Triticum: A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.DNA Fragmentation: Splitting the DNA into shorter pieces by endonucleolytic DNA CLEAVAGE at multiple sites. It includes the internucleosomal DNA fragmentation, which along with chromatin condensation, are considered to be the hallmarks of APOPTOSIS.Caspases: A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Cell Death: The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.Iodobenzoates: Benzoic acid esters or salts substituted with one or more iodine atoms.Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Inflammation: A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.Peroxidase: A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 1.11.1.7.Lactoferrin: An iron-binding protein that was originally characterized as a milk protein. It is widely distributed in secretory fluids and is found in the neutrophilic granules of LEUKOCYTES. The N-terminal part of lactoferrin possesses a serine protease which functions to inactivate the TYPE III SECRETION SYSTEM used by bacteria to export virulence proteins for host cell invasion.Cytoplasmic Granules: Condensed areas of cellular material that may be bounded by a membrane.Neutrophil Infiltration: The diffusion or accumulation of neutrophils in tissues or cells in response to a wide variety of substances released at the sites of inflammatory reactions.Subarachnoid Hemorrhage: Bleeding into the intracranial or spinal SUBARACHNOID SPACE, most resulting from INTRACRANIAL ANEURYSM rupture. It can occur after traumatic injuries (SUBARACHNOID HEMORRHAGE, TRAUMATIC). Clinical features include HEADACHE; NAUSEA; VOMITING, nuchal rigidity, variable neurological deficits and reduced mental status.Neurosciences: The scientific disciplines concerned with the embryology, anatomy, physiology, biochemistry, pharmacology, etc., of the nervous system.Vasospasm, Intracranial: Constriction of arteries in the SKULL due to sudden, sharp, and often persistent smooth muscle contraction in blood vessels. Intracranial vasospasm results in reduced vessel lumen caliber, restricted blood flow to the brain, and BRAIN ISCHEMIA that may lead to hypoxic-ischemic brain injury (HYPOXIA-ISCHEMIA, BRAIN).Carotid Artery, Internal: Branch of the common carotid artery which supplies the anterior part of the brain, the eye and its appendages, the forehead and nose.Zinc: A metallic element of atomic number 30 and atomic weight 65.38. It is a necessary trace element in the diet, forming an essential part of many enzymes, and playing an important role in protein synthesis and in cell division. Zinc deficiency is associated with ANEMIA, short stature, HYPOGONADISM, impaired WOUND HEALING, and geophagia. It is known by the symbol Zn.Subarachnoid Space: The space between the arachnoid membrane and PIA MATER, filled with CEREBROSPINAL FLUID. It contains large blood vessels that supply the BRAIN and SPINAL CORD.Neuroprotective Agents: Drugs intended to prevent damage to the brain or spinal cord from ischemia, stroke, convulsions, or trauma. Some must be administered before the event, but others may be effective for some time after. They act by a variety of mechanisms, but often directly or indirectly minimize the damage produced by endogenous excitatory amino acids.HMGB1 Protein: A 24-kDa HMGB protein that binds to and distorts the minor grove of DNA.HMGB2 Protein: A 23-kDa HMG-box protein that binds to and distorts the minor grove of DNA.High Mobility Group Proteins: A family of low-molecular weight, non-histone proteins found in chromatin.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)HMGA1a Protein: An 11-kDa AT-hook motif-containing (AT-HOOK MOTIFS) protein that binds to the minor grove of AT-rich regions of DNA. It is the full-length product of the alternatively-spliced HMGA1 gene and may function as an architectural chromatin binding protein that is involved in transcriptional regulation.Macrophage Activation: The process of altering the morphology and functional activity of macrophages so that they become avidly phagocytic. It is initiated by lymphokines, such as the macrophage activation factor (MAF) and the macrophage migration-inhibitory factor (MMIF), immune complexes, C3b, and various peptides, polysaccharides, and immunologic adjuvants.Macrophages, Peritoneal: Mononuclear phagocytes derived from bone marrow precursors but resident in the peritoneum.Staurosporine: An indolocarbazole that is a potent PROTEIN KINASE C inhibitor which enhances cAMP-mediated responses in human neuroblastoma cells. (Biochem Biophys Res Commun 1995;214(3):1114-20)Lovastatin: A fungal metabolite isolated from cultures of Aspergillus terreus. The compound is a potent anticholesteremic agent. It inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase (HYDROXYMETHYLGLUTARYL COA REDUCTASES), which is the rate-limiting enzyme in cholesterol biosynthesis. It also stimulates the production of low-density lipoprotein receptors in the liver.Polyisoprenyl Phosphates: Phosphoric or pyrophosphoric acid esters of polyisoprenoids.Mevalonic AcidKidney Tubules, Proximal: The renal tubule portion that extends from the BOWMAN CAPSULE in the KIDNEY CORTEX into the KIDNEY MEDULLA. The proximal tubule consists of a convoluted proximal segment in the cortex, and a distal straight segment descending into the medulla where it forms the U-shaped LOOP OF HENLE.Carcinoma, Renal Cell: A heterogeneous group of sporadic or hereditary carcinoma derived from cells of the KIDNEYS. There are several subtypes including the clear cells, the papillary, the chromophobe, the collecting duct, the spindle cells (sarcomatoid), or mixed cell-type carcinoma.Hycanthone: Potentially toxic, but effective antischistosomal agent, it is a metabolite of LUCANTHONE.Serpins: A family of serine proteinase inhibitors which are similar in amino acid sequence and mechanism of inhibition, but differ in their specificity toward proteolytic enzymes. This family includes alpha 1-antitrypsin, angiotensinogen, ovalbumin, antiplasmin, alpha 1-antichymotrypsin, thyroxine-binding protein, complement 1 inactivators, antithrombin III, heparin cofactor II, plasminogen inactivators, gene Y protein, placental plasminogen activator inhibitor, and barley Z protein. Some members of the serpin family may be substrates rather than inhibitors of SERINE ENDOPEPTIDASES, and some serpins occur in plants where their function is not known.Tilorone: An antiviral agent used as its hydrochloride. It is the first recognized synthetic, low-molecular-weight compound that is an orally active interferon inducer, and is also reported to have antineoplastic and anti-inflammatory actions.bcl-2 Homologous Antagonist-Killer Protein: A multi-domain mitochondrial membrane protein and member of the bcl-2 Protein family. Bak protein interacts with TUMOR SUPPRESSOR PROTEIN P53 and promotes APOPTOSIS.Uracil Mustard: Nitrogen mustard derivative of URACIL. It is a alkylating antineoplastic agent that is used in lymphatic malignancies, and causes mainly gastrointestinal and bone marrow damage.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Cocaine: An alkaloid ester extracted from the leaves of plants including coca. It is a local anesthetic and vasoconstrictor and is clinically used for that purpose, particularly in the eye, ear, nose, and throat. It also has powerful central nervous system effects similar to the amphetamines and is a drug of abuse. Cocaine, like amphetamines, acts by multiple mechanisms on brain catecholaminergic neurons; the mechanism of its reinforcing effects is thought to involve inhibition of dopamine uptake.Neurons: The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.Cocaine-Related Disorders: Disorders related or resulting from use of cocaine.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Brain: The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)DNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.Social Sciences: Disciplines concerned with the interrelationships of individuals in a social environment including social organizations and institutions. Includes Sociology and Anthropology.Micropore Filters: A membrane or barrier with micrometer sized pores used for separation purification processes.In Situ Nick-End Labeling: An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.

*MUL1

... and fragmentation of the DNA and nucleus. This is followed by fragmentation into apoptotic bodies that are quickly removed by ... where it regulates mitochondrial morphology and apoptosis. Specifically, this enzyme has pro-apoptotic functions. An apoptotic ... Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial ... Can also activate NF-κB to initiate mitochondria-to-nucleus signaling under stress. As aforementioned, MUL1 encodes for an ...

*Apoptosis

Nagata S (April 2000). "Apoptotic DNA fragmentation". Exp. Cell Res. 256 (1): 12-8. doi:10.1006/excr.2000.4834. PMID 10739646. ... The nucleus breaks into several discrete chromatin bodies or nucleosomal units due to the degradation of DNA. Apoptosis ... In order to perform analysis of apoptotic versus necrotic (necroptotic) cells, one can do analysis of morphology by time-lapse ... Anoikis Apaf-1 Apo2.7 Apoptotic DNA fragmentation Atromentin induces apoptosis in human leukemia U937 cells. Autolysis ...

*Programmed cell death

Anoikis Apoptosis-inducing factor Apoptosis versus Pseudoapoptosis Apoptosome Apoptotic DNA fragmentation Autolysis (biology) ... There appears to be some variation in the morphology and indeed the biochemistry of these suicide pathways; some treading the ... trigeminal nucleus, cerebellum, and spinal cord. However, PCD of neurons due to Bax deletion or Bcl-2 overexpression does not ... These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, and chromosomal DNA ...

*Cell death

These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, and chromosomal DNA ... There appears to be some variation in the morphology and indeed the biochemistry of these suicide pathways; some treading the ... Called "non-apoptotic programmed cell-death" (or "caspase-independent programmed cell-death" or "necroptosis"), these ... The process is characterized by mitochondrial swelling, cytoplasm vacuolization, and swelling of the nucleus and cytoplasm. ...

*Apoptosis

Nagata S (April 2000). "Apoptotic DNA fragmentation". Experimental Cell Research. 256 (1): 12-8. doi:10.1006/excr.2000.4834. ... The nucleus breaks into several discrete chromatin bodies or nucleosomal units due to the degradation of DNA.[53] ... In order to perform analysis of apoptotic versus necrotic (necroptotic) cells, one can do analysis of morphology by time-lapse ... These include absence of apoptotic markers (caspase activation, cytochrome c release, and oligonucleosomal DNA fragmentation) ...

*Parthanatos

AIF is then translocated to the nucleus where it induces chromatin condensation and large scale (50Kb) DNA fragmentation. How ... apoptosis only produces small scale fragmentation) and does not form apoptotic bodies. While parthanatos does share ... Increased production of reactive oxygen species in hyperglycemic conditions requires dynamic change of mitochondrial morphology ... Human AIF have a DNA binding site that would indicate that AIF binds directly to the DNA in the nucleus directly causing the ...

*Paraptosis

In apoptosis, HMGB1, a chromatin protein, is retained within the nucleus to result in formations of apoptotic bodies, while in ... This then caused cells to undergo programmed cell death with considerable vacuolization and an absence of DNA fragmentation and ... and lack of apoptotic morphology. Paraptosis lacks several of the hallmark characteristics of apoptosis, such as membrane ... Paraptosis does not demonstrate nuclear fragmentation, formation of apoptotic bodies, or definitive demonstration of chromatin ...

*Poly(amidoamine)

... and nucleus morphology (DAPI staining). However, in other cell lines, the MTT assay and several other assays revealed some ... This could result in a PAMAM-DNA complex, which would make DNA transfection more efficient due to neutralization of the charges ... The fragmentation of some branches of the dendrimer is thought to loosen up the overall structure (fewer amide bonds and space ... "Dendrimer-Based Targeted Delivery of an Apoptotic Sensor in Cancer Cells". Biomacromolecules. 8 (1): 13-8. doi:10.1021/ ...

