Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.
PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
The functional hereditary units of PLANTS.
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Ribonucleic acid in plants having regulatory and catalytic roles as well as involvement in protein synthesis.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)
Deoxyribonucleic acid that makes up the genetic material of plants.
The genetic complement of a plant (PLANTS) as represented in its DNA.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
The relationships of groups of organisms as reflected by their genetic makeup.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Very young plant after GERMINATION of SEEDS.
The reproductive organs of plants.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
Acetic acid derivatives of the heterocyclic compound indole. (Merck Index, 11th ed)
Any of the hormones produced naturally in plants and active in controlling growth and other functions. There are three primary classes: auxins, cytokinins, and gibberellins.
The initial stages of the growth of SEEDS into a SEEDLINGS. The embryonic shoot (plumule) and embryonic PLANT ROOTS (radicle) emerge and grow upwards and downwards respectively. Food reserves for germination come from endosperm tissue within the seed and/or from the seed leaves (COTYLEDON). (Concise Dictionary of Biology, 1990)
New immature growth of a plant including stem, leaves, tips of branches, and SEEDLINGS.
Transport proteins that carry specific substances in the blood or across cell membranes.
A group of plant cells that are capable of dividing infinitely and whose main function is the production of new growth at the growing tip of a root or stem. (From Concise Dictionary of Biology, 1990)
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Abscission-accelerating plant growth substance isolated from young cotton fruit, leaves of sycamore, birch, and other plants, and from potatoes, lemons, avocados, and other fruits.
A thin layer of cells forming the outer integument of seed plants and ferns. (Random House Unabridged Dictionary, 2d ed)
Derivatives of ethylene, a simple organic gas of biological origin with many industrial and biological use.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Proteins prepared by recombinant DNA technology.
Eighteen-carbon cyclopentyl polyunsaturated fatty acids derived from ALPHA-LINOLENIC ACID via an oxidative pathway analogous to the EICOSANOIDS in animals. Biosynthesis is inhibited by SALICYLATES. A key member, jasmonic acid of PLANTS, plays a similar role to ARACHIDONIC ACID in animals.
A compound obtained from the bark of the white willow and wintergreen leaves. It has bacteriostatic, fungicidal, and keratolytic actions.
Diseases of plants.
A group of alicyclic hydrocarbons with the general formula R-C5H9.
A part of the embryo in a seed plant. The number of cotyledons is an important feature in classifying plants. In seeds without an endosperm, they store food which is used in germination. In some plants, they emerge above the soil surface and become the first photosynthetic leaves. (From Concise Dictionary of Biology, 1990)
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
A superfamily of proteins that share a highly conserved MADS domain sequence motif. The term MADS refers to the first four members which were MCM1 PROTEIN; AGAMOUS 1 PROTEIN; DEFICIENS PROTEIN; and SERUM RESPONSE FACTOR. Many MADS domain proteins have been found in species from all eukaryotic kingdoms. They play an important role in development, especially in plants where they have an important role in flower development.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Plant hormones that promote the separation of daughter cells after mitotic division of a parent cell. Frequently they are purine derivatives.
Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.
A plant homeotic protein involved in the development of stamens and carpels of Arabidopsis thaliana. It is a DNA-binding protein that contains the MADS-box domain. It is one of the four founder proteins that structurally define the superfamily of MADS DOMAIN PROTEINS.
A species of gram-negative, fluorescent, phytopathogenic bacteria in the genus PSEUDOMONAS. It is differentiated into approximately 50 pathovars with different plant pathogenicities and host specificities.
Closable openings in the epidermis of plants on the underside of leaves. They allow the exchange of gases between the internal tissues of the plant and the outside atmosphere.
Self-replicating cytoplasmic organelles of plant and algal cells that contain pigments and may synthesize and accumulate various substances. PLASTID GENOMES are used in phylogenetic studies.
The parts of plants, including SEEDS.
Parts of plants that usually grow vertically upwards towards the light and support the leaves, buds, and reproductive structures. (From Concise Dictionary of Biology, 1990)
A class of plant growth hormone isolated from cultures of Gibberella fujikuroi, a fungus causing Bakanae disease in rice. There are many different members of the family as well as mixtures of multiple members; all are diterpenoid acids based on the gibberellane skeleton.
Annual cereal grass of the family POACEAE and its edible starchy grain, rice, which is the staple food of roughly one-half of the world's population.
Substituted thioglucosides. They are found in rapeseed (Brassica campestris) products and related cruciferae. They are metabolized to a variety of toxic products which are most likely the cause of hepatocytic necrosis in animals and humans.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The fertilizing element of plants that contains the male GAMETOPHYTES.
A blue-green biliprotein widely distributed in the plant kingdom.
A plant family of the order Capparales, subclass Dilleniidae, class Magnoliopsida. They are mostly herbaceous plants with peppery-flavored leaves, due to gluconapin (GLUCOSINOLATES) and its hydrolysis product butenylisotrhiocyanate. The family includes many plants of economic importance that have been extensively altered and domesticated by humans. Flowers have 4 petals. Podlike fruits contain a number of seeds. Cress is a general term used for many in the Brassicacea family. Rockcress is usually ARABIS; Bittercress is usually CARDAMINE; Yellowcress is usually RORIPPA; Pennycress is usually THLASPI; Watercress refers to NASTURTIUM; or RORIPPA or TROPAEOLUM; Gardencress refers to LEPIDIUM; Indiancress refers to TROPAEOLUM.
Complex nucleoprotein structures which contain the genomic DNA and are part of the CELL NUCLEUS of PLANTS.
A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; its dried leaves are used for SMOKING.
A plant photo regulatory protein that exists in two forms that are reversibly interconvertible by LIGHT. In response to light it moves to the CELL NUCLEUS and regulates transcription of target genes. Phytochrome B plays an important role in shade avoidance and mediates plant de-etiolation in red light.
The inherent or induced capacity of plants to withstand or ward off biological attack by pathogens.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
The directional growth of organisms in response to gravity. In plants, the main root is positively gravitropic (growing downwards) and a main stem is negatively gravitropic (growing upwards), irrespective of the positions in which they are placed. Plant gravitropism is thought to be controlled by auxin (AUXINS), a plant growth substance. (From Concise Dictionary of Biology, 1990)
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The time period of daily exposure that an organism receives from daylight or artificial light. It is believed that photoperiodic responses may affect the control of energy balance and thermoregulation.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
The absence of light.
A strand of primary conductive plant tissue consisting essentially of XYLEM, PHLOEM, and CAMBIUM.
Plant steroids ubiquitously distributed throughout the plant kingdom. They play essential roles in modulating growth and differentiation of cells at nanomolar to micromolar concentrations.
Geographic variety, population, or race, within a species, that is genetically adapted to a particular habitat. An ecotype typically exhibits phenotypic differences but is capable of interbreeding with other ecotypes.
Porphyrin derivatives containing magnesium that act to convert light energy in photosynthetic organisms.
The unfavorable effect of environmental factors (stressors) on the physiological functions of an organism. Prolonged unresolved physiological stress can affect HOMEOSTASIS of the organism, and may lead to damaging or pathological conditions.
The primary plant photoreceptor responsible for perceiving and mediating responses to far-red light. It is a PROTEIN-SERINE-THREONINE KINASE that is translocated to the CELL NUCLEUS in response to light signals.
The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.
A group of FLAVONOIDS derived from FLAVONOLS, which lack the ketone oxygen at the 4-position. They are glycosylated versions of cyanidin, pelargonidin or delphinidin. The conjugated bonds result in blue, red, and purple colors in flowers of plants.
Prolonged dry periods in natural climate cycle. They are slow-onset phenomena caused by rainfall deficit combined with other predisposing factors.
The capacity of an organism to defend itself against pathological processes or the agents of those processes. This most often involves innate immunity whereby the organism responds to pathogens in a generic way. The term disease resistance is used most frequently when referring to plants.
A cluster of FLOWERS (as opposed to a solitary flower) arranged on a main stem of a plant.
Eukaryotes in the group STRAMENOPILES, formerly considered FUNGI, whose exact taxonomic level is unsettled. Many consider Oomycetes (Oomycota) a phylum in the kingdom Stramenopila, or alternatively, as Pseudofungi in the phylum Heterokonta of the kingdom Chromista. They are morphologically similar to fungi but have no close phylogenetic relationship to them. Oomycetes are found in both fresh and salt water as well as in terrestrial environments. (Alexopoulos et al., Introductory Mycology, 4th ed, pp683-4). They produce flagellated, actively motile spores (zoospores) that are pathogenic to many crop plants and FISHES.
The element in plants that contains the female GAMETOPHYTES.
A plant genus of the family Cruciferae. It contains many species and cultivars used as food including cabbage, cauliflower, broccoli, Brussel sprouts, kale, collard greens, MUSTARD PLANT; (B. alba, B. junica, and B. nigra), turnips (BRASSICA NAPUS) and rapeseed (BRASSICA RAPA).
The ability of organisms to sense and adapt to high concentrations of salt in their growth environment.
The directional growth of organisms in response to light. In plants, aerial shoots usually grow towards light. The phototropic response is thought to be controlled by auxin (= AUXINS), a plant growth substance. (From Concise Dictionary of Biology, 1990)
Any method used for determining the location of and relative distances between genes on a chromosome.
The synthesis by organisms of organic chemical compounds, especially carbohydrates, from carbon dioxide using energy obtained from light rather than from the oxidation of chemical compounds. Photosynthesis comprises two separate processes: the light reactions and the dark reactions. In higher plants; GREEN ALGAE; and CYANOBACTERIA; NADPH and ATP formed by the light reactions drive the dark reactions which result in the fixation of carbon dioxide. (from Oxford Dictionary of Biochemistry and Molecular Biology, 2001)
Steroidal compounds in which one or more carbon atoms in the steroid ring system have been substituted with non-carbon atoms.
A growth from a pollen grain down into the flower style which allows two sperm to pass, one to the ovum within the ovule, and the other to the central cell of the ovule to produce endosperm of SEEDS.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A mitosporic Leotiales fungal genus of plant pathogens. It has teleomorphs in the genus Botryotina.
Membranous cisternae of the CHLOROPLAST containing photosynthetic pigments, reaction centers, and the electron-transport chain. Each thylakoid consists of a flattened sac of membrane enclosing a narrow intra-thylakoid space (Lackie and Dow, Dictionary of Cell Biology, 2nd ed). Individual thylakoids are interconnected and tend to stack to form aggregates called grana. They are found in cyanobacteria and all plants.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Flavoproteins that function as circadian rhythm signaling proteins in ANIMALS and as blue-light photoreceptors in PLANTS. They are structurally-related to DNA PHOTOLYASES and it is believed that both classes of proteins may have originated from an earlier protein that played a role in protecting primitive organisms from the cyclical exposure to UV LIGHT.
Adaptation to a new environment or to a change in the old.
Large and highly vacuolated cells possessing many chloroplasts occuring in the interior cross-section of leaves, juxtaposed between the epidermal layers.
An absence of warmth or heat or a temperature notably below an accustomed norm.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A ubiquitous sodium salt that is commonly used to season food.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Proteins encoded by the CHLOROPLAST GENOME or proteins encoded by the nuclear genome that are imported to and resident in the CHOROPLASTS.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Plant tissue that carries nutrients, especially sucrose, by turgor pressure. Movement is bidirectional, in contrast to XYLEM where it is only upward. Phloem originates and grows outwards from meristematic cells (MERISTEM) in the vascular cambium. P-proteins, a type of LECTINS, are characteristically found in phloem.
A class of amino acids characterized by a closed ring structure.
The failure of PLANTS to complete fertilization and obtain seed (SEEDS) as a result of defective POLLEN or ovules, or other aberrations. (Dict. of Plant Genet. and Mol. Biol., 1998)
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Techniques to alter a gene sequence that result in an inactivated gene, or one in which the expression can be inactivated at a chosen time during development to study the loss of function of a gene.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.
The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
An aminopurine factor in plant extracts that induces cell division. (Grant & Hackh's Chemical Dict, 5th ed)
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A plant species of the family SOLANACEAE, native of South America, widely cultivated for their edible, fleshy, usually red fruit.
Plants that can grow well in soils that have a high SALINITY.
The non-genetic biological changes of an organism in response to challenges in its ENVIRONMENT.
The physiological processes, properties, and states characteristic of plants.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Basic functional unit of plants.
Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.
A plastic substance deposited by insects or obtained from plants. Waxes are esters of various fatty acids with higher, usually monohydric alcohols. The wax of pharmacy is principally yellow wax (beeswax), the material of which honeycomb is made. It consists chiefly of cerotic acid and myricin and is used in making ointments, cerates, etc. (Dorland, 27th ed)
Cholestanes substituted in any position with one or more hydroxy groups. They are found in feces and bile. In contrast to bile acids and salts, they are not reabsorbed.
The reproductive cells of plants.

Separation of shoot and floral identity in Arabidopsis. (1/11716)

The overall morphology of an Arabidopsis plant depends on the behaviour of its meristems. Meristems derived from the shoot apex can develop into either shoots or flowers. The distinction between these alternative fates requires separation between the function of floral meristem identity genes and the function of an antagonistic group of genes, which includes TERMINAL FLOWER 1. We show that the activities of these genes are restricted to separate domains of the shoot apex by different mechanisms. Meristem identity genes, such as LEAFY, APETALA 1 and CAULIFLOWER, prevent TERMINAL FLOWER 1 transcription in floral meristems on the apex periphery. TERMINAL FLOWER 1, in turn, can inhibit the activity of meristem identity genes at the centre of the shoot apex in two ways; first by delaying their upregulation, and second, by preventing the meristem from responding to LEAFY or APETALA 1. We suggest that the wild-type pattern of TERMINAL FLOWER 1 and floral meristem identity gene expression depends on the relative timing of their upregulation.  (+info)

An Arabidopsis 14-3-3 protein can act as a transcriptional activator in yeast. (2/11716)

The 14-3-3 proteins are a group of highly conserved and widely distributed eukaryotic proteins with diverse functions. One 14-3-3 protein, AFT1 from Arabidopsis thaliana, was found to be able to activate transcription in yeast. When fused to the DNA-binding domain of a bacterial protein LexA, AFT1 can activate transcription of reporter genes that contain LexA operator sequences in their promoters. Although the in vivo function of AFT1 is not completely known, its similarity to previously identified proteins found in transcription complexes of Arabidopsis and maize suggests that AFT1 and some other 14-3-3 proteins may activate gene expression in other systems as well.  (+info)

RAD53 regulates DBF4 independently of checkpoint function in Saccharomyces cerevisiae. (3/11716)

The Cdc7p and Dbf4p proteins form an active kinase complex in Saccharomyces cerevisiae that is essential for the initiation of DNA replication. A genetic screen for mutations that are lethal in combination with cdc7-1 led to the isolation of seven lsd (lethal with seven defect) complementation groups. The lsd7 complementation group contained two temperature-sensitive dbf4 alleles. The lsd1 complementation group contained a new allele of RAD53, which was designated rad53-31. RAD53 encodes an essential protein kinase that is required for the activation of DNA damage and DNA replication checkpoint pathways, and that is implicated as a positive regulator of S phase. Unlike other RAD53 alleles, we demonstrate that the rad53-31 allele retains an intact checkpoint function. Thus, the checkpoint function and the DNA replication function of RAD53 can be functionally separated. The activation of DNA replication through RAD53 most likely occurs through DBF4. Two-hybrid analysis indicates that the Rad53p protein binds to Dbf4p. Furthermore, the steady-state level of DBF4 message and Dbf4p protein is reduced in several rad53 mutant strains, indicating that RAD53 positively regulates DBF4. These results suggest that two different functions of the cell cycle, initiation of DNA replication and the checkpoint function, can be coordinately regulated through the common intermediate RAD53.  (+info)

Overexpression of a novel Arabidopsis gene related to putative zinc-transporter genes from animals can lead to enhanced zinc resistance and accumulation. (4/11716)

We describe the isolation of an Arabidopsis gene that is closely related to the animal ZnT genes (Zn transporter). The protein encoded by the ZAT (Zn transporter of Arabidopsis thaliana) gene has 398 amino acid residues and is predicted to have six membrane-spanning domains. To obtain evidence for the postulated function of the Arabidopsis gene, transgenic plants with the ZAT coding sequence under control of the cauliflower mosaic virus 35S promoter were analyzed. Plants obtained with ZAT in the sense orientation exhibited enhanced Zn resistance and strongly increased Zn content in the roots under high Zn exposure. Antisense mRNA-producing plants were viable, with a wild-type level of Zn resistance and content, like plants expressing a truncated coding sequence lacking the C-terminal cytoplasmic domain of the protein. The availability of ZAT can lead to a better understanding of the mechanism of Zn homeostasis and resistance in plants.  (+info)

Discrete domains mediate the light-responsive nuclear and cytoplasmic localization of Arabidopsis COP1. (5/11716)

The Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) protein plays a critical role in the repression of photomorphogenesis during Arabidopsis seedling development. We investigated the control of COP1 partitioning between nucleus and cytoplasm, which has been implicated in the regulation of COP1 activity, by using fusion proteins between COP1 and beta-glucuronidase or the green fluorescent protein. Transient expression assays using onion epidermal cells and data from hypocotyl cells of stably transformed Arabidopsis demonstrated that COP1 carries a single, bipartite nuclear localization signal that functions independently of light. Nuclear exclusion was mediated by a novel and distinct signal, bordering the zinc-finger and coiled-coil motifs, that was able to redirect a heterologous nuclear protein to the cytoplasm. The cytoplasmic localization signal functioned in a light-independent manner. Light regulation of nuclear localization was reconstituted by combining the individual domains containing the nuclear localization signal and the cytoplasmic localization signal; the WD-40 repeat domain of COP1 was not required. However, phenotypic analysis of transgenic seedlings suggested that the constitutively nuclear-localized WD-40 repeat domain was able to mimic aspects of COP1 function, as indicated by exaggerated hypocotyl elongation under light conditions.  (+info)

IAR3 encodes an auxin conjugate hydrolase from Arabidopsis. (6/11716)

Amide-linked conjugates of indole-3-acetic acid (IAA) are putative storage or inactivation forms of the growth hormone auxin. Here, we describe the Arabidopsis iar3 mutant that displays reduced sensitivity to IAA-Ala. IAR3 is a member of a family of Arabidopsis genes related to the previously isolated ILR1 gene, which encodes an IAA-amino acid hydrolase selective for IAA-Leu and IAA-Phe. IAR3 and the very similar ILL5 gene are closely linked on chromosome 1 and comprise a subfamily of the six Arabidopsis IAA-conjugate hydrolases. The purified IAR3 enzyme hydrolyzes IAA-Ala in vitro. iar 3 ilr1 double mutants are more resistant than either single mutant to IAA-amino acid conjugates, and plants overexpressing IAR3 or ILR1 are more sensitive than is the wild type to certain IAA-amino acid conjugates, reflecting the overlapping substrate specificities of the corresponding enzymes. The IAR3 gene is expressed most strongly in roots, stems, and flowers, suggesting roles for IAA-conjugate hydrolysis in those tissues.  (+info)

The CLAVATA1 receptor-like kinase requires CLAVATA3 for its assembly into a signaling complex that includes KAPP and a Rho-related protein. (7/11716)

