Arachidonate Lipoxygenases: Enzymes catalyzing the oxidation of arachidonic acid to hydroperoxyarachidonates. These products are then rapidly converted by a peroxidase to hydroxyeicosatetraenoic acids. The positional specificity of the enzyme reaction varies from tissue to tissue. The final lipoxygenase pathway leads to the leukotrienes. EC 1.13.11.- .Lipoxygenases: Dioxygenases that catalyze the peroxidation of methylene-interrupted UNSATURATED FATTY ACIDS.Lipoxygenase: An enzyme of the oxidoreductase class primarily found in PLANTS. It catalyzes reactions between linoleate and other fatty acids and oxygen to form hydroperoxy-fatty acid derivatives.Arachidonate 12-Lipoxygenase: An enzyme that catalyzes the oxidation of arachidonic acid to yield 12-hydroperoxyarachidonate (12-HPETE) which is itself rapidly converted by a peroxidase to 12-hydroxy-5,8,10,14-eicosatetraenoate (12-HETE). The 12-hydroperoxides are preferentially formed in PLATELETS.Arachidonate 15-Lipoxygenase: An enzyme that catalyzes the oxidation of arachidonic acid to yield 15-hydroperoxyarachidonate (15-HPETE) which is rapidly converted to 15-hydroxy-5,8,11,13-eicosatetraenoate (15-HETE). The 15-hydroperoxides are preferentially formed in NEUTROPHILS and LYMPHOCYTES.Arachidonic Acid: An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.Arachidonate 5-Lipoxygenase: An enzyme that catalyzes the oxidation of arachidonic acid to yield 5-hydroperoxyarachidonate (5-HPETE) which is rapidly converted by a peroxidase to 5-hydroxy-6,8,11,14-eicosatetraenoate (5-HETE). The 5-hydroperoxides are preferentially formed in leukocytes.Arachidonic AcidsLipoxygenase Inhibitors: Compounds that bind to and inhibit that enzymatic activity of LIPOXYGENASES. Included under this category are inhibitors that are specific for lipoxygenase subtypes and act to reduce the production of LEUKOTRIENES.12-Hydroxy-5,8,10,14-eicosatetraenoic Acid: A lipoxygenase metabolite of ARACHIDONIC ACID. It is a highly selective ligand used to label mu-opioid receptors in both membranes and tissue sections. The 12-S-HETE analog has been reported to augment tumor cell metastatic potential through activation of protein kinase C. (J Pharmacol Exp Ther 1995; 274(3):1545-51; J Natl Cancer Inst 1994; 86(15):1145-51)Linoleic Acids: Eighteen-carbon essential fatty acids that contain two double bonds.Masoprocol: A potent lipoxygenase inhibitor that interferes with arachidonic acid metabolism. The compound also inhibits formyltetrahydrofolate synthetase, carboxylesterase, and cyclooxygenase to a lesser extent. It also serves as an antioxidant in fats and oils.Lipid Peroxides: Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.Leukotrienes: A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.Hydroxyeicosatetraenoic Acids: Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes.Soybeans: An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.Eicosanoids: A class of compounds named after and generally derived from C20 fatty acids (EICOSANOIC ACIDS) that includes PROSTAGLANDINS; LEUKOTRIENES; THROMBOXANES, and HYDROXYEICOSATETRAENOIC ACIDS. They have hormone-like effects mediated by specialized receptors (RECEPTORS, EICOSANOID).Linoleic Acid: A doubly unsaturated fatty acid, occurring widely in plant glycosides. It is an essential fatty acid in mammalian nutrition and is used in the biosynthesis of prostaglandins and cell membranes. (From Stedman, 26th ed)5,8,11,14-Eicosatetraynoic Acid: A 20-carbon unsaturated fatty acid containing 4 alkyne bonds. It inhibits the enzymatic conversion of arachidonic acid to prostaglandins E(2) and F(2a).Prostaglandin-Endoperoxide Synthases: Enzyme complexes that catalyze the formation of PROSTAGLANDINS from the appropriate unsaturated FATTY ACIDS, molecular OXYGEN, and a reduced acceptor.Fatty Acids, Unsaturated: FATTY ACIDS in which the carbon chain contains one or more double or triple carbon-carbon bonds.Corylus: A plant genus of the family BETULACEAE known for the edible nuts.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine: A dual inhibitor of both cyclooxygenase and lipoxygenase pathways. It exerts an anti-inflammatory effect by inhibiting the formation of prostaglandins and leukotrienes. The drug also enhances pulmonary hypoxic vasoconstriction and has a protective effect after myocardial ischemia.Lipoxins: Trihydroxy derivatives of eicosanoic acids. They are primarily derived from arachidonic acid, however eicosapentaenoic acid derivatives also exist. Many of them are naturally occurring mediators of immune regulation.Phospholipases A: Phospholipases that hydrolyze one of the acyl groups of phosphoglycerides or glycerophosphatidates.Eicosanoic Acids: 20-carbon saturated monocarboxylic acids.Blood Platelets: Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.Phospholipases A2: Phospholipases that hydrolyze the acyl group attached to the 2-position of PHOSPHOGLYCERIDES.Fatty Acids: Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)Calcimycin: An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.Cyclooxygenase Inhibitors: Compounds or agents that combine with cyclooxygenase (PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES) and thereby prevent its substrate-enzyme combination with arachidonic acid and the formation of eicosanoids, prostaglandins, and thromboxanes.Solanum tuberosum: A plant species of the genus SOLANUM, family SOLANACEAE. The starchy roots are used as food. SOLANINE is found in green parts.Kinetics: The rate dynamics in chemical or physical systems.Linolenic Acids: Eighteen-carbon essential fatty acids that contain three double bonds.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Gas Chromatography-Mass Spectrometry: A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Reticulocytes: Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.Prostaglandins E: (11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.Cyclooxygenase 1: A constitutively-expressed subtype of prostaglandin-endoperoxide synthase. It plays an important role in many cellular processes.Thromboxane B2: A stable, physiologically active compound formed in vivo from the prostaglandin endoperoxides. It is important in the platelet-release reaction (release of ADP and serotonin).Phospholipids: Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Indomethacin: A non-steroidal anti-inflammatory agent (NSAID) that inhibits the enzyme cyclooxygenase necessary for the formation of prostaglandins and other autacoids. It also inhibits the motility of polymorphonuclear leukocytes.Prostaglandins: A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.Leukotriene B4: The major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990)Oxylipins: Eighteen-carbon cyclopentyl polyunsaturated fatty acids derived from ALPHA-LINOLENIC ACID via an oxidative pathway analogous to the EICOSANOIDS in animals. Biosynthesis is inhibited by SALICYLATES. A key member, jasmonic acid of PLANTS, plays a similar role to ARACHIDONIC ACID in animals.Fatty Acids, Essential: Long chain organic acid molecules that must be obtained from the diet. Examples are LINOLEIC ACIDS and LINOLENIC ACIDS.Phospholipases: A class of enzymes that catalyze the hydrolysis of phosphoglycerides or glycerophosphatidates. EC 3.1.-.

Leukotriene A synthase activity of purified mouse skin arachidonate 8-lipoxygenase expressed in Escherichia coli. (1/145)

Mouse skin 8-lipoxygenase was expressed in COS-7 cells by transient transfection of its cDNA in pEF-BOS carrying an elongation factor-1alpha promoter. When crude extract of the transfected COS-7 cells was incubated with arachidonic acid, 8-hydroxy-5,9,11, 14-eicosatetraenoic acid was produced as assessed by reverse- and straight-phase high performance liquid chromatographies. The recombinant enzyme also reacted on alpha-linolenic and docosahexaenoic acids at almost the same rate as that with arachidonic acid. Eicosapentaenoic and gamma-linolenic acids were also oxygenated at 43% and 56% reaction rates of arachidonic acid, respectively. In contrast, linoleic acid was a poor substrate for this enzyme. The 8-lipoxygenase reaction with these fatty acids proceeded almost linearly for 40 min. The 8-lipoxygenase was also expressed in an Escherichia coli system using pQE-32 carrying six histidine residues at N-terminal of the enzyme. The expressed enzyme was purified over 380-fold giving a specific activity of approximately 0.2 micromol/45 min per mg protein by nickel-nitrilotriacetate affinity chromatography. The enzymatic properties of the purified 8-lipoxygenase were essentially the same as those of the enzyme expressed in COS-7 cells. When the purified 8-lipoxygenase was incubated with 5-hydroperoxy-6,8,11, 14-eicosatetraenoic acid, two epimers of 6-trans-leukotriene B4, degradation products of unstable leukotriene A4, were observed upon high performance liquid chromatography. Thus, the 8-lipoxygenase catalyzed synthesis of leukotriene A4 from 5-hydroperoxy fatty acid. Reaction rate of the leukotriene A synthase was approximately 7% of arachidonate 8-lipoxygenation. In contrast to the linear time course of 8-lipoxygenase reaction with arachidonic acid, leukotriene A synthase activity leveled off within 10 min, indicating suicide inactivation.  (+info)