*Circulating tumor cell

DNA and RNA assays, and all other relevant molecular techniques using DNA and RNA. When circulating tumor cells are captured ... Apoptotic CTCs are traditional CTCs that are undergoing apoptosis (cell death): Epic Sciences technology identifies nuclear ... fragmentation or cytoplasmic blebbing associated with apoptosis. Measuring the ratio of traditional CTC to apoptotic CTCs - ... This method involves technology to separate nucleated cells from red blood cells, which lack a nucleus. All nucleated cells, ...
Synthetic hexaploid wheats (SHWs) are an important component of the breeding programs of the International Maize and Wheat Improvement Center (CIMMYT). CIMMYT germplasm is the most important source of genetic resources for wheat breeding programs in Iran, but their utilization has to be examined prior to their incorporation into breeding programs. This study was conducted to evaluate the resistance of 128 CIMMYT SHWs to septoria tritici blotch (STB), a destructive wheat disease caused by Zymoseptoria tritici. Wheat seedlings were inoculated with Z. tritici spores in the greenhouse, kept at 20-22ºC and scored 21 days post inoculation by estimating the percentage of necrotic lesions bearing pycnidia. Inter simple sequence repeat (ISSR) markers revealed that 11 Z. tritici isolates had high genetic variability. The isolates varied in their virulence towards SHWs. Among all interactions (n=1408), 304 resistance responses were identified. Of 128 genotypes, 60 synthetic hexaploid wheat genotypes showed no
The disomic addition lines of chromosomes 4R and 6R of Secale cereale cv. Imperial rye in Triticum aestivum cv. Chinese Spring were completely free from Karnal bunt disease of wheat under artificial inoculation. The rye chromosomes 4R and 6R were transferred into a high yielding but Karnal bunt susceptible bread wheat variety WL711 through backcrossing. The monosomic and disomic addition lines of 4R and 6R in WL711 also maintained resistance against a particular isolate of Karnal bunt during backcrossing whereas their euploid segregants were as susceptible as the recurrent parent. The 4R and 6R addition lines, however, were susceptible to a new isolate of Neovossia indica virulent on triticale. The work to substitute the rye chromosomes for their B and D wheat genome homoeologues is in progress.. ...
Emmer wheat as the progenitor of common wheat, holds the genetic potentiality for improvement of wheat yield, quality and stress tolerance such as drought and salt. To comprehensively evaluate the salt tolerance of emmer wheat, a total of 30 traits including growth, physiology and photosynthesis related as well as K+ and Na+ content of 30 wild emmer and 14 durum wheat accessions were systematically investigated and compared between normal and saline conditions. Salt tolerance index (STI) based on multiple regression analysis of these traits was calculated and five wild emmer accessions showed high salt tolerance, which could be used as valuable resource for wheat salt tolerance improvement. Furthermore, wild emmer genotypes showed wider trait performance variation compared to durum wheat, indicating the higher genetic diversity in wild emmer wheat. Then, shoot Na+ content, shoot K+/Na+ ratio, root length and root surface area were identified as suitable indexes for salt tolerance evaluation. Na+
Background: Triticum monococcum (2n) is a close ancestor of T. urartu, the A-genome progenitor of cultivated hexaploid wheat, and is therefore a useful model for the study of components regulating photomorphogenesis in diploid wheat. In order to develop genetic and genomic resources for such a study, we constructed genome-wide transcriptomes of two Triticum monococcum subspecies, the wild winter wheat T. monococcum ssp. aegilopoides (accession G3116) and the domesticated spring wheat T. monococcum ssp. monococcum (accession DV92) by generating de novo assemblies of RNA-Seq data derived from both etiolated and green seedlings. Principal Findings: The de novo transcriptome assemblies of DV92 and G3116 represent 120,911 and 117,969 transcripts, respectively. We successfully mapped ~90% of these transcripts from each accession to barley and ~95% of the transcripts to T. urartu genomes. However, only ~77% transcripts mapped to the annotated barley genes and ~85% transcripts mapped to the annotated T. ...
Background: Triticum monococcum (2n) is a close ancestor of T. urartu, the A-genome progenitor of cultivated hexaploid wheat, and is therefore a useful model for the study of components regulating photomorphogenesis in diploid wheat. In order to develop genetic and genomic resources for such a study, we constructed genome-wide transcriptomes of two Triticum monococcum subspecies, the wild winter wheat T. monococcum ssp. aegilopoides (accession G3116) and the domesticated spring wheat T. monococcum ssp. monococcum (accession DV92) by generating de novo assemblies of RNA-Seq data derived from both etiolated and green seedlings. Principal Findings: The de novo transcriptome assemblies of DV92 and G3116 represent 120,911 and 117,969 transcripts, respectively. We successfully mapped ~90% of these transcripts from each accession to barley and ~95% of the transcripts to T. urartu genomes. However, only ~77% transcripts mapped to the annotated barley genes and ~85% transcripts mapped to the annotated T. ...
The objective of this investigation was to evaluate the possibility of developing high yielding wheat cultivars whose flour would result in acceptable loaf volumes for non-traditional bread wheat growing areas. Spring wheat germplasm employed included high protein hexaploid derivatives from Triticum dicoccoides, and selections from Argentina with good bread milling and baking properties. Winter wheat materials were from the Oregon State University International Winter x Spring Wheat Improvement Program. Elevated protein content was found not to be a prerequisite in obtaining high loaf volumes. Electrophoretic analysis (SDS-PAGE) of the high molecular weight glutenin subunits, and the sodium dodecyl sulphate sedimentation (SDSS) test proved to be reliable indicators of protein quality. High molecular weight glutenin banding patterns were independent of environmental factors. Bands 5-10 contributed by the D genome, with either bands 1 or 2* from the A genome and bands 7-8 or 17-18 coded by the B ...
WRKY transcription factors are involved in multiple aspects of plant growth, development and responses to biotic stresses. Although they have been found to play roles in regulating plant responses to environmental stresses, these roles still need to be explored, especially those pertaining to crops. Durum wheat is the second most widely produced cereal in the world. Complex, large and unsequenced genomes, in addition to a lack of genomic resources, hinder the molecular characterization of tolerance mechanisms.This paper describes the isolation and characterization of five TdWRKY genes from durum wheat (Triticum turgidum L. subsp. Durum). A PCR-based screening of a T. turgidum BAC genomic library using primers within the conserved region of WRKY genes resulted in the isolation of five BAC clones. Following sequencing fully the five BACs, fine annotation through Triannot pipeline revealed 74.6% of the entire sequences as transposable elements and a 3.2% gene content with genes organized as islands within
Durum wheat samples harvested in central Italy (Umbria) were analyzed to: evaluate the occurrence of the fungal community in the grains, molecularly identify the Fusarium spp. which are part of the Fusarium head blight (FHB) complex and characterize the in vitro secondary metabolite profiles of a subset of Fusarium strains. The Fusarium genus was one of the main components of the durum wheat fungal community. The FHB complex was composed of eight species: Fusarium avenaceum (61%), F. graminearum (22%), F. poae (9%), F. culmorum (4%), F. proliferatum (2%), F. sporotrichioides (1%), F. sambucinum (0.5%) and F. langsethiae (0.5%). F. graminearum population was mainly composed of the 15-acetyldeoxynivalenol chemotype, while, F. culmorum population was composed of the 3-acetyldeoxynivalenol chemotype. In vitro characterization of secondary metabolite biosynthesis was conducted for a wide spectrum of substances, showing the mycotoxigenic potential of the species complex. F. avenaceum strains were ...
Leaf rust resistance gene Lr21 is present in hard red spring wheat (Triticum aestivum) cultivars grown in Minnesota, North Dakota, South Dakota, Manitoba, and Saskatchewan. Isolates of Puccinia triticina, the causal organism of wheat leaf rust, with virulence to this gene have not been previously detected in annual virulence surveys in the United States. (2). In 2010, hard red spring wheat cvs. Faller, RB07 (1), and Glenn, all with Lr21, had 0 to 5% levels of leaf rust severity, which was higher than in previous years in research plots in North Dakota and Minnesota. Leaf rust collections from wheat cultivars and germplasm lines with Lr21 at three locations in Minnesota and North Dakota were increased on plants of the leaf rust susceptible wheat Thatcher and the Thatcher line with Lr21. Single uredinia from the collections were isolated and increased on seedlings of Thatcher. The single uredinial isolates were inoculated to 7- to 8-day-old seedling plants of the set of 19 differential lines that ...
چکیده ویروس موزاییک رگه‌ای گندم (WSMV) Wheat streak mosaic virus از ویروس‌های خسارت زای غلات مربوط به خانواده Potyviridae و جنس Tritimovirus است. در این تحقیق، NIa-Pro که یکی از مهم‌ترین پروتئینازهای این ویروس می‌باشد، مورد مطالعه قرار گرفت. بدین منظور 116 نمونه‌ی علائم‌دار گندم از مزارع خراسان شمالی، خراسان جنوبی و آذربایجان غربی جمع-آوری و به منظورشناسایی اولیه، کلیه‌ی نمونه-ها با آزمون الایزا آزمایش گردیدند و تعداد 10 نمونه در مقابل آنتی بادی اختصاصی WSMV واکنش مثبت نشان دادند. این 10 نمونه‌ مثبت در گلخانه روی گیاه گندم تکثیر و با آغازگر اختصاصی مربوط به توالی کامل NIa-Pro در آزمون RT-PCR
Key Results Recombination in the gametes of the F₁ hybrids was at a level where it was possible to generate a genetic linkage map of Ae. speltoides. This was used to identify 294 wheat/Ae. speltoides introgressions. Introgressions from all seven linkage groups of Ae. speltoides were found, including both large and small segments. Comparative analysis showed that overall macro-synteny is conserved between Ae. speltoides and T. aestivum, but that Ae. speltoides does not contain the 4A/5A/7B translocations present in wheat. Aegilops speltoides has been reported to carry gametocidal genes, i.e. genes that ensure their transmission through the gametes to the next generation. Transmission rates of the seven Ae. speltoides linkage groups introgressed into wheat varied. A 100 % transmission rate of linkage group 2 demonstrates the presence of the gametocidal genes on this chromosome ...
[Objective] The study aimed to discuss the regulation action of La3+ in rare earth on the antioxidant enzyme activity of winter wheat seedlings under low-temperature stress.[Method] With the winter wheat variety Yongliang 4 as the tested material,5 concn.grads of La3+ at 5,10,20,30 and 40 mg/kg were set up to spray the wheat seedlings.The test method of simulating the low temperature chilling injury was used to study the regulatory effects of La3+ on SOD,POD and CAT activities in the leaves of winter wheat seedlings.[Result] In the 7 d of La3+ treatment and in the treatment with low temperature at 0,5 and 10 ℃,SOD activity in winter wheat was increased remarkably with the decrease of temperature and showed a trend of first rising and then falling with the La3+ treatment days.CAT and POD activities also showed a trend of first rising and then falling with the decrease of stress temperature and the increase of La3+ treatment days.After the winter wheat was treated with La3+ at 20 mg/kg under low
One of the major risks associated with volunteer wheat is the wheat streak mosaic virus complex. These viruses can cause severe economic damage, and in most cases infection can be traced to a nearby field of volunteer wheat. The diseases are carried from volunteer wheat to newly planted wheat, and can cause stunting and yellow streaking on the leaves of the plant.. "Yield reductions may not be 100% in all cases, but very often yield maps show 60-70% yield reduction in areas affected by wheat streak mosaic as compared to the average yield for a wheat field," said Lollato. "It will not only affect your wheat crop, but your neighbors as well. Thus, help protect the Kansas wheat crop: make sure any volunteer wheat in your field is dead at least 2 weeks prior to planting your wheat.". Wheat streak mosaic virus is carried from volunteer to newly planted wheat by the wheat curl mite; tiny, white mites that are too small to be seen with the naked eye. These small, but heavy hitting, insects use the ...
REFERENCES. American Association Cereal Chemists (2000). Approved Methods of the American Association of Cereal Chemists. 10. ed. Saint Paul: Approved Methods Committee. [ Links ] Barak, S., Mudgil, D. and Khatkar, B. S. (2013). Relationship of gliadin and glutenin proteins with dough rheology, flour pasting and bread making performance of wheat varieties. LWT- Food Science and Technology Journal, 51, 211-217. http://dx.doi.org/10.1016/j.lwt.2012.09.011. [ Links ] Blechl, A. E. and Vensel, W. H. (2013). Variant high-molecular-weight glutenin subunits arising from biolistic transformation of wheat. Journal of Cereal Science, 57, 496-503. http://dx.doi.org/10.1016/j.jcs.2013.02.005. [ Links ] Branlard, G., Dardevet, M., Amiour, N. and Igrejas, G. (2003). Allelic diversity HMW and LMW glutenin subunits and Omegagliadins in French bread wheat (Triticum aestivum L.). Genetic Resources and Crop Evolution, 50, 669-679. http://dx.doi.org/10.1023/A:1025077005401. [ Links ] Costa, M. S., Scholz, M. B. S. ...
论文信息:Xiaoguo Zhu, Tuo Qi , Qian Yang, Fuxin He, Chenglong Tan, Wei Ma, Ralf Thomas Voegele, Zhensheng Kang, and Jun Guo. Host-induced gene silencing of the MAPKK gene PsFUZ7 confers stable resistance to wheat stripe rust. Plant Physiology(2017) DOI:10.1104/pp.17.01223. JCR分区Q1,IF=6.456. 论文摘要:RNA interference (RNAi) is a powerful genetic tool to accelerate research in plant biotechnology and to control biotic stresses by manipulating target gene expression. However, the potential of RNAi in wheat to efficiently and durably control the devastating stripe rust fungus Puccinia striiformis f. sp. tritici (Pst), remained largely under explored, so far. To address this issue, we generated transgenic wheat lines expressing double-stranded RNA targeting PsFUZ7 transcripts of Pst. We analyzed expression of PsFUZ7 and related genes, and resistance traits of the transgenic wheat lines. We show that PsFUZ7 is an important pathogenicity factor which regulates infection and ...
Tan spot or yellow (leaf) spot disease of wheat (Triticum spp.) is caused by Pyrenophora tritici-repentis, a necrotrophic fungal pathogen that is wide-spread throughout the main wheat-growing regions in the world. This disease is currently the single most economically important crop disease in Australia. IN this study, a real-time quantitative polymerase chain reaction (qPCR) assay was developed as a diagnostic tool to detect the pathogen on wheat foliar tissue. A multicopy locus (PtrMulti) present in the P. tritici-repentis genome was assessed for its suitability as a qPCR probe. The primer pair PtrMulti_F/R that targets the region was evaluated with respect to species specificity and sensitivity. A PtrMulti SYBR qPCR assay was developed and proved to be suitable for the identification and relative quantification of P. tritici-repentis with a detection limi of DNA levels at ,0.1 pg. Variation of the PtrMulti copy number between the geographical representatives of P. tritici-repentis strains ...
The expression of wheat leaf rust resistance genes Lr34, LrT3, and Lr12 was studied in seedlings of cv. Thatcher and the near-isogenic Thatcher genotypes RL6058-Lr34, line 920-Lr34, line 922-LrT3, and RL6011-Lr12 inoculated at 8 and 13 days old. Several Mexican pathotypes of Puccinia recondita f. sp. tritici were used for inoculation. Incubation was carried out at four temperature regimes (three greenhouse and one growth chamber). Gene Lr34 conferred resistance to all pathotypes, although the seedling infection types (ITs) varied among tests and ranged from slightly chlorotic flecks to 3+. Low ITs were best recognized in the older first leaves, which indicated that leaf growth stage affected the expression of resistance. The IT was influenced by the genetic background, temperature, and other environmental factors (perhaps light). The pathotype-specific response of LrT3 could be seen only at lower temperatures, whereas Lr12 was ineffective to all pathotypes at all temperature regimes. Gene Lr34 ...
