Aquaporin 1: Aquaporin 1 forms a water-specific channel that is constitutively expressed at the PLASMA MEMBRANE of ERYTHROCYTES and KIDNEY TUBULES, PROXIMAL. It provides these cells with a high permeability to WATER. In humans polymorphisms of this protein result in the Colton blood group antigen.Aquaporin 5: Aquaporin 5 is a water-specific channel protein that is expressed primarily in alveolar, tracheal, and upper bronchial EPITHELIUM. It plays an important role in maintaining water HOMEOSTASIS in the LUNGS and may also regulate release of SALIVA and TEARS in the SALIVARY GLANDS and the LACRIMAL GLAND.Aquaporin 3: Aquaporin 3 is an aquaglyceroporin that is expressed in the KIDNEY COLLECTING DUCTS and is constitutively localized at the basolateral MEMBRANE.Aquaporin 4: Aquaporin 4 is the major water-selective channel in the CENTRAL NERVOUS SYSTEM of mammals.Aquaporins: A class of porins that allow the passage of WATER and other small molecules across CELL MEMBRANES.Aquaporin 2: Aquaporin 2 is a water-specific channel protein that is expressed in KIDNEY COLLECTING DUCTS. The translocation of aquaporin 2 to the apical PLASMA MEMBRANE is regulated by VASOPRESSIN, and MUTATIONS in AQP2 have been implicated in a variety of kidney disorders including DIABETES INSIPIDUS.Aquaporin 6: Aquaporin 6 is an aquaglyceroporin that is found primarily in KIDNEY COLLECTING DUCTS. AQP6 protein functions as an anion-selective channel.Water: A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Mercuric Chloride: Mercury chloride (HgCl2). A highly toxic compound that volatizes slightly at ordinary temperature and appreciably at 100 degrees C. It is corrosive to mucous membranes and used as a topical antiseptic and disinfectant.Osmosis: Tendency of fluids (e.g., water) to move from the less concentrated to the more concentrated side of a semipermeable membrane.Aquaglyceroporins: A subgroup of aquaporins that transport WATER; GLYCEROL; and other small solutes across CELL MEMBRANES.Blood Group Antigens: Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.Water-Electrolyte Balance: The balance of fluid in the BODY FLUID COMPARTMENTS; total BODY WATER; BLOOD VOLUME; EXTRACELLULAR SPACE; INTRACELLULAR SPACE, maintained by processes in the body that regulate the intake and excretion of WATER and ELECTROLYTES, particularly SODIUM and POTASSIUM.Glycerol: A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.Permeability: Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.Cell Membrane Permeability: A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.Plant Transpiration: The loss of water vapor by plants to the atmosphere. It occurs mainly from the leaves through pores (stomata) whose primary function is gas exchange. The water is replaced by a continuous column of water moving upwards from the roots within the xylem vessels. (Concise Dictionary of Biology, 1990)Kidney Tubules, Collecting: Straight tubes commencing in the radiate part of the kidney cortex where they receive the curved ends of the distal convoluted tubules. In the medulla the collecting tubules of each pyramid converge to join a central tube (duct of Bellini) which opens on the summit of the papilla.Kidney Concentrating Ability: The ability of the kidney to excrete in the urine high concentrations of solutes from the blood plasma.Diabetes Insipidus, Nephrogenic: A genetic or acquired polyuric disorder characterized by persistent hypotonic urine and HYPOKALEMIA. This condition is due to renal tubular insensitivity to VASOPRESSIN and failure to reduce urine volume. It may be the result of mutations of genes encoding VASOPRESSIN RECEPTORS or AQUAPORIN-2; KIDNEY DISEASES; adverse drug effects; or complications from PREGNANCY.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Ion Channels: Gated, ion-selective glycoproteins that traverse membranes. The stimulus for ION CHANNEL GATING can be due to a variety of stimuli such as LIGANDS, a TRANSMEMBRANE POTENTIAL DIFFERENCE, mechanical deformation or through INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS.Neuromyelitis Optica: A syndrome characterized by acute OPTIC NEURITIS; MYELITIS, TRANSVERSE; demyelinating and/or necrotizing lesions in the OPTIC NERVES and SPINAL CORD; and presence of specific autoantibodies to AQUAPORIN 4.Polyuria: Urination of a large volume of urine with an increase in urinary frequency, commonly seen in diabetes (DIABETES MELLITUS; DIABETES INSIPIDUS).Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Xenopus laevis: The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.Osmotic Pressure: The pressure required to prevent the passage of solvent through a semipermeable membrane that separates a pure solvent from a solution of the solvent and solute or that separates different concentrations of a solution. It is proportional to the osmolality of the solution.Oocytes: Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).Antidiuretic Agents: Agents that reduce the excretion of URINE, most notably the octapeptide VASOPRESSINS.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Tulipa: A plant genus of the family LILIACEAE. Members contain tuliposides and tulipalins and have been associated with allergic contact dermatitis in florists.Vapor Pressure: The contribution to barometric PRESSURE of gaseous substance in equilibrium with its solid or liquid phase.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cistaceae: A plant family of the order Violales, subclass Dilleniidae, class Magnoliopsida. The common name of rock rose is used with several plants of this family.Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)Brain Edema: Increased intracellular or extracellular fluid in brain tissue. Cytotoxic brain edema (swelling due to increased intracellular fluid) is indicative of a disturbance in cell metabolism, and is commonly associated with hypoxic or ischemic injuries (see HYPOXIA, BRAIN). An increase in extracellular fluid may be caused by increased brain capillary permeability (vasogenic edema), an osmotic gradient, local blockages in interstitial fluid pathways, or by obstruction of CSF flow (e.g., obstructive HYDROCEPHALUS). (From Childs Nerv Syst 1992 Sep; 8(6):301-6)Mercury Compounds: Inorganic compounds that contain mercury as an integral part of the molecule.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Lens, Crystalline: A transparent, biconvex structure of the EYE, enclosed in a capsule and situated behind the IRIS and in front of the vitreous humor (VITREOUS BODY). It is slightly overlapped at its margin by the ciliary processes. Adaptation by the CILIARY BODY is crucial for OCULAR ACCOMMODATION.Gene Expression Regulation, Plant: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.Eye ProteinsBody Water: Fluids composed mainly of water found within the body.Renal Agents: Drugs used for their effects on the kidneys' regulation of body fluid composition and volume. The most commonly used are the diuretics. Also included are drugs used for their antidiuretic and uricosuric actions, for their effects on the kidneys' clearance of other drugs, and for diagnosis of renal function.AnguillaOsmolar Concentration: The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.Vasopressins: Antidiuretic hormones released by the NEUROHYPOPHYSIS of all vertebrates (structure varies with species) to regulate water balance and OSMOLARITY. In general, vasopressin is a nonapeptide consisting of a six-amino-acid ring with a cysteine 1 to cysteine 6 disulfide bridge or an octapeptide containing a CYSTINE. All mammals have arginine vasopressin except the pig with a lysine at position 8. Vasopressin, a vasoconstrictor, acts on the KIDNEY COLLECTING DUCTS to increase water reabsorption, increase blood volume and blood pressure.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Dehydration: The condition that results from excessive loss of water from a living organism.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Glomeromycota: A phylum of fungi that are mutualistic symbionts and form ARBUSCULAR MYCORRHIZAE with PLANT ROOTS.Microscopy, Electron, Scanning Transmission: A type of TRANSMISSION ELECTRON MICROSCOPY in which the object is examined directly by an extremely narrow electron beam scanning the specimen point-by-point and using the reactions of the electrons that are transmitted through the specimen to create the image. It should not be confused with SCANNING ELECTRON MICROSCOPY.Salivary Glands: Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).Mycelium: The body of a fungus which is made up of HYPHAE.Deamino Arginine Vasopressin: A synthetic analog of the pituitary hormone, ARGININE VASOPRESSIN. Its action is mediated by the VASOPRESSIN receptor V2. It has prolonged antidiuretic activity, but little pressor effects. It also modulates levels of circulating FACTOR VIII and VON WILLEBRAND FACTOR.Propylene Glycol: A clear, colorless, viscous organic solvent and diluent used in pharmaceutical preparations.Mesophyll Cells: Large and highly vacuolated cells possessing many chloroplasts occuring in the interior cross-section of leaves, juxtaposed between the epidermal layers.Mesembryanthemum: A plant genus of the family AIZOACEAE. It is a native of Africa and widely planted for erosion control to stabilize soil along roadsides and beaches.Kidney Medulla: The internal portion of the kidney, consisting of striated conical masses, the renal pyramids, whose bases are adjacent to the cortex and whose apices form prominent papillae projecting into the lumen of the minor calyces.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Submandibular Gland: One of two salivary glands in the neck, located in the space bound by the two bellies of the digastric muscle and the angle of the mandible. It discharges through the submandibular duct. The secretory units are predominantly serous although a few mucous alveoli, some with serous demilunes, occur. (Stedman, 25th ed)Receptors, Vasopressin: Specific molecular sites or proteins on or in cells to which VASOPRESSINS bind or interact in order to modify the function of the cells. Two types of vasopressin receptor exist, the V1 receptor in the vascular smooth muscle and the V2 receptor in the kidneys. The V1 receptor can be subdivided into V1a and V1b (formerly V3) receptors.Droughts: Prolonged dry periods in natural climate cycle. They are slow-onset phenomena caused by rainfall deficit combined with other predisposing factors.Astrocytes: A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Xenopus: An aquatic genus of the family, Pipidae, occurring in Africa and distinguished by having black horny claws on three inner hind toes.Mercury: A silver metallic element that exists as a liquid at room temperature. It has the atomic symbol Hg (from hydrargyrum, liquid silver), atomic number 80, and atomic weight 200.59. Mercury is used in many industrial applications and its salts have been employed therapeutically as purgatives, antisyphilitics, disinfectants, and astringents. It can be absorbed through the skin and mucous membranes which leads to MERCURY POISONING. Because of its toxicity, the clinical use of mercury and mercurials is diminishing.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Nobel PrizeRats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Spinacia oleracea: A widely cultivated plant, native to Asia, having succulent, edible leaves eaten as a vegetable. (From American Heritage Dictionary, 1982)Glycerol Kinase: An enzyme that catalyzes the formation of glycerol 3-phosphate from ATP and glycerol. Dihydroxyacetone and L-glyceraldehyde can also act as acceptors; UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway. EC 2.7.1.30.Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.Pichia: Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.Fragaria: A plant genus of the family ROSACEAE known for the edible fruit.Rats, Brattleboro: A mutant strain of Rattus norvegicus used in research on renal function and hypertension and as a disease model for diabetes insipidus.Plant Stomata: Closable openings in the epidermis of plants on the underside of leaves. They allow the exchange of gases between the internal tissues of the plant and the outside atmosphere.Proteolipids: Protein-lipid combinations abundant in brain tissue, but also present in a wide variety of animal and plant tissues. In contrast to lipoproteins, they are insoluble in water, but soluble in a chloroform-methanol mixture. The protein moiety has a high content of hydrophobic amino acids. The associated lipids consist of a mixture of GLYCEROPHOSPHATES; CEREBROSIDES; and SULFOGLYCOSPHINGOLIPIDS; while lipoproteins contain PHOSPHOLIPIDS; CHOLESTEROL; and TRIGLYCERIDES.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Saline Solution, Hypertonic: Hypertonic sodium chloride solution. A solution having an osmotic pressure greater than that of physiologic salt solution (0.9 g NaCl in 100 ml purified water).Antimony Potassium Tartrate: A schistosomicide possibly useful against other parasites. It has irritant emetic properties and may cause lethal cardiac toxicity among other adverse effects.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Malpighian Tubules: Slender tubular or hairlike excretory structures found in insects. They emerge from the alimentary canal between the mesenteron (midgut) and the proctodeum (hindgut).Glial Fibrillary Acidic Protein: An intermediate filament protein found only in glial cells or cells of glial origin. MW 51,000.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.RNA, Complementary: Synthetic transcripts of a specific DNA molecule or fragment, made by an in vitro transcription system. This cRNA can be labeled with radioactive uracil and then used as a probe. (King & Stansfield, A Dictionary of Genetics, 4th ed)Immunoblotting: Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.PhloretinMicroscopy, Immunoelectron: Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Freezing: Liquids transforming into solids by the removal of heat.Solute Carrier Family 12, Member 1: Na-K-Cl transporter in the ASCENDING LIMB OF LOOP OF HENLE. It mediates active reabsorption of sodium chloride and is inhibited by LOOP DIURETICS such as FUROSEMIDE; and BUMETANIDE. Mutations in the gene encoding SLC12A1 are associated with a BARTTER SYNDROME.4-Chloromercuribenzenesulfonate: A cytotoxic sulfhydryl reagent that inhibits several subcellular metabolic systems and is used as a tool in cellular physiology.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Urea: A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.Hypertonic Solutions: Solutions that have a greater osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.Arabidopsis: A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Cell Size: The quantity of volume or surface area of CELLS.Morula: An early embryo that is a compact mass of about 16 BLASTOMERES. It resembles a cluster of mulberries with two types of cells, outer cells and inner cells. Morula is the stage before BLASTULA in non-mammalian animals or a BLASTOCYST in mammals.Sodium-Potassium-Chloride Symporters: A subclass of symporters that specifically transport SODIUM CHLORIDE and/or POTASSIUM CHLORIDE across cellular membranes in a tightly coupled process.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Quercus: A plant genus of the family FAGACEAE that is a source of TANNINS. Do not confuse with Holly (ILEX).Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Zea mays: A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.Molecular Dynamics Simulation: A computer simulation developed to study the motion of molecules over a period of time.Acclimatization: Adaptation to a new environment or to a change in the old.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Hydroxyethyl Starch Derivatives: Starches that have been chemically modified so that a percentage of OH groups are substituted with 2-hydroxyethyl ether groups.Sodium Chloride: A ubiquitous sodium salt that is commonly used to season food.

