Archaeoglobus fulgidus
Archaeoglobus
Archaeoglobales
RNA, Archaeal
Gene Expression Regulation, Archaeal
Nonheme Iron Proteins
Methanococcus
Hydrogensulfite Reductase
Archaea
One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.
Oxidoreductases Acting on Sulfur Group Donors
Crystallography, X-Ray
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
Models, Molecular
Enzyme Stability
Sequence Homology, Amino Acid
Crystallization
Sulfur
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Identification of the Archaeoglobus fulgidus endonuclease III DNA interaction surface using heteronuclear NMR methods. (1/193)
BACKGROUND: Endonuclease III is the prototype for a family of DNA-repair enzymes that recognize and remove damaged and mismatched bases from DNA via cleavage of the N-glycosidic bond. Crystal structures for endonuclease III, which removes damaged pyrimidines, and MutY, which removes mismatched adenines, show a highly conserved structure. Although there are several models for DNA binding by this family of enzymes, no experimental structures with bound DNA exist for any member of the family. RESULTS: Nuclear magnetic resonance (NMR) spectroscopy chemical-shift perturbation of backbone nuclei (1H, 15N, 13CO) has been used to map the DNA-binding site on Archaeoglobus fulgidus endonuclease III. The experimentally determined interaction surface includes five structural elements: the helix-hairpin-helix (HhH) motif, the iron-sulfur cluster loop (FCL) motif, the pseudo helix-hairpin-helix motif, the helix B-helix C loop, and helix H. The elements form a continuous surface that spans the active site of the enzyme. CONCLUSIONS: The enzyme-DNA interaction surface for endonuclease III contains five elements of the protein structure and suggests that DNA damage recognition may require several specific interactions between the enzyme and the DNA substrate. Because the target DNA used in this study contained a generic apurinic/apyrimidinic (AP) site, the binding interactions we observed for A. fulgidus endonuclease III should apply to all members of the endonuclease III family and several interactions could apply to the endonuclease III/AlkA (3-methyladenine DNA glycosylase) superfamily. (+info)Identification and characterization of a novel ferric reductase from the hyperthermophilic Archaeon Archaeoglobus fulgidus. (2/193)
Archaeoglobus fulgidus, a hyperthermophilic sulfate-reducing Archaeon, contains high Fe(3+)-EDTA reductase activity in its soluble protein fraction. The corresponding enzyme, which constitutes about 0.75% of the soluble protein, was purified 175-fold to homogeneity. Based on SDS-polyacrylamide gel electrophoresis, the ferric reductase consists of a single subunit with a M(r) of 18,000. The M(r) of the native enzyme was determined by size exclusion chromatography to be 40,000 suggesting that the native ferric reductase is a homodimer. The enzyme uses both NADH and NADPH as electron donors to reduce Fe(3+)-EDTA. Other Fe(3+) complexes and dichlorophenolindophenol serve as alternative electron acceptors, but uncomplexed Fe(3+) is not utilized. The purified enzyme strictly requires FMN or FAD as a catalytic intermediate for Fe(3+) reduction. Ferric reductase also reduces FMN and FAD, but not riboflavin, with NAD(P)H which classifies the enzyme as a NAD(P)H:flavin oxidoreductase. The enzyme exhibits a temperature optimum of 88 degrees C. When incubated at 85 degrees C, the enzyme activity half-life was 2 h. N-terminal sequence analysis of the purified ferric reductase resulted in the identification of the hypothetical gene, AF0830, of the A. fulgidus genomic sequence. The A. fulgidus ferric reductase shares amino acid sequence similarity with a family of NAD(P)H:FMN oxidoreductases but not with any ferric reductases suggesting that the A. fulgidus ferric reductase is a novel enzyme. (+info)The Archaeoglobus fulgidus D-lactate dehydrogenase is a Zn(2+) flavoprotein. (3/193)
