An enzyme that catalyzes the phosphorylation of the guanidine nitrogen of arginine in the presence of ATP and a divalent cation with formation of phosphorylarginine and ADP. EC 2.7.3.3.
Family of large marine CRUSTACEA, in the order DECAPODA. These are called clawed lobsters because they bear pincers on the first three pairs of legs. The American lobster and Cape lobster in the genus Homarus are commonly used for food.
A class of Echinodermata characterized by long, slender bodies.
A transferase that catalyzes formation of PHOSPHOCREATINE from ATP + CREATINE. The reaction stores ATP energy as phosphocreatine. Three cytoplasmic ISOENZYMES have been identified in human tissues: the MM type from SKELETAL MUSCLE, the MB type from myocardial tissue and the BB type from nervous tissue as well as a mitochondrial isoenzyme. Macro-creatine kinase refers to creatine kinase complexed with other serum proteins.
An arthropod subclass (Xiphosura) comprising the North American (Limulus) and Asiatic (Tachypleus) genera of horseshoe crabs.
A phylum of the most familiar marine invertebrates. Its class Stelleroidea contains two subclasses, the Asteroidea (the STARFISH or sea stars) and the Ophiuroidea (the brittle stars, also called basket stars and serpent stars). There are 1500 described species of STARFISH found throughout the world. The second class, Echinoidea, contains about 950 species of SEA URCHINS, heart urchins, and sand dollars. A third class, Holothuroidea, comprises about 900 echinoderms known as SEA CUCUMBERS. Echinoderms are used extensively in biological research. (From Barnes, Invertebrate Zoology, 5th ed, pp773-826)
A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.
A hydrated form of silicon dioxide. It is commonly used in the manufacture of TOOTHPASTES and as a stationary phase for CHROMATOGRAPHY.
A superorder in the class CEPHALOPODA, consisting of the orders Octopoda (octopus) with over 200 species and Vampyromorpha with a single species. The latter is a phylogenetic relic but holds the key to the origins of Octopoda.
Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.
An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
An essential amino acid that is physiologically active in the L-form.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The method of measuring the dispersion of an optically active molecule to determine the relative magnitude of right- or left-handed components and sometimes structural features of the molecule.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
An infraorder of chiefly marine, largely carnivorous CRUSTACEA, in the order DECAPODA, including the genera Cancer, Uca, and Callinectes.
A phylum of the kingdom Metazoa. Mollusca have soft, unsegmented bodies with an anterior head, a dorsal visceral mass, and a ventral foot. Most are encased in a protective calcareous shell. It includes the classes GASTROPODA; BIVALVIA; CEPHALOPODA; Aplacophora; Scaphopoda; Polyplacophora; and Monoplacophora.
A group of enzymes that catalyzes the transfer of a phosphate group onto a nitrogenous group acceptor. EC 2.7.3.
Agents that inhibit PROTEIN KINASES.
A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. This enzyme is also sometimes dependent on CALCIUM. A wide range of proteins can act as acceptor, including VIMENTIN; SYNAPSINS; GLYCOGEN SYNTHASE; MYOSIN LIGHT CHAINS; and the MICROTUBULE-ASSOCIATED PROTEINS. (From Enzyme Nomenclature, 1992, p277)
The rate dynamics in chemical or physical systems.
The predominant form of mammalian antidiuretic hormone. It is a nonapeptide containing an ARGININE at residue 8 and two disulfide-linked cysteines at residues of 1 and 6. Arg-vasopressin is used to treat DIABETES INSIPIDUS or to improve vasomotor tone and BLOOD PRESSURE.
A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A class in the phylum MOLLUSCA comprised of mussels; clams; OYSTERS; COCKLES; and SCALLOPS. They are characterized by a bilaterally symmetrical hinged shell and a muscular foot used for burrowing and anchoring.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
Organic compounds that contain phosphorus as an integral part of the molecule. Included under this heading is broad array of synthetic compounds that are used as PESTICIDES and DRUGS.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A mitogen-activated protein kinase subfamily that regulates a variety of cellular processes including CELL GROWTH PROCESSES; CELL DIFFERENTIATION; APOPTOSIS; and cellular responses to INFLAMMATION. The P38 MAP kinases are regulated by CYTOKINE RECEPTORS and can be activated in response to bacterial pathogens.
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A class of enzymes that transfers phosphate groups and has a carboxyl group as an acceptor. EC 2.7.2.
A mitochondrial matrix enzyme that catalyzes the synthesis of L-GLUTAMATE to N-acetyl-L-glutamate in the presence of ACETYL-COA.
Oxidoreductases that are specific for ALDEHYDES.
A genus of ascomycetous fungi, family Sordariaceae, order SORDARIALES, comprising bread molds. They are capable of converting tryptophan to nicotinic acid and are used extensively in genetic and enzyme research. (Dorland, 27th ed)
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
The portion of an interactive computer program that issues messages to and receives commands from a user.
Sequential operating programs and data which instruct the functioning of a digital computer.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
Paired respiratory organs of fishes and some amphibians that are analogous to lungs. They are richly supplied with blood vessels by which oxygen and carbon dioxide are exchanged directly with the environment.
A primitive form of digestive gland found in marine ARTHROPODS, that contains cells similar to those found in the mammalian liver (HEPATOCYTES), and the PANCREAS.
A phylum of EUKARYOTES in the RHIZARIA group. They are small endoparasites of marine invertebrates. Spores are structurally complex but without polar filaments or tubes.
The blood/lymphlike nutrient fluid of some invertebrates.
Carbonic acid (H2C03). The hypothetical acid of carbon dioxide and water. It exists only in the form of its salts (carbonates), acid salts (hydrogen carbonates), amines (carbamic acid), and acid chlorides (carbonyl chloride). (From Grant & Hackh's Chemical Dictionary, 5th ed)
A genus of large SEA CUCUMBERS in the family Holothuriidae possessing thick body walls, a warty body surface, and microscopic ossicles.
Repetitive withdrawal of small amounts of blood and replacement with donor blood until a large proportion of the blood volume has been exchanged. Used in treatment of fetal erythroblastosis, hepatic coma, sickle cell anemia, disseminated intravascular coagulation, septicemia, burns, thrombotic thrombopenic purpura, and fulminant malaria.

O-Crystallin, arginine kinase and ferritin from the octopus lens. (1/75)

Three proteins have been identified in the eye lens of the octopus, Octopus dofleini. A 22 kDa protein comprising 3-5% of the soluble protein of the lens is 35-43% identical to a family of phosphatidylethanolamine-binding proteins of vertebrates. Other members of this family include the immunodominant antigen of the filarial parasite, Onchocerca volvulus, putative odorant-binding proteins of Drosophila and a protein with unknown function of Caenorhabditis elegans. We have called this protein O-crystallin on the basis of its abundance in the transparent lens. O-Crystallin mRNA was detected only in the lens. Two tryptic peptides of another octopus lens protein, less abundant than O-crystallin, showed 80% identity to arginine kinase of invertebrates, a relative of creatine kinase of vertebrates. Finally, ferritin cDNA was isolated as an abundant cDNA from the octopus lens library. Northern blots showed that ferritin mRNA is not lens-specific.  (+info)

Arginine kinase evolved twice: evidence that echinoderm arginine kinase originated from creatine kinase. (2/75)

Arginine kinase (AK) was isolated from the longitudinal muscle of the sea cucumber Stichopus japonicus. Unlike the monomeric 40 kDa AKs from molluscs and arthropods, but like the cytoplasmic isoenzymes of vertebrate creatine kinase (CK), the Stichopus enzyme was dimeric. To explore the evolutionary origin of the dimeric AK, we determined its cDNA-derived amino acid sequence of 370 residues. A comparison of the sequence with those of other enzymes belonging to the phosphagen kinase family indicated that the entire amino acid sequence of Stichopus AK is apparently much more similar to vertebrate CKs than to all other AKs. A phylogenetic tree also strongly suggests that the Stichopus AK has evolved from CK. These results support the conclusion that AK evolved at least twice during the evolution of phosphagen kinases: first at an early stage of phosphagen kinase evolution (its descendants are molluscan and arthropod AKs) and secondly from CK later in metazoan evolution. A comparison of the amino acid sequence around the guanidino specificity (GS) region (which is a possible candidate for the guanidine substrate recognition site in the phosphagen kinase family) of the Stichopus enzyme with those of other phosphagen kinases showed that the GS region of the Stichopus enzyme was of the AK type: five amino acid deletions in the flexible loop region that might help to accommodate larger guanidine substrates in the active site. The presence of the AK-type deletions in the Stichopus AK, even though it seems that the enzyme's most immediate ancestor was probably CK, strongly suggests that the GS region has a role in substrate specificity. Stichopus AK and presumably other echinoderm AKs seem to have evolved from the CK gene; the sequence of GS region might have been replaced by the AK type via exon shuffling. The presence of an intron near the GS region in the Stichopus AK gene supports this hypothesis.  (+info)

Thermodynamics of the arginine kinase reaction. (3/75)

The effect of temperature, pH, free [Mg(2+)], and ionic strength on the apparent equilibrium constant of arginine kinase (EC 2.7.3.3) was determined. At equilibrium, the apparent K' was defined as [see text] where each reactant represents the sum of all the ionic and metal complex species. The K' at pH 7.0, 1.0 mM free [Mg(2+)], and 0. 25 M ionic strength was 29.91 +/- 0.59, 33.44 +/- 0.46, 35.44 +/- 0. 71, 39.64 +/- 0.74, and 45.19 +/- 0.65 (n = 8) at 40, 33, 25, 15, and 5 degrees C, respectively. The standard apparent enthalpy (DeltaH degrees') is -8.19 kJ mol(-1), and the corresponding standard apparent entropy of the reaction (DeltaS degrees') is + 2. 2 J K(-1)mol(-1) in the direction of ATP formation at pH 7.0, free [Mg(2+)] =1.0 mM, ionic strength (I) =0.25 M at 25 degrees C. We further show that the magnitude of transformed Gibbs energy (DeltaG degrees ') of -8.89 kJ mol(-1) is mostly comprised of the enthalpy of the reaction, with 7.4% coming from the entropy TDeltaS degrees' term (+0.66 kJ mol(-1)). Our results are discussed in relation to the thermodynamic properties of its evolutionary successor, creatine kinase.  (+info)

Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites. (4/75)

This work contains the first description of a guanidino kinase in a flagellar unicellular parasite. The enzyme phosphorylates L-arginine and was characterized in preparations from Trypanosoma cruzi, the ethiological agent of Chagas' disease. The activity requires ATP and a divalent cation. Under standard assay conditions (1 mM L-arginine), the presence of 5-fold higher concentrations of canavanine or histidine produced a greater than 50% enzyme inhibition. The base sequence of this enzyme revealed an open reading frame of 357 amino acids and a molecular weight of 40,201. The amino acid sequence shows all of the characteristic consensus blocks of the ATP:guanidino phosphotransferase family and a putative "actinin-type" actin-binding domain. The highest amino acid identities of the T. cruzi sequence, about 70%, were with arginine kinases from Arthropoda. Southern and chromosome blots revealed that the kinase is encoded by a single-copy gene. Moreover, Northern blot analysis showed an mRNA subpopulation of about 2.0 kilobases, and Western blotting of T. cruzi-soluble polypeptides revealed a 40-kDa band. The finding in the parasite of a phosphagen and its biosynthetic pathway, which are totally different from those in mammalian host tissues, points out this arginine kinase as a possible chemotherapy target for Chagas' disease.  (+info)

Induced fit in arginine kinase. (5/75)

Creatine kinase (CK) and arginine kinase (AK) are related enzymes that reversibly transfer a phosphoryl group between a guanidino compound and ADP. In the buffering of ATP energy levels, they are central to energy metabolism and have been paradigms of classical enzymology. Comparison of the open substrate-free structure of CK and the closed substrate-bound structure of AK reveals differences that are consistent with prior biophysical evidence of substrate-induced conformational changes. Large and small domains undergo a hinged 13 degrees rotation. Several loops become ordered and adopt different positions in the presence of substrate, including one (residues 309-319) that moves 15 A to fold over the substrates. The conformational changes appear to be necessary in aligning the two substrates for catalysis, in configuring the active site only when productive phosphoryl transfer is possible, and excluding water from the active site to avoid wasteful ATP hydrolysis.  (+info)

Noninvasive measurement of gene expression in skeletal muscle. (6/75)

We have developed a noninvasive detection method for expression of viral-mediated gene transfer. A recombinant adenovirus was constructed by using the gene for arginine kinase (AK), which is the invertebrate correlate to the vertebrate ATP-buffering enzyme, creatine kinase. Gene expression was noninvasively monitored using (31)P-magnetic resonance spectroscopy ((31)P-MRS). The product of the AK enzyme, phosphoarginine (PArg), served as an MRS-visible reporter of AK expression. The recombinant adenovirus coding for arginine kinase (rAdCMVAK) was injected into the right hindlimbs of neonatal mice. Two weeks after injection of rAdCMVAK, a unique (31)P-MRS resonance was observed. It was observable in all rAdCMVAK injected hindlimbs and was not present in the contralateral control or the vehicle injected limb. PArg and phosphocreatine (PCr) concentrations were calculated to be 11.6 +/- 0.90 and 13.6 +/- 1.1 mM respectively in rAdCMVAK injected limbs. AK activity was demonstrated in vivo by monitoring the decreases in PArg and ATP resonances during prolonged ischemia. After 1 h of ischemia intracellular pH was 6.73 +/- 0.06, PCr/ATP was decreased by 77 +/- 8%, whereas PArg/ATP was decreased by 50 +/- 15% of basal levels. PArg and PCr returned to basal levels within 5 min of the restoration of blood flow. AK activity persisted for at least 8 mo after injection, indicating that adenoviral-mediated gene transfer can produce stable expression for long periods of time. Therefore, the cDNA encoding AK provides a useful reporter gene that allows noninvasive and repeated monitoring of gene expression after viral mediated gene transfer to muscle.  (+info)

