A reagent that is highly selective for the modification of arginyl residues. It is used to selectively inhibit various enzymes and acts as an energy transfer inhibitor in photophosphorylation.
Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.
Amino Acid Sequence
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
An essential amino acid. It is often added to animal feed.
Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)
Phosphotransferases (Carboxyl Group Acceptor)
A class of enzymes that transfers phosphate groups and has a carboxyl group as an acceptor. EC 2.7.2.
Specific molecular sites or proteins on or in cells to which VASOPRESSINS bind or interact in order to modify the function of the cells. Two types of vasopressin receptor exist, the V1 receptor in the vascular smooth muscle and the V2 receptor in the kidneys. The V1 receptor can be subdivided into V1a and V1b (formerly V3) receptors.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Amino Acid Transport Systems, Basic
Cationic Amino Acid Transporter 1
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
RNA, Transfer, Arg
Amino Acid Substitution
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.
Enzymes of a subclass of TRANSFERASES that catalyze the transfer of an amidino group from donor to acceptor. EC 2.1.4.
Deamino Arginine Vasopressin
Sequence Homology, Amino Acid
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Amino Acid Transport Systems
Proteins prepared by recombinant DNA technology.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Rare autosomal recessive disorder of the urea cycle which leads to the accumulation of argininosuccinic acid in body fluids and severe HYPERAMMONEMIA. Clinical features of the neonatal onset of the disorder include poor feeding, vomiting, lethargy, seizures, tachypnea, coma, and death. Later onset results in milder set of clinical features including vomiting, failure to thrive, irritability, behavioral problems, or psychomotor retardation. Mutations in the ARGININOSUCCINATE LYASE gene cause the disorder.
The monoanhydride of carbamic acid with PHOSPHORIC ACID. It is an important intermediate metabolite and is synthesized enzymatically by CARBAMYL-PHOSPHATE SYNTHASE (AMMONIA) and CARBAMOYL-PHOSPHATE SYNTHASE (GLUTAMINE-HYDROLYZING).
Nitric Oxide Synthase
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Antidiuretic hormones released by the NEUROHYPOPHYSIS of all vertebrates (structure varies with species) to regulate water balance and OSMOLARITY. In general, vasopressin is a nonapeptide consisting of a six-amino-acid ring with a cysteine 1 to cysteine 6 disulfide bridge or an octapeptide containing a CYSTINE. All mammals have arginine vasopressin except the pig with a lysine at position 8. Vasopressin, a vasoconstrictor, acts on the KIDNEY COLLECTING DUCTS to increase water reabsorption, increase blood volume and blood pressure.
Drugs used for their effects on the kidneys' regulation of body fluid composition and volume. The most commonly used are the diuretics. Also included are drugs used for their antidiuretic and uricosuric actions, for their effects on the kidneys' clearance of other drugs, and for diagnosis of renal function.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A rare autosomal recessive disorder of the urea cycle. It is caused by a deficiency of the hepatic enzyme ARGINASE. Arginine is elevated in the blood and cerebrospinal fluid, and periodic HYPERAMMONEMIA may occur. Disease onset is usually in infancy or early childhood. Clinical manifestations include seizures, microcephaly, progressive mental impairment, hypotonia, ataxia, spastic diplegia, and quadriparesis. (From Hum Genet 1993 Mar;91(1):1-5; Menkes, Textbook of Child Neurology, 5th ed, p51)
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
An enzyme that activates arginine with its specific transfer RNA. EC 220.127.116.11.
Pituitary Hormones, Posterior
Hormones released from the neurohypophysis (PITUITARY GLAND, POSTERIOR). They include a number of peptides which are formed in the NEURONS in the HYPOTHALAMUS, bound to NEUROPHYSINS, and stored in the nerve terminals in the posterior pituitary. Upon stimulation, these peptides are released into the hypophysial portal vessel blood.
A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).
Protein Structure, Secondary
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
Protein Processing, Post-Translational
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A genus of gram-negative, mostly facultatively anaerobic bacteria in the family MYCOPLASMATACEAE. The cells are bounded by a PLASMA MEMBRANE and lack a true CELL WALL. Its organisms are pathogens found on the MUCOUS MEMBRANES of humans, ANIMALS, and BIRDS.
Amino Acids, Basic
Amino acids with side chains that are positively charged at physiological pH.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Carrier proteins for OXYTOCIN and VASOPRESSIN. They are polypeptides of about 10-kDa, synthesized in the HYPOTHALAMUS. Neurophysin I is associated with oxytocin and neurophysin II is associated with vasopressin in their respective precursors and during transportation down the axons to the neurohypophysis (PITUITARY GLAND, POSTERIOR).
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A toxic diamine formed by putrefaction from the decarboxylation of arginine and ornithine.
Amino Acid Motifs
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Recombinant Fusion Proteins
A 29-amino acid pancreatic peptide derived from proglucagon which is also the precursor of intestinal GLUCAGON-LIKE PEPTIDES. Glucagon is secreted by PANCREATIC ALPHA CELLS and plays an important role in regulation of BLOOD GLUCOSE concentration, ketone metabolism, and several other biochemical and physiological processes. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p1511)
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Dose-Response Relationship, Drug
A non-essential amino acid naturally occurring in the L-form. Glutamic acid is the most common excitatory neurotransmitter in the CENTRAL NERVOUS SYSTEM.
The process of cleaving a chemical compound by the addition of a molecule of water.
Dentin Desensitizing Agents
The functional hereditary units of BACTERIA.
Electrophoresis, Polyacrylamide Gel
A competitive inhibitor of nitric oxide synthetase.
Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor
Enzymes that catalyze the joining of glutamine-derived ammonia and another molecule. The linkage is in the form of a carbon-nitrogen bond. EC 6.3.5.
Amino Acid Oxidoreductases
Gene Expression Regulation, Bacterial
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Escherichia coli Proteins
Proteins obtained from ESCHERICHIA COLI.
Chromatography, High Pressure Liquid
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.
Carbamoyl-Phosphate Synthase (Ammonia)
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Membrane Transport Proteins
Salts of nitrous acid or compounds containing the group NO2-. The inorganic nitrites of the type MNO2 (where M=metal) are all insoluble, except the alkali nitrites. The organic nitrites may be isomeric, but not identical with the corresponding nitro compounds. (Grant & Hackh's Chemical Dictionary, 5th ed)
A disease that is characterized by frequent urination, excretion of large amounts of dilute URINE, and excessive THIRST. Etiologies of diabetes insipidus include deficiency of antidiuretic hormone (also known as ADH or VASOPRESSIN) secreted by the NEUROHYPOPHYSIS, impaired KIDNEY response to ADH, and impaired hypothalamic regulation of thirst.
A strong organic base existing primarily as guanidium ions at physiological pH. It is found in the urine as a normal product of protein metabolism. It is also used in laboratory research as a protein denaturant. (From Martindale, the Extra Pharmacopoeia, 30th ed and Merck Index, 12th ed) It is also used in the treatment of myasthenia and as a fluorescent probe in HPLC.
Pituitary Gland, Posterior
Neural tissue of the pituitary gland, also known as the neurohypophysis. It consists of the distal AXONS of neurons that produce VASOPRESSIN and OXYTOCIN in the SUPRAOPTIC NUCLEUS and the PARAVENTRICULAR NUCLEUS. These axons travel down through the MEDIAN EMINENCE, the hypothalamic infundibulum of the PITUITARY STALK, to the posterior lobe of the pituitary gland.
Amino Acids, Essential
Amino acids that are not synthesized by the human body in amounts sufficient to carry out physiological functions. They are obtained from dietary foodstuffs.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.
A group of simple proteins that yield basic amino acids on hydrolysis and that occur combined with nucleic acid in the sperm of fish. Protamines contain very few kinds of amino acids. Protamine sulfate combines with heparin to form a stable inactive complex; it is used to neutralize the anticoagulant action of heparin in the treatment of heparin overdose. (From Merck Index, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p692)
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Amino Acid Metabolism, Inborn Errors
Disorders affecting amino acid metabolism. The majority of these disorders are inherited and present in the neonatal period with metabolic disturbances (e.g., ACIDOSIS) and neurologic manifestations. They are present at birth, although they may not become symptomatic until later in life.
The withholding of water in a structured experimental situation.
A peptide of about 41 amino acids that stimulates the release of ADRENOCORTICOTROPIC HORMONE. CRH is synthesized by neurons in the PARAVENTRICULAR NUCLEUS of the HYPOTHALAMUS. After being released into the pituitary portal circulation, CRH stimulates the release of ACTH from the PITUITARY GLAND. CRH can also be synthesized in other tissues, such as PLACENTA; ADRENAL MEDULLA; and TESTIS.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
Nitric Oxide Synthase Type II
ADP Ribose Transferases
Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.
Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Arginine methylation and binding of Hrp1p to the efficiency element for mRNA 3'-end formation. (1/10469)Hrp1p is a heterogeneous ribonucleoprotein (hnRNP) from the yeast Saccharomyces cerevisiae that is involved in the cleavage and polyadenylation of the 3'-end of mRNAs and mRNA export. In addition, Hrplp is one of several RNA-binding proteins that are posttranslationally modified by methylation at arginine residues. By using functional recombinant Hrp1p, we have identified RNA sequences with specific high affinity binding sites. These sites correspond to the efficiency element for mRNA 3'-end formation, UAUAUA. To examine the effect of methylation on specific RNA binding, purified recombinant arginine methyltransferase (Hmt1p) was used to methylate Hrp1p. Methylated Hrp1p binds with the same affinity to UAUAUA-containing RNAs as unmethylated Hrpl p indicating that methylation does not affect specific RNA binding. However, RNA itself inhibits the methylation of Hrp1p and this inhibition is enhanced by RNAs that specifically bind Hrpl p. Taken together, these data support a model in which protein methylation occurs prior to protein-RNA binding in the nucleus. (+info)
Gamma interferon stimulates rat alveolar macrophages to kill Pneumocystis carinii by L-arginine- and tumor necrosis factor-dependent mechanisms. (2/10469)Pneumocystis carinii pneumonia remains a serious complication for immunocompromised patients. In the present study, P. carinii organisms interacted with gamma interferon (IFN-gamma)-stimulated alveolar macrophages (AMs) to activate the L-arginine-dependent cytocidal pathway involving reactive nitrogen intermediates (RNI) that were assayed as nitrite (NO2-). Unstimulated cultures of AMs produced negligible quantities of RNI. Addition of P. carinii organisms to IFN-gamma-primed AMs resulted in greatly enhanced production of RNI. NO2- levels increased from 0.8 +/- 0.4 to 11.1 +/- 3.8 microM as early as 6 h after P. carinii organisms were incubated with IFN-gamma-stimulated AMs and to 35.1 +/- 8.9 microM after a 24-h incubation, a near-maximum level. High levels of NO2- were produced by AMs primed with as little as 10 U of IFN-gamma per ml in the presence of P. carinii, and a 20-fold increase in IFN-gamma concentration resulted in only a further 65% increase in NO2- production. RNI-dependent killing of P. carinii was demonstrated by both a 51Cr release assay and a [35S]methionine pulse immunoprecipitation assay. Addition of either monoclonal tumor necrosis factor alpha (TNF-alpha) neutralizing antibody or 200 microM NG-monomethyl-L-arginine (L-NGMMA), a competitive inhibitor of the L-arginine-dependent pathway, significantly decreased NO2- production and reduced P. carinii killing. TNF-alpha alone had no effect on P. carinii viability. These results suggest that (i) the specific interaction of P. carinii organisms with IFN-gamma-primed AMs triggers the production of RNI, (ii) RNI are toxic to P. carinii, and (iii) TNF-alpha likely plays a central role in mediating P. carinii killing by IFN-gamma-stimulated AMs. (+info)
Inhibition of transforming growth factor beta production by nitric oxide-treated chondrocytes: implications for matrix synthesis. (3/10469)OBJECTIVE: Nitric oxide (NO) is generated copiously by articular chondrocytes activated by interleukin-1beta (IL-1beta). If NO production is blocked, much of the IL-1beta inhibition of proteoglycan synthesis is prevented. We tested the hypothesis that this inhibitory effect of NO on proteoglycan synthesis is secondary to changes in chondrocyte transforming growth factor beta (TGFbeta). METHODS: Monolayer, primary cultures of lapine articular chondrocytes and cartilage slices were studied. NO production was determined as nitrite accumulation in the medium. TGFbeta bioactivity in chondrocyte- and cartilage-conditioned medium (CM) was measured with the mink lung epithelial cell bioassay. Proteoglycan synthesis was measured as the incorporation of 35S-sodium sulfate into macromolecules separated from unincorporated label by gel filtration on PD-10 columns. RESULTS: IL-1beta increased active TGFbeta in chondrocyte CM by 12 hours; by 24 hours, significant increases in both active and latent TGFbeta were detectable. NG-monomethyl-L-arginine (L-NMA) potentiated the increase in total TGFbeta without affecting the early TGFbeta activation. IL-1beta stimulated a NO-independent, transient increase in TGFbeta3 at 24 hours; however, TGFbeta1 was not changed. When NO synthesis was inhibited with L-NMA, IL-1beta increased CM concentrations of TGFbeta1 from 24-72 hours of culture. L-arginine (10 mM) reversed the inhibitory effect of L-NMA on NO production and blocked the increases in TGFbeta1. Anti-TGFbeta1 antibody prevented the restoration of proteoglycan synthesis by chondrocytes exposed to IL-1beta + L-NMA, confirming that NO inhibition of TGFbeta1 in IL-1beta-treated chondrocytes effected, in part, the decreased proteoglycan synthesis. Furthermore, the increase in TGFbeta and proteoglycan synthesis seen with L-NMA was reversed by the NO donor S-nitroso-N-acetylpenicillamide. Similar results were seen with cartilage slices in organ culture. The autocrine increase in CM TGFbeta1 levels following prior exposure to TGFbeta1 was also blocked by NO. CONCLUSION: NO can modulate proteoglycan synthesis indirectly by decreasing the production of TGFbeta1 by chondrocytes exposed to IL-1beta. It prevents autocrine-stimulated increases in TGFbeta1, thus potentially diminishing the anabolic effects of this cytokine in chondrocytes. (+info)
Phe161 and Arg166 variants of p-hydroxybenzoate hydroxylase. Implications for NADPH recognition and structural stability. (4/10469)Phe161 and Arg166 of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens belong to a newly discovered sequence motif in flavoprotein hydroxylases with a putative dual function in FAD and NADPH binding . To study their role in more detail, Phe161 and Arg166 were selectively changed by site-directed mutagenesis. F161A and F161G are catalytically competent enzymes having a rather poor affinity for NADPH. The catalytic properties of R166K are similar to those of the native enzyme. R166S and R166E show impaired NADPH binding and R166E has lost the ability to bind FAD. The crystal structure of substrate complexed F161A at 2.2 A is indistinguishable from the native enzyme, except for small changes at the site of mutation. The crystal structure of substrate complexed R166S at 2.0 A revealed that Arg166 is important for providing an intimate contact between the FAD binding domain and a long excursion of the substrate binding domain. It is proposed that this interaction is essential for structural stability and for the recognition of the pyrophosphate moiety of NADPH. (+info)
Possible role for ligand binding of histidine 81 in the second transmembrane domain of the rat prostaglandin F2alpha receptor. (5/10469)For the five principal prostanoids PGD2, PGE2, PGF2alpha, prostacyclin and thromboxane A2 eight receptors have been identified that belong to the family of G-protein-coupled receptors. They display an overall homology of merely 30%. However, single amino acids in the transmembrane domains such as an Arg in the seventh transmembrane domain are highly conserved. This Arg has been identified as part of the ligand binding pocket. It interacts with the carboxyl group of the prostanoid. The aim of the current study was to analyze the potential role in ligand binding of His-81 in the second transmembrane domain of the rat PGF2alpha receptor, which is conserved among all PGF2alpha receptors from different species. Molecular modeling suggested that this residue is located in close proximity to the ligand binding pocket Arg 291 in the 7th transmembrane domain. The His81 (H) was exchanged by site-directed mutagenesis to Gln (Q), Asp (D), Arg (R), Ala (A) and Gly (G). The receptor molecules were N-terminally extended by a Flag epitope for immunological detection. All mutant proteins were expressed at levels between 50% and 80% of the wild type construct. The H81Q and H81D receptor bound PGF2alpha with 2-fold and 25-fold lower affinity, respectively, than the wild type receptor. Membranes of cells expressing the H81R, H81A or H81G mutants did not bind significant amounts of PGF2alpha. Wild type receptor and H81Q showed a shallow pH optimum for PGF2alpha binding around pH 5.5 with almost no reduction of binding at higher pH. In contrast the H81D mutant bound PGF2alpha with a sharp optimum at pH 4.5, a pH at which the Asp side chain is partially undissociated and may serve as a hydrogen bond donor as do His and Gln at higher pH values. The data indicate that the His-81 in the second transmembrane domain of the PGF2alpha receptor in concert with Arg-291 in the seventh transmembrane domain may be involved in ligand binding, most likely not by ionic interaction with the prostaglandin's carboxyl group but rather as a hydrogen bond donor. (+info)
R73A and H144Q mutants of the yeast mitochondrial cyclophilin Cpr3 exhibit a low prolyl isomerase activity in both peptide and protein-folding assays. (6/10469)Previously we reported that the R73A and H144Q variants of the yeast cyclophilin Cpr3 were virtually inactive in a protease-coupled peptide assay, but retained activity as catalysts of a proline-limited protein folding reaction [Scholz, C. et al. (1997) FEBS Lett. 414, 69-73]. A reinvestigation revealed that in fact these two mutations strongly decrease the prolyl isomerase activity of Cpr3 in both the peptide and the protein-folding assay. The high folding activities found previously originated from a contamination of the recombinant Cpr3 proteins with the Escherichia coli protein SlyD, a prolyl isomerase that co-purifies with His-tagged proteins. SlyD is inactive in the peptide assay, but highly active in the protein-folding assay. (+info)
The stimulatory effects of Hofmeister ions on the activities of neuronal nitric-oxide synthase. Apparent substrate inhibition by l-arginine is overcome in the presence of protein-destabilizing agents. (7/10469)A variety of monovalent anions and cations were effective in stimulating both calcium ion/calmodulin (Ca2+/CaM)-independent NADPH-cytochrome c reductase activity of, and Ca2+/CaM-dependent nitric oxide (NO.) synthesis by, neuronal nitric oxide synthase (nNOS). The efficacy of the ions in stimulating both activities could be correlated, in general, with their efficacy in precipitating or stabilizing certain proteins, an order referred to as the Hofmeister ion series. In the hemoglobin capture assay, used for measurement of NO. production, apparent substrate inhibition by L-arginine was almost completely reversed by the addition of sodium perchlorate (NaClO4), one of the more effective protein-destabilizing agents tested. Examination of this phenomenon by the assay of L-arginine conversion to L-citrulline revealed that the stimulatory effect of NaClO4 on the reaction was observed only in the presence of oxyhemoglobin or superoxide anion (generated by xanthine and xanthine oxidase), both scavengers of NO. Spectrophotometric examination of nNOS revealed that the addition of NaClO4 and a superoxide-generating system, but neither alone, prevented the increase of heme absorption at 436 nm, which has been attributed to the nitrosyl complex. The data are consistent with the release of autoinhibitory NO. coordinated to the prosthetic group of nNOS, which, in conjunction with an NO. scavenger, causes stimulation of the reaction. (+info)
Cystic fibrosis-associated mutations at arginine 347 alter the pore architecture of CFTR. Evidence for disruption of a salt bridge. (8/10469)Arginine 347 in the sixth transmembrane domain of cystic fibrosis transmembrane conductance regulator (CFTR) is a site of four cystic fibrosis-associated mutations. To better understand the function of Arg-347 and to learn how mutations at this site disrupt channel activity, we mutated Arg-347 to Asp, Cys, Glu, His, Leu, or Lys and examined single-channel function. Every Arg-347 mutation examined, except R347K, had a destabilizing effect on the pore, causing the channel to flutter between two conductance states. Chloride flow through the larger conductance state was similar to that of wild-type CFTR, suggesting that the residue at position 347 does not interact directly with permeating anions. We hypothesized that Arg-347 stabilizes the channel through an electrostatic interaction with an anionic residue in another transmembrane domain. To test this, we mutated anionic residues (Asp-924, Asp-993, and Glu-1104) to Arg in the context of either R347E or R347D mutations. Interestingly, the D924R mutation complemented R347D, yielding a channel that behaved like wild-type CFTR. These data suggest that Arg-347 plays an important structural role in CFTR, at least in part by forming a salt bridge with Asp-924; cystic fibrosis-associated mutations disrupt this interaction. (+info)
Effects of acute and chronic stress on the L-arginine nitric oxide pathway in black and white South Africans: The sympathetic...
