arsBH genes were translated in opposite directions. This divergent orientation was conserved in the four T. ferrooxidans strains investigated. (+info)

... superfamily ARC3 family Arsenite-Antimonite efflux Arsenite-transporting ATPase Solute carrier family Active transport ATP- ... Arsenite or Antimonite (out). The overall reaction catalyzed by ArsB-ArsA is: Arsenite or Antimonite (in) + ATP ⇌ Arsenite or ... "Families of soft-metal-ion-transporting ATPases". Journal of Bacteriology. 181 (19): 5891-7. doi:10.1128/JB.181.19.5891- ... Arsenite resistance (Ars) efflux pumps of bacteria may consist of two proteins, ArsB (TC# 2.A.45.1.1; the integral membrane ...

https://en.wikipedia.org/wiki/ArsB_and_ArsAB_transporters

Arsenic toxicity Arsenite-transporting ATPase Solute carrier family Active transport ATP-binding cassette transporter Hasgekar ... Arsenite-antimonite transporters are membrane transporters that pump arsenite or antimonite out of a cell. Antimonite is the ... an ATPase, must be superimposed onto ArsB. Arsenite and antimonite can also be pumped out of the cell by members of the ARC3 ... which can participate in both secondary transport or primary active transport. Based on operon analyses, Arc3 homologues may ...

https://en.wikipedia.org/wiki/Arsenite-antimonite_transporter

In enzymology, an arsenite-transporting ATPase (EC 3.6.3.16) is an enzyme that catalyzes the chemical reaction ATP + H2O + ... and arsenite, whereas its 3 products are ADP, phosphate, and arsenite. This enzyme belongs to the family of hydrolases, ... Arsenite-Antimonite efflux Silver S, Misra TK, Laddaga RA (1989). "DNA sequence analysis of bacterial toxic heavy metal ... The systematic name of this enzyme class is ATP phosphohydrolase (arsenite-exporting). As of late 2007, 3 structures have been ...

https://en.wikipedia.org/wiki/Arsenite-transporting_ATPase

It is required for the detoxification of arsenate, arsenite, and antimonite. This system transports arsenite and antimonite out ... thus increasing its ATPase activity at lower concentrations of arsenite and enhancing the rate of arsenite extrusion. Carlin A ... This two-subunit enzyme produces resistance to arsenite and antimonite. Arsenate, however, must first be reduced to arsenite ... ArsA and ArsB form an anion-translocating ATPase. The ArsB protein is distinguished by its overall hydrophobic character, in ...

https://en.wikipedia.org/wiki/Ars_operon

V-type ATPase and A-type ATPase superfamily 3.A.3 The P-type ATPase Superfamily 3.A.4 The Arsenite-Antimonite efflux family 3.A ... Family 3.B.1 The Na+-transporting Carboxylic Acid Decarboxylase (NaT-DC) Family 3.C.1 The Na+ Transporting ... Family 1.D.58 The Anion Transporting Prodigiosene (Prodigiosene) Family 1.D.59 The Anion Transporting Perenosin (Perenosin) ... See electron transport chain. 3.D.1 The H+ or Na+-translocating NADH Dehydrogenase ("complex I") family 3.D.2 The Proton- ...

https://en.wikipedia.org/wiki/Transporter_Classification_Database

An underlying mechanism by which lead is able to cause harm is its ability to be transported by calcium ATPase pumps across the ... As a metabolite of arsenic, arsenite is formed after ingestion of arsenic and has shown significant toxicity to neurons within ... This is important as neurotransmitter transport can be impaired through vesicular transport inhibition, resulting in diminished ... This barrier creates a tight hydrophobic layer around the capillaries in the brain, inhibiting the transport of large or ...

https://en.wikipedia.org/wiki/Neurotoxin

H+-transporting two-sector ATPase EC 3.6.3.15: Na+-transporting two-sector ATPase EC 3.6.3.16: arsenite-transporting ATPase EC ... teichoic-acid-transporting ATPase EC 3.6.3.41: heme-transporting ATPase EC 3.6.3.42: beta-glucan-transporting ATPase EC 3.6. ... steroid-transporting ATPase EC 3.6.3.46: cadmium-transporting ATPase EC 3.6.3.47: fatty-acyl-CoA-transporting ATPase EC 3.6. ... vitamin B12-transporting ATPase EC 3.6.3.34: iron-chelate-transporting ATPase EC 3.6.3.35: manganese-transporting ATPase EC 3.6 ...

https://en.wikipedia.org/wiki/List_of_EC_numbers_(EC_3)

It has also been proposed that microtubules play a role in the formation of stress granules, maybe by transporting granule ... Jain S, Wheeler JR, Walters RW, Agrawal A, Barsic A, Parker R (January 2016). "ATPase-Modulated Stress Granules Contain a ... Chang YW, Huang YS (2014). "Arsenite-activated JNK signaling enhances CPEB4-Vinexin interaction to facilitate stress granule ... In yeast, catalytic ded1 mutant alleles give rise to constitutive stress granules ATPase-deficient DDX3X (the mammalian homolog ...

https://en.wikipedia.org/wiki/Stress_granule

Protons are transported across the membrane by the initial NADH reductase, quinones, and nitrous oxide reductase to produce the ... In all cases, however, a proton motive force is generated and used to drive ATP production via an ATPase. Most photosynthetic ... reduction to arsenite (AsO3− 3) Uranyl ion ion (UO2+ 2) reduction to uranium dioxide (UO 2) A number of organisms, instead of ... This means that these organisms do not use an electron transport chain to oxidize NADH to NAD+ and therefore must have an ...

https://en.wikipedia.org/wiki/Microbial_metabolism

... metal transporting P1-type ATPases and a chemiosmotic antiporter efflux system similar to CzcCBA of Cupriavidus metallidurans. ... MM-1 isolated from a soil and identification of arsenite oxidase gene". Journal of Hazardous Materials. 262: 997-1003. doi: ... Bahar, Md Mezbaul; Megharaj, Mallavarapu; Naidu, Ravi (2013-11-15). "Kinetics of arsenite oxidation by Variovorax sp. ...