Identification of amino acid determinants of the positional specificity of mouse 8S-lipoxygenase and human 15S-lipoxygenase-2. (2/145)

Phorbol ester-inducible mouse 8S-lipoxygenase (8-LOX) and its human homologue, 15S-lipoxygenase-2 (15-LOX-2), share 78% identity in amino acid sequences, yet there is no overlap in their positional specificities. In this study, we investigated the determinants of positional specificity using a random chimeragenesis approach in combination with site-directed mutagenesis. Exchange of the C-terminal one-third of the 8-LOX with the corresponding portion of 15-LOX-2 yielded a chimeric enzyme with exclusively 15S-lipoxygenase activity. The critical region was narrowed down to a cluster of five amino acids by expression of multiple cDNAs obtained by in situ chimeragenesis in Escherichia coli. Finally, a pair of amino acids, Tyr(603) and His(604), was identified as the positional determinant by site-directed mutagenesis. Mutation of both of these amino acids to the corresponding amino acids in 15-LOX-2 (Asp and Val, respectively) converted the positional specificity from 8S to 90% 15S without yielding any other by-products. Mutation of the corresponding residues in 15-LOX-2 to the 8-LOX sequence changed specificity to 50% oxygenation at C-8 for one amino acid substitution and 70% at C-8 for the double mutant. Based on the crystal structure of the reticulocyte 15-LOX, these two amino acids lie opposite the open coordination position of the catalytic iron in a likely site for substrate binding. The change from 8 to 15 specificity entails a switch in the head to tail binding of substrate. Enzymes that react with substrate "head first" (5-LOX and 8-LOX) have a bulky aromatic amino acid and a histidine in these positions, whereas lipoxygenases that accept substrates "tail first" (12-LOX and 15-LOX) have an aliphatic residue with a glutamine or aspartate. Thus, this positional determinant of the 8-LOX and 15-LOX-2 may have significance for other lipoxygenases.  (+info)

8S-lipoxygenase products activate peroxisome proliferator-activated receptor alpha and induce differentiation in murine keratinocytes. (3/145)

To determine the function and mechanism of action of the 8S-lipoxygenase (8-LOX) product of arachidonic acid, 8S-hydroxyeicosatetraenoic acid (8S-HETE), which is normally synthesized only after irritation of the epidermis, transgenic mice with 8-LOX targeted to keratinocytes through the use of a loricrin promoter were generated. Histological analyses showed that the skin, tongue, and stomach of transgenic mice are highly differentiated, and immunoblotting and immunohistochemistries of skin showed higher levels of keratin-1 expression compared with wild-type mice. The labeling index, however, of the transgenic epidermis was twice that of the wild-type epidermis. Furthermore, 8S-HETE treatment of wild-type primary keratinocytes induced keratin-1 expression. Peroxisome proliferator activated receptor alpha (PPARalpha) was identified as a crucial component of keratin-1 induction through transient transfection with expression vectors for PPARalpha, PPARgamma, and a dominant-negative PPAR, as well as through the use of known PPAR agonists. From these studies, it is concluded that 8S-HETE plays an important role in keratinocyte differentiation and that at least some of its effects are mediated by PPARalpha.  (+info)

A pertussis toxin-sensitive 8-lipoxygenase pathway is activated by a nicotinic acetylcholine receptor in aplysia neurons. (4/145)

Acetylcholine (ACh) activates two types of chloride conductances in Aplysia neurons that can be distinguished by their kinetics and pharmacology. One is a rapidly desensitizing current that is blocked by alpha-conotoxin-ImI and the other is a sustained current that is insensitive to the toxin. These currents are differentially expressed in Aplysia neurons. We report here that neurons that respond to ACh with a sustained chloride conductance also generate 8-lipoxygenase metabolites. The sustained chloride conductance and the activation of 8-lipoxygenase have similar pharmacological profiles. Both are stimulated by suberyldicholine and nicotine, and both are inhibited by alpha-bungarotoxin. Like the sustained chloride conductance, the activation of 8-lipoxygenase is not blocked by alpha-conotoxin-ImI. In spite of the similarities between the metabolic and electrophysiological responses, the generation of 8-lipoxygenase metabolites does not appear to depend on the ion current since an influx of chloride ions is neither necessary nor sufficient for the formation of the lipid metabolites. In addition, the application of pertussis toxin blocked the ACh-activated release of arachidonic acid and the subsequent production of 8-lipoxygenase metabolites, yet the ACh-induced activation of the chloride conductance is not dependent on a G protein. Our results are consistent with the idea that the nicotinic ACh receptor that activates the sustained chloride conductance can, independent of the chloride ion influx, initiate lipid messenger synthesis.  (+info)

Elevated expression of 12/15-lipoxygenase and cyclooxygenase-2 in a transgenic mouse model of prostate carcinoma. (5/145)

Changes in expression of arachidonic acid (AA) metabolizing enzymes are implicated in the development and progression of human prostate carcinoma (Pca). Transgenic mouse models of Pca that progress from high-grade prostatic intraepithelial neoplasia (HGPIN) to invasive and metastatic carcinoma could facilitate study of the regulation and function of these genes in Pca progression. Herein we characterize the AA-metabolizing enzymes in transgenic mice established with a prostate epithelial-specific long probasin promoter and the SV40 large T antigen (LPB-Tag mice) that develop extensive HGPIN and invasive and metastatic carcinoma with neuroendocrine (NE) differentiation. Murine 8-lipoxygenase (8-LOX), homologue of the 15-LOX-2 enzyme that is expressed in benign human prostatic epithelium and reduced in Pca, was not detected in wild-type or LPB-Tag prostates as determined by enzyme assay, reverse transcription-PCR, and immunohistochemistry. The most prominent AA metabolite in mouse prostate was 12-HETE. Wild-type prostate (dorsolateral lobe) converted 1.6 +/- 0.5% [(14)C]AA to 12-HETE (n = 7), and this increased to 8.0 +/- 4.4% conversion in LPB-Tag mice with HGPIN (n = 13). Quantitative real-time reverse transcription-PCR and immunostaining correlated the increased 12-HETE synthesis with increased neoplastic epithelial expression of 12/15-LOX, the leukocyte-type (L) of 12-LOX and the murine homologue of human 15-LOX-1. Immunostaining showed increased L12-LOX in invasive carcinoma and approximately one-half of metastatic foci. COX-2 mRNA was detectable in neoplastic prostates with HGPIN but not in wild-type prostate. By immunostaining, COX-2 was increased in the neoplastic epithelium of HGPIN but was absent in foci of invasion and metastases. We conclude that (a) AA metabolism in wild-type mouse prostate differs from humans in the basal expression of LOXs (15-LOX-2 in human, absence of its 8-LOX homologue in mouse prostate); (b) increased expression of 12/15-LOX in HGPIN and invasive carcinoma of the LPB-Tag model is similar to the increased 15-LOX-1 in high-grade human Pca; and (c) the LPB-Tag model shows increased COX-2 in HGPIN, and therefore, it may allow additional definition of the role of this enzyme in the subset of human HGPINs or other precursor lesions that are COX-2 positive, as well as investigation of its contribution to neoplastic cell proliferation and tumor angiogenesis in Pca.  (+info)

Upregulation of 8-lipoxygenase in the dermatitis of IkappaB-alpha-deficient mice. (6/145)

Neonatal mice deficient in IkappaB-alpha, an inhibitor of the ubiquitous transcription factor NF-kappaB, develop severe and widespread dermatitis shortly after birth. In humans, inflammatory skin disorders such as psoriasis are associated with accumulation in the skin of the unusual arachidonic acid metabolite 12R-hydroxyeicosatetraenoic acid (12R-HETE), a product of the enzyme 12R-lipoxygenase. To examine the etiology of the murine IkappaB-alpha-deficient skin phenotype, we investigated the expression of lipoxygenases and the metabolism of exogenous arachidonic acid in the skin. In the IkappaB-alpha-deficient animals, the major lipoxygenase metabolite was 8S-HETE, formed together with a minor amount of 12S-HETE; 12R-HETE synthesis was undetectable. Skin from the wild-type littermates formed 12S-HETE as the almost exclusive lipoxygenase metabolite. Upregulation of 8S-lipoxygenase (8-LOX) in IkappaB-alpha-deficient mice was confirmed at the transcriptional and translational level using ribonuclease protection assay and western analysis. In immunohistochemical studies, increased expression of 8-LOX was detected in the stratum granulosum of the epidermis. In the stratum granulosum, 8-LOX may be involved in the terminal differentiation of keratinocytes. Although mouse 8S-lipoxygenase and human 12R-lipoxygenase are not ortholog genes, we speculate that in mouse and humans the two different enzymes may fulfill equivalent functions in the progression of inflammatory dermatoses.  (+info)

Arachidonate lipoxygenase (ALOX) and cyclooxygenase (COX) polymorphisms and colon cancer risk. (7/145)

