Aprotinin
A single-chain polypeptide derived from bovine tissues consisting of 58 amino-acid residues. It is an inhibitor of proteolytic enzymes including CHYMOTRYPSIN; KALLIKREIN; PLASMIN; and TRYPSIN. It is used in the treatment of HEMORRHAGE associated with raised plasma concentrations of plasmin. It is also used to reduce blood loss and transfusion requirements in patients at high risk of major blood loss during and following open heart surgery with EXTRACORPOREAL CIRCULATION. (Reynolds JEF(Ed): Martindale: The Extra Pharmacopoeia (electronic version). Micromedex, Inc, Englewood, CO, 1995)
Hemostatics
Agents acting to arrest the flow of blood. Absorbable hemostatics arrest bleeding either by the formation of an artificial clot or by providing a mechanical matrix that facilitates clotting when applied directly to the bleeding surface. These agents function more at the capillary level and are not effective at stemming arterial or venous bleeding under any significant intravascular pressure.
Antifibrinolytic Agents
Aminocaproic Acid
Diatomaceous Earth
A form of SILICON DIOXIDE composed of skeletons of prehistoric aquatic plants which is used for its ABSORPTION quality, taking up 1.5-4 times its weight in water. The microscopic sharp edges are useful for insect control but can also be an inhalation hazard. It has been used in baked goods and animal feed. Kieselguhr is German for flint + earthy sediment.
Postoperative Hemorrhage
Cardiopulmonary Bypass
Aminocaproates
Blood Transfusion
Kallikreins
Proteolytic enzymes from the serine endopeptidase family found in normal blood and urine. Specifically, Kallikreins are potent vasodilators and hypotensives and increase vascular permeability and affect smooth muscle. They act as infertility agents in men. Three forms are recognized, PLASMA KALLIKREIN (EC 3.4.21.34), TISSUE KALLIKREIN (EC 3.4.21.35), and PROSTATE-SPECIFIC ANTIGEN (EC 3.4.21.77).
Kaolin
The most common mineral of a group of hydrated aluminum silicates, approximately H2Al2Si2O8-H2O. It is prepared for pharmaceutical and medicinal purposes by levigating with water to remove sand, etc. (From Merck Index, 11th ed) The name is derived from Kao-ling (Chinese: "high ridge"), the original site. (From Grant & Hackh's Chemical Dictionary, 5th ed)
Protease Inhibitors
Intraoperative Care
Kinins
A generic term used to describe a group of polypeptides with related chemical structures and pharmacological properties that are widely distributed in nature. These peptides are AUTACOIDS that act locally to produce pain, vasodilatation, increased vascular permeability, and the synthesis of prostaglandins. Thus, they comprise a subset of the large number of mediators that contribute to the inflammatory response. (From Goodman and Gilman's The Pharmacologic Basis of Therapeutics, 8th ed, p588)
Thoracic Surgical Procedures
Fibrinolysin
A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.
Circulatory Arrest, Deep Hypothermia Induced
Encyclopedias as Topic
Trypsin Inhibitors
Hypotension
Trypsin Inhibitor, Kazal Pancreatic
Insulin-like growth factors I and II are unable to form and maintain their native disulfides under in vivo redox conditions. (1/706)
Insulin-like growth factor (IGF) I does not quantitatively form its three native disulfide bonds in the presence of 10 mM reduced and 1 mM oxidized glutathione in vitro [Hober, S. et al. (1992) Biochemistry 31, 1749-1756]. In this paper, we show (i) that both IGF-I and IGF-II are unable to form and maintain their native disulfide bonds at redox conditions that are similar to the situation in the secretory vesicles in vivo and (ii) that the presence of protein disulfide isomerase does not overcome this problem. The results indicate that the previously described thermodynamic disulfide exchange folding problem of IGF-I in vitro is also present in vivo. Speculatively, we suggest that the thermodynamic disulfide exchange properties of IGF-I and II are biologically significant for inactivation of the unbound growth factors by disulfide exchange reactions to generate variants destined for rapid clearance. (+info)Activation of Xenopus eggs by proteases: possible involvement of a sperm protease in fertilization. (2/706)
Egg activation in cross-fertilization between Xenopus eggs and Cynops sperm may be caused by a protease activity against Boc-Gly-Arg-Arg-MCA in the sperm acrosome. To determine the role of the sperm protease in fertilization, the protease was purified from Cynops sperm using several chromatographic techniques. We found that purified sperm protease readily hydrolyzes Boc-Gly-Arg-Arg-MCA and Z-Arg-Arg-MCA, that protease activity was inhibited by the trypsin inhibitors aprotinin and leupeptin, and that not only the purified protease, but also cathepsin B, induces activation in Xenopus eggs. We inseminated unfertilized Xenopus eggs with homologous sperm in the presence of various peptidyl MCA substrates or protease inhibitors and demonstrated that trypsin inhibitors or MCA substrates containing Arg-Arg-MCA reversibly inhibited fertilization of both fully jellied and denuded eggs. Sperm motility was not affected by the reagents. An extract obtained from Xenopus sperm showed hydrolytic activity against Boc-Gly-Arg-Arg-MCA, Z-Arg-Arg-MCA, and Arg-MCA. These results suggest that the tryptic protease in Xenopus sperm is involved in fertilization, most likely by participating in egg activation. (+info)Allosteric modulation of BPTI interaction with human alpha- and zeta-thrombin. (3/706)
