Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.
Peptide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Peptides whose amino and carboxy ends are linked together with a peptide bond forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS. Some of them are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL).
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Peptides composed of between two and twelve amino acids.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.
A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
The techniques used to produce molecules exhibiting properties that conform to the demands of the experimenter. These techniques combine methods of generating structural changes with methods of selection. They are also used to examine proposed mechanisms of evolution under in vitro selection conditions.
A PEPTIDE that is secreted by the BRAIN and the HEART ATRIA, stored mainly in cardiac ventricular MYOCARDIUM. It can cause NATRIURESIS; DIURESIS; VASODILATION; and inhibits secretion of RENIN and ALDOSTERONE. It improves heart function. It contains 32 AMINO ACIDS.
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
A highly basic, 28 amino acid neuropeptide released from intestinal mucosa. It has a wide range of biological actions affecting the cardiovascular, gastrointestinal, and respiratory systems and is neuroprotective. It binds special receptors (RECEPTORS, VASOACTIVE INTESTINAL PEPTIDE).
A technology, in which sets of reactions for solution or solid-phase synthesis, is used to create molecular libraries for analysis of compounds on a large scale.
Molecules of DNA that possess enzymatic activity.
Calcitonin gene-related peptide. A 37-amino acid peptide derived from the calcitonin gene. It occurs as a result of alternative processing of mRNA from the calcitonin gene. The neuropeptide is widely distributed in neural tissue of the brain, gut, perivascular nerves, and other tissue. The peptide produces multiple biological effects and has both circulatory and neurotransmitter modes of action. In particular, it is a potent endogenous vasodilator.
A 60-kDa extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.
Peptides that have the ability to enter cells by crossing the plasma membrane directly, or through uptake by the endocytotic pathway.
The rate dynamics in chemical or physical systems.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.
A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. Protein footprinting utilizes a protein cutting reagent or protease. Protein cleavage is inhibited where the proteins, or nucleic acids and protein, contact each other. After completion of the cutting reaction, the remaining peptide fragments are analyzed by electrophoresis.
A 36-amino acid peptide produced by the L cells of the distal small intestine and colon. Peptide YY inhibits gastric and pancreatic secretion.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
A PEPTIDE of 22 amino acids, derived mainly from cells of VASCULAR ENDOTHELIUM. It is also found in the BRAIN, major endocrine glands, and other tissues. It shares structural homology with ATRIAL NATRIURETIC FACTOR. It has vasorelaxant activity thus is important in the regulation of vascular tone and blood flow. Several high molecular weight forms containing the 22 amino acids have been identified.
Sites on an antigen that interact with specific antibodies.
Peptides that regulate the WATER-ELECTROLYTE BALANCE in the body, also known as natriuretic peptide hormones. Several have been sequenced (ATRIAL NATRIURETIC FACTOR; BRAIN NATRIURETIC PEPTIDE; C-TYPE NATRIURETIC PEPTIDE).
Established cell cultures that have the potential to propagate indefinitely.
Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15)
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Neuropeptide and gut hormone that helps regulate GASTRIC ACID secretion and motor function. Once released from nerves in the antrum of the STOMACH, the neuropeptide stimulates release of GASTRIN from the GASTRIN-SECRETING CELLS.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
A 27-amino acid peptide with histidine at the N-terminal and isoleucine amide at the C-terminal. The exact amino acid composition of the peptide is species dependent. The peptide is secreted in the intestine, but is found in the nervous system, many organs, and in the majority of peripheral tissues. It has a wide range of biological actions, affecting the cardiovascular, gastrointestinal, respiratory, and central nervous systems.
Ligases that catalyze the joining of adjacent AMINO ACIDS by the formation of carbon-nitrogen bonds between their carboxylic acid groups and amine groups.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
Cell surface receptors that bind peptide messengers with high affinity and regulate intracellular signals which influence the behavior of cells.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A potent natriuretic and vasodilatory peptide or mixture of different-sized low molecular weight PEPTIDES derived from a common precursor and secreted mainly by the HEART ATRIUM. All these peptides share a sequence of about 20 AMINO ACIDS.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Inorganic compounds that contain fluorine as an integral part of the molecule.
A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.
High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.
Proteins prepared by recombinant DNA technology.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.
Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Part of a MESSENGER RNA molecule that undergoes a conformation change upon binding a specific metabolite or other small molecule thereby regulating the messenger RNA's transcription, post-transcriptional processing, transport, translation, or stability in response to varying levels of the metabolite or other small molecule.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The molecular designing of drugs for specific purposes (such as DNA-binding, enzyme inhibition, anti-cancer efficacy, etc.) based on knowledge of molecular properties such as activity of functional groups, molecular geometry, and electronic structure, and also on information cataloged on analogous molecules. Drug design is generally computer-assisted molecular modeling and does not include pharmacokinetics, dosage analysis, or drug administration analysis.
A group of atoms or molecules attached to other molecules or cellular structures and used in studying the properties of these molecules and structures. Radioactive DNA or RNA sequences are used in MOLECULAR GENETICS to detect the presence of a complementary sequence by NUCLEIC ACID HYBRIDIZATION.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The use of devices which use detector molecules to detect, investigate, or analyze other molecules, macromolecules, molecular aggregates, or organisms.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The endogenous peptides with opiate-like activity. The three major classes currently recognized are the ENKEPHALINS, the DYNORPHINS, and the ENDORPHINS. Each of these families derives from different precursors, proenkephalin, prodynorphin, and PRO-OPIOMELANOCORTIN, respectively. There are also at least three classes of OPIOID RECEPTORS, but the peptide families do not map to the receptors in a simple way.
A cell line derived from cultured tumor cells.
Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
Hormones synthesized from amino acids. They are distinguished from INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS in that their actions are systemic.
The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Proteins that are chemically bound to a substrate material which renders their location fixed. The immobilization of proteins allows their use in chemical reactions without being diluted by solvent.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
Incorporation of biotinyl groups into molecules.
Peptides generated from AMYLOID BETA-PEPTIDES PRECURSOR. An amyloid fibrillar form of these peptides is the major component of amyloid plaques found in individuals with Alzheimer's disease and in aged individuals with trisomy 21 (DOWN SYNDROME). The peptide is found predominantly in the nervous system, but there have been reports of its presence in non-neural tissue.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)
The structure of one molecule that imitates or simulates the structure of a different molecule.
The processes of RNA tertiary structure formation.
A family of RNA plant viruses that infect a wide range of herbaceous and woody plant species. There are at least eight genera including POTEXVIRUS and CARLAVIRUS, both of which are highly immunogenic.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
A peptide of 36 or 37 amino acids that is derived from PROGLUCAGON and mainly produced by the INTESTINAL L CELLS. GLP-1(1-37 or 1-36) is further N-terminally truncated resulting in GLP-1(7-37) or GLP-1-(7-36) which can be amidated. These GLP-1 peptides are known to enhance glucose-dependent INSULIN release, suppress GLUCAGON release and gastric emptying, lower BLOOD GLUCOSE, and reduce food intake.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
The sum of the weight of all the atoms in a molecule.
Proteins found in any species of bacterium.
Cell surface proteins that bind VASOACTIVE INTESTINAL PEPTIDE; (VIP); with high affinity and trigger intracellular changes which influence the behavior of cells.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
Cell surface proteins that bind ATRIAL NATRIURETIC FACTOR with high affinity and trigger intracellular changes influencing the behavior of cells. They contain intrinsic guanylyl cyclase activity.
Proteins obtained from species in the class of AMPHIBIANS.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
Proteins and peptides found in SALIVA and the SALIVARY GLANDS. Some salivary proteins such as ALPHA-AMYLASES are enzymes, but their composition varies in different individuals.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The relationship between the dose of an administered drug and the response of the organism to the drug.
Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.
A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the short version of the genome and have a separate gene for cell lysis.
A multistage process that includes cloning, physical mapping, subcloning, sequencing, and information analysis of an RNA SEQUENCE.
The study of MAGNETIC PHENOMENA.
Antibodies produced by a single clone of cells.
The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)

Peptide aptamers targeting the hepatitis B virus core protein: a new class of molecules with antiviral activity. (1/64)

A substantial proportion of the worldwide liver cancer incidence is associated with chronic hepatitis B virus (HBV) infection. The therapeutic management of HBV infections is still problematic and novel antiviral strategies are urgently required. Using the peptide aptamer screening system, we aimed to isolate new molecules, which can block viral replication by interfering with capsid formation. Eight peptide aptamers were isolated from a randomized expression library, which specifically bound to the HBV core protein under intracellular conditions. One of them, named C1-1, efficiently inhibited viral capsid formation and, consequently, HBV replication and virion production. Hence, C1-1 is a novel model compound for inhibiting HBV replication by blocking capsid formation and provides a new basis for the development of therapeutic molecules with specific antiviral potential against HBV infections.  (+info)

Identification of the first Rho-GEF inhibitor, TRIPalpha, which targets the RhoA-specific GEF domain of Trio. (2/64)

The Rho-guanine nucleotide exchange factors (Rho-GEFs) remodel the actin cytoskeleton via their Rho-GTPase targets and affect numerous physiological processes such as transformation and cell motility. They are therefore attractive targets to design specific inhibitors that may have therapeutic applications. Trio contains two Rho-GEF domains, GEFD1 and GEFD2, which activate the Rac and RhoA pathways, respectively. Here we have used a genetic screen in yeast to select in vivo peptides coupled to thioredoxin, called aptamers, that could inhibit GEFD2 activity. One aptamer, TRIAPalpha (TRio Inhibitory APtamer), specifically blocks GEFD2-exchange activity on RhoA in vitro. The corresponding peptide sequence, TRIPalpha, inhibits TrioGEFD2-mediated activation of RhoA in intact cells and specifically reverts the neurite retraction phenotype induced by TrioGEFD2 in PC12 cells. Thus TRIPalpha is the first Rho-GEF inhibitor isolated so far, and represents an important step in the design of inhibitors for the expanding family of Rho-GEFs.  (+info)

The guanine nucleotide exchange factor trio activates the phagocyte NADPH oxidase in the absence of GDP to GTP exchange on Rac. "The emperor's nw clothes". (3/64)

The superoxide-generating NADPH oxidase complex of phagocytes consists of a membrane-associated flavocytochrome b(559) and four cytosolic components as follows: p47(phox), p67(phox), p40(phox), and the small GTPase Rac (1 or 2). Activation of the oxidase is the result of assembly of the cytosolic components with cytochrome b(559) and can be mimicked in vitro by mixtures of membrane and cytosolic components exposed to an anionic amphiphile, serving as activator. We reported that prenylation of Rac1 endows it with the ability to support oxidase activation in conjunction with p67(phox) but in the absence of amphiphile and p47(phox). We now show the following 6 points. 1) The Rac guanine nucleotide exchange factor Trio markedly potentiates oxidase activation by prenylated Rac1-GDP. 2) This occurs in the absence of exogenous GTP or any other source of GTP generation, demonstrating that the effect of Trio does not involve GDP to GTP exchange on Rac1. 3) Trio does not potentiate oxidase activation by prenylated Rac1-GTP, by nonprenylated Rac1-GDP in the presence or absence of amphiphile, and by a prenylated [p67(phox)-Rac1] chimera in GDP-bound form. 4) Rac1 mutants defective in the ability to bind Trio or to respond to Trio by nucleotide exchange fail to respond to Trio by enhanced oxidase activation. 5) A Trio mutant with conserved Rac1-binding ability but lacking nucleotide exchange activity fails to enhance oxidase activation. 6) The effect of Trio is mimicked by displacement of Mg(2+) from Rac1-GDP. These results reveal the existence of a novel mechanism of Rac activation by a guanine nucleotide exchange factor and suggest that the induction by Trio of a conformational change in Rac1, in the absence of nucleotide exchange, is sufficient for enhancing its effector function.  (+info)

Ultra high-speed sorting. (4/64)

BACKGROUND: Cell sorting has a history dating back approximately 40 years. The main limitation has been that, although flow cytometry is a science, cell sorting has been an art during most of this time. Recent advances in assisting technologies have helped to decrease the amount of expertise necessary to perform sorting. METHODS: Droplet-based sorting is based on a controlled disturbance of a jet stream dependent on surface tension. Sorting yield and purity are highly dependent on stable jet break-off position. System pressures and orifice diameters dictate the number of droplets per second, which is the sort rate limiting step because modern electronics can more than handle the higher cell signal processing rates. RESULTS: Cell sorting still requires considerable expertise. Complex multicolor sorting also requires new and more sophisticated sort decisions, especially when cell subpopulations are rare and need to be extracted from background. High-speed sorting continues to pose major problems in terms of biosafety due to the aerosols generated. CONCLUSIONS: Cell sorting has become more stable and predictable and requires less expertise to operate. However, the problems of aerosol containment continue to make droplet-based cell sorting problematical. Fluid physics and cell viability restraints pose practical limits for high-speed sorting that have almost been reached. Over the next 5 years there may be advances in fluidic switching sorting in lab-on-a-chip microfluidic systems that could not only solve the aerosol and viability problems but also make ultra high-speed sorting possible and practical through massively parallel and exponential staging microfluidic architectures.  (+info)

T2-TrpRS inhibits preretinal neovascularization and enhances physiological vascular regrowth in OIR as assessed by a new method of quantification. (5/64)

PURPOSE: A carboxyl-terminal fragment of tryptophan tRNA synthetase (T2-TrpRS) has demonstrated potent angiostatic activity during retinal developmental neovascularization in vivo. The effects of T2-TrpRS on pathologic neovascularization were tested and compared with a potent VEGF antagonist using the mouse model of oxygen-induced retinopathy (OIR). METHODS: C57BL/6J mice were transiently exposed to hyperoxic conditions (75% O2) between postnatal day 7 (P7) and P12 and then returned to room air. Retinas were isolated, blood vessels stained with isolectin Griffonia simplicifolia, images of retinal whole-mounts acquired, and the area of vascular obliteration and extent of preretinal neovascularization quantified. This method was compared to the commonly used method of OIR quantification in which the number of pre-inner limiting membrane (ILM) nuclei is counted in serial sections of whole eyes. To assess the angiostatic activity of T2-TrpRS, mice were injected intravitreally at P12 with either T2-TrpRS, a VEGF aptamer, or vehicle (PBS) alone, and the effects on area of obliteration and on preretinal neovascular tuft formation were assessed. RESULTS: Using a modified method of quantification in the mouse OIR model based on images of isolectin-stained retinal wholemounts, we were able to assess reliably and consistently both vascular obliteration and preretinal neovascular tuft formation in the same specimen. T2-TrpRS demonstrated potent angiostatic activity, reducing the appearance of pathologic neovascular tufts by up to 90%. Surprisingly, T2-TrpRS also enhanced physiological revascularization of the obliterated retinal vasculature, reducing these areas by up to 60% compared with PBS-injected eyes. In contrast, the VEGF antagonist, while similarly reducing preretinal neovascular tuft formation, did not enhance revascularization of the obliterated areas. CONCLUSIONS: Use of a rapid, quantifiable method to assess the effect of T2-TrpRS on retinal angiogenesis in the OIR model demonstrates the importance of a quantification system that permits simultaneous analysis of a drug's effect on vascular obliteration as well as on preretinal neovascularization. The results obtained using this method suggest enhanced clinical value for compounds such as T2-TrpRS that not only inhibit pathologic neovascularization, but also facilitate physiological revascularization of ischemic tissue.  (+info)

Peptide aptamer-mediated inhibition of target proteins by sequestration into aggresomes. (6/64)

Peptide aptamers (PAs) can be employed to block the intracellular function of target proteins. Little is known about the mechanism of PA-mediated protein inhibition. Here, we generated PAs that specifically bound to the duck hepatitis B virus (HBV) core protein. Among them, PA34 strongly blocked duck HBV replication by inhibiting viral capsid formation. We found that PA34 led to a dramatic intracellular redistribution of its target protein into perinuclear inclusion bodies, which exhibit the typical characteristics of aggresomes. As a result, the core protein is efficiently removed from the viral life cycle. Corresponding findings were obtained for bioactive PAs that bind to the HBV core protein or to the human papillomavirus-16 (HPV16) E6 protein, respectively. The observation that PAs induce the specific sequestration of bound proteins into aggresomes defines a novel mechanism as to how this new class of intracellular inhibitors blocks the function of their target proteins.  (+info)

Peptide aptamers that bind to a geminivirus replication protein interfere with viral replication in plant cells. (7/64)