TY - JOUR. T1 - Effects of acute and chronic stress on the L-arginine nitric oxide pathway in black and white South Africans. T2 - The sympathetic activity and ambulatory blood pressure in Africans study. AU - Reimann, Manja. AU - Hamer, Mark. AU - Malan, Nicolaas T.. AU - Schlaich, Markus P.. AU - Lambert, Gavin W.. AU - Ziemssen, Tjalf. AU - Boeger, Rainer H.. AU - Malan, Leoné. PY - 2013. Y1 - 2013. N2 - Objective: This study investigated the impact of stress on effectors of the L-arginine/nitric oxide (NO) system including the endogenous inhibitor asymmetric dimethylarginine (ADMA). Methods: Black (n = 168) and white (n = 206) South African teachers were exposed to a mental and a physical stressor for 1 minute, respectively. Serum samples for determination of L-arginine, NO metabolites, ADMA, and symmetric dimethylarginine (SDMA) were obtained at rest and during stress exposure. Perception of task stressfulness was assessed on a 7-point Likert scale, and psychological distress was estimated ...
Endogenous nitric oxide synthase inhibitors, arterial hemodynamics, and subclinical vascular disease: The PREVENCION study<...
TY - JOUR. T1 - Endogenous nitric oxide synthase inhibitors, arterial hemodynamics, and subclinical vascular disease. T2 - The PREVENCION study. AU - Chirinos, Julio A.. AU - David, Robert. AU - Bralley, J. Alexander. AU - Zea-Díaz, Humberto. AU - Muñoz-Atahualpa, Edgar. AU - Corrales-Medina, Fernando. AU - Cuba-Bustinza, Carolina. AU - Chirinos-Pacheco, Julio. AU - Medina-Lezama, Josefina. PY - 2008/12. Y1 - 2008/12. N2 - Endogenous NO synthase inhibitors (end-NOSIs) have been associated with cardiovascular risk factors and atherosclerosis. In addition, end-NOSIs may directly cause hypertension through hemodynamic effects. We aimed to examine the association between end-NOSI asymmetrical dimethylarginine (ADMA) and N-guanidino-monomethyl-arginine (NMMA), subclinical atherosclerosis, and arterial hemodynamics. We studied 922 adults participating in a population-based study (PREVENCION Study) and examined the correlation between end-NOSI/L-arginine and arterial hemodynamics, carotid-femoral ...
PLOS ONE: Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction
Background Fetal Growth Restriction is often associated with a feto-placental vascular dysfunction conceivably involving endothelial cells. Our study aimed to verify this pathogenic role for feto-placental endothelial cells and, coincidentally, demonstrate any abnormality in the nitric oxide system. Methods Prenatal assessment of feto-placental vascular function was combined with measurement of nitric oxide (in the form of S-nitrosohemoglobin) and its nitrite byproduct, and of the endogenous nitric oxide synthase inhibitor asymmetric dimethylarginine. Umbilical vein endothelial cells were also harvested to determine their gene profile. The study comprised term pregnancies with normal (n = 40) or small-for-gestational-age (n = 20) newborns, small-for-gestational-age preterm pregnancies (n = 15), and bi-chorial, bi-amniotic twin pregnancies with discordant fetal growth (n = 12). Results Umbilical blood nitrite (p|0.001) and S-nitrosohemoglobin (p = 0.02) rose with fetal growth restriction while
Metabolism of asymmetric dimethylarginines is regulated in the lung developmentally and with pulmonary hypertension induced by...
BACKGROUND Nitric oxide (NO) plays an important part in lowering pulmonary vascular resistance after birth, and in persistent pulmonary hypertension of the newborn (PPHN), NO-mediated dilation is dysfunctional. The endogenous NO synthase inhibitor asymmetric dimethylarginine (ADMA) circulates in plasma, and its concentrations are elevated in certain cardiovascular diseases, including pulmonary hypertension. ADMA is metabolized by the enzyme dimethylarginine dimethylaminohydrolase (DDAH), the activity of which regulates ADMA concentrations and provides a mechanism for modulating NO synthase in vivo. We investigated the changes in expression and activity of the 2 isoforms of DDAH in lungs from newborn piglets both during normal development and in PPHN. METHODS AND RESULTS Using Western blotting, we showed that DDAHI expression did not change in the normal developing lung; however, DDAHII increased after birth and reached a peak at 1 day. This was reflected in an increase in total DDAH activity according
BLOG) - Communications: No effect of short-term arginine supplementation on nitric oxide production, metabolism and performance...
Arginine supplementation has been shown to alleviate endothelial dysfunction and improve exercise performance through increasing nitric oxide production in patients with cardiopulmonary diseases. In addition, arginine supplementation could decrease accumulations of lactate and ammonia, metabolites involved in development of muscular fatigue. The aim of this study was to investigate the effect of short-term arginine supplementation on performance in intermittent anaerobic exercise and the underlying mechanism in well-trained male athletes. Ten elite male college judo athletes participated with a randomized crossover, placebo-controlled design. The subjects consumed 6 g/day arginine (ARG trial) or placebo (CON trial) for 3 days then performed an intermittent anaerobic exercise test on a cycle ergometer. Blood samples were collected before supplementation, before and during exercise and 0, 3, 6, 10, 30 and 60 min after exercise. ARG trial had significantly higher arginine concentrations than CON ...
Dietary L-arginine supplementation reduces Methotrexate-induced intestinal mucosal injury in rat | BMC Gastroenterology | Full...
Arginine (ARG) and nitric oxide maintain the mucosal integrity of the intestine in various intestinal disorders. In the present study, we evaluated the effects of oral ARG supplementation on intestinal structural changes, enterocyte proliferation and apoptosis following methotrexate (MTX)-induced intestinal damage in a rat. Male rats were divided into four experimental groups: Control rats, CONTR-ARG rats, were treated with oral ARG given in drinking water 72 hours before and 72 hours following vehicle injection, MTX rats were treated with a single dose of methotrexate, and MTX-ARG rats were treated with oral ARG following injection of MTX. Intestinal mucosal damage, mucosal structural changes, enterocyte proliferation and enterocyte apoptosis were determined 72 hours following MTX injection. RT-PCR was used to determine bax and bcl-2 mRNA expression. MTX-ARG rats demonstrated greater jejunal and ileal bowel weight, greater ileal mucosal weight, greater ileal mucosal DNA and protein levels, greater
The association of dimethylarginine dimethylaminohydrolase 1 gene polymorphism with type 2 diabetes: a cohort study |...
In this study, SNP rs1241321 in DDAH1 was found to be associated with a higher risk of type 2 diabetes independently of the plasma ADMA level. In addition, individuals with an AA genotype at rs1241321 appeared to be more insulin-sensitive when compared with AG/GG individuals. Over a median follow-up period of 28.2 months, AA genotype at rs1241321 was associated with better long-term clinical outcome in diabetic subgroup. In contrast, some SNPs of DDAH1, especially the rs1498373, might influence the plasma level of ADMA. However, with the exception of rs1241321, none of these SNPs or the plasma ADMA level was associated with type 2 diabetes, suggesting that the interaction of DDAH1 variants with type 2 diabetes may not be directly related to its enzymatic activity, i.e. not just simply mediated by the plasma ADMA level. We also identified a common haplotype H5 (GGCAGC) that was associated with reduced risk of type 2 diabetes.. It is well recognized that type 2 diabetes and its metabolic ...
Role of l-arginine in nitric oxide production in health and hypertension<...
TY - JOUR. T1 - Role of l-arginine in nitric oxide production in health and hypertension. AU - Rajapakse, Niwanthi W.. AU - Mattson, David L.. PY - 2009/3. Y1 - 2009/3. N2 - l-Arginine is the substrate for vascular nitric oxide (NO) formation. Under normal physiological conditions, intracellular l-arginine levels far exceed the Km of NO synthase for l-arginine. However, endogenous NO formation is dependent on extracellular l-arginine concentrations, giving rise to the concept of the l-arginine paradox. Nitric oxide production in epithelial and endothelial cells is closely coupled to cellular l-arginine uptake, indicating that l-arginine transport mechanisms play a major role in the regulation of NO-dependent function. Consistent with the data in endothelial and epithelial cells are functional data indicating that exogenous l-arginine can increase renal vascular and tubular NO bioavailability and thereby influence kidney perfusion, function and arterial pressure. The integrated effect of ...
Structural behaviour and gene delivery in complexes formed between DNA and arginine-containing peptide amphiphiles - Soft...
We describe in depth the structure of complexes formed between DNA and two classes of arginine-containing peptide amphiphiles, namely, the lipopeptide PRW-C16 (P = proline, R = arginine, W = tryptophan, C16 = C16 : 0 alkyl chain) and the bolaamphiphile RFL4FR (R = arginine, F = phenylalanine, L = leucine). A Biological soft matter
Figure 1 | Serum Symmetric Dimethylarginine as an Early Marker of Excretory Dysfunction in Canine Leishmaniosis (L. infantum)...
Figure 1: Serum Symmetric Dimethylarginine as an Early Marker of Excretory Dysfunction in Canine Leishmaniosis|i| (L. infantum)|/i| Induced Nephropathy
Abstract 1196: Endogenous Nitric Oxide Synthase Inhibitors, Arterial Hemodynamics And Subclinical Vascular Disease: A...
Background: Asymmetric dimethylarginine (ADMA) and NG-monomethyl-L-arginine (NMMA), endogenous nitric oxide-synthase inhibitors (End-NOSI), have been associated with cardiovascular risk factors (CVRF) and atherosclerosis. In addition, acute administration of NOSI cause hypertension through hemodynamic effects, but whether circulating End-NOSI are associated with these abnormalities is unknown.. Methods: We studied 922 adults in a population-based study (PREVENCION study). We examined the correlation between End-NOSI/L-arginine and CVRF, as well as the relationship between these biomarkers and arterial hemodynamics, carotid-femoral pulse wave velocity (cf-PWV) and carotid intima-media thickness (c-IMT). We used general linear modeling for statistical analyses. Estimates (β) are standardized.. Results: In multivariate analyses, LDL-cholesterol and C-reactive protein (CRP) were independent predictors of ADMA, whereas LDL-cholesterol and gender independently predicted NMMA. Age, gender, ...
Effects of Growth Hormone on the Nitric Oxide Pathway - Full Text View - ClinicalTrials.gov
Nitric oxide (NO) is a potent endogenous vasodilator and has shown to inhibit key processes of atherosclerosis like monocyte adhesion, platelet aggregation, and vascular smooth muscle cell proliferation. Impaired endothelial NO production is a main feature of endothelial dysfunction, which by itself is an early step in the course of atherosclerotic vascular disease.. Recent studies could confirm this close association between parameters of the NO pathway and cardiovascular disease and could further enhance the knowledge on the pathophysiological mechanisms. There is a significant relationship between insulin resistance and the endogenous NO synthase inhibitor asymmetric dimethylarginine (ADMA). Moreover, evidence could be provided that plasma levels of ADMA are a strong and independent predictor of mortality and cardiovascular outcome in haemodialysis patients.. Patients with growth hormone deficiency are characterized by a 1.9 fold higher risk of death from cardiovascular disease. Again, there ...
Asymmetric Dimethylarginine, L-Arginine, and Endothelial Dysfunction in Essential Hypertension | JACC: Journal of the American...
