Enzymes catalyzing the oxidation of arachidonic acid to hydroperoxyarachidonates. These products are then rapidly converted by a peroxidase to hydroxyeicosatetraenoic acids. The positional specificity of the enzyme reaction varies from tissue to tissue. The final lipoxygenase pathway leads to the leukotrienes. EC 1.13.11.- .
An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.
A lipoxygenase metabolite of ARACHIDONIC ACID. It is a highly selective ligand used to label mu-opioid receptors in both membranes and tissue sections. The 12-S-HETE analog has been reported to augment tumor cell metastatic potential through activation of protein kinase C. (J Pharmacol Exp Ther 1995; 274(3):1545-51; J Natl Cancer Inst 1994; 86(15):1145-51)
A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.
An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.
(2S-(2 alpha,3 beta(1E,3E,5Z,8Z)))-3-(1,3,5,8-Tetradecatetraenyl)oxiranebutanoic acid. An unstable allylic epoxide, formed from the immediate precursor 5-HPETE via the stereospecific removal of a proton at C-10 and dehydration. Its biological actions are determined primarily by its metabolites, i.e., LEUKOTRIENE B4 and cysteinyl-leukotrienes. Alternatively, leukotriene A4 is converted into LEUKOTRIENE C4 by glutathione-S-transferase or into 5,6-di-HETE by the epoxide-hydrolase. (From Dictionary of Prostaglandins and Related Compounds, 1990)
A group of LEUKOTRIENES; (LTC4; LTD4; and LTE4) that is the major mediator of BRONCHOCONSTRICTION; HYPERSENSITIVITY; and other allergic reactions. Earlier studies described a "slow-reacting substance of ANAPHYLAXIS" released from lung by cobra venom or after anaphylactic shock. The relationship between SRS-A leukotrienes was established by UV which showed the presence of the conjugated triene. (From Merck Index, 11th ed)
(11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.
Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Chromatography, High Pressure Liquid
Fatty Acids, Unsaturated
Physiologically active compounds found in many organs of the body. They are formed in vivo from the prostaglandin endoperoxides and cause platelet aggregation, contraction of arteries, and other biological effects. Thromboxanes are important mediators of the actions of polyunsaturated fatty acids transformed by cyclooxygenase.
Platelet Activating Factor
Fatty Acids, Essential
The conjugation product of LEUKOTRIENE A4 and glutathione. It is the major arachidonic acid metabolite in macrophages and human mast cells as well as in antigen-sensitized lung tissue. It stimulates mucus secretion in the lung, and produces contractions of nonvascular and some VASCULAR SMOOTH MUSCLE. (From Dictionary of Prostaglandins and Related Compounds, 1990)
Group IV Phospholipases A2
A nonapeptide messenger that is enzymatically produced from KALLIDIN in the blood where it is a potent but short-lived agent of arteriolar dilation and increased capillary permeability. Bradykinin is also released from MAST CELLS during asthma attacks, from gut walls as a gastrointestinal vasodilator, from damaged tissues as a pain signal, and may be a neurotransmitter.
Gas Chromatography-Mass Spectrometry
Chemical agents that increase the permeability of biological or artificial lipid membranes to specific ions. Most ionophores are relatively small organic molecules that act as mobile carriers within membranes or coalesce to form ion permeable channels across membranes. Many are antibiotics, and many act as uncoupling agents by short-circuiting the proton gradient across mitochondrial membranes.
The process of converting an acid into an alkyl or aryl derivative. Most frequently the process consists of the reaction of an acid with an alcohol in the presence of a trace of mineral acid as catalyst or the reaction of an acyl chloride with an alcohol. Esterification can also be accomplished by enzymatic processes.
6-Ketoprostaglandin F1 alpha
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Prostaglandin Endoperoxides, Synthetic
The prototypical analgesic used in the treatment of mild to moderate pain. It has anti-inflammatory and antipyretic properties and acts as an inhibitor of cyclooxygenase which results in the inhibition of the biosynthesis of prostaglandins. Aspirin also inhibits platelet aggregation and is used in the prevention of arterial and venous thrombosis. (From Martindale, The Extra Pharmacopoeia, 30th ed, p5)
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Rats, Inbred Strains
A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Cytochrome P-450 Enzyme System
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Important polyunsaturated fatty acid found in fish oils. It serves as the precursor for the prostaglandin-3 and thromboxane-3 families. A diet rich in eicosapentaenoic acid lowers serum lipid concentration, reduces incidence of cardiovascular disorders, prevents platelet aggregation, and inhibits arachidonic acid conversion into the thromboxane-2 and prostaglandin-2 families.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Enzymes of the isomerase class that catalyze the oxidation of one part of a molecule with a corresponding reduction of another part of the same molecule. They include enzymes converting aldoses to ketoses (ALDOSE-KETOSE ISOMERASES), enzymes shifting a carbon-carbon double bond (CARBON-CARBON DOUBLE BOND ISOMERASES), and enzymes transposing S-S bonds (SULFUR-SULFUR BOND ISOMERASES). (From Enzyme Nomenclature, 1992) EC 5.3.
Chromatography, Thin Layer
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Group VI Phospholipases A2
Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.
Dose-Response Relationship, Drug
Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides.
Anti-Inflammatory Agents, Non-Steroidal
Anti-inflammatory agents that are non-steroidal in nature. In addition to anti-inflammatory actions, they have analgesic, antipyretic, and platelet-inhibitory actions.They act by blocking the synthesis of prostaglandins by inhibiting cyclooxygenase, which converts arachidonic acid to cyclic endoperoxides, precursors of prostaglandins. Inhibition of prostaglandin synthesis accounts for their analgesic, antipyretic, and platelet-inhibitory actions; other mechanisms may contribute to their anti-inflammatory effects.
Tumor Cells, Cultured
Receptors, Leukotriene B4
A class of cell surface leukotriene receptors with a preference for leukotriene B4. Leukotriene B4 receptor activation influences chemotaxis, chemokinesis, adherence, enzyme release, oxidative bursts, and degranulation in polymorphonuclear leukocytes. There are at least two subtypes of these receptors. Some actions are mediated through the inositol phosphate and diacylglycerol second messenger systems.
Group II Phospholipases A2
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
Group X Phospholipases A2
A secreted phospholipase A2 subtype that contains a interfacial-binding region with specificity for PHOSPHATIDYLCHOLINE. This enzyme group may play a role in eliciting ARACHIDONIC ACID release from intact cellular membranes and from LOW DENSITY LIPOPROTEINS. Members of this group bind specifically to PHOSPHOLIPASE A2 RECEPTORS.
Gene Expression Regulation, Enzymologic
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Protein Kinase C
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
Amino Acid Sequence
(9 alpha,11 alpha,13E,15S)-9,11,15-Trihydroxyprost-13-en-1-oic acid (PGF(1 alpha)); (5Z,9 alpha,11,alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic acid (PGF(2 alpha)); (5Z,9 alpha,11 alpha,13E,15S,17Z)-9,11,15-trihydroxyprosta-5,13,17-trien-1-oic acid (PGF(3 alpha)). A family of prostaglandins that includes three of the six naturally occurring prostaglandins. All naturally occurring PGF have an alpha configuration at the 9-carbon position. They stimulate uterine and bronchial smooth muscle and are often used as oxytocics.
A class of lipoproteins of small size (18-25 nm) and light (1.019-1.063 g/ml) particles with a core composed mainly of CHOLESTEROL ESTERS and smaller amounts of TRIGLYCERIDES. The surface monolayer consists mostly of PHOSPHOLIPIDS, a single copy of APOLIPOPROTEIN B-100, and free cholesterol molecules. The main LDL function is to transport cholesterol and cholesterol esters to extrahepatic tissues.
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Phospholipids which have an alcohol moiety in ethereal linkage with a saturated or unsaturated aliphatic alcohol. They are usually derivatives of phosphoglycerols or phosphatidates. The other two alcohol groups of the glycerol backbone are usually in ester linkage. These compounds are widely distributed in animal tissues.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.
A dideoxynucleoside compound in which the 3'-hydroxy group on the sugar moiety has been replaced by a hydrogen. This modification prevents the formation of phosphodiester linkages which are needed for the completion of nucleic acid chains. The compound is an inhibitor of HIV replication, acting as a chain-terminator of viral DNA by binding to reverse transcriptase. Its principal side effect is nephrotoxicity. In vivo, dideoxyadenosine is rapidly metabolized to DIDANOSINE (ddI) by enzymatic deamination; ddI is then converted to dideoxyinosine monophosphate and ultimately to dideoxyadenosine triphosphate, the putative active metabolite.
5-Lipoxygenase-Activating Protein Inhibitors
One of the biologically active principles of SRS-A. It is generated from LEUKOTRIENE C4 after partial hydrolysis of the peptide chain, i.e., cleavage of the gamma-glutamyl portion. Its biological actions include stimulation of vascular and nonvascular smooth muscle, and increases in vascular permeability. (From Dictionary of Prostaglandins and Related Compounds, 1990)
Derivatives of phosphatidic acid in which the hydrophobic regions are composed of two fatty acids and a polar alcohol is joined to the C-3 position of glycerol through a phosphodiester bond. They are named according to their polar head groups, such as phosphatidylcholine and phosphatidylethanolamine.
A flavoprotein enzyme that catalyzes the univalent reduction of OXYGEN using NADPH as an electron donor to create SUPEROXIDE ANION. The enzyme is dependent on a variety of CYTOCHROMES. Defects in the production of superoxide ions by enzymes such as NADPH oxidase result in GRANULOMATOUS DISEASE, CHRONIC.
Nonheme Iron Proteins
7-Hydroxycoumarins. Substances present in many plants, especially umbelliferae. Umbelliferones are used in sunscreen preparations and may be mutagenic. Their derivatives are used in liver therapy, as reagents, plant growth factors, sunscreens, insecticides, parasiticides, choleretics, spasmolytics, etc.
