Arabidopsis Proteins
Arabidopsis
Gene Expression Regulation, Plant
Plants, Genetically Modified
Plant Proteins
Molecular Sequence Data
Sequence Homology, Amino Acid
RNA, Plant
Amino Acid Sequence
Plant Leaves
Mutation
Sequence Alignment
Plant Roots
Two-Hybrid System Techniques
Base Sequence
Cloning, Molecular
Seeds
Amino Acid Motifs
Databases, Protein
Indoleacetic Acids
Plant Growth Regulators
Germination
Carrier Proteins
Meristem
Protein Binding
Abscisic Acid
Plant Epidermis
Ethylenes
Phenotype
Oxylipins
Salicylic Acid
Cotyledon
Genetic Complementation Test
MADS Domain Proteins
Mutagenesis, Insertional
Cytokinins
Chloroplasts
AGAMOUS Protein, Arabidopsis
Pseudomonas syringae
Plant Stomata
Plastids
Plant Stems
Gibberellins
Oryza sativa
Glucosinolates
Multigene Family
Transcription Factors
Brassicaceae
Chromosomes, Plant
Tobacco
Phytochrome B
Plant Immunity
Gene Expression Profiling
Gravitropism
Signal Transduction
Photoperiod
DNA, Complementary
Plant Vascular Bundle
Brassinosteroids
Ecotype
Chlorophyll
Stress, Physiological
Phytochrome A
Protoplasts
Anthocyanins
Droughts
Disease Resistance
Inflorescence
Oomycetes
Brassica
Salt-Tolerance
Phototropism
Chromosome Mapping
Photosynthesis
Steroids, Heterocyclic
Pollen Tube
Gene Expression Regulation, Developmental
Recombinant Fusion Proteins
Promoter Regions, Genetic
Botrytis
Thylakoids
DNA Primers
Plants
Cryptochromes
Mesophyll Cells
RNA, Messenger
Protein Transport
Chloroplast Proteins
Green Fluorescent Proteins
Conserved Sequence
Sequence Analysis, DNA
Phloem
Plant Infertility
Models, Biological
Gene Knockout Techniques
Cell Wall
Gene Expression Regulation, Enzymologic
Alleles
Evolution, Molecular
Oligonucleotide Array Sequence Analysis
Zeatin
Protein Structure, Tertiary
Lycopersicon esculentum
Adaptation, Physiological
Plant Physiological Phenomena
Reverse Transcriptase Polymerase Chain Reaction
Starch
Waxes
Cholestanols
Separation of shoot and floral identity in Arabidopsis. (1/11716)
The overall morphology of an Arabidopsis plant depends on the behaviour of its meristems. Meristems derived from the shoot apex can develop into either shoots or flowers. The distinction between these alternative fates requires separation between the function of floral meristem identity genes and the function of an antagonistic group of genes, which includes TERMINAL FLOWER 1. We show that the activities of these genes are restricted to separate domains of the shoot apex by different mechanisms. Meristem identity genes, such as LEAFY, APETALA 1 and CAULIFLOWER, prevent TERMINAL FLOWER 1 transcription in floral meristems on the apex periphery. TERMINAL FLOWER 1, in turn, can inhibit the activity of meristem identity genes at the centre of the shoot apex in two ways; first by delaying their upregulation, and second, by preventing the meristem from responding to LEAFY or APETALA 1. We suggest that the wild-type pattern of TERMINAL FLOWER 1 and floral meristem identity gene expression depends on the relative timing of their upregulation. (+info)An Arabidopsis 14-3-3 protein can act as a transcriptional activator in yeast. (2/11716)
The 14-3-3 proteins are a group of highly conserved and widely distributed eukaryotic proteins with diverse functions. One 14-3-3 protein, AFT1 from Arabidopsis thaliana, was found to be able to activate transcription in yeast. When fused to the DNA-binding domain of a bacterial protein LexA, AFT1 can activate transcription of reporter genes that contain LexA operator sequences in their promoters. Although the in vivo function of AFT1 is not completely known, its similarity to previously identified proteins found in transcription complexes of Arabidopsis and maize suggests that AFT1 and some other 14-3-3 proteins may activate gene expression in other systems as well. (+info)RAD53 regulates DBF4 independently of checkpoint function in Saccharomyces cerevisiae. (3/11716)
