An enzyme of the oxidoreductase class primarily found in PLANTS. It catalyzes reactions between linoleate and other fatty acids and oxygen to form hydroperoxy-fatty acid derivatives.
An enzyme that catalyzes the oxidation of arachidonic acid to yield 5-hydroperoxyarachidonate (5-HPETE) which is rapidly converted by a peroxidase to 5-hydroxy-6,8,11,14-eicosatetraenoate (5-HETE). The 5-hydroperoxides are preferentially formed in leukocytes.
An enzyme that catalyzes the oxidation of arachidonic acid to yield 12-hydroperoxyarachidonate (12-HPETE) which is itself rapidly converted by a peroxidase to 12-hydroxy-5,8,10,14-eicosatetraenoate (12-HETE). The 12-hydroperoxides are preferentially formed in PLATELETS.
Compounds that bind to and inhibit that enzymatic activity of LIPOXYGENASES. Included under this category are inhibitors that are specific for lipoxygenase subtypes and act to reduce the production of LEUKOTRIENES.
An enzyme that catalyzes the oxidation of arachidonic acid to yield 15-hydroperoxyarachidonate (15-HPETE) which is rapidly converted to 15-hydroxy-5,8,11,13-eicosatetraenoate (15-HETE). The 15-hydroperoxides are preferentially formed in NEUTROPHILS and LYMPHOCYTES.
Enzymes catalyzing the oxidation of arachidonic acid to hydroperoxyarachidonates. These products are then rapidly converted by a peroxidase to hydroxyeicosatetraenoic acids. The positional specificity of the enzyme reaction varies from tissue to tissue. The final lipoxygenase pathway leads to the leukotrienes. EC 1.13.11.- .
An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.
A lipoxygenase metabolite of ARACHIDONIC ACID. It is a highly selective ligand used to label mu-opioid receptors in both membranes and tissue sections. The 12-S-HETE analog has been reported to augment tumor cell metastatic potential through activation of protein kinase C. (J Pharmacol Exp Ther 1995; 274(3):1545-51; J Natl Cancer Inst 1994; 86(15):1145-51)
A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.
A potent lipoxygenase inhibitor that interferes with arachidonic acid metabolism. The compound also inhibits formyltetrahydrofolate synthetase, carboxylesterase, and cyclooxygenase to a lesser extent. It also serves as an antioxidant in fats and oils.
Scaffolding proteins that play an important role in the localization and activation of 5-LIPOXYGENASE.
A dual inhibitor of both cyclooxygenase and lipoxygenase pathways. It exerts an anti-inflammatory effect by inhibiting the formation of prostaglandins and leukotrienes. The drug also enhances pulmonary hypoxic vasoconstriction and has a protective effect after myocardial ischemia.
The major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990)
Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes.
Eighteen-carbon essential fatty acids that contain two double bonds.
An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.
Phospholipases that hydrolyze one of the acyl groups of phosphoglycerides or glycerophosphatidates.
A 20-carbon unsaturated fatty acid containing 4 alkyne bonds. It inhibits the enzymatic conversion of arachidonic acid to prostaglandins E(2) and F(2a).
Phospholipases that hydrolyze the acyl group attached to the 2-position of PHOSPHOGLYCERIDES.
A class of compounds named after and generally derived from C20 fatty acids (EICOSANOIC ACIDS) that includes PROSTAGLANDINS; LEUKOTRIENES; THROMBOXANES, and HYDROXYEICOSATETRAENOIC ACIDS. They have hormone-like effects mediated by specialized receptors (RECEPTORS, EICOSANOID).
(2S-(2 alpha,3 beta(1E,3E,5Z,8Z)))-3-(1,3,5,8-Tetradecatetraenyl)oxiranebutanoic acid. An unstable allylic epoxide, formed from the immediate precursor 5-HPETE via the stereospecific removal of a proton at C-10 and dehydration. Its biological actions are determined primarily by its metabolites, i.e., LEUKOTRIENE B4 and cysteinyl-leukotrienes. Alternatively, leukotriene A4 is converted into LEUKOTRIENE C4 by glutathione-S-transferase or into 5,6-di-HETE by the epoxide-hydrolase. (From Dictionary of Prostaglandins and Related Compounds, 1990)
A non-steroidal anti-inflammatory agent (NSAID) that inhibits the enzyme cyclooxygenase necessary for the formation of prostaglandins and other autacoids. It also inhibits the motility of polymorphonuclear leukocytes.
Enzyme complexes that catalyze the formation of PROSTAGLANDINS from the appropriate unsaturated FATTY ACIDS, molecular OXYGEN, and a reduced acceptor.
20-carbon saturated monocarboxylic acids.
A doubly unsaturated fatty acid, occurring widely in plant glycosides. It is an essential fatty acid in mammalian nutrition and is used in the biosynthesis of prostaglandins and cell membranes. (From Stedman, 26th ed)
A group of LEUKOTRIENES; (LTC4; LTD4; and LTE4) that is the major mediator of BRONCHOCONSTRICTION; HYPERSENSITIVITY; and other allergic reactions. Earlier studies described a "slow-reacting substance of ANAPHYLAXIS" released from lung by cobra venom or after anaphylactic shock. The relationship between SRS-A leukotrienes was established by UV which showed the presence of the conjugated triene. (From Merck Index, 11th ed)
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
A stable, physiologically active compound formed in vivo from the prostaglandin endoperoxides. It is important in the platelet-release reaction (release of ADP and serotonin).
(11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.
Compounds or agents that combine with cyclooxygenase (PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES) and thereby prevent its substrate-enzyme combination with arachidonic acid and the formation of eicosanoids, prostaglandins, and thromboxanes.
A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.
Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
The most common and most biologically active of the mammalian prostaglandins. It exhibits most biological activities characteristic of prostaglandins and has been used extensively as an oxytocic agent. The compound also displays a protective effect on the intestinal mucosa.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
An acridine derivative formerly widely used as an antimalarial but superseded by chloroquine in recent years. It has also been used as an anthelmintic and in the treatment of giardiasis and malignant effusions. It is used in cell biological experiments as an inhibitor of phospholipase A2.
A class of enzymes that catalyze the hydrolysis of phosphoglycerides or glycerophosphatidates. EC 3.1.-.
FATTY ACIDS in which the carbon chain contains one or more double or triple carbon-carbon bonds.
Physiologically active compounds found in many organs of the body. They are formed in vivo from the prostaglandin endoperoxides and cause platelet aggregation, contraction of arteries, and other biological effects. Thromboxanes are important mediators of the actions of polyunsaturated fatty acids transformed by cyclooxygenase.
The rate dynamics in chemical or physical systems.
A phospholipid derivative formed by PLATELETS; BASOPHILS; NEUTROPHILS; MONOCYTES; and MACROPHAGES. It is a potent platelet aggregating agent and inducer of systemic anaphylactic symptoms, including HYPOTENSION; THROMBOCYTOPENIA; NEUTROPENIA; and BRONCHOCONSTRICTION.
Long chain organic acid molecules that must be obtained from the diet. Examples are LINOLEIC ACIDS and LINOLENIC ACIDS.
A prostaglandin that is a powerful vasodilator and inhibits platelet aggregation. It is biosynthesized enzymatically from PROSTAGLANDIN ENDOPEROXIDES in human vascular tissue. The sodium salt has been also used to treat primary pulmonary hypertension (HYPERTENSION, PULMONARY).
A group of FLAVONOIDS characterized with a 4-ketone.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to the hexahydroxy alcohol, myo-inositol. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid, myo-inositol, and 2 moles of fatty acids.
Trihydroxy derivatives of eicosanoic acids. They are primarily derived from arachidonic acid, however eicosapentaenoic acid derivatives also exist. Many of them are naturally occurring mediators of immune regulation.
A 20-carbon-chain fatty acid, unsaturated at positions 8, 11, and 14. It differs from arachidonic acid, 5,8,11,14-eicosatetraenoic acid, only at position 5.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.
Eighteen-carbon essential fatty acids that contain three double bonds.
A constitutively-expressed subtype of prostaglandin-endoperoxide synthase. It plays an important role in many cellular processes.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.
The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS.
The conjugation product of LEUKOTRIENE A4 and glutathione. It is the major arachidonic acid metabolite in macrophages and human mast cells as well as in antigen-sensitized lung tissue. It stimulates mucus secretion in the lung, and produces contractions of nonvascular and some VASCULAR SMOOTH MUSCLE. (From Dictionary of Prostaglandins and Related Compounds, 1990)
An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Azoles of two nitrogens at the 1,2 positions, next to each other, in contrast with IMIDAZOLES in which they are at the 1,3 positions.
A cytosolic phospholipase A2 group that plays an important role in the release of free ARACHIDONIC ACID, which in turn is metabolized to PROSTAGLANDINS by the CYCLOOXYGENASE pathway and to LEUKOTRIENES by the 5-LIPOXYGENASE pathway.
A nonapeptide messenger that is enzymatically produced from KALLIDIN in the blood where it is a potent but short-lived agent of arteriolar dilation and increased capillary permeability. Bradykinin is also released from MAST CELLS during asthma attacks, from gut walls as a gastrointestinal vasodilator, from damaged tissues as a pain signal, and may be a neurotransmitter.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
A class of drugs designed to prevent leukotriene synthesis or activity by blocking binding at the receptor level.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Derivatives of PHOSPHATIDYLCHOLINES obtained by their partial hydrolysis which removes one of the fatty acid moieties.
Chemical agents that increase the permeability of biological or artificial lipid membranes to specific ions. Most ionophores are relatively small organic molecules that act as mobile carriers within membranes or coalesce to form ion permeable channels across membranes. Many are antibiotics, and many act as uncoupling agents by short-circuiting the proton gradient across mitochondrial membranes.
Fatty acid derivatives of glycerophosphates. They are composed of glycerol bound in ester linkage with 1 mole of phosphoric acid at the terminal 3-hydroxyl group and with 2 moles of fatty acids at the other two hydroxyl groups.
An enzyme found predominantly in platelet microsomes. It catalyzes the conversion of PGG(2) and PGH(2) (prostaglandin endoperoxides) to thromboxane A2. EC
Enzymes that catalyze reversibly the formation of an epoxide or arene oxide from a glycol or aromatic diol, respectively.
The process of converting an acid into an alkyl or aryl derivative. Most frequently the process consists of the reaction of an acid with an alcohol in the presence of a trace of mineral acid as catalyst or the reaction of an acyl chloride with an alcohol. Esterification can also be accomplished by enzymatic processes.
The physiologically active and stable hydrolysis product of EPOPROSTENOL. Found in nearly all mammalian tissue.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Synthetic compounds that are analogs of the naturally occurring prostaglandin endoperoxides and that mimic their pharmacologic and physiologic activities. They are usually more stable than the naturally occurring compounds.
An inducibly-expressed subtype of prostaglandin-endoperoxide synthase. It plays an important role in many cellular processes and INFLAMMATION. It is the target of COX2 INHIBITORS.
The prototypical analgesic used in the treatment of mild to moderate pain. It has anti-inflammatory and antipyretic properties and acts as an inhibitor of cyclooxygenase which results in the inhibition of the biosynthesis of prostaglandins. Aspirin also inhibits platelet aggregation and is used in the prevention of arterial and venous thrombosis. (From Martindale, The Extra Pharmacopoeia, 30th ed, p5)
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
An unstable intermediate between the prostaglandin endoperoxides and thromboxane B2. The compound has a bicyclic oxaneoxetane structure. It is a potent inducer of platelet aggregation and causes vasoconstriction. It is the principal component of rabbit aorta contracting substance (RCS).
An antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to an ethanolamine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and ethanolamine and 2 moles of fatty acids.
A group of fatty acids that contain 18 carbon atoms and a double bond at the omega 9 carbon.
Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Cell-surface receptors that bind LEUKOTRIENES with high affinity and trigger intracellular changes influencing the behavior of cells. The leukotriene receptor subtypes have been tentatively named according to their affinities for the endogenous leukotrienes LTB4; LTC4; LTD4; and LTE4.
A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)
A class of phenolic acids related to chlorogenic acid, p-coumaric acid, vanillic acid, etc., which are found in plant tissues. It is involved in plant growth regulation.
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
A group of 1,2-benzenediols that contain the general formula R-C6H5O2.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
A group of physiologically active prostaglandin endoperoxides. They are precursors in the biosynthesis of prostaglandins and thromboxanes. Most frequently encountered member of this group is the prostaglandin G2.
Important polyunsaturated fatty acid found in fish oils. It serves as the precursor for the prostaglandin-3 and thromboxane-3 families. A diet rich in eicosapentaenoic acid lowers serum lipid concentration, reduces incidence of cardiovascular disorders, prevents platelet aggregation, and inhibits arachidonic acid conversion into the thromboxane-2 and prostaglandin-2 families.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Established cell cultures that have the potential to propagate indefinitely.
Enzymes of the isomerase class that catalyze the oxidation of one part of a molecule with a corresponding reduction of another part of the same molecule. They include enzymes converting aldoses to ketoses (ALDOSE-KETOSE ISOMERASES), enzymes shifting a carbon-carbon double bond (CARBON-CARBON DOUBLE BOND ISOMERASES), and enzymes transposing S-S bonds (SULFUR-SULFUR BOND ISOMERASES). (From Enzyme Nomenclature, 1992) EC 5.3.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
A biologically active principle of SRS-A that is formed from LEUKOTRIENE D4 via a peptidase reaction that removes the glycine residue. The biological actions of LTE4 are similar to LTC4 and LTD4. (From Dictionary of Prostaglandins and Related Compounds, 1990)
Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A non-steroidal anti-inflammatory agent with antipyretic and antigranulation activities. It also inhibits prostaglandin biosynthesis.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A group of 16-carbon fatty acids that contain no double bonds.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The addition of an organic acid radical into a molecule.
A nonsteroidal anti-inflammatory agent with analgesic properties used in the therapy of rheumatism and arthritis.
A sarcoma derived from deep fibrous tissue, characterized by bundles of immature proliferating fibroblasts with variable collagen formation, which tends to invade locally and metastasize by the bloodstream. (Stedman, 25th ed)
Compounds based on benzeneacetamide, that are similar in structure to ACETANILIDES.
Enzymes that catalyze the formation of acyl-CoA derivatives. EC 6.2.1.
A calcium-independent phospholipase A2 group that may play a role in membrane phospholipid remodeling and homeostasis by controling the levels of PHOSPHATIDYLCHOLINE in mammalian cell membranes.
An enzyme localized predominantly within the plasma membrane of lymphocytes. It catalyzes the transfer of long-chain fatty acids, preferentially unsaturated fatty acids, to lysophosphatides with the formation of 1,2-diacylglycero-3-phosphocholine and CoA. EC
C22-unsaturated fatty acids found predominantly in FISH OILS.
GLYCEROPHOSPHOLIPIDS in which one of the two acyl chains is attached to glycerol with an ether alkenyl linkage instead of an ester as with the other glycerophospholipids.
The salts or esters of salicylic acids, or salicylate esters of an organic acid. Some of these have analgesic, antipyretic, and anti-inflammatory activities by inhibiting prostaglandin synthesis.
Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.
Cyclohexane ring substituted by one or more ketones in any position.
Organic compounds containing both the hydroxyl and carboxyl radicals.
An ethylmercury-sulfidobenzoate that has been used as a preservative in VACCINES; ANTIVENINS; and OINTMENTS. It was formerly used as a topical antiseptic. It degrades to ethylmercury and thiosalicylate.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Anti-inflammatory agents that are non-steroidal in nature. In addition to anti-inflammatory actions, they have analgesic, antipyretic, and platelet-inhibitory actions.They act by blocking the synthesis of prostaglandins by inhibiting cyclooxygenase, which converts arachidonic acid to cyclic endoperoxides, precursors of prostaglandins. Inhibition of prostaglandin synthesis accounts for their analgesic, antipyretic, and platelet-inhibitory actions; other mechanisms may contribute to their anti-inflammatory effects.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.
A class of cell surface leukotriene receptors with a preference for leukotriene B4. Leukotriene B4 receptor activation influences chemotaxis, chemokinesis, adherence, enzyme release, oxidative bursts, and degranulation in polymorphonuclear leukocytes. There are at least two subtypes of these receptors. Some actions are mediated through the inositol phosphate and diacylglycerol second messenger systems.
An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.
An unsaturated fatty acid that is the most widely distributed and abundant fatty acid in nature. It is used commercially in the preparation of oleates and lotions, and as a pharmaceutical solvent. (Stedman, 26th ed)
A subcategory of secreted phospholipases A2 that includes enzymes isolated from a variety of sources. The creation of this group is based upon similarities in the structural determinants of the enzymes including a negatively charged carboxy-terminal segment.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
Eighteen-carbon cyclopentyl polyunsaturated fatty acids derived from ALPHA-LINOLENIC ACID via an oxidative pathway analogous to the EICOSANOIDS in animals. Biosynthesis is inhibited by SALICYLATES. A key member, jasmonic acid of PLANTS, plays a similar role to ARACHIDONIC ACID in animals.
Elements of limited time intervals, contributing to particular results or situations.
A creeping annual plant species of the CUCURBITACEAE family. It has a rough succulent, trailing stem and hairy leaves with three to five pointed lobes.
A secreted phospholipase A2 subtype that contains a interfacial-binding region with specificity for PHOSPHATIDYLCHOLINE. This enzyme group may play a role in eliciting ARACHIDONIC ACID release from intact cellular membranes and from LOW DENSITY LIPOPROTEINS. Members of this group bind specifically to PHOSPHOLIPASE A2 RECEPTORS.
Dioxygenases that catalyze the peroxidation of methylene-interrupted UNSATURATED FATTY ACIDS.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A chemically diverse group of substances produced by various tissues in the body that cause slow contraction of smooth muscle; they have other intense but varied pharmacologic activities.
Compounds that inhibit the action of prostaglandins.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
Enzymes from the transferase class that catalyze the transfer of acyl groups from donor to acceptor, forming either esters or amides. (From Enzyme Nomenclature 1992) EC 2.3.
A group of physiologically active prostaglandin endoperoxides. They are precursors in the biosynthesis of prostaglandins and thromboxanes. The most frequently encountered member of this group is the prostaglandin H2.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A plant species of the genus SOLANUM, family SOLANACEAE. The starchy roots are used as food. SOLANINE is found in green parts.
Transport proteins that carry specific substances in the blood or across cell membranes.
(9 alpha,11 alpha,13E,15S)-9,11,15-Trihydroxyprost-13-en-1-oic acid (PGF(1 alpha)); (5Z,9 alpha,11,alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic acid (PGF(2 alpha)); (5Z,9 alpha,11 alpha,13E,15S,17Z)-9,11,15-trihydroxyprosta-5,13,17-trien-1-oic acid (PGF(3 alpha)). A family of prostaglandins that includes three of the six naturally occurring prostaglandins. All naturally occurring PGF have an alpha configuration at the 9-carbon position. They stimulate uterine and bronchial smooth muscle and are often used as oxytocics.
Benzene rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups.
Precursors in the biosynthesis of prostaglandins and thromboxanes from arachidonic acid. They are physiologically active compounds, having effect on vascular and airway smooth muscles, platelet aggregation, etc.
Proteins prepared by recombinant DNA technology.
A class of lipoproteins of small size (18-25 nm) and light (1.019-1.063 g/ml) particles with a core composed mainly of CHOLESTEROL ESTERS and smaller amounts of TRIGLYCERIDES. The surface monolayer consists mostly of PHOSPHOLIPIDS, a single copy of APOLIPOPROTEIN B-100, and free cholesterol molecules. The main LDL function is to transport cholesterol and cholesterol esters to extrahepatic tissues.
A stable prostaglandin endoperoxide analog which serves as a thromboxane mimetic. Its actions include mimicking the hydro-osmotic effect of VASOPRESSIN and activation of TYPE C PHOSPHOLIPASES. (From J Pharmacol Exp Ther 1983;224(1): 108-117; Biochem J 1984;222(1):103-110)
Mononuclear phagocytes derived from bone marrow precursors but resident in the peritoneum.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.
Phospholipids which have an alcohol moiety in ethereal linkage with a saturated or unsaturated aliphatic alcohol. They are usually derivatives of phosphoglycerols or phosphatidates. The other two alcohol groups of the glycerol backbone are usually in ester linkage. These compounds are widely distributed in animal tissues.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.
A dideoxynucleoside compound in which the 3'-hydroxy group on the sugar moiety has been replaced by a hydrogen. This modification prevents the formation of phosphodiester linkages which are needed for the completion of nucleic acid chains. The compound is an inhibitor of HIV replication, acting as a chain-terminator of viral DNA by binding to reverse transcriptase. Its principal side effect is nephrotoxicity. In vivo, dideoxyadenosine is rapidly metabolized to DIDANOSINE (ddI) by enzymatic deamination; ddI is then converted to dideoxyinosine monophosphate and ultimately to dideoxyadenosine triphosphate, the putative active metabolite.
A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.
Compounds that bind to and inhibit the action of 5-LIPOXYGENASE-ACTIVATING PROTEINS.
Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
One of the biologically active principles of SRS-A. It is generated from LEUKOTRIENE C4 after partial hydrolysis of the peptide chain, i.e., cleavage of the gamma-glutamyl portion. Its biological actions include stimulation of vascular and nonvascular smooth muscle, and increases in vascular permeability. (From Dictionary of Prostaglandins and Related Compounds, 1990)
A phenothiazine with actions similar to CHLORPROMAZINE. It is used as an antipsychotic and an antiemetic.
Cell surface proteins that bind eicosanoids with high affinity and trigger intracellular changes influencing the behavior of cells. Among the eicosanoid receptors are receptors for the prostaglandins, thromboxanes, and leukotrienes.
A group of alicyclic hydrocarbons with the general formula R-C5H9.
Derivatives of phosphatidic acid in which the hydrophobic regions are composed of two fatty acids and a polar alcohol is joined to the C-3 position of glycerol through a phosphodiester bond. They are named according to their polar head groups, such as phosphatidylcholine and phosphatidylethanolamine.
A flavoprotein enzyme that catalyzes the univalent reduction of OXYGEN using NADPH as an electron donor to create SUPEROXIDE ANION. The enzyme is dependent on a variety of CYTOCHROMES. Defects in the production of superoxide ions by enzymes such as NADPH oxidase result in GRANULOMATOUS DISEASE, CHRONIC.
Proteins, usually acting in oxidation-reduction reactions, containing iron but no porphyrin groups. (Lehninger, Principles of Biochemistry, 1993, pG-10)
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.
The space enclosed by the peritoneum. It is divided into two portions, the greater sac and the lesser sac or omental bursa, which lies behind the STOMACH. The two sacs are connected by the foramen of Winslow, or epiploic foramen.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
GLYCEROL esterified with FATTY ACIDS.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
7-Hydroxycoumarins. Substances present in many plants, especially umbelliferae. Umbelliferones are used in sunscreen preparations and may be mutagenic. Their derivatives are used in liver therapy, as reagents, plant growth factors, sunscreens, insecticides, parasiticides, choleretics, spasmolytics, etc.
Fatty acid esters of cholesterol which constitute about two-thirds of the cholesterol in the plasma. The accumulation of cholesterol esters in the arterial intima is a characteristic feature of atherosclerosis.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
A common saturated fatty acid found in fats and waxes including olive oil, palm oil, and body lipids.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The process of cleaving a chemical compound by the addition of a molecule of water.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)

