Arachidonate lipoxygenases. Medical search

Enzymes catalyzing the oxidation of arachidonic acid to hydroperoxyarachidonates. These products are then rapidly converted by a peroxidase to hydroxyeicosatetraenoic acids. The positional specificity of the enzyme reaction varies from tissue to tissue. The final lipoxygenase pathway leads to the leukotrienes. EC 1.13.11.- .

Dioxygenases that catalyze the peroxidation of methylene-interrupted UNSATURATED FATTY ACIDS.

An enzyme of the oxidoreductase class primarily found in PLANTS. It catalyzes reactions between linoleate and other fatty acids and oxygen to form hydroperoxy-fatty acid derivatives.

An enzyme that catalyzes the oxidation of arachidonic acid to yield 12-hydroperoxyarachidonate (12-HPETE) which is itself rapidly converted by a peroxidase to 12-hydroxy-5,8,10,14-eicosatetraenoate (12-HETE). The 12-hydroperoxides are preferentially formed in PLATELETS.

An enzyme that catalyzes the oxidation of arachidonic acid to yield 15-hydroperoxyarachidonate (15-HPETE) which is rapidly converted to 15-hydroxy-5,8,11,13-eicosatetraenoate (15-HETE). The 15-hydroperoxides are preferentially formed in NEUTROPHILS and LYMPHOCYTES.

An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.

An enzyme that catalyzes the oxidation of arachidonic acid to yield 5-hydroperoxyarachidonate (5-HPETE) which is rapidly converted by a peroxidase to 5-hydroxy-6,8,11,14-eicosatetraenoate (5-HETE). The 5-hydroperoxides are preferentially formed in leukocytes.

Compounds that bind to and inhibit that enzymatic activity of LIPOXYGENASES. Included under this category are inhibitors that are specific for lipoxygenase subtypes and act to reduce the production of LEUKOTRIENES.

A lipoxygenase metabolite of ARACHIDONIC ACID. It is a highly selective ligand used to label mu-opioid receptors in both membranes and tissue sections. The 12-S-HETE analog has been reported to augment tumor cell metastatic potential through activation of protein kinase C. (J Pharmacol Exp Ther 1995; 274(3):1545-51; J Natl Cancer Inst 1994; 86(15):1145-51)

Eighteen-carbon essential fatty acids that contain two double bonds.

A potent lipoxygenase inhibitor that interferes with arachidonic acid metabolism. The compound also inhibits formyltetrahydrofolate synthetase, carboxylesterase, and cyclooxygenase to a lesser extent. It also serves as an antioxidant in fats and oils.

Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.

A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.

Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes.

An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.

A class of compounds named after and generally derived from C20 fatty acids (EICOSANOIC ACIDS) that includes PROSTAGLANDINS; LEUKOTRIENES; THROMBOXANES, and HYDROXYEICOSATETRAENOIC ACIDS. They have hormone-like effects mediated by specialized receptors (RECEPTORS, EICOSANOID).

A doubly unsaturated fatty acid, occurring widely in plant glycosides. It is an essential fatty acid in mammalian nutrition and is used in the biosynthesis of prostaglandins and cell membranes. (From Stedman, 26th ed)

A 20-carbon unsaturated fatty acid containing 4 alkyne bonds. It inhibits the enzymatic conversion of arachidonic acid to prostaglandins E(2) and F(2a).

Enzyme complexes that catalyze the formation of PROSTAGLANDINS from the appropriate unsaturated FATTY ACIDS, molecular OXYGEN, and a reduced acceptor.

FATTY ACIDS in which the carbon chain contains one or more double or triple carbon-carbon bonds.

A plant genus of the family BETULACEAE known for the edible nuts.

Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.

A dual inhibitor of both cyclooxygenase and lipoxygenase pathways. It exerts an anti-inflammatory effect by inhibiting the formation of prostaglandins and leukotrienes. The drug also enhances pulmonary hypoxic vasoconstriction and has a protective effect after myocardial ischemia.

Trihydroxy derivatives of eicosanoic acids. They are primarily derived from arachidonic acid, however eicosapentaenoic acid derivatives also exist. Many of them are naturally occurring mediators of immune regulation.

Phospholipases that hydrolyze one of the acyl groups of phosphoglycerides or glycerophosphatidates.

20-carbon saturated monocarboxylic acids.

Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.

Phospholipases that hydrolyze the acyl group attached to the 2-position of PHOSPHOGLYCERIDES.

Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)

An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.

Compounds or agents that combine with cyclooxygenase (PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES) and thereby prevent its substrate-enzyme combination with arachidonic acid and the formation of eicosanoids, prostaglandins, and thromboxanes.

A plant species of the genus SOLANUM, family SOLANACEAE. The starchy roots are used as food. SOLANINE is found in green parts.

The rate dynamics in chemical or physical systems.

Eighteen-carbon essential fatty acids that contain three double bonds.

Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.

A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.

A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.

A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).

Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.

(11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.

A constitutively-expressed subtype of prostaglandin-endoperoxide synthase. It plays an important role in many cellular processes.

A stable, physiologically active compound formed in vivo from the prostaglandin endoperoxides. It is important in the platelet-release reaction (release of ADP and serotonin).

Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.

The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.

A non-steroidal anti-inflammatory agent (NSAID) that inhibits the enzyme cyclooxygenase necessary for the formation of prostaglandins and other autacoids. It also inhibits the motility of polymorphonuclear leukocytes.

A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.

An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.

The major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990)

Eighteen-carbon cyclopentyl polyunsaturated fatty acids derived from ALPHA-LINOLENIC ACID via an oxidative pathway analogous to the EICOSANOIDS in animals. Biosynthesis is inhibited by SALICYLATES. A key member, jasmonic acid of PLANTS, plays a similar role to ARACHIDONIC ACID in animals.

Long chain organic acid molecules that must be obtained from the diet. Examples are LINOLEIC ACIDS and LINOLENIC ACIDS.

A class of enzymes that catalyze the hydrolysis of phosphoglycerides or glycerophosphatidates. EC 3.1.-.