https://en.wikipedia.org/wiki/Variovorax_paradoxus

https://en.wikipedia.org/wiki/Arsenite-transporting_ATPase

DR GO; GO:0015446; F:arsenite-transporting ATPase activity; IEA:InterPro. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. ... pump-driving ATPase homolog;AltName: Full=Arsenite-stimulated ATPase; DE (AEDAE_4253.PE8). GN ORFNames=AAEL011136; OS AEDES ... DR InterPro; IPR016300; Arsenical_pump_ATPase_ArsA. DR TIGRFAMs; TIGR00345; ArsA; 1. DR HOGENOMDNA; AEDAE_4253.PE8; -. KW ... 3, Last annotation update) DE RecName: Full=ATPase ASNA1 homolog; EC=3.6.- -;AltName: Full=Arsenical DE ...

http://pbil.univ-lyon1.fr/cgi-bin/acnuc-search-id?query=AEDAE_4253_PE8&db=HOGENOM&ident=ACNUC30567

Arsenite-translocating ATPase) (Arsenical resistance ATPase) (Arsenite-transporting ATPase) (ARSA) (ASNA-I). Os02t0745000-01 ... arsenite/tail-anchored protein-transporting ATPase [EC:7.3.2.7 7.3.-.-] K01551 arsA; arsenite/tail-anchored protein- ... Os01t0919800-01 Os01g0919800; Similar to Efflux carrier of polar auxin transport. Os02t0743400-01 Os02g0743400; Auxin transport ... Os05t0472700-01 Os05g0472700; Similar to Metal transport protein. Os03t0667300-00 Os03g0667300; Similar to Metal transport ...

https://www.genome.jp/kegg-bin/get_htext?dosa00001+Os09t0286400-01

... a member of the ABC superfamily of transport ATPases. Arsenate is reduced to arsenite via the ArsC arsenate reductase. Arsenite ... They have previously been described as CPx-type ATPases (55) or soft-metal-transporting P-type ATPases (42). These ATPases have ... SOFT-METAL-TRANSPORTING P-TYPE ATPASES. The superfamily of P-type ATPases is a ubiquitous group of proteins involved in ... Soft-metal-transporting P-type ATPases can be further divided into subgroups that contain Cu(I)/Ag(I)-translocating ATPases and ...

https://jb.asm.org/content/181/19/5891?ijkey=e890b9ddd79fd5a7126ac85a798ff832d220610b&keytype2=tf_ipsecsha

ATPase II (ATP8A1) can display an ATP-dependent phospholipid translocase activity. However, the function of ATP8A1 in ... Keywords: Apoptosis, Arsenite Transporting ATPases, Hepatocytes, phosphatidylinositol 3-kinase. Full Text Order reprints Export ... Over-Expression of ATPase II Alleviates Ethanol-Induced Hepatocyte Injury in HL-7702 Cells. Qing Li, Yan Yang, Ying Liu ... ATPase II (ATP8A1) can display an ATP-dependent phospholipid translocase activity. However, the function of ATP8A1 in ...

https://www.medscimonit.com/abstract/index/idArt/910254

... arsenite/tail-anchored protein-transporting ATPase [EC:7.3.2.7 7.3.-.-] K06316 RFT1; oligosaccharide translocation protein RFT1 ... 154215 NKAIN2; sodium/potassium transporting ATPase interacting 2 79570 NKAIN1; sodium/potassium transporting ATPase ... 286183 NKAIN3; sodium/potassium transporting ATPase interacting 3 128414 NKAIN4; sodium/potassium transporting ATPase ... sodium/potassium-transporting ATPase subunit beta-1-interacting protein K22735 NKAIN; sodium/potassium-transporting ATPase ...

https://www.genome.jp/kegg-bin/get_htext?hsa00001+116337

Anion-transporting ATPase. Catalyzes the extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low ... Miscellaneous arsenites. Alternative Parents. Trivalent inorganic arsenic compounds / Metalloid salts / Inorganic salts. ... Arsenite / Trivalent inorganic arsenic compound / Inorganic salt / Inorganic metalloid salt / Inorganic arsenic compound. ... These are inorganic compounds in which the largest metallic oxoanion is arsenite, to which either no atom or a non metal atom ...

https://www.drugbank.ca/drugs/DB04456

https://en.wikipedia.org/wiki/Arsenite-antimonite_transporter

... arsenite/tail-anchored protein-transporting ATPase [EC:7.3.2.7 7.3.-.-] K01551 arsA; arsenite/tail-anchored protein- ... biopolymer transport protein ExbB K03561 exbB; biopolymer transport protein ExbB K03561 exbB; biopolymer transport protein ExbB ... biopolymer transport protein TolQ K03562 tolQ; biopolymer transport protein TolQ K03560 tolR; biopolymer transport protein TolR ... biopolymer transport protein TolR K03560 tolR; biopolymer transport protein TolR K03560 tolR; biopolymer transport protein TolR ...

https://www.kegg.jp/kegg-bin/get_htext?mbd00001+MEBOL_000467

Once absorbed, inorganic arsenic is transported to the liver, where it is reduced to arsenite and then methylated. Most ... Vanadium also stimulates cell proliferation and differentiation and inhibits various ATPases, phosphatases, and phosphoryl- ... Nickel is transported through the blood bound primarily to albumin. Most organs and tissues do not accumulate nickel, but in ... ganic forms that contain trivalent arsenite (III) or pentavalent arsenate (V) hav- ing the greatest toxicological significance ...

https://www.nap.edu/read/11537/chapter/47

The arsenic resistance efflux system transports arsenite, using alternatively either a two-component (ArsA and ArsB) ATPase or ... encode P-type ATPases that are more similar to the bacterial CadA and CopB ATPases than to eukaryote ATPases that pump ... Other systems, such as copper transport ATPases and metallothionein cation-binding proteins, are only known from chromosomal ... The CadA ATPase protein has been labeled with 32P from gamma-32P-ATP and drives ATP-dependent Cd2+ uptake by inside-out ...

