Aptamers, Nucleotide
Aptamers, Peptide
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Peptide Library
Peptide Fragments
Antimicrobial Cationic Peptides
Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane.
Base Sequence
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Peptides, Cyclic
Nucleic Acid Conformation
Protein Binding
Peptide Mapping
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
RNA
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Amino Acid Sequence
Binding Sites
Oligonucleotides
Surface Plasmon Resonance
A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.
Oligoribonucleotides
Directed Molecular Evolution
The techniques used to produce molecules exhibiting properties that conform to the demands of the experimenter. These techniques combine methods of generating structural changes with methods of selection. They are also used to examine proposed mechanisms of evolution under in vitro selection conditions.
Natriuretic Peptide, Brain
Biosensing Techniques
Models, Molecular
Vasoactive Intestinal Peptide
Combinatorial Chemistry Techniques
Calcitonin Gene-Related Peptide
Calcitonin gene-related peptide. A 37-amino acid peptide derived from the calcitonin gene. It occurs as a result of alternative processing of mRNA from the calcitonin gene. The neuropeptide is widely distributed in neural tissue of the brain, gut, perivascular nerves, and other tissue. The peptide produces multiple biological effects and has both circulatory and neurotransmitter modes of action. In particular, it is a potent endogenous vasodilator.
Streptavidin
Cell-Penetrating Peptides
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Peptide Biosynthesis
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Protein Structure, Secondary
Gene Library
Protein Footprinting
A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. Protein footprinting utilizes a protein cutting reagent or protease. Protein cleavage is inhibited where the proteins, or nucleic acids and protein, contact each other. After completion of the cutting reaction, the remaining peptide fragments are analyzed by electrophoresis.
Peptide YY
Peptide Nucleic Acids
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
Binding, Competitive
Natriuretic Peptide, C-Type
A PEPTIDE of 22 amino acids, derived mainly from cells of VASCULAR ENDOTHELIUM. It is also found in the BRAIN, major endocrine glands, and other tissues. It shares structural homology with ATRIAL NATRIURETIC FACTOR. It has vasorelaxant activity thus is important in the regulation of vascular tone and blood flow. Several high molecular weight forms containing the 22 amino acids have been identified.
Natriuretic Peptides
G-Quadruplexes
Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15)
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Structure-Activity Relationship
Gastrin-Releasing Peptide
Ligands
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
Substrate Specificity
Receptors, Formyl Peptide
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
Peptide PHI
A 27-amino acid peptide with histidine at the N-terminal and isoleucine amide at the C-terminal. The exact amino acid composition of the peptide is species dependent. The peptide is secreted in the intestine, but is found in the nervous system, many organs, and in the majority of peripheral tissues. It has a wide range of biological actions, affecting the cardiovascular, gastrointestinal, respiratory, and central nervous systems.
Peptide Synthases
Cloning, Molecular
Peptide Hydrolases
Receptors, Peptide
Chromatography, High Pressure Liquid
Atrial Natriuretic Factor
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
DNA, Single-Stranded
Nucleic Acids
Mass Spectrometry
Drug Delivery Systems
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Riboswitch
Part of a MESSENGER RNA molecule that undergoes a conformation change upon binding a specific metabolite or other small molecule thereby regulating the messenger RNA's transcription, post-transcriptional processing, transport, translation, or stability in response to varying levels of the metabolite or other small molecule.
Trypsin
DNA Primers
Sequence Homology, Amino Acid
Drug Design
The molecular designing of drugs for specific purposes (such as DNA-binding, enzyme inhibition, anti-cancer efficacy, etc.) based on knowledge of molecular properties such as activity of functional groups, molecular geometry, and electronic structure, and also on information cataloged on analogous molecules. Drug design is generally computer-assisted molecular modeling and does not include pharmacokinetics, dosage analysis, or drug administration analysis.
Molecular Probes
Amino Acids
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Molecular Probe Techniques
Cattle
Opioid Peptides
The endogenous peptides with opiate-like activity. The three major classes currently recognized are the ENKEPHALINS, the DYNORPHINS, and the ENDORPHINS. Each of these families derives from different precursors, proenkephalin, prodynorphin, and PRO-OPIOMELANOCORTIN, respectively. There are also at least three classes of OPIOID RECEPTORS, but the peptide families do not map to the receptors in a simple way.
Intracellular Signaling Peptides and Proteins
Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.
Magnetic Resonance Spectroscopy
Peptide Hormones
Nanotechnology
Cells, Cultured
Recombinant Fusion Proteins
Immobilized Proteins
Electrophoresis, Polyacrylamide Gel
Amino Acid Motifs
Amyloid beta-Peptides
Peptides generated from AMYLOID BETA-PEPTIDES PRECURSOR. An amyloid fibrillar form of these peptides is the major component of amyloid plaques found in individuals with Alzheimer's disease and in aged individuals with trisomy 21 (DOWN SYNDROME). The peptide is found predominantly in the nervous system, but there have been reports of its presence in non-neural tissue.
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Electrophoresis, Capillary
Molecular Mimicry
Flexiviridae
Fluorescent Dyes
Microfluidics
Glucagon-Like Peptide 1
A peptide of 36 or 37 amino acids that is derived from PROGLUCAGON and mainly produced by the INTESTINAL L CELLS. GLP-1(1-37 or 1-36) is further N-terminally truncated resulting in GLP-1(7-37) or GLP-1-(7-36) which can be amidated. These GLP-1 peptides are known to enhance glucose-dependent INSULIN release, suppress GLUCAGON release and gastric emptying, lower BLOOD GLUCOSE, and reduce food intake.
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Cell Membrane
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Receptors, Vasoactive Intestinal Peptide
Mutation
Antibodies
Thermodynamics
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
Microfluidic Analytical Techniques
Receptors, Atrial Natriuretic Factor
Epitopes, T-Lymphocyte
Salivary Proteins and Peptides
Chromatography, Affinity
Dose-Response Relationship, Drug
Serine Endopeptidases
Levivirus
Sequence Analysis, RNA
Antigen Presentation
The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)
Peptide aptamers targeting the hepatitis B virus core protein: a new class of molecules with antiviral activity. (1/64)
A substantial proportion of the worldwide liver cancer incidence is associated with chronic hepatitis B virus (HBV) infection. The therapeutic management of HBV infections is still problematic and novel antiviral strategies are urgently required. Using the peptide aptamer screening system, we aimed to isolate new molecules, which can block viral replication by interfering with capsid formation. Eight peptide aptamers were isolated from a randomized expression library, which specifically bound to the HBV core protein under intracellular conditions. One of them, named C1-1, efficiently inhibited viral capsid formation and, consequently, HBV replication and virion production. Hence, C1-1 is a novel model compound for inhibiting HBV replication by blocking capsid formation and provides a new basis for the development of therapeutic molecules with specific antiviral potential against HBV infections. (+info)Identification of the first Rho-GEF inhibitor, TRIPalpha, which targets the RhoA-specific GEF domain of Trio. (2/64)
The Rho-guanine nucleotide exchange factors (Rho-GEFs) remodel the actin cytoskeleton via their Rho-GTPase targets and affect numerous physiological processes such as transformation and cell motility. They are therefore attractive targets to design specific inhibitors that may have therapeutic applications. Trio contains two Rho-GEF domains, GEFD1 and GEFD2, which activate the Rac and RhoA pathways, respectively. Here we have used a genetic screen in yeast to select in vivo peptides coupled to thioredoxin, called aptamers, that could inhibit GEFD2 activity. One aptamer, TRIAPalpha (TRio Inhibitory APtamer), specifically blocks GEFD2-exchange activity on RhoA in vitro. The corresponding peptide sequence, TRIPalpha, inhibits TrioGEFD2-mediated activation of RhoA in intact cells and specifically reverts the neurite retraction phenotype induced by TrioGEFD2 in PC12 cells. Thus TRIPalpha is the first Rho-GEF inhibitor isolated so far, and represents an important step in the design of inhibitors for the expanding family of Rho-GEFs. (+info)The guanine nucleotide exchange factor trio activates the phagocyte NADPH oxidase in the absence of GDP to GTP exchange on Rac. "The emperor's nw clothes". (3/64)