Leukotriene A synthase activity of purified mouse skin arachidonate 8-lipoxygenase expressed in Escherichia coli. (1/145)

Mouse skin 8-lipoxygenase was expressed in COS-7 cells by transient transfection of its cDNA in pEF-BOS carrying an elongation factor-1alpha promoter. When crude extract of the transfected COS-7 cells was incubated with arachidonic acid, 8-hydroxy-5,9,11, 14-eicosatetraenoic acid was produced as assessed by reverse- and straight-phase high performance liquid chromatographies. The recombinant enzyme also reacted on alpha-linolenic and docosahexaenoic acids at almost the same rate as that with arachidonic acid. Eicosapentaenoic and gamma-linolenic acids were also oxygenated at 43% and 56% reaction rates of arachidonic acid, respectively. In contrast, linoleic acid was a poor substrate for this enzyme. The 8-lipoxygenase reaction with these fatty acids proceeded almost linearly for 40 min. The 8-lipoxygenase was also expressed in an Escherichia coli system using pQE-32 carrying six histidine residues at N-terminal of the enzyme. The expressed enzyme was purified over 380-fold giving a specific activity of approximately 0.2 micromol/45 min per mg protein by nickel-nitrilotriacetate affinity chromatography. The enzymatic properties of the purified 8-lipoxygenase were essentially the same as those of the enzyme expressed in COS-7 cells. When the purified 8-lipoxygenase was incubated with 5-hydroperoxy-6,8,11, 14-eicosatetraenoic acid, two epimers of 6-trans-leukotriene B4, degradation products of unstable leukotriene A4, were observed upon high performance liquid chromatography. Thus, the 8-lipoxygenase catalyzed synthesis of leukotriene A4 from 5-hydroperoxy fatty acid. Reaction rate of the leukotriene A synthase was approximately 7% of arachidonate 8-lipoxygenation. In contrast to the linear time course of 8-lipoxygenase reaction with arachidonic acid, leukotriene A synthase activity leveled off within 10 min, indicating suicide inactivation. (+info)

Identification of amino acid determinants of the positional specificity of mouse 8S-lipoxygenase and human 15S-lipoxygenase-2. (2/145)

Phorbol ester-inducible mouse 8S-lipoxygenase (8-LOX) and its human homologue, 15S-lipoxygenase-2 (15-LOX-2), share 78% identity in amino acid sequences, yet there is no overlap in their positional specificities. In this study, we investigated the determinants of positional specificity using a random chimeragenesis approach in combination with site-directed mutagenesis. Exchange of the C-terminal one-third of the 8-LOX with the corresponding portion of 15-LOX-2 yielded a chimeric enzyme with exclusively 15S-lipoxygenase activity. The critical region was narrowed down to a cluster of five amino acids by expression of multiple cDNAs obtained by in situ chimeragenesis in Escherichia coli. Finally, a pair of amino acids, Tyr(603) and His(604), was identified as the positional determinant by site-directed mutagenesis. Mutation of both of these amino acids to the corresponding amino acids in 15-LOX-2 (Asp and Val, respectively) converted the positional specificity from 8S to 90% 15S without yielding any other by-products. Mutation of the corresponding residues in 15-LOX-2 to the 8-LOX sequence changed specificity to 50% oxygenation at C-8 for one amino acid substitution and 70% at C-8 for the double mutant. Based on the crystal structure of the reticulocyte 15-LOX, these two amino acids lie opposite the open coordination position of the catalytic iron in a likely site for substrate binding. The change from 8 to 15 specificity entails a switch in the head to tail binding of substrate. Enzymes that react with substrate "head first" (5-LOX and 8-LOX) have a bulky aromatic amino acid and a histidine in these positions, whereas lipoxygenases that accept substrates "tail first" (12-LOX and 15-LOX) have an aliphatic residue with a glutamine or aspartate. Thus, this positional determinant of the 8-LOX and 15-LOX-2 may have significance for other lipoxygenases. (+info)

8S-lipoxygenase products activate peroxisome proliferator-activated receptor alpha and induce differentiation in murine keratinocytes. (3/145)

To determine the function and mechanism of action of the 8S-lipoxygenase (8-LOX) product of arachidonic acid, 8S-hydroxyeicosatetraenoic acid (8S-HETE), which is normally synthesized only after irritation of the epidermis, transgenic mice with 8-LOX targeted to keratinocytes through the use of a loricrin promoter were generated. Histological analyses showed that the skin, tongue, and stomach of transgenic mice are highly differentiated, and immunoblotting and immunohistochemistries of skin showed higher levels of keratin-1 expression compared with wild-type mice. The labeling index, however, of the transgenic epidermis was twice that of the wild-type epidermis. Furthermore, 8S-HETE treatment of wild-type primary keratinocytes induced keratin-1 expression. Peroxisome proliferator activated receptor alpha (PPARalpha) was identified as a crucial component of keratin-1 induction through transient transfection with expression vectors for PPARalpha, PPARgamma, and a dominant-negative PPAR, as well as through the use of known PPAR agonists. From these studies, it is concluded that 8S-HETE plays an important role in keratinocyte differentiation and that at least some of its effects are mediated by PPARalpha. (+info)

A pertussis toxin-sensitive 8-lipoxygenase pathway is activated by a nicotinic acetylcholine receptor in aplysia neurons. (4/145)

Acetylcholine (ACh) activates two types of chloride conductances in Aplysia neurons that can be distinguished by their kinetics and pharmacology. One is a rapidly desensitizing current that is blocked by alpha-conotoxin-ImI and the other is a sustained current that is insensitive to the toxin. These currents are differentially expressed in Aplysia neurons. We report here that neurons that respond to ACh with a sustained chloride conductance also generate 8-lipoxygenase metabolites. The sustained chloride conductance and the activation of 8-lipoxygenase have similar pharmacological profiles. Both are stimulated by suberyldicholine and nicotine, and both are inhibited by alpha-bungarotoxin. Like the sustained chloride conductance, the activation of 8-lipoxygenase is not blocked by alpha-conotoxin-ImI. In spite of the similarities between the metabolic and electrophysiological responses, the generation of 8-lipoxygenase metabolites does not appear to depend on the ion current since an influx of chloride ions is neither necessary nor sufficient for the formation of the lipid metabolites. In addition, the application of pertussis toxin blocked the ACh-activated release of arachidonic acid and the subsequent production of 8-lipoxygenase metabolites, yet the ACh-induced activation of the chloride conductance is not dependent on a G protein. Our results are consistent with the idea that the nicotinic ACh receptor that activates the sustained chloride conductance can, independent of the chloride ion influx, initiate lipid messenger synthesis. (+info)

Elevated expression of 12/15-lipoxygenase and cyclooxygenase-2 in a transgenic mouse model of prostate carcinoma. (5/145)