The main finding in this study is that in essential hypertensives the endogenous inhibitor of e-NOS ADMA is inversely related to endothelial function as measured by the peak hemodynamic response to ACh. Such relationship occurs in a range of ADMA values within the boundaries of the normal range. A companion, unexpected, finding in this study is that circulating L-arginine is directly related to plasma ADMA and, like plasma ADMA, it is inversely related to endothelial function.. The importance of ADMA as an endogenous inhibitor of e-NOS is now well established (26-28). Elegant studies in healthy volunteers convincingly demonstrated that intravenous ADMA infusion at a dose resulting in pathophysiological concentrations augments peripheral and renovascular resistance and arterial pressure (22). High plasma ADMA concentration was observed in the presence of traditional or emerging cardiovascular risk factors (e.g., hyperhomocysteinemia) (18-21,29), inducing endothelial dysfunction in some of these ...
Publications - Professor Anthony Gordon
AB - BackgroundDimethylarginine dimethylaminohydrolase 2 (DDAH2) regulates the synthesis of nitric oxide (NO) through the metabolism of the endogenous inhibitor of nitric oxide synthase, asymmetric dimethylarginine (ADMA). Pilot studies have associated the rs805305 SNP of DDAH2 with ADMA concentrations in sepsis. This study explored the impact of the rs805305 polymorphism on DDAH activity and outcome in septic shock.MethodsWe undertook a secondary analysis of data and samples collected during the Vasopressin versus noradrenaline as initial therapy in septic shock (VANISH) trial. Plasma and DNA samples isolated from 286 patients recruited into the VANISH trial were analysed. Concentrations of L-Arginine and the methylarginines ADMA and symmetric dimethylarginine (SDMA) were determined from plasma samples. Whole blood and buffy-coat samples were genotyped for polymorphisms of DDAH2. Clinical data collected during the study were used to explore the relationship between circulating methylarginines, ...
Recovery of endothelial function in severe falciparum malaria correlates with recovery of plasma arginine concentrations and...
Depletion of Arginine by Recombinant Arginine Deiminase Induces nNOS-Activated Neurotoxicity in Neuroblastoma Cells
The abnormal regulation of inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase (nNOS) is associated with neurodegenerative disorders. Recombinant arginine deiminase (rADI) is a selective NO modulator of iNOS and eNOS in endothelial cells, and it also exhibits neuroprotective activity in an iNOS-induced neuron-microglia coculture system. However, the effect of rADI on nNOS remains unknown. Addressing this issue is important for evaluating the potential application of rADI in neurodegenerative diseases. SH-SY5Y cells were treated with |i|N|/i|-methyl-D-aspartic acid (NMDA) to activate nNOS. NMDA increased NO production by 39.7 ± 3.9% via nNOS under arginine-containing conditions, but there was no significant increase in both arginine-free and rADI pretreated arginine-containing (citrulline) buffer. Subsequently, neither NMDA nor rADI alone caused cytotoxicity, whereas cotreatment with NMDA and rADI resulted in dissipation of the cell mitochondrial membrane potential and
ADMA(Asymmetrical Dimethylarginine) ELISA Kit - ADMA(Asymmetrical Dimethylarginine) ELISA Kit Exporter, Importer, Manufacturer,...
SR GROUP - Exporter, Importer, Manufacturer, Distributor, Supplier, Trading Company of ADMA(Asymmetrical Dimethylarginine) ELISA Kit based in Delhi, India
Hyperglycemia, asymmetric dimethylarginine, and patient survival: Dysregulation of complex networks and the metabolic basis of...
TY - JOUR. T1 - Hyperglycemia, asymmetric dimethylarginine, and patient survival. T2 - Dysregulation of complex networks and the metabolic basis of disease. AU - Castillo, Leticia. AU - Al-Khadra, Eman. PY - 2005/3/1. Y1 - 2005/3/1. KW - Amino acid pool. KW - Asymmetric dimethylarginine. KW - Nutrients. KW - Peroxisome proliferator-activated receptor-γ. UR - http://www.scopus.com/inward/record.url?scp=14944354288&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=14944354288&partnerID=8YFLogxK. U2 - 10.1097/01.CCM.0000155775.30228.33. DO - 10.1097/01.CCM.0000155775.30228.33. M3 - Editorial. C2 - 15753766. AN - SCOPUS:14944354288. VL - 33. SP - 674. EP - 676. JO - Critical Care Medicine. JF - Critical Care Medicine. SN - 0090-3493. IS - 3. ER - ...
Buy L-arginine Plus® - Get the #1 L-arginine Supplement on the Market | Discover the Difference the #1 L-arginine Supplement...
L-arginine Plus® stands out as the premier l-arginine formula because it is so much more than what you get with most l-arginine products. This formula is recommended more than any other product on the market because it works faster and works more effectively. Dont settle for simple l-arginine when you can get L-arginine Plus®. Heres what comes with every serving of L-arginine Plus®:. 5,110mg L-arginine - L-arginine is the driving force behind L-arginine Plus®. WIth the best l-arginine available, L-arginine Plus® works quickly to help promote nitric oxide production. Take notice because you get more l-arginine in L-arginine Plus® than in any other supplement. 1,010mg L-citrulline - L-citrulline paired with l-arginine ensures long-lasting nitric oxide production for better support for your heart. L-citrulline works over time to enable nitric oxide production that lasts and lasts so you see better results. Astragin™ - Astragin™ is a patented ingredient that sets L-arginine Plus® apart ...
Most recent papers with the keyword Peadiatrics | Read by QxMD
BACKGROUND: Asymmetric dimethylarginine (ADMA) has been suggested as a possible marker of endothelial dysfunction, and interest in its use in clinical practice is increasing. However, the potential role of symmetric dimethylarginine (SDMA) as an endogenous marker of renal function, has been less widely investigated. The aims of the present study were therefore to determine reference values for dimethylarginines in plasma after method validation, and to ascertain ADMA plasma concentrations in patients with disorders characterized by endothelial dysfunction; a further end-point was to investigate the relationship between SDMA plasma concentrations and estimated GFR (eGFR) as well as plasmatic creatinine in patients with chronic kidney disease (CKD ...
The L-arginine-nitric oxide pathway. - PubMed - NCBI
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
euramin CLASSIC | euramin Shop
During an increase in work-related stress or physical activities, the power stations in our cells, the mitochondria, go into increased action and transform fat into energy.. The amino acid carnitine, the so called carrier of long-chained fat molecules, plays a very important role in transporting the right fats to the right places as it allows fats to be more easily assimilated into energy.. Arginine on the other hand is known for its circulatory functions and is therefore important for endurance, vigour and concentration.. euramin CLASSIC is a reliable, approved combination of the amino acids arginine, glutamine, carnitine and methionine, as well as other nutrients, which support the body during times of physical and mental stress, creating a foundation for well-being and vitality.. ...
Effect of arginine on the lipid and lipoprotein content of animal blood
Abstract: Arginine decreased cholesterol and triglyceride content in blood sera of intact rats and inhibited the development of hyperlipidemia provoked by Triton WR-1339 injection. In rabbits pretreated with cholesterol arginine diminished the content of blood serum cholesterol and triglycerides. Introduction of arginine to intact and hyperlipidemic guinea pigs decreased the VLDL and increased the HDL level. Under influence of arginine electrophoretic zone of HDL apo A-1 was more pronounced and apo E zone became less distinct in hyperlipidemic guinea pigs ...
Dabrafenib - BET-bromodomain inhibition research
Bile duct ligation (BDL)-treated rats exhibit cholestasis, increased systemic oxidative tension, and liver fibrosis, which ultimately lead to liver cirrhosis. levels by postnatal day time 2 (~0.66 M) . In children, plasma ADMA levels are higher than those in adults, and gradually Dabrafenib diminish from birth until around 25 years of age, having a mean decrease of 15 nM per year [6C8]. A healthy adult generates 300 mol (~60 mg) of ADMA per day . Bode-Bogers found a significant increase in plasma levels of ADMA in subjects more than 70 years . By inhibiting NO bioavailability, ADMA causes endothelial dysfunction, vasoconstriction, blood pressure elevation and atherosclerosis [11C16]. Increasing evidence reveals that elevated ADMA is associated with many diseases such as peripheral arterial disease, coronary artery disease, preeclampsia, hypertension, heart stroke, heart failing, chronic kidney disease, portal hypertension in cirrhosis, diabetes mellitus, and insulin level of resistance ...
L-Citrulline Malate: A compound consisting of Citrulline, a nonessential amino acid. It plays a vital role in many important biological processes including regulating nitric oxide production, helping to eliminate the burn associated with lactic acid build up, reducing the negative effects of ammonia and bacterial endo toxins on performance, increasing the rate of muscle ATP (Andenosine Triphosphate, the major energy source within the cell) production during exercise and greater phosphocreatine recovery after exercise. In fact, L-Citrulline Malate seems to increase plasma Arginine levels better than taking the amino acid Arginine itself! If the enhanced pumps and improved nutrient delivery properties are the reason you are using Arginine based NO products, you need to pay close attention to L-Citrulline Malate as this may result in muscles that can keep on going and going…completely smashing the aerobic threshold ...
FRI0100 The Role of Insulin Resistance and Inflammation on Symmetric Dimethylarginine in Rheumatoid Arthritis | Annals of the...
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FertileMax | Arginine
What is Arginine?. The amino acid Arginine (l-Arginine HCl) is acquiring a reputation for keeping the bodys biggest muscle, the heart, in tip-top shape. In addition to the heart, Arginine can also needed to increase protein synthesis which can in turn increase cels. Studies have found that men with low sperm counts experienced an increase in the number of sperm when they were supplemented with Arginine. How does it work?. Arginine supplements appear to reduce mildly elevated blood pressure by enhancing the synthesis of Nitric Oxide (a gas) in the cells lining the blood vessels. This helps dilate vessel walls and improves blood flow around the heart. In fact, Nitric Oxide ranks as the bodys most potent blood vessel expander. In addition, Arginine may help in treating male fertility caused by circulation problems. Where does it come from?. Arginine is an amino acid found in many foods, including dairy products, meat, poultry, fish, nuts and chocolate. Safety / Side Effects. Arginine has so far ...
Asymmetric dimethylarginine endogenous inhibition of nitric oxide synthase causes differential vasculature effects - Add...
Asymmetric dimethylarginine endogenous inhibition of nitric oxide synthase causes differential vasculature effects - Add Comment #878171
An endogenous inhibitor of nitric oxide synthase regulates endothelial adhesiveness for monocytes | JACC: Journal of the...