cDNA cloning of 15-lipoxygenase type 2 and 12-lipoxygenases of bovine corneal epithelium. (1/375)

Bovine corneal epithelium contains arachidonate 12- and 15-lipoxygenase activity, while human corneal epithelium contains only 15-lipoxygenase activity. Our purpose was to identify the corneal 12- and 15-lipoxygenase isozymes. We used cDNA cloning to isolate the amino acid coding nucleotide sequences of two bovine lipoxygenases. The translated sequence of one lipoxygenase was 82% identical with human 15-lipoxygenase type 2 and 75% identical with mouse 8-lipoxygenase, whereas the other translated nucleotide sequence was 87% identical with human 12-lipoxygenase of the platelet type. Expression of 15-lipoxygenase type 2 and platelet type 12-lipoxygenase mRNAs were detected by Northern analysis. In addition to these two lipoxygenases, 12-lipoxygenase of leukocyte (tracheal) type was detected by polymerase chain reaction (PCR), sequencing, and Northern analysis. Finally, PCR and sequencing suggested that human corneal epithelium contains 15-lipoxygenase types 1 and 2.  (+info)

Murine 12(R)-lipoxygenase: functional expression, genomic structure and chromosomal localization. (2/375)

A cDNA, recently cloned (by Krieg et al. (1998)) from mouse skin, was shown to encode a 12(R)-lipoxygenase. When expressed in HEK cells, the recombinant protein converted methyl arachidonate into the corresponding 12-HETE ester which was shown to be the R-enantiomer by chiral phase chromatography. Neither arachidonic acid nor linoleic acid were substrates for the recombinant protein. The structure of the 12(R)-lipoxygenase gene is unique among all animal lipoxygenases in that it is divided into 15 exons and 14 introns spanning approximately 12.5 kb. By interspecific backcross analysis, the 12(R)-lipoxygenase gene was localized to the central region of mouse chromosome 11.  (+info)

Tissue-specific translational regulation of alternative rabbit 15-lipoxygenase mRNAs differing in their 3'-untranslated regions. (3/375)

By screening a rabbit reticulocyte library, an alternative 15-LOX transcript of 3.6 kb (15-LOX mRNA2) was detected containing a 1019 nt longer 3'-untranslated region (UTR2) than the main 2.6 kb mRNA (15-LOX mRNA1). In anaemic animals, northern blotting showed that 15-LOX mRNA2 was predominantly expressed in non-erythroid tissues, whereas 15-LOX mRNA1 was exclusively expressed in red blood cells and bone marrow. The 15-LOX 3'-UTR2 mRNA2 contained a novel 8-fold repetitive CU-rich motif, 23 nt in length (DICE2). This motif is related but not identical to the 10-fold repetitive differentiation control element (DICE1) of 19 nt residing in the 15-LOX UTR1 mRNA1. DICE1 was shown to interact with human hnRNP proteins E1 and K, thereby inhibiting translation. From tissues expressing the long 15-LOX mRNA2, two to three unidentified polypeptides with molecular weights of 53-55 and 90-93 kDa which bound to DICE2 were isolated by RNA affinity chromatography. A 93 kDa protein from lung cytosol, which was selected by DICE2 binding, was able to suppress translational inhibition of 15-LOX mRNA2, but not of 15-LOX mRNA1, by hnRNP E1. A possible interaction between DICE1/DICE2 cis / trans factors in translational control of 15-LOX synthesis is discussed. Furthermore, the 3'-terminal part of the highly related rabbit leukocyte-type 12-LOX gene was analysed. Very similar repetitive CU-rich elements of the type DICE1 (20 repeats) and DICE2 (nine repeats) were found in the part corresponding to the 3'-UTR of the mRNA.  (+info)

Effects of mutant p53 expression on human 15-lipoxygenase-promoter activity and murine 12/15-lipoxygenase gene expression: evidence that 15-lipoxygenase is a mutator gene. (4/375)

