Arsenites: Inorganic salts or organic esters of arsenious acid.Sodium Compounds: Inorganic compounds that contain sodium as an integral part of the molecule.Arsenite Transporting ATPases: Efflux pumps that use the energy of ATP hydrolysis to pump arsenite across a membrane. They are primarily found in prokaryotic organisms, where they play a role in protection against excess intracellular levels of arsenite ions.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Arsenates: Inorganic or organic salts and esters of arsenic acid.Arsenic: A shiny gray element with atomic symbol As, atomic number 33, and atomic weight 75. It occurs throughout the universe, mostly in the form of metallic arsenides. Most forms are toxic. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), arsenic and certain arsenic compounds have been listed as known carcinogens. (From Merck Index, 11th ed)Proton-Translocating ATPases: Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.Calcium-Transporting ATPases: Cation-transporting proteins that utilize the energy of ATP hydrolysis for the transport of CALCIUM. They differ from CALCIUM CHANNELS which allow calcium to pass through a membrane without the use of energy.Vacuolar Proton-Translocating ATPases: Proton-translocating ATPases that are involved in acidification of a variety of intracellular compartments.Ion Pumps: A general class of integral membrane proteins that transport ions across a membrane against an electrochemical gradient.Antimony: A metallic element that has the atomic symbol Sb, atomic number 51, and atomic weight 121.75. It is used as a metal alloy and as medicinal and poisonous salts. It is toxic and an irritant to the skin and the mucous membranes.Teratogens: An agent that causes the production of physical defects in the developing embryo.Arsenate Reductases: Oxidoreductases that specifically reduce arsenate ion to arsenite ion. Reduction of arsenate is a critical step for its biotransformation into a form that can be transported by ARSENITE TRANSPORTING ATPASES or complexed by specific sulfhydryl-containing proteins for the purpose of detoxification (METABOLIC DETOXIFICATION, DRUG). Arsenate reductases require reducing equivalents such as GLUTAREDOXIN or AZURIN.Arsenicals: Inorganic or organic compounds that contain arsenic.Plasma Membrane Calcium-Transporting ATPases: Calcium-transporting ATPases found on the PLASMA MEMBRANE that catalyze the active transport of CALCIUM from the CYTOPLASM into the extracellular space. They play a role in maintaining a CALCIUM gradient across plasma membrane.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Aquaglyceroporins: A subgroup of aquaporins that transport WATER; GLYCEROL; and other small solutes across CELL MEMBRANES.Antimony Potassium Tartrate: A schistosomicide possibly useful against other parasites. It has irritant emetic properties and may cause lethal cardiac toxicity among other adverse effects.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cation Transport Proteins: Membrane proteins whose primary function is to facilitate the transport of positively charged molecules (cations) across a biological membrane.Alcaligenes faecalis: The type species of gram negative bacteria in the genus ALCALIGENES, found in soil. It is non-pathogenic, non-pigmented, and used for the production of amino acids.Gram-Negative Chemolithotrophic Bacteria: A large group of bacteria including those which oxidize ammonia or nitrite, metabolize sulfur and sulfur compounds, or deposit iron and/or manganese oxides.Ectothiorhodospiraceae: A family in the order Chromatiales, class GAMMAPROTEOBACTERIA. These are haloalkaliphilic, phototrophic bacteria that deposit elemental sulfur outside their cells.H(+)-K(+)-Exchanging ATPaseSodium-Potassium-Exchanging ATPase: An enzyme that catalyzes the active transport system of sodium and potassium ions across the cell wall. Sodium and potassium ions are closely coupled with membrane ATPase which undergoes phosphorylation and dephosphorylation, thereby providing energy for transport of these ions against concentration gradients.Archaeoglobus fulgidus: A species of extremely thermophilic, sulfur-reducing archaea. It grows at a maximum temperature of 95 degrees C. in marine or deep-sea geothermal areas.Vacuoles: Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Proton Pumps: Integral membrane proteins that transport protons across a membrane. This transport can be linked to the hydrolysis of ADENOSINE TRIPHOSPHATE. What is referred to as proton pump inhibitors frequently is about POTASSIUM HYDROGEN ATPASE.Pteris: A plant genus of the family PTERIDACEAE. Members contain entkaurane DITERPENES. The name is similar to bracken fern (PTERIDIUM).Sarcoplasmic Reticulum Calcium-Transporting ATPases: Calcium-transporting ATPases that catalyze the active transport of CALCIUM into the SARCOPLASMIC RETICULUM vesicles from the CYTOPLASM. They are primarily found in MUSCLE CELLS and play a role in the relaxation of MUSCLES.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Ca(2+) Mg(2+)-ATPaseArsenic Poisoning: Disorders associated with acute or chronic exposure to compounds containing ARSENIC (ARSENICALS) which may be fatal. Acute oral ingestion is associated with gastrointestinal symptoms and an encephalopathy which may manifest as SEIZURES, mental status changes, and COMA. Chronic exposure is associated with mucosal irritation, desquamating rash, myalgias, peripheral neuropathy, and white transverse (Mees) lines in the fingernails. (Adams et al., Principles of Neurology, 6th ed, p1212)Cadmium Chloride: A cadmium halide in the form of colorless crystals, soluble in water, methanol, and ethanol. It is used in photography, in dyeing, and calico printing, and as a solution to precipitate sulfides. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Kinetics: The rate dynamics in chemical or physical systems.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Ralstonia: A genus in the family BURKHOLDERIACEAE, comprised of many species. They are associated with a variety of infections including MENINGITIS; PERITONITIS; and URINARY TRACT INFECTIONS.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Vanadates: Oxyvanadium ions in various states of oxidation. They act primarily as ion transport inhibitors due to their inhibition of Na(+)-, K(+)-, and Ca(+)-ATPase transport systems. They also have insulin-like action, positive inotropic action on cardiac ventricular muscle, and other metabolic effects.Oxalobacteraceae: A family of gram-negative bacteria in the class BETAPROTEOBACTERIA. There are at least eight genera.Copper: A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Multienzyme Complexes: Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Alcohol Drinking: Behaviors associated with the ingesting of alcoholic beverages, including social drinking.Phospholipid Transfer Proteins: A ubiquitous family of proteins that transport PHOSPHOLIPIDS such as PHOSPHATIDYLINOSITOL and PHOSPHATIDYLCHOLINE between membranes. They play an important role in phospholipid metabolism during vesicular transport and SIGNAL TRANSDUCTION.Alcoholism: A primary, chronic disease with genetic, psychosocial, and environmental factors influencing its development and manifestations. The disease is often progressive and fatal. It is characterized by impaired control over drinking, preoccupation with the drug alcohol, use of alcohol despite adverse consequences, and distortions in thinking, most notably denial. Each of these symptoms may be continuous or periodic. (Morse & Flavin for the Joint Commission of the National Council on Alcoholism and Drug Dependence and the American Society of Addiction Medicine to Study the Definition and Criteria for the Diagnosis of Alcoholism: in JAMA 1992;268:1012-4)Ethanol: A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES.Carnitine Acyltransferases: Acyltransferases in the inner mitochondrial membrane that catalyze the reversible transfer of acyl groups from acyl-CoA to L-carnitine and thereby mediate the transport of activated fatty acids through that membrane. EC 2.3.1.Alcoholic Beverages: Drinkable liquids containing ETHANOL.Phospholipids: Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.

