Argininosuccinate Lyase: An enzyme of the urea cycle which splits argininosuccinate to fumarate plus arginine. Its absence leads to the metabolic disease ARGININOSUCCINIC ACIDURIA in man. EC 4.3.2.1.Argininosuccinate Synthase: An enzyme of the urea cycle that catalyzes the formation of argininosuccinic acid from citrulline and aspartic acid in the presence of ATP. Absence or deficiency of this enzyme causes the metabolic disease CITRULLINEMIA in humans. EC 6.3.4.5.Argininosuccinic Aciduria: Rare autosomal recessive disorder of the urea cycle which leads to the accumulation of argininosuccinic acid in body fluids and severe HYPERAMMONEMIA. Clinical features of the neonatal onset of the disorder include poor feeding, vomiting, lethargy, seizures, tachypnea, coma, and death. Later onset results in milder set of clinical features including vomiting, failure to thrive, irritability, behavioral problems, or psychomotor retardation. Mutations in the ARGININOSUCCINATE LYASE gene cause the disorder.Argininosuccinic Acid: This amino acid is formed during the urea cycle from citrulline, aspartate and ATP. This reaction is catalyzed by argininosuccinic acid synthetase.Lyases: A class of enzymes that catalyze the cleavage of C-C, C-O, and C-N, and other bonds by other means than by hydrolysis or oxidation. (Enzyme Nomenclature, 1992) EC 4.CitrullineDucksArginase: A ureahydrolase that catalyzes the hydrolysis of arginine or canavanine to yield L-ornithine (ORNITHINE) and urea. Deficiency of this enzyme causes HYPERARGININEMIA. EC 3.5.3.1.Crystallins: A heterogeneous family of water-soluble structural proteins found in cells of the vertebrate lens. The presence of these proteins accounts for the transparency of the lens. The family is composed of four major groups, alpha, beta, gamma, and delta, and several minor groups, which are classed on the basis of size, charge, immunological properties, and vertebrate source. Alpha, beta, and delta crystallins occur in avian and reptilian lenses, while alpha, beta, and gamma crystallins occur in all other lenses.Arginine: An essential amino acid that is physiologically active in the L-form.Amino Acid Metabolism, Inborn Errors: Disorders affecting amino acid metabolism. The majority of these disorders are inherited and present in the neonatal period with metabolic disturbances (e.g., ACIDOSIS) and neurologic manifestations. They are present at birth, although they may not become symptomatic until later in life.Sodium Benzoate: The sodium salt of BENZOIC ACID. It is used as an antifungal preservative in pharmaceutical preparations and foods. It may also be used as a test for liver function.Citrullinemia: A group of diseases related to a deficiency of the enzyme ARGININOSUCCINATE SYNTHASE which causes an elevation of serum levels of CITRULLINE. In neonates, clinical manifestations include lethargy, hypotonia, and SEIZURES. Milder forms also occur. Childhood and adult forms may present with recurrent episodes of intermittent weakness, lethargy, ATAXIA, behavioral changes, and DYSARTHRIA. (From Menkes, Textbook of Child Neurology, 5th ed, p49)Ornithine Carbamoyltransferase: A urea cycle enzyme that catalyzes the formation of orthophosphate and L-citrulline (CITRULLINE) from CARBAMOYL PHOSPHATE and L-ornithine (ORNITHINE). Deficiency of this enzyme may be transmitted as an X-linked trait. EC 2.1.3.3.Urea: A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.Ornithine: An amino acid produced in the urea cycle by the splitting off of urea from arginine.Ligases: A class of enzymes that catalyze the formation of a bond between two substrate molecules, coupled with the hydrolysis of a pyrophosphate bond in ATP or a similar energy donor. (Dorland, 28th ed) EC 6.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.ATP Citrate (pro-S)-Lyase: An enzyme that, in the presence of ATP and COENZYME A, catalyzes the cleavage of citrate to yield acetyl CoA, oxaloacetate, ADP, and ORTHOPHOSPHATE. This reaction represents an important step in fatty acid biosynthesis. This enzyme was formerly listed as EC 4.1.3.8.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Comamonadaceae: A family of gram-negative aerobic bacteria in the class BETA PROTEOBACTERIA, encompassing the acidovorans rRNA complex. Some species are pathogenic for PLANTS.Comamonas: A genus of gram-negative, straight or slightly curved rods which are motile by polar flagella and which accumulate poly-beta-hydroxybutyrate within the cells.Nitric Oxide: A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.Multiple Organ Failure: A progressive condition usually characterized by combined failure of several organs such as the lungs, liver, kidney, along with some clotting mechanisms, usually postinjury or postoperative.Nitric Oxide Synthase: An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.Urea Cycle Disorders, Inborn: Rare congenital metabolism disorders of the urea cycle. The disorders are due to mutations that result in complete (neonatal onset) or partial (childhood or adult onset) inactivity of an enzyme, involved in the urea cycle. Neonatal onset results in clinical features that include irritability, vomiting, lethargy, seizures, NEONATAL HYPOTONIA; RESPIRATORY ALKALOSIS; HYPERAMMONEMIA; coma, and death. Survivors of the neonatal onset and childhood/adult onset disorders share common risks for ENCEPHALOPATHIES, METABOLIC, INBORN; and RESPIRATORY ALKALOSIS due to HYPERAMMONEMIA.Hyperammonemia: Elevated level of AMMONIA in the blood. It is a sign of defective CATABOLISM of AMINO ACIDS or ammonia to UREA.Ornithine Carbamoyltransferase Deficiency Disease: An inherited urea cycle disorder associated with deficiency of the enzyme ORNITHINE CARBAMOYLTRANSFERASE, transmitted as an X-linked trait and featuring elevations of amino acids and ammonia in the serum. Clinical features, which are more prominent in males, include seizures, behavioral alterations, episodic vomiting, lethargy, and coma. (Menkes, Textbook of Child Neurology, 5th ed, pp49-50)Metabolism, Inborn Errors: Errors in metabolic processes resulting from inborn genetic mutations that are inherited or acquired in utero.Hyperargininemia: A rare autosomal recessive disorder of the urea cycle. It is caused by a deficiency of the hepatic enzyme ARGINASE. Arginine is elevated in the blood and cerebrospinal fluid, and periodic HYPERAMMONEMIA may occur. Disease onset is usually in infancy or early childhood. Clinical manifestations include seizures, microcephaly, progressive mental impairment, hypotonia, ataxia, spastic diplegia, and quadriparesis. (From Hum Genet 1993 Mar;91(1):1-5; Menkes, Textbook of Child Neurology, 5th ed, p51)Diabetes Mellitus, Experimental: Diabetes mellitus induced experimentally by administration of various diabetogenic agents or by PANCREATECTOMY.Diabetic Nephropathies: KIDNEY injuries associated with diabetes mellitus and affecting KIDNEY GLOMERULUS; ARTERIOLES; KIDNEY TUBULES; and the interstitium. Clinical signs include persistent PROTEINURIA, from microalbuminuria progressing to ALBUMINURIA of greater than 300 mg/24 h, leading to reduced GLOMERULAR FILTRATION RATE and END-STAGE RENAL DISEASE.Streptozocin: An antibiotic that is produced by Stretomyces achromogenes. It is used as an antineoplastic agent and to induce diabetes in experimental animals.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Gene Library: A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Transcriptome: The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells.Magnesium Oxide: Magnesium oxide (MgO). An inorganic compound that occurs in nature as the mineral periclase. In aqueous media combines quickly with water to form magnesium hydroxide. It is used as an antacid and mild laxative and has many nonmedicinal uses.Speech-Language Pathology: The study of speech or language disorders and their diagnosis and correction.Ammonia: A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.Carcinoma, Hepatocellular: A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.Liver Neoplasms: Tumors or cancer of the LIVER.MedlinePlus: NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.Health Records, Personal: Longitudinal patient-maintained records of individual health history and tools that allow individual control of access.Nitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Dinoflagellida: Flagellate EUKARYOTES, found mainly in the oceans. They are characterized by the presence of transverse and longitudinal flagella which propel the organisms in a rotating manner through the water. Dinoflagellida were formerly members of the class Phytomastigophorea under the old five kingdom paradigm.Harmful Algal Bloom: An algal bloom where the algae produce powerful toxins that can kill fish, birds, and mammals, and ultimately cause illness in humans. The harmful bloom can also cause oxygen depletion in the water due to the death and decomposition of non-toxic algae species.Marine Toxins: Toxic or poisonous substances elaborated by marine flora or fauna. They include also specific, characterized poisons or toxins for which there is no more specific heading, like those from poisonous FISHES.Oxocins: Compounds based on an 8-membered heterocyclic ring including an oxygen. They can be considered medium ring ethers.Nitrogen Compounds: Inorganic compounds that contain nitrogen as an integral part of the molecule.Dianisidine: Highly toxic compound which can cause skin irritation and sensitization. It is used in manufacture of azo dyes.