The current study confirms previous observations of our group and others that oxidized lipoprotein increases the endothelial release of ADMA and that ADMA reduces the synthesis of NO while increasing the generation of superoxide anion. However, in this study it took rather high concentrations (10 μm) of exogenous ADMA to have a measurable effect upon NO synthesis, superoxide anion generation and endothelial adhesiveness. This concentration of ADMA is at the upper range of plasma ADMA levels observed in disease (2 to 10 μm).. However, chronic exposure to the lower levels of ADMA observed in disease states appears to have similar effects on endothelial biology as does short-term exposure to the higher dose used in this study. Specifically, we and others have demonstrated that the two to three-fold increase in plasma ADMA observed in hypercholesterolemic individuals is associated with reductions in endothelium dependent vasodilation and urinary nitrate excretion-effects that are reversed by ...
Agmatine Sulfate Powder 100 Grams - BeamZen
Agmatine can regulate nitric oxide levels, which leads to the pump often felt at the gym after taking pre workout supplements containing this ingredient.*. Citrulline is another supplement which raises plasma arginine concentration. Its unknown whether citrulline and agmatine taken together further enhance systemic nitric oxide production.* Some research suggests that they compete for uptake, so its likely not worthwhile to combine arginine and citrulline. Other supplements that do not go well with agmatine include creatine, DAA, and Yohimbine. Read more about these interactions at Examine.com.. ...
Plasma concentrations of asymmetric dimethylarginine are increased in patients with type 2 diabetes mellitus
Plasma concentrations of asymmetric dimethylarginine are increased in patients with type 2 diabetes mellitus Academic Article Article ...
Cant Cum On L-arginine | LPSG
Ive been taking L-ARGININE for about a month now about 2000mg a day, and at first the effects were great. Rock hard erections, shorter recovery time...
Does Arginine Make You Gain Weight Effects Creatine Side ~ Portal-Info-Site
Read on and youll be able t answer the question Is too much salt bad for you? Does Arginine Make You Gain Weight Effects Creatine Side Disruptions in Blood Sugar Levels - Low Blood Sugar/Hypoglycemia: can indicate problems with the liver or kidneys and can lead to Effect of oral l-arginine supplementation on blood pressure: A an erection longer than 4 hours free pills ed samples meta-analysis of randomized double-blind placebo-controlled trials e) As it helps in vasodilation it also prevents abnormal blood clotting to a significant extent . V-12 by SAN incorporates 4 the same way that N-Acetyl-L-Glutamine is a major improvement over regular glutamine Nitroarginine is a significant advancement past regular l-arginine. Directions For Beverly Int. L arginine for fioids - Gnld Products For Fioid - Buy Fioids Product on Alibaba.. Arginine plays a role in the synthesis of hormones Does Arginine Make You Gain Weight Effects Creatine Side such as insulin and growth hormone and The strain showed a ...
cth-mainz.de | cth » Professuren & Arbeitsgruppen » Prof. Wild
Lüneburg N, Lieb W, Zeller T, Chen MH, Maas R, Carter AM, Xanthakis V, Glazer NL, Schwedhelm E, Seshadri S, Ikram MA, Longstreth WT Jr, Fornage M, König IR, Loley C, Ojeda FM, Schillert A, Wang TJ, Sticht H, Kittel A, König J, Benjamin EJ, Sullivan LM, Bernges I, Anderssohn M, Ziegler A, Gieger C, Illig T, Meisinger C, Wichmann HE, Wild PS, Schunkert H, Psaty BM, Wiggins KL, Heckbert SR, Smith N, Lackner K, Lunetta KL, Blankenberg S, Erdmann J, Münzel T, Grant PJ, Vasan RS, Böger RH. Genome-Wide Association Study of L-Arginine and Dimethylarginines Reveals Novel Metabolic Pathway for Symmetric Dimethylarginine. Circ Cardiovasc Genet. 2014 ...
Douglas Labs L-Arginine
L-Arginine capsules contain 700 mg pure, crystalline L-arginine. L-arginine is a conditionally essential dibasic amino acid. It is needed for tissue protein synthesis and ammonia detoxification via urea acid cycle. L-arginine can either be used for glucose synthesis or catabolized to produce energy via the tricarboxylic acid cycle. L-arginine is required for the synthesis of creatine phosphate which functions as a carrier of readily available energy for contractile work in muscles. L-arginine is also a precursor of polyamines, including putrescine, spermine and spermidine that act as physiological growth regulators of cell proliferation, and are involved in the stabilization of cell membranes and cell organelles. L-arginine is a potent stimulator of insulin, glucagon, and growth hormone release, and functions as a representative signal to the endocrine system that dietary protein ingestion has taken place.
L-Arginine - Learn About L-Arginine - Buy L-Arginine Supplements - Nutrition Express
Low prices on L-Arginine! Improve heart health, lower blood pressure and speed up wound healing*. L-arginine is an amino acid shown to have many benefits on health. Studies have shown significant improvement of symptoms as well as objective measurements of heart function. L-arginine improves the bioavailability of nitric oxide (NO), a naturally occurring substance in the body.
L-Arginine and Nitric Oxide: What You Need to Know | Dr Nibber
arolinska Institute in Sweden found that NO could be generated from nitrates that were abundant in green leafy vegetables like spinach, lettuce, kale and especially beetroot. This requires the activation of the NOx 3,2,1 pathway to be fully operational. In effect, nitrates consumed in foods are reduced by oral bacteria into nitrites and then in the stomach into NO.. Since then, other research groups have joined the effort to uncover nitrates amazing ability to generate NO that is quicker and much more efficient than the previously known pathway via the amino acid l-arginine. Unlike, l-arginine which is only operational in conditions with good oxygen and is dependent on the proper functioning of a family of enzymes called nitric oxide synthases (NOS), nitrates offer an alternate pathway (albeit a backup) for the body to generate the versatile NO molecule quickly at low doses and in low oxygen conditions! The latter is an important factor especially during a number of pathologies where oxygen is ...
Citrulline: a potential immunomodulator in sepsis. - Science Exchange
BACKGROUNDSepsis leads to a complex systemic response of cytokines (both pro- and anti-inflammatory) and more recently recognized adipokine mediators. Endothelial nitric oxide (NO) may be a key component in regulating this response, but the pharmacologic manipulation of endothelial NO via L-arginine supplementation or inhibitors has provided inconsistent clinical data related to outcomes. These failures are related to the metabolism of L-arginine in the liver, toxicity of L-arginine, and asymmetric dimethylarginine inhibition, all of which may explain the arginine paradox. L-citrulline (CIT) offers a potentially valuable means of supplementing arginine and therefore impacting favorably NO availability. The goal of this study was to determine whether CIT supplementation altered the systemic response of mediators and cytokines in a rat model of sepsis with varying degrees of severity.METHODSSepsis was induced with 2 models of cecal ligation and puncture (CLP) of varying severity in Wistar rats. ...
Top 3 Wound Healing Tips - MTI
Much like remaining calm, doctors have also noted that a proper diet is effective at improving your rate of healing. Similarly, people should try to add more nutrients and vitamins into their daily health routine. The amino acid arginine features a compound called nitric oxide, and increased levels have proven to bolster both your bloods oxygenation levels and overall circulation. Glutamine - one of the 20 or so essential amino acids that create the human genetic code - provides your body with substrate, a molecule thats essential for tissue growth. Both amino acids can be found in supplements like L-glutamine and L-arginine, but always consult with a physician before you begin take any supplement.. Article provided by Advanced Tissue. Click here to visit their website. ...
Chronic L-arginine supplementation improves endothelial cell vasoactive functions in hypercholesterolemic and atherosclerotic...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
cSNP analysis of the ABCA1 gene: Common variant lowers riskfor coronary artery disease
Haydens investigation of variation within the ABCA1 gene was prompted by the finding that Tangier disease, characterized by almost a complete absence of HDL-C, is caused by a defect in ABCA1. This led Haydens team to search for other variants of the cholesterol transporter gene that might modify the risk of heart disease.. The researchers identified 16 single nucleotide polymorphisms (SNPs) a specific type of variation within the coding region of the ABCA1 gene. Of these, 10 caused amino acid changes in the protein, which have the potential to alter the function of the transporter. In the R219K variant the amino acid arginine is replaced with lysine. The authors note that the SNPs tend to be concentrated away from the functional regions of the transporter protein. They suggest that this may be why most variations seem to exert such minor effects. Defective ABC transporters have been implicated in a number of diseases: ABCA1 in Tangier disease, ABCC7, which transports chloride ions, in cystic ...
Sexual Energy | Boomer Wellness
Nitric Oxide is a free form of gas produced by the cells in our body. Symbolized as NO, Nitric Oxide contains one atom of Nitrogen and one of Oxygen. It is produced when enzymes in the body break down the amino acid Arginine, and is mainly used for intra-cellular communication. So, read on if you are […]. ...
MT PLATINUM 100% L-ARGININE 1000MG 100 CAPLETS - FITDXB
Its Called The Pump, And For Years, Bodybuilders And Other Athletes Have Relied On L-Arginine To Enhance These Muscle-Swelling Pumps To Feed Their Muscles The Nutrients They Need To Perform, Recover And Grow Bigger For Sleeve-Stretching Pumps That Are Absolutely Unreal!. New Platinum 100% L-Arginine Delivers A Full 1,000mg Dose Of L-Arginine For Rapid Uptake And Dispersal In Your Body ...
The L-Arginine in Eggs | Healthy Eating | SF Gate
The amino acid L-arginine is of particular interest to bodybuilders and people seeking alternative treatments for sexual dysfunction. Along with other aminos, it contributes to muscle growth and ...
L-arginine in Diet May Improve DMD Gut Function, Mouse Study Suggests
A diet that included the amino acid L-arginine improved gut function in a mouse model of DMD, a study found, suggesting the same may be true for people.
Polyethylenes.org - Molecules That Save Lives
Natural forms of amino acid L-arginine is found in animal based products like red meat, poultry, fish, and dairy as well as plant-based products such as nuts, seeds, oats, beans and wheat germ are good plant-based sources of arginine. Andthere are plenty of manufacturers who make it in their labs.. Mayo Clinic lists therapeutic dosages of L-arginine for anything from altitude sickness and asthma to coronary artery disease (CAD), diabetes, exercise performance and transplants.. L-arginine converts in the body to nitric oxide that is a vasodilator.. It is used to improve the health of the heart and blood vessels. Dilatation of blood vessels can relieve the symptoms of pain related to blood restrictions. Keeping blood vessels pliable could also contribute to improved health of those who suffer from congestive heart failure and high blood pressure.. Your blood vessels are like a garden hose. When you squeeze them, the stream narrows, and the pressure goes up. When you relax them, the stream widens, ...
157-06-2|D-(-)-Arginine ChemVia Mall, Custom Chemical Mall
Visit ChemVia,you find CAS NO.157-06-2,D-(-)-Arginine specification and current price;you can buy 157-06-2,D-(-)-Arginine reagent online,and you can buy D-(-)-Arginine on bulk underline.