Arginine kinase from Nautilus pompilius, a living fossil. Site-directed mutagenesis studies on the role of amino acid residues in the Guanidino specificity region. (7/75)

Arginine kinases were isolated from the cephalopods Nautilus pompilius, Octopus vulgaris, and Sepioteuthis lessoniana, and the cDNA-derived amino acid sequences have been determined. Although the origin and evolution of cephalopods have long been obscure, this work provides the first molecular evidence for the phylogenetic position of Cephalopoda in molluscan evolution. A crystal structure for Limulus arginine kinase showed that four amino acid residues (Ser(63), Gly(64), Val(65), and Tyr(68)) are hydrogen-bonded with the substrate arginine. We introduced three independent mutations, Ser(63) --> Gly, Ser(63) --> Thr, and Tyr(68) --> Ser, in Nautilus arginine kinase. One of the mutants had a considerably reduced substrate affinity, accompanied by a decreased V(max). In other mutants, the activity was lost almost completely. It is known that substantial conformational changes take place upon substrate binding in arginine kinase. We hypothesize that the hydrogen bond between Asp(62) and Arg(193) stabilizes the closed, substrate-bound state. Site-directed mutagenesis studies strongly support this hypothesis. The mutant (Asp(62) --> Gly or Arg(193) --> Gly), which destabilizes the maintenance of the closed state and/or perhaps disrupts the unique topology of the catalytic pocket, showed only a very weak activity (0.6-1.5% to the wild-type).  (+info)

Expression of arginine kinase enzymatic activity and mRNA in gills of the euryhaline crabs Carcinus maenas and Callinectes sapidus. (8/75)

Phosphagen kinases catalyze the reversible dephosphorylation of guanidino phosphagens such as phosphocreatine and phosphoarginine, contributing to the restoration of adenosine triphosphate concentrations in cells experiencing high and variable demands on their reserves of high-energy phosphates. The major invertebrate phosphagen kinase, arginine kinase, is expressed in the gills of two species of euryhaline crabs, the blue crab Callinectes sapidus and the shore crab Carcinus maenas, in which energy-requiring functions include monovalent ion transport, acid-base balance, nitrogen excretion and gas exchange. The enzymatic activity of arginine kinase approximately doubles in the ion-transporting gills of C. sapidus, a strong osmoregulator, when the crabs are transferred from high to low salinity, but does not change in C. maenas, a more modest osmoregulator. Amplification and sequencing of arginine kinase cDNA from both species, accomplished by reverse transcription of gill mRNA and the polymerase chain reaction, revealed an open reading frame coding for a 357-amino-acid protein. The predicted amino acid sequences showed a minimum of 75 % identity with arginine kinase sequences of other arthropods. Ten of the 11 amino acid residues believed to participate in arginine binding are completely conserved among the arthropod sequences analyzed. An estimation of arginine kinase mRNA abundance indicated that acclimation salinity has no effect on arginine kinase gene transcription. Thus, the observed enhancement of enzyme activity in C. sapidus probably results from altered translation rates or direct activation of pre-existing enzyme protein.  (+info)