Expression cloning for arsenite-resistance resulted in isolation of tumor-suppressor fau cDNA: possible involvement of the ubiquitin system in arsenic carcinogenesis. (1/104)

Arsenic is a human carcinogen whose mechanism of action is unknown. Previously, this laboratory demonstrated that arsenite acts as a comutagen by interfering with DNA repair, although a specific DNA repair enzyme sensitive to arsenite has not been identified. A number of stable arsenite-sensitive and arsenite-resistant sublines of Chinese hamster V79 cells have now been isolated. In order to gain understanding of possible targets for arsenite's action, one arsenite-resistant subline, As/R28A, was chosen as a donor for a cDNA expression library. The library from arsenite-induced As/R28A cells was transfected into arsenite-sensitive As/S5 cells, and transfectants were selected for arsenite-resistance. Two cDNAs, asr1 and asr2, which confer arsenite resistance to arsenite-hypersensitive As/S5 cells as well as to wild-type cells, were isolated. asr1 shows almost complete homology with the rat fau gene, a tumor suppressor gene which contains a ubiquitin-like region fused to S30 ribosomal protein. Arsenite was previously shown to inhibit ubiquitin-dependent proteolysis. These results suggest that the tumor suppressor fau gene product or some other aspect of the ubiquitin system may be a target for arsenic toxicity and that disruption of the ubiquitin system may contribute to the genotoxicity and carcinogenicity of arsenite.  (+info)

Asp45 is a Mg2+ ligand in the ArsA ATPase. (2/104)

The ATPase activity of ArsA, the catalytic subunit of the plasmid-encoded, ATP-dependent extrusion pump for arsenicals and antimonials in Escherichia coli, is allosterically activated by arsenite or antimonite. Magnesium is essential for ATPase activity. To examine the role of Asp45, mutants were constructed in which Asp45 was changed to Glu, Asn, or Ala. Cells expressing these mutated arsA genes lost arsenite resistance to varying degrees. Purified D45A and D45N enzymes were inactive. The purified D45E enzyme exhibited approximately 5% of the wild type activity with about a 5-fold decrease in affinity for Mg2+. Intrinsic tryptophan fluorescence was used to probe Mg2+ binding. ArsA containing only Trp159 exhibited fluorescence enhancement upon the addition of MgATP, which was absent in D45N and D45A. As another measure of conformation, limited trypsin digestion was used to estimate the surface accessibility of residues in ArsA. ATP and Sb(III) synergistically protected wild type ArsA from trypsin digestion. Subsequent addition of Mg2+ increased trypsin sensitivity. D45N and D45A remained protected by ATP and Sb(III) but lost the Mg2+ effect. D45E exhibited an intermediate Mg2+ response. These results indicate that Asp45 is a Mg2+-responsive residue, consistent with its function as a Mg2+ ligand.  (+info)

The ATPase mechanism of ArsA, the catalytic subunit of the arsenite pump. (3/104)

The ArsA ATPase is the catalytic subunit of a novel arsenite pump, with two nucleotide-binding consensus sequences in the N- and C-terminal halves of the protein. The single tryptophan-containing Trp159 ArsA was used to elucidate the elementary steps of the ATPase mechanism by fluorescence stopped-flow experiments. The binding and hydrolysis of MgATP is a multistep process with a minimal kinetic mechanism (Mechanism 1). A notable feature of the reaction is that MgATP binding induces a slow transient increase in fluorescence of ArsA, which is independent of the ATP concentration, indicative of the build-up of a pre-steady state intermediate. This finding, coupled with a phosphate burst, implies that the steady-state intermediate builds up subsequent to product release. We propose that the rate-limiting step is an isomerization between different conformational forms of ArsA. kcat is faster than the phosphate burst, indicating that both nucleotide binding sites of ArsA are catalytic. Consistent with this interpretation, approximately 2 mol of phosphate are released per mole of ArsA during the phosphate burst.  (+info)

The anion-stimulated ATPase ArsA shows unisite and multisite catalytic activity. (4/104)

ArsA, an anion-stimulated ATPase, consists of two nucleotide binding domains, A1 in the N terminus and A2 in the C terminus of the protein, connected by a linker. The A1 domain contains a high affinity ATP binding site, whereas the A2 domain has low affinity and it requires the allosteric ligand antimonite for binding ATP. ArsA is known to form a UV-activated adduct with [alpha-(32)P]ATP in the linker region. This study shows that on addition of antimonite, much more adduct is formed. Characterization of the nature of the adduct suggests that it is between ArsA and ADP, instead of ATP, indicating that the adduct formation reflects hydrolysis of ATP. The present study also demonstrates that the A1 domain is capable of carrying out unisite catalysis in the absence of antimonite. On addition of antimonite, multisite catalysis involving both A1 and A2 sites occurs, resulting in a 40-fold increase in ATPase activity. Studies with mutant proteins suggest that the A2 site may be second in the sequence of events, so that its role in catalysis is dependent on a functional A1 site. It is also proposed that ArsA goes through an ATP-bound and an ADP-bound conformation, and the linker region, where ADP binds under both unisite and multisite catalytic conditions, may play an important role in the energy transduction process.  (+info)

Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance. (5/104)

