Arginase

Hyperargininemia

Arginine

Ornithine

Aminocaproates

Ureohydrolases

Nitric Oxide

Nitric Oxide Synthase Type II

Ornithine Carbamoyltransferase

Nitric Oxide Synthase

Urea

Boronic Acids

Cationic Amino Acid Transporter 2

Citrulline

Arginase

D-arginase

METAP2

Argininemia

NOS1

ARG1 (gene)

Stanley Cohen (biochemist)

PEGylation

ARG2

Intestinal permeability

Homoarginine

Canavanine

Interleukin-13 receptor

Transition state analog

Urea cycle

Pirfenidone

FLOT2

Ureohydrolase

Macrophage-activating factor

Ethylenediaminetetraacetic acid

Manganese in biology

Manganese

Insulin-degrading enzyme

John Yudkin

Helicobacter pylori

Adipose tissue macrophages

Regulatory macrophages

Hyperammonemia

Ornithine

Canaline

Involves elevated arginase1

• Obesity-induced vascular inflammation involves elevated arginase activity. (bvsalud.org)

ARG14

• Mutations in the ARG1 gene cause arginase deficiency. (medlineplus.gov)
• The ARG1 gene provides instructions for making an enzyme called arginase. (medlineplus.gov)
• Arginase-1, encoded by the ARG1 gene, is a cytosolic metalloenzyme expressed predominantly in hepatocytes which plays a key role in the urea cycle by catalyzing the hydrolysis of arginine to ornithine and urea. (femtopath.com)
• In addition to classical biomarkers, liver injury was assessed by cytokeratin 18 (CK18) cleaved form, high-mobility group box 1, arginase 1 (ARG1), microRNA 122 (miR-122), and glutamate dehydrogenase. (nih.gov)

Inhibitor2

• These effects of HFHS, except for weight gain and hyperglycemia , were prevented or reduced in mice lacking EC-A1 or treated with the arginase inhibitor 2-(S)-amino-6-boronohexanoic acid (ABH). (bvsalud.org)
• To better evaluate this effect, we chronically administered an arginase inhibitor, norvaline, to triple-transgenic and wild-type mice, and applied an advanced immunohistochemistry approach with several biomarkers and bright-field microscopy. (nih.gov)

Enzyme4

• The molecular weight of native arginase, estimated by gel filtration on a Sephadex G-150 column, was 122 500 ± 2500 and those of subunits of the enzyme, estimated by SDS-PAGE and gel filtration on a Sephadex G-100 column, were 44 000 ± 1000 and 34 500 ± 500. (tubitak.gov.tr)
• Results: Arginase was found to have an alkaline pH optima(~9.5) with little enzyme activity at physiological pH. (medscimonit.com)
• Arginase (ARG), an enzyme implicated in many cardiovascular diseases, can compete with nitric oxide (NO) synthase for their common substrate, L-arginine. (elsevierpure.com)
• They have shown for the first time in a mouse model of tough-to-treat optic nerve trauma, that removing the enzyme arginase 2, which increases with injury, decreases neuron death in the retina as well as the degeneration of nerve fibers that connect neurons to each other and ultimately the brain. (nih.gov)

Deficiency8

• Arginase deficiency is an inherited disorder that causes the amino acid arginine (a building block of proteins) and ammonia to accumulate gradually in the blood. (medlineplus.gov)
• Arginase deficiency usually becomes evident by about the age of 3. (medlineplus.gov)
• In some affected individuals, signs and symptoms of arginase deficiency may be less severe, and may not appear until later in life. (medlineplus.gov)
• Arginase deficiency belongs to a class of genetic diseases called urea cycle disorders. (medlineplus.gov)
• In people with arginase deficiency, arginase is damaged or missing, and arginine is not broken down properly. (medlineplus.gov)
• The accumulation of ammonia and arginine are believed to cause the neurological problems and other signs and symptoms of arginase deficiency. (medlineplus.gov)
• Hyperargininemia due to liver arginase deficiency. (medlineplus.gov)
• Sun A, Crombez EA, Wong D. Arginase Deficiency. (medlineplus.gov)

Hyperargininemia1

• examine the neurological changes associated with hyperargininemia using an arginase-1-deficient mouse. (jci.org)

Induces1

• Silica exposure induces arginase I expression in rat lung. (cdc.gov)