The CLAVATA1 (CLV1) and CLAVATA3 (CLV3) genes are required to maintain the balance between cell proliferation and organ formation at the Arabidopsis shoot and flower meristems. CLV1 encodes a receptor-like protein kinase. We have found that CLV1 is present in two protein complexes in vivo. One is approximately 185 kD, and the other is approximately 450 kD. In each complex, CLV1 is part of a disulfide-linked multimer of approximately 185 kD. The 450-kD complex contains the protein phosphatase KAPP, which is a negative regulator of CLV1 signaling, and a Rho GTPase-related protein. In clv1 and clv3 mutants, CLV1 is found primarily in the 185-kD complex. We propose that CLV1 is present as an inactive disulfide-linked heterodimer and that CLV3 functions to promote the assembly of the active 450-kD complex, which then relays signal transduction through a Rho GTPase.  (+info)

Mutations in FIE, a WD polycomb group gene, allow endosperm development without fertilization. (8/11716)

A fundamental problem in biology is to understand how fertilization initiates reproductive development. Higher plant reproduction is unique because two fertilization events are required for sexual reproduction. First, a sperm must fuse with the egg to form an embryo. A second sperm must then fuse with the adjacent central cell nucleus that replicates to form an endosperm, which is the support tissue required for embryo and/or seedling development. Here, we report cloning of the Arabidopsis FERTILIZATION-INDEPENDENT ENDOSPERM (FIE) gene. The FIE protein is a homolog of the WD motif-containing Polycomb proteins from Drosophila and mammals. These proteins function as repressors of homeotic genes. A female gametophyte with a loss-of-function allele of fie undergoes replication of the central cell nucleus and initiates endosperm development without fertilization. These results suggest that the FIE Polycomb protein functions to suppress a critical aspect of early plant reproduction, namely, endosperm development, until fertilization occurs.  (+info)