Defective secretion of saliva in transgenic mice lacking aquaporin-5 water channels. (1/171)

Aquaporin-5 (AQP5) is a water-selective transporting protein expressed in epithelial cells of serous acini in salivary gland. We generated AQP5 null mice by targeted gene disruption. The genotype distribution from intercross of founder AQP5 heterozygous mice was 70:69:29 wild-type:heterozygote:knockout, indicating impaired prenatal survival of the null mice. The knockout mice had grossly normal appearance, but grew approximately 20% slower than litter-matched wild-type mice when placed on solid food after weaning. Pilocarpine-stimulated saliva production was reduced by more than 60% in AQP5 knockout mice. Compared with the saliva from wild-type mice, the saliva from knockout mice was hypertonic (420 mosM) and dramatically more viscous. Amylase and protein secretion, functions of salivary mucous cells, were not affected by AQP5 deletion. Water channels AQP1 and AQP4 have also been localized to salivary gland; however, pilocarpine stimulation studies showed no defect in the volume or composition of saliva in AQP1 and AQP4 knockout mice. These results implicate a key role for AQP5 in saliva fluid secretion and provide direct evidence that high epithelial cell membrane water permeability is required for active, near-isosmolar fluid transport.  (+info)

Lung fluid transport in aquaporin-5 knockout mice. (2/171)

The mammalian lung expresses water channel aquaporin-1 (AQP1) in microvascular endothelia, AQP4 in airway epithelia, and AQP5 at the apical plasma membrane in type I cells of alveolar epithelia. We previously studied the role of AQP1 and AQP4 in lung fluid transport using knockout mice. Here, we examined the role of AQP5 using AQP5 knockout mice, which were recently shown to manifest defective saliva secretion. AQP5 deletion did not affect lung morphology at the light microscopic level, nor did it affect the distribution or expression of aquaporins 1, 3, or 4. Airspace-capillary osmotic water permeability (P(f)) was measured in isolated perfused lungs by pleural surface fluorescence and gravimetric methods. P(f) was reduced 10-fold by AQP5 deletion and was further reduced by 2- to 3-fold in AQP1/AQP5 double-knockout mice. Hydrostatic lung edema in response to acute increases in pulmonary artery pressure was not affected by AQP5 deletion. Active alveolar fluid absorption was measured in an in situ lung model from the increase in concentration of a volume marker in an isosmolar alveolar instillate. Interestingly, fluid absorption did not differ in litter-matched AQP5 knockout mice, nor was there an effect of AQP5 deletion when fluid absorption was maximally stimulated by pretreatment of mice with keratinocyte growth factor. These results indicate that AQP5 is responsible for the majority of water transport across the apical membrane of type I alveolar epithelial cells. The unimpaired alveolar fluid clearance in AQP5-null mice indicates that high alveolar water permeability is not required for active, near-isosmolar fluid transport.  (+info)

Hypertonic induction of aquaporin-5 expression through an ERK-dependent pathway. (3/171)

Aquaporin-5 (AQP5) is a water channel protein expressed in lung, salivary gland, and lacrimal gland epithelia. Each of these sites may experience fluctuations in surface liquid osmolarity; however, osmotic regulation of AQP5 expression has not been reported. This study demonstrates that AQP5 is induced by hypertonic stress and that induction requires activation of extracellular signal-regulated kinase (ERK). Incubation of mouse lung epithelial cells (MLE-15) in hypertonic medium produced a dose-dependent increase in AQP5 expression; AQP5 protein peaked by 24 h and returned to baseline levels within hours of returning cells to isotonic medium. AQP5 induction was observed only with relatively impermeable solutes, suggesting an osmotic pressure gradient is required for induction. ERK was selectively activated in MLE-15 cells by hypertonic stress, and inhibition of ERK activation with two distinct mitogen-activated extracellular regulated kinase kinase (MEK) inhibitors, U0126 and PD98059, blocked AQP5 induction. AQP5 induction was also observed in the lung, salivary, and lacrimal glands of hyperosmolar rats, suggesting potential physiologic relevance for osmotic regulation of AQP5 expression. This report provides the first example of hypertonic induction of an extrarenal aquaporin, as well as the first association between mitogen-activated protein kinase signaling and aquaporin expression.  (+info)

Conditional expression of fibroblast growth factor-7 in the developing and mature lung. (4/171)

Effects of fibroblast growth factor-7 (FGF-7) on lung morphogenesis, respiratory epithelial cell differentiation, and proliferation were assessed in transgenic mice in which the human FGF-7 cDNA was controlled by a conditional promoter under the direction of regulatory elements from either the human surfactant protein-C (SP-C) or rat Clara cell secretory protein (ccsp) genes. Expression of FGF-7 was induced in respiratory epithelial cells of the fetal lung by administration of doxycycline to the dam. Prenatally, doxycycline induced FGF-7 mRNA in respiratory epithelial cells in both Sp-c and Ccsp transgenic lines, increasing lung size and causing cystadenomatoid malformation. Postnatally, mice bearing both Ccsp-rtta and (Teto)(7)-cmv-fgf-7 transgenes survived, and lung morphology was normal. Induction of FGF-7 expression by doxycycline in the Ccsp-rtta x (Teto)(7)-cmv-fgf-7 mice caused marked epithelial cell proliferation, adenomatous hyperplasia, and pulmonary infiltration with mononuclear cells. Epithelial cell hyperplasia caused by FGF-7 was largely resolved after removal of doxycycline. Surfactant proteins, TTF-1, and aquaporin 5 expression were conditionally induced by doxycycline. The Sp-c-rtta and Ccsp-rtta activator mice provide models in which expression is conditionally controlled in respiratory epithelial cells in the developing and mature lung, altering lung morphogenesis, differentiation, and proliferation.  (+info)

Differential regulation of rat aquaporin-5 promoter/enhancer activities in lung and salivary epithelial cells. (5/171)

Aquaporin-5 (AQP5) is a water channel protein that is selectively expressed in respiratory, salivary, and lacrimal tissues. In order to establish the tissue-specific transcriptional programs that underlie its lung- and salivary-specific expression, a 4.5-kilobase pair DNA fragment encompassing the 5'-flanking region of the rat AQP5 gene has been characterized in detail. A major transcription start site utilized in lung and salivary glands has been localized downstream of a TATAA-like motif. Transient transfection assays of -4.3- and -1.7-AQP5-luciferase constructs in AQP5-expressing lung (MLE-15) and salivary (Pa-4) cells and nonexpressing fibroblast (NIH3T3) and epithelial (HeLa) cells demonstrate preferential transcriptional enhancement of reporter activities in MLE-15 and Pa-4 cells. Transient transfection assays of a series of 5' --> 3' deletion constructs of -4.3-AQP5-luciferase suggest that a common salivary and lung enhancer is located between nucleotides -274 and -139, and a lung-specific enhancer is located between nucleotides -894 and -710. There is one putative lung-specific repressor located in the region of nucleotides -1003/-894 and a common lung and salivary repressor located at nucleotides -503/-385. Moreover, 3' --> 5' deletions up to -171 and -127 base pairs almost abolish transcriptional activation in salivary and lung cells, respectively. Together, our findings indicate that the combination of enhancer/repressor elements within the proximal 5'-flanking region of rat AQP5 gene dictates its restricted expression in both lung and salivary cells.  (+info)

Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice. (6/171)

Aquaporin (AQP) water channels provide a major pathway for osmotically driven water movement across epithelial and microvascular barriers in the lung. We used mice deficient in each of the three principal lung aquaporins, AQP1, AQP4 and AQP5, to test the hypothesis that aquaporins are important in neonatal lung fluid balance, adult lung fluid clearance and formation of lung oedema after acute lung injury. Wet-to-dry weight ratios (W/D) in lungs from wild-type mice decreased from 7.9 to 5.7 over the first hour after spontaneous delivery. AQP deletion did not significantly affect W/D at 45 min after birth. Alveolar fluid clearance was measured in living ventilated mice in which 0.5 ml saline containing radiolabelled albumin was instilled into the airspaces. Fluid clearance was 17.4 % in 15 min and inhibited >90 % by amiloride, but clearance was not affected by AQP deletion. W/D was measured in established models of acute lung injury - acid aspiration and thiourea administration. Two hours after intratracheal administration of HCl, W/D increased from 3.7 to 7.5 but was not affected by AQP deletion. Three hours after intraperitoneal infusion of thiourea, W/D increased to 5.5 and marked pleural effusions appeared, but there were no differences in wild-type and AQP knockout mice. Hyperoxic subacute lung injury was induced by 95 % oxygen. Neither mean survival (143 h) nor W/D at 65 h (5.1) were significantly affected by AQP deletion. Despite their role in osmotically driven lung water transport, aquaporins are not required for the physiological clearance of lung water in the neonatal or adult lung, or for the accumulation of extravascular lung water in the injured lung.  (+info)

Persistent increase in the amount of aquaporin-5 in the apical plasma membrane of rat parotid acinar cells induced by a muscarinic agonist SNI-2011. (7/171)