In this study, thrombin interaction with the basic pancreatic trypsin inhibitor (BPTI) was investigated in the presence of different allosteric modulators of thrombin, that is the C-terminal hirudin peptide 54-65 (Hir54-65), a recombinant thrombomodulin form (TMEGF4-6) and Na+. BPTI binding to alpha-thrombin is positively linked to Na+. Under low sodium concentration (5 mM Na+) the BPTI affinity for alpha-thrombin was roughly threefold lower than in the presence of 150 mM sodium (Ki = 320 microM vs. 100 microM). The hirudin fragment, which binds to the fibrinogen recognition site (FRS) of thrombin, induced a progressive and saturable decrease (3.6-fold) of alpha-thrombin affinity for BPTI, whereas the thrombomodulin peptide, which binds to a more extended region of FRS, caused a 5.5-fold increase of the enzyme affinity for the inhibitor. The opposite effect exerted by Hir54-65 and TMEGF4-6 was also observed for BPTI interaction with zeta-thrombin, in which the amidic bond between W148 and T149 is cleaved. However, in this case the effect by Hir54-65 and TMEGF4-6, although qualitatively similar to that observed with alpha-thrombin, had a smaller magnitude. Thrombin hydrolysis of Protein C was also differently affected by Hir54-65 and TMEGF4-6 peptides. While the latter enhanced the Protein C activation, the former caused a reduction of both alpha- and zeta-thrombin kcat/K(m)' for Protein C cleavage. These results showed that (a) Na+ facilitates BPTI interaction with thrombin; (b) Hir54-65 and TMEGF4-6, though sharing in part the same binding site at the thrombin FRS, can affect in opposite way thrombin's interaction with BPTI and Protein C; (c) such findings along with the results obtained with zeta-thrombin might be explained by admitting that the thermodynamic linkage between FRS and the critical W60-loop is also controlled by ligation and/or conformational state of the W148 insertion loop. (+info)Strategy for balancing anticoagulation and hemostasis in aortocoronary bypass surgery: blood conservation and graft patency. (4/706)
The minimal effective dose of aprotinin on hemostasis under normothermic perfusion, the influence of anticoagulant therapy on graft patency, and the thromboembolic and hemorrhagic events were investigated after aortocoronary bypass graft operation (CABG). One hundred CABG patients under normothermic perfusion were randomly divided into the following groups: (1) coumadin plus acetylsalicylic acid (ASA) (n=32); no aprotinin used during cardiopulmonary bypass (CPB); (2) minimal-dose, 10(6) KIU during CPB, aprotinin used, followed by ASA and coumadin (n=36); and (3) very low-dose, total of 2x10(6) KIU before CPB and during CPB; aprotinin used; anticoagulation therapy with heparin early after surgery and followed by replacement with ASA and coumadin (n=32). The patency of arterial grafts was 100% in all groups. The patency of vein grafts was 95-98% and there was no difference among the groups. The blood loss was significantly reduced in both aprotinin groups (groups 2 and 3) compared to the coumadin plus ASA group, although no difference existed between the 2 aprotinin groups. Postoperative thrombotic and hemorrhagic events were not observed in any group. From this study, it was concluded that 10(6) KIU aprotinin in pump-prime-only followed by oral ASA and coumadin was the recommendation from the benefit/cost consideration. (+info)Comparison of anionic and cationic trypsinogens: the anionic activation domain is more flexible in solution and differs in its mode of BPTI binding in the crystal structure. (5/706)
Unlike bovine cationic trypsin, rat anionic trypsin retains activity at high pH. This alkaline stability has been attributed to stabilization of the salt bridge between the N-terminal Ile16 and Asp194 by the surface negative charge (Soman K, Yang A-S, Honig B, Fletterick R., 1989, Biochemistry 28:9918-9926). The formation of this salt bridge controls the conformation of the activation domain in trypsin. In this work we probe the structure of rat trypsinogen to determine the effects of the surface negative charge on the activation domain in the absence of the Ile16-Asp194 salt bridge. We determined the crystal structures of the rat trypsin-BPTI complex and the rat trypsinogen-BPTI complex at 1.8 and 2.2 A, respectively. The BPTI complex of rat trypsinogen resembles that of rat trypsin. Surprisingly, the side chain of Ile16 is found in a similar position in both the rat trypsin and trypsinogen complexes, although it is not the N-terminal residue and cannot form the salt bridge in trypsinogen. The resulting position of the activation peptide alters the conformation of the adjacent autolysis loop (residues 142-153). While bovine trypsinogen and trypsin have similar CD spectra, the CD spectrum of rat trypsinogen has only 60% of the intensity of rat trypsin. This lower intensity most likely results from increased flexibility around two conserved tryptophans, which are adjacent to the activation domain. The NMR spectrum of rat trypsinogen contains high field methyl signals as observed in bovine trypsinogen. It is concluded that the activation domain of rat trypsinogen is more flexible than that of bovine trypsinogen, but does not extend further into the protein core. (+info)Effect of glycerol on the interactions and solubility of bovine pancreatic trypsin inhibitor. (6/706)
The effects of additives used to stabilize protein structure during crystallization on protein solution phase behavior are poorly understood. Here we investigate the effect of glycerol and ionic strength on the solubility and strength of interactions of the bovine pancreatic trypsin inhibitor. These two variables are found to have opposite effects on the intermolecular forces; attractions increase with [NaCl], whereas repulsions increase with glycerol concentration. These changes are mirrored in bovine pancreatic trypsin inhibitor solubility where the typical salting out behavior for NaCl is observed with higher solubility found in buffers containing glycerol. The increased repulsions induced by glycerol can be explained by a number of possible mechanisms, all of which require small changes in the protein or the solvent in its immediate vicinity. Bovine pancreatic trypsin inhibitor follows the same general phase behavior as other globular macromolecules where a robust correlation between protein solution second virial coefficient and solubility has been developed. This study extends previous reports of this correlation to solution conditions involving nonelectrolyte additives. (+info)Hydroiodic acid attachment kinetics as a chemical probe of gaseous protein ion structure: bovine pancreatic trypsin inhibitor. (7/706)
