Arginase: A ureahydrolase that catalyzes the hydrolysis of arginine or canavanine to yield L-ornithine (ORNITHINE) and urea. Deficiency of this enzyme causes HYPERARGININEMIA. EC 3.5.3.1.Hyperargininemia: A rare autosomal recessive disorder of the urea cycle. It is caused by a deficiency of the hepatic enzyme ARGINASE. Arginine is elevated in the blood and cerebrospinal fluid, and periodic HYPERAMMONEMIA may occur. Disease onset is usually in infancy or early childhood. Clinical manifestations include seizures, microcephaly, progressive mental impairment, hypotonia, ataxia, spastic diplegia, and quadriparesis. (From Hum Genet 1993 Mar;91(1):1-5; Menkes, Textbook of Child Neurology, 5th ed, p51)Arginine: An essential amino acid that is physiologically active in the L-form.Ornithine: An amino acid produced in the urea cycle by the splitting off of urea from arginine.Aminocaproates: Amino derivatives of caproic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the amino caproic acid structure.UreohydrolasesNitric Oxide: A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.Nitric Oxide Synthase Type II: A CALCIUM-independent subtype of nitric oxide synthase that may play a role in immune function. It is an inducible enzyme whose expression is transcriptionally regulated by a variety of CYTOKINES.Ornithine Carbamoyltransferase: A urea cycle enzyme that catalyzes the formation of orthophosphate and L-citrulline (CITRULLINE) from CARBAMOYL PHOSPHATE and L-ornithine (ORNITHINE). Deficiency of this enzyme may be transmitted as an X-linked trait. EC 2.1.3.3.Nitric Oxide Synthase: An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.Urea: A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.Boronic Acids: Inorganic or organic compounds that contain the basic structure RB(OH)2.Cationic Amino Acid Transporter 2: A high-affinity, low capacity system y+ amino acid transporter with strong similarity to CATIONIC AMINO ACID TRANSPORTER 1. The two isoforms of the protein, CAT-2A and CAT-2B, exist due to alternative mRNA splicing. The transporter has specificity for the transport of ARGININE; LYSINE; and ORNITHINE.CitrullinePeriodicals as Topic: A publication issued at stated, more or less regular, intervals.Access to Information: Individual's rights to obtain and use information collected or generated by others.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Peer Review, Research: The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.Helicobacter pylori: A spiral bacterium active as a human gastric pathogen. It is a gram-negative, urease-positive, curved or slightly spiral organism initially isolated in 1982 from patients with lesions of gastritis or peptic ulcers in Western Australia. Helicobacter pylori was originally classified in the genus CAMPYLOBACTER, but RNA sequencing, cellular fatty acid profiles, growth patterns, and other taxonomic characteristics indicate that the micro-organism should be included in the genus HELICOBACTER. It has been officially transferred to Helicobacter gen. nov. (see Int J Syst Bacteriol 1989 Oct;39(4):297-405).PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Helicobacter Infections: Infections with organisms of the genus HELICOBACTER, particularly, in humans, HELICOBACTER PYLORI. The clinical manifestations are focused in the stomach, usually the gastric mucosa and antrum, and the upper duodenum. This infection plays a major role in the pathogenesis of type B gastritis and peptic ulcer disease.BooksUrea Cycle Disorders, Inborn: Rare congenital metabolism disorders of the urea cycle. The disorders are due to mutations that result in complete (neonatal onset) or partial (childhood or adult onset) inactivity of an enzyme, involved in the urea cycle. Neonatal onset results in clinical features that include irritability, vomiting, lethargy, seizures, NEONATAL HYPOTONIA; RESPIRATORY ALKALOSIS; HYPERAMMONEMIA; coma, and death. Survivors of the neonatal onset and childhood/adult onset disorders share common risks for ENCEPHALOPATHIES, METABOLIC, INBORN; and RESPIRATORY ALKALOSIS due to HYPERAMMONEMIA.Nylons: Polymers where the main polymer chain comprises recurring amide groups. These compounds are generally formed from combinations of diamines, diacids, and amino acids and yield fibers, sheeting, or extruded forms used in textiles, gels, filters, sutures, contact lenses, and other biomaterials.Proline: A non-essential amino acid that is synthesized from GLUTAMIC ACID. It is an essential component of COLLAGEN and is important for proper functioning of joints and tendons.Sprue, Tropical: A chronic malabsorption syndrome, occurring mainly in residents of or visitors to the tropics or subtropics. The failed INTESTINAL ABSORPTION of nutrients from the SMALL INTESTINE results in MALNUTRITION and ANEMIA that is due to FOLIC ACID deficiency.Helminths: Commonly known as parasitic worms, this group includes the ACANTHOCEPHALA; NEMATODA; and PLATYHELMINTHS. Some authors consider certain species of LEECHES that can become temporarily parasitic as helminths.Strongylida Infections: Infections with nematodes of the order STRONGYLIDA.Tuberculosis: Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.Nematospiroides dubius: A species of intestinal nematode parasites which occur most commonly in mice. Infection is by ingesting larvae. This particular species is used extensively in immunological research.Tuberculosis, Pulmonary: MYCOBACTERIUM infections of the lung.Coinfection: Simultaneous infection of a host organism by two or more pathogens. In virology, coinfection commonly refers to simultaneous infection of a single cell by two or more different viruses.Fasciola hepatica: A species of helminth commonly called the sheep liver fluke. It occurs in the biliary passages, liver, and gallbladder during various stages of development. Snails and aquatic vegetation are the intermediate hosts. Occasionally seen in man, it is most common in sheep and cattle.Fascioliasis: Liver disease caused by infections with parasitic flukes of the genus FASCIOLA, such as FASCIOLA HEPATICA.Fasciola: A genus of trematode liver flukes of the family Fasciolidae. Two species of this genus are F. hepatica and F. gigantica. The parasites are found in the liver and gallbladder and associated ducts in mammals and occasionally man. F. gigantica occurs rarely in man.Opisthorchis: A genus of trematode liver flukes of the family Opisthorchidae. It consists of the following species: O. felineus, O. noverca (Amphimerus noverca), and O. viverrini. The intermediate hosts are snails, fish, and AMPHIBIANS.Opisthorchiasis: Infection with flukes of the genus Opisthorchis.Dicrocoelium: A genus of trematode liver flukes of the family Dicrocoeliidae which includes the species dendriticum and hospes. It occurs in the biliary passages or liver of many vertebrates including man. The intermediate hosts are mainly mollusks but occasionally ants.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Hemiptera: A large order of insects characterized by having the mouth parts adapted to piercing or sucking. It is comprised of four suborders: HETEROPTERA, Auchenorrhyncha, Sternorrhyncha, and Coleorrhyncha.Biological Availability: The extent to which the active ingredient of a drug dosage form becomes available at the site of drug action or in a biological medium believed to reflect accessibility to a site of action.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Carotid Artery Injuries: Damages to the CAROTID ARTERIES caused either by blunt force or penetrating trauma, such as CRANIOCEREBRAL TRAUMA; THORACIC INJURIES; and NECK INJURIES. Damaged carotid arteries can lead to CAROTID ARTERY THROMBOSIS; CAROTID-CAVERNOUS SINUS FISTULA; pseudoaneurysm formation; and INTERNAL CAROTID ARTERY DISSECTION. (From Am J Forensic Med Pathol 1997, 18:251; J Trauma 1994, 37:473)Carotid Arteries: Either of the two principal arteries on both sides of the neck that supply blood to the head and neck; each divides into two branches, the internal carotid artery and the external carotid artery.Tunica Intima: The innermost layer of an artery or vein, made up of one layer of endothelial cells and supported by an internal elastic lamina.Catheterization: Use or insertion of a tubular device into a duct, blood vessel, hollow organ, or body cavity for injecting or withdrawing fluids for diagnostic or therapeutic purposes. It differs from INTUBATION in that the tube here is used to restore or maintain patency in obstructions.

Expression of arginase II and related enzymes in the rat small intestine and kidney. (1/943)

Arginase, which catalyzes the conversion of arginine to urea and ornithine, and consists of a liver-type (arginase I) and a non-hepatic type (arginase II). Arginine is also used for the synthesis of nitric oxide and creatine phosphate, while ornithine is used for the synthesis of polyamines and proline, and thus collagen. Arginase II mRNA and protein are abundant in the intestine (most abundant in the jejunum and less abundant in the ileum, duodenum, and colon) and kidney of the rat. In the kidney, the levels of arginase II mRNA do not change appreciably from 0 to 8 weeks of age. In contrast, arginase II mRNA and protein in the small intestine are not detectable at birth, appear at 3 weeks of age, the weaning period, and their levels increase up to 8 weeks. On the other hand, mRNAs for ornithine aminotransferase (OAT), ornithine decarboxylase, and ornithine carbamoyltransferase (OCT) are present at birth and their levels do not change much during development. Arginase II is elevated in response to a combination of bacterial lipopolysaccharide, dibutyryl cAMP, and dexamethasone in the kidney, but is not affected by these treatments in the small intestine. Immunohistochemical analysis of arginase II, OAT, and OCT in the jejunum revealed their co-localization in absorptive epithelial cells. These results show that the arginase II gene is regulated differentially in the small intestine and kidney, and suggest different roles of the enzyme in these two tissues. The co-localization of arginase II and the three ornithine-utilizing enzymes in the small intestine suggests that the enzyme is involved in the synthesis of proline, polyamines, and/or citrulline in this tissue.  (+info)

Arginase from human full-term placenta. (2/943)

Arginase was purified about 1800-fold from extracts of human full-term placenta; the enzyme appeared to be homogenous by disc electrophoresis and molecular-sieve chromatography. The mol. wt. determination by gel filtration and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis yielded a value of 70000 for the most pure and the partially purified enzyme. The human placenta arginase is a metalloenzyme with an optimum pH of 9.1. The Km for L-arginine is 27 mM. L-Ornithine and L-lysine show competitive inhibition with Ki values of 6.3 and 14 mM respectively.  (+info)

Glucocorticoids mediate the enhanced expression of intestinal type II arginase and argininosuccinate lyase in postweaning pigs. (3/943)