SNI-2011 induces the long-lasting increase in the amount of aquaporin-5 (AQP5) in apical plasma membranes (APMs) of rat parotid acini in a concentration-dependent manner. This induction was inhibited by p-F-HHSiD, U73122, TMB-8, or dantrolene but not by bisindolmaleimide or H-7, indicating that SNI-2011 acting at M(3) muscarinic receptors induced translocation of AQP5 via [Ca(2+)](i) elevation but not via the activation of protein kinase C. In contrast, acetylcholine induced a transient translocation of AQP5 to APMs. SNI-2011 induces long-lasting oscillations of [Ca(2+)](i) in the presence of extracellular Ca(2+). Thus, SNI-2011 induces a long-lasting translocation of AQP5 to APMs coupled with persistent [Ca(2+)](i) oscillations.  (+info)

Aquaporin water channel in salivary glands. (8/171)

Water secretion from salivary glands, which are innervated by parasympathetic and sympathetic nerves, occurs in response to the stimulation by neurotransmitters. In general, parasympathetic or sympathetic stimulation produces a high flow of saliva as a result of the activation of M3 muscarinic or alpha1-adrenergic receptors, respectively. The secretory mechanisms of fluid secretion were osmotically regulated in response to a transepithelial ion gradient generated by ion transport systems that were located in the apical or basolateral membranes of the acinar cells. Recently, the identification of water-specific channels, or aquaporins (AQPs), in many mammalian tissue and cell types has provided insight into the molecular basis of water movement across biological membranes. It has been reported that several AQPs are expressed in salivary glands and especially AQP5 plays an important role in fluid secretion. This review will focus on the role of AQP5 in the movement of water across the apical plasma membrane in relation to the physiology and pathophysiology of salivary glands.  (+info)

*AQP5

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 ... Aquaporin-5 is a protein that in humans is encoded by the AQP5 gene. Aquaporin 5 (AQP5) is a water channel protein. ... "Entrez Gene: AQP5 aquaporin 5". Verkman AS (2003). "Role of aquaporin water channels in eye function". Exp. Eye Res. 76 (2): ... 2003). "Distribution of aquaporin water channels AQP1 and AQP5 in the ductal system of the human pancreas". Gut. 52 (7): 1008- ...

*GATA6

... also indicated in proper lung development by controlling the late differentiation stages of alveolar epithelium and aquaporin-5 ... 275 (33): 25330-5. doi:10.1074/jbc.M000828200. PMID 10851229. Crawford SE, Qi C, Misra P, Stellmach V, Rao MS, Engel JD, Zhu Y ... 277 (5): 3585-92. doi:10.1074/jbc.M107995200. PMID 11724781. Liu C, Glasser SW, Wan H, Whitsett JA (2002). "GATA-6 and thyroid ... "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1-2): 149-56. doi: ...

*KC6

... a novel cornea-expressed gene and the absence of transcripts for aquaporin 5" (PDF). Invest. Ophthalmol. Vis. Sci. 46 (4): 1239 ...

*Focal hyperhidrosis

Based on previous research using mice and rats, researchers looked towards the role of aquaporin 5 (AQP5), a water channel ... Gresz, V.; Kwon, T; Gong, H; Agre, P.; Steward, M; King, L; Nielsen, S. (2004). "Immunolocalization of AQP-5 in rat parotid and ... 26 (4): 460-5. doi:10.1097/mop.0000000000000108. PMID 24905102. Singh, S; Davis, H; Wilson, P (October 2015). "Axillary ...

*Sweat gland

... can sometimes come with abnormal aquaporin 5 in the sweat glands. Cystic fibrosis can be diagnosed by a sweat test, as the ... 5 (1): 35-36. doi:10.1111/j.1600-0560.1978.tb00935.x. ISSN 1600-0560. James, Berger & Elston 2011, p. 534. Rubin & Strayer 2011 ... 25 (5): 761-767. doi:10.1172/JCI101760. ISSN 0021-9738. PMC 435616 . PMID 16695370. Retrieved 18 December 2012. Caceci, Thomas ... 100 (5): 1692-1701. doi:10.1152/japplphysiol.01124.2005. ISSN 8750-7587. PMID 16614366. Sørensen, Vibeke W.; Prasad, Gaya (1973 ...

*Uterine epithelium

Lindsay, L. A., & Murphy, C. R. (2006). Redistribution of aquaporins 1 and 5 in the rat uterus is dependent on progesterone: a ... Lindsay, L. A., & Murphy, C. R. (2004). Redistribution of aquaporins in uterine epithelial cells at the time of implantation in ...

*Syndrome of inappropriate antidiuretic hormone secretion

... via activation of aquaporins, the site of the ADH receptors - back into the circulation. This has two consequences. First, in ... aquaporin 2), and their insertion into the cells' luminal membranes. Excessive ADH causes an inappropriate increase in the ... "Physiology and pathophysiology of renal aquaporins". Seminars in nephrology. 21 (3): 231-8. doi:10.1053/snep.2001.21647. PMID ... inherited mutations that cause aquaporins always to be "turned on"; and 6) miscellaneous largely transient conditions. ...

*SKIP

24 (4): 1001-5. doi:10.1042/bst0241001. PMID 8968500. Mitchell CA, Brown S, Campbell JK, Munday AD, Speed CJ (Nov 1996). " ... The inositol 5-phosphatase skip localizes to the endoplasmic reticulum and translocates to membrane ruffles following epidermal ... This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in ... "The inositol Inpp5k 5-phosphatase affects osmoregulation through the vasopressin-aquaporin 2 pathway in the collecting system ...

*Aquaporin 4

The expression of aquaporin 4 is reliant on the disease stage of TBI. In an acute stage of TBI, the lack of aquaporin 4 causes ... Aquaporin-4, also known as AQP4, is a water channel protein encoded by the AQP4 gene in humans. AQP4 belongs to the aquaporin ... Aquaporin-4 is essential in the formation of memory as well as synaptic plasticity. Other performances that aquaporin-4 is ... "Aquaporin-4 autoimmune syndrome and anti-aquaporin-4 antibody-negative opticospinal multiple sclerosis in Japanese". Multiple ...

*Aquaporin

The gating of an aquaporin is carried out by an interaction between a gating mechanism and the aquaporin, which causes a 3D ... It was not until 1992 that the first aquaporin, 'aquaporin-1' (originally known as CHIP 28), was reported by Peter Agre, of ... There have been two clear examples of diseases identified as resulting from mutations in aquaporins: Mutations in the aquaporin ... aquaporins. In addition to the maintenance of normal cytosolic osmolarity, aquaporins can play a major role in extension growth ...

*Aquaporin 3

"Aquaporin-2 and -3: representatives of two subgroups of the aquaporin family colocalized in the kidney collecting duct". Annu. ... Aquaporin 3 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to ... 2005). "Roles of aquaporin-3 water channels in volume-regulatory water flow in a human epithelial cell line". J. Membr. Biol. ... 2007). "Expression of aquaporin 3 in the human prostate". Int. J. Urol. 14 (12): 1088-92; discussion 1092. doi:10.1111/j.1442- ...

*Aquaporin 2

It is the only aquaporin regulated by vasopressin. The basic job of aquaporin 2 is to reabsorb water from the urine while its ... This aquaporin is also regulated by food intake. Fasting reduces expression of this aquaporin independently of vasopressin. ... so the aquaporin 2 can be used by the cell. This aquaporin is regulated in two ways by the peptide hormone vasopressin: short- ... Aquaporin 2 is in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed, ...

*Aquaporin 1

... at the US National Library of Medicine Medical Subject Headings (MeSH) Gallery of Aquaporin Simulations Human AQP1 ... The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Aquaporin ... AQP1 aquaporin 1 (Colton blood group)". Knepper MA (1994). "The aquaporin family of molecular water channels". Proc. Natl. Acad ... Aquaporin 1 is a protein that in humans is encoded by the AQP1 gene. AQP1 is a widely expressed water channel, whose ...

*Marine life

Agre, P (2006). "The aquaporin water channels". Proceedings of the American Thoracic Society. 3 (1): 5-13. doi:10.1513/pats. ... 19 (6): 5-24. Borgonie G, García-Moyano A, Litthauer D, Bert W, Bester A, van Heerden E, Möller C, Erasmus M, Onstott TC (June ... Retrieved 5 August 2016. Lee, H. D. P. (1948) "Place-Names and the date of Aristotle's Biological Works". Classical Quarterly, ... 25 (5): 573-582. doi:10.1111/j.1574-6976.2001.tb00592.x. ISSN 1574-6976. PMID 11742692. Bapteste, Eric; Walsh, David A. (June ...

*Balo concentric sclerosis

Nov 2010). "Aquaporin-4 astrocytopathy in Baló's disease". Acta Neuropathol. 120 (5): 651-60. doi:10.1007/S00401-010-0733-7. ... Nevertheless, this model is questioned by recent reports that found astrocyte damage, similar to the one found in aquaporin- ... 29 (5): 603-7. doi:10.1177/0883073813517294. PMID 24423690. Lindquist S (2007). "Histopathology and serial, multimodal magnetic ... Neurology April 5, 2016 vol. 86 no. 16 Supplement I10.004 Genetic susceptibility in MS - Steve Hauser. Rare Neuroimmunologic ...

*List of MeSH codes (D12.776.157)

... aquaporin 3 MeSH D12.776.157.530.400.500.040.249.750 -- aquaporin 6 MeSH D12.776.157.530.400.500.040.374 -- aquaporin 1 MeSH ... aquaporin 4 MeSH D12.776.157.530.400.500.040.484 -- aquaporin 5 MeSH D12.776.157.530.400.500.520 -- voltage-dependent anion ... aquaporins MeSH D12.776.157.530.400.500.040.249 -- aquaglyceroporins MeSH D12.776.157.530.400.500.040.249.500 -- ... D12.776.157.530.400.500.040.437 -- aquaporin 2 MeSH D12.776.157.530.400.500.040.468 -- ...

*List of MeSH codes (D12.776.543)

... aquaporin 3 MeSH D12.776.543.550.425.730.040.249.750 -- aquaporin 6 MeSH D12.776.543.550.425.730.040.374 -- aquaporin 1 MeSH ... aquaporin 3 MeSH D12.776.543.585.400.730.040.249.750 -- aquaporin 6 MeSH D12.776.543.585.400.730.040.436 -- aquaporin 1 MeSH ... aquaporin 4 MeSH D12.776.543.550.425.730.040.484 -- aquaporin 5 MeSH D12.776.543.550.425.730.520 -- voltage-dependent anion ... aquaporin 4 MeSH D12.776.543.585.400.730.040.600 -- aquaporin 5 MeSH D12.776.543.585.400.730.520 -- voltage-dependent anion ...

*AQP9

Aquaporin-9 is a protein that in humans is encoded by the AQP9 gene. The aquaporins/major intrinsic protein are a family of ... Aquaporin 9 has greater sequence similarity with AQP3 and AQP7 and they may be a subfamily. Aquaporin 9 allows passage of a ... AQP9 aquaporin 9". Ishibashi K, Kuwahara M, Gu Y, et al. (1998). "Cloning and functional expression of a new aquaporin (AQP9) ... 2003). "Aquaporin-9 is expressed in a mucus-secreting goblet cell subset in the small intestine". FEBS Lett. 540 (1-3): 157-62 ...

*Glymphatic system

Two types of aquaporins are expressed in the CNS: aquaporin-1, which is expressed by specialized epithelial cells of the ... Aquaporins are membrane-bound channels that play critical roles in regulating the flux of water into and out of cells. Relative ... Aquaporin-4 expression in astrocytes is highly polarized to the endfoot processes ensheathing the cerebral vasculature. Up to ... Yool AJ (2007). "Aquaporins: multiple roles in the central nervous system". Neuroscientist. 13 (5): 470-85. doi:10.1177/ ...