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
cDNA cloning of 15-lipoxygenase type 2 and 12-lipoxygenases of bovine corneal epithelium. (1/405)Bovine corneal epithelium contains arachidonate 12- and 15-lipoxygenase activity, while human corneal epithelium contains only 15-lipoxygenase activity. Our purpose was to identify the corneal 12- and 15-lipoxygenase isozymes. We used cDNA cloning to isolate the amino acid coding nucleotide sequences of two bovine lipoxygenases. The translated sequence of one lipoxygenase was 82% identical with human 15-lipoxygenase type 2 and 75% identical with mouse 8-lipoxygenase, whereas the other translated nucleotide sequence was 87% identical with human 12-lipoxygenase of the platelet type. Expression of 15-lipoxygenase type 2 and platelet type 12-lipoxygenase mRNAs were detected by Northern analysis. In addition to these two lipoxygenases, 12-lipoxygenase of leukocyte (tracheal) type was detected by polymerase chain reaction (PCR), sequencing, and Northern analysis. Finally, PCR and sequencing suggested that human corneal epithelium contains 15-lipoxygenase types 1 and 2. (+info)
Oxidation of low density lipoprotein and plasma by 15-lipoxygenase and free radicals. (2/405)It is generally accepted that the oxidation of pentadiene structures of polyunsaturated lipids by lipoxygenase (LOX) is regio- and enantio-specific, while the free radical-mediated lipid peroxidation gives stereo-random racemic products. It was confirmed that the oxidation of human low density lipoprotein (LDL) by 15-LOX from rabbit reticulocytes gave phosphatidylcholine (PC) and cholesteryl ester (CE) hydroperoxides regio-, stereo- and enantio-specifically. 15-LOX also oxidized human plasma to give specific PC and CE hydroperoxides in spite of the presence of high concentrations of antioxidants. More CE hydroperoxides were formed than PC hydroperoxides from LDL, but the reverse order was observed for plasma oxidation. The S/R ratio of the hydroperoxides decreased during long time incubation but remained significantly larger than one, while free radical-mediated oxidation of LDL and plasma gave racemic products. (+info)
Tissue-specific translational regulation of alternative rabbit 15-lipoxygenase mRNAs differing in their 3'-untranslated regions. (3/405)By screening a rabbit reticulocyte library, an alternative 15-LOX transcript of 3.6 kb (15-LOX mRNA2) was detected containing a 1019 nt longer 3'-untranslated region (UTR2) than the main 2.6 kb mRNA (15-LOX mRNA1). In anaemic animals, northern blotting showed that 15-LOX mRNA2 was predominantly expressed in non-erythroid tissues, whereas 15-LOX mRNA1 was exclusively expressed in red blood cells and bone marrow. The 15-LOX 3'-UTR2 mRNA2 contained a novel 8-fold repetitive CU-rich motif, 23 nt in length (DICE2). This motif is related but not identical to the 10-fold repetitive differentiation control element (DICE1) of 19 nt residing in the 15-LOX UTR1 mRNA1. DICE1 was shown to interact with human hnRNP proteins E1 and K, thereby inhibiting translation. From tissues expressing the long 15-LOX mRNA2, two to three unidentified polypeptides with molecular weights of 53-55 and 90-93 kDa which bound to DICE2 were isolated by RNA affinity chromatography. A 93 kDa protein from lung cytosol, which was selected by DICE2 binding, was able to suppress translational inhibition of 15-LOX mRNA2, but not of 15-LOX mRNA1, by hnRNP E1. A possible interaction between DICE1/DICE2 cis / trans factors in translational control of 15-LOX synthesis is discussed. Furthermore, the 3'-terminal part of the highly related rabbit leukocyte-type 12-LOX gene was analysed. Very similar repetitive CU-rich elements of the type DICE1 (20 repeats) and DICE2 (nine repeats) were found in the part corresponding to the 3'-UTR of the mRNA. (+info)
Regulation of 15-lipoxygenase expression and mucus secretion by IL-4 in human bronchial epithelial cells. (4/405)Our laboratory has recently shown that mucus differentiation of cultured normal human tracheobronchial epithelial (NHTBE) cells is accompanied by the increased expression of 15-lipoxygenase (15-LO). We used differentiated NHTBE cells to investigate the regulation of 15-LO expression and mucus secretion by inflammatory cytokines. Interleukin (IL)-4 and IL-13 dramatically enhanced the expression of 15-LO, whereas tumor necrosis factor-alpha, IL-1beta, and interferon (IFN)-gamma had no effect. These cytokines did not increase the expression of cyclooxygenase-2, with the exception of a modest induction by IL-1beta. The IL-4-induced 15-LO expression was concentration dependent, and mRNA and protein expression increased within 3 and 6 h, respectively, after IL-4 treatment. In metabolism studies with intact cells, 15-hydroxyeicosatetraenoic acid (15-HETE) and 13-hydroxyoctadecadienoic acid (13-HODE) were the major metabolites formed from exogenous arachidonic acid and linoleic acid. No prostaglandins were detected. IL-4 treatment dramatically increased the formation of 13-HODE and 15-HETE compared with that in untreated NHTBE cells, and several additional 15-LO metabolites were observed. Pretreatment of NHTBE cells with IFN-gamma or dexamethasone did not inhibit the IL-4-induced expression of 15-LO except at high concentrations (100 ng/ml of IFN-gamma and 10 microM dexamethasone). IL-4 treatment inhibited mucus secretion and attenuated the expression of the mucin genes MUC5AC and MUC5B at 12-24 h after treatment. Addition of 15-HETE precursor and 13-HODE precursor to the cultures did not alter mucin secretion or mucin gene expression. On the basis of the data presented, we conclude that the increase in 15-LO expression by IL-4 and attenuation of mucus secretion may be independent biological events. (+info)
Effects of mutant p53 expression on human 15-lipoxygenase-promoter activity and murine 12/15-lipoxygenase gene expression: evidence that 15-lipoxygenase is a mutator gene. (5/405)Human 15-lipoxygenase (h15-LO) is present on chromosome 17p13.3 in close proximity to the tumor-suppressor gene, p53. 15-LO is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. The murine BALB/c embryo fibroblast cell line, (10)1val, expresses p53 in mutant (mt) conformation when grown at 39 degrees C and in wild-type conformation when grown at 32 degrees C. Transfection of h15-LO promoter constructs (driving luciferase reporter) into (10)1val cells and into p53-deficient (10)1 cells resulted in a marked increase in h15-LO promoter activity in (10)1val cells at 39 degrees C, but not at 32 degrees C, or as compared with (10)1 cells. Transfection of h15-LO promoter deletion constructs, however, resulted in total loss of activity in both cell types at 32 degrees C and 39 degrees C. Cotransfection of (10)1 cells with h15-LO promoter (driving luciferase reporter) along with increasing levels of a mt p53 expression vector demonstrated dose-dependent capacity of mt p53 to induce 15-LO promoter activity. No effect was observed with wild-type p53. In contrast to h15-LO promoter activity, (10)1val cells had significantly lower levels of endogenous (murine) 12/15-LO (mouse analog of h15-LO) mRNA and protein when grown at 39 degrees C compared with cells grown at 32 degrees C. Our data support the hypothesis that loss of a tumor-suppressor gene (p53), or "gain-of-function activities" resulting from the expression of its mutant forms, regulates 15-LO promoter activity in man and in mouse, albeit in directionally opposite manners. The studies define a direct link between 15-LO activity and an established tumor-suppressor gene located in close chromosomal proximity. (+info)
Disruption of the 12/15-lipoxygenase gene diminishes atherosclerosis in apo E-deficient mice. (6/405)Atherosclerosis may be viewed as an inflammatory disease process that includes early oxidative modification of LDLs, leading to foam cell formation. This "oxidation hypothesis" has gained general acceptance in recent years, and evidence for the role of lipoxygenases in initiation of, or participation in, the oxidative process is accumulating. However, the relative contribution of macrophage-expressed lipoxygenases to atherogenesis in vivo remains unknown. Here, we provide in vivo evidence for the role of 12/15-lipoxygenase in atherogenesis and demonstrate diminished plasma IgG autoantibodies to oxidized LDL epitopes in 12/15-lipoxygenase knockout mice crossbred with atherosclerosis-prone apo E-deficient mice (apo E-/-/L-12LO-/-). In chow-fed 15-week-old apo E-/-/L-12LO-/- mice, the extent of lesions in whole-aorta en face preparations (198 +/- 60 microm2) was strongly reduced (P < 0.001, n = 12) when compared with 12/15-lipoxygenase-expressing controls (apo E-/-/L-12LO+/+), which showed areas of lipid deposition (15,700 +/- 2,688 microm2) in the lesser curvature of the aortic arch, branch points, and in the abdominal aorta. These results were observed despite cholesterol, triglyceride, and lipoprotein levels that were similar to those in apo E-deficient mice. Evidence for reduced lesion development was observed even at 1 year of age in apo E-/-/L-12LO-/- mice. The combined data indicate a role for 12/15-lipoxygenase in the pathogenesis of atherosclerosis and suggest that inhibition of this enzyme may decrease disease progression. (+info)
All ApoB-containing lipoproteins induce monocyte chemotaxis and adhesion when minimally modified. Modulation of lipoprotein bioactivity by platelet-activating factor acetylhydrolase. (7/405)Mildly oxidized LDL has many proinflammatory properties, including the stimulation of monocyte chemotaxis and adhesion, that are important in the development of atherosclerosis. Although ApoB-containing lipoproteins other than LDL may enter the artery wall and undergo oxidation, very little is known regarding their proinflammatory potential. LDL, IDL, VLDL, postprandial remnant particles, and chylomicrons were mildly oxidized by fibroblasts overexpressing 15-lipoxygenase (15-LO) and tested for their ability to stimulate monocyte chemotaxis and adhesion to endothelial cells. When conditioned on 15-LO cells, LDL, IDL, but not VLDL increased monocyte chemotaxis and adhesion approximately 4-fold. Chylomicrons and postprandial remnant particles were also bioactive. Although chylomicrons had a high 18:1/18:2 ratio, similar to that of VLDL, and should presumably be less susceptible to oxidation, they contained (in contrast to VLDL) essentially no platelet-activating factor acetylhydrolase (PAF-AH) activity. Because PAF-AH activity of lipoproteins may be reduced in vivo by oxidation or glycation, LDL, IDL, and VLDL were treated in vitro to reduce PAF-AH activity and then conditioned on 15-lipoxygenase cells. All 3 PAF-AH-depleted lipoproteins, including VLDL, exhibited increased stimulation of monocyte chemotaxis and adhesion. In a similar manner, lipoproteins from Japanese subjects with a deficiency of plasma PAF-AH activity were also markedly more bioactive, and stimulated monocyte adhesion nearly 2-fold compared with lipoproteins from Japanese control subjects with normal plasma PAF-AH. For each lipoprotein, bioactivity resided in the lipid fraction and monocyte adhesion could be blocked by PAF-receptor antagonists. These data suggest that the susceptibility of plasma lipoproteins to develop proinflammatory activity is in part related to their 18:1/18:2 ratio and PAF-AH activity, and that bioactive phospholipids similar to PAF are generated during oxidation of each lipoprotein. Moreover, LDL, IDL, postprandial remnant particles, and chylomicrons and PAF-AH-depleted VLDL all give rise to proinflammatory lipids when mildly oxidized. (+info)
The inhibition of mammalian 15-lipoxygenases by the anti-inflammatory drug ebselen: dual-type mechanism involving covalent linkage and alteration of the iron ligand sphere. (8/405)Mammalian lipoxygenases have been implicated in inflammation and atherosclerosis and, thus, lipoxygenase inhibitors may be of pharmacological interest. In cells, lipoxygenases occur in a catalytically silent ground state that requires activation to become active. We found that the seleno-organic drug ebselen [2-phenyl-1, 2-benzisoselenazol-3(2H)-one], which exhibits anti-inflammatory properties, irreversibly inhibited pure rabbit 15-lipoxygenase, with an IC50 in the nM range when preincubated with the enzyme in the absence of fatty acid substrates. Subsequent dialysis, gel filtration, or substrate addition did not restore the enzyme activity, and experiments with [14C]ebselen indicated a covalent linkage of the drug. The presence of sulfhydryl compounds in the incubation mixture prevented both enzyme labeling and inactivation, but we did not see any reactivation when sulfhydryl compounds were added afterward. X-ray absorption studies indicated that ebselen did alter the geometry of the iron ligand sphere, and the data are consistent with an iron complexation by the drug. When fatty acid substrate was present during lipoxygenase-ebselen interaction, the inhibitory potency was strongly reduced and a competitive mode of action was observed. These data suggest that ebselen inactivated the catalytically silent ground-state lipoxygenase irreversibly by covalent linkage and alteration of the iron ligand sphere. In contrast, it functions as a competitive inhibitor of the catalytically active enzyme species. The pharmacological relevance of ebselen as a potential in vivo lipoxygenase inhibitor will be discussed. (+info)
LOX-DB - LipOXygenases DataBase - Literature
13-S-Hydroxyoctadecadienoic acid (13-S-HODE), the product of 15-lipoxygenase (15-LOX) metabolism of linoleic acid, enhances cellular mitogenic responses to certain growth factors. Other observations have questioned whether 13-S-HODE has tumorigenic effects. Our study evaluated the hypothesis that 15-LOX-1 is overexpressed in colon cancers resulting in an increase in intracellular 13-S-HODE. 15-LOX-1 and 13-S-HODE were quantified using western blots, ELISA and immunohistochemistry in 18 human colon cancers with paired normal colonic mucosa. Additionally, 15-LOX-1 expression was measured by western blots in three transformed colonic cell lines and in a human umbilical vein endothelial cell line. Next, we evaluated 13-S-HODE effects on cellular proliferation, cell cycle distribution and apoptosis in a transformed colonic cell line (RKO). Cell cycle distributions were measured by flow cytometry and apoptosis was assessed by phase contrast microscopy, electron microscopy, flow cytometry and DNA ...
LOX-DB - LipOXygenases DataBase - Literature
BACKGROUND: Nonsteroidal anti-inflammatory drugs (NSAIDs) appear to act via induction of apoptosis-programmed cell death-as potential colorectal cancer chemopreventive agents. NSAIDs can alter the production of different metabolites of polyunsaturated fatty acids (linoleic and arachidonic acids) through effects on lipoxygenases (LOXs) and cyclooxygenases. 15-LOX-1 is the main enzyme for metabolizing colonic linoleic acid to 13-S-hydroxyoctadecadienoic acid (13-S-HODE), which induces apoptosis. In human colorectal cancers, the expression of this enzyme is reduced. NSAIDs can increase 15-LOX enzymatic activity in normal leukocytes, but their effects on 15-LOX in neoplastic cells have been unknown. We tested the hypothesis that NSAIDs induce apoptosis in colorectal cancer cells by increasing the protein expression and enzymatic activity of 15-LOX-1. METHODS: We assessed 15-LOX-1 protein expression and enzymatic activity, 13-S-HODE levels, and 15-LOX-1 inhibition in association with cellular growth ...