Arginine metabolism is enhanced in the small intestine of weanling pigs, but the molecular mechanism(s) involved is not known. The objectives of this study were to determine the following: 1) whether glucocorticoids play a role in induction of intestinal arginine metabolic enzymes during weaning; 2) whether the induction of enzyme activities was due to increases in corresponding mRNA levels; and 3) the identity of the arginase isoform(s) expressed in the small intestine. Jejunum was obtained from 29-d-old weaned pigs that were or were not treated with 17-beta-hydroxy-11beta-(4-dimethylaminophenyl)17alpha-(prop- 1-ynyl)es tra-4,9-dien-3-one (RU486, an antagonist of glucocorticoid receptors), or from age-matched suckling pigs. Activities and mRNA levels for type I and type II arginases, argininosuccinate synthase (ASS) and argininosuccinate lyase (ASL) were determined. Activities of arginase, ASL and ASS increased by 635, 56 and 106%, respectively, in weanling pigs, compared with suckling pigs. RU486 treatment attenuated the increase in arginase activity by 74% and completely prevented the ASL induction in weanling pigs, but had no effect on ASS activity. Pig intestine expresses both type I and type II arginases. On the basis of immunoblot analyses, there was no significant difference in levels of intestinal type I arginase among these three groups of pigs, indicating that changes in arginase activity were due only to type II arginase. The mRNA levels for type II arginase and ASL increased by 135 and 198%, respectively, in weanling pigs compared with suckling pigs, and this induction was completely prevented by RU486. In contrast, ASS mRNA levels did not differ between suckling and weanling pigs. These results suggest that intestinal type II arginase, ASS and ASL are regulated differentially at transcriptional and post-translational levels and that glucocorticoids play a major role in the induction of type II arginase and ASL mRNAs in the small intestine of weanling pigs.  (+info)

Metabolic capacity for L-citrulline synthesis from ammonia in rat isolated colonocytes. (4/943)

Ammonia is present at high concentration in the colon lumen and is considered a colon cancer suspect. Furthermore, ammonia usually eliminated by the liver in the ornithine cycle is considered highly toxic to cerebral function when present in excess in the blood plasma. Therefore, the metabolic pathways involved in ammonia metabolism in colonocytes were studied in the present study. Rat colonocytes were found equipped with low carbamoylphosphate synthase I activity, high ornithine carbamoyltransferase and arginase activities and low argininosuccinate synthase activity. High (10 and 50 mmol/l) NH4Cl concentrations but not low concentrations (1 and 5 mmol/l) were found able to increase respectively 3- and 10-fold the conversion of radioactive L-arginine to L-citrulline. In contrast, very low capacity for L-citrulline conversion to L-arginine is found in colonocytes. It is concluded that an incomplete ornithine cycle is operative in colonocytes which results in ammonia stimulated L-citrulline production. The contribution of this metabolic pathway in relation to ammonia detoxication by colonocytes is discussed.  (+info)

Regulation of the genes for arginase isoforms and related enzymes in mouse macrophages by lipopolysaccharide. (5/943)

Arginase exists in two isoforms, the hepatic (arginase I) and extrahepatic types (arginase II). Arginase I is markedly induced in rat peritoneal macrophages and rat tissues in vivo by bacterial lipopolysaccharide (LPS). In contrast, both arginase I and arginase II are induced in LPS-activated mouse peritoneal macrophages. In the present study, expression of arginase isoforms and related enzymes was studied in mouse tissues in vivo and in peritoneal macrophages with RNA blot and immunoblot analyses and enzyme assay. When mice were injected intraperitoneally with LPS, inducible nitric oxide synthase (iNOS) and arginase II were induced early in the lung and spleen. mRNAs for argininosuccinate synthase (AS) and ornithine decarboxylase (ODC) were also induced early. In comparison, arginase I was induced later in the lung. Early induction of iNOS, arginase II, AS, ODC, and cationic amino acid transporter 2 and late induction of arginase I were observed in LPS-activated peritoneal macrophages. These results indicate that the genes for the two arginase isoforms are regulated differentially. Possible roles of the arginase isoforms in the regulation of nitric oxide production and in polyamine synthesis are discussed.  (+info)

Biochemical and functional profile of a newly developed potent and isozyme-selective arginase inhibitor. (6/943)

An increase in arginase activity has been associated with the pathophysiology of a number of conditions, including an impairment in nonadrenergic and noncholinergic (NANC) nerve-mediated relaxation of the gastrointestinal smooth muscle. An arginase inhibitor may rectify this condition. We compared the effects of a newly designed arginase inhibitor, 2(S)-amino-6-boronohexanoic acid (ABH), with the currently available N(omega)-hydroxy-L-arginine (L-HO-Arg), on the NANC nerve-mediated internal anal sphincter (IAS) smooth-muscle relaxation and the arginase activity in the IAS and other tissues. Arginase caused an attenuation of the IAS smooth-muscle relaxations by NANC nerve stimulation that was restored by the arginase inhibitors. L-HO-Arg but not ABH caused dose-dependent and complete reversal of N(omega)-nitro-L-arginine-suppressed IAS relaxation that was similar to that seen with L-arginine. Both ABH and L-HO-Arg caused an augmentation of NANC nerve-mediated relaxation of the IAS. In the IAS, ABH was found to be approximately 250 times more potent than L-HO-Arg in inhibiting the arginase activity. L-HO-Arg was found to be 10 to 18 times more potent in inhibiting the arginase activity in the liver than in nonhepatic tissues. We conclude that arginase plays a significant role in the regulation of nitric oxide synthase-mediated NANC relaxation in the IAS. The advent of new and selective arginase inhibitors may play a significant role in the discrimination of arginase isozymes and have important pathophysiological and therapeutic implications in gastrointestinal motility disorders.  (+info)

Th1/Th2-regulated expression of arginase isoforms in murine macrophages and dendritic cells. (7/943)

Activated murine macrophages metabolize arginine by two alternative pathways involving the enzymes inducible NO synthase (iNOS) or arginase. The balance between the two enzymes is competitively regulated by Th1 and Th2 T helper cells via their secreted cytokines: Th1 cells induce iNOS, whereas Th2 cells induce arginase. Whereas the role of macrophages expressing iNOS as inflammatory cells is well established, the functional competence of macrophages expressing arginase remains a matter of speculation. Two isoforms of mammalian arginases exist, hepatic arginase I and extrahepatic arginase II. We investigated the regulation of arginase isoforms in murine bone marrow-derived macrophages (BMMPhi) in the context of Th1 and Th2 stimulation. Surprisingly, in the presence of either Th2 cytokines or Th2 cells, we observe a specific induction of the hepatic isoform arginase I in BMMPhi. Induction of arginase I was shown on the mRNA and protein levels and obeyed the recently demonstrated synergism among the Th2 cytokines IL-4 and IL-10. Arginase II was detectable in unstimulated BMMPhi and was not significantly modulated by Th1 or Th2 stimulation. Similar to murine BMMPhi, murine bone marrow-derived dendritic cells, as well as a dendritic cell line, up-regulated arginase I expression and arginase activity upon Th2 stimulation, whereas arginase II was never detected. In addition to revealing the unexpected expression of arginase I in the macrophage/monocyte lineage, these results uncover a further intriguing parallelism between iNOS and arginase: both have a constitutive and an inducible isoform, the latter regulated by the Th1/Th2 balance.  (+info)

Carbon and nitrogen repression of arginine catabolic enzymes in Bacillus subtilis. (8/943)

Specific activities of arginase and ornithine aminotransferase, inducible enzymes of arginine catabolism in Bacillus subtilis 168, were examined in cells grown with various carbon and nitrogen sources. Levels of these enzymes were similar in arginine-induced cultures whether glucose or citrate was the carbon source (in contrast to histidase), suggesting that carbon source catabolite repression has only limited effect. In media with combinations of nitrogen sources, glutamine strongly repressed induction of these enzymes by proline or arginine. Ammonium, however, only repressed induction by proline and had no effect on induction by arginine. These effects correlate with generation times in media containing these substances as sole nitrogen sources: growth rates decreased in the order glutamine-arginine-ammonium-proline. Similar phenomena were observed when glutamine or ammonium were added to arginine- or proline-grown cultures, or when arginine or proline were added to glutamine- or ammonium-grown cultures. In the latter cases, an additional feature was apparent, namely a surprisingly long transition between steady-state enzyme levels. The results are compared with those for other bacteria and for eucaryotic microorganisms.  (+info)