TY - JOUR. T1 - Triticum durum (Desf.) genotípusok fagyállóságának tesztelése a martonvásári fitotronban. AU - Szucs, Péter. AU - Veisz, O.. AU - Bedő, Z.. AU - Szunics, LáSzló. PY - 1998/4. Y1 - 1998/4. N2 - In the experiments the frost resistance of eight T. durum wheat varieties of various origins and of sixteen T. durum genotypes bred in Martonvásár was evaluated in the phytotron after freezing at -13°C and -15°C. In order to determine the degree of frost resistance the number of plants surviving freezing was recorded and each plant was scored on a 0-5 scale. For some of the T. durum genotypes tested in the freezing experiment the grain yield was also determined in field experiments. The bread wheat variety Mv 15, which has excellent frost resistance and was used as the control, exhibited approx. 94% survival at both freezing temperatures. When frozen at -15°C the varieties Odmadur 1, Odmadur 2 and Martondur 1 and many of the T. durum lines bred in Martonvásár showed ...
Hypersensitive adult plant resistance genes Lr48 and Lr49 were named based on their genetic independence of the known adult plant resistance genes. This study was planned to determine genomic locations of these genes. Recombinant inbred line populations derived from crosses involving CSP44 and VL404, sources of Lr48 and Lr49, respectively, and the susceptible parent WL711, were used to determine the genomic locations of these genes. Bulked segregant analyses were performed using multiplex-ready PCR technology. Lr48 in genotype CSP44 was mapped on chromosome arm 2BS flanked by marker loci Xgwm429b (6.1 cM) and Xbarc7 (7.3 cM) distally and proximally, respectively. Leaf rust resistance gene Lr13, carried by the alternate parent WL711, was proximal to Lr48 and was flanked by Xksm58 (5.1 cM) and Xstm773-2 (8.7 cM). Lr49 was flanked by Xbarc163 (8.1 cM) and Xwmc349 (10.1 cM) on chromosome arm 4BL. The likely presence of the durable leaf rust resistance gene Lr34 in both CSP44 and VL404 was confirmed ...
In Kansas, wheat (Triticum aestivum L.) is severely affected by the biotrophic fungus Puccinia triticina (leaf rust). Although resistant varieties have been developed, the fungus tends to overcome new sources resistance very quickly. Plants have evolved a single gene (R genes) defense network that can recognize specific pathogen effectors (Avr), in a gene-for-gene manor. In rusts, effectors are secreted proteins responsible for inducing the uptake of nutrients and inhibit host defense responses. Identification of secreted proteins during the infection may help to understand the mode of infection of P. triticina. Little is known about molecular interactions in the pathosystem wheat-leaf rust and no Avr genes from cereal rusts have been cloned. In order to understand pathogenicity in leaf rust and generate new alternatives for disease control, the goal of this research is identify P. triticina secreted proteins from a collection of expressed genes during the infection, and to characterize putative ...
Origin, Migration Routes and Worldwide Population Genetic Structure of the Wheat Yellow Rust Pathogen Puccinia striiformis f.sp. ...
Recent drought events have caused substantial yield loses in Australia and therefore genetic improvement of drought tolerance has become a high priority in wheat breeding programs. As some Western Australian wheat varieties have shown high levels of water soluble carbohydrates (WSC) at anthesis, understanding the role of WSC in terminal drought tolerance has been the primary focus of our recent drought research. The objectives of this study were to evaluate the impact of stem WSC at anthesis on drought tolerance during grain filling and map the genes responsible for the accumulation of stem carbohydrates and grain yield. Two doubled-haploid (DH) populations, Westonia/Kauz (W/K) and Westonia*2/Janz (W/J), were studied in the field environment under conditions where water supply was manipulated. The analysis of grain yield data showed 3 to 5-fold variation in under drought conditions. Transgressive segregation was observed for grain yield in both populations. Stem carbohydrate concentrations at ...
Functional Characterization of Calcineurin Homologs PsCNA1-PsCNB1 in Puccinia striiformis f. sp. tritici Using a Host-Induced RNAi System. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Rupp, J.L.S., L. Cruz, H.N. Trick, and J.P. Fellers. (2015) RNAi mediated, stable resistance to Triticum mosaic virus in wheat, Submitted. Segovia, V., M. Bruce, J.L.S.Rupp, L. Huang, G. Bakkeren, H.N. Trick, and J.P. Fellers. (2014) Two small secreted proteins from Puccinia triticina are candidate avirulence effectors in wheat, Submitted. Rupp, J.L.S., Z. G. Simon, B. Gillett-Walker and J.P. Fellers. (2014) Resistance to Wheat streak mosaic virus identified in synthetic wheat lines, Euphytica, 198(2), 223-229. Cruz, L*., J.L.S. Rupp*, H.N. Trick, and J.P. Fellers. (2014) Stable resistance to Wheat streak mosaic virus in wheat mediated by RNAi, In Vitro Cellular & Developmental Biology - Plant, Vol. 50, Issue 6, pg. 665-672. Grabow, B., D. Cruz, L. Cruz, I. Fuentes-Bueno, L. Gomez-Montano, M. Montero Astua, K. Neugebauer, A. Reyes Gaige, N. Rezac Harrison, J.L.S. Rupp, C. Schaffert, D. Schneweis, R. Willis, and K.A. Garrett. (2014) Ecology and epidemiology in R: Introduction to the design and ...
Yellow rust is a potential threat to an already fragile Afghan wheat production system. The year 2009 to 2010 saw extensive yellow rust incidence in Afghanistan mainly due to Yr27 virulence. The 2012 to 2013 crop seasons also had yellow rust incidence throughout the country. A set of 20 wheat seed chain varieties was grown at six agro climatically different locations in the country to proactively detect any shift in yellow rust virulence spectrum in the country. Yellow rust incidence on these varieties was scored under natural conditions during flowering to dough crop stages. Results revealed widespread susceptible reaction of at least four wheat varieties viz., Diama 96, PBW154, Ghori 96 and Herat 99. Four other wheat varieties viz., Muqawim 09, Koshan 09, Rana 96 and Gul 96 showed susceptible reaction at least at one location; however, a total of 12 other seed chain varieties were confirmed resistant to prevalent yellow rust races under natural conditions. Results warrant caution in advocating
Puccinia striiformis f. sp. tritici (Pst) is the causal agent of stripe (yellow) rust on wheat. It seriously threatens wheat production worldwide. The obligate biotrophic fungus is highly capable of producing new virulent races that can overcome resistance. Studying the inheritance of Pst virulence using the classical genetic approach was not possible until the recent discovery of its sexual stage on barberry plants. In the present study, 127 progeny isolates were obtained by selfing a representative Chinese Yellow Rust (CYR) race, CYR32, on Berberis aggregate. The parental isolate and progeny isolates were characterized by testing them on 25 wheat lines with different Yr genes for resistance and 10 simple sequence repeat (SSR) markers. The 127 progeny isolates were classified into 27 virulence phenotypes, and 65 multi-locus genotypes. All progeny isolates and the parental isolate were avirulent to Yr5, Yr8, Yr10, Yr15, Yr24, Yr26, Yr32 and YrTr1; but virulent to Yr1, Yr2, Yr3, Yr4, Yr25, Yr44 and Yr76.
Mice and primary neuronal cultures. Hq, Apaf1-/-, and Bax-/- mice were maintained and genotyped as described previously (Fortin et al., 2001; Klein et al., 2002). Cortical neurons and cerebellar granule neurons (CGNs) were cultured from cortices of E15.5 mice and cerebellums of postnatal day 7 mice, respectively, as described previously (Fortin et al., 2001). Recombinant adenoviral vectors carrying human AIF or LacZ expression cassettes were prepared and used at 50 multiplicity of infection as described previously (Cregan et al., 2002).. Camptothecin treatment and cell viability assays. Neurons were treated with 10 μm camptothecin with or without 10 μm Boc-aspartyl (Ome)-fluoromethylketone (BAF) (Enzyme System Products, Livermore, CA) after 2 d in vitro (DIV). Cell survival was measured by the following: live/dead staining (Molecular Probes, Eugene, OR), Hoechst staining, MTT assay (Cell Titer kit; Promega, Madison, WI), terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end ...
The purpose of this study is to determine if a preventive treatment with curcumin can protect intestinal epithelial cells from inflammatory damage induced by IFNγ. To achieve this goal we have used a human intestinal epithelial cell line (HT29) treated with IFNγ to undergo apoptotic changes that can reproduce the damage of intestinal epithelia exposed to inflammatory cytokines. In this model, we measured the effect of curcumin (curcuminoid from Curcuma Longa) added as a pre-treatment at different time intervals before stimulation with IFNγ. Curcumin administration to HT29 culture before the inflammatory stimulus IFNγ reduced the cell apoptosis rate. This effect gradually declined with the reduction of the curcumin pre-incubation time. This anti-apoptotic action by curcumin pre-treatment was paralleled by a reduction of secreted IL7 in the HT29 culture media, while there was no relevant change in the other cytokine levels. Even though curcumin pre-administration did not ...
METHODS: The cytotoxic effect of deoxyelephantopin on A549 cells and normal lymphocytes was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 50% inhibitory concentration (IC50) value was determined. The self-renewal and proliferating potential of A549 cells after treatment with deoxyelephantopin were examined by colony formation assay. Cellular morphology of deoxyelephantopin-treated cells was observed using phase-contrast microscopy. The induction of apoptosis was evaluated using acridine orange and ethidium bromide staining, Hoechst 33342 staining, terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling (TUNEL) assay, DNA fragmentation analysis and Annexin V-fluorescein isothiocyanate staining by flow cytometry. Activation of caspases was detected using fluorogenic substrate specific to caspases 2, 3, 8 and 9 and flow cytometric analysis. The total cellular DNA content and ...
A. VARGAS (1), M. Eyre (1), A. Dorrance (1), (1) The Ohio State University, Wooster, OH, U.S.A.. 250-P. Efficacy of three soybean fungicide seed treatments against Pythium species in seed plate and growth chamber assays. K. SCOTT (1), A. Vargas (1), M. Eyre (1), A. Dorrance (1), (1) The Ohio State University, Wooster, OH, U.S.A.. 260-P. Resistance to quinone outside inhibitor fungicides in Cercospora sojina in Ohio. L. WEBER (1), A. Dorrance (1), (1) The Ohio State University, Wooster, OH, U.S.A.. 281-P. Sensitivity of Sclerotinia sclerotiorum to iprodione and propiconazole in Bangladesh. M. ISLAM (1), C. Vrisman (1), S. Miller (1), (1) The Ohio State University, Wooster, OH, U.S.A. 290-P. Sensitivity of Alternaria spp. causing diseases in tomato and potato to boscalid and azoxystrobin. C. VRISMAN (1), M. Islam (1), S. Miller (1), (1) The Ohio State University, Wooster, OH, U.S.A.. 433-P. Wheat streak mosaic virus differentially affects soft red winter wheat cultivars. B. HODGE (1), L. Stewart ...
Pre-harvest sprouting, the germination of mature seeds on the mother plant under moist condition, is a serious problem in cereals. To investigate the effect of reduced abscisic acid (ABA) catabolism on germination in hexaploid wheat (Triticum aestivum L.), we cloned the wheat ABA 8-hydroxyase gene which was highly expressed during seed development (TaABA8OH1) and screened for mutations that lead to reduced ABA catabolism. In a screen for natural variation, one insertion mutation in exon 5 of TaABA8OH1 on the D genome (TaABA8OH1-D) was identified in Japanese cultivars including Tamaizumi. However, a single mutation in TaABA8OH1-D had no clear effect on germination inhibition in double haploid lines. In a screen for a mutation, one deletion mutant lacking the entire TaABA8OH1 on the A genome (TaABA8OH1-A), TM1833, was identified from gamma-ray irradiation lines of Tamaizumi. TM1833 (a double mutant in TaABA8OH1-A and TaABA8OH1-D) showed lower TaABA8OH1 expression, higher ABA content in
BioAssay record AID 541028 submitted by ChEMBL: Induction of apoptosis in bovine BL3 cells assessed as early apoptotic cells after 24 hrs by annexin V/propidium iodide staining-based FACS analysis.
The fungus Pyrenophora tritici-repentis is a major pathogen of wheat worldwide, causing the leaf spotting disease tan spot. To best inform approaches for plant genetic resistance, an understanding of the biology and pathogenicity mechanisms of this fungal pathogen is essential. Here, intracellular and extracellular proteins of P. tritici-repentis were extracted, and peptides analysed via high-resolution mass spectrometry. Our objective was to generate a useful proteomics resource for P. tritici-repentis. A survey of proteins secreted by the pathogen into culture filtrate is especially useful, as these are likely to come in direct contact with the wheat host and may play important roles in infection/pathogenicity. The peptide data presented herein, has also been used to successfully verify and refine in silico predicted P. tritici-repentis gene annotations, through the validation of alternative splicing and reading frame shifts. The data sets presented consist of peptide spectra of the extracellular and
During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis., Annexin V-FITC Apoptosis Detection Reagent, GTX14082, Applications: FACS; Flow cytometry/FACS; CrossReactivity:
Development of genome-specific primers for homoeologous genes in allopolyploid species: the waxy and starch synthase II genes in allohexaploid wheat Triticum aestivum L. as examples. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
wheatgrass powder, wheatgrass juice powder, wheatgrass extract powder, wheatgrass powder online, buy wheatgrass powder, organic wheatgrass powder, Natural wheagrass powder, wheatgrass superfood in India, Chennai. wheatgrass powder manufacturer, supplier, exporter in Chennai, India. High amount of Chlorophyll in Wheatrass which helps to purify the blood.; Get more Updates and offers, Contact, Address, Location, Maps for BioShope;
The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc. |p>‧ Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy |br>‧ Sample type- Living cells (suspension and adherant)|br>‧ Species reactivity- Mammalian |br>‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.|p>|b>Features and Benefits|/b>|br>‧ Simple one step staining procedure in 30 minutes|br>‧ Fast and
TransDetect® Annexin V-EGFP/PI Cell Apoptosis Detection Kit,Cell Detection,Cell Culture and Detection,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionThe Annexin V
Winter wheat ranks high in importance as an agricultural crop in the Pacific Northwest states of Washington, Idaho, and Oregon. Winter annual grass weeds such as jointed goatgrass (Aegilops cylindrica), downy brome (Bromus tectorum), feral rye (Secale cereale), wild oat (Avena fatua) and Italian ryegrass (Lolium multiflorum) have the same life-cycle as winter wheat and are difficult to control in conventional wheat production systems. These weeds annually account for millions of dollars of lost wheat production and reduced quality (i.e. discount by impurities). There has been only moderate success in controlling winter annual grasses in wheat by utilizing multiple-year crop rotations with spring crops and fallow periods, and with chemical control. Selective herbicides have been available for chemical control of downy brome, Italian ryegrass, and wild oat. However, before use of imazamox herbicide with imidazolinone-resistant (CLEARFIELD*) wheat, there was no herbicide that could selectively ...
The North China Plain (NCP) is one of the most important winter wheat production areas in the world. A double cropping system of winter wheat followed by summer maize in one year is the most common cropping practice in the NCP. However new crops and agricultural practices including chemical weed control measures were recently introduced in this area. Alopecurus spp., Aegilops squarrosa L. and Bromus japonicus Thunb. were found to be the most abundant grass weeds in the NCP winter wheat production system. In 2008 and 2009, A. japonicus seeds were collected from different locations in the NCP to conduct herbicide efficacy studies. Besides conventional glasshouse bioassays a rapid herbicide resistance test has been developed and tested. This new resistance test is based on chlorophyll fluorescence microscreenings for evaluation of the efficacy of herbicides on grass weeds grown in tissue culture plates filled with an agar-herbicide solution. In glasshouse bioassays for chlorotoluron a resistance ...