Four Nudix hydrolase genes, ysa1 from Saccharomyces cerevisiae, orf209 from Escherichia coli, yqkg from Bacillus subtilis, and hi0398 from Hemophilus influenzae were amplified, cloned into an expression vector, and transformed into E. coli. The expressed proteins were purified and shown to belong to a subfamily of Nudix hydrolases active on ADP-ribose. Comparison with other members of the subfamily revealed a conserved proline 16 amino acid residues downstream of the Nudix box, common to all of the ADP-ribose pyrophosphatase subfamily. In this same region, a conserved tyrosine designates another subfamily, the diadenosine polyphosphate pyrophosphatases, while an array of eight conserved amino acids is indicative of the NADH pyrophosphatases. On the basis of these classifications, the trgB gene, a tellurite resistance factor from Rhodobacter sphaeroides, was predicted to designate an ADP-ribose pyrophosphatase. In support of this hypothesis, a highly specific ADP-ribose pyrophosphatase gene from the archaebacterium, Methanococcus jannaschii, introduced into E. coli, increased the transformant's tolerance to potassium tellurite.  (+info)

Mechanism of the ArsA ATPase. (6/104)

The ArsAB ATPase confers metalloid resistance in Escherichia coli by pumping toxic anions out of the cells. This transport ATPase shares structural and perhaps mechanism features with ABC transporters. The ArsAB pump is composed of a membrane subunit that has two groups of six transmembrane segments, and the catalytic subunit, the ArsA ATPase. As is the case with many ABC transporters, ArsA has an internal repeat, each with an ATP binding domain, and is allosterically activated by substrates of the pump. The mechanism of allosteric activation of the ArsA ATPase has been elucidated at the molecular level. Binding of the activator produces a conformational change that forms a tight interface of the nucleotide binding domains. In the rate-limiting step in the overall reaction, the enzyme undergoes a slow conformational change. The allosteric activator accelerates catalysis by increasing the velocity of this rate-limiting step. We postulate that similar conformational changes may be rate-limiting in the mechanism of ABC transporters.  (+info)

Trimeric ring-like structure of ArsA ATPase. (7/104)

ArsA protein is the soluble subunit of the Ars anion pump in the Escherichia coli membrane which extrudes arsenite or antimonite from the cytoplasm. The molecular weight of the subunit is 63 kDa. In the cell it hydrolyzes ATP, and the energy released is used by the membrane-bound subunit ArsB to transport the substrates across the membrane. We have obtained two-dimensional crystals of ArsA in the presence of arsenite on negatively-charged lipid monolayer composed of DMPS and DOPC. These crystals have been studied using electron microscopy of negatively-stained specimens followed by image processing. The projection map obtained at 2.4 nm resolution reveals a ring-like structure with threefold symmetry. Many molecular assemblies with the same ring-shape and dimensions were also seen dispersed on electron microscopy grids, prepared directly from purified ArsA protein solution. Size-exclusion chromatography of the protein sample with arsenite present revealed that the majority of the protein particles in solution have a molecular weight of about 180 kDa. Based on these experiments, we conclude that in solution the ArsA ATPase with substrate bound is mainly in a trimeric form.  (+info)

The chromosomal arsenic resistance genes of Thiobacillus ferrooxidans have an unusual arrangement and confer increased arsenic and antimony resistance to Escherichia coli. (8/104)

The chromosomal arsenic resistance genes of the acidophilic, chemolithoautotrophic, biomining bacterium Thiobacillus ferrooxidans were cloned and sequenced. Homologues of four arsenic resistance genes, arsB, arsC, arsH, and a putative arsR gene, were identified. The T. ferrooxidans arsB (arsenite export) and arsC (arsenate reductase) gene products were functional when they were cloned in an Escherichia coli ars deletion mutant and conferred increased resistance to arsenite, arsenate, and antimony. Therefore, despite the fact that the ars genes originated from an obligately acidophilic bacterium, they were functional in E. coli. Although T. ferrooxidans is gram negative, its ArsC was more closely related to the ArsC molecules of gram-positive bacteria. Furthermore, a functional trxA (thioredoxin) gene was required for ArsC-mediated arsenate resistance in E. coli; this finding confirmed the gram-positive ArsC-like status of this resistance and indicated that the division of ArsC molecules based on Gram staining results is artificial. Although arsH was expressed in an E. coli-derived in vitro transcription-translation system, ArsH was not required for and did not enhance arsenic resistance in E. coli. The T. ferrooxidans ars genes were arranged in an unusual manner, and the putative arsR and arsC genes and the arsBH genes were translated in opposite directions. This divergent orientation was conserved in the four T. ferrooxidans strains investigated.  (+info)

