AraC Transcription Factor
Monosaccharides
Galactans
Actinomycetales
Genes, araC
Carbohydrates
Galactose
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Carbohydrate Metabolism
RNA, Ribosomal, 16S
Cell Wall
Fermentation
DNA, Ribosomal
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Micromonosporaceae
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Gene Expression Regulation, Bacterial
Rhamnose
Soil Microbiology
Operon
Xylosidases
A group of enzymes that catalyze the hydrolysis of alpha- or beta-xylosidic linkages. EC 3.2.1.8 catalyzes the endo-hydrolysis of 1,4-beta-D-xylosidic linkages; EC 3.2.1.32 catalyzes the endo-hydrolysis of 1,3-beta-D-xylosidic linkages; EC 3.2.1.37 catalyzes the exo-hydrolysis of 1,4-beta-D-linkages from the non-reducing termini of xylans; and EC 3.2.1.72 catalyzes the exo-hydrolysis of 1,3-beta-D-linkages from the non-reducing termini of xylans. Other xylosidases have been identified that catalyze the hydrolysis of alpha-xylosidic bonds.
Gordonia Bacterium
Bacterial Typing Techniques
Sequence Analysis, DNA
Lignin
The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)
Transcription Initiation, Genetic
Root Nodules, Plant
Artificial Gene Fusion
Endo-1,4-beta Xylanases
Clostridium acetobutylicum
Pectins
High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.
Glucose
Vitamin K 2
Fatty Acids
Uronic Acids
Xylitol
Genes, rRNA
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
The Salmonella typhi melittin resistance gene pqaB affects intracellular growth in PMA-differentiated U937 cells, polymyxin B resistance and lipopolysaccharide. (1/706)
Salmonella typhi is the causative agent of typhoid fever in humans. A cell-culture based assay involving the human monocyte macrophage cell line U937 has been developed to examine S. typhi invasion and survival. An S. typhi PhoP- (null) mutant was shown to be restricted in net growth in phorbol myristate acetate (PMA) differentiated U937 (PMA-U937) cells, and an S. typhi PhoPc (constitutive) mutant showed a defect in invasion. Neither of the phoP/Q mutants were growth impaired in HeLa cells, however the PhoPc mutant was impaired in invasion. As opposed to what was found for S. typhi, Salmonella typhimurium wild-type, PhoP- and PhoPc mutants grew equally well in PMA-U937 cells, indicating that the PhoP(-)-mediated net growth restriction in the PMA-U937 cells was S. typhi specific. An S. typhi mutation, pqaB::MudJ, recently shown to be a PhoP-activated locus, was shown to have a net growth defect in PMA-U937 cells. Sequencing of the S. typhipqaB gene revealed it had 98% identity to the fifth gene in a S. typhimurium PmrA/B regulated operon necessary for 4-aminoarabinose lipid A modification and polymyxin B resistance. The pqaB locus was regulated by PmrA/B (whose activity is modulated by PhoP-PhoQ) and the pqaB transposon mutant was sensitive to polymyxin B. The lipopolysaccharides (LPS) of S. typhi and S. typhimurium wild-type, PhoP- and PhoPc mutants, were compared by SDS-PAGE and silver staining. Differences in the LPS profile between the two Salmonella species were observed, and shown to be affected differently by the PhoPc mutation. Additionally, the pqaB::MudJ mutation affected S. typhi LPS. The effects on LPS may have ramifications for the difference between S. typhi and S. typhimurium infection of hosts. (+info)Effect of 9-beta-D-arabinofuranosyladenine 5'-monophosphate and 9-beta-D-arabinofuranosylhypoxanthine 5'-monophosphate on experimental herpes simplex keratitis. (2/706)
Treatment of established experimental keratitis caused by herpes simplex virus with 9-beta-d-arabinofuranosyladenine 5'-monophosphate (Ara-AMP) or 9-beta-d-arabinofuranosylhypoxanthine 5'-monophosphate (Ara-HxMP) showed that the Ara-AMP, in a concentration of 2 or 20%, had a significant effect on the keratitis but that 0.4% Ara-HxMP showed only minimal activity. Ara-AMP was also effective in the treatment of idoxuridine-resistant keratitis. No local toxicity with a high concentration (20%) of Ara-AMP was seen, but the duration of therapy was brief. (+info)Transcriptional activation of ydeA, which encodes a member of the major facilitator superfamily, interferes with arabinose accumulation and induction of the Escherichia coli arabinose PBAD promoter. (3/706)
Induction of genes expressed from the arabinose PBAD promoter is very rapid and maximal at low arabinose concentrations. We describe here two mutations that interfere with the expression of genes cloned under arabinose control. Both mutations map to the ydeA promoter and stimulate ydeA transcription; overexpression of YdeA from a multicopy plasmid confers the same phenotype. One mutation is a large deletion that creates a more efficient -35 region (ATCACA changed to TTCACA), whereas the other affects the initiation site (TTTT changed to TGTT). The ydeA gene is expressed at extremely low levels in exponentially growing wild-type cells and is not induced by arabinose. Disruption of ydeA has no detectable effect on cell growth. Thus, ydeA appears to be nonessential under usual laboratory growth conditions. The ydeA gene encodes a membrane protein with 12 putative transmembrane segments. YdeA belongs to the largest family of bacterial secondary active transporters, the major facilitator superfamily, which includes antibiotic resistance exporters, Lac permease, and the nonessential AraJ protein. Intracellular accumulation of arabinose is strongly decreased in mutant strains overexpressing YdeA, suggesting that YdeA facilitates arabinose export. Consistent with this interpretation, very high arabinose concentrations can compensate for the negative effect of ydeA transcriptional activation. Our studies (i) indicate that YdeA, when transcriptionally activated, contributes to the control of the arabinose regulon and (ii) demonstrate a new way to modulate the kinetics of induction of cloned genes. (+info)Phylogenetic analysis of Ara+ and Ara- Burkholderia pseudomallei isolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes. (4/706)
A Burkholderia pseudomallei-like organism has recently been identified among some soil isolates of B. pseudomallei in an area with endemic melioidosis. This organism is almost identical to B. pseudomallei in terms of morphological and biochemical profiles, except that it differs in ability to assimilate L-arabinose. These Ara+ isolates are also less virulent than the Ara- isolates in animal models. In addition, clinical isolates of B. pseudomallei available to date are almost exclusively Ara-. These features suggested that these two organisms may belong to distinctive species. In this study, the 16S rRNA-encoding genes from five clinical (four Ara- and one Ara+) and nine soil isolates (five Ara- and four Ara+) of B. pseudomallei were sequenced. The nucleotide sequences and phylogenetic analysis indicated that the 16S rRNA-encoding gene of the Ara+ biotype was similar to but distinctively different from that of the Ara- soil isolates, which were identical to the classical clinical isolates of B. pseudomallei. The nucleotide sequence differences in the 16S rRNA-encoding gene appeared to be specific for the Ara+ or Ara- biotypes. The differences were, however, not sufficient for classification into a new species within the genus Burkholderia. A simple and rapid multiplex PCR procedure was developed to discriminate between Ara- and Ara+ B. pseudomallei isolates. This new method could also be incorporated into our previously reported nested PCR system for detecting B. pseudomallei in clinical specimens. (+info)Substrate sequestration by a proteolytically inactive Lon mutant. (5/706)
Lon protein of Escherichia coli is an ATP-dependent protease responsible for the rapid turnover of both abnormal and naturally unstable proteins, including SulA, a cell division inhibitor made after DNA damage, and RcsA, a positive regulator of transcription. Lon is a multimer of identical 94-kDa subunits, each containing a consensus ATPase motif and a serine active site. We found that overexpressing Lon, which is mutated for the serine active site (LonS679A) and is therefore devoid of proteolytic activity, unexpectedly led to complementation of the UV sensitivity and capsule overproduction of a lon deletion mutant. SulA was not degraded by LonS679A, but rather was completely protected by the Lon mutant from degradation by other cellular proteases. We interpret these results to mean that the mutant LonS679A binds but does not degrade Lon substrates, resulting in sequestration of the substrate proteins and interference with their activities, resulting in apparent complementation. Lon that carried a mutation in the consensus ATPase site, either with or without the active site serine, was no longer able to complement a Deltalon mutant. These in vivo results suggest that the pathway of degradation by Lon couples ATP-dependent unfolding with movement of the substrate into protected chambers within Lon, where it is held until degradation proceeds. In the absence of degradation the substrate remains sequestered. Comparison of our results with those from a number of other systems suggest that proteins related to the regulatory portions of energy-dependent proteases act as energy-dependent sequestration proteins. (+info)Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking. (6/706)