The AL1 protein of tomato golden mosaic virus (TGMV), a member of the geminivirus family, is essential for viral replication in plants. Its N terminus contains three conserved motifs that mediate origin recognition and DNA cleavage during the initiation of rolling-circle replication. We used the N-terminal domain of TGMV AL1 as bait in a yeast two-hybrid screen of a random peptide aptamer library constrained in the active site of the thioredoxin A (TrxA) gene. The screen selected 88 TrxA peptides that also bind to the full-length TGMV AL1 protein. Plant expression cassettes corresponding to the TrxA peptides and a TGMV A replicon encoding AL1 were cotransfected into tobacco protoplasts, and viral DNA replication was monitored by semiquantitative PCR. In these assays, 31 TrxA peptides negatively impacted TGMV DNA accumulation, reducing viral DNA levels to 13 to 64% of those of the wild type. All of the interfering aptamers also bound to the AL1 protein of cabbage leaf curl virus. A comparison of the 20-mer peptides revealed that their sequences are not random. The alignments detected seven potential binding motifs, five of which are more highly represented among the interfering peptides. One motif was present in 18 peptides, suggesting that these peptides interact with a hot spot in the AL1 N terminus. The peptide aptamers characterized in these studies represent new tools for studying AL1 function and can serve as the basis for the development of crops with broad-based resistance to single-stranded DNA viruses.  (+info)

Inhibition of transforming growth factor-beta1-induced signaling and epithelial-to-mesenchymal transition by the Smad-binding peptide aptamer Trx-SARA. (8/64)

Overexpression of the inhibitory Smad, Smad7, is used frequently to implicate the Smad pathway in cellular responses to transforming growth factor beta (TGF-beta) signaling; however, Smad7 regulates several other proteins, including Cdc42, p38MAPK, and beta-catenin. We report an alternative approach for more specifically disrupting Smad-dependent signaling using a peptide aptamer, Trx-SARA, which comprises a rigid scaffold, the Escherichia coli thioredoxin A protein (Trx), displaying a constrained 56-amino acid Smad-binding motif from the Smad anchor for receptor activation (SARA) protein. Trx-SARA bound specifically to Smad2 and Smad3 and inhibited both TGF-beta-induced reporter gene expression and epithelial-to-mesenchymal transition in NMuMG murine mammary epithelial cells. In contrast to Smad7, Trx-SARA had no effect on the Smad2 or 3 phosphorylation levels induced by TGF-beta1. Trx-SARA was primarily localized to the nucleus and perturbed the normal cytoplasmic localization of Smad2 and 3 to a nuclear localization in the absence of TGF-beta1, consistent with reduced Smad nuclear export. The key mode of action of Trx-SARA was to reduce the level of Smad2 and Smad3 in complex with Smad4 after TGF-beta1 stimulation, a mechanism of action consistent with the preferential binding of SARA to monomeric Smad protein and Trx-SARA-mediated disruption of active Smad complexes.  (+info)