https://www.ncbi.nlm.nih.gov/pubmed/8905098?dopt=Abstract

The arsenic resistance efflux system transports arsenite [As(III)], alternatively using either a double-polypeptide (ArsA and ... Some of the efflux systems are ATPases and others are chemiosmotic cation/proton antiporters. The Cd(2+)-resistance ATPase of ... encode P-type ATPases that are more similar to bacterial CadA than to other ATPases from eukaryotes. ... to arsenite [As(III)], the substrate of the efflux system. The triple-polypeptide Czc (Cd2+, Zn2+ and Co2+) chemiosmotic efflux ...

https://pubmed.ncbi.nlm.nih.gov/8991852/?dopt=Abstract

The recent clusters include K+ channel proteins (285 members), ABC transporters (152 members), cation-transporting ATPases (59 ... arsenite, and chromate ion were identified, and there are no predicted anion channels. The reason for the difference in the ... and phospholipid-transporting ATPases (20 members). The expansion of the K+ channel proteins, which are VIC-type transporters, ... Most of these are channel-type transporters and cation-transporting P-type ATPases. Interestingly, despite the apparent massive ...

https://phylogenomics.blogspot.com/2007/03/power-of-open-access-ii-posting-my-open.html

It is known that arsenite salts are more toxic than arsenate as it binds with vicinal thiols in pyruvate dehydrogenase while ... It is normally present as arsenate under oxic conditions while arsenite is predominant under reducing condition. The major ... arsenate that enters the cell will be reduced to arsenite by ArsC gene before it is transported out of the cell by ArsB gene [ ... translocating ATPase, an ATP-driven efflux pump) [3, 53, 64]. Moreover, some detoxification operons also contain two additional ...

https://www.hindawi.com/journals/bmri/2014/503784/

... an ATPase that associates with ArsB and links arsenite export to ATP hydrolysis)22. The highest sequence identities obtained ... 11 Arsenate entering the cell is reduced to the more toxic arsenite by ArsC, and is then transported out of the cell via ArsB ( ... Ji G, Silver S. Reduction of arsenate to arsenite by the ArsC protein of the arsenic resistance operon of Staphylococcus aureus ... Sato T, Kobayashi Y. The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite. J ...

http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0038-23532010000100018&lng=es&nrm=iso&tlng=es

Arsenite-transporting ATPase 3.6.3.17 Monosaccharide-transporting ATPase 3.6.3.18 Transferred entry: 7.5.2.2 3.6.3.19 ... Chloride-transporting ATPase 3.6.3.12 Transferred entry: 7.2.2.6 3.6.3.13 Transferred entry: 3.6.3.1 3.6.3.14 Transferred entry ... Vesicle-fusing ATPase 3.6.4.7 Peroxisome-assembly ATPase 3.6.4.8 Transferred entry: 5.6.1.5 3.6.4.9 Transferred entry: 5.6.1.7 ... Cadmium-exporting ATPase 3.6.3.4 Transferred entry: 7.2.2.9 3.6.3.5 Transferred entry: 7.2.2.12 3.6.3.6 Transferred entry: 7.1. ...

https://enzyme.expasy.org/EC/3.-.-.-

ATP-Binding Cassette Transporters/genetics/*metabolism Animals Antigen Presentation/genetics Arsenite Transporting ATPases/ ... BNLF2a binds directly to the core TAP complex arresting the ATP-binding cassette transporter in a transport-incompetent ... viral BNLF2a protein hijacks the tail-anchored protein insertion machinery to block antigen processing by the transport complex ... viral BNLF2a protein hijacks the tail-anchored protein insertion machinery to block antigen processing by the transport complex ...

http://epubs.surrey.ac.uk/828849/

... membrane-associated arsenite permeases (ArsB or ACR3), and arsenite-translocating ATPases (ArsA). Other genes for arsenate ... Heavy Metal Transport and Detoxification. A. richmondensis encoded and expressed many genes related to heavy metal transport ... Overall, 24 metal transport and detoxification proteins were measured only in streamer biofilms, and an additional eight ... However, A. richmondensis genes involved in responding to these harsh conditions (i.e., metal transport and detoxification and ...

https://www.frontiersin.org/articles/10.3389/fmicb.2016.00238/full

Arsenite Transporting ATPases/chemistry. *Arsenite Transporting ATPases/genetics. *Arsenite Transporting ATPases/metabolism* ... The highly conserved, 40 kDa ATPase subunit of TRC (which we termed TRC40) was identified as Asna-1. TRC40/Asna-1 interacts ... Consequently, an ATPase-deficient mutant of TRC40/Asna-1 dominantly inhibited TA protein insertion selectively without ...

https://www.ncbi.nlm.nih.gov/pubmed/17382883?dopt=Abstract

Arsenite Transporting ATPase Arsenite-Translocating ATPase Arsenite-Transporting ATPase Transporting ATPases, Arsenite ... Arsenite Transporting ATPase. Arsenite-Translocating ATPase. Arsenite-Transporting ATPase. Transporting ATPases, Arsenite. ... Arsenite Transporting ATPases Entry term(s). ATPase, Arsenite-Translocating ATPase, Arsenite-Transporting ATPases, Arsenite ... Arsenite Transporting ATPases Entry term(s):. ATPase, Arsenite-Translocating. ATPase, Arsenite-Transporting. ATPases, Arsenite ...

https://decs.bvsalud.org/en/ths/resource/?id=52213&filter=&q=%E2%8C%A9_another=fr%E2%8C%A9_another=fr

Arsenite Transporting ATPases * Biochemistry & Molecular Biology * Cell Membrane * Drug Resistance, Microbial * Enzyme ... Interaction of the catalytic and the membrane subunits of an oxyanion-translocating ATPase. Academic Article * Authors Version ... Addition of ATP had little affect on membrane binding of the ArsA ATPase subunit. In the presence or absence of the anionic ... In the absence of arsenite or antimonite an apparent positive cooperativity in the binding of the ArsA protein to membrane ...

https://findscholars.unh.edu/display/publication90580

A few additional transport functions encoded on pPB12 include a CDS for a P-type ATPase Mg2+ importer (Nham_4377), a predicted ... A putative arsenite oxidase gene and accessory genes were also located on pPB12 (Nham_4425 to Nham_4432), although it is ... As is the situation for most potential organic substrates, candidate genes for the transport and uptake of the aforementioned ... ABC transport systems. Although it would be a costly solution given the ATP dependence of all the enzymes, N. hamburgensis may ...