The kinetics of attachment of hydroiodic acid (HI) to the (M + 6H)6+ ions of native and reduced forms of bovine pancreatic trypsin inhibitor (BPTI) in the quadrupole ion trap environment are reported. Distinctly nonlinear (pseudo first-order) reaction kinetics are observed for reaction of the native ions, indicating two or more noninterconverting structures in the parent ion population. The reduced form, on the other hand, shows very nearly linear reaction kinetics. Both forms of the parent ion attach a maximum of five molecules of hydroiodic acid. This number is expected based on the amino acid composition of the protein. There is a total of 11 strongly basic sites in the protein (i.e., six arginines, four lysines, and one N-terminus). An ion with protons occupying six of the basic sites has five available for hydroiodic acid attachment. The kinetics of successive attachment of HI to the native and reduced forms of BPTI also differ, particularly for the addition of the fourth and fifth HI molecules. A very simple kinetic model describes the behavior of the reduced form reasonably well, suggesting that all of the neutral basic sites in the reduced BPTI ions have roughly equal reactivity. However, the behavior of the native ion is not well-described by this simple model. The results are discussed within the context of differences in the three-dimensional structures of the ions that result from the presence or absence of the three disulfide linkages found in native BPTI. The HI reaction kinetics appears to have potential as a chemical probe of protein ion three-dimensional structure in the gas phase. Hydroiodic acid attachment chemistry is significantly different from other chemistries used to probe three-dimensional structure and hence, promises to yield complementary information. (+info)Urokinase receptor (uPAR, CD87) is a platelet receptor important for kinetics and TNF-induced endothelial adhesion in mice. (8/706)
BACKGROUND: Urokinase plasminogen activator receptor (uPAR, CD87) is a widely distributed 55-kD, glycoprotein I-anchored surface receptor. On binding of its ligand uPA, it is known to increase leukocyte adhesion and traffic. Using genetically deficient mice, we explored the role of uPAR in platelet kinetics and TNF-induced platelet consumption. METHODS AND RESULTS: Anti-uPAR antibody stained platelets from normal (+/+) but not from uPAR-/- mice, as seen by fluorescence-activated cell sorter analysis. 51Cr-labeled platelets from uPAR-/- donors survived longer than those from +/+ donors when injected into a +/+ recipient. Intratracheal TNF injection induced thrombocytopenia and a platelet pulmonary localization, pronounced in +/+ but absent in uPAR-/- mice. Aprotinin, a plasmin inhibitor, decreased TNF-induced thrombocytopenia. TNF injection markedly reduced the survival and increased the pulmonary localization of 51Cr-labeled platelets from +/+ but not from uPAR-/- donors, indicating that it is the platelet uPAR that is critical for their response to TNF. As seen by electron microscopy, TNF injection increased the number of platelets and polymorphonuclear neutrophils (PMNs) in the alveolar capillaries of +/+ mice, whereas in uPAR-/- mice, platelet trapping was insignificant and PMN trapping was slightly reduced. Platelets within alveolar capillaries of TNF-injected mice were activated, as judged from their shape, and this was evident in +/+ but not in uPAR-/- mice. CONCLUSIONS: These results demonstrate for the first time the critical role of platelet uPAR for kinetics as well as for activation and endothelium adhesion associated with inflammation. (+info)
Aprotinin Injection (marketed as Trasylol) Information | FDA
Cytokine Release and Neutrophil Activation Are Not Prevented by Heparin-Coated Circuits and Aprotinin Administration -...
Trasylol | McCarthy & Kelly LLP
On the interaction of bovine pancreatic trypsin inhibitor with maxi Ca(2+)-activated K+ channels. A model system for analysis...
Aprotinin - Wikipedia
Effects of Aprotinin on Gene Expression and Protein Synthesis After Ischemia and Reperfusion in Rats | Circulation
Trasylol (aprotinin) - licence suspended in the UK November 2007
Aprotinin - DrugBank
Lyophilized Powder, Recombinant Aprotinin, Recombinant Trypsin Inhibitor
Pancreatic trypsin inhibitor Mouse anti-Bovine, Alexa Fluor 750, Clone:
| Fisher Scientific
Trasylol
Full Publication List | Wagner Lab
Inhibition of tryptase TL2 from human T4(+) lymphocytes and inhibition of HIV-1 replication in H9 cells by recombinant...
Trasylol (Aprotinin injection) - FindLaw
Letter by Augoustides Regarding Article Aprotinin Does Not Increase the Risk of Renal Failure in Cardiac Surgery Patients |...
Aprotinin for coronary bypass operations: Efficacy, safety, and influence on early saphenous vein graft patency: A multicenter,...
Evolution, expansion and expression of the Kunitz/BPTI gene family associated with long-term blood feeding in Ixodes Scapularis...
Aprotinin Use and Renal Outcome in Hypothermic Bypass and Circulatory Arrest for Surgical Repair of Thoracic Aorta. - Full Text...
RCSB PDB - 1BTH: STRUCTURE OF THROMBIN COMPLEXED WITH BOVINE PANCREATIC TRYPSIN INHIBITOR
Structure Cluster
- 1BTI: CREVICE-FORMING MUTANTS IN THE RIGID CORE OF BOVINE PANCREATIC TRYPSIN INHIBITOR: CRYSTAL...
Protein Disulfide-Isomerase Interacts with a Substrate Protein at All Stages along Its Folding Pathway
10:12 am
Estudo comparativo do emprego da aprotinina em baixas doses X placebo, durante a circulação extracorpórea
The intra-operative use of trasylol (aprotinin) in liver transplantation | SpringerLink
Trasylol caused thousands of deaths that could have been avoided | Health Imaging
Chance News 13 - ChanceWiki
Search Results | Anesthesiology | American Society of Anesthesiologists
Study finds kidney risks in Bayers Trasylol - Medical Marketing and Media
Lawsuits Filed in the Wake of Bayer s Withdrawal of Trasylol From the Market | WebWire
Trasylol Litigation: April 2008 Update
1taw - Proteopedia, life in 3D
Aprotinin and hemostasis in cardiopulmonary bypass<...
Leukocyte filtration and aprotinin: Synergistic anti-inflammatory protection<...
Bayer’s Heart-Surgery Drug Increases Risk of Death - NewsInfernoNewsInferno
Global Aprotinin Sales Market Report 2016 : ReportsnReports
DMOZ - Health: Pharmacy: Drugs and Medications: A: Aprotinin
Fusion proteins comprising annexin V and Kunitz protease inhibitors are highly potent thrombogenic site-directed anticoagulants...
SCOP 2.04 - JenaLib Tree Browser
3btd - Proteopedia, life in 3D
What is happening in and out of a system, simulation tells all.. | rasalsi
Temperature dependence of protein... preview & related info | Mendeley
Guide of causes of renal failur and strokes following coronary surgery
Cardiac Operation : Aprotinin Vs. Heparin Sodium Drugs - DrugInformer
Aprotinin
Aprotinin Side Effects: Common, Severe, Long Term - Drugs.com
The effect of aprotinin on outcome after coronary-artery bypass grafting.