Archaeoglobus fulgidus, a hyperthermophilic, archaeal sulfate reducer, is one of the few organisms that can utilize D-lactate as a sole source for both carbon and electrons. The A. fulgidus open reading frame, AF0394, which is predicted to encode a D-(-)-lactate dehydrogenase (Dld), was cloned, and its product was expressed in Escherichia coli as a fusion with the maltose binding protein (MBP). The 90-kDa MBP-Dld fusion protein was more efficiently expressed in E. coli when coexpressed with the E. coli dnaY gene, encoding the arginyl tRNA for the codons AGA and AGG. When cleaved from the fusion protein by treatment with factor Xa, the recombinant Dld (rDld) has an apparent molecular mass of 50 kDa, similar to that of the native A. fulgidus Dld enzyme. Both the purified MBP-Dld fusion protein and its rDld cleavage fragment have lactate dehydrogenase activities specific for D-lactate, are stable at 80 degrees C, and retain activity after exposure to oxygen. The flavin cofactor FAD, which binds rDld apoprotein with a 1:1 stoichiometry, is essential for activity. (+info)Cellulosome-like sequences in Archaeoglobus fulgidus: an enigmatic vestige of cohesin and dockerin domains. (4/193)
The distribution of cellulosomal cohesin domains among the sequences currently compiled in various sequence databases was investigated. Two cohesin domains were detected in two consecutive open reading frames (ORFs) of the recently sequenced genome of the archaeon Archaeoglobus fulgidus. Otherwise, no cohesin-like sequence could be detected in organisms other than those of the Eubacteria. One of the A. fulgidus cohesin-containing ORFs also harbored a dockerin domain, but the additional modular portions of both genes are undefined, both with respect to sequence homology and function. It is currently unclear what function(s) the putative cohesin and dockerin-containing proteins play in the life cycle of this organism. In particular, since A. fulgidus contains no known glycosyl hydrolase gene, the presence of a cellulosome can be excluded. The results suggest that cohesin and dockerin signature sequences cannot be used alone for the definitive identification of cellulosomes in genomes. (+info)Assembly of archaeal signal recognition particle from recombinant components. (5/193)
Signal recognition particle (SRP) takes part in protein targeting and secretion in all organisms. Searches for components of archaeal SRP in primary databases and completed genomes indicated that archaea possess only homologs of SRP RNA, and proteins SRP19 and SRP54. A recombinant SRP was assembled from cloned, expressed and purified components of the hyperthermophilic archaeon Archaeoglobus fulgidus. Recombinant Af-SRP54 associated with the signal peptide of bovine pre-prolactin translated in vitro. As in mammalian SRP, Af-SRP54 binding to Af-SRP RNA required protein Af-SRP19, although notable amounts bound in absence of Af-SRP19. Archaeoglobus fulgidus SRP proteins also bound to full-length SRP RNA of the archaeon Methanococcus jannaschii, to eukaryotic human SRP RNA, and to truncated versions which corresponded to the large domain of SRP. Dependence on SRP19 was most pronounced with components from the same species. Reconstitutions with heterologous components revealed a significant potential of human SRP proteins to bind to archaeal SRP RNAs. Surprisingly, M.jannaschii SRP RNA bound to human SRP54M quantitatively in the absence of SRP19. This is the first report of reconstitution of an archaeal SRP from recombinantly expressed purified components. The results highlight structural and functional conservation of SRP assembly between archaea and eucarya. (+info)Homology modeling and identification of serine 160 as nucleophile of the active site in a thermostable carboxylesterase from the archaeon Archaeoglobus fulgidus. (6/193)