The superoxide-generating NADPH oxidase complex of phagocytes consists of a membrane-associated flavocytochrome b(559) and four cytosolic components as follows: p47(phox), p67(phox), p40(phox), and the small GTPase Rac (1 or 2). Activation of the oxidase is the result of assembly of the cytosolic components with cytochrome b(559) and can be mimicked in vitro by mixtures of membrane and cytosolic components exposed to an anionic amphiphile, serving as activator. We reported that prenylation of Rac1 endows it with the ability to support oxidase activation in conjunction with p67(phox) but in the absence of amphiphile and p47(phox). We now show the following 6 points. 1) The Rac guanine nucleotide exchange factor Trio markedly potentiates oxidase activation by prenylated Rac1-GDP. 2) This occurs in the absence of exogenous GTP or any other source of GTP generation, demonstrating that the effect of Trio does not involve GDP to GTP exchange on Rac1. 3) Trio does not potentiate oxidase activation by prenylated Rac1-GTP, by nonprenylated Rac1-GDP in the presence or absence of amphiphile, and by a prenylated [p67(phox)-Rac1] chimera in GDP-bound form. 4) Rac1 mutants defective in the ability to bind Trio or to respond to Trio by nucleotide exchange fail to respond to Trio by enhanced oxidase activation. 5) A Trio mutant with conserved Rac1-binding ability but lacking nucleotide exchange activity fails to enhance oxidase activation. 6) The effect of Trio is mimicked by displacement of Mg(2+) from Rac1-GDP. These results reveal the existence of a novel mechanism of Rac activation by a guanine nucleotide exchange factor and suggest that the induction by Trio of a conformational change in Rac1, in the absence of nucleotide exchange, is sufficient for enhancing its effector function. (+info)Ultra high-speed sorting. (4/64)
BACKGROUND: Cell sorting has a history dating back approximately 40 years. The main limitation has been that, although flow cytometry is a science, cell sorting has been an art during most of this time. Recent advances in assisting technologies have helped to decrease the amount of expertise necessary to perform sorting. METHODS: Droplet-based sorting is based on a controlled disturbance of a jet stream dependent on surface tension. Sorting yield and purity are highly dependent on stable jet break-off position. System pressures and orifice diameters dictate the number of droplets per second, which is the sort rate limiting step because modern electronics can more than handle the higher cell signal processing rates. RESULTS: Cell sorting still requires considerable expertise. Complex multicolor sorting also requires new and more sophisticated sort decisions, especially when cell subpopulations are rare and need to be extracted from background. High-speed sorting continues to pose major problems in terms of biosafety due to the aerosols generated. CONCLUSIONS: Cell sorting has become more stable and predictable and requires less expertise to operate. However, the problems of aerosol containment continue to make droplet-based cell sorting problematical. Fluid physics and cell viability restraints pose practical limits for high-speed sorting that have almost been reached. Over the next 5 years there may be advances in fluidic switching sorting in lab-on-a-chip microfluidic systems that could not only solve the aerosol and viability problems but also make ultra high-speed sorting possible and practical through massively parallel and exponential staging microfluidic architectures. (+info)T2-TrpRS inhibits preretinal neovascularization and enhances physiological vascular regrowth in OIR as assessed by a new method of quantification. (5/64)
PURPOSE: A carboxyl-terminal fragment of tryptophan tRNA synthetase (T2-TrpRS) has demonstrated potent angiostatic activity during retinal developmental neovascularization in vivo. The effects of T2-TrpRS on pathologic neovascularization were tested and compared with a potent VEGF antagonist using the mouse model of oxygen-induced retinopathy (OIR). METHODS: C57BL/6J mice were transiently exposed to hyperoxic conditions (75% O2) between postnatal day 7 (P7) and P12 and then returned to room air. Retinas were isolated, blood vessels stained with isolectin Griffonia simplicifolia, images of retinal whole-mounts acquired, and the area of vascular obliteration and extent of preretinal neovascularization quantified. This method was compared to the commonly used method of OIR quantification in which the number of pre-inner limiting membrane (ILM) nuclei is counted in serial sections of whole eyes. To assess the angiostatic activity of T2-TrpRS, mice were injected intravitreally at P12 with either T2-TrpRS, a VEGF aptamer, or vehicle (PBS) alone, and the effects on area of obliteration and on preretinal neovascular tuft formation were assessed. RESULTS: Using a modified method of quantification in the mouse OIR model based on images of isolectin-stained retinal wholemounts, we were able to assess reliably and consistently both vascular obliteration and preretinal neovascular tuft formation in the same specimen. T2-TrpRS demonstrated potent angiostatic activity, reducing the appearance of pathologic neovascular tufts by up to 90%. Surprisingly, T2-TrpRS also enhanced physiological revascularization of the obliterated retinal vasculature, reducing these areas by up to 60% compared with PBS-injected eyes. In contrast, the VEGF antagonist, while similarly reducing preretinal neovascular tuft formation, did not enhance revascularization of the obliterated areas. CONCLUSIONS: Use of a rapid, quantifiable method to assess the effect of T2-TrpRS on retinal angiogenesis in the OIR model demonstrates the importance of a quantification system that permits simultaneous analysis of a drug's effect on vascular obliteration as well as on preretinal neovascularization. The results obtained using this method suggest enhanced clinical value for compounds such as T2-TrpRS that not only inhibit pathologic neovascularization, but also facilitate physiological revascularization of ischemic tissue. (+info)Peptide aptamer-mediated inhibition of target proteins by sequestration into aggresomes. (6/64)
Peptide aptamers (PAs) can be employed to block the intracellular function of target proteins. Little is known about the mechanism of PA-mediated protein inhibition. Here, we generated PAs that specifically bound to the duck hepatitis B virus (HBV) core protein. Among them, PA34 strongly blocked duck HBV replication by inhibiting viral capsid formation. We found that PA34 led to a dramatic intracellular redistribution of its target protein into perinuclear inclusion bodies, which exhibit the typical characteristics of aggresomes. As a result, the core protein is efficiently removed from the viral life cycle. Corresponding findings were obtained for bioactive PAs that bind to the HBV core protein or to the human papillomavirus-16 (HPV16) E6 protein, respectively. The observation that PAs induce the specific sequestration of bound proteins into aggresomes defines a novel mechanism as to how this new class of intracellular inhibitors blocks the function of their target proteins. (+info)Peptide aptamers that bind to a geminivirus replication protein interfere with viral replication in plant cells. (7/64)
The AL1 protein of tomato golden mosaic virus (TGMV), a member of the geminivirus family, is essential for viral replication in plants. Its N terminus contains three conserved motifs that mediate origin recognition and DNA cleavage during the initiation of rolling-circle replication. We used the N-terminal domain of TGMV AL1 as bait in a yeast two-hybrid screen of a random peptide aptamer library constrained in the active site of the thioredoxin A (TrxA) gene. The screen selected 88 TrxA peptides that also bind to the full-length TGMV AL1 protein. Plant expression cassettes corresponding to the TrxA peptides and a TGMV A replicon encoding AL1 were cotransfected into tobacco protoplasts, and viral DNA replication was monitored by semiquantitative PCR. In these assays, 31 TrxA peptides negatively impacted TGMV DNA accumulation, reducing viral DNA levels to 13 to 64% of those of the wild type. All of the interfering aptamers also bound to the AL1 protein of cabbage leaf curl virus. A comparison of the 20-mer peptides revealed that their sequences are not random. The alignments detected seven potential binding motifs, five of which are more highly represented among the interfering peptides. One motif was present in 18 peptides, suggesting that these peptides interact with a hot spot in the AL1 N terminus. The peptide aptamers characterized in these studies represent new tools for studying AL1 function and can serve as the basis for the development of crops with broad-based resistance to single-stranded DNA viruses. (+info)Inhibition of transforming growth factor-beta1-induced signaling and epithelial-to-mesenchymal transition by the Smad-binding peptide aptamer Trx-SARA. (8/64)
Overexpression of the inhibitory Smad, Smad7, is used frequently to implicate the Smad pathway in cellular responses to transforming growth factor beta (TGF-beta) signaling; however, Smad7 regulates several other proteins, including Cdc42, p38MAPK, and beta-catenin. We report an alternative approach for more specifically disrupting Smad-dependent signaling using a peptide aptamer, Trx-SARA, which comprises a rigid scaffold, the Escherichia coli thioredoxin A protein (Trx), displaying a constrained 56-amino acid Smad-binding motif from the Smad anchor for receptor activation (SARA) protein. Trx-SARA bound specifically to Smad2 and Smad3 and inhibited both TGF-beta-induced reporter gene expression and epithelial-to-mesenchymal transition in NMuMG murine mammary epithelial cells. In contrast to Smad7, Trx-SARA had no effect on the Smad2 or 3 phosphorylation levels induced by TGF-beta1. Trx-SARA was primarily localized to the nucleus and perturbed the normal cytoplasmic localization of Smad2 and 3 to a nuclear localization in the absence of TGF-beta1, consistent with reduced Smad nuclear export. The key mode of action of Trx-SARA was to reduce the level of Smad2 and Smad3 in complex with Smad4 after TGF-beta1 stimulation, a mechanism of action consistent with the preferential binding of SARA to monomeric Smad protein and Trx-SARA-mediated disruption of active Smad complexes. (+info)cDNA/RNA, ODNs & Kits-Genemed Synthesis Inc.