Human 15-lipoxygenase (h15-LO) is present on chromosome 17p13.3 in close proximity to the tumor-suppressor gene, p53. 15-LO is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. The murine BALB/c embryo fibroblast cell line, (10)1val, expresses p53 in mutant (mt) conformation when grown at 39 degrees C and in wild-type conformation when grown at 32 degrees C. Transfection of h15-LO promoter constructs (driving luciferase reporter) into (10)1val cells and into p53-deficient (10)1 cells resulted in a marked increase in h15-LO promoter activity in (10)1val cells at 39 degrees C, but not at 32 degrees C, or as compared with (10)1 cells. Transfection of h15-LO promoter deletion constructs, however, resulted in total loss of activity in both cell types at 32 degrees C and 39 degrees C. Cotransfection of (10)1 cells with h15-LO promoter (driving luciferase reporter) along with increasing levels of a mt p53 expression vector demonstrated dose-dependent capacity of mt p53 to induce 15-LO promoter activity. No effect was observed with wild-type p53. In contrast to h15-LO promoter activity, (10)1val cells had significantly lower levels of endogenous (murine) 12/15-LO (mouse analog of h15-LO) mRNA and protein when grown at 39 degrees C compared with cells grown at 32 degrees C. Our data support the hypothesis that loss of a tumor-suppressor gene (p53), or "gain-of-function activities" resulting from the expression of its mutant forms, regulates 15-LO promoter activity in man and in mouse, albeit in directionally opposite manners. The studies define a direct link between 15-LO activity and an established tumor-suppressor gene located in close chromosomal proximity.  (+info)

Albumin modifies the metabolism of hydroxyeicosatetraenoic acids via 12-lipoxygenase in human platelets. (5/375)

12-Lipoxygenase and cyclooxygenase 1 are the dominating enzymes that metabolize arachidonic acid in human platelets. In addition to the conversion of arachidonic acid to 12(S)-hydroxyeicosatetraenoic acid, 12-lipoxygenase can also utilize 5(S)-hydroxyeicosatetraenoic acid and 15(S)-hydroxyeicosatetraenoic acid to form 5(S), 12(S)-dihydroxyeicosatetraenoic acid and 14(R), 15(S)-dihydroxyeicosatetraenoic acid, respectively. Furthermore, 15(S)-hydroxyeicosatetraenoic acid works as an inhibitor for 12-lipoxygenase. In the present paper we have studied the influence of albumin on the in vitro metabolism of 5 - and 15 -hydroxyeicosatetraenoic acids, and 5,15 -dihydroxyeicosatetraenoic acid by the platelet 12-lipoxygenase. The presence of albumin reduced the formation of 5(S),12(S)- dihydroxyeicosatetraenoic acid from 5(S)-hydroxyeicosatetraenoic acid, however, it had no effect on the 12(S)-hydroxyeicosatetraenoic acid production from endogenous arachidonic acid. In contrast, when 15(S)-hydroxyeicosatetraenoic acid was incubated with activated platelets, the formation of 14(R), 15(S)- dihydroxyeicosatetraenoic acid was stimulated by the presence of albumin. Furthermore, albumin reduced the inhibitory action 15(S)-hydroxyeicosatetraenoic acid had on 12(S)-hydroxyeicosatetraenoic acid formation from endogenous arachidonic acid. However, addition of exogenous arachidonic acid (20 microm) to the incubations inverted the effects of albumin on the conversion of 15(S)-hydroxyeicosatetraenoic acid to 14(R),15(S)- dihydroxyeicosatetraenoic acid and the production of 12(S)-hydroxyeicosatetraenoic acid in these incubations. Based on the Scatchard equation, the estimates of the binding constants to albumin were 1.8 x 10(5) for 15 -HETE, 1.4 x 10(5) for 12-HETE, and 0.9 x 10(5) for 5 -HETE respectively. These results suggest an important role of albumin for the regulation of the availability of substrates for platelet 12-lipoxygenase.  (+info)

Relationship of arachidonic acid metabolizing enzyme expression in epithelial cancer cell lines to the growth effect of selective biochemical inhibitors. (6/375)

Arachidonic acid (AA) metabolizing enzymes are emerging as significant mediators of growth stimulation for epithelial cells. The relative contribution of the various family members of AA metabolizing enzymes to epithelial cancer cell growth is not known. To study this question, we first analyzed a series of epithelial cancer cells to establish the relative frequency of expression for the various enzymes. We analyzed the expression of five AA metabolizing enzymes as well as 5-lipoxygenase activating protein (FLAP) in a panel of human epithelial cancer cell lines (n = 20) using reverse transcription-PCR. From this analysis, we found that cyclooxygenase-1 (COX-1), 5-lipoxygenase (5-LOX), and FLAP were universally expressed in all cancer cell lines tested. For the remaining enzymes, the expression of COX-2, 12-LOX, and 15-LOX varied among cell lines, 60, 35, and 90%, respectively. Although the pattern of expression varied among the different cell types, all of the enzymes were expressed in all major cancer histologies. Using a panel of selective biochemical AA metabolizing enzyme inhibitors, we then evaluated the effect of these agents on cell lines with known expression status for the AA metabolizing enzymes. For the enzymes that were not universally expressed, growth inhibition by selective biochemical inhibitors did not closely correlate with the expression status of specific enzymes (P > 0.05). For the universally expressed enzymes, the LOX inhibitors were more potent growth inhibitors than the COX inhibitors. The frequent expression of the AA metabolizing enzymes suggests that AA metabolism pathway may be modulated in response to xenobiotic exposure during carcinogenesis. Although establishing a priori AA metabolizing enzyme status was not consistently informative about what AA metabolizing enzyme inhibition would be most growth inhibitory, the frequent inhibition of many epithelial cancers by these biochemical inhibitors opens a new avenue for cancer therapy and intervention in carcinogenesis.  (+info)

Resistance to type 1 diabetes induction in 12-lipoxygenase knockout mice. (7/375)

Leukocyte 12-lipoxygenase (12-LO) gene expression in pancreatic beta cells is upregulated by cytotoxic cytokines like IL-1beta. Recent studies have demonstrated that 12-LO inhibitors can prevent glutamate-induced neuronal cell death when intracellular glutathione stores are depleted. Therefore, 12-LO pathway inhibition may prevent beta-cell cytotoxicity. To evaluate the role of 12-LO gene expression in immune-mediated islet destruction, we used 12-LO knockout (12-LO KO) mice. Male homozygous 12-LO KO mice and control C57BL/6 mice received 5 consecutive daily injections of low-dose streptozotocin to induce immune-mediated diabetes. Fasting serum glucose and insulin levels were measured at 7-day intervals, and the mice were followed up for 28 days. 12-LO KO mice were highly resistant to diabetes development compared with control mice and had higher serum insulin levels on day 28. Isolated pancreatic islets were treated with IL-1beta, TNF-alpha, and IFN-gamma for 18 hours. Glucose-stimulated insulin secretion in cytokine-treated islets from C57/BL6 mice decreased 54% from that of untreated islets. In marked contrast, the same cytokine mix led to only a 26% decrease in islets from 12-LO KO mice. Furthermore, cytokine-induced 12-hydroxyeicosatetraenoic acid (12-HETE) production was absent in 12-LO KO islets but present in C57/BL6 islets. Isolated peritoneal macrophages were stimulated for 48 hours with IFN-gamma + LPS and compared for nitrate/nitrite generation. 12-LO KO macrophages generated 50% less nitrate/nitrite when compared with C57BL/6 macrophages. In summary, elimination of leukocyte 12-LO in mice ameliorates low dose streptozotocin-induced diabetes by increasing islet resistance to cytokines and decreasing macrophage production of nitric oxide.  (+info)

Evidence for 12-lipoxygenase induction in the vessel wall following balloon injury. (8/375)

OBJECTIVE: Vascular smooth muscle cell (VSMC) migration and proliferation are key events in the development of atherosclerosis and restenosis following angioplasty. These events are mediated by several growth factors and cytokines whose cellular effects include activation of phospholipases and arachidonic acid metabolism via the lipoxygenase (LO) pathway. Since 12-LO products have potent growth and chemotactic effects, we have examined if 12-LO is upregulated in the neointima of injured rat carotid arteries and also if LO inhibition could attenuate neointimal thickening. METHODS: The left common carotid arteries of male Sprague Dawley rats were injured using a 1.8 F PTCA balloon catheter. Four-fourteen days after injury, injured and uninjured tissue samples were processed for histology, and immunohistochemistry or polymerase chain reaction (PCR) to examine 12-LO expression. RESULTS: Twelve days after injury, immunohistochemical staining with a 12-LO antibody revealed intense staining in injured left carotid arteries, mainly in neointimal VSMCs and inflammatory cells, but not in the uninjured right arteries. There was also a marked upregulation of 12-LO mRNA (over five-fold by competitive PCR) in the injured arteries. Treatment of the arteries with a LO inhibitor, phenidone, soon after injury resulted in significant inhibition of neointimal thickening. In contrast, a cyclooxygenase inhibitor, ibuprofen, had no effect. CONCLUSIONS: These results indicate for the first time that balloon injury results in marked induction of 12-LO mRNA and protein expression in the vessel wall. Furthermore, LO pathway activation may mediate, at least in part, the development of the lesion or plaque instability, suggesting a novel target for therapeutic intervention to block these pathological events.  (+info)