The Cdc7p and Dbf4p proteins form an active kinase complex in Saccharomyces cerevisiae that is essential for the initiation of DNA replication. A genetic screen for mutations that are lethal in combination with cdc7-1 led to the isolation of seven lsd (lethal with seven defect) complementation groups. The lsd7 complementation group contained two temperature-sensitive dbf4 alleles. The lsd1 complementation group contained a new allele of RAD53, which was designated rad53-31. RAD53 encodes an essential protein kinase that is required for the activation of DNA damage and DNA replication checkpoint pathways, and that is implicated as a positive regulator of S phase. Unlike other RAD53 alleles, we demonstrate that the rad53-31 allele retains an intact checkpoint function. Thus, the checkpoint function and the DNA replication function of RAD53 can be functionally separated. The activation of DNA replication through RAD53 most likely occurs through DBF4. Two-hybrid analysis indicates that the Rad53p protein binds to Dbf4p. Furthermore, the steady-state level of DBF4 message and Dbf4p protein is reduced in several rad53 mutant strains, indicating that RAD53 positively regulates DBF4. These results suggest that two different functions of the cell cycle, initiation of DNA replication and the checkpoint function, can be coordinately regulated through the common intermediate RAD53. (+info)Overexpression of a novel Arabidopsis gene related to putative zinc-transporter genes from animals can lead to enhanced zinc resistance and accumulation. (4/11716)
We describe the isolation of an Arabidopsis gene that is closely related to the animal ZnT genes (Zn transporter). The protein encoded by the ZAT (Zn transporter of Arabidopsis thaliana) gene has 398 amino acid residues and is predicted to have six membrane-spanning domains. To obtain evidence for the postulated function of the Arabidopsis gene, transgenic plants with the ZAT coding sequence under control of the cauliflower mosaic virus 35S promoter were analyzed. Plants obtained with ZAT in the sense orientation exhibited enhanced Zn resistance and strongly increased Zn content in the roots under high Zn exposure. Antisense mRNA-producing plants were viable, with a wild-type level of Zn resistance and content, like plants expressing a truncated coding sequence lacking the C-terminal cytoplasmic domain of the protein. The availability of ZAT can lead to a better understanding of the mechanism of Zn homeostasis and resistance in plants. (+info)Discrete domains mediate the light-responsive nuclear and cytoplasmic localization of Arabidopsis COP1. (5/11716)
The Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) protein plays a critical role in the repression of photomorphogenesis during Arabidopsis seedling development. We investigated the control of COP1 partitioning between nucleus and cytoplasm, which has been implicated in the regulation of COP1 activity, by using fusion proteins between COP1 and beta-glucuronidase or the green fluorescent protein. Transient expression assays using onion epidermal cells and data from hypocotyl cells of stably transformed Arabidopsis demonstrated that COP1 carries a single, bipartite nuclear localization signal that functions independently of light. Nuclear exclusion was mediated by a novel and distinct signal, bordering the zinc-finger and coiled-coil motifs, that was able to redirect a heterologous nuclear protein to the cytoplasm. The cytoplasmic localization signal functioned in a light-independent manner. Light regulation of nuclear localization was reconstituted by combining the individual domains containing the nuclear localization signal and the cytoplasmic localization signal; the WD-40 repeat domain of COP1 was not required. However, phenotypic analysis of transgenic seedlings suggested that the constitutively nuclear-localized WD-40 repeat domain was able to mimic aspects of COP1 function, as indicated by exaggerated hypocotyl elongation under light conditions. (+info)IAR3 encodes an auxin conjugate hydrolase from Arabidopsis. (6/11716)
Amide-linked conjugates of indole-3-acetic acid (IAA) are putative storage or inactivation forms of the growth hormone auxin. Here, we describe the Arabidopsis iar3 mutant that displays reduced sensitivity to IAA-Ala. IAR3 is a member of a family of Arabidopsis genes related to the previously isolated ILR1 gene, which encodes an IAA-amino acid hydrolase selective for IAA-Leu and IAA-Phe. IAR3 and the very similar ILL5 gene are closely linked on chromosome 1 and comprise a subfamily of the six Arabidopsis IAA-conjugate hydrolases. The purified IAR3 enzyme hydrolyzes IAA-Ala in vitro. iar 3 ilr1 double mutants are more resistant than either single mutant to IAA-amino acid conjugates, and plants overexpressing IAR3 or ILR1 are more sensitive than is the wild type to certain IAA-amino acid conjugates, reflecting the overlapping substrate specificities of the corresponding enzymes. The IAR3 gene is expressed most strongly in roots, stems, and flowers, suggesting roles for IAA-conjugate hydrolysis in those tissues. (+info)The CLAVATA1 receptor-like kinase requires CLAVATA3 for its assembly into a signaling complex that includes KAPP and a Rho-related protein. (7/11716)