SecY and SecE are integral cytoplasmic membrane proteins that form an essential part of the protein translocation machinery in Escherichia coli. Sites of direct contact between these two proteins have been suggested by the allele-specific synthetic phenotypes exhibited by pairwise combinations of prlA and prlG signal sequence suppressor mutations in these genes. We have introduced cysteine residues within the first periplasmic loop of SecY and the second periplasmic loop of SecE, at a specific pair of positions identified by this genetic interaction. The expression of the cysteine mutant pair results in a dominant lethal phenotype that requires the presence of DsbA, which catalyzes the formation of disulfide bonds. A reducible SecY-SecE complex is also observed, demonstrating that these amino acids must be sufficiently proximal to form a disulfide bond. The use of cysteine-scanning mutagenesis enabled a second contact site to be discovered. Together, these two points of contact allow the modeling of a limited region of quaternary structure, establishing the first characterized site of interaction between these two proteins. This study proves that actual points of protein-protein contact can be identified by using synthetic phenotypes. (+info)Specific chromosome alterations in fluconazole-resistant mutants of Candida albicans. (7/706)
The exposure of Candida albicans to fluconazole resulted in the nondisjunction of two specific chromosomes in 17 drug-resistant mutants, each obtained by an independent mutational event. The chromosomal changes occurred at high frequencies and were related to the duration of the drug exposure. The loss of one homologue of chromosome 4 occurred after incubation on a fluconazole medium for 7 days. A second change, the gain of one copy of chromosome 3, was observed after exposure for 35 or 40 days. We found that the mRNA levels of ERG11, CDR1, CDR2, and MDR1, the candidate fluconazole resistance genes, remained either the same or were diminished. The lack of overexpression of putative drug pumps or the drug target indicated that some other mechanism(s) may be operating. The fluconazole resistance phenotype, electrophoretic karyotypes, and transcript levels of mutants were stable after growth for 112 generations in the absence of fluconazole. This is the first report to demonstrate that resistance to fluconazole can be dependent on chromosomal nondisjunction. Furthermore, we suggest that a low-level resistance to fluconazole arising during the early stages of clinical treatment may occur by this mechanism. These results support our earlier hypothesis that changes in C. albicans chromosome number is a common means to control a resource of potentially beneficial genes that are related to important cellular functions. (+info)Escherichia coli gene ydeA encodes a major facilitator pump which exports L-arabinose and isopropyl-beta-D-thiogalactopyranoside. (8/706)
Inactivation of the Escherichia coli gene ydeA, which encodes a member of the major facilitator superfamily, decreased the efflux of L-arabinose, thereby affecting the expression of AraC-regulated genes. In addition, overexpression of ydeA decreased the expression of genes regulated by isopropyl-beta-D-thiogalactopyranoside. (+info)
Improving L-arabinose utilization of pentose fermenting Saccharomyces cerevisiae cells by heterologous expression of L...
Effect of C-Terminal Protein Tags on Pentitol and l-Arabinose Transport by Ambrosiozyma monospora Lat1 and Lat2 Transporters in...
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. | Journal of...
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Co-utilization of L-arabinose and D-xylose by laboratory and industrial Saccharomyces cerevisiae strains | Microbial Cell...
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Characterization of an L-arabinose isomerase from Bacillus coagulans NL01 and its application for D-tagatose production |...
Finding step araH for L-arabinose catabolism in Pseudomonas fluorescens FW300-N1B4
Team:Calgary/Parts/Characterization - 2010.igem.org
araD L-ribulose-5-phosphate 4-epimerase AraD [Escherichia coli str. K-12 substr. MG1655] - Gene - NCBI
Production and Utilization of L-Arabinose in China
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Reconstitution of Sec‐dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG‐dependent manner |...
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THE BINDING OF SUGARS TO THE L-ARABINOSE-BINDING PROTEIN FROM ESCHERICHIA COLI
A novel L-arabinose-responsive regulator found in the rice-blast fungus Pyricularia oryzae (Magnaporthe oryzae) - omicX
Engineering of Pentose Transport in Saccharomyces cerevisiae for Biotechnological Applications - Research database - University...
Expression of Mycobacterium tuberculosis Rv1991c using an arabinose-inducible promoter demonstrates its role as a toxin. -...
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Probable arabinose 5-phosphate isomerase elisa and antibody
Characterization of a monoclonal antibody that recognizes an arabinosylated (1--|6)-beta-D-galactan epitope in plant complex...
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abn2 - Extracellular endo-alpha-(1-|5)-L-arabinanase 2 precursor - Bacillus subtilis (strain 168) - abn2 gene & protein
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Wohl degradation
The classic example is the conversion of glucose to arabinose as shown below. The reaction is named after the German chemist ... Braun, Géza (1940). "D-Arabinose". Organic Syntheses. 20: 14.; Collective Volume, 3, p. 101. Clarke, H. T.; Nagy, S. M. (1940 ...
D-arabinonolactonase
Oxidation of D-arabinose". Journal of Bacteriology. 74 (2): 180-5. PMC 289912. PMID 13475218. Biology portal v t e. ...
Homopolysaccharide
A polysaccharide of arabinose 5. Xylan - A polysaccharide of xylose Champe, Harvey, Ferrier. Biochemistry 4th Edition. 2008. 90 ...
Ribulokinase
Simpson FJ, Wolin MJ, Wood WA (1958). "Degradation of L-arabinose by Aerobacter aerogenes. I. A pathway involving ...
L-ribulose-5-phosphate 4-epimerase
The arabinose system enables the take up the pentose L-arabinose, and then the conversion of intracellular arabinose in three ... Ribulose 5-phosphate 4-epimerase is found on the well studied L-arabinose operon. This operon consists of eight genes araA-araH ... WOLIN MJ, SIMPSON FJ, WOOD WA (1958). "Degradation of L-arabinose by Aerobacter aerogenes. III Identification and properties of ... Schlelf, Robert (December 2000). "Regulation of the L-arabinose operon of Escherichia coli". Trends in Genetics. 16 (12): 559- ...
Catabacter hongkongensis
They produce acid from arabinose, glucose and xylose. They do not produce indole or reduce nitrate. Symptoms of C. ...
Vidarabine
... is an analog of adenosine with the D-ribose replaced with D-arabinose. As you can see from figure 1.1 that it is a ... which contained D-arabinose rather than D-ribose. These compounds led to the synthesis of a new generation, sugar modified ...
Inducer
In the ara operon, arabinose is the inducer. Index inducer or just inducer predictably induce metabolism via a given pathway ...
L-arabinonolactonase
Weimberg R, Doudoroff M (December 1955). "The oxidation of L-arabinose by Pseudomonas saccharophila". The Journal of Biological ...
Arabinosyltransferase
An arabinosyltransferase is a transferase enzyme acting upon arabinose. This enzyme is involved in polymerisation of ... since the arabinose residues occur only in a furanose form. This enzyme has important clinical applications as it is believed ...
Opine
For example, agrocinopine A is a phosphodiester of sucrose and L-arabinose. The name opine comes from octopine, the first opine ... Agrocinopine A is phosphodiester of sucrose and L-Arabinose. Agrocinopine B is the corresponding phosphodiester, in which the ...
Fétizon oxidation
Formation of Tetroses from D-Xylose and L-Arabinose". Acta Chem. Scand. 26: 1709-1710. doi:10.3891/acta.chem.scand.26-1709. ... oxidation of D-xylose and L-arabinose to D-threose and L-erythrose respectively, and oxidation of L-sorbose to afford L-threose ...
Burkholderia thailandensis
It is usually distinguished from B. pseudomallei by its ability to assimilate arabinose. Other differences between these ... Smith MD, Angus BJ, Wuthiekanun V, White NJ (1997). "Arabinose assimilation defines a nonvirulent biotype of Burkholderia ... Smith MD, Angus BJ, Wuthiekanun V, White NJ (1997). "Arabinose assimilation defines a nonvirulent biotype of Burkholderia ... Lertpatanasuwan N, Sermsri K, Petkaseam A, Trakulsomboon S, Thamlikitkul V, Suputtamongkol Y (1999). "Arabinose-positive ...
Gum arabic
Arabinogalactan is a biopolymer consisting of arabinose and galactose monosaccharides. It is a major component of many plant ... Gum arabic is a complex mixture of glycoproteins and polysaccharides predominantly consisting of arabinose and galactose. It is ...
L-arabinitol 4-dehydrogenase
Chiang C, Knight SG (1961). "L-Arabinose metabolism by cell-free extracts of Penicillium chrysogenum". Biochim. Biophys. Acta. ...