Peptide Aptamers-Endothelial Cells (P36), Peptide Aptamer, FITC labelled-Genemed Synthesis Inc.
Targeting LMO2 with a peptide aptamer establishes a necessary function in overt T-cell neoplasia. - Radcliffe Department of...
Ultra-high sensitivity of the non-immunological affinity of graphene oxide-peptide-based surface plasmon resonance biosensors...
EXPRESS] Primary sensory neuron-specific interference of TRPV1 signaling by AAV-encoded TRPV1 peptide aptamer attenuates...
IJMS | Free Full-Text | Aptamers and Their Potential to Selectively Target Aspects of EGF, Wnt/β-Catenin and TGFβ-Smad Family...
IJMS | Free Full-Text | Aptamers and Their Potential to Selectively Target Aspects of EGF, Wnt/β-Catenin and TGFβ-Smad Family...
Functionalization of biomedical surfaces by peptide aptamers - Open Research Online
PEPTIDE APTAMERS FOR MANIPULATING PROTEIN FUNCTION - Patent application
A novel assay for the detection of anti-human platelet antigen antibodies (HPA-1a) based on peptide aptamer technology |...
Mallikaratchy Lab | Cell-specific aptamer probes for membrane protein elucidation in cancer cells
A novel aptasensor strategy for protein detection based on G-quadruplex and exonuclease Ⅲ-aided recycling amplification
Applications of aptamers in nanodelivery systems in cancer, eye and inflammatory diseases - DRO
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Productive screening of single aptamers with ddPCR - Analyst (RSC Publishing)
At 19.50% CAGR, Aptamers Market Size, Research, Growth Forecast to surpass USD 7456.8 Million by 2025 - Teletype
Lee S-Y
Developement of maize streak disease (MSD) resistance in select tropical maize inbred lines using peptide aptamiers
The minimal primary structures of RNA aptamers selected to bind HIV-1 reverse transcriptase
Fluorogenic aptamers resolve the flexibility of RNA junctions using orientation-dependent FRET. | NECAT
Plus it
A Toc523240332 h 154.1. Aptamers to neutralize - Free Critical Thinking For Students
http://ufdc.ufl.edu/UFE0025003/00001
Structural determinants of conformationally selective, prion-binding aptamers. - Oxford Neuroscience
LightCycler® 480 RNA Master Hydrolysis Probes
Aptamer Methods
aptamers | Stem Cells Daily
Application of aptamers in histopathology - DRO
Middle East and Africa Aptamers Market Industry Analysis, Size, Share, Growth, Trends, and Forecasts (2016 2021)
A simple non-enzymatic strategy for adenosine triphosphate electrochemical aptasensor using silver nanoparticle-decorated...
SNAP i.d.® 2.0 Protein Detection System Showcased at Neuroscience 2012 | SelectScience
RNA Aptamer Design - De novo Molecular Design - Meyer - Wiley Online Library
In Vitro Selection and Characterization of DNA Aptamers to a Small Molecule Target - Current Protocols
In vitro selection of l-DNA aptamers that bind a structured d-RNA molecule - Texas A&M University (TAMU) Scholar
DNA Aptamers-Adenosine/ATP (DH25.42), DNA Aptamer, unlabeled-Alpha Diagnostic International Inc.
DNA Aptamers-Anti-His Tag (6H7), DNA Aptamer, FITC labeled-Alpha Diagnostic International Inc.
APTAMER - LinkBIO Co. Ltd.
Q-Plex Multiplex Protein Detections - Mbioscience Solutions
Leicester Research Archive: Minisatellite isoalleles can be distinguished by single-stranded conformational polymorphism...
L-Ribonucleic acid aptamer - Wikipedia
Nucleic acid aptamers against biotoxins: A new paradigm toward the treatment and diagnostic approach - Murdoch Research...
A sensitive method to detect Escherichia coli based on immunomagnetic separation and real-time PCR amplification of aptamers<...
Dr. Regina Stoltenburg - Helmholtz-Centre for Environmental Research
Aptamer-Based Analysis and Extraction of Sitosterol and Related Compounds | SBIR.gov
Aptamers 2015 - Library Publishing Media
Non-SELEX: selection of aptamers without intermediate amplification of candidate oligonucleotides | Nature Protocols
PROTEIN DETECTION for MB.BS.pptx - PROTEIN DETECTION Dr.Sajida Parveen Shaikh OBJECTIVES Define proteins List major body...
Biomimetic sensing based on chemically induced assembly of a signaling DNA aptamer on a fluid bilayer membrane
Nucleic Acid Aptamers as a Potential Nucleus Targeted Drug Delivery System<...
SPAD aptasensor for the detection of circulating protein biomarkers<...
Fluorescent Turn-on Aptasensor of Staphylococcus aureus Based on the FRET Between Green Carbon Quantum Dot and Gold...
Aptamers Market driven by increasing interest of research... - Market Research Expert
American Society for Investigative Pathology
Protein Detection using His Tag Antibody and HA Tag Antibody
Protein Detection using His Tag Antibody and HA Tag Antibody
Cellomics Seminar Series 2013: Improving Protein Detection from EMD Millipore Corporation Interdisciplinary Center for...
peroxidase
Selection of Aptamers Against Whole Living Cells: From Cell-SELEX to Identification of Biomarkers | SpringerLink
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Rosave Trio in Kannada ಉಪಯೋಗಗಳು, ಡೋಸೇಜ್, ಅಡ್ಡ ಪರಿಣಾಮಗಳು, ಪ್ರಯೋಜನಗಳು, ಪರಸ್ಪರ ಕ್ರಿಯೆ ಮತ್ತು ಎಚ್ಚರಿಕೆ
Matsusaka Satoshi | TRIOS
Nagata Kyosuke | TRIOS
Downstream-peptide motif
Ames TD, Breaker RR (January 2011). "Bacterial aptamers that selectively bind glutamine". RNA Biol. 8 (1): 82-89. doi:10.4161/ ... Downstream-peptide RNAs are found upstream of short open reading frames (ORFs) that are predicted to encode short peptides ( ... Possible candidates to be regulated by the Downstream-peptide motif are genes that frequently carry the Downstream-peptide ... and their structural similarity to Downstream-peptide RNAs in turn suggests that Downstream-peptide RNAs are also riboswitches ...
Mimotope
... s are a kind of peptide aptamers. When the term mimotope was first coined by Mario Geysen in 1986, it was used to ... It had 10716 peptides grouped into 1229 sets. These peptides were extracted from biopanning results of phage-displayed random ... A mimotope is often a peptide, and mimics the structure of an epitope. Because of this property it causes an antibody response ... However, this concept has been extended to refer peptide mimic of all types of binding sites. As the mimic of binding site, ...
Aptamer
The most common peptide aptamer selection system is the yeast two-hybrid system. Peptide aptamers can also be selected from ... "peptide aptamer." No formal definition exists to distinguish aptamers from non-aptamers, but the typical use is to describe a ... an example of an aptamer against a mushroom target. While most aptamers are based on nucleic acids, peptide aptamers are ... All the peptides panned from combinatorial peptide libraries have been stored in the MimoDB database. Libraries of peptide ...
Affimer
Davis JJ, Tkac J, Humphreys R, Buxton AT, Lee TA, Ko Ferrigno P (May 2009). "Peptide aptamers in label-free protein detection: ... Davis JJ, Tkac J, Laurenson S, Ko Ferrigno P (February 2007). "Peptide aptamers in label-free protein detection: 1. ... Stabilization of the two peptides by the protein scaffold constrains the possible conformations that the peptides can take. ... "Design and validation of a neutral protein scaffold for the presentation of peptide aptamers". Journal of Molecular Biology. ...