Biomarker | SimulTOF Systems
In the simplest case, complex samples are spotted directly onto a MALDI plate. If the sample consists of proteins that are digested with trypsin, then the most abundant proteins can be identified by Peptide Mass Fingerprinting (PMF) starting from high resolution reflectron spectra. SimulTof Systems has developed special PMF software that takes advantage of the well-known preferential detection of arginine-containing peptides that makes PMF practical even for whole tissue preparations, so long as most proteins are largely intact. Differences between preparations can be determined using peak list information loaded into spreadsheets like Excel, and / or exported to 3rd party software like R, SpotFire or GeneSpring. The following poster describes this workflow starting from chicken meat preparations obtained from a grocery store, which is a model system for studying myosin heavy chain differences in human muscle biopsy samples.. ...
ALZFORUM | NETWORKING FOR A CURE
Rudich P, Snoznik C, Watkins SC, Monaghan J, Pandey UB, Lamitina ST. Nuclear localized C9orf72-associated arginine-containing dipeptides exhibit age-dependent toxicity in C. elegans ...
L-Arginine - 21st Century - Swanson Health Products
Shop the best L-Arginine - 21st Century products at Swanson Health Products. Trusted since 1969, we offer trusted quality and great value on L-Arginine - 21st Century products.
The administration of L-arginine during the acute and interictal periods may represent a potential new therapy for this ... 2007). "MELAS and L-arginine therapy". Mitochondrion. 7 (1-2): 133-9. doi:10.1016/j.mito.2006.11.006. PMID 17276739. Hirata K, ... 2007). "Effect of l-arginine on synaptosomal mitochondrial function". Brain Development. 30 (4): 238-45. doi:10.1016/j.braindev ...
... catalyses the production of nitric oxide from L-arginine. 2L-arginine + 3NADPH + 3 H+ + 4O2 -> 2citrulline + 2NO• + 4H2O + ...
Arginine can be methylated once (monomethylated arginine) or twice (dimethylated arginine). Methylation of arginine residues is ... The existence of arginine demethylases that could reverse arginine methylation is controversial. The name of this modification ... It is a mark that indicates the di-methylation at the 17th arginine residue of the histone H3 protein. In epigenetics, arginine ... Arginine methylation plays a major role in gene regulation because of the ability of the PRMTs to deposit key activating ( ...
Vasopressin receptor antagonist
Decaux, G; Soupart, A; Vassart, G (2008). "Non-peptide arginine-vasopressin antagonists: the vaptans". Lancet. 371 (9624): 1624 ...
Citrullinemia type I
The beta-lactamases are produced in the cytoplasm and translocated to the periplasmic space by twin-arginine translocation ... M. catarrhalis utilizes the twin-arginine translocation pathway (TAT pathway) for the transport of folded proteins across the ... Balder R, Shaffer TL, Lafontaine ER (June 2013). "Moraxella catarrhalis uses a twin-arginine translocation system to secrete ... Palmer T, Berks BC (June 2012). "The twin-arginine translocation (Tat) protein export pathway". Nature Reviews. Microbiology. ...
Slocum, Robert D. (August 2005). "Genes, enzymes and regulation of arginine biosynthesis in plants". Plant Physiology and ...
Creatine phosphate shuttle
In some vertebrates, arginine phosphate plays a similar role. Creatine supplementation is an effective method of increasing an ...
Eight unphosphorylated repeats of arginine and serine in the RS domain take a helical form with arginine on the outside to ... Methylation of arginine residues in the RRM may also contribute to the export of SR proteins out of the nucleus. SR proteins ... SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose ... The methylation and charge of arginine residues in the RRM domain also contributes to the export of SR proteins associated with ...
"GCGR is identified by newborn screening of elevated arginine". Molecular Genetics and Metabolism Reports. 17: 46-52. doi: ...
Malcolm Davenport Milne
Asatoor, A. M.; Harrison, B. D.; Milne, M. D.; Prosser, D. I. (1972). "Intestinal absorption of an arginine-containing peptide ...
Distal convoluted tubule
Arginine vasopressin receptor 2 is also expressed in the DCT. Thiazide diuretics inhibit Na+/Cl− reabsorption from the DCT by ...
Arginine phosphorylation in many Gram-positive bacteria marks proteins for degradation by a Clp protease. Recent studies from ... The arginine phosphorylation system, which is widely distributed across Gram-positive bacteria, appears to be functionally ... These anionic residues can interact with cationic residues such as lysine and arginine to form salt bridges, stable non- ... ATP-binding cassette transporter Arginine phosphorylation by McsB kinase marks proteins for degradation by a Clp protease. ...
Liu H, Drew P, Gaugler AC, Cheng Y, Visner GA (June 2005). "Pirfenidone inhibits lung allograft fibrosis through L-arginine- ...
... with arginine-8 of AVT changed to leucine), mesotocin (arginine-8 changed to isoleucine), and isotocin (glutamine-4 changed to ... Arginine vasotocin (AVT), a hormone produced by neurosecretory cells within the posterior pituitary gland (neurohypophysis) of ... ISBN 3-540-58961-9. Ervin MG, Leake RD, Ross MG, Calvario GC, Fisher DA (May 1985). "Arginine vasotocin in ovine fetal blood, ... Ervin MG, Amico JA, Leake RD, Ross MG, Robinson AG, Fisher DA (1988). "Arginine vasotocin and a novel oxytocin-vasotocin-like ...
Arginine methylation. What was said above of the chemistry of lysine methylation also applies to arginine methylation, ... Arginine citrullination. Enzymes called peptidylarginine deiminases (PADs) hydrolyze the imine group of arginines and ... and some protein domains-e.g., Tudor domains-can be specific for methyl arginine instead of methyl lysine. Arginine is known to ... Salt bridges and hydrogen bonds between side chains of basic amino acids (especially lysine and arginine) and phosphate oxygens ...
Borriello, G; Richards, L; Ehrlich, GD; Stewart, PS (January 2006). "Arginine or nitrate enhances antibiotic susceptibility of ...
Isolates in the S. sanguinis group are arginine and esculin positive. Like members of the S. mitis group, they are negative for ... S. salivarius group organisms are positive for acetoin production and are esculin positive but are negative for arginine ... Isolates in this group are negative for acetoin production, arginine, esculin, and mannitol and are sorbitol fermentation ... They do not hydrolyze arginine but are positive for acetoin production, esculin hydrolysis, and mannitol and sorbitol ...
Amino acid synthesis
The gene product of argR, ArgR an aporepressor, and arginine as a corepressor affect the operon of arginine biosynthesis. The ... Proline and arginine are derived from glutamate. Serine, formed from 3-phosphoglycerate, is the precursor of glycine and ... Arginine synthesis also utilizes negative feedback as well as repression through a repressor encoded by the gene argR. ... In addition, the amino acids arginine, cysteine, glycine, glutamine, histidine, proline, serine, and tyrosine are considered ...
Shao A, Hathcock JN (2008). "Risk assessment for the amino acids taurine, L-glutamine and L-arginine". Regulatory Toxicology ...
... s like acetopine and nopaline can also be formed in normal callus and plant tissue as a result of arginine metabolism. ... Nopaline Nopaline (N2-(D-1,3-dicarboxypropyl)-L-arginine) was first isolated from crown gall tumors. It is the head member of ... Octopine Octopine (N2-(D-1-carboxyethyl)-L-arginine) is the first opine discovered in 1927 in octopus muscle and later in crown ... It was also found in certain nontransformed plant tissues as a result of arginine metabolism Nopalinic acid Nopalinic acid ( ...
The guanidine group of arginine residues condense with malondialdehyde to give 2-aminopyrimidines. Human ALDH1A1 aldehyde ...
There has been one case study reported of asthma in a human stemming from an allergy associated with the arginine kinase ... Bobolea, Irina; Barranco, Pilar; Pastor-Vargas, Carlos; Iraola, Victor; Vivanco, Fernando; Quirce, Santiago (2011). "Arginine ...
L-arginine and conception - Conception
ink that L-arginine might be the most disputed conception aid. Some sources state it improves quantity of eggs but decreases ... L-arginine and conception. I think that L-arginine might be the most disputed conception aid. Some sources state it improves ... The authors found that the women who did not receive L-arginine supplmentation had the same L-arginine follicular levels as the ... L-arginine converts in the body to nitric oxide. Nitric oxide causes vasodilation, and that may help to increase blood flow to ...
The L-arginine-nitric oxide pathway. - PubMed - NCBI
The L-arginine-nitric oxide pathway.. Moncada S1, Higgs A.. Author information. 1. Wellcome Research Laboratories, Beckenham, ...
Improve Your Health With L-Arginine - InfoBarrel
Get a clearer profile of the numerous health benefits of l-arginine. ... L-arginine is a functional amino acid that helps you to maintain youthful vigor. It supports cardiovascular health, muscle mass ... L-Arginine. L-Arginine is a functional amino acid. A functional amino acid may be loosely defined as an amino acid with notable ... L-Arginine can open up these cholesterol-clogged arteries and improve blood flow. L-arginine can also prevent or slow down the ...
Solgar L-Arginine 500mg - 50 vegicaps at health4youonline.com
Health supplement with L-Arginine which is essential for,tissue regeneration. Used for of burns, trauma and sepsis. ... L-Arginine is an important component in tissue generation and regeneration.. *L-Arginine is highly concentrated in the skin and ... Solgar L-Arginine 500mg. *L-Arginine is essential in muscle metabolism because it provides a vehicle for transport, storage and ... Each Solgar L-Arginine Vegetable Capsule provides:. L-Arginine 500 mg. Encapsulated with these natural ingredients:. ...
Arginine glutamate - Wikipedia
Arginine alpha-ketoglutarate - Wikipedia
Arginine alpha-ketoglutarate (AAKG) is a salt of the amino acid arginine and alpha-ketoglutaric acid. It is marketed as a ... plasma L-arginine, nitric oxide metabolites, and asymmetric dimethyl arginine after resistance exercise". International Journal ... Willoughby, DS; Boucher T; Reid J; Skelton G; Clark M (Aug 2011). "Effects of 7 days of arginine-alpha-ketoglutarate ... Wax, B; A Kavazis; H Webb; S Brown (2012). "Acute L-arginine alpha ketoglutarate supplementation fails to improve muscular ...
Protein arginine methyltransferase 5 - Wikipedia
histone-arginine N-methyltransferase activity. • protein-arginine N-methyltransferase activity. • protein-arginine omega-N ... peptidyl-arginine methylation, to symmetrical-dimethyl arginine. • chromatin organization. • histone arginine methylation. ... "Entrez Gene: PRMT5 protein arginine methyltransferase 5".. *^ Stopa N, Krebs JE, Shechter D (June 2015). "The PRMT5 arginine ... Protein arginine N-methyltransferase 5 is an enzyme that in humans is encoded by the PRMT5 gene. PRMT5 symmetrically ...