Cytarabine
Cytosine arabinoside combines a cytosine base with an arabinose sugar. It is an antimetabolic agent with the chemical name of 1 ...
UTP-xylose-1-phosphate uridylyltransferase
"ENZYMATIC SYNTHESIS OF URIDINE DIPHOSPHATE XYLOSE AND URIDINE DIPHOSPHATE ARABINOSE". Proc. Natl. Acad. Sci. U.S.A. 42 (6): 333 ...
Arabinogalactan
Both the arabinose and galactose exist solely in the furanose configuration. The galactan portion of microbial arabinogalactan ... Arabinogalactan is a biopolymer consisting of arabinose and galactose monosaccharides. Two classes of arabinogalactans are ...
Ruff degradation
In 1898, Otto Ruff published his work on the transformation of D-Glucose to D-Arabinose later called the Ruff degradation. In ... thus forming D-Arabinose. Ruff degradation shortens an aldose chain by removing one carbon v t e. ... this reaction, D-Glucose is converted to D-Arabinose . In this reaction, the terminal aldehyde group is converted to a ...
Regulator gene
ISBN 978-0-7167-4939-4. Casadaban, Malcolm J. (1976-07-05). "Regulation of the regulatory gene for the arabinose pathway, araC ...
Repressor
The L-arabinose operon houses genes coding for arabinose-digesting enzymes. These function to break down arabinose as an ... In the presence of both Arabinose and araC, araC binds with the arabinose and acts as an activator. This conformational change ... In the absence of Arabinose but presence of araC, araC regions form dimers, and bind to bring ara02 and araI1 domains closer by ... In the absence of Arabinose and araC (repressor), loop formation is not initiated and structural gene expression will be lower ...
UDP-glucuronic acid dehydrogenase
A key enzyme for lipid A modification with 4-amino-4-deoxy-L-arabinose and polymyxin resistance". Biochemistry. 43 (42): 13370- ... Identification and function oF UDP-4-deoxy-4-formamido-L-arabinose". The Journal of Biological Chemistry. 280 (14): 14154-67. ... involved in 4-amino-4-deoxy-L-arabinose biosynthesis". The Journal of Biological Chemistry. 280 (24): 23000-8. doi:10.1074/jbc. ... UDP-4-amino-4-deoxy-L-arabinose formyltransferase). Breazeale SD, Ribeiro AA, McClerren AL, Raetz CR (April 2005). "A ...
Ribulose
... sugars are composed in the pentose phosphate pathway from arabinose. They are important in the formation of many ... "Characterization of an L-Arabinose Isomerase from Bacillus velezensis and Its Application for L-Ribulose and L-Ribose ...
Petasis reaction
Vinylboronic acid is first coupled with L-arabinose 1 and Bis(4-methoxyphenyl)methanamine 2 to form an stereochemically-defined ... Petasis used this reaction to prepare Boc-protected mannosamine from D-arabinose. With chiral amine nucleophile Generally ...
Zymomonas mobilis
Wild-type Z. mobilis cannot ferment C5 sugars like xylose and arabinose which are important components of lignocellulosic ... Deanda, K; Zhang, M; Eddy, C; Picataggio, S (1996). "Development of an arabinose-fermenting Zymomonas mobilis strain by ... USA has made significant contributions in expanding its substrate range to include C5 sugars like xylose and arabinose., Acetic ...
SAMHD1
... has been shown to be able to hydrolyze arabinose 5'-triphosphates. SAMHD1 has been shown to be a biomarker and influence ... arabinose C (ara-C; cytarabine) responsiveness. Viral protein x (Vpx) has been proposed to be potential therapy to improve ...
Arabitol
It can be formed by the reduction of either arabinose or lyxose. Some organic acid tests check for the presence of D-arabitol, ...
Xylose isomerase
This bacterium was mistakenly classified as a L. plantarum, which normally grows on the sugar L-arabinose, and rarely grown on ... Some examples include: D-ribose, L-arabinose, L-rhamnose, and D-allose. Conversion of glucose to fructose by xylose isomerase ... and L-arabinose. Twenty hexoses and nine pentoses, including xylulose, were considered to be "rare sugars". Hence D-xylose ...
Cellulosic ethanol
Karhumaa K, Wiedemann B, Hahn-Hagerdal B, Boles E, Gorwa-Grauslund MF (2006) Co-utilization of L-arabinose and D-xylose by ... Becker J, Boles E (2003). "A modified Saccharomyces cerevisiae strain that consumes L-Arabinose and produces ethanol". Appl ... and arabinose, and even both together. Yeast cells are especially attractive for cellulosic ethanol processes because they have ... a significant amount of xylose and arabinose (five-carbon sugars derived from the hemicellulose portion of the lignocellulose) ...
Tartronic acid
Hall A. N.; Kulka D.; Walker T. K. (1955). "Formation of arabinose, ribulose and tartronic acid from 2-keto-d-gluconic acid". ...
D-arabinose (CHEBI:17108)
D-arabinose 5-phosphate (CHEBI:79058) has functional parent D-arabinose (CHEBI:17108). aldehydo-D-arabinose (CHEBI:46983) is a ... D-arabinose (CHEBI:17108) has role Saccharomyces cerevisiae metabolite (CHEBI:75772) D-arabinose (CHEBI:17108) is a arabinose ( ... D-arabinofuranose (CHEBI:79054) is a D-arabinose (CHEBI:17108). D-arabinopyranose (CHEBI:46994) is a D-arabinose (CHEBI:17108) ... CHEBI:17108 - D-arabinose. Main. ChEBI Ontology. Automatic Xrefs. Reactions. Pathways. Models. ...
Arabinose - Wikipedia
However, L-arabinose is in fact more common than D-arabinose in nature and is found in nature as a component of biopolymers ... Arabinose gets its name from gum arabic, from which it was first isolated. In synthetic biology, arabinose is often used as a ... The operon directs the catabolism of arabinose in E. coli, and it is dynamically activated in the presence of arabinose and the ... The L-arabinose operon, also known as the araBAD operon, has been the subject of much biomolecular research. ...
aldehydo-D-arabinose 5-phosphate (CHEBI:16241)
... is a D-arabinose 5-phosphate (CHEBI:79058) aldehydo-D-arabinose 5-phosphate ( ... aldehydo-D-arabinose 5-phosphate(2−) (CHEBI:57693) is conjugate base of aldehydo-D-arabinose 5-phosphate (CHEBI:16241). ... CHEBI:16241 - aldehydo-D-arabinose 5-phosphate. Main. ChEBI Ontology. Automatic Xrefs. Reactions. Pathways. Models. ... The 5-phospho derivative of D-arabinose. It is an intermediate in the synthesis of lipopolysaccharides.. ...
Arabinose isomerase - Wikipedia
Other names in common use include D-arabinose(L-fucose) isomerase, D-arabinose isomerase, L-fucose isomerase, and D-arabinose ... In enzymology, an arabinose isomerase (EC 5.3.1.3) is an enzyme that catalyzes the chemical reaction D-arabinose ⇌ {\ ... Cohen SS (1953). "Studies on D-ribulose and its enzymatic conversion to D-arabinose". J. Biol. Chem. 201 (1): 71-84. PMID ... The systematic name of this enzyme class is D-arabinose aldose-ketose-isomerase. ...
Transport and metabolism of glucose and arabinose in Bifidobacterium breve. - PubMed - NCBI
Arabinose uptake in B. breve was not directly dependent on phosphorylation or any other energy-linked form of transport but may ... 2-DC had no effect on arabinose uptake, but NaF was stimulatory. High levels of phosphorylation of glucose and 2-DC by PEP and ... Studies using D-[U-14C]-labelled arabinose showed that it was fermented to pyruvate, formate, lactate and acetate, whereas the ... Transport and metabolism of glucose and arabinose in Bifidobacterium breve.. Degnan BA1, Macfarlane GT. ...
Production and Utilization of L-Arabinose in China
L-arabinose is mainly produced by chemical or enzymatic hydrolysis of hemicellulose. The taste of L-arabinose is quite similar ... However, L-arabinose has not been widely used in functional foods due to high price and lack of publicity and guidance. A ... As a functional additive, L-arabinose can be used in medical and pharmaceutical applications for the treatment of diseases such ... L-arabinose is a newly developed low-caloric monosaccharide, which has many biomedical and health effects, especially ...