TY - JOUR. T1 - Biochemical comparison of arginine kinase allozymes in Drosophila melanogaster. AU - Chien, Yi-Chih. AU - Collier, Glen E.. PY - 1997/10/1. Y1 - 1997/10/1. N2 - Biochemical comparison of arginine kinase allozymes in Drosophila melanogaster. Zoological Studies 36(4): 277-287. ARKB is a rare arginine kinase allozyme found in natural populations of Drosophila melanogaster. To test whether the rarity of this allozyme could be due to its biochemical impairment relative to the common allozyme, biochemical properties such as catalytic efficiency and conformational stability of the rare (ARKB) and the common (ARKA) allozymes were compared in this study. Both allozymes were purified by ammonium sulfate fractionation, DEAE-ion-exchange column, Blue-Sepharose, and S-300 gel filtration, to yield a single coomassie-blue band on SDS-polyacrylamide gels. ARKA has a higher Vmax or Vmax/Km than ARKB at 18 or 29 °C, but there are no differences at 24 °C. In general, ARKA is catalytically more ...
Phosphagen (guanidino) kinases, including creatine kinase (CK), arginine kinase (AK), taurocyamine kinase (TK), lombricine kinase (LK), glycocyamine kinase (GK), and hypotaurocyamine kinase (HTK), are enzymes that catalyze the reversible transfer of the γ-phosphoryl group of adenosine triphosphate (ATP) to naturally occuring guanidino compounds such as creatine, arginine, yelding adenosine diphosphate (ADP) and a phosphorylated guanidine typically referred to as phosphagen (phosphocreatine, phosphoarginine and etc). Members of this enzyme family play a key role in animals as ATP-buffering systems in cells that display high and variable rates of ATP turnover. Phosphagen kinases have been found in all animal species and in some protozoa, such as trypanosomes, choanoflagellates, and the ciliates, Paramecium tetraurelia, Paramecium caudatum, and Tetrahymena. Eukaryotic phosphagen kinases consist of a small, ~100-residue, α-helical N-terminal domain and a larger, 250+-residue, C-terminal α/β ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
AL009126.MCSB Location/Qualifiers FT CDS 102484..103575 FT /codon_start=1 FT /transl_table=11 FT /gene=mcsB FT /locus_tag=BSU_00850 FT /old_locus_tag=BSU00850 FT /product=protein arginine kinase FT /function=16.3: Control FT /EC_number=2.7.3.3 FT /note=Evidence 1a: Function from experimental evidences in FT the studied strain; PubMedId: 16163393, 17380125, 19189946, FT 21622759, 25610436, 27749819; Product type e : enzyme FT /db_xref=EnsemblGenomes-Gn:BSU00850 FT /db_xref=EnsemblGenomes-Tr:CAB11861 FT /db_xref=GOA:P37570 FT /db_xref=InterPro:IPR000749 FT /db_xref=InterPro:IPR014746 FT /db_xref=InterPro:IPR022414 FT /db_xref=InterPro:IPR022415 FT /db_xref=InterPro:IPR023660 FT /db_xref=SubtiList:BG10147 FT /db_xref=UniProtKB/Swiss-Prot:P37570 FT /experiment=publication(s) with functional evidences, FT PMID:16163393, 17380125, 19189946, 21622759, 25610436, FT 27749819 FT /protein_id=CAB11861.1 FT /translation=MSLKHFIQDALSSWMKQKGPESDIVLSSRIRLARNFEHIRFPTRY FT ...
Protein quality control systems are essential for the viability and growth of all living organisms. They protect the cell from irreversible protein aggregation. Because the frequency of protein misfolding, which ultimately results in protein aggregation, varies with the environmental conditions, the amount and activity of protein quality systems have to be accurately adapted to the rate of protein misfolding. The main goal of this thesis was to gain detailed molecular insights into the transcriptional and post-translational regulation of these protein quality control networks in the ecologically, medically and industrially important phylum of low GC, Gram-positive bacteria. In these bacteria the core protein quality control systems are under the transcriptional control of the global repressor CtsR. In a first study it was demonstrated that the arginine kinase McsB is not responsible for the regulation of CtsR activity during heat stress, as was concluded by others on the basis of previous in ...
p23 is a member of the p24 family of proteins. (A) cDNA-derived amino acid sequence of the BHKp23 precursor. The predicted cleavage site for the signal peptid
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Phosphagen kinases catalyze the reversible dephosphorylation of guanidino phosphagens such as phosphocreatine and phosphoarginine, contributing to the restoration of adenosine triphosphate concentrations in cells experiencing high and variable demands on their reserves of high-energy phosphates. The major invertebrate phosphagen kinase, arginine kinase, is expressed in the gills of two species of euryhaline crabs, the blue crab Callinectes sapidus and the shore crab Carcinus maenas, in which energy-requiring functions include monovalent ion transport, acid-base balance, nitrogen excretion and gas exchange. The enzymatic activity of arginine kinase approximately doubles in the ion-transporting gills of C. sapidus, a strong osmoregulator, when the crabs are transferred from high to low salinity, but does not change in C. maenas, a more modest osmoregulator. Amplification and sequencing of arginine kinase cDNA from both species, accomplished by reverse transcription of gill mRNA and the polymerase ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
1. Both the monomer arginine kinase from lobster muscle and the dimer arginine kinase from Holothuria forskali catalyse the ATP-ADP partial exchange reaction at rates equal to 3 and 0.6% of the normal rate of transphosphorylation respectively. The Mg2+-nucleotide complex is the substrate for this as it is for the kinase reaction. 2. Analogues of arginine inhibit the exchange reaction of the lobster enzyme but enhance that of the Holothuria enzyme. 3. With the lobster enzyme NO3- has no effect on the exchange reaction alone and inhibit only slightly the apparent enhancement of the exchange reaction produced by the addition of arginine. This is compatible with previous findings for this enzyme that formation of the anion-stabilized dead-end complex, enzyme-arginine-MgADP-NO3-, does not occur to any marked degree. 4. About 80% of the ADP-ATP exchange reaction of the lobster enzyme remains after inhibition with iodoacetamide. This is further decreased to 65% by the addition of L-arginine, indicating ...
Get information, facts, and pictures about phosphagen at Encyclopedia.com. Make research projects and school reports about phosphagen easy with credible articles from our FREE, online encyclopedia and dictionary.
TY - JOUR. T1 - Common hydrogen bond interactions in diverse phosphoryl transfer active sites. AU - Summerton, Jean C.. AU - Martin, Gregory M.. AU - Evanseck, Jeffrey D.. AU - Chapman, Michael S.. N1 - Publisher Copyright: © 2014 Summerton et al.. PY - 2014/9/19. Y1 - 2014/9/19. N2 - Phosphoryl transfer reactions figure prominently in energy metabolism, signaling, transport and motility. Prior detailed studies of selected systems have highlighted mechanistic features that distinguish different phosphoryl transfer enzymes. Here, a top-down approach is developed for comparing statistically the active site configurations between populations of diverse structures in the Protein Data Bank, and it reveals patterns of hydrogen bonding that transcend enzyme families. Through analysis of large samples of structures, insights are drawn at a level of detail exceeding the experimental precision of an individual structure. In phosphagen kinases, for example, hydrogen bonds with the O3b of the nucleotide ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
UCL Discovery is UCLs open access repository, showcasing and providing access to UCL research outputs from all UCL disciplines.
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The geometries of triafulvene (TF) and its exocyclic Si, Ge, and Sn analogues complexes with F, Cl, Br, and I halogen atoms (TF(X)Y, XC, Si, Ge, and Sn; YF, Cl, Br, and I) were studied. The complexes were optimized at ...
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Arginine kinase (AK), an important member of the phosphokinase family, is involved in temporal and spatial adenosine triphosphate (ATP) buffering systems. AK plays an important role in physiological function and metabolic regulations, in particular tissues with high and fluctuating energy demands. In present study, four AK genes were firstly identified from Yesso scallop (Patinopecten yessoensis) genome, respectively named PyAK1-4. PyAKs have highly conserved structures with a six-exon/five-exon structure, except for PyAK3. PyAK3 contains an unusual two-domain structure and a bridge intron between the two domains, which may originate from gene duplication and subsequent fusion. Phylogenetic analysis showed that all PyAKs belonged to an AK supercluster together with other AK proteins from Mollusca, Platyhelminthes, Arthropoda, and Nematode. A transcriptome database demonstrated that PyAK3 and PyAK4 were the main functional executors with high expression level during larval development and in ...
Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq, Jul 2008 ...
The American cockroach was introduced to the United States from Africa as early as 1625. They belong to the Kingdom: Animalia, Phylum: Arthropoda, Class: Insecta (Insecta: Blattodea: Blattidae), Order: Dictyoptera, and Family: Blattidae. Its common name is American cockroach and scientific name is Periplaneta Americana (Linnaeus). American cockroaches are normally between one and one and a half inches in length. They are oval shaped and reddish-brow with yellow fringes on the area of the body behind the head as adults. They hide in dark crevices during the day, which could include: sewer and drainage systems, cracks and crevices of a house, steam tunnels, large institutional buildings and basements. They emerge at night to hunt for food. Theyll eat pretty much anything. Although it prefers sweets, they have been observed eating paper, boots, hair, bread, fruit, book bindings, fish, peanuts, old rice, putrid sake, the soft part on the inside of animal hides, cloth and dead insects. American ...
In an effort to better understand the life cycle of the blue crab and aid with species restoration, this young naturalist studied the effect of cell area and depth on their growth.
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AL-KO AKS 2004/3004 Side Friction Pads available at Caravan Accessory Shop. Kit comprises of 2 x friction pads and 2 x red plastic caps - to replace side friction pads in an AL-KO AKS 2004 or 3004 stabiliser. Please note: These pads are suitable for both
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1-Vaccenoyl-2-myristoyl-sn-glycerol | C35H66O5 | CID 53478057 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
This research examined effects of hypoxic environments on blue crabs, Callinectes sapidus in an estuarine environment. Hypoxic conditions were treated as a multiple stressor involving low dissolved oxygen (D.O.), increased carbon dioxide (hypercapnia), and low pH concurrently. The objectives were to: 1) identify hypoxiahypercapnia by monitoring D.O. and pH as an indicator of hypercapnia in shallow regions of the York River, 2) measure blue crab abundance, and 3) describe blue crab responses to hypoxiahypercapnia via field work at Taskinas Creek and lab measurements of respiration. Ambient D.O. and pH were positively correlated in the Taskinas Creek and York River sites (r= .73). Crab abundance (CPUE) was not significantly different among D.O. and pH ranges. It was concluded that hemolymph blood lactate concentration was not considered a good in situ biomarker for exposure to hypoxickypercapnic conditions. Oxygen uptake was not significantly different between normoxic and hypoxic conditions but was
Ornithine carbamoyltransferase (OTC) catalyzes the reversible transfer of the carbamoyl group from carbamoyl phosphate (CP) to the Nε atom of L-ornithine (ORN) to produce L-citrulline. There are two types of the enzyme - anabolic (aOTC) and catabolic (cOTC). Anabolic OTCs participate in the urea cycle and L-arginine biosynthesis. Catabolic OTCs are part of the catabolic arginine deiminase pathway found in a number of microorganisms. The reported structure is for the anabolic enzyme from pathogen Vibrio vulnificus. The structure has three monomers in the asymmetric unit which form the physiologically active trimer. One of the three enzyme subunits is entrapped in a complex with its first substrate carbamoyl phosphate and its inhibitor arginine. That complex provides additional structural insights into mechanism of inhibition by arginine. It shows that binding of CP together with arginine causes closure of interdomain cleft and brings catalytic loops K*STRTR, B2-H3 and SMG into the catalytic site ...
AK1 [ENSP00000362271]. Adenylate monophosphate kinase; Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism; Belongs to the adenylate kinase family. AK1 subfamily.. Synonyms: AK1, AK1p, hAK1, AK1-008, AK1-009 .... Linkouts: STRING Pharos UniProt ...
Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates.
Aspartate transaminase (AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or serum glutamic oxaloacetic transaminase (SGOT), is a pyridoxal phosphate (PLP)-dependent transaminase enzyme (EC 2.6.1.1) that was first described by Arthur Karmen and colleagues in 1954. AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate and, as such, is an important enzyme in amino acid metabolism. AST is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells. Serum AST level, serum ALT (alanine transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health. The tests are part of blood panels. Aspartate transaminase catalyzes the interconversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. Aspartate (Asp) + α-ketoglutarate ↔ oxaloacetate + glutamate (Glu) As a prototypical transaminase, AST relies on PLP (Vitamin B6) as a cofactor to transfer the amino group from ...
To enable the resolution of deep phylogenetic divergence in Scolytinae and closely related weevils, several new molecular markers were screened for their phylogenetic potential. The nuclear protein encoding genes, CAD and Arginine Kinase, were particularly promising and will be added to future phylogenetic studies in combination with 28S, COI, and Elongation Factor 1α. The combined analysis of multiple molecular markers and wide taxon sampling is expected to resolve many previously unresolved nodes in scolytine phylogeny, but a completely resolved topology seems dependent on the inclusion of a large number of morphological characters. A well founded phylogeny will provide a powerful framework for testing evolutionary hypotheses on habitat selection and reproductive biology. ...
RNA interference (RNAi), a gene-silencing mechanism that involves providing double-stranded RNA molecules that match a specific target gene sequence, is now widely used in functional genetic studies. The potential application of RNAi-mediated control of agricultural insect pests has rapidly become evident. The production of transgenic plants expressing dsRNA molecules that target essential insect genes could provide a means of specific gene silencing in larvae that feed on these plants, resulting in larval phenotypes that range from loss of appetite to death. In this report, we show that the tomato leafminer (Tuta absoluta), a major threat to commercial tomato production, can be targeted by RNAi. We selected two target genes (Vacuolar ATPase-A and Arginine kinase) based on the RNAi response reported for these genes in other pest species. In view of the lack of an artificial diet for T. absoluta, we used two approaches to deliver dsRNA into tomato leaflets. The first approach was based on the uptake of