New DNA Sequences ======================= AC013430 AC013430 88172bp DNA HTG 11-NOV-1999 Arabidopsis thaliana chromosome 1 clone F3F9, WORKING DRAFT SEQUENCE, 6 unordered pieces. HTG; HTGS_PHASE1. ATF11C1 AL132976 105644bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F11C1 ATF15G16 AL132959 104868bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F15G16 ATF18N11 AL132953 91275bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F18N11 ATF1P2 AL132955 101154bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F1P2 ATF24B22 AL132957 100285bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F24B22 ATF24M12 AL132980 129515bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F24M12 ATF2A19 AL132962 95993bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F2A19 ATF2K15 AL132956 130956bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F2K15 ...
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Here, we report our effort in generating an ORFeome collection for the Arabidopsis transcription factor (TF) genes. In total, ORFeome clones representing 1,282 Arabidopsis TF genes have been obtained in the Gateway high throughput cloning pENTR vector, including 411 genes whose annotation lack cDNA support. All the ORFeome inserts have also been mobilized into a yeast expression destination vector, with an estimated 85% rate of expressing the respective proteins. Sequence analysis of these clones revealed that 34 of them did not match with either the reported cDNAs or current predicted open-reading-frame sequences. Among those, novel alternative splicing of TF gene transcripts is responsible for the observed differences in at least five genes. However, those alternative splicing events do not appear to be differentially regulated among distinct Arabidopsis tissues examined. Lastly, expression of those TF genes in 17 distinct Arabidopsis organ types and the cultured cells was profiled using a ...
Studies on epidermal cell fate determination have been important for gaining insight into the genetic and molecular mechanisms leading to the differentiation and patterning of cells. In Arabidopsis, the organization and development of many epidermal characters including trichomes, root hairs and the seed coat have been found to be controlled by a single combinatorial transcription factor complex consisting of a WD-repeat containing protein, Transparent Testa Glabra 1 (TTG1), and various MYB and bHLH proteins. The work here consists of identification of Glabra2 (GL2) and Transparent Testa Glabra2 (TTG2) as direct transcriptional targets of the TTG1 combinatorial complex, further characterization of GL2 function, and identification of transcriptional targets of GL2 and TTG2. Both GL2 and TTG2 are important in the regulation of trichomes, root hairs and seed coat development. vii GL2 has been identified as an important regulator of epidermal cell fate for over fifteen years yet there is little ...
The Arabidopsis pseudo-response regulator, APRR1, has a unique structural design containing a pseudo-receiver domain and a C-terminal CONSTANS motif. This protein was originally characterized as a presumed component of the His-to-Asp phosphorelay systems in Arabidopsis thaliana. Recently, it was reported that APRR1 is identical to the TOC1 gene product, a mutational lesion of which affects the periods of many circadian rhythms in Arabidopsis plants. TOC1 is believed to be a component of the presumed circadian clock (or central oscillator). Based on these facts, in this study four more genes, each encoding a member of the APRR1/TOC1 family of pseudo-response regulators were identified and characterized with special reference to circadian rhythms. It was found that all these members of the APRR1/TOC1 family (APRR1, APRR3, APRR5, APRR7, and APRR9) are subjected to a circadian rhythm at the level of transcription. Furthermore, in a given 24 h period, the APRR-mRNAs started accumulating sequentially ...
Dear Colleagues, JASPAR ( is the open access reference database for transcription factor (TF) DNA binding profiles. Its used in major projects such as ENCODE or FANTOM or tools such as the MEME suite. Plants are underrepresented in the current Jaspar version and I am currently collecting information to include as many Arabidopsis TF as possible in the next Jaspar release. This effort will be useful for the Arabidopsis community as many tools using Jaspar will become available for Arabidopsis in the future if the database is populated with Arabidopsis TF. The TF specificity can be derived from ChIP-chip, ChIP-Seq, Selex/BSSA, Protein binding microarray. I have collected from the literature but I might have missed some factors. If you have generated such data in the past, or if such data exists for TF youre working on, it would be very helpful if you could send me a short mail indicating the TF name and the reference. You can also share unpublished ChIP or Selex data ...
Our observation that both Si1 and Zmm16 are sufficient to rescue their corresponding Arabidopsis mutants also provides compelling evidence for the conservation of B-class function. It would be expected, if B-class genes had evolved significantly different roles in either maize or Arabidopsis, that the maize genes would not be sufficient to functionally replace the Arabidopsis genes. However, our results indicate that the maize genes, either in combination with their respective Arabidopsis B-class protein partners, or together in Arabidopsis ap3 pi double mutants, are capable of correctly regulating the downstream targets necessary for stamen and petal development, even though in maize, B-class activity is essential for promoting stamen and lodicule development.. It is important to note that the rescue seen in the two strong lines examined was correlated with higher levels of expression than that of the Arabidopsis orthologs. Consequently, it may be necessary to have higher levels of the maize ...
The availability of the complete genome sequence of Arabidopsis thaliana together with those of other organisms provides an opportunity to decipher the genetic factors that define plant form and function. To begin this task, we have classified the nuclear protein-coding genes of Arabidopsis thaliana on the basis of their pattern of sequence similarity to organisms across the three domains of life. We identified 3,848 Arabidopsis proteins that are likely to be found solely within the plant lineage. More than half of these plant-specific proteins are of unknown function, emphasizing the general lack of knowledge of processes unique to plants. Plant-specific proteins that are membrane-associated and/or targeted to the mitochondria or chloroplasts are the most poorly characterized. Analyses of microarray data indicate that genes coding for plant-specific proteins, but not evolutionarily conserved proteins, are more likely to be expressed in an organ-specific manner. A large proportion (13%) of plant
Also known as: IN Arabidopsis, Arabidopsis., Arabidopsis At, Arabidopsis AS, Arabidopsis, Ara-bidopsis, ARABIDOPSIS In, ARABIDOPSIS ...
Despite the importance of secondary growth in plants, relatively few genes regulating this process have been identified to date. By using data from detailed transcript profiling of the poplar wood-forming tissues, 150 genes that are differentially expressed within the zone of secondary growth were identified. In order to determine the possible function of these poplar genes, potential Arabidopsis thaliana orthologs were identified and gene knockout lines analysed. Three selection filters were used to identify the most likely orthologous genes using poplar and Arabidopsis sequence comparisons, expression profiling in secondary thickened Arabidopsis hypocotyls and global expression analysis of Arabidopsis tissues. Three genes encoding AtCSLA2 (At5g22740), the AtGUT1 GT47 glycosyltransferase (At1g27440) and a protein with no proposed function AtUNKA (At4g27435) were selected for further detailed analysis of their role in secondary growth in Arabidopsis. The presented genome-based approach using ...
T-DNA Express: Arabidopsis Gene Mapping Tool, Arabidopsis Functional Genomics Database. gebd Arabidopsis Genome Browser. iSect Tools, iView Tools and Gene Expression Atlas. Collection of Arabidopsis T-DNA/Ds, Full-length cDNA, Arabidopsis Gene Expression Database. Created and developed by Huaming Chen
T-DNA Express: Arabidopsis Gene Mapping Tool, Arabidopsis Functional Genomics Database. gebd Arabidopsis Genome Browser. iSect Tools, iView Tools and Gene Expression Atlas. Collection of Arabidopsis T-DNA/Ds, Full-length cDNA, Arabidopsis Gene Expression Database. Created and developed by Huaming Chen
T-DNA Express: Arabidopsis Gene Mapping Tool, Arabidopsis Functional Genomics Database. gebd Arabidopsis Genome Browser. iSect Tools, iView Tools and Gene Expression Atlas. Collection of Arabidopsis T-DNA/Ds, Full-length cDNA, Arabidopsis Gene Expression Database. Created and developed by Huaming Chen
The WD motif (also known as the Trp-Asp or WD40 motif) is found in a multitude of eukaryotic proteins involved in a variety of cellular processes. Where studied, repeated WD motifs act as a site for protein-protein interaction, and proteins containing WD repeats (WDRs) are known to serve as platforms for the assembly of protein complexes or mediators of transient interplay among other proteins. In the model plant Arabidopsis thaliana, members of this superfamily are increasingly being recognized as key regulators of plant-specific developmental events. We analyzed the predicted complement of WDR proteins from Arabidopsis, and compared this to those from budding yeast, fruit fly and human to illustrate both conservation and divergence in structure and function. This analysis identified 237 potential Arabidopsis proteins containing four or more recognizable copies of the motif. These were classified into 143 distinct families, 49 of which contained more than one Arabidopsis member. Approximately 113 of
TY - JOUR. T1 - BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. AU - Li, Jia. AU - Wen, Jiangqi. AU - Lease, Kevin A.. AU - Doke, Jason T.. AU - Tax, Frans E.. AU - Walker, John C.. N1 - Funding Information: We thank S. Zhang, B. Sonderman, members of the University of Missouri-Columbia Molecular Cytology Core Facility (J. Wagner, S. Bailes, and M. Sivaguru), and members of the University of Wisconsin Arabidopsis knockout facility for their excellent technical assistance. We also thank members of the Walker laboratory for aid and discussion. This work was supported by National Science Foundation Grants MCB 9809884, MCB 0112278, DBI 9975808 (J.C.W.), and IBN 0132554 (J.L. and J.C.W.) and by the University of Missouri Food for the 21 st Century Program.. PY - 2002/7/26. Y1 - 2002/7/26. N2 - Brassinosteroids regulate plant growth and development through a protein complex that includes the leucine-rich repeat receptor-like protein ...
A large number of disease resistance genes or QTLs in crop plants are identified through conventional genetics and genomic tools, but their functional or molecular characterization remains costly, labor-intensive and inaccurate largely due to the lack of deep sequencing of large and complex genomes of many important crops such as allohexaploid wheat (Triticum aestivum L.). On the other hand, gene annotation and relevant genomic resources for disease resistance and other defense-related traits are more abundant in model plant Arabidopsis (Arabidopsis thaliana). The objectives of this study are (i) to infer homology of defense-related genes in Arabidopsis and wheat and (ii) to classify these homologous genes into different gene families. We employed three bioinformatics and genomics approaches to identifying candidate genes known to affect plant defense and to classifying these protein-coding genes into different gene families in Arabidopsis. These approaches predicted up to 1790 candidate genes in 11
Plant parasitic nematodes, including root-knot nematodes and cyst nematodes, cause extensive damage and loss to many crops. Nematodes elicit a…
With the set of Arabidopsis plasma membrane phosphorylation sites presented in this work, we have made two major achievements. First, as a database resource, the data will facilitate signaling research on the proteins identified. Technically, it remains difficult, if not impossible, for most laboratories to identify phosphorylation sites within their proteins of interest. These data, and those that follow, should accelerate studies into the mechanistic regulation of biological processes in plants in a manner comparable to the way genome and EST sequencing projects have facilitated the cloning of genes of interest. All data will be released as a free access, searchable database through PlantsP ( Second, in a wider scope, the data provide insight into general emerging patterns of regulation through phosphorylation as well as an appreciation of the complexity of these processes.. It is important to stress that the identification of phosphorylation sites as described in this ...
TY - JOUR. T1 - Arabidopsis plasma membrane proteomics identifies components of transport, signal transduction and membrane trafficking. AU - Alexandersson, E.. AU - Saalbach, G.. AU - Larsson, C.. AU - Kjellbom, P.. PY - 2004. Y1 - 2004. KW - 8-B gen. U2 - 10.1093/pcp/pch209. DO - 10.1093/pcp/pch209. M3 - Journal article. C2 - 15574830. VL - 45. SP - 1543. EP - 1556. JO - Plant Cell Physiol.. JF - Plant Cell Physiol.. ER - ...
In most regions and when doing a comparison of the whole genomes, Arabidopsis shows a great affinity for mononucleotide repeats compared to rice (Figures 1 to 5). The comparison between the whole genomes of rice and Arabidopsis clearly shows that Arabidopsis has a higher percentage of mononucleotide repeats (33.9%) than rice (18.3%). The only regions where mononucleotide repeats are not as high are the 5UTR and coding regions. Rice seems to have a greater affinity for trinucleotide repeats, with an exceptionally high density of approximately 1,670/MB in the 5UTR, even higher than in exon regions (approximately 421.4/MB). In fact, trinucleotide SSRs are the major type of SSR in all regions except 3UTRs (Figures 1 to 5).. Yet despite these differences in which type of SSR is most common for each organism, rice and Arabidopsis show similar distribution of the SSR types (period) in their coding regions. Both have a majority of trinucleotide repeats (Arabidopsis 65.4%, rice 64%) and a lack of any ...
Gene expression databases for Arabidopsis and other organisms are important resources to investigate the expression pattern of a given gene or gene families, to identify genes that respond to specific stimuli, and to search for coexpressed genes (;; Steinhauser et al., 2004; Zimmermann et al., 2004; Shen et al., 2005). AtGenExpress is a very recent gene expression database for Arabidopsis, and possibly the most standardized and comprehensive gene expression resource for any multicellular organism (Altmann et al., 2004). We complemented this dataset with our own data (see introduction) and used the resulting set of Affymetrix Arabidopsis ATH1 whole-genome GeneChip data (approximately 16 million data points i.e. 721 arrays or 323 conditions) to investigate the expression stability of traditional reference genes and to identify novel Arabidopsis reference genes (see Supplemental Table I) that outperform the traditional ones in terms ...
According to the prediction of the involvement of bZIP transcription factors in the ER stress response, AtbZIP60 was identified by genome-wide screening based on genomic information on Arabidopsis. Tunicamycin and other reagents activating the ER stress response induced transcripts of AtbZIP60. From these results, we predicted that AtbZIP60 plays a role in the ER stress response. Because the expression profile of AtbZIP60 was close to that of BiP, induction of AtbZIP60 transcript was not considered to be the first trigger of activation for BiP expression. Instead, it was assumed that a conformational change of AtbZIP60 activates the expression of chaperone genes, such as BiP. This prediction was based on the fact that AtbZIP60 contains a putative TMD like that of ATF6 in mammalian cells. Specifically, it was hypothesized that AtbZIP60 is converted to a soluble form by ER stress and becomes localized to the nucleus, resulting in the activation of chaperone genes. Indeed, a truncated form of ...
Transmembrane leucine-rich repeat (LRR) receptors are commonly used innate immune receptors in plants and animals but can also sense endogenous signals to regulate development. BAK1 is a plant LRR-receptor-like kinase (RLK) that interacts with several ligand-binding LRR-RLKs to positively regulate their functions. BAK1 is involved in brassinosteroid-dependent growth and development, innate immunity, and cell-death control by interacting with the brassinosteroid receptor BRI1, immune receptors, such as FLS2 and EFR, and the small receptor kinase BIR1, respectively.. ...
Cyclome: Arabidopsis Cyclome Functional Genomics Database. gebd Arabidopsis Genome Browser. iSect Tools, iView Tools and Gene Expression Atlas. Collection of Arabidopsis T-DNA/Ds, Full-length cDNA, Marker, EST, MPSS, SAGE, miRNA, sRNA, Arabidopsis Tiling Array and Gene Expression Data. Created and developed by Huaming Chen
The flowering plant Arabidopsis thaliana is an important model system for identifying genes and determining their functions. Here we report the analysis of the genomic sequence of Arabidopsis. The sequenced regions cover 115.4 megabases of the 125-megabase genome and extend into centromeric regions. …
TY - JOUR. T1 - Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. AU - Kaul, Samir. AU - Koo, Hean L.. AU - Jenkins, Jennifer. AU - Rizzo, Michael. AU - Rooney, Timothy. AU - Tallon, Luke J.. AU - Feldblyum, Tamara. AU - Nierman, William. AU - Benito, Maria Ines. AU - Lin, Xiaoying. AU - Town, Christopher D.. AU - Venter, J. Craig. AU - Fraser, Claire M.. AU - Tabata, Satoshi. AU - Nakamura, Yasukazu. AU - Kaneko, Takakazu. AU - Sato, Shusei. AU - Asamizu, Erika. AU - Kato, Tomohiko. AU - Kotani, Hirokazu. AU - Sasamoto, Shigemi. AU - Ecker, Joseph R.. AU - Theologis, Athanasios. AU - Federspiel, Nancy A.. AU - Palm, Curtis J.. AU - Osborne, Brian I.. AU - Shinn, Paul. AU - Dewar, Ken. AU - Kim, Christopher J.. AU - Buehler, Eugen. AU - Dunn, Patrick. AU - Chao, Qimin. AU - Chen, Huaming. AU - Theologis, Athanasios. AU - Osborne, Brian I.. AU - Vysotskaia, Valentina S.. AU - Lenz, Catherine A.. AU - Kim, Christopher J.. AU - Hansen, Nancy F.. AU - Liu, Shirley ...
Description of disease Arabidopsis thaliana. Treatment Arabidopsis thaliana. Symptoms and causes Arabidopsis thaliana Prophylaxis Arabidopsis thaliana
TY - JOUR. T1 - Formate dehydrogenase in Arabidopsis thaliana. T2 - Overexpression and subcellular localization in leaves. AU - Herman, Patricia L.. AU - Ramberg, Håkon. AU - Baack, Renee D.. AU - Markwell, John. AU - Osterman, John C.. PY - 2002/12/1. Y1 - 2002/12/1. N2 - Formate dehydrogenase (FDH; EC is a NAD-dependent enzyme that catalyzes the oxidation of formate to carbon dioxide in the mitochondria of higher plants. Sequence analyses and other preliminary experiments suggested that FDH might also be targeted to the chloroplasts of Arabidopsis thaliana and other plant species. In the present study, transgenic Arabidopsis and tobacco plants that overexpress Arabidopsis FDH were produced. The FDH specific activity in the leaf tissue of the transgenic plants increased an average of 4.5-fold for Arabidopsis and 31.5-fold for tobacco. Immunodetection and enzyme assays of intact chloroplasts fractionated from the leaves of transgenic tobacco plants suggested that Arabidopsis FDH is ...
The TRANSPARENT TESTA GLABRA1 (TTG1) locus controls many apparently unrelated characters of Arabidopsis (catalogued by Koornneef, 1981), several of which appear to be confined to the epidermal cell layer of different tissues. ttg1 mutants have a glabrous phenotype, possessing none of the leaf or stem hairs (trichomes) that normally are derived from the meristematic L1 cell layer. Purple anthocyanin pigments are absent from the ttg1 seed coat, causing the transparent testa phenotype in which the yellow cotyledons are visible through the testa. In wild-type plants, anthocyanins are present in the hypocotyl of seedlings and in the stem and leaves of plants as they age, and they are inducible by many forms of stress, including high light, poor nutrients, or water stress. ttg1 mutants completely lack anthocyanins in the epidermis and in subepidermal layers of leaves and stems. Mucilage normally found in the cell wall of the seed coat is absent in ttg1 mutants. Seeds of ttg1 plants do not require ... Arabidopsis thaliana (Mouse-ear cress) is a flowering plant belonging to the family Brassicaceae which contains economically important brassica and mustard species. Arabidopsis thaliana was the first plant to have its genome sequenced. Arabidopsis thaliana is not of economic value itself, but has risen to prominence because of its small size, short generation time and small genome, which make it an ideal plant to use for research. The Arabidopsis thaliana genome has a haploid chromosome number of 5, containing 135 Mb with 32,000 protein-coding genes. The reference proteome is derived from the genome sequence published in 2000 for the ecotype Columbia ( ...
Jasmonic acid and its methyl ester, methyl jasmonate (MeJA), are plant signaling molecules that affect plant growth and gene expression. Primary root growth of wild-type Arabidopsis thaliana seedlings was inhibited 50% when seedlings were grown on agar medium containing 0.1 M MeJA. An ethyl methanesulfonate mutant (jar1) with decreased sensitivity to MeJA inhibition of root elongation was isolated and characterized. Genetic data indicated the trait was recessive and controlled by a single Mendelian factor. MeJA-induced polypeptides were detected in Arabidopsis leaves by antiserum to a MeJA-inducible vegetative storage protein from soybean. The induction of these proteins by MeJA in the mutant was at least 4-fold less in jar1 compared to wild type. In contrast, seeds of jar1 plants were more sensitive than wild type to inhibition of germination by abscisic acid. These results suggest that the defect in jar1 affects a general jasmonate response pathway, which may regulate multiple genes in ...
Zea mays MATH-BTB protein (ZmMAB1) has been shown to have a role in regulation and proper asimetric cell divisions during the male and female gametophyte development. Its role has been demonstrated in proteasomal degradation as part of a ubiquitin E3 ligase complex. ZmMAB1 gene expression in Zea mays is precisely regulated, and the protein product is a short-lived protein. Therefore ZmMAB1 gene expression research in a homolougus system is difficult. Cullin is a structural component of many E3 ligases. It has been shown that Cul3a protein from Arabidopsis thaliana (L.) Heynh. forms complexes with MAB1 from Zea mays, so Arabidopsis thaliana has been chosen for expression regulation research of MAB1 in a heterologous system. For the cause of better understanding the role and regulation of ZmMAB1 protein, GFP florescent protein labeled ZmMAB1 protein has been inserted in the genome of A. thaliana with bacterium Agrobacterium tumefaciens. Goal of this thesis was to determine on which level of ...
Seeds of Arabidopsis contain ~40% of triacylglycerol. It is converted to sugar to support post germination growth. We identified an Arabidopsis T-DNA knockout mutant that was sugar dependent during early seedling establishment. Our study showed that the β-oxidation process involved in catabolising the free fatty acids released from the seed triacylglycerol was impaired in this mutant. This mutant was confirmed to be transcriptional null for the Protein Acyl Transferase 15, AtPAT15 (At5g04270), one of the 24 protein acyl transferases in Arabidopsis. Although it is the shortest AtPAT15 contains the signature Asp-His-His-Cys cysteine rich domain which is essential for the enzyme activity of this family of proteins. The function of AtPAT15 was validated because it rescued the growth defect of the yeast protein acyl transferase mutant akr1 and it was also auto-acylated in vitro. Transient expression of AtPAT15 in Arabidopsis and tobacco localized AtPAT15 in the Golgi apparatus. Taken together, our ...