In the human colon, arachidonic acid is metabolized primarily by cyclooxygenase (COX) and arachidonate lipoxygenase (ALOX) to bioactive lipids, which are implicated in colon cancer risk. Several polymorphisms in ALOX and COX genes have been identified, including G-1752A, G-1699A and Glu254Lys in ALOX5; Gln261Arg in ALOX12; Leu237Met and Val481Ile in COX1; and C-645T and Val511Ala in COX2. Because of the significant role of arachidonic acid metabolism in colon cancer, we hypothesized that these polymorphisms could influence susceptibility to colon cancer. We addressed this hypothesis in African-Americans and Caucasians using colon cancer cases (n = 293) and hospital- (n = 229) and population-based (n = 304) control groups. Polymorphisms did not differ between the control groups (P > 0.05); thus, they are combined for all analyses presented. ALOX5 Glu254Lys and COX2 C-645T and Val511Ala allele frequencies differed between Caucasians and African-American controls (P < 0.001). The ALOX5 -1752 and -1699 polymorphisms were in linkage disequilibrium (P < 0.001) and associated with a decreased risk in Caucasians in ALOX5 haplotype analyses (P = 0.03). Furthermore, an inverse association was observed between A alleles at positions -1752 and -1699 of ALOX5 and colon cancer risk in Caucasians, but not in African-Americans. Caucasians with A alleles at ALOX5 -1752 had a reduced odds of colon cancer versus those with G alleles [odds ratio (OR) (GA versus GG), 0.63; 95% confidence interval (CI), 0.39-1.01; OR (AA versus GG), 0.33; 95% CI, 0.07-1.65, P(trend) = 0.02]. Similar results were observed for ALOX5 G-1699A [OR (GA versus GG), 0.59, 95% CI, 0.37-0.94; OR (AA versus GG), 0.27, 95% CI, 0.06-1.32, P(trend) = 0.01]. Statistically significant associations with colon cancer were not observed for the other polymorphisms investigated. We have shown for the first time that a haplotype containing ALOX5 G-1752A and G-1699A in a negative regulatory region of the promoter may influence colon cancer risk in Caucasians.  (+info)

Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality. (8/145)

Lipoxygenases (LOXs) catalyze the regio- and stereospecific dioxygenation of polyunsaturated membrane-embedded fatty acids. We report here the 3.2 A resolution structure of 8R-LOX from the Caribbean sea whip coral Plexaura homomalla, a LOX isozyme with calcium dependence and the uncommon R chiral stereospecificity. Structural and spectroscopic analyses demonstrated calcium binding in a C2-like membrane-binding domain, illuminating the function of similar amino acids in calcium-activated mammalian 5-LOX, the key enzyme in the pathway to the pro-inflammatory leukotrienes. Mutation of Ca(2+)-ligating amino acids in 8R-LOX resulted not only in a diminished capacity to bind membranes, as monitored by fluorescence resonance energy transfer, but also in an associated loss of Ca(2+)-regulated enzyme activity. Moreover, a structural basis for R chiral specificity is also revealed; creation of a small oxygen pocket next to Gly(428) (Ala in all S-LOX isozymes) promoted C-8 oxygenation with R chirality on the activated fatty acid substrate.  (+info)

*Arachidonate 5-lipoxygenase

... , also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13 ... Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. It transforms essential fatty acids (EFA) ... "Alox5 - arachidonate 5-lipoxygenase". WikiGenes. Fahel JS, de Souza MB, Gomes MT, Corsetti PP, Carvalho NB, Marinho FA, de ... Arachidonate 5-Lipoxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Human ALOX5 genome location ...

*Arachidonate 8-lipoxygenase

... (EC 1.13.11.40) is an enzyme that catalyzes the chemical reaction arachidonate + O2 ⇌ {\ ... Other names in common use include 8-lipoxygenase, and 8(R)-lipoxygenase. This enzyme participates in arachidonic acid ... Bundy GL, Nidy EG, Epps DE, Mizsak SA, Wnuk RJ (1986). "Discovery of an arachidonic acid C-8 lipoxygenase in the gorgonian ... The systematic name of this enzyme class is arachidonate:oxygen 8-oxidoreductase. ...

*Arachidonate 5-lipoxygenase inhibitor

... s are compounds that slow or stop the action of the arachidonate 5-lipoxygenase (5- ... Arachidonate 5-lipoxygenase ...Specific function: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a ... Lipoxygenase inhibitors at the US National Library of Medicine Medical Subject Headings (MeSH) MeSH list of agents 82016859. ... Zileuton is a specific inhibitor of 5-lipoxygenase and thus inhibits leukotriene (LTB4, LTC4, LTD4, and LTE4) formation. Both ...

*ALOXE3

... arachidonate lipoxygenase 3". Schneider C, Brash AR (August 2002). "Lipoxygenase-catalyzed formation of R-configuration ... Epidermis-type lipoxygenase 3 (ALOXE3 or eLOX3) is a member of the lipoxygenase family of enzymes; in humans, it is encoded by ... The epidermis-type lipoxygenases are now regarded as a distinct subclass within the multigene family of mammalian lipoxygenases ... "Lipoxygenase-3 (ALOXE3) and 12(R)-lipoxygenase (ALOX12B) are mutated in non-bullous congenital ichthyosiform erythroderma (NCIE ...

*12-Hydroxyeicosatetraenoic acid

In humans, Arachidonate 12-lipoxygenase (12-LO, 12-LOX, ALO12, or platelet type 12-lipoxygenase) is encoded by the ALOX12 gene ... 12-lipoxygenase gene, see lipoxygenase#mouse lipoxygenases) are resistant to a) streptozotocin-induced, b) high fat diet- ... Arachidonate 12-lipoxygenase, 12R type, also termed 12RLOX and encoded by the ALOX12B gene, is expressed primarily in skin and ... express platelet type 12-lipoxygenase but also a leukocyte type 12-lipoxygenase (also termed 12/15-lipoxygenase, 12/15-LOX or ...

*ALOX12

... also known as arachidonate 12-lipoxygenase, 12-lipoxygenase, 12S-Lipoxygenase, 12-LOX, and 12S-LOX is a lipoxygenase-type ... "Entrez Gene: ALOX12 arachidonate 12-lipoxygenase". Yamamoto S, Suzuki H, Ueda N (March 1997). "Arachidonate 12-lipoxygenases". ... arachidonate:oxygen 12-oxidoreductase, Delta12-lipoxygenase, 12Delta-lipoxygenase, C-12 lipoxygenase, leukotriene A4 synthase, ... Nakamura M, Yamamoto S, Ishimura K (May 1997). "Subcellular localization of arachidonate 12-lipoxygenase and morphological ...

*15-Hydroxyeicosatetraenoic acid

"Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells". Journal ... 15-Lipoxygenase-1: Cells metabolize arachidonic acid with 15-lipoxygenase-1 (i.e., 15-LO-1, ALOX15) to form 15(S)-HpETE as a ... an arachidonate lipoxygenase product". Biochimica et Biophysica Acta. 1136 (3): 247-52. doi:10.1016/0167-4889(92)90113-p. PMID ... inhibitor of 12-lipoxygenase but not other human lipoxygenases. This effect could also have anti-inflammatory and anti- ...

*Hypericum perforatum

In humans, the active ingredient hyperforin is also an inhibitor of PTGS1, arachidonate 5-lipoxygenase, SLCO1B1 and an inducer ... It is a medicinal herb with antidepressant activity and anti-inflammatory properties as an arachidonate 5-lipoxygenase ... Arachidonate 5-lipoxygenase ...Specific function: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a ... and 5-lipoxygenase at low concentrations of 0.3 µmol/L and 1.2 µmol/L, respectively [52], and of PGE2 production in vitro [53] ...

*Antileukotriene

An antileukotriene is a drug which functions as a leukotriene-related enzyme inhibitor (arachidonate 5-lipoxygenase) or ... Arachidonate 5-lipoxygenase ...Specific function: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a ... drugs such as MK-886 that block the 5-lipoxygenase activating protein (FLAP) inhibit functioning of the 5-lipoxygenase enzyme ... Zileuton is a specific inhibitor of 5-lipoxygenase and thus inhibits leukotriene (LTB4, LTC4, LTD4, and LTE4) formation. Both ...

*ALOX12B

Arachidonate 12-lipoxygenase, 12R type, also known as ALOX12B, 12R-LOX, and arachiconate lipoygenase 3, is a lipoxygenase-type ... ALOX12B arachidonate 12-lipoxygenase, 12R type". Boeglin WE, Kim RB, Brash AR (June 1998). "A 12R-lipoxygenase in human skin: ... "Lipoxygenase-3 (ALOXE3) and 12(R)-lipoxygenase (ALOX12B) are mutated in non-bullous congenital ichthyosiform erythroderma (NCIE ... Among the human lipoxygenases, ALOX12B is most closely (54% identity) related in amino acid sequence to ALOXE3 ALOX12B ...