The hyperthermophilic Archaeon Archaeoglobus fulgidus has a gene (AF1763) which encodes a thermostable carboxylesterase belonging to the hormone-sensitive lipase (HSL)-like group of the esterase/lipase family. Based on secondary structure predictions and a secondary structure-driven multiple sequence alignment with remote homologous proteins of known three-dimensional structure, we previously hypothesized for this enzyme the alpha/beta-hydrolase fold typical of several lipases and esterases and identified Ser160, Asp 255 and His285 as the putative members of the catalytic triad. In this paper we report the building of a 3D model for this enzyme based on the structure of the homologous brefeldin A esterase from Bacillus subtilis whose structure has been recently elucidated. The model reveals the topological organization of the fold corroborating our predictions. As regarding the active-site residues, Ser160, Asp255 and His285 are located close each other at hydrogen bond distances. The catalytic role of Ser160 as the nucleophilic member of the triad is demonstrated by the [(3)H]diisopropylphosphofluoridate (DFP) active-site labeling and sequencing of a radioactive peptide containing the signature sequence GDSAGG. (+info)Uracil-DNA glycosylase in the extreme thermophile Archaeoglobus fulgidus. (7/193)
Uracil-DNA glycosylase (UDG) is an essential enzyme for maintaining genomic integrity. Here we describe a UDG from the extreme thermophile Archaeoglobus fulgidus. The enzyme is a member of a new class of enzymes found in prokaryotes that is distinct from the UDG enzyme found in Escherichia coli, eukaryotes, and DNA-containing viruses. The A. fulgidus UDG is extremely thermostable, maintaining full activity after heating for 1.5 h at 95 degrees C. The protein is capable of removing uracil from double-stranded DNA containing either a U/A or U/G base pair as well as from single-stranded DNA. This enzyme is product-inhibited by both uracil and apurinic/apyrimidinic sites. The A. fulgidus UDG has a high degree of similarity at the primary amino acid sequence level to the enzyme found in Thermotoga maritima, a thermophilic eubacteria, and suggests a conserved mechanism of UDG-initiated base excision repair in archaea and thermophilic eubacteria. (+info)The Archean sulfur cycle and the early history of atmospheric oxygen. (8/193)
The isotope record of sedimentary sulfides can help resolve the history of oxygen accumulation into the atmosphere. We measured sulfur isotopic fractionation during microbial sulfate reduction up to 88 degrees C and show how sulfate reduction rate influences the preservation of biological fractionations in sediments. The sedimentary sulfur isotope record suggests low concentrations of seawater sulfate and atmospheric oxygen in the early Archean (3.4 to 2.8 billion years ago). The accumulation of oxygen and sulfate began later, in the early Proterozoic (2.5 to 0.54 billion years ago). (+info)
Archaeoglobus
The Archaeoglobus fulgidus genome is a circular chromosome roughly half the size of E. coli at 2,178,000 base pairs. Although ... PubMed references for Archaeoglobus PubMed Central references for Archaeoglobus Google Scholar references for Archaeoglobus ( ... for Archaeoglobus Search Species2000 page for Archaeoglobus MicrobeWiki page for Archaeoglobus LPSN page for Archaeoglobus ... Archaeoglobus is a genus of the phylum Euryarchaeota. Archaeoglobus can be found in high-temperature oil fields where they may ...
ARC3 family
Archaeoglobus fulgidus (370 aas; gbAE001071), Methanobacterium thermoautotrophicum (365 aas; gbAE000865) and Synechocystis (383 ...
Perchlorate
... and the archaeon Archaeoglobus fulgidus. With the exception of A. fulgidus, all known microbes that grow via perchlorate ...
Sulfite reductase
"Reaction cycle of the dissimilatory sulfite reductase from Archaeoglobus fulgidus". Biochemistry. 49 (41): 8912-21. doi:10.1021 ...
Dissimilatory sulfite reductase
"Reaction cycle of the dissimilatory sulfite reductase from Archaeoglobus fulgidus". Biochemistry. 49 (41): 8912-21. doi:10.1021 ...
List of sequenced archaeal genomes
November 1997). "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus". ... June 2010). "Complete genome sequence of Archaeoglobus profundus type strain (AV18)". Standards in Genomic Sciences. 2 (3): 327 ...
Archaellum
November 1997). "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus". ... Archaeoglobus fulgidus in 1977, and Pyrococcus horikoshii in 1998. Although genes of archaellins were identified all these ...
Cobalamin biosynthesis
... from Archaeoglobus Fulgidus". Journal of Structural and Functional Genomics. 7 (1): 37-50. doi:10.1007/s10969-006-9008-x. PMID ...