Glucocorticoids mediate the enhanced expression of intestinal type II arginase and argininosuccinate lyase in postweaning pigs. (1/123)

Arginine metabolism is enhanced in the small intestine of weanling pigs, but the molecular mechanism(s) involved is not known. The objectives of this study were to determine the following: 1) whether glucocorticoids play a role in induction of intestinal arginine metabolic enzymes during weaning; 2) whether the induction of enzyme activities was due to increases in corresponding mRNA levels; and 3) the identity of the arginase isoform(s) expressed in the small intestine. Jejunum was obtained from 29-d-old weaned pigs that were or were not treated with 17-beta-hydroxy-11beta-(4-dimethylaminophenyl)17alpha-(prop- 1-ynyl)es tra-4,9-dien-3-one (RU486, an antagonist of glucocorticoid receptors), or from age-matched suckling pigs. Activities and mRNA levels for type I and type II arginases, argininosuccinate synthase (ASS) and argininosuccinate lyase (ASL) were determined. Activities of arginase, ASL and ASS increased by 635, 56 and 106%, respectively, in weanling pigs, compared with suckling pigs. RU486 treatment attenuated the increase in arginase activity by 74% and completely prevented the ASL induction in weanling pigs, but had no effect on ASS activity. Pig intestine expresses both type I and type II arginases. On the basis of immunoblot analyses, there was no significant difference in levels of intestinal type I arginase among these three groups of pigs, indicating that changes in arginase activity were due only to type II arginase. The mRNA levels for type II arginase and ASL increased by 135 and 198%, respectively, in weanling pigs compared with suckling pigs, and this induction was completely prevented by RU486. In contrast, ASS mRNA levels did not differ between suckling and weanling pigs. These results suggest that intestinal type II arginase, ASS and ASL are regulated differentially at transcriptional and post-translational levels and that glucocorticoids play a major role in the induction of type II arginase and ASL mRNAs in the small intestine of weanling pigs.  (+info)

Competitive inactivation of a double-strand DNA break site involves parallel suppression of meiosis-induced changes in chromatin configuration. (2/123)

In Saccharomyces cerevisiae, DNA double-strand breaks (DSBs) initiate meiotic recombination at open sites in chromatin, which display a meiosis-specific increase in micrococcal nuclease (MNase) sensitivity. The arg4 promoter contains such a DSB site. When arg4 sequences are placed in a pBR322-derived insert at HIS4 (his4 :: arg4 ), the presence of strong DSB sites in pBR322 sequences leads to an almost complete loss of breaks from the insert-borne arg4 promoter region. Most of the MNase-sensitive sites occurred at similar positions in insert-borne and in normal ARG4 sequences, indicating that hotspot inactivation is not a consequence of changes in nucleosome positioning. However, a meiosis-specific increase in MNase hypersensitivity was no longer detected at the inactive insert-borne arg4 DSB site. Elimination of pBR322 sequences restored DSBs to the insert-borne arg4 promoter region and also restored the meiotic induction of MNase hypersensitivity. Thus, the meiotic induction of MNase hypersensitivity at the DSB sites is suppressed and activated in parallel to DSBs themselves, without changes in the underlying DNA sequence or nucleosome positioning. We suggest that meiosis-specific changes in chromatin at a DSB site are a signal reflecting a pivotal step in DSB formation.  (+info)

Expression of Escherichia coli K-12 arginine genes in Pseudomonas fluorescens. (3/123)

Escherichia coli argE and argH gene products were detected in Pseudomonas fluorescens argH122 carrying the E. coli F110 plasmid.  (+info)

Use of a recombination reporter insert to define meiotic recombination domains on chromosome III of Saccharomyces cerevisiae. (4/123)

In Saccharomyces cerevisiae, meiotic recombination is initiated by DNA double-strand breaks (DSBs). DSBs usually occur in intergenic regions that display nuclease hypersensitivity in digests of chromatin. DSBs are distributed nonuniformly across chromosomes; on chromosome III, DSBs are concentrated in two "hot" regions, one in each chromosome arm. DSBs occur rarely in regions within about 40 kb of each telomere and in an 80-kb region in the center of the chromosome, just to the right of the centromere. We used recombination reporter inserts containing arg4 mutant alleles to show that the "cold" properties of the central DSB-deficient region are imposed on DNA inserted in the region. Cold region inserts display DSB and recombination frequencies that are substantially less than those seen with similar inserts in flanking hot regions. This occurs without apparent change in chromatin structure, as the same pattern and level of DNase I hypersensitivity is seen in chromatin of hot and cold region inserts. These data are consistent with the suggestion that features of higher-order chromosome structure or chromosome dynamics act in a target sequence-independent manner to control where recombination events initiate during meiosis.  (+info)

(CA/GT)(n) microsatellites affect homologous recombination during yeast meiosis. (5/123)

One of the most common microsatellites in eukaryotes consists of tandem arrays of the dinucleotide GT. Although the study of the instability of such repetitive DNA has been extremely fruitful over the last decade, no biological function has been demonstrated for these sequences. We investigated the genetic behavior of a region of the yeast Saccharomyces cerevisiae genome containing a 39-CA/GT dinucleotide repeat sequence. When the microsatellite sequence was present at the ARG4 locus on homologous chromosomes, diploid cells undergoing meiosis generated an excess of tetrads containing a conversion of the region restricted to the region of the microsatellite close to the recombination-initiation double-strand break. Moreover, whereas the repetitive sequence had no effect on the frequency of single crossover, its presence strongly stimulated the formation of multiple crossovers. The combined data strongly suggest that numerous recombination events are restricted to the initiation side of the microsatellite as though progression of the strand exchange initiated at the ARG4 promoter locus was impaired by the repetitive sequence. This observation corroborates in vitro experiments that demonstrated that RecA-promoted strand exchange is inhibited by CA/GT dinucleotide tracts. Surprisingly, meiotic instability of the microsatellite was very high (>0.1 alterations per tetrad) in all the spores with parental and recombinant chromosomes.  (+info)

Guanidine hydrochloride induced reversible dissociation and denaturation of duck delta2-crystallin. (6/123)

The tetrameric delta2-crystallin from duck lens exhibits a reversible dissociation-denaturation process in solutions containing guanidine hydrochloride (GdnHCl). Sigmoidal or biphasic curves for the dissociation/denaturation processes, obtained using different methods of structural analysis, as a function of GdnHCl concentration were not coincidental with each other. delta2-crystallin in 0.91 M GdnHCl existed primarily as a monomer, which had no endogenous argininosuccinate lyase activity. After dilution of the GdnHCl-treated protein, the monomers reassociated into tetramers with concomitant recovery of enzyme activity. The sigmoidal recovery of enzyme activity demonstrates a cooperative hysteretic reactivation process. When the concentration of GdnHCl was higher than 1.2 M, various partially unfolded soluble forms of delta2-crystallin were produced from the dissociated monomers as shown by size-exclusion chromatography. The formation of a partially unfolded intermediate during the dissociation-denaturation process is proposed.  (+info)

Regulation of diaphragmatic nitric oxide synthase expression during hypobaric hypoxia. (7/123)

Nitric oxide (NO) is normally synthesized inside skeletal muscle fibers by both endothelial (eNOS) and neuronal (nNOS) nitric oxide synthases. In this study, we evaluated the influence of hypobaric hypoxia on the expression of NOS isoforms, argininosuccinate synthetase (AS), argininosuccinate lyase (AL), and manganese superoxide dismutase (Mn SOD) in the ventilatory muscles. Rats were exposed to hypobaric hypoxia ( approximately 95 mmHg) from birth for 60 days or 9-11 mo. Age-matched control groups of rats also were examined. Sixty days of hypoxia elicited approximately two- and ninefold increases in diaphragmatic eNOS and nNOS protein expression (evaluated by immunoblotting), respectively, and about a 50% rise in diaphragmatic NOS activity. In contrast, NOS activity and the expression of these proteins declined significantly in response to 9 mo of hypoxia. Hypoxia elicited no significant alterations in AS, AL and Mn SOD protein expression. Moreover, the inducible NOS (iNOS) was not detected in normoxic and hypoxic diaphragmatic samples. We conclude that diaphragmatic NOS expression and activity undergo significant adaptations to hypobaric hypoxia and that iNOS does not participate in this response.  (+info)

Accumulation of arginine precursors in Escherichia coli: effects on growth, enzyme repression, and application to the forward selection of arginine auxotrophs. (8/123)

The accumulation or ornithine, citrulline, and possibly acetylornithine by Escherichia coli K-12 arginineless mutants provided with acetylarginine as source of arginine causes severe growth inhibition. This occurs under conditions where comparable derivatives of E. coli W (Bollon and Vogel, 1973) show little or no growth inhibition. The same conditions, which have been reported to cause noncorrelative synthesis of acetylornithinase and argininosuccinase in E. coli W (Bollon and Vogel, 1973), do not alter the correlative pattern of enzyme synthesis observed in E. coli K-12. Moreover, previously reported effects of ornithine and citrulline on repression of the arginine regulon in E. coli W are not observed in the K-12 strains examined. The bearing of these observations on possible differences between the mechanism of enzyme repression operating in the two types of strains cannot yet be fully evaluated; it is, however, clear that considerable care should be exercised before extrapolating the results obtained with one type of strain to the other one. The particularly strong inhibition of acetylarginine utilization exerted by ornithine in E. coli K-12 allows the forward selection of several classes of arginine auxotrophs from strains deficient in carbamoylphosphate biosynthesis and thus capable of ornithine accumulation. Possible applications of this technique to the genetic analysis of the bipolar argECBH operon are discussed.  (+info)