*Eicosanoid

ALOX12B (i.e. arachidonate 12-lipoxygenase, 12R type) forms R chirality products, i.e. 12R-HpETE and 12R-HETE. Similarly, ... The 15-lipoxygenases (particularly ALOX15) may also act in series with 5-lipoxygenase, 12-lipoxygenase, or aspirin-treated COX2 ... The enzymes 15-lipoxygenase-1 (15-LO-1 or ALOX15) and 15-lipoxygenase-2 (15-LO-2, ALOX15B) metabolize arachidonic acid to the S ... They include (HEPE is hydroxy-eicsapentaenoic acid): 5-HEPE (see Arachidonate 5-lipoxygenase#Eicosapentaenoic acid), 12-HEPE, ...

*Prostate cancer

Arachidonate 5-lipoxygenase has been identified as playing a significant role in the survival of prostate cancer cells. ... In particular, arachidonate 5-lipoxygenase inhibitors produce massive, rapid programmed cell death in prostate cancer cells. ... Ghosh J, Myers CE (October 1998). "Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate ... The 5-lipoxygenase (5-LOX) pathway is implicated in the development and progression of human cancers. 5-LOX, whose crystal ...

*Oxoeicosanoid receptor 1

Ghosh J, Myers CE (1998). "Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells ... mediates the survival-promoting effects of arachidonate 5-lipoxygenase in prostate cancer cells". Cancer Lett. 336 (1): 185-95 ... Hussey HJ, Tisdale MJ (1996). "Inhibition of tumour growth by lipoxygenase inhibitors". Br. J. Cancer. 74 (5): 683-687. doi: ... Wijkander J, O'Flaherty JT, Nixon AB, Wykle RL (1995). "5-Lipoxygenase products modulate the activity of the 85-kDa ...

*Leukotriene

... s are synthesized in the cell from arachidonic acid by arachidonate 5-lipoxygenase. The catalytic mechanism involves ... and donated by the 5-lipoxygenase-activating protein (FLAP) to 5-lipoxygenase.[citation needed] 5-Lipoxygenase (5-LO) uses FLAP ... by the enzyme arachidonate 5-lipoxygenase. Leukotrienes use lipid signaling to convey information to either the cell producing ... Recent research points to a role of 5-lipoxygenase in cardiovascular and neuropsychiatric illnesses. Leukotrienes are very ...

*List of MeSH codes (D12.776)

... arachidonate 5-lipoxygenase MeSH D12.776.556.579.374.450.025.025 - arachidonate 12-lipoxygenase MeSH D12.776.556.579.374.450. ... lipoxygenase MeSH D12.776.556.579.374.450.025 - arachidonate lipoxygenases MeSH D12.776.556.579.374.450.025.020 - ... arachidonate 15-lipoxygenase MeSH D12.776.556.579.374.687 - retinal dehydrogenase MeSH D12.776.556.579.374.925 - tyrosine 3- ...

*List of MeSH codes (D08)

... arachidonate 5-lipoxygenase MeSH D08.811.682.690.416.444.050.060 --- arachidonate 12-lipoxygenase MeSH D08.811.682.690.416.444. ... lipoxygenase MeSH D08.811.682.690.416.444.050 --- arachidonate lipoxygenases MeSH D08.811.682.690.416.444.050.055 --- ... arachidonate 15-lipoxygenase MeSH D08.811.682.690.416.444.525 --- protocatechuate 3,4-dioxygenase MeSH D08.811.682.690.416.722 ...

*Eoxin

Arachidonate 5-lipoxygenase Arachidonate 15-lipoxygenase Leukotriene 15-Hydroxyicosatetraenoic acid Greene ER, Huang S, Serhan ... The primary product of the lipoxygenase, 15-HPETE is believed to react with the enzyme further to produce the 14,15-epoxide, ... Claesson HE (September 2009). "On the biosynthesis and biological role of eoxins and 15-lipoxygenase-1 in airway inflammation ... Forsell PK, Brunnström A, Johannesson M, Claesson HE (2012). "Metabolism of anandamide into eoxamides by 15-lipoxygenase-1 and ...

*5-lipoxygenase-activating protein

Arachidonate 5-lipoxygenase-activating protein also known as 5-lipoxygenase activating protein, or FLAP, is a protein that in ... Zintzaras E, Rodopoulou P, Sakellaridis N (March 2009). "Variants of the arachidonate 5-lipoxygenase-activating protein ( ... "Associations of genetic polymorphisms of arachidonate 5-lipoxygenase-activating protein with risk of coronary artery disease in ... "Cytogenetic and radiation hybrid mapping of human arachidonate 5-lipoxygenase-activating protein (ALOX5AP) to chromosome 13q12 ...

*ALOX15B

Arachidonate 15-lipoxygenase type II is an enzyme that in humans is encoded by the ALOX15B gene. ALOX15B, also known as 15- ... "Entrez Gene: ALOX15B arachidonate 15-lipoxygenase, type B". Human ALOX15B genome location and ALOX15B gene details page in the ... Arachidonate 15-lipoxygenase 15-hydroxyicosatetraenoic acid ALOX15 GRCh38: Ensembl release 89: ENSG00000179593 - Ensembl, May ... "Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells". The ...

*Perovskia atriplicifolia

The isorinic acid derivative perovskoate may also contribute to an anti-inflammatory effect as an arachidonate 5-lipoxygenase ...

*Gamma-Linolenic acid

When acting on GLA, Arachidonate 5-lipoxygenase produces no leukotrienes and the conversion by the enzyme of arachidonic acid ...

*Lipoxygenase

Arachidonate 15-lipoxygenase type II (ALOX15B), also termed 15-lipoxygenase-2, 15-LOX-2, and 15-LOX-2. It metabolizes ... soybean lipoxygenase L1 and L3, coral 8-lipoxygenase, human 5-lipoxygenase, rabbit 15-lipoxygenase and porcine leukocyte 12- ... erythrocyte type 15-lipoxygenase (or 15-lipoxygenase, erythrocyte type), reticulocyte type 15-lipoxygenase (or 15-lipoxygenase ... Arachidonate 12-lipoxygenase (ALOX12) (EC 1.13.11.31InterPro: IPR001885), also termed 12-lipoxygenase, platelet type platelet ...

*Curcumin

... and the arachidonate 5-lipoxygenase enzyme. Factors that limit the bioactivity of curcumin or its analogs include chemical ... Bishayee, K.; Khuda-Bukhsh, A. R. (September 2013). "5-lipoxygenase antagonist therapy: a new approach towards targeted cancer ...

*5-oxo-eicosatetraenoic acid

First, human eosinophils use Arachidonate 15-lipoxygenase-1 (or possibly Arachidonate 15-lipoxygenase-2 to metabolize 5-oxo-ETE ... oxygenation of this arachidonic acid with by activated arachidonate 5-lipoxygenase (ALOX5) to form 5(S)-hydroperoxy-6E,8Z,11Z, ... Second, human platelets use 12-lipoxygenase to metabolize 5-oxo-ETE to 5-oxo-12(S)-hydroperxy-eicosatetraenoat which is rapidly ... This transcellular production typically involves the limited variety of cell types that express active 5-lipoxygenase, lack ...

*ALOX15

... arachidonate 15-lipoxygenase 2, 15-lipoxygenase-2, 15-LOX-2, 15-LO-2, arachidonate 15-lipoxygenase type II, arachidonate 15- ... 15-lipoxygenase-1, 15-LOX-1, 15-LO-1, human 12/15-lipoxygenase, leukocyte-type arachidonate 12-lipoxygenase, or arachidonate ... ALOX15 (also termed arachidonate 15-lipoxygenase, 15-lipoxygenase-1, 15-LO-1, 15-LOX-1) is, like other lipoxygenases, a seminal ... Sigal E, Grunberger D, Craik CS, Caughey GH, Nadel JA (April 1988). "Arachidonate 15-lipoxygenase (omega-6 lipoxygenase) from ...