Plants are exposed to many environmental stresses that affect their growth and development. These stresses include biotic stresses (via organisms) and abiotic stresses (via environment). Plants respond to these stresses by transcriptional reprogramming and different signaling pathways. Arabidopsis thaliana has shown great sensitivity to the biotic stress Botrytis cinerea. The WRKY33 gene plays an important role in plant defense mechanism against this pathogen. The overall goal is to identify common regulated genes of wrky33 mutant and 35S:WRKY33 over expressing transgenic lines in response to B. cinerea; ultimately to improve plant stress tolerance in Arabidopsis. The specific aims are to: (1) identify Arabidopsis regulated genes in response to B. cinerea infection; (2) determine common up- and down-regulated genes in response to B. cinerea infection in Arabidopsis wrky33 mutant and 35S:WRKY33 overexpression lines; and (3) characterize the function of the common genes in response to B. cinerea. To
In the year 2000, the first complete nuclear genome of a plant species - Arabidopsis thaliana - was released into the wild (a.k.a to bunch of salivating scientists). Less than twenty years later, we had a total of 1135 genomes… for Arabidopsis alone! Today were talking about Arabidopsis races, and how they are a powerful tool for unravelling plant secrets.. Arabidopsis likely diverged from its closest relative about 10 million years ago, and the invasive weed has since spread through Northern Eurasia. In the literally millions of years following, the species diversified into hundreds of race-like ecotypes: populations of plants that have settled in, and then adapted to, certain geological areas. While there has been some cross-talk (i.e, cross breeding) between the ecotypes, there has also been a whole lot of time spent alone - leading to evolution of traits that can differ from one ecotype to the next. Arabidopsis ecotypes (also called accessions) can differ from each other in their size, ...
The phytohormone gibberellin (GA) regulates the development and fertility of Arabidopsis flowers. The mature flowers of GA-deficient mutant plants typically exhibit reduced elongation growth of petals and stamens. In addition, GA-deficiency blocks anther development, resulting in male sterility. Previous analyses have shown that GA promotes the elongation of plant organs by opposing the function of the DELLA proteins, a family of nuclear growth repressors. However, it was not clear that the DELLA proteins are involved in the GA-regulation of stamen and anther development. We show that GA regulates cell elongation rather than cell division during Arabidopsis stamen filament elongation. In addition, GA regulates the cellular developmental pathway of anthers leading from microspore to mature pollen grain. Genetic analysis shows that the Arabidopsis DELLA proteins RGA and RGL2 jointly repress petal, stamen and anther development in GA-deficient plants, and that this function is enhanced by RGL1 ...
Arabidopsis thaliana + , Arabidopsis thaliana (L.) Heynh. + , Arabidopsis thaliana (thale cress) + , Arbisopsis thaliana + , mouse-ear cress + , thale cress + , thale-cress + ...
Arabidopsis thaliana (Arabidopsis) Col-0 was inoculated with Phytophthora cinnamomi to assess the interaction and defence responses involved. Pathogen ingress and asexual reproduction occurred on root tissue but not leaf tissue. The colonisation of root tissue did not cause disease symptoms or plant death, indicating that Arabidopsis Col-0 was tolerant of the infection. The induction of several plant defence responses including the expression of defence-related genes were found, with differences displayed between inoculated root and leaf tissue. Arabidopsis defence-related gene mutant/over-expressing lines were also inoculated with P. cinnamomi but none of the lines tested exhibited a marked increase in susceptibility to the pathogen ...
Shop ARMADILLO BTB ARABIDOPSIS protein ELISA Kit, Recombinant Protein and ARMADILLO BTB ARABIDOPSIS protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Mitochondria depend on a nucleus-encoded transcription machinery to express their genome. The present study examined the transcription of mitochondrial genes by two nucleus-encoded phage-type RNA polymerases, RpoTm and RpoTmp, in the plant Arabidopsis. For selected mitochondrial genes in Arabidopsis, transcription initiation sites were determined. Most genes were found to possess multiple promoters. The identified promoters displayed diverse sequence elements and mostly deviated from a nonanucleotide consensus derived previously for dicot mitochondrial promoters. Several promoters were detected that activate transcription of presumably non-functional sequences. Promoter architecture, distribution and utilization suggest a non-stringent control of transcription initiation in Arabidopsis mitochondria. An in vitro transcription system was set up to elucidate the roles of RpoTm and RpoTmp. Since RpoT enzymes possibly require auxiliary factors, the Arabidopsis genome was screened for potential ...
Most legume plants can form nodules, specialized lateral organs that form on roots, and house nitrogen-fixing bacteria collectively called rhizobia. The uptake of the phytohormone auxin into cells is known to be crucial for development of lateral roots. To test the role of auxin influx in nodulation we used the auxin influx inhibitors 1-naphthoxyacetic acid (1-NOA) and 2-NOA, which we found reduced nodulation of Medicago truncatula. This suggested the possible involvement of the AUX/LAX family of auxin influx transporters in nodulation. Gene expression studies identified MtLAX2, a paralogue of Arabidopsis (Arabidopsis thaliana) AUX1, as being induced at early stages of nodule development. MtLAX2 is expressed in nodule primordia, the vasculature of developing nodules, and at the apex of mature nodules. The MtLAX2 promoter contains several auxin response elements, and treatment with indole-acetic acid strongly induces MtLAX2 expression in roots. mtlax2 mutants displayed root phenotypes similar to ...
EN] The plant endosomal trafficking pathway controls the abundance of membrane-associated soluble proteins, as shown for abscisic acid (ABA) receptors of the PYRABACTIN RESISTANCE1/PYR1-LIKE/REGULATORY COMPONENTS OF ABA RECEPTORS (PYR/PYL/RCAR) family. ABA receptor targeting for vacuolar degradation occurs through the late endosome route and depends on FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING1 (FYVE1) and VACUOLAR PROTEIN SORTING23A (VPS23A), components of the ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT-I (ESCRT-I) complexes. FYVE1 and VPS23A interact with ALG-2 INTERACTING PROTEIN-X (ALIX), an ESCRT-III-associated protein, although the functional relevance of such interactions and their consequences in cargo sorting are unknown. In this study we show that Arabidopsis (Arabidopsis thaliana) ALIX directly binds to ABA receptors in late endosomes, promoting their degradation. Impaired ALIX function leads to altered endosomal localization and increased accumulation of ABA receptors. ...
The Arabidopsis Information Resource (TAIR, is the model organism database for the fully sequenced and intensively studied model plant Arabidopsis thaliana. Data in TAIR is derived in large part from manual curation of the Arabidopsis research literature and direct submission …
We generated fusions between three Arabidopsis (Arabidopsis thaliana) tonoplast intrinsic proteins (TIPs; alpha-, gamma-, and delta-TIP) and yellow fluorescent protein (YFP). We also produced soluble reporters consisting of the monomeric red fluorescent protein (RFP) and either the C-terminal vacuolar sorting signal of phaseolin or the sequence-specific sorting signal of proricin. In transgenic Arabidopsis leaves, mature roots, and root tips, all TIP fusions localized to the tonoplast of the central vacuole and both of the lumenal RFP reporters were found within TIP-delimited vacuoles. In embryos from developing, mature, and germinating seeds, all three TIPs localized to the tonoplast of protein storage vacuoles. To determine the temporal TIP expression patterns and to rule out mistargeting due to overexpression, we generated plants expressing YFP fused to the complete genomic sequences of the three TIP isoforms. In transgenic Arabidopsis, gamma-TIP expression was limited to vegetative tissues, ...
We report here an efficient method for targeted mutagenesis of Arabidopsis genes through regulated expression of zinc finger nucleases (ZFNs)-enzymes engineered to create DNA double-strand breaks at specific target loci. ZFNs recognizing the Arabidopsis ADH1 and TT4 genes were made by Oligomerized Pool ENgineering (OPEN)-a publicly available, selection-based platform that yields high quality zinc finger arrays. The ADH1 and TT4 ZFNs were placed under control of an estrogen-inducible promoter and introduced into Arabidopsis plants by floral-dip transformation. Primary transgenic Arabidopsis seedlings induced to express the ADH1 or TT4 ZFNs exhibited somatic mutation frequencies of 7% or 16%, respectively. The induced mutations were typically insertions or deletions (1-142 bp) that were localized at the ZFN cleavage site and likely derived from imprecise repair of chromosome breaks by nonhomologous end-joining. Mutations were transmitted to the next generation for 69% of primary transgenics expressing the
A prime example of across organisms and borderless scientific activities in IIGB was achieved by Thomas Eulgem and Karine Le Roch, with a well-executed collaboration bringing together researchers working in very different areas of genome biology. The project was initiated in Thomas Eulgems lab as the PI on the critical roles of the chromatin-associated Arabidopsis thaliana protein EDM2 in coordinating plant immune responses. Karine Le Rochs group contributed expertise and experience on epigenome profiling to the study.. The PLOS Genetics paper, The Arabidopsis PHD-finger protein EDM2 has multiple roles in balancing NLR immune receptor gene expression, can be viewed here.. ...
Forward genetics approaches are not popularly applied in non-model plants due to their complex genomes, long life cycles, backward genetic studies etc. Researchers have to adopt reverse genetic methods to characterize gene functions in non-model plants individually, the efficiency of which is usually low. In this study, we report a gain-of-function in Arabidopsis (GAINA) strategy which can be used for batch identification of functional genes in a plant species. This strategy aims to obtain the gain-of-function of rubber tree genes through overexpressing transformation ready full-length cDNA libraries in Arabidopsis. An initial transformation test produced about two thousand independent transgenic Arabidopsis lines, in which multiple obvious aberrant phenotypes were observed, suggesting the gain-of-function of rubber tree genes. The transferred genes were further isolated and identified. One gene identified to be metallothionein-like protein type 3 gene was further transferred into Arabidopsis and
TY - JOUR. T1 - Genetic analysis of osmotic and cold stress signal transduction in Arabidopsis. T2 - Interactions and convergence of abscisic acid-dependent and abscisic acid-independent pathways. AU - Ishitani, Manabu. AU - Xiong, Liming. AU - Stevenson, Becky. AU - Zhu, Jian Kang. PY - 1997/11/1. Y1 - 1997/11/1. N2 - To dissect genetically the complex network of osmotic and cold stress signaling, we constructed lines of Arabidopsis plants displaying bioluminescence in response to low temperature, drought, salinity, and the phytohormone abscisic acid (ABA). This was achieved by introducing into Arabidopsis plants a chimeric gene construct consisting of the firefly luciferase coding sequence (LUC) under the control of the stress-responsive RD29A promoter. LUC activity in the transgenic plants, as assessed by using in vivo luminescence imaging, faithfully reports the expression of the endogenous RD29A gene. A large number of cos (for constitutive expression of osmotically responsive genes), los ...
Read Isolation and Gene Expression Analysis of Arabidopsis thaliana Mutants With Constitutive Expression of ATL2, an Early Elicitor-Response RING-H2 Zinc-Finger Gene, Genetics on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Phytohormones auxins play an important role in plant growth development and abiotic stress responses. The active auxin may be released from the amino acid conjugates by the action of auxin-amidohydrolase enzymes, which thereby participate in the auxin homeostasis. The aim of this study was to investigate the role of auxin-amidohydrolase AtILL2 from Arabidopsis thaliana in the plant response to salinity and osmotic stress. Four homozygous lines of A. thaliana ecotypes Ws of potential overexpressors for AtILL2 fusion protein with GFP and / or His tags were analyzed. The presence of transgene was verified as insertion into DNA, RNA transcript, and recombinant protein by using PCR, RT-PCR, SDS-PAGE, affinity chromatography and western-hybridization assay. In three lines, the presence of transgene in genomic DNA and RNA transcript was confirmed, although the recombinant protein was not demonstrated in any of the lines. Root growth bioassay confirmed the increased resistance of one line to the ...
MADS domain transcription factors play important roles in various developmental processes in flowering plants. Members of this family play a prominent role in the transition to flowering and the specification of floral organ identity. Several studies reported mRNA expression patterns of the genes encoding these MADS domain proteins, however, these studies do not provide the necessary information on the temporal and spatial localisation of the proteins. We have made GREEN FLUORESCENT PROTEIN (GFP) translational fusions with the four MADS domain proteins SEPALLATA3, AGAMOUS, FRUITFULL and APETALA1 from the model plant Arabidopsis thaliana and analysed the protein localisation patterns in living plant tissues by confocal laser scanning microscopy (CLSM). We unravelled the protein localisation patterns of the four MADS domain proteins at a cellular and subcellular level in inflorescence and floral meristems, during development of the early flower bud stages, and during further differentiation of the floral
Guazatine is a potent inhibitor of polyamine oxidase (PAO) activity. In agriculture, guazatine is used as non-systemic contact fungicide efficient in the protection of cereals and citrus fruits against disease. The composition of guazatine is complex, mainly constituted by a mixture of synthetic guanidated polyamines (polyaminoguanidines). Here, we have studied the effects from exposure to guazatine in the weed Arabidopsis thaliana. We report that micromolar concentrations of guazatine are sufficient to inhibit growth of Arabidopsis seedlings and induce chlorosis, whereas germination is barely affected. We observed the occurrence of quantitative variation in the response to guazatine between 107 randomly chosen Arabidopsis accessions. This enabled us to undertake genome-wide association (GWA) mapping that identified a locus on chromosome one associated with guazatine tolerance. CHLOROPHYLLASE 1 (CLH1) within this locus was studied as candidate gene, together with its paralog (CLH2). The analysis ...
It has been more than 50 years since Arabidopsis (Arabidopsis thaliana) was first introduced as a model organism to understand basic processes in plant biology. A well-organized scientific community has used this small reference plant species to make numerous fundamental plant biology discoveries (Provart et al., 2016). Due to an extremely well-annotated genome and advances in high-throughput sequencing, our understanding of this organism and other plant species has become even more intricate and complex. Computational resources, including CyVerse,3 Araport,4 The Arabidopsis Information Resource (TAIR),5 and BAR,6 have further facilitated novel findings with just the click of a mouse. As we move toward understanding biological systems, Arabidopsis researchers will need to use more quantitative and computational approaches to extract novel biological findings from these data. Here, we discuss guidelines, skill sets, and core competencies that should be considered when developing curricula or ...
Various Arabidopsis thaliana (mouse-ear cress) hypothetical proteins. Doerks T, Huber S, Buchner E, Bork P (April 2002). "BSD: ... synapse-associated proteins and DOS2-like proteins in which it is found. It is also found in several hypothetical proteins. The ... Yeast DOS2 protein, involved in single-copy DNA replication and ubiquitination. Drosophila synapse-associated protein SAP47. ... Some proteins known to contain one or two BSD domains are listed below: Mammalian TFIIH basal transcription factor complex p62 ...
Arabidopsis RD22 drought induced protein. Maize ZRP2, a protein of unknown function in cortex parenchyma. Tomato PG1beta, the ... Soybean USP-like proteins ADR6 (or SALI5-4A), an auxin-repressible, aluminium-inducible protein and SALI3-2, a protein that is ... Some proteins known to contain a BURP domain are listed below: Brassica protein BNM2, which is expressed during the induction ... It is found in the C-terminal part of a number of plant cell wall proteins, which are defined not only by the BURP domain, but ...
Volvox major cell adhesion protein (2 FAS1 domains). Arabidopsis thaliana fasciclin-like arabinogalactan proteins (2 FAS1 ... Proteins known to contain a FAS1 domain include: Fasciclin I (4 FAS1 domains). Human TGF-beta induced Ig-H3 (BIgH3) protein (4 ... FAS1 domains are present in many secreted and membrane-anchored proteins. These proteins are usually GPI anchored and consist ... Bacterial immunogenic protein MPT70 (1 FAS1 domain). The FAS1 domains of both human periostin and BIgH3 proteins were found to ...
Zemach A, Grafi G (June 2003). "Characterization of Arabidopsis thaliana methyl-CpG-binding domain (MBD) proteins". Plant J. 34 ... MBD-containing proteins appear to act as structural proteins, which recruit a variety of histone deacetylase (HDAC) complexes ... In human MBD3 and SETDB1, the MBD has been shown to mediate protein-protein interactions. MBDs are also found in DNA ... Effects of DNA methylation are mediated through proteins that bind to symmetrically methylated CpGs. Such proteins contain a ...
"Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis". Plant Physiology. 122 (1): 35-48. doi:10.1104/pp ... Villin has the ability to dimerize and the dimerization site is located at the amino end of the protein. Villin is an actin ... Villin is a 92.5 kDa tissue-specific actin-binding protein associated with the actin core bundle of the brush border. Villin ... The headpiece domain is a commonly studied protein in molecular dynamics due to its small size and fast folding kinetics and ...
Whitley P, Hinz S, Doughty J (December 2009). "Arabidopsis FAB1/PIKfyve proteins are essential for development of viable pollen ... By directly binding membrane PtdIns(3)P, the FYVE finger domain of PIKfyve is essential in localizing the protein to the ... December 2008). "VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and ... The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 6 (1): 63-70. ...
"Expression of chalcone synthase and chalcone isomerase proteins in Arabidopsis seedlings". Plant Mol. Biol. 35 (3): 377-81. doi ... Studies have shown that these enzymes interact via protein-protein interactions. Through FLIM FRET, it was shown that CHS ... Phe215 and Phe265 are two other important amino acids that act as "gatekeepers" to block the lower protein of the opening ... CHS exists as a homodimeric protein with each monomer approximately 42-45 kDa in size. Each monomer possesses a β-keto synthase ...
Gu J (2000). Identification of proteins interacting with lupin and Arabidopsis tRNA nucleotidyltransferase (MSc). Concordia ... Protein synthesis takes place in cytosolic ribosomes, mitochondria (mitoribosomes), and in plants, the plastids (chloroplast ... Complementation studies in yeast using cDNA derived from Arabidopsis thaliana or Lupinus albus genes demonstrate the biological ... ribosomes). Each of these compartments requires a complete set of functional tRNAs to carry out protein synthesis. The ...
Pan, Ruiqin; Lee, Y.-R. Julie; Liu, Bo (2004). "Localization of two homologous Arabidopsis kinesin-related proteins in the ... "Somatic Cytokinesis and Pollen Maturation in Arabidopsis Depend on TPLATE, Which Has Domains Similar to Coat Proteins". The ... "Nuclear-localized subtype of end-binding 1 protein regulates spindle organization in Arabidopsis". J Cell Sci. 123 (3): 451-459 ... "Microtubule-Associated Kinase-like Protein RUNKEL Needed for Cell Plate Expansion in Arabidopsis Cytokinesis". Current Biology ...
... proteins from Arabidopsis thaliana". Plant Physiology and Biochemistry. 139: 293-306. doi:10.1016/j.plaphy.2019.03.027. PMID ... Once DnaJ, a co-chaperone, brings an unfolded protein to DnaK ATP is hydrolyzed to ADP to facilitate folding of the protein. At ... GrpE (Gro-P like protein E) is a bacterial nucleotide exchange factor that is important for regulation of protein folding ... The thermal regulation of DnaK slows protein folding and prevents unfolded proteins from accumulating in the cytoplasm at high ...
"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis". The Plant Journal. 34 (6): 753-67. doi: ... to a lysine residue on the target protein. Once a protein has been tagged with one ubiquitin molecule, additional rounds of ... The E3 molecule is responsible for binding the target protein substrate and transferring the ubiquitin from the E2 cysteine to ... The ubiquitination process covalently attaches ubiquitin, a short protein of 76 amino acids, ...
"MADS-box protein complexes control carpel and ovule development in Arabidopsis". The Plant Cell. 15 (11): 2603-11. doi:10.1105/ ... "Dimerization Specificity of Arabidopsis MADS Domain Homeotic Proteins APETALA1, APETALA3, PISTILLATA, and AGAMOUS". Proceedings ... The genes that are closer homologs of AG in Petunia are pMADS3 and floral-binding protein 6 (FBP6).[28] ... In Arabidopsis and Antirrhinum, the two taxa on which models are based, these mutations always affect adjacent verticils. This ...
Hundertmark, M; Hincha, DK (4 March 2008). "LEA (late embryogenesis abundant) proteins and their encoding genes in Arabidopsis ... There are other proteins in the cell that play a similar role in the recovery of drought treated plants. These proteins are ... The protein has since been referred to as dehydrin and has been the identified as the genetic basis of drought tolerance in ... They are stress proteins with a high number of charged amino acids that belong to the Group II Late Embryogenesis Abundant (LEA ...
March 2005). "High heterogeneity within the ribosomal proteins of the Arabidopsis thaliana 80S ribosome". Plant Mol. Biol. 57 ( ... Universally conserved proteins are shown in blue. These proteins have homologs in eukaryotes, archaea and bacteria. Proteins ... the eukaryote-specific protein segments engage in a multitude of protein-protein interactions. Long distance interactions are ... In eukaryotes, the small subunit protein RPS27A (or eS31) and the large subunit protein RPL40 (or eL40) are processed ...