TY - JOUR. T1 - Requirement of argininosuccinate lyase for systemic nitric oxide production. AU - Erez, Ayelet. AU - Nagamani, Sandesh C.S.. AU - Shchelochkov, Oleg A.. AU - Premkumar, Muralidhar H.. AU - Campeau, Philippe M.. AU - Chen, Yuqing. AU - Garg, Harsha K.. AU - Li, Li. AU - Mian, Asad. AU - Bertin, Terry K.. AU - Black, Jennifer O.. AU - Zeng, Heng. AU - Tang, Yaoping. AU - Reddy, Anilkumar K.. AU - Summar, Marshall. AU - OBrien, William E.. AU - Harrison, David G.. AU - Mitch, William E.. AU - Marini, Juan C.. AU - Aschner, Judy L.. AU - Bryan, Nathan S.. AU - Lee, Brendan. PY - 2011/12/1. Y1 - 2011/12/1. N2 - Nitric oxide (NO) is crucial in diverse physiological and pathological processes. We show that a hypomorphic mouse model of argininosuccinate lyase (encoded by Asl) deficiency has a distinct phenotype of multiorgan dysfunction and NO deficiency. Loss of Asl in both humans and mice leads to reduced NO synthesis, owing to both decreased endogenous arginine synthesis and an ...
TY - JOUR. T1 - Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells. AU - Hung, Yu Hsuan. AU - Huang, Hau Lun. AU - Chen, Wei Ching. AU - Yen, Meng Chi. AU - Cho, Chien Yu. AU - Weng, Tzu Yang. AU - Wang, Chih Yang. AU - Chen, Yi Ling. AU - Chen, Li Tzong. AU - Lai, Ming Derg. PY - 2017/2. Y1 - 2017/2. N2 - Arginine is a critical amino acid in specific cancer types including hepatocellular carcinoma (HCC) and melanoma. Novel molecular mechanisms and therapeutic targets in arginine metabolism-mediated cancer formation await further identification. Our laboratory has previously demonstrated that arginine metabolic enzyme argininosuccinate lyase (ASL) promoted HCC formation in part via maintenance of cyclin A2 protein expression and arginine production for channeling to nitric oxide synthase. In this study, we investigated the mechanism by which ASL regulates cyclin A2 expression. We found that ASL interacted with cyclin A2 in HCC cells and the ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
ID ARLY_AGRVS Reviewed; 467 AA. AC B9JTJ2; DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot. DT 24-MAR-2009, sequence version 1. DT 25-OCT-2017, entry version 54. DE RecName: Full=Argininosuccinate lyase {ECO:0000255,HAMAP-Rule:MF_00006}; DE Short=ASAL {ECO:0000255,HAMAP-Rule:MF_00006}; DE EC=4.3.2.1 {ECO:0000255,HAMAP-Rule:MF_00006}; DE AltName: Full=Arginosuccinase {ECO:0000255,HAMAP-Rule:MF_00006}; GN Name=argH {ECO:0000255,HAMAP-Rule:MF_00006}; GN OrderedLocusNames=Avi_4017; OS Agrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis OS (strain S4)). OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium. OX NCBI_TaxID=311402; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=S4 / ATCC BAA-846; RX PubMed=19251847; DOI=10.1128/JB.01779-08; RA Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K., RA Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I., RA Otten L., Suen G., ...
You ever have one of those months? I feel like I have been at the vet every other day for the last month. New pup visit with poop samples and
My responds - of course, I was mad and a bit confused. To clear my thought, I started to dig lot of journals regarding the chemotherapy regiment for GTD and the GTD itself. Found out that 5% of patients that undergo the MTX-FA will start to develop resistant towards the drugs. Argh… (again I considered myself as lucky-one to be in the statistical data ...
Argh! Im a week overdue with, what is predicted to be a BIG Bubba (no.2) & going for a sweep tomorrow. I just wondered, in your collective experienc
Although accumulating evidence highlights the importance of p53-mediated metabolism in tumor suppression (2, 47), the mechanisms by which p53 drives dynamic nutrient status in harmony with canonical p53 functions remain poorly understood. Here, we show that p53 activates the penultimate step of de novo arginine synthesis pathway through the direct induction of the rate-limiting enzyme ASS1. Furthermore, we demonstrate that ASS1 deficiency induced anomalous Akt phosphorylation, resulting in rendering cells more susceptible to genotoxic stress.. Although we have demonstrated that p53 drives the de novo arginine synthesis pathway via ASS1 induction under genotoxic conditions, argininosuccinate lyase (ASL), which directly produces arginine from argininosuccinate, was not induced by p53 in HCT116 cells. These results suggest that ASS1 is the sole node connecting p53 to the de novo arginine synthesis pathway. Because ASS1 is a rate-limiting enzyme of the de novo arginine synthesis pathway, ASS1 ...
TY - JOUR. T1 - Targeting argininosuccinate synthetase negative melanomas using combination of arginine degrading enzyme and cisplatin. AU - Savaraj, Niramol. AU - Wu, Chunjing. AU - Li, Ying Ying. AU - Wangpaichitr, Medhi. AU - You, Min. AU - Bomalaski, John. AU - He, Wei. AU - Kuo, Macus Tien. AU - Feun, Lynn G. PY - 2015. Y1 - 2015. N2 - Loss of argininosuccinate synthetase (ASS) expression in melanoma makes these tumor cells vulnerable to arginine deprivation. Pegylated arginine deiminase (ADIPEG20) which degrades arginine to citrulline and ammonia has been used clinically and partial responses and stable disease have been noted with minimal toxicity. In order to improve the therapeutic efficacy of ADI-PEG20, we have combined ADI-PEG20 with a DNA damaging agent, cisplatin. We have shown that the combination of the two drugs together significantly improved the therapeutic efficacy when compared to ADI-PEG20 alone or cisplatin alone in 4 melanoma cell lines, regardless of their BRAF mutation. ...
ID ARLY_METST Reviewed; 465 AA. AC Q2NGN7; DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot. DT 07-FEB-2006, sequence version 1. DT 25-OCT-2017, entry version 79. DE RecName: Full=Argininosuccinate lyase {ECO:0000255,HAMAP-Rule:MF_00006}; DE Short=ASAL {ECO:0000255,HAMAP-Rule:MF_00006}; DE EC=4.3.2.1 {ECO:0000255,HAMAP-Rule:MF_00006}; DE AltName: Full=Arginosuccinase {ECO:0000255,HAMAP-Rule:MF_00006}; GN Name=argH {ECO:0000255,HAMAP-Rule:MF_00006}; GN OrderedLocusNames=Msp_0618; OS Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / OS MCB-3). OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; OC Methanobacteriaceae; Methanosphaera. OX NCBI_TaxID=339860; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3; RX PubMed=16385054; DOI=10.1128/JB.188.2.642-658.2006; RA Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., RA Hedderich R., Gottschalk G., Thauer R.K.; RT "The genome sequence of ...
Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):E5006-15. doi: 10.1073/pnas.1321305110. Epub 2013 Dec 2. Research Support, N.I.H., Extramural
For example, the fact that methylation-dependent silencing of argininosuccinate synthetase (ASS1) [40], a rate-limiting enzyme involved in the biosynthesis of arginine, has been implicated in therapeutic resistance in several cancer types including renal cell carcinoma, hepatocellular carcinoma, malignant melanoma, glioblastoma multiforme, and platinum-resistant epithelial. ovarian cancer suggests a role for demethylating agents in these ASS1 drug-resistant find more cancers [41]. Nevertheless, despite their current nonspecific promiscuity, epigenetic agents may act on most or all tumor types, since aberrant methylation and deacetylation patterns are a hallmark of cancer cells. In particular, several of the anticancer agents described in this review activate and upregulate p53, which itself affects multiple targets [19]. Following genotoxic stress. in response to traditional therapeutic strategies such as cisplatin, doxorubicin, 5-fluorouracil, fludarabine, mitoxantrone, etoposide, or X-ray ...
My scalp is itching and bumpy! I usually use Philip Kingsley itchy flaky shampoo and that seems to sort it out. But its so expensive. [sad] - does
Argh! First, the morning starts off when Im on my new diet/exercise program (oh, help…) and then you make me cry with the doggie video….. And now I have a combination of the slinky tune and the log tune from ren and stimpy stuck in my head….. @ Samantha, that popped in my head too! And it wont go away!!. ...
And, yes, the ultra sound showed that I had an enlarged thyroid with nodules. Could this possibly be the cause of my chronic cough that has progressively gotten worse over the past 7 or 8 years? At first it looked like the cough was solved. But gradually over the month or so since the surgery, Ive started coughing again. Not as much, but its still there. ARGH! Hope I didnt go through this for nothing ...
And, yes, the ultra sound showed that I had an enlarged thyroid with nodules. Could this possibly be the cause of my chronic cough that has progressively gotten worse over the past 7 or 8 years? At first it looked like the cough was solved. But gradually over the month or so since the surgery, Ive started coughing again. Not as much, but its still there. ARGH! Hope I didnt go through this for nothing ...
Broad said: Like I said, my feelings are complicated on the matter, so ... Im interested, however, in Her Highness thoughts on… ...[go].. Caterina said: ARGH!!! Not to deny you your goddess-given right of reflections and wishing what might-have-beens, but this guy was straight up… ...[go].. Wholovesya? said: By the by, guess who was most nasty about the charitable giving? The frigging church. My church and my moms… ...[go].. Wholovesya? said: By the by, Im not the only one I know. I have friends who work at soup kitchens because theyre… ...[go].. Wholovesya? said: As you know, I was a voyeur to the beginning of this, and I was loving your comment! I have… ...[go].. ...
Broad said: Like I said, my feelings are complicated on the matter, so ... Im interested, however, in Her Highness thoughts on… ...[go].. Caterina said: ARGH!!! Not to deny you your goddess-given right of reflections and wishing what might-have-beens, but this guy was straight up… ...[go].. Wholovesya? said: By the by, guess who was most nasty about the charitable giving? The frigging church. My church and my moms… ...[go].. Wholovesya? said: By the by, Im not the only one I know. I have friends who work at soup kitchens because theyre… ...[go].. Wholovesya? said: As you know, I was a voyeur to the beginning of this, and I was loving your comment! I have… ...[go].. ...