*Mead acid

ISBN 9 78-0-19-876802-9. Phinney SD, Odin RS, Johnson SB, Holman RT (1990). "Reduced arachidonate in serum phospholipids and ... Wei YF, Evans RW, Morrison AR, Sprechert H, Jakschik BA (1985). "Double bond requirement for the 5-lipoxygenase pathway". ... Mead acid is metabolized by 5-lipoxygenase to 5-hydroxyeicosatrieonic acid (5-HETrE) and then by 5-Hydroxyeicosanoid ... In the presence of lipoxygenase, cytochrome p450 or cyclooxygenase Mead acid can form various hydroxyeicosatetraenoic acid ( ...
Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
sp:LIDS_GAEGR] linoleate 8R-lipoxygenase / 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase (EC:1.13.11.60 5.4.4.6); K17864 linoleate 8R-lipoxygenase / 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase [EC:1.13.11.60 5.4.4.6] ...
sp:LIDS_GAEGR] linoleate 8R-lipoxygenase / 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase (EC:1.13.11.60 5.4.4.6); K17864 linoleate 8R-lipoxygenase / 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase [EC:1.13.11.60 5.4.4.6] ...
...UPTON NY - Scientists at the U.S. Department of Energys Brookhaven N... Those properties make this new form of carbon particularly attractive...Supercapacitors are similar to batteries in that both store electric c...Supercapacitors on the other hand store charge in the form of ions o...,Activated,graphene,makes,superior,supercapacitors,for,energy,storage,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
Formation of the 12R-lipoxygenase product, 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE), has been detected previously only in human skin (Boeglin et al. (1998) Proc. Natl. Acad. Sci. USA 95, 6744). The unexpected appearance of an EST sequence (AA649213) for human 12R-lipoxygenase from germinal center B lymphocytes purified from human tonsils prompted our search for the existence of the enzyme in this novel source. Incubation of [1-14C]arachidonic acid with homogenates of human tonsillar tissue yielded mixtures of radiolabeled 12-HETE and 15-HETE. Stereochemical analysis showed varying ratios of 12S- and 12R-HETE, while 15-HETE was exclusively of the S-configuration. Using stereospecifically labeled [10S-3H]- and [10R-3H]arachidonic acid substrates we detected pro-R hydrogen abstraction at carbon 10 associated with formation of 12R-HETE. This mechanistic evidence implicates a 12R-lipoxygenase in the biosynthesis of 12R-HETE. The mRNA for the enzyme was identified in tonsils by RT-PCR and ...
Learn about the potential side effects of acetohydroxamic acid. Includes common and rare side effects information for consumers and healthcare professionals.
Learn about Lithostat (Acetohydroxamic Acid Tablets) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, reviews, and related medications.
The enzyme contains heme. The bifunctional enzyme from Aspergillus nidulans uses different heme domains to catalyse two separate reactions. Linoleic acid is oxidized with
In contrast to the well-studied role of 5-lipoxygenase in the arachidonic acid cascade that occurs in inflammatory cells, the biological role of the related 15-lipoxygenases in the metabolism of free polyenoic fatty acids is far from clear. However, the activity of 15-lipoxygenases with more complex substrates may play a crucial role in the differentiation and maturation of certain cell types and in the oxidative modification of lipoproteins in the early stages of atherosclerosis ...
Royo, J., Vancanneyt, G., Perez, A.G., Sanz, C., Stormann, K., Rosahl, S. and Sanchez-Serrano, J.J. (1996). „Characterization of three potato lipoxygenases with distinct enzymatic activities and different organ-specific and wound-regulated expression patterns". J. Biol. Chem. 271: 21012-21019. PMID 8702864 ...
ethyl arachidonate 1808-26-0 NMR spectrum, ethyl arachidonate H-NMR spectral analysis, ethyl arachidonate C-NMR spectral analysis ect.
abbr.: diHETE; any of several products resulting from dioxygenation of arachidonate at two sites, normally resulting from lipoxygenase action on hydroxyeicosatetraenoates. (6E,8Z,10E,14Z)‐(5S,12S)‐5,12‐dihydroxyeicosa‐6,8,10,14‐tetraen‐1‐oate (abbr.: 5(S),12(S)‐diHETE) is synthesized in human leukocytes by ... ...
Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins ...
Lipoxygenases (LOXs) are lipid peroxidizing enzymes, implicated in the pathogenesis of inflammatory and hyperproliferative diseases, which represent potential targets for pharmacological intervention. Although soybean LOX1 was discovered more than 60 years ago, the structural biology of these enzymes was not studied until the mid 1990s. In 1993 the first crystal structure for a plant LOX was solved and following this protein biochemistry and molecular enzymology became major fields in LOX research. This review focuses on recent developments in molecular enzymology of LOXs and summarizes our current understanding of the structural basis of LOX catalysis. Various hypotheses explaining the reaction specificity of different isoforms are critically reviewed and their pros and cons briefly discussed. Moreover, we summarize the current knowledge of LOX evolution by profiling the existence of LOX-related genomic sequences in the three kingdoms of life. Such sequences are found in eukaryotes and bacteria ...
Lipoxygenases (LOs) constitute a heterogeneous family of enzymes that catalyze the stereoselective dioxygenation of polyunsaturated fatty acids to their corresponding hydroperoxy derivatives.1,2 In mammals, LOs are categorized with respect to their positional specificity of arachidonic acid oxygenation into 5-, 8-, 12-, and 15-LOs.1-3 These enzymes induce structural and metabolic changes in cells during a wide variety of physiological and pathological processes, such as differentiation, carcinogenesis, inflammation, and atherogenesis. Accumulating studies have suggested diverse and opposing roles for the various LO pathways in the pathogenesis of human diseases, particularly cancer and atherosclerosis.2-5 Consistent with the existence of multiple isoforms of LOs, a variety of intermediate and end products of arachidonic acids are found in various cell types, and they in turn activate diverse signaling cascades, resulting in diverse outcomes. Therefore, sophisticated understanding of the ...
Glycine max (1yge, 1f8n, 1fgm, 1fgo, 1fgq, 1fgr, 1fgt, 1y4k, 2sbl, 3bnb, 3bnc, 3bnd, 3bne, 3pzw, 4wfo, 4wha, 5eeo, 5t5v, 5tqn, 5tqo, 5tqp, 5tr0 ...
During oxygenation by 15-lipoxygenases, polyenoic fatty acids are bound at the active site in such a way that the ω-terminus of the fatty acids penetrates into the substrate binding pocket. In contrast, for arachidonic acid 5-lipoxygenation, an inverse head to tail orientation has been suggested. However, an inverse orientation may be hindered by the large energy barrier associated with burying the charged carboxylate group in the hydrophobic environment of the substrate binding cleft. We studied the oxygenation kinetics of ω-modified fatty acids by 15-lipoxygenases and found that ω-hydroxylation strongly impaired substrate affinity (higher Km), but only moderately altered Vmax. In contrast, ω-carboxylation completely prevented the lipoxygenase reaction; however, methylation of the additional carboxylate group restored the activity. Arg403 of the human 15-lipoxygenase has been implicated in fatty acid binding by forming a salt bridge with the carboxylate group, and thus mutation of this ...
12-Hydroxyeicosatetraenoic acid (12-HETE) is a derivative of the 20 carbon polyunsaturated fatty acid, arachidonic acid, containing a Hydroxyl residue at carbon 12 and a 5Z,8Z,10E,14Z Cis-trans isomerism configuration (Z=cis, E=trans) in its four double bonds. It was first found as a product of arachidonic acid metabolism made by human and bovine platelets through their 12S-lipoxygenase (i.e. ALOX12) enzyme(s). However, the term 12-HETE is ambiquous in that it has been used to indicate not only the initially detected "S" stereoisomer, 12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (12(S)-HETE or 12S-HETE), made by platelets, but also the later detected "R" stereoisomer, 12(R)-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (also termed 12(R)-HETE or 12R-HETE) made by other tissues through their 12R-lipoxygenase enzyme, ALOX12B. The two isomers, either directly or after being further metabolized, have been suggested to be involved in a variety of human physiological and pathological reactions. ...
15 Lipoxygenase 1小鼠单克隆抗体[3G8](ab119774)可与大鼠, 狗, 人, 猴样本反应并经WB, IHC, ICC/IF实验严格验证。所有产品均提供质保服务,中国75%以上现货。
References for Abcams Recombinant Human 5 Lipoxygenase protein (ab114310). Please let us know if you have used this product in your publication
References for Abcams Recombinant Human 15 Lipoxygenase 1 protein (ab114421). Please let us know if you have used this product in your publication
Discovery of selective imidazole-based inhibitors of mammalian 15-lipoxygenase: Highly potent against human enzyme within a cellular environment ...
Looking for online definition of 5,8,11,14-Eicosatetraenoic acid in the Medical Dictionary? 5,8,11,14-Eicosatetraenoic acid explanation free. What is 5,8,11,14-Eicosatetraenoic acid? Meaning of 5,8,11,14-Eicosatetraenoic acid medical term. What does 5,8,11,14-Eicosatetraenoic acid mean?