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
In molecular biology, the ars operon is an operon found in several bacterial taxon. It is required for the detoxification of arsenate, arsenite, and antimonite. This system transports arsenite and antimonite out of the cell. The pump is composed of two polypeptides, the products of the arsA and arsB genes. This two-subunit enzyme produces resistance to arsenite and antimonite. Arsenate, however, must first be reduced to arsenite before it is extruded. A third gene, arsC, expands the substrate specificity to allow for arsenate pumping and resistance. ArsC is an approximately 150-residue arsenate reductase that uses reduced glutathione (GSH) to convert arsenate to arsenite with a redox active cysteine residue in the active site. ArsC forms an active quaternary complex with GSH, arsenate, and glutaredoxin 1 (Grx1). The three ligands must be present simultaneously for reduction to occur. ArsA and ArsB form an anion-translocating ATPase. The ArsB protein is distinguished by its overall hydrophobic ...
This study shows a critical role for Asna1 in ensuring β-cell function. Loss of Asna1 in β-cells of mice results in pancreatic hypoinsulinemia, impaired insulin secretion, and early onset diabetes. Additionally, β-cells of Asna1β−/− mice showed impaired PM-to-TGN as well as Golgi-to-ER retrograde transport, ER stress, and mislocalization of Stx5 and Stx6. Of note, we also show that inhibition of retrograde transport at the level of EE-to-TGN in isolated islet and insulinoma cells results in impaired Golgi-to-ER retrograde transport, decreased insulin content, and ER stress. Thus, the findings provide evidence that Asna1 is required in β-cells to ensure retrograde transport, which in turn appears to be essential for ER homeostasis and proinsulin biogenesis. Additionally, the perturbed Golgi-to-ER retrograde transport in Retro-2-treated primary islets suggests that the impairment of this step in Asna1β−/− β-cells likely is secondary to the inhibition of retrograde transport at the ...
Słowa kluczowe: leczenie przewlekłych zapaleń tkanek okołowierzchołkowych, preparaty wodorotlenkowapniowe. W leczeniu przewlekłych zapaleń tkanek okołowierzchołkowych istotne jest działanie bakteriobójcze oraz stworzenie warunków do reparacji uszkodzonej kości. Najbardziej skuteczną metodą terapeutyczną okazało się biomechaniczne opracowanie kanału oraz zastosowanie wkładki leczniczej z wodorotlenku wapnia. Celem pracy była własna ocena wpływu nie twardniejących preparatów wodorotlenkowapniowych (Calxyl, Biopulp) na procesy gojenia i odnowy kości w przewlekłych zapaleniach przyzębia przyszczytowego. Leczeniem objęto 96 zębów jedno- i wielokorzeniowych. Na podstawie diagnostycznych zdjęć rtg ustalono średnicę zmian zapalnych. Wyodrębniono ogniska o średnicy do 3 mm, od 3 do 6 mm i powyżej 6 mm. Wkładki lecznicze z preparatów wodorotlenkowapniowych zmieniano co 3 miesiące. Obserwacje radiologiczne przeprowadzono po 3, 6, 9 i 12 miesiącach. Ogółem na 96 ...
Pogosti stranski učinki so povezani z antiholinergičnim delovanjem na parasimpatične postsinaptične receptorje: suha usta, grlo, nos, ob prekomernih odmerkih oslabljen govor, ki se lahko stopnjuje do mrmranja, podobnega glasovom rakuna; žeja, zamegljen vid in občutljivost na svetlobo, zaprtje, težave pri uriniranju in tahikardija. Ostali stranski učinki: pordelost, povišana temperatura, razburjenje, nemirnost, halucinacije, delirij, sploh pri višjih odmerkih. Našteti stranski učinki nastopijo ob peroralni ali parenteralni aplikaciji učinkovine, ne pa ob topični ali okularni uporabi. Ekstremni zaplet ob zaužitju zelo visokih odmerkov skopolamina in sorodnih drog je začasna slepota, ki lahko traja do 72 ur.. Vročina in izsušitev zaradi skopolamina lahko prisilita uporabnike tega mamila, da se poskušajo ohladiti v različnih vodnih zajetjih in se tam utopijo.. Stranski učinki skopolamina se lahko zamenjajo za simptome raka zaradi prisotne slabosti in različno velikih zenic ...
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Plasmid pIS1 mINSIG2 from Dr. David Bartels lab contains the insert INSIG2 3UTR and mutated miR-155 binding site and is published in Mol Cell. 2014 Mar 20;53(6):1031-43. doi: 10.1016/j.molcel.2014.02.013. Epub 2014 Mar 13. This plasmid is available through Addgene.
Define antimonite. antimonite synonyms, antimonite pronunciation, antimonite translation, English dictionary definition of antimonite. n an oxyanion of antimony or a salt containing an oxyanion of antimonya mineral from which antimony is obtained
The ArsA ATPase is the catalytic subunit of the pump protein, coupling the hydrolysis of ATP to the movement of arsenicals and antimonials through the membrane-spanning ArsB protein. Previously, we have shown the binding and hydrolysis of MgATP to ArsA to be a multi-step process in which the rate-limiting step is an isomerization between different conformational forms of ArsA. This isomerization occurs after product release, at the end of the ATPase reaction, and involves the return of the ArsA to its original conformation, which can then bind MgATP. ArsA possesses an allosteric site for antimonite [Sb(III)], the binding of which elevates the steady-state ATPase activity. We have used a transient kinetics approach to investigate the kinetics of ternary complex formation that lead to an enhancement in the ATPase activity. These studies revealed that ArsA exists in at least two conformational forms that differ in their ligand binding affinities, and that ATP favours one form and Sb(III) the other. ...
Complete information for ARSA gene (Protein Coding), Arylsulfatase A, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ARSA gene (Protein Coding), Arylsulfatase A, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Gentaur molecular products has all kinds of products like :search , Zyagen \ ASNA1 Mouse Monoclonal Antibody \ APO-000439-M03 for more molecular products just contact us
Company Name: American Security Resources Corp., Stock Symbol: ARSC, Industry: Alternative Energy, Total Posts: 51817, Last Post: 1/13/2018 1:39:05 PM
Razmnoževanje bakterij poteka v treh fazah. Kadar populacija bakterij vstopi v zelo hranljivo okolje, ki omogoča rast, se morajo celice najprej prilagoditi novemu okolju. V prvi fazi razvoja je značilna počasna rast, kjer se celice najprej prilagajajo in pripravljajo za hitro rast. Naslednji korak je logaritmična faza oziroma eksponentna rast, kar pomeni, da se bakterije, če njihovo število merimo po enakomernem časovnem intervalu, začnejo razmnoževati z enakim faktorjem oziroma količnikom, ki ga glede na število intervalov potenciramo. To se dogaja vse dokler se hranljive snovi ne izrabijo. Po tej fazi nastopi zadnja faza, ki ji rečemo faza mirovanja, kjer se bakterije ne razmnožujejo več. ...