TY - JOUR. T1 - Oxidative species increase arginase activity in endothelial cells through the RhoA/Rho kinase pathway. AU - Chandra, S.. AU - Romero, M. J.. AU - Shatanawi, A.. AU - Alkilany, A. M.. AU - Caldwell, R. B.. AU - Caldwell, R. William. PY - 2012/1/1. Y1 - 2012/1/1. N2 - Background and Purpose NO produced by endothelial NOS is needed for normal vascular function. During diabetes, aging and hypertension, elevated levels of arginase can compete with NOS for available l-arginine, reducing NO and increasing superoxide (O 2 .-) production via NOS uncoupling. Elevated O 2 .- combines with NO to form peroxynitrite (ONOO -), further reducing NO. Oxidative species increase arginase activity, but the mechanism(s) involved are not known. Our study determined the mechanism involved in peroxynitrite and hydrogen peroxide-induced enhancement in endothelial arginase activity. We hypothesized that oxidative species increase arginase activity through PKC-activated RhoA/Rho kinase (ROCK) pathway. ...
The present study demonstrates that arginase plays a fundamental role in vascular growth after injury. Arterial injury stimulates arginase activity and arginase I protein expression in the vessel wall, and local arginase inhibition leads to a significant decline in VSMC DNA synthesis and reduced intimal thickening. In addition, this study shows that arginase promotes the entry of VSMCs into the cell cycle. Arginase inhibition or arginase I knockdown arrests VSMCs in G0/G1, and this is associated with the induction of the cyclin-dependent protein kinase inhibitor, p21. These findings illustrate a critical role for arginase in cell cycle progression and identify arginase I as a novel therapeutic target for the treatment of occlusive vascular disorders.. In the present study, we are the first to demonstrate that arginase plays an integral role in the remodeling response after arterial injury. Balloon injury of rat carotid arteries resulted in a sustained increase in arginase activity in the vessel ...
Arginase, an enzyme within the urea cycle in the liver, is also found in many other cells and tissues, including the lung. Arginase is present in 2 isoforms: arginase I, the hepatic isoform; and arginase II, the extrahepatic isoform; each of which is encoded by a distinct gene.. The expression and function of arginase I in macrophages, hepatocytes, and vascular smooth muscle cells, is stimulated by lipopolysaccharide (LPS), IL-13, altered oxygen tension, and balloon dilatation of coronary arteries. The activation and expression of endothelial arginase II can also be induced by a variety of vascular insults.. Arginase competitively inhibits nitric oxide synthase (NOS) via use of the common substrate L-arginine. Inhibition of arginase activity enhances a variety of parameters relevant to allergic airways disease, possibly by altering NO homeostasis. Arginase inhibition actively augments NO production and has beneficial effects on normal cardiac function and on vascular dysfunction typical of ...
1HQ5: CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH S-(2-BORONOETHYL)-L-CYSTEINE, AN L-ARGININE ANALOGUE
Effect of nor-NOHA on arginase activity and amino acid levels. (A) Significant arginase inhibition was observed in cell lysates of CL-19 cultures treated with n
In the present study we demonstrated that IL-13, a Th2 cell-derived cytokine, is a potent arginase activator, and its induction of arginase contributes significantly to the suppression of NO production in LPS-activated macrophages. The increase in arginase activity is a result of de novo synthesis of arginase I mRNA and protein. Studies on the signaling molecules involved in arginase activation show that a surge in intracellular cAMP and the subsequent activation of PKA are obligatory for arginase induction. In addition, tyrosine kinases and p38 MAPK play a role in IL-13-induced arginase activation. To provide a perspective on our observations and conclusions, the results from the present study are discussed below in reference to previous findings regarding the signaling pathways involved in arginase activation and NO regulation by arginase.. In the present study despite the basal level of arginase I gene expression being detected in resting macrophages, arginase protein expression and enzyme ...
BioAssay Systems Arginase Inhibitor Kit (IARG-100) screens for arginase inhibitors using a chromogen that forms a colored complex specifically with urea. The color (430nm) is proportional to the arginase activity. Percent inhibition is determined by comparing treated samples to an untreated control.
There are no specific protocols for Recombinant Human Liver Arginase protein (ab95487). Please download our general protocols booklet
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Liver Arginase小鼠多克隆抗体(ab89184)可与人样本反应并经WB实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Human embryonic stem cells (hESCs) can proliferate extensively in culture and give rise to progeny of the three germ layers. Several reports suggested that mouse and hESCs may attenuate immune responses. In this study, we focused on the mechanism by which hESCs inhibit T cell responses. Using coculture experiments, we demonstrate that hESCs inhibit cytokine secretion and T cell proliferation in response to potent T cell activators. Furthermore, we show that hESCs downmodulate the TCR-associated CD3-ζ chain. These effects are maintained when hESCs are replaced by their conditioned media and can be restored by the addition of l-arginine to hESC-conditioned media or by treatment of hESCs with a specific arginase inhibitor. Moreover, we show arginase-I expression and activity in hESCs. We further demonstrate that mouse ESCs (mESCs) similarly inhibit T cell activation via arginase I, suggesting an evolutionary conserved mechanism of T cell suppression by ESCs. In addition, we demonstrate that ...
Approach and Results-The HDAC inhibitor trichostatin A increased levels of Arg2 mRNA, protein, and activity in both human aortic endothelial cells and mouse aortic rings. These changes occurred in both time- and dose-dependent patterns and resulted in Arg2-dependent endothelial dysfunction. Trichostatin A and the atherogenic stimulus oxidized low-density lipoprotein enhanced the activity of common promoter regions of Arg2. HDAC inhibition with trichostatin A also decreased endothelial nitric oxide, and these effects were blunted by arginase inhibition. Nonselective class I HDAC inhibitors enhanced Arg2 expression, whereas the only selective inhibitor that increased Arg2 expression was mocetinostat, a selective inhibitor of HDACs 1 and 2. Additionally, mouse aortic rings preincubated with mocetinostat exhibited dysfunctional relaxation. Overexpression of HDAC2 (but not HDAC 1, 3, or 8) cDNA in human aortic endothelial cells suppressed Arg2 expression in a concentration-dependent manner, and siRNA ...
TY - JOUR. T1 - In vivo whole body and organ arginine metabolism during endotoxemia (sepsis) is dependent on mouse strain and gender. AU - Luiking, Y.C.. AU - Hallemeesch, M.M.. AU - Vissers, Y.L.J.. AU - Lamers, W.H.. AU - Deutz, N.E.P.. PY - 2004/1/1. Y1 - 2004/1/1. N2 - In vivo whole body and organ arginine metabolism during endotoxemia (sepsis) is dependent on mouse strain and gender.Luiking YC, Hallemeesch MM, Vissers YL, Lamers WH, Deutz NE.Maastricht University, Department of Surgery, The Netherlands.Arginine metabolism involves various organs such as the kidney, the intestines, and the liver, which act together in an interorgan axis. Major pathways for arginine production are protein breakdown and de novo arginine production from citrulline; disposal of arginine is mainly used for protein synthesis or used by the enzymes arginase and nitric oxide synthase (NOS). To assess in vivo organ arginine metabolism under normal conditions and during endotoxemia we used a mouse model, and analyzed ...
2PHO: Crystal structure of human arginase I complexed with thiosemicarbazide reveals an unusual thiocarbonyl mu-sulfide ligand in the binuclear manganese cluster.
BioAssay Systems Arginase Assay Kit (DARG-100) measures arginase activity. A chromogen forms a colored complex with urea produced in the reaction. The color (430nm) is proportional to the arginase activity. Samples: enzyme preparations, serum, plasma & tissue culture. Detection range: 0.3 - 800 U/L.
BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.
Arginase Mouse anti-Human, Clone: sl6arg, eBioscience™ 25μg; Unlabeled Arginase Mouse anti-Human, Clone: sl6arg, eBioscience™ Primary Antibodies Ar to Az
Small-molecule arginase inhibitors are currently described as promising therapeutic agents for the treatment of variety of diseases, including cancer. Arginase vaccination could induce Th1 inflammation at tumor sites where regulatory myeloid cells otherwise prevent lymphocyte infiltration....
PHILADELPHIA - Researchers at the University of Pennsylvania and other institutions have identified an enzyme that appears to play a key role in bringing on sexual dysfunction in both men and women - and a second molecule that can just as easily yank the offending enzyme out of commission. The findings, which carry the possibility of new treatments for sexual disorders, are scheduled to appear in two papers in the March 13 issue of Biochemistry, a peer-reviewed journal of the American Chemical Society, the worlds largest scientific society. Led by Penn chemist David W. Christianson, the team found that the enzyme arginase can effectively short-circuit a biochemical pathway critical to male sexual arousal. But unlike remedies developed expressly for erectile dysfunction, which have proven disappointing in clinical trials with women, treatments that home in on arginase may offer hope for both sexes. There is intense interest in new targets for sexual dysfunction therapy, said Christianson, the ...
Cardiovascular complications of diabetes are a leading cause of morbidity and mortality. Vascular endothelial dysfunction (VED) is strongly implicated..
The spread of tumorous cells and the formation of metastases in other organs are the main cause of cancer relapse. This study has also enabled the identification of a molecule (arginase 1) which is strongly expressed in white blood cells in the presence of a cancer and which promotes the formation of metastases. The inhibition of this molecule stimulates the activation of the anti-tumorous T-lymphocytes and greatly reduces the formation of metastases.. These results have potential implications for breast cancer patients. On the one hand, antiangiogenic treatments could be used in combination with existing immunotherapy treatments. On the other, arginase inhibitors are already at a clinical development stage and could be tested for the prevention of relapses.. C. Secondini, O. Coquoz, L. Spagnuolo, L. Ciarloni, T. Spinetti, S. Peyvandi, F. Botta, C. Bourquin and C. Rüegg. Arginase inhibition suppresses lung metastasis in the 4T1 breast cancer model independently of the immunomodulatory and ...
l-Arginine did not produce the anticipated reversal of insulin resistance produced byl-NAME, but, rather,l-arginine by itself caused insulin resistance (48.8 ± 8.2%) (Fig. 5).l-NAME not only blocks NOS but also blocks arginine uptake across the hepatocyte plasma membrane (8).l-Arginine is metabolized by NOS to NO and by arginase to urea andl-ornithine (6). Because the liver has a very high arginase activity, it is possible that mostl-arginine administered is converted to l-ornithine by the liver, although l-arginine can reverse the vascular effects ofl-NAME in the liver (12).l-Arginine also causes release of growth hormone (7, 14) and glucagon; both hormones reduce insulin sensitivity. This may explain why we could not reverse insulin resistance caused by l-NAME withl-arginine and whyl-arginine caused insulin resistance.. Reduction in blood flow to the nerves in diabetes leads to neuropathy (3, 4, 9, 17, 25) and has been suggested to result from a decrease in NO production in the vasculature ...