Yes you did!". "No I didnt!". "It was obviously you, who else would be so clumsy and careless?!". (sound of a door slamming). "See Im right! Childish fool! Cant get any sense into your thick head! Argh!!" Taeyeon yelled. He had been arguing with his younger brother Taejin the whole night over a trivial matter that grew out of proportion. Feeling very frustrated, he decided to get out of the house to cool himself down.. Grabbing a few items from his room, Taeyeon told his mother he would be staying over at a friends house tonight before he stormed out of the door.. ~*~*~*~. Ping. A tiny stone bounced off the window. Taeyeon waited to see if there was any movement. After two minutes, there was none besides the rustling of the leaves in the light breeze. He picked up another small stone and threw it against the window.. Ping. Nothing. Anxious, Taeyeon pulled out his mobile phone from his back pocket to type a message. He sat cross legged on the ground, with his back against the door of the ...
Treatment may require the coordinated efforts of a team of specialists. Pediatricians, neurologists, geneticists, dieticians, and physicians who are familiar with metabolic disorders may need to work together to ensure a comprehensive approach to treatment. Occupational, speech language, and physical therapists may be needed to treat children with developmental disabilities. Genetic counseling is recommended for affected individuals and their families.. The treatment of argininosuccinic aciduria is aimed at preventing excessive ammonia from being formed or from removing excessive ammonia during a hyperammonemic episode. Long-term therapy combines dietary restrictions and the stimulation of alternative methods of converting and excreting nitrogen from the body (alternative pathways therapy).. Dietary restrictions in individuals with argininosuccinic aciduria are aimed at limiting the amount of protein intake to avoid the development of excess ammonia. However, enough protein must be taken in by ...
Argininosuccinate lyase deficiency (ASLD; also known as argininosuccinic aciduria) is the second most common urea cycle disorder (UCD) and accounts for 15-20% of all disorders of ureagenesis. Individuals with ASLD can have unique clinical and physiologic characteristics as compared to other UCDs. Previous work from the members of the UCDC have shown that in spite of having fewer episodes of hyperammonemia as compared to those with proximal blockade of the urea cycle, individuals with ASLD can develop intellectual and learning disabilities. Neurocognitive deficits have been observed even in individuals without any documented hyperammonemia. Furthermore, hepatic abnormalities including hepatomegaly, hepatic injury, fibrosis and even frank cirrhosis, and vascular issues like hypertension are well known in the disorder. Previous work from the members of the UCDC has demonstrated a tissue- and molecular-specific role for ASL in the generation of NO. ASL is not only required for the synthesis of ...
... On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms or a full patient history. A similarity measure between symptoms and diseases is provided.
Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype. This is a comprehensive report of all 87 mutations found to date in the ASS1 gene on chromosome 9q34.1. A large proportion of the mutations (n=27) are described here for the first time. Mutations are distributed throughout exons 3 to 15, most of them being identified in exons 5, 12, 13, and 14. The mutation G390R in exon 15 is the single most common mutation in patients with the classical phenotype. Certain mutations clearly link to specific clinical courses but the clinical phenotype cannot be anticipated in all patients. This update presents a survey of the correlation between mutations in the ASS1 gene and the respective clinical courses as ...
In the linear pathway (Figure 1A), GLU is converted to acetylglutamate (Ac-GLU) by N-acetylglutamate synthase (NAGS, encoded by argA) which is inhibited by ARG through negative feedback regulation [36],[39]. Sequential catalytic reactions catalyzed by the next three enzymes, N-acetylglutamate kinase (NAGK, encoded by argB), N-acetylglutamate semialdehyde dehydrogenase (encoded by argC) and N-acetylornithine transaminase (encoded by argD), which are common in the three pathways (Figure 1), yield N-acetylornithine (Ac-ORN) [34]. The next step, which distinguishes the linear pathway from the other two pathways, is deacetylation of Ac-ORN by AOase to yield ORN [40],[41]. The next and final steps are carried out by ornithine carbamoyltransferase (OTC or OTCase, encoded by argF), argininosuccinate synthase (encoded by argG) and argininosuccinate lyase (encoded by argH), which finally yield ARG [35]. This pathway has been found in a few species such as Myxococcus xanthus [41] and E. coli [36].. In many ...
Pancreatic cancer is a leading cause of cancer-related deaths in the world with a 5-year survival rate of less than 6%. Currently, there is no successful therapeutic strategy for advanced pancreatic cancer, and new effective strategies are urgently needed. Recently, an arginine deprivation agent, arginine deiminase, was found to inhibit the growth of some tumor cells (i.e., hepatocellular carcinoma, melanoma, and lung cancer) deficient in argininosuccinate synthetase (ASS), an enzyme used to synthesize arginine. The purpose of this study was to evaluate the therapeutic efficacy of arginine deiminase in combination with gemcitabine, the first line chemotherapeutic drug for patients with pancreatic cancer, and to identify the mechanisms associated with its anticancer effects. In this study, we first analyzed the expression levels of ASS in pancreatic cancer cell lines and tumor tissues using immunohistochemistry and RT-PCR. We further tested the effects of the combination regimen of arginine deiminase
Kleijer WJ et al. (2002) Clinical, enzymatic, and molecular genetic characterization of a biochemical variant type of argininosuccinic aciduria: prenatal and postnatal diagnosis in five unrelated families.. [^] ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Sreekumar A, Poisson LM, Rajendiran TM, Khan AP, Cao Q, Yu J, Laxman B, Mehra R, Lonigro RJ, Li Y, Nyati MK, Ahsan A, Kalyana-Sundaram S, Han B, Cao X, Byun J, Omenn GS, Ghosh D, Pennathur S, Alexander DC, Berger A, Shuster JR, Wei JT, Varambally S, Beecher C, Chinnaiyan AM: Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression. Nature. 2009 Feb 12;457(7231):910-4. Pubmed: 19212411 ...
1KH2: Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action.
Complete information for ASS1P14 gene (Pseudogene), Argininosuccinate Synthetase 1 Pseudogene 14, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ASS1P12 gene (Pseudogene), Argininosuccinate Synthetase 1 Pseudogene 12, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
SWISS-MODEL Template Library (SMTL) entry for 1kh3.1. Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with inhibitor
David is a young hobby astronomer, who one day accidentally shots down an alien spacecraft by his self-made model rocket. In just that moment the UFO crashes in front of him his own rocket knocks him out. He awakes on a foreign farm and has to find out...
Then a few days later I want to do a show ip bgp hmm output not as expected. Right this time I twig it has to be one of those pesky VRFs getting in my way. So lets try doing show ip vrf abc wooh no bgp command. Okay lets try figure this out show ip ? and look through the options hmm nothing there so how about a show ip bgp ? hmm nothing there either. Argh so I am stumped how the heck do you do a show ip bgp when you have VRFs around? Time to prepare colleague for another stupid question by offering to make him a drink. Once drink has been made then I utilize my chance for another stupid question. "Umm so you know these VRFs how do you do a show ip bgp turns out the answer is to do a show ip bgp vpnv4 vrf abc. So I have enough trouble with VRFs and then they go add the vpnv4 command before the vrf command. Guess I will get to that bit when I sort out multi-protocol BGP. Anyhow you will be pleased to know that I am getting on a lot better with VRFs now. However as with anything on this blog ...
I feel like one huge swollen tonsil. Like my eustachian tubes (etruscan tubes? I cant spell yoostayshun) are on fire. The back of my throat has rabid bats roosting in it, dropping guano....burning guano, down my esophagus. And to make it even more annoying, this has been slowly slowwwwwwly coming on for two damn weeks.…
Scores of would-be swashbucklers found themselves in need of rescuing Saturday night, when their ship -- the Pirates Ransom -- ran aground in west Florida.
Hospital Escape : Argh, I dont like hospitals! Out, please! If only I wasnt drugged, this might be easier. And where are the nurses? Free Online Puzzle Games from AddictingGames
Have you ever had a day like this? Overslept. Didnt accomplish what I wanted. Things in the house broke. My phone kept sticking and not letting me type. ARGH! Drivers on the rainy roads drove crazy (that may have been because of my grumpy mood). I basically flailed on the edge of losing it all day long. Continue Reading…. ...
So glad she does not have the flu. I know the anxiety you are having. It sounds like A has a lot of extra health issues that make having the flu a lot of extra work and even more scary. Same with M. I am constantly questioning what I am doing in order to help her stay healthy. I cant keep her in a bubble but when it is all over her school I can keep her home. Does that do any good in the long run since these outbreaks could go on and then there are different viruses anyway that can send her into CVS and then to the hospital. Sigh. Argh. I go around and around about it. ...
Argh! I hate when I hear something cool on the radio from the other room and bring up the playlist to see who it is and its Steve Reich ...
And by that time, Im past him and spend the rest of my ride home going back and forth in this way. Im sick of it. Maybe one day I should just come stand next to you and do the same thing, or when I forget my wallet, ask you for some change. Argh.. 3. Apple basket Pirates. Back off. Seriously. If you understand that analogy, great. If not, and you want to, just ask. 4. Two dimensional thinkers. Im used to being around people who, when faced with a problem or something they dont understand, try and figure it out on their own before asking everyone around them to do it for them. Those people are three dimensional thinkers-I would even go so far as to say they are multi-dimensional thinkers. Two dimensional thinkers make zero effort to figure something out on their own and hand it off to other people. "Oh. This doesnt work. Rather than making an effort to be intelligent and solve the problem myself, Ill just call ten other people and make them do it for me because Im intellectually lazy." Im ...