RNA interference
... peptides, aptamers, antibodies). Therapeutics using siRNA conjugates have been developed for rare or genetic diseases such as ... Kruspe, S; Giangrande, P (2017). "Aptamer-siRNA Chimeras: Discovery, Progress, and Future Prospects". Biomedicines. 5 (4): 45. ... by RNAi can significantly reduced the amount of Aβ peptide which is correlated with the cause of Alzheimer's disease. In ...
Smart ligand
Known examples of smart ligands include DNA smart aptamers; however, RNA and peptide smart aptamers can also be developed. ... For example, a panel of DNA smart aptamers has been recently used to develop affinity analysis of proteins with ultra-wide ... Baaske P, Wienken CJ, Reineck P, Duhr S, Braun D (Feb 2010). "Optical Thermophoresis quantifies Buffer dependence of Aptamer ... Drabovich AP, Berezovski M, Okhonin V, Krylov SN (May 2006). "Selection of smart aptamers by methods of kinetic capillary ...
L-Ribonucleic acid aptamer
Like other aptamers, L-RNA aptamers are able to bind molecules such as peptides, proteins, and substances of low molecular ... L-RNA aptamers themselves have low antigenicity. In contrast to other aptamers, L-RNA aptamers have high stability in blood ... such as PEGylated L-RNA aptamers, show a prolonged plasma half-life. Unlike other aptamers, L-RNA aptamers are not directly ... L-RNA aptamers are a form of aptamers. Due to their L-nucleotides, they are highly resistant to degradation by nucleases. L-RNA ...
Barrett's esophagus
December 2007). "Microarray-formatted clinical biomarker assay development using peptide aptamers to anterior gradient-2". ...
Nanoparticle
Common address tags are monoclonal antibodies, aptamers, streptavidin or peptides. These targeting agents should ideally be ...
Synthetic antibody
... as in the case of aptamers, or from non-immunoglobulin protein scaffolds / peptide aptamers, into which hypervariable loops are ... 2005). "P. Design and validation of a neutral protein scaffold for the presentation of peptide aptamers". J Mol Biol. 352 (5): ... Within the protein scaffold there exist two variable peptide loops and a variable N-terminal sequence that provide a high ... PMID 24424458.{{cite journal}}: CS1 maint: multiple names: authors list (link) Ladner, R.C. (1995). "Constrained peptides as ...
FOXH1
"Selective inhibition of TGF-beta responsive genes by Smad-interacting peptide aptamers from FoxH1, Lef1 and CBP". Oncogene. 24 ...
SNIP1
"Selective inhibition of TGF-beta responsive genes by Smad-interacting peptide aptamers from FoxH1, Lef1 and CBP". Oncogene. 24 ...
Surface plasmon resonance microscopy
SPR imaging platform would be a good choice to characterize aptamer -protein interactions. To study the aptamer-protein ... One can find protein-protein interaction and on-chip peptide distribution with high spatial resolution using subjected ... The interaction of thrombin and the aptamer can be monitored on microarray in real-time during injections of thrombin at ... Daniel, C; Roupioz, Y; Gasparutti, D; Livache, T; Buhot, A (2013). "Solution-Phase vs Surface-Phase Aptamer-Protein Affinity ...
RNA therapeutics
... homogeneous pool of RNA aptamers can be produced. As such, RNA aptamers can be made to target small peptides and proteins, as ... RNA aptamers can be designed to act as antagonists, agonists, or so-called "RNA decoy aptamers." In the case of antagonists, ... For RNA decoy aptamers, the synthetic RNA aptamer resembles a native RNA molecule. As such, proteins(s) which bind to the ... Additionally, the term "aptamer" was coined by Andrew Ellington, who worked with Jack Szostak to select an RNA aptamer that was ...
Single-walled carbon nanohorn
"Prevention of Carbon Nanohorn Agglomeration Using a Conjugate Composed of Comb-Shaped Polyethylene Glycol and a Peptide Aptamer ... Another example is the specific peptide functionalized SWNHs nanocomposite, which was used to fabricate an immunosensor towards ...
Quantum dot
Antibodies, streptavidin, peptides, DNA, nucleic acid aptamers, or small-molecule ligands can be used to target quantum dots to ... Among these materials are InP/ZnS, CuInS/ZnS, Si, Ge and C. Peptides are being researched as potential quantum dot material. ... Whaley SR, English DS, Hu EL, Barbara PF, Belcher AM (2000). "Selection of peptides with semiconductor binding specificity for ... Hauser, Charlotte A. E.; Zhang, Shuguang (2010). "Peptides as biological semiconductors". Nature. 468 (7323): 516-517. Bibcode: ...
ZFYVE16
... transforming growth factor-beta1-induced signaling and epithelial-to-mesenchymal transition by the Smad-binding peptide aptamer ...
Peptide nucleic acid
... aptamers and ribozymes. In 2015, Jain et al. described a trans-acting DNA-based amphiphatic delivery system for convenient ... Peptide nucleic acid (PNA) is an artificially synthesized polymer similar to DNA or RNA. Synthetic peptide nucleic acid ... Clicked peptide polymer Glycol nucleic acid Oligonucleotide synthesis Peptide synthesis Threose nucleic acid Nielsen PE, Egholm ... Zhao XL, Chen BC, Han JC, Wei L, Pan XB (November 2015). "Delivery of cell-penetrating peptide-peptide nucleic acid conjugates ...
Antibody
They consist of artificial peptides or proteins, or aptamer-based nucleic acid molecules with a molar mass of about 3 to 20 kDa ... Ma B, Zhang K, Hendrie C, Liang C, Li M, Doherty-Kirby A, Lajoie G (2003). "PEAKS: powerful software for peptide de novo ... Reen DJ (1994). "Enzyme-linked immunosorbent assay (ELISA)". Basic Protein and Peptide Protocols. Methods in Molecular Biology ... April 2012). "PEAKS DB: de novo sequencing assisted database search for sensitive and accurate peptide identification". ...
Quyen T. Nguyen
US granted 8685372, Roger Y. Tsien; Quyen T. Nguyen & Michael Whitney, "Peptides and aptamers for targeting of neuron or nerves ... PhD pertaining to their invention of peptides, imaging systems and methods to support fluorescence-guided cancer tumor ...
Intracellular delivery
... aptamers, peptides and antibodies have been covalently linked to siRNA in order to improve cellular uptake and target specific ... Materials that are delivered into cells include nucleic acids (DNA and RNA), proteins, peptides, impermeable small molecules, ... and aptamers. Such oligonucleotides can be used to alter cell behaviour through several different mechanisms . Lipid ... Peptides, and Proteins". Site-Specific Protein Labeling. Methods in Molecular Biology. 1266: 29-53. doi:10.1007/978-1-4939-2272 ...
Riboswitch
... es are often conceptually divided into two parts: an aptamer and an expression platform. The aptamer directly binds ... Two classes of glutamine riboswitches are known: the glnA RNA motif and Downstream-peptide motif. These classes are believed to ... as it contains contiguous dual aptamers. Though no longer shown to be cooperative, the cause of dual aptamers still remains ... of the relevant genes and the success of procedures to create artificial small molecule-binding RNAs called aptamers. In 2002, ...
Hanoch Senderowitz
... and the design of new sustainable pesticides in the form of peptide aptamers and small molecules that would interfere with cell ... 3) Research on computational agriculture including plant disease biocontrol studying anti-bacterial peptides expressed on virus ... Synthetic Cyclic KTS Peptides as Novel Dual Antagonists of α1β1/α2β1 Integrins with Antiangiogenic Activity". In Momic, Tatjana ...
Dose (biochemistry)
DNA aptamers, which are peptides that have with specific target molecules that they search for, fold in response to the ... Electrochemical Aptamer-Based Sensor". Journal of the American Chemical Society. 131 (12): 4262-4266. doi:10.1021/ja806531z. ... Aptamer-Based Tracking of Circulating Therapeutic Agents in Living Animals". Science Translational Medicine. 5 (213): 213ra165 ... this field was initiated with monitoring of small-molecule cocaine levels in undiluted blood serum with electrochemical aptamer ...
Ester H. Segal
... aptamers, and antimicrobial peptides) to enhance the limits of detection for analytes. She has helped engineer hybrid porous ... "Rapid and label-free detection of protein a by aptamer-tethered porous silicon nanostructures". Journal of Biotechnology. ... "Whole-cell detection of live lactobacillus acidophilus on aptamer-decorated porous silicon biosensors". Analyst. 141 (18): 5432 ...
