Arachidonate 15 lipoxygenase. Medical search

An enzyme of the oxidoreductase class primarily found in PLANTS. It catalyzes reactions between linoleate and other fatty acids and oxygen to form hydroperoxy-fatty acid derivatives.

An enzyme that catalyzes the oxidation of arachidonic acid to yield 5-hydroperoxyarachidonate (5-HPETE) which is rapidly converted by a peroxidase to 5-hydroxy-6,8,11,14-eicosatetraenoate (5-HETE). The 5-hydroperoxides are preferentially formed in leukocytes.

An enzyme that catalyzes the oxidation of arachidonic acid to yield 12-hydroperoxyarachidonate (12-HPETE) which is itself rapidly converted by a peroxidase to 12-hydroxy-5,8,10,14-eicosatetraenoate (12-HETE). The 12-hydroperoxides are preferentially formed in PLATELETS.

Compounds that bind to and inhibit that enzymatic activity of LIPOXYGENASES. Included under this category are inhibitors that are specific for lipoxygenase subtypes and act to reduce the production of LEUKOTRIENES.

An enzyme that catalyzes the oxidation of arachidonic acid to yield 15-hydroperoxyarachidonate (15-HPETE) which is rapidly converted to 15-hydroxy-5,8,11,13-eicosatetraenoate (15-HETE). The 15-hydroperoxides are preferentially formed in NEUTROPHILS and LYMPHOCYTES.

Enzymes catalyzing the oxidation of arachidonic acid to hydroperoxyarachidonates. These products are then rapidly converted by a peroxidase to hydroxyeicosatetraenoic acids. The positional specificity of the enzyme reaction varies from tissue to tissue. The final lipoxygenase pathway leads to the leukotrienes. EC 1.13.11.- .

An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.

A lipoxygenase metabolite of ARACHIDONIC ACID. It is a highly selective ligand used to label mu-opioid receptors in both membranes and tissue sections. The 12-S-HETE analog has been reported to augment tumor cell metastatic potential through activation of protein kinase C. (J Pharmacol Exp Ther 1995; 274(3):1545-51; J Natl Cancer Inst 1994; 86(15):1145-51)

A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.

A potent lipoxygenase inhibitor that interferes with arachidonic acid metabolism. The compound also inhibits formyltetrahydrofolate synthetase, carboxylesterase, and cyclooxygenase to a lesser extent. It also serves as an antioxidant in fats and oils.

Scaffolding proteins that play an important role in the localization and activation of 5-LIPOXYGENASE.

A dual inhibitor of both cyclooxygenase and lipoxygenase pathways. It exerts an anti-inflammatory effect by inhibiting the formation of prostaglandins and leukotrienes. The drug also enhances pulmonary hypoxic vasoconstriction and has a protective effect after myocardial ischemia.

The major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990)

Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes.

Eighteen-carbon essential fatty acids that contain two double bonds.

An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.

Phospholipases that hydrolyze one of the acyl groups of phosphoglycerides or glycerophosphatidates.

A 20-carbon unsaturated fatty acid containing 4 alkyne bonds. It inhibits the enzymatic conversion of arachidonic acid to prostaglandins E(2) and F(2a).

Phospholipases that hydrolyze the acyl group attached to the 2-position of PHOSPHOGLYCERIDES.

A class of compounds named after and generally derived from C20 fatty acids (EICOSANOIC ACIDS) that includes PROSTAGLANDINS; LEUKOTRIENES; THROMBOXANES, and HYDROXYEICOSATETRAENOIC ACIDS. They have hormone-like effects mediated by specialized receptors (RECEPTORS, EICOSANOID).

(2S-(2 alpha,3 beta(1E,3E,5Z,8Z)))-3-(1,3,5,8-Tetradecatetraenyl)oxiranebutanoic acid. An unstable allylic epoxide, formed from the immediate precursor 5-HPETE via the stereospecific removal of a proton at C-10 and dehydration. Its biological actions are determined primarily by its metabolites, i.e., LEUKOTRIENE B4 and cysteinyl-leukotrienes. Alternatively, leukotriene A4 is converted into LEUKOTRIENE C4 by glutathione-S-transferase or into 5,6-di-HETE by the epoxide-hydrolase. (From Dictionary of Prostaglandins and Related Compounds, 1990)

A non-steroidal anti-inflammatory agent (NSAID) that inhibits the enzyme cyclooxygenase necessary for the formation of prostaglandins and other autacoids. It also inhibits the motility of polymorphonuclear leukocytes.

Enzyme complexes that catalyze the formation of PROSTAGLANDINS from the appropriate unsaturated FATTY ACIDS, molecular OXYGEN, and a reduced acceptor.

20-carbon saturated monocarboxylic acids.

A doubly unsaturated fatty acid, occurring widely in plant glycosides. It is an essential fatty acid in mammalian nutrition and is used in the biosynthesis of prostaglandins and cell membranes. (From Stedman, 26th ed)

A group of LEUKOTRIENES; (LTC4; LTD4; and LTE4) that is the major mediator of BRONCHOCONSTRICTION; HYPERSENSITIVITY; and other allergic reactions. Earlier studies described a "slow-reacting substance of ANAPHYLAXIS" released from lung by cobra venom or after anaphylactic shock. The relationship between SRS-A leukotrienes was established by UV which showed the presence of the conjugated triene. (From Merck Index, 11th ed)

Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.

A stable, physiologically active compound formed in vivo from the prostaglandin endoperoxides. It is important in the platelet-release reaction (release of ADP and serotonin).

(11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.

Compounds or agents that combine with cyclooxygenase (PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES) and thereby prevent its substrate-enzyme combination with arachidonic acid and the formation of eicosanoids, prostaglandins, and thromboxanes.

A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.

Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.

Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.

Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.

The most common and most biologically active of the mammalian prostaglandins. It exhibits most biological activities characteristic of prostaglandins and has been used extensively as an oxytocic agent. The compound also displays a protective effect on the intestinal mucosa.

Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.

Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)

An acridine derivative formerly widely used as an antimalarial but superseded by chloroquine in recent years. It has also been used as an anthelmintic and in the treatment of giardiasis and malignant effusions. It is used in cell biological experiments as an inhibitor of phospholipase A2.

A class of enzymes that catalyze the hydrolysis of phosphoglycerides or glycerophosphatidates. EC 3.1.-.

FATTY ACIDS in which the carbon chain contains one or more double or triple carbon-carbon bonds.

Physiologically active compounds found in many organs of the body. They are formed in vivo from the prostaglandin endoperoxides and cause platelet aggregation, contraction of arteries, and other biological effects. Thromboxanes are important mediators of the actions of polyunsaturated fatty acids transformed by cyclooxygenase.

The rate dynamics in chemical or physical systems.

A phospholipid derivative formed by PLATELETS; BASOPHILS; NEUTROPHILS; MONOCYTES; and MACROPHAGES. It is a potent platelet aggregating agent and inducer of systemic anaphylactic symptoms, including HYPOTENSION; THROMBOCYTOPENIA; NEUTROPENIA; and BRONCHOCONSTRICTION.

Long chain organic acid molecules that must be obtained from the diet. Examples are LINOLEIC ACIDS and LINOLENIC ACIDS.

A prostaglandin that is a powerful vasodilator and inhibits platelet aggregation. It is biosynthesized enzymatically from PROSTAGLANDIN ENDOPEROXIDES in human vascular tissue. The sodium salt has been also used to treat primary pulmonary hypertension (HYPERTENSION, PULMONARY).

A group of FLAVONOIDS characterized with a 4-ketone.

Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to the hexahydroxy alcohol, myo-inositol. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid, myo-inositol, and 2 moles of fatty acids.

Trihydroxy derivatives of eicosanoic acids. They are primarily derived from arachidonic acid, however eicosapentaenoic acid derivatives also exist. Many of them are naturally occurring mediators of immune regulation.

A 20-carbon-chain fatty acid, unsaturated at positions 8, 11, and 14. It differs from arachidonic acid, 5,8,11,14-eicosatetraenoic acid, only at position 5.

Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.

Eighteen-carbon essential fatty acids that contain three double bonds.

A constitutively-expressed subtype of prostaglandin-endoperoxide synthase. It plays an important role in many cellular processes.

White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).

The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.

An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.

The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS.

The conjugation product of LEUKOTRIENE A4 and glutathione. It is the major arachidonic acid metabolite in macrophages and human mast cells as well as in antigen-sensitized lung tissue. It stimulates mucus secretion in the lung, and produces contractions of nonvascular and some VASCULAR SMOOTH MUSCLE. (From Dictionary of Prostaglandins and Related Compounds, 1990)

An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Azoles of two nitrogens at the 1,2 positions, next to each other, in contrast with IMIDAZOLES in which they are at the 1,3 positions.

A cytosolic phospholipase A2 group that plays an important role in the release of free ARACHIDONIC ACID, which in turn is metabolized to PROSTAGLANDINS by the CYCLOOXYGENASE pathway and to LEUKOTRIENES by the 5-LIPOXYGENASE pathway.

A nonapeptide messenger that is enzymatically produced from KALLIDIN in the blood where it is a potent but short-lived agent of arteriolar dilation and increased capillary permeability. Bradykinin is also released from MAST CELLS during asthma attacks, from gut walls as a gastrointestinal vasodilator, from damaged tissues as a pain signal, and may be a neurotransmitter.

A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.

A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.

Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.

Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.

A class of drugs designed to prevent leukotriene synthesis or activity by blocking binding at the receptor level.

Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.

Derivatives of PHOSPHATIDYLCHOLINES obtained by their partial hydrolysis which removes one of the fatty acid moieties.

Chemical agents that increase the permeability of biological or artificial lipid membranes to specific ions. Most ionophores are relatively small organic molecules that act as mobile carriers within membranes or coalesce to form ion permeable channels across membranes. Many are antibiotics, and many act as uncoupling agents by short-circuiting the proton gradient across mitochondrial membranes.

Fatty acid derivatives of glycerophosphates. They are composed of glycerol bound in ester linkage with 1 mole of phosphoric acid at the terminal 3-hydroxyl group and with 2 moles of fatty acids at the other two hydroxyl groups.

An enzyme found predominantly in platelet microsomes. It catalyzes the conversion of PGG(2) and PGH(2) (prostaglandin endoperoxides) to thromboxane A2. EC 5.3.99.5.

Enzymes that catalyze reversibly the formation of an epoxide or arene oxide from a glycol or aromatic diol, respectively.

The process of converting an acid into an alkyl or aryl derivative. Most frequently the process consists of the reaction of an acid with an alcohol in the presence of a trace of mineral acid as catalyst or the reaction of an acyl chloride with an alcohol. Esterification can also be accomplished by enzymatic processes.

The physiologically active and stable hydrolysis product of EPOPROSTENOL. Found in nearly all mammalian tissue.

A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.

Synthetic compounds that are analogs of the naturally occurring prostaglandin endoperoxides and that mimic their pharmacologic and physiologic activities. They are usually more stable than the naturally occurring compounds.

An inducibly-expressed subtype of prostaglandin-endoperoxide synthase. It plays an important role in many cellular processes and INFLAMMATION. It is the target of COX2 INHIBITORS.

The prototypical analgesic used in the treatment of mild to moderate pain. It has anti-inflammatory and antipyretic properties and acts as an inhibitor of cyclooxygenase which results in the inhibition of the biosynthesis of prostaglandins. Aspirin also inhibits platelet aggregation and is used in the prevention of arterial and venous thrombosis. (From Martindale, The Extra Pharmacopoeia, 30th ed, p5)

A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).

A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.

An unstable intermediate between the prostaglandin endoperoxides and thromboxane B2. The compound has a bicyclic oxaneoxetane structure. It is a potent inducer of platelet aggregation and causes vasoconstriction. It is the principal component of rabbit aorta contracting substance (RCS).

An antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase.

Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to an ethanolamine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and ethanolamine and 2 moles of fatty acids.

A group of fatty acids that contain 18 carbon atoms and a double bond at the omega 9 carbon.

Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.

Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.

Cell-surface receptors that bind LEUKOTRIENES with high affinity and trigger intracellular changes influencing the behavior of cells. The leukotriene receptor subtypes have been tentatively named according to their affinities for the endogenous leukotrienes LTB4; LTC4; LTD4; and LTE4.

A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)

A class of phenolic acids related to chlorogenic acid, p-coumaric acid, vanillic acid, etc., which are found in plant tissues. It is involved in plant growth regulation.