l-Arg is a nonessential amino acid that plays a central role in several biological systems including the immune response. l-Arg is metabolized by arginase I, arginase II, and inducible nitric oxide synthase (11) . Arginase I and arginase II are encoded by two distinct genes and hydrolyze l-Arg into urea and l-ornithine, the latter being the main substrate for the production of polyamines that are required for cell cycle progression. l-Arg is also metabolized by inducible nitric oxide synthase to citrulline and nitric oxide, a highly reactive compound important in vascular homeostasis, and as part of the cytotoxic mechanism of macrophages (12) . The importance of l-Arg on the immune response was initially suggested by the association between an impaired T-cell function and the reduction in serum l-Arg levels found in patients and rodents after liver transplantation or trauma, a process that was rapidly reversed by the enteral or parenteral supplementation of l-Arg (23) . However, the mechanisms ...
... pre-activated T cells that are focused on polyclonal proliferation already. This suppression was partly reversed by catalase addition (< 0·01) and generally reversed by addition of exogenous interleukin-2 (< 0·001) but had not been significantly decreased by nitric oxide synthase inhibition myeloperoxidase inhibition or addition of surplus arginine. Pursuing removal of PMNact suppressed T cells could react to additional arousal normally. Furthermore to suppressing proliferation co-culture with PMNact also induced a substantial reduction in T-cell viability that was reversed by catalase addition (< 0·05). The addition of the arginase inhibitor < 0·001). These data show that PMN when turned on can both induce T-cell loss of life and reversibly inhibit proliferation of turned on T cells. The systems underlying these distinctive processes and the consequences of arginase inhibitors on PMN induced ...
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Currie, G A.; Gyure, L; and Cifuentes, L, "Microenvironmental arginine depletion by macrophages in vivo." (1979). Subject Strain Bibliography 1979. 3434 ...
Top performende anti-Rind (Kuh) Arginase, Liver Antikörper für Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) vergleichen & kaufen.
Calithera Biosciences, Inc. is a clinical-stage pharmaceutical company. The Company focuses on discovering and developing small molecule drugs directed against tumor and immune cell targets that control key metabolic pathways in the tumor microenvironment. It is engaged in developing agents that take advantage of the metabolic requirements of tumor cells and cancer-fighting immune cells, such as cytotoxic T-cells. The Companys lead product candidate, CB-839, is a critical enzyme in tumor cells. Its other product candidate, CB-1158, is being developed for hematology and oncology indications. CB-1158 is a potent and selective orally bioavailable inhibitor of the enzyme arginase. CB-839 is a selective, reversible and orally bioavailable inhibitor of human glutaminase. CB-1158 has single agent anti-tumor activity in syngeneic mouse tumor models that has been demonstrated to act through an immune mechanism. CB-1158 is being tested in a Phase I clinical trial in patients with solid tumors.
In order to research the function of galectin-3 in tumor angiogenesis associated with tumor-associated macrophages (TAM) and tumor parenchyma, the galectin-3 expression was reconstituted in Tm1 melanoma cell line that lacks this protein. amounts, no significant distinctions between WTG3, WTN3, KOG3 or KON3 had been discovered (Fig. T6). BMDM from galectin-3 KO pets portrayed higher amounts of Arginase 1 but had been insensitive to its modulation by Meters2 prototypical cytokines Structured on the elevated reflection of Arginase I in WTG3 tumors, as well as on the idea that tumor-associated macrophages are polarized to the protumorigenic Meters2 phenotype, we CDP323 tested the impact of galectin-3 interruption in this sensation following. Once galectin-3 is normally viewed as a essential molecule in this polarizing event 9, we examined the behavior of BMDM from both WT and KO rodents after in vitro enjoyment with IL-4 (50?ng/mL) or TGF50?ng/mL pro-M1 stimuli with or without addition of exogenous ...
We be experiencing even shown that TFS can shut down eminence epilepticus (SE)--more than 30 min of constant possession activity without full revival of consciousness from seizures. In addition to complete nutrition, immunologic protection is transferred from mam to infant via chest tap and affectionateВ-infant bonding is promoted. Anti-inflammatory effects of Zocor in subjects with hypercholesteremia benicar 20 mg without a prescription arrhythmia types ecg. As a result, this debate will be limited to the bosses of emergent cardiac conditions that are more typically establish in children. The investigation of Leishmania arginase genes revealed the presence of undivided of the most visit glycosome import signals, the PST1 carboxyl signal, which consists of tree amino acids В- SKL (Opperdoes and Szikora 2006; da Silva et al. Every cardinal wants a whiter brighter smiling trusted plendil 10mg heart attack 50 years. The continued drink of these methods at an end many decades has also generated a ...
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Arginase 1 antibody (arginase, liver) for Conjugation, IM, WB. Anti-Arginase 1 pAb (GTX22111) is tested in Bovine samples. 100% Ab-Assurance.
Arginase 1 antibody (arginase, liver) for ICC/IF, IHC-P, WB. Anti-Arginase 1 pAb (GTX113131) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
enhanced arginase-1 activity was associated with increased lung inflammation. Moreover, in patients with pulmonary TB, lung damage correlated with increased serum activity of arginase-1, which was elevated in TB patients coinfected with helminths. Together, our data indicate that helminth coinfection induces arginase-1-expressing type 2 granulomas, thereby increasing inflammation and TB disease severity. These results also provide insight into the mechanisms by which helminth coinfections drive increased susceptibility, disease progression, and severity in TB.. ...
Argininemia is a rare, hereditary, urea cycle disorder. It is included in the category of inborn metabolic disorders of which there are no cures. It is also referenced in the literature as hyperargininemia, ARG1 deficiency, and arginase deficiency. This genetic disease is characterized by the absence of the enzyme, arginase. Arginase is the last enzyme necessary in the urea cycle, which is a metabolic pathway occurring in the liver. The metabolism of proteins in the diet creates nitrogen, as a waste product, in the circulatory system. The urea cycle processes this nitrogen, in the form of ammonia, from the blood, through a sequence of biochemical processes. Arginase is responsible for the incorporation of ammonia into urea, which can then be excreted from the body in urine. If urea cannot be synthesized, nitrogen accumulates in the blood and body tissues, as ammonia, which is a highly toxic substance, and may lead to brain damage and /or death. Arginase is also the enzyme necessary to regenerate ...
TY - JOUR. T1 - Arginine deprivation and tumour cell death: arginase and its inhibition. AU - Wheatley, Denis. AU - Philip, R.. AU - Campbell, E.. PY - 2003. Y1 - 2003. N2 - Arginase treatment of cell cultures reduced arginine in the medium to similar to micromolar levels within 5-30 min, and proved as effective as arginine-free medium (AFM) prepared by formulation. The enzyme was heat stable and as active at pH 7.2 as at pH 9.9. It persisted in culture for at least 3 days with only a small diminution in its speed of action, and still actively destroyed arginine after 6 days, since arginine supplementation failed to rescue viable cells.Addition of L-norvaline, an inhibitor of arginase, rescued cells from arginase-induced deprivation. Its efficacy at low concentrations was short-lived (probably , 1 day), while at higher concentrations it did not appear to inhibit completely the enzyme. However, L-norvaline at these same levels also slowed the growth of positive non-enzyme treated controls ...
Manganese in natural waters and earths crust - its availability to organisms; manganese transport in micro-organisms; manganese uptake and transport in plants; manganese metabolism in animals and humans including the toxicity of manganese; interrelations between manganese and other metal ions in health and disease; the use of manganese as a probe for elucidating the role of magnesium ions in ribozymes; Mn2+ as a probe of divalent metal ion binding and function in enzymes and other proteins; enzymes and proteins containing manganese - an overview; manganese (II) in concanavalin and other lectin proteins; manganese-activated phosphatases; manganese (II) as a probe for the mechanism and specificity of restriction endonucleases; the role of the binuclear manganese (II) site in xylose isomerase; arginase - a binuclear manganese metalloenzyme; the use of model complexes to elucidate the structure and function of manganese redox enzymes; manganese (II)-dependent extradiol-cleaving catechol ...
The IUPHAR/BPS Guide to Pharmacology. 2(S)-amino-6-boronohexanoic acid ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs.
DEHYDRO-2(S)-AMINO-6-BORONOHEXANOIC ACID | C6H13BNO5- | CID 657085 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
We can test the effectiveness of your compound against these parameters. We have previously demonstrated efficacy of bronchodilator drugs (β2 agonists, anticholinergics, Rho-kinase inhibitors) and anti-inflammatory drugs (glucocorticopsteroids, arginase inhibitors) in this model.. A unique feature of this model is that guinea pigs can be instrumented using a balloon catheter to measure lung function online in freely moving unrestrained animals. This way, not only hyperresponsiveness to histamine, but also the nature and size of the early and late asthmatic response can be measured including effects of pharmacological treatments hereon. This can be done without the need for surgical interventions during the treatment period, without the need for anaesthesia (which effectively blocks the neural component of airway hyperresponsiveness) and with the possibility of repeated and on-line measurements in time.. Furthermore, the guinea pig offers a number of unique characteristics that make the model ...
KW: skepticism about Stanfield Rogers; probably referring to: ON ARGINASE OF SHOPE PAPILLOMAS ORTH G, VIELLE F, CHANGEUX JP VIROLOGY 31: (4) 729-& 1967; my calendar: _Tue 16 Jan 1968~ Century 21- Human implications of biological discovery; jl 8/5/00 ...
NO synthesis is compromised during sepsis through lack of arginine de novo synthesis and may thereby contribute to impaired microcirculation and organ dysfunction. Supplementation of L-citrulline in septic patients will increase NO production without increased arginase activity and these effects will be studied on arginine-NO metabolism,improved organ function, vascular permeability and microcirculation ...