Biointerface
Likewise, Xiao Ma and others, have discussed the electrical control on the binding/unbinding of thrombin from aptamers ... Biointerface science is a multidisciplinary field in which biochemists who synthesize novel classes of biomolecules (peptide ... "Resolving electrical stimulus triggered molecular binding and force modulation upon thrombin-aptamer biointerface". Journal of ... "Electrical Stimulus Controlled Binding/Unbinding of Human Thrombin-Aptamer Complex". Scientific Reports. 6: 37449. Bibcode: ...
Proteomics
These methods offer various advantages, for instance they often are able to determine the sequence of a protein or peptide, ... Jayasena, Sumedha D (1999-09-01). "Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics". Clinical ... One of the earliest methods for protein analysis has been Edman degradation (introduced in 1967) where a single peptide is ... Here, the proteins within a complex mixture are labeled isotopically first, and then digested to yield labeled peptides. The ...
Roger Brent
... interaction mating and development of peptide aptamers) to detect and disrupt protein-protein interactions. In 1997, with ...
Deoxyribozyme
Aptamer - Oligonucleotide or peptide molecules that bind specific targets Ribozyme - Type of RNA molecules Systematic evolution ... Spill F, Weinstein ZB, Irani Shemirani A, Ho N, Desai D, Zaman MH (October 2016). "Controlling uncertainty in aptamer selection ... Travascio P, Li Y, Sen D (September 1998). "DNA-enhanced peroxidase activity of a DNA-aptamer-hemin complex". Chemistry & ... Golub E, Albada HB, Liao WC, Biniuri Y, Willner I (January 2016). "Nucleoapzymes: Hemin/G-Quadruplex DNAzyme-Aptamer Binding ...
Systematic evolution of ligands by exponential enrichment
Aptamer - Oligonucleotide or peptide molecules that bind specific targets Deoxyribozyme - DNA oligonucleotides that can perform ... Aptamers have emerged as a novel category in the field of bioreceptors due to their wide applications ranging from biosensing ... If a good binding affinity for the selected aptamer is not a concern, then an excess of target can be used to increase the ... There are aptamer applications for which a short sequence, and thus primer truncation, is desirable. An advancement on the ...
Digital microfluidics
Jebrail MJ, Ng AH, Rai V, Hili R, Yudin AK, Wheeler AR (November 2010). "Synchronized synthesis of peptide-based macrocycles by ... Superparamagnetic nanoparticles were immobilized with anti-IgE antibodies and fluorescently labeled aptamers to quantify IgE ... Heron SR, Wilson R, Shaffer SA, Goodlett DR, Cooper JM (May 2010). "Surface acoustic wave nebulization of peptides as a ... These include polymerase chain reaction (PCR), as well as the formation of DNA and peptides. Reduction, alkylation, and ...
DNA nanotechnology
While DNA is the dominant material used, structures incorporating other nucleic acids such as RNA and peptide nucleic acid (PNA ... and aptamers, which can bind to specific proteins or small molecules. Structural DNA nanotechnology, sometimes abbreviated as ... emerging applications of DNAzymes and aptamers". Current Opinion in Biotechnology. 17 (6): 580-588. doi:10.1016/j.copbio. ...
Xeno nucleic acid
One experiment conducted at the University of Florida led to the production of an XNA aptamer by the AEGIS-SELEX (artificially ... D'Agata R, Giuffrida MC, Spoto G (November 2017). "Peptide Nucleic Acid-Based Biosensors for Cancer Diagnosis". Molecules. 22 ( ... Peptide nucleic acid (PNA) FANA (Fluoro Arabino nucleic acid) HNA could be used to potentially act as a drug that can recognize ...
PPIB
PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds and regulate protein folding and maturation. ... "Comparing human pancreatic cell secretomes by in vitro aptamer selection identifies cyclophilin B as a candidate pancreatic ... "Selective assay for CyPA and CyPB in human blood using highly specific anti-peptide antibodies". J. Immunol. Methods. 178 (1): ... this protein catalyzes the cis-trans isomerization of proline imidic peptide bonds, which allows it to regulate protein folding ...
Hapten
Carter, John (1 January 1996). "Conjugation of Peptides to Carrier Proteins via Glutaraldehyde". The Protein Protocols Handbook ... 2009). Affinity probe capillary electrophoresis evaluation of aptamer binding to Campylobacter jejuni bacteria(ARL-TR (Aberdeen ...
Ligase ribozyme
The first such selections involved isolation of various aptamers that bind to small molecules. The first catalytic RNAs ... Robertson MP, Knudsen SM, Ellington AD (January 2004). "In vitro selection of ribozymes dependent on peptides for activity". ...
Small interfering RNA
However recently a large Phase III trial of PEGylated RNA aptamer against factor IX had to be discontinued by Regado ... Shukla RS, Qin B, Cheng K (October 2014). "Peptides used in the delivery of small noncoding RNA". Molecular Pharmaceutics. 11 ( ...
Expanded genetic code
In 2013, they applied the Ds-Px pair to DNA aptamer generation by in vitro selection (SELEX) and demonstrated the genetic ... One study, in fact, found that there were at least 83 peptides majorly affected by the readthrough Additionally, the labelling ... Kimoto M, Yamashige R, Matsunaga K, Yokoyama S, Hirao I (May 2013). "Generation of high-affinity DNA aptamers using an expanded ... There are several techniques to produce peptides chemically, generally it is by solid-phase protection chemistry. This means ...
Cytochrome c
This motif is located towards the N-terminus of the peptide chain and contains a histidine as the 5th ligand of the heme iron. ... Loo JF, Lau PM, Ho HP, Kong SK (October 2013). "An aptamer-based bio-barcode assay with isothermal recombinase polymerase ...
Kinetic exclusion assay
Molecules suited for measurement by KinExA are antibodies, recombinant proteins, small molecules, aptamers, lipids, nanobodies ... "Interactions of HIV-1 inhibitory peptide T20 with the gp41 N-HR coiled coil". The Journal of Biological Chemistry. 284 (6): ...
Fluorescent tag
The method involves fluorescently labeling peptide molecules that would alter an organism's natural pathway. When this peptide ... Likewise, the spinach aptamer is an engineered RNA sequence which can bind GFP chromophore chemical mimics, thereby conferring ... Because of the exact defined change that these isotopes incur on the peptides, it is possible to tell through the spectrometry ... The most commonly labelled molecules are antibodies, proteins, amino acids and peptides which are then used as specific probes ...
RNA
Nissen P, Hansen J, Ban N, Moore PB, Steitz TA (August 2000). "The structural basis of ribosome activity in peptide bond ... structure RNA virus DNA History of RNA Biology List of RNA Biologists RNA Society Macromolecule RNA-based evolution Aptamer RNA ... and the catalysis of peptide bond formation in the ribosome; these are known as ribozymes. According to the length of RNA chain ... determination of the structure of the ribosome-an RNA-protein complex that catalyzes peptide bond formation-revealed that its ...
Force spectroscopy
Ma, Xiao; Gosai, Agnivo; Balasubramanian, Ganesh; Shrotriya, Pranav (May 2019). "Force spectroscopy of the thrombin-aptamer ... RNA Polymerase Targeting Lasso Peptides Produced by Human Isolates of Acinetobacter gyllenbergii and Klebsiella pneumoniae". ... and its DNA aptamer. A recently developed technique, acoustic force spectroscopy (AFS), allows the force manipulation of ...
Antibody mimetic
They are usually artificial peptides or proteins with a molar mass of about 3 to 20 kDa. (Antibodies are ~150 kDa.) Nucleic ... "Rapid identification and development of SARS-CoV-2 selective Optimers". Aptamer Group. Retrieved 2021-08-03. Hantschel O (2017- ...
Immunoproteomics
The technique of peptide mass fingerprinting (PMF) can be used to check a peptide's mass spectrum against a database of protein ... aptamers). Large numbers (dozens to hundreds) of immune-related cytokines and related markers can be simultaneously assayed in ... binding peptides Purification and identification of protein antigens binding specific antibodies (or other affinity reagents) ...
Magnetic resonance imaging
Commonly, peptides, antibodies, or small ligands, and small protein domains, such as HER-2 affibodies, have been applied to ... Liu CH, Ren J, Liu CM, Liu PK (January 2014). "Intracellular gene transcription factor protein-guided MRI by DNA aptamers in ... "Measurement of the degree of coupled isotopic enrichment of different positions in an antibiotic peptide by NMR". Journal of ...
Aptamers as Novel Binding Molecules on an Antimicrobial Peptide-Armored Composite Hydrogel Wound Dressing for Specific Removal...