TY - JOUR. T1 - Role of epidermis-type lipoxygenases for skin barrier function and adipocyte differentiation. AU - Fürstenberger, Gerhard. AU - Epp, Nikolas. AU - Eckl, Katja Martina. AU - Hennies, Hans Christian. AU - Jørgensen, Claus. AU - Hallenborg, Philip. AU - Kristiansen, Karsten. AU - Krieg, Peter. PY - 2007/1/1. Y1 - 2007/1/1. N2 - 12R-lipoxygenase (12R-LOX) and epidermis-type LOX-3 (eLOX-3) are novel members of the multigene family of mammalian LOX. A considerable gap exists between the identification of these enzymes and their biologic function. Here, we present evidence that 12R-LOX and eLOX-3, acting in sequence, and eLOX-3 in combination with another, not yet identified LOX are critically involved in terminal differentiation of keratinocytes and adipocytes, respectively. Mutational inactivation of 12R-LOX and/or eLOX-3 has been found to be associated with development of an inherited ichthyosiform skin disorder in humans and genetic ablation of 12R-LOX causes a severe impairment ...
Two types of 12-lipoxygenase that catalyze the transformation of arachidonic acid to 12(S)-hydroperoxyeicosatetraenoic acid (12-HPETE) have been previously classified into platelet-type and leukocyte-type categories. Here, we document, for the first time, a molecular characterization of both forms within the same species. The amino acid sequence of the murine platelet 12-lipoxygenase deduced from its cDNA is 58% identical to the murine spleen/leukocyte 12-lipoxygenase. Expression constructs carrying the cDNAs for the two 12-lipoxygenase forms were introduced into human embryonic kidney 293 cells. The platelet-type enzyme metabolized arachidonic acid exclusively to 12-HPETE, whereas the leukocyte-type enzyme formed both 12-HPETE and 15-hydro(pero)xyeicosatetraenoic acid in a ratio of approximately 3:1. Linoleic acid was metabolized to a similar extent by the latter enzyme to 13-hydro(pero)xyoctadecadienoic acid but not by the platelet enzyme. Mutagenesis and deletion of the highly conserved ...
Convincing evidence exists implicating leukocyte-type 12/15-LO as a key player in the development of atherosclerosis and restenosis. KO of the 12/15-LO gene in apolipoprotein E- and LDL receptor-deficient mice, 2 models of robust atherosclerosis, resulted in a marked decrease in atherosclerosis development in vivo.5,7 Marked upregulation of 12/15-LO occurred in VSMCs after balloon carotid injury in rats, and restenosis was significantly blocked with a pharmacological 12/15-LO inhibitor as well as a specific ribozyme targeted to cleave 12/15-LO.4,27 Many events and pathways involving 12/15-LO have been proposed to contribute to atherosclerosis and restenosis. Much is understood concerning the ability of 12/15-LO within macrophages to participate in the oxidation of LDL, an early event in vascular lesion formation.2,7-9,28 However, 12/15-LO activity and products also increase in endothelial cells and VSMCs at the time of injury and in response to growth factors and cytokines. 12S-HETE, the ...
A LOX definition according to enzyme classification is linoleate: oxygen oxidoreductase (for plant LOX) and arachidonate: oxygen oxidoreductase (for mammalian LOX). Historically LOX are classified according to their positional specificity of the dioxygenation of their most common substrates linoleate (C-18) in plants, and arachidonic acid (C-20) in mammals. Accordingly, a LOX reacting at the ω-6 position of the substrate (as counted from the tail end of the fatty acid) in plants is referred to as a 13-LOX (alternate: linoleate ω6-LOX) and in mammals as a 15-LOX (alternate: arachidonate ω6-LOX). In plants 9-, and 13-LOX and in mammals, 5-, 8-, 12- and 15-LOX are known. When necessary, the stereoconfiguration is specified (e.g. 12S-LOX and 12R-LOX). In mammals, isoenzymes that exhibit the same position-specificity are named additionally after the prototypical tissue of their occurrence (e.g. platelet-type, leukocyte-type and epidermis-type 12S-LOX) [1 ...
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21:30, 9 February 2014 (diff , hist) . . (+18)‎ . . m Eliezer Yudkowsky ‎ (Changed link which previously redirected back to this page to a more useful link to EYs LW posts. Simplified wikicode in a link.) ...
21:30, 9 February 2014 (diff , hist) . . (+18)‎ . . m Eliezer Yudkowsky ‎ (Changed link which previously redirected back to this page to a more useful link to EYs LW posts. Simplified wikicode in a link.) ...
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Complete information for ALOX15 gene (Protein Coding), Arachidonate 15-Lipoxygenase, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
jo to existuje, t eba klackem po hlav . um te si p edstavit e z jedn ko ky m ete m t za rok p ibli n 15 kus potomk ..... a z toho je t eba 10 ko ek a 5 kocour .. ili 11 ko ek kr t 15 potomk za rok je 165. za dva roky z jedn ko ky je mo n aby kolem bar ku l talo 181 ko ek. d ky nechci. ...
Telef nica O2 inovovala svou voln p stupnou SMS br nu 1188. M ete z n pos lat a 100 SMS za hodinu zdarma, a to v pln m rozsahu 160 znak . K vyu it t to nab dky je ale nutn registrace doprov zen mohutn m dotazn kem.
Prihláste sa do newslettra a získajte pravidelné informácie o super ponukách, akciách a zľavách. Zo zasielania sa môžete kedykoľvek odhlásiť.. Prihláste sa na odber noviniek. ...
Stran se povezuje s katalogi knjižnic, knjigarn in drugih virov knjig, kjer lahko iščete knjigo z uporabo ISBN-ja. Če ste prispeli na to stran s klikom na povezavo številke ISBN z Wikipedijine strani, potem povezave spodaj (označene s »Poišči knjigo«) iščejo določeno knjigo po tej številki ISBN. Če želite spremeniti iskalne povezave spodaj, vnesite drug ISBN v iskalno polje ISBN. Presledki in pomišljaji v številu ISBN niso pomembni. Opozorilo: Število se začne izza dvopičja ISBN-10: in ISBN-13:. ...
Stran se povezuje s katalogi knjižnic, knjigarn in drugih virov knjig, kjer lahko iščete knjigo z uporabo ISBN-ja. Če ste prispeli na to stran s klikom na povezavo številke ISBN z Wikipedijine strani, potem povezave spodaj (označene s »Poišči knjigo«) iščejo določeno knjigo po tej številki ISBN. Če želite spremeniti iskalne povezave spodaj, vnesite drug ISBN v iskalno polje ISBN. Presledki in pomišljaji v številu ISBN niso pomembni. Opozorilo: Število se začne izza dvopičja ISBN-10: in ISBN-13:. ...
References for Abcams Recombinant Human 5 Lipoxygenase protein (ab114310). Please let us know if you have used this product in your publication
Ete was a five-year-old boy. At 2:00 in the morning, he woke up screaming with pain from a headache. Mom gave him Tylenol but he was not able to go back to sleep. By the time I saw him, he had suffered with headache for almost twenty-four hours. His vital signs were normal and he had no other complaints or recent illnesses, but Ete looked miserable, holding the top of his head with tears in his eyes.. In adults, sudden on-set headaches like this may be caused by a subarachnoid hemorrhage. This is a very serious condition caused by the rupture of an abnormal blood vessel, usually an aneurysm, at the base of the brain. If the aneurysm is not discovered and treated, complications can lead to brain damage or death.. The work-up for adult patients with this kind of headache is very straightforward. A CT scan of the head shows brain hemorrhage in about ninety percent of people who have one. If the CT is normal, we recommend a lumbar puncture to make sure there is no blood in the fluid that bathes the ...
sp:LIDS_GAEGR] linoleate 8R-lipoxygenase / 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase (EC:; K17864 linoleate 8R-lipoxygenase / 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase [EC:] ...
sp:LIDS_GAEGR] linoleate 8R-lipoxygenase / 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase (EC:; K17864 linoleate 8R-lipoxygenase / 9,12-octadecadienoate 8-hydroperoxide 8S-isomerase [EC:] ...
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Article UV systems for spas & hydro pools - Case Study. With the rise of health centres and leisure clubs throughout the UK, a key feature in many modern facilities is the inclusion of spas and hydro pools. As a specialist aquatic application, spas a...
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Deschamps, J. D., Gautschi, J. T., Whitman, S., Johnson, T. A., Gassner, N. C., Crews, P., & Holman, T. R. (2007). Discovery of platelet-type 12-human lipoxygenase selective inhibitors by high-throughput screening of structurally diverse libraries. Bioorg ...
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Lahka negovalna in zaščitna krema za maksimalno, dolgo trajajočo navlaženost. Obogatena je z izredno učinkovitim hydro complex, ki nudi takojšnjo ...
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Za n m ch pat, kde je j dro pudla.Moje plej da dotaz postr d logickou koherenci proto, e jsem myln p edpokl dala, e n kter ch z t ch v c v te. Pokud se k v m ani nedoneslo, e Gallo v sledky laboratorn ch m en zm nil, pak ch pu, e se k tomu nem ete vyj d it. Naposledy v m zde d v m odkaz na cel zn n otev en ho dopisu asopisu Science od des tek odborn k pot , co to vy lo najevo. . V n m si m ete zhruba p e st, o co lo. Dokonce jsou k dispozici i kopie zpr v z laborato e psan na stroji, do kter ch pozd ji Gallo rukou ud lal zm ny - prakticky co se mu nehodilo za krtal a napsal opak. Jestli e se t m odm t te zab vat, pak nem te pr vo m napadat. J netvrd m e je to pravda, pt m se, jak se k tomu stav odborn ve ejnost. Pokud ta to ignoruje, pak nem e po m cht t, abych konkretizovala sv dotazy - to bych mohla do alel ja.. ...
1J&]Th~~ ~~~~~ 3AKA)I(III Ill nOJlY~III! BPEMSI nPOCTO 5b1Tb 3AOPOBbiM! 3n1 ,KI.13HeHHO none3Hble KHI.1rl.1 noMoryr saM nony- ~1.1Tb AOCTaTO~Hble 3HaHJ.1SI, ~T06bl cnpaBJ.1TbCSI C mo6biMI.1 HeAyraMII1, nopTstll.\111MI.1 eawy ,KI.13Hb 1.1 ,KI!13Hb BaWJ.1X 6nii13KJ.1X. ~TO COBETYIOT BPA~I!1 3HI.\111KflOneA1115l caMblX pacnpoCTpaHeHHbiX 3a6onesaH111~ . npoCTO 111 AOCTynHO- 0 caxapHOM A111a6eTe, OCTeOXOHAP03e , r111nepTOH111111 , ap111TM111111 , naHKpeaT111Te, npocTanne , 6poHx111an b HO~ acTMe , AepMaT111Te . CospeMeHHble MeTOAbl o6cneAOBaH1115l 111 n e4eH1115l , HOB ble 111 HaAe,KHble cnoco6bl 03AOposneH1115l . KAKnE~ii!Tb?n03BOHO~HHK HD3BOHOMHUK 0 0 0 0 60n111 B Cn111He , OrpaH1114eHHa5l nOAB111,KHOCTb n05lCH1111.\bl, OHeMeH111e KOH e4HOCTe~, 4aCTble ronoBHble 6on111 HepeAKO Ha4111HaJOT 6ecnoK0111Tb y,Ke B Mono AOM B03pacTe . Bbl Y3HaeTe 0 np1114111HaX 111 npocp111naKT111Ke OCTeOXOHAP03a ; 0 ne4eH111111 Me,Kn03BOHKOBO~ rp bi,K111111 PaA111Kyn111Ta; o cnoco6ax npeoAoneH1115l nocneACTB111~ ...
Oran ov postele 80x200 cm. Ob ben postele 80x200 cm prvotriednej kvality u n s n jdete u od 60 . i u h ad te postele 80x200 cm v modernom alebo klasickom t le, u n s si m ete vybra a z 170 druhov. V ponuke m me man elsk i jednol kov postele, rozkladacie i s lo n m priestorom a na deti akaj i pekn poschodov postele. V etky si m ete jednoducho vyh ada pomocou n ho praktick ho filtra. Doru me v m ich a domov. Nev hajte sa na n s obr ti . Ta prav poste 80x200 cm ak pr ve na v s.
Ak zaznamenávate cvičenie, hodnoty dennej srdcovej frekvencie odrážajú zvýšenú srdcovú frekvenciu a spotrebu kalórií z tréningu. Majte však na pamäti, že graf a údaje o spotrebe sú priemery. Ak sú špičkové hodnoty vašej srdcovej frekvencie počas cvičenia na úrovni 200 úderov za minútu, graf nezobrazuje túto maximálnu hodnotu, ale priemer za 24 minút, počas ktorých ste dosiahli najvyššiu hodnotu.. Displej dennej srdcovej frekvencie je možné zobraziť až po aktivovaní funkcie dennej srdcovej frekvencie. Túto funkciu môžete zapnúť alebo vypnúť v nastaveniach v položke Activity. Ak ste na displeji dennej srdcovej frekvencie, nastavenia aktivity môžete zobraziť aj podržaním stlačeného stredného tlačidla.. Keď je zapnutá funkcia dennej srdcovej frekvencie, hodinky pravidelne aktivujú optický snímač srdcovej frekvencie na kontrolu vašej srdcovej frekvencie. Mierne sa tým zvyšuje spotreba batérie.. ...
Ima puno videa o Access Barsu koje možete istražiti! Evo nekoliko sa kojima predlažemo da započnete, bez određenog redoslijeda. Što vam govori i golica vašu znatiželju? Možete također nastaviti na Youtube kanalu Access Consciousnessa. Ili saznati o Accessovom prijenosu uživo za još više mogućnosti. ...
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The Amino Whey je naš najpopularniji visokopostotni protein sa 86% čistog proteina. Ima dodatne amino kiseline, manje od 1g OH i masti i različite ukuse.
mám v plánu dietu (něco jako kdybych šel na prkna s tím, že nejspíš nepůjdu), prostě pořádně to sesekat, ať konečně vypadám jako sportující člověk. Mám dotaz ohledně před a potréninkového napoje. Bohužel nemám prachy na to, abych si koupil amina + bcaa + protein/hydro, proto se ptám jestli si vystačím jen s hydrem (alespoň v prní půlce diety, kdy jsou S konstantní, vyhodí se kraviny z jídelníčku a přidá se pohyb) a jestli mám vzít jen hydro nebo např. Ultra Speed 80 Fair Power ®, co je podle Vás lepší ...
This is a brilliant facial which goes deep and works from the inside out. There is no down time but some clients may feel a redness which can be covered with make up should you choose. You will start to notice results very quickly and this is a great boost to the skin. You can have this treatment once a month. Also, great for neck and décolletage.. ...
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Ever since the Kratky Hydro Show on Tuesday I have been thinking about hydro in new ways. One of the biggest is for plant propagation and there is a ton more.