Changes in expression of arachidonic acid (AA) metabolizing enzymes are implicated in the development and progression of human prostate carcinoma (Pca). Transgenic mouse models of Pca that progress from high-grade prostatic intraepithelial neoplasia (HGPIN) to invasive and metastatic carcinoma could facilitate study of the regulation and function of these genes in Pca progression. Herein we characterize the AA-metabolizing enzymes in transgenic mice established with a prostate epithelial-specific long probasin promoter and the SV40 large T antigen (LPB-Tag mice) that develop extensive HGPIN and invasive and metastatic carcinoma with neuroendocrine (NE) differentiation. Murine 8-lipoxygenase (8-LOX), homologue of the 15-LOX-2 enzyme that is expressed in benign human prostatic epithelium and reduced in Pca, was not detected in wild-type or LPB-Tag prostates as determined by enzyme assay, reverse transcription-PCR, and immunohistochemistry. The most prominent AA metabolite in mouse prostate was 12-HETE. Wild-type prostate (dorsolateral lobe) converted 1.6 +/- 0.5% [(14)C]AA to 12-HETE (n = 7), and this increased to 8.0 +/- 4.4% conversion in LPB-Tag mice with HGPIN (n = 13). Quantitative real-time reverse transcription-PCR and immunostaining correlated the increased 12-HETE synthesis with increased neoplastic epithelial expression of 12/15-LOX, the leukocyte-type (L) of 12-LOX and the murine homologue of human 15-LOX-1. Immunostaining showed increased L12-LOX in invasive carcinoma and approximately one-half of metastatic foci. COX-2 mRNA was detectable in neoplastic prostates with HGPIN but not in wild-type prostate. By immunostaining, COX-2 was increased in the neoplastic epithelium of HGPIN but was absent in foci of invasion and metastases. We conclude that (a) AA metabolism in wild-type mouse prostate differs from humans in the basal expression of LOXs (15-LOX-2 in human, absence of its 8-LOX homologue in mouse prostate); (b) increased expression of 12/15-LOX in HGPIN and invasive carcinoma of the LPB-Tag model is similar to the increased 15-LOX-1 in high-grade human Pca; and (c) the LPB-Tag model shows increased COX-2 in HGPIN, and therefore, it may allow additional definition of the role of this enzyme in the subset of human HGPINs or other precursor lesions that are COX-2 positive, as well as investigation of its contribution to neoplastic cell proliferation and tumor angiogenesis in Pca. (+info)

Upregulation of 8-lipoxygenase in the dermatitis of IkappaB-alpha-deficient mice. (6/145)

Neonatal mice deficient in IkappaB-alpha, an inhibitor of the ubiquitous transcription factor NF-kappaB, develop severe and widespread dermatitis shortly after birth. In humans, inflammatory skin disorders such as psoriasis are associated with accumulation in the skin of the unusual arachidonic acid metabolite 12R-hydroxyeicosatetraenoic acid (12R-HETE), a product of the enzyme 12R-lipoxygenase. To examine the etiology of the murine IkappaB-alpha-deficient skin phenotype, we investigated the expression of lipoxygenases and the metabolism of exogenous arachidonic acid in the skin. In the IkappaB-alpha-deficient animals, the major lipoxygenase metabolite was 8S-HETE, formed together with a minor amount of 12S-HETE; 12R-HETE synthesis was undetectable. Skin from the wild-type littermates formed 12S-HETE as the almost exclusive lipoxygenase metabolite. Upregulation of 8S-lipoxygenase (8-LOX) in IkappaB-alpha-deficient mice was confirmed at the transcriptional and translational level using ribonuclease protection assay and western analysis. In immunohistochemical studies, increased expression of 8-LOX was detected in the stratum granulosum of the epidermis. In the stratum granulosum, 8-LOX may be involved in the terminal differentiation of keratinocytes. Although mouse 8S-lipoxygenase and human 12R-lipoxygenase are not ortholog genes, we speculate that in mouse and humans the two different enzymes may fulfill equivalent functions in the progression of inflammatory dermatoses. (+info)

Arachidonate lipoxygenase (ALOX) and cyclooxygenase (COX) polymorphisms and colon cancer risk. (7/145)

In the human colon, arachidonic acid is metabolized primarily by cyclooxygenase (COX) and arachidonate lipoxygenase (ALOX) to bioactive lipids, which are implicated in colon cancer risk. Several polymorphisms in ALOX and COX genes have been identified, including G-1752A, G-1699A and Glu254Lys in ALOX5; Gln261Arg in ALOX12; Leu237Met and Val481Ile in COX1; and C-645T and Val511Ala in COX2. Because of the significant role of arachidonic acid metabolism in colon cancer, we hypothesized that these polymorphisms could influence susceptibility to colon cancer. We addressed this hypothesis in African-Americans and Caucasians using colon cancer cases (n = 293) and hospital- (n = 229) and population-based (n = 304) control groups. Polymorphisms did not differ between the control groups (P > 0.05); thus, they are combined for all analyses presented. ALOX5 Glu254Lys and COX2 C-645T and Val511Ala allele frequencies differed between Caucasians and African-American controls (P < 0.001). The ALOX5 -1752 and -1699 polymorphisms were in linkage disequilibrium (P < 0.001) and associated with a decreased risk in Caucasians in ALOX5 haplotype analyses (P = 0.03). Furthermore, an inverse association was observed between A alleles at positions -1752 and -1699 of ALOX5 and colon cancer risk in Caucasians, but not in African-Americans. Caucasians with A alleles at ALOX5 -1752 had a reduced odds of colon cancer versus those with G alleles [odds ratio (OR) (GA versus GG), 0.63; 95% confidence interval (CI), 0.39-1.01; OR (AA versus GG), 0.33; 95% CI, 0.07-1.65, P(trend) = 0.02]. Similar results were observed for ALOX5 G-1699A [OR (GA versus GG), 0.59, 95% CI, 0.37-0.94; OR (AA versus GG), 0.27, 95% CI, 0.06-1.32, P(trend) = 0.01]. Statistically significant associations with colon cancer were not observed for the other polymorphisms investigated. We have shown for the first time that a haplotype containing ALOX5 G-1752A and G-1699A in a negative regulatory region of the promoter may influence colon cancer risk in Caucasians. (+info)

Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality. (8/145)

Lipoxygenases (LOXs) catalyze the regio- and stereospecific dioxygenation of polyunsaturated membrane-embedded fatty acids. We report here the 3.2 A resolution structure of 8R-LOX from the Caribbean sea whip coral Plexaura homomalla, a LOX isozyme with calcium dependence and the uncommon R chiral stereospecificity. Structural and spectroscopic analyses demonstrated calcium binding in a C2-like membrane-binding domain, illuminating the function of similar amino acids in calcium-activated mammalian 5-LOX, the key enzyme in the pathway to the pro-inflammatory leukotrienes. Mutation of Ca(2+)-ligating amino acids in 8R-LOX resulted not only in a diminished capacity to bind membranes, as monitored by fluorescence resonance energy transfer, but also in an associated loss of Ca(2+)-regulated enzyme activity. Moreover, a structural basis for R chiral specificity is also revealed; creation of a small oxygen pocket next to Gly(428) (Ala in all S-LOX isozymes) promoted C-8 oxygenation with R chirality on the activated fatty acid substrate. (+info)

Arachidonate 5-lipoxygenase, also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13 ... that in humans is encoded by the ALOX5 gene.[5] Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. ... "Alox5 - arachidonate 5-lipoxygenase". WikiGenes.. *^ Fahel JS, de Souza MB, Gomes MT, Corsetti PP, Carvalho NB, Marinho FA, de ... arachidonate 5-lipoxygenase activity. • dioxygenase activity. • metal ion binding. • protein binding. • oxidoreductase activity ...