Ornithine2

• For instance, in non-cancerous cells arginine is synthesized in cells from citrulline via argininosuccinate synthase 1 (ASS1) and argininosuccinate lyase in the urea cycle 8 , and metabolized by arginase 1 to produce urea and ornithine. (nature.com)
• Increased arginase can also provide ornithine for synthesis of polyamines via ornithine decarboxylase (ODC) and proline/collagen via ornithine aminotransferase (OAT), leading to vascular cell proliferation and collagen formation, respectively.We hypothesized that elevated arginase activity is involved in Ang II-induced arterial thickening, fibrosis, and stiffness and that limiting its activity can prevent these changes. (elsevierpure.com)

Hepatic2

• Anti-Arginase-1 is highly specific for hepatocytes, and is therefore a sensitive and specific marker of benign and malignant hepatic tumors. (femtopath.com)
• They find that dysmyelination occurs shortly after birth but can be corrected by restoring hepatic arginase-1 via adeno-associated virus-mediated gene delivery, suggested a potential therapeutic approach that merits further study. (jci.org)

Liver2

• Comparison of arginase activity with CEA and Ca 19-9 in liver metastases of colorectal cancer]. (nih.gov)
• 6. [Serum arginase activity in patients with liver cirrhosis and hepatocellular carcinoma]. (nih.gov)

Enzymes1

• Manganese is a cofactor for many enzymes, including manganese superoxide dismutase, arginase, and pyruvate carboxylase [ 1 , 2 ]. (nih.gov)

Synthesis1

• However, arginase has failed to inhibit NO synthesis. (medscimonit.com)

Significance2

• Better model system to study functional significance of arginase II. (nih.gov)
• 4. L-arginine as a factor increasing arginase significance in diagnosis of primary and metastatic colorectal cancer. (nih.gov)

Tissue6

• The arginase was partially purified from cattle rumen tissue and its properties were investigated and thereafter compared with those of other tissue arginases. (tubitak.gov.tr)
• The presence of a single anionic form of arginase in cattle rumen tissue was demonstrated by DEAE-sephacel chromatography. (tubitak.gov.tr)
• Cattle rumen tissue arginase contains only one isoenzyme. (tubitak.gov.tr)
• Arginase isolated from cattle rumen tissue may be arginase A_4 or A_2 form when it is compared with those of other tissue arginase isoenzymes. (tubitak.gov.tr)
• ADI, unlike arginase, was effective in lowering extracellular arginine in tissue culture media and inhibit NO production by the murine macrophage cell line N-9 in response to gamma interferon and LPS stimulation. (medscimonit.com)
• MCTR3 reprograms arthritic monocytes to upregulate Arginase-1 and exert pro-resolving and tissue-protective functions in experimental arthritis. (logosbio.com)

Activity4

• This was accompanied by increased arginase activity and A1 expression in vascular ECs and increased expression of tumor necrosis factor -α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), interleukin-10 ( IL-10 ), vascular cell adhesion molecule-1 ( VCAM-1 ), and intercellular adhesion molecule-1 ( ICAM-1 ) mRNA and protein in both VAT and ECs. (bvsalud.org)
• Limiting arginase activity is a possible therapeutic means of controlling obesity -induced vascular and VAT inflammation . (bvsalud.org)
• Level of arginase activity in peritoneal macrophages isolated from the transgenic mice also was also unchanged. (nih.gov)
• 1. Serum arginase activity in postsurgical monitoring of patients with colorectal carcinoma. (nih.gov)

Mice1

• Our previous results indicated a potential effect of arginase inhibition, with norvaline, on various aspects of neurogenesis in triple-transgenic mice. (nih.gov)

Studies1

• Our previous studies showed that arginase 1 (A1) in endothelial cells (ECs) is critically involved in obesity -induced vascular dysfunction. (bvsalud.org)

Role1

• Useful to study the role of arginase II gene in endothelium. (nih.gov)

Mouse1

• The cover image shows restoration of basal dendritic arbors of layer V pyramidal tract neurons in an AAV-treated arginase-1-deficient mouse. (jci.org)

Levels1

• ADI had more than 1000 fold higher affinity for arginine (Km ~ 30 µM for ADI vs ~45 mM for arginase), and was able to lower arginine levels to a much greater extent than arginase. (medscimonit.com)

Cell1

• Material/Methods: The murine macrophage cell line N-9 was treated with either arginase or arginine deiminase to determine the effect on intracellular and extracellular arginine and nitric oxide production. (medscimonit.com)