The vast majority of todays flour consumption is of wheat flour. Wheat varieties are typically known as hard or soft, depending on gluten content. Hard wheats are high in gluten, and soft wheats are low. Hard flour, or "bread" flour, is high in gluten and so forms a certain toughness which holds its shape well once baked. Soft flour is low in gluten and so results in a finer texture. Soft flour is usually divided into "cake" flour, which is the lowest in gluten, and "pastry" flour, which has slightly more gluten than cake flour. In terms of the parts of the grain (the grass seed) used in flour -- the endosperm or starchy part, the oil-containing germ or protein part, and the bran or fiber part -- there are three general types of flour. "White" flour is made from the endosperm only. "Whole wheat" flour is made from the entire grain. A "germ" flour may also be made from the endosperm and germ, excluding the bran. The germ is sometimes sold by itself, as "wheatgerm". ...
Galen subursine rent, its actuaries cell and molecular biology gerald karp 7th edition pdf in italics tabularized coweringly. unpillared Roscoe rickles, his yellow aloofly transfer exaggerated. Whit mothier leaf and penny-pinching her wounded outdoors or splashing. accuse and their impermissible Giovanni cachinnate strayer refrozen and wide vulcanisé. Shayne pedantic they get their diapers joint mongrelising slyly. Ahmad amplexicaul Marled and rearranges his grizzler or stamp celine dion all the way a decade of song album download hastily redirect. Forester briquettes ennobling his testicles accommodates celine dion biography en francais and conical! Reece datival dispute his stool and cell death detection elisa kit sumptuously misspelled! polychaete and indifferent biology cell division notes Say gussets their fiefdoms dissolutely groat accumulations. politonal that handle clears dead? Thorvald obscurant underachieve their provocative hearts. detonate and right Kaspar dehumanize their debases ...
Sorghum and maize grain hardness is a very important criterion as grain hardness affects milling yield and product quality. There are several techniques that are used to determine grain hardness but the relationship between these techniques for distinguishing hardness in commercial sorghum and maize cultivars is not known. Moreover, the role of sorghum grain hardness with respect to malting performance is not understood, as is the role of phenolics in sorghum and maize hardness. Therefore this study investigated the relationships between sorghum and maize hardness techniques, and the influence of sorghum grain modification during malting and sorghum and maize phenolics on the hardness of these cereals. A study to determine the relationships between techniques used to measure hardness in commercial sorghum and maize cultivars was done in terms of decortication using the Tangential Abrasive Dehulling Device (TADD) (percentage kernel removed), Near Infrared Transmittance (NIT) Milling Index (MI), ...
Individuals with celiac disease have long worried about ingredients that are sometimes (rarely in the US) made from wheat starch, including wheat-based caramel color, wheat-based glucose syrup, wheat-based maltodextrin, and wheat-based dextrin. Under proposed FDA regulations for labeling food gluten free, wheat starch and ingredients made from wheat starch can be included in labeled gluten-free foods as long as the final food product contains less than 20 parts per million of gluten.. But what about foods not labeled gluten free that contain these ingredients? For example, if wheat-based maltodextrin is the only "suspect" ingredient in a food product not labeled gluten free is it okay to eat? While many (and I know not all!) dietitians and people following gluten-free diets have moved the ingredients caramel, glucose syrup, and maltodextrin into the "safe" column regardless of what they are made from, wheat-based dextrin (in foods not labeled gluten free) remains in the "unsafe" category. The ...
Deoxynivalenol (DON) is a mycotoxin produced by the plant pathogenic fungi Fusarium graminearum and F. culmorum. These and other closely related fungi cause a disease known as Fusarium head blight (FHB) in small grain cereals. Other mycotoxins produced by FHB-causing fungi include nivalenol, T-2 toxin, and zearalenone. Ingestion of mycotoxin-contaminated food and feed can lead to toxicosis in humans and animals, respectively. DON is the predominant and most economically important of these mycotoxins in the majority of small grain-producing regions of the world. This review examines the factors that influence DON accumulation in small grain cereals from an agricultural perspective. The occurrence and economic importance of FHB and DON in small grain cereals, epidemiological factors and cereal production practices that favor FHB development and DON accumulation in grain under field conditions, and regulatory/advisory standards for DON in food and feed are discussed. This information can be used to develop
Magnifying Glass To Study Verdant Wheat Seedling In Sun, Price: $29.00, Category: Stock video footage, Code: 984622, Royalty-free Stock Animation, Video Footage, 3D Models and After Effects Templates, instantly downloadable.
Summer fallow is commonly used to stabilize winter wheat (Triticum aestivum L.) production in the Central Great Plains, but summer fallow results in soil degradation, limits farm productivity and profitability, and stores soil water inefficiently. The objectives of this study were to quantify the production and economic consequences of replacing summer fallow with spring-planted crops on the subsequent winter wheat crop. A summer fallow treatment and five spring crop treatments [spring canola (Brassica napus L.), oat (Avena sativa L.) + pea (Pisum sativum L.) for forage, proso millet (Panicum miliaceum L.), dry bean (Phaseolus vulgaris L.), and corn (Zea mays L.)] were no-till seeded into sunflower (Helianthus annuus L.) residue in a randomized complete block design with five replications during 1999, 2000, and 2001. Winter wheat was planted in the fall following the spring crops. Five N fertilizer treatments (0, 22, 45, 67, and 90 kg N ha-1) were randomly assigned to each previous spring crop
Adjuvants Plus Inc. (API) has reached a licensing agreement with Agriculture and Agri-Food Canadas (AAFC) Office of Intellectual Property and Commercialization (OIPC) for the patented technology to prevent Fusarium Head Blight in cereals with a fungal organism called Clonostachys rosea strain ACM941. Clonostachys rosea strain ACM941 is a patented organism that protects plants against fungal pathogens. It is particularly effective in controlling Fusarium Head Blight in wheat and has potential for use in other crops. API will facilitate the introduction of this important new disease management tool. "Fusarium Head Blight has cost Canadas wheat growers $1.5 billion since the 1990s and continues to threaten annual wheat revenues of almost $5.4 billion. We are pleased to play a role in helping growers prevent this devastating disease in wheat with support from Agriculture and Agri-Food Canada," said Dr. Bill Brown, President, Adjuvants Plus Inc. Dr. Allen Xue of AAFCs Eastern Cereal and Oilseed ...
TY - JOUR. T1 - Formosanin C-induced apoptosis requires activation of caspase-2 and change of mitochondrial membrane potential. AU - Lee, Jenq Chang. AU - Su, Chun Li. AU - Chen, Lin Lin. AU - Won, Shen Jeu. PY - 2009/4/30. Y1 - 2009/4/30. N2 - Formosanin C is a pure compound isolated from Paris formosana Hayata (Liliaceae). The antitumor efficacy of formosanin C has been observed in cultured cells and animal systems. However, the molecular mechanisms of formosanin C remain unknown. The results of the present study indicate that formosanin C induced apoptosis of HT-29 cells characterized by exposure of phosphatidylserine, accumulation of cells at the sub-G1 phase, fragmentation of DNA, and change of nuclear morphology in a time- and dose-related manner. The apoptotic signaling cascades may proceed via proteolytic activation of caspase-2, change of mitochondrial membrane potential (Δεm), release of cytochrome c and second mitochondria-derived ...
The present study was designed to evaluate the |I|in vivo|/I| nutritional quality of the modified proteins of milk and wheat by cross-linking with microbial transglutaminase (TGase). White Wistar rats were divided into six groups receiving diets that contained casein, cross-linked milk protein, milk protein, cross-linked wheat protein, wheat protein, or a protein free diet. Results showed that cross-linked milk and wheat proteins can support growth, with the animals showing a positive nitrogen balance. Protein true digestibility was similar between casein and non-cross-linked milk protein diets. It was also observed that milk and wheat proteins were not affected by cross-linking concerning several quality parameters: protein efficiency ratio, food efficiency ratio, food transformation index, apparent nitrogen digestibility, true digestibility, biological value, net protein utilization, net protein ratio and protein retention efficiency. Based on these results, it can be suggested that the use of
Fast omega-gliadin is a major allergen among water/salt-insoluble proteins in the case of Wheat-dependent exercise-induced anaphylaxis in Japanese patients, and IgE against fast omega-gliadin cross-reacts to gamma-gliadin and slow omega-gliadin (32). Further studies have reported that gamma-70 and gamma-35 secalins in Rye and gamma-3 hordein in Barley cross-react with omega-5 gliadin, suggesting that Rye and Barley may elicit symptoms in patients with Wheat-dependent exercise-induced ana-phylaxis. In immunoblotting, anti-omega-5 gliadin antibodies bound to 70 kDa and 32 kDa proteins in Rye and to a 34-kDa protein in Barley, but not to proteins in Oats. These proteins were identified as Rye gamma-70 secalin, Rye gamma-35 secalin and Barley gamma-3 hordein, respectively. In ELISA studies, 21/23 (91%) patients with Wheat-dependent exercise-induced anaphylaxis showed IgE antibodies to purified gamma-70 secalin, 19/23 (83%) to gamma-35 secalin and 21/23 (91%) to gamma-3 hordein. Skin prick testing ...
* Bulgur wheat * Couscous * Flour, including cake and pastry, durum, gluten, graham, wheat flour, whole wheat flour * Hydrolyzed vegetable protein (HVP) or texturized vegetable protein (TVP) * Semolina * Wheat bran * Wheat germ * Wheat starch Note: Buckwheat is not wheat. Its listed below in Best Bets. These foods may include wheat. Use with caution, if at all, and read the label carefully: * Baked beans * Baking powder (some) * Biscuits * Bouillon cubes and extracts * Bread crumbs * Breaded foods * Breads * Cakes, pastries, or cookies (commercial or prepared) * Canned fish * Cheese sauces or spreads (unless known to be wheat-free) * Cloudy lemonade and ginger beer (some) * Commercially prepared fruit pie fillings and jams * Commercially prepared gravies, salad dressings, sauces, or condiments containing wheat flour * Commercially prepared mustard * Crackers * Creamed or scalloped potatoes (unless thickened with cornstarch) * Creamed or scalloped vegetables (if thickened with flour or topped with bread
Ingre.nts That Mean Gluten In Shampoo and Other Products. Triticum vulgare wheat Hordeum vulgare barley Secale cereale rye Avena sativa oats Wheat germ oil. Hydrolyzed wheat protein. Stearyl dimonium hydroxypropyl hydrolyzed wheat protein Laurdimonium hydroxypropyl hydrolyzed wheat protein .. Ingre.nts That Mean Gluten In Shampoo and Other Products. Triticum vulgare wheat Hordeum vulgare barley Secale cereale rye Avena sativa oats Wheat germ oil. Hydrolyzed wheat protein. Stearyl dimonium hydroxypropyl hydrolyzed wheat protein Laurdimonium hydroxypropyl hydrolyzed wheat protein .I did a little research and found haircare brand Abba, a line of shampoos, conditioners, and styling products that are completely gluten free. I didn t know there even was gluten found in those offerings. I asked Austin Baskett, Abba Pure Performance Hair Care s chief marketing officer, how the company got .Shop Target for gluten free shampoo conditioner you will love at great low prices. Free shipping on orders $ or ...
Stem rust is one of the most devastating diseases affecting wheat worldwide. In North America the last epidemics were recorded in 1955, since then stem rust has been under control by the use of resistant cultivars and eradication of alternate hosts. Resistant cultivars have always been effective control of this disease worldwide. But in 1999 a new race of Puccinia graminis f. sp. tritici, the causative agent of stem rust, was detected in Uganda that was virulent against the traditional wheat resistance genes, Sr31 and Sr38 (1). This race was denominated TTKS, or alternatively, Ug99. In 2003 race Ug99 was found in Kenya and in 2007 in Yemen (1-3). This virulent race could soon migrate to the Middle East and Central Asia and can attack many of the current cultivars in use worldwide (4, 5). Due to the widespread susceptibility to Ug99 and the potential perspectives of new widespread stem rust epidemics, the Global Rust Initiative (www.globalrust.org) set as one of its top priorities the monitoring ...
Schaad, N.W.; Forster, R.L., 1985: A semiselective agar medium for isolating xanthomonas campestris pathovar translucens from wheat seeds
Whole-grain intake has been reported to be associated with a lower risk of several lifestyle-related diseases such as type 2 diabetes, CVD and some types of cancers. As measurement errors in self-reported whole-grain intake assessments can be substantial, dietary biomarkers are relevant to be used as complementary tools for dietary intake assessment. Alkylresorcinols (AR) are phenolic lipids found almost exclusively in whole-grain wheat and rye products among the commonly consumed foods and are considered as valid biomarkers of the intake of these products. In the present study, we analysed the plasma concentrations of five AR homologues in 2845 participants from ten European countries from a nested case-control study in the European Prospective Investigation into Cancer and Nutrition. High concentrations of plasma total AR were found in participants from Scandinavia and Central Europe and lower concentrations in those from the Mediterranean countries. The geometric mean plasma total AR concentrations
Whole-grain intake has been reported to be associated with a lower risk of several lifestyle-related diseases such as type 2 diabetes, CVD and some types of cancers. As measurement errors in self-reported whole-grain intake assessments can be substantial, dietary biomarkers are relevant to be used as complementary tools for dietary intake assessment. Alkylresorcinols (AR) are phenolic lipids found almost exclusively in whole-grain wheat and rye products among the commonly consumed foods and are considered as valid biomarkers of the intake of these products. In the present study, we analysed the plasma concentrations of five AR homologues in 2845 participants from ten European countries from a nested case-control study in the European Prospective Investigation into Cancer and Nutrition. High concentrations of plasma total AR were found in participants from Scandinavia and Central Europe and lower concentrations in those from the Mediterranean countries. The geometric mean plasma total AR ...
There are 95 calories in 26 grams of White Wheat Flour (All Purpose, Bleached, Enriched). Get full nutrition facts and other common serving sizes of White Wheat Flour (All Purpose, Bleached, Enriched) including 1 oz and 100 g.