https://en.wikipedia.org/wiki/Arachidonate_5-lipoxygenase

Arachidonate 8-lipoxygenase (EC 1.13.11.40) is an enzyme that catalyzes the chemical reaction arachidonate + O2 ⇌ {\ ... Other names in common use include 8-lipoxygenase, and 8(R)-lipoxygenase. This enzyme participates in arachidonic acid ... Bundy GL, Nidy EG, Epps DE, Mizsak SA, Wnuk RJ (1986). "Discovery of an arachidonic acid C-8 lipoxygenase in the gorgonian ... The systematic name of this enzyme class is arachidonate:oxygen 8-oxidoreductase. ...

https://en.wikipedia.org/wiki/Arachidonate_8-lipoxygenase

Arachidonate + O2 = 5Z,8Z,11Z,13E-15S-15-hydroperoxyicosa-5,8,11,13-tetraenoate. UniProt ...

http://www.rcsb.org/pdb/protein/P27479

The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.

http://www.rcsb.org/pdb/protein/E5FPY8

Arachidonate 15-lipoxygenase was purified from human eosinophil-enriched leukocytes after showing that 15-lipoxygenase activity ... Arachidonate 15-lipoxygenase from human eosinophil-enriched leukocytes: partial purification and properties.. Sigal E1, ... In addition, ATP is shown to inhibit, and phosphatidylcholine is shown to stimulate, 15-lipoxygenase, suggesting a regulatory ... New evidence is presented suggesting that 15-lipoxygenase has electrostatic and hydrophobic properties distinct from 5- ...

https://www.ncbi.nlm.nih.gov/pubmed/3337718

Here, we determined that the gene encoding arachidonate 15-lipoxygenase (Alox15/15-LO) is essential for the survival of ... Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival. ... Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival. ...

https://www.jci.org/articles/view/66129/figure/8

Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival. Yaoyu Chen,1 Cong Peng,1 Sheela A. ... Alox15 encodes arachidonate 15-lipoxygenase (15-LO). Compared with Alox5, which we identified previously (19), Alox15 has ... Here, we determined that the gene encoding arachidonate 15-lipoxygenase (Alox15/15-LO) is essential for the survival of ... Inflammation and immune regulation by 12/15-lipoxygenases. Prog Lipid Res. 2006;45(4):334-356.. View this article via: PubMed ...

https://www.jci.org/articles/view/66129

12S-lipoxygenase. Arachidonate 12-lipoxygenase, 12S-type. arachidonate (12S)-lipoxygenase. arachidonate (15S)-lipoxygenase. ... ALOX12 arachidonate 12-lipoxygenase, 12S type [Homo sapiens] ALOX12 arachidonate 12-lipoxygenase, 12S type [Homo sapiens]. Gene ... arachidonate 15-lipoxygenase,15S-type. linoleate 13S-lipoxygenase. lipoxin synthase 12-LO. platelet 12-LOX. platelet-type 12- ... arachidonate 12-lipoxygenase, 12S typeprovided by HGNC. Primary source. HGNC:HGNC:429 See related. Ensembl:ENSG00000108839 MIM: ...

https://www.ncbi.nlm.nih.gov/gene/239

PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. ... PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. ... arachidonate 15-lipoxygenaseImported. ,p>Information which has been imported from another database using automatic procedures ... tr,A0A1U7SWG9,A0A1U7SWG9_TARSY arachidonate 15-lipoxygenase OS=Tarsius syrichta GN=ALOX15 PE=3 SV=1 ...

http://www.uniprot.org/uniprot/A0A1U7SWG9

We have recently demonstrated that rat colon can convert 14Carachidonic acid into both lipoxygenase and cyclo-oxygenase ... Capasso F., Tavares I.A., Bennett A. (1985) Stimulated Human Colonic Synthesis of Arachidonate Lipoxygenase and Cyclo-Oxygenase ... Stimulated Human Colonic Synthesis of Arachidonate Lipoxygenase and Cyclo-Oxygenase Products by Laxatives. ... Arachidonic Acid Ricinoleic Acid Arachidonic Acid Metabolism Lipoxygenase Product Sodium Sulfosuccinate These keywords were ...

https://link.springer.com/chapter/10.1007%2F978-1-4684-7841-9_28

The worlds first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.

https://www.wikigenes.org/e/gene/e/567204.html

Arachidonate 12-Lipoxygenase) Homo sapiens, Authors: Sreeparna Banerjee, Asli Erdog. Published in: Atlas Genet Cytogenet Oncol ... ALOX12 17p13.1 arachidonate 12-lipoxygenase (Homo sapiens), ALOX12P2 17p13 arachidonate 12-lipoxygenase pseudogene 2, TEKT1 ... arachidonate 12-lipoxygenase activity iron ion binding protein binding cytoplasm cytosol cytosol aging positive regulation of ... arachidonate 12-lipoxygenase activity iron ion binding protein binding cytoplasm cytosol cytosol aging positive regulation of ...

http://atlasgeneticsoncology.org/Genes/ALOX12ID620ch17p13.html

Compare and order Arachidonate 15-Lipoxygenase ELISA Kits. View citations, images, detection ranges, sensitivity, prices and ... arachidonate omega-6 lipoxygenase , arachidonate 15-lipoxygenase , 12-LOX , arachidonate 12-lipoxygenase, leukocyte-type , 12/ ... arachidonate 12-lipoxygenase, 12S-type , Arachidonate 15-lipoxygenase , erythroid cell-specific 15-lipoxygenase , lipoxygenase ... Images for product: Arachidonate 15-Lipoxygenase (ALOX15) ELISA Kit Diagramm of the ELISA kit to detect Human 15-LOwith the ...

https://www.antikoerper-online.de/regulation-of-actin-filament-polymerization-pathway-56/alox15-elisa-kit-13142/

Compare and order Arachidonate 15-Lipoxygenase ELISA Kits. View citations, images, detection ranges, sensitivity, prices and ... arachidonate omega-6 lipoxygenase , arachidonate 15-lipoxygenase , 12-LOX , arachidonate 12-lipoxygenase, leukocyte-type , 12/ ... arachidonate 12-lipoxygenase, 12S-type , Arachidonate 15-lipoxygenase , erythroid cell-specific 15-lipoxygenase , lipoxygenase ... More product categories related to Arachidonate 15-Lipoxygenase ELISA Kit * 89 anti-Arachidonate 15-Lipoxygenase Primary ...