People with arginase3

• In people with arginase deficiency, arginase is damaged or missing, and arginine is not broken down properly. (medlineplus.gov)
• People with arginase deficiency may also have developmental delay, loss of developmental milestones, and intellectual disability. (nih.gov)
• People with Arginase deficiency may be placed on a very low-protein diet with the help of a registered metabolic dietitian. (nih.gov)

Called urea cycle dis1

• Arginase deficiency belongs to a class of genetic diseases called urea cycle disorders. (medlineplus.gov)

Deficiency usually2

• Arginase deficiency usually becomes evident by about the age of 3. (medlineplus.gov)
• If untreated, arginase deficiency usually progresses to severe spasticity, loss of ambulation, complete loss of bowel and bladder control, and severe intellectual disability. (nih.gov)

Induced Pluri1

• Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells. (medscape.com)

Competes2

• Arginase competes with eNOS for L-arginine and has been implicated in the endothelial dysfunction. (nih.gov)
• Arginase competes with NOS for L-arginine to produce urea and ornithine , limiting NO production . (bvsalud.org)

Inhibition2

• Our previous results indicated a potential effect of arginase inhibition, with norvaline, on various aspects of neurogenesis in triple-transgenic mice. (nih.gov)
• Furthermore, AGEs impair vascular function that can be reversed by arginase inhibition. (bvsalud.org)

Hyperammonemia1

• Arginase deficiency in untreated individuals is characterized by episodic hyperammonemia of variable degree that is infrequently severe enough to be life threatening or to cause death. (nih.gov)

Therapeutic Target1

• Therefore, AGEs may be pivotal in arginase deleterious effects in diabetic VD, providing a novel therapeutic target. (bvsalud.org)

Gene4

• Rescue of the Functional Alterations of Motor Cortical Circuits in Arginase Deficiency by Neonatal Gene Therapy. (medscape.com)
• Useful to study the role of arginase II gene in endothelium. (nih.gov)
• Interestingly, blood cell gene expression in the apoE-/- fed 60% fat showed an 11-fold increase in arginase II (AII) and no increase in arginase I (AI). (cdc.gov)
• Increased arginase gene expression in the blood and tissue (apoE-/- only) are likely associated with the development of atherosclerosis. (cdc.gov)

Amino acid2

• Arginase deficiency is an inherited disorder that causes the amino acid arginine (a building block of proteins) and ammonia to accumulate gradually in the blood. (medlineplus.gov)
• As stated previously, plasma arginine levels can be low under conditions of stress, for example following surgery, when this amino acid is excessively catabolized by arginase. (medscape.com)

Proteins1

• High levels of LDH appear to indirectly indicate that two other proteins, hemoglobin and arginase, have broken out of red blood cells. (nih.gov)

Genetic1

• Arginase deficiency is a genetic disease, which means that it is caused by one or more genes not working correctly. (nih.gov)

Conclusion2

• In conclusion, AGEs increase arginase activity probably through the ERK1/2/ p38 MAPK pathway due to increased arginase I expression. (bvsalud.org)
• In conclusion, increased circulating arginase activity and reduced arginine are likely the result of liver and/ or tissue injury. (cdc.gov)

Atherosclerosis1

• Therefore we studied systemic changes in arginase and arginine metabolism in a model of atherosclerosis. (cdc.gov)

Regulation1

• however, AGEs' role in arginase regulation is unknown. (bvsalud.org)

Severe1

• In some affected individuals, signs and symptoms of arginase deficiency may be less severe, and may not appear until later in life. (medlineplus.gov)

Symptoms1

• The accumulation of ammonia and arginine are believed to cause the neurological problems and other signs and symptoms of arginase deficiency. (medlineplus.gov)

Human2

• The human arginases and arginase deficiency. (medscape.com)
• 6. Arginase in human urogenital tumors. (nih.gov)

Expression2

• Arginase deficiency with lethal neonatal expression: evidence for the glutamine hypothesis of cerebral edema. (medscape.com)
• Immunodetection of arginase revealed MGA-induced protein expression for arginase I. In aortic rings, MGA pretreatment impaired acetylcholine (ACh)-induced vasorelaxation , which was reversed by ABH. (bvsalud.org)

Model1

• Better model system to study functional significance of arginase II. (nih.gov)