15-Lipoxygenase-1 in the Vasculature | Circulation Research
Lipoxygenases (LOs) constitute a heterogeneous family of enzymes that catalyze the stereoselective dioxygenation of polyunsaturated fatty acids to their corresponding hydroperoxy derivatives.1,2 In mammals, LOs are categorized with respect to their positional specificity of arachidonic acid oxygenation into 5-, 8-, 12-, and 15-LOs.1-3 These enzymes induce structural and metabolic changes in cells during a wide variety of physiological and pathological processes, such as differentiation, carcinogenesis, inflammation, and atherogenesis. Accumulating studies have suggested diverse and opposing roles for the various LO pathways in the pathogenesis of human diseases, particularly cancer and atherosclerosis.2-5 Consistent with the existence of multiple isoforms of LOs, a variety of intermediate and end products of arachidonic acids are found in various cell types, and they in turn activate diverse signaling cascades, resulting in diverse outcomes. Therefore, sophisticated understanding of the ...
Abstract 507: G Proteins are Required for Lipoxygenase EDHF Activity of Rabbit Arterial Smooth Muscle Cells | Hypertension
Arachidonic acid 15-lipoxygenase (15-LO) metabolites function as endothelium-derived hyperpolarizing factors in rabbit and human arteries. In rabbit arteries, LO metabolites mediate nitric-oxide and prostaglandin-independent relaxations to acetylcholine and AA. Previously, we characterized 11,12,15-trihydroxyeicosatrienoic acid (11,12,15-THETA) as a major vasoactive 15-LO metabolite in rabbit arteries. 11,12,15-THETA requires a specific structure for vascular activity. 11(R),12(S),15(S)-THETA causes concentration-related relaxation whereas 11(R),12(R),15(S)-THETA is without activity. The specific structure requirement suggests a role for a receptor. Therefore, we examined the role of G proteins in 11(R),12(S),15(S)-THETA vascular activity. Western immunoblot verified protein expression of Gαs, Gαi and a Gαo in rabbit endothelial and smooth muscle cells. 11(R),12(S),15(S)-THETA increased GTPγ35S binding to rabbit arterial membranes 280±25% while 11(R),12(S),15(S)-THETA was without effect. In ...
Mechanistic contribution of ubiquitous 15-lipoxygenase-1 expression loss in cancer cells to terminal cell differentiation...
TY - JOUR. T1 - Mechanistic contribution of ubiquitous 15-lipoxygenase-1 expression loss in cancer cells to terminal cell differentiation evasion. AU - Moussalli, Micheline J.. AU - Wu, Yuanqing. AU - Zuo, Xiangsheng. AU - Yang, Xiu L.. AU - Wistuba, Ignacio Ivan. AU - Raso, Maria G.. AU - Morris, Jeffrey S.. AU - Bowser, Jessica L.. AU - Minna, John D.. AU - Lotan, Reuben. AU - Shureiqi, Imad. PY - 2011/12. Y1 - 2011/12. N2 - Loss of terminal cell differentiation promotes tumorigenesis. 15-Lipoxygenase-1 (15-LOX-1) contributes to terminal cell differentiation in normal cells. The mechanistic significance of 15-LOX-1 expression loss in human cancers to terminal cell differentiation suppression is unknown. In a screen of 128 cancer cell lines representing more than 20 types of human cancer, we found that 15-LOX-1 mRNA expression levels were markedly lower than levels in terminally differentiated cells. Relative expression levels of 15-LOX-1 (relative to the level in terminally differentiated ...
The Effects of Leukocyte-Type 12/15-Lipoxygenase on Id3-Mediated Vascular Smooth Muscle Cell Growth | Arteriosclerosis,...
Convincing evidence exists implicating leukocyte-type 12/15-LO as a key player in the development of atherosclerosis and restenosis. KO of the 12/15-LO gene in apolipoprotein E- and LDL receptor-deficient mice, 2 models of robust atherosclerosis, resulted in a marked decrease in atherosclerosis development in vivo.5,7 Marked upregulation of 12/15-LO occurred in VSMCs after balloon carotid injury in rats, and restenosis was significantly blocked with a pharmacological 12/15-LO inhibitor as well as a specific ribozyme targeted to cleave 12/15-LO.4,27 Many events and pathways involving 12/15-LO have been proposed to contribute to atherosclerosis and restenosis. Much is understood concerning the ability of 12/15-LO within macrophages to participate in the oxidation of LDL, an early event in vascular lesion formation.2,7-9,28 However, 12/15-LO activity and products also increase in endothelial cells and VSMCs at the time of injury and in response to growth factors and cytokines. 12S-HETE, the ...
Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation - MORU Tropical...
Molecular enzymology of lipoxygenases -ORCA
Lipoxygenases (LOXs) are lipid peroxidizing enzymes, implicated in the pathogenesis of inflammatory and hyperproliferative diseases, which represent potential targets for pharmacological intervention. Although soybean LOX1 was discovered more than 60 years ago, the structural biology of these enzymes was not studied until the mid 1990s. In 1993 the first crystal structure for a plant LOX was solved and following this protein biochemistry and molecular enzymology became major fields in LOX research. This review focuses on recent developments in molecular enzymology of LOXs and summarizes our current understanding of the structural basis of LOX catalysis. Various hypotheses explaining the reaction specificity of different isoforms are critically reviewed and their pros and cons briefly discussed. Moreover, we summarize the current knowledge of LOX evolution by profiling the existence of LOX-related genomic sequences in the three kingdoms of life. Such sequences are found in eukaryotes and bacteria ...
Biblio | College of Public Health and Human Sciences | Oregon State University
T. Offer, Ho, E., Bruno, R. S., Traber, M., Kuypers, F., and Ames, B. N., A simple assay for frequency of chromosome breaks and loss (Micronuclei) by flow Cytometry of Human Reticulocytes, FASEB Journal, vol. 19, no. 3, pp. 485-7, 2005. ...
Planten un Blomen (W. Kordes S hne 2008) - R e - Rosa : R e - Katalog rostlin
Rosa, R e, Nov odr da mnohokv t r e, kter vynik nev edn barevnou kombinac a vynikaj c odolnost proti chorob m a mrazu. Velmi zaj mav hra barev od z iv erven po b lo- ervenou na rubu l stk . Doporu en hustota v sadby je 4 rostliny na 1 m²., R e
Human articular chondrocytes express 15-lipoxygenase-1 and -2: potential role in osteoarthritis | Arthritis Research & Therapy ...
15-Lipoxygenases and their metabolites have been shown to exhibit anti-inflammatory and immunomodulatory properties, but little is known regarding their expression and function in chondrocytes. The objective of this study was to evaluate the expression of 15-lipoxygenase-1 and -2 in human articular chondrocytes, and to investigate the effects of their metabolites 13(S)-hydroxy octadecadienoic and 15(S)-hydroxyeicosatetraenoic acids on IL-1β-induced matrix metalloproteinase (MMP)-1 and MMP-13 expression. The expression levels of 15-lipoxygenase-1 and -2 were analyzed by reverse transcription PCR and Western blotting in chondrocytes, and by immunohistochemistry in cartilage. Chondrocytes or cartilage explants were stimulated with IL-1β in the absence or presence of 13(S)-hydroxy octadecadienoic and 15(S)-hydroxyeicosatetraenoic acids, and the levels of MMP-1 and MMP-13 protein production and type II collagen cleavage were evaluated using immunoassays. The role of peroxisome proliferator-activated
Липоксигеназа - Википедија, слободна енциклопедија
Royo, J., Vancanneyt, G., Perez, A.G., Sanz, C., Stormann, K., Rosahl, S. and Sanchez-Serrano, J.J. (1996). „Characterization of three potato lipoxygenases with distinct enzymatic activities and different organ-specific and wound-regulated expression patterns. J. Biol. Chem. 271: 21012-21019. PMID 8702864 ...
The Best Scalp Massager | southmiamirealestateblog.com
Currently, the best scalp massager is the Heeta Shampoo Brush. Wiki researchers have been writing reviews of the latest scalp massagers since 2019.
Cyclooxygenase-catalyzed formation of 9-hydroxylinoleic acid by guinea pig alveolar macrophages under non-stimulated conditions
Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 × 106 AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT), ... read more a byproduct of the formation of thromboxane A2. 9-HODE and HHT were the most prominent products formed under the incubation conditions used. These products were formed via a Cyclooxygenase-catalyzed reaction, since their formation was inhibited by 1 μM indomethacin. Thus, under basal conditions AM possess substantial cyclooxygenase activity. The biological significance of 9-HODE and related substances is discussed. show less ...
ENHANCED HETEROLOGOUS PRODUCTION OF LIPOXYGENASES - US NEWWIN, INC.
The invention is directed to the enhanced expression and purification of lipoxygenase enzymes. These enzymes are of wide-spread industrial importance, often produced in heterologous microbial systems. Preferably, the lipoxygenase produced by the methods of the invention is a plant-derived enzyme and expressed at high-levels in a microbial system that includes a protease-deficient host and one or more chaperone expression plasmids. The invention is also directed to amino acid and nucleic acid fragments of the lipoxygenase enzyme including fragments in expression constructs encoding all or a portion of one or more lipoxygenase genes. The invention is also directed to methods of manufacturing bread and other food and also non-food products with lipoxygenase manufactured by the methods of the invention.
Lipoxygenase Arachidonate 15-Lipoxygenase Identifiers Symbol Lipoxygenase Pfam PF00305 InterPro IPR013819 SCOP 2sbl OPM family 87 OPM protein 1zq4 Available
LOX1.1 - Linoleate 9S-lipoxygenase A - Solanum lycopersicum (Tomato) - LOX1.1 gene & protein
Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
15 Lipoxygenase 1抗体[3G8]|Abcam中国|Anti-15 Lipoxygenase 1抗体[3G8]
15 Lipoxygenase 1小鼠单克隆抗体[3G8](ab119774)可与大鼠, 狗, 人, 猴样本反应并经WB, IHC, ICC/IF实验严格验证。所有产品均提供质保服务，中国75%以上现货。
Discovery of selective imidazole-based inhibitors of mammalian 15-lipoxygenase: Highly potent against human enzyme within a...
Discovery of selective imidazole-based inhibitors of mammalian 15-lipoxygenase: Highly potent against human enzyme within a cellular environment ...
Recombinant Human 5 Lipoxygenase protein (ab114310) References
References for Abcams Recombinant Human 5 Lipoxygenase protein (ab114310). Please let us know if you have used this product in your publication
Recombinant Human 15 Lipoxygenase 1 protein (ab114421) References
References for Abcams Recombinant Human 15 Lipoxygenase 1 protein (ab114421). Please let us know if you have used this product in your publication
linoleate 11-lipoxygenase(EC 184.108.40.206) - Creative Enzymes
The product (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate, is converted, more slowly, into (9Z,11E)-(13R)-13-hydroperoxyoctadeca-9,11-dienoate. The enzyme from the
Papain kinetics in the presence of a water-miscible organic solvent - Fernandez - 1991 - Biotechnology and Bioengineering -...
The effects of various concentrations of a water-miscible organic solvent [a 7:3 (v/v) mixture of N, N dimethylformamide and dimethylsulfoxide] on the kinetics of papain have been investigated. The parameters kcat and Km for the amidase and esterase activity of papain using N-α-benzoyl-DL-arginine-p-nitroanilide (BAPNA) and N-α-benzoyl-L-arginine ethyl ester (BAEE) as substrates were determined. For both types of activity, kcat initially increased (up to about 15% solvent), and then decreased with increasing concentrations of organic solvent. In contrast, Km increased sharply with the organic solvent concentration. Active site titration at 0 and 50% solvent indicated no change in the amount of active enzyme. Fluorometric measurements of the emission spectrum of papain did not indicate any major conformational changes with increasing concentrations of organic solvent. ...