Najem samochodów dostawczych w Warszawie jakkolwiek usługa idealnie skrojona na ilość potrzeb współczesnego Konsumenta! Stolica jest stroną, w jakiej idzie relatywnie sporo wypożyczalni wypożyczalnia aut dostawczych warszawa (click https://hellogoodbyethankyou.tumblr.com/), natomiast ich mężczyznami są nie tylko przybywający tu w handlach biznesmeni, jakkolwiek dodatkowo zwykli obywatele Najważniejszym mieście, jacy wymagają samochodu naprawdę rzadko, iż nie finansuje im się umieć go na cecha - po prostu w razie sprawy go wynajmują. Jesteśmy skupieni na nowoczesnym, aby dać Państwu kompletne szczęście - niezależnie od aktualnego, lub potrzebują Państwo zdecydować się na wynajem krótkoterminowy, średnioterminowy albo i długoterminowy, własna oferta zawsze stanowi efektywna a będzie ekskluzywnym wsparciem niezależnie z robionej poprzez Państwa działalności. Interes prowadzi także możliwość rezerwacji telefonicznej, i swej w lokalnej wypożyczalni ...
대구광역시 동구 동내로 64 (동내동 1119) KERIS빌딩 우)41061 고객센터 (평일 09:00~18:00) 1599-3122 [ARS 번호 안내] ...
대구광역시 동구 동내로 64 (동내동 1119) KERIS빌딩 우)41061 고객센터 (평일 09:00~18:00) 1599-3122 [ARS 번호 안내] ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Arsenic atom in PDB 1j9b: Arsenate Reductase+0.4M Arsenite From E. Coli
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Goat polyclonal antibody raised against synthetic peptide of ARSB. A synthetic peptide corresponding to human ARSB. (PAB7369) - Products - Abnova
Arsa - mouse gene knockout kit via CRISPR, 1 kit. |dl||dt|Kit Component:|/dt||dd|- |strong|KN301623G1|/strong|, Arsa gRNA vector 1 in |a href=http://www.origene.com/CRISPR-CAS9/Detail.
ARSB Antibody 13227-1-AP has been identified with ELISA, WB, IHC. 13227-1-AP detected 44kd band in HepG2 cells with 1:500-1:1000 dilution...
Human ARSA partial ORF ( AAH14210, 398 a.a. - 507 a.a.) recombinant protein with GST-tag at N-terminal. (H00000410-Q01) - Products - Abnova
When relubricating bearings from the side in housings with V-ring seals, mount an additional V-ring inside the housing on the side where grease is applied (fig. 5). This forces the grease to travel through the bearing and exit the housing on the opposite side.. SKF can supply an appropriate V-ring together with a splash plate that fits in the seal groove to cover a bit more than the top half of the housing. The sets are identified by the series designation ASNA followed by the housing size identification and the suffix V, e.g. ASNA 3024 V, and are available from size 3024 to 3032.. ...
When relubricating bearings from the side in housings with V-ring seals, mount an additional V-ring inside the housing on the side where grease is applied (fig. 5). This forces the grease to travel through the bearing and exit the housing on the opposite side.. SKF can supply an appropriate V-ring together with a splash plate that fits in the seal groove to cover a bit more than the top half of the housing. The sets are identified by the series designation ASNA followed by the housing size identification and the suffix V, e.g. ASNA 3024 V, and are available from size 3024 to 3032.. ...
This is your chance to make annotations OR challenge other teams annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored. ...
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Glucoprivation activates neurons in the perifornical hypothalamus (PeH) and in the rostral ventrolateral medulla (RVLM), which results in release of adrenaline. The current study aimed to establish (i) whether neuroglucoprivation in the PeH or in the RVLM elicits adrenaline release in vivo; and (ii) whether direct activation by glucoprivation or orexin release in the RVLM modulates the adrenaline release. Neuroglucoprivation in the PeH or RVLM was elicited by microinjections of 2-deoxy-D-glucose or 5-thio-D-glucose in anesthetized, euglycemic, rats. We found that inhibition of neurons in the PeH abolished the increase in adrenal sympathetic nerve activity (ASNA) to systemic glucoprivation. Secondly, glucoprivation of neurons in the PeH increased ASNA. Thirdly, in vivo or in vitro glucoprivation did not affect the activity of RVLM adrenal premotor neurons. Finally, blockade of orexin receptors in the RVLM abolished the increase in ASNA to neuroglucoprivation in the PeH. The evoked changes in ASNA ...
As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20676083, PubMed:28104892). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome (PubMed:28104892). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the
Villadangos, A. F., K. Van Belle, K. Wahni, V. Tamu Dufe, S. Freitas, H. Nur, S. De Galan, J. A. Gil, J-F. Collet, L. M. Mateos, et al., Corynebacterium glutamicum survives arsenic stress with arsenate reductases coupled to two distinct redox mechanisms., Mol Microbiol, vol. 82, issue 4, pp. 998-1014, 2011 Nov. ...
Villadangos, A. F., K. Van Belle, K. Wahni, V. Tamu Dufe, S. Freitas, H. Nur, S. De Galan, J. A. Gil, J-F. Collet, L. M. Mateos, et al., Corynebacterium glutamicum survives arsenic stress with arsenate reductases coupled to two distinct redox mechanisms., Mol Microbiol, vol. 82, issue 4, pp. 998-1014, 2011 Nov. ...
Friemel, Thomas N. (2008): Hierarchical Structures in Digraphs - Measurement and Interpretation. In: Serdült, Uwe/Täube, Volker G. (Eds.): Applications of Social Network Analysis ASNA 2005. Berlin: Wissenschaftlicher Verlag, p. 341-356. ISBN 978-3-86573-374-0 ...
Plasmid XNOR-gate-V2.0-ASV from Dr. Drew Endys lab contains the insert XNOR-gate and is published in Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28. This plasmid is available through Addgene.
1JL3: Bacillus subtilis arsenate reductase is structurally and functionally similar to low molecular weight protein tyrosine phosphatases.
WRB Antibody (monoclonal) (M04), Mouse monoclonal antibody raised against a partial recombinant WRB. validated in WB, E (AT4542a), Abgent
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Business Directory for Ammonium Arsenate Suppliers in Mumbai - Get contact details of Ammonium Arsenate Manufacturers, Wholesale Ammonium Arsenate Exporters, Best Ammonium Arsenate Traders & Distributors Across the Mumbai.
Arsenate minerals usually refer to the naturally occurring orthoarsenates, possessing the (AsO4)3− anion group and, more rarely, other arsenates with anions like AsO3(OH)2− (also written HAsO42−) (example: pharmacolite Ca(AsO3OH).2H2O) or (very rarely) [AsO2(OH)2]− (example: andyrobertsite). Arsenite minerals are much less common.[1] Both the Dana[2] and the Strunz[3] mineral classifications place the arsenates in with the phosphate minerals. Example arsenate minerals include: ...
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One of the guys remarked about the wedding..."there goes the first one down!"...BUT...this is a tight group of friends and that the old song lyric ("wedding bells are breaking up that old gang of mine") will probably NOT apply. ...