Eoxins are proposed to be a family of proinflammatory eicosanoids (signaling compounds that regulate inflammatory and immune responses). They are produced by human eosinophils (a class of white blood cells), mast cells, the L1236 Reed-Sternberg cell line derived from Hodgkins Lymphoma, and certain other tissues tissue. These cells produce the eoxins by initially metabolizing arachidonic acid, an omega-6 (ω-6) fatty acid, via any enzyme possessing 15-lipoxygenase activity. The product of this initial metabolic step, 15(S)-hydroperoxyeicosatetraenoic acid, is then converted to a series of eoxins by the same enzymes that metabolize the 5-lipoxygenase product of arachidonic acid metabolism, i.e. 5-hydroperoxy-eicosatetraenoic acid to a series of leukotrienes. That is, the eoxins are 14,15-disubstituted analogs of the 5,6-disubstituted leukotrienes. A closely related set of 15-lipoxygenase metabolites are derived from anandamide (i.e. arachidonic acid containing ethanolamine esterified to its ...
N. Topley, R. Steadman, B. Spur, J.D. Williams; Independent Activation of the 5-Lipoxygenase Pathway and Respiratory Burst in Human Polymorphonuclear Leukocytes (PMN) in Response to Phagocytic Stimuli. Clin Sci (Lond) 1 January 1988; 74 (s18): 65P-66P. doi: https://doi.org/10.1042/cs074065Pc. Download citation file:. ...
BioAssay record AID 3623 submitted by ChEMBL: Inhibition of [14C]arachidonic acid conversion to 5-HETE by broken cell 5-LO isolated from guinea pig PMN.
Bryant, R.W., Bailey, J.M., Schewqe, T. and Rapoport, S.M. (1982). „Positional specificity of a reticulocyte lipoxygenase. Conversion of arachidonic acid to 15-S-hydroperoxy-eicosatetraenoic acid". J. Biol. Chem. 257: 6050-6055. PMID 6804460 ...
Looking at my sequence of animals, there are a few conclusions I can come to. One conclusion I can come to is that my protein, lipoxygenase, must be very common because a variety of animals are in the sequence you may not think of as relatives. Sense lipoxygenase is involved in the metabolism that shows…
TY - JOUR. T1 - Role of epidermis-type lipoxygenases for skin barrier function and adipocyte differentiation. AU - Fürstenberger, Gerhard. AU - Epp, Nikolas. AU - Eckl, Katja Martina. AU - Hennies, Hans Christian. AU - Jørgensen, Claus. AU - Hallenborg, Philip. AU - Kristiansen, Karsten. AU - Krieg, Peter. PY - 2007/1/1. Y1 - 2007/1/1. N2 - 12R-lipoxygenase (12R-LOX) and epidermis-type LOX-3 (eLOX-3) are novel members of the multigene family of mammalian LOX. A considerable gap exists between the identification of these enzymes and their biologic function. Here, we present evidence that 12R-LOX and eLOX-3, acting in sequence, and eLOX-3 in combination with another, not yet identified LOX are critically involved in terminal differentiation of keratinocytes and adipocytes, respectively. Mutational inactivation of 12R-LOX and/or eLOX-3 has been found to be associated with development of an inherited ichthyosiform skin disorder in humans and genetic ablation of 12R-LOX causes a severe impairment ...
The 5-lipoxygenase (5-LO) product 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE), which is a potent chemoattractant for myeloid cells, is known to promote the survival of prostate cancer cells. In the present study, we found that PC3 prostate cancer cells and cell lines derived from breast (MCF7) and lung (A-427) cancers contain 5-hydroxyeicosanoid dehydrogenase (5-HEDH) activity and have the ability to synthesize 5-oxo-ETE from its precursor 5S-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE) when added as an exogenous substrate. H2O2 strongly stimulated the synthesis of 5-oxo-ETE and induced dramatic increases in the levels of both glutathione disulfide and NADP+. The effects of H2O2 on 5-oxo-ETE and NADP+ were blocked by N-ethylmaleimide (NEM), indicating that this effect was mediated by the glutathione reductase-dependent generation of NADP+, the cofactor required by 5-HEDH. 5-Oxo-ETE synthesis was also stimulated by agents that have cytotoxic effects on tumor cells, including ...
The invention is directed to the enhanced expression and purification of lipoxygenase enzymes. These enzymes are of wide-spread industrial importance, often produced in heterologous microbial systems. Preferably, the lipoxygenase produced by the methods of the invention is a plant-derived enzyme and expressed at high-levels in a microbial system that includes a protease-deficient host and one or more chaperone expression plasmids. The invention is also directed to amino acid and nucleic acid fragments of the lipoxygenase enzyme including fragments in expression constructs encoding all or a portion of one or more lipoxygenase genes. The invention is also directed to methods of manufacturing bread and other food and also non-food products with lipoxygenase manufactured by the methods of the invention.
The viability of different mechanisms of catalysis and inhibition of the nickel-containing enzyme urease was explored using the available high-resolution structures of the enzyme isolated from Bacillus pasteurii in the native form and inhibited with several substrates. The structures and charge distribution of urea, its catalytic transition state, and three enzyme inhibitors were calculated using ab initio and density functional theory methods. The DOCK program suite was employed to determine families of structures of urease complexes characterized by docking energy scores indicative of their relative stability according to steric and electrostatic criteria. Adjustment of the parameters used by DOCK, in order to account for the presence of the metal ion in the active site, resulted in the calculation of best energy structures for the nickel-bound inhibitors β-mercaptoethanol, acetohydroxamic acid, and diamidophosphoric acid. These calculated structures are in good agreement with the experimentally
A1777 is a selective 5-lipoxygenase inhibitor that reduces leukocytes proliferation without affecting the eosinophils of mast cells.
Madeswaran, Arumugam, Muthuswamy Umamaheswari, Kuppuswamy Asokkumar, Thirumalaisamy Sivashanmugam, Varadharajan Subhadradevi, Puliyath Jagannath (2011) Docking studies: In silico lipoxygenase inhibitory activity of some commercially available flavonoids. [Publication] Full text not available from this repository ...
1yge, 1f8n, 1fgm, 1fgo, 1fgq, 1fgr, 1fgt, 1y4k, 2sbl, 3bnb, 3bnc, 3bnd, 3bne, 3pzw, 4wfo, 4wha, 5eeo, 5t5v, 5tqn, 5tqo, 5tqp, 5tr0 ...
In the United States, one third of the American adults have high blood pressure or hypertension, and among those, almost one third even do not know that they had the condition. More.... ...
Recently, we have demonstrated by two different methods that lipoxgenases (LOXs) and 14-3-3 proteins form interactions in barley embryos [Holtman, Roberts, Oppedijk, Testerink, van Zeijl and Wang (2000) FEBS Lett. 474, 48-52]. It was shown by both co-immunoprecipitations and surface-plasmon resonance experiments that 13-LOX, but not 9-LOX, forms interactions with 14-3-3 proteins. In the present report we show that the presence of 13-LOX and 14-3-3 proteins was established in high-molecular-mass complexes. Amounts of 13-LOX and 14-3-3 proteins in high-molecular-mass fractions increased during germination, but were reduced after dephosphorylation of protein extracts or competition with the 14-3-3-binding peptide P-Raf-259, indicating that 13-LOX and 14-3-3 proteins interact in a phosphorylation-dependent manner. Chemicals/CAS: 13-lipoxygenase, EC 1.13.11.-; 14-3-3 Proteins; Lipoxygenase, EC 1.13.11.12; Tyrosine 3-Monooxygenase, EC 1.14.16.2 ...
Gingerforce modulates the 5-lipoxygenase enzyme. Garlicforce by New Chapter Supports stomach, liver, and intestinal health. Garlicforce Supports blood platelet health and cardiovascular function and Promotes healthy inflammation response.
Contact Us. Tel:732-484-9848. Fax:888-484-5008. Email:[email protected]. Add:1 Deer Park Dr, Suite Q,. Monmouth Junction, NJ 08852, USA. ...
Enzymes of wheat flour have an important role in production of various cereal products, especially bread, and therefore accurate measurement of enzymatic activity along with improvement of this characteristic is the basis of most studies in baking industry. In the present work, physical properties of three wheat varieties (Mahdavi, Kavir ...
Inflammation in the vascular wall is important for development of atherosclerosis. We have shown previously that arachidonate 15-lipoxygenase type B (ALOX15B) is more highly expressed in human atherosclerotic lesions than in healthy arteries. This en
Bermudez, AF, Coles, B, Coffey, M and ODonnell, V (2003) Protein kinase C regulates 15-lipoxygenase oxidation of membrane-bound arachidonate in human monocytes In: 10th Annual Meeting of the Society-for-Free-Radical-Biology-and-Medicne, 2003-11-20 - 2003-11-24, SEATTLE, WASHINGTON. Full text not available from this repository ...
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Stimulated Human Colonic Synthesis of Arachidonate Lipoxygenase and Cyclo-Oxygenase Products by Laxatives | SpringerLinkStimulated Human Colonic Synthesis of Arachidonate Lipoxygenase and Cyclo-Oxygenase Products by Laxatives | SpringerLink