Sequence and Conformational Specificity in Substrate Recognition Several Human Kunitz Protease Inhibitor Domains are Specific...
Aprotinin for coronary artery bypass grafting: Effect on postoperative renal function<...
Evelyn Pringles Catalog of Articles: High Death Rate Reported With Bayers Trasylol
Electrostatic Hazards in the Petroleum Industry pdf ebooks, epub books free download
Electrostatic Hazards in the Petroleum Industry pdf ebooks, epub books free download
Repositorio da Producao Cientifica e Intelectual da Unicamp: Raman spectroscopic monitoring and control of aprotinin...
Repositorio da Producao Cientifica e Intelectual da Unicamp: Raman spectroscopic monitoring and control of aprotinin...
FIT -
Project
Trasylol Drug Injury Lawyers
The mammary gland-specific marsupial ELP and eutherian CTI share a common ancestral gene | BMC Ecology and Evolution | Full Text
The gaussian network model: precise prediction of residue fluctuations and application to binding problems. - Semantic Scholar
Inflammation and infection imaging with a 99mTc-neutrophil elastase in by Mary Rusckowski, Tong Qu et al.
BMRB Entry 4873
Schneeweiss, S., Seeger, J.D., Landon, J. and Walker, A.M. (2008) Aprotinin during Coronary-Artery Bypass Grafting and Risk of...
Gentaur Molecular :SeraLab \ Cynomolgus Monkey Plasma EDTA K2 Individual Donor with Aprotinin, not Filtered \ PK2IA-118-Z
Recombinant Trypsin Inhibitor, Expressed in E.coli, Competitive Serine Protease Inhibitor
Evaluation of the in-house radioimmunoassay method for peptide YY (PYY) | BES2004
A 25% increase in the 2DEG | HSP90 inhibitor|HSP70 Inhibitor
Publications
Neuromics: Transthyretin, αAPP peptides and Alzheimers Disease
Amyloid beta (A4) precursor protein degradation products - Biology-Online
Identification of an aprotinin antiviral domain - Pellegrini - 2001 - FEBS Letters | Herpes Advice
PRODUCTS IN DEVELOPMENT
KIU listed among best teaching health facilities in Uganda - PML Daily
Plus it
HudHud Hub | Ephraim Faircloth Activity Streams
Thrombin Bovine | Natural Proteins | ProSpecbio
Nigravizaĝa emberizo - Vikipedio
Dark Water site
Dependeco (medicino) - Vikipedio
Headshot before after image - Message 10662 by kiu
Best KPI Software - 2018 Reviews, Pricing & Demos
Global Sucrose Polyester Market Professional Survey Report 2019 - Grand Research Store
The Camera Loves Tom Hardy But Tom Has no Love For Them!: ohnotheydidnt - LiveJournal
CU-2010 and CU-2020
... improve coronary endothelial function similarly to aprotinin. Unlike aprotinin, at high doses, their anti- ... CU-2010 and CU-2020 have the ability to inhibit plasmin to a similar extent as aprotinin, but they are 100,000 times better at ... CU-2010 and CU-2020 were developed to avoid many issues associated with the use of aprotinin, including the risk of an allergic ... Since the isolation of aprotinin is expensive, a drug with a simpler synthesis was desired. CU-2010 has a molecular weight of ...
Bayer
Aprotinin and cardiac surgery: a sorry tale of evidence misused". Br J Anaesth. 110 (5): 675-8. doi:10.1093/bja/aet008. PMID ... Trasylol (Aprotinin) Trasylol is a trypsin inhibitor used to control bleeding during major surgery. In a 2006 meeting called by ... Trasylol (aprotinin), used to control bleeding during major surgery, was withdrawn from the market worldwide in 2007 when ... "FDA Revises Labeling for Trasylol (Aprotinin Injection) to Strengthen Safety Warnings and Limit Usage of Drug to Specific ...
Panangipalli Venugopal
"Efficacy of Aprotinin, Epsilon Aminocaproic Acid, or Combination in Cyanotic Heart Disease". Annals of Thoracic Surgery. ...
Ottawa Hospital Research Institute
Paul Hebert discovered that aprotinin is associated with an increased risk of death compared to other drugs routinely used to ... "A comparison of aprotinin and lysine analogues in high-risk cardiac surgery". The New England Journal of Medicine. 358 (22): ...
Fibrin glue
Formulations from different manufacturers may also contain aprotinin, fibronectin, plasminogen, and factor XIII. Factors that ...
SLPI
... eglin c and aprotinin". The Biochemical Journal. 313 (2): 555-60. doi:10.1042/bj3130555. PMC 1216943. PMID 8573092. Kikuchi T, ...
Kallikrein
Prekallikrein Kinin-kallikrein system Kinin Aprotinin List of cutaneous conditions Raspi G (September 1996). "Kallikrein and ...
Kallistatin
"Tissue kallikrein in severe acute pancreatitis in patients treated with high-dose intraperitoneal aprotinin". Pancreas. 19 (4 ...
Pharming (genetics)
In 2004, LSBC announced an agreement with Sigma-Aldritch under which LSB would produce recombinant aprotinin in plants of the ... ProdiGene - was developing several proteins, including aprotinin, trypsin and a veterinary TGE vaccine in corn. Was in process ... Biomanufacturing Press Release Sigma catalog Aprotinin History of bankrupt biotech companies Cordis entry on Novoplant APHIS ... aprotinin, interferon Alpha 2a and 2b, G-CSF, and Hepatitis B vaccine antigens in tobacco. ...
Metascience
Fergusson, Dean; Glass, Kathleen Cranley; Hutton, Brian; Shapiro, Stan (2016). "Randomized controlled trials of aprotinin in ...
Tamulotoxin
Pandey, R; Deshpande, SB (August 2004). "Protective effects of aprotinin on respiratory and cardiac abnormalities induced by ...
Horizontal Environmental Genetic Alteration Agents
June 2010). "Production of pharmaceutical-grade recombinant aprotinin and a monoclonal antibody product using plant-based ...
Thromboelastometry
APTEM test is an EXTEM based assay in which fibrinolysis is inhibited by aprotinin in the reagent. A significant improvement of ...