Fat Burning Via Arginine - YouTube
DESCRIPTION: The arginine content of nuts may explain their metabolism boosting effects, though in a list of the top food ... Arginine Does Not Work Unless....... - Duration: 6:20. Tim Muriello 13,076 views ... L-Arginine Benefits For Bodybuilding? - Duration: 4:25. Sean Nalewanyj 104,294 views ... Nobel Prize winner talks about ARGI+ L-arginine supplement - Duration: 4:53. Katalin Hidvegi 70,978 views ...
L-Arginine: MedlinePlus Supplements
L-Arginine: learn about effectiveness, usual dosage, and drug interactions on MedlinePlus ... Arginine Hydrochloride, Di-Arginine Malate, Di-Arginine Orotate, Di-L-Arginine-L-Malate, Dl-Arginine, L-Arginina, L-Arginine ... Arginine, Arginine Aspartate, Arginine Ethyl Ester, Arginine Ethyl Ester Dihydrochloride, Arginine Ethyl Ester HCl, Arginine ... L-Arginine L-Pyroglutamate, L-Arginine Pyroglutamate, L-Arginine Taurinate, Malate de Di-Arginine, Orotate de Di-Arginine, R- ...
L arginine ed treatment 2014
... l arginine ed treatment 2014, best male enhancement extenders ucla, ed sheeran tattoo paw ... Arginine capsules allow for a quick and convenient way for bodybuilders and athletes to provide extra essential arginine pre- ... L-Arginine is an essential amino acid necessary in the synthesis of nitric oxide, hormone production, optimal function of the ... Arginine facilitates the creation of nitric oxide, which helps the blood carry more nutrients and more oxygen to working ...
Arginine - Everything2.com
Arginine isnt assembled by the body, so it... ... Sources of arginine include: *brown rice *nuts *popcorn * ... Arginine isnt assembled by the body, so it must be ingested, especially by people at a young age. ... structural formula for arginine: _ _ , H H , + , \ / , , N H H H H O , , \\ \ / \ / // , , C -- N -- C -- C -- C -- C -- C ... H -- N H H H H N O -- H , , / / \ , , H arginine H H , ,_ C6H15N4O2+ _, ...
Arginine:glycine amidinotransferase deficiency: MedlinePlus Genetics
Arginine:glycine amidinotransferase deficiency is an inherited disorder that primarily affects the brain. Explore symptoms, ... glycine, arginine, and methionine. Specifically, arginine:glycine amidinotransferase controls the first step of the process. In ... Children with arginine:glycine amidinotransferase deficiency may not gain weight and grow at the expected rate (failure to ... The prevalence of arginine:glycine amidinotransferase deficiency is unknown. The disorder has been identified in only a few ...
Arginine | Define Arginine at Dictionary.com
Arginine definition, an essential amino acid, C 6 H 14 N 4 O 2 : the free amino acid increases insulin secretion and is ... arginine in Medicine Expand. arginine ar·gi·nine (ärjə-nēn). n. Abbr. Arg An amino acid obtained from the hydrolysis or ... First recorded in 1885-90, arginine is from the German word Arginin, a name unexplained by its originators ... In vertebrates, the basis for the phosphoric acid is creatine, whereas invertebrates have arginine instead. ...
EWG Skin Deep® | What is ARGININE
ARGININE || Skin Deep® Cosmetics Database | EWG
ARGININE; L- (+) -ARGININE; L-ARGININE; L-ORNITHINE, N5- (AMINOIMINOMETHYL) - ... About ARGININE: Arginine is a naturally occurring amino acid.. Function(s): Fragrance Ingredient; Hair Conditioning Agent; Skin ... Synonym(s): L-A-AMINO-D -GUANIDINOVALERIC ACID; L-ARGININE; L-ORNITHINE, N5- (AMINOIMINOMETHYL) -; N5- (AMINOIMINOMETHYL) - L- ...
Arginine: Good for the Heart
... or L-arginine, is one of the 20 amino acids we get from proteins. Its typically considered a semi-essential amino acid. Find ... What is arginine?. Arginine, or L-arginine, is one of 20 amino acids that you get from proteins in your diet. Its typically ... What does arginine do?. Your body uses arginine to synthesize nitric oxide. Nitric oxide helps your blood vessels relax and ... Taking arginine supplements may make some health issues worse. Always talk to your doctor before adding arginine supplements to ...
L-Arginine - iHerb
Brands A-Z Source Naturals L-Arginine Categories Supplements Amino Acids L-Arginine Categories Sports Nutrition Post-Workout ... Source Naturals, L-Arginine 4 Results (showing 1 - 4) Visit Manufacturers Website » ...
L-Arginine - iHerb
Brands A-Z Carlson Labs L-Arginine Categories Supplements Amino Acids L-Arginine Categories Sports Nutrition Post-Workout ...
Arginine N-methyltransferase 2 (A1CSW8) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Arginine (L-arginine): Heart Benefits and Side Effects
L-arginine) to improve heart health. Find out where it comes from, if supplementation works, and its possible side effects. ... Why Do We Need Arginine?. Arginine, also known as L-arginine, is involved in a number of different functions in the body. They ... What Are Arginines Heart Benefits?. In the body, the amino acid arginine changes into nitric oxide (NO). Nitric oxide is a ... Is Arginine Supplementation Safe?. In clinical trials, arginine has been used safely with minor side effects for up to three ...
L-arginine: Potential benefits, side effects, and risks
Athletes and others seek out ways to boost their L-arginine intake. It may have some other health benefits, but its use can ... Learn about some natural ways to consume L-arginine and some side effects. ... L-arginine is an amino acid that helps the body to build proteins. ... L-arginine is one of many amino acids the body needs to function properly.. Like other amino acids, L-arginine plays a role in ...
Arginase and Arginine Dysregulation in Asthma
L-Arginine Support Group - Drugs.com
Ask questions and get answers about L-Arginine. Our support group helps people share their own experience. 16 questions, 24 ... L-Arginine Patient Information at Drugs.com. *L-Arginine Information for Healthcare Professionals (includes dosage details) ... L-Arginine - Im interested in ProArgi-9 and want to know what effects the suplement will have with?. Posted 21 Dec 2010 • 1 ... L-Arginine - Is l-argenine safe to take if you have hypothyroidism caused from graves disease?. Posted 25 Jan 2010 • 1 answer ...
Remote control of γc expression by arginine methylation | Nature Immunology
Arginine methylation is a post-translational modification that controls the abundance of γc cytokine receptor on mature T cells ... 1: The butterfly effect of arginine methylation on the expression of and signaling via γc cytokine receptors.. ... Arginine methylation controls the strength of γc-family cytokine signaling in T cell maintenance *Maia Inoue ... Arginine methylation is a post-translational modification that controls the abundance of γc cytokine receptor on mature T cells ...
arginine | Journal of Bacteriology
Arginine and Citrulline Catabolic Pathways Encoded by the arc Gene Cluster of Lactobacillus brevis ATCC 367 Maria Majsnerowska ... l-Lysine Catabolism Is Controlled by l-Arginine and ArgR in Pseudomonas aeruginosa PAO1 Han Ting Chou, Mohamed Hegazy, Chung- ... Two Essential Arginine Residues in the T Components of Energy-Coupling Factor Transporters Olivia Neubauer, Anja Alfandega, ... Role of the Twin-Arginine Translocation Pathway in Staphylococcus Lalitha Biswas, Raja Biswas, Christiane Nerz, Knut Ohlsen, ...
Arginine Vasopressin Receptor | ALZFORUM
arginine - Webvision
This poster was presented today at the Association for Research in Vision and Opthalmology (ARVO) meetings in Ft. Lauderdale, Florida by W. Drew Ferrell, Lloyd Williams, Carl B. Watt, James R. Anderson, Robert E. Marc and Bryan William Jones. Full size (almost) poster can be seen here.. ...
L-Arginine: Uses, Side Effects, Interactions, Dosage, and Warning
... user ratings and products that contain L-Arginine ... Learn more about L-Arginine uses, effectiveness, possible side ... Arginine Hydrochloride, Di-Arginine Malate, Di-Arginine Orotate, Di-L-Arginine-L-Malate, Dl-Arginine, L-Arginina, L-Arginine ... L-Arginine L-Pyroglutamate, L-Arginine Pyroglutamate, L-Arginine Taurinate, Malate de Di-Arginine, Orotate de Di-Arginine, R- ... Arginine, Arginine Ethyl Ester, Arginine Ethyl Ester Dihydrochloride, Arginine Ethyl Ester HCl, Arginine HCl, ...
arginine facts, information, pictures | Encyclopedia.com articles about arginine
Make research projects and school reports about arginine easy with credible articles from our FREE, online encyclopedia and ... Arginine is one of the amino acids produced in the human body by the digestion, or hydrolysis of proteins. Arginine can also be ... "Arginine and Exercise." Better Nutrition (June 2000).. Chowienczyk, Phil and Jim Ritter. "Arginine: NO more than a simple amino ... Arginine is necessary for growth periods but not for body maintenance.. Benefits of the use of arginine as a supplement include ...
L-arginine, Nitric Oxide And Sexual Health
... and L-arginine (an essential amino acid) are critical components to your sexual health. In ... ... It needs to be emphasized that L-arginine is not a hormone and it is not testosterone. L-arginine is an essential amino acid. ... Do the methods of consumption follow the guidelines for making L-arginine available for the body? • Who is the L-arginine ... L-arginine and Its Dark Side!. Because of its huge potential to aid people, L-arginine supplements have populated the shelves ...
Arginine: Indications, Side Effects, Warnings - Drugs.com
Easy-to-read patient leaflet for Arginine. Includes indications, proper use, special instructions, precautions, and possible ... What do I need to tell my doctor BEFORE I take Arginine?. *If you are allergic to arginine; any part of arginine; or any other ... How do I store and/or throw out Arginine?. *If you need to store arginine at home, talk with your doctor, nurse, or pharmacist ... Arginine. Generic Name: Arginine (AR ji neen). Brand Name: R-Gene 10 ...