RCSB PDB - Protein Feature View
- Arabinose 5-phosphate isomerase - Q60AU8 (Q60AU8 METCA)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
SPECTRUM L-Arabinose, 1kg, CAS 5328-37-0, Poly - 46AG33|AR110-1KG - Grainger
87-72-9 - L-(+)-Arabinose, 99% - A11921 - Alfa Aesar
L-(+)-Arabinose is used to identify and differentiate and characterize pentose sugar isomerase. It is also used as the carbon ... A Link between Arabinose Utilization and Oxalotrophy in Bradyrhizobium japonicum. Appl. Environ. Microbiol. 2014, 80 (7), 2094- ... L-Arabinose Binding, Isomerization, and Epimerization by D-Xylose Isomerase: X-Ray/Neutron Crystallographic and Molecular ...
Mechanism of ultraviolet light induced catabolite repression of L-arabinose isomerase (Journal Article) | ETDEWEB
ETDEWEB / Search Results / Mechanism of ultraviolet light induced catabolite repression of L-arabinose isomerase ... Bhatnagar, D, and Bhattacharya, A K. Mechanism of ultraviolet light induced catabolite repression of L-arabinose isomerase. ... Bhatnagar, D, & Bhattacharya, A K. Mechanism of ultraviolet light induced catabolite repression of L-arabinose isomerase. ... title = {Mechanism of ultraviolet light induced catabolite repression of L-arabinose isomerase}. author = {Bhatnagar, D, and ...
araC - Arabinose operon regulatory protein - Escherichia coli (strain K12) - araC gene & protein
In the presence of arabinose, activates the expression of the araBAD, araE, araFGH and araJ promoters (PubMed:4362626, PubMed: ... 328165, PubMed:6251457, PubMed:2962192, PubMed:6319708, PubMed:2231717, PubMed:1447222). In the absence of arabinose, ... Transcription factor that regulates the expression of several genes involved in the transport and metabolism of L-arabinose ( ... Arabinose operon regulatory proteinAdd BLAST. 292. Proteomic databases. PaxDb, a database of protein abundance averages across ...
Engineering Pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose. - PubMed - NCBI
Utilization of d-xylose and l-arabinose may proceed via (partly) shared pathways. Indications were found that l-arabinose can ... glucose and arabinose (B); or glucose, xylose, and arabinose (C). Data points are the averages of the results of duplicate ... Engineering Pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose.. Meijnen JP1, de Winde JH, ... Surprisingly, without any further engineering, the evolved D-xylose-utilizing strain metabolized l-arabinose as efficiently as ...
CAS No.87-72-9,L(+)-Arabinose Suppliers
Arabinose) for price inquiry. where to buy 87-72-9(L(+)-Arabinose).Also offer free database of 87-72-9(L(+)-Arabinose) ... Product Name: L(+)-Arabinose Synonyms: BETA-L-ARABINOPYRANOSE;BETA-L-(+)-ARABINOSE;L-(+)-ARABINOSE;Arabinose(L);L-(+)-ARABINOSE ... L-(+)-Arabinose CAS No. 87-72-9 Brand: Orchid Chemical Specifications Name L-Arabinose Synonyms L-(+)-Arabinose; L- ... Best price arabinose for hot sale/cas87-72-9 1.Basic information: 1. CAS NO.: 87-72-9 2. Appearance: White crystalline powder 3 ...
CAS No.5328-37-0,L-Arabinose Suppliers
where to buy 5328-37-0(L-Arabinose).Also offer free database of 5328-37-0(L-Arabinose) including MSDS sheet(poisoning, toxicity ... food grade Sweetener aldehydo-L-arabinose cas:5328-37-0 Product name: food grade Sweetener aldehydo-L-arabinose cas:5328-37-0 ... L-Arabinose 1) Quick Response Within 12 hours; 2) Quality Guarantee: All products are strictly tested by our QC, confirmed by ... Product Name L-Arabinose Appearance white crystalline powder CAS NO. 5328-37-0 Molecular Formula C5H10O5 Molecular Weight ...
At5g44480 - Putative UDP-arabinose 4-epimerase 4 - Arabidopsis thaliana (Mouse-ear cress) - At5g44480 gene & protein
UDP-arabinose 4-epimerase 1 (MUR4), Probable UDP-arabinose 4-epimerase 3 (At4g20460), Putative UDP-arabinose 4-epimerase 4 ( ... UDP-L-arabinose biosynthesis. This protein is involved in step 1 of the subpathway that synthesizes UDP-L-arabinose from UDP- ... At5g44480), Putative UDP-arabinose 4-epimerase 2 (At2g34850). This subpathway is part of the pathway UDP-L-arabinose ... Putative UDP-arabinose 4-epimerase 4Add BLAST. 436. Proteomic databases. PaxDb, a database of protein abundance averages across ...
CiteSeerX - Cloning, Functional Analysis, and Transcriptional Regulation of the Bacillus subtilis araE Gene Involved in L...
These observations are consistent with a model according to which in the absence of L-arabinose, AraR binds to a site(s) within ... Therefore, we assume that araE encodes a permease involved in L-arabinose transport into the cell. The araE promoter region ... Transcriptional studies demonstrated that the expression from the araE promoter is induced by L-arabinose, repressed by glucose ... termed araE by analogy to the Escherichia coli L-arabinose permease gene. Here, we report the cloning and sequencing of the ...
View topic - Carbo Sep Coregel 87P and molecule of arabinose & mannose?
So, arabinose would elute ~16 min from the 87P column, but 24min on the CHO 682 column.. Have a good day ... The column Carbo Sep Coregel 87P will be a column appropriated in order to analyse the molecule of arabinose and mannose ?. ... Re: Carbo Sep Coregel 87P and molecule of arabinose & mannos. by flerfol » Tue 22 Dec 2015 10:34 ... The 87P would only provide ~30 seconds between peaks of Mannose and Arabinose. The CHO 682 column (PN: CHO-99-9854) would give ...
Use of cephalexin-aztreonam-arabinose agar for selective isolation of Enterococcus faecium. | Journal of Clinical Microbiology
Use of cephalexin-aztreonam-arabinose agar for selective isolation of Enterococcus faecium.. M Ford, J D Perry, F K Gould ... Cephalexin-aztreonam-arabinose agar (CAA), a new selective agar, was examined in comparison with nalidixic acid-colistin agar ... Use of cephalexin-aztreonam-arabinose agar for selective isolation of Enterococcus faecium. ... Use of cephalexin-aztreonam-arabinose agar for selective isolation of Enterococcus faecium. ...
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. | Journal of...
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.. L M Guzman, D Belin ... phoA expression can be modulated over a wide range of inducer (arabinose) concentrations and reduced to extremely low levels by ... We have constructed a series of plasmid vectors (pBAD vectors) containing the PBAD promoter of the araBAD (arabinose) operon ... Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. ...
RCSB PDB
- 1APB: A PRO TO GLY MUTATION IN THE HINGE OF THE ARABINOSE-BINDING PROTEIN ENHANCES BINDING AND ALTERS...
A Pro to Gly mutation in the hinge of the arabinose-binding protein enhances binding and alters specificity. Sugar-binding and ... L-arabinose binding protein-like L-arabinose-binding protein Escherichia coli [TaxId: 562] ... A PRO TO GLY MUTATION IN THE HINGE OF THE ARABINOSE-BINDING PROTEIN ENHANCES BINDING AND ALTERS SPECIFICITY: SUGAR-BINDING AND ...
Effects of a Dietary Supplement Containing L-Arabinose and Trivalent Patented Food-source of Chromium on Blood Glucose and...
Effects of a Dietary Supplement Containing L-Arabinose and Trivalent Patented Food-source of Chromium on Blood Glucose and ... A combination of l-arabinose and chromium lowers circulating glucose and insulin levels after an acute oral sucrose challenge. ... Insulin (L-Arabinose). The safety and scientific validity of this study is the responsibility of the study sponsor and ...
Method of Producing Xylitol Using Hydrolysate Containing Xylose and Arabinose Prepared from Byproduct of Tropical Fruit Biomass...
Disclosed is a method of producing xylitol using a hydrolysate containing xylose and arabinose prepared from byproducts of ... Arabinose. Xylose. Arabinose. Xylose. Arabinose. Yield. Purity. Yield. Purity. Yield. Purity. Yield. Purity. Yield. Purity. ... Method of Producing Xylitol Using Hydrolysate Containing Xylose and Arabinose Prepared from Byproduct of Tropical Fruit Biomass ... As a result, the fraction with xylose in high content and the fraction having high arabinose content can be obtained. The ...