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In diesem Zusammenhang beschäftigen wir uns mit dem Stoffwechsel der Aminosäure Arginin in Tumorzellen und in Immunzellen. Seit unserer Erstbeschreibung der Expression des Arginin-verstoffwechselnden Enzyms Arginase in humanen Granulozyten zeigte es sich in den letzten Jahren, dass bestimmte myeloische Zellen (sogenannte myeloische Suppressorzellen, MDSC) in Tumorpatienten durch Arginase-vermittelte Depletion des Arginins die Funktion antitumoraler T-Lymphozyten hemmen. Auf der anderen Seite sind auch Tumorzellen von einer adäquaten Verfügbarkeit des Arginins abhängig und eine möglichst effiziente Arginin-Depletion stellt eine neue potentielle antitumorale Therapie dar. Wir entwickeln daher Strategien, um die beeinträchtigte Immunantwort im Kontext von Argininmangel wiederherzustellen (Projekt 1), studieren die Expression und Regulation von Arginin-Transportproteinen in Zellen des humanen Immunsystems (Projekt 2) sowie in Myelom-Tumorzellen (Projekt 3), etablieren und analysieren ...
Health, ...In a new study published in the scientific journal Circulation ... The fact that we could demonstrate the presence of arginase in severa...Complications in diabetes patients result from constrictions of the bl...In this present study the researchers analysed the function of the ar...,Enzyme,explains,angina,in,diabetics,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
A collection of disease information resources and questions answered by our Genetic and Rare Diseases Information Specialists for Arginase deficiency
The objective of the study was to explore the effects of galectin-9 on myeloid suppressor cells in Coxsackievirus B3 (CVB3)-induced myocarditis and the possible mechanisms involved. For this purpose, BALB/c male mice were infected with CVB3 on day 0 and then received intraperitoneal (IP) administration of recombinant galectin-9 or phosphate-buffered saline (PBS) daily from day 3 to day 7. The phenotypes and functions of myeloid suppressor cells were evaluated. The role and mechanism of myeloid suppressor cells and subsets in CVB3-induced myocarditis in vitro were explored. We found that galectin-9 remarkably increased the frequencies of CD11b+Gr-1+ cells in the cardiac tissue and spleen with myocarditis. Ly-6G+ cells were decreased and Ly-6C+ cells were increased in galectin-9-treated mice. In addition, CD11b+Gr-1+ cells were highly effective in suppressing CD4+ T cells. Moreover, our data demonstrate that CD11b+Gr-1+ cells are capable of expanding regulatory T cells (Tregs) from a preexisting
Symptoms of arginine deficiency include poor wound healing hair loss skin rash constipation Archangel monday 30th 2009f marchpm causes erectile dysfunction or users from the side effects of medicines high blood pressure or temporary psychological related ed. Ed Drugs Not Working L-arginine Metabolism For can Viagra Make You Go Deaf. Australias shark attack capital demands cull after teenager is mauled.. Instant Spandeck Instant Spandeck is a lightweight aluminium walkway idging and staging system in ONE versatile unit. The commonest cause of erectile dysfunction in young men is performance anxiety and the commonest cause of this is watching yourself trying to get or maintain I recon this is possibly the best natural impotence cure you can get. Mario Andretti greets fans during appearance in Rome.. A fanfic that combines the eponymous characters of Ed Edd n Eddy with the cast A description of tropes appearing in Fullmetal Eds. Budget Highlights 2016 erection commissioning or installation of ...
This is a pilot, open-label study of PEG-BCT-100 in patients with relapsed/refractory leukemia or lymphoma who have satisfied all inclusion/exclusion criteria.. Approximately 15 subjects will be enrolled or when 10 evaluable subjects are included in the study. Evaluable subjects are defined as subjects who have received 4 consecutive doses of PEG-BCT-100 1600U/kg within 6 weeks from the first 1600U/kg dose and completed the first disease response assessment.. After the initiation of trial treatment, safety parameters will be evaluated throughout the study. Adverse event (AE) will be graded according to the National Cancer Institute Common Toxicity Criteria for Adverse Events version 4.0 (NCI CTC AE v4). AE and serious AE (SAE) will be detected and recorded on the Case Report Forms (CRFs) until 15-28 days after the last dose of PEG-BCT-100.. Patients who achieve complete remission (CR)/complete remission with incomplete blood count recovery (CRi) following the 4 consecutive 1600U/kg doses may ...
It has become evident that tumor-induced immuno-suppressive elements in the growth microenvironment play a main part in suppressing normal features of effector Capital t cells. can promote antitumor results by re-establishing T-cell defenses (for review, discover ref. 6767).65, 68 1MT is anticipated to possess no serious side effects since it prevents IDO while sparing tryptophan dioxygenase, a hepatic enzyme that regulates body tryptophan amounts.69 Style and advancement of more effective IDO inhibitors is underway (for examine, discover ref. 60, 67, 70).60, 67, 70 Arginase and nitric-oxide synthase Change in the path involving the catabolism of L-arginine is linked to the reductions of T-cell expansion. Two essential digestive enzymes included in arginine rate of metabolism are arginase and inducible nitric oxide synthase (iNOS).9 Arginine is used by iNOS as a precursor for the production of nitric oxide (NO). Consequently, raised amounts of arginase and iNOS deplete arginine, an important ...
Product Name: AntipainDescription: Abbr: Alias: Source: Appearance: Pale yellow powderCAS NO: 1345808-25-4 Arginase inhibitor 1 Molecular Weight: 677.2Purity:
Expansion of myeloid-derived suppressor cells (MDSCs) has been documented in some murine models and patients with autoimmune diseases, but the exact role of MDSCs in this process remains largely unknown. The current study investigates this question in patients with systemic lupus erythematosus (SLE). Patients with active SLE showed a significant increase in HLA-DR−CD11b+CD33+ MDSCs, including both CD14+CD66b− monocytic and CD14−CD66b+ granulocytic MDSCs, in the peripheral blood compared to healthy controls (HCs). The frequency of MDSCs was positively correlated with the levels of serum arginase-1 (Arg-1) activity, T helper 17 (TH17) responses, and disease severity in SLE patients. Consistently, in comparison with MDSCs from HCs, MDSCs from SLE patients exhibited significantly elevated Arg-1 production and increased potential to promote TH17 differentiation in vitro in an Arg-1-dependent manner. Moreover, in a humanized SLE model, MDSCs were essential for the induction of TH17 responses and ...
In this study, we evaluated the nature of tumor-associated MDSC by comparing the phenotype and function of MDSC isolated from spleen and tumor sites from the same mice. It is known that MDSC can differentiate into MΦ and DC (Kusmartsev and Gabrilovich, 2003, 2005). Therefore, it was important to assure that we are indeed comparing cells with the same phenotype. We sorted MDSC based on the expression of Gr-1 and CD11b, two markers which are considered hallmarks of MDSC. MDSCs from the tumor site and spleen had similar morphology and phenotype. Expression of the macrophage cell marker F4/80 was slightly higher on tumor MDSC than on spleen cells. However, such rather minor phenotypic differences contrasted with profound differences in MDSC function. As was reported previously (Corzo et al., 2009), spleen MDSC contain a high level of ROS and a relatively modest level of NO and arginase I activity (although it was still elevated in comparison with Gr-1+CD11b+ cells from naive mice). In striking ...
The reticulocytes and the ageing red blood cells (RBCs) namely young (Y), middle-aged (M) and old RBCs (O) of female Wistar rats from different groups such as control animals (C), controls treated with vanadate (C + V), alloxan-induced diabetic (D), diabetic-treated with insulin (D + I) and vanadate (D + V), were fractionated on a percoll/BSA gradient. The following enzymes were measured-hexokinase (HK), glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R), glutathione-s-transferase (GST), alanine aminotransferase ΜlaAT), aspartate aminotransferase ΜsAT) and arginase in the hemolysates of all the RBCs fractions. Decreases in the activity of HK and AsAT by about 70%, arginase and GSH-Px by 30% in old RBCs were observed in comparison to reticulocytes of control animals. Increases in the activity of GSSG-R by 86%, AlaAT by more than 400% and GST by 70% were observed in old RBCs in comparison to reticulocytes of control animals.. Alloxan diabetic animals showed a further decrease in the ...
Arginine has only comparatively recently emerged as an intriguing amino acid in animal metabolism. Following the establishment of its pivotal role in the urea
In tumor-bearing mice and cancer patients, tumor progression is often associated with altered hematopoiesis leading to the accumulation of myeloid cells. Extensive studies in preclinical models indica
Thymosin α1 (Tα1) has been tested for cancer therapy for several years, in most cases, the anti-tumor effect of Tα1 was limited, especially when Tα1 was used as a single agent. The role of Tα1 in cancer treatment and the regulatory mechanisms by which Ta1 takes effects are not yet completely understood. Using a Lewis lung caner model, here we report that Tα1 used alone elevated CD8(+) T cells, but failed to inhibit tumor growth. Furthermore, immunosuppressive myeloid-derived suppressor cells (MDSCs) showed heightened Arginase 1 production in response to Tα1 treatment, which led to stronger suppression of anti-tumor immunity ...
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.
Prostate cancers develop immunosuppressive microenvironment resisting current treatments and the emerging immunotherapies. Hossain and colleagues identified a population of Lin−CD15HICD33LO granulocytic MDSCs (G-MDSCs) accumulating in patients during prostate cancer progression. The G-MDSCs secreted large amounts of Arginase 1, thereby suppressing T cells proliferation and activity. The MDSC functions were under control of the STAT3 transcription factor and a central immune checkpoint regulator. To functionally eliminate prostate cancer-associated G-MDSC, the authors successfully employed an siRNA-based approach to silence STAT3 specifically in TLR9-expressing G-MDSCs. These findings underscore the potential of oligonucleotide-based therapeutics to alleviate immunosuppressive signaling in advanced prostate cancers. ...
Arginase, Arginine Uptake and their Role in Leishmania (L.) amazonensis Physiology. Lucile Floeter-Winter, Universidade de São Paulo, Brazil ...
(1982) Thomasset et al. FEBS Letters. Cellular ornithine biosynthesis could be expected to play a significant role in putrescine formation and hence in growth. Two enzymes are involved in ornithine biosynthesis: arginase and transamidinase. These enzyme activities were studied in two human melano...
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Increased arginase activity contributes to airway nitric oxide (NO) deficiency in cystic fibrosis (CF). Whether down-stream products of arginase activity contribute to CF lung disease is currently unknown. The objective of this study was to test whether L-ornithine derived polyamines are present in CF airways and contribute to airway pathophysiology. Polyamine concentrations were measured in sputum of patients with CF and in healthy controls, using liquid chromatography-tandem mass spectrometry. The effect of spermine on airway smooth muscle mechanical properties was assessed in bronchial segments of murine airways, using a wire myograph. Sputum polyamine concentrations in stable CF patients were similar to healthy controls for putrescine and spermidine but significantly higher for spermine. Pulmonary exacerbations were associated with an increase in sputum and spermine levels. Treatment for pulmonary exacerbations resulted in decreases in arginase activity, L-ornithine and spermine concentrations in