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We have shown previously that arachidonate 15-lipoxygenase type B (ALOX15B) is more highly expressed in human atherosclerotic ... Arachidonate 15-Lipoxygenase / metabolism*. Atherosclerosis / complications, enzymology*, pathology. Cytokines / metabolism. ... Arachidonate 15-Lipoxygenase Type B Knockdown Leads to Reduced Lipid Accumulation and Inflammation in Atherosclerosis Alternate ... Arachidonate 15-lipoxygenase (ALOX15) catalyzes the production of eicosanoids that activate monocyte integrins and thereby ...

http://www.biomedsearch.com/nih/Arachidonate-15-lipoxygenase-type-B/22912809.html

Vega: OTTMUSG6035 (Alox12, arachidonate 12-lipoxygenase)*CCDS: 24942, 24942.1*OMIM: ARACHIDONATE 12-OXIDOREDUCTASE; ALOX12*Gene ... arachidonate 12-lipoxygenase. Synonyms: 9930022G08Rik, Alox12p, P-12LO. Gene nomenclature, locus information, and GO, OMIM, and ...

https://www.komp.org/geneinfo.php?geneid=23143

Vega: OTTMUSG6044 (Alox15, arachidonate 15-lipoxygenase)*CCDS: 24944, 24944.1*OMIM: ARACHIDONATE 15-LIPOXYGENASE; ALOX15*Gene ... arachidonate 15-lipoxygenase. Synonyms: 12-LO, Alox12l, L-12LO. Gene nomenclature, locus information, and GO, OMIM, and PMID ...

https://www.komp.org/geneinfo.php?MGI_Number=87997

Publications] T.Arakawa: The transcriptional regulation of human arachidonate 12-lipoxygenase gene by NFκB/Rel FEBS Lett.363 ... Publications] T.Arakawa: The transcriptional regulation of human arachidonate 12-lipoxygenase gene by NFkB/Rel FEBS Lett.363 ... 15-Lipoxygenase is now under investigations.. 3) 12-Lipoxygenase of human uterine cervix : Nigams group of Free University of ... Publications] M.Nakamura: Immunocytochemical localization of platelet-type arachidonate 12-lipoxygenase in mouse blood cells ...

https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044273/

It inhibits synthesis of prostaglandins and leukotrienes by inhibition in the cyclooxygenase and lipoxygenase pathways of... ... Effects of the Arachidonate Lipoxygenase Inhibitor BW755C on Traumatic and Peritumoural Brain Oedema. ... Higgs GA, Mugridge KG, Moncada S, Vane JR (1984) Inhibition of tissue damage by the arachidonate lipoxygenase inhibitor BW755C ... Ikeda Y., Long D.M. (1990) Effects of the Arachidonate Lipoxygenase Inhibitor BW755C on Traumatic and Peritumoural Brain Oedema ...

https://rd.springer.com/chapter/10.1007/978-3-7091-9115-6_23

Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5- ... lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this ... with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been ... arachidonate 5-lipoxygenase-activating protein. Background. This gene encodes a protein which, with 5-lipoxygenase, is required ...

https://www.creativebiomart.net/symbolsearch_alox5ap.htm

Recombinant Protein and Arachidonate 5-lipoxygenase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody ... Arachidonate 5-lipoxygenase. Arachidonate 5-lipoxygenase ELISA Kit. Arachidonate 5-lipoxygenase Recombinant. Arachidonate 5- ... lipoxygenase Antibody. Also known as Arachidonate 5-lipoxygenase (5-LO) (5-lipoxygenase).. Catalyzes the first step in ... Below are the list of possible Arachidonate 5-lipoxygenase products. If you cannot find the target and/or product is not ...

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Arachidonate 5-Lipoxygenase, Arachidonic Acid 5-Lipoxygen, item number: L2623-10C.100 from United States Biological at Biomol! ... Anti-Lipoxygenase, 5- (5-LOX, 5-LO, 5-LPG, Arachidonate 5-Lipoxygenase, Arachidonic Acid 5-Lipoxygen ... Product information "Anti-Lipoxygenase, 5- (5-LOX, 5-LO, 5-LPG, Arachidonate 5-Lipoxygenase, Arachidonic Acid 5-Lipoxygen" ... Customer review for "Anti-Lipoxygenase, 5- (5-LOX, 5-LO, 5-LPG, Arachidonate 5-Lipoxygenase, Arachidonic Acid 5-Lipoxygen" ...

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Protein kinase C regulates 15-lipoxygenase oxidation of membrane-bound arachidonate in human monocytes ... Protein kinase C regulates 15-lipoxygenase oxidation of membrane-bound arachidonate in human monocytes In: 10th Annual Meeting ...

http://epubs.surrey.ac.uk/824040/

SpecificityC TerminusStorage/StabilityAliquot and store at -20°C Minimize freezing and thawing More InformationImmunogenThe immunogen was a 16-residue peptide matching a sequence from the C Terminus of Human Rat ALOX5 See Accession Number s NP_000689 1 Formulation 0 5 mg/ml in TBS 0 02%

https://www.raybiotech.com/goat-anti-human-rat-arachidonate-5-lipoxygenase-c-terminus/

All structured data from the main and property namespace is available under the Creative Commons CC0 License; text in the other namespaces is available under the Creative Commons Attribution-ShareAlike License; additional terms may apply. By using this site, you agree to the Terms of Use and Privacy Policy. ...

https://www.wikidata.org/wiki/Q3621201

Lipoxin and resolvin biosynthesis is dependent on 5-lipoxygenase activating protein.. 26159646. 2015. Variants in the ... Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5- ... Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and ... This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic ...

https://pharos.nih.gov/idg/targets/P20292

The role of lipoxygenases in epidermis.. 23621129. 2013. Non-syndromic autosomal recessive congenital ichthyosis in the Israeli ... Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega- ... This review covers the background to discovery of the two key lipoxygenases (LOX) involved in epidermal barrier function, 12R- ... It was shown that 12R-lipoxygenase is involved in MUC5AC expression via ERK- and Sp1-signalling pathways.. ...

https://pharos.nih.gov/idg/targets/O75342

Human ALOX15B(Arachidonate 15-lipoxygenase B) ELISA Kit. $430.00. Human ALOX15B(Arachidonate 15-lipoxygenase B) ELISA Kit ... Home / Quantitative Biomarker Assays / Human / Human ALOX15B(Arachidonate 15-lipoxygenase B) ELISA Kit. ...

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Arachidonate 5-Lipoxygenase) ELISA Kit OSCAR DIAGNOSTIC SERVICES PVT. LTD.is an India based Company in Delhi. ... Arachidonate 5-Lipoxygenase) ELISA Kit » Rabbit 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit. Rabbit 5-LO (Arachidonate 5- ... Lipoxygenase) ELISA Kit. Rabbit 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit. Rabbit 5-LO (Arachidonate 5-Lipoxygenase) ELISA ... Arachidonate 5-Lipoxygenase) ELISA Kit. Rabbit 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit. Rabbit 5-LO (Arachidonate 5- ...