https://aem.asm.org/content/74/9/2852

Hypothetical protein; Anion-transporting ATPase. Catalyzes the extrusion of arsenite (By similarity) (396 aa) ... Hypothetical protein; Anion-transporting ATPase. Catalyzes the extrusion of arsenite (By similarity) ... Arsenate reductase; Reduces arsenate [As(V)] to arsenite [As(III)] using glutathione and glutaredoxin as sources of reducing ... Arsenate reductase; Reduces arsenate [As(V)] to arsenite [As(III)] using glutathione and glutaredoxin as sources of reducing ...

https://string-db.org/network/1148.SYNGTS_1833

anion transport. go/ biological_process. arsenite-transmembrane transporting ATPase activity. go/ molecular_function. membrane ... Oxyanion-translocating ATPase. cog/ cog. ATP binding. go/ molecular_function. ... anion transport. go/ biological_process. arsenite-transmembrane transporting ATPase activity. go/ molecular_function. ...

http://networks.systemsbiology.net/hal/gene/VNG0365G

Arsenite Transporting ATPases. *Ca(2+) Mg(2+)-ATPase. *Calcium-Transporting ATPases. *Chaperonins ... The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes ... Pathogenic mutations in the ALS gene CCNF cause cytoplasmic mislocalization of Cyclin F and elevated VCP ATPase activity. Hum ... Cycle Restores Wild-Type Function in the Lipopolysaccharide Transporter and Shows that ATP Binding Triggers Transport. mBio. ...

https://connects.catalyst.harvard.edu/Profiles/display/Concept/Adenosine%20Triphosphatases

Arsenite-transporting ATPase. *Arsole. *Arthropod. *Artificial respiration. *Asbestos. *Ascophyllum nodosum. *Ashermans ...

https://www.wikidoc.org/index.php/Category:CS1_errors:_dates

The arsA gene codes for a unique ATPase which binds to the ArsB membrane protein forming an anion transporting arsenite pump [ ... Siebers A, Altendorf K: K+-translocating Kdp-ATPases and other bacterial P-type ATPases. Alkali cation transport systems in ... The merR gene controls regulation of the operon, merT and merP transport of the mercury ions and merA reduction of the mercury ... Ji G, Silver S: Reduction of arsenate to arsenite by the ArsC protein of the arsenic resistance operon of Staphylococcus aureus ...

https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-9-242

Mediates the transport of S-(2,4-dinitrophenyl)-glutathione (DNP-GS), GSSG, cyanidin 3-glucoside-GS (C3G-GS) and metolachlor-GS ... ATPase activity Source: InterPro. *ATPase-coupled arsenite transmembrane transporter activity Source: TAIRInferred from direct ... arsenite transport Source: TAIRInferred from direct assayi*. "Arsenic tolerance in Arabidopsis is mediated by two ABCC-type ... abscisic acid D-glucopyranosyl ester transmembrane transport Source: TAIRInferred from direct assayi*. "Vacuolar transport of ...

https://www.uniprot.org/uniprot/Q9C8G9

3.A.3 The P-type ATPase (P-ATPase) Superfamily. 3.A.4 The Arsenite-Antimonite (ArsAB) Efflux Family ... Transport systems are included in this subclass if they hydrolyze the diphosphate bond of inorganic pyrophosphate, ATP, or ... 3.A.2 The H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) Superfamily ... The transport protein may or may not be transiently phosphorylated, but the substrate is not phosphorylated. ...

http://www.tcdb.org/search/result.php?tc=3.A

Arsenite Transporting ATPases. *Asparagine. *Autophagy. *Bacteria. *Bacterial Proteins. *Base Sequence. *Basic Helix-Loop-Helix ...

https://profiles.umassmed.edu/display/133243/network/researchareas/cloud

ArsB is a secondary transporter that catalyzes efflux of the arsenite anion from cells ( 14 ) (Fig. 1 ). (asm.org)
ArsA is an As(III)/Sb(III)-stimulated ATPase ( 46 ) that associates with ArsB to form an extrusion pump that is more efficient than ArsB alone ( 14 ) (Fig. 1 ). (asm.org)
ArsB family (TC# 2.A.45) ArsAB Family (TC# 3.A.4) Arsenical resistance-3 (ARC3) family (TC#2.A.59) The membrane transporter ArsB can function as a secondary carrier or as a primary active transporter, in which case ArsA, an ATPase, must be superimposed onto ArsB. (wikipedia.org)
The arsenic resistance efflux system transports arsenite, using alternatively either a two-component (ArsA and ArsB) ATPase or a single polypeptide (ArsB) functioning as a chemiosmotic transporter. (nih.gov)
Sequence analysis of a clone conferring resistance to both sodium arsenite and sodium arsenate revealed homologues to the arsR (regulatory repressor), arsB (membrane located arsenite pump), arsC (arsenate reductase), arsD (second regulatory repressor and a metallochaperone) and arsA (ATPase) genes from known arsenic resistance operons. (scielo.org.za)
arsR (transcriptional repressor), arsC (arsenate reductase) and arsB (arsenite efflux membrane protein). (scielo.org.za)
The arsenical pump is an ion-translocating ATPase which consists of two polypeptide components, the ArsA and ArsB proteins. (unh.edu)
In the absence of arsenite or antimonite an apparent positive cooperativity in the binding of the ArsA protein to membrane vesicles containing the ArsB protein was observed. (unh.edu)
Arsenic is given more emphasis, as enzymes in the periplasm and cytoplasm are included as well as classes of transporters ArsA/ArsB ATPase efflux/influx pump. (eco-bgri.com)
The arsB efflux transport systems extend from the cytoplasm across the outer membrane of Gram-negative bacteria. (eco-bgri.com)
Fig.3 Sequence alignments of the arsenite-oxidizing bacterial to proteins of the arsB family. (eco-bgri.com)
The arsB gene, which encodes a putative arsenite-specific efflux pump was highly induced by As 3+ and As 5+ ions, while other metal salts provoked insignificant transcript level increase. (springer.com)
Moreover, the genetic background of the silA (silver-efflux pump) and the presence of other acquired heavy metal tolerance genes, pcoD (copper-efflux pump), arsB (arsenite-efflux pump), terF (tellurite resistance protein), and merA (mercuric reductase) were also investigated. (frontiersin.org)