*Major intrinsic proteins

The MIP superfamily includes three subfamilies: aquaporins, aquaglyceroporins and S-aquaporins. The aquaporins (AQPs) are water ... Aquaporin-1 (Aqp1) from the human red blood cell has been solved by electron crystallography to 3.8 Å resolution (PDB: 1FQY​). ... Aquaporins generally have the NPA motif in both halves, the glycerol facilitators generally have an NPA motif in the first ... Roles of aquaporins in human cancer have been reviewed as have their folding pathways. AQPs may act as transmembrane ...

*Daf-2

... elegans by downregulating daf-16/foxo activity and aquaporin gene expression". Cell Metabolism. 10 (5): 379-391. doi:10.1016/j. ... 5 (1): 31-7. doi:10.1111/j.1474-9726.2006.00188.x. PMC 1413578 . PMID 16441841. Dorman, Jennie B.; Albinder, Bella; Shroyer, ...

*SNAP23

... colocalization with aquaporin-2 in collecting duct vesicles". The American Journal of Physiology. 275 (5 Pt 2): F752-60. PMID ... 108 (3): 211-5. doi:10.1007/s004390100480. PMID 11354632. Shukla A, Corydon TJ, Nielsen S, Hoffmann HJ, Dahl R (Jul 2001). " ...

*STX4

... colocalization with aquaporin-2 in collecting duct vesicles". The American Journal of Physiology. 275 (5 Pt 2): F752-60. PMID ... possible role in aquaporin-2 trafficking". The Journal of Clinical Investigation. 98 (4): 906-13. doi:10.1172/JCI118873. PMC ... 3.0.CO;2-5. PMID 10508479. Paumet F, Le Mao J, Martin S, Galli T, David B, Blank U, Roa M (Jun 2000). "Soluble NSF attachment ... 74 (5): 863-73. doi:10.1016/0092-8674(93)90466-4. PMID 7690687. Li C, Ullrich B, Zhang JZ, Anderson RG, Brose N, Südhof TC (Jun ...

*AQP7

Aquaporin 7 and AQP3 are at the same chromosomal location suggesting that 9p13 may be a site of an aquaporin cluster. Aquaporin ... Aquaporin-7 is a protein that in humans is encoded by the AQP7 gene. Aquaporins/major intrinsic protein (MIP) are a family of ... "Entrez Gene: AQP7 aquaporin 7". Dibas AI, Mia AJ, Yorio T (1998). "Aquaporins (water channels): role in vasopressin-activated ... Aquaporin 7 has greater sequence similarity with AQP3 and AQP9 and they may be a subfamily. ...

*Vasopressin receptor 2

Robben JH, Knoers NV, Deen PM (Aug 2006). "Cell biological aspects of the vasopressin type-2 receptor and aquaporin 2 water ... Ishikawa SE (Feb 2002). "[Nephrogenic diabetes insipidus associated with mutations of vasopressin V2 receptors and aquaporin-2 ... 51 (5): 1078-83. PMC 1682836 . PMID 1415251. Birnbaumer M, Seibold A, Gilbert S, Ishido M, Barberis C, Antaramian A, Brabet P, ... 357 (6376): 333-5. doi:10.1038/357333a0. PMID 1534149. Lolait SJ, O'Carroll AM, McBride OW, Konig M, Morel A, Brownstein MJ ( ...