Accepted name: arachidonate 12-lipoxygenase. Reaction: arachidonate + O2 = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate. Other name(s): δ12-lipoxygenase; 12-lipoxygenase; 12δ-lipoxygenase; C-12 lipoxygenase; 12S-lipoxygenase; leukotriene A4 synthase; LTA4 synthase. Systematic name: arachidonate:oxygen 12-oxidoreductase. Comments: The product is rapidly reduced to the corresponding 12S-hydroxy compound.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 82391-43-3. References:. 1. Hamberg, M. and Samuelsson, B. Prostaglandin endoperoxides. Novel transformations of arachidonic acid in human platelets. Proc. Natl. Acad. Sci. USA 71 (1974) 3400-3404. [PMID: 4215079]. 2. Nugteren, D.H. Arachidonate lipoxygenase in blood platelets. Biochim. Biophys. Acta 380 (1975) 299-307. [PMID: 804329]. 3. Wallach, D.P. and Brown, V.R. A novel preparation of human platelet lipoxygenase. Characteristics and inhibition by a variety of phenyl hydrazones and ...
Lipoxygenase - Wikipedia
Lipoxygenases (EC 1.13.11.-) are a family of (non-heme), iron-containing enzymes most of which catalyze the dioxygenation of polyunsaturated fatty acids in lipids containing a cis,cis-1,4- pentadiene into cell signaling agents that serve diverse roles as autocrine signals that regulate the function of their parent cells, paracrine signals that regulate the function of nearby cells, and endocrine signals that regulate the function of distant cells. The lipoxygenases are related to each other based upon their similar genetic structure and dioxygenation activity. However, one lipoxygenase, ALOXE3, while having a lipoxygenase genetic structure, possesses relatively little dioxygenation activity; rather its primary activity appears to be as an isomerase that catalyzes the conversion of hydroperoxy unsaturated fatty acids to their 1,5-epoxide, hydroxyl derivatives. Lipoxygenases are found in eukaryotes (plants, fungi, animals, protists); while the third domain of terrestrial life, the archaea, ...
14-3-3 Proteins and a 13-lipoxygenase form associations in a phosphorylation-dep :: TNO Repository
Recently, we have demonstrated by two different methods that lipoxgenases (LOXs) and 14-3-3 proteins form interactions in barley embryos [Holtman, Roberts, Oppedijk, Testerink, van Zeijl and Wang (2000) FEBS Lett. 474, 48-52]. It was shown by both co-immunoprecipitations and surface-plasmon resonance experiments that 13-LOX, but not 9-LOX, forms interactions with 14-3-3 proteins. In the present report we show that the presence of 13-LOX and 14-3-3 proteins was established in high-molecular-mass complexes. Amounts of 13-LOX and 14-3-3 proteins in high-molecular-mass fractions increased during germination, but were reduced after dephosphorylation of protein extracts or competition with the 14-3-3-binding peptide P-Raf-259, indicating that 13-LOX and 14-3-3 proteins interact in a phosphorylation-dependent manner. Chemicals/CAS: 13-lipoxygenase, EC 1.13.11.-; 14-3-3 Proteins; Lipoxygenase, EC 220.127.116.11; Tyrosine 3-Monooxygenase, EC 18.104.22.168 ...
anti Arachidonate 5 Lipoxygenase ALOX5 - Journal for the International Association for Laboratory Learning and Technology
Description: Quantitativesandwich ELISA kit for measuring Rat Arachidonate 5-lipoxygenase (Alox5) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits ...
Lipoxygenase-derived metabolites of arachidonic acid are not involved in the endothelium-dependent hyperpolarization to...
The present study was designed to determine whether or not lipoxygenase-dependent metabolites of arachidonic acid are involved in the endothelium-dependent
AA1777 | CAS# 90316-11-3| 5-Lipoxygenase Inhibitor | MedKoo
A1777 is a selective 5-lipoxygenase inhibitor that reduces leukocytes proliferation without affecting the eosinophils of mast cells.
Patent US20030167544 - Low lipoxygenase 1 barley - Google Patentsuche
Barley plants having reduced lipoxygenase-1 enzyme activity are provided, for example, barley plants expressing mutant LOX-1 protein. The barley plants of the invention are useful in the production of plant products such as malt and brewed beverages, particularly beer, having increased stability and reduced T2N potential.
15 Lipoxygenase 2 Antibody 13073-1-AP | Proteintech
15 Lipoxygenase 2 Polyclonal antibody Antibody 13073-1-AP has been identified with WB, ELISA. 13073-1-AP detected 67-76 kDa band in A431 cells with 1:500-1:3000 dilution...
Enzymatic properties of the 15-lipoxygenase of human cultured keratinocytes - Fingerprint - UC Davis
Fingerprint Dive into the research topics of Enzymatic properties of the 15-lipoxygenase of human cultured keratinocytes. Together they form a unique fingerprint. ...
Nontoxic low odor heat transfer fluid Dynalene LO-170 buy online store
Dynalene LO-170 heat transfer fluid is designed to exhibit thermally effective, nontoxic, low odor and high flash point qualities; normally found in more expensive alternatives.
Arahidonat 15-lipoksigenaza - Википедија, слободна енциклопедија
Bryant, R.W., Bailey, J.M., Schewqe, T. and Rapoport, S.M. (1982). „Positional specificity of a reticulocyte lipoxygenase. Conversion of arachidonic acid to 15-S-hydroperoxy-eicosatetraenoic acid. J. Biol. Chem. 257: 6050-6055. PMID 6804460 ...
Phylogeny Analysis - AP bio
Looking at my sequence of animals, there are a few conclusions I can come to. One conclusion I can come to is that my protein, lipoxygenase, must be very common because a variety of animals are in the sequence you may not think of as relatives. Sense lipoxygenase is involved in the metabolism that shows…
Family » 2.1.02.01. Lipoxygenases
Glycine max (1yge, 1f8n, 1fgm, 1fgo, 1fgq, 1fgr, 1fgt, 1y4k, 2sbl, 3bnb, 3bnc, 3bnd, 3bne, 3pzw, 4wfo, 4wha, 5eeo, 5t5v, 5tqn, 5tqo, 5tqp, 5tr0 ...
ω-Oxidation impairs oxidizability of polyenoic fatty acids by 15-lipoxygenases: consequences for substrate orientation at the...
During oxygenation by 15-lipoxygenases, polyenoic fatty acids are bound at the active site in such a way that the ω-terminus of the fatty acids penetrates into the substrate binding pocket. In contrast, for arachidonic acid 5-lipoxygenation, an inverse head to tail orientation has been suggested. However, an inverse orientation may be hindered by the large energy barrier associated with burying the charged carboxylate group in the hydrophobic environment of the substrate binding cleft. We studied the oxygenation kinetics of ω-modified fatty acids by 15-lipoxygenases and found that ω-hydroxylation strongly impaired substrate affinity (higher Km), but only moderately altered Vmax. In contrast, ω-carboxylation completely prevented the lipoxygenase reaction; however, methylation of the additional carboxylate group restored the activity. Arg403 of the human 15-lipoxygenase has been implicated in fatty acid binding by forming a salt bridge with the carboxylate group, and thus mutation of this ...
13-Hydroxyoctadecadienoic acid - Wikipedia
13-Hydroxyoctadecadienoic acid (13-HODE) is the commonly used term for 13(S)-hydroxy-9Z,11E-octadecadienoic acid (13(S)-HODE). The production of 13(S)-HODE is often accompanied by the production of its stereoisomer, 13(R)-hydroxy-9Z,11E-octadecadienoic acid (13(R)-HODE). The adjacent figure gives the structure for the (S) stereoisomer of 13-HODE. Two other naturally occurring 13-HODEs that may accompany the production of 13(S)-HODE are its cis-trans (i.e., 9E,11E) isomers viz., 13(S)-hydroxy-9E,11E-octadecadienoic acid (13(S)-EE-HODE) and 13(R)-hydroxy-9E,11E-octadecadienoic acid (13(R)-EE-HODE). Studies credit 13(S)-HODE with a range of clinically relevant bioactivities; recent studies have assigned activities to 13(R)-HODE that differ from those of 13(S)-HODE; and other studies have proposed that one or more of these HODEs mediate physiological and pathological responses, are markers of various human diseases, and/or contribute to the progression of certain diseases in humans. Since, however, ...
Isolation of a cDNA clone for pea (Pisum sativum) seed lipoxygenase | Biochemical Journal | Portland Press
A lipoxygenase cDNA clone, pCD45, was identified in a Pisum sativum L. (pea) seed mRNA cDNA library by hybrid-release/translation followed by immunoprecipitation with antiserum raised against lipoxygenase from Glycine max L. (soya bean). pCD45 hybrid-selected an mRNA encoding the larger of the two polypeptides of Mr approximately 95 000 that were immunoprecipitated from cell-free translation products of pea seed poly(A)-containing RNA by the G. max anti-lipoxygenase. Northern-blot analysis showed the mRNA that hybridized to pCD45 to be approximately 3000 nucleotides in length. Three to five copies of the lipoxygenase gene corresponding to pCD45 were estimated to be present per haploid Pisum genome; hybridization of the cDNA insert from pCD45 to G. max DNA was also detected. ...
Structural Organization of the Regulatory Domain of Human 5- Lipoxygenase | Bentham Science
Title: Structural Organization of the Regulatory Domain of Human 5- Lipoxygenase. VOLUME: 6 ISSUE: 2. Author(s):John B. Allard and Thomas G. Brock. Affiliation:6301 MSRB III, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109-0642, USA.. Keywords:5-lipoxygenase, structure, c2 domain, toxin, lipase, protein kinase c, sandwich, plat domain. Abstract: The enzyme 5-lipoxygenase (5-LO) initiates the synthesis of leukotrienes. For this reason, 5-LO activity is important for immune defense, whereas improper regulation contributes to pathogenesis, including chronic inflammation, asthma and atherosclerosis. Like all lipoxygenases, the 5-LO protein consists of two domains, a regulatory domain and a catalytic domain. Naturally, the regulatory domain determines catalytic activity and controls leukotriene synthesis. This domain shares features with classical C2 domains in that it has a β-sandwich structure and binds calcium, nucleotides and phospholipids. However, important ...
Human CYP1B1 Q16678 Information | Boster Bio
Human CYP1B1 Information And Facts | common name: CYP1B1. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta- estradiol to the carcinogenic 4-hydroxy derivative, and many different procarcinogenic chemicals to their activated forms, such as polycyclic aromatic hydrocarbons. Promotes angiogenesis by eliminating cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular
Hydroxyoctadecadienoic acids (HODEs) regulate fatty acid binding protein-4 (FABP4) secretion in human monocytes and macrophages...
9-HODE is an oxidation product of linoleic acid (LA, C18:2), is pro-inflammatory, and is a ligand for GPR132 which is involved in atherogenesis. By contrast, 13-HODE is protective and acts through PPARgamma. Plasma FABP4 is increased in diabetes and coronary artery disease. 9-HODE increases FABP4 expression in macrophages. We investigated whether GPR132 is a monocyte activation marker in diabetes, and if it mediates the effect of 9-HODE on FABP4. Monocyte populations from 31 type 2 diabetic patients and controls were studied using FACS. Plasma cytokines were measured using bead arrays and ELISA. THP-1 cells were used to investigate regulation of FABP4 secretion. Diabetic subjects had increased circulating CD14+, CD14+CD36+, CD14+CD11b+, CD14+CD54+ cells (p,0.01), and also increased GPR132 mRNA expression in CD14+ monocytes (p , 0.01). Levels of GPR132 expression did not correlate with any of the above cell populations, or with increased plasma levels of FABP4, sTNF-R, osteoprotegerin, MCP-1, ...
Arachidonate 15-lipoxygenase type B knockdown leads to reduced lipid accumulation and inflammation in atherosclerosis.
Inflammation in the vascular wall is important for development of atherosclerosis. We have shown previously that arachidonate 15-lipoxygenase type B (ALOX15B) is more highly expressed in human atherosclerotic lesions than in healthy arteries. This en
Researcher - ISGLOBAL
Reticulocyte-derived exosomes (Rex) vaccines against P. vivax. We previously found that exosomes derived from reticulocytes infected with malaria parasites can induce a long-lasting protective immune response. More recently, we determined the proteome of plasma-derived exosomes from P. vivax patients and of human reticulocyte exosomes and showed that they contain MHC class I molecules that are actively uptaken by human dendritic cells. We are pursuing efforts to tailor human Rex with P. vivax antigens and determine their potential as a new delivery platform for P. vivax vaccines. (Martin-Jaular et al., Plos One 2011, Diaz-Varela et al., Sci. Rep 2018 ...