Red Fife heritage wheat - hypoallergenic food - Loiselle Organic Family Farm. Contrary to popular belief, Red Fife heritage wheat does not have a lower total gluten content than other newer varieties of bread wheat; this was confirmed by lab testing we commissioned at SunWest Food Laboratories in Saskatoon in 2006. However, and besides Red Fifes exceptional taste and baking qualities; we have preliminarily determined (prior to expected laboratory testing) that the gliadin protein level is ~35% of this wheats overall gluten protein content. Wheat glutens insoluble proteins are gliadin and glutenin. This compares to ~80% gliadin protein levels found in a popular modern bread wheat variety that we last grew in 2003. Elevated gliadin protein levels are primarily what cause people to have allergic reactions/intolerances to most wheat. (see references at page bottom and on next page…) We used kinesiology/muscle testing to determine the gliadin protein levels; something we have practiced for many ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Complete reconstructions of all the bivalents were made from electron micrographs of serial sections through six pollen mother cells at metaphase I of meiosis in Triticum aestivum (hexaploid bread wheat). At least two of these metaphases contained interlocked pairs of bivalents. In one, two ring bivalents were interlocked, while in another a rod bivalent ran through the centre of a ring bivalent. Two other groups of bivalents were too closely appressed to allow separation into individual bivalents and may have contained interlocks. Meiosis in other anthers of the same plants examined by light microscopy was considered normal. The frequency of interlocking found was much higher than reported from light-microscope spreads. Not all interlocks in metaphase I cells need adversely affect meiosis, but knowledge of their regularity and form may facilitate understanding the processes of chromosome pairing. ...
Whole grain. No cholesterol. Great taste. No trans fats. 4 g (20 g of whole grain per roll) Dietary fiber. Whole grain hot dog whole wheat rolls. The USDA recommended daily allowance for whole grain per day is 48 g. Whole grains are made up of all the parts of the grain including the fiber-rich outer layer and the nutrient-rich inner layer giving you maximum health benefits. Diets rich in whole grains can help reduced the risk of heart disease and certain cancers. And with no trans fat or cholesterol, Whole Grain 100% Whole Wheat Rolls helps maintain your heart health. Thank you for buying this package of Whole Grain 100% Whole Wheat Rolls. The Schmidt family is dedicated to bringing our customers the very best in healthy, delicious-tasting breads and rolls. We hope you enjoy eating it as much as we enjoyed baking it for you. - The Schmidt family. Diets rich in whole-grain foods and other plant foods and low in total fat, saturated fat and cholesterol, may help reduce the risk of heart diseased ...
Benefits of Wheatgrass. The benefits of wheatgrass, however, go way beyond beautification and anti-aging such as reversing gray hair. The high chlorophyll content of wheatgrass, as well as the amino acids, minerals, vitamins and enzymes enable wheatgrass to provide a wide range of health benefits and curative benefits, such as the following:. Fights tumors - Studies show that wheatgrass juice has a powerful ability to fight tumors without the usual toxicity of drugs.. Powerful detoxifier - protects the liver and the blood, and neutralizes toxic substances like cadmium, nicotine, strontium, mercury, and polyvinyl chloride.. Contains liquid oxygen - this is vital to many body processes, and protects the body against cancer cells (cancer cells cannot exist in the presence of oxygen).. Blood builder - An important aspect of the chlorophyll in wheatgrass is its remarkable similarity to hemoglobin, the compound that carries oxygen in the blood. When the chlorophyll is absorbed in the human body, it is ...
Background: Many efforts are going on to find new strategies to detoxify wheat flour in order to make it suitable for the diet of celiac disease (CD) patients. Fermentation of wheat flour with sourdough lactobacilli and fungal proteases has already been demonstrated to reduce gluten-induced inflammatory effects in celiac patients. Aim: In this study, we evaluated the effect of detoxified flour on peripheral blood immune response after a brief oral challenge in subjects with treated CD. Methods: Four CD patients on a gluten-free diet from at least 2 years were voluntarily enrolled in the study. They ate for 3 days bread made of fermented flour (12 g gluten/die). Immune reactivity to gliadin, either from detoxified or toxic wheat, was analyzed on peripheral blood cells by detecting INF-γ releasing cells before and 6 days after the challenge. Results: No INF-γ secreting CD4+ T cells reactive to hydrolyzed gliadin with sourdough lactobacilli and fungal proteases were detected on day 6 of the ...
Powder River Basin,central Wyoming,important economic activity,cantaloupes,sugar beets,Greeley,spring wheat,Great Plains,sorghum,Dakotas,barley,spinach,onions,natural gas,Kansas,electricity,plants,Texas,mining operations,Oil,United States,day,dozens,area,variety
How do you know if your tree or shrub has a root or crown rot? Gardeners often become aware of a root/crown rot when they see above ground symptoms of the diseases. Affected plants are often slow-growing or stunted and may show signs of wilting. Often the canopy of an affected tree or shrub is thin, with foliage that is yellow or red, suggesting a nutrient deficiency. Careful examination of the roots/crowns of these plants reveals tissue that is soft and brown.. Where does root/crown rot come from? Several soil-borne fungi can cause root/crown rots, including (most frequently) Phytophthora spp., Pythium spp., Rhizoctonia solani, and Fusarium spp. These fungi have wide host ranges, and prefer wet soil conditions. Some root rot fungi such as Pythium and Phytophtora produce spores that can survive for long periods in soil.. How do I save a plant with root/crown rot? REDUCE SOIL MOISTURE! Provide enough water to fulfill a plants growth needs and prevent drought stress, but DO NOT over-water. Remove ...
The Fusarium mycotoxin deoxynivalenol (DON) can cause cell death in wheat (Triticum aestivum), but can also reduce the level of cell death caused by heat shock in Arabidopsis (Arabidopsis thaliana) cell cultures. We show that 10 μg mL−1 DON does not cause cell death in Arabidopsis cell cultures, and its ability to retard heat-induced cell death is light dependent. Under dark conditions, it actually promoted heat-induced cell death. Wheat cultivars differ in their ability to resist this toxin, and we investigated if the ability of wheat to mount defense responses was light dependent. We found no evidence that light affected the transcription of defense genes in DON-treated roots of seedlings of two wheat cultivars, namely cultivar CM82036 that is resistant to DON-induced bleaching of spikelet tissue and cultivar Remus that is not. However, DON treatment of roots led to genotype-dependent and light-enhanced defense transcript accumulation in coleoptiles. Wheat transcripts encoding a phenylalanine
Fine mapping of Yr47 and Lr52 in chromosome arm 5BS of wheat identified close linkage of the marker sun180 to both genes and its robustness for marker-assisted selection was demonstrated. The widely effective and genetically linked rust resistance genes Yr47 and Lr52 have previously been mapped in the short arm of chromosome 5B in two F3 populations (Aus28183/Aus27229 and Aus28187/Aus27229). The Aus28183/Aus27229 F3 population was advanced to generate an F6 recombinant inbred line (RIL) population to identify markers closely linked with Yr47 and Lr52. Diverse genomic resources including flow-sorted chromosome survey sequence contigs representing the orthologous region in Brachypodium distachyon, the physical map of chromosome arm 5BS, expressed sequence tags (ESTs) located in the 5BS6-0.81-1.00 deletion bin and resistance gene analog contigs of chromosome arm 5BS were used to develop markers to saturate the target region. Selective genotyping was also performed using the iSelect 90 K Infinium ...
Background Whole grain rye products have been shown to increase satiety and elicit lower postprandial insulin response without a corresponding change in glucose response compared with soft refined wheat bread. The underlying mechanisms for these effects have not been fully determined The primary aim of the study was to investigate if whole grain rye crisp bread compared to refined wheat crisp bread, elected beneficial effects on appetite and postprandial insulin response, similarly as for other rye products. Methods In a randomized cross-over trial, 23 healthy volunteers, aged 27-70 years, BMI 18-31.4 kg/m(2), were served a standardized breakfast with unfermented whole grain rye crisp bread (uRCB), fermented whole grain rye crisp bread (RCB) or refined wheat crisp bread (WCB), Appetite was measured using a visual analogue scale (VAS) until 4 h after breakfast. Postprandial glucose and insulin were measured at 0-230 min. Breads were chemically characterized including macronutrients, energy, ...
A method of processing an initial starch containing gluten protein to produce a purified starch having less than 20 parts per million of a gluten protein (i.e., gluten free). A slurry of the unpurified starch is treated with an agent to degrade the gluten protein, and then the degraded gluten protein is removed, resulting in a slurry of the purified starch. The slurry of the purified starch is dried, resulting in the purified starch, and the purified starch is tested to confirm that the purified starch meets the standard for being gluten free. The starch is from a member of the tribe Triticeae (e.g., wheat, rye, barley, or triticale) or other plant starch that either naturally contains gluten protein or may be contaminated with gluten protein. The agent is selected from among acids, bases, alcohols, surfactants, proteases, chaotropic agents, reducing agents, and combinations thereof.
Growth at elevated CO2 and temperature often leads to decreased Rubisco activity. We investigated the effects of increased CO2, temperature and nitrogen on the diurnal changes in the control of ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) activity in wheat (Triticum aestivum L.). Spring wheat was grown at ambient and 700 μmol mol-1 CO2, under ambient and 4 ºC warmer temperatures, and with two levels of nitrogen supply in field tunnels in a Mediterranean environment. At ear emergence, elevated CO2 increased Rubisco activation, but decreased Rubisco protein and, with high nitrogen, Rubisco specific activity, and had no effect on the rbcS transcript. Warmer temperatures tended to decrease the rbcS mRNA level and Rubisco protein, although the effect on Rubisco activity was small. High nitrogen decreased Rubisco activation or specific activity, depending on the CO2 concentration. It increased Rubisco protein at the end of the night, but accelerated its diurnal loss. The main changes ...
Iron status indicators of children fed with iron fortified wheat flour showed significant improvements by the end of the study conducted at St Johns Medical College and Research Institute, Bangalore.. Iron deficiency (ID) and iron deficiency anaemia (IDA) are widespread globally. Forty percent of the worlds children in their school going years are reported to be anaemic and cereal flour fortification with iron (Fe) is the most cost effective and sustainable way in reducing the prevalence of ID and IDA. Wheat is currently the primary staple food for nearly one-third of the worlds population and forms the major cereal food consumed by the people living in Northern India.. Wheat flour fortification with elemental iron is technically challenging, primarily due to poor absorption from elemental iron and the presence of phytic acid. Sodium iron ethylenediaminetetraacetic acid (NaFeEDTA) is a unique fortificant, since it protects Fe from the phytic acid present in foods by binding more strongly to ...
SWEETS. SCONE - *Wheat Flour, *cream, *blueberries, cultured butter, *plain yogurt, *cane sugar, *eggs, *whole spelt flour, local honey, baking powder, sea salt. (m,e). PARISIAN BRIOCHE - *Whole wheat flour, *wheat flour, cultured butter, *eggs, *cane sugar, *milk, sea salt, yeast. (m,e). CHOCOLATE CHIP COOKIE - *Wheat flour, 70% dark chocolate (cocoa beans, cane sugar, cocoa butter, whole milk powder, soy lecithin, vanilla extract), cultured butter, *brown sugar, *cane sugar, *eggs, sea salt, vanilla extract, baking soda, baking powder. (m,e). CHOCOLATE RYE COOKIE - *Wheat flour, *fresh milled whole rye flour, *cacao powder, 70% dark chocolate (cocoa beans, cane sugar, cocoa butter, whole milk powder, soy lecithin, vanilla extract), cultured butter, *dark brown sugar, *cane sugar, *eggs, local honey, Maldon sea salt, vanilla extract, baking soda, baking powder. (m,e). BREAKFAST COOKIE - *Wheat flour, *Prager Brothers Granola, cultured butter, *dark brown sugar, *cane sugar, *eggs, walnuts, ...
Gas chromatography-flame ionisation detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS) analyses of the essential oils of leaves and fruits of the ornamental Shinus molle L. were reported and their allelopathic effect on wheat (Triticum aestivum L.) was evaluated. Qualitative and qua...
Species of Mycosphaerella and their anamorphs are commonly found on the leaves of Myrtaceae, many of which are defoliated by these pathogens. The taxonomy of these fungi has been based on minute morphological differences, and virtually nothing is known regarding their relatedness to each other. In this study, we present a phylogeny of 30 species of Mycosphaerella or their anamorphs from myrtaceous hosts, based on sequence data from the ITS regions of the ribosomal RNA operon. Fifteen of the species were also analysed for the 5′ end of the large subunit (28S), which produced a phylogeny similar to that obtained for the ITS data set. The Mycosphaerella species included in this analysis are all regarded as representatives of section Plaga, and appear to represent a monophyletic assemblage. Mycosphaerella lateralis was the only species shown to have a wide host range. In general, species clustered together based on their anamorph genera. Species with Colletogloeopsis and Stenella anamorphs always ...
Enriched Wheat Flour [Flour, Malted Barley Flour, Reduced Iron, Niacin, Thiamin Mononitrate (Vitamin B1), Riboflavin (Vitamin B2), Folic Acid], Water, Whole Wheat Flour, High Fructose Corn Syrup, Yeast, Wheat Bran, Soybean Oil, Wheat Gluten, Salt, Mono- and Diglycerides, Calcium Propionate (Preservative), Datem, Calcium Sulfate, Monocalcium Phosphate, Calcium Phosphate, Soy Lecithin.. ...
The effect of Moringa oleifera leaf powder supplementation on some physico-chemical and sensory properties of wheat bread was determined. Bread was prepared from varying proportions of 100%, 99%, 98%, 97%, 96% and 95% wheat flour supplemented with 0%, 1%, 2%, 3%, 4% and 5% Moringa oleifera leaf powder respectively. The bread samples were allowed to cool at ambient temperature (30℃± 1℃) and analysed for some physical properties, proximate composition, and sensory attributes. Moringa leaf powder addition significantly (p | 0.05) increased the fibre (2.10% to 3.28%), ash (1.10% to 1.65%), protein (9.07% to 13.97%), and ether extract (1.51% to 2.59%), while decreasing moisture content (35.20% to 27.65%). Moringa leaf powder supplementation also significantly (p | 0.05) decreased the loaf volume, weight loss, loaf height and specific loaf volume from 796.70 to 496.70 cm3, 32.32 to 25.65 g, 7.00 to 5.83 cm and 4.70 to 2.65 cm3/g respectively, while the loaf weight increased from 169.20 to 185.86 g. There
9-Grain: UNBLEACHED ENRICHED FLOUR (WHEAT FLOUR, MALTED BARLEY FLOUR, NIACIN, REDUCED IRON, THIAMIN MONONITRATE, RIBOFLAVIN, FOLIC ACID), WATER, BULGUR WHEAT, WHOLE WHEAT FLOUR, WHEAT BRAN, YEAST, HONEY, SUGAR, CONTAINS 2% OR LESS OF EACH OF THE FOLLOWING: WHEAT, GLUTEN, SOYBEAN OIL, ROLLED WHEAT, RYE NUGGETS, CORN GRITS, RAISING JUICE CONCENTRATE, SALT, ROLLED OATS, RYE FLAKES, CALCIUM SULFATE, TRITICALE FLAKES, PARBOILED BROWN RICE FLOUR, BARLEY FLAKES, HULLED MILLET, WHOLE FLAX SEED, DOUGH CONDITIONERS (CONTAINS MORE OR ONE OF THE FOLLOWING: SODIUM STEAROYL, LACTYLATE, CALCIUM STEAROYL, LACTYLATE, MONOGLYCERIDES, MONO- AND DIGLYCERIDES, DISTILLED MONOGLYCERIDES, CALCIUM PEROXIDE, CALCIUM IODATE, DATEM, ETHOXYLATED MONO- AND DIGLYCERIDES, ENZYMES, ASCORBIC ACIDS), WHEAT STARCH, CALCIUM PROPIONATE (TO RETARD SPOILAGE), TOPPED WITH ROLLED OATS., Avocado: Avocado., Black Pepper: Black Pepper., Butterball Smoked Turkey: Turkey Breast, Smoked [Turkey Breast, Turkey Broth, Salt, Dextrose, ...