*Pediatric acquired brain injury

... been shown that more immature brains have an enlarged extracellular space volume and a decreased expression of glial aquaporin ... 40 (5): 572-579. doi:10.1097/00004583-200105000-00017. PMID 11349702. Yeates, KO (June 2005). "Long-term attention problems in ... 5, 6, 7. ,access-date= requires ,url= (help) Cole, Wesley; Paulos, Stephanie (2009). "A Review of Family Intervention ... Guidelines for Pediatric Acquired Brain Injuries": 2, 5, 6. ,access-date= requires ,url= (help) Gillett, Jane (2004). "The ...
AQP2 is found in the apical cell membranes of the kidneys collecting duct principal cells and in intracellular vesicles located throughout the cell. It is the only aquaporin regulated by vasopressin. The basic job of aquaporin 2 is to reabsorb water from the urine while its being removed from the blood by the kidney. Aquaporin 2 is in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed, vasopressin binds to the cell surface vasopressin receptor thereby activating a signaling pathway that causes the aquaporin 2 containing vesicles to fuse with the plasma membrane, so the aquaporin 2 can be used by the cell. This aquaporin is regulated in two ways by the peptide hormone vasopressin: short-term regulation (minutes) through trafficking of AQP2 vesicles to the apical region where they fuse with the apical plasma membrane long-term regulation (days) through an increase in AQP2 gene expression. This aquaporin is also regulated by food intake. Fasting ...
Plasma Membrane Abundance of Human Aquaporin 5 Is Dynamically Regulated by Multiple Pathways. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Gentaur molecular products has all kinds of products like :search , FabGennix \ Aquaporin 4, FITC conjugates \ AQP4-FITC for more molecular products just contact us
Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells. AQP4 is constitutively expressed. AQP4 is expressed in astrocytes and are upregulated by direct insult to the central nervous system. ...
Gentaur molecular products has all kinds of products like :search , FabGennix \ Aquaporin 8, Host species: Rabbit, Polyclonal antibody \ AQP-801AP for more molecular products just contact us
Aqp4 - Aqp4 (untagged) - Mouse aquaporin 4 (cDNA clone MGC:37514 IMAGE:4985265), (10ug) available for purchase from OriGene - Your Gene Company.
TY - JOUR. T1 - Expression and subcellular localization of aquaporin water channels in the polarized hepatocyte cell line, WIF-B. AU - Gradilone, Sergio A.. AU - Tietz, Pamela S.. AU - Splinter, Patrick L.. AU - Marinelli, Raúl A.. AU - LaRusso, Nicholas F.. PY - 2005/8/18. Y1 - 2005/8/18. N2 - Background: Recent data suggest that canalicular bile secretion involves selective expression and coordinated regulation of aquaporins (AQPs), a family of water channels proteins. In order to further characterize the role of AQPs in this process, an in vitro cell system with retained polarity and expression of AQPs and relevant solute transporters involved in bile formation is highly desirable. The WIF-B cell line is a highly differentiated and polarized rat hepatoma/human fibroblast hybrid, which forms abundant bile canalicular structures. This cell line has been reported to be a good in vitro model for studying hepatocyte polarity. Results: Using RT-PCR, immunoblotting and confocal immunofluorescence, ...
Aquaporin-5 is a protein that in humans is encoded by the AQP5 gene. Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. Aquaporin GRCh38: Ensembl release 89: ENSG00000161798 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000044217 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Lee MD, Bhakta KY, Raina S, Yonescu R, Griffin CA, Copeland NG, Gilbert DJ, Jenkins NA, Preston GM, Agre P (Jun 1996). "The human Aquaporin-5 gene. Molecular characterization and chromosomal localization". J Biol Chem. 271 (15): 8599-604. doi:10.1074/jbc.271.15.8599. PMID 8621489. "Entrez Gene: AQP5 aquaporin 5". Verkman AS (2003). "Role of aquaporin water channels in eye function". Exp. Eye Res. 76 (2): 137-43. ...
Aquaporins (AQPs) are membrane proteins that enable water transport across cellular plasma membranes in response to osmotic gradients. Phenotypic analyses have revealed important physiological roles for AQPs, and the potential for AQP water channel modulators in various disease states has been proposed. For example, AQP1 is overexpressed in tumor microvessels, and this correlates with higher metastatic potential and aggressiveness of the malignancy. Chemical modulators would help in identifying the precise contribution of water channel activity in these disease states. These inhibitors would also be important therapeutically, e.g., in anti-cancer treatment. This perceived importance contrasts with the lack of success of high-throughput screens (HTS) to identify effective and specific inhibitors of aquaporins. In this paper, we have screened a library of 1500
Aquaporin 1: Aquaporin 1 forms a water-specific channel that is constitutively expressed at the PLASMA MEMBRANE of ERYTHROCYTES and KIDNEY TUBULES, PROXIMAL. It provides these cells with a high permeability to WATER. In humans polymorphisms of this protein result in the Colton blood group antigen.
Aquaporin A/S is a global cleantech company located in Kongens Lyngby, Denmark. Aquaporin is dedicated to revolutionizing water purification through the use of industrial biotechn
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
collections, groups of modules structured into books or course notes, or for other uses. Our open license allows for free use and reuse of all our content. ...
Marinelli RA, Pham L, Agre P, La Russo NF. Secretin promotes osmotic water transport in rat cholangiocytes by incresing aquaporin-1 water channels in plasma membrane. Evidence for a secretin-induced vesicular translocation of aquaporin-1. J Biol Chem 1997; 272: 12984-12988 ...
Aquaporin membrane protein, molecular model. Computer illustration showing the structure of a molecule of the human aquaporin 1 protein (blue, and white ribbon). Aquaporins are membrane proteins that form channels (centre) that help water molecules (red and white spheres) pass in and out of cells. Unlike ion channels, aquaporins help prevent ions and other dissolved substances carrying electrical charge from entering the cell, as they only allow lone water molecules or certain uncharged solutes to pass through. This helps maintain the electrochemical potential of the cell membrane. - Stock Image C035/5236
After producing a recombinant form of AqpZ in E. coli, the proteins were crystallized--capturing five water molecules inside--and then analyzed by state-of-the-art high-resolution X-ray diffraction techniques. The architecture of aquaporin Z is typical of aquaporins, with a spiral of eight oxygens providing water-binding sites inside the channel. The outer membrane and cytoplasmic ends of the channel are wider than the interior, which is long and narrow. This structure demonstrates that aquaporin selectivity arises in part from erecting a physical barrier: small molecules, like water, can easily pass, but larger ones simply cant fit. And the strategic positioning of amino acid residues with hydrophilic or hydrophobic properties along the channel helps police the influx of molecules based on their affinity for water. While it seems two amino acid chains located in the middle of the channel also provide a water-friendly surface, Stroud et al. say they play a more intriguing role. Noting that the ...
Polyclonal antibody for AQUAPORIN 3/AQP3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. AQUAPORIN 3/AQP3 information: Molecular Weight: 31544 MW; Subcellular Localization: Basolateral cell membrane; Multi-pa
Aquaporin 10 antibody, C-term (aquaporin 10) for WB. Anti-Aquaporin 10 pAb (GTX45889) is tested in Human samples. 100% Ab-Assurance.
Purpose: The expression of aquaporin water channel genes are shown to be affected in several pathological conditions of retina, such as in diabetic retinopathy, retinal ischemia and in autoimmune uveitis. Human native retinal pigment epithelial (RPE) cells and immortalized human RPEs are formerly shown to express aquaporins, still the expression of aquaporins in stem cell derived RPE have not been previously elucidated. The objective of this study was to determine the expression of several aquaporin genes (aquaporin1,- 3, -4, -5, -6, -7, -10, -11 and -12) and assess the localization of aquaporin 1 water channel protein in human embryonic (hESCs) and induced pluripotent stem cells (hiPSCs) derived RPE cells.. Methods: hESC- and hiPSC derived RPE cells were grown as monolayer in serum-free media. The expression of aquaporin genes was determined with qRT-PCR. The localization of AQP1-protein was studied with confocal microscopy. Finally, the functionality of aquaporins was assessed with dye ...
With Aquaporin Inside™ Reverse Osmosis membranes, water treatment can be done with lower energy consumption compared to conventional membrane technology. The benefit comes from the aquaporin proteins which are very efficient in transporting water. This enables the water treatment plant to increase the capacity of treated wastewater without increasing the energy consumption, or remaining at the same capacity level while lowering the energy consumption. At the same time, Aquaporin membranes has the potential to remove also small, neutral compounds such as micro pollutants/trace organics, improving re-use water quality.. Aquaporin Inside™ Forward Osmosis ...
Looking for online definition of Aquaporin1 or what Aquaporin1 stands for? Aquaporin1 is listed in the Worlds largest and most authoritative dictionary database of abbreviations and acronyms
Background Aquaporins (Aqps) are integral membrane proteins that facilitate the transport of water and small solutes across cell membranes. Among vertebrate species, Aqps are highly conserved in both gene structure and amino acid sequence. These proteins are vital for maintaining water homeostasis in living organisms, especially for aquatic animals such as teleost fish. Studies on teleost Aqps are mainly limited to several model species with diploid genomes. Common carp, which has a tetraploidized genome, is one of the most common aquaculture species being adapted to a wide range of aquatic environments. The complete common carp genome has recently been released, providing us the possibility for gene evolution of aqp gene family after whole genome duplication. Results In this study, we identified a total of 37 aqp genes from common carp genome. Phylogenetic analysis revealed that most of aqps are highly conserved. Comparative analysis was performed across five typical vertebrate genomes. We found that
Müller glial cells are important regulators of physiological function of retina. In a model disease of retinal inflammation and spontaneous recurrent uveitis in horses (ERU), we could show that retinal Müller glial cells significantly change potassium and water channel protein expression during autoimmune pathogenesis. The most significantly changed channel protein in neuroinflammatory ERU was aquaporin 11 (AQP11). Aquaporins (AQP, 13 members) are important regulators of water and small solute transport through membranes. AQP11 is an unorthodox member of this family and was assigned to a third group of AQPs because of its difference in amino acid sequence (conserved sequence is only 11 %) and especially its largely unknown function. In order to gain insight into the distribution, localization, and function of AQP11 in the retina, we first developed a novel monoclonal antibody for AQP11 enabling quantification, localization, and functional studies. In the horse retina, AQP11 was exclusively expressed
Purpose: : To demonstrate in the conjunctiva the presence of aquaporin type 5 (AQP5), a water channel homologue found in the apical membrane of several tissues including the cornea. Presently, there are no reports indicating as to which AQP might be expressed apically in the conjunctiva; only AQP3 has been identified in the lateral membranes of rat and human conjunctival epithelia. Because we had data from gene-expression microarray assays (Turner; ARVO 2004) indicating message for AQP5 in the human conjunctiva, tissue samples from human, as well as from rats and rabbits (given the ease of their procurement), were analyzed to confirm that the AQP5 protein was indeed expressed in mammalian conjunctivae. Methods: : Goat polyclonal IgG against AQP5 was purchased commercially and used in immunoblotting and immunohistochemical techniques to identify and localize the water channel in rat, rabbit and human epithelia. Results: : Immunoblot analysis of rabbit bulbar-plus-palpebral plasma membrane ...
Cevimeline is a parasympathomimetic and muscarinic agonist, with particular effect on M3 receptors. It is indicated by the Food and Drug Administration for the treatment of dry mouth associated with Sjögrens syndrome.
This paper strongly supports the conclusion that AQP0 has a high permeability mode and a low permeability mode. How do these two modes differ? Available structural data on the nature of the water-filled pore through AQP1 and the glycerol facilitator suggest a possible answer. For both, water and glycerol must move through the pore by single-file diffusion. The crystal structure and molecular dynamic simulations suggest that there are multiple water molecules in the pore. There are ∼6 waters seen in the narrow constriction of the pore in the X-ray structure (Sui et al., 2001), and molecular dynamic simulations suggest that there are ∼7 or 8 water molecules moving in concert in the single-file portion of the pore (Tajkhorshid et al., 2002; Zhu et al., 2004). The lack of passage of ionic current through AQP0 and AQP1 is explained by electrostatic considerations that strongly inhibit the movement of protons into the NPA region or hydroxyls into regions flanking either side of the NPA region (de ...
Aquaporin (AQP) proteins comprise a group of membrane intrinsic proteins (MIPs) that are responsible for transporting water and other small molecules, which is crucial for plant survival under stress conditions including salt stress. Despite the vital role of AQPs, little is known about them in cucumber (Cucumis sativus L.). In this study, we identified 39 aquaporin-encoding genes in cucumber that were separated by phylogenetic analysis into five sub-families (PIP, TIP, NIP, SIP, and XIP). Their substrate specificity was then assessed based on key amino acid residues such as the aromatic/Arginine (ar/R) selectivity filter, Frogers positions, and specificity-determining positions. The putative cis-regulatory motifs available in the promoter region of each AQP gene were analyzed and results revealed that their promoter regions contain many abiotic related cis-regulatory elements. Furthermore, analysis of previously released RNA-seq data revealed tissue- and treatment-specific expression patterns of
Aquaporin 8 (AQP8) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 8 mRNA is found in pancreas and colon but not other tissues. [provided by RefSeq, Jul 2008 ...
Bronchopulmonary dysplasia (BPD) remains a major respiratory illness in extremely premature infants. The biological mechanisms leading to BPD are not fully understood, although an arrest in lung development has been implicated. The current study aimed to investigate the occurrence of autophagy in the developing mouse lung and its regulatory role in airway branching and terminal sacculi formation. We found 2 windows of epithelial autophagy activation in the developing mouse lung, both resulting from AMPK activation. Inhibition of AMPK-mediated autophagy led to reduced lung branching in vitro. Conditional deletion of beclin 1 (Becn1) in mouse lung epithelial cells (Becn1Epi-KO), either at early (E10.5) or late (E16.5) gestation, resulted in lethal respiratory distress at birth or shortly after. E10.5 Becn1Epi-KO lungs displayed reduced airway branching and sacculi formation accompanied by impaired vascularization, excessive epithelial cell death, reduced mesenchymal thinning of the interstitial ...
Bronchopulmonary dysplasia (BPD) remains a major respiratory illness in extremely premature infants. The biological mechanisms leading to BPD are not fully understood, although an arrest in lung development has been implicated. The current study aimed to investigate the occurrence of autophagy in the developing mouse lung and its regulatory role in airway branching and terminal sacculi formation. We found 2 windows of epithelial autophagy activation in the developing mouse lung, both resulting from AMPK activation. Inhibition of AMPK-mediated autophagy led to reduced lung branching in vitro. Conditional deletion of beclin 1 (Becn1) in mouse lung epithelial cells (Becn1Epi-KO), either at early (E10.5) or late (E16.5) gestation, resulted in lethal respiratory distress at birth or shortly after. E10.5 Becn1Epi-KO lungs displayed reduced airway branching and sacculi formation accompanied by impaired vascularization, excessive epithelial cell death, reduced mesenchymal thinning of the interstitial ...
Objective: It is well known that VEGFR expression is correlated with peritumoral brain edema in meningiomas. Also Aquaporin4 seems to induce the perifocal edema. In our study we analyzed the correlation of VEGFR1, VEGFR2 and Aquaporin4 to the brain edema. Additionally a potential correlation between[for full text, please go to the a.m. URL ...
Hi, Bit more than a month ago my mom developed burning sensation on her back and stomach, she went to a doc but he said its nothing lifetreatening and she waited for for her turn for the tests, it was like this for a week or two. Few weeks ago the burning sensation became too much to bear and my mom went to a hospital. They took her in and the next day she had issues walking and day later she couldnt walk on her own at all. After a few days they put her on some steroid IV thing for