Miembros del equipo - ISGLOBAL
Reticulocyte-derived exosomes (Rex) vaccines against P. vivax. We previously found that exosomes derived from reticulocytes infected with malaria parasites can induce a long-lasting protective immune response. More recently, we determined the proteome of plasma-derived exosomes from P. vivax patients and of human reticulocyte exosomes and showed that they contain MHC class I molecules that are actively uptaken by human dendritic cells. We are pursuing efforts to tailor human Rex with P. vivax antigens and determine their potential as a new delivery platform for P. vivax vaccines. (Martin-Jaular et al., Plos One 2011, Diaz-Varela et al., Sci. Rep 2018 ...
Docking studies: In silico lipoxygenase inhibitory activity of some commercially available flavonoids - ABIM - An Annotated...
Madeswaran, Arumugam, Muthuswamy Umamaheswari, Kuppuswamy Asokkumar, Thirumalaisamy Sivashanmugam, Varadharajan Subhadradevi, Puliyath Jagannath (2011) Docking studies: In silico lipoxygenase inhibitory activity of some commercially available flavonoids. [Publication] Full text not available from this repository ...
Inhibition of 15-lipoxygenases by flavonoids: structure-activity relations and mode of action. | Harvard Catalyst Profiles |...
Inhibition of 15-lipoxygenases by flavonoids: structure-activity relations and mode of action. Biochem Pharmacol. 2003 Mar 01; 65(5):773-81 ...
ALOX15 Gene - GeneCards | LOX15 Protein | LOX15 Antibody
Complete information for ALOX15 gene (Protein Coding), Arachidonate 15-Lipoxygenase, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Superfamily » 2.1.002. Lipoxygenase (1 family)
1yge, 1f8n, 1fgm, 1fgo, 1fgq, 1fgr, 1fgt, 1y4k, 2sbl, 3bnb, 3bnc, 3bnd, 3bne, 3pzw, 4wfo, 4wha, 5eeo, 5t5v, 5tqn, 5tqo, 5tqp, 5tr0 ...
5-Lipoxygenase | ChemScene llc
Contact Us. Tel:732-484-9848. Fax:888-484-5008. Email:[email protected]. Add:1 Deer Park Dr, Suite Q,. Monmouth Junction, NJ 08852, USA. ...
5-lipoxygenase, 12-lipoxygenase, and 15-lipoxygenase-2, and selected metabolites of the latter lipoxygenases show no such ... Kuhn, Hartmut; Walther, Matthias; Kuban, Ralf Jürgen (2002). "Mammalian arachidonate 15-lipoxygenases". Prostaglandins & Other ... "Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid ... Folcik, V A; Nivar-Aristy, R A; Krajewski, L P; Cathcart, M K (1995). "Lipoxygenase contributes to the oxidation of lipids in ...
... , also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13 ... Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. It transforms essential fatty acids (EFA) ... "Alox5 - arachidonate 5-lipoxygenase". WikiGenes. Fahel JS, de Souza MB, Gomes MT, Corsetti PP, Carvalho NB, Marinho FA, de ... Arachidonate+5-Lipoxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Human ALOX5 genome location ...
Arachidonate 5-lipoxygenase inhibitor
... s are compounds that slow or stop the action of the arachidonate 5-lipoxygenase (5- ... 1. Arachidonate 5-lipoxygenase ...Specific function: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a ... Antileukotriene agents Arachidonate 5-lipoxygenase ALOX5-inhibiting drugs David L. Nelson, Michael M. Cox. Lehninger's ... Lipoxygenase+inhibitors at the US National Library of Medicine Medical Subject Headings (MeSH) MeSH list of agents 82016859 v t ...
Arachidonate 15-lipoxygenase type II is an enzyme that in humans is encoded by the ALOX15B gene. ALOX15B, also known as 15- ... Xu XC, Shappell SB, Liang Z, Song S, Menter D, Subbarayan V, Iyengar S, Tang DG, Lippman SM (2003). "Reduced 15S-lipoxygenase-2 ... "Entrez Gene: ALOX15B arachidonate 15-lipoxygenase, type B". Human ALOX15B genome location and ALOX15B gene details page in the ... Brash AR, Boeglin WE, Chang MS (Jun 1997). "Discovery of a second 15S-lipoxygenase in humans". Proceedings of the National ...
... an arachidonate lipoxygenase product". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1136 (3): 247-52. doi: ... inhibitor of 12-lipoxygenase but not other human lipoxygenases. This effect could also have anti-inflammatory and anti- ... Oxygenated by 5-lipoxygenase (ALOX5 to form its 5,6-trans epoxide derivative which may then rearrange to the lipoxins (LX), ... Brash, A. R.; Boeglin, W. E.; Chang, M. S. (1997). "Discovery of a second 15S-lipoxygenase in humans". Proceedings of the ...
ALOX12B (i.e. arachidonate 12-lipoxygenase, 12R type) forms R chirality products, i.e. 12R-HpETE and 12R-HETE. Similarly, ... They include (HEPE is hydroxy-eicsapentaenoic acid): 5-HEPE (see Arachidonate 5-lipoxygenase#Eicosapentaenoic acid), 12-HEPE, ... Lipoxygenases (LOXs): 5-Lipoxygenase (5-LOX or ALOX5) initiates the metabolism of arachidonic acid to 5- ... see Arachidonate 5-lipoxygenase#Eicosapentaenoic acid); PGE3, PGD3, PGF3α, and Δ(17)-6-keto PGF1α; PGI3 (see Essential fatty ...
... leukocyte-type arachidonate 12-lipoxygenase, or arachidonate omega-6 lipoxygenase. The second discovered human 15-lipoxygenase ... omega-6 lipoxygenase) from human leukocytes. Purification and structural homology to other mammalian lipoxygenases". The ... whereas the most unrelated lipoxygenase (5-lipoxygenase) was mapped to chromosome 10. Kelavkar and Badr (1999) stated that the ... ALOX15 (also termed arachidonate 15-lipoxygenase, 15-lipoxygenase-1, 15-LO-1, 15-LOX-1) is, like other lipoxygenases, a seminal ...
... oxygenation of this arachidonic acid by activated arachidonate 5-lipoxygenase (ALOX5) to form 5(S)-hydroperoxy-6E,8Z,11Z,14Z- ... Second, human platelets use 12-lipoxygenase to metabolize 5-oxo-ETE to 5-oxo-12(S)-hydroperxy-eicosatetraenoat which is rapidly ... This transcellular production typically involves the limited variety of cell types that express active 5-lipoxygenase, lack ... First, human eosinophils use Arachidonate 15-lipoxygenase-1 (or possibly Arachidonate 15-lipoxygenase-2 to metabolize 5-oxo-ETE ...
List of MeSH codes (D12.776)
... arachidonate 5-lipoxygenase MeSH D12.776.556.579.374.450.025.025 - arachidonate 12-lipoxygenase MeSH D12.776.556.579.374.450. ... lipoxygenase MeSH D12.776.556.579.374.450.025 - arachidonate lipoxygenases MeSH D12.776.556.579.374.450.025.020 - ... arachidonate 15-lipoxygenase MeSH D12.776.556.579.374.687 - retinal dehydrogenase MeSH D12.776.556.579.374.925 - tyrosine 3- ... interleukin-15 MeSH D12.776.467.374.400.505.516 - interleukin-16 MeSH D12.776.467.374.400.505.517 - interleukin-17 MeSH D12.776 ...
List of MeSH codes (D08)
... arachidonate 5-lipoxygenase MeSH D08.811.682.690.416.444.050.060 - arachidonate 12-lipoxygenase MeSH D08.811.682.690.416.444. ... lipoxygenase MeSH D08.811.682.690.416.444.050 - arachidonate lipoxygenases MeSH D08.811.682.690.416.444.050.055 - ... 050.065 - arachidonate 15-lipoxygenase MeSH D08.811.682.690.416.444.525 - protocatechuate 3,4-dioxygenase MeSH D08.811.682.690. ... beta-carotene 15,15'-monooxygenase MeSH D08.811.682.690.708.392.468 - Linoleoyl-CoA desaturase MeSH D08.811.682.690.708.392.625 ...
In humans, Arachidonate 12-lipoxygenase (12-LO, 12-LOX, ALO12, or platelet type 12-lipoxygenase) is encoded by the ALOX12 gene ... 12-lipoxygenase gene, see lipoxygenase#mouse lipoxygenases) are resistant to a) streptozotocin-induced, b) high fat diet- ... Arachidonate 12-lipoxygenase, 12R type, also termed 12RLOX and encoded by the ALOX12B gene, is expressed primarily in skin and ... Nugteren, D. H. (1975). "Arachidonate lipoxygenase in blood platelets". Biochimica et Biophysica Acta (BBA) - Lipids and Lipid ...
... also termed arachidonate-5-lipoxygenase, 5-lipoxygenase, 5-LO, and 5-LOX). ALOX5 metabolizes arachidonic acid to its ... 12-Lipoxygenase (i.e. ALOX12) to metabolize 5(S)-HETE to 5(S),12(S)-diHETE. The activity of this product has not yet been fully ... by the arachidonate 15-Lipoxygenase-1-based or arachidonate 15-lipoxygenased-2-based metabolism of 5-oxo-ETE; and f) conversion ... The relative potencies of 5-oxo-ETE, 5-oxo-15(S)-HETE, 5-(S)-HETE, 5-(S),15-(S)-diHETE, 5-oxo-20-hydroxy-ETE, 5-(S),20-diHETE, ...
Arachidonate 5-lipoxygenase-activating protein also known as 5-lipoxygenase activating protein, or FLAP, is a protein that in ... Zintzaras E, Rodopoulou P, Sakellaridis N (March 2009). "Variants of the arachidonate 5-lipoxygenase-activating protein ( ... "Associations of genetic polymorphisms of arachidonate 5-lipoxygenase-activating protein with risk of coronary artery disease in ... "Cytogenetic and radiation hybrid mapping of human arachidonate 5-lipoxygenase-activating protein (ALOX5AP) to chromosome 13q12 ...
"ALOX5 arachidonate 5-lipoxygenase [Homo sapiens (human)] - Gene - NCBI". Haeggström, J. Z.; Funk, C. D. (2011). "Lipoxygenase ... Arachidonate 12-lipoxygenase (ALOX12) (EC 22.214.171.124InterPro: IPR001885), also termed 12-lipoxygenase, platelet type platelet ... LOX-DB - LipOXygenases DataBase Lipoxygenases iron-binding region in PROSITE PDB: 1YGE - structure of lipoxygenase-1 from ... There are several lipoxygenase structures known including: soybean lipoxygenase L1 and L3, coral 8-lipoxygenase, human 5- ...
Arachidonate 5-lipoxygenase Arachidonate 15-lipoxygenase Leukotriene 15-Hydroxyicosatetraenoic acid Greene ER, Huang S, Serhan ... The primary product of the lipoxygenase, 15-HPETE is believed to react with the enzyme further to produce the 14,15-epoxide, ... lipoxygenases (5-LOX, 12-LOX, 15-LOXa, 15-LOXb), and cytochrome P450s, respectively. These pathways are the target of approved ... is then converted to a series of eoxins by the same enzymes that metabolize the 5-lipoxygenase product of arachidonic acid ...
... also known as arachidonate 12-lipoxygenase, 12-lipoxygenase, 12S-Lipoxygenase, 12-LOX, and 12S-LOX is a lipoxygenase-type ... "Entrez Gene: ALOX12 arachidonate 12-lipoxygenase". Yamamoto S, Suzuki H, Ueda N (March 1997). "Arachidonate 12-lipoxygenases". ... arachidonate:oxygen 12-oxidoreductase, Delta12-lipoxygenase, 12Delta-lipoxygenase, and C-12 lipoxygenase. ALOX12, often termed ... Nugteren DH (February 1975). "Arachidonate lipoxygenase in blood platelets". Biochimica et Biophysica Acta (BBA) - Lipids and ...
List of EC numbers (EC 1)
... arachidonate 12-lipoxygenase EC 126.96.36.199: Now EC 188.8.131.52, nitronate monooxygenase EC 184.108.40.206: arachidonate 15- ... lipoxygenase EC 220.127.116.11: arachidonate 5-lipoxygenase EC 18.104.22.168: pyrogallol 1,2-oxygenase EC 22.214.171.124: chloridazon- ... inoleate 8R-lipoxygenase EC 126.96.36.199: linolenate 9R-lipoxygenase EC 188.8.131.52: linoleate 10R-lipoxygenase EC 184.108.40.206: β- ... arachidonate 8-lipoxygenase EC 220.127.116.11: 2,4′-dihydroxyacetophenone dioxygenase EC 18.104.22.168: identical to EC 22.214.171.124, ...