Kit 30500-050 Kit 30500-096 DNA marker 81-0020 DNA marker 81-0100 DNA marker 82-0100 DNA marker 82-0200 DNA marker 82-0500 DNA marker 82-1000 DNA marker 83-2500 DNA marker 83-5000 DNA Aptamers AD-155-B DNA Aptamers AD-155-F DNA Aptamers AD-155-U Peptide Aptamers AP-302-B Peptide Aptamers AP-302-F Peptide Aptamers AP-302-U Peptide Aptamers AP-304-B Peptide Aptamers AP-304-F Peptide Aptamers AP-304-U Peptide Aptamers AP-306-B Peptide Aptamers AP-306-F Peptide Aptamers AP-306-U Peptide Aptamers AP-308-B Peptide Aptamers AP-308-F Peptide Aptamers AP-308-U Peptide Aptamers AP-309-B Peptide Aptamers AP-309-F Peptide Aptamers AP-309-U Peptide Aptamers AP-310-B Peptide Aptamers AP-310-F Peptide Aptamers AP-310-U Peptide Aptamers AP-312-B Peptide Aptamers AP-312-F Peptide Aptamers AP-312-U Peptide Aptamers AP-315-B Peptide Aptamers AP-315-F Peptide Aptamers AP-315-U Peptide Aptamers AP-318-B Peptide Aptamers AP-318-F Peptide Aptamers AP-318-U Peptide Aptamers AP-319-B Peptide Aptamers AP-319-F Peptide Aptamers
Endothelial Cells (P36), Peptide Aptamer, FITC labelled Peptide Aptamers AP-308-F Endothelial Cells (P36), Peptide Aptamer, FITC labelled Peptide Aptamers AP-308-F
LMO2 is a transcription regulator involved in human T-cell leukemia, including some occurring in X-SCID gene therapy trials, and in B-cell lymphomas and prostate cancer. LMO2 functions in transcription complexes via protein-protein interactions involving two LIM domains and causes a preleukemic T-cell development blockade followed by clonal tumors. Therefore, LMO2 is necessary but not sufficient for overt neoplasias, which must undergo additional mutations before frank malignancy. An open question is the importance of LMO2 in tumor development as opposed to sustaining cancer. We have addressed this using a peptide aptamer that binds to the second LIM domain of the LMO2 protein and disrupts its function. This specificity is mediated by a conserved Cys-Cys motif, which is similar to the zinc-binding LIM domains. The peptide inhibits Lmo2 function in a mouse T-cell tumor transplantation assay by preventing Lmo2-dependent T-cell neoplasia. Lmo2 is, therefore, required for sustained T-cell tumor growth, in
TY - JOUR. T1 - Ultra-high sensitivity of the non-immunological affinity of graphene oxide-peptide-based surface plasmon resonance biosensors to detect human chorionic gonadotropin. AU - Chiu, Nan Fu. AU - Kuo, Chia Tzu. AU - Lin, Ting Li. AU - Chang, Chia Chen. AU - Chen, Chen Yu. PY - 2017/8/15. Y1 - 2017/8/15. N2 - Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing importance as sensing probes due to their potential in the development of non-immunological assays with high sensitivity, affinity and specificity for human chorionic gonadotropin (hCG) protein. We combined graphene oxide (GO) sheets with a specific peptide aptamer to create a novel, simple and label-free tool to detect abnormalities at an early stage of pregnancy, a GO-peptide-based surface plasmon resonance (SPR) biosensor. This is the first binding interface experiment to successfully demonstrate binding specificity in kinetic analysis biomechanics in peptide aptamers ...
PRF readers can get free access to a selected Journal of Pain paper each month, thanks to the American Pain Society. Get the free full text of the selection from the February 2018 issue here.. ...
The smooth identification and low-cost production of highly specific agents that interfere with signaling cascades by targeting an active domain in surface receptors, cytoplasmic and nuclear effector proteins, remain important challenges in biomedical research. We propose that peptide aptamers can provide a very useful and new alternative for interfering with protein-protein interactions in intracellular signal transduction cascades, including those emanating from activated receptors for growth factors. By their targeting of short, linear motif type of interactions, peptide aptamers have joined nucleic acid aptamers for use in signaling studies because of their ease of production, their stability, their high specificity and affinity for individual target proteins, and their use in high-throughput screening protocols. Furthermore, they are entering clinical trials for treatment of several complex, pathological conditions. Here, we present a brief survey of the use of aptamers in signaling pathways, in
The smooth identification and low-cost production of highly specific agents that interfere with signaling cascades by targeting an active domain in surface receptors, cytoplasmic and nuclear effector proteins, remain important challenges in biomedical research. We propose that peptide aptamers can provide a very useful and new alternative for interfering with protein-protein interactions in intracellular signal transduction cascades, including those emanating from activated receptors for growth factors. By their targeting of short, linear motif type of interactions, peptide aptamers have joined nucleic acid aptamers for use in signaling studies because of their ease of production, their stability, their high specificity and affinity for individual target proteins, and their use in high-throughput screening protocols. Furthermore, they are entering clinical trials for treatment of several complex, pathological conditions. Here, we present a brief survey of the use of aptamers in signaling pathways, in
Titanium (Ti) and titanium alloys (Ti6Al4V) are both used in clinical areas for example in orthopaedics, craniofacial, dental and bone replacement due to their exceptional mechanical properties tensile strength, stiffness, fracture toughness and corrosion resistance. The titanium oxide layer plays an important role on the surface of Ti allowing deposition of biomaterials, cells, proteins and peptides. Some studies suggested that a second bioactive material should be deposited, sprayed or attracted to the surface of Ti in order to increase its bioactivity. A well-known material for its high bioactivity is hydroxyapatite (HA)4 and is widely used as coating on implant surfaces. The aim of this project is to increase the interaction of nano hydroxyapatite and titanium oxide using an organic interphase of peptides known as aptamers.. ...
51556PRTAgrobacterium tumefaciens 1Met Asp Pro Lys Ala Glu Gly Asn Gly Glu Asn Ile Thr Glu Thr Ala 1 5 10 15 Ala Gly Asn Val Glu Thr Ser Asp Phe Val Asn Leu Lys Arg Gln Lys 20 25 30 Arg Glu Gly Val Asn Ser Thr Gly Met Ser Glu Ile Asp Met Thr Gly 35 40 45 Ser Gln Glu Thr Pro Glu His Asn Met His Gly Ser Pro Thr His Thr 50 55 60 Asp Asp Leu Gly Pro Arg Leu Asp Ala Asp Met Leu Asp Ser Gln Ser 65 70 75 80 Ser His Val Ser Ser Ser Ala Gln Gly Asn Arg Ser Glu Val Glu Asn 85 90 95 Glu Leu Ser Asn Leu Phe Ala Lys Met Ala Leu Pro Gly His Asp Arg 100 105 110 Arg Thr Asp Glu Tyr Ile Leu Val Arg Gln Thr Gly Gln Asp Lys Phe 115 120 125 Ala Gly Thr Thr Lys Cys Asn Leu Asp His Leu Pro Thr Lys Ala Glu 130 135 140 Phe Asn Ala Ser Cys Arg Leu Tyr Arg Asp Gly Val Gly Asn Tyr Tyr 145 150 155 160 Pro Pro Pro Leu Ala Phe Glu Arg Ile Asp Ile Pro Glu Gln Leu Ala 165 170 175 Ala Gln Leu His Asn Leu Glu Pro Arg Glu Gln Ser Lys Gln Cys Phe 180 185 190 Gln Tyr Lys Leu Glu Val Trp Asn Arg Ala His Ala Glu Met Gly Ile 195 200 ...
Platelets are the cellular components of the blood coagulation system. Among the proteins found at the surface of platelet plasma membrane, GPIIb-IIIa integrin harbors the human platelet antigens HPA-1a/b, the most clinically important platelet antigens. These antigens result from a leukine-proline polymorphism at position 33 of the GPIIb-IIIa integrin. About 2% of Caucasian women are homozygous (HPA-1b/1b) and risk forming antibodies against the integrin of the fetus. Such antibodies may destroy fetal platelets and lead to neonatal/fetal alloimmune thrombocytopenia (NAIT).1 Anti-platelet alloimmunization has an estimated incidence of 1 in 1,000 pregnancies and may cause in utero cerebral bleeds or ventriculomegaly.2-4 Thus, screening and identification of maternal alloantibodies are critical in early detection of such alloimmunization.5. Up to now, all methods for detecting auto- or alloantibodies directed at platelets, such as monoclonal antibody immobilization of platelet antigen assay ...
https://mallikaratchylab.org/wp-content/uploads/2016/08/mallikaratchy-banner-wide.png 0 0 Prabodhika Mallikaratchy https://mallikaratchylab.org/wp-content/uploads/2016/08/mallikaratchy-banner-wide.png Prabodhika Mallikaratchy2009-07-30 07:30:062019-05-22 19:52:41Cell-specific aptamer probes for membrane protein elucidation in cancer cells ...
The detection of biomarkers is of great significance in the diagnosis of numerous diseases, especially cancer. Herein, we developed a sensitive and universal fluorescent aptasensor strategy based on magnetic beads, DNA G-quadruplex, and exonuclease Ⅲ (Exo Ⅲ). In the presence of a target protein, a label-free single strand DNA (ssDNA) hybridized with the aptamer was released as a trigger DNA due to specific recognition between the aptamer and target. Subsequently, ssDNA initiates the Exo Ⅲ-aided recycling to amplify the fluorescence signal, which was caused by N-methylmesoporphyrin Ⅸ (NMM) insertion into the G-quadruplex structure. This proposed strategy combines the excellent specificity between the aptamer and target, high sensitivity of the fluorescence signal by G-quadruplex and Exo Ⅲaided recycling amplification. We selected (50-1200 nmol/L) MUC1, a common tumor biomarker, as the proof-of-concept target to test the specificity of our aptasensor. Results reveal that the sensor ...
Aptamers are an interesting class of molecules that have potential in many facets of human health. They are characterized by high affinity and specificity to their targets, are small in size, have similar properties to antibodies, but are made synthetically. All of these properties, among others, give aptamers the potential to diagnose, image and treat like no other molecules. By combining the unique properties of aptamers with the ever expanding field of nanotechnology and all it has to offer, we are entering a very promising new area of targeted nanodelivery treatments. These treatments have found success in the complex disease processes of cancer, eye and inflammatory diseases ...
... DUBLIN November 8 2013 /- ...Research and Markets ( a href http://www.researchandmarkets.com... (Logo: http://photos.prnewswire.com/prnh/2013...Aptamers Market - Technology Trend Analysis By Applications - Therapeu...,Global,Aptamers,Market,Technology,Trend,Analysis,Market,Report,2013-2018:,Therapeutics,,Diagnostics,,Biosensors,,Biomarker/Drug,Discovery,&,Applications,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
Antibodies have now been widely used for clinical treatment of a number of tumors. However, there are serious problems associated with antibody therapy, such as potential interactions of antibodies with the immune system as well as long production cycles. Recently, aptamers have been found to function similar to an
Aptamers Market was valued at USD 2142.8 Million in 2018 and is projected to reach around USD 7456.8 Million by 2025, at a CAGR...
In order to early diagnose breast cancer with the rapid quantification of carcinoembryonic antigen (CEA) in human serum, highly sensitive DNA aptasensor with 1,1-oxalyldiimidazole chemiluminescence (ODI-CL) detection was developed using the combination of CEA aptamer, [...] ...
Maize streak virus (MSV) disease (MSD) decimates tropical maize yields in subSaharan Africa reaching 100% crop loss. The disease, characterized by yellow streaks along leaf vasculature, delimits host photosynthesis leading to reduced or no grain filling, stunting and death in old and young infected plants respectively. Control of MSD via development of resistant maize varieties through classical breeding compromises other agronomical traits in the maize. The resistance is also poorly defined thus difficult to sustain or up-grade, allowing brisk virus counter resistance. There is need to compliment classical breeding basing on MSV virology coupled with comprehensible genetic manipulations. MSV replication proteins Rep and RepA have motifs that serve different but specific functions. For viral replication, the motifs involved locate in the N-terminus. Such functional interactions are subject to interference using various means, including synthetic short peptides termed peptide aptamers. The main ...
Human Immunodeficiency Virus (HIV) reverse transcriptase (RT) is the most common molecular target of current HIV treatments. Oligonucleotide aptamers bind and inhibit the RNA- and DNA-dependent polymerization activities of HIV RT. Libraries consisting of aptamers including 32, 70 or 80 nucleotide variable regions were previously screened by Systematic Evolution of Ligands by Exponential Enrichment (SELEX) against RT. Roughly half of the resulting aptamers were represented by pseudoknots with well defined signature sequences (the Family I), but also additional pseudoknots with little sequence convergence (Family II), and non-pseudoknot aptamers (Family III). Nucleic acid aptamers bind RT in the primer/template binding site. Aptamers are generally non-toxic and non-immunogenic molecules making them enticing drug prospects. Many aptamers inhibit DNA dependent DNA polymerization by RT from several phenotypically different recombinant viruses, but inhibition depends on a single amino acid mutation at ...
Background: RNA aptamers are small RNA molecules that bind antigens like antibodies and are currently being explored as alternatives to antibodies for diagnosis and therapy. A potential merit of aptamers is that they can be generated against native cellular antigens, such as those with unique post-translational modifications or receptor-ligand complexes, for which antibody generation can be difficult. Here, we report the use of a cell-based systematic enrichment approach (SELEX) to develop a novel Treg-binding RNA aptamer specific to IL2Rα-IL2 receptor-ligand complex.. Methods and Results:. A. Generation of Treg-binding aptamers:. We designed a cell-based SELEX strategy to generate RNA aptamers specific to human T regulatory (Treg) cells. The starting library consisted of random RNA aptamers with a structural diversity of ~1012. Aptamers against common T cell antigens were pre-cleared using CD4+CD25- Teff cells. Treg-binding aptamers were then positively selected using CD4+CD25+ Tregs from the ...
endothelial progenitor cells in a porcine myocardial infarction model. Nucleic Acid Therapeutics, 25: 20-26.. Ireson, C. and Kelland, L. (2006): Discovery and development of anticancer aptamers. Molecular Cancer Therapeuics, 5: 2957-2962.. Kaittanis, C., Santra, S. and Perez, J. (2010): Emerging nanotechnologybased strategies for the identification of microbial pathogenesis. Advanced Drug Delivery Reviews, 62: 408-442.Kanamori, H., Yuhashi, K., Uchiyama, Y., Kodama, T., and Ohnishi, S. (2009). In vitro selection of RNA aptamers that bind the RNAdependent RNA polymerase of hepatitis C virus: a possible role of GC-rich RNA motifs in NS5B binding. Virology, 388: 91-102.. Keefe, A., Pai, S. and Ellington, A. (2010). Aptamers as therapeutics. Nature Reviews Drug Discovery, 9: 537-550.. Kikuchi, K., Umehara, T., Fukuda, K., Hwang, J., Kuno, A., Hasegawa, T. and Nishikawa, S. (2003): RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region. Journal of ...
Infectious diseases are a subject of public health safety. In case of events such as bioterrorism or food samples tainted with a disease causing bacteria or virus the standard traditional methods of detection of viral or bacterial detection are too slow. We have developed molecular probes known as ?aptamers? to detect infection with high specificity and sensitivity. Aptamer, a word derived from Latin ?aptus? meaning ?to fit?; are molecular probes which are generated using nucleic acids which recognize and bind their target with a very high affinity and specificity. Aptamers are evolved in vitro in a test tube for its target. Aptamers are generated using a screening process known an SELEX, which stands for Systematic Evolution of Ligands by Exponential Enrichment. A library of 10 14 to 10 16 unique sequences is synthesized. These sequences are fractionated based on interactions with the target for which the aptamer is generated. The weaker binding sequences are weeded out after each successive ...
We have recently described the isolation of 2-fluoropyrimidine-substituted RNA aptamers that bind selectively to disease-associated beta-sheet-rich forms of the prion protein, PrP, from a number of mammalian species. These aptamers inhibit the accumulation of protease-resistant forms of PrP in a prion-seeded, in vitro conversion assay. Here we identify the minimal portions of two of these aptamers that retain binding specificity. We determine their secondary structures by a combination of modeling and solution probing. Finally, we identify an internal site for biotinylation of a minimized, synthetic aptamer and use the resultant reagent in the detection of abnormal forms of PrP in vitro.
Aptamers are dedicated oligonucleotides that bind in the active center of the polymerase and prevent attachment to nucleic acid targets at temperatures below the optimal reaction temperature of the Tth enzyme. Therefore, no primer elongation occurs during setup of the reaction and the following heating phase prior to the RT step. At higher temperatures, the Aptamers are released from the enzyme, and RT or DNA polymerization can be initiated. In addition, the recommended incubation temperature for RT with Tth (+61°C) is helpful to overcome secondary structures of RNA. This results in highly specific and efficient cDNA synthesis, which leads to highly specific and sensitive PCR ...
and / or polyclonal antibodies specific to a particular target for capture and / or quantitative detection. Most ELISAs employ a 96-well plate-based format. ELISAs are currently used in a wide range of industries, with wide-spread application for the detection of protein biomarkers in research, diagnostics and therapeutics. While antibody-based immunoassays have proven to be very sensitive and specific, there are some limitations which can be overcome with the ELASA, or Enzyme-Linked Aptamer Sorbent Assay. Unlike antibodies, aptamers can be selected for specific binding to poorly immunogenic and toxic compounds. Aptamers can also distinguish between highly conserved molecules. Chemical aptamer synthesis enables rapid, low-cost production of new batches with low lot-to-lot variability. As with traditional ELISAs, ELASAs can be direct, indirect, and sandwich assays. Several sandwich ELASA assays have been developed at Base Pair Biotechnologies. Biotinylated capture aptamers are typically bound to ...
Aptamers are single-stranded RNA or DNA oligonucleotides, which could be screened and synthesized for specific target (including any cell type), using systematic evolution of ligands by exponential enrichment (SELEX) technology..... Read More ...
Shigdar, Sarah, Lv, Li, Wang, Lifen and Duan, Wei 2016, Application of aptamers in histopathology. In Mayer, Gunter (ed), Nucleic acid aptamers : selection, characterization and application, Humana Press, New York, N.Y., pp.191-196, doi: 10.1007/978-1-4939-3197-2_16. ...
(EMAILWIRE.COM, March 20, 2017 ) According to the report Middle-East and Africa Aptamers Market published by Market Data Forecast, the Middle-East and African market was worth $9.53 million in 2016 and is projected to reach $11.82 million with CAGR of 15.0% by 2021 For full report refer to http://www.marketdataforecast.com/market-reports/middle-east-and-africa-aptamers-market-1139/ Aptamers...
Read A simple non-enzymatic strategy for adenosine triphosphate electrochemical aptasensor using silver nanoparticle-decorated graphene oxide, Journal of the Iranian Chemical Society on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
SelectScience visited the EDM Millipore booth at Neuroscience 2012 to find out more about the SNAP i.d.® 2.0 Protein Detection System. The new and improved SNAP i.d.® 2.0 Protein Detection System is the second generation of the SNAP i.d.® method for detecting immunoreactive proteins on western blots. With this unique vacuum-driven system, the length of time required for immunodetection is greatly reduced.
Aptamers are oligonucleotides that bind ligands-sometimes strongly and sometimes very specifically. They are, or will be, the basis of therapeutics, assays, and sensors. We consider how these molecules are designed and selected, and to what extent computational methods have helped or might help. ...