Glued intraocular lens
0.5 cc of distilled water is then added to thrombin vial whereas aprotinin is mixed with fibrinogen. Both the components are ... aprotinin solution (1,500 KIU in 0.5 ml), one ampoule of sterile water, four 21-gauge needles, two 20-gauge blunt application ...
Fibrinolysis
... fibrinolysis is assessed by comparing the TEM profile in the absence or presence of the fibrinolysis inhibitor aprotinin. ...
Coagulation
Before its withdrawal, aprotinin was used in some forms of major surgery to decrease bleeding risk and the need for blood ...
Knut Aukland
1-78 Repeatable measurement of local and zonal GFR in the rat kidney with aprotinin (sm.m. O. Tenstad og H. E. Williamson), i ...
Myocardial depressant factor
... aprotinin, captopril, imidazole or lidocaine.[citation needed] Myocardial+Depressant+Factor at the US National Library of ...
Kunitz domain
Examples of Kunitz-type protease inhibitors are aprotinin (bovine pancreatic trypsin inhibitor, BPTI), Alzheimer's amyloid ...
Activated clotting time
Clotting time measurements can be affected by drugs such as warfarin, aprotinin, and GpIIb/IIIa inhibitors, and physiologic ...
Collagen, type VI, alpha 3
... salivary proteins and aprotinin type protease inhibitors". The EMBO Journal. 9 (2): 385-93. doi:10.1002/j.1460-2075.1990. ...
Tendinopathy
... aprotinin, polysulphated glycosaminoglycan, skin derived fibroblasts etc.) for treating Achilles tendinopathy. As of 2014 there ...
ATC code B02
B02AA01 Aminocaproic acid B02AA02 Tranexamic acid B02AA03 Aminomethylbenzoic acid B02AB01 Aprotinin B02AB02 Alfa1 antitrypsin ...
LRP1
... has been shown to interact with: A2-Macroglobulin, β-amyloid precursor protein, APBB1, APOE, Aprotinin, C1S/C1q inhibitor ...
List of drugs: An-Ap
... aprotinin (INN) aptazapine (INN) aptiganel (INN) aptocaine (INN) (Articles with short description, Short description is ...
Protease inhibitor (biology)
Aprotinin Bestatin Calpain inhibitor I and II Chymostatin E-64 Leupeptin (N-acetyl-L-leucyl-L-leucyl-L-argininal) alpha-2- ...
Lysis buffer
... such as aprotinin SDS is ionic denaturing detergent. Hot SDS buffer is often used when the proteins need to be completely ...
Enver Duran
The Importance of Aprotinin and Pentoxifylline in Preventing the Leucocyte Sequestration and Lung İnjury Caused By Protamine at ...
Radioimmunoprecipitation assay buffer
... aprotinin, pepstatin, 1-5 mM EDTA, 0.5-1 mM EGTA, 5 mM aminocaproic acid) or commercial protease inhibitor cocktail (use ...
Hyperfibrinolysis
Since the use of aprotinin has been abandoned due to major side effects, the treatment or prophylaxis of hyperfibrinolysis is ... The APTEM test, a tissue factor activated, heparin insensitive test performed in the presence of aprotinin (fibrinolysis ... but without aprotinin). A normalization or improvement of the TEMogram in APTEM versus EXTEM confirms hyperfibrinolysis. This ...
Aprotinin - Wikipedia
Nordic became distributor of aprotinin in 2012. Aprotinin is a monomeric (single-chain) globular polypeptide derived from ... aprotinin may increase the risk of death. On October 29, 2006 the Food and Drug Administration issued a warning that aprotinin ... 30,000 of whom received aprotinin and the rest other anti-fibrinolytics. The study concluded aprotinin carried greater risks. ... Small amounts of aprotinin can be added to tubes of drawn blood to enable laboratory measurement of certain rapidly degraded ...
Search Results: aprotinin bovine, Rash or multiple lesions
Aprotinin | Profiles RNS
"Aprotinin" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Aprotinin" by people in this website by year, and whether " ... Long-term mortality associated with aprotinin following coronary artery bypass graft surgery. JAMA. 2007 Jun 13; 297(22):2476-7 ... Below are the most recent publications written about "Aprotinin" by people in Profiles. ...
Room B, 10/17/2000 2: 00 PM - 4: 00 PM (PS) Aprotinin Use in Patients Taking Inhibitors of Angiotensin Converting Enzyme (ACEI)...
Transfusion Requirements in Liver Transplantation: Overview, History and Background, Bleeding During Liver Transplantation
These include aprotinin, epsilon amino caproic acid (epsilon-ACA), and tranexamic acid. [53] Recently, aprotinin has incited ... Aprotinin. Aprotinin is a serine protease inhibitor that prevents the lysis of fibrinogen by inhibiting plasmin, kallikrein, ... However, other studies have not reported a benefit with aprotinin use. Therefore, the precise role of aprotinin remains ... A comparison of aprotinin and lysine analogues in high-risk cardiac surgery. N Engl J Med. 2008 May 29. 358(22):2319-31. [QxMD ...
Transfusion Requirements in Liver Transplantation: Overview, History and Background, Bleeding During Liver Transplantation
These include aprotinin, epsilon amino caproic acid (epsilon-ACA), and tranexamic acid. [53] Recently, aprotinin has incited ... Aprotinin. Aprotinin is a serine protease inhibitor that prevents the lysis of fibrinogen by inhibiting plasmin, kallikrein, ... However, other studies have not reported a benefit with aprotinin use. Therefore, the precise role of aprotinin remains ... A comparison of aprotinin and lysine analogues in high-risk cardiac surgery. N Engl J Med. 2008 May 29. 358(22):2319-31. [QxMD ...
A-Z Drug Index for Prescription and OTC Medications
IMSEAR at SEARO: Comparison of three dose regimens of aprotinin in infants undergoing the arterial switch operation.
Aprotinin dosage regimen of 40,000 KIU/kg at induction, in CPB prime and postoperatively for three hours was most effective in ... Group I (n = 8) patients received aprotinin in a dose of 20,000 kallikrein inhibiting units (KIU)/kg after induction of ... To determine the most effective dose regimen of aprotinin for infants undergoing arterial switch operation for transposition of ... Comparison of three dose regimens of aprotinin in infants undergoing the arterial switch operation. ...