A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.

Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)

A group of 1,2-benzenediols that contain the general formula R-C6H5O2.

Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.

A group of physiologically active prostaglandin endoperoxides. They are precursors in the biosynthesis of prostaglandins and thromboxanes. Most frequently encountered member of this group is the prostaglandin G2.

Important polyunsaturated fatty acid found in fish oils. It serves as the precursor for the prostaglandin-3 and thromboxane-3 families. A diet rich in eicosapentaenoic acid lowers serum lipid concentration, reduces incidence of cardiovascular disorders, prevents platelet aggregation, and inhibits arachidonic acid conversion into the thromboxane-2 and prostaglandin-2 families.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Established cell cultures that have the potential to propagate indefinitely.

Enzymes of the isomerase class that catalyze the oxidation of one part of a molecule with a corresponding reduction of another part of the same molecule. They include enzymes converting aldoses to ketoses (ALDOSE-KETOSE ISOMERASES), enzymes shifting a carbon-carbon double bond (CARBON-CARBON DOUBLE BOND ISOMERASES), and enzymes transposing S-S bonds (SULFUR-SULFUR BOND ISOMERASES). (From Enzyme Nomenclature, 1992) EC 5.3.

A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.

A biologically active principle of SRS-A that is formed from LEUKOTRIENE D4 via a peptidase reaction that removes the glycine residue. The biological actions of LTE4 are similar to LTC4 and LTD4. (From Dictionary of Prostaglandins and Related Compounds, 1990)

Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

A non-steroidal anti-inflammatory agent with antipyretic and antigranulation activities. It also inhibits prostaglandin biosynthesis.

RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.

A group of 16-carbon fatty acids that contain no double bonds.

The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)

The addition of an organic acid radical into a molecule.

A nonsteroidal anti-inflammatory agent with analgesic properties used in the therapy of rheumatism and arthritis.

A sarcoma derived from deep fibrous tissue, characterized by bundles of immature proliferating fibroblasts with variable collagen formation, which tends to invade locally and metastasize by the bloodstream. (Stedman, 25th ed)

Compounds based on benzeneacetamide, that are similar in structure to ACETANILIDES.

Enzymes that catalyze the formation of acyl-CoA derivatives. EC 6.2.1.

A calcium-independent phospholipase A2 group that may play a role in membrane phospholipid remodeling and homeostasis by controling the levels of PHOSPHATIDYLCHOLINE in mammalian cell membranes.

An enzyme localized predominantly within the plasma membrane of lymphocytes. It catalyzes the transfer of long-chain fatty acids, preferentially unsaturated fatty acids, to lysophosphatides with the formation of 1,2-diacylglycero-3-phosphocholine and CoA. EC 2.3.1.23.

C22-unsaturated fatty acids found predominantly in FISH OILS.

GLYCEROPHOSPHOLIPIDS in which one of the two acyl chains is attached to glycerol with an ether alkenyl linkage instead of an ester as with the other glycerophospholipids.

The salts or esters of salicylic acids, or salicylate esters of an organic acid. Some of these have analgesic, antipyretic, and anti-inflammatory activities by inhibiting prostaglandin synthesis.

Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.

Cyclohexane ring substituted by one or more ketones in any position.

Organic compounds containing both the hydroxyl and carboxyl radicals.

An ethylmercury-sulfidobenzoate that has been used as a preservative in VACCINES; ANTIVENINS; and OINTMENTS. It was formerly used as a topical antiseptic. It degrades to ethylmercury and thiosalicylate.

The relationship between the dose of an administered drug and the response of the organism to the drug.

Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides.

Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

Anti-inflammatory agents that are non-steroidal in nature. In addition to anti-inflammatory actions, they have analgesic, antipyretic, and platelet-inhibitory actions.They act by blocking the synthesis of prostaglandins by inhibiting cyclooxygenase, which converts arachidonic acid to cyclic endoperoxides, precursors of prostaglandins. Inhibition of prostaglandin synthesis accounts for their analgesic, antipyretic, and platelet-inhibitory actions; other mechanisms may contribute to their anti-inflammatory effects.

A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.

Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.

Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.

Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.

A class of cell surface leukotriene receptors with a preference for leukotriene B4. Leukotriene B4 receptor activation influences chemotaxis, chemokinesis, adherence, enzyme release, oxidative bursts, and degranulation in polymorphonuclear leukocytes. There are at least two subtypes of these receptors. Some actions are mediated through the inositol phosphate and diacylglycerol second messenger systems.

An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.

An unsaturated fatty acid that is the most widely distributed and abundant fatty acid in nature. It is used commercially in the preparation of oleates and lotions, and as a pharmaceutical solvent. (Stedman, 26th ed)

A subcategory of secreted phospholipases A2 that includes enzymes isolated from a variety of sources. The creation of this group is based upon similarities in the structural determinants of the enzymes including a negatively charged carboxy-terminal segment.

Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.

Eighteen-carbon cyclopentyl polyunsaturated fatty acids derived from ALPHA-LINOLENIC ACID via an oxidative pathway analogous to the EICOSANOIDS in animals. Biosynthesis is inhibited by SALICYLATES. A key member, jasmonic acid of PLANTS, plays a similar role to ARACHIDONIC ACID in animals.

Elements of limited time intervals, contributing to particular results or situations.

A creeping annual plant species of the CUCURBITACEAE family. It has a rough succulent, trailing stem and hairy leaves with three to five pointed lobes.

A secreted phospholipase A2 subtype that contains a interfacial-binding region with specificity for PHOSPHATIDYLCHOLINE. This enzyme group may play a role in eliciting ARACHIDONIC ACID release from intact cellular membranes and from LOW DENSITY LIPOPROTEINS. Members of this group bind specifically to PHOSPHOLIPASE A2 RECEPTORS.

Dioxygenases that catalyze the peroxidation of methylene-interrupted UNSATURATED FATTY ACIDS.

An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.

A chemically diverse group of substances produced by various tissues in the body that cause slow contraction of smooth muscle; they have other intense but varied pharmacologic activities.

Compounds that inhibit the action of prostaglandins.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.

Enzymes from the transferase class that catalyze the transfer of acyl groups from donor to acceptor, forming either esters or amides. (From Enzyme Nomenclature 1992) EC 2.3.

A group of physiologically active prostaglandin endoperoxides. They are precursors in the biosynthesis of prostaglandins and thromboxanes. The most frequently encountered member of this group is the prostaglandin H2.

Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).

An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

A plant species of the genus SOLANUM, family SOLANACEAE. The starchy roots are used as food. SOLANINE is found in green parts.

Transport proteins that carry specific substances in the blood or across cell membranes.

(9 alpha,11 alpha,13E,15S)-9,11,15-Trihydroxyprost-13-en-1-oic acid (PGF(1 alpha)); (5Z,9 alpha,11,alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic acid (PGF(2 alpha)); (5Z,9 alpha,11 alpha,13E,15S,17Z)-9,11,15-trihydroxyprosta-5,13,17-trien-1-oic acid (PGF(3 alpha)). A family of prostaglandins that includes three of the six naturally occurring prostaglandins. All naturally occurring PGF have an alpha configuration at the 9-carbon position. They stimulate uterine and bronchial smooth muscle and are often used as oxytocics.

Benzene rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups.

Precursors in the biosynthesis of prostaglandins and thromboxanes from arachidonic acid. They are physiologically active compounds, having effect on vascular and airway smooth muscles, platelet aggregation, etc.

Proteins prepared by recombinant DNA technology.

A class of lipoproteins of small size (18-25 nm) and light (1.019-1.063 g/ml) particles with a core composed mainly of CHOLESTEROL ESTERS and smaller amounts of TRIGLYCERIDES. The surface monolayer consists mostly of PHOSPHOLIPIDS, a single copy of APOLIPOPROTEIN B-100, and free cholesterol molecules. The main LDL function is to transport cholesterol and cholesterol esters to extrahepatic tissues.

A stable prostaglandin endoperoxide analog which serves as a thromboxane mimetic. Its actions include mimicking the hydro-osmotic effect of VASOPRESSIN and activation of TYPE C PHOSPHOLIPASES. (From J Pharmacol Exp Ther 1983;224(1): 108-117; Biochem J 1984;222(1):103-110)

Mononuclear phagocytes derived from bone marrow precursors but resident in the peritoneum.

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.

Phospholipids which have an alcohol moiety in ethereal linkage with a saturated or unsaturated aliphatic alcohol. They are usually derivatives of phosphoglycerols or phosphatidates. The other two alcohol groups of the glycerol backbone are usually in ester linkage. These compounds are widely distributed in animal tissues.

The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.

Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.

A dideoxynucleoside compound in which the 3'-hydroxy group on the sugar moiety has been replaced by a hydrogen. This modification prevents the formation of phosphodiester linkages which are needed for the completion of nucleic acid chains. The compound is an inhibitor of HIV replication, acting as a chain-terminator of viral DNA by binding to reverse transcriptase. Its principal side effect is nephrotoxicity. In vivo, dideoxyadenosine is rapidly metabolized to DIDANOSINE (ddI) by enzymatic deamination; ddI is then converted to dideoxyinosine monophosphate and ultimately to dideoxyadenosine triphosphate, the putative active metabolite.

A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.

Compounds that bind to and inhibit the action of 5-LIPOXYGENASE-ACTIVATING PROTEINS.

Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.

A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.

One of the biologically active principles of SRS-A. It is generated from LEUKOTRIENE C4 after partial hydrolysis of the peptide chain, i.e., cleavage of the gamma-glutamyl portion. Its biological actions include stimulation of vascular and nonvascular smooth muscle, and increases in vascular permeability. (From Dictionary of Prostaglandins and Related Compounds, 1990)

A phenothiazine with actions similar to CHLORPROMAZINE. It is used as an antipsychotic and an antiemetic.

Cell surface proteins that bind eicosanoids with high affinity and trigger intracellular changes influencing the behavior of cells. Among the eicosanoid receptors are receptors for the prostaglandins, thromboxanes, and leukotrienes.

A group of alicyclic hydrocarbons with the general formula R-C5H9.

Derivatives of phosphatidic acid in which the hydrophobic regions are composed of two fatty acids and a polar alcohol is joined to the C-3 position of glycerol through a phosphodiester bond. They are named according to their polar head groups, such as phosphatidylcholine and phosphatidylethanolamine.

A flavoprotein enzyme that catalyzes the univalent reduction of OXYGEN using NADPH as an electron donor to create SUPEROXIDE ANION. The enzyme is dependent on a variety of CYTOCHROMES. Defects in the production of superoxide ions by enzymes such as NADPH oxidase result in GRANULOMATOUS DISEASE, CHRONIC.

Proteins, usually acting in oxidation-reduction reactions, containing iron but no porphyrin groups. (Lehninger, Principles of Biochemistry, 1993, pG-10)

A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.

A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.

The space enclosed by the peritoneum. It is divided into two portions, the greater sac and the lesser sac or omental bursa, which lies behind the STOMACH. The two sacs are connected by the foramen of Winslow, or epiploic foramen.

The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

GLYCEROL esterified with FATTY ACIDS.

Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.

7-Hydroxycoumarins. Substances present in many plants, especially umbelliferae. Umbelliferones are used in sunscreen preparations and may be mutagenic. Their derivatives are used in liver therapy, as reagents, plant growth factors, sunscreens, insecticides, parasiticides, choleretics, spasmolytics, etc.

Fatty acid esters of cholesterol which constitute about two-thirds of the cholesterol in the plasma. The accumulation of cholesterol esters in the arterial intima is a characteristic feature of atherosclerosis.

The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

A common saturated fatty acid found in fats and waxes including olive oil, palm oil, and body lipids.

An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.

Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.

Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.

The process of cleaving a chemical compound by the addition of a molecule of water.

An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.

Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)

cDNA cloning of 15-lipoxygenase type 2 and 12-lipoxygenases of bovine corneal epithelium. (1/405)

Bovine corneal epithelium contains arachidonate 12- and 15-lipoxygenase activity, while human corneal epithelium contains only 15-lipoxygenase activity. Our purpose was to identify the corneal 12- and 15-lipoxygenase isozymes. We used cDNA cloning to isolate the amino acid coding nucleotide sequences of two bovine lipoxygenases. The translated sequence of one lipoxygenase was 82% identical with human 15-lipoxygenase type 2 and 75% identical with mouse 8-lipoxygenase, whereas the other translated nucleotide sequence was 87% identical with human 12-lipoxygenase of the platelet type. Expression of 15-lipoxygenase type 2 and platelet type 12-lipoxygenase mRNAs were detected by Northern analysis. In addition to these two lipoxygenases, 12-lipoxygenase of leukocyte (tracheal) type was detected by polymerase chain reaction (PCR), sequencing, and Northern analysis. Finally, PCR and sequencing suggested that human corneal epithelium contains 15-lipoxygenase types 1 and 2. (+info)

Oxidation of low density lipoprotein and plasma by 15-lipoxygenase and free radicals. (2/405)

It is generally accepted that the oxidation of pentadiene structures of polyunsaturated lipids by lipoxygenase (LOX) is regio- and enantio-specific, while the free radical-mediated lipid peroxidation gives stereo-random racemic products. It was confirmed that the oxidation of human low density lipoprotein (LDL) by 15-LOX from rabbit reticulocytes gave phosphatidylcholine (PC) and cholesteryl ester (CE) hydroperoxides regio-, stereo- and enantio-specifically. 15-LOX also oxidized human plasma to give specific PC and CE hydroperoxides in spite of the presence of high concentrations of antioxidants. More CE hydroperoxides were formed than PC hydroperoxides from LDL, but the reverse order was observed for plasma oxidation. The S/R ratio of the hydroperoxides decreased during long time incubation but remained significantly larger than one, while free radical-mediated oxidation of LDL and plasma gave racemic products. (+info)

Tissue-specific translational regulation of alternative rabbit 15-lipoxygenase mRNAs differing in their 3'-untranslated regions. (3/405)

By screening a rabbit reticulocyte library, an alternative 15-LOX transcript of 3.6 kb (15-LOX mRNA2) was detected containing a 1019 nt longer 3'-untranslated region (UTR2) than the main 2.6 kb mRNA (15-LOX mRNA1). In anaemic animals, northern blotting showed that 15-LOX mRNA2 was predominantly expressed in non-erythroid tissues, whereas 15-LOX mRNA1 was exclusively expressed in red blood cells and bone marrow. The 15-LOX 3'-UTR2 mRNA2 contained a novel 8-fold repetitive CU-rich motif, 23 nt in length (DICE2). This motif is related but not identical to the 10-fold repetitive differentiation control element (DICE1) of 19 nt residing in the 15-LOX UTR1 mRNA1. DICE1 was shown to interact with human hnRNP proteins E1 and K, thereby inhibiting translation. From tissues expressing the long 15-LOX mRNA2, two to three unidentified polypeptides with molecular weights of 53-55 and 90-93 kDa which bound to DICE2 were isolated by RNA affinity chromatography. A 93 kDa protein from lung cytosol, which was selected by DICE2 binding, was able to suppress translational inhibition of 15-LOX mRNA2, but not of 15-LOX mRNA1, by hnRNP E1. A possible interaction between DICE1/DICE2 cis / trans factors in translational control of 15-LOX synthesis is discussed. Furthermore, the 3'-terminal part of the highly related rabbit leukocyte-type 12-LOX gene was analysed. Very similar repetitive CU-rich elements of the type DICE1 (20 repeats) and DICE2 (nine repeats) were found in the part corresponding to the 3'-UTR of the mRNA. (+info)

Regulation of 15-lipoxygenase expression and mucus secretion by IL-4 in human bronchial epithelial cells. (4/405)

Our laboratory has recently shown that mucus differentiation of cultured normal human tracheobronchial epithelial (NHTBE) cells is accompanied by the increased expression of 15-lipoxygenase (15-LO). We used differentiated NHTBE cells to investigate the regulation of 15-LO expression and mucus secretion by inflammatory cytokines. Interleukin (IL)-4 and IL-13 dramatically enhanced the expression of 15-LO, whereas tumor necrosis factor-alpha, IL-1beta, and interferon (IFN)-gamma had no effect. These cytokines did not increase the expression of cyclooxygenase-2, with the exception of a modest induction by IL-1beta. The IL-4-induced 15-LO expression was concentration dependent, and mRNA and protein expression increased within 3 and 6 h, respectively, after IL-4 treatment. In metabolism studies with intact cells, 15-hydroxyeicosatetraenoic acid (15-HETE) and 13-hydroxyoctadecadienoic acid (13-HODE) were the major metabolites formed from exogenous arachidonic acid and linoleic acid. No prostaglandins were detected. IL-4 treatment dramatically increased the formation of 13-HODE and 15-HETE compared with that in untreated NHTBE cells, and several additional 15-LO metabolites were observed. Pretreatment of NHTBE cells with IFN-gamma or dexamethasone did not inhibit the IL-4-induced expression of 15-LO except at high concentrations (100 ng/ml of IFN-gamma and 10 microM dexamethasone). IL-4 treatment inhibited mucus secretion and attenuated the expression of the mucin genes MUC5AC and MUC5B at 12-24 h after treatment. Addition of 15-HETE precursor and 13-HODE precursor to the cultures did not alter mucin secretion or mucin gene expression. On the basis of the data presented, we conclude that the increase in 15-LO expression by IL-4 and attenuation of mucus secretion may be independent biological events. (+info)

Effects of mutant p53 expression on human 15-lipoxygenase-promoter activity and murine 12/15-lipoxygenase gene expression: evidence that 15-lipoxygenase is a mutator gene. (5/405)

Human 15-lipoxygenase (h15-LO) is present on chromosome 17p13.3 in close proximity to the tumor-suppressor gene, p53. 15-LO is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. The murine BALB/c embryo fibroblast cell line, (10)1val, expresses p53 in mutant (mt) conformation when grown at 39 degrees C and in wild-type conformation when grown at 32 degrees C. Transfection of h15-LO promoter constructs (driving luciferase reporter) into (10)1val cells and into p53-deficient (10)1 cells resulted in a marked increase in h15-LO promoter activity in (10)1val cells at 39 degrees C, but not at 32 degrees C, or as compared with (10)1 cells. Transfection of h15-LO promoter deletion constructs, however, resulted in total loss of activity in both cell types at 32 degrees C and 39 degrees C. Cotransfection of (10)1 cells with h15-LO promoter (driving luciferase reporter) along with increasing levels of a mt p53 expression vector demonstrated dose-dependent capacity of mt p53 to induce 15-LO promoter activity. No effect was observed with wild-type p53. In contrast to h15-LO promoter activity, (10)1val cells had significantly lower levels of endogenous (murine) 12/15-LO (mouse analog of h15-LO) mRNA and protein when grown at 39 degrees C compared with cells grown at 32 degrees C. Our data support the hypothesis that loss of a tumor-suppressor gene (p53), or "gain-of-function activities" resulting from the expression of its mutant forms, regulates 15-LO promoter activity in man and in mouse, albeit in directionally opposite manners. The studies define a direct link between 15-LO activity and an established tumor-suppressor gene located in close chromosomal proximity. (+info)

Disruption of the 12/15-lipoxygenase gene diminishes atherosclerosis in apo E-deficient mice. (6/405)

Atherosclerosis may be viewed as an inflammatory disease process that includes early oxidative modification of LDLs, leading to foam cell formation. This "oxidation hypothesis" has gained general acceptance in recent years, and evidence for the role of lipoxygenases in initiation of, or participation in, the oxidative process is accumulating. However, the relative contribution of macrophage-expressed lipoxygenases to atherogenesis in vivo remains unknown. Here, we provide in vivo evidence for the role of 12/15-lipoxygenase in atherogenesis and demonstrate diminished plasma IgG autoantibodies to oxidized LDL epitopes in 12/15-lipoxygenase knockout mice crossbred with atherosclerosis-prone apo E-deficient mice (apo E-/-/L-12LO-/-). In chow-fed 15-week-old apo E-/-/L-12LO-/- mice, the extent of lesions in whole-aorta en face preparations (198 +/- 60 microm2) was strongly reduced (P < 0.001, n = 12) when compared with 12/15-lipoxygenase-expressing controls (apo E-/-/L-12LO+/+), which showed areas of lipid deposition (15,700 +/- 2,688 microm2) in the lesser curvature of the aortic arch, branch points, and in the abdominal aorta. These results were observed despite cholesterol, triglyceride, and lipoprotein levels that were similar to those in apo E-deficient mice. Evidence for reduced lesion development was observed even at 1 year of age in apo E-/-/L-12LO-/- mice. The combined data indicate a role for 12/15-lipoxygenase in the pathogenesis of atherosclerosis and suggest that inhibition of this enzyme may decrease disease progression. (+info)

All ApoB-containing lipoproteins induce monocyte chemotaxis and adhesion when minimally modified. Modulation of lipoprotein bioactivity by platelet-activating factor acetylhydrolase. (7/405)

Mildly oxidized LDL has many proinflammatory properties, including the stimulation of monocyte chemotaxis and adhesion, that are important in the development of atherosclerosis. Although ApoB-containing lipoproteins other than LDL may enter the artery wall and undergo oxidation, very little is known regarding their proinflammatory potential. LDL, IDL, VLDL, postprandial remnant particles, and chylomicrons were mildly oxidized by fibroblasts overexpressing 15-lipoxygenase (15-LO) and tested for their ability to stimulate monocyte chemotaxis and adhesion to endothelial cells. When conditioned on 15-LO cells, LDL, IDL, but not VLDL increased monocyte chemotaxis and adhesion approximately 4-fold. Chylomicrons and postprandial remnant particles were also bioactive. Although chylomicrons had a high 18:1/18:2 ratio, similar to that of VLDL, and should presumably be less susceptible to oxidation, they contained (in contrast to VLDL) essentially no platelet-activating factor acetylhydrolase (PAF-AH) activity. Because PAF-AH activity of lipoproteins may be reduced in vivo by oxidation or glycation, LDL, IDL, and VLDL were treated in vitro to reduce PAF-AH activity and then conditioned on 15-lipoxygenase cells. All 3 PAF-AH-depleted lipoproteins, including VLDL, exhibited increased stimulation of monocyte chemotaxis and adhesion. In a similar manner, lipoproteins from Japanese subjects with a deficiency of plasma PAF-AH activity were also markedly more bioactive, and stimulated monocyte adhesion nearly 2-fold compared with lipoproteins from Japanese control subjects with normal plasma PAF-AH. For each lipoprotein, bioactivity resided in the lipid fraction and monocyte adhesion could be blocked by PAF-receptor antagonists. These data suggest that the susceptibility of plasma lipoproteins to develop proinflammatory activity is in part related to their 18:1/18:2 ratio and PAF-AH activity, and that bioactive phospholipids similar to PAF are generated during oxidation of each lipoprotein. Moreover, LDL, IDL, postprandial remnant particles, and chylomicrons and PAF-AH-depleted VLDL all give rise to proinflammatory lipids when mildly oxidized. (+info)

The inhibition of mammalian 15-lipoxygenases by the anti-inflammatory drug ebselen: dual-type mechanism involving covalent linkage and alteration of the iron ligand sphere. (8/405)

Mammalian lipoxygenases have been implicated in inflammation and atherosclerosis and, thus, lipoxygenase inhibitors may be of pharmacological interest. In cells, lipoxygenases occur in a catalytically silent ground state that requires activation to become active. We found that the seleno-organic drug ebselen [2-phenyl-1, 2-benzisoselenazol-3(2H)-one], which exhibits anti-inflammatory properties, irreversibly inhibited pure rabbit 15-lipoxygenase, with an IC50 in the nM range when preincubated with the enzyme in the absence of fatty acid substrates. Subsequent dialysis, gel filtration, or substrate addition did not restore the enzyme activity, and experiments with [14C]ebselen indicated a covalent linkage of the drug. The presence of sulfhydryl compounds in the incubation mixture prevented both enzyme labeling and inactivation, but we did not see any reactivation when sulfhydryl compounds were added afterward. X-ray absorption studies indicated that ebselen did alter the geometry of the iron ligand sphere, and the data are consistent with an iron complexation by the drug. When fatty acid substrate was present during lipoxygenase-ebselen interaction, the inhibitory potency was strongly reduced and a competitive mode of action was observed. These data suggest that ebselen inactivated the catalytically silent ground-state lipoxygenase irreversibly by covalent linkage and alteration of the iron ligand sphere. In contrast, it functions as a competitive inhibitor of the catalytically active enzyme species. The pharmacological relevance of ebselen as a potential in vivo lipoxygenase inhibitor will be discussed. (+info)