Hi, Bit more than a month ago my mom developed burning sensation on her back and stomach, she went to a doc but he said its nothing lifetreatening and she waited for for her turn for the tests, it was like this for a week or two. Few weeks ago the burning sensation became too much to bear and my mom went to a hospital. They took her in and the next day she had issues walking and day later she couldnt walk on her own at all. After a few days they put her on some steroid IV thing for
Immunohistochemistry: STK39 Antibody [NBP2-49347] - Staining of human salivary gland shows strong cytoplasmic and nuclear positivity in glandular cells ...
Xerostomia is estimated to affect 30% of adults over the age of 65. The condition is characterised by a lack of saliva secretion, resulting in a range of health problems. A rise in the concentration of free cytosolic calcium (Ca²⁺) is essential to initiate saliva secretion. We construct mathematical models of saliva secretion and Ca²⁺ dynamics in salivary acinar and duct cells. We investigate how the distribution of K⁺ channels affects the rate of primary saliva secretion. Maximum saliva secretion is hypothesised to occur when a small amount of K⁺ conductance is located in the apical membrane, with the majority in the basolateral membrane. Apical K⁺ channels have since been experimentally located. For a range of applied agonist, the concentration of Ca²⁺ in salivary cells is seen experimentally to oscillate and to travel in waves across the cytosol. We construct a model of Ca2+ oscillations in parotid acinar cells that requires paired oscillations of IP₃. This ODE model ...
... (AQP4) inhibitor 2-(nicotinamide)-1,3,4-thiadiazole, TGN-020, inside a mouse style of focal cerebral ischemia using 7. research convincingly exhibited that pretreatment using the AQP4 inhibitor TGN-020 considerably reduced the quantity of mind edema connected with ischemic damage. Ischemic edema is usually thought to be initiated by influx of Na+ connected with energy failing. Higher osmolarity circumstances create the generating force for drinking water influx into cells, leading to ionic edema [17]. This early edema stage, so-called cytotoxic edema, is certainly thought to last a long time before mass leakage of drinking water into the human brain ensues, making so-called vasogenic edema [18]. AQP4 is certainly thought to play a substantial function in the real drinking water flux in both procedures. Flux through AQP4 is certainly buy 154992-24-2 bidirectional and solely reliant on osmolarity distinctions between your two spaces ...
TY - JOUR. T1 - Involvement of aquaporin in thromboxane A2 receptor-mediated, G12/13/RhoA/NHE-sensitive cell swelling in 1321N1 human astrocytoma cells. AU - Saito, Masaki. AU - Tanaka, Hiroyuki. AU - Sasaki, Masako. AU - Kurose, Hitoshi. AU - Nakahata, Norimichi. PY - 2010/1/1. Y1 - 2010/1/1. N2 - The physiological role of the thromboxane A2 (TXA2) receptor expressed on glial cells remains unclear. We previously reported that 1321N1 human astrocytoma cells pretreated with dibutyryl cyclic AMP (dbcAMP) became swollen in response to U46619, a TXA2 analogue. In the present study, we examined the detailed mechanisms of TXA2 receptor-mediated cell swelling in 1321N1 cells. The cell swelling caused by U46619 was suppressed by expression of p115-RGS, an inhibitory peptide of Gα12/13 pathway and C3 toxin, an inhibitory protein for RhoA. The swelling was also inhibited by treatment with Y27632, a Rho kinase inhibitor and 5-(ethyl-N-isopropyl)amiloride (EIPA), a Na+/H+-exchanger inhibitor. Furthermore, ...
It is well-known that the rapid now of water into and out of cells is controlled by membrane proteins called aquaporins (AQPs). However, the mechanisms that allow cells to quickly respond to a changing osmotic environment are less well established. Using GFP-AQP fusion proteins expressed in HEK293 cells, we demonstrate the reversible manipulation of cellular trafficking of AQP1. AQP1 trafficking was mediated by the tonicity of the cell environment in a specific PKC- and microtubule-dependent manner. This suggests that the increased level of water transport following osmotic change may be due it phosphorylation-dependent increase in the level of AQP1 trafficking resulting in membrane localization.. ...
Affiliation:Nagoya University of Arts and Sciences,栄養学部,教授, Research Field:Gastroenterology,Gastroenterology,General physiology, Keywords:CFTR,単離小葉間膵管,膵導管細胞,5-hydroxytriptamine,水チャネル,HCO_3^-分泌,aquaporin,Na^+-HCO_3^-共輸送体,pancreatic duct cell,細胞内Cl^-濃度, # of Research Projects:9, # of Research Products:0
Plant cells contain proteins that are members of the major intrinsic protein (MIP) family, an ancient family of membrane channel proteins characterized by six membrane-spanning domains and two asparagine-proline-alanine (NPA) amino acid motifs in the two halves of the protein. We recently demonstrated that [gamma]-TIP, one of the MIP homologs found in the vacuolar membrane of plant cells, is an aquaporin or water channel protein (C. Maurel, J. Reizer, J.I. Schroeder, M.J. Chrispeels [1993] EMBO J 12: 2241-2247). RD28, another MIP homolog in Arabidopsis thaliana, was first identified as being encoded by a turgor-responsive transcript. To find out if RD28 is a water channel protein, rd28 cRNA was injected into Xenopus laevis oocytes. Expression of RD28 caused a 10- to 15-fold increase in the osmotic water permeability of the oocytes, indicating that the protein creates water channels in the plasma membrane of the oocytes and is an aquaporin just like its homolog [gamma]-TIP. Although RD28 has ...
Water deficit (WD) is a growing problem in agriculture. In citrus crops, genetically-determined characteristics of the rootstock are important factors in plant responses to WD. Aquaporins are involved in regulating the water supply to the plant by mediating water flow through the cell membranes. Recent studies support a direct role for aquaporins in plant water relations and demonstrate their involvement in tolerance to WD. This study investigates the relationship between photosynthetic and water-balance parameters with levels of expression of aquaporins in conditions of moderate WD in the rootstocks Poncirus trifoliata (L.) Raf. (PT), Cleopatra Mandarin (Citrus reshni Hort. ex Tan.) (CM) and 030115 (a hybrid of the two former rootstocks). Under conditions of WD, the hybrid 030115 drastically reduced aquaporin expression, accompanied by a loss of plant vigour but without reducing the net CO2 assimilation (ACO2). PT maintained the same level of aquaporin expression under WD as under normal ...
In plant sexual reproduction, water and solute movement are tightly regulated, suggesting the involvement of aquaporins. We previously identified TIP5;1 and TIP1;3 as the only Arabidopsis aquaporin genes that are selectively and highly expressed in mature pollen, and showed that they can transport both water and urea when expressed in Xenopus oocytes. Here, we show that TIP5;1 has unusual characteristics, as its water transport activity is regulated by pH. Analysis of the water transport activity of a mutant version of TIP5;1 (TIP5;1-H131A) and amino acid alignment with other plant aquaporins regulated by pH suggested that a conserved motif is involved in pH sensing. GFP-TIP5;1 is located in the mitochondria of pollen tubes. The single mutants tip1;3 and tip5;1, as well as the tip1;3 tip5;1 double mutant, are fertile, but all mutants had shorter than normal pollen tubes when germinated in vitro in the absence of exogenous nitrogen. Thus, we propose that TIP5;1 and TIP1;3 are involved in nitrogen ...
Structural data on AQPs, together with mutagenesis and molecular dynamics simulations, have indicated that single-file transport occurs through a narrow pore in each monomer, where water selectivity is conferred by electrostatic and steric factors (Hub et al., 2009; Khalali-Araghi et al., 2009). Glycerol-transporting AQPs, called aquaglyceroporins, have a less-constricted pore compared with that of water-selective AQPs (diameter of 3.4 Å compared with 2.8 Å, respectively), with relatively more hydrophobic residues lining the pore.. Many mammalian AQPs, including AQP1, AQP2, AQP4, AQP5 and AQP8, function primarily as bidirectional water-selective transporters. Cells expressing AQPs on their plasma membrane have an ~5- to 50-fold higher osmotic water permeability than membranes that do not (Verkman and Mitra, 2000). Water transport through single-file pores poses a biophysical limitation on the efficiency with which AQPs can transport water, so that AQPs must be present in the membrane at a high ...
Peter Agre discovered the first aquaporin in 1992 in red blood cells and was awarded the 2003 Nobel Prize. Since then, 13 variants of aquaporin have been found in animals and humans and 35 in plants. There are thousands of these aquaporins in every cell membrane. Aquaporins contain a conduit that is so tiny that only a single water molecule at a time can pass through it. But this traffic can be lively indeed. In one second, several billion water molecules can get through. The direction of this water flow is contingent on the osmotic pressure. The water moves in a direction away from a low and toward a high concentration of salt and nutritional substances. But the conduit isnt always open. The Lund scientists have found out how it opens and closes. This was done in collaboration with a team at Chalmers University of Technology in Göteborg, Sweden, under the direction of Richard Neutze, and with Emad Tajkhorshid at the University of Illinois ...
Sigma-Aldrich offers abstracts and full-text articles by [Xiao-Qiang Liu, Hideyuki Kobayashi, Zi-Bing Jin, Akihiko Wada, Nobuhis Nao-I].
Anti-Aquaporin 3 Antibody (#AQP-003) from Alomone Labs is a highly specific rabbit polyclonal Ab directed against an epitope of rat AQP3. Applications: IFC, IHC, IP, WB. Free samples available. Control antigen included. Lyophilized. Global shipping at room temperature. Your top supplier for aquaporin research!
Affiliation:University of Miyazaki,Faculty of Medicine,Assistant, Research Field:Otorhinolaryngology, Keywords:Aquaporin,生体分子,Dexamethasone,lippopolysaccaride,Immunohistocemistru,免疫染色法,嗅覚,パッチクランプ,難聴,モータ蛋白, # of Research Projects:3, # of Research Products:39
From guiding branching neurons in the developing brain to maintaining a healthy heartbeat, there seems to be no job that the immune cells cant tackle.. 0 Comments. ...
In Chapter 6, "The Hormonal Essence of the T-Rex?" author Cordelia Fine considers the biological dogma that testes, and the powerful hormones they exude, are the root of all sexual inequality.. 0 Comments. ...
Background: The purpose of the present study was to elucidate whether myoepithelial cells proliferate mitotically during regeneration of rat submandibular glands after atrophy.. Methods: The excretory duct of the right submandibular gland of rats was doubly ligated near the hilum with metal clips, which were removed after 7 days of ligation (day 0). The regenerating right submandibular glands were removed from 0 to 14 days after removal of the clips. The removed tissue was examined with immunohistochemical double staining for proliferating cell nuclear antigen (PCNA) as a marker of proliferating cells and actin as a marker of myoepithelial cells, as well as with transmission electron microscopy (TEM).. Results: The PCNA-positive myoepithelial cells were observed at the periphery of transitional duct-acinar structures, ducts and acini in the regenerating glands at every time-point, and the PCNA-labeling index of myoepithelial cells increased greatly especially between day 2 and 4. The mitosis of ...
Effect of Parotid Saliva Secretion on Dry Forage Intake in Goats - Parotid Saliva Secretion;Thirst Sensation;Dry Forage Intake;Goats;
Vasopressin is the key regulator of water homeostasis in vertebrates. Central to its antidiuretic action in mammals is the redistribution of the water channel aquaporin 2 (AQP2) from intracellular vesicles to the apical membrane of kidney epithelial cells, an event initiated by an increase in cAMP and activation of protein kinase A. The subsequent steps of the signaling cascade are not known. To identify proteins involved in the AQP2 shuttle we exploited a recently developed cell line (CD8) derived from the rabbit cortical collecting duct and stably transfected with rat AQP2 cDNA. Treatment of CD8 cells with pertussis toxin (PTX) inhibited both the vasopressin-induced increase in water permeability and the redistribution of AQP2 from an intracellular compartment to the apical membrane. ADP-ribosylation studies revealed the presence of at least two major PTX substrates. Correspondingly, two alpha subunits of PTX-sensitive G proteins, Galphai2 and Galphai3, were identified by Western blotting.
plasma membrane intrinsic protein 2-7, PIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, P93004, Q9ATM4 Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane
.Objectives: To measure phosphorus metabolites in human parotid glands by 31P-MRS using three-dimensional chemical-shift imaging (3D-CSI), and ascertain whether this method can capture changes in adenosine triphosphate (ATP) and phosphocreatine (PCr) levels due to saliva secretion. Study Design: The parotid glands of 20 volunteers were assessed by 31P-MRS using 3D-CSI on 3T MRI. After obtaining a first (baseline) measurement, the participants took vitamin-C tablets and measurements were obtained twice more, in a continuous manner. The peak area ratios of PCr and β-ATP were evaluated. Results: A high proportion of PCr (0.26 ± 0.08) and ATP (α: 0.16 ± 0.06; β: 0.27 ± 0.06; γ: 0.21 ± 0.03) was noted at baseline. A significant decrease in β-ATP was observed between baseline (
The ability of eukaryotic cells to change their shape and to migrate directionally is highly dependent on active volume regulation in cells building up tissues as well as in individual cells. Transmembrane fluxes of water via specialized water channels, called aquaporins (AQPs), facilitate the changes of volume and shape, which additionally require a complex interplay between the plasma membrane and the cytoskeleton. AQPs have been shown to be involved in the development of inflammatory processes and diseases. The aims of the studies underlying this thesis were to further elucidate the expression and function of AQPs in both bacterial and viral infections as well as in the inflammatory disease, microscopic colitis. For this, molecular techniques qPCR, immunoblotting and live, holographic, confocal and super-resolution imaging were used.. When cells of the innate immune system encounter pathogens they need to respond and prepare for migration and phagocytosis and do so through volume regulatory ...
The figure at the left displays the protein interaction network for human AQP2(red dot in center) as generated using STRING (4). Below is a pie chart depicting the biological processes that the proteins identified in this network are involved in. The ontologies of these interactors were visualized using PANTHER (5). The most dominant functional categories of the interacting proteins are involvement in cellular processes(including signal transduction), localization(including transport), multicellular organism processes, and biological regulation. One result of note is the inclusion of several other aquaporins within this network, including AQP1, AQP3, and AQP4. This opens the possibility for some overlap in coregulation of these different aquaporins ...
Order monoclonal and polyclonal Aquaporin 5 antibodies for many applications. Selected quality suppliers for anti-Aquaporin 5 antibodies.
Rabbit polyclonal Aquaporin 4 antibody validated for WB, ICC/IF and tested in Human, Mouse and Rat. Immunogen corresponding to synthetic peptide
Rabbit polyclonal Aquaporin 0 antibody validated for WB and tested in Human, Mouse and Rat. Immunogen corresponding to synthetic peptide
Please Note, The ebooks are not always PDF format, you might receive epub/kindle formats after purchase. This is Digital Version of (Ebook) 978-3
In this study, we analyzed defined epitopes in the extracellular loops of AQP4 recognized by AQP4-IgG positive serum and CSF samples of 47 NMOSD patients. Overall, we found reduced binding to all mutations and AQP4-M1 compared to the AQP4-M23 isoform and distinguished two broad patterns of AQP4-IgG recognition, a loop A-dependent pattern A and a loop A-independent pattern B. Further, we observed reduced binding to multiple AQP4 mutants in the majority of all NMOSD patients indicating that the human AQP4 antibody response is defined by multiple epitopes. These findings are consistent with previous studies showing that serum autoantibodies bind to multiple targets including AQP4 peptides, monomers, and higher order arrays [15, 34]. A comparable study on conformational epitopes of the myelin oligodendrocyte glycoprotein (MOG) could show that the immune response can be directed against one single or multiple epitopes [33].. Introduction of a myc-tag in the extracellular loops A, C, and E reduced the ...
Significant progress in the understanding of imaging conditions and the interpretation of topographs recorded with the AFM has allowed the surface topography of bacteriorhodopsin to be correlated with the helixconnecting loops to a lateral resolution of 5 Å (Müller et al., 1999b). Here we have used this technology to study the surface of AqpZ, the first bacterial water channel identified (Calamita et al., 1995). Its overexpression, isolation and 2D crystallization have recently been described (Borgnia et al., 1999; Ringler et al., 1999).. 2D crystals adsorbed firmly and without folds or wrinkles to freshly cleaved mica in a high ionic strength buffer (Müller et al., 1997). Subsequent change to a buffer adjusted to compensate for van der Waals interactions allowed their height to be measured accurately (Müller and Engel, 1997). The result, 57 ± 4 Å, compares favorably with the height previously reported for AQP1, 58 ± 3 Å (Walz et al., 1996).. The p4212 crystals of AqpZ with unit cell ...
Using computer simulations and experimental results, researchers at the University of Illinois at Urbana-Champaign and the University of Arizona have identified a key component of the gating mechanism in aquaporins that controls ...