The CLAVATA1 (CLV1) and CLAVATA3 (CLV3) genes are required to maintain the balance between cell proliferation and organ formation at the Arabidopsis shoot and flower meristems. CLV1 encodes a receptor-like protein kinase. We have found that CLV1 is present in two protein complexes in vivo. One is approximately 185 kD, and the other is approximately 450 kD. In each complex, CLV1 is part of a disulfide-linked multimer of approximately 185 kD. The 450-kD complex contains the protein phosphatase KAPP, which is a negative regulator of CLV1 signaling, and a Rho GTPase-related protein. In clv1 and clv3 mutants, CLV1 is found primarily in the 185-kD complex. We propose that CLV1 is present as an inactive disulfide-linked heterodimer and that CLV3 functions to promote the assembly of the active 450-kD complex, which then relays signal transduction through a Rho GTPase. (+info)Mutations in FIE, a WD polycomb group gene, allow endosperm development without fertilization. (8/11716)
A fundamental problem in biology is to understand how fertilization initiates reproductive development. Higher plant reproduction is unique because two fertilization events are required for sexual reproduction. First, a sperm must fuse with the egg to form an embryo. A second sperm must then fuse with the adjacent central cell nucleus that replicates to form an endosperm, which is the support tissue required for embryo and/or seedling development. Here, we report cloning of the Arabidopsis FERTILIZATION-INDEPENDENT ENDOSPERM (FIE) gene. The FIE protein is a homolog of the WD motif-containing Polycomb proteins from Drosophila and mammals. These proteins function as repressors of homeotic genes. A female gametophyte with a loss-of-function allele of fie undergoes replication of the central cell nucleus and initiates endosperm development without fertilization. These results suggest that the FIE Polycomb protein functions to suppress a critical aspect of early plant reproduction, namely, endosperm development, until fertilization occurs. (+info)1. Innate immunity: This is the body's first line of defense against infection, and it involves the recognition and elimination of pathogens by cells and proteins that are present from birth.
2. Acquired immunity: This type of immunity develops over time as a result of exposure to pathogens, and it involves the production of antibodies and other immune cells that can recognize and eliminate specific pathogens.
3. Cell-mediated immunity: This is a type of immunity that involves the activation of immune cells, such as T cells and macrophages, to fight off infection.
4. Genetic resistance: Some individuals may have a genetic predisposition to disease resistance, which can be influenced by their ancestry or genetic makeup.
5. Environmental factors: Exposure to certain environmental factors, such as sunlight, clean water, and good nutrition, can also contribute to disease resistance.
Disease resistance is an important concept in the medical field, as it helps to protect against infectious diseases and can reduce the risk of illness and death. Understanding how disease resistance works can help healthcare professionals develop effective strategies for preventing and treating infections, and it can also inform public health policies and interventions aimed at reducing the burden of infectious diseases on individuals and communities.
Seagrass
BURP domain
Fasciclin domain
Methyl-CpG-binding domain
Arabinogalactan protein
Villin-1
PIKFYVE
Elizabeth Haswell
Chalcone synthase
TRNA nucleotidyltransferase
Phragmoplast
GrpE
Seipin
Arabidopsis thaliana
Dehydrin
Polycomb-group proteins
Joseph Jez
Eukaryotic ribosome
Cytokinin signaling and response regulator protein
Cyclic nucleotide
Wojciech Karlowski
Exitron
SACM1L
UVR8
Fig4
Philip A. Rea
ETHE1
Dicer
ABC model of flower development
PAS domain
Auxin binding protein
Pal Maliga
Myriam Charpentier
Ornithine cyclodeaminase
Ubiquitin-like protein
Xanthoferrin
Palmitoyl-CoA hydrolase
Chlorophyll fluorescence
PRC2
Nitrilase
Gene delivery
CDS1 (gene)
Botrytis-induced kinase 1
N-end rule
Pattern recognition receptor
WNK1
Glabrousness
SOBER1
GeneNetwork
Manoj Majee
BZIP intron RNA motif
CAAT box
Alpha-aminoadipic semialdehyde synthase
Glutathione synthetase
Green Revolution
Microautophagy
Zinc in biology
Tiling array
Epigenomics
Arabidopsis thaliana MYB-28 protein (ABIN1657645)
Understanding Arabidopsis ion homeostasis in the post-genomic era : assigning function to two proteins involved in iron...