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Three energy systems -- commonly referred to as metabolic pathways -- are responsible for the chemical reactions within cells and tissues during exercise and sports. These energy systems include the phosphagen, glycolytic and oxidative pathways.
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Evidence has been presented for the possible transport function of conjugated roach plasma proteins during molting. Extensive changes in these proteins are evident during the premolt stage. The remainder of the instar appears to be devoted to a gradu
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PND-BEACON AKS. Currently serving 8623805 distinct identifiers from 500 beacon sources: -- Titelaufnahmen des B3Kat [4498132]. -- GND-Personen im Verbundkatalog des HBZ (Linked Open Data Bereitstellungen) [4247804]. -- GND to ISNI (via VIAF) [2396394]. -- GND to LC-NAF (via VIAF) [1698410]. -- GND-Personen im SWB-Verbundkatalog (Open Data Bereitstellungen) [1603635]. -- HeBIS-Verbundzentrale [1195183]. -- GND to SUDOC (via VIAF) [929460]. -- GND to BNF (via VIAF) [722345]. -- Bibliothekskatalog Universität Heidelberg [686340]. -- Deutsche Digitale Bibliothek [679557]. -- Korrespondierendes Wikidata-Item (www.wikidata.org) [672081]. -- CERL Thesaurus. Internationaler und multilingualer Thesaurus für Ortsnamen, Drucker, Personen und Körperschaften [639968]. -- Wikipedia-Personenartikel [409075]. -- GND to NKC (via VIAF) [344721]. -- Kalliope Verbundkatalog [316592]. -- The Open Library - Autorenseite (https://openlibrary.org) [280817]. -- INDEX THEOLOGICUS (IxTheo) [236678]. -- GND-Personen im ...
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Herein, we describe the development and application of a set of novel N,N-dimethyl leucine (DiLeu) 4-plex isobaric tandem mass… Expand ...
The hole area o f the TK series polleys (core part) has not been processed, it is delivered in accordance with the size you send as a sample.. • Series (TAL -TEL-TGM-THA-TMO-TSC- TSH- TSİ- TSO-TTH-TTO- TZ) are ready to be you as processed to you as processed according to the original dimensions. Prior to your request, checking the dimensions indicated in the tables in the following pages is important for the absence of errors.. • Serbs (AL -AO - BA - FA - HA - HY- KO - LM-MO - NU- Si) are ready to be delivered to you as processed according to the original dimensions. Prior to your request checking the dimensions indicated in the tables in the following pages is important for the absence of errors. • They are machine-mo hr pulleys. • The material of the pulleys b ductile iron GGG40- ...
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Arginine: | | | Arginine | | | | ||| ... World Heritage Encyclopedia, the aggregation of the largest online encyclopedias available, and the most definitive collection ever assembled.
L arginine plus gnc: exps. 8 and 9. 200 cc. of 1 per cent, glycogen solution containing 2 cc.. l arginine plus zinc the best account of the development of the vascular system in the foetus,
Arginine is one of the compounds responsible for helping blood vessels contract and expand, helping to control blood flow. Most people get all the arginine they need by eating a healthy...
Suurepärane arginiini allikas. See aitab Sind kasvatada lihasmassi ja vabaneda rasvast. Suurendab lämmastikoksiidide taset organiismis, mis omakorda märgatavalt suurendab verevoolu ja hapniku vedamist iga rakule. Mega Arginine stimuleerib immuunsüsteem...
Zinovieva, R.; Piatigorsky, J.; Tomarev, S. I. (1999). "O-Crystallin, arginine kinase and ferritin from the octopus lens". ...
Ghosh G, Adams JA (February 2011). "Phosphorylation mechanism and structure of serine-arginine protein kinases". The FEBS ... Two protein kinases are thought to play a role in the localization of SR proteins in the nucleus. SR protein kinase 1 (SRPK1) ... Eight unphosphorylated repeats of arginine and serine in the RS domain take a helical form with arginine on the outside to ... Methylation of arginine residues in the RRM may also contribute to the export of SR proteins out of the nucleus. SR proteins ...
There has been one case study reported of asthma in a human stemming from an allergy associated with the arginine kinase ... "Arginine Kinase from the Cellar Spider (Holocnemus pluchei): A New Asthma-Causing Allergen". International Archives of Allergy ...
... has the ability to catabolize arginine. Three enzymes are involved in the process; including arginine ... deaminase (ADI), ornithine carbamoyltransferase (OTC), and carbamate kinase (CK). A fourth protein located at the cell membrane ... acts as transporter, allowing the antiporter exchange between arginine and ornithine. A probiotic organism such as ...
Jarilla, B. R.; Uda, K.; Suzuki, T.; Acosta, L. P.; Urabe, M.; Agatsuma, T. (2014). "Characterization of arginine kinase from ...
Arginine kinase (EC 2.7.3.3), which catalyses the transfer of phosphate from ATP to arginine. Taurocyamine kinase (EC 2.7.3.4 ... Strong SJ, Ellington WR (January 1995). "Isolation and sequence analysis of the gene for arginine kinase from the chelicerate ... Lombricine kinase (EC 2.7.3.5), an annelid-specific enzyme that catalyses the transfer of phosphate from ATP to lombricine. ... Creatine kinase (EC 2.7.3.2) (CK), which catalyses the reversible transfer of high energy phosphate from ATP to creatine, ...
In addition to tropomyosin, arginine kinase and hemocyanin seem to have a role in cross-reactivity to dust mites. Exercise can ... Tropomyosin, arginine kinase, myosin light chain and sarcoplasmic calcium-binding protein are widely present across shellfish ... As of a 2016 review, only three (tropomyosin, arginine kinase and sarcoplasmic calcium-binding protein) were available for ... such as arginine kinase, myosin light chain and sarcoplasmic calcium-binding protein. The allergic reaction to fish is to a ...
This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors ... Mylonis I, Giannakouros T (2003). "Protein kinase CK2 phosphorylates and activates the SR protein-specific kinase 1". Biochem. ... "Serine-arginine protein kinase 1 (SRPK1) inhibition as a potential novel targeted therapeutic strategy in prostate cancer". ... "SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors". J. Biol. ...
Serine/arginine protein-specific kinase 2 promotes leukemia cell proliferation by phosphorylating acinus and regulating cyclin ... The OCEL1 protein has been found to interact with two proteins via protein kinase assays; SRPK1, known as SRSF protein kinase I ... and SRPK2, known as SRSF protein kinase II, which are both localized to the nucleus of the cell. SRSF protein kinase I and II ... The Protein Interaction Landscape of the Human CMGC Kinase Group. Cell Reports 3, 1306-1320. Jang, S.-W., Yang, S.-J., Ehlén, A ...
"Identification of an snRNP-associated kinase activity that phosphorylates arginine/serine rich domains typical of splicing ... Cao W, Garcia-Blanco MA (August 1998). "A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient ... Cao W, Garcia-Blanco MA (August 1998). "A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient ... "Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway". The Journal of ...
"Structurally unique yeast and mammalian serine-arginine protein kinases catalyze evolutionarily conserved phosphorylation ... The amino terminus of GBp2 shares a four Arg-Gly-Gly (RGG) repeat motifs and nine serine residues in the context of arginine/ ... The SR domain of GBP2 is a phosphorylation site for SR specific protein kinase SRPK (sky1) which lead a nuclear localization of ...
Arginine +H2N=C(NH2)-NH-(CH2)3- Glutamate → Glutamate-5-semialdehyde (γ-glutamyl kinase) → Arginine ... Glutamic Acid → Glutamate-5-semialdehyde → Proline (γ-glutamyl kinase) Phenylalanine§. Ph-CH2- Phosphoenolpyruvate → 2-keto-3- ... Methylation often occurs to arginine or lysine and involves adding a methyl group to a nitrogen (replacing a hydrogen). The R ... Methylation reduces the ability of these amino acids to form hydrogen bonds so arginine and lysine that are methylated have ...
"The cellular localization of the murine serine/arginine-rich protein kinase CLK2 is regulated by serine 141 autophosphorylation ... Dual specificity protein kinase CLK2 is an enzyme that in humans is encoded by the CLK2 gene. This gene encodes a member of the ... This protein kinase is involved in the regulation of several cellular processes and may serve as a link between cell cycle ... "Entrez Gene: CLK2 CDC-like kinase 2". Human CLK2 genome location and CLK2 gene details page in the UCSC Genome Browser. ...
This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent ... kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs ... Serine/threonine-protein kinase PRP4 homolog is an enzyme that in humans is encoded by the PRPF4B gene. Pre-mRNA splicing ... 2002). "Mammalian PRP4 Kinase Copurifies and Interacts with Components of Both the U5 snRNP and the N-CoR Deacetylase Complexes ...
Dardick C, Schwessinger B, Ronald P (August 2012). "Non-arginine-aspartate (non-RD) kinases are associated with innate immune ... NODs transduce signals in the pathway of NF-κB and MAP kinases via the serine-threonine kinase called RIP2. NODs signal via N- ... Some IRAK and RIP family kinases fall into a small functional class of kinases termed non-RD, many of which do not ... revealed that despite the small number of non-RD kinases in these genomes (9%-29%), 12 of 15 kinases known or predicted to ...
"SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors". J. Biol. ... "SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors". J. Biol. ... Dual specificity protein kinase CLK1 is an enzyme that in humans is encoded by the CLK1 gene. This gene encodes a member of the ... "Entrez Gene: CLK1 CDC-like kinase 1". Colwill K, Feng LL, Yeakley JM, Gish GD, Cáceres JF, Pawson T, Fu XD (Oct 1996). " ...
There was a high level of arginine kinase and certain other enzymes in the adductor muscles which was indicative of the ... conversion of arginine phosphate for energy production. Up to 23% of the ATP used for energy transfer was supplied in this way ... the energy for swimming was supplied aerobically through respiration with little input from anaerobic glycolysis and arginine ...
ATP-binding cassette transporter Arginine phosphorylation by McsB kinase marks proteins for degradation by a Clp protease. The ... Cyclin-dependent kinases (CDKs) are serine-threonine kinases which regulate progression through the eukaryotic cell cycle. CDKs ... Most phosphorylation is carried out by a single superfamily of protein kinases that share a conserved kinase domain. Protein ... Phosphorylation of src tyrosine kinase (pronounced "sarc") by C-terminal Src kinase (Csk) induces a conformational change in ...
SRPK2 is a Serine/arginine rich protein-specific kinase which specifically phosphorylates its substrates at serine residues ... non-kinase and kinase specific), and sumoylation. There is also one leucine-rich nuclear export signal. The entire secondary ... serine/threonine-protein kinase 2) SRPK2 (serine/threonine-protein kinase 2) Function Jenkins D, Beales PL, Wilkie AO (May 2012 ... Produces neural network predictions of kinase specific eukaryotic protein phosphorylation sites Kinase-specific Phosphorylation ...
The expression of genes such as arginine kinase and cavortin is particularly important in regulating the metabolic response of ...
Afadin AFAP-110 Affixin Aginactin AIP1 Aldolase Angiogenin Anilin Annexins Aplyronine Archvillin Arginine kinase Arp2/3 complex ... p21-activated Kinase) Plectin p47PHOX Parvin (actopaxin) Prefoldin p53 PASK (Proline, Alanine rich Ste20 related Kinase) ... Myosin light chain kinase MAL Mip-90 Myosin Light Chain A1 MARKS MIM MAYP Mycalolide (a macroglide drug) Mayven Myelin basic ... actin regulating kinase) Radixin (the R of ERM proteins) Rapsyn Rhizopodin RPL45 RTX toxin (Vibrio cholerae) RVS 167 Sac6 Sla1p ...
Direct interaction with SRPK2 (SRSF Protein Kinase 2), Serine/arginine-rich protein-specific kinase, which phosphorylates ... SRSF protein kinase 2 promotes neural apoptosis by up-regulating cyclin-D1 expression through the suppression of p53/TP53 ... Grueber, Emileigh K. (2013). "Role of ABL Family Kinases in Cancer: from Leukemia to Solid Tumors". Nature Reviews Cancer. 13 ( ... FAM43A shows predicted interaction with the Abelson (ABL) kinase, and ABL members link diverse extracellular stimuli to ...
... has been shown to interact with: CDK9, Cyclin-dependent kinase 7, HTATSF1, PIN1, POLR2A, PRMT1 and Protein arginine ... phosphorylates hSPT5 and RNA polymerase II carboxyl-terminal domain independently of cyclin-dependent kinase-activating kinase ...
This enzyme is also called glycocyamine kinase. This enzyme participates in arginine and proline metabolism. HOBSON GE, REES KR ... Guanidinoacetate kinase belongs to the family of transferases, specifically those that transfer phosphorus-containing groups ( ... In enzymology, a guanidinoacetate kinase (EC 2.7.3.1) is an enzyme that catalyzes the chemical reaction ATP + guanidinoacetate ...
Asparagine, histidine, and arginine residues are involved in catalysis. Human PanK-II isoforms PanK1α, PanK1β, PanK2, and PanK3 ... The regulation of pantothenate kinase is essential to controlling the intracellular CoA concentration. Pantothenate kinase is ... Pantothenate kinase (EC 2.7.1.33, PanK; CoaA) is the first enzyme in the Coenzyme A (CoA) biosynthetic pathway. It ... Consequently, pantothenate kinase is a key regulatory enzyme in the CoA biosynthetic pathway. Three distinct types of PanK has ...
Farago A, Denes G (1967). "Mechanism of arginine biosynthesis in Chlamydomonas reinhardti. II Purification and properties of N- ... In enzymology, an acetylglutamate kinase (EC 2.7.2.8) is an enzyme that catalyzes the chemical reaction: ATP + N-acetyl-L- ... N-acetylglutamate kinase, and N-acetylglutamic 5-phosphotransferase. As of late 2007, 9 structures have been solved for this ... repressible enzymes of arginine synthesis in Escherichia coli". Biochem. Biophys. Res. Commun. 7 (6): 491-6. doi:10.1016/0006- ...
In enzymology, arginine kinase (EC 2.7.3.3) is an enzyme that catalyzes the chemical reaction ATP + L-arginine ⇌ {\displaystyle ... L-arginine N-phosphotransferasel ATP:L-arginine, and ω-N-phosphotransferase. This enzyme participates in arginine and proline ... The systematic name of this enzyme class is ATP:L-arginine Nω-phosphotransferase. Other names in common use include arginine ... the two substrates of this enzyme are ATP and L-arginine, whereas its two products are ADP and Nω-phospho-L-arginine. This ...
"Structural and catalytic role of arginine 88 in Escherichia coli adenylate kinase as evidenced by chemical modification and ... Adenylate kinase, subfamily InterPro: IPR006259 UMP-CMP kinase InterPro: IPR006266 Adenylate kinase, isozyme 1 InterPro: ... adenylate kinase performed dual enzymatic functions. ADK complements nucleoside diphosphate kinase deficiency. Adenylate kinase ... Lu Q, Inouye M (June 1996). "Adenylate kinase complements nucleoside diphosphate kinase deficiency in nucleotide metabolism". ...
This enzyme participates in 4 metabolic pathways: purine metabolism, glutamate metabolism, arginine and proline metabolism, and ... In enzymology, a carbamate kinase (EC 2.7.2.2) is an enzyme that catalyzes the chemical reaction ATP + NH3 + CO2 ⇌ {\ ... Bishop SH, Grisolia S (1966). "Crystalline carbamate kinase". Biochim. Biophys. Acta. 118 (1): 211-5. doi:10.1016/s0926-6593(66 ... I. Preliminary characterization of arginine-specific carbamyl phosphokinase" (PDF). Biochim. Biophys. Acta. 107 (1): 44-53. doi ...