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ARSB : Arylsulfatase B (ARSB) can be measured with nitrocatechol sulfate as the substrate. The conditions established are such that arylsulfatase A activity is minimal and its residual activity can be accounted for and subtracted from the activity of ARSB.(Baum H, Dodgson KS, Spencer B: Assay of arylsulfatases A and B in human urine. Clin Chim Acta 1959;4:453-455)
Proc Natl Acad Sci U S A. 2013 May 7;110(19):7666-71. doi: 10.1073/pnas.1222054110. Epub 2013 Apr 22. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt; Research Support, U.S. Govt, Non-P.H.S.
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I just stumbled across a new blog on Geology that looks quite promising. The blog is called Antimonite. Who writes it? The author of this blog is a Swedish student of geology at the department of Geoscience at the University of Lund. I have studied a great deal before going for geology. Basically lurking around…
19.3 Wages and salaries comprise the value of employee entitlements, including those paid in cash and in kind, and changes in provisions for future employee entitlements (although the latter component has not yet been implemented in the ASNA). Cash incomes are gross payments before taxation and other deductions, and include commissions, tips, sick pay, pay for annual and long service leave, penalty pay and shift allowances. Income in kind covers the cost to the employer of goods and services which are provided to the employee, or to another member of the employees household, free of charge or at a substantial discount, and which are clearly of benefit to the employee as a consumer. Examples are the provision of food and accommodation (other than when the employee is travelling on business for the employer), motor vehicles for private use, and education expenses. Loans by employers to employees at rates of interest below market rates are regarded as involving income in kind. Consequently, the ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
By the end of this section, you will be able to: Describe the general preparation, properties, and uses of the metalloids Describe the preparation, pr
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This picture was taken by me a long time ago (6 years, i think). Contrary to the name of the blog, this is not GFP. To take this picture, I performed Immunofluorescence experiment. The procedure is as follows: Cells are treated with whatever you are invetigating (in this case - cells were treated with 0.5mM arsenite…
Arsenic is a toxic metalloid which is widely distributed in nature. It is normally present as arsenate under oxic conditions while arsenite is predominant under reducing condition. The major discharges of arsenic in the environment are mainly due to natural sources such as aquifers and anthropogenic sources. It is known that arsenite salts are more toxic than arsenate as it binds with vicinal thiols in pyruvate dehydrogenase while arsenate inhibits the oxidative phosphorylation process. The common mechanisms for arsenic detoxification are uptaken by phosphate transporters, aquaglyceroporins, and active extrusion system and reduced by arsenate reductases via dissimilatory reduction mechanism. Some species of autotrophic and heterotrophic microorganisms use arsenic oxyanions for their regeneration of energy. Certain species of microorganisms are able to use arsenate as their nutrient in respiratory process. Detoxification operons are a common form of arsenic resistance in microorganisms. Hence, the use of
Theres an article from 2004 about a vet who treated falcons diagnosed with CFS with Potassium Arsenite 0.05% leading to complete remission of symptoms...
Okay so I noticed my lack of including info has sparked my providing additional information regarding ASV support. I have a resmed aircurve 10 ASV and having a little difficulty getting use to it. Its
... superfamily ARC3 family Arsenite-Antimonite efflux Arsenite-transporting ATPase Solute carrier family Active transport ATP- ... Arsenite or Antimonite (out). The overall reaction catalyzed by ArsB-ArsA is: Arsenite or Antimonite (in) + ATP ⇌ Arsenite or ... Rensing, C.; Ghosh, M.; Rosen, B. P. (1999-10-01). "Families of soft-metal-ion-transporting ATPases". Journal of Bacteriology. ... Arsenite resistance (Ars) efflux pumps of bacteria may consist of two proteins, ArsB (TC# 2.A.45.1.1; the integral membrane ...
It is required for the detoxification of arsenate, arsenite, and antimonite. This system transports arsenite and antimonite out ... thus increasing its ATPase activity at lower concentrations of arsenite and enhancing the rate of arsenite extrusion. Carlin A ... This two-subunit enzyme produces resistance to arsenite and antimonite. Arsenate, however, must first be reduced to arsenite ... ArsA and ArsB form an anion-translocating ATPase. The ArsB protein is distinguished by its overall hydrophobic character, in ...
Arsenic toxicity Arsenite-transporting ATPase Solute carrier family Active transport ATP-binding cassette transporter Hasgekar ... Arsenite-antimonite transporters are membrane transporters that pump arsenite or antimonite out of a cell. Antimonite is the ... an ATPase, must be superimposed onto ArsB. Arsenite and antimonite can also be pumped out of the cell by members of the ARC3 ... which can participate in both secondary transport or primary active transport. Based on operon analyses, Arc3 homologues may ...
In enzymology, an arsenite-transporting ATPase (EC 3.6.3.16) is an enzyme that catalyzes the chemical reaction ATP + H2O + ... and arsenite, whereas its 3 products are ADP, phosphate, and arsenite. This enzyme belongs to the family of hydrolases, ... Arsenite-Antimonite efflux Silver S, Misra TK, Laddaga RA (1989). "DNA sequence analysis of bacterial toxic heavy metal ... The systematic name of this enzyme class is ATP phosphohydrolase (arsenite-exporting). As of late 2007, 3 structures have been ...
V-type ATPase and A-type ATPase superfamily 3.A.3 The P-type ATPase Superfamily 3.A.4 The Arsenite-Antimonite efflux family 3.A ... Family 3.B.1 The Na+-transporting Carboxylic Acid Decarboxylase (NaT-DC) Family 3.C.1 The Na+ Transporting ... Family 1.D.58 The Anion Transporting Prodigiosene (Prodigiosene) Family 1.D.59 The Anion Transporting Perenosin (Perenosin) ... See electron transport chain. 3.D.1 The H+ or Na+-translocating NADH Dehydrogenase ("complex I") family 3.D.2 The Proton- ...
The Bacillus protein exports both arsenite and antimonite. The exact transport mechanism has not established. The generalized ... Homologous ATPases are found in families TC# 3.A.4, TC# 3.A.19 and TC# 3.A.21 as well as TC# 2.A.59. A region of the ABC ATPase ... In the latter case ATP hydrolysis again energizes transport. ARC3 homologues transport the same anions as ArsA/AB homologues, ... reaction catalyzed by members of the ACR3 family is: arsenite or antimonite (in) → arsenite or antimonite (out). Arsenite- ...