The highly conserved, 40 kDa ATPase subunit of TRC (which we termed TRC40) was identified as Asna-1. (nih.gov)
Addition of ATP had little affect on membrane binding of the ArsA ATPase subunit. (unh.edu)
Interaction of ATP binding sites in the ArsA ATPase, the catalytic subunit of the Ars pump. (semanticscholar.org)
article{Li1996InteractionOA, title={Interaction of ATP binding sites in the ArsA ATPase, the catalytic subunit of the Ars pump. (semanticscholar.org)
The ArsA ATPase is the catalytic subunit of the Ars pump that catalyzes arsenical extrusion in Escherichia coli, thus providing resistance. (semanticscholar.org)
Antimonite regulation of the ATPase activity of ArsA, the catalytic subunit of the arsenical pump. (semanticscholar.org)

Arsenite-antimonite transporters are membrane transporters that pump arsenite or antimonite out of a cell. (wikipedia.org)
Among these, there are the P-type ATPase cation transporters, but not the sodium/ hydrogen exchanger family. (beds.ac.uk)
Bacteria have developed resistance against arsenic by using detoxification mechanisms such as oxidation of arsenite to arsenate with the aid of an enzyme arsenite oxidase and in the process gain energy, reduction of arsenate to arsenite through phosphate transporters by a reductase enzyme arsenate reductase and methylation of arsenite with the enzyme S-adenosylmethionine methyltransferases (SAM). (innovativepublication.com)

Arsenic toxicity Arsenite-transporting ATPase Solute carrier family Active transport ATP-binding cassette transporter Hasgekar, N. (wikipedia.org)
BNLF2a binds directly to the core TAP complex arresting the ATP-binding cassette transporter in a transport-incompetent conformation. (surrey.ac.uk)
Simpson BW, Pahil KS, Owens TW, Lundstedt EA, Davis RM, Kahne D, Ruiz N. Combining Mutations That Inhibit Two Distinct Steps of the ATP Hydrolysis Cycle Restores Wild-Type Function in the Lipopolysaccharide Transporter and Shows that ATP Binding Triggers Transport. (harvard.edu)
If GlpF behaves as a nonsaturable transporter, a transport rate of 1umol of glycerol is transported per minute per mgr of cell protein (Heller, KB, et al.1980) . (igem.org)
While the extrusion of excess copper via the CopA copper ATPase and the CusCFBA transporter and the regulation of these systems appear fairly clear, there are still major open questions concerning the metallation of cuproenzymes. (springer.com)
An ion transporter, is a transmembrane protein that transports ions across a plasma membrane. (isoquercetin.net)
In addition, NtUBC1 tobacco displayed a reduced accumulation of Cd compared with the control, likely due to the higher expression of CAX3 (Ca2+/H+ exchanger) and the lower expression of IRT1 (iron-responsive transporter 1) and HMA-A and -B (heavy metal ATPase). (deepdyve.com)

Arsenite-Antimonite efflux Silver S, Misra TK, Laddaga RA (1989). (wikipedia.org)
Antimonite is the salt of antimony (Sb(III)) and has been found to significantly impact the toxicity of arsenite. (wikipedia.org)
As of early 2016, there are at least three known families of proteins known to participate in arsenite and antimonite efflux. (wikipedia.org)
Arsenite and antimonite can also be pumped out of the cell by members of the ARC3 family, a member of the BART superfamily, which can participate in both secondary transport or primary active transport. (wikipedia.org)
In the presence of arsenite or antimonite binding became hyperbolic, with a 10-fold decrease in the concentration of ArsA protein required for half-maximal binding, without any change in the stoichiometry of the complex. (unh.edu)

Anion-transporting ATPase. (string-db.org)

In Escherichia coli and other bacteria, arsenate [As(V)] is taken up by phosphate transport systems such as the ATP-coupled Pst pump ( 52 ), a member of the ABC superfamily of transport ATPases (Fig. 1 ). (asm.org)
Therefore, this study sees value in the analysis of whether homeopathically diluted glucose 30C has any effect on Escherichia coli exposed to arsenite stress. (jcimjournal.com)
Khuda-Bukhsh AR, De A, Das D, Dutta S, Boujedaini N. Analysis of the capability of ultra-highly diluted glucose to increase glucose uptake in arsenite-stressed bacteria Escherichia coli. (jcimjournal.com)

The CadA ATPase protein has been labeled with 32P from gamma-32P-ATP and drives ATP-dependent Cd2+ uptake by inside-out membrane vesicles. (nih.gov)
Consequently, an ATPase-deficient mutant of TRC40/Asna-1 dominantly inhibited TA protein insertion selectively without influencing other translocation pathways. (nih.gov)
The transport protein may or may not be transiently phosphorylated, but the substrate is not phosphorylated. (tcdb.org)
Overall, these results shed light on the SbmA-mediated internalization of peptide substrates and suggest that the transport of an unknown substrate(s) represents the function of this protein. (asm.org)
The network for 'regulation of intracellular protein transport' in your query organism is displayed on the left, if relationships are supported by the integrated data. (princeton.edu)

Transport systems are included in this subclass if they hydrolyze the diphosphate bond of inorganic pyrophosphate, ATP, or another nucleoside triphosphate, to drive the active uptake and/or extrusion of a solute or solutes. (tcdb.org)
Membrane-embedded transport proteins mediate the cellular uptake and extrusion of a wide diversity of solutes. (biomedcentral.com)
Odermatt A, Krapf R, Solioz M (1994) Induction of the putative copper ATPases, CopA and CopB, of Enterococcus hirae by Ag + and Cu 2+ , and Ag + extrusion by CopB. (springer.com)