5-lipoxygenase, 12-lipoxygenase, and 15-lipoxygenase-2, and selected metabolites of the latter lipoxygenases show no such ... Kuhn, Hartmut; Walther, Matthias; Kuban, Ralf Jürgen (2002). "Mammalian arachidonate 15-lipoxygenases". Prostaglandins & Other ... "Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid ... Evidence for a role of the reticulocyte lipoxygenase in the maturation of red cells". The Journal of Biological Chemistry. 265 ...

https://en.wikipedia.org/wiki/13-oxoODE

5-lipoxygenase, 12-lipoxygenase, and 15-lipoxygenase-2, and selected metabolites of the latter lipoxygenases show no such ... Kuhn, Hartmut; Walther, Matthias; Kuban, Ralf Jürgen (2002). "Mammalian arachidonate 15-lipoxygenases". Prostaglandins & Other ... "Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid ... Folcik, V A; Nivar-Aristy, R A; Krajewski, L P; Cathcart, M K (1995). "Lipoxygenase contributes to the oxidation of lipids in ...

https://en.wikipedia.org/wiki/13-Hydroxyoctadecadienoic_acid

Arachidonate 15-lipoxygenase type II is an enzyme that in humans is encoded by the ALOX15B gene. ALOX15B, also known as 15- ... Xu XC, Shappell SB, Liang Z, Song S, Menter D, Subbarayan V, Iyengar S, Tang DG, Lippman SM (2003). "Reduced 15S-lipoxygenase-2 ... "Entrez Gene: ALOX15B arachidonate 15-lipoxygenase, type B". Human ALOX15B genome location and ALOX15B gene details page in the ... Brash AR, Boeglin WE, Chang MS (Jun 1997). "Discovery of a second 15S-lipoxygenase in humans". Proceedings of the National ...

https://en.wikipedia.org/wiki/ALOX15B

... an arachidonate lipoxygenase product". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1136 (3): 247-52. doi: ... inhibitor of 12-lipoxygenase but not other human lipoxygenases. This effect could also have anti-inflammatory and anti- ... Oxygenated by 5-lipoxygenase (ALOX5 to form its 5,6-trans epoxide derivative which may then rearrange to the lipoxins (LX), ... Brash, A. R.; Boeglin, W. E.; Chang, M. S. (1997). "Discovery of a second 15S-lipoxygenase in humans". Proceedings of the ...

https://en.wikipedia.org/wiki/15-Hydroxyeicosatetraenoic_acid

ALOX12B (i.e. arachidonate 12-lipoxygenase, 12R type) forms R chirality products, i.e. 12R-HpETE and 12R-HETE. Similarly, ... They include (HEPE is hydroxy-eicsapentaenoic acid): 5-HEPE (see Arachidonate 5-lipoxygenase#Eicosapentaenoic acid), 12-HEPE, ... Lipoxygenases (LOXs): 5-Lipoxygenase (5-LOX or ALOX5) initiates the metabolism of arachidonic acid to 5- ... see Arachidonate 5-lipoxygenase#Eicosapentaenoic acid); PGE3, PGD3, PGF3α, and Δ(17)-6-keto PGF1α; PGI3 (see Essential fatty ...

https://en.wikipedia.org/wiki/Eicosanoid

... leukocyte-type arachidonate 12-lipoxygenase, or arachidonate omega-6 lipoxygenase. The second discovered human 15-lipoxygenase ... omega-6 lipoxygenase) from human leukocytes. Purification and structural homology to other mammalian lipoxygenases". The ... whereas the most unrelated lipoxygenase (5-lipoxygenase) was mapped to chromosome 10. Kelavkar and Badr (1999) stated that the ... ALOX15 (also termed arachidonate 15-lipoxygenase, 15-lipoxygenase-1, 15-LO-1, 15-LOX-1) is, like other lipoxygenases, a seminal ...

https://en.wikipedia.org/wiki/ALOX15

... oxygenation of this arachidonic acid by activated arachidonate 5-lipoxygenase (ALOX5) to form 5(S)-hydroperoxy-6E,8Z,11Z,14Z- ... Second, human platelets use 12-lipoxygenase to metabolize 5-oxo-ETE to 5-oxo-12(S)-hydroperxy-eicosatetraenoat which is rapidly ... This transcellular production typically involves the limited variety of cell types that express active 5-lipoxygenase, lack ... First, human eosinophils use Arachidonate 15-lipoxygenase-1 (or possibly Arachidonate 15-lipoxygenase-2 to metabolize 5-oxo-ETE ...

https://en.wikipedia.org/wiki/5-Oxo-eicosatetraenoic_acid

In humans, Arachidonate 12-lipoxygenase (12-LO, 12-LOX, ALO12, or platelet type 12-lipoxygenase) is encoded by the ALOX12 gene ... 12-lipoxygenase gene, see lipoxygenase#mouse lipoxygenases) are resistant to a) streptozotocin-induced, b) high fat diet- ... Arachidonate 12-lipoxygenase, 12R type, also termed 12RLOX and encoded by the ALOX12B gene, is expressed primarily in skin and ... Nugteren, D. H. (1975). "Arachidonate lipoxygenase in blood platelets". Biochimica et Biophysica Acta. 380 (2): 299-307. doi: ...

https://en.wikipedia.org/wiki/12-Hydroxyeicosatetraenoic_acid

... , also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13 ... Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. It transforms essential fatty acids (EFA) ... "Alox5 - arachidonate 5-lipoxygenase". WikiGenes. Fahel JS, de Souza MB, Gomes MT, Corsetti PP, Carvalho NB, Marinho FA, de ... Arachidonate+5-Lipoxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Human ALOX5 genome location ...

https://en.wikipedia.org/wiki/Arachidonate_5-lipoxygenase

... arachidonate 5-lipoxygenase MeSH D12.776.556.579.374.450.025.025 - arachidonate 12-lipoxygenase MeSH D12.776.556.579.374.450. ... lipoxygenase MeSH D12.776.556.579.374.450.025 - arachidonate lipoxygenases MeSH D12.776.556.579.374.450.025.020 - ... arachidonate 15-lipoxygenase MeSH D12.776.556.579.374.687 - retinal dehydrogenase MeSH D12.776.556.579.374.925 - tyrosine 3- ... interleukin-15 MeSH D12.776.467.374.400.505.516 - interleukin-16 MeSH D12.776.467.374.400.505.517 - interleukin-17 MeSH D12.776 ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D12.776)

... arachidonate 5-lipoxygenase MeSH D08.811.682.690.416.444.050.060 - arachidonate 12-lipoxygenase MeSH D08.811.682.690.416.444. ... lipoxygenase MeSH D08.811.682.690.416.444.050 - arachidonate lipoxygenases MeSH D08.811.682.690.416.444.050.055 - ... 050.065 - arachidonate 15-lipoxygenase MeSH D08.811.682.690.416.444.525 - protocatechuate 3,4-dioxygenase MeSH D08.811.682.690. ... beta-carotene 15,15'-monooxygenase MeSH D08.811.682.690.708.392.468 - Linoleoyl-CoA desaturase MeSH D08.811.682.690.708.392.625 ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D08)

... s are compounds that slow or stop the action of the arachidonate 5-lipoxygenase (5- ... Antileukotriene agents Arachidonate 5-lipoxygenase ALOX5-inhibiting drugs David L. Nelson, Michael M. Cox. Lehninger's ... 39: 1302-4. PMID 12018529.CS1 maint: multiple names: authors list (link) 1. Arachidonate 5-lipoxygenase ...Specific function: ... Zileuton is a specific inhibitor of 5-lipoxygenase and thus inhibits leukotriene (LTB4, LTC4, LTD4, and LTE4) formation. Both ...

https://en.wikipedia.org/wiki/Arachidonate_5-lipoxygenase_inhibitor

... also termed arachidonate-5-lipoxygenase, 5-lipoxygenase, 5-LO, and 5-LOX). ALOX5 metabolizes arachidonic acid to its ... 12-Lipoxygenase (i.e. ALOX12) to metabolize 5(S)-HETE to 5(S),12(S)-diHETE. The activity of this product has not yet been fully ... by the arachidonate 15-Lipoxygenase-1-based or arachidonate 15-lipoxygenased-2-based metabolism of 5-oxo-ETE; and f) conversion ... The relative potencies of 5-oxo-ETE, 5-oxo-15(S)-HETE, 5-(S)-HETE, 5-(S),15-(S)-diHETE, 5-oxo-20-hydroxy-ETE, 5-(S),20-diHETE, ...

https://en.wikipedia.org/wiki/5-Hydroxyeicosatetraenoic_acid

5-Lipoxygenase activating protein/Arachidonate 5-lipoxygenase. *LTA4 hydrolase (B4 synthesis). *LTC4 synthase ... arachidonate 15-lipoxygenase activity. • metal ion binding. • heme binding. • enzyme binding. • oxidoreductase activity. • ... lipoxygenase pathway. • cellular response to mechanical stimulus. • response to manganese ion. • angiogenesis. • cellular ... 10-difluoroarachidonic acid with PGH synthase and soybean lipoxygenase". Journal of the American Chemical Society. 109 (12): ...

https://en.wikipedia.org/wiki/Cyclooxygenase-2

Bryant, R.W., Bailey, J.M., Schewqe, T. and Rapoport, S.M. (1982). „Positional specificity of a reticulocyte lipoxygenase. ... B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, ... Oliw, E.H. & Sprecher, H. (1989). „Metabolism of polyunsaturated fatty acids by an (n-6)-lipoxygenase associated with human ... Primary structure of soybean lipoxygenase-1". J. Biol. Chem. 262: 10080-10085. PMID 3112136.. CS1 одржавање: Вишеструка имена: ...

https://sr.wikipedia.org/wiki/Arahidonat_15-lipoksigenaza

lipoxygenase: Arachidonate 5-lipoxygenase. *Arachidonate 12-lipoxygenase/ALOX12. *Arachidonate 8-lipoxygenase. *Arachidonate 15 ...

https://en.wikipedia.org/wiki/1,2-dihydroxynaphthalene_dioxygenase

Arachidonate 5-lipoxygenase-activating protein also known as 5-lipoxygenase activating protein, or FLAP, is a protein that in ... Zintzaras E, Rodopoulou P, Sakellaridis N (March 2009). "Variants of the arachidonate 5-lipoxygenase-activating protein ( ... "Associations of genetic polymorphisms of arachidonate 5-lipoxygenase-activating protein with risk of coronary artery disease in ... "Cytogenetic and radiation hybrid mapping of human arachidonate 5-lipoxygenase-activating protein (ALOX5AP) to chromosome 13q12 ...

https://en.wikipedia.org/wiki/5-lipoxygenase-activating_protein

"ALOX5 arachidonate 5-lipoxygenase [Homo sapiens (human)] - Gene - NCBI". Haeggström, J. Z.; Funk, C. D. (2011). "Lipoxygenase ... Arachidonate 12-lipoxygenase (ALOX12) (EC 1.13.11.31InterPro: IPR001885), also termed 12-lipoxygenase, platelet type platelet ... LOX-DB - LipOXygenases DataBase Lipoxygenases iron-binding region in PROSITE PDB: 1YGE - structure of lipoxygenase-1 from ... There are several lipoxygenase structures known including: soybean lipoxygenase L1 and L3, coral 8-lipoxygenase, human 5- ...

https://en.wikipedia.org/wiki/Lipoxygenase

Arachidonate 5-lipoxygenase Arachidonate 15-lipoxygenase Leukotriene 15-Hydroxyicosatetraenoic acid Greene ER, Huang S, Serhan ... The primary product of the lipoxygenase, 15-HPETE is believed to react with the enzyme further to produce the 14,15-epoxide, ... lipoxygenases (5-LOX, 12-LOX, 15-LOXa, 15-LOXb), and cytochrome P450s, respectively. These pathways are the target of approved ... is then converted to a series of eoxins by the same enzymes that metabolize the 5-lipoxygenase product of arachidonic acid ...

https://en.wikipedia.org/wiki/Eoxin

... also known as arachidonate 12-lipoxygenase, 12-lipoxygenase, 12S-Lipoxygenase, 12-LOX, and 12S-LOX is a lipoxygenase-type ... "Entrez Gene: ALOX12 arachidonate 12-lipoxygenase". Yamamoto S, Suzuki H, Ueda N (March 1997). "Arachidonate 12-lipoxygenases". ... arachidonate:oxygen 12-oxidoreductase, Delta12-lipoxygenase, 12Delta-lipoxygenase, and C-12 lipoxygenase. ALOX12, often termed ... Nugteren DH (February 1975). "Arachidonate lipoxygenase in blood platelets". Biochimica et Biophysica Acta (BBA) - Lipids and ...