Misdiagnosis of Cytosine arabinose syndrome - RightDiagnosis.com
Misdiagnosis of Cytosine arabinose syndrome including hidden diseases, diagnosis mistakes, alternative diagnoses, differential ... When checking for a misdiagnosis of Cytosine arabinose syndrome or confirming a diagnosis of Cytosine arabinose syndrome, it is ... Cytosine arabinose syndrome: Medical Mistakes. Related medical mistakes may include: *Cancer Health Mistakes: *Cancer (All) -- ... Common Misdiagnoses and Cytosine arabinose syndrome. Spitz nevi misdiagnosed as dangerous melanoma skin cancer: One possible ...
Single Cell Kinetics of Phenotypic Switching in the Arabinose Utilization System of E. coli
Because the system is regulated via the internal arabinose level sensed by AraC, internal arabinose must be rapidly depleted by ... The model also predicts that mutations which disrupt the positive feedback of internal arabinose on the production of arabinose ... which is part of the arabinose regulon and has been annotated as a possible arabinose efflux protein, is responsible for rapid ... Here, we analyze the switching kinetics of a paradigmatic inducible system, the arabinose utilization system in E. coli. Using ...
Bioconversion of D-galactose to D-tagatose: continuous packed bed reaction with an immobilized thermostable L-arabinose...
L-arabinose isomerase from Thermoanaerobacter mathranii (TMAI) was recombinantly overexpressed and immobilized in calcium ... The continuous enzymatic conversion of D-galactose to D-tagatose with an immobilized thermostable L-arabinose isomerase in ... Bioconversion of D-galactose to D-tagatose: continuous packed bed reaction with an immobilized thermostable L-arabinose ...
THE BINDING OF SUGARS TO THE L-ARABINOSE-BINDING PROTEIN FROM ESCHERICHIA COLI
... electron density allowed for a model for L-arabinose binding to the L-arabinose binding protein to be proposed. The L-arabinose ... The sugar-binding site of the L-arabinose binding protein, an essential component of the high-affinity L-arabinose uptake ... THE BINDING OF SUGARS TO THE L-ARABINOSE-BINDING PROTEIN FROM ESCHERICHIA COLI. ... The observation that the original structure might have been solved with bound L-arabinose necessitated the synthesis of the ...
Engineering of Saccharomyces cerevisiae for Efficient Anaerobic Alcoholic Fermentation of l-Arabinose | Applied and...
... plantarum arabinose isomerase for arabinose (28 mM) (11) may have contributed to the decreasing arabinose consumption rates. In ... Expression of the L. plantarum arabinose pathway in S. cerevisiae.In this work, araA (l-arabinose isomerase), araB (l- ... Production of ethanol from l-arabinose by Saccharomyces cerevisiae containing a fungal l-arabinose pathway. FEMS Yeast Res. 3: ... Although the arabinose in the anaerobic cultures was completely consumed, a decreasing arabinose consumption rate over time at ...
D-Arabinose | GoldBio
D-Arabinose is a reducing sugar useful as a substrate in analysis of arabinose isomerases and synthesis of D-erythroascorbic ... D-arabinose is converted to D-ribulose by an arabinose isomerase enzyme, which can then be phosphorylated and enter the pentose ... As opposed to most other sugars, D-arabinose, featured here, is actually less commonly found in nature than L-arabinose. ... D-Arabinose is a five-carbon reducing sugar. It is a constituent of arabinogalactans that make up the cell wall of mycobacteria ...
The oxidation of L-arabinose by Pseudomonas saccharophila. - Semantic Scholar
Preliminary studies revealed that the oxidation of n-arabinose by this organism is strikingly different from that of glucose. ... The oxidation of L-arabinose by Pseudomonas saccharophila.. @article{Weimberg1955TheOO, title={The oxidation of L-arabinose by ... trifolii characterised and applied for bioconversion of l-arabinose to l-arabonate with Saccharomyces cerevisiae. *Niina Aro- ... Enzymatic Characterization of 17 L-arabinose Negative Mutants of Escherichia Coli. *ELLIS ENGLESBEIRG ...
DIGITAL.CSIC: One-pot cascade reactions using fructose-6-phosphate aldolase: Efficient synthesis of D-arabinose 5-phosphate, D...
IsomeraseOperonUtilizationEnzymeGlucose and arabinoseSucraseMonosaccharideGalactoseUptakeFunction of Arabinose concentrationGenePresence of L-arabinoseOxidation of L-arabinoseCatabolismFungal arabinose pathwaySucroseProteinPathwayXylose and arabinoseSynthesisAbstractFood AdditiveSweetenerHydrolysisMannoseCrystallineAraCBiomassSubstrateAnaerobic growthC5H10O5MutantsMetabolicSugar BlockerAbsorptionPectinSugarsFermentationConcentrationDegradationConcentrationsBindsInducerCytosineDisplaystyleConvertsHemicelluloseIsomerizationPolysaccharideTrehalosePromoterYeast
Isomerase24
- In enzymology, an arabinose isomerase (EC 5.3.1.3) is an enzyme that catalyzes the chemical reaction D-arabinose ⇌ {\displaystyle \rightleftharpoons } D-ribulose Hence, this enzyme has one substrate, D-arabinose, and one product, D-ribulose. (wikipedia.org)
- The systematic name of this enzyme class is D-arabinose aldose-ketose-isomerase. (wikipedia.org)
- Other names in common use include D-arabinose(L-fucose) isomerase, D-arabinose isomerase, L-fucose isomerase, and D-arabinose ketol-isomerase. (wikipedia.org)
- L-(+)-Arabinose is used to identify and differentiate and characterize pentose sugar isomerase. (alfa.com)
- Kovalevsky, A. L-Arabinose Binding, Isomerization, and Epimerization by D-Xylose Isomerase: X-Ray/Neutron Crystallographic and Molecular Simulation Study. (alfa.com)
- An attempt has been made to find out how U.V. irradiation of E.coli B/r cells causes catabolite repression to inhibit L-arabinose isomerase synthesis. (osti.gov)
- Bhatnagar, D, and Bhattacharya, A K. Mechanism of ultraviolet light induced catabolite repression of L-arabinose isomerase. (osti.gov)
- misc{etde_6096134, title = {Mechanism of ultraviolet light induced catabolite repression of L-arabinose isomerase} author = {Bhatnagar, D, and Bhattacharya, A K} abstractNote = {An attempt has been made to find out how U.V. irradiation of E.coli B/r cells causes catabolite repression to inhibit L-arabinose isomerase synthesis. (osti.gov)
- Bioconversion of D-galactose to D-tagatose: continuous packed bed reaction with an immobilized thermostable L-arabinose isomerase and efficient purification by selective microbial degradation. (sigmaaldrich.com)
- The continuous enzymatic conversion of D-galactose to D-tagatose with an immobilized thermostable L-arabinose isomerase in packed-bed reactor and a novel method for D-tagatose purification were studied. (sigmaaldrich.com)
- L-arabinose isomerase from Thermoanaerobacter mathranii (TMAI) was recombinantly overexpressed and immobilized in calcium alginate. (sigmaaldrich.com)
- D-arabinose is converted to D-ribulose by an arabinose isomerase enzyme, which can then be phosphorylated and enter the pentose phosphate pathway. (goldbio.com)
- L-arabinose isomerase (AI) is a crucial catalyst for the biotransformation of D-galactose to D-tagatose. (springer.com)
- Another method is the enzymatic process that mainly depends on L-arabinose isomerase (AI, EC 5.3.1.4). (springer.com)
- Below are the list of possible Probable arabinose 5-phosphate isomerase products. (mybiosource.com)
- Also known as Probable arabinose 5-phosphate isomerase (API). (mybiosource.com)
- Gene araA encoding an l -arabinose isomerase (AraA) from the hyperthermophile, Thermotoga neapolitana 5068 was cloned, sequenced, and expressed in Escherichia coli . (oup.com)
- The deduced amino acid sequence has 94.8% identical amino acids compared with the residues in a putative l -arabinose isomerase of Thermotoga maritima . (oup.com)
- This chapter describes the assay method, purification procedure, and properties of L-arabinose isomerase. (sacredheartrs.org)
- Geobacillus thermodenitrificans, with a double-site mutation in l-arabinose isomerase, produced 95 g l-ribulose l−1 from g l-arabinose l−1 under. (sacredheartrs.org)
- In enzymology, a L-arabinose isomerase (EC ) is an enzyme that catalyzes the chemical reaction. (sacredheartrs.org)
- In a previous work (Paveia, 1986) the structural genes for L-arabinose utilization araA, araB and araD - coding for L-arabinose isomerase, L-ribulokinase and L-ribulose 5-phosphate 4-epimerase, respectively and mutations in a presumptive regulatory gene araC were mapped. (core.ac.uk)
- The characterization of Ara'C mutants by L-arabinose isomerase activity revealed that this activity was reduced in the presence of L-arabinose and repressed by glucose. (core.ac.uk)