HgcG RNA
hgcG is significantly similar to a region of the Archaeoglobus fulgidus genome. The genes were named hgcA through hgcG ("high ...
NAD+ kinase
The structure of the NADK from the archaean Archaeoglobus fulgidus has been determined. In humans, the genes NADK and MNADK ... "Crystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP". Journal of Molecular ...
TRNA-intron endonuclease
The α′2 structure is only found in Archaeoglobus fulgidus and Thermoplasma acidophilum. The heterotetramer (αβ)2 structure is ...
F420H2DH family
The sulfate-reducing Archaeoglobus fulgidus (and several other archaea) also have this enzyme. Reduction of 2-hydroxyphenazine ...
Superoxide reductase
"Oxygen detoxification in the strict anaerobic archaeon Archaeoglobus fulgidus: superoxide scavenging by neelaredoxin". Mol. ...
Adenylyl-sulfate reductase
February 2002). "Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6-A resolution ...
Formylmethanofuran dehydrogenase
Formyl-MFR dehydrogenase was also isolated from Methanosarcina barkeri and Archaeoglobus fulgidus cell extracts. Molybdenum- ...
CRISPR
showed evidence that CRISPR repeat regions from the genome of Archaeoglobus fulgidus were transcribed into long RNA molecules ... May 2002). "Identification of 86 candidates for small non-messenger RNAs from the archaeon Archaeoglobus fulgidus". Proceedings ...
Radical SAM
Hyperthermophilic sulfate-reducing archaen Archaeoglobus fulgidus has been recently reported to enable anaerobic oxidation of ... Archaeoglobus fulgidus". The ISME Journal. 8 (11): 2153-66. doi:10.1038/ismej.2014.58. PMC 4992073. PMID 24763368. Benjdia A, ... PflD is reported to be responsible for the capacity of A. fulgidus to grow on a wide range of unsaturated carbons and fatty ...
Iron dependent repressor
Archaeoglobus fulgidus MDR1 (troR), a metal-dependent transcriptional repressor, which negatively regulates its own ...
Formylmethanofuran-tetrahydromethanopterin N-formyltransferase
Ftr from the mesophilic methanogen Methanosarcina barkeri and the sulphate-reducing archaeon Archaeoglobus fulgidus have a ...
Histone H2A
"The crystal structure of AF1521 a protein from Archaeoglobus fulgidus with homology to the non-histone domain of macroH2A". ...
Macro domain
"The crystal structure of AF1521 a protein from Archaeoglobus fulgidus with homology to the non-histone domain of macroH2A". J. ...
ATP-binding cassette transporter
... from Archaeoglobus fulgidus. Atomic-resolution structures of three other bacterial importers, E. coli BtuCD, E. coli maltose ...
Hyperthermophile
Archaeoglobus fulgidus Methanococcus jannaschii Aeropyrum pernix Sulfolobus Methanopyrus kandleri strain 116, an archaeon in 80 ...
UbiD protein domain
... with two members in Archaeoglobus fulgidus. They are related to UbiD, a 3-octaprenyl-4-hydroxybenzoate carboxy-lyase from ...
O-phospho-L-seryl-tRNA:Cys-tRNA synthase
Cys-tRNA synthase from Archaeoglobus fulgidus". Journal of Molecular Biology. 370 (1): 128-41. doi:10.1016/j.jmb.2007.04.050. ...
Coenzyme F420-0:L-glutamate ligase
... gamma-glutamyl ligase from Archaeoglobus fulgidus -- a member of a new family of non-ribosomal peptide synthases". Journal of ...
Coenzyme F420-1:gamma-L-glutamate ligase
... gamma-glutamyl ligase from Archaeoglobus fulgidus -- a member of a new family of non-ribosomal peptide synthases". Journal of ...