Bridges, D; Fraser ME; Moorhead GB (2005). "Cyclic nucleotide binding proteins in the Arabidopsis thaliana and Oryza sativa ... By phosphorylating proteins, cyclic AMP-dependent protein kinase alters protein activity. cAMP's role in this process ... This releases cAMP into the cell interior, where it stimulates a protein kinase called cyclic AMP-dependent protein kinase. ... and guanine nucleotide-binding proteins (G proteins). The two most well-studied cyclic nucleotides are cyclic AMP (cAMP) and ...
... a Plant-Specific GW Protein, Defines an Additional RNAi-Dependent Chromatin-Based Pathway in Arabidopsis". Molecular Cell. 48 ( ... Genome-wide computational identification of WG/GW Argonaute-binding proteins in Arabidopsis. Nucleic Acids Res. 2010 Jul;38(13 ... "Genome-wide computational identification of WG/GW Argonaute-binding proteins in Arabidopsis". Nucleic Acids Research. 38 (13): ... Klaus F. X. Mayer in the Institute of Bioinformatics/Munich Information Center for Protein Sequences (MIPS) in Munich. He was ...
... splicing affects 3.3% of Arabidopsis protein coding genes. 11% of intron regions were composed of exitrons and 3.7% of ... When exitrons are spliced out of a sequence, it has resulted in internally deleted proteins and affected protein domains, ... The same sequence within a pre mRNA strand can be considered an intron or exon depending on the desired protein to be produced ... As a result, different final mRNA sequences are generated and a large variety of proteins can be made from one single gene. ...
Zhong R, Ye ZH (2003). "The SAC domain-containing protein gene family in Arabidopsis". Plant Physiol. 132 (2): 544-55. doi: ... 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs ... The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 5 (6): 355-64. doi: ... The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (6): 347-55. doi: ...
"Perception of UV-B by the Arabidopsis UVR8 protein". Science. 332 (6025): 103-6. doi:10.1126/science.1200660. PMID 21454788. ... UVR8 is a β-propeller protein with 7 blade-shaped β-sheets. It shares sequence homology with mammalian proteins involved in ... UV-B resistance 8 (UVR8) also known as ultraviolet-B receptor UVR8 is an UV-B - sensing protein found in plants and possibly ... identified as a crucial mediator of a plant's response to UV-B in Arabidopsis thaliana containing a mutation in this protein. ...
The Arabidopsis KNOLLE protein is a cytokinesis-specific syntaxin. J. Cell Biol. 139, 1485-1493. Geldner, N., Anders, N., ... His laboratory discovered and characterized the protein KNOLLE, which is required for cytokinesis and was the first protein ... Lukowitz, W., Mayer, U., Jürgens, G. (1996). Cytokinesis in the Arabidopsis embryo involves the syntaxin-related KNOLLE gene ... Using the genetically-tractable plant Arabidopsis thaliana he conducted the first forward genetic screen (using mutagenesis ...
Zhong R, Ye ZH (2003). "The SAC domain-containing protein gene family in Arabidopsis". Plant Physiology. 132 (2): 544-55. doi: ... Ectopically expressed Sac3 protein has a very short half-life of only ~18 min due to fast degradation in the proteasome. Co- ... In mice, the protein is essential in early postnatal development. In humans, its I41T point mutation in combination with a null ... The founding protein, containing this evolutionarily-conserved domain, has been the first gene product isolated in a screen for ...
The genome of Arabidopsis thaliana, for instance, contains more than 130 ORFs for these proteins of which more than 100 are ... Sánchez-Fernández, R.; Davies, T.G.E.; Coleman, J.O.D; Rea, P.A. (2001). "The Arabidopsis thaliana ABC protein superfamily: a ... Li, Z.-S.; Szcypka, M.; Thiele, D.J.; Rea, P.A. (1996). "The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S- ... Lu, Y.-P.; Li, Z.-S.; Rea, P.A. (1997). "AtMRP1 gene of Arabidopsis thaliana encodes a glutathione S-conjugate pump: isolation ...
"Structure of an ETHE1-like protein from Arabidopsis thaliana". Acta Crystallographica Section D. 62 (Pt 9): 964-70. doi:10.1107 ... Protein ETHE1, mitochondrial, also known as "ethylmalonic encephalopathy 1 protein" and "per sulfide dioxygenase", is a protein ... Sulfur deoxygenates are proteins that function in sulfur metabolism. The ETHE1 protein is thought to catalyze the following ... This gene encodes a protein that is expressed in the thyroid. The ETHE1 protein is thought to localize primarily to the ...
"ZEITLUPE Encodes a Novel Clock-Associated PAS Protein from Arabidopsis". Cell. 101 (3): 319-329. doi:10.1016/S0092-8674(00) ... It is typically located on the C-Terminus of the BHLH protein. PAS domains containing BHLH proteins form a BHLH-Pas protein, ... PAS A is responsible for the protein-protein interactions with other PAS domain proteins, while PAS B has a more versatile role ... period circadian protein Arnt - aryl hydrocarbon receptor nuclear translocator protein Sim - single-minded protein Since the ...
Kepinski, S.; Leyser, O. (2005). "The Arabidopsis F-box protein TIR1 is an auxin receptor". Nature. 435 (7041): 446-51. Bibcode ... Leyser played a world-leading role in promoting Arabidopsis as a key model organism in modern biology and has provided ... Leyser, Henrietta Miriam Ottoline (1990). An analysis of fasciated mutants of Arabidopsis thaliana and the role of cytokinin in ... the Genomic Arabidopsis Resource Network". Trends in Plant Science. 7 (4): 145-7. doi:10.1016/s1360-1385(01)02224-5. PMID ...
May 2007). "FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis". Science. 316 (5827 ... Jaeger KE, Wigge PA (June 2007). "FT protein acts as a long-range signal in Arabidopsis". Current Biology. 17 (12): 1050-4. doi ... However, the Hd3a protein is found in neither of these places but instead accumulates in the SAM which shows that Hd3a protein ... CO protein promotes transcription of another gene called Flowering Locus T (FT). By this mechanism, CO protein may only reach ...
The activity of karrikins requires an F-box protein named MORE AXILLARY GROWTH-2 (MAX2) in Arabidopsis. This protein is also ... In Arabidopsis, Karrikins work in a similar way to strigolactones; they require homologous proteins known as KARRIKIN- ... May 2011). "F-box protein MAX2 has dual roles in karrikin and strigolactone signaling in Arabidopsis thaliana". Proceedings of ... The gene for KAI2 protein is present in lower plants including algae and mosses, whereas the DWARF14 protein evolved with seed ...
"Structure of the B3 domain from Arabidopsis thaliana protein At1g16640". Protein Sci. 14 (9): 2478-83. doi:10.1110/ps.051606305 ... In Arabidopsis thaliana, there are three main families of transcription factors that contain B3 domain: ARF (Auxin Response ... Database of Arabidopsis Transcription Factors B3, RAV, and ARF family at PlantTFDB:Plant Transcription Factor Database. ... Kagaya Y, Ohmiya K, Hattori T (1999). "RAV1, a novel DNA-binding protein, binds to bipartite recognition sequence through two ...
2007). "The PEROXIN11 Protein Family Controls Peroxisome Proliferation in Arabidopsis". Plant Cell. 19 (1): 333-50. doi:10.1105 ... Peroxisomal membrane protein 11A is a protein that in humans is encoded by the PEX11A gene. GRCh38: Ensembl release 89: ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038 ... 2000). "Pex19 Binds Multiple Peroxisomal Membrane Proteins, Is Predominantly Cytoplasmic, and Is Required for Peroxisome ...
... non-protein-coding genes, and chromosomal structural elements) under selection for biological function.. " Mouse Genome ... Arabidopsis thaliana. 阿拉伯芥(擬南芥) 125,000,000 25,500 Drosophila melanogaster. 黑腹果蠅 180,000,000 13,350 ... This proportion is much higher than can be explained by protein-coding sequences alone, implying that the genome contains many ...
"The Arabidopsis Book. 11: e0163. doi:10.1199/tab.0163. PMC 3711357. PMID 23864837.. ... The concentration of photosynthetic structures in leaves requires that they be richer in protein, minerals, and sugars than, ... further processed by chemical synthesis into more complex organic molecules such as proteins or cellulose, the basic structural ... chemicals which hinder the digestion of proteins and have an unpleasant taste. Animals that are specialized to eat leaves are ...
Arabidopsis thaliana has two homologous proteins, AtToc33 and AtToc34 (The At stands for Arabidopsis thaliana),[38][46] which ... A family of Toc159 proteins, Toc159, Toc132, Toc120, and Toc90 have been found in Arabidopsis thaliana. They vary in the length ... Protein targeting and importEdit. See also: Protein targeting. The movement of so many chloroplast genes to the nucleus means ... A protein kinase drifting around on the outer chloroplast membrane can use ATP to add a phosphate group to the Toc34 protein, ...
Kurstaki Insect Control Protein". Nature Biotechnology. 7 (12): 1265-1269. doi:10.1038/nbt1289-1265.. ... One group added a transcription factor for the production of anthocyanin from Arabidopsis thaliana[33] whereas another used ... "Fruit Cell Wall Proteins Help Fungus Turn Tomatoes From Ripe To Rotten". Science Daily. Jan 31, 2008. Retrieved 29 August 2010. ... This tomato gained the moniker "fish tomato".[16] The antifreeze protein was found to inhibit ice recrystallization in the ...
... annotated collection of all publicly available nucleotide sequences and their protein translations. It is produced and ... Arabidopsis thaliana 1.144226616×10. ^. 9 Canis lupus familiaris 951,238,343 Vitis vinifera 999,010,073 ...
The Arabidopsis Initiative (2000). "Analysis of the genome sequence of the flowering plant Arabidopsis thaliana". Nature 408. ... "A structural perspective on protein-protein interactions" (PDF). Current Opinion in Structural Biology 14. Páxs. 313-324. ... "Protein Engineering 7 (7). ISSN 1741-0134, Páxs. 841-848.. *↑ 70,0 70,1 Thompson, J. D.; et al. (1994). "CLUSTAL W: improving ... 2005). Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins (en inglés) (third edition ed.). Wiley. ISBN 0- ...
2002). "The NADPH:quinone oxidoreductase P1-zeta-crystallin in Arabidopsis catalyzes the alpha,beta-hydrogenation of 2-alkenals ... proteins. In enzymology, a 2-alkenal reductase (EC is an enzyme that catalyzes the chemical reaction ...
A.D. Hershey and Martha Chase, "Independent Functions of Viral Protein and Nucleic Acid in Growth of Bacteriophage," J. General ... where expert staff raise crops and Arabidopsis plants for studies. Seven CSHL faculty members conduct research primarily in ... the adenovirus E1A proteins bind to the retinoblastoma gene product". Nature. 334 (6178): 124-9. Bibcode:1988Natur.334..124W. ... a highly conserved protein complex that recognizes and binds to specific DNA sequences, marking starting points for replication ...
FT protein movement contributes to long distance signalling in floral induction of Arabidopsis. Science. 2007, 316 (5827): 1030 ... Movement Protein of Tobacco Mosaic Virus Modifies Plasmodesmatal Size Exclusion Limit Science 20 October 1989: Vol. 246. no. ... Not just another hole in the wall: understanding intercellular protein trafficking. Genes Dev. January 2005, 19 (2): 189-95. ... JE Radford and RG White (1998) Localization of a myosin‐like protein to plasmodesmata. Plant Journal 14: 743-750 ...
FITC reacts with the amine groups of many biologically relevant compounds including intracellular proteins to form a thiourea ... "Fluorescein Transport Assay to Assess Bulk Flow of Molecules Through the Hypocotyl in Arabidopsis thaliana". Bio-Protocol. 8 ... allowing biologists to target the fluorophore to specific proteins or structures within cells. This application is common in ...
Altered response to Gravity is a Peripheral Membrane Protein That Modulates Gravity-Induced Cytoplasmic Alkalinization and ... Statolith Sedimentation Kinetics and Force Transduction to the Cortical Endoplasmic Reticulum in Gravity-Sensing Arabidopsis ...
"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis". The Plant Journal. 34 (6): 753-67. doi: ... The protein degradation processEdit. Ribbon diagram of ubiquitin, the highly conserved protein that serves as a molecular tag ... Proteasomes are protein complexes which degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks ... Proteins are tagged for degradation with a small protein called ubiquitin. The tagging reaction is catalyzed by enzymes called ...
This leads to antiviral protein production, such as protein kinase R, which inhibits viral protein synthesis, or the 2′,5′- ... The first plant receptors of conserved microbial signatures were identified in rice (XA21, 1995)[34][35] and in Arabidopsis ( ... "Resistance" (R) proteins, encoded by R genes, are widely present in plants and detect pathogens. These proteins contain domains ... The cascade is composed of many plasma proteins, synthesized in the liver, primarily by hepatocytes. The proteins work together ...
Savini I, Rossi A, Pierro C, Avigliano L, Catani MV (April 2008). "SVCT1 and SVCT2: key proteins for vitamin C uptake". Amino ... Leferink, N. G.; van den Berg, W. A.; van Berkel, W. J. (2008). "L-Galactono-γ-lactone Dehydrogenase from Arabidopsis thaliana ... The HIFalpha prolyl hydroxylases, termed PHDs/EGLNs (prolyl hydroxylase domain proteins/EGL nine homologues), bind to a ... which lose SVCT proteins during maturation.[105] In both vitamin C synthesizers (example: rat) and non-synthesizers (example: ...
... anchored proteins: GPI-anchored proteins in liposomes and cells show similar behavior". Proceedings of the National Academy of ... Role of phospholipase D alpha in freezing-induced lipid changes in Arabidopsis". The Journal of Biological Chemistry. 277 (35 ... The roles of lysoPA, PA, and DAG in promoting membrane curvature do not preclude a role in recruiting proteins to the membrane ... PA acts as a signaling lipid, recruiting cytosolic proteins to appropriate membranes (e.g., sphingosine kinase 1[8]). ...
Opsins are proteins and the retinal-binding visual pigments found in the photoreceptor cells in the retinas of eyes. An opsin ... Schmidt, Holger; Kurtzer, Robert; Eisenreich, Wolfgang; Schwab, Wilfried (2006). "The Carotenase AtCCD1 from Arabidopsis ... An opsin protein surrounds a molecule of retinal, awaiting detection of a photon. Once retinal captures a photon, retinal's ... Opsins are prototypical G protein-coupled receptors (GPCRs).[13] Bovine rhodopsin, the opsin of the rod cells of cattle, was ...
Arabidopsis thaliana has two homologous proteins, AtToc33 and AtToc34 (The At stands for Arabidopsis thaliana),[42][50] which ... A family of Toc159 proteins, Toc159, Toc132, Toc120, and Toc90 have been found in Arabidopsis thaliana. They vary in the length ... Protein targeting and import[edit]. See also: Protein targeting. The movement of so many chloroplast genes to the nucleus means ... Protein synthesis[edit]. See also: Transcription and translation. Protein synthesis within chloroplasts relies on an RNA ...
Many flowering plants (angiosperms) use a photoreceptor protein, such as phytochrome or cryptochrome,[1] to sense seasonal ... showed that manipulations of the red-to far-red ratio in Arabidopsis can alter flowering. They discovered that plants tend to ... "Regulation of photoperiodic flowering by Arabidopsis photoreceptors". Proceedings of the National Academy of Sciences of the ...
Simpson, Gordon G.; Dean, Caroline (2002-04-12). "Arabidopsis, the Rosetta Stone of Flowering Time?". Science. 296 (5566): 285- ... Similarly, [email protected] is a distributed computing project for predicting protein structures from amino acid sequences (or ... The flowering plant Arabidopsis thaliana has been called the "Rosetta Stone of flowering time".[90] A Gamma ray burst (GRB) ...
... green fluorescent protein with the protein of interest).. Techniques used for horizontal scanning[edit]. Four types of confocal ... in root meristem cells 4-day old Arabidopsis thaliana (Col-0). Scale bar: 5 um. ... GFP fusion protein being expressed in Nicotiana benthamiana. The fluorescence is visible by confocal microscopy. ...
To interact with host plant proteins many Agrobacterium virulence proteins encoded by vir genes. Agrobacterium vir genes ... Hence, this method is used widely to study gene function in plants, such as the model plant Arabidopsis thaliana. While ... VirD2 is the protein that caps the 5′ end of the transferred T-strand by covalent attachment and is transported to the host ... It is then directed into the nucleus via interactions with the host cell proteins such as importin a, bacterial VirE3, and ...
... binding protein. eIF-4E and eIF-4G block the decapping enzyme (DCP2), and poly(A)-binding protein blocks the exosome complex, ... "The Arabidopsis CBP20 targets the cap-binding complex to the nucleus, and is stabilized by CBP80". The Plant Journal. 59 (5): ... Proteins that are needed in a particular region of the cell can also be translated there; in such a case, the 3' UTR may ... Proteins that bind to either the 3' or 5' UTR may affect translation by influencing the ribosome's ability to bind to the mRNA ...
That data, along with data on the genetic modification itself and resulting proteins (or lack of protein), is submitted to ... Used to produce proteins that humans lack. This can be for therapeutic use, for example, treatment of diseases in other mammals ... Study of the introduced DNA and the new proteins or metabolites that it produces; ... or of proteins present in a food or crop. The goal would be to compare overall the biochemical profile of a new food to an ...
The model is based upon studies of mutants in Arabidopsis thaliana and snapdragon, Antirrhinum majus. For example, when there ... Whereas the pollen of animal-pollinated flowers tends to be large-grained, sticky, and rich in protein (another "reward" for ... Whereas the pollen of entomophilous flowers tends to be large-grained, sticky, and rich in protein (another "reward" for ... FLC confers a flowering response to vernalization by repressing meristem competence and systemic signaling in Arabidopsis". ...
This is due to the tendency of tannins to react with proteins, such as the ones found in saliva.[14] In food and wine pairing, ... "Flavonoids act as negative regulators of auxin transport in vivo in arabidopsis". Plant Physiol. 126 (2): 524-35. doi:10.1104/ ... proteins and lipids from oxidative damage pursuant to Article 13(1) of Regulation (EC) No 1924/20061". EFSA Journal. 8 (2): ... "Interactions of Grape Seed Tannins with Salivary Proteins". Journal of Agricultural and Food Chemistry. 47 (1): 42-7. doi: ...
Graciet E., Lebreton S., Gontero B. Emergence of new regulatory mechanisms in the Benson-Calvin pathway via protein-protein ... Light modulation of Rubisco in Arabidopsis requires a capacity for redox regulation of the larger Rubisco activase isoform.. „ ... Simova-Stoilova L., Stoyanova Z. , Demirevska-Kepova K.. ONTOGENIC CHANGES IN LEAF PIGMENTS, TOTAL SOLUBLE PROTEIN AND RUBISCO ... A. R., Ogren W.L.. A soluble chloroplast protein catalyzes ribulosebisphosphate carboxylase/oxygenase activation in vivo. „ ...
Ethylene is perceived by a family of five transmembrane protein dimers such as the ETR1 protein in Arabidopsis. The genes ... The effects of salinity have been studied on Arabidopsis plants that have mutated ERS1 and EIN4 proteins.[36] These proteins ... "EIN2 regulates salt stress response and interacts with a MA3 domain-containing protein ECIP1 in Arabidopsis". Plant, Cell & ... Stimulation of Arabidopsis hypocotyl elongation [40]. *In pollination, when the pollen reaches the stigma, the precursor of the ...
Secondary databases: UniProt, database of protein sequences grouping together Swiss-Prot, TrEMBL and Protein Information ... Specialised genomic databases: BOLD, Saccharomyces Genome Database, FlyBase, VectorBase, WormBase, PHI-base, Arabidopsis ... Other databases: Protein Data Bank, Ensembl and InterPro. * ... a number of model organism databases and multi-species protein ...
The mechanism involves acetylation and/or methylation of specific amino acids in histone H3, a protein closely associated with ... that hybrid vigor in an allopolyploid hybrid of two Arabidopsis species was due to epigenetic control in the upstream regions ... and means that more antigenic proteins on any pathogen are likely to be recognised, giving a greater range of T-cell activation ... parental genome dosage and hybridity contributions to heterosis in Arabidopsis thaliana". The New Phytologist. 209 (2): 590-599 ...
Expression profiles in Arabidopsis thaliana (Protein) [Bio-Analytic Resource] Expression profiles in Arabidopsis thaliana ( ... Uncovering Arabidopsis membrane protein interactome enriched in transporters using mating-based split ubiquitin assays and ... Uncovering Arabidopsis membrane protein interactome enriched in transporters using mating-based split ubiquitin assays and ... Subcellular localisation in Arabidopsis thaliana [Bio-Analytic Resource] Subcellular localisation in Arabidopsis thaliana. Bio- ...
unnamed protein product [Arabidopsis thaliana] unnamed protein product [Arabidopsis thaliana]. gi,8809640,dbj,BAA97191.1, ... Transcript/Protein Information [PANTHER Classification System] Transcript/Protein Information. PANTHER Classification System ... RefSeq protein isoforms See 2 reference sequence protein isoforms for the GCD1 gene. ... Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.. Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, ...
On the basis of these results, we propose that GA distribution and activity in Arabidopsis is partly regulated by NPF3 acting ... Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in GA transport. We show that ... Phylogenetic tree of the Arabidopsis NRT/PTR family. Protein sequences for Arabidopsis thaliana NRT/PTR family members were ... Arabidopsis NPF1.1 and NPF4.6/AIT1 transport auxin and ABA, respectively12,30. Recent work showed that various NPF proteins can ...
I am trying to isolate plastid proteins instead of total proteins from ,Arabidopsis but I do not have any clues. My thought is ... Arabidopsis] Isolation of plastid protein. Jerry D. Cohen via (by cohen047 At Fri Jan 19 15:39 ... The protocol has not been tested for protein import activity but is optimized for chloroplast proteome yield and purity and ... to identify the ,subcellular location of the expressed proteins. If you have any idea or ,protocols, please send it to me. , ...
Luminidependens (LD) is an Arabidopsis protein with prion behavior Message Subject (Your Name) has sent you a message from PNAS ... Luminidependens (LD) is an Arabidopsis protein with prion behavior. Sohini Chakrabortee, Can Kayatekin, Greg A. Newby, Marc L. ... Applying methods we used to uncover yeast prions, we identified nearly 500 Arabidopsis proteins that harbor potential prion- ... Applying methods we used to uncover yeast prions, we identified nearly 500 Arabidopsis proteins that harbor potential prion- ...
Each Arabidopsis protein was searched for the presence of PrDs by using a HMM that parsed each protein into prion-like and non- ... S1A). All three chimeric proteins formed distinct foci, a common characteristic of prion proteins (Fig. 1B). For each protein, ... 500 Arabidopsis proteins that have distinct prion-like domains (PrDs). These proteins showed a functional enrichment for ... we showed that three domains from Arabidopsis proteins (LD, FPA, and FCA) had some prion-like properties. All of these proteins ...
... xinyou gao via (by gaoxinyou3 from Tue ... Please help! I have some T2 seeds and seedlings of Arabidopsis transformed with GFP-tagged protein May I use T2 seeds and ... Previous message: [Arabidopsis] plant metabolic pathway database curator position available *Next message: [Arabidopsis] urban ... Previous message: [Arabidopsis] plant metabolic pathway database curator position available *Next message: [Arabidopsis] urban ...
The Athsp101 complementary DNA (cDNA) corresponds to the gene identification At1g74310 in the Arabidopsis genome sequence. An … ... was also among the first plant species from which Hsp100 proteins were characterized. ... Arabidopsis thaliana, the first plant for which the entire genome sequence is available, ... Arabidopsis thaliana Hsp100 proteins: kith and kin Cell Stress Chaperones. 2001 Jul;6(3):219-24. doi: 10.1379/1466-1268(2001) ...