- The products of the structural genes exhibited similarity in aminoacid composition and protein sizes to the enzymes L-arabinose isomerase, L-ribulokinase and L-ribulose 5-phosphate 4-epimerase of E.coli and S.thyphimurium. (core.ac.uk)
Operon10
- The L-arabinose operon, also known as the araBAD operon, has been the subject of much biomolecular research. (wikipedia.org)
- The operon directs the catabolism of arabinose in E. coli, and it is dynamically activated in the presence of arabinose and the absence of glucose. (wikipedia.org)
- 3279415 ). In the absence of arabinose, AraC binds to the araO2 and araI1 half-sites in the promoter region of the araBAD operon, leading to the formation of a DNA loop that blocks access of RNA polymerase to the promoter. (uniprot.org)
- We have constructed a series of plasmid vectors (pBAD vectors) containing the PBAD promoter of the araBAD (arabinose) operon and the gene encoding the positive and negative regulator of this promoter, araC. (asm.org)
- Using time-lapse fluorescence microscopy of microcolonies in a microfluidic chamber, which permits sudden up-and down-shifts in the inducer arabinose, we characterize the single-cell gene expression dynamics of the araBAD operon responsible for arabinose degradation. (uni-muenchen.de)
- High-affinity L-arabinose transport operon. (semanticscholar.org)
- The nucleotide sequence of the 'high-affinity' L-arabinose transport operon has been determined 3' from the regulatory region and found to contain three open reading frames designated araF, araG and araH. (semanticscholar.org)
- The L-arabinose operon of the model bacterium Escherichia coli has been a focus for research in molecular biology for over 40 years, and has been investigated extensively at the genetic , biochemical , physiological , and biophysical levels. (wikidoc.org)
- In this thesis, we propose a Boolean model of a similar nature for the arabinose operon. (clemson.edu)
- Accumulation of [ 3 H]arabinose in mutants and wild-type was measured, and the results suggested that this operon is necessary for arabinose transport. (microbiologyresearch.org)
Utilization8
- Pessi, G. A Link between Arabinose Utilization and Oxalotrophy in Bradyrhizobium japonicum. (alfa.com)
- Engineering Pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose. (nih.gov)
- Utilization of d -xylose and l -arabinose may proceed via (partly) shared pathways. (nih.gov)
- Here, we analyze the switching kinetics of a paradigmatic inducible system, the arabinose utilization system in E. coli. (uni-muenchen.de)
- This fermentation was achieved by combining the expression of the structural genes for the l -arabinose utilization pathway of Lactobacillus plantarum , the overexpression of the S. cerevisiae genes encoding the enzymes of the nonoxidative pentose phosphate pathway, and extensive evolutionary engineering. (asm.org)
- The aim os this work was to study the genes involved in L-arabinose utilization of Bacillus subtilis. (core.ac.uk)
- A first approach in this study, the isolation of constitutive mutants for L-arabinose utilization (Ara'c) and mapping of ara'c mutations, showed that they are located in the locus araC between the cys B and his A markers on the B.subtilis chromosome. (core.ac.uk)
- Although l-arabinose is a highlighted pentose that has been identified as next-generation biomass, arabinose fermentation has not yet undergone extensive development for industrial utilization. (elsevier.com)
Enzyme13
- citation needed] Originally commercialized as a sweetener, arabinose is an inhibitor of sucrase, the enzyme that breaks down sucrose into glucose and fructose in the small intestine. (wikipedia.org)
- Yields of Larabinose recovery for the crude extract and the commercial enzyme were 17.04 % and 2.78 %, respectively, based on the reported average content of L-arabinose in mesquite gum. (redalyc.org)
- The thermophilic enzyme had a maximum activity of l -arabinose isomerization and d -galactose isomerization at 85°C, and required divalent cations such as Co 2+ and Mn 2+ for its activity and thermostability. (oup.com)
- The apparent K m values of the enzyme for l -arabinose and d -galactose were 116 mM ( v max , 119 μmol min −1 mg −1 ) and 250 mM ( v max , 14.3 μmol min −1 mg −1 ), respectively, that were determined in the presence of both 1 mM Co 2+ and 1 mM Mn 2+ . (oup.com)
- Yields of L-arabinose recovery for the crude extract and the commercial enzyme were 17.04 % and 2.78 %, respectively, based on the reported average content of L-arabinose in mesquite gum. (scielo.org.mx)
- L-arabinose - inhibits enzyme sucrase that digests sucrose, thus suppressing glycemic index and caloric impact of sugary food. (alphawellness.com.sg)
- L-arabinose, derived from non-GMO corn cob, inhibits enzyme sucrase that digests sucrose, thus reducing sugar uptake. (alphawellness.com.sg)
- 1.L-arabinose can be used as a sucrose enzyme inhibitor to lower the Glycaemic Index(GI) of sucrose containing products. (cbaextract.com)
- The most representative feature of L-Arabinose is that it can selectively affect the sucrose enzyme which influences the sucrose digestion indisaccharide-hydrolysing enzymes in small intestine, and so Arabinose can well stop the absorption of sucrose in body. (cbaextract.com)
- When consumed, L-arabinose selectively inhibits the sucrase enzyme in the small intestine. (techconnect.org)
- During the period that the sucrase enzyme is inhibited by L-arabinose, the splitting of sucrose is delayed or even prevented, and the release of glucose and fructose to the blood is delayed. (techconnect.org)
- This activity of L-Arabinose is selective to the sucrase enzyme. (techconnect.org)
- Enzyme activity assays showed that the Weimberg pathway was active in cells grown, not only on xylose but also on arabinose. (mdpi.com)
Glucose and arabinose2
Sucrase4
- L-arabinose is a newly developed low-caloric monosaccharide, which has many biomedical and health effects, especially intestinal sucrase inhibition effect. (scirp.org)
- 1.Inhibit the metabolism and absorption of sucrose, L-arabinose is the most representative of the physiological effect of the selective influence of intestinal sucrase, thereby inhibiting the absorption of sucrose. (dealchemical.com)
- L Arabinose appears to selectively inhibit the sucrase activity in the small intestine which can control the increase of blood sugar caused by intake of sugar. (theingredienthouse.com)
- L-arabinose is a kind of aldopentoses, it's a low-calurie sweetener, it inhibits the sucrase activity and prevents increasing of blood glucose, therefore, it can prevent fat accumulation and against high blood glucose disease. (cbaextract.com)
Monosaccharide4
- Arabinose is an aldopentose - a monosaccharide containing five carbon atoms, and including an aldehyde (CHO) functional group. (wikipedia.org)
- Combined with other monosaccharide unions, Arabinose can be found in sol, hemicellulose, pectic acid, bacterium polysaccharide and certain sugar glucoside by mixed polysaccharide form. (dealchemical.com)
- They encode the metabolic enzymes for breaking down the monosaccharide sugar arabinose into D-xylulose-5-phosphate , which is then metabolised via the pentose phosphate pathway . (wikidoc.org)
- L Arabinose is a monosaccharide containing 5 carbon atoms. (theingredienthouse.com)
Galactose6
- The location of a C6 substituent by difference Fourier analysis of crystals soaked in solutions of the C6 analog D-galactose and a fit of an L-arabinose molecule to the "extraneous" electron density allowed for a model for L-arabinose binding to the L-arabinose binding protein to be proposed. (rice.edu)
- l-Arabinose/d-galactose 1-dehydrogenase of Rhizobium leguminosarum bv. (semanticscholar.org)
- The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products. (megazyme.com)
- Pectin, PECTIN It consists of methylated galacturonic acid, galactose & arabinose. (expertsmind.com)
- Transcription of this region was measured by β -galactosidase assays of Tn 5 -B20 fusions, and shown to be strongly inducible by arabinose, and moderately so by galactose and seed exudate. (microbiologyresearch.org)
- Although catabolite repression of the arabinose genes by succinate or glucose was not detected at the level of transcription, both glucose and galactose were found to inhibit accumulation of arabinose when present in excess. (microbiologyresearch.org)
Uptake6
- 2-DC had no effect on arabinose uptake, but NaF was stimulatory. (nih.gov)
- Arabinose uptake in B. breve was not directly dependent on phosphorylation or any other energy-linked form of transport but may be assimilated by glucose-dependent facilitated diffusion. (nih.gov)