Background Arginine depletion has shown promising anticancer effects among arginine auxotrophic cancers that are deficient in argininosuccinate synthetase (ASS) and/or ornithine transcarbamylase (OTC). Pegylated arginase (PEG-BCT-100 (rhArg1peg5000)) works as an arginine depletor by converting arginine to ornithine. However, accumulated ornithine can be channeled via ornithine decarboxylase (ODC) to produce polyamines that are known to promote tumor growth. We postulate that ODC inhibition enhances anticancer effects of BCT-100 in lung adenocarcinoma. Methods The activity of BCT-100 was tested in a panel of lung adenocarcinoma cell lines (H23, H358, HCC827, H1650, H1975, HCC2935 and HCC4006). Cell viability was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Protein expression was evaluated by Western Blot. Nude mice subcutaneous xenograft models (XMs) derived from these cell lines were used for in vivo study. An ODC inhibitor ...
Home » Type i IFN inhibits alternative macrophage activation during mycobacterium tuberculosis infection and leads to enhanced protection in the absence of IFN-γ ...
Abstract: Content of free ammonia and glutamine amide nitrogen was increased but content of protein amide nitrogen was decreased in brain tissue after bilateral nephrectomy of rats causing development an experimental uremia; an increased synthesis of urea and activation of arginase were observed in liver tissue. Flavonoids robinine and hyperine decreased the content of free ammonia, increased the content of glutamine amide nitrogen as well as of protein amide nitrogen in rat brain; robinine decreased slightly the urea synthesis and the arginase activity in liver slices of the nephrectomized ...
Secondary Amines Containing One Aromatic Nitro Group: Preparation, Nitrosation, Sustained Nitric Oxide Release, and the Synergistic Effects of Released Nitric Oxide and an Arginase Inhibitor on Vascular Smooth Muscle Cell Proliferation ...
Secondary Amines Containing One Aromatic Nitro Group: Preparation, Nitrosation, Sustained Nitric Oxide Release, and the Synergistic Effects of Released Nitric Oxide and an Arginase Inhibitor on Vascular Smooth Muscle Cell Proliferation ...
Journal of Allergy is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of allergy.
Background The leishmaniases certainly are a combined band of vector-borne parasitic illnesses that represent a significant international public medical condition; they participate in one of the most neglected tropical illnesses and have among the highest prices of morbidity and mortality. is normally a key aspect for parasite success. Here, we present that ageing decreases the expression degrees of arginase in macrophages, leading to better control of parasite development. Our results claim that age-related distinctions in the fat burning capacity of arginase in macrophages might donate to the bigger susceptibility of kids to leishmaniasis. Launch Attacks with protozoan parasites inflict an huge toll over the developing globe; they are significant reasons of mortality and morbidity and impede economic advancement. Leishmaniases are vector-borne illnesses, the parasites getting sent by bites of bloodstream feeding feminine sandflies and leading to different disease manifestations in human ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Arginine is derived from dietary protein intake, body protein breakdown, or endogenous de novo arginine production. The latter may be linked to the availability of citrulline, which is the immediate precursor of arginine and limiting factor for de novo arginine production. Arginine metabolism is highly compartmentalized due to the expression of the enzymes involved in arginine metabolism in various organs. A small fraction of arginine enters the NO synthase (NOS) pathway. Tetrahydrobiopterin (BH4) is an essential and rate-limiting cofactor for the production of NO ...
Tumor-associated macrophages (TAMs) and regulatory T cells (Tregs) are significant components of the microenvironment of solid tumors in the majority of cancers. TAMs sequentially develop from monocytes into functional macrophages. In each differentiation stage, TAMs obtain various immunosuppressive functions to maintain the tumor microenvironment (e.g., expression of immune checkpoint molecules, production of Treg-related chemokines and cytokines, production of arginase I). Although the main population of TAMs is immunosuppressive M2 macrophages, TAMs can be modulated into M1-type macrophages in each differential stage, leading to the suppression of tumor growth. Because the administration of certain drugs or stromal factors can stimulate TAMs to produce specific chemokines, leading to the recruitment of various tumor-infiltrating lymphocytes, TAMs can serve as targets for cancer immunotherapy. In this review, we discuss the differentiation, activation, and immunosuppressive function of TAMs, as well
Given the prevalence of inadequate nutrition in patients with pressure injuries, the use of high-protein oral nutrition supplements is common practice in the these patients. Additional arginine is needed during periods of stress and in the presence of injury. Therefore, arginine is a recommended component of oral nutritional supplements for patients with pressure injuries.
Possibly by assuring PETA that the only reason they were doing this was to save the animals from the summer heat, the authors of this paper exposed mice to the cold by sticking them into cages that had been pre-chilled to 4°. They saw that in BAT and WAT specifically, macrophages were activated in the alternative manner rather than the classical. Through gene expression analysis and flow cytometry, they saw that whereas the genes for alternative activation were progressively increased as the temperature was decreased, the genes for the classical activation were unchanged. Correspondingly, when Nguyen, et al. looked at mice that do not have the IL-4 and IL-13-and so, mice that did not have any choice but to activate macrophages through the classical pathway, they did not see the same increase in gene expression in these mice. They also made sure that this increase in alternative activation was specific to the BAT and WAT by looking for the same reactions in other tissues, including skeletal ...
Manganese is a component of metalloenzymes (an enzyme that contains a metal ion in its structure). Arginase a metalloenzyme in the liver responsible for creating urea, a component of urine. Glutamine synthetase a metalloenzyme is enzyme involved in the synthesis of glutamine. Phosphoenolpyruvate decarboxylase a metalloenzyme participates in the metabolism of blood sugar. Manganese-dependent superoxide dismutase found in mitochondria is an enzyme with antioxidant activity that protects tissues from the damaging effects of free radicals ...
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Wistar scientists have identified a marker that distinguishes PMN-MDSCs from neutrophils in the blood of patients with a variety of cancers.
Arginine supplementation has been shown to alleviate endothelial dysfunction and improve exercise performance through increasing nitric oxide production in patients with cardiopulmonary diseases. In addition, arginine supplementation could decrease accumulations of lactate and ammonia, metabolites involved in development of muscular fatigue. The aim of this study was to investigate the effect of short-term arginine supplementation on performance in intermittent anaerobic exercise and the underlying mechanism in well-trained male athletes. Ten elite male college judo athletes participated with a randomized crossover, placebo-controlled design. The subjects consumed 6 g/day arginine (ARG trial) or placebo (CON trial) for 3 days then performed an intermittent anaerobic exercise test on a cycle ergometer. Blood samples were collected before supplementation, before and during exercise and 0, 3, 6, 10, 30 and 60 min after exercise. ARG trial had significantly higher arginine concentrations than CON ...
TY - JOUR. T1 - Whole body arginine metabolism and nitric oxide synthesis in newborns with persistent pulmonary hypertension. AU - Castillo, Leticia. AU - De Rojas-Walker, T.. AU - Yu, Y. M.. AU - Sanchez, M.. AU - Chapman, T. E.. AU - Shannon, D.. AU - Tannenbaum, S.. AU - Burke, J. F.. AU - Young, V. R.. PY - 1995/1/1. Y1 - 1995/1/1. N2 - Despite the potential relevance of the L-arginine-nitric oxide (NO) pathway in the pathophysiology of pulmonary hypertension, no in vivo studies of the kinetics of arginine and NO have been conducted previously in this population. The terminal gua-nidino N-atom of L-arginine is the precursor for NO, which is oxidized to the stable inorganic nitrogen oxides, nitrite (NO2 −) and nitrate (NO3 −). Thus, synthesized NO is detected in serum or urine as NO2 − and NO3 −. The purpose of this investigation was to compare studies of whole body arginine metabolism twice in nine patients with persistent pulmonary hypertension of the newborn (PPHN), using a primed ...
Previously, we found increased expression of l-arginine metabolizing enzymes in both kidneys from two-kidney, one-clip (2K1C) hypertensive rats (Helle F, Hultstrom M, Skogstrand T, Palm F, Iversen BM. Am J Physiol Renal Physiol 296: F78-F86, 2009). In the present study, we investigate whether AT(1) receptor activation can induce the changes observed in 2K1C. Four groups of rats were infused with 80 ng/min ANG II or saline for 14 days and/or given 60 mg x kg(-1) x day(-1) losartan. Gene expression was studied in isolated preglomerular vessels by RT-PCR. Dose-responses to ANG II were studied in isolated preglomerular vessels with and without acute NOS inhibition [10(-4) mol/l N(G)-nitro-l-arginine methyl ester (l-NAME)]. Expressions of endothelial nitric oxide synthase (eNOS), caveolin-1, and arginase-2 were not changed by ANG II infusion. CAT1 (0.3 8 +/- 0.07 to 0.73 +/- 0.12, P , 0.05), CAT2 (1.14 +/- 0.29 to 2.74 +/- 0.48), DDAH2 (1.09 +/- 0.27 to 2.3 +/- 0.46), and arginase-1 (1.08 +/- 0.17 to ...
The catalase family of enzymes, which include a variety with a binuclear manganese active site, mitigate the risk from reactive oxygen species by facilitating the disproportionation of hydrogen peroxide into molecular oxygen and water. In this work, hydrogen peroxide disproportionation using complexes formed
article{8578976, abstract = {Solid tumors frequently coexist with a degree of local chronic inflammation. Recruited myeloid cells can therefore be considered as interesting vehicles for tumor-targeted delivery of therapeutic agents. Using in vivo imaging, the short-term accumulation of systemically injected monocytes, macrophages and myeloid-derived suppressor cells (MDSCs) was compared in mice bearing fat pad mammary carcinomas. Monocytes and macrophages demonstrated almost identical in vivo and ex vivo distribution patterns with maximal tumor-associated accumulation seen 48 hours after injection that remained stable over the 4-day follow-up period. However, a substantial accumulation of both cell types was also seen in the liver, spleen and lungs albeit decreasing over time in all three locations. The MDSCs exhibited a similar distribution pattern as the monocytes and macrophages, but demonstrated a better relative on-target fraction over time. Overall, our findings highlight off-target cell ...