Protein. Similar proteins. Organisms. Length. Cluster ID. Cluster name. Size. P92940. Q9LEX1. A0A178VK33. UPI000BBEAECF. R0H773 ... Protein. Similar proteins. Organisms. Length. Cluster ID. Cluster name. Size. P92940. Q9LEX1. A0A178VK33. UPI000BBEAECF. R0H773 ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... Integrated resource of protein families, domains and functional sites. More...InterProi. View protein in InterPro. IPR000008. ...
Identification of safener binding proteins in Arabidopsis using chemical probes. A research project of the Centre for Bioactive ...
Arabidopsis] Gateway-compatible pOp/LhGR fluorescent protein tagging. Mathew G. Lewsey via (by mlewsey ... Previous message: [Arabidopsis] ICAR 2015- Paris- Website is Open *Next message: [Arabidopsis] Research Professor Position at U ... Previous message: [Arabidopsis] ICAR 2015- Paris- Website is Open *Next message: [Arabidopsis] Research Professor Position at U ...
Arabidopsis peroxisomal matrix protein degradation is influenced by proteins implicated in matrix protein import, receptor ... A) Likely functions of Arabidopsis peroxins (numbered ovals) in peroxisome matrix protein import based on data from Arabidopsis ... Peroxisomes import matrix proteins from the cytosol with the assistance of peroxin (PEX) proteins. Most matrix proteins are ... 2009 Arabidopsis LON2 is necessary for peroxisomal function and sustained matrix protein import. Plant Physiol. 151: 1354-1365. ...
Protein-L-isoaspartate (D-aspartate) O-methyltransferases (EC that catalyze the transfer of methyl groups from S- ... adenosylmethionine to abnormal L-isoaspartyl and D-aspartyl residues in a variety of peptides and proteins are widely ... A distinctly regulated protein repair L-isoaspartylmethyltransferase from Arabidopsis thaliana Plant Mol Biol. 1996 Feb;30(4): ... Protein-L-isoaspartate (D-aspartate) O-methyltransferases (EC that catalyze the transfer of methyl groups from S- ...
... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... Integrated resource of protein families, domains and functional sites. More...InterProi. View protein in InterPro. IPR035513 ... p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.,p>,a ... p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different ...
Moreover, these vesicles can harbor distinct proteins and do not form normally when fatty acid β-oxidation, a core function of ... Here the authors show that Arabidopsis peroxisomes contain extensive internal vesicles that form from the bounding membrane in ... Although peroxisomes typically are considered to consist of a single membrane enclosing a protein lumen, more complex ... Here we exploit the unusually large size of Arabidopsis peroxisomes to demonstrate that peroxisomes have extensive internal ...
The Ubiquitin-Related Protein RUB1 and Auxin Response in Arabidopsis. By J. C. del Pozo, C. Timpte, S. Tan, J. Callis, M. ... The Ubiquitin-Related Protein RUB1 and Auxin Response in Arabidopsis. By J. C. del Pozo, C. Timpte, S. Tan, J. Callis, M. ... The Ubiquitin-Related Protein RUB1 and Auxin Response in Arabidopsis Message Subject. (Your Name) has forwarded a page to you ...
In Arabidopsis, the receptor kinase CLAVATA1 (CLV1) binds to the small secreted polypeptide CLV3, and three proteins act as key ... Receptor-like protein kinase genes of Arabidopsis thaliana. Plant J. 3: 451-456.Google Scholar ... The ArabidopsisCLV3-like (CLE) genes are expressed in diverse tissues and encode secreted proteins. ... The Arabidopsis ERECTA gene encodes a putative receptor protein kinase with extracellular leucine-rich repeats. Plant Cell 8: ...
DCL1 protein, Arabidopsis. Known as: suspensor1 protein, Arabidopsis, CAF protein, Arabidopsis, SUS1 protein, Arabidopsis ... CARPEL FACTORY, a Dicer Homolog, and HEN1, a Novel Protein, Act in microRNA Metabolism in Arabidopsis thaliana ... To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we ... Arabidopsis thaliana floral meristems are determinate structures that produce a defined number of organs, after which cell… ...
The Arabidopsis nhl3 gene encodes a plasma membrane protein and its overexpression correlates with increased resistance to ... Genomics and localization of the Arabidopsis DHHC-CRD S-acyltransferase protein family. Plant Physiology 160: 1597-1612.. * ... Heterotrimeric G protein gamma subunits provide functional selectivity in gbetagamma dimer signaling in Arabidopsis. Plant Cell ... Expansion of the receptor-like kinase/pelle gene family and receptor-like proteins in Arabidopsis. Plant Physiology 132: 530- ...
We found that members of the plant-specific SC35-Like (SCL) Arabidopsis SR protein subfamily are distinctively responsive to ... Using real-time RT-qPCR, we analyzed total steady-state transcript levels of the 18 SR and two SR-like genes from Arabidopsis ... Finally, despite pervasiveness of established ABA-responsive promoter elements in Arabidopsis SR and SR-like genes, their ... proteins are major modulators of alternative splicing, a key generator of proteomic diversity and flexible means of regulating ...
The results reveal that these proteins, rather than being rapidly induced stress proteins, are primarily constitutive proteins ... ClpP1 and ClpP6 proteins. Potential differential regulation among these Clp proteins was analysed at both the mRNA and protein ... Characterisation of the Clp Proteins in Arabidopsis thaliana. Zheng, Bo Umeå University, Faculty of Science and Technology, ... Open this publication in new window or tab ,,Characterization of Chloroplast Clp proteins in Arabidopsis: Localization, tissue ...
Scientific Experts about Experts and Doctors on arabidopsis proteins in Germany ... proto oncogene proteins c myb*arabidopsis agamous protein*cotyledon*gibberellins*light harvesting protein complexes*botrytis* ... Shahriari M, Richter K, Keshavaiah C, Sabovljevic A, Huelskamp M, Schellmann S. The Arabidopsis ESCRT protein-protein ... The receptor-like protein ReMAX of Arabidopsis detects the microbe-associated molecular pattern eMax from Xanthomonas. Plant ...
The proteins of the ELIP family were proposed to function in photoprotection or assembly of thylakoid pigment-protein complexes ... The proteins of the ELIP family were proposed to function in photoprotection or assembly of thylakoid pigment-protein complexes ... Two small One-Helix Proteins (OHPs) are expressed constitutively in green plant tissues and their levels increase in response ... Two small One-Helix Proteins (OHPs) are expressed constitutively in green plant tissues and their levels increase in response ...
A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana ... A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana ... A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana ... A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana ...
PM intrinsic proteins (PIPs)) were already well-known PM proteins. Other proteins, already described as PM resident proteins ... The GRAVY of the Arabidopsis plasma membrane proteins analyzed ranges from −1.6 to +1 (Fig. 3B). Only 20% of the proteins ... Extraction and Analysis of Hydrophobic Proteins from Arabidopsis PM. Protein Extraction-. PM fractions were isolated from ... Low-abundance proteins, including regulatory proteins and rare membrane proteins, are out of the scope of standard proteomic ...
Arabidopsis AtHAL3 protein: a salt tolerance (halotolerance) flavoprotein from plants, shows homology to yeast HAL3; GenBank ... Arabidopsis; ATHAL3b protein, Arabidopsis; HAL3 protein, Arabidopsis; HAL3a protein, Arabidopsis; HAL3b protein, Arabidopsis; ... Amino Acids, Peptides, and Proteins*Proteins: 90489*Plant Proteins: 56*Arabidopsis Proteins: 2*Arabidopsis AtHAL3 protein ... Arabidopsis AtHAL3 protein. Subscribe to New Research on Arabidopsis AtHAL3 protein a salt tolerance (halotolerance) ...
... a class I knotted-like protein; from Arabidopsis; amino acid sequence given in first source; GenBank U32344 ... Arabidopsis STM protein. Subscribe to New Research on Arabidopsis STM protein a class I knotted-like protein; from Arabidopsis ... Amino Acids, Peptides, and Proteins*Proteins: 90489*DNA-Binding Proteins: 1111*Homeodomain Proteins: 55*Arabidopsis STM protein ... STM protein, Arabidopsis; shootmeristemless protein, Arabidopsis. Networked: 0 relevant articles (0 outcomes, 0 trials/studies ...
Arabidopsis scaffold protein RACK1A modulates rare sugar D-allose regulated gibberellin signaling ... "Time-dependent, glucose-regulated Arabidopsis Regulator of G-protein Signaling 1 network". Country unknown/Code not available. ... title = {Time-dependent, glucose-regulated Arabidopsis Regulator of G-protein Signaling 1 network},. author = {Jaiswal, Dinesh ... Published Article: Time-dependent, glucose-regulated Arabidopsis Regulator of G-protein Signaling 1 network ...
... the CCS52A proteins seem likely to be the most similar in function to CDH1/FZR proteins (Fülop et al. 2005). Arabidopsis has ... Among the proteins targeted by the APC/C when activated by these proteins are the mitotic B-type cyclins. Arabidopsis has three ... SIAMESE Cooperates With the CDH1-like Protein CCS52A1 to Establish Endoreplication in Arabidopsis thaliana Trichomes. Remmy ... SIAMESE Cooperates With the CDH1-like Protein CCS52A1 to Establish Endoreplication in Arabidopsis thaliana Trichomes. Remmy ...
Regulation of storage protein gene expression in Arabidopsis. Thomas Kroj, Gil Savino, Christiane Valon, Jérôme Giraudat, ... Regulation of storage protein gene expression in Arabidopsis. Thomas Kroj, Gil Savino, Christiane Valon, Jérôme Giraudat, ... Regulation of storage protein gene expression in Arabidopsis. Thomas Kroj, Gil Savino, Christiane Valon, Jérôme Giraudat, ... Regulation of storage protein gene expression in Arabidopsis Message Subject (Your Name) has sent you a message from ...
  • Encodes a calcium-binding protein annexin (AnnAt6). (
  • We found three pfl mutants that were defective in PEROXIN14 ( PEX14/At5g62810 ), which encodes a peroxisomal membrane protein that assists in importing proteins into the peroxisome matrix, indicating that proteins must enter the peroxisome for efficient degradation. (
  • Finally, one pfl mutant that displayed normal matrix protein import carried a novel lesion in PEROXIN6 ( PEX6/At1g03000 ), which encodes a peroxisome-tethered ATPase that is involved in recycling matrix protein receptors back to the cytosol. (
  • The CLAVATA1 gene encodes a putative receptor kinase that controls shoot and floral meristem size in Arabidopsis . (
  • In Paper II, an Arabidopsis thaliana cDNA was isolated that encodes a homologue of bacterial ClpX. (
  • The ABI1 gene encodes a protein with high similarity to protein serine or threonine phosphatases of type 2C with the novel feature of a putative Ca2+ binding site. (
  • The SIAMESE ( SIM ) gene of Arabidopsis thaliana encodes the founding member of a novel class of plant-specific cyclin-dependent kinase (CDK) inhibitors and is a key regulator of endoreplication during the development of trichomes (shoot epidermal hairs). (
  • CCS52A1 encodes a CDH1/FZR-like protein, a class of proteins that function as activators of the anaphase-promoting complex. (
  • The Arabidopsis genome encodes 1,085 typical protein kinases (M. Gribskov, unpublished data), which is about 4% of the predicted 25,500 genes ( Arabidopsis Genome Initiative, 2000 ). (
  • SAD2/EMA1 encodes an Importin ß protein. (
  • Positional cloning and GFP fusions showed that QUA2, consistent with a role in HG synthesis, encodes a Golgi-localized protein. (
  • Mach J, Castillo A, Hoogstraten R, Greenberg J. The Arabidopsis-accelerated cell death gene ACD2 encodes red chlorophyll catabolite reductase and suppresses the spread of disease symptoms. (
  • The TANG1 gene was isolated by a map-based cloning approach, and encodes a previously uncharacterized novel protein with a predicted Symplekin tight junction protein C-terminal (Symplekin_C). Expression analysis indicates that TANG1 is ubiquitously expressed at moderate levels in different organs and throughout the Arabidopsis life cycle, however, its expression is not affected by high sugar treatment. (
  • The deduced amino acid sequences suggest that the former encodes a secreted protein, and the latter encodes a type 1 membrane protein. (
  • latter encodes a type 1 membrane protein. (
  • The article offers information on the study conducted by the authors related to the ACR11 encodes a novel type of chloroplastic ACT domain repeat protein that is coordinately expressed with GLN2 in Arabidopsis. (
  • it encodes an immunophilin-like protein similar to the p59 FK506-binding protein (FKBP52). (
  • The Arabidopsis genome encodes two groups of dynamin-related proteins (DRPs) that show homology to mammalian dynamin-a protein required for fission of endocytic vesicles during CME. (
  • We have identified an additional member of the CLE gene family in Arabidopsis , which is more similar in gene structure to CLV3 than the other CLE genes. (
  • We show that 24 of the 25 Arabidopsis CLE genes are transcribed in one or more tissues during development, indicating that they do encode functional products. (
  • Two linked pairs of Arabidopsis TNL resistance genes independently confer recognition of bacterial effector AvrRps4. (
  • Using real-time RT-qPCR, we analyzed total steady-state transcript levels of the 18 SR and two SR-like genes from Arabidopsis thaliana in seedlings treated with ABA and in genetic backgrounds with altered expression of the ABA-biosynthesis ABA2 and the ABA-signaling ABI1 and ABI4 genes. (
  • We found that members of the plant-specific SC35-Like (SCL) Arabidopsis SR protein subfamily are distinctively responsive to exogenous ABA, while the expression of seven SR and SR-related genes is affected by alterations in key components of the ABA pathway. (
  • Finally, despite pervasiveness of established ABA-responsive promoter elements in Arabidopsis SR and SR-like genes, their expression is likely governed by additional, yet unidentified cis -acting elements. (
  • Wang J, Schwab R, Czech B, Mica E, Weigel D. Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana. (
  • Chang L, Ramireddy E, Schmülling T. Lateral root formation and growth of Arabidopsis is redundantly regulated by cytokinin metabolism and signalling genes. (
  • AtNEAP proteins are encoded by a small gene familycomposed of three genes and are targeted through a nuclear localisation signal to the nucleuswhere they are anchored at the INM through their C-terminal transmembrane domain.AtNEAPs also possess several long coiled-coil domains reminiscent of the lamin structure inanimals. (
  • Later, in addition to CesA genes, Arabidopsis genome sequencing revealed a large gene family with homology to CesA [ 6 ] named cellulose synthase-like ( Csl ). (
  • RNAi knockdown of genes encoding several of the identified proteins showed that secondary wall formation depends on the coordinated presence of microtubule interacting proteins with nonoverlapping functions: cell wall thickness, cell wall homogeneity, and the pattern and cortical location of the wall are dependent on different proteins. (
  • The Arabidopsis thaliana genome contains genes encoding 10 MAP kinase kinase (MKK) and 20 Map kinase (MAPK) proteins, however the exact relationship between upstream MKK activators and downstream MAPK protein partners has not fully elucidated. (
  • Genetic analyses have identified Arabidopsis ( Arabidopsis thaliana ) genes that play essential roles during embryogenesis. (
  • A distinct class of genes identified by the leafy cotyledon ( lec ) mutations of Arabidopsis play seed-specific roles in both the morphogenesis and maturation phases of embryogenesis (for review, see Harada, 2001 ). (
  • The lack of STN8 caused 50-60% reduction in D1 and D2 phosphorylation, but did not change the phosphorylation level of two peptides that could correspond to light-harvesting proteins encoded by seven different genes in Arabidopsis . (
  • In contrast with the single SEIPIN genes in humans and yeast, there are three SEIPIN homologs in Arabidopsis thaliana , designated SEIPIN1 , SEIPIN2 , and SEIPIN3 . (
  • A component of the translocon in the outer envelope membrane, Toc34, is encoded in Arabidopsis by two homologous genes, atTOC33 and atTOC34. (
  • These results offer a roadmap for further functional characterizations of the protein factors, and for building models when testing the genetic contributions of these genes in plant growth and development. (
  • We have identified a number of genes of the flowering plant Arabidopsis thaliana that are abundantly expressed during embryogenesis. (
  • In this paper we discuss four of these genes, which comprise a gene family: complete genomic nucleotide sequence of two of the genes and partial sequence of the other two shows that they are all homologous to the 12S globulin seed storage protein genes of other angiosperms. (
  • One subfamily contains two genes in the Landsberg erecta strain, but only a single gene in the Columbia strain of Arabidopsis. (
  • We observed no significant difference in the expression profiles of these four genes in ABA-deficient and ABA-insensitive mutants of Arabidopsis, except that the onset of detectable expression of all of the transcripts is slightly delayed in both types of mutants. (
  • Furthermore, the EB1 genes are expressed in various cell types of Arabidopsis , but the expression of EB1c is particularly strong in the meristematic cells where it is targeted to the nucleus by a nuclear localization signal in the C-terminal tail. (
  • Systematic cloning of the genes encoding these proteins is therefore of general interest. (
  • 1997). In this study, we carried out the latter yeast-based signal sequence trap to clone genes from Arabidopsis thaliana encoding secreted and plasma membrane proteins. (
  • Of 144 sequenced cDNA clones, 18% are identical to previously cloned Arabidopsis thaliana genes, 12% are homologous to genes identified from various organisms, and 46% are novel. (
  • All of the isolated genes identical or homologous to previously reported genes are either secreted or plasma membrane proteins, and the remaining novel genes appear to contain functional signal sequences based on computer-aided sequence analysis. (
  • These results indicate that the signal sequence trap method is effective and useful for the isolation of plant genes encoding secreted and plasma membrane proteins. (
  • and useful for the isolati on of plant genes encoding secreted and plasma membrane proteins. (
  • Considering previous findings that (a) AtPQL4 and AtPQL6 are localised in the ER (b) ATPQL mutants show differential expression of genes involved in the unfolded protein response (UPR) and (c) over-expression of AtPQL4 and AtPQL6 impacts on the targeting of other proteins, the observed phenotype could be linked to the unfolded protein response and autophagy that occurs during sugar starvation. (
  • Arabidopsis thaliana UV-Resistance Locus 8 (UVR8) is a UV-B-specific protein that regulates genes concerned with protection against ultraviolet-B (UV-B) radiation. (
  • Some of these genes encode chloroplast proteins including the RNA polymerase sigma factor, sigma 5 (SIG5) and one of the Early Light Induced Proteins in Arabidopsis thaliana (ELIP1). (
  • According to this discovery, UVR8 had been proposed to be involved in regulating chloroplast related genes that encode Photosystem II Reaction Center core proteins, the D1 and D2 proteins. (
  • The present project pursues the identification of key genes with roles in protein O-glycosylation that are crucial for pollen tube growth. (
  • In this project I proposed to use the yeast two hybrid system to isolate genes that encode proteins which interact with the cytoplasmic domain of PERKs. (
  • The Arabidopsis thaliana genome contains two genes with homology to the mitochondrial protein LETM1 (leucine zipper-EF-hand-containing transmembrane protein). (
  • These results support our hypothesis that the two atypical resistance R genes, RPW8.1 and RPW8.2 evolved from duplication and functional diversification (enhancement) of a more ancient component of basal immunity in Arabidopsis (Chapter 2). (
  • Dear all, I am new in using GFP-protein localization. (
  • Although AtHsp101 is cytoplasmic, 5 of the 7 related proteins have predicted plastidial localization signals. (
  • We have also determined the sub-cellular localization of several CLE proteins, and find that they are exported to the plasma membrane or extracellular space. (
  • The plasma membrane localization was confirmed for several proteins, therefore validating such proteomic strategy. (
  • The experiments to reveal the sub-cellular localization and dimerization of the AtSERK1 protein were performed using transient transfections in cowpea mesophyll protoplasts. (
  • The extracellular LRRs, and in particular the N-linked oligosaccharides that are present on them are essential for correct localization of the AtSERK1 protein. (
  • In this study, western blot assay and subcellular localization showed that BcMF18 encoded an approximately 19.5 kDa secretory AGP protein which anchored to extracellular matrices. (
  • Here we report a study on the cellular localization and the enzyme activity of an Arabidopsis CslA family member, AtCslA2. (
  • In this study we constructed a fluorescent fusion of an Arabidopsis CslA family member, AtCslA2-GFP, to identify its final localization in a heterologous system represented by tobacco leaf protoplasts. (
  • The polarity protein BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL) is phosphorylated by MPK3/6 for localization and function in driving divisional asymmetries. (
  • Manipulation of mitogen-activated protein kinase kinase signaling in the Arabidopsis stomatal lineage reveals motifs that contribute to protein localization and signaling specificity. (
  • Using cyan and yellow fluorescent protein fusion constructs, we show that overexpressed Gγ 1 localizes to protoplast membranes, but Gβ exhibits membrane localization only when the Gγ 1 protein is co-overexpressed. (
  • In the present study, we examined the biochemical properties, subcellular localization and tissue-specific distribution of Arabidopsis CML43. (
  • It is possible that the BURP domain represents a general motif for localization of proteins within the cell wall matrix. (
  • I have some T2 seeds and seedlings of Arabidopsis transformed with GFP-tagged protein May I use T2 seeds and seedlings for Confocal laser scanning microscopy? (
  • Arabidopsis peroxisomes are relatively large (~1-2 µm) and can be even larger in young seedlings 9 . (
  • The Arabidopsis thaliana Somatic Embryogenesis Receptor Kinase 1 (AtSERK1) gene is the most closely related Arabidopsis SERK gene and is expressed in developing ovules, early embryos and in the vascular tissues of seedlings and adult plants. (
  • Adequate phenylalanine synthesis mediated by G protein is critical for protection from UV radiation damage in young etiolated Arabidopsis thaliana seedlings. (
  • The present study characterizes an inhibitor of endomembrane protein trafficking, TE1 (trafficking and endocytosis inhibitor 1/TENin1) that reduces gravitropic root bending in Arabidopsis thaliana seedlings. (
  • Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors. (
  • Chapter 4 was designed to further investigate protein-protein interactions between At1g74130 and known substrates. (
  • We show that the Arabidopsis ESCRT interactome possesses a number of protein-protein interactions that are either conserved in yeasts and mammals or distinct to plants. (
  • In this study, the yeast two -hybrid system was used to identify protein-protein interactions between all Arabidopsis MAPKs and MKKs. (
  • Post-translational modifications of proteins, like phosphorylation, often serve as "on-and-off" switches in regulation of cellular activity and may affect protein-protein interactions. (