Aptamers as Novel Binding Molecules on an Antimicrobial Peptide-Armored Composite Hydrogel Wound Dressing for Specific Removal ... Aptamers as Novel Binding Molecules on an Antimicrobial Peptide-Armored Composite Hydrogel ... We demonstrate that this material combining aptamer-mediated affinity and peptide-dependent pathogen eradication can ... A drug-loaded zone of the composite released the C14R antimicrobial peptide to deliver it directly to the bound pathogenic ...
The 2018 Phillip A. Sharp Awards for Innovation in Collaboration | American Association for Cancer Research (AACR)
Aptamer-based Detection and Binding of Peptide-MHC. Cassian Yee, MD, MD Anderson Cancer Center ... Nucleic acid tools, called aptamers, can be used to study specific peptide-MHC complexes on tumor and non-tumor cells. ... The team has begun to select aptamers which can recognize the peptide fragment from the protein called NY-ESO-1. ... on immune cells are responsible for holding on to the peptides in such a way that T cells can recognize the peptides, and kill ...
Targeting intrinsically disordered regions facilitates disco... : PAIN
Small peptides, especially those derived from natural proteins as inhibitory peptide aptamers (iPAs), can produce highly ... AAV-encoded CaV2.2 peptide aptamer CBD3A6K for primary sensory neuron-targeted treatment of established neuropathic pain. Gene ... T-type/Cav3.2 channels\r, Peripheral nervous system\r, Dorsal root ganglia\r, Adeno-associated virus\r, Peptide aptamer\r, ... T-type/Cav3.2 channels; Peripheral nervous system; Dorsal root ganglia; Adeno-associated virus; Peptide aptamer; Neuropathic ...
Biosensors | Free Full-Text | Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in...
No antibodies, aptamers or peptides are required. Once the GMNP-AFB complex is formed, GMNPs facilitate rapid detection due to ... He, F.; Xiong, Y.; Liu, J.; Tong, F.; Yan, D. Construction of Au-IDE/CFP10-ESAT6 aptamer/DNA-AuNPs MSPQC for rapid detection of ... 29], magnetic beads coated with specific peptides yielded an 85-100% CE when used to separate Mycobacterium avium subsp. ... The use of antibodies and peptides for bacterial extraction in a matrix is a common practice. In a study by Foddai et al. [ ...
Staff - The University of Nottingham
Peptide aptamers: Novel Coatings for Orthopaedic Implants Material Science and Engineering C. 54, 83 ... Antimicrobial peptide coatings for hydroxyapatite: Electrostatic and covalent attachment of antimicrobial peptides to surfaces ... Directly bonding antimicrobial peptide mimics to steel and the real world applications of these materials MATERIALS SCIENCE & ... Topical delivery of Anti-VEGF Drugs to the Ocular Posterior Segment using cell penetrating Peptides IOVS. 1(58), 5 ...
Agonistic autoantibodies directed against G-protein-coupled receptors and their relationship to cardiovascular diseases
APS -APS March Meeting 2011
- Event - Overview of single-molecule methods including high-force, force-fluorescence, and dual...
3 Or 4 Amino Acid Residues In The Peptide Chain Patents and Patent Applications (Class 514/21.9) - Justia Patents Search
Search for 3 Or 4 Amino Acid Residues In The Peptide Chain Patents and Patent Applications (Class 514/21.9) Filed with the ... The PAR2 agonists include small organic molecules, antibodies, and aptamers. In addition, the PAR2 agonist may be a PAR2 ... Short peptides having these properties, and methods and uses of such short peptides in clinical and cosmetic applications are ... Dispersion including: a lipid peptide type compound in which peptide portion formed by repetition of at least two or more ...
A synthetic biology approach to fighting Francisella tularensis: Development of aptamer presenting DNA-nanorings
-...
A basis set of de novo coiled-coil peptide oligomers for rational protein design and synthetic biology. Fletcher, J. M., Boyle ... Accessibility, Reactivity, and Selectivity of Side Chains within a Channel of de Novo Peptide Assembly. Burton, A. J., Thomas, ... A synthetic biology approach to fighting Francisella tularensis: Development of aptamer presenting DNA-nanorings. *Brady, R L ( ...
Automotive Camera Market Industry Analysis - Automotive Camera
Aptamers are molecules of peptides or oligonucleotides that bind to a specific protein, lipid, or nucleic acid molecules. ... Aptamers are easily synthesized and used for protein detection, inhibition, and characterization. Aptamers could be… ... The Global Aptamers Market size accounted for USD 1,229 Million in 2021 and is expected to reach the value of USD 5,957 Million ... Aptamers Market Size is Estimated to Reach USD 5,957 Million by 2030 ...
SELECTBIO - Biodetection & Biosensors 2017 Agenda
We here exemplify the use of synthetic molecules such as DNA aptamers and peptide aptamers (Affimers), as alternatives to ... Impedimetric and Field-Effect DNA and Peptide Aptamer Sensors for Medical and Environmental Applications. Pedro Estrela, ... We review recent results developing multiplexed sensors, using aptamer modified nanomaterials and nanopores for the detection ... aptamer-based biorecognition, isotrhermal DNA amplification and text-based readout technologies. Examples will be provided to ...
Recent advances in precision oncology research | npj Precision Oncology
The nanobots were programmed to attach only to cancer cells by including a DNA aptamer (i.e., oligonucleotide or peptide ... The motor was created in several sizes and could carry peptides designed to target and kill specific cancer cells. The ... In this case the DNA aptamer directly targeted nucleolin, which is specifically found on tumor-associated endothelial cells. ...
Label-free characterization of biomolecular interactions
Meyer and Nelson on a Failed Explanation for the Origin of the Genetic Code | Evolution News
... no evidence shows that amino acids thus carried by the RNA aptamers would form peptide bonds, especially in any realistic ... The DRT model RNA aptamers, however, bind amino acids using weaker non-covalent associations. As a result, the RNA aptamers ... Ordered peptides themselves are envisioned as being of value in the RNA-based biosphere such that there was a selection for the ... even if an ensemble of RNA aptamers aligned in close proximity to one another, and even if they did so in a way that would in ...
Bifidobacterium bifidum agent in tumour treatment | IJN
... peptides or aptamers have to conjugate with NPs to prepare targeting nanoparticles. Aptamers are single-stranded DNA or RNA ... The aptamer CCFM641-5 (AP) is an aptamer specifically for BF and was used to guide targeting binding between BF and AP-PFH/PLGA ... 5ʹ-NH2-modified CCFM641-5 aptamer and 5ʹ-FAM-modified CCFM641-5 aptamer were synthesized by Sangon Biotech Co., Ltd (Shanghai, ... Guided by aptamers, AP-PFH/PLGA NPs can target BF in tumor. In the third step, when a large amount of NPs accumulate in the ...
NIOSHTIC-2 Search Results - Full View
Recombinant (E.Coli) Herpes Simplex Virus-2 gG (HSV-2 gG)
Primary Antibodies
Alpha Diagnostic Intl. Inc., enabling innovative research from Adenovirus Virus to Zika Virus
Vaccigel ELISA, Infectious Disease, Vaccine ELISA, Peptides, Animal Diseases & Vaccine ELISA, Animal Health Tests-Laboratory ... Aptamers, Adjuvants, ODNs, IHC/ICC Slides, Lysates, TruStrip Rapid Tests, ELISA instruments, Vaccigel ELISA, Humanized ... home of Vaccine ELISA, custom antibodies & peptides, ELISA kits from Adenovirus Virus to Zika Virus. Humanized Therapeutic ...
Publications - Laboratoire de Biologie et Modélisation de la Cellule
... a case study of site-directed low-frequency random mutagenesis for dissecting target specificity of peptide aptamers.. Mol Cell ... a case study of site-directed low-frequency random mutagenesis for dissecting target specificity of peptide aptamers.. Mol Cell ... Application to peptide conformers.. J Phys Chem B, 113(29):9728-40. ...
Protein arrays : methods and protocols | WorldCat.org
Knowledge Centre - Innovate UK KTN
Heat shock proteins are crucial players in the introduction of cancer - Genomics Proteomics and Bioinformatics
... two business lead peptide aptamers are under analysis: PA11 and PA50. Like the little molecule inhibitors of hsp27, peptide ... The second method of concentrating on hsp27 utilizes peptide aptamers that bind towards the proteins and disrupt buy 537705-08- ... peptide aptamers both bind right to hsp27 protein and disrupt its function. C) Antisense oligonucleotide OGX-427 binds towards ... Proteins aptamers are little amino acidity sequences that can bind to a particular proteins area.40 The aptamer was created to ...