Room 4137 Aloke Virmani Finn, MD Associate Professor of Medicine, University of Maryland Alternative Macrophages, Angiogenesis, and Plaque Progression in Atherosclerosis
The TLR4-active morphine metabolite morphine-3-glucuronide does not elicit macrophage classical activation in vitro. Khabbazi, Samira, Xie, Nan, Pu, Wenjun, Goumon, Yannick and Parat, Marie-Odile (2016) The TLR4-active morphine metabolite morphine-3-glucuronide does not elicit macrophage classical activation in vitro. Frontiers in Pharmacology,7 NOV: . doi:10.3389/fphar.2016.00441. https://www.ncbi.nlm.nih.gov/pubmed/27909407 Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in…
El Kasmi, K.C., J.E. Qualls, J.T. Pesce, A.M. Smith, R.W. Thompson, M. Henao-Tamayo, R.J. Basaraba, T. Konig, U. Schleicher, M.S. Koo, G. Kaplan, K.A. Fitzgerald, E.I. Tuomanen, I.M. Orme, T.D. Kanneganti, C. Bogdan, T.A. Wynn, and P.J. Murray. 2008. Toll-like receptor-induced arginase 1 in macrophages thwarts effective immunity against intracellular pathogens. Nat Immunol 9:1399-1406 ...
Fe2+-dependent enzyme. The enzyme is involved in the biosynthesis of the cyclic pentapeptide antibiotic viomycin. It differs from EC 1.14.11.34, 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming), because it does not form guanidine and (S)-1-pyrroline-5-carboxylate from 3-hydroxy-L-arginine ...
Supplies: Immunostain Technical Only Envelope (T693). Specimen Type: Tissue. Container/Tube: Immunostain Technical Only Envelope (T693). Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.. Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block. Additional Information:. 1. Information on accessing digital images of IHC stains and the manual requisition form can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/index.html. 2. Clients ordering stains using a manual requisition form will not have access to digital images.. 3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/faq.html. ...
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L-arginine, L-ornithine and L-glutamine levels. Tissue culture supernatants from CL-2, CL-19, and Renca cells were collected at 24, 48, and 72 hours. They were
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Looking for online definition of Liver-type aldolase in the Medical Dictionary? Liver-type aldolase explanation free. What is Liver-type aldolase? Meaning of Liver-type aldolase medical term. What does Liver-type aldolase mean?
The beneficial prognostic signification of surgical comprehensive- ness in retroperitoneal lymph node resection was observed in other series, too [35], and it isnt surprising that the likeli- hood of detecting lymph node metastasis increases substan- tially with the number of removed nodes [36] and assessment of all relevant regions [37]. NURSING DIAGNOSIS: Gamble in behalf of altered growth prototype (hazard factors: caregiver information deficit, earliest infant, hasty infant, or maladaptive feeding behaviors) Outcome Labelling and Estimation Infant will rally suitable vegetation and appropriate feeding behaviors: invariable increases in strain, dimension, and chair circumference; infant feeds aptly to age. com or telecommunicate them with questions or comments at [email protected] cheap 100mg viagra professional with mastercard erectile dysfunction treatment in bangladesh. After all, acknowledged the similarity of arginase to agmatinase, we conducted an evo- lutionary analysis of arginase and ...
The growth and metastasis of solid tumors not only depends on their ability to escape from immune surveillance but also hinges on their ability to invade the vasculature system as well as to induce the formation of new blood vessels. Gr-1(+)CD11b(+) myeloid-derived suppressor cells (MDSCs), overproduced in tumor-bearing hosts, contribute significantly to all these aspects. They also have a potential role in the osteolysis associated with bone metastases. They are formidable partners in tumor metastasis.
Monoclonal antibody (MAB) therapies targeting central mediators of inflammatory responses, such as TNFα, have become a common immunotherapy approach in contemporary clinical practice. Unfortunately, TNFα MAB therapy comes at a cost of significantly increased risk for the development of devastating infections (22-24, 34). Following our previous work, which established the central role of TNFα for the development of protective TH1 immunity in the relevant model of IFI (7, 25, 26, 35), here, we defined a previously unknown mechanism by which TNFα exerts its profound and lasting effect on host defenses. We provide evidence that (i) TNFα contributes to the generation of a uniquely stable DC1 phenotype, which is resistant to DC2 repolarization; (ii) TNFα-stabilized DCs arising during the afferent phase of the immune response are required for generation and sustenance of protective TH1/TH17 immunity; (iii) TNFα-mediated DC1 stabilization is associated with the H3K4me3 histone modification at ...
... arginase; several phosphatases and phosphoesterases-that includes two bridging carboxylate ligands and a bridging water or ...
In people with arginase deficiency, arginase is missing, and arginine is not broken down properly. consequently, urea cannot be ... "Arginase Deficiency". GeneReviews(®). University of Washington, Seattle. Retrieved 20 November 2016. update 2014 "Arginase ... Argininemia, also called arginase deficiency, is an autosomal recessive urea cycle disorder where a deficiency of the enzyme ... The ARG1 gene provides instructions for making an enzyme called arginase, this enzyme controls the last steps of the urea cycle ...
Waddington SN (2002). "Arginase in glomerulonephritis". Kidney Int. 61 (3): 876-81. doi:10.1046/j.1523-1755.2002.00236.x. PMID ...
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I ... Jiang M, Ding Y, Su Y, Hu X, Li J, Zhang Z (December 2006). "Arginase-flotillin interaction brings arginase to red blood cell ... "Androgen-regulated expression of arginase 1, arginase 2 and interleukin-8 in human prostate cancer". PloS One. 5 (8): e12107. ... Ishikawa T, Harada T, Koi H, Kubota T, Azuma H, Aso T (2007). "Identification of arginase in human placental villi". Placenta. ...
Arginase and urea synthesis". The Journal of Biological Chemistry. 184 (2): 479-484. PMID 15428427. Cohen, Stanley (1993). " ...
Arginase, type II is an arginase protein that in humans is encoded by the ARG2 gene. Arginase catalyzes the hydrolysis of ... "Molecular cloning of cDNA for nonhepatic mitochondrial arginase (arginase II) and comparison of its induction with nitric oxide ... "Species differences in expression pattern of arginase isoenzymes and differential effects of arginase inhibition on collagen ... Krause BJ, Prieto CP, Muñoz-Urrutia E, San Martín S, Sobrevia L, Casanello P (May 2012). "Role of arginase-2 and eNOS in the ...
Among the synthesized analogues, R = L-Leu possesses the most potent inhibitory activity (Ki = 9.1 nM). Arginase is a binuclear ... 2003). "Design of amino acid sulfonamides as transition-state analogue inhibitors of arginase". J Am Chem Soc. 125 (43): 13052- ... Maarsingh, Harm; Johan Zaagsma; Herman Meurs (October 2009). "Arginase: a key enzyme in the pathophysiology of allergic asthma ... "Probing the Specificity Determinants of Amino Acid Recognition by Arginase". Biochemistry. 48 (1): 121-131. doi:10.1021/ ...
2007). "Arginase-flotillin interaction brings arginase to red blood cell membrane". FEBS Lett. 580 (28-29): 6561-4. doi:10.1016 ...
4) Arginine is cleaved by arginase to form urea and ornithine. The ornithine is then transported back to the mitochondria to ... Deficiency of arginase) Hyperornithinemia, hyperammonemia, homocitrullinuria syndrome (Deficiency of the mitochondrial ... synthetase I OTC Ornithine transcarbamoylase ASS argininosuccinate synthetase ASL argininosuccinate lyase ARG1 arginase 1 ...
Arginase, which catalyses the conversion of arginine to urea and ornithine, is one of the five members of the urea cycle ... There are several arginase isozymes that differ in catalytic, molecular and immunological properties. Deficiency in the liver ... Baker BS, Tata JR, Xu Q (1993). "Developmental and hormonal regulation of the Xenopus liver-type arginase gene". Eur. J. ... The ureohydrolase superfamily includes arginase (EC 3.5.3.1), agmatinase (EC 3.5.3.11), formiminoglutamase (EC 3.5.3.8) and ...
They also promote extracellular matrix synthesis via production of ornithine, via arginase; this is used as a precursor for ...
Leopoldini M, Russo N, Toscano M (Aug 2009). "Determination of the catalytic pathway of a manganese arginase enzyme through ...
The Effect of Variation in Dietary Protein upon the Hepatic Arginase of the Rat". Biochemical Journal. 51 (5): 681-686. PMC ...
"Inhibition of S6K1 accounts partially for the anti-inflammatory effects of the arginase inhibitor L-norvaline". BMC ...
M2 macrophages activate arginase 1 (Arg1) that blocks iNOS activity and therefore inhibits nitric oxide production. They also ...
The best-known manganese-containing polypeptides may be arginase, the diphtheria toxin, and Mn-containing superoxide dismutase ...
L-Ornithine is one of the products of the action of the enzyme arginase on L-arginine, creating urea. Therefore, ornithine is a ...
The compound is found in legumes that contain canavanine, from which it is produced by the action of arginase. The most common- ...
Release of arginase, proline, polyaminases and TGF-β by the activated M2 cell is tied with wound repair and fibrosis. The ...
With his distinguished English pupil Henry Drysdale Dakin, Kossel investigated arginase, the ferment which hydrolyses arginine ...
They produce high levels of nitric oxide, but they almost do not have any arginase activity, so they can not produce urea. ... And unlike wound-healing macrophages, Mregs do not induct arginase, so they do not contribute to the production of the ...
... arginase 1, and 4-hydroxyphenyl-pyruvate dioxygenase" (PDF). Toxicological Sciences. 130 (2): 229-44. doi:10.1093/toxsci/kfs243 ...
In 1904, two Germans A. Kossel and H. D. Dakin had shown that arginine could be hydrolysed by the enzyme arginase to form ...
"Differential regulation of nitric oxide synthase-2 and arginase-1 by type 1/type 2 cytokines in vivo: granulomatous pathology ...
NO exerts antiproliferative effects by cGMP-dependent inhibiting Ca2+ influx or by directly inhibiting the activity of arginase ...
Arterial injury stimulates arginase activity and arginase I protein expression in the vessel wall, and local arginase ... In addition, this study shows that arginase promotes the entry of VSMCs into the cell cycle. Arginase inhibition or arginase I ... C, Effect of arginase I siRNA (Arg I) on arginase I protein expression VSMCs were treated with Arg I siRNA (100 nm) or non- ... Figure 4. Arginase expression and activity and in rat cultured VSMCs. A, Expression of arginase I protein in VSMCs. Data are ...
... of disease information resources and questions answered by our Genetic and Rare Diseases Information Specialists for Arginase ... or a blood test demonstrates reduced arginase enzyme. activity in the red blood cells. .[1][3]. In some cases, arginase ... associated with arginase deficiency. The parents of a person with arginase deficiency usually each carry one mutated copy of ... 3][2][1] Arginase deficiency is caused by mutations. in the ARG1 gene. and is inherited in an autosomal recessive. manner.[2][3 ...