https://en.wikipedia.org/wiki/ALOX12

Arachidonate 5-lipoxygenase has been identified as playing a significant role in the survival of prostate cancer cells.[186][ ... and the American College of Physicians discourages screening for those who are expected to live less than ten to fifteen years ... arachidonate 5-lipoxygenase inhibitors produce massive, rapid programmed cell death in prostate cancer cells.[186][187][188] ... "Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells". Proc. Natl. Acad. Sci. U. ...

https://fa.wikipedia.org/wiki/سرطان_پروستات?match=en

... arachidonate 12-lipoxygenase EC 1.13.11.32: 2-nitropropane dioxygenase EC 1.13.11.33: arachidonate 15-lipoxygenase EC 1.13. ... linoleate 8R-lipoxygenase EC 1.13.11.61: linolenate 9R-lipoxygenase EC 1.13.11.62: linoleate 10R-lipoxygenase EC 1.13.11.63: ... 11.34: arachidonate 5-lipoxygenase EC 1.13.11.35: pyrogallol 1,2-oxygenase EC 1.13.11.36: chloridazon-catechol dioxygenase EC ... arachidonate 8-lipoxygenase EC 1.13.11.41: 2,4'-dihydroxyacetophenone dioxygenase EC 1.13.11.42: deleted EC 1.13.11.43: ...

https://en.wikipedia.org/wiki/List_of_EC_numbers_(EC_1)

Ghosh J, Myers CE (1998). "Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells ... mediates the survival-promoting effects of arachidonate 5-lipoxygenase in prostate cancer cells". Cancer Lett. 336 (1): 185-95 ... Hussey HJ, Tisdale MJ (1996). "Inhibition of tumour growth by lipoxygenase inhibitors". Br. J. Cancer. 74 (5): 683-687. doi: ... Wijkander J, O'Flaherty JT, Nixon AB, Wykle RL (1995). "5-Lipoxygenase products modulate the activity of the 85-kDa ...

https://en.wikipedia.org/wiki/Oxoeicosanoid_receptor_1

... s are synthesized in the cell from arachidonic acid by arachidonate 5-lipoxygenase. The catalytic mechanism involves ... and donated by the 5-lipoxygenase-activating protein (FLAP) to 5-lipoxygenase.[citation needed] 5-Lipoxygenase (5-LO) uses FLAP ... by the enzyme arachidonate 5-lipoxygenase. Leukotrienes use lipid signaling to convey information to either the cell producing ... Recent research points to a role of 5-lipoxygenase in cardiovascular and neuropsychiatric illnesses. Leukotrienes are very ...

https://en.wikipedia.org/wiki/Leukotriene

Arachidonate 5-lipoxygenase has been identified as playing a significant role in the survival of prostate cancer cells. ... In particular, arachidonate 5-lipoxygenase inhibitors produce massive, rapid programmed cell death in prostate cancer cells. ... Ghosh J, Myers CE (October 1998). "Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate ... The 5-lipoxygenase (5-LOX) pathway is implicated in the development and progression of human cancers. 5-LOX, whose crystal ...

https://en.wikipedia.org/wiki/Prostate_cancer

Arachidonate 5-lipoxygenase, B-cell linker, BCAR1, BCR gene, Beta-2 adrenergic receptor, C-Met, CBLB, CD117, CD22, CD28, CDKN1B ... Lepley RA, Fitzpatrick FA (September 1994). "5-Lipoxygenase contains a functional Src homology 3-binding motif that interacts ... "Competitive binding assay of src homology domain 3 interactions between 5-lipoxygenase and growth factor receptor binding ... 15 (13): 1565-72. doi:10.1038/sj.onc.1201518. PMID 9380408. Sahni M, Zhou XM, Bakiri L, Schlessinger J, Baron R, Levy JB ( ...

https://en.wikipedia.org/wiki/GRB2

When acting on GLA, arachidonate 5-lipoxygenase produces no leukotrienes and the conversion by the enzyme of arachidonic acid ... Borage oil contains 20% GLA, evening primrose oil ranges from 8% to 10% GLA, and black-currant oil contains 15-20%. It also ... DGLA itself cannot be converted to LTs but can form a 15-hydroxyl derivative that blocks the transformation of arachidonic acid ...

https://en.wikipedia.org/wiki/Gamma-Linolenic_acid

... is an enzyme that metabolizes an eicosanoid product of arachidonate 5-lipoxygenase (5-LOX), 5(S)-hydroxy-6S,8Z,11Z,14Z- ... A 15-hydroxyicosatetraenoate dehydrogenase metabolizes 15-Hydroxyicosatetraenoic acid (i.e. 15(S)-hydroxy-5Z,8Z,11Z,13E- ... 15(S)-HETE, leukotriene B4, a racemate mixture of 9-HETE, a racemate mixture of 11-HETE, or a 5(S)-hydroxy-6-trans 12 carbon ... to its 15-keto analog, 15-oxo-ETE (see 15-Hydroxyicosatetraenoic acid using NAD+ and NADH rather than NADP+ and NADPH as its co ...

https://en.wikipedia.org/wiki/5-Hydroxyeicosanoid_dehydrogenase

... , also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13 ... that in humans is encoded by the ALOX5 gene.[5] Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. ... "Alox5 - arachidonate 5-lipoxygenase". WikiGenes.. *^ Fahel JS, de Souza MB, Gomes MT, Corsetti PP, Carvalho NB, Marinho FA, de ... arachidonate 5-lipoxygenase activity. • dioxygenase activity. • metal ion binding. • protein binding. • oxidoreductase activity ...

https://en.m.wikipedia.org/wiki/5-Lipoxygenase

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... InChI=1S/C17H14Cl2F2N2O3/c18-11-6-22-7-12(19)15(11)23-16(24)10-3-4-13(26-17(20)21)14(5-10)25-8-9-1-2-9/h3-7,9,17H,1-2,8H2,(H,22 ... 155 (3): 308-15. doi:10.1038/bjp.2008.307. ISSN 1476-5381. PMC 2567892. PMID 18660825.. ...

https://en.wikipedia.org/wiki/Roflumilast

5-Lipoxygenase activating protein/Arachidonate 5-lipoxygenase. *LTA4 hydrolase (B4 synthesis). *LTC4 synthase ... 269 (15): 11269-73. PMID 8157657.. *. Ohishi N, Izumi T, Minami M, Kitamura S, Seyama Y, Ohkawa S, Terao S, Yotsumoto H, Takaku ...

https://en.wikipedia.org/wiki/Leukotriene-A4_hydrolase

Brash A. R., Boeglin W. E., Chang M. S. (Jun 1997)։ «Discovery of a second 15S-lipoxygenase in humans»։ Proc Natl Acad Sci U S ... 5,8,11,14-all-cis-Eicosatetraenoic acid; all-cis-5,8,11,14-Eicosatetraenoic acid; Arachidonate. ... A neglected platelet-derived 12-lipoxygenase product»։ J Chromatogr B 964: 26-40։ PMID 24685839։ doi:10.1016/j.jchromb.2014.03. ... Zhu D, Ran Y (May 2012)։ «Role of 15-lipoxygenase/15-hydroxyeicosatetraenoic acid in hypoxia-induced pulmonary hypertension»։ J ...

https://hy.wikipedia.org/wiki/Արախիդոնաթթու

Other lipoxygenases-8-LO, 12-LO and 15-LO-make other eicosanoid-like products. To act, 5-LO uses the nuclear-membrane enzyme 5- ... Phinney, SD; RS Odin; SB Johnson & RT Holman (March 1, 1990). "Reduced arachidonate in serum phospholipids and cholesteryl ... Lipoxygenase oxidation removes no C=C double bonds, and leads to the LK. After oxidation, the eicosanoids are further modified ... DGLA and EPA compete with AA for access to the cyclooxygenase and lipoxygenase enzymes. So the presence of DGLA and EPA in ...

https://en.wikipedia.org/wiki/Essential_fatty_acid_interactions

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... InChI=1S/C22H30N2O2/c1-26-22(20-10-6-3-7-11-20)18-24-16-14-23(15-17-24)13-12-21(25)19-8-4-2-5-9-19/h2-11,21-22,25H,12-18H2,1H3 ...

https://en.wikipedia.org/wiki/Eprozinol

... arachidonate 5-lipoxygenase) or leukotriene receptor antagonists (cysteinyl leukotriene receptors) and consequently oppose the ... Ottani, Alessandra; Leone, Sheila; Sandrini, Maurizio; Ferrari, Anna; Bertolini, Alfio (February 15, 2006). "The analgesic ... 15] feG has also been shown to decrease circulating neutrophil and eosinophil accumulation, decrease intracellular oxidative ...

https://en.m.wikipedia.org/wiki/Anti-inflammatory

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... The remaining signals correspond to carbons characteristic of the xanthine backbone.[15] ...

https://en.wikipedia.org/wiki/Theophylline

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... 15-17H2,1-4H3/t22-,23-,24-,26+,27+,29+,30-,31-,32+/m0/s1 Y ... Non-corticosteroids with some glucocorticoid activity: 15β- ...

https://en.wikipedia.org/wiki/Ciclesonide

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... 15-6-4-8-26-15/h3-8,11-13,16-17,22H,9-10H2,1-2H3/q+1/t11?,12-,13+,16-,17-/m0/s1 Y ...

https://en.wikipedia.org/wiki/Tiotropium_bromide

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... Taken 15 to 20 minutes ahead of time, these medications can also prevent asthma symptoms triggered by exercise or exposure to ...

https://en.wikipedia.org/wiki/Bronchodilator

Arachidonate 5-lipoxygenase. *Atrophin 1. *BH3 interacting-domain death agonist. *BRAF (gene) ...

https://en.wikipedia.org/wiki/File:Ideogram_house_mouse_chromosome_6.svg

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... InChI=1S/C16H14N2O4/c1-7(2)8-3-4-12-9(5-8)13(19)10-6-11(16(20)21)14(17)18-15(10)22-12/h3-7H,1-2H3,(H2,17,18)(H,20,21) Y ...

https://en.wikipedia.org/wiki/Amlexanox

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... Ipratropium is a derivative of atropine[15] but is a quaternary amine and therefore does not cross the blood-brain barrier, ... InChI=1S/C20H30NO3.BrH/c1-14(2)21(3)16-9-10-17(21)12-18(11-16)24-20(23)19(13-22)15-7-5-4-6-8-15;/h4-8,14,16-19,22H,9-13H2,1-3H3 ... Onset of action is typically within 15 to 30 minutes and lasts for three to five hours.[1] ...

https://en.wikipedia.org/wiki/Ipratropium_bromide

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... 15 (1): 453-456. doi:10.1002/cber.18820150194.. *^ Fischer, Emil (1882). "Über Caffein, Theobromin, Xanthin und Guanin". Justus ...

https://en.wikipedia.org/wiki/Theobromine

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... 15][16][17][18][19][20][21][22][23][24][25][26] Xanthines are also found very rarely as constituents of nucleic acids. ...

https://en.wikipedia.org/wiki/Methylxanthines

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... Human trials are planned, starting with Parkinson's disease patients.[15] The researchers conducted the experiment with ...

https://en.wikipedia.org/wiki/Montelukast

5,8,11,14-all-cis-Eicosatetraenoic acid; all-cis-5,8,11,14-Eicosatetraenoic acid; Arachidonate ... The enzyme 12-lipoxygenase (ALOX12) metabolizes arachidonic acid to 12-hydroperoxyeicosatetraenoic acid (12-HPETE) which may ... The enzyme 5-lipoxygenase metabolizes arachidonic acid to 5-hydroperoxyicosatetraenoic acid (5-HPETE), which in turn is ... A neglected platelet-derived 12-lipoxygenase product". J Chromatogr B. 964: 26-40. doi:10.1016/j.jchromb.2014.03.015. PMID ...

https://en.wikipedia.org/wiki/Arachidonic_acid

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... 43 (5): 604-15. doi:10.1093/ageing/afu096. PMID 25038833.. *^ Andre, L; Gallini, A; Montastruc, F; Montastruc, JL; Piau, A; ... Falk, N; Cole, A; Meredith, TJ (15 March 2018). "Evaluation of Suspected Dementia". American Family Physician. 97 (6): 398-405 ...