Arsenic usually exists in four oxidation states: As −3 (arsine), As° (arsenic), As +3 (arsenite), and As +5 (arsenate) [ 4 , 10 ]. (hindawi.com)
In soil environment, arsenic is generally present in two oxidation states which are As +3 (arsenite) and As +5 (arsenate) and normally present as a mixture of As +3 (arsenite) and As +5 (arsenate) in air [ 2 ]. (hindawi.com)
6 In soils, arsenic is present in two oxidation states: As(III) (arsenite) and As(V) (arsenate). (scielo.org.za)
These data are summarized in a model that functionally integrates arsenite oxidation in the adaptive response to As(III) in this microorganism. (biomedcentral.com)
[6] [7] Arsenic exists typically in four oxidation states as arsenate (v), arsenite (III), arsenic (0) and arsine (-III), with arsenite and arsenate being the most common forms. (innovativepublication.com)
Generally, arsenic is known to be a toxic metal and bacterial have devised several mechanisms such as arsenite oxidation, arsenate reduction and methylation to degrade this toxic substance and reduce its toxic effects. (innovativepublication.com)

The three-component Czc (Cd2+, Zn2+, and CO2+) chemiosmotic efflux pump of soil microbes consists of inner membrane (CzcA), outer membrane (CzcC), and membrane-spanning (CzcB) proteins that together transport cations from the cytoplasm across the periplasmic space to the outside of the cell. (nih.gov)
The triple-polypeptide Czc (Cd2+, Zn2+ and Co2+) chemiosmotic efflux pump consists of inner membrane (CzcA), outer membrane (CzcC) and membrane-spanning (CzcB) proteins that together transport cations from the cytoplasm across the periplasmic space to the outside of the cell. (nih.gov)

E. coli were cultured to their log phase in standard Luria-Bertani medium and then treated with either 1 mmol/L or 2 mmol/L sodium arsenite, with or without supplementation of either 1% or 3% glucose, an ultra-highly diluted and agitated ethanolic solution (70%) of glucose (diluted 1060 times), glucose 30C or 70% ethanol (placebo) in the medium. (jcimjournal.com)
In this context, we evaluated the mode of action of chronic oral exposure of sodium arsenite as the source of arsenic at 5 mg/kg/BW with THC (80 mg/ kg/BW) for 28 days. (omicsonline.org)
Bi-inorganic form of As in drinking water are trivalent (As +3 ) such as arsenic trioxide and sodium arsenite and pentavalent (As +5 ) such as arsenic pentoxide and the various arsenates. (omicsonline.org)

Recently isolated genes defective in the human hereditary diseases of copper metabolism, Menkes syndrome and Wilson's disease, encode P-type ATPases that are more similar to the bacterial CadA and CopB ATPases than to eukaryote ATPases that pump different cations. (nih.gov)
The Cd(2+)-resistance ATPase of Gram-positive bacteria (CadA) is membrane cation pump homologous with other bacterial, animal and plant P-type ATPases. (nih.gov)
SEM images of arsenite-oxidizing bacterial OS-5 (AY952321), RW-28(DQ112333)and OS-19(AY866406) are shown. (eco-bgri.com)
Use of a bacterial two-hybrid system coupled to SEC-MALLS (size exclusion chromatography coupled with multiangle laser light scattering) analyses established that SbmA is a homodimer in the membrane, and treatment of the cells with arsenate or ionophores indicated that the peptide transport mediated by SbmA is driven by the electrochemical gradient. (asm.org)
Metabolic reconstructions of the "Pyropristinus" and Calescamantes populations showed that these organisms appear to be chemoorganoheterotrophs and have the genomic potential for aerobic respiration and oxidative phosphorylation via archaeal-like V-type, and bacterial F-type ATPases, respectively. (frontiersin.org)

In the latter case ATP hydrolysis again energizes transport. (wikipedia.org)
Efflux pumps that use the energy of ATP hydrolysis to pump arsenite across a membrane. (bvsalud.org)
The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. (harvard.edu)
Cation-transporting proteins that utilize the energy of ATP hydrolysis for the transport of CALCIUM. (childrensmercy.org)

Interaction of the catalytic and the membrane subunits of an oxyanion-translocating ATPase. (unh.edu)

Meanwhile, the reduction of arsenate into arsenite in the transition from aerobic to anoxic pore waters is often mediated by microbial activity, which includes detoxification and metabolic mechanisms [ 8 ]. (hindawi.com)

The third gene in the arsenic resistance system, arsC, encodes an enzyme that converts intracellular arsenate [As (V)] to arsenite [As (III)], the substrate of the efflux system. (nih.gov)

10 Some have two additional genes, arsD (second regulator and a metallochaperone) and arsA (ATPase), which confer higher resistance than the three-gene operon. (scielo.org.za)
Yu Y, Nakagawa T, Morohoshi A, Nakagawa M, Ishida N, Suzuki N, Aoki M, Nakayama K. Pathogenic mutations in the ALS gene CCNF cause cytoplasmic mislocalization of Cyclin F and elevated VCP ATPase activity. (harvard.edu)
In arsenite-exposed E. coli, the glucose uptake increased along with decreases in the specific activities of hexokinase and glucokinase, intracellular ATP and membrane potential and an increase in the gene expression level of glucose permease. (jcimjournal.com)
The ziaA gene, which encodes a Zn 2+ -transporting P-type ATPase showed a markedly increased mRNA level after incubation with Cd 2+ and arsenic ions, besides the expected induction by Zn 2+ ions. (springer.com)
Western blotting with anti-AoxB antibodies and quantitative RT-PCR experiments allowed us to demonstrate that the rpoN and dnaJ gene products are involved in the control of arsenite oxidase gene expression. (biomedcentral.com)
Phung LT, Ajlani G, Haselkorn R (1994) P-type ATPase from the cyanobacterium Synechococcus 7942 related to the human Menkes and Wilson disease gene products. (springer.com)
This means that polyphenols can have beneficial effects on the body including the regulation of blood pressure by reducing vascular contraction (22), and also through renal Na+ reabsorption via regulating ENaC gene expression (23-24) as well as through Na+,K+-ATPase activity (25). (isoquercetin.net)
The Wilson disease gene, a copper transporting ATPase (Atp7b), is responsible for the sequestration of Cu into secretory vesicles, and this function is exhibited by the orthologous Ccc2p in the yeast. (iyte.edu.tr)