A new link between stress response and nucleolar function during pollen development in Arabidopsis mediated by AtREN1 protein |...
GelMap: The protein complex proteome of Arabidopsis mitochondria
Recombinant Arabidopsis thaliana Cadmium-induced protein AS8 (At4g19070), Baculovirus | Labstore
Overexpression of X Intrinsic Protein 1;1 in |i|Nicotiana tabacum|/i| and Arabidopsis reduces boron allocation to shoot sink...
SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production | Nature
Rapid isolation of Arabidopsis chloroplasts and their use for in vitro protein import assays. - Oxford Neuroscience
Biology
Functional conservation of rice OsNF-YB/YC and Arabidopsis AtNF-YB/YC proteins in the regulation of flowering time<...
Dimerization of the ETO1 family proteins plays a crucial role in regulating ethylene biosynthesis in Arabidopsis thaliana<...
Arabidopsis accelerated cell death 11, ACD11, is a ceramide-1-phosphate transfer protein and intermediary regulator of...
Plants | Free Full-Text | Shedding the Last Layer: Mechanisms of Root Cap Cell Release
Jeremy Scutenaire, Ph.D. | About NIAMS | NIAMS
SGN Unigene - Show All Stored BLAST Hits - Sol Genomics Network
Comparative Analysis of SWIRM Domain-Containing Proteins in Plants
Publication Detail
Biosynthesis and Regulation of Antioxidant Flavonolignans in Milk Thistle | IntechOpen
Larry Fliegel | Home
IOBC-WPRS Bulletin Vol. 89, 2013 - IOBC-WPRS
Amy Hark | Muhlenberg College
UBIRA ETheses - Items where Year is 2014
Jaideep Mathur - Google Scholar
AT3G21390 gene cDNA ORF clone, Arabidopsis thaliana(thale cress) - GenScript
Thesis by Lamberti, Giorgia
Alan C. Christensen - Google Scholar
YRC Public Data Repository - Protein Structure Prediction - Protein Overview
SMART: Schnipsel domain HAT
Thaliana12
- Its mutational inactivation in Arabidopsis thaliana results in a photosystem II (PHII)-less phenotype. (nih.gov)
- A nuclear-encoded protein of prokaryotic origin is essential for the stability of photosystem II in Arabidopsis thaliana. (nih.gov)
- Two projects studying different aspects of iron deficiency in the model plant Arabidopsis thaliana are detailed here. (umsystem.edu)
- Arabidopsis (Arabidopsis thaliana) GRIK1 and GRIK2, which were first characterized as geminivirus Rep interacting kinases, are phylogenetically related to SNF1 and AMPK activating kinases. (ncsu.edu)
- In Arabidopsis thaliana, several PRC2 complexes with different compositions were identified, each controlling a particular developmental program. (tau.ac.il)
- Furthermore, the secondary structures of pri-miRNAs differed between wild-type Arabidopsis thaliana and chr2 mutants. (nature.com)
- In Arabidopsis thaliana, two NF-YB (AtNF-YB2 and AtNF-YB3) and five NF-YC (AtNF-YC1, AtNF-YC2, AtNF-YC3, AtNF-YC4, and AtNF-YC9) genes regulate photoperiodic flowering by interacting with other AtNF-Y subunit proteins. (korea.ac.kr)
- By utilising a state-of-the-art liquid chromatography/tandem mass spectrometry (LC-MS/MS) approach, rich pollen coat proteomic profiles were obtained for Arabidopsis thaliana, Arabidopsis lyrata, and Brassica oleracea, which greatly extended previous datasets. (aber.ac.uk)
- Arabidopsis (Arabidopsis thaliana) contains three SRP homologs (SRP1, SRP2, and SRP3) that share sequence identities with small rubber particle proteins of the rubber tree (Hevea brasiliensis). (elsevierpure.com)
- ETHYLENE OVERPRODUCER1 (ETO1), ETO1-LIKE1 (EOL1), and EOL2 are members of the Broad complex, Tramtrack, Bric-a-brac (BTB) protein family that collectively regulate type-2 1-aminocyclopropane-1-carboxylic acid synthase (ACS) activity in Arabidopsis thaliana. (ncu.edu.tw)
- The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments. (nih.gov)
- Las especies de Arabidopsis más estudiadas, la Arabidopsis thaliana, se utilizan generalmente en laboratorios de experimentación. (bvsalud.org)
Recombinant3
- Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antibodies-online.com)
- In this study, we used recombinant proteins produced in bacteria to show that both GRIKs specifically bind to the SnRK1 catalytic subunit and phosphorylate the equivalent threonine residue in its activation loop in vitro. (ncsu.edu)
- The demonstration that recombinant Arabidopsis variant P protein has NO-synthesizing activity was a critical piece of evidence leading to the above conclusion. (nih.gov)
Transgenic4