Aptamers, synthetic oligonucleotide‐based molecular recognition probes, have found use in a wide array of biosensing technologies based on their tight and highly selective binding to a variety of molecular targets
Adenosine/ATP (DH25.42), DNA Aptamer, unlabeled DNA Aptamers AD-104-U Adenosine/ATP (DH25.42), DNA Aptamer, unlabeled DNA Aptamers AD-104-U
Anti-His Tag (6H7), DNA Aptamer, FITC labeled DNA Aptamers AD-105-F Anti-His Tag (6H7), DNA Aptamer, FITC labeled DNA Aptamers AD-105-F
Quantify cytokines, chemokines, hormones, and other common disease indicating proteins with multiplex protein detection technology, Q-plex Arrays!
Minisatellite isoallelism, i.e. the occurrence of minisatellite alleles with different internal sequence composition but indistinguishable length, is a common limitation of minisatellite allele length analysis. Internal sequence variation can be used to distinguish such isoalleles, provided that detailed sequence knowledge of its basis is available. We now show that minisatellite isoalleles can also be simply resolved by single-stranded conformational polymorphisms (SSCP) arising during agarose gel electrophoresis. SSCP on agarose gels can be used to distinguish minisatellite isoalleles either after PCR amplification, or by standard Southern blot analysis of genomic DNA ...
An L-ribonucleic acid aptamer (L-RNA aptamer, trade name Spiegelmer - from German Spiegel mirror - by Noxxon Pharma) is an RNA-like molecule built from L-ribose units. It is an artificial oligonucleotide named for being a mirror image of natural oligonucleotides. L-RNA aptamers are a form of aptamers. Due to their L-nucleotides, they are highly resistant to degradation by nucleases. Spiegelmers are considered potential drugs and are currently being tested in clinical trials. Spiegelmers, built using L-ribose, are the enantiomers of natural oligonucleotides, which are made with D-ribose. Nucleic acid aptamers, including L-RNA aptamers, contain adenosine monophosphate, guanosine monophosphate, cytidine monophosphate, uridine monophosphate, a phosphate group, a nucleobase and a ribose sugar. Like other aptamers, L-RNA aptamers are able to bind molecules such as peptides, proteins, and substances of low molecular weight. The affinity of L-RNA aptamers to their target molecules often lies in the ...
Nucleic acid aptamers are short single-stranded DNA or RNA oligonucleotides that can bind to their targets with very high affinity and specificity, and are generally selected by a process referred to as systematic evolution of ligands by exponential enrichment. Conventional antibody-based therapeutic and diagnostic approach currently employed against biotoxins pose major limitations such as the requirement of a live animal for the in vivo enrichment of the antibody species, decreased stability, high production cost, and side effects. Aptamer technology is a viable alternative that can be used to combat these problems. Fully sequestered in vitro, aptamers eliminate the need for a living host. Furthermore, one of the key advantages of using aptamers instead of antibodies is that they can be selected against very weakly immunogenic and cytotoxic substances. In this review, we focus on nucleic acid aptamers developed against various biotoxins of plant, microorganism, or animal origin and show how ...
TY - JOUR. T1 - A sensitive method to detect Escherichia coli based on immunomagnetic separation and real-time PCR amplification of aptamers. AU - Lee, Hye Jin. AU - Kim, Byoung Chan. AU - Kim, Kyung Woo. AU - Kim, Young Keun. AU - Kim, Jungbae. AU - Oh, Min Kyu. PY - 2009/8/15. Y1 - 2009/8/15. N2 - Aptamers, single-stranded nucleic acids, provide a unique opportunity as amplifiable molecules using polymerase chain reaction (PCR) as well as recognition molecules like antibodies. We report a highly sensitive detection of Escherichia coli by taking advantage of the aptamer amplification as well as the specific binding of aptamers onto E. coli. This unique approach consists of three steps. First, the target E. coli was captured by antibody-conjugated magnetic beads. Second, the RNA aptamers were bound onto the surface of captured E. coli in a sandwich way. Finally, the heat-released aptamers were amplified by using real-time reverse-transcriptase-PCR (RT-PCR). The aptamer amplification in this ...
The functionality of aptamers is similar to that of antibodies. Aptamer are selected for specific target molecules by an in vitro selection and amplification method called SELEX. They can recognise and bind their targets with high affinity and specificity. As single-stranded oligonucleotides, aptamers are able to fold into complex and stable three-dimensional structures, which allow them to specifically interact with their targets. Aptamers are receiving increasing attention as alternative affinity reagents and represent essential tools in both basic and applied research. Aptamers can be used to detect and characterise their targets but also to modify the activity of their targets. Therefore, they provide a broad range of applications, e.g., affinity enrichment, analytics, medical diagnostics, or therapy.. Research focuses ...
70846 Oilseed contains sterols and related compounds with economic potential. The extraction and analysis of these compounds would be aided by the availability of highly selective aptamers with high affinities for their particular sterol ligands. This project will develop aptamers for use in microarrays to analyze and extract sterol contents of oil and other biological extracts. Bacterial expression vectors will be prepared from which the aptamers can be expressed in large quantities. In Phase I, one or more DNA aptamers for sitosterol will be selected. The aptamers will be evaluated for their specificity and affinity for sitosterol and related compounds. A bacterial expression vector will be developed from which aptamers can be prepared. Commercial Applications and Other Benefits as described by the awardee: Commercial applications include (1) the use of aptamers in the analysis and extraction of sitosterol and related compounds, and (2) a general method for economically mass-producing DNA ...
Aptamer Solutions Ltd is a York based Biotechnology Company specialising in the custom selection of high-affinity and highly specific nucleic acid aptamers for use in the life sciences sector. Our proprietary automated high-throughput aptamer selection processes allow us to offer a flexible and competitive pricing structure for the development of RNA and DNA aptamers. In addition, we are about to launch a new complementary technology in the area of biomarker discovery.. Our proprietary aptamer based biomarker discovery platform and proprietary combinatorial libraries contain up to and over 1018 different molecules, this diversity and bespoke library design is fundamental to the success of the screening process. This technology enables us to greatly speed up the identification of novel biomarkers as well as diagnostics and/or therapeutic candidate molecules. This technology builds on one of the most powerful uses of aptamer technology, which is the ability for aptamers to be isolated against ...
Aptamers are typically selected from libraries of random DNA (or RNA) sequences through systematic evolution of ligands by exponential enrichment (SELEX), which involves several rounds of alternating steps of partitioning of candidate oligonucleotides and their PCR amplification. Here we describe a protocol for non-SELEX selection of aptamers - a process that involves repetitive steps of partitioning with no amplification between them. Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM), which is a highly efficient affinity method, is used for partitioning. NECEEM also facilitates monitoring of bulk affinity of enriched libraries at every step of partitioning and screening of individual clones for their affinity to the target. NECEEM allows all clones to be screened prior to sequencing, so that only clones with suitable binding parameters are sequenced. The entire protocol can be completed in 1 wk, whereas conventional SELEX protocols take several weeks even in a specialized
View PROTEIN DETECTION for MB.BS.pptx from AA 1PROTEIN DETECTION Dr.Sajida Parveen Shaikh OBJECTIVES Define proteins List major body proteins in various body fluids. Proteinuria State Principle of
The adenosine aptamer was split into two halves and linked to a fluid liposome surface; addition of adenosine resulted in aptamer assembly, which did not occur if the split aptamer was attached to silica nanoparticles, demonstrating the feasibility of using aptamer probes to study diffusion within lipid membranes ...
A new Transparency Market Research report states that the global aptamers market stood at US$0.93 bn in 2012 and is predicted to reach US$4.3 bn by 2019. It...
The integration of anatomic, molecular, and genomic pathology into surgical pathology practice is conspicuous in oncology, where definition of molecular pathways important for specific tumors has enabled development of new biomarkers and innovative approaches to the detection of cancer and metastases. The so-called liquid biopsy includes a wide array of new technologies, including tumor-derived tumor vesicles and aptamer probes. The surgical pathologist will need to understand these new technologies and be aware of their advantages and pitfalls as they are applied into practice. Upon completion of this educational activity, participants should be able to:. ...
Please join EMD Millipore for a highly technical seminar describing two novel approaches for protein detection, the Direct Detect FTIR system and Multiplexing assays on the Luminex platform. The presentation is suitable for every level of scientist involved in protein research. Both of these technologies enable researchers to more fully understand the complexities of their cell/tissue samples by more accurately quantifying proteins and lipids, saving precious sample volume, and generating dozens of data points from a single biological sample.. Lunch will be provided. Registration is not required for this event.. ...
It was critically necessary to develop some delicate, handy and on-site strategies for simultaneous assay of various pathogenic micro organism in meals. On this work, a dual-mode aptasensor was established for fulfilling above goals combing colorimetry with microfluidic chip. This as-prepared dual-mode aptasensor not solely realized speedy screening by bare eye on-site, but additionally the … ...
Aptamer selection protocols, named cell-SELEX, have been developed to target trans-membrane proteins using whole living cells as target. This technique presents several advantages. (1) It does not...
Recently, they have improved the sensors reliability and sought new applications for the technology. To sense biological agents, the device takes rapid, direct measurements of the binding of an antigen to a chemical receptor on the waveguide surface. Researchers previously used antibodies as receptors. But they are more expensive and less reliable than aptamers, the synthetic, nucleic-acid-based receptors used in the sensor now, Campbell says. GTRI research scientist Jie Xu has been assisting Campbell with the aptamer work. ...
2.これまでの病型分類が当てはまらない胆道閉鎖症の症例 : 十二指腸への交通を認めた肝門部嚢胞型胆道閉鎖症(セッション2.「診断」,第37回日本胆道閉鎖症研究会) ...
低強度運動が冷水浸水時の体温調節反応に及ぼす影響―ふるえの深部体温閾値および感受性に着目して― ...
... s are a kind of peptide Aptamers. When the term mimotope was first coined by Mario Geysen in 1986, it was used to ... It had 10716 peptides grouped into 1229 sets. These peptides were extracted from biopanning results of phage-displayed random ... However, this concept has been extended to refer peptide mimic of all types of binding sites. As the mimic of binding site, ... A mimotope is a macromolecule, often a peptide, which mimics the structure of an epitope. Because of this property it causes an ...
Davis JJ, Tkac J, Humphreys R, Buxton AT, Lee TA, Ko Ferrigno P (May 2009). "Peptide aptamers in label-free protein detection: ... Davis JJ, Tkac J, Laurenson S, Ko Ferrigno P (February 2007). "Peptide aptamers in label-free protein detection: 1. ... Stabilisation of the two peptides by the protein scaffold constrains the possible conformations that the peptides can take, ... "Design and validation of a neutral protein scaffold for the presentation of peptide aptamers". Journal of Molecular Biology. ...
Known examples of smart ligands include DNA smart aptamers; however, RNA and peptide smart aptamers can also be developed. ... For example, a panel of DNA smart aptamers has been recently used to develop affinity analysis of proteins with ultra-wide ... Baaske P, Wienken CJ, Reineck P, Duhr S, Braun D (Feb 2010). "Optical Thermophoresis quantifies Buffer dependence of Aptamer ... Drabovich AP, Berezovski M, Okhonin V, Krylov SN (May 2006). "Selection of smart aptamers by methods of kinetic capillary ...
December 2007). "Microarray-formatted clinical biomarker assay development using peptide aptamers to anterior gradient-2". ...
Common address tags are monoclonal antibodies, aptamers, streptavidin or peptides. These targeting agents should ideally be ...
"Microarray-formatted clinical biomarker assay development using peptide aptamers to anterior gradient-2". Biochemistry. 46 (48 ...
... as in the case of aptamers, or from non-immunoglobulin protein scaffolds / peptide aptamers, into which hypervariable loops are ... 2005). "P. Design and validation of a neutral protein scaffold for the presentation of peptide aptamers". J Mol Biol. 352 (5): ... Within the protein scaffold there exist two variable peptide loops and a variable N-terminal sequence that provide a high ... PMID 24424458.CS1 maint: multiple names: authors list (link) Ladner, R.C. (1995). "Constrained peptides as binding entities". ...
"Selective inhibition of TGF-beta responsive genes by Smad-interacting peptide aptamers from FoxH1, Lef1 and CBP". Oncogene. 24 ...
SPR imaging platform would be a good choice to characterize aptamer -protein interactions. To study the aptamer-protein ... One can find protein-protein interaction and on-chip peptide distribution with high spatial resolution using subjected ... The interaction of thrombin and the aptamer can be monitored on microarray in real-time during injections of thrombin at ... Daniel, C; Roupioz, Y; Gasparutti, D; Livache, T; Buhot, A (2013). "Solution-Phase vs Surface-Phase Aptamer-Protein Affinity ...
... homogeneous pool of RNA aptamers can be produced. As such, RNA aptamers can be made to target small peptides and proteins, as ... RNA aptamers can be designed to act as antagonists, agonists, or so-called "RNA decoy aptamers." In the case of antagonists, ... For RNA decoy aptamers, the synthetic RNA aptamer resembles a native RNA molecule. As such, proteins(s) which bind to the ... Additionally, the term "aptamer" was coined by Andrew Ellington, who worked with Jack Szostak to select an RNA aptamer that was ...
"Prevention of Carbon Nanohorn Agglomeration Using a Conjugate Composed of Comb-Shaped Polyethylene Glycol and a Peptide Aptamer ... Another example is the specific peptide functionalized SWNHs nanocomposite, which was used to fabricate an immunosensor towards ...
Furthermore, the company offers Aptamers, small DNA, RNA (or peptide) molecules that show the ability to bind to specific ... "United States Patent - Streptavidin-binding peptide and uses thereof". "United States Patent - Peptides with sequentially ... Aptamers have been widely used in medical and industrial fields. 1996 - Institut für Bioanalytik GmbH was founded by Herbert ... Ho, D.; Falter, K.; Severin, P.; Gaub, H. E. (2009). "DNA as a force sensor in an aptamer-based biochip for adenosine". Anal ...
Antibodies, streptavidin, peptides, DNA, nucleic acid aptamers, or small-molecule ligands can be used to target quantum dots to ... Among these materials are InP/ZnS, CuInS/ZnS, Si, Ge and C. Peptides are being researched as potential quantum dot material. ... Whaley SR, English DS, Hu EL, Barbara PF, Belcher AM (2000). "Selection of peptides with semiconductor binding specificity for ... Hauser, Charlotte A. E.; Zhang, Shuguang (2010). "Peptides as biological semiconductors". Nature. 468 (7323): 516-517. Bibcode: ...
US granted 8685372, Roger Y. Tsien; Quyen T. Nguyen & Michael Whitney, "Peptides and aptamers for targeting of neuron or nerves ... PhD pertaining to their invention of peptides, imaging systems and methods to support fluorescence-guided cancer tumor ...
... transforming growth factor-beta1-induced signaling and epithelial-to-mesenchymal transition by the Smad-binding peptide aptamer ...
DNA aptamers, which are peptides that have with specific target molecules that they search for, fold in response to the ... Electrochemical Aptamer-Based Sensor". Journal of the American Chemical Society. 131 (12): 4262-4266. doi:10.1021/ja806531z. ... Aptamer-Based Tracking of Circulating Therapeutic Agents in Living Animals". Science Translational Medicine. 5 (213): 213ra165- ... this field was initiated with monitoring of small-molecule cocaine levels in undiluted blood serum with electrochemical aptamer ...
... es are often conceptually divided into two parts: an aptamer and an expression platform. The aptamer directly binds ... Two classes of glutamine riboswitches are known: the glnA RNA motif and Downstream-peptide motif. These classes are believed to ... as it contains contiguous dual aptamers. Though no longer shown to be cooperative, the cause of dual aptamers still remains ... of the relevant genes and the success of procedures to create artificial small molecule-binding RNAs called aptamers. In 2002, ...
... and the design of new sustainable pesticides in the form of peptide aptamers and small molecules that would interfere with cell ... 3) Research on computational agriculture including plant disease biocontrol studying anti-bacterial peptides expressed on virus ... Synthetic Cyclic KTS Peptides as Novel Dual Antagonists of α1β1/α2β1 Integrins with Antiangiogenic Activity". In Momic, Tatjana ...
... aptamers, and antimicrobial peptides) to enhance the limits of detection for certain analytes. Segal has helped engineer hybrid ... "Rapid and label-free detection of protein a by aptamer-tethered porous silicon nanostructures". Journal of Biotechnology. ... "Whole-cell detection of live lactobacillus acidophilus on aptamer-decorated porous silicon biosensors". Analyst. 141 (18): 5432 ...
Likewise, Xiao Ma and others, have discussed the electrical control on the binding/unbinding of thrombin from aptamers ... Biointerface science is a multidisciplinary field in which biochemists who synthesize novel classes of biomolecules (peptide ... "Resolving electrical stimulus triggered molecular binding and force modulation upon thrombin-aptamer biointerface". Journal of ... "Electrical Stimulus Controlled Binding/Unbinding of Human Thrombin-Aptamer Complex". Scientific Reports. 6: 37449. Bibcode: ...
... interaction mating and development of peptide aptamers) to detect and disrupt protein-protein interactions. In 1997, with ...
DNA aptamers, which are peptides that have with specific target molecules that they search for, fold in response to the ... "Real-Time, Aptamer-Based Tracking of Circulating Therapeutic Agents in Living Animals". Science Translational Medicine. 5 (213 ... this field was initiated with monitoring of small-molecule cocaine levels in undiluted blood serum with electrochemical aptamer ... "Continuous, Real-Time Monitoring of Cocaine in Undiluted Blood Serum via a Microfluidic, Electrochemical Aptamer-Based Sensor" ...
Antigenic peptide recognition on TAP was resolved by DNP-enhanced solid-state NMR spectroscopy. The conformational coupling and ... Furthermore, the guanine-sensing riboswitch-aptamer domain of the Bacillus subtilis xpt-pbuX operon, the Diels-Alderase ... Lehnert E, Mao J, Mehdipour AR, Hummer G, Abele R, Glaubitz C, Tampé R (2016). "Antigenic peptide recognition on the human ABC ... Thomas C, Tampé R (2017). "Structure of the TAPBPR-MHC I complex defines the mechanism of peptide loading and editing". Science ...
... in this method a peptide, antibody or aptamer are non-covalently bound to the enzyme at low temperature and inhibit its ...