Oxoeicosanoid receptor 1
Ghosh J, Myers CE (1998). "Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells ... mediates the survival-promoting effects of arachidonate 5-lipoxygenase in prostate cancer cells". Cancer Lett. 336 (1): 185-95 ... Hussey HJ, Tisdale MJ (1996). "Inhibition of tumour growth by lipoxygenase inhibitors". Br. J. Cancer. 74 (5): 683-687. doi: ... Wijkander J, O'Flaherty JT, Nixon AB, Wykle RL (1995). "5-Lipoxygenase products modulate the activity of the 85-kDa ...
... s are synthesized in the cell from arachidonic acid by arachidonate 5-lipoxygenase. The catalytic mechanism involves ... and donated by the 5-lipoxygenase-activating protein (FLAP) to 5-lipoxygenase. 5-Lipoxygenase (5-LO) uses FLAP ... by the enzyme arachidonate 5-lipoxygenase. Leukotrienes use lipid signaling to convey information to either the cell producing ... Recent research points to a role of 5-lipoxygenase in cardiovascular and neuropsychiatric illnesses. Leukotrienes are very ...
Arachidonate 5-lipoxygenase, B-cell linker, BCAR1, BCR gene, Beta-2 adrenergic receptor, C-Met, CBLB, CD117, CD22, CD28, CDKN1B ... Lepley RA, Fitzpatrick FA (September 1994). "5-Lipoxygenase contains a functional Src homology 3-binding motif that interacts ... "Competitive binding assay of src homology domain 3 interactions between 5-lipoxygenase and growth factor receptor binding ... 15 (13): 1565-72. doi:10.1038/sj.onc.1201518. PMID 9380408. Sahni M, Zhou XM, Bakiri L, Schlessinger J, Baron R, Levy JB ( ...
When acting on GLA, arachidonate 5-lipoxygenase produces no leukotrienes and the conversion by the enzyme of arachidonic acid ... Borage oil contains 20% GLA, evening primrose oil ranges from 8% to 10% GLA, and black-currant oil contains 15-20%. The human ... DGLA itself cannot be converted to LTs but can form a 15-hydroxyl derivative that blocks the transformation of arachidonic acid ...
Arachidonate 5-Lipoxygenase (processes essential fatty acids to leukotrienes, which are important agents in the inflammatory ... protocadherin 15 PI4K2A: phosphatidylinositol 4-kinase 2-alpha PIP4K2A: phosphatidylinositol 5 phosphate 4-kinase type-2 alpha ... encoding protein F-box and leucine rich repeat protein 15 FGFR2: fibroblast growth factor receptor 2 (bacteria-expressed kinase ...
"Entrez Gene: ALOXE3 arachidonate lipoxygenase 3". Schneider C, Brash AR (August 2002). "Lipoxygenase-catalyzed formation of R- ... Epidermis-type lipoxygenase 3 (ALOXE3 or eLOX3) is a member of the lipoxygenase family of enzymes; in humans, it is encoded by ... The epidermis-type lipoxygenases are now regarded as a distinct subclass within the multigene family of mammalian lipoxygenases ... "Lipoxygenase-3 (ALOXE3) and 12(R)-lipoxygenase (ALOX12B) are mutated in non-bullous congenital ichthyosiform erythroderma (NCIE ...
... is an enzyme that metabolizes an eicosanoid product of arachidonate 5-lipoxygenase (5-LOX), 5(S)-hydroxy-6S,8Z,11Z,14Z- ... A 15-hydroxyicosatetraenoate dehydrogenase metabolizes 15-Hydroxyicosatetraenoic acid (i.e. 15(S)-hydroxy-5Z,8Z,11Z,13E- ... 15(S)-HETE, leukotriene B4, a racemate mixture of 9-HETE, a racemate mixture of 11-HETE, or a 5(S)-hydroxy-6-trans 12 carbon ... to its 15-keto analog, 15-oxo-ETE (see 15-Hydroxyicosatetraenoic acid using NAD+ and NADH rather than NADP+ and NADPH as its co ...
Arachidonate 5-lipoxygenase has been identified as playing a significant role in the survival of prostate cancer cells. ... In particular, arachidonate 5-lipoxygenase inhibitors produce massive, rapid programmed cell death in prostate cancer cells. ... Ghosh J, Myers CE (October 1998). "Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate ... The 5-lipoxygenase (5-LOX) pathway is implicated in the development and progression of human cancers. 5-LOX, whose crystal ...
Cysteinyl-leukotriene type 1 receptor antagonists
Antileukotriene Arachidonate 5-lipoxygenase inhibitor Antihistamines Hooper, Nigel M. (2013). Membrane dipeptidase. Handbook of ... Leukotrienes are produced from arachidonic acid by 5-lipoxygenase (which is made from phospholipids in the cell membrane) and ... 58 (15): 6093-6113. doi:10.1021/acs.jmedchem.5b00741. ISSN 0022-2623. PMID 26200813. (Articles with short description, Short ...
... see Lipoxygenase#Biological function and classification#Human lipoxygenases) that also contains a very long chain fatty acid. ... and concurrently the 8Z double bond in this arachidonate moves to the 9E position to form the intermediate product, 12S- ... Muñoz-Garcia, A; Thomas, C. P.; Keeney, D. S.; Zheng, Y; Brash, A. R. (2014). "The importance of the lipoxygenase-hepoxilin ... Muñoz-Garcia, A; Thomas, C. P.; Keeney, D. S.; Zheng, Y; Brash, A. R. (2014). "The importance of the lipoxygenase-hepoxilin ...
... arachidonate 5-lipoxygenase) or leukotriene receptor antagonists (cysteinyl leukotriene receptors), and consequently oppose the ... 15 (11): 527-532. doi:10.1016/0167-5699(94)90209-7. PMID 7802923. Mathison, Ronald D.; Malkinson, Terrance; Cooper, K.E.; ... Ottani, Alessandra; Leone, Sheila; Sandrini, Maurizio; Ferrari, Anna; Bertolini, Alfio (February 15, 2006). "The analgesic ...
Essential fatty acid interactions
Other lipoxygenases-8-LO, 12-LO and 15-LO-make other eicosanoid-like products. To act, 5-LO uses the nuclear-membrane enzyme 5- ... Phinney SD, Odin RS, Johnson SB, Holman RT (March 1990). "Reduced arachidonate in serum phospholipids and cholesteryl esters ... Lipoxygenase oxidation removes no C=C double bonds and leads to the LK. After oxidation, the eicosanoids are further modified, ... DGLA and EPA compete with AA for access to the cyclooxygenase and lipoxygenase enzymes. So the presence of DGLA and EPA in ...
... inclusion of dietary arachidonate and docosahexaenoate leads to increased brain levels of the corresponding N-acylethanolamines ... role of vanilloid receptors and lipoxygenases". The Journal of Neuroscience. 23 (10): 4127-4133. doi:10.1523/JNEUROSCI.23-10- ... 86 (15-16): 615-623. doi:10.1016/j.lfs.2009.06.003. PMC 2848893. PMID 19527737. Pacher P, Bátkai S, Kunos G (September 2006). " ... 106 (15): 6375-6380. Bibcode:2009PNAS..106.6375K. doi:10.1073/pnas.0901515106. PMC 2669397. PMID 19307565. Oddi S, Fezza F, ...
The eicosanoids formed in the ALOX12 (i.e. arachidonate-12-lipoxygnease) pathway of arachidonic acid metabolism, 12S- ... April 2012). "Spinal 12-lipoxygenase-derived hepoxilin A3 contributes to inflammatory hyperalgesia via activation of TRPV1 and ... 27 (3): 200-15. doi:10.1016/j.smim.2015.03.004. PMC 4515371. PMID 25857211. Macpherson LJ, Dubin AE, Evans MJ, Marr F, Schultz ... May 2013). "Systematic analysis of rat 12/15-lipoxygenase enzymes reveals critical role for spinal eLOX3 hepoxilin synthase ...
Brock TG (December 2005). "Regulating leukotriene synthesis: the role of nuclear 5-lipoxygenase" (PDF). Journal of Cellular ... The endocannabinoid system, broadly speaking, includes: The endogenous arachidonate-based lipids, anandamide (N- ... 45 (15): 4720-6. doi:10.1021/bi060163l. PMC 1538545. PMID 16605240. Pazos MR, Núñez E, Benito C, Tolón RM, Romero J (June 2005 ... 15: 747229. doi:10.3389/fnins.2021.747229. PMC 8581450. PMID 34776851. Baggelaar MP, Maccarrone M, van der Stelt M (July 2018 ...
"Depletion of phospholipid hydroperoxide glutathione peroxidase up-regulates arachidonate metabolism by 12S-lipoxygenase and ... untranslated region of human glutathione peroxidase 4 influences lipoxygenase metabolism". Blood Cells Mol. Dis. 29 (2): 174-8 ... "Glutathione peroxidase 4 senses and translates oxidative stress into 12/15-lipoxygenase dependent- and AIF-mediated cell death ...
... amines by the lipoxygenase pathway". European Journal of Biochemistry. 267 (7): 2000-7. doi:10.1046/j.1432-1327.2000.01203.x. ... inclusion of dietary arachidonate and docosahexaenoate leads to increased brain levels of the corresponding N-acylethanolamines ... or its linoleoyl analogues by lipoxygenase action. N-Docosahexaenoyl ethanolamine (DHEA: C24H37NO2; 22:6, ω-3), or Anandamide ( ... 4 can also be oxygenated via lipoxygenase (LOX) or cyclooxygenase (COX), to produce ethanolamide oxylipins, like prostaglandin ...
WikiGenes - ALOX15 - arachidonate 15-lipoxygenase
Arachidonate 15-lipoxygenase in human corneal epithelium and 12- and 15-lipoxygenases in bovine corneal epithelium: comparison ... Biochemical and immunohistochemical evidence for selective expression of novel epithelial lipoxygenases. Shannon, V.R., ... Arachidonate 15-lipoxygenase in human corneal epithelium and 12- and 15-lipoxygenases in bovine corneal epithelium: comparison ... with other bovine 12-lipoxygenases .. *Human tracheal epithelial cells contain an arachidonate 15-lipoxygenase, while the ...
Inactivation of the ferroptosis regulator Gpx4 triggers acute renal failure in mice
Arachidonate 12-Lipoxygenase * Arachidonate 15-Lipoxygenase Grant support * P01 HL114453/HL/NHLBI NIH HHS/United States ... 15 81377 Munich, Germany.. *3 Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, ... 15 81377 Munich, Germany.. *11 Institute of Pathology, Technische Universität München, Ismaningerstr. 22 81675 Munich, Germany. ...
Molecules | Free Full-Text | Interactive Effects of Light and Melatonin on Biosynthesis of Silymarin and Anti-Inflammatory...
Anti-inflammatory activities were also studied and highest percent inhibition was recorded against 15-lipoxygenase (15-LOX) for ... COX-2, phospholipase A2 and lipoxygenases (eicosanoid generating enzymes), thereby decreasing leukotrienes and prostanoid ... Arachidonate 15-Lipoxygenase; sPLA2: phospholipase A2; COX: cyclooxygenase. Values are means ± SD from three replicates. ... Anti-inflammatory and anti-arthritic activities of silymarin acting through inhibition of 5-lipoxygenase. Phytomedicine 2000, 7 ...
Exploration of the Potential Mechanisms of Lingqihuangban Granule for Treating Diabetic Retinopathy Based on Network...
15 Suppl 2: p. 65-71.. *Liu, L., et al., Lycium barbarum polysaccharides protected human retinal pigment epithelial cells ... 512(2-3): p. 105-15.. *Kimura, I., Medical benefits of using natural compounds and their derivatives having multiple ... 15]. However, the therapeutic effects of the remaining herbs on DR, such as Codonopsis pilosula, Semen Cuscutae and Cistanche, ... 15 for Ligusticum wallichii, 17 for Salvia miltiorrhiza and 13 for Codonopsis pilosula, for a total of 130 bioactive components ...