- Analysing endogenous FIE and transgenic gFIE-green fluorescent protein fusion protein (gFIE-GFP) showed that FIE accumulates in the nuclei of every cell type examined. (tau.ac.il)
- To investigate the functional conservation of these NF-YB and NF-YC genes in rice and Arabidopsis, we analyzed the flowering phenotypes of transgenic plants overexpressing the respective OsNF-YB and OsNF-YC genes in Arabidopsis mutants. (korea.ac.kr)
- SRP-overexpressing transgenic Arabidopsis plants (35S:SRP1, 35S:SRP2, and 35S:SRP3) exhibited higher vegetative and reproductive growth and markedly better tolerance to drought stress than wild-type Arabidopsis. (elsevierpure.com)
- However, domain-swapping analysis in transgenic Arabidopsis suggests that the BTB domain of ETO1 is essential but not sufficient for a full spectrum of ETO1 function. (ncu.edu.tw)
Overexpression6
- A nonparametric mean-variance smoothing method to assess Arabidopsis cold stress transcriptional regulator CBF2 overexpression microarray data. (nih.gov)
- Overexpression of the CBF2 transcriptional activator in Arabidopsis suppresses the responsiveness of leaf tissue to the stress hormone ethylene. (nih.gov)
- Overexpression of X Intrinsic Protein 1;1 in Nicotiana tabacum and Arabidopsis reduces boron allocation to shoot sink tissues. (bvsalud.org)
- Here, we demonstrated that overexpression of NtXIP1;1 in Nicotiana tabacum by the En2pPMA4 or the 35S CaMV promoter and in Arabidopsis , which does not contain any XIP gene , by the 35S CaMV promoter, resulted in boron (B)- deficiency symptoms such as death of the shoot apical meristem , infertile flowers , and puckered leaves. (bvsalud.org)
- Overexpression of OsNF-YB8/10/11 and OsNF-YC2 complemented the late flowering phenotype of Arabidopsis nf-yb2 nf-yb3 and nf-yc3 nf-yc4 nf-yc9 mutants, respectively. (korea.ac.kr)
- Overexpression of ETO1 F466I in Arabidopsis results in a constitutive triple response phenotype in dark-grown seedlings. (ncu.edu.tw)
Mutants3
- The first project describes the isolation and characterization of the Arabidopsis frd4-1 and frd4-2 mutants that do not induce Fe(III) chelate reductase activity in their roots in response to iron deficiency. (umsystem.edu)
- Using the Rep protein of Tomato golden mosaic virus (TGMV AL1), we show that GRS mutants are not infectious in plants and do not support viral genome replication in tobacco protoplasts. (ncsu.edu)
- GRS mutants are competent for protein-protein interactions and for both double- and single-stranded DNA binding, indicating that the mutations did not impair its global conformation. (ncsu.edu)
Homologs1
- Ada2-types SWIRM domain-containing proteins are homologs of transcriptional adaptor ADA2a, which promotes histone lysine acetylation and transcriptional activation and acts as a molecular scaffold within the SAGA remodeling complex [ 16 ]. (hindawi.com)
Phenotype1
- Peptide Aptamers That Bind to Geminivirus Replication Proteins Confer a Resistance Phenotype to Tomato Yellow Leaf Curl Virus and Tomato Mottle Virus Infection in Tomato. (ncsu.edu)
Genes4
- Arabidopsis has been used as a source for virus-resistant genes that derive from alterations in essential host factors. (ncsu.edu)
- Our data indicate that some OsNF-YB and OsNF-YC genes are functional equivalents of AtNF-YB2/3 and AtNF-YC3/4/9 genes, respectively, and suggest functional conservation of Arabidopsis and rice NF-Y genes in the control of flowering time. (korea.ac.kr)
- The genes encoding SWIRM domain proteins in Oryza sativa are widely expressed, especially in pistils. (hindawi.com)
- Long non-coding RNAs (lncRNAs), a kind of non-coding RNA (ncRNA) with a transcript of more than 200 nucleotides in length in eukaryotic cells, have been found to regulate the expression of protein-coding genes that are involved in some critical signaling events, such as inflammatory, pathological, and immunological responses. (bvsalud.org)
Yeast6
- The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antibodies-online.com)
- The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antibodies-online.com)
- A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antibodies-online.com)
- Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antibodies-online.com)
- Here, we demonstrate that as opposed to yeast and metazoans the plant exosome core possesses an unanticipated functional plasticity and present a genome-wide atlas of Arabidopsis exosome targets. (nih.gov)