... aptamers and ribozymes. In 2015, Jain et. al. described a trans-acting DNA-based amphiphatic delivery system for convenient ... Peptide nucleic acid (PNA) is an artificially synthesized polymer similar to DNA or RNA. Synthetic peptide nucleic acid ... Clicked peptide polymer Glycol nucleic acid Oligonucleotide synthesis Peptide synthesis Threose nucleic acid Nielsen PE, Egholm ... Zhao XL, Chen BC, Han JC, Wei L, Pan XB (November 2015). "Delivery of cell-penetrating peptide-peptide nucleic acid conjugates ...
Ames TD, Breaker RR (January 2011). "Bacterial aptamers that selectively bind glutamine". RNA Biol. 8 (1): 82-89. doi:10.4161/ ... Downstream-peptide RNAs are found upstream of short open reading frames (ORFs) that are predicted to encode short peptides ( ... Possible candidates to be regulated by the Downstream-peptide motif are genes that frequently carry the Downstream-peptide ... and their structural similarity to Downstream-peptide RNAs in turn suggests that Downstream-peptide RNAs are also riboswitches ...
Aptamer Deoxyribozyme Anti-thrombin aptamers Bacterial one-hybrid system Hak-Hagir A (September 1978). "[The Hak-Hagir skin ... "Short bioactive Spiegelmers to migraine-associated calcitonin gene-related peptide rapidly identified by a novel approach: ... Aptamers have emerged as a novel category in the field of bioreceptors due to their wide applications ranging from biosensing ... If a good binding affinity for the selected aptamer is not a concern, then an excess of target can be used to increase the ...
Among peptides obtained from biopannings, mimotopes can be considered as a kind of peptide aptamers. All the peptides panned ... Peptide aptamers. They consist of one (or more) short variable peptide domains, attached at both ends to a protein scaffold. ... Split aptamers are a potential template for biosensors in analogy to split protein systems. Peptide aptamers are artificial ... Peptide aptamers can also be selected from combinatorial peptide libraries constructed by phage display and other surface ...
Reference peptides are printed on the slides to allow for protein quantification of the sample lysates. RPAs allow for the ... In this technique, a library of antibodies, aptamers or affibodies is arrayed on the support surface. These are used as capture ... The capture molecules arrayed on the solid surface may be antibodies, antigens, aptamers (nucleic acid-based ligands), ... Shin, DS; Kim, DH; Chung, WJ; Lee, YS (September 30, 2005). "Combinatorial solid phase peptide synthesis and bioassays". ...
Like other aptamers, L-RNA aptamers are able to bind molecules such as peptides, proteins, and substances of low molecular ... L-RNA aptamers themselves have low antigenicity. In contrast to other aptamers, L-RNA aptamers have high stability in blood ... such as PEGylated L-RNA aptamers, show a prolonged plasma half-life. Unlike other aptamers, L-RNA aptamers are not directly ... L-RNA aptamers are a form of aptamers. Due to their L-nucleotides, they are highly resistant to degradation by nucleases. L-RNA ...
proapoptotic peptide against ANXA2 and prohibitin (Adipotide). *exotoxin against IL-2 (Denileukin diftitox) ... Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ...
Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ... Pituitary adenylate cyclase-activating peptide (PACAP). *Pleiotrophin. *Renalase. *Thrombopoietin (see here instead) ...
... is a pegylated anti-vascular endothelial growth factor (VEGF) aptamer, a single strand of nucleic acid that binds ... Pituitary adenylate cyclase-activating peptide (PACAP). *Pleiotrophin. *Renalase. *Thrombopoietin (see here instead) ... After the administration of the aptamer into rhesus monkeys, no toxic effects where exhibited. It was also noted that there was ...
The MeSH definition reads: "A well-characterized neutral peptide believed to be secreted by the liver and to circulate in the ... Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ...
Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ... Signaling peptide/protein receptor modulators. Cytokine receptor modulators. This article on a gene on human chromosome 1 is a ...
... s should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but ... "Controlling uncertainty in aptamer selection". Proceedings of the National Academy of Sciences of the United States of America ...
They are usually artificial peptides or proteins with a molar mass of about 3 to 20 kDa. Nucleic acids and small molecules are ... are connected to the variable domain of the heavy and light chain via a short linker peptide. The linker is rich in glycine, ...
EGF was originally described as a secreted peptide found in the submaxillary glands of mice and in human urine. EGF has since ... Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ...
... are neurotrophins which are released as biologically active uncleaved pro-peptides.[22] Unlike mature neurotrophins which bind ... Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ...
"Optical thermophoresis for quantifying the buffer dependence of aptamer binding". Angewandte Chemie. 49 (12): 2238-41. doi: ... Intercellular signaling peptides and proteins / ligands. Growth factors. *Epidermal growth factor. *Fibroblast growth factor ...
Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ... Intercellular signaling peptides and proteins / ligands. Growth factors. *Epidermal growth factor. *Fibroblast growth factor ...
Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ... Signaling peptide/protein receptor modulators. Cytokine receptor modulators. *. Molecular and Cellular Biology portal ...
Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ... Signaling peptide/protein receptor modulators. Cytokine receptor modulators. This article on a gene on human chromosome 19 is a ...
Allows construction of Cas9 complexes with protein binding cassettes, artificial aptamers, pools of random sequences as well as ... and inability to distinguish lncRNA function from other confounding factors like cryptically encoded peptides or functional DNA ... artificial aptamers and small molecules with varying size. While all the complexes were functional and viable, and successfully ...
Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ... Signaling peptide/protein receptor modulators. Cytokine receptor modulators. This biochemistry article is a stub. You can help ...
There is a debate about how long can HSP keep its peptide in extracellular space, at least for hsp70 the complex with peptide ... HSF1 inhibition by a potent RNA aptamer attenuates mitogenic (MAPK) signaling and induces cancer cell apoptosis.[34] ... This handing over with peptides is important, because HSPs can shield hydrophobic residues in peptides which would be otherwise ... and their peptide-binding sites were identified.[25] In the case of gp96 it is not clear whether it can bind peptides in vivo, ...
Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ... Signaling peptide/protein receptor modulators. Cytokine receptor modulators. This protein-related article is a stub. You can ...
... aptamer). Some transcripts act as ribozymes and self-regulate their expression. ...
between any kind of biomolecules[1] including proteins, DNA,[2] RNA,[3] peptides,[4] small molecules,[5] fragments[6] and ions ... "Optical thermophoresis for quantifying the buffer dependence of aptamer binding". Angew. Chem. Int. Ed. Engl. 49 (12): 1-5. doi ...
... (NTFs) are a family of biomolecules - nearly all of which are peptides or small proteins - that support ... Aptamers: Against NGF: RBM-004. *Decoy receptors: LEVI-04 (p75NTR-Fc) ... pituitary adenylate cyclase-activating peptide (PACAP), interleukin-1 (IL-1), interleukin-2 (IL-2), interleukin-3 (IL-3), ...
Heron SR, Wilson R, Shaffer SA, Goodlett DR, Cooper JM (May 2010). "Surface acoustic wave nebulization of peptides as a ... Superparamagnetic nanoparticles were immobilized with anti-IgE antibodies and fluorescently labeled aptamers to quantify IgE ... "High sensitive matrix-free mass spectrometry analysis of peptides using silicon nanowires-based digital microfluidic device". ...
While DNA is the dominant material used, structures incorporating other nucleic acids such as RNA and peptide nucleic acid (PNA ... and aptamers, which can bind to specific proteins or small molecules. Structural DNA nanotechnology, sometimes abbreviated as ... Emerging applications of DNAzymes and aptamers". Current Opinion in Biotechnology. 17 (6): 580-588. doi:10.1016/j.copbio. ...
One experiment conducted at the University of Florida led to the production of an XNA aptamer by the AEGIS-SELEX (artificially ... Peptide nucleic acid (PNA) FANA (Fluoro Arabino nucleic acid) HNA could be used to potentially act as a drug that can recognize ...
Bioinformatics screening and molecular docking analyses were utilized to select high affinity peptides targeting ... translationally controlled tumor protein (TCTP). Selected peptide aptamers were tested... ... Selected peptide aptamers were tested towards cancer cell lines with different levels of TCTP expression. One peptide (WGQWPYHC ... Cancer Fortilin Histamine releasing factor Leukemia Molecular docking Peptide aptamer TCTP This is a preview of subscription ...
Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume ... Isolation of Peptide Aptamers to Target Protein Function Luisa Lopez-Ochoa, Tara E. Nash, Jorge Ramirez-Prado, Linda Hanley- ... Peptide Aptamers for Small Molecule Drug Discovery Carine Bardou, Christophe Borie, Marc Bickle, Brian B. Rudkin, Pierre Colas ... In Nucleic Acid and Peptide Aptamers: Methods and Protocols, expert researchers contribute state-of-the-art methods focused on ...
Functionalization of biomedical surfaces by peptide aptamers.. In: 27th European Conference on Biomaterials, 30 Aug - 3 Sep ... to increase the interaction of nano hydroxyapatite and titanium oxide using an organic interphase of peptides known as aptamers ... proteins and peptides. Some studies suggested that a second bioactive material should be deposited, sprayed or attracted to the ...
... ... Here, we use a two hybrid approach to isolate from combinatorial libraries peptide aptamers that specifically interact with ... Our experiments argue that the variable region of this aptamer is structured, and that it functions by binding E2F with a motif ... These results show that cell proliferation can be inhibited using genetically-selected synthetic peptides that specifically ...
Nano-hydroxyapatite deposition on titanium using peptide aptamers.. In: 14th Annual Conference of the UK Society for ... to increase the interaction of nano hydroxyapatite and titanium oxide using an organic interphase of peptides known as aptamers ... peptide chemistry; surface science; hydroxyapatite; titanium; surface engineering; surface chemistry; orthopaedics; surface ... proteins and peptides. Some studies suggested that a second bioactive material should be deposited, sprayed or attracted to the ...
The peptide aptamer PA-2 was selected from pTRG-SN-peptides by bacterial two-hybrid system (B2H) employing pBT-SmpB or pBT-ArfA ... The peptide aptamer PA-2 was selected from pTRG-SN-peptides by bacterial two-hybrid system (B2H) employing pBT-SmpB or pBT-ArfA ... For identification of specific peptides that interact and inhibit the stalled-ribosome rescues, peptide aptamer library (pTRG- ... peptide aptamer library (pTRG-SN-peptides) was constructed using pTRG as vector and Staphylococcus aureus nuclease (SN) as ...
While previous research has shown aptamer-displaying PAMs enhance cell association, transportation to intracellular targets ... are a powerful platform technology for improving the delivery of therapeutic and prophylactic peptides. ... Therapeutic peptide delivery via aptamer-displaying, disulfide-linked peptide amphiphile micelles J. D. Smith, L. N. Cardwell, ... Peptide amphiphile micelles (PAMs) are a powerful platform technology for improving the delivery of therapeutic and ...
FITC labelled Peptide Aptamers AP-308-F Endothelial Cells (P36), Peptide Aptamer, FITC labelled Peptide Aptamers AP-308-F ... Endothelial Cells (P36), Peptide Aptamer, FITC labelled. Peptide Aptamers. 5 mg. Contact Sales ... Peptide Aptamer, FITC labelled (Peptide Aptamers) A-Z Product Search ... Endothelial Cells (P36), Peptide Aptamer, FITC labelled. Catalog#. Product Description. Product Type. Antibody Type. Size. ...
23 a peptide aptamer able to specifically bind this particular antibody should mimic the HPA-1a antigen. Such a peptide aptamer ... A peptide aptamer library was screened using an anti-HPA-1a human monoclonal antibody as a bait to isolate an aptamer that ... Screening of the peptide aptamer library. To set up a new detection assay for antiHPA-1a antibodies, we screened a peptide ... A peptide aptamer specific for human anti-HPA-1a antibodies. We describe the isolation and characterization of a peptide ...
Home , Papers , [EXPRESS] Primary sensory neuron-specific interference of TRPV1 signaling by AAV-encoded TRPV1 peptide aptamer ... EXPRESS] Primary sensory neuron-specific interference of TRPV1 signaling by AAV-encoded TRPV1 peptide aptamer attenuates ... EXPRESS] Primary sensory neuron-specific interference of TRPV1 signaling by AAV-encoded TRPV1 peptide aptamer attenuates ...
ATW0026 Aptamer to Human Latency Associated Peptide TGF-beta is secreted by many cell types including macrophages in a latent ... form in which it is complexed with two other polypeptides latent TGF-beta binding protein LTBP and latency associated peptide ... ATW0026 - Latency Associated Peptide (LAP) Aptamer. Amount* (µg):. Please, select one. 25 100 ... ATW0026 Aptamer to Human Latency Associated Peptide TGF-beta is secreted by many cell types, including macrophages, in a latent ...
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2. The peptide aptamer expression cassette of claim 1, further comprising a promoter. 3. The peptide aptamer expression ... 1. A peptide aptamer expression cassette comprising a DNA sequence encoding a polyprotein that constrains a peptide aptamer by ... 0005] Peptide aptamers are short peptides of random amino acid sequences. As commonly used, these peptides are generally 15-20 ... Peptide aptamers and the methods to produce cassettes including the aptamers and manipulating them, are described. The peptide ...
Treatment with the chemically-synthesized nucleolin aptamer-TAP siRNA conjugate represents a broadly-applicable approach to ... Administration of TAP siRNA conjugated to a broad-range tumor-targeting nucleolin aptamer inhibited tumor growth in multiple ... and induced the presentation of a TAP-independent peptide in human tumor cells. ... Aptamer-targeted siRNA downregulation of TAP in tumor cells. We first confirmed that the AS1411 nucleolin aptamer used in our ...
... ... social players for financial, additional, several and day-to-day view Nucleic Acid and Peptide Aptamers: Methods and Protocols ... learning the CAPTCHA s you are a simple and has you white view Nucleic Acid and Peptide Aptamers: to the community sample. ... This is Made view Nucleic Acid and Peptide Aptamers: Methods and Protocols and the co-operation holds enabled below chief. To ...
Aptamer Labeling. An aptamer comprising an aldehyde is conjugated to the Al-F-18 NOTA thiosemicarbazide of Example 21. The F-18 ... Al was added to the peptide and its HPLC profile compared to the empty NOTA peptide and the Al-F-18 peptide. The Al peptide and ... Peptide Synthesis. The peptides were synthesized by solid phase peptide synthesis using the Fmoc strategy. Groups were added to ... labeled peptide), two hours (77% labeled peptide) and four hours (76% labeled peptide) of incubation in human serum (not shown ...
2.How to design? Aptamers are oligonucleic acid or peptide molecules that bind to a specific target molecule. Aptamers are ... Aptamer Aptamer More details Details How to Select? Classification Features What is Aptamer? What is RNA Scaffold? Things left ... Researches on aptamers have achieved much progress in biosensor, drug development and nano technology. Normally 25-60 nt. ... High affinity and specificity Aptamers are selected by SELEX(systematic evolution of ligands by exponential enrichment) in ...
Peptide aptamers - artificial short peptides with specific binding affinity for target molecules - can be used to interfere ... Therefore, peptide aptamers have emerged as a new, powerful tool with high efficiency and specificity and wide applications in ... Peptide aptamers can also be applied to produce broad-spectrum viral resistance in plants. For example, based on the ... The authors further demonstrated that peptide aptamers can be extended to produce virus resistance in tomato (Solanum ...
Peptide aptamers - artificial short peptides with specific binding affinity for target molecules - can be used to interfere ... Therefore, peptide aptamers have emerged as a new, powerful tool with high efficiency and specificity and wide applications in ... Peptide aptamers can also be applied to produce broad-spectrum viral resistance in plants. For example, based on the ... The authors further demonstrated that peptide aptamers can be extended to produce virus resistance in tomato (Solanum ...
The selected aptamer was an 86-nucleotide DNA molecule that bound to an epitope peptide of HER2 with a Kd of 18.9 nM. The ... Figure 1. Evaluation of HER2 in breast cancer with the aptamer HB5. (a) Binding profiles of the aptamer HB5 to HER2-positive ( ... we propose a novel IHC method using a newly developed DNA aptamer (HB5). The aptamer HB5 was shown to be capable of ... Novel HER2 aptamer selectively delivers cytotoxic drug to HER2-positive breast cancer cells in vitro. J Transl Med 2012; 10: ...
The present invention provides an aptamer-based calorimetric sensor system for determining the presence and optionally the ... The aptamer 124 may be formed from deoxyribonucleotides, which may be natural, unnatural, or modified nucleic acids. Peptide ... After selecting an appropriate aptamer or aptamers in 120, a linker 128 is formed that includes the aptamer 124. In one aspect ... The aptamer selection 120 may provide one or more aptamers that demonstrate enhanced folding in the presence of the selected ...
sr.o. offers the synthesis of peptides with the chain length from 2 to 40 amino acid moieties in demanded purity. The ... For the peptide synthesis the recent technologies in the art are used, like automatic peptide synthesizer, microwave device and ... s r.o. offers the synthesis of peptides with the chain length from 2 to 40 amino acid moieties in demanded purity. The ... Usual amount of synthetised peptide is 5 to 100 mg, by mutual consent up to 1g. ...
A2-binding Peptide Epitopes 9.1.3 Monoclonal Antibodies in Cancer Therapy 9.1.4 Aptamers 9.1.5 Tumor Suppressors 9.1 Vaccines ... aptamers and Immunotherapy [9] Writer and Curator: Larry H. Bernstein, MD, FCAP This contribution has the following structure: ... including aptamer-drug conjugation, aptamer-nanoparticle conjugation, aptamer-mediated targeted gene therapy, aptamer-mediated ... Thus, this study utilized two aptamers, a 2′-fluoro modified RNA aptamer targeting PSMA-expressing cells and a DUP-1 peptide ...
Aptamers Market - Technology Trend Analysis By Applications - Therapeu...,Global,Aptamers,Market,Technology,Trend,Analysis, ... By Aptamer Type. - Nucleic acid aptamers. - Peptide aptamers. Key Topics Covered:. 1 Introduction. 2 Executive Summary. 3 ... the basics on nucleic acid aptamers, peptide aptamers and the SELEX method for their synthesis are also discussed. Research ... Aptamer Sciences Inc. - Aptamer Solutions Ltd. - Aptares AG. - Aptusbiotech. - Base Pair Biotechnologies. - Bioapter SL. - Cd ...
Therefore, aptamers that bind cell surface receptors have been exploited for the delivery of a variety of cargoes into cells. ... Therefore, relatively few aptamers have been generated that bind cell surface receptors. However, improvements in recombinant ... Several studies have demonstrated that aptamers that bind cell surface receptors may have functions other than just blocking ... This review focuses on recent progress and current challenges in the field of aptamer-mediated delivery. ...
Global aptamer market was valued at nearly US$1.0 bn in 2016 and is expected to expand with a CAGR of 20.0% over the forecast ... Aptamer Market Segmentation by Material Type - Nucleic acid Aptamer, Peptide Aptamer; Selection Technique Type - SELEX ... Based on material type the global aptamer market is segmented into nucleic acid aptamer and peptide aptamer. The material type ... Aptamer Market (Material - Nucleic acid Aptamer, Peptide Aptamer; Selection Technique - SELEX Technique and Others; Application ...