P-type ATPase
In the Archaeoglobus fulgidus CopA (TC# 3.A.3.5.7), invariant residues in helixes 6, 7 and 8 form two transmembrane metal ... The delivery of Cu+ by Archaeoglobus fulgidus Cu+-chaperone, CopZ (see TC# 3.A.3.5.7), to the corresponding Cu+-ATPase, CopA ( ... 2008) have determined structures of two constructs of the Cu (CopA) pump from Archaeoglobus fulgidus by cryoelectron microscopy ...
List of MeSH codes (B07)
... archaeoglobus MeSH B07.200.080.080.100 - archaeoglobus fulgidus MeSH B07.200.400.400 - halobacteriaceae MeSH B07.200.400.400. ...
O-phospho-L-serine-tRNA ligase
O-phospho-L-seryl-tRNACys In organisms like Archaeoglobus fulgidus, this enzyme ligates O-phosphoserine to tRNACys. Fukunaga R ...
Horizontal gene transfer in evolution
He describes the microorganism Archaeoglobus fulgidus as an anomaly with respect to a phylogenetic tree based upon the code for ...
Jürgen Brosius
Identification of 86 candidates for small non-messenger RNAs from the archaeon Archaeoglobus fulgidus. Proc Natl Acad Sci U S A ...
Signal recognition particle RNA
... from Bacillus subtilis Archaeal SRP RNA Archaeoglobus fulgidus Eukaryotic protist SRP RNA from Trypanosoma brucei Eukaryotic ...
Species Report for: Archaeoglobus fulgidus
Archaeoglobus fulgidus hypothetical protein af1134. arcfu-AF1563. Xaa-Pro-like_dom. Archaeoglobus fulgidus conserved ... Archaeoglobus fulgidus lysophospholipase. arcfu-AF1763. 6_AlphaBeta_hydrolase. Archaeoglobus fulgidus AF1763 gene, putative ... Archaeoglobus fulgidus hypothetical protein af0514. arcfu-AF0675. AlphaBeta_hydrolase. Archaeoglobus fulgidus 2-hydroxy-6- ... Archaeoglobus fulgidus AFEST AF1716 esterase. arcfu-o28594. Pectinacetylesterase-Notum. Archaeoglobus fulgidus VtpJ-therm, ...
Recombinant Archaeoglobus fulgidus Uncharacterized protein AF 0884 (AF 0884), E-Coli | Labstore
Exploring microbial growth of a model extremophile, archaeoglobus fulgidus, at elevated pressures
... Author. Oliver, Gina C. ... Archaeoglobus fulgidus (type strain VC16), was investigated up to 98 MPa in batch cultures for both chemoorganoheterotrophic ... Here, A. fulgidus was shown to grow, and in some cases also produce biofilm, over a range of elevated pressure conditions. To ... A. fulgidus is an anaerobic, hyperthermophilic sulfate reducing archaeon, first isolated from a shallow marine vent but has ...
RCSB PDB - 1VHV: Crystal structure of diphthine synthase
UFZ - Publication Index - Helmholtz-Centre for Environmental Research
Online Textbook and Video - Annenberg Learner
Molecules | Free Full-Text | Genetically Encodable Scaffolds for Optimizing Enzyme Function
The ferritin cage from the archaeon Archaeoglobus fulgidus (Figure 2) has been utilized to host an engineered GFP with 36 ... Crystal structures of a tetrahedral open pore ferritin from the hyperthermophilic archaeon archaeoglobus fulgidus. Structure ... Reprinted from Ban and co-workers [69]. (B) Assembly of A. fulgidus ferritin shells were engineered to be pH-responsive, ... Reprinted from Ban and co-workers [69]. (B) Assembly of A. fulgidus ferritin shells were engineered to be pH-responsive, ...
DeCS
Archaeoglobus fulgidus - Preferred Concept UI. M0029111. Scope note. A species of extremely thermophilic, sulfur-reducing ... Archaeoglobus fulgidus. Scope note:. Especie de archaea extremadamente termofílica y reductora de azufre. Crece a una ... Archaeoglobus fulgidus Descriptor Spanish: Archaeoglobus fulgidus Spanish from Spain Descriptor. ...