- In Schizosaccharomyces pombe the Na + /H+ exchanger (sod2) moves Na + out of the cell in exchange for H + . This protein functions in osmotolerance in yeast and plants. (ualberta.ca)
Regulator1
- Arabidopsis accelerated cell death 11, ACD11, is a ceramide-1-phosphate transfer protein and intermediary regulator of phytoceramide levels. (anl.gov)
Accession1
- A VIGS screen identifies immunity in the Arabidopsis Pla-1 accession to viruses in two different genera of the Geminiviridae. (ncsu.edu)
Amino Acids1
- We have examined amino acids important in cation binding and transport in this protein. (ualberta.ca)
Biosynthesis2
- Although ETO1 and EOL1/EOL2 encode structurally related proteins, genetic studies suggest that they do not play an equivalent role in regulating ethylene biosynthesis. (ncu.edu.tw)
- Our findings reveal the mechanistic role of protein-protein interactions of ETO1 and EOL1/EOL2 that is crucial for their biological function in ethylene biosynthesis. (ncu.edu.tw)
20022
- This report was developed solely by the participants of the Protein Production Workshop, held March 7-8, 2002. (nih.gov)
- The NIGMS convened the Protein Production Workshop in March 2002 to specifically address high-throughput methods for cloning, production, and purification of proteins suitable for X-ray crystallography and NMR studies. (nih.gov)
Target proteins3
- Although other issues (e.g., the selection and crystallization of target proteins, the collection and analysis of structural data, and the integration of databases) are critical to the overall research effort, NIGMS chose not to focus on these topics at this meeting. (nih.gov)
- The target proteins for PSI include proteins whose functions are known or unknown. (nih.gov)
- He emphasized that genome-directed selection of target proteins, registration of target proteins, and timely publication of coordinates and release of data are important. (nih.gov)
Vitro5
- Rapid isolation of Arabidopsis chloroplasts and their use for in vitro protein import assays. (ox.ac.uk)
- In vitro chloroplast protein import assays have been performed since the late 1970s, initially with plant species (e.g., pea and spinach) that readily provide an abundant source of starting material and also, subsequently, a good yield of chloroplasts for import assays. (ox.ac.uk)
- The protocol is also optimized for work with material from young plants (10-14 days old), when protein import is believed to be at its peak, and so plant growth can be conducted in vitro on Murashige and Skoog medium. (ox.ac.uk)
- In vitro and in planta protein-protein analyses revealed that OsNF-YB8/10/11 and OsNF-YC1/2/4/6/7 interact with AtNF-YC3/4/9 and AtNF-YB2/3, respectively. (korea.ac.kr)
- Arabidopsis SRPs appeared to be LD-associated proteins and displayed polymerization properties in vivo and in vitro. (elsevierpure.com)
Chromatin4
- Polycomb group (PcG) proteins are evolutionarily conserved chromatin modifiers that regulate developmental pathways in plants. (tau.ac.il)
- SWIRM domain-containing proteins make up large multisubunit complexes by interacting with other chromatin modification factors and may have an important function in plants. (hindawi.com)
- Chromodomains were commonly found in proteins associated with the remodeling and manipulation of chromatin, mediating specific interactions with proteins and RNA by recognizing lysine methylation in histone tails [ 8 , 9 ]. (hindawi.com)
- Swi3p-type SWIRM domain-containing proteins are homologous to the ATP-dependent chromatin remodeling complexes SWI/SNF. (hindawi.com)
Complex1
- This paper (Cell 113, 115-125, April 4, 2003) reports results of experiments that together strongly support the conclusion that, in metazoan cells, formation of a complex consisting of the GTP binding protein Ran, the exportin Crm1, and the DNA helicase MCM plays a critical role in limiting DNA replication to a single round each cell cycle. (nih.gov)
Accessions4
- We used a virus-induced gene silencing (VIGS) vector derived from the geminivirus Cabbage leaf curl virus (CaLCuV) to assess natural variation in virus-host interactions in 190 Arabidopsis accessions. (ncsu.edu)
- Silencing of CH-42, encoding a protein needed to make chlorophyll, was used as a visible marker to discriminate asymptomatic accessions from those showing resistance. (ncsu.edu)
- Persistent Virus-Induced Gene Silencing in Asymptomatic Accessions of Arabidopsis. (ncsu.edu)
- 2006. Multivariate analysis of protein profiles of metal hyperaccumulator Thlaspi caerulescens accessions . (hutton.ac.uk)