An underlying mechanism by which lead is able to cause harm is its ability to be transported by calcium ATPase pumps across the ... As a metabolite of arsenic, arsenite is formed after ingestion of arsenic and has shown significant toxicity to neurons within ... This is important as neurotransmitter transport can be impaired through vesicular transport inhibition, resulting in diminished ... This barrier creates a tight hydrophobic layer around the capillaries in the brain, inhibiting the transport of large or ...
H+-transporting two-sector ATPase EC 3.6.3.15: Na+-transporting two-sector ATPase EC 3.6.3.16: arsenite-transporting ATPase EC ... teichoic-acid-transporting ATPase EC 3.6.3.41: heme-transporting ATPase EC 3.6.3.42: beta-glucan-transporting ATPase EC 3.6. ... steroid-transporting ATPase EC 3.6.3.46: cadmium-transporting ATPase EC 3.6.3.47: fatty-acyl-CoA-transporting ATPase EC 3.6. ... vitamin B12-transporting ATPase EC 3.6.3.34: iron-chelate-transporting ATPase EC 3.6.3.35: manganese-transporting ATPase EC 3.6 ...
Protons are transported across the membrane by the initial NADH reductase, quinones, and nitrous oxide reductase to produce the ... In all cases, however, a proton motive force is generated and used to drive ATP production via an ATPase. Most photosynthetic ... reduction to arsenite (AsO3− 3) Uranyl ion ion (UO2+ 2) reduction to uranium dioxide (UO 2) A number of organisms, instead of ... This means that these organisms do not use an electron transport chain to oxidize NADH to NAD+ and therefore must have an ...
It has also been proposed that microtubules play a role in the formation of stress granules, maybe by transporting granule ... Jain S, Wheeler JR, Walters RW, Agrawal A, Barsic A, Parker R (January 2016). "ATPase-Modulated Stress Granules Contain a ... Chang YW, Huang YS (2014). "Arsenite-activated JNK signaling enhances CPEB4-Vinexin interaction to facilitate stress granule ... In vitro, these stressors can include heat, cold, oxidative stress (sodium arsenite), endoplasmic reticulum stress ( ...
... metal transporting P1-type ATPases and a chemiosmotic antiporter efflux system similar to CzcCBA of Cupriavidus metallidurans. ... MM-1 isolated from a soil and identification of arsenite oxidase gene". Journal of Hazardous Materials. 262: 997-1003. doi: ... Bahar, Md Mezbaul; Megharaj, Mallavarapu; Naidu, Ravi (2013-11-15). "Kinetics of arsenite oxidation by Variovorax sp. ...
In enzymology, an arsenite-transporting ATPase (EC 3.6.3.16) is an enzyme that catalyzes the chemical reaction ATP + H2O + ... and arsenite, whereas its 3 products are ADP, phosphate, and arsenite. This enzyme belongs to the family of hydrolases, ... Arsenite-Antimonite efflux Silver S, Misra TK, Laddaga RA (1989). "DNA sequence analysis of bacterial toxic heavy metal ... The systematic name of this enzyme class is ATP phosphohydrolase (arsenite-exporting). As of late 2007, 3 structures have been ...
... a member of the ABC superfamily of transport ATPases. Arsenate is reduced to arsenite via the ArsC arsenate reductase. Arsenite ... They have previously been described as CPx-type ATPases (55) or soft-metal-transporting P-type ATPases (42). These ATPases have ... SOFT-METAL-TRANSPORTING P-TYPE ATPASES. The superfamily of P-type ATPases is a ubiquitous group of proteins involved in ... Soft-metal-transporting P-type ATPases can be further divided into subgroups that contain Cu(I)/Ag(I)-translocating ATPases and ...
ATPase II (ATP8A1) can display an ATP-dependent phospholipid translocase activity. However, the function of ATP8A1 in ... Keywords: Apoptosis, Arsenite Transporting ATPases, Hepatocytes, phosphatidylinositol 3-kinase. Full Text Order reprints Export ... Over-Expression of ATPase II Alleviates Ethanol-Induced Hepatocyte Injury in HL-7702 Cells. Qing Li, Yan Yang, Ying Liu ... ATPase II (ATP8A1) can display an ATP-dependent phospholipid translocase activity. However, the function of ATP8A1 in ...
Anion-transporting ATPase. Catalyzes the extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low ... Miscellaneous arsenites. Alternative Parents. Trivalent inorganic arsenic compounds / Metalloid salts / Inorganic salts. ... Arsenite / Trivalent inorganic arsenic compound / Inorganic salt / Inorganic metalloid salt / Inorganic arsenic compound. ... These are inorganic compounds in which the largest metallic oxoanion is arsenite, to which either no atom or a non metal atom ...
Once absorbed, inorganic arsenic is transported to the liver, where it is reduced to arsenite and then methylated. Most ... Vanadium also stimulates cell proliferation and differentiation and inhibits various ATPases, phosphatases, and phosphoryl- ... Nickel is transported through the blood bound primarily to albumin. Most organs and tissues do not accumulate nickel, but in ... ganic forms that contain trivalent arsenite (III) or pentavalent arsenate (V) hav- ing the greatest toxicological significance ...
Arsenic toxicity Arsenite-transporting ATPase Solute carrier family Active transport ATP-binding cassette transporter Hasgekar ... Arsenite-antimonite transporters are membrane transporters that pump arsenite or antimonite out of a cell. Antimonite is the ... an ATPase, must be superimposed onto ArsB. Arsenite and antimonite can also be pumped out of the cell by members of the ARC3 ... which can participate in both secondary transport or primary active transport. Based on operon analyses, Arc3 homologues may ...
The arsenic resistance efflux system transports arsenite, using alternatively either a two-component (ArsA and ArsB) ATPase or ... encode P-type ATPases that are more similar to the bacterial CadA and CopB ATPases than to eukaryote ATPases that pump ... Other systems, such as copper transport ATPases and metallothionein cation-binding proteins, are only known from chromosomal ... The CadA ATPase protein has been labeled with 32P from gamma-32P-ATP and drives ATP-dependent Cd2+ uptake by inside-out ...
The recent clusters include K+ channel proteins (285 members), ABC transporters (152 members), cation-transporting ATPases (59 ... arsenite, and chromate ion were identified, and there are no predicted anion channels. The reason for the difference in the ... and phospholipid-transporting ATPases (20 members). The expansion of the K+ channel proteins, which are VIC-type transporters, ... Most of these are channel-type transporters and cation-transporting P-type ATPases. Interestingly, despite the apparent massive ...
It is known that arsenite salts are more toxic than arsenate as it binds with vicinal thiols in pyruvate dehydrogenase while ... It is normally present as arsenate under oxic conditions while arsenite is predominant under reducing condition. The major ... arsenate that enters the cell will be reduced to arsenite by ArsC gene before it is transported out of the cell by ArsB gene [ ... translocating ATPase, an ATP-driven efflux pump) [3, 53, 64]. Moreover, some detoxification operons also contain two additional ...