Fatal anti-aquaporin-4 seropositive neuromyelitis optica spectrum disorder in tuberculosis | BMC Infectious Diseases | Full TextFatal anti-aquaporin-4 seropositive neuromyelitis optica spectrum disorder in tuberculosis | BMC Infectious Diseases | Full Text

This is the first reported case of anti-aquaporin-4 antibody-positive NMO spectrum disorder in a patient with active ... It shows the usefulness of testing for anti-aquaporin-4 antibodies while evaluating neurological deterioration in patients with ... Spinal imaging and anti-aquaporin-4 antibody positivity established a diagnosis of neuromyelitis optica spectrum disorder. ... We report a fatal case of anti-aquaporin-4 antibody positive NMO spectrum disorder in a patient who was receiving treatment for ...
more infohttps://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-14-470

Anti-Aquaporin 5 antibody (ab15119) | AbcamAnti-Aquaporin 5 antibody (ab15119) | Abcam

Rabbit polyclonal Aquaporin 5 antibody. Validated in WB, ELISA and tested in Rat. Independently reviewed in 1 review(s). ... Primary - Rabbit Anti-Aquaporin 5 antibody (ab15119) WB, ELISA Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718) IHC-P, WB ... Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the ... Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 5 antibody. Average ...
more infohttps://www.abcam.com/aquaporin-5-antibody-ab15119.html

Product Overview anti-Aquaporin 5 AntibodiesProduct Overview anti-Aquaporin 5 Antibodies

Order monoclonal and polyclonal Aquaporin 5 antibodies for many applications. Selected quality suppliers for anti-Aquaporin 5 ... Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 ... Top anti-Aquaporin 5 Antibodies at antibodies-online.com. Showing 10 out of 83 products:. Catalog No.. Reactivity. Host. ... Top referenced anti-Aquaporin 5 Antibodies. Show all anti-Aquaporin 5 (AQP5) Antibodies with Pubmed References. * Human ...
more infohttps://www.antibodies-online.com/abstract/Aquaporin+5+

Anti-Aquaporin 5 Rabbit pAb | 178615Anti-Aquaporin 5 Rabbit pAb | 178615

Anti-Aquaporin 5 Rabbit pAb Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue ... Anti-Aquaporin 5 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available ... Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ... More,, Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated ...
more infohttp://www.emdmillipore.com/PR/en/product/Anti-Aquaporin-5-Rabbit-pAb,EMD_BIO-178615

Stimulation of aquaporin-5 and transepithelial water permeability in human airway epithelium by hyperosmotic stress |...Stimulation of aquaporin-5 and transepithelial water permeability in human airway epithelium by hyperosmotic stress |...

Hwang SM, Lee RH, Song JM, Yoon S, Kim YS, Lee SJ, Kang SK, Jung JS (2002) Expression of aquaporin-5 and its regulation in ... Krane CM, Fortner CN, Hand AR, McGraw DW, Lorenz JN, Wert SE, Towne JE, Paul RJ, Whitsett JA, Menon AG (2001) Aquaporin 5- ... Leitch V, Agre P, King LS (2001) Altered ubiquitination and stability of aquaporin-1 in hypertonic stress. Proc Natl Acad Sci U ... Verkman AS, Matthay MA, Song Y (2000) Aquaporin water channels and lung physiology. Am J Physiol Lung Cell Mol Physiol 278(5): ...
more infohttps://link.springer.com/article/10.1007/s00424-006-0157-3

AQP5 / Aquaporin 5 Antibody (C-Terminus) for ELISA, IF/Immunofluorescence,AQP5 / Aquaporin 5 Antibody (C-Terminus) for ELISA, IF/Immunofluorescence,

Aquaporin 5 antibody LS-C3809 is an unconjugated rabbit polyclonal antibody to Aquaporin 5 (AQP5) from human, mouse and rat. ... Aquaporin 5 antibody LS-C3809 is an unconjugated rabbit polyclonal antibody to Aquaporin 5 (AQP5) from human, mouse and rat. ... Aquaporin 5 antibody LS-C3809 is an unconjugated rabbit polyclonal antibody to Aquaporin 5 (AQP5) from human, mouse and rat. ... Recognizes rat Aquaporin 5 (AQP5). No significant homology is seen with other AQPs or any other protein. Species sequence ...
more infohttps://www.lsbio.com/antibodies/aqp5-antibody-aquaporin-5-antibody-c-terminus-elisa-if-immunofluorescence-ihc-wb-western-ls-c3809/4550

Functional requirement of aquaporin-5 in plasma membranes of sweat glandsFunctional requirement of aquaporin-5 in plasma membranes of sweat glands

The distribution and function of aquaporins (AQPs) have not previously been defined in sweat glands. In this study, AQP1, AQP3 ...
more infohttps://jscholarship.library.jhu.edu/handle/1774.2/33962

Aquaporin 5 promotes tumor migration and angiogenesis in non‑small cell lung cancer cell line H1299Aquaporin 5 promotes tumor migration and angiogenesis in non‑small cell lung cancer cell line H1299

... *Authors: *Abdalkhalig ... Aquaporin 5 (AQP5) may be involved in NSCLC by promoting lung‑cancer initiation and progression. The present study aimed to ... Elkhider, A., Wang, B., Ouyang, X., Al‑Azab, M., Walana, W., Sun, X., Li, H., Tang, Y., Wei, J., Li, X.Aquaporin 5 promotes ... Elkhider, A., Wang, B., Ouyang, X., Al‑Azab, M., Walana, W., Sun, X., Li, H., Tang, Y., Wei, J., Li, X.Aquaporin 5 promotes ...
more infohttps://www.spandidos-publications.com/10.3892/ol.2020.11251

Aquaporin 5 -1364A/C Promoter Polymorphism Is Associated with Pulmonary Inflammation and Survival in Acute Respiratory Distress...Aquaporin 5 -1364A/C Promoter Polymorphism Is Associated with Pulmonary Inflammation and Survival in Acute Respiratory Distress...

Polymorphisms of aquaporin-5, a key mediator of inflammation, may impact outcome.What This Article Tells Us That Is NewIn acute ... Polymorphisms of aquaporin-5, a key mediator of inflammation, may impact outcome.What This Article Tells Us That Is NewIn acute ... Aquaporin 5 -1364A/C Promoter Polymorphism Is Associated with Pulmonary Inflammation and Survival in Acute Respiratory Distress ... the C-allele of the aquaporin-5 -1364A/C promoter polymorphism is associated with less pulmonary inflammation and greater ...
more infohttps://medworm.com/668432693/aquaporin-5-1364ac-promoter-polymorphism-is-associated-with-pulmonary-inflammation-and-survival-/

AQP5 / Aquaporin 5 Antibody (C-Terminus) for ELISA, WB/Western LS-C3810AQP5 / Aquaporin 5 Antibody (C-Terminus) for ELISA, WB/Western LS-C3810

Aquaporin 5 antibody LS-C3810 is an unconjugated rabbit polyclonal antibody to Aquaporin 5 (AQP5) from mouse and rat. Validated ... Aquaporin 5 antibody LS-C3810 is an unconjugated rabbit polyclonal antibody to Aquaporin 5 (AQP5) from mouse and rat. Validated ... Aquaporin 5 antibody LS-C3810 is an unconjugated rabbit polyclonal antibody to Aquaporin 5 (AQP5) from mouse and rat. Validated ... Recognizes rat Aquaporin 5 (AQP5). No significant homology is seen with other AQPs or any other protein. Species sequence ...
more infohttps://www.lsbio.com/antibodies/aqp5-antibody-aquaporin-5-antibody-c-terminus-elisa-wb-western-ls-c3810/4551

The Muscarinic Acetylcholine Receptor-Stimulated Increase in Aquaporin-5 Levels in the Apical Plasma Membrane in Rat Parotid...The Muscarinic Acetylcholine Receptor-Stimulated Increase in Aquaporin-5 Levels in the Apical Plasma Membrane in Rat Parotid...

aquaporin. ACh. acetylcholine. mAChR. muscarinic acetylcholine receptor. APM. apical plasma membrane. SNI-2011. cevimeline ... 1999) Defective secretion of saliva in transgenic mice lacking aquaporin-5 water channels. J Biol Chem 274:20071-20074. ... Several aquaporins (AQPs), which form water channels that selectively transport water across the plasma membrane, have been ... 2000) Persistent increase in the amount of aquaporin-5 in the apical plasma membrane of rat parotid acinar cells induced by a ...
more infohttp://molpharm.aspetjournals.org/content/61/6/1423

KAKEN - Research Projects | Physiological roles ofpost-translational modifications of water channel aquaporin-5 in the salivary...KAKEN - Research Projects | Physiological roles ofpost-translational modifications of water channel aquaporin-5 in the salivary...