Poate fi pansament primar pe situri donoare/receptoare de piele, escare de st. I, II, pansament pentru dermatite chimice perifistulare, ulcere de gamba de etiologie venoasa, leziuni de psoriazis in asociere cu Fluocinolon, abraziuni, arsuri de gradul I, II, ulcere de gamba de etiologie arteriala, zone expuse frictiunilor si aparitiei leziunilor de decubit etc ...
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Exhaling, bend in the waist and touch the forehead on the floor keeping the hands straight. After taking the final position, continue smooth breathing.(as shown in the picture). Position Like Yogamudra here too the abdomen gets folded. Further, attempt should be made to have this fold as compl. ete as possible. The frontward stretched arms greatly help in getting this fold. However, the hands should be kept as much outstretched as possible ...
"Entrez Gene: ALOX12B arachidonate 12-lipoxygenase, 12R type". Boeglin WE, Kim RB, Brash AR (June 1998). "A 12R-lipoxygenase in ... Among the human lipoxygenases, ALOX12B is most closely (54% identity) related in amino acid sequence to ALOXE3 ALOX12B ... Arachidonate 12-lipoxygenase, 12R type, also known as ALOX12B, 12R-LOX, and arachiconate lipoygenase 3, is a lipoxygenase-type ... The gene is located on chromosome 17 at position 13.1 where it forms a cluster with two other lipoxygenases, ALOXE3 and ALOX15B ...
They include (HEPE is hydroxy-eicsapentaenoic acid): 5-HEPE (see Arachidonate 5-lipoxygenase#Eicosapentaenoic acid), 12-HEPE, ... The enzymes 15-lipoxygenase-1 (15-LO-1 or ALOX15) and 15-lipoxygenase-2 (15-LO-2, ALOX15B) metabolize arachidonic acid to the S ... Other reactions of lipoxygenases generate cellular damage; murine models implicate 15-lipoxygenase in the pathogenesis of ... Lipoxygenases (LOXs): 5-Lipoxygenase (5-LOX or ALOX5) initiates the metabolism of arachidonic acid to 5- ...
Nugteren, D. H. (1975). "Arachidonate lipoxygenase in blood platelets". Biochimica et Biophysica Acta (BBA) - Lipids and Lipid ... Arachidonate 15-lipoxygenase-1 (15-LO-1, 15-LOX-1, ALOX15), which is expressed in far more tissues that ALOX12, metabolizes ... sequence identity to mouse Arachidonate 8-lipoxygenase. Mouse e-12LO metabolizes arachidonic acid predominantly to 12(S)-HETE ... "Arachidonate dilates basilar artery by lipoxygenase-dependent mechanism and activation of K(+) channels". American Journal of ...
Arachidonate lipoxygenase 3. Arachidonate 12-lipoxygenase, 12R type Epidermolytic hyperkeratosis (bullous ichthyosis, bCIE) ...
... may refer to: Arachidonate 5-lipoxygenase, an enzyme Arachidonate 12-lipoxygenase, an enzyme This set ...
... lipoxygenase MeSH D12.776.556.579.374.450.025 - arachidonate lipoxygenases MeSH D12.776.556.579.374.450.025.020 - arachidonate ... Type XII collagen MeSH D12.776.860.300.250.600 - procollagen MeSH D12.776.860.300.250.700 - tropocollagen MeSH D12.776.860.300. ... arachidonate 15-lipoxygenase MeSH D12.776.556.579.374.687 - retinal dehydrogenase MeSH D12.776.556.579.374.925 - tyrosine 3- ... 5-lipoxygenase MeSH D12.776.556.579.374.450.025.025 - arachidonate 12-lipoxygenase MeSH D12.776.556.579.374.450.025.030 - ...
... lipoxygenase MeSH D08.811.682.690.416.444.050 - arachidonate lipoxygenases MeSH D08.811.682.690.416.444.050.055 - arachidonate ... arachidonate 15-lipoxygenase MeSH D08.811.682.690.416.444.525 - protocatechuate 3,4-dioxygenase MeSH D08.811.682.690.416.722 - ... 5-lipoxygenase MeSH D08.811.682.690.416.444.050.060 - arachidonate 12-lipoxygenase MeSH D08.811.682.690.416.444.050.065 - ... steroid 12-alpha-hydroxylase MeSH D08.811.682.690.708.170.915.737 - steroid 16-alpha-hydroxylase MeSH D08.811.682.690.708.170. ...
Nugteren DH (February 1975). "Arachidonate lipoxygenase in blood platelets". Biochimica et Biophysica Acta (BBA) - Lipids and ... Delta12-lipoxygenase, 12Delta-lipoxygenase, and C-12 lipoxygenase. ALOX12, often termed plate platelet-type 12-lipoxygenase, is ... "Entrez Gene: ALOX12 arachidonate 12-lipoxygenase". Yamamoto S, Suzuki H, Ueda N (March 1997). "Arachidonate 12-lipoxygenases". ... Muñoz-Garcia A, Thomas CP, Keeney DS, Zheng Y, Brash AR (March 2014). "The importance of the lipoxygenase-hepoxilin pathway in ...
Arachidonate 15-lipoxygenase type II (ALOX15B), also termed 15-lipoxygenase-2, 15-LOX-2, and 15-LOX-2. It metabolizes ... soybean lipoxygenase L1 and L3, coral 8-lipoxygenase, human 5-lipoxygenase, rabbit 15-lipoxygenase and porcine leukocyte 12- ... erythrocyte type 15-lipoxygenase (or 15-lipoxygenase, erythrocyte type), reticulocyte type 15-lipoxygenase (or 15-lipoxygenase ... "ALOX5 arachidonate 5-lipoxygenase [Homo sapiens (human)] - Gene - NCBI". Haeggström, J. Z.; Funk, C. D. (2011). "Lipoxygenase ...
... , also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13 ... Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. It transforms essential fatty acids (EFA) ... "Alox5 - arachidonate 5-lipoxygenase". WikiGenes. Fahel JS, de Souza MB, Gomes MT, Corsetti PP, Carvalho NB, Marinho FA, de ... Arachidonate+5-Lipoxygenase at the US National Library of Medicine Medical Subject Headings (MeSH) Human ALOX5 genome location ...
... s are compounds that slow or stop the action of the arachidonate 5-lipoxygenase (5- ... Antileukotriene agents Arachidonate 5-lipoxygenase ALOX5-inhibiting drugs David L. Nelson, Michael M. Cox. Lehninger's ... 39: 1302-4. PMID 12018529.CS1 maint: multiple names: authors list (link) 1. Arachidonate 5-lipoxygenase ...Specific function: ... Zileuton is a specific inhibitor of 5-lipoxygenase and thus inhibits leukotriene (LTB4, LTC4, LTD4, and LTE4) formation. Both ...
"Depletion of phospholipid hydroperoxide glutathione peroxidase up-regulates arachidonate metabolism by 12S-lipoxygenase and ... untranslated region of human glutathione peroxidase 4 influences lipoxygenase metabolism". Blood Cells Mol. Dis. 29 (2): 174-8 ... 16 (12): 1180-1191. doi:10.1038/ncb3064. PMC 4894846. PMID 25402683. Smith AC, Mears AJ, Bunker R, Ahmed A, MacKenzie M, ... "Glutathione peroxidase 4 senses and translates oxidative stress into 12/15-lipoxygenase dependent- and AIF-mediated cell death ...
... arachidonate 15-lipoxygenase 2, 15-lipoxygenase-2, 15-LOX-2, 15-LO-2, arachidonate 15-lipoxygenase type II, arachidonate 15- ... ALOX15 (also termed arachidonate 15-lipoxygenase, 15-lipoxygenase-1, 15-LO-1, 15-LOX-1) is, like other lipoxygenases, a seminal ... Consequently, human ALOX15 is now referred to as arachidonate-15-lipoxygenase-1, 15-lipoxygenase-1, 15-LOX-1, 15-LO-1, human 12 ... Sigal E, Grunberger D, Craik CS, Caughey GH, Nadel JA (April 1988). "Arachidonate 15-lipoxygenase (omega-6 lipoxygenase) from ...
Arachidonate 8-lipoxygenase. *Arachidonate 15-lipoxygenase/ALOX15. *Linoleate 11-lipoxygenase. *other dioxygenase: Catechol ... Retrieved May 12, 2014.. *^ SW, Ryter; J, Alam (April 2006). "Heme oxygenase-1/carbon monoxide: from basic science to ...
Arachidonate 8-lipoxygenase. *Arachidonate 15-lipoxygenase/ALOX15. *Linoleate 11-lipoxygenase. *other dioxygenase: Catechol ...
Antileukotriene Arachidonate 5-lipoxygenase inhibitor Antihistamines Hooper, Nigel M. (2013). Membrane dipeptidase. Handbook of ... Leukotrienes are produced from arachidonic acid by 5-lipoxygenase (which is made from phospholipids in the cell membrane) and ... ISBN 978-0-12-382219-2. "Montelukast". Medicines Complete. doi:10.18578/BNF.236887386. Retrieved October 9, 2019. "Montelukast ...
Ghosh J, Myers CE (1998). "Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells ... mediates the survival-promoting effects of arachidonate 5-lipoxygenase in prostate cancer cells". Cancer Lett. 336 (1): 185-95 ... Hussey HJ, Tisdale MJ (1996). "Inhibition of tumour growth by lipoxygenase inhibitors". Br. J. Cancer. 74 (5): 683-687. doi: ... Wijkander J, O'Flaherty JT, Nixon AB, Wykle RL (1995). "5-Lipoxygenase products modulate the activity of the 85-kDa ...
Arachidonate 5-lipoxygenase ...Specific function: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a ... arachidonate 5-lipoxygenase) or leukotriene receptor antagonist (cysteinyl leukotriene receptors) and consequently opposes the ... drugs such as MK-886 that block the 5-lipoxygenase activating protein (FLAP) inhibit functioning of 5-lipoxygenase and may help ... Zileuton is a specific inhibitor of 5-lipoxygenase and thus inhibits leukotriene (LTB4, LTC4, LTD4, and LTE4) formation. Both ...
Arachidonate 15-lipoxygenase type II is an enzyme that in humans is encoded by the ALOX15B gene. ALOX15B, also known as 15- ... "Entrez Gene: ALOX15B arachidonate 15-lipoxygenase, type B". Human ALOX15B genome location and ALOX15B gene details page in the ... Arachidonate 15-lipoxygenase 15-hydroxyicosatetraenoic acid ALOX15 GRCh38: Ensembl release 89: ENSG00000179593 - Ensembl, May ... "Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells". The ...
First, human eosinophils use Arachidonate 15-lipoxygenase-1 (or possibly Arachidonate 15-lipoxygenase-2 to metabolize 5-oxo-ETE ... oxygenation of this arachidonic acid by activated arachidonate 5-lipoxygenase (ALOX5) to form 5(S)-hydroperoxy-6E,8Z,11Z,14Z- ... This transcellular production typically involves the limited variety of cell types that express active 5-lipoxygenase, lack ... and lipoxygenase-derived eicosanoids". Drug Metabolism and Disposition. 39 (2): 180-90. doi:10.1124/dmd.110.035121. PMC 3033693 ...
Arachidonate 5-lipoxygenase-activating protein also known as 5-lipoxygenase activating protein, or FLAP, is a protein that in ... Zintzaras E, Rodopoulou P, Sakellaridis N (March 2009). "Variants of the arachidonate 5-lipoxygenase-activating protein ( ... "Associations of genetic polymorphisms of arachidonate 5-lipoxygenase-activating protein with risk of coronary artery disease in ... "Cytogenetic and radiation hybrid mapping of human arachidonate 5-lipoxygenase-activating protein (ALOX5AP) to chromosome 13q12 ...
Kuhn, Hartmut; Walther, Matthias; Kuban, Ralf Jürgen (2002). "Mammalian arachidonate 15-lipoxygenases". Prostaglandins & Other ... 15-Lipoxygenase 1[edit]. 15-lipoxygenase 1 (ALOX15), while best known for converting the 20 carbon polyunsaturated fatty acid, ... 15-lipoxygenase 2[edit]. 15-lipoxygenase type 2 (ALOX15B) strongly prefers arachidonic acid over linoleic acid and in ... and 15-lipoxygenase-2, and selected metabolites of the latter lipoxygenases show no such association.[66][67][68] Similarly ...
Arachidonate 5-lipoxygenase Arachidonate 15-lipoxygenase Leukotriene 15-Hydroxyicosatetraenoic acid Greene ER, Huang S, Serhan ... The primary product of the lipoxygenase, 15-HPETE is believed to react with the enzyme further to produce the 14,15-epoxide, ... Claesson HE (September 2009). "On the biosynthesis and biological role of eoxins and 15-lipoxygenase-1 in airway inflammation ... Forsell PK, Brunnström A, Johannesson M, Claesson HE (2012). "Metabolism of anandamide into eoxamides by 15-lipoxygenase-1 and ...
"Entrez Gene: ALOXE3 arachidonate lipoxygenase 3". Schneider C, Brash AR (August 2002). "Lipoxygenase-catalyzed formation of R- ... Epidermis-type lipoxygenase 3 (ALOXE3 or eLOX3) is a member of the lipoxygenase family of enzymes; in humans, it is encoded by ... The epidermis-type lipoxygenases are now regarded as a distinct subclass within the multigene family of mammalian lipoxygenases ... Among the human lipoxygenases, ALOXE3 is most closely (54% identity) related in amino acid sequence to ALOX12B. ALOXE3, ALOX12B ...
Kuhn, Hartmut; Walther, Matthias; Kuban, Ralf Jürgen (2002). "Mammalian arachidonate 15-lipoxygenases". Prostaglandins & Other ... and 15-lipoxygenase-2, and selected metabolites of the latter lipoxygenases show no such association. Similarly selective ... "Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid ... 15-lipoxygenase type 2 (ALOX15B) strongly prefers arachidonic acid over linoleic acid and in consequence is relatively poor in ...
Arachidonate 5-Lipoxygenase (processes essential fatty acids to leukotrienes, which are important agents in the inflammatory ... 2013-12-24. Retrieved 2017-03-04. "Search results - 10[CHR] AND "Homo sapiens"[Organism] AND ("has ccds"[Properties] AND alive[ ... 2017-05-12. Retrieved 2017-05-19. "Chromosome 10: Chromosome summary - Homo sapiens". Ensembl Release 88. 2017-03-29. Retrieved ... 90 (1-2): 1-12. doi:10.1159/000015653. PMID 11060438. S2CID 28931509. Gilbert F (2001). "Chromosome 10". Genet Test. 5 (1): 69- ...
Arachidonate 5-lipoxygenase has been identified as playing a significant role in the survival of prostate cancer cells.[186][ ... arachidonate 5-lipoxygenase inhibitors produce massive, rapid programmed cell death in prostate cancer cells.[186][187][188] ... "Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells". Proc. Natl. Acad. Sci. U. ... The 5-lipoxygenase (5-LOX) pathway is implicated in the development and progression of human cancers. 5-LOX, whose crystal ...
... , also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13 ... that in humans is encoded by the ALOX5 gene.[5] Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. ... "Alox5 - arachidonate 5-lipoxygenase". WikiGenes.. *^ Fahel JS, de Souza MB, Gomes MT, Corsetti PP, Carvalho NB, Marinho FA, de ... arachidonate 5-lipoxygenase activity. • dioxygenase activity. • metal ion binding. • protein binding. • oxidoreductase activity ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... InChI=1S/C17H14Cl2F2N2O3/c18-11-6-22-7-12(19)15(11)23-16(24)10-3-4-13(26-17(20)21)14(5-10)25-8-9-1-2-9/h3-7,9,17H,1-2,8H2,(H,22 ...