Haute Autorité de Santé - Résultat de recherche
Aprotinin in lung transplantation is associated with an increased incidence of primary graft dysfunction - Fingerprint -...
Creatina: MedlinePlus suplementos
Natural Proteins | Recombinant Proteins | Adooq Bioscience
Aprotinin reduces operative closure time and blood product use after pediatric bypass - Fingerprint - Johns Hopkins...
Martin Michaelis - Search Results - PubMed
A new method to measure renal tubular degradation of small filtered proteins in man using radiolabelled aprotinin (Trasylol) |...
2. Aprotinin (0.5 or 5.0 mg) was labelled with either 99mTc (40 MBq) or 131I (1 MBq) and injected intravenously in 19 renal ... A new method to measure renal tubular degradation of small filtered proteins in man using radiolabelled aprotinin (Trasylol) R ... Chromatography showed all plasma activity as undegraded aprotinin and urine activity only as the free labels (99mTcO-4 or 131I- ... 6. Fractional renal tubular degradation of 99mTc-aprotinin (in h−1) is a measure of its turnover value over a given interval ...
Intraoperative anaphylaxis due to aprotinin after local application of fibrin sealant diagnosed by skin tests and basophil...
2 and pure aprotinin demonstrated that the causative agent was likely aprotinin. We diagnosed aprotinin-induced anaphylaxis ... 2, whose main component is aprotinin. Subsequently, basophil activation tests using fibrin sealant vial no. ... From: Intraoperative anaphylaxis due to aprotinin after local application of fibrin sealant diagnosed by skin tests and ...
SNRI
Aprotinin reduces interleukin-8 production and lung neutrophil accumulation after cardiopulmonary bypass<...
Since aprotinin reduces TNF release during CPB, the effects of aprotinin on BALF IL-8 concentrations and neutrophil levels were ... Since aprotinin reduces TNF release during CPB, the effects of aprotinin on BALF IL-8 concentrations and neutrophil levels were ... Since aprotinin reduces TNF release during CPB, the effects of aprotinin on BALF IL-8 concentrations and neutrophil levels were ... Since aprotinin reduces TNF release during CPB, the effects of aprotinin on BALF IL-8 concentrations and neutrophil levels were ...
Metabolites | Free Full-Text | Targeting the Pentose Phosphate Pathway for SARS-CoV-2 Therapy
Heparin dosing, indications, interactions, adverse effects, and more
Mercury in PDB 3ldi: Crystal Structure of Aprotinin in Complex with Sucrose Octasulfate: Unusual Interactions and Implication...
Crystal Structure of Aprotinin in Complex with Sucrose Octasulfate: Unusual Interactions and Implication For Heparin Binding ... I.S.Yang, T.G.Kim, B.S.Park, K.J.Cho, J.-H.Lee, Y.Park, K.H.Kim. Crystal Structures of Aprotinin and Its Complex with Sucrose ... A full contact list of Mercury with other atoms in the Hg binding site number 1 of Crystal Structure of Aprotinin in Complex ... A full contact list of Mercury with other atoms in the Hg binding site number 2 of Crystal Structure of Aprotinin in Complex ...
Urinary aprotinin as a predictor of acute kidney injury after cardiac surgery in children receiving aprotinin therapy<...
Urinary aprotinin as a predictor of acute kidney injury after cardiac surgery in children receiving aprotinin therapy. In: ... Urinary aprotinin as a predictor of acute kidney injury after cardiac surgery in children receiving aprotinin therapy. / Nguyen ... Urinary aprotinin as a predictor of acute kidney injury after cardiac surgery in children receiving aprotinin therapy. ... Urinary aprotinin as a predictor of acute kidney injury after cardiac surgery in children receiving aprotinin therapy. ...
QIAexpress Type IV Kit
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TachoSil (fibrin sealant) dose, indications, adverse effects, interactions... from PDR.net
Aprotinin hypersensitivity. Fibrin sealant, human is contraindicated in patients known to have anaphylactic or severe systemic ... Artiss and Tisseel also contain aprotinin, which has been shown to increase resistance of the fibrin sealant clot to ... if systemic aprotinin has been previously administered. Even if the first treatment was well tolerated, a subsequent ... Artiss and Tisseel are contraindicated in patients with known aprotinin hypersensitivity.[41169] [41172] Hypersensitivity or ...
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LeupeptinTrasylolCardiac surgeryPMSFThrombinAEBSFTranexamicTrypsin InhibitorProtease inhibitorsTransfusion requirementsInsulinPeptidesProteasesProteinBovineReperfusionRenalTLCKMortalityDegradationLungEDTA30,000KallikreinConcentrationsBlood lossPotentPatientsWidelyParametersSerumTissueTubesDoseRatsCautionResultsDrugStudyEffectsPlasmaMajorTrialLevels
Leupeptin3
- five mM phenylmethylsulfonyl fluoride, 1uM leupeptin, 1uM aprotinin. (pkc-inhibitors.com)
- 12. Protease inhibitors: Leupeptin(10mg/ml stock), Aprotinin(10mg/ml stock), Pepstatin A (1mg/ml stock), phenylmethylsulfonyl fluoride (PMSF, 35mg/ml stock). (researchsquare.com)
- Immunoblotting analysis Cell lysates were prepared using RIPA buffer [50?mm Tris, 0.15?m NaCl, 1% Triton X\100, 1% sodium deoxycholate, 0.1% SDS (sodium dodecyl sulfate), 1?mm EDTA (ethylenediaminetetraacetic acid), Ibodutant (MEN 15596) 1?mm Na orthovanadate, 1?mm PMSF (phenylmethylsulfonyl fluoride), 25?gmL?1 leupeptin, and 25?gmL?1 aprotinin]. (recob-tlse.org)
Trasylol7
- The drug aprotinin (Trasylol, previously Bayer and now Nordic Group pharmaceuticals), is a small protein bovine pancreatic trypsin inhibitor (BPTI), or basic trypsin inhibitor of bovine pancreas, which is an antifibrinolytic molecule that inhibits trypsin and related proteolytic enzymes. (wikipedia.org)
- Under the trade name Trasylol, aprotinin was used as a medication administered by injection to reduce bleeding during complex surgery, such as heart and liver surgery. (wikipedia.org)
- 1. A method has been developed to measure the renal tubular degradation of small filtered proteins in man using radiolabelled aprotinin (Trasylol), a 6500 Da cationic polypeptide. (silverchair.com)