Mammalian5
- A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antibodies-online.com)
- In mammalian tissues the main protein responsible for this is the Na + /H + exchanger. (ualberta.ca)
- The Na + /H + exchanger is a mammalian plasma membrane protein that exchanges one intracellular proton for an extracellular sodium. (ualberta.ca)
- We have a special interest in the Na + /H + exchanger in the mammalian heart, because the protein is important in heart disease. (ualberta.ca)
- This gene is known to suppress G-protein and mitogen-activated signal transduction in mammalian cells. (nih.gov)
Mutation1
- In C. elegans oocytes, a katanin loss of function mutation proteins and the recent significant advances in deciphering the results in failure to form a bipolar meiotic spindle [ 9 ]. (nih.gov)
Species3
- Amongst species of the Brassicaceae, a growing body of data has revealed that the pollen coat carries a range of proteins, with a number of small cysteine-rich proteins (CRPs) being identified as important regulators of the pollen-stigma interaction. (aber.ac.uk)
- In this study, 67 SWIRM domain-containing proteins from 6 plant species were identified and analyzed. (hindawi.com)
- Proteins that originate from plants species belonging to the genus ARABIDOPSIS . (nih.gov)
Nucleolar1
- 2005. Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions . (hutton.ac.uk)
Biology1
- Researchers participating in the nascent PSI already are making important advances in the technology of protein production for structural biology. (nih.gov)
Encode2
Proteomic1
- The isolation method has been used not only for protein import assays, but also for proteomic analysis and further subfractionation studies. (ox.ac.uk)
Plants3
- X Intrinsic Proteins (XIP) form the least characterized MIP subfamily in vascular plants . (bvsalud.org)
- The accelerated cell death 11 (acd11) mutant of Arabidopsis provides a genetic model for studying immune response activation and localized cellular suicide that halt pathogen spread during infection in plants. (anl.gov)
- However, little is known about SWIRM domain-containing proteins in plants. (hindawi.com)
Characterization1
- The second project involves the further characterization of the protein FRD3, which was previously shown to be important for the efficient translocation of iron from roots to the shoots. (umsystem.edu)
Developmental1
- Recent studies indicate that plant SWIRM domain-containing proteins function in various plant physiological and developmental processes. (hindawi.com)
Pollen1
- The profiling also uncovered a wide range of other protein families, many of which were enriched in the pollen coat proteomes and had functions associated with signal transduction, cell walls, lipid metabolism and defence. (aber.ac.uk)
Tissues3
- Given that the GRIK proteins only accumulate in young tissues and geminivirus-infected mature leaves, the GRIK-SnRK1 cascade may function in a developmentally regulated fashion and coordinate the unique metabolic requirements of rapidly growing cells and geminivirus-infected cells that have been induced to reenter the cell cycle. (ncsu.edu)
- and Arabidopsis reduces boron allocation to shoot sink tissues. (bvsalud.org)
- Protein quantification analysis revealed that NtXIP1;1 was predominantly expressed in young B-demanding tissues and induced under B-deficient conditions. (bvsalud.org)
Nuclear1
- The production of protein samples that are suitable for X-ray diffraction and/or analysis in solution using nuclear magnetic resonance (NMR) is a major challenge for conducting high-throughput analysis of protein structures. (nih.gov)
Tolerance2
- CBF-dependent and CBF-independent regulatory pathways contribute to the differences in freezing tolerance and cold-regulated gene expression of two Arabidopsis ecotypes locally adapted to sites in Sweden and Italy. (nih.gov)
- Our results suggest that Arabidopsis SRPs play dual roles as positive factors in postgermination growth and the drought stress tolerance response. (elsevierpure.com)
Homeostasis1
- 12. The 78-kD Glucose-Regulated Protein Regulates Endoplasmic Reticulum Homeostasis and Distal Epithelial Cell Survival during Lung Development. (nih.gov)
Regulation4
- In this study, we reveal that ETO1 collaborates with EOL1/2 to play a key role in the regulation of type-2 ACS activity via protein-protein interactions. (ncu.edu.tw)