... membrane-associated arsenite permeases (ArsB or ACR3), and arsenite-translocating ATPases (ArsA). Other genes for arsenate ... Heavy Metal Transport and Detoxification. A. richmondensis encoded and expressed many genes related to heavy metal transport ... Overall, 24 metal transport and detoxification proteins were measured only in streamer biofilms, and an additional eight ... However, A. richmondensis genes involved in responding to these harsh conditions (i.e., metal transport and detoxification and ...
Arsenite-transporting ATPase 3.6.3.17 Monosaccharide-transporting ATPase 3.6.3.18 Transferred entry: 7.5.2.2 3.6.3.19 ... Chloride-transporting ATPase 3.6.3.12 Transferred entry: 7.2.2.6 3.6.3.13 Transferred entry: 3.6.3.1 3.6.3.14 Transferred entry ... Vesicle-fusing ATPase 3.6.4.7 Peroxisome-assembly ATPase 3.6.4.8 Transferred entry: 5.6.1.5 3.6.4.9 Transferred entry: 5.6.1.7 ... Cadmium-exporting ATPase 3.6.3.4 Transferred entry: 7.2.2.9 3.6.3.5 Transferred entry: 7.2.2.12 3.6.3.6 Transferred entry: 7.1. ...
... an ATPase that associates with ArsB and links arsenite export to ATP hydrolysis)22. The highest sequence identities obtained ... 11 Arsenate entering the cell is reduced to the more toxic arsenite by ArsC, and is then transported out of the cell via ArsB ( ... Ji G, Silver S. Reduction of arsenate to arsenite by the ArsC protein of the arsenic resistance operon of Staphylococcus aureus ... Sato T, Kobayashi Y. The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite. J ...
Arsenite Transporting ATPases * Biochemistry & Molecular Biology * Cell Membrane * Drug Resistance, Microbial * Enzyme ... Interaction of the catalytic and the membrane subunits of an oxyanion-translocating ATPase. Academic Article * Authors Version ... Addition of ATP had little affect on membrane binding of the ArsA ATPase subunit. In the presence or absence of the anionic ... In the absence of arsenite or antimonite an apparent positive cooperativity in the binding of the ArsA protein to membrane ...
Arsenite Transporting ATPases/chemistry. *Arsenite Transporting ATPases/genetics. *Arsenite Transporting ATPases/metabolism* ... The highly conserved, 40 kDa ATPase subunit of TRC (which we termed TRC40) was identified as Asna-1. TRC40/Asna-1 interacts ... Consequently, an ATPase-deficient mutant of TRC40/Asna-1 dominantly inhibited TA protein insertion selectively without ...
Hypothetical protein; Anion-transporting ATPase. Catalyzes the extrusion of arsenite (By similarity) (396 aa) ... Hypothetical protein; Anion-transporting ATPase. Catalyzes the extrusion of arsenite (By similarity) ... Arsenate reductase; Reduces arsenate [As(V)] to arsenite [As(III)] using glutathione and glutaredoxin as sources of reducing ... Arsenate reductase; Reduces arsenate [As(V)] to arsenite [As(III)] using glutathione and glutaredoxin as sources of reducing ...
anion transport. go/ biological_process. arsenite-transmembrane transporting ATPase activity. go/ molecular_function. membrane ... Oxyanion-translocating ATPase. cog/ cog. ATP binding. go/ molecular_function. ... anion transport. go/ biological_process. arsenite-transmembrane transporting ATPase activity. go/ molecular_function. ...
3.A.3 The P-type ATPase (P-ATPase) Superfamily. 3.A.4 The Arsenite-Antimonite (ArsAB) Efflux Family ... Transport systems are included in this subclass if they hydrolyze the diphosphate bond of inorganic pyrophosphate, ATP, or ... 3.A.2 The H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) Superfamily ... The transport protein may or may not be transiently phosphorylated, but the substrate is not phosphorylated. ...
Arsenite Transporting ATPases. *Ca(2+) Mg(2+)-ATPase. *Calcium-Transporting ATPases. *Chaperonins ... The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes ... Pathogenic mutations in the ALS gene CCNF cause cytoplasmic mislocalization of Cyclin F and elevated VCP ATPase activity. Hum ... Cycle Restores Wild-Type Function in the Lipopolysaccharide Transporter and Shows that ATP Binding Triggers Transport. mBio. ...
Arsenite-transporting ATPase. *Arsole. *Arthropod. *Artificial respiration. *Asbestos. *Ascophyllum nodosum. *Ashermans ...
ATP-dependent arsenite transport in everted membrane vesicles of Escherichia coli[J].J Biol Chem, 1994, 269(4): 25442-25446. . ... ArsD: an As(Ⅲ) metallochaperone for the ArsAB As(Ⅲ)-translocating ATPase[J].J Bioenerg Biomembr, 2007, 39(5-6): 453-458. . ... Analysis of the capability of ultra-highly diluted glucose to increase glucose uptake in arsenite-stressed bacteria Escherichia ... Results: In arsenite-exposed E. coli, the glucose uptake increased along with decreases in the specific activities of ...
claimed that the arsenite binding site of the E. coli ArsA is not conserved in Arr4p. The homodimer binds to the C-terminus of ... In yeast, it has been shown that the adenosine triphosphatase (ATPase) Get3 (guided-entry of TA proteins-3 pathway) is ... GET3 (Arr4) thus inhibits Cl- transport via Gef1p (TC #1.A.11.1.1). ... It may also be involved in low-level resistance to oxyanions such as arsenite, and in heat tolerance (Shen et al., 2003). It ...
ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and ... Arsenite-stimulated ATPaseUniRule annotation. Manual assertion according to rulesi. *HAMAP-Rule:MF_03112 ... retrograde vesicle-mediated transport, Golgi to ER Source: EnsemblFungi. View the complete GO annotation on QuickGO ... ... IPR025723 Anion-transp_ATPase-like_dom. IPR016300 ATPase_ArsA/GET3. IPR027542 ATPase_ArsA/GET3_euk. IPR027417 P-loop_NTPase. ...
Arsenite Transporting ATPases. *Asparagine. *Autophagy. *Bacteria. *Bacterial Proteins. *Base Sequence. *Basic Helix-Loop-Helix ...
... arsenite (ArsAB, ACR3) and other metal ions (ABC and P-ATPase) and 1 each for nickel (NiCoT) and chromate (CHR). N. crassa has ... of the total membrane transport systems, which is higher than E. coli (8.8%), S. cerevisiae (13.7%), S. pombe (17.2%), A. ... P-ATPase, VIC, CaCA, Mid1), 7 for sodium (P-ATPase, CPA1, CPA2), 4 for potassium (Trk, VIC, KUP), and 4 for magnesium (MIT). ... Neurospora crassa has been the model filamentous fungus for the study of many fundamental cellular mechanisms of transport and ...
Figure 4 shows the effect of Arsenic on the activities membrane bound ATPase enzymes (Na+K+ ATPase, Ca2+ATPase and Mg2+ ATPase ... In this context, we evaluated the mode of action of chronic oral exposure of sodium arsenite as the source of arsenic at 5 mg/ ... Beutler E (1983) Active transport of glutathione disulfide from erythrocytes. In: Larson A, Orrenius S, Holmgren A, Mannerwik ... The membrane bound enzymes such as Na+/ K+-ATPase, Ca2+-ATPase and Mg2+-ATPase activity were assayed by estimating the amount ...