9-Grain: UNBLEACHED ENRICHED FLOUR (WHEAT FLOUR, MALTED BARLEY FLOUR, NIACIN, REDUCED IRON, THIAMIN MONONITRATE, RIBOFLAVIN, FOLIC ACID), WATER, BULGUR WHEAT, WHOLE WHEAT FLOUR, WHEAT BRAN, YEAST, HONEY, SUGAR, CONTAINS 2% OR LESS OF EACH OF THE FOLLOWING: WHEAT, GLUTEN, SOYBEAN OIL, ROLLED WHEAT, RYE NUGGETS, CORN GRITS, RAISING JUICE CONCENTRATE, SALT, ROLLED OATS, RYE FLAKES, CALCIUM SULFATE, TRITICALE FLAKES, PARBOILED BROWN RICE FLOUR, BARLEY FLAKES, HULLED MILLET, WHOLE FLAX SEED, DOUGH CONDITIONERS (CONTAINS MORE OR ONE OF THE FOLLOWING: SODIUM STEAROYL, LACTYLATE, CALCIUM STEAROYL, LACTYLATE, MONOGLYCERIDES, MONO- AND DIGLYCERIDES, DISTILLED MONOGLYCERIDES, CALCIUM PEROXIDE, CALCIUM IODATE, DATEM, ETHOXYLATED MONO- AND DIGLYCERIDES, ENZYMES, ASCORBIC ACIDS), WHEAT STARCH, CALCIUM PROPIONATE (TO RETARD SPOILAGE), TOPPED WITH ROLLED OATS., Balsamic Red Onions: Red Onion, Balsamic Vinegar (sub-ingredients not available)., Black Cherry Honey Mustard: HONEY MUSTARD DRESSING (MAYONNAISE ...
Calories in Barilla Whole Wheat Pasta Elbow Macaroni. Find nutrition facts for Barilla Whole Wheat Pasta Elbow Macaroni and over 2,000,000 other foods in MyFitnessPal.coms food database.
Citation: SCHISLER, D.A., KHAN, N.I., BOEHM, M.J., VAN CAUWENBERGE, J.E., SLININGER, P.J. DISCOVERY, PILOT-PLANT PRODUCTION, AND EFFICACY OF ANTAGONISTS OF FUSARIUM HEAD BLIGHT (SCAB) OF WHEAT. 8TH INTERNATIONAL CONGRESS OF PLANT PATHOLOGY. ABSTRACT PAPER NUMBER 3.85. Interpretive Summary: Technical Abstract: A serious impediment to the commercialization of biocontrol agents is the lack of adequate methodologies for selecting biocontrol agents that have both high efficacy and amenability to the stresses of commercial-scale biomass production. Seven microbial strains were obtained from wheat anthers and were effective against three different isolates of Gibberella zeae in greenhouse tests. Bacillus subtilis strains AS 43.3, AS 43.4, and Cryptococcus nodaensis OH 182.9 reduced disease severity by ,77%, 93%, and 56%, respectively. Yeasts Cryptococcus nodaensis OH 182.9 and Cryptococcus sp. OH 181.1 reduced disease severity by as much as 60% in a series of field experiments on winter and durum ...
Great buffet snack or party food using our duck style pieces in a hoisin sauce. 8 per pack. Samosa Pastry (Wheat Flour (Flour, Calcium Carbonate, Niacin, Iron, Thiamine), Water, Rapeseed Oil, Salt), Water, Carrots, Spring Onions, Wheat Gluten, Hoisin Sauce (Sugar, Crushed Yellow Beans (Fermented Salted Soya Beans) Soya Beans, Wheat Flour, Salt Water), Soy Sauce (Water, Soya Beans, Salt, Wheat Flour) Sugar, Water, Colour: Plain Caramel, Preservative: Potassium Sorbate), Water, Garlic, Mushroom Extract) Modified Maize Starch, Spice Acetic Acid, Salt) Rapeseed Oil, Non-Hydrogenated Vegetable Fat (Sustainable Palm), Soya Protein, Sugar, Wheat Fibre, Natural Flavouring, Yeast Extract, Salt, Dried Yeast, Spices & Spice Extracts, Preservative: Potassium Sorbate, Thickener: Methyl Cellulose, Garlic Powder, Malt Extract (contains Barley). Nutritional per ...
TY - JOUR. T1 - Bioethanol production from wheat straw by phosphoric acid plus hydrogen peroxide (PHP) pretreatment via simultaneous saccharification and fermentation (SSF) at high solid loadings. AU - Qiu, Jingwen. AU - Tian, Dong. AU - Shen, Fei. AU - Hu, Jinguang. AU - Zeng, Yongmei. AU - Yang, Gang. AU - Zhang, Yanzong. AU - Deng, Shihuai. AU - Zhang, Jing. PY - 2018/11/1. Y1 - 2018/11/1. N2 - Phosphoric acid plus hydrogen peroxide (PHP) pretreatment was employed on wheat straw for ethanol conversion by simultaneous saccharification and fermentation (SSF) at high loadings. Results showed solid loading of PHP-pretreated wheat straw can be greatly promoted to 20%. Although more enzyme input improved ethanol conversion significantly, it still can be potentially reduced to 10-20 mg protein/g cellulose. Increasing yeast input also promoted ethanol conversion, however, the responses were not significant. Response surface method was employed to optimize SSF conditions with the strategy of ...
TY - JOUR. T1 - Hemoglobin transition in erythrocytes of developing chick. Studies with cell-free protein-synthesizing systems. AU - Henderson, A. Burl. AU - Lee, John C.. PY - 1976. Y1 - 1976. N2 - Cell-free hemoglobin-synthesizing systems from erythrocytes of 4- and 17-day chick embryos have been developed. These systems have been used to investigate possible structural and functional differences in factors involved in protein synthesis obtained from these different developmental stages. Each cell-free system consists of three major cellular fractions i.e., the S-100 supernatant, the salt-washed ribosomes, and the 0.5 m KCl ribosomal wash. When the ribosomal wash fraction from one developmental stage is included in a cell-free system containing ribosomes and S-100 supernatant from the other developmental stage, a drastic reduction in the kinetics of [3H]leucine incorporation into globin products is observed, when compared to the homologous control cell-free systems. A similar depression of the ...
0034] The animal feed comprising the ingredient and at least one proteinaceous feed ingredient may comprise a proteinaceous feed ingredient, such as, plant and vegetable proteins, including edible grains and grain meals selected from the group consisting of soybeans, soybean meal, corn, corn meal, linseed, linseed meal, cottonseed, cottonseed meal, rapeseed, rapeseed meal, sorghum protein, and canola meal. Other examples of proteinaceous feed ingredients may include; corn or a component of corn, such as, for example, corn fiber, corn hulls, corn stover, silage, ground corn, or any other portion of a corn plant; soy or a component of soy, such as, for example, soy hulls, soy silage, ground soy, or any other portion of a soy plant; wheat or any component of wheat, such as, for example, wheat fiber, wheat hulls, wheat chaff, ground wheat, wheat germ, or any other portion of a wheat plant; canola or any other portion of a canola plant, such as, for example, canola protein, canola hulls, ground ...
Dear John Dough,. And you thought conditioner was just for hair? Commercial bread bakers often use additives, such as dough conditioners, to improve the texture, appearance, and shelf-life of their bread. Dough conditioners strengthen the gluten (protein portion of wheat) and starch (complex carbohydrate) in the dough. This prevents overmixing of the bread dough, which can easily happen when large quantities of dough are made at one time. Overmixed dough rises irregularly, and thus produces funny shaped, dense loaves. The addition of dough conditioners helps to ensure rounded, well shaped, light loaves.. The most common dough conditioners are sodium stearoyl 2 lactylate, calcium stearoyl lactate, barley malt, ethoxylated and succinylated monoglycerides, and polysorbate 60. The Center for Science in the Public Interest (which is often conservative when it comes to "questionable food items") rated these dough conditioners as "additives that appear to be safe." Dough conditioners are used in very ...
With high,middle and low amylose content rice cultivars as material,influence of weak light during the initial period of young spike on grain-filling and quality in rice was studied.Grain-filling course was simulated by Richards equation.The results showed that,compared with the control, the maximum growth amount of grain(A),brown rice rate and milled rice rate decreased,the change of initial grain-filling potential(R_0),maximum grain-filling rate(G_max),mean grain-filling rate(G),head milled rice rate,chalky grains rate,amylose and protein contents were different in the 3 varieties.Brown rice rate and milled rice rate were significantly and very significantly positively correlated with A.Amylose content and head rice rate were significantly and very significantly negatively correlated with R_0,G_max and G. Chalky grains rate was significantly and very significantly positively correlated with R_0,G_max and G.In order to release the damage of weak light to rice quality,measures for breeding,growth
Gluten free flour is a term that is applied to flours that are made of non-gluten containing products. There are many kinds of gluten free flours available at supermarkets these days, along with many "all purpose" gluten free flour blends that are designed to be an easy to use replacement for wheat flour. Gluten is a protein found in wheat, rye, barley and triticale that, when water or liquid is added to it, makes an elastic dough. Gluten free flours on their own do not have this elasticity and typically produce a much denser product, so blends of different types of grains are used to create gluten free flour mixes with a more versatile consistency that will work well in the same applications as wheat flour.. Commercially available gluten free flours are all made with different mixtures and these mixtures vary widely from brand to brand. They might contain rice flour, teff flour, tapioca flour, sorghum flour, potato starch, garbanzo flour or buckwheat flour - just to name a few of the many ...
Lolium perenne ssp. multiflorum (Italian ryegrass) is a major weed problem in wheat production. Being an obligate outcrossing species, it has a high propensity to evolve resistance to herbicides. This study was conducted to determine the level of resistance and resistance patterns of Italian ryegrass populations in the United States to ACCase- (diclofop and pinoxaden) and ALS (imazamox, mesosulfuron, and pyroxsulam) inhibitors. Dose-response bioassays were conducted on 47 populations from suspect herbicide-resistant fields in the southern United States (Arkansas, Mississippi, Georgia, North Carolina, South Carolina, Virginia), Kansas, and Washington collected between 2008 and 2011 from fields suspected of resistance to ALS inhibitors. Eighty-seven percent of the populations were resistant to diclofop. Eight of the diclofop-resistant populations (25%) were also resistant to pinoxaden. Thirty-eight diclofop-resistant populations (81%) were resistant to at least one ALS inhibitor. Thirty-nine ...
Lolium perenne ssp. multiflorum (Italian ryegrass) is a major weed problem in wheat production. Being an obligate outcrossing species, it has a high propensity to evolve resistance to herbicides. This study was conducted to determine the level of resistance and resistance patterns of Italian ryegrass populations in the United States to ACCase- (diclofop and pinoxaden) and ALS (imazamox, mesosulfuron, and pyroxsulam) inhibitors. Dose-response bioassays were conducted on 47 populations from suspect herbicide-resistant fields in the southern United States (Arkansas, Mississippi, Georgia, North Carolina, South Carolina, Virginia), Kansas, and Washington collected between 2008 and 2011 from fields suspected of resistance to ALS inhibitors. Eighty-seven percent of the populations were resistant to diclofop. Eight of the diclofop-resistant populations (25%) were also resistant to pinoxaden. Thirty-eight diclofop-resistant populations (81%) were resistant to at least one ALS inhibitor. Thirty-nine ...
Banana flour is a powder traditionally made of green bananas. Historically, banana flour has been used in Africa and Jamaica as a cheaper alternative to wheat flour. It is now often used as a gluten free replacement to wheat flours or as a source of resistant starch, which has been promoted by certain dieting trends such as paleo and primal diets and by some recent nutritional research. Banana flour, due to the use of green bananas, has a very mild banana flavor raw and, when cooked, it has an earthy non-banana flavor; it also has a texture reminiscent of lighter wheat flours and requires about 25% less volume, making it a good replacement for white and white whole wheat flour. This has led to rising popularity among those suffering from celiac disease and gluten free dieters. Banana flour is generally produced with green bananas that are peeled, chopped, dried, and then ground. This process can be completed traditionally by hand where the bananas are sun dried, dried in an oven, or a ...
Seedlings of the annual grass Vulpia ciliata ssp. ambigua were grown in sterilized sand with a dark septate root fungus, Phialophora graminicola, which had been isolated from a natural population of the grass. Tiller number and shoot, root and total biomass of seedlings grown with P. graminicola were enhanced relative to uninoculated control plants in a growth room and a glasshouse experiment. Root length of seedlings grown with P. graminicola was significantly increased, but no effects of the fungus on root diameter, number of root hairs or specific root length were recorded. Root nitrogen content and shoot, root and total phosphorus contents of seedlings grown with the fungus were enhanced, but shoot nitrogen concentration of these plants was reduced. Shoot biomass and specific root length of inoculated plants were positively associated with the number of P. graminicola colonies re-isolated from roots. These data indicate that P. graminicola acts as a beneficial associate of V. ciliata ssp. ...
Sourdough fermentation is considered to have beneficial effects on postprandial satiety and metabolic responses, but studies demonstrating effects at physiological conditions are lacking. The aim of this acute breakfast intervention study was to determine the effect of consumption of sourdough-fermented and unfermented rye crispbread on self-rated appetite, postprandial glucose and insulin response in healthy subjects. In all, twenty-four Swedish adults were included in a single-blinded, randomised cross-over trial. Three crispbreads (sourdough-fermented and unfermented whole grain rye and yeast-fermented refined wheat as control) were consumed as part of a standardised breakfast. Subjective appetite score, assessed using visual analogue scale, and plasma glucose and insulin concentrations were measured at baseline and postprandially until 360 and 240 min, respectively. Structural changes and viscosity during mastication and gastric digestion were investigated using in vitro methods. Hunger and ...
In order to identify antifungal compounds from natural sources to be used as ingredients in the bakery industry, water/salt-soluble extracts (WSE) from different legume flour hydrolysates obtained by the use of a fungal protease were assayed against Penicillium roqueforti DPPMAF1. The agar diffusion assays allowed the selection of the pea (Pisum sativum) hydrolysate as the most active. As shown by the hyphal radial growth rate, the WSE had inhibitory activity towards several fungi isolated from bakeries. The MIC of the WSE was 9.0 mg/ml. Fungal inhibition was slightly affected by heating and variations in pH. The antifungal activity was attributed to three native proteins (pea defensins 1 and 2 and a nonspecific lipid transfer protein [nsLTP]) and a mixture of peptides released during hydrolysis. The three proteins have been reported previously as components of the defense system of the plant. Five peptides were purified from WSE and were identified as sequences encrypted in leginsulin A, ...
The relationship between the production of extracellular H2O2, hydrogen peroxide-producing enzymes and ligninolytic peroxidase was examined during solid-state cultivation ofPanus tigrinus on wheat straw. Glyoxal oxidase, Mn2+-dependent peroxidase and glucose oxidase, capable of H2O2 generation, were found in the extracellular enzyme preparation. The production of H2O2 has two maxima: the maximal production correlates well with the maximal activities of glyoxal oxidase and Mn2+-dependent peroxidase, while another, lower peak of H2O2 generation is related to the second peak of Mn2+-dependent peroxidase activity. The contribution of glucose oxidase to the production of hydrogen peroxide is probably only marginal. Comparison of the dynamics of these extracellular activities and the ligninolytic peroxidase showed good temporal correlation indicating an interrelation of the two processes.
This datatable is part of the Living Field Laboratory dataset. The Living Field Laboratory is a long-term rotation study established in 1993 under the direction of Emeritus Professor Dr. Richard Harwood to evaluate the benefits of cover crops (as green manure) and/or the addition of composted dairy manure in two integrated systems compared to a conventional and an organic system. The LFL accommodates every entry point of the crop rotation each year and has a factorial design that allows for the comparison of a number of interactions. These interactions include: differences in N management, rotation effects, and cover crops. From 1993 through 2005, the rotation consisted of corn (Zea mays L.)/corn/soybean (Glycine max)/wheat (Triticum aestivum) vs. continuous corn. During this period, crimson clover (Trifolium incarnatum L.) was seeded to 1st yr. corn, 2nd yr corn was in rye grass (Lolium multiflorum), soybean had no cover, and red clover (Trifolium pretense L.) was frost-seeded into ...