When acting on GLA, arachidonate 5-lipoxygenase produces no leukotrienes and the conversion by the enzyme of arachidonic acid ... Archived from the original (PDF) on 2014-01-12.. *^ Nasaruddin, Mohd hanif; Noor, Noor Qhairul Izzreen Mohd; Mamat, Hasmadi ( ... InChI=1S/C18H30O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18(19)20/h6-7,9-10,12-13H,2-5,8,11,14-17H2,1H3,(H,19,20)/b7-6-,10- ... InChI=1/C18H30O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18(19)20/h6-7,9-10,12-13H,2-5,8,11,14-17H2,1H3,(H,19,20)/b7-6-,10-9 ...
5-Lipoxygenase activating protein/Arachidonate 5-lipoxygenase. *LTA4 hydrolase (B4 synthesis). *LTC4 synthase ... 12 q22. Leukotriene A4 hydrolase, also known as LTA4H is a human gene.[1][2][3] The protein encoded by this gene is a ...
Bryant, R.W., Bailey, J.M., Schewqe, T. and Rapoport, S.M. (1982). „Positional specificity of a reticulocyte lipoxygenase. ... B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7,, 3.4.21/22/23/24, ... Oliw, E.H. & Sprecher, H. (1989). „Metabolism of polyunsaturated fatty acids by an (n-6)-lipoxygenase associated with human ... Narumiya, S. & Salmon, J.A. (1982). „Arachidonic acid-15-lipoxygenase from rabbit peritoneal polymorphonuclear leukocytes". ...
Brash A. R., Boeglin W. E., Chang M. S. (Jun 1997)։ «Discovery of a second 15S-lipoxygenase in humans»։ Proc Natl Acad Sci U S ... 5,8,11,14-all-cis-Eicosatetraenoic acid; all-cis-5,8,11,14-Eicosatetraenoic acid; Arachidonate. ... Zhu D, Ran Y (May 2012)։ «Role of 15-lipoxygenase/15-hydroxyeicosatetraenoic acid in hypoxia-induced pulmonary hypertension»։ J ... Eoxins are proinflammatory arachidonic acid metabolites produced via the 15-lipoxygenase-1 pathway in human eosinophils and ...
Other lipoxygenases-8-LO, 12-LO and 15-LO-make other eicosanoid-like products. To act, 5-LO uses the nuclear-membrane enzyme 5- ... Phinney, SD; RS Odin; SB Johnson & RT Holman (March 1, 1990). "Reduced arachidonate in serum phospholipids and cholesteryl ... Lipoxygenase oxidation removes no C=C double bonds, and leads to the LK. After oxidation, the eicosanoids are further modified ... DGLA and EPA compete with AA for access to the cyclooxygenase and lipoxygenase enzymes. So the presence of DGLA and EPA in ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... InChI=1S/C22H30N2O2/c1-26-22(20-10-6-3-7-11-20)18-24-16-14-23(15-17-24)13-12-21(25)19-8-4-2-5-9-19/h2-11,21-22,25H,12-18H2,1H3 ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... up to 12 h) compared to short-acting β2 agonists such as salbutamol (albuterol), which are effective for 4 h to 6 h. LABAs such ... 12-13,19-20,23-24H,9,11H2,1-2H3,(H,21,22)/t13-,19+/m1/s1 Y ...
... arachidonate 5-lipoxygenase) or leukotriene receptor antagonists (cysteinyl leukotriene receptors) and consequently oppose the ... 34 (12): 3315-3325. doi:10.1002/eji.200425461. PMID 15549777.. *^ Mathison, Ronald D.; Davison, Joseph S. (2006). "The ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... Theophylline is excreted unchanged in the urine (up to 10%). Clearance of the drug is increased in children (age 1 to 12), ... InChI=1S/C7H8N4O2/c1-10-5-4(8-3-9-5)6(12)11(2)7(10)13/h3H,1-2H3,(H,8,9) Y ... 12 hours for those with congestive heart failure NYHA class I-II, 24 hours for those with congestive heart failure NYHA class ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... 2-[(1S, 2S, 4R, 8S, 9S,11S, 12S, 13R)-6-cyclohexyl-11-hydroxy-9, 13-dimethyl-16-oxo-5, 7-dioxapentacyclo [,9.04, 8.013 ... It was patented in 1990 and approved for medical use in 2005.[3] The drug was approved for adults and children 12 and over by ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... doi:10.7326/0003-4819-144-12-200606200-00126. PMID 16754916.. *^ "US fluticasone propionate/Salmeterol label" (PDF). FDA. April ... On January 30, 2019, the FDA granted Mylan N.V. the first generic approval for Advair Diskus.[12] ...
If arachidonate is acted upon by a lipoxygenase instead of cyclooxygenase, Hydroxyeicosatetraenoic acids and leukotrienes are ... The prostaglandins are synthesized in the cell membrane by the cleavage of arachidonate from the phospholipids that make up the ... The arachidonate is then acted upon by the cyclooxygenase component of prostaglandin synthase. This forms a cyclopentane ring ... 12. Print. "MetaCyc pathway: superpathway of fatty acids biosynthesis (E. coli)". "Fatty Acids: Straight-chain Saturated, ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... 300 (12): 1439-50. doi:10.1001/jama.300.12.1439. PMID 18812535.. *^ FDA. Follow-Up to the October 2008 Updated Early ... 12)24-17)23-18(21)19(22,14-5-3-7-25-14)15-6-4-8-26-15/h3-8,11-13,16-17,22H,9-10H2,1-2H3/q+1/t11?,12-,13+,16-,17-/m0/s1 Y ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... These medications may take longer to begin working, but relieve airway constriction for up to 12 hours. Commonly taken twice a ...
Arachidonate 5-lipoxygenase. *Atrophin 1. *BH3 interacting-domain death agonist. *BRAF (gene) ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... Retrieved 12 February 2013.. *^ a b Bailey J, McCarthy C, Smith RF (October 2011). "Clinical inquiry. What is the most ... Retrieved 12 February 2013.. *^ a b Plewa MC (March 2012). "Pediatric Aphthous Ulcers Treatment & Management". Medscape ... Retrieved 12 February 2013.. *^ Elad S, Epstein JB, von Bültzingslöwen I, Drucker S, Tzach R, Yarom N (March 2011). "Topical ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... InChI=1S/C8H10N4O2/c1-2-3-12-6-5(9-4-10-6)7(13)11-8(12)14/h4H,2-3H2,1H3,(H,9,10)(H,11,13,14) Y ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... Archived from the original on 2015-12-08.. *^ "WHO Model List of Essential Medicines (19th List)" (PDF). World Health ... InChI=1S/C20H30NO3.BrH/c1-14(2)21(3)16-9-10-17(21)12-18(11-16)24-20(23)19(13-22)15-7-5-4-6-8-15;/h4-8,14,16-19,22H,9-13H2,1-3H3 ... As a result, caution may be warranted, especially by men with prostatic hypertrophy.[12] ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... doi:10.1016/B978-0-12-408061-4.00004-3. ISBN 9780124080614. .. *^ "Caffeine". The Pharmacogenetics and Pharmacogenomics ... InChI=1S/C7H8N4O2/c1-10-3-8-5-4(10)6(12)9-7(13)11(5)2/h3H,1-2H3,(H,9,12,13) Y ... Caffeine is metabolized in the liver into three primary metabolites: paraxanthine (84%), theobromine (12%), and theophylline (4 ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... Studies reported in 2008, based on 12C/13C isotopic ratios of organic compounds found in the Murchison meteorite, suggested ... 12] synthesis, and reduce inflammation and innate immunity [12] and ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... Published June 12, 2009. Accessed March 1, 2017. *^ Artesunate Amodiaquine Winthrop (artesunate, amodiaquine) [summary of ... On June 12, 2009, the FDA concluded their review into the possibility of neuropsychiatric side effects with leukotriene ... 37-29/h3-12,14-16,20-21,32,40H,13,17-19,22-23H2,1-2H3,(H,38,39)/b15-10+/t32-/m1/s1 Y ...
The enzymes 15-lipoxygenase-1 (ALOX15 and 15-lipoxygenase-2 (ALOX15B metabolize arachidonic acid to 15- ... 5,8,11,14-all-cis-Eicosatetraenoic acid; all-cis-5,8,11,14-Eicosatetraenoic acid; Arachidonate ... The enzyme 5-lipoxygenase metabolizes arachidonic acid to 5-hydroperoxyicosatetraenoic acid (5-HPETE), which in turn is ... Zhu, D; Ran, Y (May 2012). "Role of 15-lipoxygenase/15-hydroxyeicosatetraenoic acid in hypoxia-induced pulmonary hypertension ...
Brain Res 100:99-110, 1975 Aveldano MI, Bazan NG: Rapid production of diacylglycerols enriched in arachidonate and stearate ... Eur J Biochem 145:21-29, 1984 Birkle DL, Bazan NG: Lipoxygenase and cyclooxygenase reaction products and incorporation into ... 6,653.311; Inventors: Nicolas G. Bazan, Carlos Sunkel, Julio Alvarez-Builla Gomez and Assignee: LSUHSC, "5-Lipoxygenase ... is metabolized to lipoxygenase reaction products in the retina. Biochem Biophys Res Comm 125:741-747, 1984 Beuckmann CT, Gordon ...
Arachidonate 5-lipoxygenase inhibitors. *Zileuton. Thromboxane receptor antagonists. *Ramatroban. *Seratrodast. Non-xanthine ... 2019-12-13. Retrieved 2020-03-28.. *^ a b Fox, C; Smith, T; Maidment, I; Chan, WY; Bua, N; Myint, PK; Boustani, M; Kwok, CS; ... Long-term use may increase the risk of both cognitive and physical decline.[11][12] It is unclear whether they affect the risk ... Prescriber's Letter 2011; 18 (12):271233. *^ "Anticholinergics", Anticholinergic Agents, Bethesda (MD): National Institute of ...
arachidonate (12S)-lipoxygenase. arachidonate (15S)-lipoxygenase. arachidonate 15-lipoxygenase,15S-type. linoleate 13S- ... polyunsaturated fatty acid lipoxygenase ALOX12. Names. 12S-lipoxygenase. Arachidonate 12-lipoxygenase, 12S-type. ... arachidonate 15-lipoxygenase activity IBA Inferred from Biological aspect of Ancestor. more info ... arachidonate 12-lipoxygenase, 12S typeprovided by HGNC. Primary source. HGNC:HGNC:429 See related. Ensembl:ENSG00000108839 MIM: ...
Arachidonate 12-Lipoxygenase) Homo sapiens, Authors: Sreeparna Banerjee, Asli Erdog. Published in: Atlas Genet Cytogenet Oncol ... Arachidonate lipoxygenase (ALOX) and cyclooxygenase (COX) polymorphisms and colon cancer risk.. Goodman JE, Bowman ED, Chanock ... lipoxygenases together with 5-lipoxygenase are also particularly high in tumours from patients who died of breast cancer. ... arachidonate 12-lipoxygenase activity iron ion binding protein binding cytoplasm cytosol cytosol aging positive regulation of ...
OMIM: ARACHIDONATE 12-OXIDOREDUCTASE; ALOX12*Gene Ontology: Alox12 *Mouse Phenome DB: Alox12 *UCSC: Chr.11:70,241,455- ... arachidonate 12-lipoxygenase. Synonyms: 9930022G08Rik, Alox12p, P-12LO. Gene nomenclature, locus information, and GO, OMIM, and ... Vega: OTTMUSG6035 (Alox12, arachidonate 12-lipoxygenase)*CCDS: 24942, 24942.1* ...
The role of lipoxygenases in epidermis.. 23621129. 2013. Non-syndromic autosomal recessive congenital ichthyosis in the Israeli ... Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega- ... This review covers the background to discovery of the two key lipoxygenases (LOX) involved in epidermal barrier function, 12R- ... It was shown that 12R-lipoxygenase is involved in MUC5AC expression via ERK- and Sp1-signalling pathways.. ...
Yoshimoto T, Suzuki H, Yamamoto S, Takai T, Yokoyama C, Tanabe T. Cloning and expression of arachidonate 12-lipoxygenase cDNA ... Yoshimoto, T. ; Suzuki, H. ; Yamamoto, S. ; Takai, T. ; Yokoyama, C. ; Tanabe, T. / Cloning and expression of arachidonate 12- ... Yoshimoto, T., Suzuki, H., Yamamoto, S., Takai, T., Yokoyama, C., & Tanabe, T. (1991). Cloning and expression of arachidonate ... Cloning and expression of arachidonate 12-lipoxygenase cDNA from porcine leukocytes.. T. Yoshimoto, H. Suzuki, S. Yamamoto, T. ...
Arachidonate lipoxygenases add oxygen molecules at different locations on the arachidonic acid molecule, producing a variety of ... This enzyme is part of a family of enzymes called arachidonate lipoxygenases. Most of these enzymes help add an oxygen molecule ... arachidonate 12-lipoxygenase, 12R type. Enable Javascript to view the expand/collapse boxes.. Printable PDF Open All Close All ... OMIM: ARACHIDONATE 12-LIPOXYGENASE, R TYPE. *. Eckl KM, Krieg P, Küster W, Traupe H, André F, Wittstruck N, Fürstenberger G, ...
Epidermal growth factor enhances transcription of human arachidonate 12-lipoxygenase in A431 cells. / Liu, Yi Wen; Chen, Ben ... Epidermal growth factor enhances transcription of human arachidonate 12-lipoxygenase in A431 cells. Biochimica et Biophysica ... Epidermal growth factor enhances transcription of human arachidonate 12-lipoxygenase in A431 cells. In: Biochimica et ... Fingerprint Dive into the research topics of Epidermal growth factor enhances transcription of human arachidonate 12- ...
Nugteren, D. H. (1975). "Arachidonate lipoxygenase in blood platelets". Biochimica et Biophysica Acta. 380 (2): 299-307. doi: ... Arachidonate 15-lipoxygenase-1 (15-LO-1, 15-LOX-1, ALOX15), which is expressed in far more tissues that ALOX12, metabolizes ... sequence indetity to mouse Arachidonate 8-lipoxygenase. Mouse e-12LO metabolizes arachidonic acid predominantly to 12(S)-HETE ... "Arachidonate dilates basilar artery by lipoxygenase-dependent mechanism and activation of K(+) channels". American Journal of ...
ALOXE3: arachidonate lipoxygenase 3. *ALPL: alkaline phosphatase, biomineralization associated. *ALS2: ALS2, alsin Rho guanine ...
Linoleate 9S-lipoxygenase 2. Solanum tuberosum (Potato). Loading... O35936 Arachidonate 8S-lipoxygenase. Mus musculus (Mouse). ... Manganese lipoxygenase. Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus). Loading... ...
ARACHIDONATE LIPOXYGENASE 3; ALOXE3. 607800. ATP-BINDING CASSETTE, SUBFAMILY A, MEMBER 12; ABCA12. ... The twelve genes known to be associated with ARCI are ABCA12, ALOX12B, ALOXE3, CASP14, CERS3, CYP4F22, LIPN, NIPAL4, PNPLA1, ... Arachidonate 12-lipoxygenase, 12R-type. ALOX12B database. ALOX12B. ALOX12B. ALOXE3. 17p13. ​.1. Hydroperoxide isomerase ALOXE3 ... Normal gene product. The protein product of ALOX12B, the enzyme arachidonate 12-lipoxygenase, 12R-type (12R-LOX), has 701 amino ...
Twelve artemisinin-based compounds and scopoletin have been included in the analysis. None of the compounds were commonly found ... Arachidonate 12-lipoxygenase RNA. Inflammation, carcinogenesis, membrane remodeling. 0.399. GC32483. M32313. SRD5A1. Steroid-5- ... Yang, Y.F.; Xu, W.; Song, W.; Ye, M.; Yang, X.W. Transport of twelve coumarins from angelicae pubescentis radix across a mdck- ... Genome Biol. 2011, 12, R83. [Google Scholar] [CrossRef] [PubMed]. *Berman, H.M.; Westbrook, J.; Feng, Z.; Gilliland, G.; Bhat, ...