PrecursorMetabolismInfusion500mgImprovesADMAProteinsPathwayCardiovascularCitrullineSupplementation with arginineConditionally essentialErectileCONCLUSIONSDietDietary L-arginineSupplementsSynthesisImmuneSupplementationSupplementsSupplementHydrochlorideOrnithineMetabolismTabletsMethyltransferasePhosphorylated on arginine residuesCapsulesKinaseEating arginine-richCoronaryMagnesium StearateLevels of arginineAmount of arginineNitric Oxide SySoybeans2002VitroResiduesTake arginineHumansArginasePerindopril arginineExceed the body'sDeficiencyBody'sCellularBlood flowGrams of L-arginineCombination of l-arginineGlutamateIntravenous arginineDeiminated in vivoDietaryIngredientsNaturallyConvertsEffectivenessOral L-arginineSources of arginineEffects of arginineSynthesis of arginineNonessential amino acidVivoKidneyBody
- L-arginine is also a precursor of polyamines, including putrescine, spermine and spermidine that act as physiological growth regulators of cell proliferation, and are involved in the stabilization of cell membranes and cell organelles. (healthystars.sg)
- L-Arginine is a precursor to nitric oxide, which causes dilation of the blood vessels, thereby improving circulation. (hsnstore.com)
- L-Arginine also plays an important role in urea metabolism and immune system health. (biotechpharmacal.com)
- Over eight weeks, the team supplemented the diets of both groups of mice with L-arginine, L-citrulline - another amino acid that is converted to L-arginine within the body - or L-alanine (involved in sugar metabolism, immunity, and energy for muscle tissue). (musculardystrophynews.com)
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- Studies researching the influence of taking 500mg (up to2000mg) of L-arginine every day are still ongoing and there are no definiteresults yet. (steadyhealth.com)
- If you're in need of an added dose of arginine, Nutri Essentials L-Arginine 500mg dietary supplement can provide you with the nutritional support that you need to achieve your fitness, health and wellness goals. (nutriessentials.com)
- Our arginine 500mg formula only requires you to take one easy-to-swallow capsule to get the optimum- most frequently recommended dosage of L-Arginine. (nutriessentials.com)
- Bodybuilders use L-arginine to increase muscle mass, becauseit improves formation of proteins and production of creatine, also. (steadyhealth.com)
- In the body, L-arginine changes into nitric oxide (NO). Nitric oxide is a powerful neurotransmitter that helps blood vessels relax and also improves circulation. (nutriessentials.com)
- Objective: This study investigated the impact of stress on effectors of the L-arginine/nitric oxide (NO) system including the endogenous inhibitor asymmetric dimethylarginine (ADMA). (edu.au)
- Serum samples for determination of L-arginine, NO metabolites, ADMA, and symmetric dimethylarginine (SDMA) were obtained at rest and during stress exposure. (edu.au)
- Ethnicity-by-psychological distress interaction was observed for resting levels of ADMA (p = .002), SDMA (p = .038), and L-arginine (p = .048). (edu.au)
- Ethnicity-by-psychological distress interaction for stress responses was found for L-arginine/ADMA ratio (p = .027). (edu.au)
- Concentrations of L-Arginine and the methylarginines ADMA and symmetric dimethylarginine (SDMA) were determined from plasma samples. (imperial.ac.uk)
- The L-arginine-nitric oxide pathway. (nih.gov)
- The amino acid L-Arginine plays a number of important roles in your body to help your immune and cardiovascular systems function at their bests. (nutriessentials.com)
- L-Arginine is known to aid in the efficient functioning of our cardiovascular system as arginine may help improve blood flow in the arteries of the heart. (nutriessentials.com)
- Yet diets with L-arginine or L-citrulline lessened contractions in the mid- and distal colons of mdx mice. (musculardystrophynews.com)
- Dietary supplementation with L-arginine - but not L-citrulline - normalized resting diameter in the distal colon of mdx mice compared with controls. (musculardystrophynews.com)
Supplementation with arginine1
- Research indicates that supplementation with arginine also promotes tissue recovery and repair after exercise, injury, or surgery. (biotechpharmacal.com)
- L-arginine is a conditionally essential dibasic amino acid. (healthystars.sg)
- Diverse studies show that 3000mg of Arginine is the optimal amount to support vascular health and associated with Erectile Function. (hsnstore.com)
- Conclusions: The L-arginine/NO system is affected by psychosocial distress with higher susceptibility in black South Africans. (edu.au)
- These findings in mdx mice show that dietary L-arginine supplementation can improve colonic motility [and] have therapeutic relevance for DMD, with potential for alleviating GI discomfort, improving clinical care, and enhancing quality of life," they said. (musculardystrophynews.com)
- The purpose of this study was to evaluate the effectiveness of dietary L-arginine in prevention of methotrexate (MTX) induced intestinal damage in a rat model and to investigate the mechanisms involved in the stimulating effect of arginine on enterocyte turnover including its effects on cell proliferation and cell death via apoptosis. (biomedcentral.com)
- L-arginine can either be used for glucose synthesis or catabolized to produce energy via the tricarboxylic acid cycle. (healthystars.sg)
- L-arginine is required for the synthesis of creatine phosphate which functions as a carrier of readily available energy for contractile work in muscles. (healthystars.sg)
- L-Arginine is known to have amazing effects when it comes to strengthening our immune system. (nutriessentials.com)
- Studies examining arginine supplementation have shown significant improvements in men suffering from erectile dysfunction (ED), boosting both performance and confidence. (amazonaws.com)
- The problem with this study is that the patients weren't supplemented with L-arginine, so it doesn't correlate to say that supplementation is a bad thing due to these results. (bellaonline.com)
- Is Arginine Supplementation Safe? (webmd.com)
- The February 2005 issue of "Nutrition in Clinical Practice" reports that arginine supplementation has been shown to enhance wound healing both in rodents and in humans. (livestrong.com)
- oral L-arginine supplementation in poor responder patients may improve ovarian response, endometrial receptivity and pregnancy rate. (bellaonline.com)
- Amin HJ, Zamora SA, McMillan DD et al (2002) Arginine supplementation prevents necrotizing enterocolitis in the premature infant. (springer.com)
- A study recently published in the American College of Sports Medicine shows oral supplementation with L-Citrulline before exercise may help reduce gastrointestinal problems associated with physical strain by increasing L-Arginine levels - resulting in better NO production - that provide increased circulation to abdominal and intestinal areas. (prweb.com)
- And a related study on rats and rabbits published in September in Biochemical and Biophysical Research Communications showed L-Citrulline plus L-Arginine supplementation caused a more rapid increase in plasma L-Arginine levels and marked enhancement of NO bioavailability, including plasma cGMP concentrations, than with dosage with the single amino acids. (prweb.com)
- The scientists were encourage to find no adverse effects from supplementation with L-Arginine and L-Citrulline in the dosages specified in the instruction. (verichipcorp.com)
- All these factors let us resume that proper L-Arginine and L-Citrulline supplementation can manage and even treat penile endothelial dysfunction and help in dealing with ED. (verichipcorp.com)
- Arginine supplementation (5.6-12.6 grams per day) has been used successfully in double-blind trials to treat CHF.A double-blind trial has also found that arginine supplementation (5 grams three times daily) improves kidney function in people with CHF. (peacehealth.org)
- Arginine supplementation was found to be particularly effective at improving ED in men with abnormal nitric oxide metabolism. (peacehealth.org)
- In this study we wanted to test the hypothesis based on previous observations that an arginine rich food supplementation might enhance clinical improvement in patients with smear positive tuberculosis and if this effect could be due to increased nitric oxide production. (clinicaltrials.gov)
- Scientific studies have shown that supplementing Citrulline along with Arginine helps maintain nitrogen balance better than supplementation with Arginine alone. (fredmeyer.com)
- L-arginine supplements provide effective support for men suffering from erectile dysfunction and remain a very popular, safe and affordable alternative to prescription ED medication. (amazonaws.com)
- If you eat a well-balanced diet, you probably don't need to take arginine supplements. (healthline.com)
- For some people, taking arginine supplements may actually increase their risk of health problems. (healthline.com)
- But arginine supplements are also available in the form of oral tablets or injections. (healthline.com)
- Always consult your doctor before trying any new supplements, including supplemental arginine. (healthline.com)
- Your doctor may recommend eating arginine-rich foods or taking arginine supplements if you have certain conditions that affect your blood vessels and heart. (healthline.com)
- For example, they recommend taking arginine supplements to help treat coronary artery disease , peripheral vascular disease , or erectile dysfunction . (healthline.com)
- In other cases, arginine supplements may increase your risk of heart problems. (healthline.com)
- According to the Mayo Clinic , arginine supplements may have benefits for people with certain cardiovascular conditions. (healthline.com)
- Arginine supplements may also have perks for people with peripheral vascular disease (PVD). (healthline.com)
- On the other hand, the Mayo Clinic warns that taking arginine supplements after a heart attack may make matters worse. (healthline.com)
- Until the potential safety issues have been addressed, avoid taking arginine supplements after a heart attack. (healthline.com)
- If you have a history of heart disease, ask your doctor about the potential benefits and risks of arginine supplements. (healthline.com)
- According to the Mayo Clinic , some research suggests that arginine supplements may help improve blood flow through your body. (healthline.com)
- Due to these purported benefits, doctors sometimes prescribe arginine supplements to treat ED. In fact, early studies have found that arginine supplements may help alleviate ED in men with low nitrate levels. (healthline.com)
- A study reported in Urology also found that oral supplements of L-citrulline may help treat mild to moderate ED. Your body converts L-citrulline into L-arginine. (healthline.com)
- Recent studies suggest that arginine supplements may have other benefits too. (healthline.com)
- For example, a research review published in the Asia Pacific Journal of Clinical Nutrition suggests that arginine supplements may have positive effects on your immune system. (healthline.com)
- Another study, reported in Biology of Sport , found that taking arginine supplements before exercise increased the time it took for male wrestlers to become exhausted. (healthline.com)
- Other research, reported in Endocrinology , found that arginine supplements can help improve glucose tolerance in mice. (healthline.com)
- But if you have a history of certain health conditions, your doctor may recommend taking arginine supplements. (healthline.com)
- Taking arginine supplements may make some health issues worse. (healthline.com)
- Always talk to your doctor before adding arginine supplements to your routine. (healthline.com)
- Supplements that combine arginine with other amino acids, such as ornithine and lysine , are purported to assist in muscle-building exercises by minimizing body fat and maximizing muscle tone. (encyclopedia.com)
- Other new research was finding that arginine supplements worked as an effective anticoagulant, but unlike aspirin and other anticoagulants, could prevent clotting without increasing stroke risk. (encyclopedia.com)
- Arginine supplements as an alternative medicine therapy are normally taken in either tablet or capsule form. (encyclopedia.com)
- Long-term effects of arginine supplements have not yet been determined. (encyclopedia.com)
- A doctor may suggest arginine supplements for those suffering from conditions such as coronary heart disease, congestive heart failure, erectile dysfunction, high blood pressure or clogged arteries according to Healthline. (reference.com)
- One of the more common supplements marketed to help treat ED is L-arginine. (healthline.com)
- However, L-arginine is often combined with other supplements, which have different results. (healthline.com)
- A study in the Journal of Sex and Marital Therapy fou