What are Anti-PEG Antibodies and Why are they Useful?
Danicopan, an Oral Complement Factor D Inhibitor, Exhibits High and Sustained Exposure in Ocular Tissues in Preclinical Studies...
International Symposium on Molecular Imprinting Technology for Biomedical Applications (Biomed MIT 2022)
Aptamer Based Imprinting of SS DNA for Biodetection. 22:30 CST / 16:30 CEST. Keynote. Yan Zhao Molecularly imprinted ... Biomimetic Peptide Binders and Affinity Nanointerfaces for Synergetic Cancer Theranostics. 17:55 CST / 11:55 CEST. Invited. ... Molecular imprinting with DNA aptamers and nanozymes. 21:00 CST / 15:00 CEST. Invited. Huiqi Zhang. Complex biological sample- ... Preparation of two different peptide-MIPs for cancer remedy. 17:15 CST / 11:15 CEST. Invited. Kaiguang Yang. Molecular ...
Aptamer Group signs deal with Flip Gene Therapeutics to support the development of inducible gene therapies
... proteins and peptides and cell targets. Delivering the potential for increased success in discovery over a broader target range ... Aptamer Group signs deal with Flip Gene Therapeutics to support the development of inducible gene therapies. 16-Jun-2022 ... Aptamer Group, the developer of novel Optimer binders to enable innovation in the life sciences industry, has negotiated a deal ... Dr Arron Tolley, CEO of Aptamer Group, said: "I am delighted to have established this agreement to develop Optimer binders to ...
CEbiotech - be visible on tle BioBusiness market
Are aptamers set to become mainstream? | Avacta Life Sciences Limited
... these difficulties because the Affimer peptide structure presents none of the chemical limitations associated with aptamers, ... This technology was showcased by selecting aptamers to ?-thrombin and human factors IXa, X and D, with the identified aptamers ... These include limitations in the aptamer library being screened, the retention of non-specific aptamers during the screening ... where the enriched pool of aptamers are amplified to be used in the next round of selection. Finally, the enriched aptamer pool ...
ProteinsProteinAntibodiesAffinityPolyclonalAntimicrobial peptideMolecules that bindSELEXDaltonsSpecificityTargetsAntibodySyntheticBindersTertiaryNucleicBiomarkersSequencesBioinformaticsLigandsPathogenicIterativeFormulationsCandidatesVivoProbeProcessesAmino acidsAmplificationTissuesTargetRoundsSmallMechanismFunctionalAnalysisTechnologyEffectiveDevelopDrug delivery
Proteins11
- Small peptides, especially those derived from natural proteins as inhibitory peptide aptamers (iPAs), can produce highly effective and selective blockade of specific nociceptive molecular pathways to reduce pain with minimal off-target effects. (lww.com)
- In this context, immunoadsorption using peptides/proteins or aptamers as binders are introduced. (nih.gov)
- Ubiquitous in all life forms and central to any research or development program involving biomolecules, the interactions between proteins, nucleic acids, peptides and other biomacromolecules lie at the heart of biology, physiology, and medicine. (wyatt.com)
- The 1st involves developing little substances that bind towards the proteins straight and inhibit its function.23, 24 The next utilizes proteins aptamers that bind the proteins and disrupt function.25 The 3rd approach employs an antisense oligonucleotide (ASO), which targets the mRNA that encodes for hsp27, thus avoiding translation from the protein. (bioinf.org)
- The second method of concentrating on hsp27 utilizes peptide aptamers that bind towards the proteins and disrupt buy 537705-08-1 its function (Body 3b). (bioinf.org)
- Proteins aptamers are little amino acidity sequences that can bind to a particular proteins area.40 The aptamer was created to outcompete the protein that could bind compared to that domain, thus inhibiting its function. (bioinf.org)
- Due to these unique characteristics, PEG has found itself being used in the field of biopharmaceuticals as a coating for drugs made of proteins, peptides, drug-antibody conjugates, aptamers, or monoclonal antibodies to facilitate their delivery. (news-medical.net)
- The Optimer platform consists of three parallel target-type specific discovery processes for small molecules, proteins and peptides and cell targets. (manufacturingchemist.com)
- Custom develop aptamers against small to large proteins. (aptagen.com)
- Project 4 will develop aptamers to target proteins and will work with Project 5 to develop antibodies to target proteins that will go to Projects 1 and 2 for ex vivo nanosenor development. (stanford.edu)
- Caught in action: selecting Peptide aptamers against intrinsically disordered proteins in live cells. (vu.nl)
Protein6
- Ordered peptides themselves are envisioned as being of value in the RNA-based biosphere such that there was a selection for the origination, and subsequent incremental enhancement, of protein-synthesizing (translation) machinery. (evolutionnews.org)
- Coupling newly developed nanomaterials with other recognition elements such as nanobodies, peptide aptamers, and protein receptors would open up new avenues for electrochemical immunoassays and further extend their applications. (cdc.gov)
- The authors present protocols to create and immobilize the capture substrate-the first task in designing a protein array-using a variety of affinity capture reagents, including antibodies, peptides, aptamers, biotin, chemical reagents, and chromatographic substrates. (worldcat.org)
- Designing of peptide aptamer targeting the receptor-binding domain of spike protein of SARS-CoV-2: an in silico study. (cdc.gov)
- First, we will use mass spectrometry to characterize tumors/serum for protein profiles, and then we will develop corresponding antibodies/aptamers that will be linked to nanosensors for high sensitivity target cell surface antigens and potentially intracellular targets. (stanford.edu)
- These tools contribute to analysis of various aspects in the designing of RNA aptamers like tertiary structure prediction of RNA, protein folding, motif prediction, database search and structure analysis and design. (biotecharticles.com)
Antibodies1
- While antibodies are highly effective for a wide range of applications, there are unique aptamer advantages that can overcome some difficult scientific challenges. (mamul.am)
Affinity7
- We demonstrate that this material combining aptamer-mediated affinity and peptide -dependent pathogen eradication can quantitatively remove bacterial cells from the " wound " surface, and we show that the surface-trapped bacteria are completely killed. (bvsalud.org)
- These advantages over the standard antibody affinity reagent have meant that aptamers are well placed to serve as antibody alternatives to the research, diagnostic and therapeutic markets. (avacta.com)
- Despite the multiple rounds of panning involved in SELEX aimed to increase the stringency in aptamer selection, all too often this process fails to deliver an aptamer with the required characteristics to be used as an affinity reagent. (avacta.com)
- These include limitations in the aptamer library being screened, the retention of non-specific aptamers during the screening rounds, the accumulation of amplification artefacts that reduce yields and impede the enrichment of a high-affinity aptamer pool and the unintended isolation of candidate aptamers lacking sufficient specificity for the target due, in part, to the use of ion-exchange binding mechanisms that do not reflect therapeutic activity. (avacta.com)
- Affimer technology overcomes these difficulties because the Affimer peptide structure presents none of the chemical limitations associated with aptamers, allowing high-affinity, specific Affimer binders to truly be developed to any target. (avacta.com)
- While the need for antibody alternatives is increasing the problems inherent in aptamers makes Affimer technology the optimal affinity reagent for researchers. (avacta.com)
- Epitope peptides of aptamer‐ C‐Met complexes were identified by proteolytic affinity‐ mass spectrometry in combination with SPR biosensor analysis (PROTEX‐SPR‐MS), using high‐pressure proteolysis for efficient digestion. (pressurebiosciences.com)
Polyclonal3
- A two-layered hydrogel composite material was constructed based on an established eight-membered focused anti-P. aeruginosa polyclonal aptamer library , which was chemically crosslinked to the material surface to form a trapping zone for efficient binding of the pathogen. (bvsalud.org)
- The enriched polyclonal pool is sequenced and thousands of aptamer sequences are provided. (aptagen.com)
- Client will have access to all the data generated from the polyclonal aptamer pool in Phase I (millions of sequences). (aptagen.com)
Antimicrobial peptide2
- A drug -loaded zone of the composite released the C14R antimicrobial peptide to deliver it directly to the bound pathogenic cells . (bvsalud.org)
- Directly bonding antimicrobial peptide mimics to steel and the real world applications of these materials MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS. (nottingham.ac.uk)
Molecules that bind1
- Aptamers are peptide molecules that bind to a given specific target molecule. (biotecharticles.com)
SELEX5