... J. Piccoli, R. Hein, A. H. El-Sagheer ... Herein we directly integrate receptive aptamers into a redox charging peptide support in enabling a label-free low picomolar ...
... peptides; aptamers; nucleic acid molecules (including, but not limited to, single stranded RNA or single-stranded DNA, or ... Aptamers can be made using methods described in U.S. Pat. No. 5,789,157 (herein incorporated by reference). Lectins, and ... aptamer, a nucleic acid molecule (e.g., RNA, DNA, hybrids, single stranded, double stranded, synthetic, oligonucleotides, etc ... protein or peptide or nucleic acid molecule) for a desired sequence ("combinatorial analysis"), and the like. ...
Nucleic Acid Aptamers: Selection, Characterization, and Application has 2 available editions to buy at Alibris ... Nucleic Acid Aptamers: Selection, Characterization, and Application by Gunter Mayer (Editor) starting at $109.96. ... Nucleic Acid and Peptide Aptamers: Methods and Protocols. by Gunter Mayer Starting at $72.57 ... This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers ...
9.3.2.2 Aptamers 219. 9.3.2.3 Proteins/Peptides 219. 9.3.2.4 Biopolymers 220 ... 10.3.1 Synthesis of Polyglycerol‐Functionalized Nanodiamond Conjugated with Platinum‐Based Drug and Targeting Peptide 274 ...
  • Design and Methods Peptide aptamer was used to detect and identify HPA-1a-specific antibodies in human serum that do not require human platelets. (haematologica.org)
  • Indeed, the peptide aptamer technology allows specific peptide ligands to be isolated for any given protein or domain, including antibodies. (haematologica.org)
  • Please note that aptamers have much lower molecular weights than antibodies. (basepairbio.com)
  • Aptamers existed alongside antibodies for two decades, but their market potential was not utilized to even a fraction of the same degree. (bio-medicine.org)
  • The list of promising aptamers in the clinical trials pipeline estimates that these synthetic chemical antibodies will soon surpass monoclonal antibodies in therapeutics, diagnosis, and imaging. (bio-medicine.org)
  • With technological merits over antibodies, the aptamers market is poised to grow at par with antibodies in the next 10-15 years. (bio-medicine.org)
  • Aptamers are developed from more reliable procedures than the manufacture of monoclonal antibodies. (transparencymarketresearch.com)
  • Aptamer can be used as alternative to antibodies for various application. (openpr.com)
  • Moreover, lack of universal rules for the application of aptamers in bioassays, which is well established when working with antibodies, restrains growth of global aptamer market. (openpr.com)
  • Aptamers are faster than antibodies in developing themselves and hence can help in curing the disease faster. (emailwire.com)
  • Our results show that peptide aptamers bear some analogies with monoclonal antibodies, with the advantages that they are isolated together with their coding genes, that their small size should allow their structures to be solved, and that they are designated to function inside cells. (wustl.edu)
  • Several reports have also described antinuclear antibodies cross-reacting with peptide self-antigens and depositing in the brain, kidneys, and skin [ 5 - 9 ]. (hindawi.com)
  • In mouse models of systemic lupus erythematosus, attempts were made to block the function of these cross-reacting antibodies using peptide aptamers, derived either from their cognate peptide self-antigens or from phage display libraries [ 10 , 11 ]. (hindawi.com)
  • Another viable approach to block antinuclear antibodies might be to use DNA aptamers, given the high-affinity of these antibodies for DNA and evidence of nucleotide base specificity. (hindawi.com)
  • But this approach has clearly been underexplored, perhaps due to the lack of reports on the feasibility of developing DNA aptamers to block the function of specific antibodies. (hindawi.com)
  • The other factors such as advancement in aptamer selection technologies coupled with various advantages of aptamers over monoclonal antibodies will further drive the market growth. (mynewsdesk.com)
  • Nucleic acid aptamers are nucleic acid species (next-gen antibody mimics) having selectivity at par of antibodies for a given target generated via in-vitro selection or equivalently, SELEX (systematic evolution of ligands by exponential enrichment) ranging from small entities such as heavy metal ions to large entities like cells. (wikipedia.org)
  • Aptamers are useful in biotechnological and therapeutic applications as they offer molecular recognition properties that rival that of the commonly used biomolecule, antibodies. (wikipedia.org)
  • In addition to their discriminate recognition, aptamers offer advantages over antibodies as they can be engineered completely in a test tube, are readily produced by chemical synthesis, possess desirable storage properties, and elicit little or no immunogenicity in therapeutic applications. (wikipedia.org)
  • In the past 30 to 40 years, synthesis technology has significantly improved to allow the development of a wide variety of polymers, and the application of functional molecules such as aptamers 5 and single-chain antibodies 6 has improved performance. (titech.ac.jp)
  • The protocols described range from well-established methods that have been used for a decade to generate high affinity antibodies, which are already in the clinic, to methods that are in their early stages of application such as display of peptides incorporating non-canonical amino acids. (springer.com)
  • Aptamer Group is a UK-based biotechnology company developing aptamers, nucleic acid molecules with affinity and selectivity for a specified target, as an alternative to antibodies. (onenucleus.com)
  • Aptamers can be used as pharmaceutical leads, detection reagents, functional proteomic levels with antibodies, and small molecules. (prsync.com)
  • 4. The system of claim 1 , wherein said plurality of receptor probes comprise biomimetics selected from the group consisting of molecular imprint antibodies, DNA-based aptamers, PNA, cyclodextrixis and dendrimers. (google.com)
  • Antibodies and aptamers are preselected and appropriately prepared for radiolabeling procedures. (nih.gov)
  • Validated and well characterized affinity capture reagents ( e.g. antibodies, aptamers, and affibodies) will play a key role in proteomics research platforms for the prevention, early detection, treatment, and monitoring of cancer. (mcponline.org)
  • The National Cancer Institute (NCI) 1 convened the "Proteomic Technologies Reagents Resource Workshop" in Chicago, IL on December 12-13, 2005 to identify the cancer research community's expressed needs for validated and well characterized affinity capture reagents ( e.g. antibodies, aptamers, and affibodies) to advance proteomics research platforms for the prevention, early detection, treatment, and monitoring of cancer. (mcponline.org)
  • You may already be familiar with peptides or proteins, such as antibodies, that bind to specific molecules. (mit.edu)
  • Like monoclonal antibodies, aptamers offer both selectivity and affinity for specific targets. (cambio.co.uk)
  • Unlike antibodies, aptamers can be selected for specific binding to poorly immunogenic and toxic compounds. (cambio.co.uk)
  • High affinity and specificity Aptamers are selected by SELEX(systematic evolution of ligands by exponential enrichment) in vitro. (prezi.com)
  • A novel HER2 aptamer (HB5) has recently been developed using systematic evolution of ligands by exponential enrichment technology (SELEX). (nature.com)
  • SELEX products were named 'aptamers,' which are single-stranded RNA or DNA oligonucleotides that bind with high affinity to specific targets such as proteins, small molecules, nucleic acids and cells. (nature.com)
  • As well as a discussion on the properties of aptamers in the technological analysis, the basics on nucleic acid aptamers, peptide aptamers and the SELEX method for their synthesis are also discussed. (bio-medicine.org)
  • On the basis of the selection technique, the market is segmented into the SELEX technique and other techniques (X-aptamer and MARAS technique). (transparencymarketresearch.com)
  • Among these, SELEX technique is expected to account for the leading share in the global market for aptamer over the forecast period from 2017 to 2025. (transparencymarketresearch.com)
  • This growth is attributable to the advancement of the techniques such as CE-SELEX and M-SELEX which helps to overcome some of the limitations in the other procedures for selection of aptamers. (transparencymarketresearch.com)
  • The procedure commonly used for the selection of aptamers for a specific target molecule is known as SELEX process. (transparencymarketresearch.com)
  • SELEX technique is likely to get boost for the modification of existing SELEX process, and available of the new updated SELEX technique like CE-SELEX, M- SELEX etc. which overcome the certain limitation for aptamer selection. (openpr.com)
  • Such aptamers can be isolated from combinatorial libraries by SELEX (systematic evolution of ligands by exponential enrichment) [ 3 ]. (mdpi.com)
  • Generated by Systematic Evolution of Ligands by Exponential Enrichment (SELEX), aptamers are short synthetic oligonucleotides, either DNA or RNA, that specifically bind target molecule(s) with high specificity due to their specific and complex three-dimensional shape comprised stems, loops, bulges, hairpins, pseudoknots, triplexes, and quadraplexes [ 12 - 14 ]. (hindawi.com)
  • Using SELEX (systematic evolution of ligands by exponential enrichment), we serendipitously discovered a ssDNA aptamer that binds selectively to the anti-FLAG M2 antibody. (hindawi.com)
  • These findings demonstrate the feasibility of using SELEX to develop ssDNA aptamers that block the function of a specific antibody, a capability that could lead to the development of novel therapeutic modalities for patients with systemic lupus erythematosus, rheumatoid arthritis, and other autoimmune diseases. (hindawi.com)
  • Normally aptamers can be generated by the aptamer selection process known as SELEX process. (mynewsdesk.com)
  • Several modifications of the SELEX procedure have been introduced to improve aptamer selectivity or to reduce selection time. (mynewsdesk.com)
  • Subtractive SELEX, automated SELEX and atomic force microscopy-SELEX are some of the modified SELEX processes available for aptamer selection with desired features. (mynewsdesk.com)
  • Generally, aptamers can be artificially generated in vitro by a process commonly referred to as SELEX (Systematic Evolution of Ligands by Exponential Enrichment) [ 18 ], which consists of several repeated rounds of binding, partition, and amplification [ 2 ]. (biomedcentral.com)
  • In SELEX experiments, aptamers are identified for their abilities to bind a protein of interest from libraries containing up to 10 16 different RNA or DNA sequences [ 19 ]. (biomedcentral.com)
  • Because SELEX typically takes several rounds to isolate target-binding aptamers, you will start with a known aptamer mixture rather than than a completely random library. (mit.edu)
  • The immense diversity in function and structure of nucleic acids enable numerous aptamers to be generated through an iterative in vitro selection technique known as Systematic Evolution of Ligands by EXponential enrichment (SELEX). (scirp.org)
  • More complex aptamer identification methods, using whole cancer cells (Cell-SELEX), may identify novel targets and aptamers to affect them. (scirp.org)
  • Nucleic acid aptamers are engineered through SELEX or in vitro selection. (antibodies-online.com)
  • These molecules were found to self-assemble in water into PAMs for which lipidated DNA oligomers ( i.e., antitail amphiphiles - AAs) could be entrapped and used to tether aptamers (Apt) to the nanoparticle surface. (rsc.org)
  • Thus, there are typically five to fifteen times as many moles or molecules of aptamers present in a solution containing an equal mass. (basepairbio.com)
  • In particular embodiments, the labeled molecules may be peptides or proteins, although other types of molecules including but not limited to aptamers, oligonucleotides and nucleic acids may be labeled and utilized for such imaging studies. (freepatentsonline.com)
  • Aptamers are oligonucleic acid or peptide molecules that bind to a specific target molecule. (prezi.com)
  • Though there are just approximately 60 companies (biotech and pharmaceutical) working on aptamers, there are quite a number of research groups focused on the diverse applications of aptamers, particularly in studying key target molecules involved in cardiovascular diseases, infectious diseases, and against viral antigens. (bio-medicine.org)
  • Binding sites for aptamers include clefts and grooves of target molecules (including enzymes) resulting in antagonistic activity very similar to many currently available pharmaceutical agents. (mdpi.com)
  • Aptamers are special class of molecules that combine the advantages of both low molecular weight and protein molecules. (transparencymarketresearch.com)
  • Aptamers illustrate lock-and-key relationship between aptamer molecules and binding partners i.e., target molecules. (transparencymarketresearch.com)
  • They called these RNA sequences-and later similarly behaving and selected single-stranded DNA molecules [ 2 ]-aptamers. (mdpi.com)
  • Aptamers are oligonucleotides that bind to target lipid, protein, or nucleic acid molecules with a high specificity. (emailwire.com)
  • Aptamers are able to bind a wide variety of targets from divalent metal ions, small organic molecules, proteins, and cells [ 15 , 16 ]. (hindawi.com)
  • Aptamers (from the Latin aptus - fit, and Greek meros - part) are oligonucleotide or peptide molecules that bind to a specific target molecule. (wikipedia.org)
  • Extension of aptamer selection from natural components to from unnatural components realizes the creation of new functional molecules. (rsc.org)
  • The immobilization of a vast number of substances and molecules, ranging from inorganic calcium phosphate phases up to peptides and proteins, has been investigated throughout recent decades. (royalsocietypublishing.org)
  • offering customers access to an accelerated aptamer selection platform that delivers tailor-made high-affinity, highly specific molecules for use in a variety of scenarios. (onenucleus.com)
  • Aptamers, first reported by Ellington and Gold in 1990 [ 1 , 2 ], are single stranded DNA/RNA molecules or peptide molecules [ 3 ]. (biomedcentral.com)
  • Aptamers are DNA, RNA or peptide molecules that bind to specific molecular targets. (thehindubusinessline.com)
  • 17. A method according to claim 1 wherein said immobilized probe molecules are peptides. (google.co.uk)
  • Aptamers are nucleic acid or peptide based binders that specifically recognized proteins, nucleic acids, and other molecules. (antibodies-online.com)
  • Aptamers are single-stranded DNA or RNA molecules that can bind to pre-selected targets including proteins and peptides with high affinity and specificity. (cambio.co.uk)
  • Aptamer affinity chromatography has been used to purify small molecules, proteins, and even for cell separation. (cambio.co.uk)
  • Aptamers can also distinguish between highly conserved molecules. (cambio.co.uk)
  • The antisense nucleotide method comprises targeting specific nucleotides rather than proteins.Besides, oligonucleic acid or peptide molecules that bind to a particular target molecule is known as Aptamer. (epicos.com)
  • Aptamers are a class of therapeutic oligonucleotides that form specific three-dimensional structures that are dictated by their sequences. (mdpi.com)
  • In contrast to antisense oligonucleotides and small interfering RNAs (siRNAs) that inhibit translation of proteins by Watson-Crick base-pairing to their respective messenger RNAs, aptamers bind to existing proteins (and, less commonly, non-protein targets) with high affinity and specificity, analogous to monoclonal antibodies. (mdpi.com)
  • The chemically synthesized single-standard oligonucleotides (a polynucleotide which contains a relatively small number of nucleotides) are aptamers. (transparencymarketresearch.com)
  • Aptamers are chemically synthesized single stranded oligonucleotides that are used to inhibit variety of target proteins and antigens. (mynewsdesk.com)
  • Nucleic acid aptamers have the strand of oligonucleotides while peptide aptamers contain short peptide domain in its structure. (mynewsdesk.com)
  • We isolated specifically interacting oligonucleotides, and biochemical strategies were used to identify the protein target for one of the aptamers. (pnas.org)
  • The most accessible techniques are based on the phage display of peptide or antibody libraries ( 4 - 8 ) and the use of libraries of oligonucleotides ( 9 , 10 ). (pnas.org)
  • Aptamers are single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) oligonucleotides, which are able to bind their target with high selectivity and affinity. (smw.ch)
  • Aptamers are single stranded DNA or RNA oligonucleotides whose three-dimensional structures are dictated by their sequences. (scirp.org)
  • d) Aptamers are short DNA or RNA oligonucleotides, or peptides that have a stable three‐dimensional structure in vivo . (els.net)
  • Apart from antiviral drugs, some alternative approaches are used for the treatment of viral diseases.These approaches include antisense oligonucleotides, aptamers, immunomodulators, vaccines, and ribozymes. (epicos.com)
  • A significant proportion of these oligonucleotides display G-quadruplex structures, particularly for DNA aptamers, that enable molecular recognition of their ligands. (eurekaselect.com)
  • Peptide aptamers are recombinant proteins which can interact with any given protein target with high specificity. (haematologica.org)
  • The nucleolin aptamer used in our study as prototype of tumor-targeting ligand, exhibits specificity to a broad range of tumor cells of both murine and human origin, and was used to target biological agents to tumor cells in mice 17 . (nature.com)
  • By their targeting of short, linear motif type of interactions, peptide aptamers have joined nucleic acid aptamers for use in signaling studies because of their ease of production, their stability, their high specificity and affinity for individual target proteins, and their use in high-throughput screening protocols. (mdpi.com)
  • Aptamers can be engineered to have higher specificity and affinity for number of targets. (emailwire.com)
  • The binding specificity and affinity of aptamers have long been harnessed as the key elements in the development of aptamer-based assays, particularly aptasensing application. (hindawi.com)
  • One promising avenue that is currently explored based on the specificity and affinity of aptamers is the application of aptamers in the decontamination assays. (hindawi.com)
  • Binding specificity and affinity of aptamers are harnessed in various applications, majorly in biosensing applications [ 10 , 11 , 17 - 26 ]. (hindawi.com)
  • Peptides are designed with consideration of selectivity of radiolabel incorporation and specificity for the intended target. (nih.gov)
  • Nanoparticles that are suitable for detection and loading with drugs can be directed to the site of their action by use of the affinity and specificity of aptamers, which are conjugated to the nanoparticles or fixed on their surface. (smw.ch)
  • Aptamers can facilitate cell-specific drug delivery in a selective manner to the sick cancer cells because of their binding specificity. (smw.ch)
  • Short fragments of RNA - called RNA aptamers - can have secondary structures that also allow them to bind a target molecule with good affinity and specificity. (mit.edu)
  • X-aptamers incorporate chemically modified nucleotides and DNA backbones to achieve a greater range of possible targets or increase affinity and specificity. (antibodies-online.com)
  • Aptamers with the desired specificity are selected using the yeast two hybrid system or established display techniques. (antibodies-online.com)
  • The titanium oxide layer plays an important role on the surface of Ti allowing deposition of biomaterials, cells, proteins and peptides. (open.ac.uk)
  • A major problem in drug delivery is the effective absorption of high molecular weight material such as proteins and peptides across biological membranes. (google.es)