https://en.wikipedia.org/wiki/Anticholinergics

Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... Beclometasone is mainly a glucocorticoid.[1] Glucocorticoids are corticosteroids that bind to the glucocorticoid receptor[15] ... 15,16,17-dodecahydro-3H-cyclopenta[a]phenanthren-17-yl propionate ... Non-corticosteroids with some glucocorticoid activity: 15β- ...

https://en.wikipedia.org/wiki/QVAR

Main article: Arachidonate 5-lipoxygenase inhibitor. Drugs that inhibit the 5-lipoxygenase enzyme will inhibit the synthetic ... 1. Arachidonate 5-lipoxygenase ...Specific function: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a ... arachidonate 5-lipoxygenase) or leukotriene receptor antagonist (cysteinyl leukotriene receptors) and consequently opposes the ... Leukotriene receptor antagonists, such as montelukast, zafirlukast, and pranlukast,[2] and 5-lipoxygenase inhibitors, like ...

https://en.wikipedia.org/wiki/Antileukotriene

Arachidonate + O2 = 5Z,8Z,11Z,13E-15S-15-hydroperoxyicosa-5,8,11,13-tetraenoate. UniProt ... Hydropathy has been calculated using a sliding window of 15 residues and summing up scores from standard hydrophobicity tables ... Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. ...

http://www.rcsb.org/pdb/protein/P27479

1988 Jan 15;150(1):376-83. Comparative Study; Research Support, Non-U.S. Govt; Research Support, U.S. Govt, Non-P.H.S.; ... Arachidonate 15-lipoxygenase from human eosinophil-enriched leukocytes: partial purification and properties.. Sigal E1, ... Arachidonate 15-lipoxygenase was purified from human eosinophil-enriched leukocytes after showing that 15-lipoxygenase activity ... In addition, ATP is shown to inhibit, and phosphatidylcholine is shown to stimulate, 15-lipoxygenase, suggesting a regulatory ...

https://www.ncbi.nlm.nih.gov/pubmed/3337718

Here, we determined that the gene encoding arachidonate 15-lipoxygenase (Alox15/15-LO) is essential for the survival of ... Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival. ... Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival. ... In human CML cell lines and CD34+ cells, knockdown of Alox15 or inhibition of 15-LO dramatically reduced survival. Loss of ...

https://www.jci.org/articles/view/66129/figure/8

Arachidonate 15-lipoxygenase is required for chronic myeloid leukemia stem cell survival. Yaoyu Chen,1 Cong Peng,1 Sheela A. ... Alox15 encodes arachidonate 15-lipoxygenase (15-LO). Compared with Alox5, which we identified previously (19), Alox15 has ... Regulation of bone mass in mice by the lipoxygenase gene Alox15. Science. 2004;303(5655):229-232.. View this article via: ... Here, we determined that the gene encoding arachidonate 15-lipoxygenase (Alox15/15-LO) is essential for the survival of ...

https://www.jci.org/articles/view/66129

PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. ... PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. ... LipoxygenaseInterPro annotation. ,p>Information which has been generated by the UniProtKB automatic annotation system, without ... arachidonate 15-lipoxygenaseImported. ,p>Information which has been imported from another database using automatic procedures ...

http://www.uniprot.org/uniprot/A0A1U7SWG9

Compare and order Arachidonate 15-Lipoxygenase ELISA Kits. View citations, images, detection ranges, sensitivity, prices and ... 12S-lipoxygenase , arachidonate 12-lipoxygenase, 12S-type , Arachidonate 15-lipoxygenase , erythroid cell-specific 15- ... arachidonate omega-6 lipoxygenase , arachidonate 15-lipoxygenase , 12-LOX , arachidonate 12-lipoxygenase, leukocyte-type , 12/ ... Images for product: Arachidonate 15-Lipoxygenase (ALOX15) ELISA Kit Diagramm of the ELISA kit to detect Human 15-LOwith the ...

https://www.antikoerper-online.de/regulation-of-actin-filament-polymerization-pathway-56/alox15-elisa-kit-13142/

We have shown previously that arachidonate 15-lipoxygenase type B (ALOX15B) is more highly expressed in human atherosclerotic ... Furstenberger G,, Marks F,, Krieg P, (Year: 2002) Arachidonate 8(S)-lipoxygenase. Prostaglandins Other Lipid Mediat68-69: 235- ... as well as lipoxygenases, which oxygenate polyunsaturated fatty acids to proinflammatory mediators [4]. Arachidonate 15- ... Identification of amino acid determinants of the positional specificity of mouse 8S-lipoxygenase and human 15S-lipoxygenase-2. ...

http://www.biomedsearch.com/nih/Arachidonate-15-lipoxygenase-type-B/22912809.html

OMIM: ARACHIDONATE 15-LIPOXYGENASE; ALOX15*Gene Ontology: Alox15 *Mouse Phenome DB: Alox15 *UCSC: Chr.11:70,344,147-70,352,031 ... arachidonate 15-lipoxygenase. Synonyms: 12-LO, Alox12l, L-12LO. Gene nomenclature, locus information, and GO, OMIM, and PMID ... Vega: OTTMUSG6044 (Alox15, arachidonate 15-lipoxygenase)*CCDS: 24944, 24944.1* ...

https://www.komp.org/geneinfo.php?MGI_Number=87997

Bermudez, AF, Coles, B, Coffey, M and ODonnell, V (2003) Protein kinase C regulates 15-lipoxygenase oxidation of membrane- ... bound arachidonate in human monocytes In: 10th Annual Meeting of the Society-for-Free-Radical-Biology-and-Medicne, 2003-11-20 ... Protein kinase C regulates 15-lipoxygenase oxidation of membrane-bound arachidonate in human monocytes ...

http://epubs.surrey.ac.uk/824040/

All structured data from the main and property namespace is available under the Creative Commons CC0 License; text in the other namespaces is available under the Creative Commons Attribution-ShareAlike License; additional terms may apply. By using this site, you agree to the Terms of Use and Privacy Policy. ...

https://www.wikidata.org/wiki/Q3621201

Arachidonate 15-Lipoxygenase, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The ... Arachidonate 12-Lipoxygenase, Leukocyte-Type 3 4 * Arachidonate Omega-6 Lipoxygenase 3 4 ... 5- and 12-Lipoxygenase inhibitor. 491-67-8. BAY-X 1005. Orally active 5-lipoxygenase activating protein (FLAP) inhibitor. ... Lipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty ...

https://www.genecards.org/cgi-bin/carddisp.pl?gene=ALOX15

arachidonate 15-lipoxygenase. Enable Javascript to view the expand/collapse boxes.. Open All Close All ... This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty ... Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. ...

https://ghr.nlm.nih.gov/gene/ALOX15

Arachidonate 15-Lipoxygenase Pseudogene 2, including: function, proteins, disorders, pathways, orthologs, and expression. ... ALOX15P2 (Arachidonate 15-Lipoxygenase Pseudogene 2) is a Pseudogene. Additional gene information for ALOX15P2 Gene. ...

https://www.genecards.org/cgi-bin/carddisp.pl?gene=ALOX15P2

Arachidonate 15-lipoxygenase Homo sapiens 0.883 CHEMBL1287622 Lethal(3)malignant brain tumor-like protein 1 Homo sapiens 0.852 ...

https://www.ebi.ac.uk/chembl/compound/inspect/CHEMBL15841

Arachidonate 15-lipoxygenase Homo sapiens 0.902 CHEMBL2392 DNA polymerase beta Homo sapiens 0.811 ... 15-hydroxyprostaglandin dehydrogenase [NAD+] Homo sapiens 0.933 CHEMBL6110 Thioredoxin glutathione reductase Schistosoma ...

https://www.ebi.ac.uk/chembldb/index.php/compound/inspect/CHEMBL416

12S-lipoxygenase. Arachidonate 12-lipoxygenase, 12S-type. arachidonate (12S)-lipoxygenase. arachidonate (15S)-lipoxygenase. ... ALOX12 arachidonate 12-lipoxygenase, 12S type [Homo sapiens] ALOX12 arachidonate 12-lipoxygenase, 12S type [Homo sapiens]. Gene ... linoleate 13S-lipoxygenase. lipoxin synthase 12-LO. platelet 12-LOX. platelet-type 12-lipoxygenase. platelet-type lipoxygenase ... arachidonate 12-lipoxygenase, 12S typeprovided by HGNC. Primary source. HGNC:HGNC:429 See related. Ensembl:ENSG00000108839 MIM: ...

https://www.ncbi.nlm.nih.gov/gene/239

Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells.. Authors: ... the most abundant arachidonate-metabolizing LOX in adult human prostate and a negative cell-cycle regulator in normal human ... the most abundant arachidonate (AA)-metabolizing enzyme expressed in adult human prostate, is a negative cell-cycle regulator ... is the major mammalian lipoxygenase expressed in normal human adult prostate and its expression is decreased or lost in high- ...

https://www.pubfacts.com/detail/11839751/Evidence-that-arachidonate-15-lipoxygenase-2-is-a-negative-cell-cycle-regulator-in-normal-prostate-e

https://en.wikipedia.org/wiki/13-oxoODE

Arachidonate 5-lipoxygenase. MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGA.... unknown. Aryl hydrocarbon receptor. ... InChI=1S/C14H12O3/c15-12-5-3-10(4-6-12)1-2-11-7-13(16)9-14(17)8-11/h1-9,15-17H/b2-1+. ...

http://www.rcsb.org/ligand/STL

Comparison of relative arachidonate 15-lipoxygenase activities in different types of human cell reveals striking differences in ... Holtzman, M. J. ; Pentland, A. ; Baenziger, N. L. ; Hansbrough, J. R. / Heterogeneity of cellular expression of arachidonate 15 ... keywords = "Cyclooxygenase, Leukotriene, Lipoxygenase, Prostaglandin",. author = "Holtzman, {M. J.} and A. Pentland and ... Heterogeneity of cellular expression of arachidonate 15-lipoxygenase : implications for biological activity. / Holtzman, M. J. ...

https://profiles.wustl.edu/en/publications/heterogeneity-of-cellular-expression-of-arachidonate-15-lipoxygen

arachidonate 15-lipoxygenase. 53. 5. 6. 12. Sequence references in MGI J:259852 Mouse Genome Informatics and the WTSI Mouse ...

http://www.informatics.jax.org/sequence/MGP_C57BL6NJ_G0018983

Arachidonate 15-lipoxygenase. ALOX15. 34636_at. nc. Heparan sulfate (glucosamine) 3-O-sulfotransferase 1. HS3ST1. 41555_at. nc ...

http://www.jimmunol.org/content/181/1/698/tab-figures-data

AMP-activated protein kinase suppresses arachidonate 15-lipoxygenase expression in interleukin 4-polarized human macrophages. J ... ACLY, ATP-citrate lyase; ALOX15, arachidonate 15-Lipoxygenase; BMDM, bone marrow-derived macrophage; IL, interleukin; MDM, ... 15. Lee JV, Carrer A, Shah S, Snyder NW, Wei S, Venneti S, et al. Akt-dependent metabolic reprogramming regulates tumor cell ... the role of ACLY in IL-4-stimulated human macrophage polarization we initially analyzed mRNA expression of arachidonate 15- ...

https://www.frontiersin.org/articles/10.3389/fimmu.2018.02858/full

8-lipoxygenase (S-type). K19246 arachidonate 15-lipoxygenase. ... arachidonate + O2 = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5 ... Positional specificity of a reticulocyte lipoxygenase. Conversion of arachidonic acid to 15-S-hydroperoxy-eicosatetraenoic acid ... Metabolism of polyunsaturated fatty acids by an (n - 6)-lipoxygenase associated with human ejaculates. ... arachidonate 15-lipoxygenase. K08022 arachidonate 15-lipoxygenase (second type) / ...

https://www.genome.jp/dbget-bin/www_bget?enzyme+1.13.11.33

ALOX15; arachidonate 15-lipoxygenase [KO:K00460] [EC:1.13.11.33]. 10162 LPCAT3; lysophosphatidylcholine acyltransferase 3 [KO: ... Nat Rev Drug Discov 15:348-66 (2016). DOI:10.1038/nrd.2015.6 ...