Pesquisa | BVS CLAP/SMR-OPAS/OMS
1.1.98.4: F420H2:quinone oxidoreductase - BRENDA Enzyme Database
NR all 88390.1
Satoshi Kimura, Ph.D. | Harvard Catalyst Profiles | Harvard Catalyst
Enzyme Encapsulation by a Ferritin Cage - Researcher | An App For Ac
Supplementary Table 1: List of the 93 genomes queried to build the manually curated 3D structure-guided multiple sequence...
Publications | NECAT
Identification of the gene responsible for the cblB complementation group of vitamin B12-dependent methylmalonic aciduria -...
Publications | Istvan Botos, Ph.D. | NIDDK
TREE NUMBER DESCRIPTOR
Archaea Volume 2021; 2021 - PMC
MeSH Browser
Archaeoglobus fulgidus Preferred Term Term UI T058337. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1998). ... Archaeoglobus fulgidus Preferred Concept UI. M0029111. Registry Number. txid2234. Scope Note. A species of extremely ... Archaeoglobus fulgidus. Tree Number(s). B02.200.080.080.100. Unique ID. D019611. RDF Unique Identifier. http://id.nlm.nih.gov/ ...
MeSH Browser
Archaeoglobus fulgidus Preferred Term Term UI T058337. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1998). ... Archaeoglobus fulgidus Preferred Concept UI. M0029111. Registry Number. txid2234. Scope Note. A species of extremely ... Archaeoglobus fulgidus. Tree Number(s). B02.200.080.080.100. Unique ID. D019611. RDF Unique Identifier. http://id.nlm.nih.gov/ ...
Code System Concept
afu00252 | Interagency Modeling and Analysis Group
µ
Thermophilic Ferritin | NIH 3D Print Exchange
Alexander Wlodawer, Ph.D. | Principal Investigators | NIH Intramural Research Program
MESH TREE NUMBER CHANGES - 2010 MeSH. August 28, 2009
Mechanism of action of organophosphorus and carbamate insecticides. | Environmental Health Perspectives | Vol. 87, No.
Ferritin2
- Positively supercharged green fluorescent protein is efficiently taken up by Archaeoglobus fulgidus ferritin in a tunable fashion. (researcher-app.com)
- The caged bird sings: The natural ferritin nanocage from Archaeoglobus fulgidus can encapsulate positively charged proteins (green) within its lumenal cavity through electrostatic interactions. (researcher-app.com)
22341
- 2336 / Ther.mari / Thermotoga maritima 2039 / Trop.whip / Tropheryma whipplei 670 / Vibr.para / Vibrio parahaemolyticus archae 56636 / Aero.pern / Aeropyrum pernix 2234 / Arch.fulg / Archaeoglobus fulgidus 2243 / Halo.sp. (misynpat.org)
Species1
- A. fulgidus' metabolic plasticity and capacity for biofilm production reflects adaptive mechanisms that lend insight into how this species thrives in extreme and fluctuating environments. (rpi.edu)
Growth3
- To explore how elevated pressures affect the metabolism and physiology of deep-sea and subsurface microorganisms, growth of a model extremophile, Archaeoglobus fulgidus (type strain VC16), was investigated up to 98 MPa in batch cultures for both chemoorganoheterotrophic and chemolithoautotrophic metabolisms. (rpi.edu)
- In autotrophic HHP conditions, A. fulgidus displayed piezotolerance with similar growth rates and maximum cell densities observed at up to 40 MPa and little to no growth was observed at 60 MPa. (rpi.edu)
- A. fulgidus biofilm production was observed in certain heterotrophic conditions from 0.1-50 MPa under HHP batch cultivation conditions due to both low calcium concentrations in the growth medium and the presence of a stainless steel needle that created a nucleation site. (rpi.edu)
Type1
- We have solved the structures of the proteolytic domain of A and B type Lon proteases, encoded by E. coli and Archaeoglobus fulgidus, as well as the N-terminal and α domains of E. coli Lon. (nih.gov)