In the present study, a differential transcriptome analysis was used to identify genes, including arsenite oxidase encoding genes, involved in the response of H. arsenicoxydans to As(III). (biomedcentral.com)
Transposon insertions resulting in a lack of arsenite oxidase activity disrupted aoxR and aoxS genes, showing that the aox operon transcription is regulated by the AoxRS two-component system. (biomedcentral.com)
These results reveal the existence of novel molecular regulatory processes governing arsenite oxidase expression in H. arsenicoxydans . (biomedcentral.com)
One of the most noticeable response to arsenic in H. arsenicoxydans is the ability of this bacterium to oxidize As(III) to As(V), a less toxic and less mobile form, via an arsenite oxidase activity. (biomedcentral.com)
Nevertheless, the molecular mechanisms involved in the control of arsenite oxidase expression remain largely unknown. (biomedcentral.com)

Other aspects of transport and resistance to those metal ions have been reviewed recently ( 44 , 45 , 53 , 55 , 62 ) and will not be discussed here. (asm.org)
Rather, those ions may be accumulated via other existing transport systems. (asm.org)
They are primarily found in prokaryotic organisms, where they play a role in protection against excess intracellular levels of arsenite ions. (bvsalud.org)
No unusual traits were observed in common salt-tolerance mechanisms, such as transport of inorganic ions or synthesis of compatible solutes. (beds.ac.uk)
Carbon sugars and ions are shown to be unable to be transported by GlpF (Heller, KB, et al.1980) . (igem.org)
The ion channels are pore-forming membrane proteins that allow transported ions to pass through the channel pore. (isoquercetin.net)

ArsC is a reductase that catalyzes electron transfer from reduced glutathione and glutaredoxin (in E. coli ) ( 18 ) or thioredoxin (in Staphylococcus aureus ) ( 23 , 24 ) to arsenate, reducing it to arsenite, the substrate of the efflux system. (asm.org)
The toxic effect of arsenic was also indicated by significantly decreased activities of membrane bound ATPases, enzymatic antioxidants like superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase and glucose-6-phosphate dehydrogenase along with non-enzymatic antioxidants like reduced glutathione, total sulfhydryl groups, vitamins C and E. Administration of THC exhibited a significant reversal of arsenic-induced toxicity in hepatic tissue. (omicsonline.org)

Inorganic trivalent arsenicals (arsenites, AsO 2 − ) are most potent toxicants [ 5 ]. (hindawi.com)

SIMILARITY: Belongs to the arsA ATPase family. (univ-lyon1.fr)
Role of conserved aspartates in the ArsA ATPase. (semanticscholar.org)

The ability of GlpF (overexpressed under IPTG induction) to transport As(III) was tested by an arsenite uptake assay . (igem.org)

This compound belongs to the class of inorganic compounds known as miscellaneous arsenites. (drugbank.ca)
These are inorganic compounds in which the largest metallic oxoanion is arsenite, to which either no atom or a non metal atom is bonded. (drugbank.ca)
The contamination of drinking water by the two inorganic forms, arsenite As(III) and arsenate As(V), has been reported in different parts of the world [ 3 ] and constitutes a major threat of public health. (biomedcentral.com)

In enzymology, an arsenite-transporting ATPase (EC 3.6.3.16) is an enzyme that catalyzes the chemical reaction ATP + H2O + arsenitein ⇌ {\displaystyle \rightleftharpoons } ADP + phosphate + arseniteout The 3 substrates of this enzyme are ATP, H2O, and arsenite, whereas its 3 products are ADP, phosphate, and arsenite. (wikipedia.org)

The CadA cadmium resistance ATPase of gram-positive bacteria and the CopB copper efflux system of Enterococcus hirae are homologous to P-type ATPases of animals and plants. (nih.gov)

download Transport and Intracellular Sequestration of Novel Thiosemicarbazone Chelators for the Treatment of Cancer. (ohlookprod.com)

Other systems, such as copper transport ATPases and metallothionein cation-binding proteins, are only known from chromosomal genes. (nih.gov)

FUNCTION: ATPase required for the post-translational delivery of CC tail-anchored (TA) proteins to the endoplasmic reticulum. (univ-lyon1.fr)
Here, we mined the transcriptome of three recently isolated strains of anaerobic fungi to identify membrane proteins responsible for sensing and transporting biomass hydrolysates within a competitive and rather extreme environment. (biomedcentral.com)
Organic Cation Transport Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)
A family of proteins involved in the transport of organic cations. (ucdenver.edu)
This graph shows the total number of publications written about "Organic Cation Transport Proteins" by people in this website by year, and whether "Organic Cation Transport Proteins" was a major or minor topic of these publications. (ucdenver.edu)
Below are the most recent publications written about "Organic Cation Transport Proteins" by people in Profiles. (ucdenver.edu)

These analyses revealed an increased expression of genes involved in cellular adhesion and biofilm maturation such as type IV pili, exopolysaccharide and electron transport chain components in ATCC 27853 compared with PAO1. (biomedcentral.com)

Molecular Biology and Toxicology of Metals provides a critical review and analysis of the current state of knowledge of metal ion transport and metabolism in prokaryotic and eukaryotic cellular systems. (google.com.my)
VCP (Ter94 in D. melanogaster and CDC48 in S. cerevisiae) is a member of the AAA (ATPase associated with various cellular activities) ATPase family. (atlasgeneticsoncology.org)
To determine whether the C-terminal domain affects the structure or activity of ATP8A2, we generated and characterized the transport function and cellular localization of a series of C-terminal truncation mutants. (pubmedcentralcanada.ca)

Unlike arsenate and arsenite, arsine is often available as highly toxic gases such as (CH 3 ) 3 and H 3 As and often present at low concentration in the environment [ 4 ]. (hindawi.com)

The systematic name of this enzyme class is ATP phosphohydrolase (arsenite-exporting). (wikipedia.org)

Unlike the yeast P4-ATPase Drs2, ATP8A2 is not regulated by phosphoinositides but undergoes phosphorylation on the serine residue within a CaMKII target motif. (pubmedcentralcanada.ca)

Arsenical_pump_ATPase_ArsA. (univ-lyon1.fr)

claimed that the arsenite binding site of the E. coli ArsA is not conserved in Arr4p. (tcdb.org)