Also, that protein-arginine-phosphatases reverse the effect of protein arginine kinases (PAKs) in living organisms. In B. ... showed that McsB is a protein-arginine-kinase (PAK) and YwlE is a phosphatase-arginine-phosphatase (PAP). Many proteins rely on ... 2012), showed that McsB and YwlE are a protein arginine kinase and phosphatase, rather than a tyrosine kinase and phosphatase ... a protein arginine kinase (PAK). McsB phosphorylates arginine residues in the winged helix-turn-helix domain of CtsR4, ...
NAD(P)+:arginine ADP-ribosyltransferase *Pertussis toxin. *Cholera toxin. *Poly ADP ribose polymerase ...
The encoded protein is a type 1A regulatory subunit of protein kinase A. Inactivating germline mutations of this gene are found ... This revealed a Arg74His (Arginine to Histidine: Guanine (G)-> Adenosine (A) transition in the second codon position of the ...
Arginine methylation[edit]. What was said above of the chemistry of lysine methylation also applies to arginine methylation, ... The mitotic kinase aurora B phosphorylates histone H3 at serine 10, triggering a cascade of changes that mediate mitotic ... Arginine citrullination[edit]. Enzymes called peptidylarginine deiminases (PADs) hydrolyze the imine group of arginines and ... and some protein domains-e.g., Tudor domains-can be specific for methyl arginine instead of methyl lysine. Arginine is known to ...
Jacobia SJ, Korotchkina LG, Patel MS (2001). "Differential effects of two mutations at arginine-234 in the alpha subunit of ... As such, the absolute amounts of site-specific kinases and phosphates expressed in the mitochondria directly affect PDH ... 2001). "Activation and mitochondrial translocation of protein kinase Cdelta are necessary for insulin stimulation of pyruvate ...
cAMP binds to and releases an active form of protein kinase A (PKA). Next, PKA phosphorylates phosphorylase kinase, which, in ... NAD(P)+:arginine ADP-ribosyltransferase *Pertussis toxin. *Cholera toxin. *Poly ADP ribose polymerase ... Glycogen phosphorylase kinase activates glycogen phosphorylase in the same manner mentioned previously. ... The increased calcium availability binds to the calmodulin subunit and activates glycogen phosphorylase kinase. ...
proved that repeated exposure of GHB to MAP kinase affected myelin expression. This is a critical finding since myelin is the ... MAP kinase is imperative for numerous physiological changes including regulation of cell division and differentiation, thus, ... In terms of intracellular signaling, GHB inhibits mitogen activated protein (MAP) kinase action via the GABAB receptor ... Ren, X.; Mody, I. (2003). "Gamma-hydroxybutyrate reduces mitogen-activated protein kinase phosphorylation via GABAB receptor ...
While class I binds via interactions mediated by backbone hydrogen bonds, class II uses a pair of arginine residues to ... "Phosphorylation of glutamyl-prolyl tRNA synthetase by cyclin-dependent kinase 5 dictates transcript-selective translational ... Essential binding site residues (Backbone Brackets and Arginine Tweezers) are colored. N-terminal residues are highlighted in ... This oppositional implementation is manifested in two structural motifs, the Backbone Brackets and Arginine Tweezers, which are ...
positive regulation of protein kinase B signaling. • positive regulation of cell migration. • blood coagulation. • proteolysis ... Its preferred cleavage site (after the arginine in the sequence Ile-Glu/Asp-Gly-Arg, IEGR or IDGR) can easily be engineered ...
Arginine-specificEdit. There are three different types of protein arginine methyltransferases (PRMTs) and three types of ... Branscombe TL, Frankel A, Lee JH, Cook JR, Yang Z, Pestka S, Clarke S (August 2001). "PRMT5 (Janus kinase-binding protein 1) ... The arginine residue and SAM must be correctly oriented within the binding pocket. SAM is secured inside the pocket by a ... McBride AE, Silver PA (July 2001). "State of the arg: protein methylation at arginine comes of age". Cell. 106 (1): 5-8. doi: ...
... s can also synthesize nitric oxide enzymatically, using L-arginine as substrate, as do endothelial cells.[35] ... Excessive eryptosis is observed in red blood cells lacking the cGMP-dependent protein kinase type I or the AMP-activated ... protein kinase AMPK. Inhibitors of eryptosis include erythropoietin, nitric oxide, catecholamines and high concentrations of ...
May also modulate NMDA receptors and protein kinase C.. PO.. Half-life = 5-7 hours.. Neuropathic pain; epilepsy.. Fatigue, ... Comes in free acid and arginine salt forms; practically insoluble in water, soluble in ethanol; degrades upon contact with ...
... protein kinase C (PKC). Both GAD67 and GAD65 are also regulated post-translationally by Pyridoxal 5'-phosphate (PLP); GAD is ... Arginine decarboxylase. *Aromatic L-amino acid decarboxylase. *Glutamate decarboxylase. *Histidine decarboxylase. *Lysine ... GAD67 is phosphorylated at threonine 91 by protein kinase A (PKA), while GAD65 is phosphorylated, and therefore regulated by, ...
These events promote many signaling cascades (such as the MAP kinase pathway) that generate responses like chemotaxis, ... arginine (or ELR for short) immediately before the first cysteine of the CXC motif (ELR-positive), and those without an ELR ... DAG activates another enzyme called protein kinase C (PKC), and IP3 triggers the release of calcium from intracellular stores. ...
Ataxia telangiectasia mutated kinase (ATM kinase)[edit]. ATM kinase, a member of the PI3-like family of serine/threonine ... of which the latter generally involves an arginine residue.[3] [5] ... Src kinases[edit]. The Src-family kinases are examples of proteins that utilize autophosphorylation to sustain their activated ... The probable activation mechanism of src kinase in cancer is as follows: *1. The src kinase is kept in an inactive form through ...
receptor tyrosine kinase binding. • RNA binding. • mRNA binding. Cellular component. • eukaryotic translation initiation factor ... "A region rich in aspartic acid, arginine, tyrosine, and glycine (DRYG) mediates eukaryotic initiation factor 4B (eIF4B) self- ... "Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases". Proc. ...
Arginine vasopressin receptor 1 (V1) on smooth muscle cells Activation of Gq --, ↑PLC activity --, ↑IP3 and DAG --, activation ... The rise in intracellular calcium complexes with calmodulin, which in turn activates myosin light-chain kinase. This enzyme is ... Rho-associated kinase-mediated phosphorylation of MYPT1 at Thr-855 but not Thr-697" (PDF). Biochem. J. 389 (Pt 3): 763-74. doi: ...
SRSF4, SFRS4, SRP75, serine/arginine-rich splicing factor 4, serine and arginine rich splicing factor 4. ... Gui JF؛ Lane WS؛ Fu XD (1994). "A serine kinase regulates intracellular localization of splicing factors in the cell cycle". ... SFRS4‏ (Serine/arginine-rich-splicing factor 4) هوَ بروتين يُشَفر بواسطة جين SFRS4 في الإنسان.[1][2] ... "Entrez Gene: SFRS4 splicing factor, arginine/serine-rich 4". الوسيط ,المسار=. تم تجاهله (مساعدة); الوسيط ,العنوان=. تم تجاهله ( ...
The amino acids that have positively charged side chains at pH 7 are lysine, histidine and arginine.[43] ... Mercer, Donald W (1974). "Separation of tissue and serum creatine kinase isoenzymes by ion-exchange column chromatography". ... 6 arginines for binding to a cation-exchange resin or 6 glutamates for binding to an anion-exchange resin such as DEAE- ... application of ion chromatography is in the rapid anion exchange chromatography technique used to separate creatine kinase (CK ...
Here, A→I editing alters the uncharged amino acid glutamine (Q) to the positively charged arginine (R) in the receptor's ion ... Mauceri D, Cattabeni F, Di Luca M, Gardoni F (May 2004). "Calcium/calmodulin-dependent protein kinase II phosphorylation drives ... element-binding protein through a phosphatidylinositol 3-kinase-dependent stimulation of the mitogen-activated protein kinase ... S818 is phosphorylated by protein kinase C, and is necessary for long-term potentiation (LTP; for GluA1's role in LTP, see ...
Faragó, A. and Dénes, G. (1967). „Mechanism of arginine biosynthesis in Chlamydomonas reinhardti. II. Purification and ... repressible enzymes of arginine synthesis in Escherichia coli". Biochem. Biophys. Res. Commun. 7: 491-496. PMID 13863980.. ...
... phosphorylates hSPT5 and RNA polymerase II carboxyl-terminal domain independently of cyclin-dependent kinase-activating kinase" ... binding and Tat proteins thus belong to the family of arginine-rich motif (ARM) RNA binding proteins.[12] ... "Recruitment of phosphatidylinositol 3-kinase to CD28 inhibits HIV transcription by a Tat-dependent mechanism". J. Immunol. 169 ...
Dipsogenic DI or primary polydipsia results from excessive intake of fluids as opposed to deficiency of arginine vasopressin. ... which couples with protein kinase A, stimulating translocation of the aquaporin 2 channel stored in the cytoplasm of the distal ...
This facilitates the conversion of O2 and L-arginine to NO and L-citrulline. The oxygenase domain of each NOS isoform also ... which leads to hyperpolarization and relaxation Stimulates a cGMP-dependent protein kinase that activates myosin light chain ... nNOS action can be inhibited by NPA (N-propyl-L-arginine). This form of the enzyme is specifically inhibited by 7-nitroindazole ... Nitric oxide synthases produce NO by catalysing a five-electron oxidation of a guanidino nitrogen of L-arginine (L-Arg). ...
positive regulation of protein tyrosine kinase activity. • activation of transmembrane receptor protein tyrosine kinase ... Where C is cysteine, G is glycine, R is arginine, and X represents any amino acid.[14] ... phosphatidylinositol-4,5-bisphosphate 3-kinase activity. Cellular component. • integral component of membrane. • membrane. • ... positive regulation of protein kinase B signaling. • negative regulation of Notch signaling pathway. • regulation of JAK-STAT ...
... kinase and MAP-kinase-activated kinases 1 and 2". European Journal of Biochemistry / FEBS. 217 (2): 715-22. doi:10.1111/j.1432- ... that are phosphorylated by a variety of protein kinases.[12][25] Ser40 is phosphorylated by the cAMP-dependent protein kinase.[ ... mitogen-activated-protein kinase-activating protein kinase) has a preference for Ser40, but also phosphorylates Ser19 about ... 26] Ser19 (and Ser40 to a lesser extent) is phosphorylated by the calcium-calmodulin-dependent protein kinase.[27] MAPKAPK2 ( ...
... predominantly lysine and arginine, which are attached on proteins, lipids and nucleic acids.[22] These glycosylation products ... as well as stimulates intracellular molecules such as Protein Kinase C (PKC), NF-kB and the activation of growth factors TGF-B ...
This in turn leads to increased cAMP-dependent protein kinase or PKA (protein kinase A) activity, ultimately promoting ... In normal conditions, the vascular endothelial nitric oxide synthase produces nitric oxide from L-arginine in the presence of ... The cGMP then activates cGMP-dependent kinase or PKG (protein kinase G). Activated PKG promotes vasorelaxation (via a reduction ... For example, the mitochondrial enzyme pyruvate dehydrogenase kinase (PDK) is pathologically activated in PAH, causing a ...
... serves as an allosteric regulator of pyruvate dehydrogenase kinase (PDK). It regulates through the ratio of acetyl- ... "Regulation of pyruvate dehydrogenase kinase and phosphatase by acetyl-CoA/CoA and NADH/NAD ratios". Biochemical and ...
Kinase. L[cuir in eagar , athraigh foinse]. For substances with an l- or L- prefix such as L-alanine or DL-alanine, please see ... Arginine. *Ascomycin. *Aigéad ascorbach Ascorbic acid (vitamin C). *Asparaigin Asparagine. *Aigéad aspartach Aspartic acid ...
"Interaction of the somatostatin receptor subtype 1 with the human homolog of the Shk1 kinase-binding protein from yeast". J. ... histone-arginine N-methyltransferase activity. • protein-arginine N-methyltransferase activity. • protein-arginine omega-N ... peptidyl-arginine methylation, to symmetrical-dimethyl arginine. • chromatin organization. • histone arginine methylation. ... "Entrez Gene: PRMT5 protein arginine methyltransferase 5".. *^ Stopa N, Krebs JE, Shechter D (June 2015). "The PRMT5 arginine ...
Arginine kinase (EC:2.7.3.3*Search proteins in UniProtKB for this EC number. ... sp,O15992,KARG_ANTJA Arginine kinase OS=Anthopleura japonica OX=67755 PE=1 SV=1 ... PS51510 PHOSPHAGEN_KINASE_C, 2 hits. PS51509 PHOSPHAGEN_KINASE_N, 2 hits. ... PS51510 PHOSPHAGEN_KINASE_C, 2 hits. PS51509 PHOSPHAGEN_KINASE_N, 2 hits. ...
N-Acetylglutamate kinase (NAGK) catalyses the second step in the route of arginine biosynthesis. In many organisms this enzyme ... N-Acetylglutamate kinase (NAGK) catalyses the second step in the route of arginine biosynthesis. In many organisms this enzyme ... We now shed light on arginine inhibition by determining the crystal structures, at 2.75 A and 2.95 A resolution, of arginine- ... Cloning and Expression of the Gene for the Arginine-Inhibited N-Acetyl-L-Glutamate Kinase from Pseudomonas Aeruginosa, ...
Arginine kinase (EC:2.7.3.3*Search proteins in UniProtKB for this EC number. ... sp,Q9GYX1,KARG_PACMR Arginine kinase OS=Pachygrapsus marmoratus OX=135190 PE=2 SV=1 ... PS51510 PHOSPHAGEN_KINASE_C, 1 hit. PS51509 PHOSPHAGEN_KINASE_N, 1 hit. ... PS51510 PHOSPHAGEN_KINASE_C, 1 hit. PS51509 PHOSPHAGEN_KINASE_N, 1 hit. ...
In enzymology, arginine kinase (EC 2.7.3.3) is an enzyme that catalyzes the chemical reaction ATP + L-arginine ⇌ {\displaystyle ... L-arginine N-phosphotransferasel ATP:L-arginine, and ω-N-phosphotransferase. This enzyme participates in arginine and proline ... The systematic name of this enzyme class is ATP:L-arginine Nω-phosphotransferase. Other names in common use include arginine ... the two substrates of this enzyme are ATP and L-arginine, whereas its two products are ADP and Nω-phospho-L-arginine. This ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Arginine kinase (AK), a homologue of creatine kinase, catalyses the reversible transfer of a phosphoryl group between a ... Critical initial real-space refinement in the structure determination of arginine kinase.. Zhou G1, Somasundaram T, Blanc E, ... The family of phosphagen kinases eluded structure determination for over 25 years until an inactive form creatine kinase (CK) ...
... cell division protein kinase 12 , CDC2-related kinase 7 , CDC2-related kinase, arginine/serine-rich , protein kinase for ... Target Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) * Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) ... Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) Antigen Profile Protein Summary human homolog may play a role in regulation ... Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) ELISA Kit ELISA Kit CRKRS Reactivity: Cow Colorimetric Sandwich ELISA 78 pg/ ...
Serine/Arginine-Rich Protein Kinase Blockade Attenuates Choroidal Neovascularization Zhenyu Dong; Kousuke Noda; Atsuhiro Kanda ... Serine/Arginine-Rich Protein Kinase Blockade Attenuates Choroidal Neovascularization You will receive an email whenever this ... Purpose: To investigate the applicability of serine/arginine-rich protein kinase (SRPK)-specific inhibitor, SRPIN340, for ... Serine/Arginine-Rich Protein Kinase Blockade Attenuates Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2013;54(15 ...