- In the heart we have examined regulation of expression and activity of the protein. (ualberta.ca)
- Both p90rsk and MAP kinase are key protein kinases involved in regulation of activity of the protein. (ualberta.ca)
- The laboratory continues to be involved in new and exciting studies on regulation of activity and expression of Na + /H + exchange proteins. (ualberta.ca)
Structures5
- The aim of this effort is to determine one or more representative structures from each of the several thousand protein families in nature. (nih.gov)
- The ultimate goals are to understand the evolutionary implications of protein structures and to elucidate their biochemical and biophysical functions in relation to their three-dimensional structures. (nih.gov)
- Knowledge of the protein structures, and development of the corresponding reagents and technologies through the International Structural Proteomics Initiative, will provide the foundation for scientific and technological infrastructure that will broadly and importantly influence biomedical and biomolecular research well into the 21st century. (nih.gov)
- Some experts have suggested that production of these protein samples is the only real barrier to the determination of protein structures using high-throughput methods. (nih.gov)
- The initiative is a cooperative, large-scale effort to determine the structures of unique, non-redundant proteins by using high-throughput methods that will ultimately result in an inventory, and complete coverage, of naturally occurring proteins. (nih.gov)
Kinase2
Interaction1
- Interaction between Geminivirus Replication Protein and the SUMO-Conjugating Enzyme Is Required for Viral Infection. (ncsu.edu)
Important roles1
- LD-associated proteins play important roles in seed development and germination, but their functions in postgermination growth are not well understood. (elsevierpure.com)
Function1
- in the N termini of geminivirus Rep proteins are essential for function. (ncsu.edu)
Histone1
- SANT domains tether to both DNA and proteins and are essential for histone acetyltransferase activity [ 10 , 11 ]. (hindawi.com)
Commonly1
- Generally, the SWIRM domain forms a helix-turn-helix motif commonly found in DNA-binding proteins. (hindawi.com)
Chloroplast1
- Map-based cloning revealed that the frd4 mutations reside in cpFtsY, which encodes a component of one of the pathways responsible for the insertion of proteins into the thylakoid membranes of the chloroplast. (umsystem.edu)
Isolation1
- A prerequisite for this change was an efficient and reliable protocol for the isolation of chloroplasts for use in protein import assays, enabling biochemical approaches to be combined with the genetic potential of the plant. (ox.ac.uk)
Plant4
- Ectopic AL1 expression did not result in broad changes in the sumoylation pattern of plant cells, but specific changes were detected, indicating that AL1 modifies the sumoylation state of selected host proteins. (ncsu.edu)
- During his Ph.D., he studied the impact of one of these YTH-protein in plant development and in response to stress, initiating pioneer works in the role of m6A-binding proteins in the plant kingdom. (nih.gov)
- Plant SWIRM domain proteins can be divided into three distinct types: Swi-type, LSD1-type, and Ada2-type. (hindawi.com)
- These observations indicate that SWIRM domain proteins may play an essential role in plant development and plant responses to environmental stress. (hindawi.com)
Analysis1
- However, the sequencing of the Arabidopsis genome paved the way for an additional model system that is more amenable to genetic analysis, as a complement to the more biochemically orientated models such as pea and spinach. (ox.ac.uk)
Results1
- The results of these experiments showed that all 16 peptide aptamers interact with all or most of the Rep proteins from nine viruses representing the three major Geminiviridae genera and identified two peptide aptamers (A22 and A64) that interact strongly with different regions in the Rep N terminus. (ncsu.edu)
Tomato1
- 2006. Translocation of the Tomato bushy stunt virus P19 protein into the nucleus by ALY proteins compromises its silencing suppressor activity . (hutton.ac.uk)
Membrane2
- In collaboration with others we have elucidated the structure of parts of the membrane domain of the protein. (ualberta.ca)
- Overall, our long-term goal is to understand how ions and coordinated and transported in membrane proteins. (ualberta.ca)