  • Here, we report two new players, Hypersensitive to Red and Blue 1 (HRB1) and Protein Phosphatase 7 (PP7), and their genetic and biochemical interactions in the control of stomatal aperture. (
  • The protein-protein interactions (PPI) within this pathway are quite well described, but little is known about how the MCS interacts with other pathways in A. thaliana at the level of PPI. (
  • Out of 320 pair-wise protein-protein interaction assays done using the yeast two-hybrid system, 56 (~17%) showed positive interactions. (
  • These interactions organize into three distinct hubs involving the Arabidopsis polyadenylation factors. (
  • We have used fluorescence resonance energy transfer and co-immunoprecipitation to analyze the interactions among the α, β, and γ1 subunits of the Arabidopsis heterotrimeric G protein. (
  • Another chimeric protein MPK6-VenusN was used to test potential interactions of MPK6 with KTN1. (
  • End-binding 1 (EB1) proteins are evolutionarily conserved plus-end-tracking proteins that localize to growing microtubule plus ends where they regulate microtubule dynamics and interactions with intracellular targets. (
  • Defining specific protein interactions and spatially or temporally restricted local proteomes improves our understanding of all cellular processes, but obtaining such data is challenging, especially for rare proteins, cell types, or events. (
  • In yeasts and mammals, the interactions among centromere DNA and proteins have been extensively studied. (
  • Although architectures of PCNA-RFC complex in yeast have been revealed, the functions of PCNA and protein-protein interactions of PCNA-RFC complex in higher plants are not very clear. (
  • Here, essential regions mediating interactions between PCNA and RFC subunits in Arabidopsis and rice were investigated via yeast-two-hybrid method and bimolecular fluorescence complementation techniques. (
  • We observed that OsPCNA could interact with all OsRFC subunits, while protein-protein interactions only exist between Arabidopsis RFC2/3/4/5 and AtPCNA1/2. (
  • First, our immunoprecipitation-mass spectrometry analysis of FKF1 revealed that FKF1 forms an S-phase kinase-associated protein 1 (Skp1)/Cullin(CUL)/F-box complex through interactions with Arabidopsis Skp1-like 1 (ASK1), ASK2, and CUL1 proteins and mainly interacts with GI protein in vivo. (
  • One pfl mutant was missing the peroxisomal 3-ketoacyl-CoA thiolase encoded by the PEROXISOME DEFECTIVE1 ( PED1/At2g33150 ) gene, suggesting that peroxisomal metabolism influences the rate of matrix protein degradation. (
  • The isolation of pex6 -2 as a pfl mutant supports the hypothesis that matrix proteins can exit the peroxisome for cytosolic degradation. (
  • All three responses are affected in the ABA-insensitive mutant abi1 of Arabidopsis thaliana, suggesting that an early step in the signaling of ABA is controlled by the ABI1 locus. (
  • Storage protein gene expression is dramatically reduced in lec2 fus3 double mutant. (
  • A reduction of AtRIBA1 expression in the Arabidopsis rfd1 mutant and in RIBA1 antisense lines is not complemented by the simultaneously expressed isoforms AtRIBA2 and AtRIBA3. (
  • The expression pattern and mutant phenotype of RPS9M are similar to those of ANK6 as well, suggesting that RPS9M may work together with ANK6 in controlling female gametophyte development, possibly by regulating the expression of some mitochondrial proteins. (
  • tan mutant embryos share many characteristics with the leafy cotyledon ( lec ) class of mutants in that they accumulate anthocyanin, are intolerant of desiccation, form trichomes on cotyledons, and have reduced accumulation of storage proteins and lipids. (
  • Under non-stressed conditions the net photosynthetic rate (PN) of the mutant plants cbp20 of Arabidopsis was similar to that of the wild type (WT). (
  • Here, a yeast ( Saccharomyces cerevisiae ) SEIPIN deletion mutant strain and a plant ( Nicotiana benthamiana ) transient expression system were used to test the ability of Arabidopsis SEIPINs to influence LD morphology. (
  • Herein, a chimeric GFP-MPK6 protein expressed under the native MPK6 promoter capable of rescuing the mpk6-2 mutant phenotype was used to perform in vivo localisation studies. (
  • The second part of thesis summarises current knowledge on microtubule severing protein KTN1 with main focus on mutant phenotypes. (
  • The CO protein profile in the ztl mutant resembles that in the gi mutant, indicating that ZTL activity also may be changed in the gi mutant. (
  • DNA-binding studies with mutant AtTBP1 proteins showed that the Myb-extension domain of AtTBP1 is required for binding to plant telomeric DNA. (
  • Accumulation of isochorismate-derived 2,3-dihydroxybenzoic 3-O-beta-D-xyloside in arabidopsis resistance to pathogens and ageing of leaves. (
  • Arabidopsis SNRK2.3 protein kinase is involved in the regulation of sulfur-responsive gene expression and O-acetyl-L-serine accumulation under limited sulfur supply," Soil Science and Plant Nutrition , vol. 52, no. 2, pp. 211-220, 2006. (
  • Quantitative proteomic analysis of microtubule pull-downs, using ratiometric (14)N/(15)N labeling, revealed 605 proteins exhibiting differential accumulation during TE differentiation. (
  • The preprotein receptors Toc159 and Toc33 in Arabidopsis (Arabidopsis thaliana) are specific for the accumulation of abundant photosynthetic proteins. (
  • Further, transcript measurements of psbA and psbD-BLRP, which encode the D1 and D2 protein respectively, showed that UVR8 is involved in accumulation of psbD-BLRP transcripts but little affected psbA transcripts. (
  • Several lines of evidence including proteome profiling demonstrate the import and accumulation of proteins essential for chloroplast biogenesis and functionality. (
  • It was shown that accumulation of mitochondrial proteins was reduced in hemizygous (letm1(-/-) LETM2(+/-)) plants. (
  • Applying methods we used to uncover yeast prions, we identified nearly 500 Arabidopsis proteins that harbor potential prion-like domains (PrDs). (
  • At least one of these domains, Luminidependens PrD, had some of the classical characteristics of prion proteins when tested experimentally in yeast, making it, to our knowledge, the first protein from the plant kingdom with bona fide prion attributes. (
  • In yeast, prions heritably change protein functions by templating monomers into higher-order assemblies. (
  • In yeast, a dozen prion proteins are known ( 4 ). (
  • Although peroxisomes typically are considered to consist of a single membrane enclosing a protein lumen, more complex peroxisomal membrane structure has occasionally been observed in yeast, mammals, and plants. (
  • In addition, the potential for dimerization of the AtSERK1 protein was determined by fluorescence resonance energy transfer (FRET) between GFP-derivative-AtSERK1 fusion in plant cells and in the yeast two hybrid based protein interaction system. (
  • Each variant contained a unique relative ability to interact with Tic40 (the only verified plant rhomboid substrate) using protein pull-down methods, and demonstrated an ability to alter Mgm1 (a yeast rhomboid substrate) cleavage ratios when expressed in yeast. (
  • While investigating the ability of variant proteins to interact with Tic40 and Mgm1, an apparent physiological role within bacterial and yeast cells was uncovered. (
  • In addition, plants contain a large group of kinases related to the classical SNF1-type kinases from yeast, although the majority of these enzymes in Arabidopsis have a different primary structure compared with their yeast homologs. (
  • the details of which have come initially from yeast two-hybrid studies of the yeast and mammalian ESCRT interactomes followed by a proliferation in the past few years of biochemical, genetic, structural, and biophysical studies aimed at characterizing the role of ESCRT during MVB protein-sorting in these organisms. (
  • In the yeast two- hybrid assay, the result from the qualitative β-galactosidase filter assay showed that 6 MAPK proteins interacted with 6 MKK proteins. (
  • The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (
  • The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (
  • A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (
  • Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (
  • The lipodystrophy protein SEIPIN is important for lipid droplet ( LD ) biogenesis in human and yeast cells. (
  • On the other hand, some proteins in mammals and yeast that are involved in LD formation and turnover are conserved in plants (e.g. (
  • By protein sequence homology search using human and yeast polyadenylation factors, we identified 28 proteins that may be components of Arabidopsis polyadenylation machinery. (
  • This gene, named GPA1, was isolated by using a DNA probe generated by polymerase chain reaction based on protein sequences from mammalian and yeast G protein {alpha} subunits. (
  • In Arabidopsis thaliana , EB1a and EB1b represent one subgroup that possesses an acidic tail region reminiscent to the yeast and metazoan EB1 proteins, whereas EB1c constitutes another subgroup that contains a tail region with patches of basic amino acid residues ( supplementary material Fig. S1). (
  • Yeast DOS2 protein, involved in single-copy DNA replication and ubiquitination. (
  • These transgenic lines will be analysed to understand the function and regulation of each chloroplast Clp protein for plant growth and development. (
  • Potential differential regulation among these Clp proteins was analysed at both the mRNA and protein level. (
  • Within the various lines screened, up to 90% of ClpP4 protein content was specifically repressed, which also led to the down-regulation of ClpP3 and ClpP5 protein contents. (
  • Our results suggest that AtSKD1 contributes to vacuolar protein trafficking and thereby to the maintenance of the large central vacuole of plant cells, and might play a role in cell-cycle regulation. (
  • However, it was not clear that the DELLA proteins are involved in the GA-regulation of stamen and anther development. (
  • These observations underscore the importance of determining systematically the respective roles of the various DELLA proteins in GA-mediated regulation of Arabidopsis petal and stamen development. (
  • These studies demonstrate that regulation of ethylene biosynthesis occurs by a mechanism in which 14-3-3 proteins act through a direct interaction and stabilization of ACS and through decreasing the abundance of the ubiquitin ligases that target a subset of ACS proteins for degradation. (
  • The phenotype caused by the loss-of-function mutations suggested pivotal roles of the canonical MAPK pathway in the suppression of stomatal formation and regulation of stomatal patterning in Arabidopsis, whilst the cell type-specific manipulation of individual MAPK components revealed the existence of a positive impact on stomatal production. (
  • Thus, the deeply conserved MASS proteins are tightly connected with MAPK signaling in Arabidopsis to fine-tune stomatal production and patterning, providing a functional divergence of the YDA-MPK3/6 cascade in the regulation of plant developmental processes. (
  • These protein-protein interaction data support the potential regulation of KTN1 by MAPKs. (
  • Distinct roles of FKF1, GIGANTEA, and ZEITLUPE proteins in the regulation of CONSTANS stability in Arabidopsis photoperiodic flowering. (
  • In Arabidopsis, the day-length-dependent regulation of Constans (CO) protein stability is crucial to induce flowering locus T (FT) expression for flowering in long days. (
  • Here we demonstrated that the FKF1-interacting proteins Gigantea (GI) and Zeitlupe (ZTL) are involved in CO stability regulation. (
  • Using knowledge gained from model systems like Arabidopsis thaliana, we will investigate in the future the regulation of pollen tube growth in crop plants. (
  • In Arabidopsis , the receptor kinase CLAVATA1 (CLV1) binds to the small secreted polypeptide CLV3, and three proteins act as key elements of a signal transduction pathway that regulates shoot apical meristem maintenance. (
  • An importin ß protein negatively regulates MicroRNA activity in Arabidopsis. (
  • The phytohormone gibberellin (GA) regulates the development and fertility of Arabidopsis flowers. (
  • We show that GA regulates cell elongation rather than cell division during Arabidopsis stamen filament elongation. (
  • The Arabidopsis thaliana synaptotagmin SYTA (AT2G20990) regulates endocytosis at the plasma membrane and virus movement protein-mediated cellto-cell movement. (
  • A protein called FERONIA is found in most types of cells and regulates many of the processes that drive these responses, such as cell growth and communication between male and female cells. (
  • We report here that the Arabidopsis blue light photoreceptor CRY1 positively regulates inducible resistance to Pseudomonas syringae under continuous light conditions. (
  • Here, we report that the E2Fa transcription factor of Arabidopsis thaliana is an essential component that regulates the asymmetric cell division marking lateral root initiation. (
  • Here, we report that the miR156/SPL2 regulatory pathway regulates pollen production, fertility rate, and the elongation of floral organs, including petals, sepals, and siliques in Arabidopsis. (
  • Higher plants have the greatest number and complexity of Clp proteins than any other group of organisms, and more than 20 different Clp isomers in plants have been identified (Paper I). Because of this diversity, we have adopted a functional genomics approach to characterise all Clp proteins in the model plant Arabidopsis thaliana. (
  • However, due to their poor solubility, only a minority of integral membrane proteins have been identified, and most of them came out from in silico analyses since the genome of the model plant Arabidopsis thaliana was completed ( 2 ). (
  • Thus, the nucleoskeleton associated with the INMis needed to transmit signals to the nucleus and induce changes in chromatin organisation andultimately gene expression.A novel family of NUCLEAR ENVELOPE ASSOCIATED PROTEINS (NEAPs)proposed to be new components of the plant nucleoskeleton has been recently evidenced inthe model plant Arabidopsis thaliana. (
  • View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin , the pathway pectin degradation and in Glycan metabolism . (
  • An in silico analysis shows a correlation between the putative functions of the identified proteins and the expected roles for plasma membrane in transport, signaling, cellular traffic, and metabolism. (
  • In eukaryotes, protein kinases are involved in regulating key aspects of cellular function, including cell division, metabolism, and responses to external signals. (
  • Proteins involved in RNA homeostasis and metabolism were identified as hotspots for S-sulfenylation. (
  • The Arabidopsis somatic embryogenesis receptor kinase1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. (
  • Some proteins known to contain a BURP domain are listed below: Brassica protein BNM2, which is expressed during the induction of microspore embryogenesis. (
  • The proteins of the ELIP family were proposed to function in photoprotection or assembly of thylakoid pigment-protein complexes and are further divided into subgroups with one to three transmembrane helices. (
  • Based on these data, we propose that OHP1 and OHP2 play an essential role in the assembly or stabilization of photosynthetic pigment-protein complexes, especially photosystem reaction centers, in the thylakoid membrane. (
  • A common feature of all photosynthetic organisms is that the conversion of light energy into chemical energy is performed by large pigment-protein complexes in specialized membrane systems, the thylakoids ( Nelson and Ben-Shem, 2004 ). (
  • Assembling the photosynthetic complexes in the thylakoid membrane requires a tight coordination of protein synthesis and folding with pigment synthesis and delivery. (
  • Recently, the RLK SOBIR 1 (Suppressor Of BIR 1-1) was identified as an essential regulatory RLK of various RLPs involved in plant immunity against fungal pathogens.1 Given that SOBIR 1 appears to be a crucial component of RLP-containing complexes, we aimed to identify additional proteins interacting with SOBIR 1. (
  • The formation and scission of MVB intralumenal vesicles is unique, because, unlike other vesicle budding events (e.g., those mediated by clathrin or the coat protein complexes), it is directed away from the cytosol. (
  • We isolated the TAN gene and showed that the predicted polypeptide has seven WD repeat motifs, suggesting that TAN forms complexes with other proteins. (
  • Light energy absorbed by LHCIIs is transferred to a special pair of chlorophyll molecules in the PSII reaction center to drive electron transport though downstream protein complexes in the thylakoid membrane. (
  • The import of nucleus-encoded proteins into chloroplasts is mediated by translocon complexes in the envelope membranes. (
  • Proximity labeling enables discovery of protein neighborhoods defining functional complexes and/or organellar protein compositions. (
  • The import of cytosolic precursor proteins relies on the Toc- (translocon at the outer chloroplast membrane) and Tic- (translocon at the inner chloroplast membrane) complexes. (
  • Telomeres are specific protein-DNA complexes that protect the ends of eukaryotic chromosomes from fusion and degradation and are maintained by a specialized mechanism exerted by telomerase and telomere-binding proteins (TBPs), which are evolutionarily conserved. (
  • ) was established by clustering the predicted membrane proteins based on sequence and sorted into families of known, predicted, or unknown functions ( 3 ). (
  • In ARAMEMNON, dedicated to Arabidopsis integral membrane proteins, averaging the predictions from seven publicly available programs led to the identification of ∼6,500 proteins displaying at least one transmembrane domain (TM) of the 25,500 predicted protein sequences ( ( 4 ). (
  • In animal cells, plasma membrane proteins destined to be degraded are internalized via endocytosis and delivered through the endosomal pathway to the lysosome ( Gruenberg and Stenmark, 2004 ). (
  • To study membrane proteins is a challenging project. (
  • Park, Ohkmae 2004-10-16 00:00:00 Secreted and plasma membrane proteins play crucial roles in a variety of physiological and developmental processes of multicellular organisms. (
  • and plasma membrane proteins. (
  • In conclusion, it is proposed that AtPQL4 and AtPQL6 proteins function in retaining membrane proteins for sufficient time in the ER to allow ER-quality control and related processes to take place. (
  • The protocol has not been tested for protein import activity but is optimized for chloroplast proteome yield and purity and minimal protein degradation. (
  • We exploit developmentally timed peroxisomal content remodeling in Arabidopsis thaliana to elucidate peroxisome-associated protein degradation. (
  • We previously found that ICL is stabilized when a peroxisome-associated ubiquitin-conjugating enzyme and its membrane anchor are both mutated, suggesting that matrix proteins might exit the peroxisome for ubiquitin-dependent cytosolic degradation. (
  • To identify additional components needed for peroxisome-associated matrix protein degradation, we mutagenized a line expressing GFP-ICL, which is degraded similarly to endogenous ICL, and identified p ersistent GFP-ICL fl uorescence ( pfl ) mutants. (
  • Three possible mechanisms for peroxisomal matrix protein degradation can be envisioned: degradation within the organelle by resident proteases, degradation of the entire organelle via autophagy, or retrotranslocation out of the organelle followed by cytosolic degradation. (
  • Besides being important chaperones, many Clp/Hsp100 also participate in protein degradation by associating with the proteolytic subunit ClpP to form the Clp protease complex. (
  • 14-3-3s also interact with the ETHYLENE-OVERPRODUCER1 (ETO1)/ETO1-LIKE (EOLs), a group of three functionally redundant proteins that are components of a CULLIN-3 E3 ubiquitin ligase that target a subset of the ACS proteins for rapid degradation by the 26S proteasome. (
  • The controlled degradation of proteins by the ubiquitin-26S proteasome system is an important regulatory mechanism in eukaryotic cells. (
  • In plants, the gene families encoding the elements of the ubiquitin-26S proteasome system are greatly expanded relative to their animal counterparts ( Vierstra, 2009 ), suggesting a more prominent role for ubiquitin-based protein degradation in plant cells. (
  • In ethylene biosynthesis, a subset of 1-aminocyclopropane-1-carboxylate synthase ( ACS ) proteins, which catalyze the generally rate-limiting step in ethylene biosynthesis, are specifically targeted by ETHYLENE OVER-PRODUCER1 (ETO1) for ubiquitination and rapid degradation by the 26S proteasome. (
  • In Arabidopsis thaliana, establishment of the circadian period relies on targeted degradation of TIMING OF CAB EXPRESSION 1 (TOC1) by the 26S proteasome. (
  • These results demonstrate that CUL1 is required for TOC1 degradation and further suggest that this protein is the functional cullin for the SCF(ZTL) complex. (
  • The A-domain of Toc159 is dispensable for its in vivo activity in Arabidopsis and prone to degradation in pea ( Pisum sativum ). (
  • Mitogen-activated protein kinases (MAPK) mediate cellular signal transduction during stress responses, as well as diverse growth and developmental processes in eukaryotes. (
  • 6. Wang H, Ngwenyama N, Liu Y, Walker JC, Zhang S. Stomatal development and patterning are regulated by environmentally responsive mitogen-activated protein kinases in Arabidopsis. (
  • We begin to dissect the molecular requirements for this unexpected complexity and uncover roles for these structures in protein compartmentation and lipid mobilization. (
  • Serine/arginine-rich (SR) proteins constitute a highly conserved family of RNA-binding proteins (RBPs) with key roles in constitutive, and particularly AS, by affecting splice site selection in a concentration- and phosphorylation-dependent manner [ 2 ]. (
  • Overall, our results indicate that Arabidopsis SEIPINs are part of a conserved LD biogenesis machinery in eukaryotes and that in plants these proteins may have evolved specialized roles in the storage of neutral lipids by differentially modulating the number and sizes of lipid droplets. (
  • In order to further explore the roles of cyclic nucleotides in plants, we performed a bioinformatics based analysis of the completed Arabidopsis thaliana and Orzya sativa genomes [ 21 - 23 ] in order to elucidate the potential targets of cyclic nucleotides in plants. (
  • A family of proteins called glycosylphosphatidylinositol-anchored proteins (GPI-APs for short) play important roles in plants, animals, and other eukaryotic organisms. (
  • These findings show that FERONIA is able to perform different roles in cells by teaming up with different members of the GPI-AP family of proteins. (
  • The paralogous R3 MYB proteins CAPRICE, TRIPTYCHON and ENHANCER OF TRY AND CPC1 play pleiotropic and partly non-redundant roles in the phosphate starvation response of Arabidopsis roots. (
  • In Arabidopsis thaliana , the roles of cell division control in the development of the embryo, the shoot, and the root have been extensively studied (reviewed in references 29 and 30 ). (
  • The 180 bp family of tandem repetitive sequences, which constitutes the major centromeric satellite in Arabidopsis thaliana , is thought to play important roles in kinetochore assembly. (
  • Overexpression of Arabidopsis COP1 results in partial suppression of light-mediated development: evidence for a light-inactivable repressor of photomorphogenesis. (