We have recently demonstrated that rat colon can convert 14Carachidonic acid into both lipoxygenase and cyclo-oxygenase ... Capasso F., Tavares I.A., Bennett A. (1985) Stimulated Human Colonic Synthesis of Arachidonate Lipoxygenase and Cyclo-Oxygenase ... Stimulated Human Colonic Synthesis of Arachidonate Lipoxygenase and Cyclo-Oxygenase Products by Laxatives. ... Arachidonic Acid Ricinoleic Acid Arachidonic Acid Metabolism Lipoxygenase Product Sodium Sulfosuccinate These keywords were ...
more infohttps://link.springer.com/chapter/10.1007%2F978-1-4684-7841-9_28

KAKEN - Research Projects | Molecular pathophysiological studies on arachidonate lipoxygenases (KAKENHI-PROJECT-07044273)KAKEN - Research Projects | Molecular pathophysiological studies on arachidonate lipoxygenases (KAKENHI-PROJECT-07044273)

Publications] T.Arakawa: The transcriptional regulation of human arachidonate 12-lipoxygenase gene by NFκB/Rel FEBS Lett.363 ... Publications] T.Arakawa: The transcriptional regulation of human arachidonate 12-lipoxygenase gene by NFkB/Rel FEBS Lett.363 ... 15-Lipoxygenase is now under investigations.. 3) 12-Lipoxygenase of human uterine cervix : Nigams group of Free University of ... Publications] M.Nakamura: Immunocytochemical localization of platelet-type arachidonate 12-lipoxygenase in mouse blood cells ...
more infohttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044273/

Arachidonate 5-lipoxygenase - WikipediaArachidonate 5-lipoxygenase - Wikipedia

Arachidonate 5-lipoxygenase, also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13 ... that in humans is encoded by the ALOX5 gene.[5] Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. ... "Alox5 - arachidonate 5-lipoxygenase". WikiGenes.. *^ Fahel JS, de Souza MB, Gomes MT, Corsetti PP, Carvalho NB, Marinho FA, de ... arachidonate 5-lipoxygenase activity. • dioxygenase activity. • metal ion binding. • protein binding. • oxidoreductase activity ...
more infohttps://en.wikipedia.org/wiki/Arachidonate_5-lipoxygenase

Arachidonate 5-lipoxygenase - WikipediaArachidonate 5-lipoxygenase - Wikipedia

Arachidonate 5-lipoxygenase, also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13 ... that in humans is encoded by the ALOX5 gene.[5] Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. ... "Alox5 - arachidonate 5-lipoxygenase". WikiGenes.. *^ Fahel JS, de Souza MB, Gomes MT, Corsetti PP, Carvalho NB, Marinho FA, de ... arachidonate 5-lipoxygenase activity. • dioxygenase activity. • metal ion binding. • protein binding. • oxidoreductase activity ...
more infohttps://en.m.wikipedia.org/wiki/5-Lipoxygenase

Effects of the Arachidonate Lipoxygenase Inhibitor BW755C on Traumatic and Peritumoural Brain Oedema | Springer for Research &...Effects of the Arachidonate Lipoxygenase Inhibitor BW755C on Traumatic and Peritumoural Brain Oedema | Springer for Research &...

It inhibits synthesis of prostaglandins and leukotrienes by inhibition in the cyclooxygenase and lipoxygenase pathways of... ... Effects of the Arachidonate Lipoxygenase Inhibitor BW755C on Traumatic and Peritumoural Brain Oedema. ... Higgs GA, Mugridge KG, Moncada S, Vane JR (1984) Inhibition of tissue damage by the arachidonate lipoxygenase inhibitor BW755C ... Ikeda Y., Long D.M. (1990) Effects of the Arachidonate Lipoxygenase Inhibitor BW755C on Traumatic and Peritumoural Brain Oedema ...
more infohttps://rd.springer.com/chapter/10.1007/978-3-7091-9115-6_23

RCSB PDB - Protein Feature View 









 - Arachidonate 5-lipoxygenase - P09917 (LOX5 HUMAN)RCSB PDB - Protein Feature View - Arachidonate 5-lipoxygenase - P09917 (LOX5 HUMAN)

The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
more infohttp://www.rcsb.org/pdb/protein/E5FPY8

Arachidonate 8-lipoxygenase - WikipediaArachidonate 8-lipoxygenase - Wikipedia

Arachidonate 8-lipoxygenase (EC 1.13.11.40) is an enzyme that catalyzes the chemical reaction arachidonate + O2 ⇌ {\ ... Other names in common use include 8-lipoxygenase, and 8(R)-lipoxygenase. This enzyme participates in arachidonic acid ... Bundy GL, Nidy EG, Epps DE, Mizsak SA, Wnuk RJ (1986). "Discovery of an arachidonic acid C-8 lipoxygenase in the gorgonian ... The systematic name of this enzyme class is arachidonate:oxygen 8-oxidoreductase. ...
more infohttps://en.wikipedia.org/wiki/Arachidonate_8-lipoxygenase

RCSB PDB - Protein Feature View 









 - Arachidonate 15-lipoxygenase - P27479 (LOX15 BOVIN)RCSB PDB - Protein Feature View - Arachidonate 15-lipoxygenase - P27479 (LOX15 BOVIN)

Arachidonate + O2 = 5Z,8Z,11Z,13E-15S-15-hydroperoxyicosa-5,8,11,13-tetraenoate. UniProt ...
more infohttp://www.rcsb.org/pdb/protein/P27479

ALOX12 (Arachidonate 12-Lipoxygenase) Homo sapiensALOX12 (Arachidonate 12-Lipoxygenase) Homo sapiens

Arachidonate 12-Lipoxygenase) Homo sapiens, Authors: Sreeparna Banerjee, Asli Erdog. Published in: Atlas Genet Cytogenet Oncol ... ALOX12 17p13.1 arachidonate 12-lipoxygenase (Homo sapiens), ALOX12P2 17p13 arachidonate 12-lipoxygenase pseudogene 2, TEKT1 ... arachidonate 12-lipoxygenase activity iron ion binding protein binding cytoplasm cytosol cytosol aging positive regulation of ... arachidonate 12-lipoxygenase activity iron ion binding protein binding cytoplasm cytosol cytosol aging positive regulation of ...
more infohttp://atlasgeneticsoncology.org/Genes/ALOX12ID620ch17p13.html

ALOX15 - arachidonate 15-lipoxygenase - Tarsius syrichta (Philippine tarsier) - ALOX15 gene & proteinALOX15 - arachidonate 15-lipoxygenase - Tarsius syrichta (Philippine tarsier) - ALOX15 gene & protein

PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. ... PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. ... arachidonate 15-lipoxygenaseImported. ,p>Information which has been imported from another database using automatic procedures ... tr,A0A1U7SWG9,A0A1U7SWG9_TARSY arachidonate 15-lipoxygenase OS=Tarsius syrichta GN=ALOX15 PE=3 SV=1 ...
more infohttp://www.uniprot.org/uniprot/A0A1U7SWG9

Arachidonate 15-lipoxygenase from human eosinophil-enriched leukocytes: partial purification and properties.  - PubMed - NCBIArachidonate 15-lipoxygenase from human eosinophil-enriched leukocytes: partial purification and properties. - PubMed - NCBI

Arachidonate 15-lipoxygenase was purified from human eosinophil-enriched leukocytes after showing that 15-lipoxygenase activity ... Arachidonate 15-lipoxygenase from human eosinophil-enriched leukocytes: partial purification and properties.. Sigal E1, ... In addition, ATP is shown to inhibit, and phosphatidylcholine is shown to stimulate, 15-lipoxygenase, suggesting a regulatory ... New evidence is presented suggesting that 15-lipoxygenase has electrostatic and hydrophobic properties distinct from 5- ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/3337718

ALOX12 (arachidonate 12-lipoxygenase) - KOMP (Knockout Mouse Project)ALOX12 (arachidonate 12-lipoxygenase) - KOMP (Knockout Mouse Project)

Vega: OTTMUSG6035 (Alox12, arachidonate 12-lipoxygenase)*CCDS: 24942, 24942.1*OMIM: ARACHIDONATE 12-OXIDOREDUCTASE; ALOX12*Gene ... arachidonate 12-lipoxygenase. Synonyms: 9930022G08Rik, Alox12p, P-12LO. Gene nomenclature, locus information, and GO, OMIM, and ...
more infohttps://www.komp.org/geneinfo.php?geneid=23143

JCI -
Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survivalJCI - Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival

Here, we determined that the gene encoding arachidonate 15-lipoxygenase (Alox15/15-LO) is essential for the survival of ... Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival. ... Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival. ...
more infohttps://www.jci.org/articles/view/66129/figure/4

JCI -
Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survivalJCI - Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival

Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival. Yaoyu Chen,1 Cong Peng,1 Sheela A. ... Alox15 encodes arachidonate 15-lipoxygenase (15-LO). Compared with Alox5, which we identified previously (19), Alox15 has ... Here, we determined that the gene encoding arachidonate 15-lipoxygenase (Alox15/15-LO) is essential for the survival of ... Inflammation and immune regulation by 12/15-lipoxygenases. Prog Lipid Res. 2006;45(4):334-356.. View this article via: PubMed ...
more infohttps://www.jci.org/articles/view/66129

Arachidonate 15-Lipoxygenase ELISA & Assay KitsArachidonate 15-Lipoxygenase ELISA & Assay Kits