https://www.genome.jp/dbget-bin/www_bget?hsa04216

Mouse monoclonal 15 Lipoxygenase 1 antibody [7H6]. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Human. Immunogen ... Also acts on C-12 of arachidonate as well as on linoleic acid. ... Belongs to the lipoxygenase family.. Contains 1 lipoxygenase ... Primary - Mouse Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919) WB, ICC/IF, Flow Cyt, IHC-P ... All lanes : Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919) at 1/2000 dilution. Lane 1 : pCMV6-ENTRY control-transfected HEK- ...

https://www.abcam.com/15-lipoxygenase-1-antibody-7h6-ab230919.html

ALOX12; arachidonate 12-lipoxygenase, 12S type [KO:K00458] [EC:1.13.11.31]. 242 ALOX12B; arachidonate 12-lipoxygenase, 12R type ... ALOX15B; arachidonate 15-lipoxygenase, type B [KO:K08022] [EC:1.13.11.- 1.13.11.33]. ... Rabbit aorta converts 15-HPETE to trihydroxyeicosatrienoic acids: potential role of cytochrome P450. ... Identification of the 11,14,15- and 11,12, 15-trihydroxyeicosatrienoic acids as endothelium-derived relaxing factors of rabbit ...

https://www.genome.jp/dbget-bin/www_bget?pathway+hsa00590

Gallic acid esters possessing a varying chain length of their alcohol moiety were tested for their inhibitory potencies on 15- ... lipoxygenase from rabbit reticulocytes and prostaglandin H synthase from sheep vesicular glands. Octyl gallate and decyl gal ... Arachidonate 15-Lipoxygenase / antagonists & inhibitors*. Cyclooxygenase Inhibitors*. Exocrine Glands / enzymology*. Gallic ... 0/Cyclooxygenase Inhibitors; 0/Prostaglandins H; 149-91-7/Gallic Acid; EC 1.13.11.33/Arachidonate 15-Lipoxygenase ...

http://www.biomedsearch.com/nih/Inhibition-rabbit-erythroid-15-lipoxygenase/1906619.html

2004) The opportunistic pathogen Pseudomonas aeruginosa carries a secretable arachidonate 15-lipoxygenase. Proc Natl Acad Sci ... such as 5-lipoxygenase or 15-lipoxygenase (63). Products of lipoxygenases have profound and distinct effects on the outcome of ... Plots of kobs of MtAhpE oxidation by LAOOH (A), OAOOH (B), 5(S)-HpETE (C), 15(S)-HpETE (D), and H2O2 (E). kox, second-order ... Effects of increasing concentration of XfOhr on MtAhpE oxidation by LAOOH (F), OAOOH (G), 5(S)-HpETE (H), and 15(S)-HpETE (I ...

https://www.pnas.org/content/114/2/E132

Arachidonate 5-lipoxygenase. Molecular Weight. 77982.595 Da. References. *Ibeas Bih C, Chen T, Nunn AV, Bazelot M, Dallas M, ... Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive ... Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some ... Details15. Transient receptor potential cation channel subfamily V member 1. Kind. Protein. Organism. Humans. Pharmacological ...

https://www.drugbank.ca/drugs/DB14011

Here, we determined that the gene encoding arachidonate 15-lipoxygenase (Alox15/15-LO) is essential for the survival of leukemia stem cells (LSCs) in a murine model of BCR-ABL-induced chronic myeloid leukemia (CML). (jci.org)
In human CML cell lines and CD34 + cells, knockdown of Alox15 or inhibition of 15-LO dramatically reduced survival. (jci.org)
ALOX15 (Arachidonate 15-Lipoxygenase) is a Protein Coding gene. (genecards.org)
15-lipoxygenase 1 ( ALOX15 ), while best known for converting the 20 carbon polyunsaturated fatty acid , arachidonic acid , into a series of 15-hydroxylated arachidonic acid metabolites (see 15-hydroxyicosatetraenoic acid ), actually prefers as its substrate the 18 carbon polyunsaturated fatty acid, linoleic acid , over arachidonic acid, converting it to 13-hydroperoxy-9 Z ,11 E -octadecadienoic acid (13-HpODE). (wikipedia.org)
15-Lipoxygenase antibody LS-C173699 is an unconjugated mouse monoclonal antibody to human 15-Lipoxygenase (ALOX15). (lsbio.com)
LS-F23767 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human ALOX15 / 15-Lipoxygenase in samples of Plasma and Serum. (lsbio.com)
It is based upon a Sandwich assay principle and can be used to detect levels of ALOX15 / 15-Lipoxygenase as low as 37.5 picograms per milliliter. (lsbio.com)
The M1 macrophages exhibited preferential induction of arachidonate 5-lipoxygenase activating protein (ALOX5AP), whereas M2 macrophages had a high-level expression of arachidonate 15-lipoxygenase (ALOX15). (cdc.gov)

Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. (rcsb.org)
In addition, ATP is shown to inhibit, and phosphatidylcholine is shown to stimulate, 15-lipoxygenase, suggesting a regulatory role for these compounds in the lipoxygenation of arachidonic acid. (nih.gov)
Conversion of arachidonic acid to 15-S-hydroperoxy-eicosatetraenoic acid. (genome.jp)
Arachidonic acid-15-lipoxygenase from rabbit peritoneal polymorphonuclear leukocytes. (genome.jp)
Lipoxygenases (LOX) are a heterogeneous family of enzymes that catalyze the insertion of molecular oxygen into polyunsaturated fatty acids (PUFAs), such as arachidonic acid (AA) and linoleic acid (LA), into the corresponding hydroperoxyl derivates, which can be potent inflammatory and prooxidant mediators [ 1 , 2 ]. (hindawi.com)
In mammals, the hydroperoxidation of arachidonic acid by lipoxygenases leads to the formation of leukotrienes and lipoxins, compounds that mediate inflammatory responses. (elsevier.com)
It was first found as a product of arachidonic acid metabolism made by human and bovine platelets through their 12S-lipoxygenase (i.e. (wikipedia.org)
Mouse e-12LO metabolizes arachidonic acid predominantly to 12(S)-HETE and to a lesser extent 15(S)-HETE. (wikipedia.org)
The third pathway for metabolizing arachidonic acid, the lipoxygenase pathway catalyzes the incorporation of one oxygen molecule into polyunsaturated fatty acids to yield a 1-hydroperoxy-2, 4-trans, cis-pentadiene product [ 14 - 16 ]. (hindawi.com)
Hepoxilins are biologically relevant eicosanoids formed via the 12-lipoxygenase pathway of the arachidonic acid cascade. (semanticscholar.org)

15-Lipoxygenase 2 (15-LOX2), the most abundant arachidonate (AA)-metabolizing enzyme expressed in adult human prostate, is a negative cell-cycle regulator in normal human prostate epithelial cells. (pubfacts.com)
Arachidonate 5-lipoxygenase, also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13.11.34) that in humans is encoded by the ALOX5 gene. (wikipedia.org)
The structure of soybean lipoxygenase-1 solved to 2.6 angstrom resolution shows that the enzyme has two domains: a 146-residue β barrel and a 693-residue helical bundle. (elsevier.com)
In both plant and mammalian enzymes, the N-terminal domain contains an eight-stranded antiparallel β-barrel, but in the soybean lipoxygenases this domain is significantly larger than in the rabbit enzyme. (chemeurope.com)

This gene encodes a member of the lipoxygenase family of proteins. (genecards.org)
The ALOX5 gene, which occupies 71.9 kilobase pairs (kb) on chromosome 10 (all other human lipoxygenases are clustered together on chromosome 17), is composed of 14 exons divided by 13 introns encoding the mature 78 kilodalton (kD) ALOX5 protein consisting of 673 amino acids. (wikipedia.org)
This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. (bio-rad.com)
In humans, Arachidonate 12-lipoxygenase (12-LO, 12-LOX, ALO12, or platelet type 12-lipoxygenase) is encoded by the ALOX12 gene and expressed primarily in platelets and skin. (wikipedia.org)
The results of gene expression profiles from buccal ( n = 10) and nasal ( n = 15) epithelial cells indicated that many of the smoking-related changes in the bronchial epithelium were also present in buccal and nasal epithelium. (aacrjournals.org)
Arachidonate 15-lipoxygenase B (676 aa, ~76 kDa) is encoded by the human ALOX15B gene. (semanticscholar.org)

We have shown previously that arachidonate 15-lipoxygenase type B (ALOX15B) is more highly expressed in human atherosclerotic lesions than in healthy arteries. (biomedsearch.com)

15-Lipoxygenase 2 (15-LOX2) is the major mammalian lipoxygenase expressed in normal human adult prostate and its expression is decreased or lost in high-grade prostate intraepithelial neoplasia (HGPIN) and prostate cancer (PCa). (pubfacts.com)
Soybean lipoxygenase-1 is a single-chain, 839-residue protein closely related to mammalian lipoxygenases. (elsevier.com)
Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)
Mammalian lipoxygenases possess regiospecificity during interaction with substrate, and on this basis have been designated as arachidonate 5-, 12-, and 15-lipoxygenase (5-LOX, 12-LOX, and 15-LOX) [ 14 - 16 ]. (hindawi.com)
Conformational flexibility in mammalian 15S-lipoxygenase: Reinterpretation of the crystallographic data. (semanticscholar.org)
Mammalian arachidonate 5-lipoxygenase ( EC 1.13.11.34 IPR001885 ). (chemeurope.com)
Mammalian erythroid cell-specific 15-lipoxygenase ( EC 1.13.11.33 IPR001885 ). (chemeurope.com)

Bermudez, AF , Coles, B , Coffey, M and O'Donnell, V (2003) Protein kinase C regulates 15-lipoxygenase oxidation of membrane-bound arachidonate in human monocytes In: 10th Annual Meeting of the Society-for-Free-Radical-Biology-and-Medicne, 2003-11-20 - 2003-11-24, SEATTLE, WASHINGTON. (surrey.ac.uk)
The protein level and enzymatic activity of 15-LOX2 have been shown to be down-regulated in prostate cancers compared with normal and benign prostate tissues. (pubfacts.com)
Recombinant full length protein corresponding to Human 15 Lipoxygenase 1. (abcam.com)
5 One lipoxin in particular, LXA4, controls leukocyte responses via its own G-protein-coupled receptor 4 which also engages 15-epi-LXA4. (springer.com)
12 13 14 15 16 In the tree shrew, regulation of collagen content occurs at both the mRNA and protein levels. (arvojournals.org)

Sub-primate mammals, such as the mouse, rat, rabbit, cow, and pig, express platelet type 12-lipoxygenase but also a leukocyte type 12-lipoxygenase (also termed 12/15-lipoxygenase, 12/15-LOX or 12/15-LO) which is an ortholog of, and metabolically equivalent to, human 15-LO-1 in that it forms predominantly 15(S)-HpETE with 12(S)-HpETE as a minor product. (wikipedia.org)
The rat leukocyte-type 12-lipoxygenase exhibits an intrinsic hepoxilin A3 synthase activity. (semanticscholar.org)

Anti-Human 15-LO antibody was pre-coated onto 96-well plates. (biobool.com)
And the biotin conjugated anti-Human 15-LO antibody was used as detection antibodies. (biobool.com)
Product Description specifical The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human 15-LO. (biobool.com)
Then a biotinylated detection antibody specific for Human 15-LO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. (biobool.com)
Only those wells that contain Human 15-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. (biobool.com)

Lipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty acid hydroperoxidases. (genecards.org)
Arachidonate 12-lipoxygenase and 12-hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer. (nih.gov)
Gallic acid esters possessing a varying chain length of their alcohol moiety were tested for their inhibitory potencies on 15-lipoxygenase from rabbit reticulocytes and prostaglandin H synthase from sheep vesicular glands. (biomedsearch.com)
Because enhanced LX production was associated with an increased anti-inflammatory status of 15-LO TG rabbits, a stable analog of 5 S ,6 R ,15 S -trihydroxyeicosa-7 E ,9 E ,11 Z ,13 E -tetraenoic acid (LXA 4 ) was applied to the gingival crevice subject to periodontitis. (jimmunol.org)
To evaluate this, 15-hydroperoxy-eicosatetraenoic acid (15s-HpETE), a lipid peroxide, was employed to investigate its effect on PTEN using molecular and cellular-based assays. (hindawi.com)
15-LOX metabolizes AA to form 15(s)-hydroperoxyeicosatetraenoic acid (15s-HpETE), the oxidative precursor of 15-hydroxyeicosatetraenoic acid (15s-HETE). (hindawi.com)
Mice also express an epidermal type 15-lipoxygenase (e-12LO) which has 50.8% amino acid sequence identity to human 15-LOX-2 and 49.3% sequence indetity to mouse Arachidonate 8-lipoxygenase. (wikipedia.org)
CREB-mediated IL-6 expression is required for 15(S)-hydroxyeicosatetraenoic acid-induced vascular smooth muscle cell migration. (semanticscholar.org)
Six key genes in lipid metabolism: fatty acid desaturase 2, phospholipase A2 group IVA, arachidonate 15-lipoxygenase, phosphatidylethanolamine N -methyltransferase, monoglyceride lipase, and glycerol-3-phosphate acyltransferase, were expressed in opposing direction between subgroups. (biomedcentral.com)