The liver arginase was precipitated at 90%; whereas the kidney, brain, and GI arginase only precipitated at 50%. It was found ... Arginase activity is undetectable in amniotic fluid; however, fetal red cells have identical arginase properties to those of ... Arginase catalyzes L-arginine + H2O = L-ornithine + urea. This catalysis occurs within the liver, where arginase is cytoplasmic ... This genetic disease is characterized by the absence of the enzyme, arginase. Arginase is the last enzyme necessary in the urea ...
Piceatannol 3-O-glucoside 是 Rhubarb 的一种活性成分,通过抑制精氨酸酶 (Arginase) 活性激活内皮细胞一氧化氮合酶 (NO synthase),抑制 Arginase I 和 Arginase II,IC50 ... Arginase is present in 2 isoforms: arginase I, the hepatic isoform; and arginase II, the extrahepatic isoform; each of which is ... CB-1158 dihydrochloride (INCB01158 dihydrochloride) 是一种有效的,可口服的精氨酸酶 (arginase) 抑制剂,对重组人 arginase 1/2 的 IC50 值分别为 86 和 296 nM。 ...
Key Results Exposure to SIN-1 (25 μM, 24 h) or H 2O 2 (25 μM, 8 h) increased arginase I expression and arginase activity (35% ... Key Results Exposure to SIN-1 (25 μM, 24 h) or H 2O 2 (25 μM, 8 h) increased arginase I expression and arginase activity (35% ... Key Results Exposure to SIN-1 (25 μM, 24 h) or H 2O 2 (25 μM, 8 h) increased arginase I expression and arginase activity (35% ... Key Results Exposure to SIN-1 (25 μM, 24 h) or H 2O 2 (25 μM, 8 h) increased arginase I expression and arginase activity (35 ...
Many states test for arginase and citrin deficiencies. A handful also test for OTC and CPS1 deficiencies. ...
Arginase deficiency typically refers to decreased function of arginase I, the liver isoform of arginase. This deficiency is ... Arginase has been studied, in vitro, to treat several types of cancer, such as: breast, rectal, and colon. Arginase is used to ... Arginase belong to the ureohydrolase family of enzymes. Arginase catalyzes the fifth and final step in the urea cycle, a series ... Specifically, arginase converts L-arginine into L-ornithine and urea. Mammalian arginase is active as a trimer, but some ...
"Arginase. II. Distribution and properties of D-arginase". J. Biochem. 45: 1011-1020. D-arginase at the US National Library of ... D-arginase (EC 3.5.3.10) is an enzyme with systematic name D-arginine amidinohydrolase. This enzyme catalyses the following ... chemical reaction D-arginine + H2O ⇌ {\displaystyle \rightleftharpoons } D-ornithine + urea Arginase Nadai, Y. (1958). " ...
If untreated, arginase deficiency usually progresses to severe spasticity, loss of ambulation, complete loss of bowel and ... Arginase deficiency in untreated individuals is characterized by episodic hyperammonemia of variable degree that is ... Measurement of red blood cell arginase enzyme activity. Most affected individuals have no detectable arginase enzyme activity ( ... 2) Arginase enzyme activity is reduced in liver as well as red blood cells, but arginase enzyme activity in liver is rarely ...
Arginase deficiency is thought to be the least common of the urea cycle disorders. This entity also manifests itself in a ... encoded search term (Arginase%20Deficiency) and Arginase Deficiency What to Read Next on Medscape. Related Conditions and ... Prenatal diagnosis fro arginase deficiency by second-trimester fetal erythrocyte arginase assay and first-trimester ARG1 ... Arginase deficiency is thought to be the least common of the urea cycle disorders. This entity also manifests itself in a ...
... Renée C. Benson,1 Karen A. Hardy,1 and Claudia R. Morris2 ... Renée C. Benson, Karen A. Hardy, and Claudia R. Morris, "Arginase and Arginine Dysregulation in Asthma," Journal of Allergy, ...
Arginase deficiency is thought to be the least common of the urea cycle disorders. This entity also manifests itself in a ... encoded search term (Arginase Deficiency) and Arginase Deficiency What to Read Next on Medscape. Related Conditions and ... Prenatal diagnosis fro arginase deficiency by second-trimester fetal erythrocyte arginase assay and first-trimester ARG1 ... Arginase deficiency with lethal neonatal expression: evidence for the glutamine hypothesis of cerebral edema. J Pediatr. 2003 ...
... develop and commercialize Symbiosciences portfolio of arginase inhibitors, discovered as part of Mars cocoa flavanol... ... By inhibiting arginase, it may be possible to restore the tumor killing activity of cytotoxic T cells by preventing the ... Calitheras arginase program or other potential product candidates that Calithera develops may not progress through clinical ... About Tumor Immunology and Arginase Inhibitors. The field of tumor immunology is focused on developing agents that activate the ...
Arginase I is constitutive, and "extrahepatic" arginase (arginase II) is induced in vessel endothelium cells by ... Arginase and NOS compete for a common substratum-L-arginine. However, activity of arginase exceeds NOS activity by thousand ... The arginase is an enzyme of urea cycle that hydrolyzes L-arginine to ornithine and urea. There are two isoforms of this enzyme ... Arginase Inhibitor in the Pharmacological Correction of Endothelial Dysfunction. Mihail V. Pokrovskiy,1 Mihail V. Korokin,2 ...
... Mihail V. Pokrovskiy,1 Mihail V. Korokin,2 ... Felix Quitter, Hans R. Figulla, Markus Ferrari, John Pernow, and Christian Jung, "Increased arginase levels in heart failure ... Nikolay Avtandilyan, Hayarpi Javrushyan, Gayane Petrosyan, and Armen Trchounian, "The Involvement of Arginase and Nitric Oxide ... Waleed Barakat, Sherif El-Kannishy, Ahmad Fahmy, and Mohamed Askar, "Effectiveness of arginase inhibitors against ...
Arginase activity was associated with the cell-envelope fraction obtained by centrifugation of lysates. A Km of 22+/-3 mM was ... Divalent cations stimulated arginase activity, and the most potent activators were Co2+,Ni2+,Mn2+. The activity was highly ... In situ characterization of Helicobacter pylori arginase.. Mendz GL1, Holmes EM, Ferrero RL. ... Amino acid sequence analyses revealed important differences between the deduced structures of H. pylori arginase and those of ...
Rabbit polyclonal Liver Arginase antibody. Validated in WB, IP, IHC, ICC/IF and tested in Mouse, Rat. Cited in 22 publication(s ... Anti-liver Arginase antibody (ab91279). Rabbit polyclonal Liver Arginase antibody. Validated in WB, IP, IHC, ICC/IF and tested ... Liver Arginase was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to Liver Arginase and ... All lanes : Anti-liver Arginase antibody (ab91279) at 1 µg/ml. Lane 1 : Liver (Mouse) Tissue Lysate. Lane 2 : Liver (Rat) ...
Arginase and nitric oxide synthase (NOS) compete for the same substrate, L-arginine. The reciprocal regulation of arginase and ... Arginase levels are increased in patients with rheumatoid arthritis.. Huang LW1, Chang KL, Chen CJ, Liu HW. ... Arginase protein concentrations in supernatants of monocyte cultures from RA patients were also significantly higher than in ... The present study shows that both serum arginase activity and protein levels were significantly higher in patients with ...
Arginase-2, mitochondrial (Arg2), Arginase-1 (Arg1), Arginase (Arg2). This subpathway is part of the pathway urea cycle, which ... "Role of arginase 1 from myeloid cells in th2-dominated lung inflammation.". Barron L., Smith A.M., El Kasmi K.C., Qualls J.E., ... "Local arginase 1 activity is required for cutaneous wound healing.". Campbell L., Saville C.R., Murray P.J., Cruickshank S.M., ... arginase activity Source: MGI ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a direct assay for the function, process or ...
There are no specific protocols for Recombinant Human Liver Arginase protein (ab95487). Please download our general protocols ...
Conditional deletion of arginase 1 (Arg1) results in excessively prolonged and local inflammation. Images representing the ... Local arginase 1 activity is required for cutaneous wound healing.. Campbell L1, Saville CR1, Murray PJ2, Cruickshank SM3, ... Inhibition of arginase activity significantly delays cutaneous healing. (a) Representative hematoxylin and eosin-stained ... c) Arginase activity from isolated excisional wound tissue (measured through urea production) peaks at 5 days post wounding. (d ...
Arginase 1 Polyclonal Antibody from Invitrogen for Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry ... arginase; arginase 1 antibody; arginase 1, liver; arginase I; arginase, liver; Arginase-1; liver antibody; Liver-type arginase ... liver-type arginase antibody; Type I arginase; type I arginase antibody ... Cite Arginase 1 Polyclonal Antibody. The following antibody was used in this experiment: Arginase 1 Polyclonal Antibody from ...
B: the activity of arginase in the lungs from Wt and IL-13 Tg mice assessed by a commercial kit (n = 4 in each group, **P , ... IL-13 receptor α2-arginase 2 pathway mediates IL-13-induced pulmonary hypertension.. Cho WK1, Lee CM, Kang MJ, Huang Y, ... IL-13 induces the expression and activity of arginase (Arg) 1, Arg2, and nitric oxide synthase 3 (NOS3). A: the expression of ... IL-13 Tg mice spontaneously developed PH phenotype by the age of 2 mo with increased expression and activity of arginase 2 ( ...
enhanced arginase-1 activity was associated with increased lung inflammation. Moreover, in patients with pulmonary TB, lung ... infection resulted in accumulation of high arginase-1-expressing macrophages in the lung, which formed type 2 granulomas and ... Helminth-induced arginase-1 exacerbates lung inflammation and disease severity in tuberculosis. ... Helminth-induced arginase-1 exacerbates lung inflammation and disease severity in tuberculosis. ...
Arginase (D915_000092). This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.. View ... IPR014033 Arginase. IPR006035 Ureohydrolase. IPR023696 Ureohydrolase_dom_sf. IPR020855 Ureohydrolase_Mn_BS. ... IPR014033 Arginase. IPR006035 Ureohydrolase. IPR023696 Ureohydrolase_dom_sf. IPR020855 Ureohydrolase_Mn_BS. ... Belongs to the arginase family.PROSITE-ProRule annotation. Automatic assertion according to rulesi ...
  • The expression and function of arginase I in macrophages, hepatocytes, and vascular smooth muscle cells, is stimulated by lipopolysaccharide (LPS), IL-13, altered oxygen tension, and balloon dilatation of coronary arteries. (medchemexpress.cn)
  • Key Results Exposure to SIN-1 (25 μM, 24 h) or H 2 O 2 (25 μM, 8 h) increased arginase I expression and arginase activity (35% and 50%, respectively), which was prevented by ROCK inhibitor, Y-27632, PKC inhibitor, Gö6976 or siRNA to p115-Rho GEF. (elsevier.com)
  • combines with NO to form peroxynitrite (ONOO - ), further reducing NO. Oxidative species increase arginase activity, but the mechanism(s) involved are not known. (elsevier.com)
  • Our study determined the mechanism involved in peroxynitrite and hydrogen peroxide-induced enhancement in endothelial arginase activity. (elsevier.com)
  • We hypothesized that oxidative species increase arginase activity through PKC-activated RhoA/Rho kinase (ROCK) pathway. (elsevier.com)
  • Experimental approach Arginase activity/expression was analysed in bovine aortic endothelial cells (BAEC) treated with an ONOO - generator (SIN-1) or H 2 O 2 . (elsevier.com)
  • Conclusions and Implications Our data indicate that the oxidative species ONOO - and H 2 O 2 increase arginase activity/expression through PKC-mediated activation of RhoA/Rho kinase pathway. (elsevier.com)
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