An siRNA screen revealed that serine-arginine-rich protein kinases linked increased MNK2 activity to decreased eIF4G ... MNK2 Inhibits eIF4G Activation Through a Pathway Involving Serine-Arginine-Rich Protein Kinase in Skeletal Muscle ... MNK2 Inhibits eIF4G Activation Through a Pathway Involving Serine-Arginine-Rich Protein Kinase in Skeletal Muscle ... MNK2 Inhibits eIF4G Activation Through a Pathway Involving Serine-Arginine-Rich Protein Kinase in Skeletal Muscle ...
"Arginine Kinase" by people in this website by year, and whether "Arginine Kinase" was a major or minor topic of these ... "Arginine Kinase" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Arginine Kinase" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Arginine Kinase". ...
To verify our finding that McsB is a protein arginine kinase, we established an in vitro phosphorylation assay (Fig. 3C) using ... A protein kinase can specifically target arginine residues in a transcription factor to regulate its DNA binding. ... A protein kinase can specifically target arginine residues in a transcription factor to regulate its DNA binding. ... Sequence analysis indicated that the McsB protein arginine kinase exhibits no substantial homology to known Ser, Thr, Tyr, or ...
1999). Arginine kinase evolved twice: evidence that echinoderm arginine kinase originated from creatine kinase. Biochem. J 340 ... An estimation of arginine kinase mRNA abundance indicated that acclimation salinity has no effect on arginine kinase gene ... 1994). Evolution of phosphagen kinase. III. Amino acid sequence of arginine kinase from the shrimp Penaeus japonicus. Zool. Sci ... The major invertebrate phosphagen kinase, arginine kinase, is expressed in the gills of two species of euryhaline crabs, the ...
Serine/arginine (SR) protein-specific kinase (SRPK), a family of cell cycle-regulated protein kinases, phosphorylate SR domain- ... Phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein/extracellular signal-regulated kinase kinase 1 (MEK1) ... Serine/Arginine Protein-Specific Kinase 2 Promotes Leukemia Cell Proliferation by Phosphorylating Acinus and Regulating Cyclin ... SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors. J Biol Chem ...
1. Both the monomer arginine kinase from lobster muscle and the dimer arginine kinase from Holothuria forskali catalyse the ATP ... Evolutionary divergence in the mechanism of action of a monomer and a dimer arginine kinase E O Anosike E O Anosike ... Evolutionary divergence in the mechanism of action of a monomer and a dimer arginine kinase. Biochem J 1 June 1976; 155 (3): ... The Mg2+-nucleotide complex is the substrate for this as it is for the kinase reaction. 2. Analogues of arginine inhibit the ...
Serine-Arginine Protein Kinase 1 Overexpression Is Associated with Tumorigenic Imbalance in Mitogen-Activated Protein Kinase ... Serine-Arginine Protein Kinase 1 Overexpression Is Associated with Tumorigenic Imbalance in Mitogen-Activated Protein Kinase ... Serine-Arginine Protein Kinase 1 Overexpression Is Associated with Tumorigenic Imbalance in Mitogen-Activated Protein Kinase ... Serine-Arginine Protein Kinase 1 Overexpression Is Associated with Tumorigenic Imbalance in Mitogen-Activated Protein Kinase ...
... of arginine kinase. For this reason, arginine kinase spot was selected as a potential molecular marker and subjected to tryptic ... Arginine kinase PMF allowed the differentiation of the six species studied. Four samples of commercial origin obtained in local ... Arginine kinase peptide mass fingerprinting as a proteomic approach for species identification and taxonomic analysis of ...
Inhibition of Hepatitis C Virus Replication by a Specific Inhibitor of Serine-Arginine-Rich Protein Kinase. Yuko Karakama, ... SRPIN340 inhibits kinase activities of SRPK1 and SRPK2.The in vitro kinase assays showed that SRPIN340 (Fig. 2 A) inhibited the ... Purification and characterization of a kinase specific for the serine- and arginine-rich pre-mRNA splicing factors. Proc. Natl ... SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors. J. Biol. ...
Inhibition of Hepatitis C Virus Replication by a Specific Inhibitor of Serine-Arginine-Rich Protein Kinase. Yuko Karakama, ... Inhibition of Hepatitis C Virus Replication by a Specific Inhibitor of Serine-Arginine-Rich Protein Kinase ... Inhibition of Hepatitis C Virus Replication by a Specific Inhibitor of Serine-Arginine-Rich Protein Kinase ... Inhibition of Hepatitis C Virus Replication by a Specific Inhibitor of Serine-Arginine-Rich Protein Kinase ...
Evolutionary divergence in the mechanism of action of a monomer and a dimer arginine kinase. E O Anosike, D C Watts ... Evolutionary divergence in the mechanism of action of a monomer and a dimer arginine kinase ... Evolutionary divergence in the mechanism of action of a monomer and a dimer arginine kinase ... Evolutionary divergence in the mechanism of action of a monomer and a dimer arginine kinase ...
The role of arginines in this acetate kinase was investigated. Five arginines (R91, R175, R241, R285, and R340) in the M. ... this approach has only recently become available for acetate kinase. The enzyme from Methanosarcina thermophila has been cloned ... thermophila enzyme were selected for individual replacement based on their high conservation among sequences of acetate kinase ... with the recombinant wild-type enzyme and variants were consistent with the presence of one or more essential arginine residues ...
... an important member of phosphagen kinase family has been extensively studied in various vertebrates and invertebrates. ... From: Arginine kinase from Haemonchus contortus decreased the proliferation and increased the apoptosis of goat PBMCs in vitro ...
... an important member of phosphagen kinase family has been extensively studied in various vertebrates and invertebrates. ... Arginine kinase (AK), being a highly conserved member of the phosphagen kinase (PKs) family, has been studied extensively with ... Arginine kinase (AK), an important member of phosphagen kinase family has been extensively studied in various vertebrates and ... Many excretory/secretory (ES) products of H. contortus including proteins belong to family phosphagen kinase (arginine kinase) ...
I describe the identification and characterization of Drosophila mutants for a newly-identified serine-arginine protein kinase ... I describe the identification and characterization of Drosophila mutants for a newly-identified serine-arginine protein kinase ...
Carbamate kinase: New structural machinery for making carbamoyl phosphate, the common precursor of pyrimidines and arginine. ... The enzymes carbamoyl phosphate synthetase (CPS) and carbamate kinase (CK) make carbamoyl phosphate in the same way: by ATP- ...
Protein kinase C (PKC) is translocated to a phospholipid bilayer by calcium. Once at the membrane protein kinase C undergoes a ... To study the conformational change in PKC in the absence of a membrane, the interactions between an Arginine-rich peptide (ARP ...
The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinaseMAP3K7/TAK1, which is known to ... This protein interacts and thus activatesTAK1 kinase. It has been shown that the C-terminal portion of this protein is ...
... where the protein arginine kinase McsB (7) is mutated and the amount of cellular arginine phosphorylation is dramatically ... mediated through the interplay between protein arginine kinases and the YwlE protein arginine phosphatase activity, presumably ... 2009) McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR. Science 324:1323-1327. ... Our results suggest that in B. subtilis the combined activity of a protein arginine kinase and phosphatase allows a rapid and ...
Role of protein kinase C in arginine vasopressin-stimulated ERK and p70S6 kinase phosphorylation. / Ghosh, Paramita M; Bedolla ... Role of protein kinase C in arginine vasopressin-stimulated ERK and p70S6 kinase phosphorylation. Journal of Cellular ... Fingerprint Dive into the research topics of Role of protein kinase C in arginine vasopressin-stimulated ERK and p70S6 kinase ... Role of protein kinase C in arginine vasopressin-stimulated ERK and p70S6 kinase phosphorylation. ...
... xanthus arginine kinase, Ark, and demonstrated that it has retained the ability to catalyze the phosphorylation of arginine by ... Recently, putative arginine kinases were identified in several bacterial species, including the social Gram-negative soil ... Arginine kinases catalyze the reversible transfer of a high-energy phosphoryl group from ATP to L-arginine to form ... Arginine kinases catalyze the reversible transfer of a high-energy phosphoryl group from ATP to L-arginine to form ...
The Flagellar Arginine Kinase in Trypanosoma brucei Is Important for Infection in Tsetse Flies. ... We speculate that this flagellar arginine kinase is important for T. brucei infection of tsetse, especially in the context of ... Trypanosoma brucei possesses three different genes for arginine kinase (AK) including one (AK3) that encodes a protein ...
  • An siRNA screen revealed that serine-arginine-rich protein kinases linked increased MNK2 activity to decreased eIF4G phosphorylation. (sciencemag.org)
  • In addition, we found that MNK2 interacted with mTOR and inhibited phosphorylation of the mTOR target, the ribosomal kinase p70S6K (70-kD ribosomal protein S6 kinase), through a mechanism independent of the kinase activity of MNK2. (sciencemag.org)
  • An enzyme that catalyzes the phosphorylation of the guanidine nitrogen of arginine in the presence of ATP and a divalent cation with formation of phosphorylarginine and ADP. (umassmed.edu)
  • The SRPK family of kinases, containing bipartite kinase domains separated by a unique spacer, is mainly localized in the cytoplasm, which is critical for nuclear import of SR proteins in a phosphorylation-dependent manner. (aacrjournals.org)
  • Serine-arginine proteins comprise a family of splice factors that are activated after phosphorylation by SRPKs in the cytoplasm. (aacrjournals.org)
  • Splicing of messenger RNAs is regulated by site-specific binding of members of the serine-arginine-rich (SR) protein family, and SR protein kinases (SRPK) 1 and 2 regulate overall activity of the SR proteins by phosphorylation of their RS domains. (asm.org)
  • The enzymes carbamoyl phosphate synthetase (CPS) and carbamate kinase (CK) make carbamoyl phosphate in the same way: by ATP-phosphorylation of carbamate. (pasteur.fr)
  • Here we report that protein phosphorylation on arginine residues plays a physiologically significant role. (pnas.org)
  • We detected 121 arginine phosphorylation sites in 87 proteins in the Gram-positive model organism Bacillus subtilis in vivo. (pnas.org)
  • Moreover, we provide evidence that protein arginine phosphorylation has a functional role and is involved in the regulation of many critical cellular processes, such as protein degradation, motility, competence, and stringent and stress responses. (pnas.org)
  • Our results suggest that in B. subtilis the combined activity of a protein arginine kinase and phosphatase allows a rapid and reversible regulation of protein activity and that protein arginine phosphorylation can play a physiologically important and regulatory role in bacteria. (pnas.org)
  • These observations suggest that McsB-mediated protein arginine phosphorylation could occur in bacteria, but neither the occurrence of the modification in vivo itself nor a physiological function for it has yet been described. (pnas.org)
  • Here we demonstrate that protein arginine phosphorylation does take place and is functional inside living cells. (pnas.org)
  • Moreover, we demonstrated a physiological role for protein arginine phosphorylation in the case of the Hsp100/Clp protein ClpC, because we observe that the ClpC activity is regulated through a McsB-dependent phosphorylation on two arginine residues. (pnas.org)
  • In addition, we provide evidence that protein arginine phosphorylation plays a significant role for many other regulatory processes within the bacterial cell. (pnas.org)
  • Protein Arginine Phosphorylation Exists in Vivo. (pnas.org)
  • We attempted to identify phosphorylation on protein arginine residues in vivo in a B. subtilis wild-type strain with various proteomic approaches, but failed to detect it. (pnas.org)
  • To study the conformational change in PKC in the absence of a membrane, the interactions between an Arginine-rich peptide (ARP), which underwent cofactor independent phosphorylation, and PKC was investigated. (mcmaster.ca)
  • We previously showed in rat renal glomerular mesangial cells, that arginine vasopressin (AVP)-stimulated cell proliferation was mediated by epidermal growth factor receptor (EGF-R) transactivation, and activation (phosphorylation) of ERK1/2 and p70S6 kinase (Ghosh et al. (elsevier.com)
  • In this paper, we extend these observations and show that different protein kinase C (PKC) isoforms play different roles in mediating AVP-stimulated ERK1/2 and p70S6 kinase phosphorylation and cell proliferation. (elsevier.com)
  • In addition, inhibition of conventional and novel PKC isoforms by chronic (24 h) exposure to phorbol 12-myristate 13-acetate (PMA) inhibited AVP-induced activation of ERK and p70S6 kinase as well as EGF-R phosphorylation. (elsevier.com)
  • Rottlerin, a specific inhibitor of PKCδ, inhibited both ERK and p70S6 kinase phosphorylation and cell proliferation. (elsevier.com)
  • On the other hand, Gö6976, an inhibitor of conventional PKC isoforms, did not affect p70S6 kinase, but stimulated ERK1/2 phosphorylation without affecting cell proliferation. (elsevier.com)
  • In vitro phosphorylation experiments with arginine (R) by lysine (K) substitutions in McsB and its activator McsA unraveled all R residues, which are essentially needed for the arginine kinase reaction. (meta.org)
  • Subsequently, site directed mutagenesis of the MgsR substrate was used to substitute all arginine residues with glutamate (R-E) to mimic arginine phosphorylation and to test their influence on MgsR degradation in vivo. (meta.org)
  • Furthermore, cGMP-dependent protein kinase (G-kinase) activity was reduced in LY-83583-treated cells, as determined by the delay in G-kinase translocation to intermediate filaments and the inhibition of vimentin phosphorylation. (elsevier.com)
  • In vitro reconstitution experiments demonstrate that arginine phosphorylation by the McsB kinase is required and sufficient for the degradation of substrate proteins. (nature.com)
  • This phenomenon appears to be mediated by desensitisation of the V1bR, and may be due to phosphorylation of the receptor by G protein-coupled receptor kinase 5 (GRK5). (canterbury.ac.nz)
  • statement the 1st irreversible SRPK1/2 inhibitor SRPKIN-1, which inhibits phosphorylation of serine/arginine (SR)-wealthy splicing elements proteins and induces a VEGF alternate splicing isoform change, resulting in anti-angiogenesis inside a damp CNV mouse model. (recyclers-info.com)
  • This supports the hypothesis that disordered regions in proteins allow kinases, phosphatases, and phosphorylation-dependent binding proteins to gain access to target sequences to regulate local protein conformation and activity. (mcponline.org)
  • Most well-characterized pattern recognition receptors (PRRs), including Xanthomonas resistance 21, flagellin sensitive 2, and elongation factor-Tu receptor, possess intrinsic protein kinase activity and regulate downstream signaling through phosphorylation events. (frontiersin.org)
  • We also discuss the role of phosphorylation in regulating mitogen-associated protein kinase cascades and transcription factors in plant immune signaling. (frontiersin.org)