Tottey S, Rich PR, Rondet SA et al (2001) Two Menkes-type atpases supply copper for photosynthesis in Synechocystis PCC 6803. (springer.com)
Hassani BK, Astier C, Nitschke W et al (2010) CtpA a copper-translocating P-type ATPase involved in the biogenesis of multiple copper-requiring enzymes. (springer.com)

ATPase II ( ATP8A1 ) can display an ATP-dependent phospholipid translocase activity. (medscimonit.com)

The structural similarities are thought to be the reason for the possibility of these elements to enter the cell by GlpF (Porquet, A, et al.2007) , GlpF facilitates transport of these compounds down there gradient (inside or outside the cell). (igem.org)
This barrier creates a tight hydrophobic layer around the capillaries in the brain, inhibiting the transport of large or hydrophilic compounds. (citizendium.org)

It is known that arsenite salts are more toxic than arsenate as it binds with vicinal thiols in pyruvate dehydrogenase while arsenate inhibits the oxidative phosphorylation process. (hindawi.com)
Arsenite has been reported as the most toxic and soluble form of arsenic when compared to arsenate, and it can bind with reactive sulfur atoms present in many enzymes, including enzymes which are involved in respiration [ 4 , 13 ]. (hindawi.com)
9 Under reducing or anaerobic conditions, the more toxic and soluble form of arsenic, arsenite, occurs. (scielo.org.za)
Lewinson O, Lee AT, Rees DC (2009) A P-type ATPase importer that discriminates between essential and toxic transition metals. (springer.com)
Arsenic is a highly toxic metalloid available in the environment mainly as arsenite or arsenate. (iyte.edu.tr)
Arsenic is a toxic metalloid that exists in two major forms (arsenate and arsenite). (innovativepublication.com)
[8] [9] Between the two common forms, arsenite is more toxic than arsenate due to its solubility and mobility. (innovativepublication.com)

As they degrade arsenic in the process, they use it for their own growth through energy generating processes such as arsenite acting as an electron donor while arsenate acts as an electron acceptor. (innovativepublication.com)

2003). While the D1 domain mediates oligomerization independent nucleotide binding, the D2 domain confers most of the ATPase activity (Wang et al. (atlasgeneticsoncology.org)
Deletion of the C-terminal 33 residues resulted in reduced phosphatidylserine-dependent ATPase activity, phosphatidylserine flippase activity, and neurite extension in PC12 cells. (pubmedcentralcanada.ca)

GlpF a aquaglycerol porin was found to import not only glycerol but also arsenite and arsenate. (igem.org)

Anatomy2

Organisms8

Diseases2

Chemicals and Drugs33

Psychiatry and Psychology2

Phenomena and Processes5

Technology, Industry, Agriculture1

Information Science2

Expression cloning for arsenite-resistance resulted in isolation of tumor-suppressor fau cDNA: possible involvement of the ubiquitin system in arsenic carcinogenesis. (1/104)

Asp45 is a Mg2+ ligand in the ArsA ATPase. (2/104)

The ATPase mechanism of ArsA, the catalytic subunit of the arsenite pump. (3/104)

The anion-stimulated ATPase ArsA shows unisite and multisite catalytic activity. (4/104)

Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance. (5/104)

Mechanism of the ArsA ATPase. (6/104)

Trimeric ring-like structure of ArsA ATPase. (7/104)

The chromosomal arsenic resistance genes of Thiobacillus ferrooxidans have an unusual arrangement and confer increased arsenic and antimony resistance to Escherichia coli. (8/104)

Arsenites

Sodium Compounds

Arsenite Transporting ATPases

Adenosine Triphosphatases

Arsenates

Arsenic

Proton-Translocating ATPases

Calcium-Transporting ATPases

Vacuolar Proton-Translocating ATPases

Ion Pumps

Antimony

Teratogens

Arsenate Reductases

Arsenicals

Plasma Membrane Calcium-Transporting ATPases

Molecular Sequence Data

Adenosine Triphosphate

Aquaglyceroporins

Antimony Potassium Tartrate

Amino Acid Sequence

Cation Transport Proteins

Alcaligenes faecalis

Gram-Negative Chemolithotrophic Bacteria

Ectothiorhodospiraceae

H(+)-K(+)-Exchanging ATPase

Sodium-Potassium-Exchanging ATPase

Archaeoglobus fulgidus

Vacuoles

Cell Membrane

Proton Pumps

Pteris

Sarcoplasmic Reticulum Calcium-Transporting ATPases

Saccharomyces cerevisiae

Ca(2+) Mg(2+)-ATPase

Arsenic Poisoning

Cadmium Chloride

Kinetics

Hydrolysis

Ralstonia

Biological Transport

Vanadates

Oxalobacteraceae

Copper

Saccharomyces cerevisiae Proteins

Multienzyme Complexes

Mutation

Alcohol Drinking

Phospholipid Transfer Proteins

Alcoholism

Ethanol

Carnitine Acyltransferases

Alcoholic Beverages

Phospholipids

Expression cloning for arsenite-resistance resulted in isolation of tumor-suppressor fau cDNA: possible involvement of the ubiquitin system in arsenic carcinogenesis. (1/104)

Asp45 is a Mg2+ ligand in the ArsA ATPase. (2/104)

The ATPase mechanism of ArsA, the catalytic subunit of the arsenite pump. (3/104)

The anion-stimulated ATPase ArsA shows unisite and multisite catalytic activity. (4/104)

Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance. (5/104)

Mechanism of the ArsA ATPase. (6/104)

Trimeric ring-like structure of ArsA ATPase. (7/104)

The chromosomal arsenic resistance genes of Thiobacillus ferrooxidans have an unusual arrangement and confer increased arsenic and antimony resistance to Escherichia coli. (8/104)

arsC - Arsenate reductase - Acinetobacter variabilis - arsC gene & protein

Ars operon - Wikipedia

ASNA1 (arsA arsenite transporter, ATP-binding, homolog 1 (bacterial))

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$Gentaur Molecular :Zyagen \ ASNA1 Mouse Monoclonal Antibody \ APO-000439-M03$ Gentaur Molecular :Zyagen \ ASNA1 Mouse Monoclonal Antibody \ APO-000439-M03