- There was one blot on the sales sheet, however, with the performance of Trasylol (aprotinin), which is used to reduce blood loss during heart surgery. (pharmatimes.com)
- The FDA has approved revised labeling for Trasylol (aprotinin injection), from Bayer Pharmaceuticals, West Haven, Conn., (800) 468-0894. (drugtopics.com)
- Bayer announced new guidance to physicians and health care providers regarding the use of Trasylol® (aprotinin injection) in patients at an increased risk of blood loss and blood transfusion undergoing coronary artery bypass graft (CABG) surgery with cardiopulmonary bypass (CPB). (worldpharmanews.com)
- Leverkusen, Germany and West Haven, CT (Nov. 5)- Following consultation with the German Federal Institute for Drugs and Medical Devices (BfArM), the US Food and Drug Administration, Health Canada, and other health authorities, Bayer has elected to temporarily suspend worldwide marketing of "Trasylol" (aprotinin injection) until final results from the Canadian BART trial can be compiled, received and evaluated. (pharmtech.com)
Cardiac surgery3
- In cardiac surgery with a high risk of significant blood loss, aprotinin significantly reduced bleeding, mortality and hospital stay. (wikipedia.org)
- A comparison of aprotinin and lysine analogues in high-risk cardiac surgery. (ohri.ca)
- Antibody formation after drug administration during cardiac surgery: parameters for aprotinin use. (pneumotox.com)
PMSF1
- Next, we examined whether this uncommon effect could possibly be obstructed by addition of a number of protease inhibitors (2% fetal leg serum, 125 M Quizartinib inhibitor database aprotinin, 1 mM AEBSF, 1 mM PMSF, 5 mg/mL 6-aminohexanoic acidity, 100 M antipain, 4 mM benzamidine HCl, 10 M E-64, 1 mM isn't enough to stabilize HoxA9. (shahmnil-mgh-harvard.org)
Thrombin2
- The second component contains thrombin, calcium chloride , and antifibrinolytic agents such as aprotinin. (dictionary.net)
- Lower concentrations of thrombin resulted in equivalent neuroprotection as the antifibrinolytic, aprotinin, and the direct thrombin inhibitor, argatroban. (emory.edu)
AEBSF1
- AEBSF E-64 Aprotinin EDTA Leupeptine Store -20°C Lyophillzed blend of five protease inhibitors for applications that require animal free reagents. (thomassci.com)
Tranexamic2
- One trial on Aprotinin (1982) and two trials on Tranexamic acid (2010 and 2013) were included. (sabm.org)
- These include aprotinin, tranexamic acid (TXA), and epsilon aminocaproic acid (EACA, trade name Amicar). (ijssurgery.com)
Trypsin Inhibitor1
- Loss of epidermal cohesion in normal skin explants incubated with pemphigus foliaceous IgG was dependent upon the addition of plasminogen and was inhibited by aprotinin or lima bean trypsin inhibitor, which indicated that plasmin is the active enzyme in producing acantholysis. (cdc.gov)
Protease inhibitors1
- The protease inhibitors aprotinin and ilomastat and the antioxidant trolox were evaluated for efficacy in ameliorating SM-induced lung injury. (dtic.mil)
Transfusion requirements1
- Aprotinin dosage regimen of 40,000 KIU/kg at induction, in CPB prime and postoperatively for three hours was most effective in reducing postoperative blood loss and PRBC transfusion requirements. (who.int)
Insulin1
- Effects of Aprotinin and Insulin on Regulation of Inducible Nitric Oxide Synthase m-RNA in the Mouse Macrophage. (ekja.org)
Peptides1
- We determined that aprotinin and D-arginine-based 9-12-mer peptides are potent inhibitors of WNV NS3 with K i values of 26 nM and 1 nM respectively. (elsevier.com)
Proteases1
- citation needed] Aprotinin is a competitive inhibitor of several serine proteases, specifically trypsin, chymotrypsin and plasmin at a concentration of about 125,000 IU/ml, and kallikrein at 300,000 IU/ml. (wikipedia.org)
Protein3
- The protein was identified as aprotinin. (wustl.edu)
- The dose is lowered protein synthesis the MMP-inhibitor aprotinin. (thesalarmy.com)
- A low protein C activity is expected in aprotinin treated patients because aprotinin is an inhibitor of activated protein C. Oral anticoagulant therapy interferes with the formation of g-carboxyglutamic acid moiety of the protein C molecules during biosynthesis in the liver, which results in a loss of anticoagulant activity. (diapharma.com)
Bovine1
- Aprotinin is a monomeric (single-chain) globular polypeptide derived from bovine lung tissue. (wikipedia.org)
Reperfusion1
- 15. Aprotinin ameliorates ischemia/reperfusion injury in a rat hind limb model. (kursatbozkurt.com)
Renal4
- Aprotinin does not have any adverse effect on renal function. (who.int)
- 2. Aprotinin (0.5 or 5.0 mg) was labelled with either 99m Tc (40 MBq) or 131 I (1 MBq) and injected intravenously in 19 renal patients (10 with normal renal function and nine on haemodialysis). (silverchair.com)
- Activity in plasma and urine was measured over 48 h, and chromatography with Sephadex-G-25-M was used to separate labelled aprotinin from free 99m TcO - 4 or 131 I - . Renal uptake was measured for 99m Tc-labelled aprotinin only. (silverchair.com)
- 6. Serine protease inhibitor aprotinin ameliorates renal injury in a rat model of ischemia-perfusion injury. (kursatbozkurt.com)
TLCK1
- 2) the supernatant of a human bronchial epithelial cell culture line, A549, after 24 h of TNF stimulation with or without aprotinin or N-α-tosyl- L-lysine chloromethyl ketone (TLCK) (a potent protease inhibitor), and 3) BALF. (elsevier.com)
Mortality2
- Long-term mortality associated with aprotinin following coronary artery bypass graft surgery. (umassmed.edu)
- In contrast, aprotinin and extended-release metoprolol might increase mortality. (elsevier.com)
Degradation1
- To protect rhIGF-I from degradation in the small-intestinal contents, the effect of some adjuvants was examined and their degradation was markedly inhibited by the presence of aprotinin or casein. (aspetjournals.org)