  • The importance of creatine kinase localization for the coupling of mitochondrial phosphorylcreatine production to oxidative phosphorylation, J. Biol. (springer.com)
  • The spine is disordered in the inactive kinases and can explain how stabilization of the whole molecule is achieved upon phosphorylation. (pnas.org)
  • All protein kinases catalyze the same chemical reaction in eukaryotic cells: phosphorylation of a recipient protein by transferring the γ-phosphate of ATP to a hydroxyl moiety on a protein substrate. (pnas.org)
  • Arginine modification is a post-translational protein modification in gram-positive bacteria, and protein arginine phosphorylation regulates transcription factors, in addition to tagging rogue proteins for degradation in gram-positive bacteria. (wikipedia.org)
  • It is reversible through the action of kinases (enzymes that adds a phosphate group to a protein via phosphorylation), and this antagonist activity of phosphorylation and dephosphorylation of proteins controls all aspect of prokaryotic and eukaryotic life. (wikipedia.org)
  • 2012), reported in their paper that protein arginine phosphorylation likely plays a critical physiological and regulatory role in bacteria. (wikipedia.org)
  • They showed that protein arginine phosphorylation is involved in the regulation of homeostasis, biofilm formation, motility, competence, stress, and stringent responses by regulating gene expression and protein activity in Bacillus Subtilis. (wikipedia.org)
  • The SR superfamily of splicing factors and regulators is characterized by arginine/serine (RS)-rich domains, which are extensively modified by phosphorylation in cells. (rupress.org)
  • Strikingly, all RS domain-mediated interactions were abolished by SKY1 deletion and were rescuable by yeast or mammalian SR protein-specific kinases, indicating that phosphorylation has a far greater impact on RS domain interactions in vivo than in vitro. (rupress.org)
  • In this report, novel substrate-binding variants of the cAMP-dependent protein kinase (PKA) were used to identify substrate domains required for efficient phosphorylation in vivo . (genetics.org)
  • Evidence suggested that cGK regulated neutrophil spreading, as both VASP phosphorylation and neutrophil spreading were inhibited by Rp-8-pCPT-cGMPS (cGK inhibitor), but not KT5720 (cAMP-dependent protein kinase inhibitor). (jimmunol.org)
  • Phosphorylation of syntaxins, Munc18, and SNAP-23 is catalyzed by kinases such as protein kinase C (PKC) ( 12 , 13 , 14 ). (jimmunol.org)
  • In mast cells, a mechanism of degranulation induced by FcεRI cross-linking involves recruitment and activation of the tyrosine kinases Lyn and Syk, phosphorylation of several tyrosine residues in FcεRI, activation of phospholipase Cγ (PLCγ), and generation of the second messengers diacylglycerol and inositol trisphosphate ( 1 , 15 ). (jimmunol.org)
  • Arginine causes phosphorylation of focal adhesion kinase (FAK), an enzyme whose activation enhances cell migration. (bmj.com)
  • Torres characterized enzymes controlling glycogen metabolism through phosphorylation/dephosphorylation mechanisms involving cyclic nucleotide phosphodiesterases, cyclic AMP and Ca/diacylglycerol stimulated protein kinase, adenylyl cyclase, nitric oxide synthase, G proteins and energy transducing systems - all possible targets of intervention to attack this parasite. (asbmb.org)
  • Moreover, biochemical characterization of McsB revealed that McsB specifically phosphorylates arginine residues in the DNA binding domain of CtsR, thereby impairing its function as a repressor of stress response genes. (sciencemag.org)
  • Ten of the 11 amino acid residues believed to participate in arginine binding are completely conserved among the arthropod sequences analyzed. (biologists.org)
  • RS domains consist of multiple consecutive Arg-Ser/Ser-Arg dipeptide repeats, in which the Ser residues are extensively phosphorylated by several kinases, including SR protein kinases (SRPKs). (asm.org)
  • Recently, a bacterial protein arginine kinase, McsB, was identified and its activity characterized in vitro ( 7 ), and two McsB substrates, which were phosphorylated on arginine residues, were identified in vitro ( 7 , 8 ). (pnas.org)
  • Among all the peptides, cyclic decapeptide C[RW]5 containing alternative arginine (R) and tryptophan (W) residues was found to be the most potent Src kinase inhibitor. (chapman.edu)
  • Quantitative affinity proteomics using a ClpP-trapping mutant show that proteins phosphorylated on arginine residues are selectively targeted to ClpC-ClpP. (nature.com)
  • The surface comparison of different serine-threonine and tyrosine kinases reveals a set of 30 residues whose spatial positions are highly conserved. (pnas.org)
  • The spine is comprised of four hydrophobic residues that we detected in a set of 23 eukaryotic and prokaryotic kinases. (pnas.org)
  • Surface comparison of two active protein kinases (PKA and Cdk2) is capable to detect the most conserved, functionally important residues. (pnas.org)
  • Protein Arginine Phosphatase (PAPs), also known as Phosphoarginine Phosphatase, is an enzyme that catalyzes the dephosphorylation of phosphoarginine residues in proteins. (wikipedia.org)
  • McsB phosphorylates arginine residues in the winged helix-turn-helix domain of CtsR4, preventing it from binding to DNA, allowing for the expression of the repressed gene. (wikipedia.org)
  • Kinases with such residues are termed RD kinases, although most RLKs implicated in microbial detection are non-RD kinases, lacking an arginine preceding the catalytic aspartate. (frontiersin.org)
  • A protein kinase phosphorylates arginine residues in a transcriptional factor during the bacterial heat shock response. (sciencemag.org)
  • This spatial organization is conserved among the entire eukaryotic protein kinase family, and alteration of these residues in a second, unrelated protein kinase also resulted in a stable association with substrates. (genetics.org)
  • Enzymes that catalyze the methylation of arginine residues of proteins to yield N-mono- and N,N-dimethylarginine. (bioportfolio.com)
  • To investigate the applicability of serine/arginine-rich protein kinase (SRPK)-specific inhibitor, SRPIN340, for attenuation of choroidal neovascularization (CNV) formation using a mouse model. (arvojournals.org)
  • Serine/arginine (SR) protein-specific kinase (SRPK), a family of cell cycle-regulated protein kinases, phosphorylate SR domain-containing proteins in nuclear speckles and mediate the pre-mRNA splicing. (aacrjournals.org)
  • Two families of kinases, SRPK and Clk/Sty, have been identified that phosphorylate SR domain-containing splicing factors. (aacrjournals.org)
  • We recently reported the elevation of a kinase [serine-arginine protein kinase (SRPK) 1] in pancreatic carcinoma cells that is critical for the regulation of various mRNA splicing factors ( 1 ). (aacrjournals.org)
  • The SRPK category of kinases regulates pre-mRNA splicing by phosphorylating serine/arginine (SR)-rich splicing factors, signals splicing control in response to extracellular stimuli, and plays a part in tumorigenesis, suggesting these splicing kinases are potential therapeutic targets. (recyclers-info.com)
  • This article is focused on the cAMP-dependent protein kinase (PKA) from the budding yeast, Saccharomyces cerevisiae . (genetics.org)
  • VASP is a prominent substrate for both cGMP-dependent protein kinase (cGK) and cAMP-dependent protein kinase. (jimmunol.org)
  • VASP is phosphorylated by cAMP-dependent protein kinase (cAK) or cGMP-dependent protein kinase (cGK). (jimmunol.org)
  • To determine the physiological role of the cAMP-dependent protein kinase (PKA), a mouse model was developed to increase PKA activity specifically in the pancreatic β-cells. (diabetesjournals.org)
  • An increase in cAMP concentration activates the cAMP-dependent protein kinase (PKA) and guanine-nucleotide exchange protein activated by cAMP (EPAC) ( 10 , 11 ). (diabetesjournals.org)
  • In contrast, a PKCε translocation inhibitor decreased ERK1/2 activation without affecting p70S6 kinase or cell proliferation, while a dominant negative PKCζ (K281W) cDNA delayed p70S6 kinase activation without affecting ERK1/2. (elsevier.com)
  • C[RW]5 was a noncompetitive Src kinase inhibitor, showing approximately fourfold more selectivity towards Src than Abl. (chapman.edu)
  • The effects of guanosine 3,'5'-cyclic monophosphate (cGMP) on the secretory response of activated human neutrophils were investigated using LY- 83583, an inhibitor of soluble guanylate cyclase, and L-arginine, the precursor of nitric oxide formation. (elsevier.com)
  • 2009). Nevertheless, most Meals and Medication Administration (FDA)-authorized VEGFR inhibitors are pan-receptor WAY-100635 maleate salt manufacture tyrosine kinase (RTK) inhibitors, and creating a selective VEGFR inhibitor is a problem. (recyclers-info.com)
  • The authors showed not only that Arginine stimulates migration at physiological contractions, but also that this effect can be substantially reduced by the nitric oxide (NO) synthetase inhibitor, NMMA. (bmj.com)
  • abstract = "Biochemical comparison of arginine kinase allozymes in Drosophila melanogaster. (ncue.edu.tw)
  • The 3-D structure of homodimeric, arginine-insensitive, Escherichia coli NAGK, clarified substrate binding and catalysis but shed no light on arginine inhibition of NAGK. (rcsb.org)
  • The Mg2+-nucleotide complex is the substrate for this as it is for the kinase reaction. (portlandpress.com)
  • This is further decreased to 65% by the addition of L-arginine, indicating that this substrate does bind to the thiolmodified enzyme. (portlandpress.com)
  • Kinetic Analyses of the Substrate Inhibition of Paramecium Arginine Kinase. (nih.gov)
  • Characterization of four arginine kinases in the ciliate Paramecium tetraurelia: Investigation on the substrate inhibition mechanism. (nih.gov)
  • A ) Diagram of known interactions between the protein kinase catalytic core, ATP and a substrate (PKA numbering is used). (pnas.org)
  • Interestingly, one of these PKA variants was altered at a residue that is conserved in all protein kinases, suggesting that it might be possible to generate substrate-binding versions of other enzymes in this family. (genetics.org)
  • These variants of PKA were used here to explore the nature of the protein kinase-substrate interaction. (genetics.org)
  • In enzymology, arginine kinase (EC 2.7.3.3) is an enzyme that catalyzes the chemical reaction ATP + L-arginine ⇌ {\displaystyle \rightleftharpoons } ADP + Nω-phospho-L-arginine Thus, the two substrates of this enzyme are ATP and L-arginine, whereas its two products are ADP and Nω-phospho-L-arginine. (wikipedia.org)
  • The systematic name of this enzyme class is ATP:L-arginine Nω-phosphotransferase. (wikipedia.org)
  • This enzyme participates in arginine and proline metabolism. (wikipedia.org)
  • In many organisms this enzyme is inhibited by the final product of the route, arginine, and thus plays a central regulatory role. (rcsb.org)
  • These signatures are found also in the homologous arginine-inhibited enzyme NAG synthase. (rcsb.org)
  • 2. Analogues of arginine inhibit the exchange reaction of the lobster enzyme but enhance that of the Holothuria enzyme. (portlandpress.com)
  • 3. With the lobster enzyme NO3- has no effect on the exchange reaction alone and inhibit only slightly the apparent enhancement of the exchange reaction produced by the addition of arginine. (portlandpress.com)
  • This is compatible with previous findings for this enzyme that formation of the anion-stabilized dead-end complex, enzyme-arginine-MgADP-NO3-, does not occur to any marked degree. (portlandpress.com)
  • From the effects of arginine and related compounds it would appear that during the normal kinase reaction the partial ATP-ADP exchange reaction is suppressed in the lobster enzyme but enhanced in the Holothuria enzyme. (portlandpress.com)
  • phase II trial is studying how well L-arginine supplementation works with or without enzyme inhibitors in treating erectile function and quality of life of prostate cancer survivors previously treated with radiation therapy. (bioportfolio.com)
  • However, this modified protein has only 1% of the maximum catalytic activity of the wild-type enzyme and 5- and 85-fold higher apparent Km values for ATP and AMP, respectively, than the parent adenylate kinase. (canada.ca)
  • Arg-88, which is a highly conserved residue in all known molecular forms of adenylate kinases (corresponding to Arg-97 in muscle cytosolic enzyme), should be located inside a big cleft of the molecule, close to the phosphate-binding loop. (canada.ca)
  • The pleiotropic effects of creatine (Cr) are based mostly on the functions of the enzyme creatine kinase (CK) and its high-energy product phosphocreatine (PCr). (springer.com)
  • An enzyme that activates arginine with its specific transfer RNA. (bioportfolio.com)
  • and the finding that the biosynthetic enzyme arginine kinase is evolutionarily related to arthropod arginine kinase, suggesting horizontal gene transfer. (asbmb.org)
  • These data suggest that activators of neutrophil degranulation mediate this response through a cGMP- dependent protein kinase mechanism. (elsevier.com)
  • Wyatt, TA , Lincoln, TM & Pryzwansky, KB 1993, ' Regulation of human neutrophil degranulation by LY-83583 and L-arginine: Role of cGMP-dependent protein kinase ', American Journal of Physiology - Cell Physiology , vol. 265, no. 1 34-1, pp. (elsevier.com)
  • We examined the roles of cGMP-dependent protein kinase (PKG) and PI3K in degranulation induced by fMLF and by FcεRI cross-linking. (jimmunol.org)
  • In agreement with this observation, cdc2 kinase, a cdc2/cyclin B complex essential for G 2 -M phase transition, phosphorylates SF2/ASF ( 11 ). (aacrjournals.org)
  • Serine-arginine protein kinases 2 (SRPK2) is a cell cycle-regulated kinase that phosphorylates serine/arginine domain-containing proteins and mediates pre-mRNA splicing with unclear function in neurons. (jneurosci.org)
  • A second, well-characterized target for mTOR signaling is the 70-kDa protein kinase that phosphorylates ribosomal protein S6 (S6K1) ( 2 ). (asm.org)
  • We now shed light on arginine inhibition by determining the crystal structures, at 2.75 A and 2.95 A resolution, of arginine-complexed Thermotoga maritima and arginine-free Pseudomonas aeruginosa NAGKs, respectively. (rcsb.org)
  • Acetylglutamate counters arginine inhibition by promoting active centre closure. (rcsb.org)
  • The hexameric architecture justifies the observed sigmoidal arginine inhibition kinetics with a high Hill coefficient (N approximately 4), and appears essential for arginine inhibition and for NAGK-PII complex formation, since this complex may involve binding of NAGK and PII with their 3-fold axes aligned. (rcsb.org)
  • The NAGK structures allow identification of diagnostic sequence signatures for arginine inhibition. (rcsb.org)
  • The findings on NAGK shed light on the structure, function and arginine inhibition of this synthase, for which a hexameric model is constructed. (rcsb.org)
  • We have also examined the effect of selective inhibition of this kinase on cancer-relevant processes. (aacrjournals.org)
  • These results suggest that central treatment with SIM decreased sympathetic outflow in CHF rabbits via up-regulation of nNOS expression in RVLM, which may be due to the inhibition of 3-hydroxy-3-methylglutaryl-CoA reductase and a decrease in Rho kinase by SIM. (aspetjournals.org)