  • Nucleic acid aptamers are short, single-stranded RNAs and DNAs that represent high-affinity and highly selective ligands for different targets, ranging from large proteins to peptides, nucleotides, drugs and small compounds, and even metal ions. (mdpi.com)
  • The Szostak lab also coined the term aptamer (from the Latin, apto, meaning 'to fit') for these nucleic acid-based ligands. (wikipedia.org)
  • Two years later, the Szostak lab and Gilead Sciences, independent of one another, used in vitro selection schemes to evolve single stranded DNA ligands for organic dyes and human coagulant, thrombin (see anti-thrombin aptamers), respectively. (wikipedia.org)
  • While the process of artificial engineering of nucleic acid ligands is highly interesting to biology and biotechnology, the notion of aptamers in the natural world had yet to be uncovered until 2002 when two groups led by Ronald Breaker and Evgeny Nudler discovered a nucleic acid-based genetic regulatory element (which was named riboswitch) that possesses similar molecular recognition properties to the artificially made aptamers. (wikipedia.org)
  • Lately, a concept of smart aptamers, and smart ligands in general, has been introduced. (wikipedia.org)
  • Our goal was to generate oligonucleotide ligands that recognize tumor-associated proteins on/within living cells, simultaneously identifying target proteins and DNA aptamers. (pnas.org)
  • One area of interest is high-diversity polymer libraries, including aptamers and peptides, which can be screened to identify high-affinity cancer ligands. (hopkinsmedicine.org)
  • Here, we review DNA G-quadruplex aptamers and their ligands from a structural and functional perspective. (eurekaselect.com)
  • [0004] Aptamers are nucleic or amino acid macromolecules that may he designed to bind tightly to specific targets. (patentsencyclopedia.com)
  • Therefore, relatively few aptamers have been generated that bind cell surface receptors. (mdpi.com)
  • Several studies have demonstrated that aptamers that bind cell surface receptors may have functions other than just blocking receptor-ligand interactions. (mdpi.com)
  • Therefore, aptamers that bind cell surface receptors have been exploited for the delivery of a variety of cargoes into cells. (mdpi.com)
  • First, most aptamers bind to targets with high affinity, demonstrating typical dissociation constants in the pico- to nanomolar range. (mdpi.com)
  • We report here the expression of a combinatorial library of constrained 20-residue peptides displayed by the active-site loop of Escherichia coli thioredoxin, and the use of a two-hybrid system to select those that bind human Cdk2. (wustl.edu)
  • Aptamers specifically bind to a range of micromolar to macromolar molecular targets. (mynewsdesk.com)
  • On the molecular level, aptamers bind to its cognate target through various non-covalent interactions viz. (wikipedia.org)
  • First, both Downstream-peptide RNAs and glnA RNAs selectively bind glutamine. (wikipedia.org)
  • Here we present a platform where short unstructured peptides that bind to a desired target are selected using M13 phage display. (ebscohost.com)
  • Their aptamer, artificially evolved to bind tightly to the gp120 molecule on the surface of HIV, interrupts cell infection by the virus by itself. (sciencemag.org)
  • Bioinformatics screening and molecular docking analyses were utilized to select high affinity peptides targeting translationally controlled tumor protein (TCTP). (springer.com)
  • These results show that cell proliferation can be inhibited using genetically-selected synthetic peptides that specifically target protein-protein interaction motifs within cell cycle regulators. (nih.gov)
  • For identification of specific peptides that interact and inhibit the stalled-ribosome rescues, peptide aptamer library (pTRG-SN-peptides) was constructed using pTRG as vector and Staphylococcus aureus nuclease (SN) as scaffold protein, in which 16 random amino acids were introduced to form an exposed surface loop. (frontiersin.org)
  • To assess their enhanced intracellular delivery capacity and therapeutic functionality, PAMs bearing cell-penetrating peptide modified "Plenty of SH3 domains" scaffold protein competitive inhibitor (Tat-POSH) and B cell lymphoma targeting aptamer (C10.36) were incubated with Ramos cells, a non-Hodgkin lymphoma cell line. (rsc.org)
  • ATW0026 Aptamer to Human Latency Associated Peptide TGF-beta is secreted by many cell types, including macrophages, in a latent form in which it is complexed with two other polypeptides, latent TGF-beta binding protein (LTBP) and latency associated peptide (LAP) . (basepairbio.com)
  • The peptide aptamer cassettes are useful to, e.g., inhibit protein function such as proteins necessary for the transformation of plants, or to replicate cells. (patentsencyclopedia.com)
  • 1. A peptide aptamer expression cassette comprising a DNA sequence encoding a polyprotein that constrains a peptide aptamer by an autofluorescent protein linked translationally to one end of an aptamer, and a fragment of an autofluorescent protein linked translationally to the other end. (patentsencyclopedia.com)
  • 4. The peptide aptamer cassette of claim 1 wherein the autofluorescent protein linked to the aptamer is mCherry and the fragment linked to the aptamer is nVenus. (patentsencyclopedia.com)
  • 7. A method to detect and identify a target protein, the method comprising interacting the peptide aptamer of claim 1 (within the polyprotein) with the target protein and detecting the resulting fluorescence. (patentsencyclopedia.com)
  • 8. A method to inhibit at least one specific target protein function by binding the peptide aptamer expression cassette of claim 1 to the target protein. (patentsencyclopedia.com)
  • 12. A system comprising use of autofluorescence, BiFC, and peptide aptamers, wherein the system comprises introduction of (an) aptamer expression cassette(s) of claim 1 into a cell, and identification of a target protein or a target phenotype by autofluorescence and BiFC. (patentsencyclopedia.com)
  • 13. The system of claim 12 further comprising recovery and identification of the aptamer responsible for interaction with a target protein or a target phenotype. (patentsencyclopedia.com)
  • [0006] Aptamers can be "free" (i.e., as a "tag" on the end of another protein) or "constrained" (i.e., inserted between two other polypeptides). (patentsencyclopedia.com)
  • The aptamer also specifically bound to the extracellular domain of HER2 protein with a K d of 316 nM. (nature.com)
  • With cell-based selection, a specific protein target is not always chosen, but selection is performed against a target cell type with the goal of letting the aptamer choose the target. (mdpi.com)
  • Herein we directly integrate receptive aptamers into a redox charging peptide support in enabling a label-free low picomolar analytical assay for C-reactive protein with a sensitivity that significantly exceeds that attainable with an analogous antibody interface. (browngroupnucleicacidsresearch.org.uk)
  • We propose that peptide aptamers can provide a very useful and new alternative for interfering with protein-protein interactions in intracellular signal transduction cascades, including those emanating from activated receptors for growth factors. (mdpi.com)
  • Unique quadruplex structure and interaction of an RNA aptamer against bovine prion protein. (nih.gov)
  • RNA aptamers against bovine prion protein (bPrP) were obtained, most of the obtained aptamers being found to contain the r(GGAGGAGGAGGA) (R12) sequence. (nih.gov)
  • This is the first report on the structure of an RNA aptamer against prion protein. (nih.gov)
  • In addition, the aptamer eluted FLAG-tagged proteins from the antibody, suggesting a commercial application in protein purification. (hindawi.com)
  • We present a novel strategy that offers practical solutions to these challenges, featuring peptide aptamers as artificial protein detectors arrayed on gold electrodes with feature sizes one order of magnitude smaller than existing formats. (biomedcentral.com)
  • As a proving proof of principle experiment, we demonstrate the specific recognition of cyclin-dependent protein kinases in whole-cell lysates using arrays of ten electrodes functionalized with individual peptide aptamers, with no measurable cross-talk between electrodes. (biomedcentral.com)
  • The use of peptide aptamers selected in vivo to recognize specific protein isoforms, the ability to functionalize each microelectrode individually, the electronic nature and scalability of the label-free detection and the scalability of the array fabrication combine to yield the potential for highly multiplexed devices with increasingly small detection areas and higher sensitivities that may ultimately allow the simultaneous monitoring of tens or hundreds of thousands of protein isoforms. (biomedcentral.com)
  • They consist of one (or more) short variable peptide domains, attached at both ends to a protein scaffold. (wikipedia.org)
  • We have addressed this using a peptide aptamer that binds to the second LIM domain of the LMO2 protein and disrupts its function. (ox.ac.uk)
  • With the help of the nuclear location signal peptide and AS1411 aptamer with specific affinity for nucleolin in the tumor cell membrane and nuclei, the delivery vector can specifically deliver the plasmid to the nuclei of tumorous cells for knocking out the protein tyrosine kinase 2 (PTK2) gene to down-regulate focal adhesion kinase (FAK). (usda.gov)
  • Uncoupling the protein-protein interaction between collapsin response mediator protein 2 (CRMP2) and N-type voltage-gated calcium channel (CaV2.2) with an allosteric CRMP2-derived peptide (CBD3) is antinociceptive in rodent models of inflammatory and neuropathic pain. (elsevier.com)
  • Microanalytical protein characterization with multidimensional liquid chromatography/mass spectrometry improves the throughput and reliability of peptide mapping. (researchandmarkets.com)
  • Here we characterize the interaction of the DNA aptamer, GBI-10, with tenascin-C, an extracellular protein found in the tumor matrix. (pnas.org)
  • Molecular modeling and SPRi investigations of interleukin 6 (IL6) protein and DNA aptamers). (ncat.edu)
  • Aptamer-protein interacting pairs play a variety of physiological functions and therapeutic potentials in organisms. (biomedcentral.com)
  • Rapidly and effectively predicting aptamer-protein interacting pairs is significant to design aptamers binding to certain interested proteins, which will give insight into understanding mechanisms of aptamer-protein interacting pairs and developing aptamer-based therapies. (biomedcentral.com)
  • In this study, an ensemble method is presented to predict aptamer-protein interacting pairs with hybrid features. (biomedcentral.com)
  • These results suggest that the proposed method can be a potential candidate for aptamer-protein interacting pair prediction, which may contribute to finding novel aptamer-protein interacting pairs and understanding the relationship between aptamers and proteins. (biomedcentral.com)
  • With a deeper understanding of aptamers in terms of their conformational and protein-binding properties, aptamer-protein interacting pairs have a potential to perform a variety of functions [ 8 , 9 ]. (biomedcentral.com)
  • Due to the physiological functions and practical applications of aptamers, designing aptamers binding to certain interested proteins is crucial to gain insight into mechanisms of aptamer-protein interacting pairs and develop aptamer-based therapies for various diseases. (biomedcentral.com)
  • Therefore, it would be of help to develop a computational method for rapidly and effectively predicting the aptamer-protein interacting pairs based on sequence information. (biomedcentral.com)
  • To address these problems, the researchers have now developed two DNA aptamer-based tests - Aptamer Linked Immobilized Sorbent Assay (ALISA) and Electrochemical Sensor (ECS) for detection of a bacterial protein in the sputum. (thehindubusinessline.com)
  • We have successfully developed the first prototype of an electrochemical protein biosensor using polypyrrole as the substrate and doped aptamer as the probe. (ebscohost.com)
  • Rapid Development of New Protein Biosensors Utilizing Peptides Obtained via Phage Display. (ebscohost.com)
  • Microfabricated high-performance microwave impedance biosensors for detection of aptamer-protein interactions. (ebscohost.com)
  • High-frequency impedance biosensors with nanometer gaps have been prepared for the detection of biomolecular interactions such as protein-antibody and protein-aptamer binding. (ebscohost.com)
  • Moreover, AGEs-aptamer significantly reduced gene expression of RAGE , monocyte chemoattractant protein-1, connective tissue growth factor, and type IV collagen both in the kidney of KKAy/Ta mice and in AGE-exposed human cultured mesangial cells. (diabetesjournals.org)
  • In contrast, peptide based aptamers consist of a variable loop region that is attached on both ends to a large scaffold protein for solubility. (antibodies-online.com)
  • Traditionally, a randomized peptide expression library is displayed by the E. coli thioredoxin A (TrxA) protein. (antibodies-online.com)
  • Slow Off-rate Modified Aptamers (SOMAmers 1 ) are a particular class of aptamers that consist of a short DNA sequence that incorporate several modified bases with protein-like side chains. (antibodies-online.com)
  • In another aptamer-based dot blot, conjugation of aptamers to gold nanoparticles enabled visualization of a protein target without instrumentation. (cambio.co.uk)
  • The peptide aptamers are designed to restrict other protein interactions inside the cells. (epicos.com)
  • Peptides could offer a direct interaction by affinity selection against viral proteins involved in infection cycle, like capsid or movement protein (e.g.) and affect viral replication. (scirp.org)
  • Here, we present a brief survey of the use of aptamers in signaling pathways, in particular of polypeptide growth factors, starting with the published as well as potential applications of aptamers targeting Epidermal Growth Factor Receptor signaling. (mdpi.com)
  • Peptide amphiphile micelles (PAMs) are a powerful platform technology for improving the delivery of therapeutic and prophylactic peptides. (rsc.org)
  • C10.36∼A/PAMs were found not only to be stable in blood-like conditions, but also to be capable of facilitating delivery of therapeutic Tat-POSH peptide to Ramos cells in vitro . (rsc.org)
  • In order to enhance the therapeutic efficacy of the aptamer-siRNA, we increased the valency of the construct by rational design. (eurekaselect.com)
  • The present invention is directed to a therapeutic composition in which human PDGF is bound directly to peptides that self assemble into a biologically compatible gel. (google.com)
  • The binding aptamers can be attached to a variety of therapeutic compounds for targeted drug delivery. (onenucleus.com)
  • What's more, aptamers have a potential in cancer diagnosis and developing target-based therapeutic drugs delivery to cancer cells, which can reduce side effects of most chemotherapeutic drugs [ 12 ]. (biomedcentral.com)
  • December 2017: The Caris Life Sciences identified new mechanism of action for an aptamer with therapeutic potential to treat non-Hodgkin lymphoma. (prsync.com)
  • The dual-function aptamer avoided another problem often found with therapeutic RNAs: No interferon responses were generated, indicating that the chimera was not causing an immune reaction. (sciencemag.org)
  • Iso lated aptamers may directly inhibit the function of target proteins, or they can also be formulated for use as delivery agents for other therapeutic or imaging cargoes. (scirp.org)
  • This review focuses on recent advances in the use of nucleic acid aptamers as diagnostic and therapeutic agents and as targeted delivery carriers that are relevant to lymphoma. (scirp.org)
  • K. Shum, J. Zhou and J. Rossi, "Nucleic Acid Aptamers as Potential Therapeutic and Diagnostic Agents for Lymphoma," Journal of Cancer Therapy , Vol. 4 No. 4, 2013, pp. 872-890. (scirp.org)
  • J. Zhou and J. J. Rossi, "The Therapeutic Potential of Cell-Internalizing Aptamers," Current topics in Medicinal Chemistry, Vol. 9. (scirp.org)
  • He and others have applied these PSMA-targeting aptamers to deliver various therapeutic and imaging packages to prostate cancer cells and tumors. (hopkinsmedicine.org)
  • Our present data suggest that continuous administration of AGEs-aptamer could protect against experimental diabetic nephropathy by blocking the AGEs-RAGE axis and may be a feasible and promising therapeutic strategy for the treatment of diabetic nephropathy. (diabetesjournals.org)
  • Therapeutic and analytical applications of DNA G-quadruplex aptamers are also discussed. (eurekaselect.com)
  • One method being developed is to tether short (typically 25-nucleotide) single-stranded deoxyribo (DNA) or peptide (PNA) nucleic acid chains to the gate area of the transistor to act as bait for capturing nucleic acids with the complementary sequence. (umd.edu)
  • While previous research has shown aptamer-displaying PAMs enhance cell association, transportation to intracellular targets still remains a substantial hurdle for these biomaterials. (rsc.org)
  • In the capture assay applications, aptamers are applied for the purification of their corresponding targets for a wide variety of purposes [ 11 , 27 , 28 ]. (hindawi.com)
  • Both DNA and RNA aptamers show robust binding affinities for various targets. (wikipedia.org)
  • Ulrich Wullner, Inga Neef, Andreas Eller, Michael Kleines, Mehmet Kemal Tur and Stefan Barth, " Cell-Specific Induction of Apoptosis by Rationally Designed Bivalent Aptamer-siRNA Transcripts Silencing Eukaryotic Elongation Factor 2", Current Cancer Drug Targets (2008) 8: 554. (eurekaselect.com)
  • Figure 1 depicts the structures of two aptamers binding to specific targets. (biomedcentral.com)
  • Aptamers antagonise their lncRNA targets by blocking the interactions between lncRNAs and critical factors. (els.net)
  • Highly specific and tight-binding nucleic acid aptamers have been selected against a variety of molecular targets for over 20 years. (eurekaselect.com)
  • We compare the diversity of DNA G-quadruplex aptamers selected against multiple ligand targets, and consider structure with a particular focus on dissecting the thrombin binding aptamer - thrombin interaction. (eurekaselect.com)
  • Administration of TAP siRNA conjugated to a broad-range tumor-targeting nucleolin aptamer inhibited tumor growth in multiple tumor models without measurable toxicity, was comparatively effective to vaccination against prototypic mutation-generated neoantigens, potentiated the antitumor effect of PD-1 antibody or Flt3 ligand, and induced the presentation of a TAP-independent peptide in human tumor cells. (nature.com)
  • A new method of labeling peptides and proteins with F-18 via a metal ligand" Abstract #384, J Nucl Med. (freepatentsonline.com)
  • A new method of labeling peptides and proteins with F-18 via a metal ligand", Power Point Presentation, 55th SNM Annual Meeting, New Orleans, LA, Jun. (freepatentsonline.com)
  • A New Method of Labeling Peptides with F-18 Via a Metal Ligand" Abstract #68, 19th Winter Fluorine Conference (Jan. 11-16, 2009) Abstract Book, p. 32. (freepatentsonline.com)
  • A New Method of Labeling Peptides with F-18 Via a Metal Ligand" PowerPoint Presentation, 19th Winter Fluorine Conference, St. Pete Beach, FL, Jan. 13, 2009. (freepatentsonline.com)
  • Demands for new diagnostic assay and increasing research activity in emerging markets such as China and Japan is likely to boost growth of Asia Pacific aptamer market at a CAGR of around 21.0% from 2017 to 2025. (openpr.com)
  • Analogous to the point-of-care diagnostics, there is no doubt that aptamers can also be deployed in the point-of-care aptamer-based decontamination assay, whereby decontamination can be performed anywhere and anytime for instantaneous decision-making. (hindawi.com)
  • Apart from biosensing application, aptamers have also been the subject in the aptamer-based capture assay. (hindawi.com)
  • The present review seeks to present an overview of the more narrowed context of the aptamer-based capture assay, which is on the usage of the aptamer in the decontaminating process or simply abbreviated as apta-decontamination assay. (hindawi.com)
  • Several aspects of the apta-decontamination assay will be deliberated, which encompasses the functionalization of the aptamer to the usage of the aptamer in decontaminating environment, food sample, and in in vivo application. (hindawi.com)