EC (arachidonate 15-lipoxygenase) inhibitor A lipoxygenase inhibitor that interferes with the action of arachidonate ... baicalein (CHEBI:2979) has role EC (arachidonate 15-lipoxygenase) inhibitor (CHEBI:64996) baicalein (CHEBI:2979) has ... A lipoxygenase inhibitor that interferes with the action of arachidonate 12-lipoxygenase (EC ... baicalein (CHEBI:2979) has role EC (arachidonate 12-lipoxygenase) inhibitor (CHEBI:64995) ...
arachidonate 12-lipoxygenase, 12R type. 98 203575_at. casein kinase 2, alpha prime polypeptide. ... 12. (canceled) 13. (canceled) 14. The method of claim 6, wherein the method further comprises taking a biopsy of the target ... 12. 224570_s_at. interferon regulatory factor 2 binding protein 2. 13. 224571_at. interferon regulatory factor 2 binding ...
1999) Arachidonate 12-lipoxygenase isozymes. Adv Exp Med Biol 447:37-44. ... Growth Cone Collapse Induced by Semaphorin 3A Requires 12/15-Lipoxygenase. Keith Mikule, Jesse C. Gatlin, Becky A. de la ... Growth Cone Collapse Induced by Semaphorin 3A Requires 12/15-Lipoxygenase. Keith Mikule, Jesse C. Gatlin, Becky A. de la ... Sema3A and 12(S)-HETE treatment of DRG growth cones causes a loss of adhesive area. IRM images were taken before (left) and ...
Arachidonate 12-lipoxygenases YOSHIMOTO T Prostaglandins Other Lipid Mediat. 68-69, 245-262, 2002 ... ロイコトリエン・リポキシゲナーゼ代謝系と創薬 [in Japanese] Leukotriene-lipoxygenase pathway and drug discovery [in Japanese] * * 阿部 正義 ABE Masayoshi ... Zileuton, a 5-lipoxygenase inhibitor, increases production of thromboxane A_2 and platelet aggregation in patients with asthma ... Since the 5-lipoxygenase pathway is abundantly expressed in atherosclerotic lesions, and 12/15-lipoxygenase is able to ...
Buy our Recombinant Human 12 Lipoxygenase protein. Ab112250 is a full length protein produced in Wheat germ and has been ... Arachidonate 12 lipoxygenase, 12S type. *Arachidonate 12-lipoxygenase. *Arachidonate 12-oxidoreductase. *Lipoxin synthase 12-LO ... Belongs to the lipoxygenase family.. Contains 1 lipoxygenase domain.. Contains 1 PLAT domain. ... Recombinant Human 12 Lipoxygenase protein. See all 12 Lipoxygenase proteins and peptides. ...
15-Lipoxygenase is now under investigations.. 3) 12-Lipoxygenase of human uterine cervix : Nigams group of Free University of ... Publications] S.Yamamoto and W.L.Smith (eds.): Elsevier Science B.V.Molecular Biology of the Arachidonate Cascade, 1-454 (1995) ... Publications] I.Feussner: Lipoxygenase catalysed oxygenation of storage lipids is implicated in lipid mobilization during ... Publications] I.Feussner: Lipoxygenase catalysed oxygenation of storage lipids is implicated in lipid mobilization during ...
Arachidonate 5-lipoxygenase, also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13 ... that in humans is encoded by the ALOX5 gene.[5] Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. ... "Alox5 - arachidonate 5-lipoxygenase". WikiGenes.. *^ Fahel JS, de Souza MB, Gomes MT, Corsetti PP, Carvalho NB, Marinho FA, de ... arachidonate 5-lipoxygenase activity. • dioxygenase activity. • metal ion binding. • protein binding. • oxidoreductase activity ...
"Entrez Gene: ALOX12B arachidonate 12-lipoxygenase, 12R type". Boeglin WE, Kim RB, Brash AR (June 1998). "A 12R-lipoxygenase in ... Among the human lipoxygenases, ALOX12B is most closely (54% identity) related in amino acid sequence to ALOXE3 ALOX12B ... Arachidonate 12-lipoxygenase, 12R type, also known as ALOX12B, 12R-LOX, and arachiconate lipoygenase 3, is a lipoxygenase-type ... The gene is located on chromosome 17 at position 13.1 where it forms a cluster with two other lipoxygenases, ALOXE3 and ALOX15B ...
ALOX12 (fold change = 2.29; p = 0.0323) is a lipoxygenase that contributes to the epidermal barrier function. When a decrease ... ALOX12, arachidonate 12-lipoxygenase; DHCR24, 24-dehydrocholesterol reductase; DUOX1, dual oxidase 1; GPX2, glutathione ... The comparison revealed 10 upregulated genes as follows: arachidonate 12-lipoxygenase (ALOX12), 24-dehydrocholesterol reductase ... Ten significantly upregulated genes were identified as follows: arachidonate 12-lipoxygenase (ALOX12), 24-dehydrocholesterol ...
Rabbit recombinant monoclonal 12 Lipoxygenase antibody [EPR11380(B)] validated for WB and tested in Human and Rat. Immunogen ... Arachidonate 12 lipoxygenase antibody. *Arachidonate 12 lipoxygenase, 12S type antibody. *Arachidonate 12-lipoxygenase antibody ... Belongs to the lipoxygenase family.. Contains 1 lipoxygenase domain.. Contains 1 PLAT domain. ... All lanes : Anti-12 Lipoxygenase antibody [EPR11380(B)] (ab168384) at 1/1000 dilution. Lane 1 : A431 lysate. Lane 2 : Human ...
Formation of the mammalian skin barrier requires both the essential fatty acid linoleate and the two lipoxygenases 12R- ... lipoxygenase (12R-LOX) and epidermal lipoxygenase-3 (eLOX3), alth ... EC 12-Lipoxygenase Comments/Corrections ... Arachidonate 12-Lipoxygenase / genetics, metabolism*. Ceramides / genetics, metabolism*. Epidermis / metabolism*. Fatty Acids, ... Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega- ...
Arachidonate lipoxygenase 3. Arachidonate 12-lipoxygenase, 12R type Epidermolytic hyperkeratosis (bullous ichthyosis, bCIE) ...
ALOXE3: arachidonate lipoxygenase 3. *ALPL: alkaline phosphatase, biomineralization associated. *ALS2: ALS2, alsin Rho guanine ...
2. Nugteren, D.H. Arachidonate lipoxygenase in blood platelets. Biochim. Biophys. Acta 380 (1975) 299-307. [PMID: 804329]. 3. ... lipoxygenase; leukotriene A4 synthase; LTA4 synthase. Systematic name: arachidonate:oxygen 12-oxidoreductase. Comments: The ... Reaction: arachidonate + O2 = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate. Other name(s): δ12-lipoxygenase ... Characteristics and inhibition by a variety of phenyl hydrazones and comparisons with other lipoxygenases. Biochim. Biophys. ...
ALOX15B; arachidonate 15-lipoxygenase, type B [KO:K08022] [EC:1.13.11.-]. ... ALOX12; arachidonate 12-lipoxygenase, 12S type [KO:K00458] [EC:]. 242 ALOX12B; arachidonate 12-lipoxygenase, 12R type ... Identification of the 11,14,15- and 11,12, 15-trihydroxyeicosatrienoic acids as endothelium-derived relaxing factors of rabbit ...
Arachidonate 15-Lipoxygenase, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The ... ALOX15 (Arachidonate 15-Lipoxygenase) is a Protein Coding gene. Diseases associated with ALOX15 include Asthma, Nasal Polyps, ... arachidonate-15-lipoxygenase,lipid peroxidating enzyme implicated in the pathogenesis of inflammatory disorders as well as ... Orally active 5-lipoxygenase activating protein (FLAP) inhibitor. 128253-31-6. BW-B 70C. 5-Lipoxygenase inhibitor. 134470-38-5 ...
Arachidonate 15-Lipoxygenase Inhibitors (0) see Lipoxygenase Inhibitors. Arachidonate 5-Lipoxygenase Inhibitors (0) see ... Lipoxygenase Inhibitors. Aromatase Inhibitors (10) • Compounds that inhibit AROMATASE in order to reduce production of ... Amebicides (12) • Agents which are destructive to amebae, especially the parasitic species causing AMEBIASIS in man and animal ...
  • According to NCBI Map Viewer, genes flanking ALOX15 in centromere to telomere direction on 17p13 are: GABARAP 17p13.1 GABA(A) receptor-associated protein, ASGR2 asialoglycoprotein receptor 2, ALOX12 17p13.1 arachidonate 12-lipoxygenase (Homo sapiens), ALOX12P2 17p13 arachidonate 12-lipoxygenase pseudogene 2, TEKT1 tektin 1, FBXO39 F-box protein 39. (
  • In humans, Arachidonate 12-lipoxygenase (12-LO, 12-LOX, ALO12, or platelet type 12-lipoxygenase) is encoded by the ALOX12 gene and expressed primarily in platelets and skin. (
  • ALOX12 metabolizes arachidonic acid almost exclusively to 12(S)-hydroperoxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (12(S)-HpETE or 12S-HpETE). (
  • Recent studies indicate that the metabolism by ALOXE3 of the R stereoisomer of 12-HpETE made by ALOX12B and therefore possibly the S stereoisomer of 12-HpETE made by ALOX12 or ALOX15 is responsible for forming various hepoxilins in the epidermis of human and mouse skin and tongue and possibly other tissues. (
  • ALOX12 (arachidonate 12-lipoxygenase) gene is mapped to human chromosome 17p13.1. (
  • Jobard F, Lefèvre C, Karaduman A, Blanchet-Bardon C, Emre S, Weissenbach J, Ozgüc M, Lathrop M, Prud'homme JF, Fischer J. Lipoxygenase-3 (ALOXE3) and 12(R)-lipoxygenase (ALOX12B) are mutated in non-bullous congenital ichthyosiform erythroderma (NCIE) linked to chromosome 17p13.1. (
  • Arachidonate 12-lipoxygenase, 12R type, also known as ALOX12B, 12R-LOX, and arachiconate lipoygenase 3, is a lipoxygenase-type enzyme composed of 701 amino acids and encoded by the ALOX12B gene. (
  • Among the human lipoxygenases, ALOX12B is most closely (54% identity) related in amino acid sequence to ALOXE3 ALOX12B oxygenates arachidonic acid by adding molecular oxygen (O2) in the form of a hydroperoxyl (HO2) residue to its 12th carbon thereby forming 12(R)-hydroperoxy-5Z,8Z,10E,14Z-icosatetraenoic acid (also termed 12(R)-HpETE or 12R-HpETE). (
  • These sequential metabolic reactions are: arachidonic acid + O2 ⇌ {\displaystyle \rightleftharpoons } 12R-HpETE → 12R-HETE ALOX12B is also capable of metabolizing free linoleic acid to 9(R)-hydroperoxy-10(E),12(Z)-octadecadienoic acid (9R-HpODE) which is also rapidly converted to its hydroxyl derivative, 9-Hydroxyoctadecadienoic acid (9R-HODE). (
  • Porcine Arachidonate 12-lipoxygenase, 12R-type (ALOX12B) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. (
  • Based on structural and enzymatic properties, 15-LOX-1 is said to be a homolog of leukocyte type 12-LOX and are both expressed in mast cells, eosinophils, activated monocytes or dendritic cells, and bronchial epithelial cells. (
  • Sub-primate mammals, such as the mouse, rat, rabbit, cow, and pig, express platelet type 12-lipoxygenase but also a leukocyte type 12-lipoxygenase (also termed 12/15-lipoxygenase, 12/15-LOX or 12/15-LO) which is an ortholog of, and metabolically equivalent to, human 15-LO-1 in that it forms predominantly 15(S)-HpETE with 12(S)-HpETE as a minor product. (
  • A lipoxygenase inhibitor that interferes with the action of arachidonate 12-lipoxygenase (EC (
  • 12-LOX protein consists of 662 amino acids, with a molecular weight of 75536 Da and contains non heme iron as a cofactor. (
  • According to the NCBI conserved domain search, the presence of a polycystin/lipoxygenase/alpha-toxin (PLAT) domain in the 12-LOX protein allows it access and enables it to catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and plasma lipoproteins. (
  • Epidermal growth factor (EGF), determined by immunoprecipitation and Western blot analysis, increased both enzyme activity and protein level of 12-lipoxygenase in the solubilized microsomes of human epidermoid carcinoma A431 cells, respectively. (
  • The time-dependent induction of luciferase activity by EGF paralleled the EGF-induced enzyme activity and expression of 12-lipoxygenase protein. (
  • Several lines of evidence suggested that the 14,15-epoxy acid was covalently incorporated into the enzyme protein and the leukocyte 12-lipoxygenase was suicide-inactivated. (
  • The ALOX5 gene, which occupies 71.9 kilo base pairs (kb) on chromosome 10 (all other human lipoxygenases are clustered together on chromosome 17), is composed of 14 exons divided by 13 introns encoding the mature 78 kilodalton (kD) ALOX5 protein consisting of 673 amino acids. (
  • ALOX15 (Arachidonate 15-Lipoxygenase) is a Protein Coding gene. (
  • The 15- and 5- but not 12-LO protein expressions were greater in females. (
  • The recombinant protein was identified as arachidonate 12-lipoxygenase and linoleate 13-lipoxygenase with a preference for arachidonic acid and eicosapentaenoic acid. (
  • Specifically, 12(S)-HETE enhanced the activation of protein kinase C by phorbol esters, mimicked phorbol ester-induced adhesion of keratinocytes to fibronectin and mimicked phorbol ester repression of expression of a differentiation-related gene, keratin-1. (
  • Mutation spectrum and functional analysis of epidermis-type lipoxygenases in patients with autosomal recessive congenital ichthyosis. (
  • The importance of the lipoxygenase-hepoxilin pathway in the mammalian epidermal barrier. (
  • The expression of epidermal lipoxygenases and transglutaminase-1 is perturbed by NIPAL4 mutations: indications of a common metabolic pathway essential for skin barrier homeostasis. (
  • The initial 12(S)-HpETE and 12(R)-HpETE products, regardless of their pathway of formation, are rapidly reduced to 12(S)-HETE and 12(R)-HETE, respectively, by ubiquitous cellular peroxidases, including in particular Glutathione peroxidases or, alternatively, are further metabolized as described below. (
  • The present studies on DRG neurons test the hypothesis that at least one of the Semas, Sema3A, also uses a signaling pathway involving 12/15-LO to trigger growth cone collapse and that this pathway affects growth cone adhesion. (
  • Since the 5-lipoxygenase pathway is abundantly expressed in atherosclerotic lesions, and 12/15-lipoxygenase is able to oxygenate polyunsaturated fatty acid esterified in the membranous phospholipids, 5-lipoxygenase or 12/15-lipoxygenase inhibitors may prevent progression of atherosclerosis. (