Journal Article] Novel phosphorylation of aquaporin-5 at its threonine 259 through cAMP signaling in salivary gland cells.2011 ... Physiological roles ofpost-translational modifications of water channel aquaporin-5 in the salivary glands.. Research Project ... Two types of post-translational modifications, phosphorylation and short-chain ubiquitination, of water channel aquaporin-5 ( ... Presentation] 唾液腺細胞におけるアクアポリン 5 の翻訳後修2011. *. Author(s).
more infohttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792124/

Anti-Aquaporin 5 AntibodyAnti-Aquaporin 5 Antibody

Among the seven human aquaporins cloned to date(AQPs 0-6), genes encoding the four most closely related aquaporins(AQP0, AQP2, ... Aquaporin-5;AQP-5;AQP5;. Research Areas. ,signal transduction,metabolism,plasma membrane,channels, metabolism,types of disease, ... AQP5, and AQP6) have been mapped to chromosome band 12q13, suggesting an aquaporin family gene cluster at this locus. Aquaporin ... Background for Aquaporin-5(AQP-5). Aquaporin 5, also known as AQP5, is a water channel protein. The aquaporins(AQPs) are a ...
more infohttps://www.bosterbio.com/anti-aquaporin-5-antibody-pa1553.html

The effect of aquaporin-5 knockdown on HT29 colon cancer cell proliferation and migration |  OncologyPROThe effect of aquaporin-5 knockdown on HT29 colon cancer cell proliferation and migration | OncologyPRO

14P - The effect of aquaporin-5 knockdown on HT29 colon cancer cell proliferation and migration. ... Aquaporins (AQPs) are a family of integral membrane proteins, known for their role in fluid homeostasis. Overexpression of AQP5 ... The effect of aquaporin-5 knockdown on HT29 colon cancer cell proliferation and migration ... Simultaneous knockdown of both AQP1 and 5 may in fact be needed for improved inhibition of colon cancer cellular proliferation ...
more infohttps://oncologypro.esmo.org/Meeting-Resources/ESMO-Asia-2015-Congress/The-effect-of-aquaporin-5-knockdown-on-HT29-colon-cancer-cell-proliferation-and-migration

ELISA Kit for Aquaporin 5 (AQP5) - Human - BioTecNika PrimeELISA Kit for Aquaporin 5 (AQP5) - Human - BioTecNika Prime

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aquaporin 5 (AQP5) were tested 20 ... Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aquaporin 5 (AQP5) were tested on 3 ... This assay has high sensitivity and excellent specificity for detection of Aquaporin 5 (AQP5). ... No significant cross-reactivity or interference between Aquaporin 5 (AQP5) and analogues was observed. ...
more infohttps://labs.biotecnika.org/products/elisa-kit-for-aquaporin-5-aqp5-human

Plasma Membrane Abundance of Human Aquaporin 5 Is Dynamically Regulated by Multiple Pathways - pdf descargarPlasma Membrane Abundance of Human Aquaporin 5 Is Dynamically Regulated by Multiple Pathways - pdf descargar

Plasma Membrane Abundance of Human Aquaporin 5 Is Dynamically Regulated by Multiple Pathways. . Biblioteca virtual para leer y ... Aquaporin membrane protein channels mediate cellular water flow. Human aquaporin 5 AQP5 is highly expressed in the respiratory ... Plasma Membrane Abundance of Human Aquaporin 5 Is Dynamically Regulated by Multiple Pathways - Descarga este documento en PDF. ... Plasma Membrane Abundance of Human Aquaporin 5 Is Dynamically Regulated by Multiple Pathways. ...
more infohttp://libros.duhnnae.com/2017/jun9/149849579438-Plasma-Membrane-Abundance-of-Human-Aquaporin-5-Is-Dynamically-Regulated-by-Multiple-Pathways.php

Interactions with the aquaporin 5 gene increase the susceptibility to molar-incisor hypomineralization.  - PubMed - NCBIInteractions with the aquaporin 5 gene increase the susceptibility to molar-incisor hypomineralization. - PubMed - NCBI

Interactions with the aquaporin 5 gene increase the susceptibility to molar-incisor hypomineralization.. Pang L1, Li X2, Wang K ... In the Aquaporin 5(AQP5) gene, we individually analyzed two SNPs, rs1996315 and rs923911. We found no significant associations ...
more infohttps://phgkb.cdc.gov/PHGKB/phgHome.action?action=forward&dbsource=huge&id=184856

Evidence for the Expression of Aquaporin 5 in the Mammalian Conjunctival Epithelium  | IOVS | ARVO JournalsEvidence for the Expression of Aquaporin 5 in the Mammalian Conjunctival Epithelium | IOVS | ARVO Journals

Purpose: : To demonstrate in the conjunctiva the presence of aquaporin type 5 (AQP5), a water channel homologue found in the ... H. Oen, P. Cheng, H.C. Turner, O.A. Candia; Evidence for the Expression of Aquaporin 5 in the Mammalian Conjunctival Epithelium ... Evidence for the Expression of Aquaporin 5 in the Mammalian Conjunctival Epithelium ... Evidence for the Expression of Aquaporin 5 in the Mammalian Conjunctival Epithelium ...
more infohttp://iovs.arvojournals.org/article.aspx?articleid=2392750

Anti Aquaporin 5 antibody, anti Aquaporin5 antibody, Aquaporin-5 antibody, anti AQP5 antibody, AQP-5 antibodyAnti Aquaporin 5 antibody, anti Aquaporin5 antibody, Aquaporin-5 antibody, anti AQP5 antibody, AQP-5 antibody

... antibody for the specific detection of Aquaporin 5 (AQP5) by immunohistochemistry / western blot using Rabbit antibody to AQP5 ... A synthetic peptide from the c-terminal region of human Aquaporin 5 conjugated to blue carrier protein was used as the antigen. ...
more infohttps://www.osenses.com/-p-1836.html

The Aquaporin 5 -1364A/C Promoter Polymorphism Is Associated With Cytomegalovirus Infection Risk in Kidney Transplant...The Aquaporin 5 -1364A/C Promoter Polymorphism Is Associated With Cytomegalovirus Infection Risk in Kidney Transplant...

Background: The aquaporin 5 (AQP5) -1364A/C promoter single nucleotide polymorphism affects key mechanisms of inflammation and ... The Aquaporin 5 -1364A/C Promoter Polymorphism Is Associated With Cytomegalovirus Infection Risk in Kidney Transplant ... The Aquaporin 5 −1364A/C Promoter Polymorphism Is Associated With Cytomegalovirus Infection Risk in Kidney Transplant ... The Aquaporin 5 −1364A/C Promoter Polymorphism Is Associated With Cytomegalovirus Infection Risk in Kidney Transplant ...
more infohttps://phgkb.cdc.gov/PHGKB/phgHome.action?action=forward&dbsource=huge&id=184742

Frontiers | Overexpression of a Barley Aquaporin Gene, HvPIP2;5 Confers Salt and Osmotic Stress Tolerance in Yeast and Plants |...Frontiers | Overexpression of a Barley Aquaporin Gene, HvPIP2;5 Confers Salt and Osmotic Stress Tolerance in Yeast and Plants |...

We characterized an aquaporin gene HvPIP2;5 from Hordeum vulgare and investigated its physiological roles in heterologous ... We characterized an aquaporin gene HvPIP2;5 from Hordeum vulgare and investigated its physiological roles in heterologous ... Indeed, overexpression of HvPIP2;5 caused higher retention of chlorophylls and water under salt and osmotic stresses than did ... These results suggest that HvPIP2;5 overexpression brought about stress tolerance, at least in part, by reducing the secondary ...
more infohttps://www.frontiersin.org/articles/10.3389/fpls.2016.01566/full

Recent Articles | Aquaporins And Culture | The Scientist Magazine®| Page 5Recent Articles | Aquaporins And Culture | The Scientist Magazine®| Page 5

In Chapter 6, "The Hormonal Essence of the T-Rex?" author Cordelia Fine considers the biological dogma that testes, and the powerful hormones they exude, are the root of all sexual inequality.. 0 Comments. ...
more infohttps://www.the-scientist.com/?articles.list/tagNo/3378,3/tags/aquaporins,culture/pageNo/5/

AQP5 Gene - GeneCards | AQP5 Protein | AQP5 AntibodyAQP5 Gene - GeneCards | AQP5 Protein | AQP5 Antibody

Aquaporin 5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 ... The human Aquaporin-5 gene. Molecular characterization and chromosomal localization. (PMID: 8621489) Lee MD … Agre P (The ... AQP5 (Aquaporin 5) is a Protein Coding gene. Diseases associated with AQP5 include Palmoplantar Keratoderma, Bothnian Type and ...
more infohttp://www.genecards.org/cgi-bin/carddisp.pl?gene=AQP5

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Aquaporins (6)Ion Channels/genetics (3)Ion Channels/biosynthesis (2)Adenoviridae/genetics (1)Aging/metabolism (1)Aquaporins/ ... Aquaporins and the respiratory system: advice for a lung investigator Agre, Peter; Nielsen, Søren; King, Landon S. (American ... here we define aquaporin protein ontogeny in rat. Expression of aquaporin-3 (AQP3), AQP4, and ... ... Aquaporins in complex tissues. I. Developmental patterns in respiratory and glandular tissues of rat Agre, Peter; Nielsen, ...
more infohttps://jscholarship.library.jhu.edu/handle/1774.2/33532/discover?filtertype_0=subject&filtertype_1=author&filter_relational_operator_1=equals&filter_relational_operator_0=equals&filter_1=Agre%2C+Peter&filter_0=Membrane+Proteins&filtertype=has_content_in_original_bundle&filter_relational_operator=equals&filter=true

AQP5 - WikipediaAQP5 - Wikipedia

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 ... Aquaporin-5 is a protein that in humans is encoded by the AQP5 gene. Aquaporin 5 (AQP5) is a water channel protein. ... "Entrez Gene: AQP5 aquaporin 5". Verkman AS (2003). "Role of aquaporin water channels in eye function". Exp. Eye Res. 76 (2): ... 2003). "Distribution of aquaporin water channels AQP1 and AQP5 in the ductal system of the human pancreas". Gut. 52 (7): 1008- ...
more infohttps://en.wikipedia.org/wiki/AQP5
  • We report a fatal case of anti-aquaporin-4 antibody positive NMO spectrum disorder in a patient who was receiving treatment for pulmonary tuberculosis. (biomedcentral.com)
  • It shows the usefulness of testing for anti-aquaporin-4 antibodies while evaluating neurological deterioration in patients with tuberculosis. (biomedcentral.com)
  • Polymorphisms of aquaporin-5, a key mediator of inflammation, may impact outcome.What This Article Tells Us That Is NewIn acute respiratory distress syndrome attributable to bacterial pneumonia, the C-allele of the aquaporin-5 -1364A/C promoter polymorphism is associated with less pulmonary inflammation and greater survival. (medworm.com)
  • The Aquaporin 5 -1364A/C Promoter Polymorphism Is Associated With Cytomegalovirus Infection Risk in Kidney Transplant Recipients. (cdc.gov)
  • The primer used in the hybridization contains a T7 promoter sequence at its 5'-end. (google.ca)
  • Arabidopsis plants overexpressing HvPIP2;5 also showed better stress tolerance in germination and root growth under high salt and high osmotic stresses than the wild type (WT). (frontiersin.org)
  • Indeed, overexpression of HvPIP2;5 caused higher retention of chlorophylls and water under salt and osmotic stresses than did control. (frontiersin.org)
  • These results suggest that HvPIP2;5 overexpression brought about stress tolerance, at least in part, by reducing the secondary oxidative stress caused by salt and osmotic stresses. (frontiersin.org)
  • In addition, the proline biosynthesis genes, Δ 1 -Pyrroline-5-Carboxylate Synthase 1 and 2 ( P5CS1 and P5CS2 ) were up-regulated in HvPIP2;5 overexpressing plants under salt and osmotic stresses, which coincided with increased levels of the osmoprotectant proline. (frontiersin.org)
  • We also observed lower accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), an end-product of lipid peroxidation in HvPIP2;5 overexpressing plants than in WT. (frontiersin.org)
  • Stimulation of isolated acinar cells with carbachol (CCh), histamine or ATP was associated with marked decreases in cell volume (20 ± 2% within 62 ± 5 s) that were tightly correlated with increases in cytoplasmic Ca 2+ concentration ([Ca 2+ ] i ) as revealed by simultaneous DIC and fluorescent indicator dye microscopy. (wiley.com)
  • Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. (genecards.org)
  • 5 matching records from 5 references. (jax.org)