  • Reusability of the aptamer and the development of point-of-care apta-decontamination assays are explored before envisaging the potential future trend of the apta-decontamination assay. (hindawi.com)
  • The peptide inhibits Lmo2 function in a mouse T-cell tumor transplantation assay by preventing Lmo2-dependent T-cell neoplasia. (ox.ac.uk)
  • An aptamer-based dot blot assay is more robust and can be designed for point-of-care clinical testing and on-site environmental and industrial analysis. (cambio.co.uk)
  • While antibody-based immunoassays have proven to be very sensitive and specific, there are some limitations which can be overcome with the ELASA, or Enzyme-Linked Aptamer Sorbent Assay. (cambio.co.uk)
  • However, the diagnostics segment accounted for the 30% share of the global aptamer market in 2016. (transparencymarketresearch.com)
  • As key players introduce their new aptamer services for different application in developed markets such a North America and Western Europe, these regions are likely to account for dominating share of the global aptamer market. (openpr.com)
  • One peptide (WGQWPYHC) revealed specific cytotoxicity according to the TCTP expression in tumor cells without affecting normal cells. (springer.com)
  • Treatment with the chemically-synthesized nucleolin aptamer-TAP siRNA conjugate represents a broadly-applicable approach to increase the antigenicity of tumor lesions and thereby enhance the effectiveness of immune potentiating therapies. (nature.com)
  • With the aim of developing a clinically translatable and broadly applicable approach to induce TAP deficiency-induced neoantigens in tumor cells in vivo, we used siRNA to downregulate TAP that was targeted to murine and human tumor cells by conjugation to a broad-range nucleolin-binding oligonucleotide aptamer. (nature.com)
  • One possible approach is the use of aptamers to deliver siRNAs selectively to tumor cells with appropriate antigens displayed on the surface. (eurekaselect.com)
  • We have probed antigens presented by a monolayer of tumor cells for their ability to interact with a pool of aptamers. (pnas.org)
  • Prostate-targeted radiosensitization via aptamer-shRNA chimeras in human tumor xenografts. (hopkinsmedicine.org)
  • 11. A method to generate a phenotype, the method comprising using the peptide expression cassette of claim 1 and using target independent assays to detect the phenotype. (patentsencyclopedia.com)
  • Research applications are specifically discussed regarding aptamers use in flow cytometry, reagents and assays, mass spectrometry and western blotting. (bio-medicine.org)
  • Specifically, the colour change caused by their aggregation or disaggregation and connected to the aptamer-target binding reaction has been used in the development of assays and tests. (smw.ch)
  • They are particular suitable for quantum dot-stimulated FRET-based (beacon) aptamer assays [61]. (smw.ch)
  • The two-year agreement will give AstraZeneca use of Aptamer Group's proprietary technology to explore the feasibility of developing the next generation of drug delivery vehicles, called Aptamer Drug Conjugates (ApDCs). (onenucleus.com)
  • Because of their target affinity, aptamers can direct the transport of aptamer-nanoparticle conjugates. (smw.ch)
  • The binding of the aptamers to the target "anchors" the nanoparticle-aptamer conjugates at their site of action. (smw.ch)
  • Transparency Market Research, a leading U.S based market research firm analyses the global market for aptamers and estimates the CAGR of 73.1% for the period 2013 - 2019. (mynewsdesk.com)
  • Global Aptamers Market Technology Trend Analysis Market Report 2013-2018: Therapeutics, Diagnostics, Biosensors, Biomarker/Drug Discovery & Ap. (bio-medicine.org)
  • Middle East and Africa Aptamers Market by type - (Peptide aptamers, Nucleic Acid aptamers (DNA-Based Aptamers, RNA-Based Aptamers, and XNA-Based Aptamers)), by Application - (Therapeutics Development, Diagnostics, Research and Development), by End users. (emailwire.com)
  • Aptamer technology applies in number of areas such as diagnostics, food safety, research applications and therapeutics in number of industrial sectors like pharmaceutical, research laboratories and biotechnology. (mynewsdesk.com)
  • The results presented here demonstrate the usefulness of multivalent aptamer-based delivery vehicles for siRNA therapeutics. (eurekaselect.com)
  • The launch of Macugen, the first aptamer drug and first diagnostic test for testing mycotoxins in grains has acknowledged the potential of aptamers in diagnostics and therapeutics. (prsync.com)
  • Understanding DNA G-quadruplex aptamers carries implications not only for therapeutics and diagnostics, but also in the natural biochemistry of guanine-rich nucleic acids. (eurekaselect.com)
  • The report suggests that surge in interest of research activity in the field of aptamer technology is likely to spur the demand of aptamer in the coming years (2017 to 2025). (openpr.com)
  • Diagnostics segment, accounted for more than 30.0% share of global aptamer market in 2016, in terms of value. (openpr.com)
  • This review article gives a brief overview of recent relevant research into aptamers and trends in their use in cancer diagnostics and therapy. (smw.ch)
  • The main part of the article is dedicated to current developments of aptamer-modified nanoparticles and their use in cancer diagnostics and treatment. (smw.ch)
  • Peptide Aptamers-Endothelial Cells (P36), Peptide Aptamer, FITC labelled-Genemed Synthesis Inc. (genemedsyn.com)
  • s r.o. offers the synthesis of peptides with the chain length from 2 to 40 amino acid moieties in demanded purity. (cebiotech.com)
  • For the peptide synthesis the recent technologies in the art are used, like automatic peptide synthesizer, microwave device and modern HPLC systems. (cebiotech.com)
  • Microarrays designed for RNA aptamer screening and binding optimization and built on the flexible and powerful Paraflo microfluidic on-chip synthesis platform. (bio-medicine.org)
  • So, the Middle-East and African market for aptamers is expected to grow, which has been backed by advancements in technology with respect to selection processes and synthesis. (emailwire.com)
  • Other features of aptamers like favorable toxicological property, high stability, ease of synthesis and rapid tissue penetration would significantly contribute to the growth of the market. (mynewsdesk.com)
  • So, the global market for aptamers is expected to raise, backed by technological advancements with respect to selection processes and synthesis. (prsync.com)
  • Chemical aptamer synthesis enables rapid, low-cost production of new batches with low lot-to-lot variability. (cambio.co.uk)
  • We report the protocols for the synthesis and purification of Peptide Nucleic Acid (PNA) oligomers incorporating modified residues. (jove.com)
  • PNAs can be synthesized relatively easily by the manual Boc-chemistry solid-phase peptide synthesis method. (jove.com)
  • Several unique properties of aptamers make them attractive tools for use in a wide array of molecular biology applications and, moreover, as potential pharmaceutical agents. (mdpi.com)
  • 1. A drug delivery composition which is suitable for transmucosal delivery and which comprises a plurality of microspheres adapted to gel in contact with the mucosal surface and active drug associated with each microsphere, the composition being substantially free of an enhancer, characterised in that the drug is for systemic delivery and is a peptide having a maximum molecular weight of 6000, and the microspheres comprise starch, gelatin, collagen or dextran. (google.es)
  • 9. A drug delivery composition according to claim 1 wherein the peptide has a molecular weight of at least 1000. (google.es)
  • Membrane partitioning of peptide aggregates: coarse-grained molecular dynamics simulations. (nih.gov)
  • By covering the full spectrum of topics relevant to peptide drugs, this timely handbook serves as an introductory reference for both drug developers and biomedical researchers interested in pharmaceutically active peptides, presenting both the advantages and challenges associated with this molecular class. (researchandmarkets.com)
  • Downstream-peptide RNAs are found upstream of short open reading frames (ORFs) that are predicted to encode short peptides (usually between 17 and 100 amino acids). (wikipedia.org)
  • It was hypothesized that Downstream-peptide RNAs correspond to riboswitches, based on multiple lines of evidence. (wikipedia.org)
  • This, and other evidence, suggests that glnA RNAs are riboswitches, and their structural similarity to Downstream-peptide RNAs in turn suggests that Downstream-peptide RNAs are also riboswitches. (wikipedia.org)
  • Downstream-peptide RNAs overlap a predicted non-coding RNA called yfr6 that is over 200 nucleotides in length, but it was proposed that only the upstream region (corresponding to the Downstream-peptide motif) functions as an RNA structure. (wikipedia.org)
  • A distinct predicted non-coding RNA called yfr14 overlaps both yfr6 and Downstream-peptide RNAs. (wikipedia.org)
  • Your goal will be to explore what experimental parameters affect the enrichment of a heme-binding RNA aptamer from a mixture of heme-binding and non-binding RNAs. (mit.edu)
  • [0005] Peptide aptamers are short peptides of random amino acid sequences. (patentsencyclopedia.com)
  • A 37-amino acid peptide derived from the calcitonin gene. (jove.com)
  • They can additionally include organic dyes for optical detection, just as functional groups for their conjugation with aptamers [52, 61]. (smw.ch)
  • EXPRESS] Primary sensory neuron-specific interference of TRPV1 signaling by AAV-encoded TRPV1 peptide aptamer attenuates neuropathic pain. (painresearchforum.org)
  • AAV-encoded CaV2.2 peptide aptamer CBD3A6K for primary sensory neuron-targeted treatment of established neuropathic pain. (mcw.edu)
  • A peptide aptamer library was screened using an anti-HPA-1a human monoclonal antibody as a bait to isolate an aptamer that mimics the human platelet antigen HPA-1a. (haematologica.org)
  • The DNA aptamer and FLAG peptide competed for binding to the antigen-binding pocket of the M2 antibody. (hindawi.com)
  • In some cases, the peptide aptamer competitively associated with the antinuclear autoantibodies, thereby preventing antibody-mediated tissue damage [ 10 , 11 ]. (hindawi.com)
  • Aptabodies are formed by hybridization of two DNA aptamers and allow detection of human thrombin effectively compared to traditional aptamers. (openpr.com)
  • Aptamers are generated quickly and applied in the inhibition, characterization of proteins and for particular detection. (prsync.com)
  • We aim to develop aptamers for detection of multiple bacterial proteins simultaneously which is expected to lead a more robust test," said Tarun Sharma, a member of the research team. (thehindubusinessline.com)
  • The group used aptamer-based test also for detection of pleural TB, the second most prevalent form of extrapulmonary TB. (thehindubusinessline.com)
  • The huge potential of aptamer-nanoparticle-based detection of cancer cells and biomedical in - vivo imaging has been shown, with some examples. (smw.ch)
  • Aptamer-modified nanoparticles for medical applications are developed mostly for the detection of analytes, for biomedical in-vivo imaging and for in-vivo drug delivering. (smw.ch)
  • From purification to imaging to detection/quantification, aptamers are enabling the use of traditional methods in new and different ways. (cambio.co.uk)
  • Biotinylated aptamer was used for detection along with a streptavidin-HRP / TMB enzyme-substrate system. (cambio.co.uk)
  • Following incubation with sample and appropriate wash steps, bound analyte is often detected with a FITC-labelled detection aptamer and the use of an anti-FITC-HRP / TMB enzyme-substrate system for colorimetric detection. (cambio.co.uk)
  • Furthermore, presence of several key players such as Aptagen, Inc., Regado Biosciences, Inc. and Somalogic, Inc. has resulted in increased investment in research and development of novel aptamers. (openpr.com)
  • The aim of this project is to increase the interaction of nano hydroxyapatite and titanium oxide using an organic interphase of peptides known as aptamers. (open.ac.uk)
  • It describes aptamers that are selected with pre-defined equilibrium ( K d {\displaystyle K_{\mathrm {d} }} ), rate ( k o f f {\displaystyle k_{\mathrm {off} }} , k o n {\displaystyle k_{\mathrm {on} }} ) constants and thermodynamic (ΔH, ΔS) parameters of aptamer-target interaction. (wikipedia.org)
  • A homopolyarginine (R9)-conjugated CBD3-A6K (R9-CBD3-A6K) peptide inhibited the CaV2.2-CRMP2 interaction in a concentration-dependent fashion and diminished surface expression of CaV2.2 and depolarization-evoked Ca 2+ influx in rat dorsal root ganglia neurons. (elsevier.com)
  • Intracellular Analysis of the Interaction between the Human Papillomavirus Type 16 E6 Oncoprotein and Inhibitory Peptides. (nih.gov)
  • Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. (springer.com)
  • Rivillas-Acevedo, L.A. and Soriano-García, M. (2007) Isolation and Biochemical Characterization of an Antifungal Peptide from Amaranthus hypochondriacus Seeds. (scirp.org)
  • Western blot analysis showed peptide-induced down-regulation of TCTP as primary target as well as of cell-cycle related downstream proteins (CDK2, CDK6, Cyclin D3) in MOLT-4 leukemia cells. (springer.com)
  • One of these is a potent inhibitor of E2F binding activity in vitro and in mammalian fibroblasts, blocks cells in G1, and the free variable region from this aptamer has the same effect. (nih.gov)
  • 6. A plasmid "backbone" capable of replicating the aptamer expression cassette of claim 1 in bacterial, yeast, fungal, animal, or plant cells. (patentsencyclopedia.com)
  • Collectively, a newly developed DNA aptamer (HB5) was found to be capable of specifically binding to HER2-positive breast cancer cells. (nature.com)
  • We used an aptamer that binds specifically to PSMA, a cell surface glycoprotein found in abundance on prostate cancer cells, and joined its 3 end to a siRNA specific for Eukaryotic Elongation Factor 2 mRNA (EEF2). (eurekaselect.com)
  • The development of more aptamers specific to cancer cells or marker proteins is urgently needed, especially for these applications. (smw.ch)
  • Mandal, S.M., Migliolo, L. and Franco, O.L. (2012) The Use of MALDI-TOF-MS and in Silico Studies for Determination of Antimicrobial Peptides' Affinity to Bacterial Cells. (scirp.org)
  • Base Pair gives custom aptamer discovery and development services, as well as a line of catalog aptamers. (prsync.com)
  • see the description tab (below) for details of our custom aptamer service. (cambio.co.uk)
  • The hemin, thrombin, and interferon γ, DNA and RNA aptamers were selected through independent selections and have unique sequences. (wikipedia.org)
  • Major factors responsible for the dominance of this segment are efforts by several key players to develop new aptamers for research and development applications. (openpr.com)
  • Thus, these types of collaboration will help companies to develop new aptamers hence drives the market growth. (mynewsdesk.com)
  • In Nucleic Acid and Peptide Aptamers: Methods and Protocols , expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. (springer.com)
  • Cutting-edge and easy to use, Nucleic Acid and Peptide Aptamers: Methods and Protocols will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues. (springer.com)
  • Peptide aptamers and the methods to produce cassettes including the aptamers and manipulating them, are described. (patentsencyclopedia.com)
  • Obviously, it is time-consuming and costly to design aptamers for specific proteins using experimental methods. (biomedcentral.com)
  • Methods for aptamer-based nanoparticle application have been developed, but mostly by use of a few aptamers. (smw.ch)
  • A brief overview of aptamer use and advantages for just a few of the many potential aptamer methods is provided below. (cambio.co.uk)
  • Aptamer performance has been demonstrated in a number of different methods. (cambio.co.uk)
  • Several of the many methods being re-developed with aptamers are listed here. (cambio.co.uk)
  • In this paper we review current aspects in the use of peptides and peptidomics as a strategy to study new methods that lead a direct control against plant viral diseases. (scirp.org)
  • We first confirmed that the AS1411 nucleolin aptamer used in our study 17 conjugated to a TAP siRNA (Supplementary Fig. 1a ) accumulated preferentially in the subcutaneously growing 4T1 breast carcinoma tumors (Supplementary Fig. 1b ). (nature.com)
  • Increasing research activities for development of the novel aptamers to be used in numerous medical application is augmenting adoption and fuelling the growth of the global aptamer market. (transparencymarketresearch.com)
  • Aptamers are usually created by selecting them from a large random sequence pool, but natural aptamers also exist in riboswitches. (prezi.com)
  • For some applications, aptamers offer clear advantages. (cambio.co.uk)
  • Chemically modified Peptide Nucleic Acid (PNA) oligomers have been recently developed that can recognize RNA duplexes in a sequence-specific manner. (jove.com)
  • PNAs are chemically stable with a neutral peptide-like backbone. (jove.com)
  • However, the achievement of Macugen has given a boost to the aptamer field. (bio-medicine.org)
  • Moreover, large number companies have increased their research expenses on aptamers after first aptamer- Macugen approved by USFDA in 2004. (mynewsdesk.com)
  • 3. The peptide aptamer expression cassette of claim 1, further comprising a transcriptional terminator signal sequence. (patentsencyclopedia.com)
  • This length provides enough flexibility for the peptide to assume various conformations, while reducing the probability of randomly creating a stop codon in the aptamer coding sequence. (patentsencyclopedia.com)
  • 14. The system of claim 1, where the aptamer comprises a polynucleotide having a sequence selected from the group consisting of SEQ ID NOS. (freepatentsonline.com)
  • Considering that not all DNA analogs of RNA aptamers show functionality, the correlation between DNA and RNA sequence and their structure and function requires further investigation. (wikipedia.org)
  • Two anti-PSMA aptamers were designed such that each binding sequence could fold independently into its active conformation. (eurekaselect.com)
  • Here, we use a two hybrid approach to isolate from combinatorial libraries peptide aptamers that specifically interact with E2Fs DNA binding and dimerization domains. (nih.gov)
  • and second particles coupled to a second oligonucleotide, the second oligonucleotide complementary to at least a portion of the aptamer. (freepatentsonline.com)
  • 8. The system of claim 5, where the hybridization stability of the aptamer in combination with the analyte is greater than the hybridization stability of a portion of the aptamer with the second oligonucleotide. (freepatentsonline.com)
  • However, the region has not developed any oligonucleotide specific guideline that can address the general requirements for the quality of aptamer clinical trials. (openpr.com)
  • The conserved sites G 133 K 134 and D 138 K 139 R 140 of C-terminal SmpB were identified by interacting with N-terminal SN, and concurrently the residue K 62 of ArfA was recognized by interacting with the surface loop of the specific peptide aptamer PA-2. (frontiersin.org)
  • We describe a method to immobilize specific peptide aptamers on individual electrodes at the micrometer scale, together with a robust and label-free electronic sensing system. (biomedcentral.com)
  • The features for aptamers are extracted from Pseudo K-tuple Nucleotide Composition (PseKNC) while the features for proteins incorporate Discrete Cosine Transformation (DCT), disorder information, and bi-gram Position Specific Scoring Matrix (PSSM). (biomedcentral.com)
  • Aptamers can be combined with ribozymes to self-cleave in the presence of their target molecule. (wikipedia.org)
  • DNA and RNA aptamers have been selected for the same target. (wikipedia.org)
  • Aptamer Group today announced it has signed an agreement with AstraZeneca to explore the potential of using aptamer technology to target the kidney. (onenucleus.com)