- Aptamers are generated by SELEX, which is an iterative in vitro process entailing three main steps: 1) binding, where the target molecule is incubated with a random library of oligonucleotide aptamers 2) separation of the target bound aptamers from unbound ones and 3) amplification, where the enriched pool of aptamers are amplified to be used in the next round of selection. (avacta.com)
- es in mind the researchers developed a new platform, termed high-fidelity SELEX or Hi-Fi SELEX, that they hope will accelerate and improve the selection of DNA aptamers by overcoming some of the limitations of the current SELEX methodology. (avacta.com)
- Here we report a molecular interaction study of human C‐Met expressed in kidney cells with two DNA aptamers of 60 and 64 bases, obtained using the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure. (pressurebiosciences.com)
- Aptamers are developed through the SELEX process and over the years these have become primary tools for aptamer synthesis. (mamul.am)
- Over the past three decades, since the invention of SELEX by Larry Gold and Jack William in the 1990, the technology has seen several modifications to streamline and standardized aptamer-isolation process. (mamul.am)
Daltons1
- Flexibility of aptamers offer wider applications as they can be developed against molecules as small as 60 Daltons this means aptamers can target molecules that are ten times smaller than antibody targets. (mamul.am)
Specificity1
- Calcineurin A versus NS5A-TP2/HD domain containing 2: a case study of site-directed low-frequency random mutagenesis for dissecting target specificity of peptide aptamers. (ens-lyon.fr)
Targets3
- The agreement will see Aptamer Group develop Optimer binders to several small molecule targets. (manufacturingchemist.com)
- Together these limitations may ultimately prevent the development of aptamers to therapeutically relevant targets. (avacta.com)
- At least 200 aptamer candidates are synthesized on microarray for semi-quantitative assessment against positive and counter targets to determine the top 5 candidates for further characterization. (aptagen.com)
Antibody1
- This indicates a high unmet need of patients thus, paving a way for aptamer - based solutions due to their advantages over traditional antibody-based therapies. (mamul.am)
Synthetic2
- Specificities and affinities were ascertained by SPR analysis of the synthetic epitope peptides. (pressurebiosciences.com)
- A synthetic form of exendin-4, a 39-amino acid peptide isolated from the venom of the Gila monster lizard (Heloderma suspectum). (nih.gov)
Binders2
- Aptamer Group, the developer of novel Optimer binders to enable innovation in the life sciences industry, has negotiated a deal with the biotechnology company Flip Gene Therapeutics to support its novel, inducible gene therapy platform with the use of Optimer technology. (manufacturingchemist.com)
- Dr Arron Tolley, CEO of Aptamer Group, said: "I am delighted to have established this agreement to develop Optimer binders to enable inducible treatments in this exciting field of gene therapy. (manufacturingchemist.com)
Tertiary1
- Additionally, aptamers do not require immune response and binding of tertiary aptamer structures to target molecules. (mamul.am)
Nucleic2
- Other noteworthy developments in modifications of aptamers, include the addition of functional units of non-nucleic origin and nucleotide modifications, which further improves aptamer performance. (mamul.am)
- Target molecule is incubated along with the oligonucleotide library allowing for nucleic acids to bind to them resulting in the formation of aptamers. (mamul.am)
Biomarkers1
- C‐Met aptamers have been considered a useful tool for detection of cancer biomarkers. (pressurebiosciences.com)
Sequences1
- A linear epitope, C‐Met (381‐393) was identified for CLN0004, while the CLN0003 aptamer revealed an assembled epitope comprised of two peptide sequences, C‐Met (524‐543) and C‐Met (557‐568). (pressurebiosciences.com)
Bioinformatics3
- Finally, the enriched aptamer pool is analysed by high-throughput sequencing and bioinformatics analysis to identify candidate aptamers. (avacta.com)
- The most promising aptamer candidates are identified using proprietary bioinformatics. (aptagen.com)
- Understanding their basic mechanism, processes and creation of RNA aptamers is where the concept of in silico designing comes into the picture In silico designing of RNA aptamers has gained much achievement due to the use of easy to use and apply bioinformatics tool. (biotecharticles.com)
Ligands1
- Single stranded oligonucleotide ligands, known as aptamers, can be raised to theoretically any target and have rapid development times of up to 12 weeks. (avacta.com)
Pathogenic2
- Here we present for the first time a potential wound dressing material implementing aptamers as binding entities to remove pathogenic cells from newly contaminated surfaces of wound matrix-mimicking collagen gels . (bvsalud.org)
- Aptamer development process does not involve animals or living cells thus, making it possible to select aptamers for toxic compounds such as zootoxins and pathogenic bacteria. (mamul.am)
Iterative1
- A method of generating a large library of randomized nucleotides and selecting NUCLEOTIDE APTAMERS by iterative rounds of in vitro selection. (bvsalud.org)
Formulations1
- Stability of Cell-Penetrating Peptide anti-VEGF Formulations for the Treatment of Age-Related Macular Degeneration Global Challenges. (nottingham.ac.uk)
Candidates1
- To ensure the development of more effective aptamer-based therapies candidates, several players have developed novel aptamer technologies that can be used for research, drug delivery and diagnostic applications. (mamul.am)
Vivo2
- The use of peptides or aptamers for in vivo neutralization of GPCR-AABs is also described. (nih.gov)
- Custom develop aptamers targeting organs and tissues in vivo for drug delivery or imaging applications. (aptagen.com)
Probe1
- Force spectroscopy is employed to probe the strength of single peptide aptamer bonds. (aps.org)
Processes1
- Understanding their basic mechanism, processes and creation of RNA aptamers is where the concept of in silico designing comes into the picture In silico designing of RNA aptamers has gained mu. (biotecharticles.com)
Amino acids2
- Yarus and his supporters maintain that specific peptides were first made by ordering carboxyl-activated amino acids using amino acid sites in an RNA template. (evolutionnews.org)
- A modified procedure substitutes AMINO ACIDS in place of NUCLEOTIDES to make PEPTIDE APTAMERS . (bvsalud.org)
Amplification1
- The talk will focus on the development of printable components to produce easily manufactured bioactive paper sensors with a range of capabilities, including integrated sample preparation, aptamer-based biorecognition, isotrhermal DNA amplification and text-based readout technologies. (selectbiosciences.com)
Tissues1
- Custom develop aptamers against various tissues. (aptagen.com)
Target2
- The chemistry of the target-display surface and composition of the equilibration solvent were also engineered to inhibit the non-specific retention of aptamers improving partition efficiencies. (avacta.com)
- Custom develop aptamers against your unique target. (aptagen.com)
Rounds2
- This platform is designed to enhance the diversity of the initial ap tamer library and reduce the number of selection rounds required to identify therapeutically relevant aptamers. (avacta.com)
- thrombin and human factors IXa, X and D, with the identified aptamers offering nM order dissociation constants (Kd) achieved within three selection rounds. (avacta.com)
Small1
- Currently, aptamers for small molecule drugs, peptides, dyes and viral particles have been developed successfully. (mamul.am)
Mechanism1
- Ma Wentao describes the DRT scenario as "attractive because it should be the simplest mechanism for RNA to synthesize peptides, thus very likely to have been adopted initially in the RNA world. (evolutionnews.org)
Functional1
- To achieve this blocking elements in the form of complimentary oligonucleotides were bound to the constant regions of the aptamer to prevent the aptamer folding upon itself, thus preserving the functional diversity of the library. (avacta.com)
Analysis2
- Presently, two business lead peptide aptamers are under analysis: PA11 and PA50. (bioinf.org)
- High affinities (K D , 80‐510 nM) were determined for aptamer‐ C‐Met complexes, with two‐step binding suggested by kinetic analysis. (pressurebiosciences.com)
Technology1
- The present technology provides compositions related to aromatic-cationic peptides linked to a therapeutic biological molecule and uses of the same. (justia.com)
Effective1
- Like the little molecule inhibitors of hsp27, peptide aptamers aren't effective independently but are accustomed to sensitize malignancies to various other therapies. (bioinf.org)
Develop2
- Custom develop aptamers against peptides. (aptagen.com)
- Custom develop aptamers against transfected cell lines for surface binding or internal targeting. (aptagen.com)
Drug delivery1
- We are inventing the future of medicine by developing powerful new peptides for drug delivery into the central nervous system, hydorgels that "sense" and "respond" to the tissue micro-environment, and stem cell reprogramming strategies to drive neurogenesis in the injured spinal cord. (uw.edu)