- The model also predicts that mutations which disrupt the positive feedback of internal arabinose on the production of arabinose uptake proteins change the heterogeneous on-switching behavior into a homogeneous, graded response. (uni-muenchen.de)
- The sugar-binding site of the L-arabinose binding protein, an essential component of the high-affinity L-arabinose uptake system in Escherichia coli, is located deep in a cleft formed by the two domains of the protein. (rice.edu)
- To determine if glucose was also taken up by the arabinose transport proteins, [ 14 C]glucose uptake rates were measured in wild-type and arabinose mutant strains. (microbiologyresearch.org)
- No differences in glucose uptake rates were detected between wild-type and arabinose catabolism mutant strains, indicating that excess glucose did not compete with arabinose for transport by the same system. (microbiologyresearch.org)
Function of Arabinose concentration2
Gene3
- Moreover, the results suggest that other loci are required for arabinose catabolism, including a gene encoding arabinose dehydrogenase. (microbiologyresearch.org)
- In this study, we integrated a heterologous fungal arabinose pathway with a deletion of PHO13 phosphatase gene. (elsevier.com)
- PHO13 deletion increased arabinose consumption rate and specific ethanol productivity under aerobic conditions and consequently depleted sedoheptulose by activation of the TAL1 gene. (elsevier.com)
Presence of L-arabinose1
- Figure 2 (a) shows the presence of L-arabinose in corn cob hemicellulose: the backbone is β-1,4-conjugated D-xylan, which can be combined with the hemiacetal hydroxyl of L-arabinose in the side chain at both the 2 and 3 positions of the xylose residue. (scirp.org)
Oxidation of L-arabinose2
- The oxidation of L-arabinose by Pseudomonas saccharophila. (semanticscholar.org)
- In this work, kinetics of selective oxidation of L-arabinose to arabinonic acid over Au/Al 2 O 3 was studied using data from experiments in a semibatch shaker reactor under pH-controlled conditions at atmospheric pressure. (abo.fi)
Catabolism1
- In this work, a previously undeleted region of the megaplasmid pSymB was identified as encoding genes necessary for arabinose catabolism, by Tn 5 -B20 random mutagenesis and subsequent complementation. (microbiologyresearch.org)
Fungal arabinose pathway1
- The rarity of ethanolic arabinose fermentation may be due to a redox imbalance in the fungal arabinose pathway as a result of the use of NADPH for the reductive reactions and the production of NADH in the oxidation reactions ( 6 , 36 ) (Fig. 1 ). (asm.org)
Sucrose9
- The taste of L-arabinose is quite similar to that of sucrose, with approximately 50% of the sweetness. (scirp.org)
- The sweetness of L-arabinose is about half of sucrose. (scirp.org)
- A combination of l-arabinose and chromium lowers circulating glucose and insulin levels after an acute oral sucrose challenge. (clinicaltrials.gov)
- In dinitrophenol-treated tissues, in which all permeability processes are irreversibly damaged and in which sucrose and pentoses penetrate into most of the cell water, the entry rate of pentoses and sucrose is initially similar but subsequently D -xylose and L -arabinose penetrate more rapidly than their corresponding optical isomers. (rupress.org)
- 3.Prevention of constipation, promote the growth of bifidobacteria, intake arabinose added 5% sucrose also can promote bifidobacteria (Bifidobacterium) growth. (dealchemical.com)
- 1. Arabinose can take metabolic role to sucrose in the intestines, and can stop the conversion. (cbaextract.com)
- Reducing glycemic response in food products with L-Arabinose L-Arabinose can play an important role in the management of blood glucose and insulin levels related to sucrose intake. (techconnect.org)
- The addition of L-Arabinose to a sucrose containing diet maintains all advantages of sucrose while strongly reducing the impact of sucrose consumption on the human body. (techconnect.org)
- Depending on the amount of L-arabinose in the small intestine, the digestion of sucrose is delayed and/or (partially) prevented. (techconnect.org)
Protein9
- In synthetic biology, arabinose is often used as a one-way or reversible switch for protein expression under the Pbad promoter in E. coli. (wikipedia.org)
- We explored whether the poorly characterized membrane protein AraJ, which is part of the arabinose regulon and has been annotated as a possible arabinose efflux protein, is responsible for rapid depletion. (uni-muenchen.de)
- The observation that the original structure might have been solved with bound L-arabinose necessitated the synthesis of the heavy-atom analog, its structure consistent with the sugar-binding specificity of the protein. (rice.edu)
- Small angle X-ray scattering experiments indicate that the L-arabinose-binding protein undergoes a substantial conformational change upon the binding of substrate: the radius of gyration of the protein molecule decreases by (TURN)1 A with the addition of L-arabinose. (rice.edu)
- If arabinose is absent, the AraC protein assumes a different conformation. (wikidoc.org)
- L-arabinose ABC transporter, permease protein AraH. (lbl.gov)
- L-arabinose transport system permease protein AraH. (lbl.gov)
- L-arabinose transport system permease protein araH aka b4460, component of Arabinose porter. (lbl.gov)
- CAS 5328-37-0, L Arabinose, Food Grade L-Arabinose manufacturer supplier in China, offering Ready to Ship Food Additives L-Arabinose Powder CAS 5328-37-0 with Low Price L Arabinose, Non-GMO Textured Pea Protein Vegetarian Meat/Textured Pea Protein 80%, Non-GMO Soy Bean Fiber Soy Bean Dietary Fiber for Meat and Sausages Ketchup and so on. (manodehotel.com)
Pathway3
- Because the system is regulated via the internal arabinose level sensed by AraC, internal arabinose must be rapidly depleted by leakage or export from the cell, or by degradation via a non-canonical pathway. (uni-muenchen.de)
- Verhoeven, Maarten D;Bracher, Jasmine M;Nijland, Jeroen G;Bouwknegt, Jonna;Daran, Jean-Marc G;Driessen, Arnold J M;van Maris, Antonius J A;Pronk, Jack T 2018-05-31 00:00:00 ABSTRACT Cas9-assisted genome editing was used to construct an engineered glucose-phosphorylation-negative S. cerevisiae strain, expressing the Lactobacillus plantaruml-arabinose pathway and the Penicillium chrysogenum transporter PcAraT. (deepdyve.com)
- ABSTRACT Cas9-assisted genome editing was used to construct an engineered glucose-phosphorylation-negative S. cerevisiae strain, expressing the Lactobacillus plantaruml-arabinose pathway and the Penicillium chrysogenum transporter PcAraT. (deepdyve.com)
Xylose and arabinose6
- Thus, a P. putida S12-derived strain was obtained that efficiently utilizes the three main sugars present in lignocellulosic hydrolysate: glucose, xylose, and arabinose. (nih.gov)
- or glucose, xylose, and arabinose (C). Data points are the averages of the results of duplicate measurements. (nih.gov)
- and producing xylitol with high yield based on repeated batch fermentation using a hydrolysate containing xylose and arabinose as a carbon source. (freepatentsonline.com)
- In addition, the present invention relates to an active carbon produced by carbonization and activation of a hydrolysate remainder of a tropical fruit shell, the byproduct of xylose and arabinose production, at a certain temperature and a preparation method of the same. (freepatentsonline.com)
- The present invention relates to a method of producing xylitol using a hydrolysate containing xylose and arabinose prepared from a byproduct of a tropical fruit biomass. (freepatentsonline.com)
- Wild-type strains of Saccharomyces cerevisiae , the organism used in industrial ethanol production, cannot ferment xylose and arabinose. (asm.org)
Synthesis5
- A classic method for the organic synthesis of arabinose from glucose is the Wohl degradation. (wikipedia.org)
- One-pot multienzymatic reactions have been performed for the synthesis of 1-deoxy-D-fructose 6-phosphate, 1,2-dideoxy-D-arabino-hept-3-ulose 7-phosphate, D-fructose 6-phosphate and D-arabinose 5-phosphate. (csic.es)
- L-arabinose is more common than D-arabinose in nature, it can be used as pharmaceutical intermediate, preparing culture medium and be used for synthesis in the flavour industry. (cbaextract.com)
- 4.L-arabinose can be used as raw material for the synthesis of fine chemicals as well as pharmaceutical intermediate. (cbaextract.com)
- Primer extension analysis and transcriptional fusions showed that the synthesis of ara mRNA is induced by L-arabinose and repressed by glucose. (core.ac.uk)
Abstract1