  • Ectopic overexpression of SEIPIN1 in Arabidopsis resulted in increased numbers of large LDs in leaves, as well as in seeds, and increased seed oil content by up to 10% over wild-type seeds. (
  • Click here to read Isolation of chloroplast proteins from Arabidopsis thaliana for proteome analysis. (
  • Immunoblotting and proteomics revealed that components of the photosynthetic apparatus are deficient in ppi1 chloroplasts and that nonphotosynthetic chloroplast proteins are unchanged or enriched slightly. (
  • The data suggest that atToc33 is involved preferentially in the import of photosynthetic proteins and, by extension, that atToc34 is involved in the import of nonphotosynthetic chloroplast proteins. (
  • Protein-L-isoaspartate (D-aspartate) O-methyltransferases (EC that catalyze the transfer of methyl groups from S-adenosylmethionine to abnormal L-isoaspartyl and D-aspartyl residues in a variety of peptides and proteins are widely distributed in procaryotes and eucaryotes. (
  • From the analysis of tryptic peptides released from the surface of Arabidopsis thylakoids, the principal phosphoproteins were identified by matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry. (
  • To better understand the signal transduction mechanisms involving small polypeptides, we are studying 25 Arabidopsis CLV3/ESR (CLE) proteins that share a conserved C-terminal domain with CLV3 and three maize ESR proteins. (
  • The major goal of the study presented in this thesis is the biochemical characterization of the AtSERK1 protein in order to reveal some of the early steps in the signal transduction cascade mediated by AtSERK1. (
  • Microtubule interacting proteins associated with membrane trafficking, protein synthesis, DNA/RNA binding, and signal transduction peaked during secondary cell wall formation, while proteins associated with stress peaked when approaching TE cell death. (
  • In the standard model, Ca 2+ -sensor proteins, such as CaM (calmodulin), detect Ca 2+ signals and subsequently regulate downstream targets to advance the signal transduction cascade. (
  • The results reveal that these proteins, rather than being rapidly induced stress proteins, are primarily constitutive proteins that may also be involved in plant acclimation to different physiological conditions. (
  • 14-3-3 proteins are a family of conserved phospho-specific binding proteins involved in diverse physiological processes. (
  • By comparing the levels of phospho- and nonphosphopeptides, the in vivo phosphorylation states of these proteins were analyzed under different physiological conditions. (
  • The calcium-dependent protein kinase (CDPK) family is needed in plant signaling during various physiological pathways. (
  • Several physiological approaches and measurements of transcripts and protein were done using uvr8-1, sig5.1, sig5-2 and elip1/2 mutants. (
  • To demonstrate the interaction of the AtSERK1 protein with one of the potential downstream proteins, the Arabidopsis kinase associated protein phosphatase (KAPP) in vitro , phosphorylation experiments and transient transfections in cowpea mesophyll protoplasts were performed. (
  • Genome-wide biochemical analysis of Arabidopsis protein phosphatase using a wheat cell-free system," FEBS Letters , vol. 586, no. 19, pp. 3134-3141, 2012. (
  • Hypersensitive to red and blue 1 and its modification by protein phosphatase 7 are implicated in the control of Arabidopsis stomatal aperture. (
  • The study shows the effect of microbial inoculation on model plants of Arabidopsis thaliana WT-Columbia and Protein phosphatase 2A catalytic subunit mutants (C2, C2C4, C2C5) and tomato plants (Gemini original) using 1/50 Gammborg medium and 1x MS medium (with sucrose or without sucrose) respectively. (
  • Analysis of the genome revealed 7 additional proteins that are variably homologous with At1g74310 throughout the entire amino acid sequence and significant similarities or identities in the signature sequences conserved among Hsp100 proteins. (
  • AtPQL4 and AtPQL6 show high amino acid sequence identity between each other and they are the closest A. thaliana homologues of the mammalian LEC35/MPDU1 protein, which has been shown to be required for all types of Man-P-Dol dependent glycosylation in the ER. (
  • In this study, we show that OHP1 and OHP2 are highly conserved in photosynthetic eukaryotes, but have probably evolved independently and have distinct functions in Arabidopsis. (
  • Arabidopsis possesses two highly conserved proteins, cpHsc70-1 and cpHsc70-2 which are found in chloroplasts. (
  • These results indicated that Arabidopsis and rice PCNAs are highly conserved in sequence, structure and pattern of interacting with other PCNA monomer. (
  • To identify new putative transport systems, we developed a new proteomic strategy based on mass spectrometry analyses of a plasma membrane fraction enriched in hydrophobic proteins. (
  • In contrast to QUA1, another Golgi-localized protein required for HG-synthesis, QUA2 does not show sequence similarity to glycosyltransferases, but instead contains a putative methyltransferase (MT) domain. (
  • Type-2 ACS proteins have only a putative calcium-dependent protein kinase target site, and type-3 ACS proteins have a short C-terminal domain with no recognized phosphorylation sites ( Chae and Kieber, 2005 ). (
  • In a previous report, we described how plant ferredoxin-like protein (PFLP) in transgenic plants enhances resistance to bacterial pathogens associated with the hypersensitive response (HR). PFLP possesses a putative casein kinase II phosphorylation (CK2P) site at the C-terminal in which phosphorylation occurs rapidly during defense response. (
  • MPK6 can phosphorylate the MASS proteins in vitro and mutating the putative substrate sites interferes the subcellular partition and function of MASS in planta. (
  • It states that ACR9 and ACR10 proteins contain three copies of the ACT domain, whereas the ACR11 and ACR12 proteins have a putative transit peptide followed by two copies of the ACT domain. (
  • In Silico Identification of Carboxylate Clamp Type Tetratricopeptide Repeat Proteins in Arabidopsis and Rice As Putative Co-Chaperones of Hsp90/Hsp70. (
  • See 2 reference sequence protein isoforms for the GCD1 gene. (
  • Alternative splicing (AS) occurs when splice sites are differentially recognized, allowing for the production of multiple transcripts from a single gene that can potentially result in different protein isoforms. (
  • Here, we demonstrate that 14-3-3 proteins interact with multiple 1-aminocyclopropane-1-carboxylate synthase ( ACS ) isoforms in Arabidopsis thaliana . (
  • All three RIBA isoforms are shown to be translocated into chloroplasts as GFP fusion proteins. (
  • Fedoroff, Nina V. / Characterization of the Arabidopsis heterotrimeric G protein . (
  • Here we exploit the unusually large size of Arabidopsis peroxisomes to demonstrate that peroxisomes have extensive internal membranes. (
  • We use fluorescent reporter proteins that simultaneously label peroxisomal membranes and lumen to reveal abundant intralumenal vesicles (ILVs) within peroxisomes. (
  • The AtSERK1 protein is targeted to plasma membranes of plant cells. (
  • This sorting process is highly complex, involving the concentration of endocytosed membrane-bound, ubiquitinated cargo proteins within intralumenal vesicles of late endosomes or so-called multivesicular bodies (MVBs), which are derived from early (and recycling) endosomes by the progressive invagination and scission of their boundary membranes. (
  • Protein phosphorylation in each of the mutants was quantified comparatively to the wild type by mass spectrometric analyses of phosphopeptides released from the photosynthetic membranes and differentially labeled with stable isotopes. (
  • Several biochemical methods are effectively used for characterization of phosphorylated proteins in photosynthetic membranes. (
  • We propose that rapid and specific internalization of PIN proteins mediated by the DRP1 proteins and the associated CME machinery from the cell plate membranes during cytokinesis is an important mechanism for proper polar PIN positioning in interphase cells. (
  • View conserved domains detected in this protein sequence using CD-search. (
  • Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. (
  • Arabidopsis thaliana, the first plant for which the entire genome sequence is available, was also among the first plant species from which Hsp100 proteins were characterized. (
  • The Athsp101 complementary DNA (cDNA) corresponds to the gene identification At1g74310 in the Arabidopsis genome sequence. (
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (
  • Analysis of the genome sequence of the flowering plant Arabidopsis thaliana . (
  • The completed sequence of the Arabidopsis genome provides the first opportunity to identify all of the protein kinases present in a model plant. (
  • In this report, we identify all of the kinases predicted to belong to the CDPK-SnRK superfamily based on the completed Arabidopsis genome sequence ( Arabidopsis Genome Initiative, 2000 ) and examine their evolutionary origins. (
  • Nevertheless, mass spectrometry is the most effective technique when it comes to identification of exact phosphorylation site(s) in the protein sequence, which is the ultimate evidence of protein phosphorylation. (
  • RIQ1 and RIQ2 are paralogous and encode uncharacterized grana thylakoid proteins, but despite the high level of identity of the sequence, the functions of RIQ1 and RIQ2 were not redundant. (
  • The level of the ETO1/EOLs in vivo plays a role in mediating ACS protein turnover, with increased levels leading to a decrease in ACS protein levels. (
  • These mass spectrometry methods offer a new approach to assess the stoichiometry of in vivo protein phosphorylation in complex samples. (
  • The BiFC approach was used to test whether MKK1, MKK2 and MKK6 associated with MPK4 and MPK11 in vivo in Arabidopsis. (
  • Combination of reversed genetics with analyses of in vivo protein phosphorylation in Arabidopsis thaliana revealed that STN8 protein kinase is specific in phosphorylation of N-terminal threonine residues in D1, D2, and CP43 proteins, and Thr-4 in the PsbH protein of photosystem II. (
  • The same tandem mass spectrometry analysis identifies other in vivo post-translational modifications as well, such as acetylation of the N-terminus of mature protein. (
  • To investigate the effect of endospheric and rhizosphere strains (WCS714r, Sphingobium limneticum and Acidovorax delafieldii) on WT and mutants, it was discovered that WCS714r and Sphingobium limneticum and Acidovorax delafiedii strains were found to inhibit the primary root length and stimulate the lateral root formation of tomato plants, Arabidopsis WT and the mutants either in vivo or in vitro except some of endospheric and rhizosphere bacteria (Pseudomonas sp. (
  • We also demonstrate that the SPL2 protein binds to the 5′UTR of the AS2 gene in vivo, indicating that AS2 is directly regulated by SPL2. (
  • Collectively, our data indicate that changes in the phosphorylation of photosynthetic proteins are more rapid during heat stress than during normal light/ dark transitions. (
  • The extended superfamily of chlorophyll a/b binding proteins comprises the Light-Harvesting Complex Proteins (LHCs), the Early Light-Induced Proteins (ELIPs) and the Photosystem II Subunit S (PSBS). (
  • Type-1 ACS proteins contain target sites for both mitogen-activated protein kinase and calcium-dependent protein kinase phosphorylation. (
  • The use of mass spectrometry to characterize the phosphorylome, i.e. the constituents of the proteome that become phosphorylated, was demonstrated using the reversible phosphorylation of chloroplast thylakoid proteins as an example. (
  • Phosphorylation of D1, D2, and CP43 in the light-exposed leaves of two Arabidopsis lines with T-DNA insertions in the stn8 gene was found significantly reduced in the assays with anti-phosphothreonine antibodies. (
  • Phosphorylation of the PsbH protein at Thr-4 was completely abolished in the plants lacking STN8. (
  • Photosynthesis in higher plants is regulated by reversible protein phosphorylation events, in a unique light- and redox-controlled system. (
  • Protein phosphorylation is an important biological process associated with elicitor-induced defense responses in plants. (
  • many of these pathways generate H 2 O 2 , which can damage proteins. (
  • Our results suggest that the Arabidopsis CLE proteins, like CLV3, may function as secreted signaling molecules that act in diverse pathways during growth and development. (
  • Finally, to investigate the effects of nuclear structure on various stress response pathways, I moved back to Arabidopsis to assess germination rates of nuclear morphology mutants (crwn and kaku) in response to different types of abiotic stresses. (
  • Using complementary methods for the extraction of hydrophobic proteins and mass spectrometry analyses on mono-dimensional gels, about 100 proteins have been identified, 95% of which had never been found in previous proteomic studies. (
  • Here, we report on the immunopurification of a functional Arabidopsis thaliana (At)SOBIR 1-yellow fluorescent protein (YFP) fusion protein stably expressed in Arabidopsis, followed by mass-spectrometry to identify co-purifying proteins. (
  • The CDPK-SnRK superfamily consists of seven types of serine-threonine protein kinases: calcium-dependent protein kinase (CDPKs), CDPK-related kinases (CRKs), phospho enol pyruvate carboxylase kinases (PPCKs), PEP carboxylase kinase-related kinases (PEPRKs), calmodulin-dependent protein kinases (CaMKs), calcium and calmodulin-dependent protein kinases (CCaMKs), and SnRKs. (
  • Moreover, through a reverse genetics approach I show that three Arabidopsis homologs, i.e. (
  • the second type uses NDP-sugars transported into the Golgi by specific carrier proteins [ 13 , 14 ]. (
  • Mitochondrial GCD1 dysfunction reveals reciprocal cell-to-cell signaling during the maturation of Arabidopsis female gametes. (
  • Rhomboid proteins control various cellular mechanisms such as cellular development, quorum-sensing, immune system evasion, and mitochondrial membrane remodelling. (
  • Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis. (
  • Here, we report a mitochondrial ribosomal protein, RPS9M, is required for central cell maturation. (
  • To aid further understanding on the function of MPK11 and the relationship between MPK11 and interacting activators, HA-epitope tagged cDNA for MPK11 was introduced into Arabidopsis Columbia Wildtype, mpk11, mkk1 and mkk2. (
  • A comparison between different tissues showed increasing amounts of all plastid Clp proteins from roots to stems to leaves. (
  • Chapter 5 revealed that the Arabidopsis plastid rhomboid gene, At1g25290, also contains alternative splicing. (
  • Two of them - PTAC17 (plastid transcriptionally active chromosome) and CNP (crooked neck protein) were selected for further analysis. (
  • Prion proteins provide a unique mode of biochemical memory through self-perpetuating changes in protein conformation and function. (
  • Contrary to some reports, we found no biochemical or bioinformatic evidence for a plant cyclic nucleotide regulated protein kinase, suggesting that cyclic nucleotide functions in plants have evolved differently than in mammals. (
  • A reduced-function allele reveals that EARLY FLOWERING3 repressive action on the circadian clock is modulated by phytochrome signals in Arabidopsis. (
  • Halavaty A, Moffat K. Coiled-coil dimerization of the LOV2 domain of the blue-light photoreceptor phototropin 1 from Arabidopsis thaliana. (
  • The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (
  • To visualize peroxisomes, we fused bright, monomeric fluorescent reporter proteins to the targeting sequences from two peroxins (PEX proteins, the proteins necessary for peroxisome biogenesis) that function at the peroxisomal membrane. (
  • Precursor messenger RNA (pre-mRNA) splicing is an essential step in gene expression mediated by the spliceosome, a large protein complex in the cell nucleus that interacts with specific intronic sequences in the pre-mRNA called splice sites for the proper removal of introns and correct joining of exons. (
  • On the basis of two-dimensional (2-D) gel electrophoresis, Masson and Rossignol ( 5 ) estimated that 500 polypeptides were present in the PM, corresponding to about 3% of total cellular proteins identified at that time. (
  • In addition, intrinsically disordered LEA proteins contribute to cellular survival under such conditions. (
  • To address the functional homology between the two AtPQL proteins and MPDU1, the sub-cellular localisation of AtPQL4-GFP and AtPQL6-GFP fusion proteins was investigated. (
  • Prions are proteins capable of adopting profoundly different structures that possess strong functional distinction in which at least one conformation is self-perpetuating ( 1 ). (
  • Molecular chaperones mediate the correct folding and assembly of polypeptides, as well as repair damaged protein structures caused by stress, while proteases remove otherwise non-functional and potentially cytotoxic proteins. (
  • This analysis also reveals 10 proteins that display structural properties compatible with transport functions and will constitute interesting targets for further functional studies. (
  • We show also that most of the Arabidopsis ESCRT proteins examined at least partially localize to MVBs in plant cells when ectopically expressed on their own or co-expressed with other interacting ESCRT proteins, and some also induce abnormal MVB phenotypes, consistent with their proposed functional role(s) as part of the ESCRT machinery in Arabidopsis . (
  • We propose that a modification of HRB1 by PP7 under blue light is essential to acquire a proper conformation or to bring in new components for the assembly of a functional HRB1 protein complex. (
  • Control of stomatal distribution on the Arabidopsis leaf surface. (
  • The production and patterning of the stomatal lineage cells in higher plants are influenced by the activities of the widely-used mitogen-activated protein kinase (MAPK) signaling components. (
  • Here, by functionally characterizing three MAPK SUBSTRATES IN THE STOMATAL LINEAGE (MASS) proteins, we establish that they are plasma membrane-associated, positive regulators of stomatal production. (
  • Directing TurboID to stomatal nuclei enabled purification of cell type- and subcellular compartment-specific proteins. (
  • Previous analyses have shown that GA promotes the elongation of plant organs by opposing the function of the DELLA proteins, a family of nuclear growth repressors. (
  • Phylogenetic analyses imply that the monofunctional, bipartite RIBA3 proteins, which have lost DHBPS activity, evolved early in tracheophyte evolution. (
  • Moreover phenotypic analyses of selected KTN1 mutants were performed and genetically rescued these mutants by chimeric GFP-KTN1 protein expressed under the native promoter. (
  • The truncated analyses indicated that the C-terminal of Arabidopsis RFC2/3/4/5 and rice RFC1/2 is essential for binding PCNA while the region of rice RFC3/4/5 mediating interaction with PCNA distributed both at the N- and C-terminal. (
  • These studies revealed that the Dl, D2, and CP43 proteins of the photosystem II core are phosphorylated at their N-terminal threonines (Thr), the peripheral PsbH protein is phosphorylated at Thr-2, and the mature light-harvesting polypeptides LCHII are phosphorylated at Thr-3. (
  • Successful import of hundreds of nucleus-encoded proteins is essential for chloroplast biogenesis. (
  • Selective trafficking of KNOTTED1 homeodomain protein and its mRNA through plasmodesmata. (
  • The increases in protein were mirrored at the mRNA level for most ClpP isomers but not for ClpC1, ClpC2 and ClpD and ClpP5, which exhibited little change in transcript levels. (
  • The polyadenylation process requires two components, the cis -elements on the mRNA and a group of protein factors that recognize the cis -elements and produce the poly(A) tail. (
  • Here we report a comprehensive pairwise protein-protein interaction mapping and gene expression profiling of the mRNA polyadenylation protein machinery in Arabidopsis. (
  • The inventory of the plasma membrane proteome generated by this approach contains numerous plasma membrane integral proteins, one-third displaying at least four transmembrane segments. (
  • The total number of ∼750 PM proteins can now be inferred from the entire Arabidopsis proteome ( 2 ). (
  • Proteome differences in ppi1 could be correlated with protein import rates: ppi1 chloroplasts imported the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit and 33-kD oxygen-evolving complex precursors at significantly reduced rates, but the import of a 50S ribosomal subunit precursor was largely unaffected. (
  • Genetic analysis shows that the Arabidopsis DELLA proteins RGA and RGL2 jointly repress petal, stamen and anther development in GA-deficient plants, and that this function is enhanced by RGL1 activity. (
  • Substrate adaptors, such as the ETO1/EOLs, regulate the accessibility of substrate proteins to the CUL3 ligase for ubiquitination, and their availability is important for the ubiquitination and, thus, stability of the cognate substrates. (
  • Two closely related subclass II SnRK2 protein kinases cooperatively regulate drought-inducible gene expression," Plant and Cell Physiology , vol. 51, no. 5, pp. 842-847, 2010. (
  • Altogether, proteins linking microtubules to a range of metabolic compartments vary specifically during TE differentiation and regulate different aspects of wall patterning. (
  • These results suggest that CRY1 may positively regulate R protein-mediated resistance to P. syringae with increased PR gene expression. (
  • The essential eukaryotic molecular chaperone Hsp90 operates with the help of different co-chaperones, which regulate its ATPase activity and serve as adaptors to recruit client proteins and other molecular chaperones, such as Hsp70, to the Hsp90 complex. (
  • Three partly redundant single R3 MYB proteins, CAPRICE (CPC), ENHANCER OF TRY AND CPC1 (ETC1) and TRIPTYCHON (TRY), positively regulate the root hair cell fate by. (
  • We found that Arabidopsis RALF4 and RALF19 redundantly regulate pollen tube integrity and growth, and that their function depends on pollen-expressed proteins of the LEUCINE-RICH REPEAT EXTENSIN (LRX) family, which play a role in cell wall development but whose mode of action is not understood. (
  • In molecular biology, the BSD domain is an approximately 60-amino-acid-long protein domain named after the BTF2-like transcription factors, synapse-associated proteins and DOS2-like proteins in which it is found. (
  • In molecular biology, the BURP domain is a ~230-amino acid protein domain, which has been named for the four members of the group initially identified, BNM2, USP, RD22, and PG1beta. (
  • Indeed, the hydrophobic protein equipment of plant plasma membrane still remains largely unknown, though several proteomic approaches have been reported. (
  • We produced from Arabidopsis cell suspensions a highly purified plasma membrane fraction and characterized it in detail by immunological and enzymatic tests. (
  • Furthermore, our data indicate that the MASS proteins may directly interact with the MAPKK Kinase YODA (YDA) at the plasma membrane. (
  • A second set of experiments employed YFP-fusion proteins of AtSYP121, a plasma membrane SNARE protein, and AtTIP2, a tonoplast aquaporin. (
  • Confocal microscopy confirmed plasma membrane/tonoplast localisation of the YFP proteins when expressed on their own in tobacco leaf cells. (
  • Basu D, Tian L, Debrosse T et al (2016) Glycosylation of a fasciclin-like arabinogalactan-protein (SOS5) mediates root growth and seed mucilage adherence via a cell wall receptor-like kinase (FEI1/FEI2) pathway in Arabidopsis . (