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Here, we determined that the gene encoding arachidonate 15-lipoxygenase (Alox15/15-LO) is essential for the survival of leukemia stem cells (LSCs) in a murine model of BCR-ABL-induced chronic myeloid leukemia (CML). (jci.org)
According to NCBI Map Viewer, genes flanking ALOX15 in centromere to telomere direction on 17p13 are: GABARAP 17p13.1 GABA(A) receptor-associated protein, ASGR2 asialoglycoprotein receptor 2, ALOX12 17p13.1 arachidonate 12-lipoxygenase (Homo sapiens), ALOX12P2 17p13 arachidonate 12-lipoxygenase pseudogene 2, TEKT1 tektin 1, FBXO39 F-box protein 39. (atlasgeneticsoncology.org)
ALOX15 (Arachidonate 15-Lipoxygenase) is a Protein Coding gene. (genecards.org)
One of the key enzymes involved in the metabolism of polyunsaturated fatty acids to 4HNE in somatic cells is arachidonate 15-lipoxygenase (ALOX15). (edu.au)

Arachidonate 5-lipoxygenase , also known as ALOX5 , 5-lipoxygenase , 5-LOX , or 5-LO , is a non- heme iron-containing enzyme (EC 1.13.11.34) that in humans is encoded by the ALOX5 gene . (wikipedia.org)
The ALOX5 gene, which occupies 71.9 kilo base pairs (kb) on chromosome 10 (all other human lipoxygenases are clustered together on chromosome 17), is composed of 14 exons divided by 13 introns encoding the mature 78 kilodalton (kD) ALOX5 protein consisting of 673 amino acids. (wikipedia.org)
Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). (nih.gov)
Ghosh J. Arachidonate-5-Lipoxygenase (ALOX5): A Master Regulator for Prostate Cancer Cell Survival. (clinicsinoncology.com)
Orthologous to human ALOX5 (arachidonate 5-lipoxygenase). (jax.org)

According to the NCBI conserved domain search, the presence of a polycystin/lipoxygenase/alpha-toxin (PLAT) domain in the 12-LOX protein allows it access and enables it to catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and plasma lipoproteins. (atlasgeneticsoncology.org)
Several lines of evidence suggested that the 14,15-epoxy acid was covalently incorporated into the enzyme protein and the leukocyte 12-lipoxygenase was suicide-inactivated. (nii.ac.jp)
This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. (creativebiomart.net)
Bermudez, AF , Coles, B , Coffey, M and O'Donnell, V (2003) Protein kinase C regulates 15-lipoxygenase oxidation of membrane-bound arachidonate in human monocytes In: 10th Annual Meeting of the Society-for-Free-Radical-Biology-and-Medicne, 2003-11-20 - 2003-11-24, SEATTLE, WASHINGTON. (surrey.ac.uk)
ALOX5AP (Arachidonate 5-Lipoxygenase Activating Protein) is a Protein Coding gene. (genecards.org)
Epidermal growth factor (EGF), determined by immunoprecipitation and Western blot analysis, increased both enzyme activity and protein level of 12-lipoxygenase in the solubilized microsomes of human epidermoid carcinoma A431 cells, respectively. (elsevier.com)
The time-dependent induction of luciferase activity by EGF paralleled the EGF-induced enzyme activity and expression of 12-lipoxygenase protein. (elsevier.com)
The arachidonate 5-lipoxygenase activating protein gene polymorphism is associated with the risk of scleroderma-related interstitial lung disease: a multicentre European Scleroderma Trials and Research group (EUSTAR) study. (cdc.gov)
The arachidonate 5-lipoxygenase activating protein (ALOX5AP) regulates synthesis of leukotrienes (LTs), which are important mediators of inflammation and connective tissue remodelling. (cdc.gov)
Recombinant full length protein corresponding to Human 15 Lipoxygenase 1. (abcam.com)
1993) 5‐Lipoxygenase‐activating protein stimulates the utilization of arachidonic acid by 5‐lipoxygenase. (els.net)

In humans, Arachidonate 12-lipoxygenase (12-LO, 12-LOX, ALO12, or platelet type 12-lipoxygenase) is encoded by the ALOX12 gene and expressed primarily in platelets and skin. (wikipedia.org)
ALOX12 (arachidonate 12-lipoxygenase) gene is mapped to human chromosome 17p13.1. (sigmaaldrich.com)

Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. (creativebiomart.net)
The EGF-induced expression of 12-lipoxygenase mRNA was inhibited by transcription inhibitors such as actinomycin D and 5,6-dichlorobenzimidazole riboside. (elsevier.com)
Nonredox-type 5-lipoxygenase (5-LO) inhibitors such as ZM230487 or L-739.010 potently suppress leukotriene biosynthesis at low cellular peroxide tone. (nih.gov)
R) antagonists are preferred as anti-asthma and anti-rhinitis drugs because they are almost as effective as the 5-lipoxygenase inhibitors but have fewer side effects. (nii.ac.jp)
Since the 5-lipoxygenase pathway is abundantly expressed in atherosclerotic lesions, and 12/15-lipoxygenase is able to oxygenate polyunsaturated fatty acid esterified in the membranous phospholipids, 5-lipoxygenase or 12/15-lipoxygenase inhibitors may prevent progression of atherosclerosis. (nii.ac.jp)

The systematic name of this enzyme class is arachidonate:oxygen 8-oxidoreductase. (wikipedia.org)
Cyclooxygenase is well known asan enzyme responsible for the biosynthesis of prostaglandins and thromboxanes, and 5-lipoxygenase initiates the leukotriene synthesis. (nii.ac.jp)
There are two similar enzyme with still unknown biological functions, i.e., 12-lipoxygenase and 15 -lipoxygenase. (nii.ac.jp)
The enzyme activity in the supernatant was immunoprecipitated by the use of anti-human platelet 12-lipoxygenase antibody. (nii.ac.jp)
The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes. (biomol.com)
The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). (biomol.com)
This enzyme is part of a family of enzymes called arachidonate lipoxygenases. (nih.gov)
Unlike other lipoxygenases, the eLOX3 enzyme does not act directly on fatty acids. (nih.gov)
15-Lipoxygenase 2 (15-LOX2), the most abundant arachidonate (AA)-metabolizing enzyme expressed in adult human prostate, is a negative cell-cycle regulator in normal human prostate epithelial cells. (pubfacts.com)
This enzyme is assumed to be a 12-lipoxygenase (Hamberg et ai . (springer.com)
We previously reported that microgravity-dependent activation of 5-lipoxygenase (5-LOX) might play a central role in the initiation of apoptosis in human T lymphocytes, suggesting that the upregulation of this enzyme might be (at least in part) responsible for immunodepression observed in astronauts during space flights. (hindawi.com)

It inhibits synthesis of prostaglandins and leukotrienes by inhibition in the cyclooxygenase and lipoxygenase pathways of arachidonic acid metabolism. (springer.com)
Higgs GA, Mugridge KG, Moncada S, Vane JR (1984) Inhibition of tissue damage by the arachidonate lipoxygenase inhibitor BW755C. (springer.com)
Surprisingly, it was found that inhibition of 5-lipoxygenase does not inhibit Akt or ERKs which are known for their roles in cell survival. (clinicsinoncology.com)
Bromfield, Elizabeth G. / Pharmacological inhibition of arachidonate 15-lipoxygenase protects human spermatozoa against oxidative stress . (edu.au)
Lastly, the antioxidant activity was determined using four assays: DPPH and ABTS radicals scavenging assays, reducing power assay and 15-lipoxygenase inhibition assay. (gsajournals.org)
Characteristics and inhibition by a variety of phenyl hydrazones and comparisons with other lipoxygenases. (qmul.ac.uk)