  • Arachidonate 12-lipoxygenase is a non-heme iron-containing dioxygenase that functions in the leukotrienes pathway. (
  • We also show that the toxic actions of this biocide are zinc dependent and require the activation of p44/42 extracellular signal-regulated kinase (ERK) via a 12-lipoxygenase-mediated pathway. (
  • From arachidonic acid (AA), it leads to proaggregatory prostanoids, prostaglandin endoperoxides (PGG 2 /PGH 2 ) and thromboxane A 2 (TxA 2 ), which together with 12-hydroxyheptadecatrienoic acid represent the main compounds of the pathway, primary prostaglandins being minor products. (
  • Less data about platelet lipoxygenase pathway, especially for its biological role, are available. (
  • Apart from species, strain, early differences in glycemic control, and/or dosing effects, the failure to modulate albuminuria may have been due to a decrement in renal HSP25 or stimulation of the 12/15 lipoxygenase pathway in DBA2J mice fed curcumin. (
  • Separate lines of research have identified the 5-lipoxygenase/leukotriene B 4 receptor pathway and the PPAR pathway as potential targets for prevention or treatment of this disease. (
  • The third pathway for metabolizing arachidonic acid, the lipoxygenase pathway catalyzes the incorporation of one oxygen molecule into polyunsaturated fatty acids to yield a 1-hydroperoxy-2, 4-trans, cis-pentadiene product [ 14 - 16 ]. (
  • Arachidonate lipoxygenases add oxygen molecules at different locations on the arachidonic acid molecule, producing a variety of substances called fatty acid hydroperoxides. (
  • 12-Hydroxyeicosatetraenoic acid (12-HETE) is a derivative of the 20 carbon polyunsaturated fatty acid, arachidonic acid, containing a Hydroxyl residue at carbon 12 and a 5Z,8Z,10E,14Z Cis-trans isomerism configuration (Z=cis, E=trans) in its four double bonds. (
  • It was first found as a product of arachidonic acid metabolism made by human and bovine platelets through their 12S-lipoxygenase (i.e. (
  • it metabolizes arachidonic acid to 12(R)-HpETE. (
  • Mouse e-12LO metabolizes arachidonic acid predominantly to 12(S)-HETE and to a lesser extent 15(S)-HETE. (
  • The leukocyte enzyme produced a small amount of 15-hydroperoxy acid from arachidonic acid in addition to 12-hydroperoxy acid. (
  • Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. (
  • A significant increase of 12-lipoxygenase enzyme activity was observed in the presence of arachidonic acid (AA) under GSH depletion induced by glutamate. (
  • Possible vasoconstrictor mediators include arachidonic acid-derived lipoxygenase (LO) metabolites. (
  • It converts arachidonic acid to12(S)-hydroperoxyeicosatetraenoic acid, which is then converted to 12(S)-hydroxyeicosatetraenoic acid. (
  • When platelets are aggregated by some of these agents such as thrombin or collagen, polyunsaturated fatty acids (mainly arachidonic acid) are liberated from membrane phospholipids and then subsequently oxygenated via cyclooxygenase and/or lipoxygenase. (
  • Because of these unique features the pronounced formation of (2Z)-octen-1-ol, 1-octen-3-ol, the dienal (5Z,8Z,10E)-12-oxo-dodecatrienoic acid and 12-keto eicosatetraenoic acid was observed when arachidonic acid was administered as substrate. (
  • Lipoxygenases catalyze the conversion of poly-unsaturated fatty acids in the plasma membrane-mainly arachidonic acid and linoleic acid-to produce oxidized pro-inflammatory lipid intermediates. (
  • The gene is located on chromosome 17 at position 13.1 where it forms a cluster with two other lipoxygenases, ALOXE3 and ALOX15B. (
  • Arachidonate 12-lipoxygenase and 12-hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer. (
  • Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega-hydroxyceramide for construction of the corneocyte lipid envelope. (
  • Mice also express an epidermal type 15-lipoxygenase (e-12LO) which has 50.8% amino acid sequence identity to human 15-LOX-2 and 49.3% sequence indetity to mouse Arachidonate 8-lipoxygenase. (
  • 12(R)-HpETE also spontaneously decomposes to a mixture of hepoxilins and trihydroxy-eicosatetraenoic acids that possess R or S hydroxy and epoxy residues at various sites while 8R-hydroxy,11R,12R-epoxy-hepoxilin A3 spontaneously decomposes to 8R,11R,12R-trihydroxy-5Z,9E,14Z-eicosatetraenoic acid. (
  • In cultures of dorsal root ganglion neurons, we found that Sema3A treatment stimulates the synthesis of the eicosanoid, 12(S)-hydroxyeicosatetraenoic acid (HETE), whereas Sema3A-induced growth cone collapse is prevented when 12(S)-HETE synthesis is blocked with an inhibitor of 12/15-lipoxygenase (LO). (
  • When formed in cells, 12R-HpETE may be quickly reduced to its hydroxyl analog (OH), 12(R)-hydroxy-5'Z,8Z,10E,14Z-eicosatetraenoic acid (also termed 12(R)-HETE or 12R-HETE), by ubiquitous peroxidase-type enzymes. (
  • Formation of the mammalian skin barrier requires both the essential fatty acid linoleate and the two lipoxygenases 12R-lipoxygenase (12R-LOX) and epidermal lipoxygenase-3 (eLOX3), although their roles are poorly understood. (
  • Lipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty acid hydroperoxidases. (
  • It has been reported that 12-hydroperoxyeicosatetraenoic acid (12-HPETE), the lipoxygenase product of AA, is able to inhibit both thromboxane synthase (Hammarström and Falardeau, 1977) and platelet diglyceride lipase (Rittenhouse-Simmons, 1980). (
  • In contrast to any other lipoxygenase cloned so far, this enzyme exhibited in addition an unusual high hydroperoxidase and also a fatty acid chain-cleaving lyase activity. (
  • 12-Hydroperoxy eicosatetraenoic acid was found to be only a minor product. (
  • This effect was ascribed in part to increased expression of 12-lipoxygenase (12-LOX) and its arachidonate metabolite 12-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid (12-HETE). (
  • MATERIALS AND METHODS: In this study, we synthesized a series of novel cyclic hydroxamic acid compounds that demonstrated varying degrees of inhibitory effects on the arachidonate 12-lipoxygenase. (
  • A comparison of the arachidonate lipoxygenase metabolite 12-hydroxyeicosatetraenoic acid (12-HETE) with the 13-hydroxyoctadecadienoic acid (13-HODE) lipoxygenase metabolite of linoleate revealed that 12-HETE has biological activities that mimic the phorbol ester tumor promoters, whereas 13-HODE has antithetical effects. (
  • Thromboxane A synthase 1 (platelet) ( THAS ) forms 12-hydroxyheptadeca-5,8,10-trienoic acid and malonic dialdehyde as a byproduct, Thromboxane A(,2 ) [ 38 ], [ 39 ] and Thromboxane B2 . (
  • during this metabolism, however, ALOX15 also forms 12(S)-HpETE as a minor product. (
  • The cloning of genes related to lipoxygenase-leukotriene metabolism prompted us to try to elucidate the role of leukotrienes in various inflammations. (
  • In conclusion, the leukotriene-lipoxygenase metabolism may be involved in the pathophysiology of acute inflammatory to chronic progressive disorders. (
  • We think that more drugs modifying leukotriene-lipoxygenase metabolism will be introduced into clinical practice in the future. (
  • A lipoxygenase inhibitor that interferes with the action of arachidonate 15-lipoxygenase (EC (
  • The adhesion site effect seems partially independent of the actin cytoskeleton because growth cones treated with Sema3A and 12/15-LO inhibitor remain spread despite actin cytoskeleton loss. (
  • 7 , - , 12 However, in our previous study, pretreatment with the cyclooxygenase inhibitor indomethacin only partially blocked contractions in the females compared with an almost complete attenuation in the males. (
  • 6 The combined cyclooxygenase/lipoxygenase (LO) inhibitor BW 755C completely blocked contractions in the females. (
  • Baicalein is also known as a selective 12-lipoxygenase (12-LOX) inhibitor ( 1 ), which induces antiproliferation and apoptosis in various cancer cells ( 2 - 4 ). (
  • More recently, 12-HPETE was described as an inhibitor of PGH 2 /TxA 2 -induced platelet aggregation (Aharony et al . (
  • The EGF-induced expression of 12-lipoxygenase mRNA was inhibited by transcription inhibitors such as actinomycin D and 5,6-dichlorobenzimidazole riboside. (
  • Taken together, the results of this study indicate that EGF enhanced the transcription of the human 12-lipoxygenase gene, resulting in an increase in the amount and activity of 12-lipoxygenase. (
  • Arachidonate has protumor-promoting action that is inhibited by linoleate in mouse skin carcinogenesis. (
  • The goal of this study was to determine whether the arachidonate content of epidermal phospholipids could be altered by increasing dietary levels of linoleate and whether specific metabolites of linoleate and arachidonate have dissimilar biological effects. (
  • In a series of tumor studies in which the quantity of dietary linoleate was incrementally increased, a slight reduction in phospholipid levels of arachidonate was observed that correlated with an increased phospholipid level of linoleate and a suppression in tumor yield. (
  • Overall, these studies suggest that arachidonate and linoleate have opposing functions in the epidermis, particularly with regard to events involved in tumor development. (
  • Cyclooxygenase is well known asan enzyme responsible for the biosynthesis of prostaglandins and thromboxanes, and 5-lipoxygenase initiates the leukotriene synthesis. (
  • Publications] T.Ohtsuki: 'Reperfusion induces 5-lipoxygenase translocation and leukotriene C_4 Production in ischemic brain' Am.J.Physiol.268. (
  • Common involved genes include transglutaminase 1 ( TGM1 ), arachidonate 12-lipoxygenase ( ALOXE12B ), arachidonate lipoxygenase 3 ( ALOX3 ), and ATP-binding cassette transporter 12 ( ABCA12 ). (
  • 12S-lipoxygenases has three isoforms, named after their site of initial identification: platelet, leukocyte and epidermis. (
  • The role of lipoxygenases in epidermis. (
  • Krieg P, Fürstenberger G. The role of lipoxygenases in epidermis. (
  • Cloning and expression of arachidonate 12-lipoxygenase cDNA from porcine leukocytes. (
  • Cloning and sequence analysis of the cDNA for arachidonate 12-lipoxygenase of porcine leukocytes. (
  • Sequence analysis of the cDNA showed that it exhibits a domain structure similar to that of type2 lipoxygenases from plants, harboring an N-terminal import signal for chloroplasts. (
  • Arachidonate 5-lipoxygenase , also known as ALOX5 , 5-lipoxygenase , 5-LOX , or 5-LO , is a non- heme iron-containing enzyme (EC that in humans is encoded by the ALOX5 gene . (
  • In addition, the platelet-type 12S-lipoxygenase was found in both cytosol and microsomal fractions of epidermal cells of human skin. (
  • Probing the Electrostatic and Steric Requirements for Substrate Binding in Human Platelet-Type 12-Lipoxygenase. (
  • This is the 'epidermal type' 12-LOX (e-12LO) that was cloned using a murine e-LO12 probe. (
  • The platelet type 12-LOX is expressed in the platelets and skin in humans. (
  • These findings indicated that the 12-lipoxygenase of human uterine cervix was of platelet-type. (
  • Publications] M.Nakamura: 'Immunocytochemical localization of platelet-type arachidonate 12-lipoxygenase in mouse blood cells' J.Histochem.Cytochem.43. (
  • This gene encodes a member of the lipoxygenase family of proteins. (
  • This enzyme is part of a family of enzymes called arachidonate lipoxygenases. (
  • [5] Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. (
  • CYP450 enzymes are monooxygenases functionally related to other oxygenases, such as monoamine oxygenase and the lipoxygenase family [23, 24]. (
  • Synthetic peptide, corresponding to residues in Human 12 Lipoxygenase (UniProt: P18054). (
  • 3. Wallach, D.P. and Brown, V.R. A novel preparation of human platelet lipoxygenase. (
  • Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/Apo2L) selectively induces apoptosis in various cancer cells in vitro and in vivo , with little or no toxicity in normal cells ( 9 - 12 ). (
  • Overall, our data suggested that dietary sugar induces 12-LOX signaling to increase risks of breast cancer development and metastasis. (
  • Molecular structure and function of the porcine arachidonate 12-lipoxygenase gene. (
  • To investigate the formation of these oxylipins at the molecular level we isolated a lipoxygenase from P. patens, which was identified in an EST library by sequence homology to lipoxygenases from plants. (
  • R) antagonists are preferred as anti-asthma and anti-rhinitis drugs because they are almost as effective as the 5-lipoxygenase inhibitors but have fewer side effects. (
  • Omega 3 fatty acids inhibit tumor growth by a number of mechanisms including suppression of COX-2 expression and, for EPA at least, the alternative substrate produces different cyclooxygenase (PGE 3 ) and lipoxygenase (LTB 5 ) products that have anti-inflammatory and anticancer effects. (
  • Sub-human primates, although not extensively examined, appear to have 12-lipoxygenase expression patterns that resemble those of sub-primate mammals or humans depending on the closeness of there genetic relateness to these species. (
  • LY293111 was also known to have inhibitory effects on 5-lipoxygenase, which is upstream of the production of leukotrienes. (
  • The enzyme activity in the supernatant was immunoprecipitated by the use of anti-human platelet 12-lipoxygenase antibody. (
  • Owing to the similarities in their genetic location, sequence and biological activities, leukocyte 12-LOX and 15-LOX-1 are often referred to as 12/15 lipoxygenase. (