  • Protein kinases are important mediators of signal transduction in eukaryotic cells, and identifying the substrates of these enzymes is essential for a complete understanding of most signaling networks. (genetics.org)
  • PROTEIN kinases are key mediators of signal transduction in all eukaryotic cells. (genetics.org)
  • Splicing factor arginine/serine-rich 17A (SFRS17A) is an A-kinase anchoring protein that targets protein kinase A to splicing factor compartments. (uky.edu)
  • A bioinformatic search with the consensus RI specifier region identified a novel AKAP, the splicing factor arginine/serine-rich 17A (SFRS17A). (uky.edu)
  • The major invertebrate phosphagen kinase, arginine kinase, is expressed in the gills of two species of euryhaline crabs, the blue crab Callinectes sapidus and the shore crab Carcinus maenas, in which energy-requiring functions include monovalent ion transport, acid-base balance, nitrogen excretion and gas exchange. (biologists.org)
  • 1994 ). Evolution of phosphagen kinase. (biologists.org)
  • Arginine kinase (AK), an important member of phosphagen kinase family has been extensively studied in various vertebrates and invertebrates. (biomedcentral.com)
  • Arginine kinase (AK), a homologue of creatine kinase, catalyses the reversible transfer of a phosphoryl group between a guanidino phosphate and ADP. (nih.gov)
  • The family of phosphagen kinases eluded structure determination for over 25 years until an inactive form creatine kinase (CK) structure was determined [Fritz-Wolf et al. (nih.gov)
  • W. E. Jacobus and A. L. Lehninger, Creatine kinase of rat heart mitochondria. (springer.com)
  • R. F. G. Booth and J. B. Clark, Studies on the microchondrially bound form of rat brain creatine kinase, Biochem. (springer.com)
  • 2008 ). Although Cr effects are likely due to pleiotropic cellular functions, its main role is in the creatine kinase (CK/PCr) system for temporal and spatial energy buffering. (springer.com)
  • Zinc induces unfolding and aggregation of dimeric arginine kinase by trapping reversible unfolding intermediate. (semanticscholar.org)
  • Identification of the CtsR/McsB arginine phospho-switch expands the repertoire of possible protein modifications involved in prokaryotic and eukaryotic transcriptional regulation. (sciencemag.org)
  • McsB shows pronounced homology to phosphagen kinases (PhKs) and has been reported to exhibit tyrosine kinase activity ( 12 , 13 ). (sciencemag.org)
  • A functional McsB kinase is required for the controlled degradation of the global heat-shock regulator CtsR ( 9 ⇓ - 11 ), as well as for the de-localization of competence proteins ( 12 ). (pnas.org)
  • Surprisingly, a protein interaction analysis with MgsR revealed an association with the McsB arginine kinase and an in vivo degradation assay confirmed a strong impact of McsB on MgsR degradation. (meta.org)
  • YwIE was thought to be a protein-tyrosine-phosphatase, and McsB a tyrosine-kinase, however in 2012 Elsholz et al. (wikipedia.org)
  • showed that McsB is a protein-arginine-kinase (PAK) and YwlE is a phosphatase-arginine-phosphatase (PAP). (wikipedia.org)
  • 2005) found that McsB is a tyrosine kinase that needs McsA to become activated. (wikipedia.org)
  • Bacterial histidine kinases (HK) are associates from the GHKL superfamily, which share a distinctive adenosine triphosphate (ATP)-binding Bergerat fold. (welbourneprimary.com)
  • DYRK1A contains a bipartite nuclear localization signal within its kinase domain that is required for nuclear localization, and a C-terminal poly-histidine tract that is required for nuclear speckle localization 25 and phase-separation with RNA polymerase II 24 . (nature.com)
  • Our results demonstrate that SRPKs are host factors essential for HCV replication and that functional inhibitors of these kinases may constitute a new class of antiviral agents against HCV infection. (asm.org)
  • Targeting acetate kinase: inhibitors as potential bacteriostatics. (semanticscholar.org)
  • Evaluate the toxicity of treatment with L-arginine with or without phosphodiesterase-5 inhibitors. (bioportfolio.com)
  • Arginine vasopressin (AVP) is a hypothalamic nonapeptide which regulates the hypothalamic-pituitary-adrenal axis response to stress by stimulating the secretion of adrenocorticotropin (ACTH) from corticotroph cells of the anterior pituitary. (canterbury.ac.nz)
  • Differential activation of arginine-vasopressin receptor subtypes in the amygdaloid modulation of anxiety in the rat by arginine-vasopressin. (bioportfolio.com)
  • The amygdala plays a paramount role in the modulation of anxiety and numerous studies have shown that arginine vasopressin (AVP) elicits anxiogenic effects following either its systemic or septal admi. (bioportfolio.com)
  • Our first phase study has successfully identified tropomyosin and arginine kinase as the major allergens in both species of shellfish. (upsi.edu.my)
  • Therefore, we continued our study to isolate and clone the tropomyosin and arginine kinase from these two species of shellfish, tiger prawn Penaeus monodon and blue swimming crab Portunus trituberculatus . (upsi.edu.my)
  • Tropomyosin and arginine kinase were isolated from the total RNA (Ribonucleic Acid) obtained from both prawn and crab muscles followed by RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction). (upsi.edu.my)
  • Tropomyosin and arginine kinase from the prawn and crab have been isolated and the full-length sequences were obtained. (upsi.edu.my)
  • Current ongoing study focuses on sub-cloning and full-length expression of tropomyosin and arginine kinase in order to produce respective recombinant proteins, and subsequently investigate their physicochemical and allergenic characteristics. (upsi.edu.my)
  • Cloning, Expression, and Characterization of an Acetate Kinase from a High Rate of Biohydrogen Bacterial Strain Ethanoligenens sp. (semanticscholar.org)
  • Characterization of the acetate binding pocket in the Methanosarcina thermophila acetate kinase. (semanticscholar.org)
  • In this thesis, I describe the identification and characterization of Drosophila mutants for a newly-identified serine-arginine protein kinase, which has been termed serine-argining protein kinas at 79D (SRPK79D). (escholarship.org)
  • Identification and Characterization of a Putative Arginine Kinase Homo" by J. Bragg, Andrei Rajkovic et al. (wooster.edu)
  • 1. Both the monomer arginine kinase from lobster muscle and the dimer arginine kinase from Holothuria forskali catalyse the ATP-ADP partial exchange reaction at rates equal to 3 and 0.6% of the normal rate of transphosphorylation respectively. (portlandpress.com)
  • Identification of essential arginines in the acetate kinase from Methanosarcina thermophila. (semanticscholar.org)
  • however, this approach has only recently become available for acetate kinase. (semanticscholar.org)
  • The role of arginines in this acetate kinase was investigated. (semanticscholar.org)
  • Novel pyrophosphate-forming acetate kinase from the protist Entamoeba histolytica. (semanticscholar.org)
  • Acetate kinase and phosphotransacetylase. (semanticscholar.org)
  • Urkinase: structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases. (semanticscholar.org)
  • Clk/Sty was initially cloned as a cyclin-dependent kinase (CDK)-like kinase by PCR ( 4 , 5 ), as well as a dual specificity kinase in an expression screening ( 6 - 8 ). (aacrjournals.org)
  • Alterations in the splice pattern and resulting expression of MAPK kinase are implicated in mediating the antitumoral effects resulting from SRPK1 down-regulation. (aacrjournals.org)
  • We validated an interaction with RNF169, a factor that promotes homology directed repair upon DNA damage, and found that DYRK1A expression and kinase activity are required for maintenance of 53BP1 expression and subsequent recruitment to DNA damage loci. (nature.com)
  • Further, DYRK1A knock out conferred resistance to ionizing radiation in colony formation assays, suggesting that DYRK1A expression decreases cell survival efficiency in response to DNA damage and points to a tumor suppressive role for this kinase. (nature.com)
  • SRPK1 was the first SR protein kinase to be cloned, on the basis of its ability to phosphorylate SR proteins in vitro ( 8 , 9 ), and two other structurally related kinases, SRPK2 and SRPK3, also have been shown to phosphorylate SR proteins ( 16 , 31 ). (asm.org)
  • Evolutionary variation between a monomer and a dimer arginine kinase. (portlandpress.com)
  • Site-directed mutagenesis of Arg-88 to glycine yields a modified form of adenylate kinase (RG88 mutant) closely related structurally to the wild-type protein as indicated by Fourier transform infrared spectroscopy, differential scanning calorimetry, and limited proteolysis. (canada.ca)
  • Seasonal levels of the major American cockroach allergen per a 9 (arginine kinase) in Bangkok and their rele-vance for disease severity. (bvsalud.org)
  • 2010). Once in the nucleus, SRPK1 can synergize with extra SR proteins kinases, like the CLK category of kinases mainly localized in the nucleus, to help expand phosphorylate SR protein to market spliceosome set up (Aubol et al. (recyclers-info.com)
  • Our results indicate that PKCδ plays an important role in AVP-stimulated ERK and p70S6 kinase activation and cell proliferation. (elsevier.com)
  • SR protein-specific kinase 1 (SRPK1) and SRPK2 are regulated by the cell cycle and are specific for SR proteins ( 3 ). (aacrjournals.org)
  • N-Acetylglutamate kinase (NAGK) catalyses the second step in the route of arginine biosynthesis. (rcsb.org)
  • Acute falciparum malaria is associated with low plasma arginine and impaired nitric oxide (NO) production. (bioportfolio.com)
  • This study will test the effect of arginine versus citrulline versus placebo supplementation In three groups of ketosis-prone diabetes (KPD) patients on arginine and nitric oxide productio. (bioportfolio.com)
  • Arginine, a widely available dietary amino acid, is a precursor of nitric oxide which itself stimulates epithelial cell migration. (bmj.com)
  • Belongs to the protein kinase superfamily. (abcam.com)
  • The enzymatic activity of arginine kinase approximately doubles in the ion-transporting gills of C. sapidus, a strong osmoregulator, when the crabs are transferred from high to low salinity, but does not change in C. maenas, a more modest osmoregulator. (biologists.org)
  • Removal of the spacer in SRPK1 has little effect on the kinase activity, but triggers the nuclear translocation of kinases and consequently induces aggregation of splicing factors in the nucleus. (aacrjournals.org)
  • Arginine kinase is a 40 to 42 kDa heat-labile protein that plays an important role in regenerating adenosine triphosphate (ATP) during bursts of cellular activity [5]. (upsi.edu.my)
  • and protein serine/threonine kinase activity. (jax.org)
  • Modulation of protein kinase activity is vitally important, and their malfunction leads to many diseases. (pnas.org)
  • They act by removing a phosphate group from proteins, and their activity counteracts that of protein kinases. (wikipedia.org)
  • Their results suggested that the combined action of protein arginine phosphatase and kinase allows for rapid and reversible regulation of protein activity. (wikipedia.org)
  • A conserved aspartate in the catalytic loop of most kinases is required for catalytic activity. (frontiersin.org)
  • Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. (thermofisher.com)
  • Methods are described for determination of arginine-specific mono-ADP-ribosyltransferase activity of purified proteins and intact cells by monitoring the transfer of ADP-ribose from NAD to a model sub. (bioportfolio.com)
  • Moreover, cell cycle signal induces nuclear translocation of the kinases at the G 2 -M boundary, indicating that SRPKs play a role in cell cycle progression ( 10 ). (aacrjournals.org)
  • Taking advantage of the single nonessential SR protein-specific kinase Sky1p in Saccharomyces cerevisiae , we investigated RS domain interactions in vivo using the two-hybrid assay. (rupress.org)
  • The up-regulation of SRPK1 in multiple cancers and its ability to regulate multiple relevant signaling pathways provide support for developing agents to inhibit this kinase for possible broad application to treat epithelial cancers. (aacrjournals.org)
  • To date, numerous protein kinase structures have been solved in both active and inactive states, which has provided a general understanding of protein kinase functionality and its regulation ( 1 - 7 ). (pnas.org)
  • Equivalent mammalian receptors from the Pelle/IRAK family differ in usually employing a cytosolic tyrosine kinase domain ( Gish and Clark, 2011 ). (frontiersin.org)
  • Arginine supplementation significantly reduces the incidence of NEC, possibly acting via the mechanisms shown in this paper to enhance intestinal healing. (bmj.com)
  • In renal epithelial cells and colon cancer cells, the choice of VEGF splice isoforms is controlled by the splicing factor SRSF1, phosphorylated by serine-arginine protein kinase 1 (SRPK1). (bris.ac.uk)
  • Phenylglyoxal inactivates Escherichia coli adenylate kinase by modifying a single arginine residue (Arg-88). (canada.ca)
  • Serine-arginine-rich (SR) proteins are a family of non-small nuclear ribonucleoprotein particle (non-snRNP) splicing factors that are highly conserved throughout the eukaryotes. (asm.org)
  • Other names in common use include arginine phosphokinase, adenosine 5'-triphosphate: L-arginine phosphotransferase, adenosine 5'-triphosphate-arginine phosphotransferase, ATP:L-arginine N-phosphotransferasel ATP:L-arginine, and ω-N-phosphotransferase. (wikipedia.org)
  • Phosphagen kinases catalyze the reversible dephosphorylation of guanidino phosphagens such as phosphocreatine and phosphoarginine, contributing to the restoration of adenosine triphosphate concentrations in cells experiencing high and variable demands on their reserves of high-energy phosphates. (biologists.org)
  • Effects of arginine and some analogues of the partial adenosine triphosphate-adenosine diphosphate exchange reaction catalysed by arginine kinase. (portlandpress.com)
  • Arginine kinase (AK), an important member of the phosphokinase family, is involved in temporal and spatial adenosine triphosphate (ATP) buffering systems. (news-oceanacidification-icc.org)
  • Amplification and sequencing of arginine kinase cDNA from both species, accomplished by reverse transcription of gill mRNA and the polymerase chain reaction, revealed an open reading frame coding for a 357-amino-acid protein. (biologists.org)
  • Recent reports have described multifaceted interactions between the mitogen-activated protein kinase (MAPK) and AKT signaling networks and the splice regulatory machinery. (aacrjournals.org)
  • Protein kinases represent a large and diverse class of proteins that play critical roles in intracellular signal transmission. (pnas.org)
  • It spans the molecule and plays a coordinating role in activated kinases. (pnas.org)
  • We found that MNK2 (mitogen-activated protein kinase-interacting kinase 2), but not MNK1, inhibited proteins involved in promoting protein synthesis, including eukaryotic translation initiation factor 4G (eIF4G) and mammalian target of rapamycin (mTOR). (sciencemag.org)
  • Several genes related to cell metabolism, including those involved in the metabolisms of cell wall, carbohydrates and antioxidants, light reactions, biotic and abiotic stress responses, as well as genes coding for transcription factors (TFs), protein kinases (PKs) and proteins involved in the abscisic acid (ABA) and ethylene signaling pathways, were differentially regulated by drought stress. (springer.com)