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From the NYT: The Census Bureau, the authoritative source of health insurance data for more than three decades, is changing its annual survey so thoroughly that
0/3: When I first started smoking cigars I smoked a lot of Alec Bradley cigars and while there are definitely some good cigars in their lineup (I especially like the Prensado and the Family Blend) I found myself smoking less and less of them as time went on. Maybe my tastes have changed or maybe there are just too many good cigars out there that I just lost touch with Alec Bradley cigars.. But then I saw that Alec Bradley had put out their first Nicaraguan puro, aptly named the Nica Puro, and I wanted to try it to see how good it was.. It is a good looking cigar, not a lot of oils on the wrapper, nor veins, noticeably not symmetrical but pretty close. Mostly consistently packed.. 1/3: How is the world suppose to end? Does it include fire raining down on the sinful masses? Well, my shorts just received their own little micro-apocalypse after I fumbled with the cigar and knocked newly lit tobacco cherries off of the foot and they fell on my shorts like a shower of small asteroids. Argh!. The cigar ...
0/3: When I first started smoking cigars I smoked a lot of Alec Bradley cigars and while there are definitely some good cigars in their lineup (I especially like the Prensado and the Family Blend) I found myself smoking less and less of them as time went on. Maybe my tastes have changed or maybe there are just too many good cigars out there that I just lost touch with Alec Bradley cigars.. But then I saw that Alec Bradley had put out their first Nicaraguan puro, aptly named the Nica Puro, and I wanted to try it to see how good it was.. It is a good looking cigar, not a lot of oils on the wrapper, nor veins, noticeably not symmetrical but pretty close. Mostly consistently packed.. 1/3: How is the world suppose to end? Does it include fire raining down on the sinful masses? Well, my shorts just received their own little micro-apocalypse after I fumbled with the cigar and knocked newly lit tobacco cherries off of the foot and they fell on my shorts like a shower of small asteroids. Argh!. The cigar ...
Argh. All year (and for a couple of years before I got to grad school) Ive been angsting about whether I really want to get into academia. These posts seem to confirm my worst fears about ivory-tower irrelevancy and uncertain rewards.. Its why I talked myself into moving into a research area with potential applications - technically in the electrical engineering department. Im feeling very good about that, after reading this.. Of course thinking about big ideas is more fun than solving problems, but that doesnt make it more noble. The more I hear theorists talk about "pure science" and their own enormous importance, the more I want to be an engineer, a teacher, a popularizer… Anything but a "pure scientist". Ill bet I can do all of that and still think about the nature of reality just as successfully as they do.. ...
So now there are 21 proteogenic amino acids…right?. Actually, there are 22 of them discovered till now. The last one is named Pyrrolysine, but it makes proteins only in prokaryotes (methanogenic archaea and bacteria).. So is there any other amino acids that are not "PROTEOGENIC"?. The proteogenic pool of amino acid is only a small part of the whole amino acid pool. There are many times more amino acids that dont produce proteins. Usually, the amino acids that dont have any associated genetic code wont produce proteins (they cant take part in translation), and so are non-proteogenic.. This is quite logical. Any organic molecule having at least one of each amine and carboxyl group can be called an amino acid and we have only limited number of genetic codes.. Examples of non-proteogenic amino acids are - ornithine, citrulline etc. in hepatic metabolism. The argininosuccinic acid in the urea cycle, S-adenosylmethionine, azaserine in streptomycin, synthetically produced (at least commercially) ...
Many melanomas do not express argininosuccinate synthetase (ASS) which is a key enzyme in the process of arginine biosynthesis. As a result, these melanoma cells need exogenous arginine supply for survival and proliferation while normal cell do not. A new drug targeting this metabolic defect of melanoma has been developed by Polaris, Inc. (recombinant arginine deiminase conjugated with polyethylene glycol-20 or ADI-PEG20). This complex can effectively degrade blood arginine to citrulline. Phase I/II clinical trials with ADI-PEG20 have shown promising activity in patients with advanced melanoma. However, our laboratory studies have demonstrated that after treatment with ADI-PEG20, some melanoma cells are able to induce the production of ASS, while others have the ability to undergo prolonged autophagy as well as low requirement for arginine to survive. Thus, arginine deprivation results in growth inhibition without cell death. On the other hand, TRAIL has been used to induce apoptosis in certain ...
Principal Investigator:MORI Masataka, Project Period (FY):1998 - 2000, Research Category:Grant-in-Aid for Scientific Research (B)., Section:展開研究, Research Field:Pathological medical chemistry
LOL...yeah, Rachel is a handful! Just before she swallowed the penny, I had to tell her to stop licking this brick she had brought inside. When she was 2, I caught her eating earth out of the garden with a spoon! No joke. Then there was the day she opened her mouth and a beetle crawled out....and she constantly licks *everything*! The cat, peoples legs, the walls, the counter at the shopping center...I know you guys have heard about her antics before but some days I just want to tie her hands to her sides and put a muzzle on her! I thought she was getting better about it (shes gotten a lot better in general about her sensory issues and activity level) but the last few days weve had some backsliding. Shes not sleeping well...maybe thats whats got her off kilter? Sigh. I would sooooo prefer to not be on a first name basis with the guys at poison control! I will say this much for her...her immune system is amazing. The kid frequently doesnt catch the illnesses her siblings have, and when she ...
On 9 Sep 2003 18:56:56 GMT, bokr at oz.net (Bengt Richter) wrote: ,On 9 Sep 2003 03:59:14 -0700, ben at transversal.com (ben at transversal.com) wrote: , ,,I am slightly confused about the way metaclasses work, having read ,,Metaclass Programming In Python, Parts 1 and 2 ,, ,,I get the fact that the instance of a metaclass is a class, but in ,,this case I fail to see why the following doesnt work: ,, ,,,,, class Meta(type): ,,... def __str__(cls): ,,... print I am + repr(cls) ,,... ,UIAM, you subclassed type, but you didnt override __new__, so ... ARGH. I was was partly mistaken, obviously. ,,,,, ,,,,, Class = Meta(Fish, (), {}) ,became essentially a pass-through call to type, i.e., effectively the same as bzzt. wrong ;-/ It would (I think) have called type.__new__(Meta, Fish, (), {}) , Class = type(Fish,(),{}) ,and since you passed it an empty dict to make its Class.__dict__ from, , The empty dict did become the Class.__dict__ initial value though, I believe. ,,,,, Class.__str__() ...
I do find it a bit annoying. People will chase me down to talk at length about a problem, wanting me to give them advice Im not at all equipped to give and that they wont follow anyway, and just by their speech and their example (I was awake all night thinking about it, I cant stop thinking about it) a non-empath could figure they were anxious about said situation. But I can feel it vibrating unpleasantly in my chest, and finally after standing there, somewhat trapped, kindly listening, etc, I might say, Youre very anxious. And theyll say, crossly, indignantly, No! Im not. Argh. Then I go home vibrating.. Mostly I try to maintain good boundaries and not mention peoples emotional states etc, and just note them to myself. I find writing about it afterwards clarifying and somewhat soothing. I can validate myself nowadays. The days of me wanting to be the worlds Agony Aunt are also over.. I think, in answer to your question, my take on it is it not really your/our business - their ...
On the non-school front, I have been very overwhelmed with so many things going on lately. Soccer frenzy has started, which is fine and fun, but my mind has been so pre-occupied with the planning of a certain 5 year olds birthday party coming up this weekend (which is all I really want to do). And then there is our Disney trip Id really like to sit down and plan all the details for (it is coming up in less than a month and our housing fell through)! And then there are always so many other things we could do. I had to say no to my husbands request to go to a carnival night at the dental conference hes attending this week. A carnival?! Really, I have no time for that! ARGH!!!! The poor man just wants a fun date with his wife...so we are running errands tonight together instead. Yeah. **sigh** I just cant make everyone happy, but I strive to. I am still trying to learn how to say no and live by my priorities (I think this is a life-long one for me ...
L-citrulline plays an important role in human health and nutrition and is an intermediate of the L-arginine biosynthetic pathway. L-citrulline is a by-product of L-arginine production by Corynebacterium glutamicum. In this study, C. glutamicum was engineered for overproduction of L-citrulline as major product without L-arginine being produced as by-product. To this end, L-arginine biosynthesis was derepressed by deletion of the arginine repressor gene argR and conversion of L-citrulline towards L-arginine was avoided by deletion of the argininosuccinate synthetase gene argG. Moreover, to facilitate L-citrulline production the gene encoding a feedback resistant N-acetyl L-glutamate kinase argBfbr as well as the gene encoding L-ornithine carbamoylphosphate transferase argF were overexpressed. The resulting strain accumulated 44.1 ± 0.5 mM L-citrulline from glucose minimal medium with a yield of 0.38 ± 0.01 g⋅g−1 and a volumetric productivity of 0.32 ± 0.01 g⋅l−1⋅h−1. In addition, production
Clinically affected; elevated orotate after allopurinol challenge with absence of argininosuccinic acid (elevated orotic acid = 759); past hyperammonemic events; on citrulline arginine supplement, sodium benzoate and sodium phenylbutyrate; protein restriction; neurologic evaluation was normal at age 1 year; donor subject is heterozygous for a C>T transition at nucleotide 67 in exon 1 of the OTC gene (67C>T) resulting in a stop at codon 23 [Arg23Ter (R23X)] ...