The importance of the lipoxygenase-hepoxilin pathway in the mammalian epidermal barrier. (nih.gov)
The expression of epidermal lipoxygenases and transglutaminase-1 is perturbed by NIPAL4 mutations: indications of a common metabolic pathway essential for skin barrier homeostasis. (nih.gov)
One notable example of an important role of eicosanoids in cancer is the regulation of cancer cell survival by a group of arachidonic acid metabolites which are generated via the 5-lipoxygenase pathway. (clinicsinoncology.com)
Most notably, 5-lipoxygenase (LO) pathway products including LTC 4 and -D 4 , the slow-reacting substances of anaphylaxis, are considered causative agents in airway allergic responses, because they are potent bronchoconstrictors and inducers of leakage permeability changes ( 2 , 3 ). (jimmunol.org)
Less data about platelet lipoxygenase pathway, especially for its biological role, are available. (springer.com)
The 5‐lipoxygenase pathway is part of the innate immune system. (els.net)
Arachidonate 12-lipoxygenase is a non-heme iron-containing dioxygenase that functions in the leukotrienes pathway. (sigmaaldrich.com)

Jobard F, Lefèvre C, Karaduman A, Blanchet-Bardon C, Emre S, Weissenbach J, Ozgüc M, Lathrop M, Prud'homme JF, Fischer J. Lipoxygenase-3 (ALOXE3) and 12(R)-lipoxygenase (ALOX12B) are mutated in non-bullous congenital ichthyosiform erythroderma (NCIE) linked to chromosome 17p13.1. (nih.gov)

Mutation spectrum and functional analysis of epidermis-type lipoxygenases in patients with autosomal recessive congenital ichthyosis. (nih.gov)

Cloning and expression of arachidonate 12-lipoxygenase cDNA from porcine leukocytes. (elsevier.com)

The cloning of genes related to lipoxygenase-leukotriene metabolism prompted us to try to elucidate the role of leukotrienes in various inflammations. (nii.ac.jp)

When platelets are aggregated by some of these agents such as thrombin or collagen, polyunsaturated fatty acids (mainly arachidonic acid) are liberated from membrane phospholipids and then subsequently oxygenated via cyclooxygenase and/or lipoxygenase. (springer.com)
6 The combined cyclooxygenase/lipoxygenase (LO) inhibitor BW 755C completely blocked contractions in the females. (ahajournals.org)

It was first found as a product of arachidonic acid metabolism made by human and bovine platelets through their 12S-lipoxygenase (i.e. (wikipedia.org)

Biosynthesis of the Maresin Intermediate, 13S,14S-Epoxy-DHA, by Human 15-Lipoxygenase and 12-Lipoxygenase and Its Regulation through Negative Allosteric Modulators. (nih.gov)
Biosynthesis of lipoxygenase and cyclo-oxygenase products from [14C]-arachidonic acid by human colonic mucosa. (springer.com)
In addition, HSHE inhibited 5-lipoxygenase level and P. acnes-induced MMP-9 activity, and promoted collagen biosynthesis in vitro. (nih.gov)
5‐Lipoxygenase (5‐LOX) catalyses two steps in the biosynthesis of leukotrienes (LTs), lipid mediators of inflammation derived from arachidonic acid. (els.net)

[5] Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. (wikipedia.org)

We have shown previously that arachidonate 15-lipoxygenase type B (ALOX15B) is more highly expressed in human atherosclerotic lesions than in healthy arteries. (biomedsearch.com)

We have recently demonstrated that rat colon can convert 14 Carachidonic acid into both lipoxygenase and cyclo-oxygenase products, and that this metabolism is stimulated by the laxative phenolphthalein (Capasso et al 1984). (springer.com)
Lipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty acid hydroperoxidases. (genecards.org)
In conclusion, the leukotriene-lipoxygenase metabolism may be involved in the pathophysiology of acute inflammatory to chronic progressive disorders. (nii.ac.jp)
We think that more drugs modifying leukotriene-lipoxygenase metabolism will be introduced into clinical practice in the future. (nii.ac.jp)

Stimulation of colonic secretion by lipoxygenase metabolites of arachidonic acid. (springer.com)

The role of lipoxygenases in epidermis. (nih.gov)
Krieg P, Fürstenberger G. The role of lipoxygenases in epidermis. (nih.gov)

Both leukotrienes and the hydroxyeicosatetraenoids (HETEs) are products of 5-lipoxygenase activity. (clinicsinoncology.com)

2008) The role of diacylglyceride generation by phospholipase D and phosphatidic acid phosphatase in the activation of 5‐lipoxygenase in polymorphonuclear leukocytes. (els.net)
Scaffolding proteins that play an important role in the localization and activation of 5-LIPOXYGENASE. (harvard.edu)

In cultures of dorsal root ganglion neurons, we found that Sema3A treatment stimulates the synthesis of the eicosanoid, 12(S)-hydroxyeicosatetraenoic acid (HETE), whereas Sema3A-induced growth cone collapse is prevented when 12(S)-HETE synthesis is blocked with an inhibitor of 12/15-lipoxygenase (LO). (jneurosci.org)

Formation of the mammalian skin barrier requires both the essential fatty acid linoleate and the two lipoxygenases 12R-lipoxygenase (12R-LOX) and epidermal lipoxygenase-3 (eLOX3), although their roles are poorly understood. (biomedsearch.com)
Arachidonate has protumor-promoting action that is inhibited by linoleate in mouse skin carcinogenesis. (glycosciences.de)
The goal of this study was to determine whether the arachidonate content of epidermal phospholipids could be altered by increasing dietary levels of linoleate and whether specific metabolites of linoleate and arachidonate have dissimilar biological effects. (glycosciences.de)
In a series of tumor studies in which the quantity of dietary linoleate was incrementally increased, a slight reduction in phospholipid levels of arachidonate was observed that correlated with an increased phospholipid level of linoleate and a suppression in tumor yield. (glycosciences.de)
A comparison of the arachidonate lipoxygenase metabolite 12-hydroxyeicosatetraenoic acid (12-HETE) with the 13-hydroxyoctadecadienoic acid (13-HODE) lipoxygenase metabolite of linoleate revealed that 12-HETE has biological activities that mimic the phorbol ester tumor promoters, whereas 13-HODE has antithetical effects. (glycosciences.de)
Overall, these studies suggest that arachidonate and linoleate have opposing functions in the epidermis, particularly with regard to events involved in tumor development. (glycosciences.de)

This gene encodes a member of the lipoxygenase family of proteins. (nih.gov)
5-Lipoxygenase-Activating Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
This graph shows the total number of publications written about "5-Lipoxygenase-Activating Proteins" by people in Harvard Catalyst Profiles by year, and whether "5-Lipoxygenase-Activating Proteins" was a major or minor topic of these publication. (harvard.edu)
Below are the most recent publications written about "5-Lipoxygenase-Activating Proteins" by people in Profiles. (harvard.edu)