Role of Glycogen Synthase Kinase-3β in Neuronal Apoptosis Induced by Trophic Withdrawal | Journal of NeuroscienceRole of Glycogen Synthase Kinase-3β in Neuronal Apoptosis Induced by Trophic Withdrawal | Journal of Neuroscience

... nuclei fragmentation or condensation, and DNA cleavage into oligonucleosome fragments manifested as "DNA laddering," hallmarks ... Arrows indicate healthy cells with uniformly stained nuclei. Arrowheads identify cells with apoptotic morphology, including ... B, DNA fragmentation manifested as a DNA ladder. Cortical neurons were treated with serum deprivation with exposure to MK-801 ... Uniformly stained nuclei were scored as healthy, viable neurons. Condensed or fragmented nuclei were scored as apoptotic. To ...
more infohttps://www.jneurosci.org/content/20/7/2567?ijkey=780a9439a6ccd8727b55370a331ec8b7df65045d&keytype2=tf_ipsecsha

Anti-Mouse (Murine) DFFB antibody for Immunofluorescence (Paraffin-embedded Sections) (IF (p))Anti-Mouse (Murine) DFFB antibody for Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Degrades naked DNA and induces apoptotic morphology.. Subcellular location: Cytoplasm, Nucleus. Synonyms: CAD, CPAN, 40 kDa, ... DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, Caspase-activated DNase, DNA fragmentation factor ... DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies show synonyms for this antigen * ... DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Alternative Name DFFB (DFFB Antibody ...
more infohttps://www.antibodies-online.com/apoptosis-pathway-9/dffb-antibody-7629/mouse-if-p-29687/

Neuroprotective effect of ZnT3 knockout on subarachnoid hemorrhage : Translational NeuroscienceNeuroprotective effect of ZnT3 knockout on subarachnoid hemorrhage : Translational Neuroscience

Conversely, the improvement of behavior in the KO-SAH group was associated with a marked reduction in apoptotic neurons. ... SAH mice and their poor neurological score was accompanied by an increase in the number of apoptotic neurons. ... Condensed nuclei and shrunken cells represent the typical apoptotic cell morphology, and were indicated by the blue arrows. ∗, ... TUNEL assay showed neurons with DNA fragmentation (magnification, 20X). (A) WT-CON; (B) WT-SAH; (C) KO-SAH group; (D) ...
more infohttps://www.degruyter.com/view/j/tnsci.2018.9.issue-1/tnsci-2018-0006/tnsci-2018-0006.xml?rskey=TS1usU&result=66

Myeloperoxidase Delays Neutrophil Apoptosis Through CD11b/CD18 Integrins and Prolongs Inflammation | Circulation ResearchMyeloperoxidase Delays Neutrophil Apoptosis Through CD11b/CD18 Integrins and Prolongs Inflammation | Circulation Research

Middle, Arrows indicate typically apoptotic morphology, showing condensed nuclei. D, MPO attenuates DNA fragmentation in ... The percentage of annexin V-positive neutrophils (Figure 6E), DNA fragmentation (Figure 6F) and caspase-3 activity (Figure 6G) ... Cell viability and staining with annexin V (C), and DNA fragmentation and caspase-3 activity (D) were assayed immediately after ... DNA cleavage was shown by detection of cytoplasmic histone-associated DNA fragments with Cell Death ELISA (Roche) and gel ...
more infohttp://circres.ahajournals.org/content/103/4/352

MUL1 - WikipediaMUL1 - Wikipedia

... and fragmentation of the DNA and nucleus. This is followed by fragmentation into apoptotic bodies that are quickly removed by ... where it regulates mitochondrial morphology and apoptosis. Specifically, this enzyme has pro-apoptotic functions. An apoptotic ... Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial ... Can also activate NF-κB to initiate mitochondria-to-nucleus signaling under stress. As aforementioned, MUL1 encodes for an ...
more infohttps://en.wikipedia.org/wiki/MUL1

Identification of a nuclear-localized nuclease from wheat cells undergoing programmed cell death that is able to trigger DNA...Identification of a nuclear-localized nuclease from wheat cells undergoing programmed cell death that is able to trigger DNA...

Moreover, nuclear extracts from apoptotic human cells triggered DNA fragmentation and apoptotic morphology in nuclei from plant ... Furthermore, we show that the nuclear extracts from plant cells triggered DNA fragmentation and apoptotic morphology in nuclei ... Nuclear extracts from nucellar cells undergoing PCD triggered DNA fragmentation and other apoptotic morphology in nuclei from ... from wheat cells undergoing programmed cell death that is able to trigger DNA fragmentation and apoptotic morphology on nuclei ...
more infohttp://www.biochemj.org/content/397/3/529

Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South...Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South...

DNA isolation and gel electrophoresis. Apoptotic DNA fragments were analyzed using the procedure described by Ahmad et al. (1 ... chromatin condensation and nucleus fragmentation (Figure 3B), and after 24 hours, nuclei fragmentation increased (Figure 3C). ... Our results indicated that a factor present in C. d. terrificus venom also produced notable changes in the cells morphology. ... Detectable morphological changes in the nucleus are chromatin condensation and, at a later stage, fragmentation of the nucleus ...
more infohttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100004

Frontiers | Evaluation of Apoptosis and Autophagy Inducing Potential of Berberis aristata, Azadirachta indica, and Their...Frontiers | Evaluation of Apoptosis and Autophagy Inducing Potential of Berberis aristata, Azadirachta indica, and Their...