Probing the Electrostatic and Steric Requirements for Substrate Binding in Human Platelet-Type 12-Lipoxygenase. (nih.gov)

15-Lipoxygenase (LO) plays a central role in the "class switch" of eicosanoid mediator biosynthesis from leukotrienes to lipoxins, initiating the active resolution of inflammation. (ersjournals.com)
LY293111 was also known to have inhibitory effects on 5-lipoxygenase, which is upstream of the production of leukotrienes. (hindawi.com)
In mammals a number of lipoxygenases isozymes are involved in the metabolism of prostaglandins and leukotrienes [2] . (chemeurope.com)

The present study shows the first binding model of amentoflavone with few selected human proteins (α-glucosidase, tyrosinase and 15-lipoxygenase) as validated therapeutic targets using computational procedures. (alliedacademies.org)
Results obtained divulged amentoflavone interference with the metal ions within the active site of tyrosinase and lipoxygenase as part of its inhibitory mechanism against the proteins. (alliedacademies.org)

Arachidonate 15-lipoxygenase type B knockdown leads to reduced lipid accumulation and inflammation in atherosclerosis. (biomedsearch.com)
In control m/m mice, the rosiglitazone-induced analgesic effects were reversed by knockdown with arachidonate 5-lipoxygenase small interfering RNA, but these were restored by addition of RvD1. (asahq.org)

Arachidonate 15-lipoxygenase from human eosinophil-enriched leukocytes: partial purification and properties. (nih.gov)
Arachidonate 15-lipoxygenase was purified from human eosinophil-enriched leukocytes after showing that 15-lipoxygenase activity was 100-fold greater in eosinophils than in neutrophils. (nih.gov)
Diagramm of the ELISA kit to detect Human 15-LOwith the optical density on the x-axis and the concentration on the y-axis. (antikoerper-online.de)
Biosynthesis of the Maresin Intermediate, 13S,14S-Epoxy-DHA, by Human 15-Lipoxygenase and 12-Lipoxygenase and Its Regulation through Negative Allosteric Modulators. (nih.gov)
15-Lipoxygenase 2 (15-LOX2) is a recently cloned human lipoxygenase that shows tissue-restricted expression in prostate, lung, skin, and cornea. (pubfacts.com)
Cell-autonomous induction of functional tumor suppressor 15-lipoxygenase 2 (15-LOX2) contributes to replicative senescence of human prostate progenitor cells. (pubfacts.com)
15-lipoxygenase 2 (15-LOX2) is a functional tumor suppressor that regulates human prostate epithelial cell differentiation, senescence, and growth (size). (pubfacts.com)
Comparison of relative arachidonate 15-lipoxygenase activities in different types of human cell reveals striking differences in levels of product generation. (wustl.edu)
Cyclic adenosine 3',5'-monophosphate inhibits the availability of arachidonate to prostaglandin synthetase in human platelet suspensions. (wikipathways.org)
Both 15s-HpETE and 15s-HETE were shown to inhibit the growth of cultured human chronic myelogenous leukemia K562 cells by a mechanism associated with reactive oxygen species (ROS) [ 11 , 14 , 15 ]. (hindawi.com)
Mounting evidence suggests that lipoxygenase (LO)-catalyzed products have a profound influence on the development and progression of human cancers. (aacrjournals.org)
Sub-human primates, although not extensively examined, appear to have 12-lipoxygenase expression patterns that resemble those of sub-primate mammals or humans depending on the closeness of there genetic relateness to these species. (wikipedia.org)
Intended Use Human 15-LO ELISA Kit allows for the in vitro quantitative determination of 15-LO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids. (biobool.com)
Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human 15-LO can be calculated. (biobool.com)
Inquiry About Human 15-LO ELISA Kit If you hope to order it or contact us directly, please contact us via [email protected] (biobool.com)
The RLU value is positively associated with the concentration of Human 15-LO. (biobool.com)
You can calculate the concentration of Human 15-LO in the samples by comparing the RLU value of the samples to the standard curve. (biobool.com)
Reduction of isoforms of 15-lipoxygenase (15-LOX)-1 and 15-LOX-2 in human breast cancer. (semanticscholar.org)
11 In this study, we investigated whether stable analogs of native LXA4 and aspirin-triggered 15-epi-LXA4 are active within the microenvironment of human whole blood, and studied the cellular mechanisms that account for their novel inhibitory actions in leukocyte trafficking. (springer.com)
The mechanistic significance of 15-LOX-1 expression loss in human cancers to terminal cell differentiation suppression is unknown. (elsevier.com)
In a screen of 128 cancer cell lines representing more than 20 types of human cancer, we found that 15-LOX-1 mRNA expression levels were markedly lower than levels in terminally differentiated cells. (elsevier.com)
Relative expression levels of 15-LOX-1 (relative to the level in terminally differentiated primary normal human-derived bronchial epithelial cells) were lower in79%of the screened cancer cell lines than relative expression levels of p16 (INK4A), which promotes terminal cell differentiation and is considered one of the most commonly lost tumor suppressor genes in cancer cells. (elsevier.com)
15-LOX-1 expression levels were lower in human tumors than in paired normal lung epithelia. (elsevier.com)

Positional specificity of a reticulocyte lipoxygenase. (genome.jp)
Octyl gallate and decyl gallate proved to be the most powerful inhibitors of both enzymes showing concentrations of half-inhibition of about 0.25 mumol/l for the reticulocyte lipoxygenase and of about 25 mumol/l for the prostaglandin H synthase. (biomedsearch.com)

Moreover, chemical inhibition of 15-LO function impaired LSC function and attenuated CML in mice. (jci.org)
Inhibition of rabbit erythroid 15-lipoxygenase and sheep vesicular gland prostaglandin H synthase by gallic esters. (biomedsearch.com)

J Biol Chem, 2020 May 15. (nih.gov)

Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. (wikipedia.org)

The crystal structures of soybean and rabbit lipoxygenases are known. (chemeurope.com)
Two cavities in the major domain of soybean lipoxygenase-1 (cavities I and II) extend from the surface to the active site. (chemeurope.com)
In soybean lipoxygenase-3 there is a third cavity which runs from the iron site to the interface of the β-barrel and catalytic domains. (chemeurope.com)

Lipoxygenases are dioxygenases that contain a nonheme iron and are present in many animal cells. (elsevier.com)
15-lipoxygenase (15-LOX) belongs to the structurally and functionally related nonheme iron dioxygenases family. (semanticscholar.org)

Separate lines of research have identified the 5-lipoxygenase/leukotriene B 4 receptor pathway and the PPAR pathway as potential targets for prevention or treatment of this disease. (hindawi.com)

Products of arachidonate 15-lipoxygenases (LO) types I and II display both beneficial roles, such as lipoxins (LXs) that stereoselectively signal counterregulation, as well as potential deleterious actions (i.e., nonspecific phospholipid degradation). (jimmunol.org)
3 Lipoxins are produced from arachidonate by the combined actions of 5-lipoxygease and 12- or 15-lipoxygenase (for review see reference 4). (springer.com)
C.N. Serhan, Lipoxins and novel aspirin-triggered 15-epi-lipoxins (ATL). (springer.com)

Insights into interaction profile and inhibitory potential of amentoflavone with α-glucosidase, tyrosinase and 15-lipoxygenase as validated therapeutic targets. (alliedacademies.org)
The predicted inhibitory potential of the biflavonoid against 15-lipoxygenase correlates with IC50 value (0.04 µM). (alliedacademies.org)

Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells. (pubfacts.com)
We report the cloning and functional characterization of 15-LOX2 and its three splice variants (termed 15-LOX2sv-a, 15-LOX2sv-b, and 15-LOX2sv-c) from primary prostate epithelial cells. (pubfacts.com)
In histologically normal airway epithelial cells, smoking causes a range of abnormalities, including P53 mutations ( 15 ), changes in promoter methylation ( 16 , 17 ), and allelic loss ( 18 ). (aacrjournals.org)
15-LOX-1 was expressed during terminal differentiation in three-dimensional air-liquid interface cultures, and 15-LOX-1 expression and terminal differentiation occurred in immortalized nontransformed bronchial epithelial but not in lung cancer cell lines. (elsevier.com)

Plant lipoxygenases ( EC 1.13.11.12 IPR001246 ). (chemeurope.com)

Discovery of a second 15S-lipoxygenase in humans. (semanticscholar.org)

Immunocytochemistry and biochemical fractionation reveal that 15-LOX2 is expressed at multiple subcellular locations, including cytoplasm, cytoskeleton, cell-cell border, and nucleus. (pubfacts.com)

Subcellular localization and tumor-suppressive functions of 15-lipoxygenase 2 (15-LOX2) and its splice variants. (pubfacts.com)

In this study, we examined transgenic (TG) rabbits overexpressing 15-LO type I and their response to inflammatory challenge. (jimmunol.org)
These results indicate that overexpression of 15-LO type I and LXA 4 is associated with dampened PMN-mediated tissue degradation and bone loss, suggesting that enhanced anti-inflammation status is an active process. (jimmunol.org)

In rabbit lipoxygenase, this Asn residue is replaced with His which coordinates the iron via N δ atom. (chemeurope.com)

Omega 3 fatty acids inhibit tumor growth by a number of mechanisms including suppression of COX-2 expression and, for EPA at least, the alternative substrate produces different cyclooxygenase (PGE 3 ) and lipoxygenase (LTB 5 ) products that have anti-inflammatory and anticancer effects. (hindawi.com)

Most notably, 5-lipoxygenase (LO) pathway products including LTC 4 and -D 4 , the slow-reacting substances of anaphylaxis, are considered causative agents in airway allergic responses, because they are potent bronchoconstrictors and inducers of leakage permeability changes ( 2 , 3 ). (jimmunol.org)

glucosidase, tyrosinase and 15-lipoxygenase as validated therapeutic targets. (alliedacademies.org)
Our findings indicate that 15-LOX-1 downregulation in cancer cells is an important mechanism for terminal cell differentiation dysregulation and support the potential therapeutic utility of 15-LOX-1 reexpression to inhibit tumorigenesis. (elsevier.com)

The iron atom in lipoxygenases is bound by four ligands, three of which are histidine residues [4] . (chemeurope.com)

Western blotting with multiple primary prostate cell strains and prostate cancer cell lines reveals that the expression of 15-LOX2 is lost in all prostate cancer cell lines, accompanied by decreased enzymatic activity revealed by liquid chromatography/tandem mass spectrometry analyses. (pubfacts.com)

Short hairpin RNA-mediated downregulation of 15- LOX-1 in Caco-2 cells blocked enterocyte-like differentiation, disrupted tight junction formation, and blocked E-cadherin and ZO-1 localization to the cell wall membrane. (elsevier.com)

The biological function of 15-LOX2 and the role of loss of 15-LOX2 expression in prostate tumorigenesis, however, remain unknown. (pubfacts.com)
Further experiments show that the loss of 15-LOX2 expression results from transcriptional repression caused by mechanism(s) other than promoter hypermethylation or histone deacetylation. (pubfacts.com)
and 3) restoration of 15-LOX2 expression in prostate cancer cells partially inhibits cell cycle progression. (pubfacts.com)
Impoverished 15-LO2 expression and depression of the LXA 4 /LTB 4 ratio are observed in paediatric CF BAL. (ersjournals.com)
15-LOX-1 episomal expression in Caco-2 and HT-29 colon cancer cells induced differentiation. (elsevier.com)

Exp Ther Med 15:5359-5364 (2018). (abcam.com)

Taken together, these results suggest that 15-LOX2 could be a suppressor of prostate cancer development, which functions by restricting cell cycle progression. (pubfacts.com)