- abstract = "Five enantiopure allylsilanes la-e have been prepared, with arabinose-derived alcohols methyl and benzyl 3,4-O-isopropylidene- and 3,4-O-methylene-β-L-arabinopyranosides as chiral auxiliaries, and subjected to the asymmetric Hosomi - Sakurai reaction. (elsevier.com)
Food Additive3
- 3.Arabinose, as a low calorie sweetener, is approved to be used as a safe food additive by US FDA and Japan. (cbaextract.com)
- Arabinose, Food Additive, Food Sweetener manufacturer / supplier in China, offering China Supply Sweeteners Food Grade D (-) -Arabinose Arabinose, 99% High Purity Veterinary Drugs CAS 1392-21-8 Sineptina, 99% High Purity Powder Additive CAS: 51-35-4 L-Hydroxyproline and so on. (made-in-china.com)
- Raw Material, Health Food, Food Additive manufacturer supplier in China, offering Food Grade L-Arabinose (Plant Derived) with Good Service 5328-37-0, High Quality Rocuronium Bromide Powder CAS. (manodehotel.com)
Sweetener2
- As a low-calorie sweetener [2] [3], L-arabinose has been approved by the American Medical Society and Japanese Ministry of Health to be used as an anti-obesity agent, nutritional supplements or prescription drugs. (scirp.org)
- 4.Arabinose is a non-calorie natural compound sweetener, and it is good to anti-obesity, prevention & cure. (cbaextract.com)
Hydrolysis2
- L-arabinose is mainly produced by chemical or enzymatic hydrolysis of hemicellulose. (scirp.org)
- Mixtures of the α-L-arabinofuranosidases, with specific action on AXOS, have been combined with β-xylosidase and β-xylanase to obtain an optimal mixture for hydrolysis of arabinoxylan to L-arabinose and D-xylose. (megazyme.com)
Mannose2
- Carbo Sep Coregel 87P and molecule of arabinose & mannose? (interchim.com)
- The 87P would only provide ~30 seconds between peaks of Mannose and Arabinose. (interchim.com)
Crystalline3
- L-arabinose is an odorless white needle-like crystalline at room temperature, and it is soluble in water, slightly soluble in alcohol, insoluble in ether, methanol and acetone. (scirp.org)
- High-purity L-Arabinose is a while, crystalline powder. (dealchemical.com)
- L-Arabinose is a white crystalline powder with a sweetness about 50% of that of sugar. (theingredienthouse.com)
AraC1
- To finish a construction with Pbad/araC, which is a promoter whose activity depends on addition of arabinose in medium. (igem.org)
Biomass1
- For cost-effective and efficient ethanol production from lignocellulosic fractions of plant biomass, the conversion of not only major constituents, such as glucose and xylose, but also less predominant sugars, such as l -arabinose, is required. (asm.org)
Substrate1
- Our synthetic endeavours en route to C-glycosidic derivatives mimicking either the transition state of glycosyl transfer or ArnT natural substrate will be presented. (eurocarb2019.com)
Anaerobic growth3
- The resulting S. cerevisiae strain exhibited high rates of arabinose consumption (0.70 g h −1 g [dry weight] −1 ) and ethanol production (0.29 g h −1 g [dry weight] −1 ) and a high ethanol yield (0.43 g g −1 ) during anaerobic growth on l -arabinose as the sole carbon source. (asm.org)
- This strain, which showed a growth rate of 0.26 h−1 on l-arabinose in aerobic batch cultures, was subsequently evolved for anaerobic growth on l-arabinose in the presence of d-glucose and d-xylose. (deepdyve.com)
- Gal2N376T, T89I and Gal2T89I variants showed a lower Km for l-arabinose and a higher Km for d-glucose than wild-type Gal2, while reverting Gal2N376T, T89I to Gal2N376 in an evolved strain negatively affected anaerobic growth on arabinose. (deepdyve.com)
C5H10O51
- L-arabinose (C5H10O5, Mr = 150.13), an aldopentose, is initially isolated from arabic gum via complex chemical and physical methods [5] [6]. (scirp.org)
Mutants1
- Arabinose mutants were tested for the ability to form nitrogen-fixing nodules on alfalfa, and to compete with the wild-type for nodule occupancy. (microbiologyresearch.org)
Metabolic2
- Studies using D-[U-14C]-labelled arabinose showed that it was fermented to pyruvate, formate, lactate and acetate, whereas the principal metabolic products of D-[U-14C]-labelled glucose were acetate and formate. (nih.gov)
- Although metabolic and evolutionary engineering has enabled the efficient alcoholic fermentation of xylose under anaerobic conditions, the conversion of l -arabinose into ethanol by engineered S. cerevisiae strains has previously been demonstrated only under oxygen-limited conditions. (asm.org)
Sugar Blocker1
- L-Arabinose is a natural sugar blocker. (kaz-international.com)
Absorption1
- As a functional additive, L-arabinose can be used in medical and pharmaceutical applications for the treatment of diseases such as diabetes, chronic constipation, mineral absorption disorder and secondary bile acid formation disorder. (scirp.org)
Pectin1
- However, L-arabinose is in fact more common than D-arabinose in nature and is found in nature as a component of biopolymers such as hemicellulose and pectin. (wikipedia.org)
Sugars2
- Although glucose and xylose are often the predominant sugars in these feedstocks, the economically efficient production of ethanol also requires the conversion of smaller carbohydrate fractions, such as l -arabinose, at high rates and yields ( 9 , 23 ). (asm.org)
- As opposed to most other sugars, D-arabinose, featured here, is actually less commonly found in nature than L-arabinose. (goldbio.com)
Fermentation3
- All control strains of E. faecium were easily differentiated from Enterococcus faecalis and Enterococcus durans on the basis of arabinose fermentation on CAA. (asm.org)
- This study reports the first case of fast and efficient anaerobic alcoholic fermentation of l -arabinose by an engineered S. cerevisiae strain. (asm.org)
- The next challenge is the fermentation of other pentoses, such as l -arabinose. (asm.org)
Concentration1
- This page describes the relationship between Arabinose concentration in the medium with light output. (igem.org)
Degradation1
- We find that rapid off-switching does not depend on internal arabinose degradation. (uni-muenchen.de)
Concentrations4
- phoA expression can be modulated over a wide range of inducer (arabinose) concentrations and reduced to extremely low levels by the presence of glucose, which represses expression. (asm.org)
- The highest concentrations of L-Arabinose can be found in instant coffee, wine and sake. (techconnect.org)
- Evolution of luminescence with time at different Arabinose concentrations. (igem.org)
- tn10 with time at different Arabinose concentrations. (igem.org)
Binds1
- The L-arabinose molecule binds in a chair conformation with the OH(1) axial ((beta)-anomer). (rice.edu)
Inducer1
- While there is significant, inducer-dependent cell-to-cell variation in the timing of the on-switching, the off-switching triggered by sudden removal of arabinose is homogeneous and rapid. (uni-muenchen.de)
Cytosine2
- When checking for a misdiagnosis of Cytosine arabinose syndrome or confirming a diagnosis of Cytosine arabinose syndrome, it is useful to consider what other medical conditions might be possible misdiagnoses or other alternative conditions relevant to diagnosis. (rightdiagnosis.com)
- These alternate diagnoses of Cytosine arabinose syndrome may already have been considered by your doctor or may need to be considered as possible alternative diagnoses or candidates for misdiagnosis of Cytosine arabinose syndrome. (rightdiagnosis.com)
Displaystyle1
- L-arabinose ⇌ {\displaystyle \rightleftharpoons } \ rightleftharpoons L-ribulose. (sacredheartrs.org)
Converts2
- This work reports the first one-pot enzymatic cascade which completely converts l -arabinose to l-ribulose using four reactions catalyzed by. (sacredheartrs.org)
- PvUAM1 encodes a switchgrass homolog of UDP-arabinose mutase, which converts UDP-Ara p to UDP-Ara f . (frontiersin.org)
Hemicellulose1
- As a component of heteropolysaccharides that is found in gum, hemicellulose, bacterial polysaccharides and some glycoside, L-arabinose exists in the form of β-L-arabinose. (scirp.org)
Isomerization1
- Catalyzes the reversible aldol-ketol isomerization between D-ribulose 5-phosphate (Ru5P) and D-arabinose 5-phosphate (A5P). (mybiosource.com)
Polysaccharide1
- A polysaccharide that is mostly a polymer of arabinose. (eurelectric.org)
Trehalose1
- 83% L-Arabinose Plus, 17% Trehalose plus spray micro cellulose film coating (veg. (sweet-cures.com)
Promoter2
- The araE low affinity L-arabinose transport promoter. (uniprot.org)
- It is under the control of an Arabinose induced promoter. (igem.org)
Yeast1
- Some organic acid tests check for the presence of arabinose, which may indicate overgrowth of intestinal yeast such as Candida albicans or other yeast/fungus species. (wikipedia.org)