I decided to not call the CBC back after their request to talk about the Gas Station cats. One person made a comment on that blog post that really resonated with me. Last time I had any major publicity I didnt end up with donations or adoptions, but dozens and dozens of people wanting to dump their cats. I thought about going on a local programme "Animal Housecalls". Ive done it before, but never had any more adoptions or donations - just people begging for help. In fact, after that little blip in the local newspaper, I had 6 phone calls today from people wanting help with their cats - and I dont even take cats from the public. Argh ...
Azi a fost pe bază de Wikileaks, peste tot unde te uitai. Nu mai pot cu Wikileaks! M-am trezit azi la 7, am deschis televizorul şi din el ieşeau nişte scursuri de pe Wikileaks de cu o seară înainte... în sinea mea: Oh, boy! Ajung, într-un târziu, la redacţie, deschid... ce e de deschis şi, din nou, ţâşnesc nişte zoaie de Wikileaks pe mine. Oh, crap! Toată ziua am înotat printre aluviunile scurse de pe Wikileaks. Fine way to spend a Monday, you might think! News alert/Breaking news/Whatever news: Wikileaks: Cerul e albastru, iarba e verde, Ricky Martin e gay! Argh! Make it stop! Wikileaks ştie totul despre absolut tot (+ dedesupturile unor experimente NASA pe care încă nu s-a gândit nimeni să le pună în practică.). O migrenă acută mă forţează să închei aici relatarea despre Wikileaks.. END!!!. ...
... If there is one beauty lesson I cannot seem to learn is to remove mascara before doing a look! Argh! I always do th
OH MY GOSH! you are a crazy woman you know! Totally insane! I know, when I feel good I am like a tornado going through and doing stuff I put off! I havent had the total use of my hands since March. This arthritis is absolutely killing me and I guess I will never be the same again. If it were not for this arthritis in my hands I would feel like a million bucks. 50 something SUCKS and I cant imagine what 60 PLUS will be like. I just hope and pray that there is a new drug that will make me ME again! ARGH OH BTW Huhoney is really doing so well. His attitude is amazing and after a few more visits with doctors (heart and genetics) he will be at his best again! lol ...
But in psychiatry, ppl gila 1,..its up to you to describe the symptoms using words how gila they are, wad they say normal onot, but im sure there are many sane ppl that can do things that crazy ppl do...n how u perceive the patients behaviour.. argh, reminds me of the subject i paling benci - Theory of Knowledge... (all the phylosophycal stuff... which i mati also tak faham ...
Homework: The Punnet squares show the enzymatic [colour] and growth [+ or -] phenotypes of two crosses between two dihybrid auxotrophic mutants Assume dominance of the wild-type allele at each locus (x+). What general principles can be drawn from the patterns, as related to tri-hybrid crosses, and other forms of gene interactions in metabolic pathways ...
ACTON, Mass., Jan. 24, 2012 /PRNewswire/ -- Psychemedics Awarded Patent Fundamental To Hair Analysis Drug Testing. The worlds largest drug testing company...
Adrenalectomized and intact rats were given constant high-dose infusions of glucagon, 0.3 mg/kg per day for 7 days, with or without low-dose dexamethasone, 0.01 mg/kg daily, to test whether glucocorticoids potentiate glucagon induction of the 5 urea
Argh. My only option was to cancel Mondays procedure. After last weeks call to the insurance companys 24 hour nurse line, I received a call from a nurse case manager. She explained that the company offers this service to ALL their subscribers for free, but its not widely known that it is available. Anyone can ask for one to be provided. She said shell check in with me periodically to discuss my care options and answer questions. When she called me the other day, I was driving and unable to write her phone number down. She said she will call me sometime this week to follow up and make sure I get her number. When she calls, Ill ask about this ...
Oh God! Theres that word again! Patience! I often say that my stroke was Gods way of teaching me patience. ARGH! Im a child. I want it yesterday! I want it now! I guess I still havent learned that lessons too well yet. Its a work in progress still after almost three years. Meditation, Jo. Breathe. Focus. Breathe. There Im better. No, Im not making fun of meditation. It really works. Ah well, if nothing else I can control my focus and breathing. As for now, Ill leave my leg ataxia on the back burner. I have more pressing issues ...
Argh. I am feeling really annoyed. This morning, my usually chipper, happy, outgoing older daughter didnt want to go to school. It is Tuesday, so it isnt part of her sleeping late on Mondays and Fridays thing. I asked her about it and kept pushing, not satisfied with her mumbled, "I just dont feel like it," answers. Finally it came out. She was nervous about some bullies. A few months ago, one of them punched her. Her school handled it fairly very well. The vice principal herself stepped in and mediated a discussion between Sofi and the bully, and promised to keep an eye on the situation. Sofi, at the time, said she felt the vice principal took her seriously and she was satisfied with how it was handled. But she is still nervous. That punch really shook her. It shook me. She is in first grade and should not be worried about getting punched at school. She also said those bullies - who are in second grade - have been teasing her about her new shoes. She is a very active girl who loves running ...
Thanks to our resident Princess, around here my eReader is known as Miss Nook. Over time, Ive saved my pennies to upgrade and then to upgrade again and have never been disappointed; on the rare occasion that Ive actually spoken with the folks in the "Argh . . . I need help" department of the company regarding some complexity Ive overlooked or misunderstood, about the only comment of note that Ive received is, "Dont you know you can archive books?" [This because the gentleman was suggesting I reset Miss Nook to the factory settings to solve an issue with my book opening but the displayed page being blank. I, of course, was reluctant to do this because I would then have to reshelve the 1.500 or so volumes residing there. Fortunately for both of us, the issue resolved itself and I was saved that particular bit of aggravation.] During the recent hurricane, Miss Nook kept me reasonably sane by allowing me to read despite the loss of power [until the battery ran too low ...
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PROTOCOL OUTLINE: This protocol describes several clinical studies of pharmacologic and dietary management in patients with urea cycle disorders.. Patients with carbamyl phosphate synthetase and ornithine transcarbamylase deficiency are treated with a low-protein diet, essential amino acids (for neonatal onset disease), caloric supplementation, oral sodium phenylbutyrate (now approved as a prescription drug 11/97), and citrulline or arginine free base.. Patients with argininosuccinic acid synthetase deficiency are treated with a low-protein diet, caloric supplementation, oral sodium phenylbutyrate (now approved as a prescription drug 11/97), and arginine free base.. Patients with argininosuccinic aciduria (AA) are treated with a low-protein diet, caloric supplementation, and arginine free base. (Discontinued 11/97) Any patient who develops hyperammonemia is treated with intravenous sodium benzoate, sodium phenylbutyrate, and arginine hydrochloride; benzoate and phenylbutyrate are not given to ...
Figure 5 An approach to diagnosis of hyperammonemia in older children OA: organic acidurias, FAO: fatty acid oxidation defects, PC: pyruvate carboxylase deficiency, PDH: pyruvate dehydrogenase deficiency, ASA: argininosuccinic acid, AS: argininosuccinic aciduria, NAGS: N-acetylglutamate synthetase deficiency, CPS I: carbamoyl phosphate synthetase I deficiency, OTC: ornithine transcarbamoylase deficiency, HHH: hyperornithinemia hyperammonemia homocitrullinuria syndrome, LPI: lysinuric protein intolerance. (Click image to enlarge) ...
After acquiring a degree from Vassar College in psychology, I moved to Western Mass where I ran a peer mentoring network for disabled college students as well as activism and organizing around disability issues. I also conducted research on disabled womens body image. An Upstate New York native, I eventually followed my heliotropic nature to the sun of Southern California. I divide my time between writing (disability fiction and essays) along with moderating San Diego Bisexual Forum which is one of the oldest groups of its kind in the country. In my off hours I can often be found in my neighborhood live music venue enjoying our local talent ...
But, yang tak best nye, even beta-HCG dah drop to normal, I still have to complete my chemo course that would up til 6 cycle. I just finished 4, another 2 to go : bile dah baik ni (sort of dah baik la), macam malas je nak buat chemo.. argh... 2 cycle = 20 days = 10 days to be pricked. ...
Hey all, Sorry for the bother, thanks for the help. Im trying to write a password guessing program to keep track of how many times the user has entered the password wrong. If it is more than 3 times, print ``That must have been complicated. Following is what I got. If I type unicorn it goes straight to welcome in. Great. But after my third mistake, it just loops in That must have been complicated. Id like for someone to tell me why i screwed up. Not to just fix it. Thank you so much in advance. And to give you a little smile for a Monday, Ive been working on this for days....argh #first of all, why does this have to be here? password=foobar count=3 current_count=0 while password !=unicorn: if current_count,count: password=raw_input(Password:) current_count=current_count+1 else: print That must have been complicated print Welcome in Best, Gary Gary Laevsky, Ph.D. Keck Facility Manager, CenSSIS Northeastern University 302 Stearns 360 Huntington Ave. Boston, MA 02115 ...
Argh! I have not been doing much here, as I have not been doing much! I have been staying put at home and tending to all the typical "spring" things, that just are not too exciting. I have not taken many photos lately either. Im not burned out or such, but there just isnt that much to photograph!. Saturday afternoon, we drove the kids down to Bath for an "event" featuring eight different bands. The show began in the late afternoon and ran on until late evening. We dropped the kids off and then went to see Eunice and Noah. I have not seen Eunice in a couple of months, so this was a highlight of my day!. Little Susanna has grown so much! She will celebrated her nine month markthe 17th and I am thinking she must weigh about fourteen pounds now. Pretty good for one who began life under two pounds! She smiles and giggles like crazy and it so adorable in a little white bonnet she wears. Eunice said Susanna did spend five days in the hospital in late February after having a bad cough and breathing ...