Compare and order Arachidonate 15-Lipoxygenase ELISA Kits. View citations, images, detection ranges, sensitivity, prices and ... arachidonate omega-6 lipoxygenase , arachidonate 15-lipoxygenase , 12-LOX , arachidonate 12-lipoxygenase, leukocyte-type , 12/ ... arachidonate 12-lipoxygenase, 12S-type , Arachidonate 15-lipoxygenase , erythroid cell-specific 15-lipoxygenase , lipoxygenase ... Images for product: Arachidonate 15-Lipoxygenase (ALOX15) ELISA Kit Diagramm of the ELISA kit to detect Human 15-LOwith the ...
more infohttps://www.antikoerper-online.de/regulation-of-actin-filament-polymerization-pathway-56/alox15-elisa-kit-13142/

Arachidonate 15-Lipoxygenase ELISA & Assay KitsArachidonate 15-Lipoxygenase ELISA & Assay Kits

Compare and order Arachidonate 15-Lipoxygenase ELISA Kits. View citations, images, detection ranges, sensitivity, prices and ... arachidonate omega-6 lipoxygenase , arachidonate 15-lipoxygenase , 12-LOX , arachidonate 12-lipoxygenase, leukocyte-type , 12/ ... arachidonate 12-lipoxygenase, 12S-type , Arachidonate 15-lipoxygenase , erythroid cell-specific 15-lipoxygenase , lipoxygenase ... More product categories related to Arachidonate 15-Lipoxygenase ELISA Kit * 89 anti-Arachidonate 15-Lipoxygenase Primary ...
more infohttps://www.antibodies-online.com/regulation-of-actin-filament-polymerization-pathway-56/alox15-elisa-kit-13142/

ALOX15 (arachidonate 15-lipoxygenase) - KOMP (Knockout Mouse Project)ALOX15 (arachidonate 15-lipoxygenase) - KOMP (Knockout Mouse Project)

Vega: OTTMUSG6044 (Alox15, arachidonate 15-lipoxygenase)*CCDS: 24944, 24944.1*OMIM: ARACHIDONATE 15-LIPOXYGENASE; ALOX15*Gene ... arachidonate 15-lipoxygenase. Synonyms: 12-LO, Alox12l, L-12LO. Gene nomenclature, locus information, and GO, OMIM, and PMID ...
more infohttps://www.komp.org/geneinfo.php?MGI_Number=87997

Arachidonate 15-lipoxygenase type B knockdown leads to reduced lipid accumulation and inflammation in atherosclerosis.Arachidonate 15-lipoxygenase type B knockdown leads to reduced lipid accumulation and inflammation in atherosclerosis.

We have shown previously that arachidonate 15-lipoxygenase type B (ALOX15B) is more highly expressed in human atherosclerotic ... Arachidonate 15-Lipoxygenase / metabolism*. Atherosclerosis / complications, enzymology*, pathology. Cytokines / metabolism. ... Arachidonate 15-Lipoxygenase Type B Knockdown Leads to Reduced Lipid Accumulation and Inflammation in Atherosclerosis Alternate ... Arachidonate 15-lipoxygenase (ALOX15) catalyzes the production of eicosanoids that activate monocyte integrins and thereby ...
more infohttp://www.biomedsearch.com/nih/Arachidonate-15-lipoxygenase-type-B/22912809.html

Arachidonate 5-lipoxygenase elisa and antibodyArachidonate 5-lipoxygenase elisa and antibody

Recombinant Protein and Arachidonate 5-lipoxygenase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody ... Arachidonate 5-lipoxygenase. Arachidonate 5-lipoxygenase ELISA Kit. Arachidonate 5-lipoxygenase Recombinant. Arachidonate 5- ... lipoxygenase Antibody. Also known as Arachidonate 5-lipoxygenase (5-LO) (5-lipoxygenase).. Catalyzes the first step in ... Below are the list of possible Arachidonate 5-lipoxygenase products. If you cannot find the target and/or product is not ...
more infohttps://www.mybiosource.com/protein_family.php?root=arachidonate-5-lipoxygenase

OXER1, a G protein-coupled oxoeicosatetraenoid receptor, mediates the survival-promoting effects of arachidonate 5-lipoxygenase...OXER1, a G protein-coupled oxoeicosatetraenoid receptor, mediates the survival-promoting effects of arachidonate 5-lipoxygenase...

OXER1, a G protein-coupled oxoeicosatetraenoid receptor, mediates the survival-promoting effects of arachidonate 5-lipoxygenase ... OXER1, a G protein-coupled oxoeicosatetraenoid receptor, mediates the survival-promoting effects of arachidonate 5-lipoxygenase ... OXER1, a G protein-coupled oxoeicosatetraenoid receptor, mediates the survival-promoting effects of arachidonate 5-lipoxygenase ... OXER1, a G protein-coupled oxoeicosatetraenoid receptor, mediates the survival-promoting effects of arachidonate 5-lipoxygenase ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/23643940

Arachidonate 15-lipoxygenase - WikidataArachidonate 15-lipoxygenase - Wikidata

All structured data from the main and property namespace is available under the Creative Commons CC0 License; text in the other namespaces is available under the Creative Commons Attribution-ShareAlike License; additional terms may apply. By using this site, you agree to the Terms of Use and Privacy Policy. ...
more infohttps://www.wikidata.org/wiki/Q3621201

Arachidonate 5-lipoxygenase-activating proteinArachidonate 5-lipoxygenase-activating protein

Lipoxin and resolvin biosynthesis is dependent on 5-lipoxygenase activating protein.. 26159646. 2015. Variants in the ... Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5- ... Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and ... This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic ...
more infohttps://pharos.nih.gov/idg/targets/P20292

Arachidonate 12-lipoxygenase, 12R-typeArachidonate 12-lipoxygenase, 12R-type

The role of lipoxygenases in epidermis.. 23621129. 2013. Non-syndromic autosomal recessive congenital ichthyosis in the Israeli ... Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega- ... This review covers the background to discovery of the two key lipoxygenases (LOX) involved in epidermal barrier function, 12R- ... It was shown that 12R-lipoxygenase is involved in MUC5AC expression via ERK- and Sp1-signalling pathways.. ...
more infohttps://pharos.nih.gov/idg/targets/O75342

Rabbit 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit Manufacturers in DelhiRabbit 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit Manufacturers in Delhi

Arachidonate 5-Lipoxygenase) ELISA Kit OSCAR DIAGNOSTIC SERVICES PVT. LTD.is an India based Company in Delhi. ... Arachidonate 5-Lipoxygenase) ELISA Kit » Rabbit 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit. Rabbit 5-LO (Arachidonate 5- ... Lipoxygenase) ELISA Kit. Rabbit 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit. Rabbit 5-LO (Arachidonate 5-Lipoxygenase) ELISA ... Arachidonate 5-Lipoxygenase) ELISA Kit. Rabbit 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit. Rabbit 5-LO (Arachidonate 5- ...
more infohttps://www.odspl.com/sub-product/Rabbit+5-LO+%28Arachidonate+5-Lipoxygenase%29+ELISA+Kit.php

Arachidonate-5-Lipoxygenase (ALOX5): A Master Regulator for Prostate Cancer Cell SurvivalArachidonate-5-Lipoxygenase (ALOX5): A Master Regulator for Prostate Cancer Cell Survival

... Jagadananda Ghosh*. Departments of ... Central role of arachidonate 5-lipoxygenase in the regulation of cell growth and apoptosis in human prostate cancer cells. Adv ... Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells. Proc. Natl. Acad. Sci. USA ... Cite this article as: Ghosh J. Arachidonate-5-Lipoxygenase (ALOX5): A Master Regulator for Prostate Cancer Cell Survival. Clin ...
more infohttp://www.clinicsinoncology.com/full-text/cio-v2-id1277.php
  • Jobard F, Lefèvre C, Karaduman A, Blanchet-Bardon C, Emre S, Weissenbach J, Ozgüc M, Lathrop M, Prud'homme JF, Fischer J. Lipoxygenase-3 (ALOXE3) and 12(R)-lipoxygenase (ALOX12B) are mutated in non-bullous congenital ichthyosiform erythroderma (NCIE) linked to chromosome 17p13.1. (nih.gov)
  • It was shown that 12R-lipoxygenase is involved in MUC5AC expression via ERK- and Sp1-signalling pathways. (nih.gov)
  • Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega-hydroxyceramide for construction of the corneocyte lipid envelope. (nih.gov)
  • Lipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty acid hydroperoxidases. (genecards.org)
  • We have recently demonstrated that rat colon can convert 14 Carachidonic acid into both lipoxygenase and cyclo-oxygenase products, and that this metabolism is stimulated by the laxative phenolphthalein (Capasso et al 1984). (springer.com)
  • It was also found that the active 5-lipoxygenase metabolite, 5-oxoETE (a dehydrogenated derive of 5(S)-HETE), signals via its cognate receptor, called OXER1, which is a member of the seven-transmembrane G proteincoupled receptor (GPCR) family. (clinicsinoncology.com)
  • This gene encodes a member of the lipoxygenase family of proteins. (genecards.org)