Science Clips - Volume 12, Issue 23, July 14, 2020
M1 macrophages exhibited preferential induction of arachidonate 5-lipoxygenase activating protein (ALOX5AP), whereas M2 ... arachidonate 15-lipoxygenase (ALOX15). Polarization of macrophages in vitro differentially induced ALOX5AP in M1 macrophages or ... Of the men found HIV positive, and 13.0% were aged 15 to 24 years, 60.3% were aged 25 to 39, 20.9% were aged 40 to 49 and 5.8% ... Here, we showed in 15 healthy young adults that VZV-specific B and CD4 T cell responses are detectable in bone marrow (BM) and ...
NIOSHTIC-2 Search Results - Full View
GSE26928 CENTR MEMORY VS CXCR5 POS CD4 TCELL DN
Robert Duvoisin - Publications - Oregon Health & Science University
Arachidonate 15-Lipoxygenase 100% * Proton-Motive Force 86% * Cell Culture Techniques 49% ... Hirano, A. A., Hack, I., Wässle, H. & Duvoisin, R. M., May 22 2000, In: Journal of Comparative Neurology. 421, 1, p. 80-94 15 p ... Francesconi, A. & Duvoisin, R. M., Feb 15 2004, In: Journal of Neuroscience Research. 75, 4, p. 472-479 8 p.. Research output: ... Francesconi, A. & Duvoisin, R. M., Mar 15 2002, In: Journal of Neuroscience. 22, 6, p. 2196-2205 10 p.. Research output: ...
Charles S. Craik, PhD | UCSF Macromolecular Structure Group
A Primary Determinant for Lipoxygenase Postional Specificity. Nature 354, 149-152. A Primary Determinant for Lipoxygenase ... Arachidonate 15-lipoxygenase from human leukocytes: purification and structural homology to other mammalian lipoxygenases.. ... omega-6 lipoxygenase) from human leukocytes. Purification and structural homology to other mammalian lipoxygenases.. The ... Arachidonate 15-lipoxygenase from human eosinophil-enriched leukocytes: partial purification and properties.. Biochemical and ...
Different roles of 12/15-lipoxygenase in diabetic large and small fiber peripheral and autonomic neuropathies<...
Arachidonate 15 Lipoxygenase Medicine and Dentistry 100% * Lipoxygenases Chemistry 90% * Fiber Chemistry 70% ... 12/15-lipoxygenase gene deficiency did not affect weight gain or blood glucose concentrations. Diabetic wild-type mice ... 12/15-lipoxygenase gene deficiency did not affect weight gain or blood glucose concentrations. Diabetic wild-type mice ... 12/15-lipoxygenase gene deficiency did not affect weight gain or blood glucose concentrations. Diabetic wild-type mice ...
Find Research outputs - University of Canberra Research Portal
Pharmacological inhibition of arachidonate 15-lipoxygenase protects human spermatozoa against oxidative stress. Walters, J. L. ... Huxtable, A., Millar, L., Love, P., Bell, C. & Whelan, J., May 2018, In: Nutrients. 10, 5, p. 1-15 15 p., 545.. Research output ... Prosser, B., Flinders, M., Jennings, W., Renwick, A., Spada, P., Stoker, G. & Ghose, K., 15 Mar 2018, In: Contemporary Politics ... Bainbridge, J., Nov 2018, In: Participations: journal of audience and reception studies. 15, 2, p. 365-375 11 p.. Research ...
91; was see under ARACHIDONATE LIPOXYGENASES 1987-90. History Note:. 91(87); was see under ARACHIDONATE LIPOXYGENASES 1987-90. ... Arachidonate 15-Lipoxygenase - Preferred Concept UI. M0001647. Scope note. An enzyme that catalyzes the oxidation of ... Arachidonate 15-Lipoxygenase Entry term(s). 15 Lipoxygenase 15-Lipoxygenase 15-Lipoxygenase, Arachidonate 15-Lipoxygenase, ... Arachidonate 15-lipoxygenase Entry term(s):. 15 Lipoxygenase. 15-Lipoxygenase. 15-Lipoxygenase, Arachidonate. 15-Lipoxygenase, ...
Code System Concept
DR97 766 protein (Pseudomonas aeruginosa) - STRING interaction network
Arachidonate 15-lipoxygenase; Exhibits lipoxin synthase activity as part of a bacterial evasion strategy to suppress the host ... Arachidonate 15-lipoxygenase; Exhibits lipoxin synthase activity as part of a bacterial evasion strategy to suppress the host ... Arachidonate 15-lipoxygenase; Exhibits lipoxin synthase activity as part of a bacterial evasion strategy to suppress the host ...
Cell atlas - ALOX15 - The Human Protein Atlas
Identification of 15-hydroxy-11,12-epoxyeicosatrienoic acid as a vasoactive 15-lipoxygenase metabolite in rabbit aorta -...
ISMARA results: FOXA2 FOXJ3
Cardiovascular Disease Research, Cardiovascular ELISA Kits for Research Use| TZYBIOTECH
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Pesquisa | Portal Regional da BVS
EOS highly expressed arachidonate 15-lipoxygenase (ALOX15), and EOS deficiency largely repressed ALOX15 expression in lungs. ... At times of 15 min, 30 min, and 60 min, bacteria adhesion on SBS-functionalized surfaces is reduced relative to the control ... 15. Genetic variant ABCC1 rs45511401 is associated with increased response to statins in patients with familial ... Frequencies of HLA-B*15:02 and HLA-B*58:01 were highest across Asia, which has important implications for the risk of severe ...
Vickie R Shannon | Cancer Research and Molecular Mechanisms | Sci Forschen
Induction of epithelial arachidonate 12-lipoxygenase at active sites of inflammatory bowel disease. Am J Physiol 264(1 Pt 1): ... Induction of arachidonate 12-lipoxygenase at active sites of inflammatory bowel disease. Am J Physoil (Gastorinterol) 264:G104- ... Selective expression of an arachidonate 12-lipoxygenase by pancreatic islet beta-cells. Am J Physiol 263(5 Pt 1):E828-36, 11/ ... Related expression of arachidonate 12- and 15-lipoxygenases in animal and human lung tissue. Am J Physiol 261(6 Pt 1):L399-405 ...
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November | 2018 | Wee1 signals
... experiments confirmed a critical role for FcαRI in the negative regulation of macrophage function Arachidonate 15-lipoxygenase ... The CRTH2 agonists PGD2 and 15dPGJ2 increase the expression of CR3 (CD11b) on eosinophils and basophils via CRTH2.[15, 27] ... As large numbers of allergens possess enzymatic activities [14, 15], we examined tryptic, chymotryptic, metalloproteinase and ...
CDC Science Clips
Multi-walled carbon nanotubes induce arachidonate 5-lipoxygenase expression and enhance the polarization and function of M1 ... M1 macrophages exhibited preferential induction of arachidonate 5-lipoxygenase activating protein (ALOX5AP), whereas M2 ... Inhal Toxicol 2020 32 (1) 1-15 Abstract. Objective: In this study, we compared in vitro and in vivo bioactivity of nitrogen- ... macrophages had a high level expression of arachidonate 15-lipoxygenase (ALOX15). Polarization of macrophages in vitro ...
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February | 2019 | P450 Inhibitors
Pam2Cys.125 Lipopeptides were able to Arachidonate 15-lipoxygenase induce specific CD8+ T-cell responses in HLA-A2 transgenic ... 15] Progression of APS-related renal TMA to end-stage kidney disease (ESKD) has been reported in a limited number of cases,[14 ... However, there was more progression of PPF in males (15, 8.5%) compared with females (9, 5.1%). The high number of females with ... Since L-plastin is an actin bundling protein which may stabilize actin filaments 15, we next asked whether L-plastin ...
Arachidonic acidALOX15MetabolismPathwayInhibitionInhibitorsIC50HETEAcidGeneMolecularSelectiveHumanJournalInitialInhibitorALOX5APProteinIncluding arachidonate and docosahexanoate-derived metabolitesBiosynthesisProstaglandin2020HumansCyclooxygenaseAntagonistsGeneInducesLipoxinProteinsReceptorEnzymesPlateletsMetabolitesRegulation1999MRNAVitroInflammationLipidsAutacoids2021LymphocytesInflammatoryImmunolAnnexinEpidermalSubstrateMembraneDiabeticStromal
- Up-regulation of 12/15-lipoxygenase, which converts arachidonic acid to 12(S)- and 15(S)-hydroxyeicosatetraenoic acids, causes impaired cell signaling, oxidative-nitrosative stress, and inflammation. (uthscsa.edu)
- An enzyme that catalyzes the oxidation of arachidonic acid to yield 15-hydroperoxyarachidonate (15-HPETE) which is rapidly converted to 15-hydroxy-5,8,11,13-eicosatetraenoate (15- HETE ). (bvsalud.org)
- The renal cortex does not metabolize arachidonic acid through the lipoxygenase pathway. (wustl.edu)
- 5-Lipoxygenase, Human, Recombinant, S. frugiperda E.C 126.96.36.199, catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. (sigmaaldrich.com)
- Arachidonate 12-lipoxygenase (12-LOX) converts arachidonic acid to 12(S)-(HETE). (sigmaaldrich.com)
- Prometastatic metabolite of arachidonic acid via the 12-lipoxygenase pathway that is expressed in many tumor cell lines. (sigmaaldrich.com)
- Recently, 14,15-HxA3 and 14,15-HxB3 have been defined as arachidonic acid derivatives that are produced by a different metabolic pathway than HxA3, HxB3, HxA4, or HxB4 and differ from the aforementioned hepoxilins in the positions of their hydroxyl and epoxide residues. (wikipedia.org)
- First, molecular oxygen (O 2 ) is added to carbon 12 of arachidonic acid (i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid) and concurrently the 8 Z double bond in this arachidonate moves to the 9 E position to form the intermediate product, 12 S -hydroperoxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (i.e. 12 S -hydroperoxyeicosatetraenoic acid or 12 S -HpETE). (wikipedia.org)
- Basil may be a useful antiinflammatory agent which block both cyclooxygenase and lipoxygenase pathways of arachidonic acid metabolism. (raysahelian.com)
- M1 macrophages exhibited preferential induction of arachidonate 5-lipoxygenase activating protein (ALOX5AP), whereas M2 macrophages had a high level expression of arachidonate 15-lipoxygenase (ALOX15). (cdc.gov)
- Thus, there is regional metabolism of arachidonate to monohydroxy eicosanoids in the kidney. (wustl.edu)
- Collectively, these landmark discoveries provided initial insight into the COX pathway of arachidonate metabolism. (medscape.com)
- The results of antiinflammatory activity support the dual inhibition of arachidonate metabolism as indicated by its activity in inflammation models that are insensitive to selective cyclooxygenase inhibitors. (raysahelian.com)
- Obesity is associated with increased expression of 5-lipoxygenase (5-LO) pathway and increased Leukotrienes (LTs) production has been observed in obese asthma patients. (utmb.edu)
- The 14,15-HxA3 and 14,15-HxB3 non-classical eicosanoids are distinguished from the aforementioned hepoxilins in that they are formed by a different metabolic pathway and differ in the positioning of their epoxide and hydroxyl residues. (wikipedia.org)
- Inhibition of 12/15-LOX activity and ceramide pattern in the murine brain. (edu.pl)
- This should be considered in the selection of endpoints for future clinical trials of 12/15-lipoxygenase inhibitors. (uthscsa.edu)
- A cell-permeable, highly potent, selective, active site directed, mixed type inhibitor of lipoxygenase-12/15 (IC50 = 200 nM for human). (sigmaaldrich.com)
- Enzima que cataliza la oxidación del ácido araquidónico para formar 15-hidroperoxiaraquidonato (15-HPETE), que es convertido rápidamente en 15-hidroxi-5,8,11,13-eicosatetraenoato (15-HETE). (bvsalud.org)
- Diabetic wild-type mice displayed increased sciatic nerve 12/15-lipoxygenase and 12(S)-hydroxyeicosatetraenoic acid levels. (uthscsa.edu)
- The mass spectra matched previously published mass spectra for 12-hydroxy-5,8,10,14-eicosatetraeinoic acid and 15-hydroxy-5,8,11,13-eicosatetraenoic acid. (wustl.edu)
- 12/15-lipoxygenase gene deficiency did not affect weight gain or blood glucose concentrations. (uthscsa.edu)
- Current molecular medicine , 15 (7), 598-605. (utmb.edu)
- Biochemical and immunohistochemical evidence for selective expression of novel epithelial lipoxygenases. (wikigenes.org)
- Arachidonate 15-lipoxygenase in human corneal epithelium and 12- and 15-lipoxygenases in bovine corneal epithelium: comparison with other bovine 12-lipoxygenases. (wikigenes.org)