Lung2
- These results demonstrate that aprotinin blunts IL-8 production and reduces neutrophil lung accumulation post-CPB. (elsevier.com)
- Effects of Aprotinin on Acute Lung Injury by Endotoxin in Rabbits. (ekja.org)
EDTA1
- Venous blood samples were collected mined by commercially available radio- using plain tubes for routine tests and chilled immunoassay kits for met-enkephalin, polyethylene tubes containing aprotinin -endorphin and leu-enkephalin (Penin- and/or ethylenediaminetetra-acetic acid sula Laboratories Inc, Division of Bachem, (EDTA). (who.int)
30,0001
- In September 2006, Bayer A.G. was faulted by the FDA for not revealing during testimony the existence of a commissioned retrospective study of 67,000 patients, 30,000 of whom received aprotinin and the rest other anti-fibrinolytics. (wikipedia.org)
Kallikrein1
- The kallikrein-like enzyme has been purified from homogenates of the kidney and aorta by a DEAE-Sepharose and aprotinin affinity columnn. (nii.ac.jp)
Concentrations1
- Since aprotinin reduces TNF release during CPB, the effects of aprotinin on BALF IL-8 concentrations and neutrophil levels were determined after CPB in adult humans. (elsevier.com)
Blood loss2
- To determine the most effective dose regimen of aprotinin for infants undergoing arterial switch operation for transposition of the great arteries in reducing blood loss and postoperative packed red blood cell (PRBC) requirements. (who.int)
- Reduction of blood loss using high-dose aprotinin in major orthopaedic surgery: a prospective, double-blind, randomised and placebo-controlled study. (jeserschek.at)
Potent1
- In AP-test fibrinolysis is inhibited in-vitro using aprotinin, a potent direct antagonist of plasmin, the effector protease of fibrinolysis. (avant-medical.com)
Patients5
- Study patients were equally divided into a control group (n = 8, Group 1) and an aprotinin-treated group (n = 8, Group 2). (elsevier.com)
- The most popular drug, aprotinin, was widely assumed to be the best, but to everyone s surprise, BART revealed that patients who received aprotinin were actually 50 per cent more likely to die than patients who received either of the other two drugs. (ohri.ca)
- The research showed of 108 patients who died, the proportion who were believed to have died of cardiogenic shock, right ventricular failure, congestive heart failure or myocardial infarction (heart attack) was higher in the aprotinin group than in the other two groups. (irwinmitchell.com)
- The Aprotinin trial included 77 patients, one TXA study had 240 traumatic brain injury patients, the other 20,211 trauma patients from 274 hospitals in 40 countries. (sabm.org)
- To exclude that aprotinin as possible anti-inflammatory agent was the eliciting factor in endogenous alkaline phosphatase release we performed a small study in patients undergoing CABG with the use of CPB, but without the use of aprotinin. (uu.nl)
Widely1
- Traysol, also known by its generic name aprotinin, has been widely used since the 1990s during heart bypass surgery to reduce bleeding. (irwinmitchell.com)
Parameters1
- Treatment with aprotinin or ilomastat eliminated these PF changes, yielding results comparable with controls for each of these parameters. (dtic.mil)
Serum1
- The 2 h urinary aprotinin level correlated with serum creatinine, duration of AKI, and length of hospital stay. (wustl.edu)
Tissue1
- 0.3 μM aprotinin) and homogenized using a turrax tissue homogenizer (Marconi, Piracicaba, Brazil). (sirtsignaling.com)
Tubes1
- At 60 min after the infusion of galanin or vehicle, animals were decapitated and trunk blood was collected in plastic tubes containing 1000 IU aprotinin (inhibitor of protease) per each ml of blood. (nel.edu)
Dose2
- IMSEAR at SEARO: Comparison of three dose regimens of aprotinin in infants undergoing the arterial switch operation. (who.int)
- Verma Yashwant S, Chauhan Sandeep, Bisoi Akshay K, Gharde Parag, Kiran Usha, Das Sambhu N. Comparison of three dose regimens of aprotinin in infants undergoing the arterial switch operation. (who.int)
Rats1
- At 1 min before the exposure rats were treated with one of the following intravenous aprotinin, 4.4 mgkg intraperitoneal ip ilomastat, 25 mgkg or ip trolox, 500 microgramskg. (dtic.mil)
Caution1
- 2 μg/ml Aprotinin (CAUTION! (koko.gov.my)
Results1
- When the results were announced, the manufacturer suspended marketing of aprotinin and heart surgery changed, virtually overnight. (ohri.ca)
Drug1
- The study has saved thousands of lives and likely millions of dollars, as aprotinin was actually the most expensive drug by far. (ohri.ca)
Study2
- In this study it is demonstrated that the release of endogenous alkaline phosphatase is not induced by aprotinin, and that it is only induced by bolus bIAP followed by continuous infusion. (uu.nl)
- Samples were taken from the people involved in the study to the tube containing aprotinin. (fusabil.org)
Effects1
- The risk or severity of adverse effects can be increased when Aprotinin is combined with Carbamoylcholine. (drugbank.com)
Plasma1
- The use of injectable substances such as platelet-rich plasma, autologous blood, polidocanol, corticosteroids and aprotinin in and around tendons is popular for Achilles tendinopathy, but evidence to support their role is minimal [ 3 ]. (springer.com)
Major1
- This graph shows the total number of publications written about "Aprotinin" by people in this website by year, and whether "Aprotinin" was a major or minor topic of these publications. (umassmed.edu)
Trial1
- The quality of evidence from the TXA trials were considered very high, that from the Aprotinin trial moderate. (sabm.org)
Levels2
- Urinary aprotinin levels 2 h after initiation of CPB were predictive of AKI (for functional assay: 92% sensitivity, 96% specificity, area under the curve of 0.98). (wustl.edu)
- We concluded that urinary aprotinin levels 2 h after initiation of CPB predict the development of AKI and adverse clinical outcomes. (wustl.edu)