Mom! Dont you see Im dying from starvation here?" whined Celestile, the Baby Shoyru. "Huh?" Eileen, Celestiles owner, looked up from playing Feed Florg. "Oh yeah, you dont look too well. Um, hold on; let me finish this game first.". Celestile groaned. She wondered why Eileen would choose an ugly avatar over her, the cute, famous Baby Shoyru. She even had a book with her face on the cover called The Baby Shoyru Book. Celestile rolled her eyes and waited impatiently. Finally, Eileen was done with the game, but as disgruntled at how she missed the avatar by 2 points. "Argh! If only Florg had only eaten a bit sooner! Then, the Warf wouldnt have--" "MOM! IM STARVING!" Celestile cried in her highest pitched voice.. "Oh. Right, sorry. Ill look in my Safety Deposit Box. I still have all that junk from getting the SDB avatar. Theres bound to be some food in there," she replied.. Celestile rolled her eyes again. Another avatar. But at least she was getting somewhere with that empty stomach. ...
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L-Citrulline (1200mg) information including description from Now, supplement facts, and suggested use. Order online for quick delivery at the best prices.
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[70 Pages Report] Check for Discount on Urea Cycle Disorders - Market Insights, Epidemiology and Market Forecast - 2025 report by Delve Insight. DelveInsight s Urea Cycle Disorders - Market Insights, Epidemiology and Market...
Genetic testing for up to 15 genes that encode the enzymes and transporter proteins involved in the biochemical reactions of the urea cycle.
Cytonet announced today that new research on its investigational liver cell therapy (LCT) was presented on July 27, 2014 at the 2014 World Transplant
Decapitated Dan: ARGH!!!!!!!! I have waited over 3 years, yes 3 years, for Volume 3 of Dreamkeepers to come out and now you are telling me there will be a Volume 4! ARGH!!!!! Hands down, this is one of the greatest comics I have ever read. Waiting for book 3 to come out was agony, because I was being deprived of something so good. Starting with the artwork.... ugh it is so BOO-Tiful! I cant even try to explain how amazing the colors are, they seriously make my eyes melt, and speaking of eyes, how amazing did the "main" villain look in this issue. I am so in love with this books visuals. Now I cant lie about the story. I did need to go grab books 1 and 2 and give them a quick glance to catch up, so maybe a recap page is needed, I dont know. But after diving into this book I was fully invested for the next 2 hours, I could no put it down. Dave and Liz crafted this story that grabbed me, and would not let go. The pacing, the dialogue, the concept... it is all so damn good. The only thing I can ...
Im trying desperately not to think that my maternity leave is just about half over (sob!) because right now Im just not ready to go back to that chaos. I am seriously considering cutting my schedule as much as I can and cutting back deliveries for a while until we adjust to the two child family. There is nothing I can do about the call, unfortunately. Also looming on the horizon is the Oral Boards. Argh. I dont even want to think about this. Thank you all for your congratulations and compliments on the Bean. You guys are the best, and I promise that I will get back to more witty, interesting posts as I become more witty and interesting. I am starting to think this is directly proportional to the amount of continuous sleep that I get. Back to the laundry for now ...
Guenter Geiger (Debian/GNU) ,[email protected], realtime-lsm Peter De Schrijver (p2) ,[email protected], linux-atm Stefan Hornburg (Racke) ,[email protected], courier courier-authlib interchange pure-ftpd sympa Cyril Lacoux (Yack) ,[email protected], digitools Marco Presi (Zufus) ,[email protected], linesrv Stefan Alfredsson ,[email protected], monit Osamu Aoki ,[email protected], tpconfig Ben Armstrong ,[email protected], xpilot-ng Don Armstrong ,[email protected], spamass-milter SZALAY Attila ,[email protected], zorp Artem Baguinski ,[email protected], drscheme (U) Alan Bain ,[email protected], rbootd Andreas Barth ,[email protected], mgetty Daniel Baumann ,[email protected], ipmasq nfs-user-server Hilko Bengen ,[email protected], ulog-acctd Grzegorz Bizon ,[email protected], specter Bastian Blank ,[email protected], lvm2 (U) Blars Blarson ,[email protected], cnews Achim Bohnet ,[email protected], capisuite (U) Ed Boraas ,[email protected], aime tinyproxy Cyril Bouthors ,[email protected], bld drbdlinks ...
In maize kernels, mutations in the gene sugary1 (su1) result in (1) increased sucrose concentration; (2) decreased concentration of amylopectin, the branched component of starch; and (3) accumulation of the highly branched glucopolysaccharide phytoglycogen. To investigate further the me...read more ...
Asklepion is developing intravenous formulation of L-citrulline a non-essential amino acid, for prevention of acute post-operative lung injury. L-citrulline is
Nagamani SC, Erez A, Lee B (May 2012). "Argininosuccinate lyase deficiency". Genetics in Medicine. 14 (5): 501-7. doi:10.1038/ ...
"Gene sharing by delta-crystallin and argininosuccinate lyase". Proceedings of the National Academy of Sciences of the United ...
In argininosuccinic aciduria, the enzyme argininosuccinate lyase, involved in the conversion of arginino succinate to arginine ... "Detection of neonatal argininosuccinate lyase deficiency by serum tandem mass spectrometry". J Inherit Metab Dis. 24 (3): 370-8 ...
... is a precursor to fumarate in the citric acid cycle via argininosuccinate lyase. Argininosuccinate ... The enzyme that catalyzes the reaction is argininosuccinate synthetase. ...
... and argininosuccinate lyase (ASL). This is an energetically costly process, because for each molecule of argininosuccinate that ... from citrulline in arginine and proline metabolism by the sequential action of the cytosolic enzymes argininosuccinate ...
... and argininosuccinate lyase (ASL). https://www.nature.com/articles/ncomms15267 Lehninger, Albert L.; Nelson, David L.; Cox, ... is then synthesized from citrulline in the urea cycle by the sequential action of the cytosolic enzymes argininosuccinate ...
1 carbamoyl phosphate synthetase I OTC Ornithine transcarbamoylase ASS argininosuccinate synthetase ASL argininosuccinate lyase ... This reaction is ATP dependent and is catalyzed by argininosuccinate synthetase. 3) Argininosuccinate undergoes cleavage by ... The reactions of the urea cycle 1 L-ornithine 2 carbamoyl phosphate 3 L-citrulline 4 argininosuccinate 5 fumarate 6 L-arginine ... Deficiency of argininosuccinic acid lyase) Argininemia (Deficiency of arginase) Hyperornithinemia, hyperammonemia, ...
... with those from birds and reptiles related to lactate dehydrogenase and argininosuccinate lyase, those of mammals to alcohol ...
Argininosuccinate synthetase and argininosuccinate lyase recycle citrulline, a byproduct of nitric oxide production, into ... Argininosuccinate synthase or synthetase (ASS; EC 6.3.4.5) is an enzyme that catalyzes the synthesis of argininosuccinate from ... Expression of argininosuccinate synthetase in the intestines ceases after two to three years of life. It is thought that ... An argininosuccinate synthetase structure with a bound ATP in the active site has not been attained, although modeling suggests ...
Disorders Overview GeneReviews/NCBI/NIH/UW entry on Argininosuccinate Lyase Deficiency OMIM entries on Argininosuccinate Lyase ... Argininosuccinate lyase is an intermediate enzyme in the urea synthesis pathway and its function is imperative to the ... ASL (argininosuccinate lyase, also known as argininosuccinase) is an enzyme that catalyzes the reversible breakdown of ... Turner MA, Simpson A, McInnes RR, Howell PL (August 1997). "Human argininosuccinate lyase: a structural basis for intragenic ...
... argininosuccinate lyase), which catalyzes the formation of arginine and fumarate from argininosuccinate, the last step in the ... Aspartate ammonia-lyase, EC 4.3.1.1 (aspartase), which catalyzes the reversible conversion of aspartate to fumarate and ammonia ... A number of enzymes, belonging to the lyase class, for which fumarate is a substrate, have been shown to share a short ... Delta-crystallin shares around 90% sequence identity with arginosuccinate lyase, showing that it is an example of a 'hijacked' ...
... amidine-lyases MeSH D08.811.520.232.300.200 --- adenylosuccinate lyase MeSH D08.811.520.232.300.400 --- argininosuccinate lyase ... oxo-acid-lyases MeSH D08.811.520.224.600.200 --- anthranilate synthase MeSH D08.811.520.224.600.700 --- isocitrate lyase MeSH ... ethanolamine ammonia-lyase MeSH D08.811.520.232.400.500 --- histidine ammonia-lyase MeSH D08.811.520.232.400.600 --- l-serine ... lyase MeSH D08.811.520.241.300 --- hydro-lyases MeSH D08.811.520.241.300.050 --- aconitate hydratase MeSH D08.811.520.241. ...
... argininosuccinate lyase EC 4.3.2.2: adenylosuccinate lyase EC 4.3.2.3: ureidoglycolate lyase EC 4.3.2.4: purine imidazole-ring ... pectin lyase EC 4.2.2.11: poly(a-L-guluronate) lyase EC 4.2.2.12: xanthan lyase EC 4.2.2.13: exo-(1-4)-α-D-glucan lyase EC 4.2. ... pectate lyase EC 4.2.2.3: poly(b-D-mannuronate) lyase EC 4.2.2.4: chondroitin ABC lyase EC 4.2.2.5: chondroitin AC lyase EC 4.2 ... oligogalacturonide lyase EC 4.2.2.7: heparin lyase EC 4.2.2.8: heparin-sulfate lyase EC 4.2.2.9: pectate disaccharide-lyase EC ...
EC 4.3.1 Phenylalanine ammonia-lyase (EC 4.3.1.24) Category:EC 4.4.1 Cystathionine gamma-lyase Cystathionine beta-lyase ... Argininosuccinate synthetase (EC 6.3.4.5) CTP synthase (EC 6.3.4.2) Pyruvate carboxylase (EC 6.4.1.1) Acetyl-CoA carboxylase ( ...
It is also said that a synthase is a lyase (a lyase is an enzyme that catalyzes the breaking of various chemical bonds by means ... including argininosuccinate synthetase) EC 6.4 includes ligases used to form carbon-carbon bonds EC 6.5 includes ligases used ...
The following reaction step, catalyzed by the enzyme O-acetyl serine (thiol) lyase, replaces the acetyl group of O-acetyl-L- ... Then, the enzymes citrulline and argininosuccinate convert ornithine to arginine. There are two distinct lysine biosynthetic ... SAICAR lyase removes the carbon skeleton of the added aspartate, leaving the amino group and forming 5-aminoimidazole-4- ...