- Journal of Immunotoxicology Downloaded from informahealthcare.com by CDC Information Center on 03/30/15 inflammatory response (Maestrelli et al. (cdc.gov)
- Amplification was performed with an initial incubation at 95 °C for 15 min, followed by 30 cycles of 94 °C for 1 min, 55 °C for 1 min, 72 °C for 90 seconds and a final extension at 72 °C for 10 min. (jakenzyme.com)
- An antioxidant found in the creosote bush, Larrea divaricata, it is a potent lipoxygenase inhibitor that interferes with arachidonic acid metabolism. (mcw.edu)
- Sodium benzoate is an organic sodium salt resulting from the replacement of the proton from the carboxy group of benzoic acid by a sodium ion.It has a role as an antimicrobial food preservative, a drug allergen, an EC 188.8.131.52 (arachidonate 15-lipoxygenase) inhibitor, an EC 184.108.40.206 (triacylglycerol lipase) inhibitor, an algal metabolite, a human xenobiotic metabolite and a plant metabolite. (yahoo.com)
- It has a role as a plant metabolite, an EC 220.127.116.11 (arachidonate 15-lipoxygenase) inhibitor, an EC 18.104.22.168 (glutathione transferase) inhibitor, an EC 22.214.171.124 (arachidonate 5-lipoxygenase) inhibitor, an antioxidant and an EC 126.96.36.199 (histone deacetylase) inhibitor. (nih.gov)
- 5-Lipoxygenase activating protein (ALOX5AP) gene variants associate with the presence of xanthomas in familial hypercholesterolemia. (cdc.gov)
- the enzymes 5-lipoxygenase (5-LO, ALOX5) with its activating protein, FLAP (ALOX5AP), LTA(4) hydrolase and LTC(4) synthase, as well as the receptors for leukotriene B(4) (BLT(1) and BLT(2)) and cysteinyl-leukotrienes (CysLT(1) and CysLT(2)), respectively. (nih.gov)
- The arachidonate 5-lipoxygenase activating protein gene polymorphism is associated with the risk of scleroderma-related interstitial lung disease: a multicentre European Scleroderma Trials and Research group (EUSTAR) study. (cdc.gov)
- Ischemic stroke risk in a southeastern Chinese population: Insights from 5-lipoxygenase activating protein and phosphodiesterase 4D single-nucleotide polymorphisms. (cdc.gov)
- Helgadottir and colleagues 44 showed that a promoter haplotype comprising 4 linked polymorphisms in the 5-LO activating peptide (an accessory protein that facilitates presentation of substrate arachidonate to 5-LO) confers an approximately 2-fold increased risk of myocardial infarction (MI) and stroke in an Icelandic population. (healthfully.org)
- Identification of a naturally occurring peroxidase-lipoxygenase fusion protein. (vanderbilt.edu)
- Gür ZT, Çalışkan B, Banoglu E. Drug discovery approaches targeting 5-Lipoxygenase-Activating Protein (FLAP) for inhibition of cellular leukotriene biosynthesis. (eurekaselect.com)
Including arachidonate and docosahexanoate-derived metabolites2
- Consistent with this finding, another study profiling lipid autacoids in diabetic vitreous humor from 47 individuals undergoing vitreoretinal surgery showed a direct diabetes-specific effect on vitreous lipid metabolites, including arachidonate and docosahexanoate-derived metabolites. (medscape.com)
- This study is the first to demonstrate diabetes-specific changes in vitreous lipid autacoids including arachidonate and docosahexanoate-derived metabolites indicating an increase in inflammatory versus anti-inflammatory lipid mediators that correlated with increased levels of inflammatory and angiogenic proteins, further supporting the notion that inflammation plays a role the pathogenesis of this disease. (diabetesjournals.org)
- mRNA expression of enzymes mediating the biosynthesis of eicosanoids, including cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1), but not 12/15-lipoxygenase (LOX), was upregulated in liver macrophages 3-24 h after LPS, with no effect on lung macrophages. (nih.gov)
- The inflammatory environment within the atherosclerotic lesion stimulates the 5-lipoxygenase pathway of arachidonic acid metabolism, leading to the biosynthesis of the potent lipid inflammatory mediators leukotrienes. (nih.gov)
- 40 Furthermore, work from Serhan's group shows that acetylation of COX-2 by low-dose aspirin leads to its biosynthesis of 15 R -hydroxyeicosatetraenoic acid. (healthfully.org)
- On the mechanism of biosynthesis of 5- and 15-series leukotrienes. (vanderbilt.edu)
- COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. (thermofisher.com)
- The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). (thermofisher.com)
- Sequential high-performance liquid chromatographic analyses of islet-derived metabolites of 3 H-labeled arachidonate in both reversed and normal phases with 14 C-labeled internal standards have demonstrated synthesis by the islets of the cyclooxygenase products prostaglandin E 2 , prostaglandin F 2α , thromboxane B 2 and 12-hydroxyheptadecatrienoic acid as well as the lipoxygenase product 12-hydroxyeicosatetraenoic acid (12-HETE). (wustl.edu)
- Sequential high-performance liquid chromatographic analyses of islet-derived metabolites of 3H-labeled arachidonate in both reversed and normal phases with 14C-labeled internal standards have demonstrated synthesis by the islets of the cyclooxygenase products prostaglandin E2, prostaglandin F2α, thromboxane B2 and 12-hydroxyheptadecatrienoic acid as well as the lipoxygenase product 12-hydroxyeicosatetraenoic acid (12-HETE). (wustl.edu)
- 1) prostaglandin (PG) endoperoxide synthase (cyclooxygenase [COX]), (2) lipoxygenase (LO), (3) P450 epoxygenase, and (4) (nonenzymatic) isoprostane synthesis. (healthfully.org)
- 2020 May 15;295(20):6946-6957. (uiowa.edu)
- 15-LOX-2, which is expressed in epithelial cells and leukocytes, has different substrate specificity in the humans and mice, therefore, the role of them in mammals has not been established. (nih.gov)
- These studies establish that cells intrinsic to pancreatic islets synthesize a clearly characterized profile of arachidonate lipoxygenase and cyclooxygenase products. (wustl.edu)
- Inhibiting the 5-lipoxygenase pathway through either leukotriene synthesis inhibitors or leukotriene receptor antagonists in experimental models of atherosclerosis has however generated contradictory results. (nih.gov)
- Molecular structure and function of the porcine arachidonate 12-lipoxygenase gene. (glycosciences.de)
- Previously, we found that c-Jun induction was required in epidermal growth factor (EGF) induced human 12(S)-lipoxygenase (12(S)-LOX) gene expression in human epidermoid carcinoma A431 cells. (ncl.edu.tw)
- 2001). Stimulation of neuroendocrine axes, such as hypothalamus-pituitary-adrenal Arachidonate 15-lipoxygenase (HPA) and hypothalamus-pituitary-gonads (HPG) induces secretion of hormones which have profound effects on immune response (Besedovsky et al. (pafr-signal.com)
- 15. Conditional expression of human 15-lipoxygenase-1 in mouse prostate induces prostatic intraepithelial neoplasia: the FLiMP mouse model. (nih.gov)
- We identified 15-epi lipoxin A4, a stable lipoxin isoform derived from aspirin treatment, as potentially beneficial in the resolution of tendon inflammation. (ox.ac.uk)
- The 3'UTR of 15-LOX mRNA contains tandem repeat sequences, referred to as 'DICE' for 'DIfferentiation Control Element' which specifically bind to regulatory proteins, formerly called LOX-BP. (cnr.it)
- 6. 15-lipoxygenase-1 expression upregulates and activates insulin-like growth factor-1 receptor in prostate cancer cells. (nih.gov)
-  Glucocorticoids are corticosteroids that bind to the glucocorticoid receptor  that is present in almost every vertebrate animal cell. (wikipedia.org)
- Inflammation's role in development of atheromatous plaque was reported in Scientific American in 1978-jk] Although 12-LO and 15-LO appear to contribute to LDL oxidation, the data supporting the proatherogenic role of these enzymes are inconsistent. (healthfully.org)
- The first method deter-mined the effect of clove oil against cyclo-oxygenase and lipoxygenase enzymes in platelets. (org.pk)
- F. Enzymes The highly active enzyme that catalyzes lipid oxidation, lipoxygenase, is contained in beans and cereals. (firnm.net)
- Platelets contaminating the isolated islet preparations have been excluded as the source of the identified arachidonate metabolites. (wustl.edu)
- Synthesis of the identified metabolites from endogenous arachidonate has also been quantitated with the use of deuterated internal standards, capillary column gas chromatographic analyses, and negative ion-chemical ionization mass spectrometric measurements. (wustl.edu)
- The relative abundances of metabolites derived from exogenous, radiolabeled arachidonate versus endogenous precursor differed considerably, and 12-HETE was by far the most abundant of these metabolites synthesized from endogenous arachidonate. (wustl.edu)
- 1995 . Studies on the regulation and localization of 5-lipoxygenase in human B-lymphocytes. (nih.gov)
- 17. Regulation of 15-lipoxygenase expression and mucus secretion by IL-4 in human bronchial epithelial cells. (nih.gov)
- Structure and regulation of arachidonate 11R-lipoxygenase = Arahhidoon. (digar.ee)
- 1999 . The arachidonate 12/15 lipoxygenases. (nih.gov)
- J Bacteriol 1999, 181:1203-1210.PubMed 15. (sirtsignaling.com)
- This binding specifically inhibits 15-LOX mRNA translation. (cnr.it)
- In vivo and in vitro lipid peroxidation of arachidonate esters: the effect of fish oil omega-3 lipids on product distribution. (vanderbilt.edu)
- The 12-,15-, and 5-LOs all have key roles in inflammation, and the role of each in atherosclerosis has been examined. (healthfully.org)
- Erythroid 15-lipoxygenase (15-LOX) synthesized in the early stages of erytropoiesis catalyses the degradation of lipids and is an important factor responsible for the degradation of mitochondria during reticulocyte maturation. (cnr.it)
- Vitreous lipids included lipoxygenase (LO)- and cytochrome P450 epoxygenase (CYP)-derived eicosanoids. (diabetesjournals.org)
- Concurrently, lowered levels of anti-inflammatory autacoids including 15-HETE, resolvins, neuroprotectin D1 and epoxytrienoic acids were observed in the diabetic vitreous humor compared with nondiabetic vitreous. (medscape.com)
- 50: 10-15, Mar. 2021. (bvsalud.org)
- Commercially available extracts from Boswellia serrata resin used as anti-inflammatory drugs or phytonutrients show paradoxical concentration-dependent potentiating and inhibitory actions on 5-lipoxygenase (5-LO) product synthesis in stimulated PMNs. (nih.gov)
- Journal of Immunotoxicology Downloaded from informahealthcare.com by CDC Information Center on 03/30/15 inflammatory response (Maestrelli et al. (cdc.gov)
- Nat Rev Immunol 15:511-52326139350. (nih.gov)
- 4. Distinct effects of annexin A7 and p53 on arachidonate lipoxygenation in prostate cancer cells involve 5-lipoxygenase transcription. (nih.gov)
- On the role of molecular oxygen in lipoxygenase activation: comparison and contrast of epidermal lipoxygenase-3 with soybean lipoxygenase-1. (vanderbilt.edu)
- Site-directed mutagenesis studies on the iron-binding domain and the determinant for the substrate oxygenation site of porcine leukocyte arachidonate 12-lipoxygenase. (glycosciences.de)
- The prostaglandins are synthesized in the cell membrane by the cleavage of arachidonate from the phospholipids that make up the membrane. (fingerwaverecords.com)
- Vitreous was recovered from 47 individuals undergoing vitreoretinal surgery: 16 had nonproliferative diabetic retinopathy (NPDR), 15 had proliferative diabetic retinopathy, 7 had retinal detachments, and 9 had epiretinal membranes. (diabetesjournals.org)
- 15. A Stromal Lysolipid-Autotaxin Signaling Axis Promotes Pancreatic Tumor Progression. (nih.gov)