Arachidonic acid-15-lipoxygenase from rabbit peritoneal polymorphonuclear leukocytes. (genome.jp)
2003) Induction of 5‐lipoxygenase activation in polymorphonuclear leukocytes by 1‐oleoyl‐2‐acetylglycerol. (els.net)

This review covers the background to discovery of the two key lipoxygenases (LOX) involved in epidermal barrier function, 12R-LOX and eLOX3. (nih.gov)
Mutations associated with a congenital form of ichthyosis (NCIE) inactivate the epidermal lipoxygenases 12R-LOX and eLOX3. (medlineplus.gov)

Sub-primate mammals, such as the mouse, rat, rabbit, cow, and pig, express platelet type 12-lipoxygenase but also a leukocyte type 12-lipoxygenase (also termed 12/15-lipoxygenase, 12/15-LOX or 12/15-LO) which is an ortholog of, and metabolically equivalent to, human 15-LO-1 in that it forms predominantly 15(S)-HpETE with 12(S)-HpETE as a minor product. (wikipedia.org)

It has been reported that 12-hydroperoxyeicosatetraenoic acid (12-HPETE), the lipoxygenase product of AA, is able to inhibit both thromboxane synthase (Hammarström and Falardeau, 1977) and platelet diglyceride lipase (Rittenhouse-Simmons, 1980). (springer.com)

In addition, ATP is shown to inhibit, and phosphatidylcholine is shown to stimulate, 15-lipoxygenase, suggesting a regulatory role for these compounds in the lipoxygenation of arachidonic acid. (nih.gov)

3. Wallach, D.P. and Brown, V.R. A novel preparation of human platelet lipoxygenase. (qmul.ac.uk)

Publications] T.Ohtsuki: 'Reperfusion induces 5-lipoxygenase translocation and leukotriene C_4 Production in ischemic brain' Am.J.Physiol.268. (nii.ac.jp)

Arachidonate 12-Lipoxygenase (12-LOX) is one of several LOX isoforms that has iron as a cofactor and oxygenates polyunsaturated fatty acids. (atlasgeneticsoncology.org)
Lipoxygenases oxygenate polyunsaturated fatty acids. (els.net)

Subcellular localization and tumor-suppressive functions of 15-lipoxygenase 2 (15-LOX2) and its splice variants. (pubfacts.com)

Owing to the similarities in their genetic location, sequence and biological activities, leukocyte 12-LOX and 15-LOX-1 are often referred to as 12/15 lipoxygenase. (atlasgeneticsoncology.org)

abstract = "Comparison of relative arachidonate 15-lipoxygenase activities in different types of human cell reveals striking differences in levels of product generation. (wustl.edu)

Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega-hydroxyceramide for construction of the corneocyte lipid envelope. (nih.gov)
Arachidonate lipoxygenases add oxygen molecules at different locations on the arachidonic acid molecule, producing a variety of substances called fatty acid hydroperoxides. (medlineplus.gov)

According to Entrez Gene the arachidonate 12-lipoxygenase pseudogene (ALOX12P2) (HGNC: 13742) is located on 17p13.1. (atlasgeneticsoncology.org)

Anatomy2

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Chemicals and Drugs41

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes3

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Leukotriene A synthase activity of purified mouse skin arachidonate 8-lipoxygenase expressed in Escherichia coli. (1/145)

Identification of amino acid determinants of the positional specificity of mouse 8S-lipoxygenase and human 15S-lipoxygenase-2. (2/145)

8S-lipoxygenase products activate peroxisome proliferator-activated receptor alpha and induce differentiation in murine keratinocytes. (3/145)

A pertussis toxin-sensitive 8-lipoxygenase pathway is activated by a nicotinic acetylcholine receptor in aplysia neurons. (4/145)

Elevated expression of 12/15-lipoxygenase and cyclooxygenase-2 in a transgenic mouse model of prostate carcinoma. (5/145)

Upregulation of 8-lipoxygenase in the dermatitis of IkappaB-alpha-deficient mice. (6/145)

Arachidonate lipoxygenase (ALOX) and cyclooxygenase (COX) polymorphisms and colon cancer risk. (7/145)

Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality. (8/145)

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Arachidonate Lipoxygenases

Lipoxygenases

Lipoxygenase

Arachidonate 12-Lipoxygenase

Arachidonate 15-Lipoxygenase

Arachidonic Acid

Arachidonate 5-Lipoxygenase

Arachidonic Acids

Lipoxygenase Inhibitors

12-Hydroxy-5,8,10,14-eicosatetraenoic Acid

Linoleic Acids

Masoprocol

Lipid Peroxides

Leukotrienes

Hydroxyeicosatetraenoic Acids

Soybeans

Eicosanoids

Linoleic Acid

5,8,11,14-Eicosatetraynoic Acid

Prostaglandin-Endoperoxide Synthases

Fatty Acids, Unsaturated

Corylus

Chromatography, High Pressure Liquid

4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine

Lipoxins

Phospholipases A

Eicosanoic Acids

Blood Platelets

Phospholipases A2

Fatty Acids

Calcimycin

Cyclooxygenase Inhibitors

Solanum tuberosum

Kinetics

Linolenic Acids

Isoenzymes

Gas Chromatography-Mass Spectrometry

Substrate Specificity

Oxidation-Reduction

Reticulocytes

Prostaglandins E

Cyclooxygenase 1

Thromboxane B2

Phospholipids

Rabbits

Indomethacin

Prostaglandins

Oxygen

Leukotriene B4

Oxylipins

Fatty Acids, Essential

Phospholipases

Leukotriene A synthase activity of purified mouse skin arachidonate 8-lipoxygenase expressed in Escherichia coli. (1/145)

Identification of amino acid determinants of the positional specificity of mouse 8S-lipoxygenase and human 15S-lipoxygenase-2. (2/145)

8S-lipoxygenase products activate peroxisome proliferator-activated receptor alpha and induce differentiation in murine keratinocytes. (3/145)

A pertussis toxin-sensitive 8-lipoxygenase pathway is activated by a nicotinic acetylcholine receptor in aplysia neurons. (4/145)

Elevated expression of 12/15-lipoxygenase and cyclooxygenase-2 in a transgenic mouse model of prostate carcinoma. (5/145)

Upregulation of 8-lipoxygenase in the dermatitis of IkappaB-alpha-deficient mice. (6/145)

Arachidonate lipoxygenase (ALOX) and cyclooxygenase (COX) polymorphisms and colon cancer risk. (7/145)

Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality. (8/145)

LOX1.1 - Linoleate 9S-lipoxygenase A - Solanum lycopersicum (Tomato) - LOX1.1 gene & protein

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GFIT result for T01611

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Duke Computer Science

Arachidonate 12-lipoxygenase, 12R-type

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PQR | (5z,8z,10e,12s,14z)-12-hydroperoxy-5,8,10,14-icosatetraenoic acid