... is a rare disease caused by a deficiency in argininosuccinate synthetase, an enzyme involved in excreting excess nitrogen from ... argininosuccinic acid lyase deficiency, ornithine transcarbamylase deficiency, arginase deficiency, and N-Acetylglutamate ...
... lyase) ligase EC 6.2.1.23: dicarboxylate-CoA ligase EC 6.2.1.24: phytanate-CoA ligase EC 6.2.1.25: benzoate-CoA ligase EC 6.2. ... argininosuccinate synthase EC 6.3.4.6: urea carboxylase EC 6.3.4.7: ribose-5-phosphate-ammonia ligase EC 6.3.4.8: ...
Argininosuccinate synthase. *Holocarboxylase synthetase. *GMP synthase. *Asparagine synthetase. *Carbamoyl phosphate synthetase ...
Argininosuccinate synthase. *Holocarboxylase synthetase. *GMP synthase. *Asparagine synthetase. *Carbamoyl phosphate synthetase ...
EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list). *EC7 Translocases (list) ... Argininosuccinate synthase. *Holocarboxylase synthetase. *GMP synthase. *Asparagine synthetase. *Carbamoyl phosphate synthetase ...
... anemia due to Adenylosuccinate lyase deficiency Adie syndrome Adiposis dolorosa, aka Dercum's disease Adolescent benign focal ... II Arbovirosis Arc syndrome AREDYLD syndrome Argentine hemorrhagic fever Arginase deficiency Arginemia Argininosuccinate ...
Argininosuccinate synthase. *Holocarboxylase synthetase. *GMP synthase. *Asparagine synthetase. *Carbamoyl phosphate synthetase ...
Category:Lyases (EC 4) (Lyase)Edit. Category:EC 4.1 (carbon-carbon lyases)Edit. *Category:EC 4.1.1 *Ornithine decarboxylase (EC ... Argininosuccinate synthetase (EC 6.3.4.5). *CTP synthase (EC 6.3.4.2). Category:EC 6.4 (form carbon-carbon bonds)Edit. * ... 4 Category:Lyases (EC 4) (Lyase) *4.1 Category:EC 4.1 (carbon-carbon lyases) ... Category:EC 4.3 (carbon-nitrogen lyases)Edit. *Category:EC 4.3.1 *Phenylalanine ammonia-lyase (EC 4.3.1.24) ...
2-hydroxyphytanoyl-CoA lyase. *2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase ... Argininosuccinate synthase. *Argonaute. *Argos (EGFR Inhibitor). *Aromatic L-amino acid decarboxylase. *Arrestin ...
Argininosuccinate lyase deficiency causes an endothelial-dependent form of hypertension. Am J Hum Genet. 2018 Aug 2;103(2):276- ... Alterations in the ASL gene lead to low levels of functional argininosuccinate lyase, which is needed to break down nitrogen in ... Nagamani SCS, Erez A, Lee B. Argininosuccinate Lyase Deficiency. 2011 Feb 3 [Updated 2012 Feb 2]. In: Adam MP, Ardinger HH, ... Symptoms of argininosuccinic aciduria develop due to the near complete or partial lack of the enzyme argininosuccinate lyase. ...
Disorders Overview GeneReviews/NCBI/NIH/UW entry on Argininosuccinate Lyase Deficiency OMIM entries on Argininosuccinate Lyase ... Argininosuccinate lyase is an intermediate enzyme in the urea synthesis pathway and its function is imperative to the ... ASL (argininosuccinate lyase, also known as argininosuccinase) is an enzyme that catalyzes the reversible breakdown of ... Turner MA, Simpson A, McInnes RR, Howell PL (August 1997). "Human argininosuccinate lyase: a structural basis for intragenic ...
IPR009049 Argininosuccinate_lyase. IPR024083 Fumarase/histidase_N. IPR020557 Fumarate_lyase_CS. IPR000362 Fumarate_lyase_fam. ... IPR009049 Argininosuccinate_lyase. IPR024083 Fumarase/histidase_N. IPR020557 Fumarate_lyase_CS. IPR000362 Fumarate_lyase_fam. ... Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.UniRule annotation. Manual assertion according to rulesi ... Argininosuccinate lyase (argH). This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino- ...
Molecular cloning of the ARG7 gene of Schizosaccharomyces pombe encoding argininosuccinate lyase. *Jacques E. Remacle1. , ... One colony was recovered which contained the YEp13 plasmid bearing a large insert complementing the argininosuccinate lyase ( ... Molecular cloning of the ARG7 gene of Schizosaccharomyces pombe encoding argininosuccinate lyase. Curr Genet 14, 381-385 (1988 ...
Epigenetic status of argininosuccinate synthetase and argininosuccinate lyase modulates autophagy and cell death in ... 2013) Epigenetic status of argininosuccinate synthetase and argininosuccinate lyase modulates autophagy and cell death in ...
... is caused by a defect of the urea cycle enzyme argininosuccinate lyase (ASL) encoded by the ASL gene. Patients often present ... Argininosuccinate lyase deficiency (ASLD) is caused by a defect of the urea cycle enzyme argininosuccinate lyase (ASL) encoded ... Low prevalence of argininosuccinate lyase deficiency among inherited urea cycle disorders in Korea. Dahye Kim, Jung Min Ko, +4 ... Cloning and sequence analysis of cDNA for human argininosuccinate lyase.. William E Obrien, Roderick R Mcinnes, K. E. Kalumuck ...
This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes ... Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1. 1 ... Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase ... the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in ...
Requirement of argininosuccinate lyase for systemic nitric oxide production. In: Nature Medicine. 2011 ; Vol. 17, No. 12. pp. ... Requirement of argininosuccinate lyase for systemic nitric oxide production. Nature Medicine. 2011 Dec 1;17(12):1619-1626. ... Requirement of argininosuccinate lyase for systemic nitric oxide production. Ayelet Erez, Sandesh C.S. Nagamani, Oleg A. ... Requirement of argininosuccinate lyase for systemic nitric oxide production. / Erez, Ayelet; Nagamani, Sandesh C.S.; ...
Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287 ...
Caulobacter crescentus Argininosuccinate lyase (argH) datasheet and description hight quality product and Backed by our ... Caulobacter crescentus Argininosuccinate lyase (argH). Alternative name: Caulobacter crescentus Argininosuccinate lyase (argH) ... Recombinant Caulobacter crescentus Argininosuccinate lyase (argH). Alternative names: Argininosuccinate lyase, Arginosuccinase ... Argininosuccinate lyase(argH) is a recombinant protein expressed in Yeast ...
Argininosuccinase acid lyase explanation free. What is Argininosuccinase acid lyase? Meaning of Argininosuccinase acid lyase ... Looking for online definition of Argininosuccinase acid lyase in the Medical Dictionary? ... argininosuccinate lyase. (redirected from Argininosuccinase acid lyase) ar·gi·ni·no·suc·ci·nate ly·ase. (arji-ni-nō-sŭksi-nāt ... a href=https://medical-dictionary.thefreedictionary.com/Argininosuccinase+acid+lyase,argininosuccinate lyase,/a,. *Facebook ...
Argininosuccinate lyase is an essential regulator of systemic nitric oxide produc Lee, Brendan Hl Baylor College of Medicine, ... Argininosuccinate lyase is an essential regulator of systemic nitric oxide produc. Lee, Brendan / Baylor College of Medicine. ... Argininosuccinate lyase is an essential regulator of systemic nitric oxide produc. Lee, Brendan / Baylor College of Medicine. ... Argininosuccinate lyase is an essential regulator of systemic nitric oxide produc. Lee, Brendan / Baylor College of Medicine. ...
argininosuccinate lyase. ASS. argininosuccinate synthase. CPA. N-carbamoylputrescine amidohydrolase. CPS. carbamoylsynthetase. ...
Argininosuccinate lyase. Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) ... Argininosuccinate lyase UniProtKBInterProInteractive Modelling. 457 aa; Sequence (Fasta) Identical sequences: Shigella flexneri ...
ASL deficiency -- Argininosuccinate lyase. *ASS1 deficiency -- Argininosuccinate synthase 1. *Citrin deficiency -- Citrin ...
Review Argininosuccinate Lyase Deficiency[GeneReviews®. 1993]. Review Argininosuccinate Lyase Deficiency. Nagamani SCS, Erez A ...
... which is then taken up into the kidney and converted by argininosuccinate synthase (ASS) and argininosuccinate lyase (ASL) into ... Argininosuccinate lyase (ASL) enzyme was also down-regulated. The kidney plays a key role in arginine metabolism. Renal l- ...
Argininosuccinate Lyase Deficiency Urea Cycle Disorder Urea Cycle Disorders, Inborn Argininosuccinic Aciduria Dietary ... Patients with argininosuccinate lyase deficiency (ASLD) will be randomly assigned to receive either a nitric oxide dietary ... Argininosuccinate lyase deficiency (ASLD; also known as argininosuccinic aciduria) is the second most common urea cycle ... Nagamani SCS, Erez A, Lee B. Argininosuccinate Lyase Deficiency. 2011 Feb 3 [updated 2012 Feb 2]. In: Adam MP, Ardinger HH, ...
Hung, Y. H., Huang, H. L., Chen, W. C., Yen, M. C., Cho, C. Y., Weng, T. Y., ... Lai, M. D. (2017). Argininosuccinate lyase ... Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells. In: Oncology Reports. ... Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells. Oncology Reports. 2017 ... Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells. / Hung, Yu Hsuan; ...
Review Argininosuccinate Lyase Deficiency[GeneReviews®. 1993]. Review Argininosuccinate Lyase Deficiency. Nagamani SCS, Erez A ... Argininosuccinate synthase enzyme activity can be measured in uncultured fetal tissue obtained by chorionic villus sampling ( ... Abnormal gene product. The argininosuccinate synthase enzyme is inactive or absent. Mutated ASS with abnormal KM (Michaelis ... Argininosuccinate synthase (ASS) enzyme activity. Incorporation of radiolabeled citrulline into argininosuccinic acid is ...
Argininosuccinate Lyase (ASL) Deficiency * Carbamoyl Phosphate Synthetase (CPS) Deficiency * N-Acetylglutamate Synthetase ... Compound 5 is fumaric acid generated in the reaction that converts ASA to arginine (6), which is mediated by ASA lyase. ...
... argininosuccinate synthetase deficiency (citrullinemia); argininosuccinate lyase (ASL) deficiency; arginase deficiency ( ...
Argininosuccinate lyase. Serum. 0-4 U/dL. 10. 0-40 U/L. ...
Argininosuccinate Lyase 4.3.2.1. (R)-Aromatic Alcohol Dehydrogenase, NADP+ Dependent (EC 1.1.1.2) 1.1.1.2. ...
ASL: argininosuccinate lyase. *ASPA: aspartoacylase. *ASPM: abnormal spindle microtubule assembly. *ASS1: argininosuccinate ...
  • A number of enzymes, belonging to the lyase class, for which fumarate is a substrate, have been shown to share a short conserved sequence around a methionine which is probably involved in the catalytic activity of this type of enzymes [ PMID: 3282546 ]. (ebi.ac.uk)
  • Arginine is synthesized from citrulline in arginine and proline metabolism by the sequential action of the cytosolic enzymes argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL). (amazonaws.com)
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Argininosuccinate Lyase(GTX113629) antibody at 1:200 dilution. (genetex.com)
  • Both ASS 1 and argininosuccinate lyase (ASL) are down-regulated in glioblastoma multiforme (GBM). (drugs.com)