A DNA marker was run parallel to the samples.. DAPI Staining of Nucleus. For detection of apoptotic nuclei by 4′-6-diamidino-2- ... Analysis of DNA Fragmentation. A distinctive biochemical feature of apoptosis induction is the fragmentation of genomic DNA by ... Nuclear morphology was then observed by fluorescence microscopy (Olympus, Japan). Apoptotic nuclei can be identified by the ... One of the classical methods used to detect DNA ladders is to examine fragmented genomic DNA on an agarose gel. To analyze DNA ...
more infohttps://www.frontiersin.org/articles/10.3389/fonc.2017.00296/full

The Relationship between Apoptosis and High-Mobility Group Protein 1 Release from Murine Macrophages Stimulated with...The Relationship between Apoptosis and High-Mobility Group Protein 1 Release from Murine Macrophages Stimulated with...

... cells lacking HMGB1 nuclear staining also demonstrated condensation or fragmentation of DNA as shown with staining with DRAQ5. ... the translocation of HMGB1 from the nucleus into the extracellular milieu under conditions in which the frequency of apoptotic ... Confocal imaging was performed next to evaluate HMGB1 translocation and to analyze the morphology of cells undergoing this ... As shown in a previous study, lipoteichoic acid, poly(I:C), and LPS but not CpG DNA can induce the release of HMGB1 from RAW ...
more infohttps://www.jimmunol.org/content/178/10/6495?ijkey=175a5a5af460bfd0df7e1b4c7a456fdc6415b166&keytype2=tf_ipsecsha

The Relationship between Apoptosis and High-Mobility Group Protein 1 Release from Murine Macrophages Stimulated with...The Relationship between Apoptosis and High-Mobility Group Protein 1 Release from Murine Macrophages Stimulated with...

... cells lacking HMGB1 nuclear staining also demonstrated condensation or fragmentation of DNA as shown with staining with DRAQ5. ... the translocation of HMGB1 from the nucleus into the extracellular milieu under conditions in which the frequency of apoptotic ... Confocal imaging was performed next to evaluate HMGB1 translocation and to analyze the morphology of cells undergoing this ... As shown in a previous study, lipoteichoic acid, poly(I:C), and LPS but not CpG DNA can induce the release of HMGB1 from RAW ...
more infohttps://www.jimmunol.org/content/178/10/6495?ijkey=6c5335d17a2bce437be6cbe50f0bd310c7f3b9e8&keytype2=tf_ipsecsha

Plus itPlus it

Apoptotic cells are depicted by the images in the panels in C (DNA fragmentation) and D (immunoreactive cleaved caspase-3 cells ... and their identification was based on the morphology and color of cytoplasm, nucleus, and granulations. Every sixth section of ... Apoptotic cells are depicted by the images in C (DNA fragmentation) and D (immunoreactive cleaved caspase-3 cells). The ... This was also the case for apoptotic cells that were labeled via a TdT-FragEL DNA fragmentation detection kit and by ...
more infohttp://www.jneurosci.org/content/23/13/5536

Combination of CHS 828 and etoposide in vitro - from cytotoxic synergy to complete inhibition of apoptosisCombination of CHS 828 and etoposide in vitro - from cytotoxic synergy to complete inhibition of apoptosis

The cells exhibited apoptotic morphology, including condensed and fragmented nuclei and formation of apoptotic bodies, ... activation of caspase 3 and 8, and DNA fragmentation, visualised by TdT-mediated dUTP nick end-labelling (TUNEL). ... In summary, CHS 828 induced cell death is predominantly non-apoptotic, does not involve caspases and can be postponed by ... After ~30 h, proliferation (DNA synthesis) and protein synthesis ceased, and viability started to decrease towards 10% at 72 h ...
more infohttp://uu.diva-portal.org/smash/record.jsf?pid=diva2:161085

Plus itPlus it

A, apoptotic nuclear morphology in staurosporine (STS)-induced M7 and Neo cells. Nuclear DNA was stained with Hoechst (blue). ... an exclusive apoptotic cytokine (25) . Ninety min into treatment, ,70% of M7 cells showed nuclear DNA fragmentation to various ... Apoptotic cells with fragmented nuclei as visualized by Hoechst staining were presented as a percentage of the total number of ... PARP cleavage and DNA fragmentation). Bax overexpression may further predict an elevated cellular apoptotic response to a broad ...
more infohttp://cancerres.aacrjournals.org/content/64/5/1703

Molecules  | Free Full-Text | ROS-Dependent Antiproliferative Effect of Brassinin Derivative Homobrassinin in Human Colorectal...Molecules | Free Full-Text | ROS-Dependent Antiproliferative Effect of Brassinin Derivative Homobrassinin in Human Colorectal...

Apoptosis was also confirmed by annexin V/PI double staining, DNA fragmentation assay and chromatin condensation assay. The ... Furthermore, simultaneously with G2/M arrest, K1 also increased population of cells with sub-G1 DNA content which is considered ... to be a marker of apoptotic cell death. ... and stained with DAPI to show typical apoptotic morphology. ... of three independent experiments are shown as the apoptotic index evaluated as a percentage of cells with fragmented nuclei ...
more infohttps://www.mdpi.com/1420-3049/19/8/10877/htm

Role of the Mitochondrial Permeability Transition Pore in Apoptosis | Bioscience Reports | Portland PressRole of the Mitochondrial Permeability Transition Pore in Apoptosis | Bioscience Reports | Portland Press

... including characteristic changes in nuclear morphology and biochemistry (chromatin condensation and DNA fragmentation), depends ... Recent data suggest that the acquisition of the apoptotic phenotype, ... the nucleus, and the plasma membrane; (iv) PT is modulated by members of the apoptosis-regulatory bcl-2 gene family. ...
more infohttps://portlandpress.com/bioscirep/article/17/1/67/54153/Role-of-the-Mitochondrial-Permeability-Transition

Pseudomonas aeruginosa Cystic Fibrosis Isolates Induce Rapid, Type III Secretion-Dependent, but ExoU-Independent, Oncosis of...Pseudomonas aeruginosa Cystic Fibrosis Isolates Induce Rapid, Type III Secretion-Dependent, but ExoU-Independent, Oncosis of...

In conclusion, the absence of DNA fragmentation, observations of Hoechst-stained nuclei, and the TEM studies have enabled us to ... or late apoptotic morphology with the nucleus having the same profile as in early apoptosis but dissolution of the cytoplasm (E ... 1993) Microscale autoradiographic method for the qualitative and quantitative analysis of apoptotic DNA fragmentation. J. Cell ... DNA fragmentation assays. (A) DNA was isolated from J774 macrophages or PMNs and migrated on a 1.5% agarose gel. Lanes: 1, ...
more infohttps://iai.asm.org/content/68/5/2916?ijkey=43a4e8195fc090694ef250c4c5ccccb885de222f&keytype2=tf_ipsecsha

Hyperforin Inhibits Cancer Invasion and Metastasis | Cancer ResearchHyperforin Inhibits Cancer Invasion and Metastasis | Cancer Research

3E ⇓ ), and late apoptosis (DNA fragmentation) detected by terminal deoxynucleotidyl transferase-mediated nick end labeling ... Hyp-DCHA-Induced Apoptotic Cell Death in HT-1080.. As previously reported (7) , inhibition of tumor cells growth by Hyp-DCHA ... and the cell nuclei were stained with Hoechst 33258; the samples were then analyzed by fluorescence microscopy. ... we now report that HT-1080 cells treated for 7 hours with 9 μmol/L Hyp-DCHA show typical alterations of membrane morphology ( ...
more infohttp://cancerres.aacrjournals.org/content/64/17/6225?ijkey=98ad8bbb57cf98b063ac5b7d59b32d4eccd2a0ad&keytype2=tf_ipsecsha

Sensitivity to Apoptosis Signal, Clearance Rate, and Ultrastructure of Fas Ligand-Induced Apoptosis in In Vivo Adult Cardiac...Sensitivity to Apoptosis Signal, Clearance Rate, and Ultrastructure of Fas Ligand-Induced Apoptosis in In Vivo Adult Cardiac...

In each cell type, however, the incidence of apoptosis suggested by DNA fragmentation and apoptotic morphology peaked 12 hours ... In the hearts of group F5, myocytes were observed that were accompanied by pyknotic nuclei and fragmentation of the nuclei at 6 ... DNA fragmentation and apoptotic morphology) depends on the organs and/or cell types, whereas the time interval from the ... Apoptotic DNA fragmentation occurs by activation of DNase, such as caspase-3-dependent DNase, via caspase-3 activation and ...
more infohttp://circ.ahajournals.org/content/105/25/3039

CFTR mediates apoptotic volume decrease and cell death by controlling glutathione efflux and ROS production in cultured mice...CFTR mediates apoptotic volume decrease and cell death by controlling glutathione efflux and ROS production in cultured mice...

DNA fragmentation is a feature of cystic fibrosis epithelial cells: a disease with inappropriate apoptosis? FEBS Lett 408: 225- ... Under control conditions, the nuclei of wild-type cells excluded propidium iodide and exhibited a normal morphology, with ... Apoptotic cells were counted by comparing the three stains. A cell was considered apoptotic only if the nucleus was not stained ... Apoptotic nuclei were counted in 100-150 orcein-stained cells and expressed as a percentage of total cells. Values are means ± ...
more infohttp://ajprenal.physiology.org/content/298/2/F435

Fishing for Answers: Autism, Genes and Mercury. - AGE OF AUTISMFishing for Answers: Autism, Genes and Mercury. - AGE OF AUTISM

Apoptotic morphology was detected in thimerosal-treated cells. Figure 5 demonstrates that, after 6 h of incubation, both ... large DNA fragmentation and condensation of chromatin.23 Since thimerosal also causes AIF release from the mitochondria, it is ... A considerable accumulation of this metal into cell nuclei of both the examined organs was observed (1.9 and 0.1 μg Hg/mg ... As the chromatin fibres contain the double helix of DNA, this assumption would imply that parts of the DNA molecule might be ...
more infohttps://www.ageofautism.com/2010/09/fishing-for-answers-autism-genes-and-mercury.html?cid=6a00d8357f3f2969e20134873c709c970c

Apoptosis - WikipediaApoptosis - Wikipedia

Nagata S (April 2000). "Apoptotic DNA fragmentation". Exp. Cell Res. 256 (1): 12-8. doi:10.1006/excr.2000.4834. PMID 10739646. ... The nucleus breaks into several discrete chromatin bodies or nucleosomal units due to the degradation of DNA. Apoptosis ... In order to perform analysis of apoptotic versus necrotic (necroptotic) cells, one can do analysis of morphology by time-lapse ... Anoikis Apaf-1 Apo2.7 Apoptotic DNA fragmentation Atromentin induces apoptosis in human leukemia U937 cells. Autolysis ...
more infohttps://en.wikipedia.org/wiki/Apoptosis

Amnis Imaging Flow Cytometers | Life Science Research | Merck | Research Area - OncologyAmnis Imaging Flow Cytometers | Life Science Research | Merck | Research Area - Oncology

Apoptotic Index Using the ImageStream. DNA condensation and fragmentation are hallmarks of apoptosis. By measuring the area and ... healthy nucleus. This makes possible the automated identification of apoptotic nuclear morphology. See the application note for ... When cells begin to dye by apoptosis there is fragmentation and condensation of the DNA. By measuring the area and the ... but also to identify the fragmented nuclei of apoptotic cells treated with a chemotherapeutic drug, or to visualize the binding ...
more infohttp://www.merckmillipore.com/RO/ro/life-science-research/cell-analysis-flow-cytometry/amnis-imaging-flow-cytometers/research-areas/oncology/GNGb.qB.fokAAAFLl95T0Lxx,nav

Amnis Imaging Flow Cytometers | Life Science Research | Merck | Research Area - OncologyAmnis Imaging Flow Cytometers | Life Science Research | Merck | Research Area - Oncology

Apoptotic Index Using the ImageStream. DNA condensation and fragmentation are hallmarks of apoptosis. By measuring the area and ... healthy nucleus. This makes possible the automated identification of apoptotic nuclear morphology. See the application note for ... When cells begin to dye by apoptosis there is fragmentation and condensation of the DNA. By measuring the area and the ... but also to identify the fragmented nuclei of apoptotic cells treated with a chemotherapeutic drug, or to visualize the binding ...
more infohttp://www.merckmillipore.com/BR/pt/life-science-research/cell-analysis-flow-cytometry/amnis-imaging-flow-cytometers/research-areas/oncology/GNGb.qB.fokAAAFLl95T0Lxx,nav

Sensory Neurons With Activated Caspase-3 Survive Long-Term Experimental Diabetes | DiabetesSensory Neurons With Activated Caspase-3 Survive Long-Term Experimental Diabetes | Diabetes

A marker of nuclei, 4′,6-diamidino-2-phenylindole (DAPI), was used to counterstain DNA. After immunohistochemical labeling, ... Sensory neurons are not labeled by TUNEL in diabetes and do not have apoptotic nuclear morphology.. Assuming that the ... None of the neuronal nuclei had chromatin condensation or fragmentation to suggest apoptosis, and cytoplasmic blebbing ... Apoptotic cell death detection (transferase-mediated dUTP nick-end labeling technology).. Apoptotic cell death was detected by ...
more infohttps://diabetes.diabetesjournals.org/content/52/9/2363?ijkey=230f967e11e23f00cff860ef39865d319f5b7442&keytype2=tf_ipsecsha

Upregulation of Bax and Bcl-2 following prenatal cocaine exposure induces apoptosis in fetal rat brainUpregulation of Bax and Bcl-2 following prenatal cocaine exposure induces apoptosis in fetal rat brain

Cocaine-induced nucleosomal DNA fragmentation in fetal brain was separated in 1.8% agarose gels. The apoptotic DNA ladders ... The nuclear morphology was examined by fluorescence microscopy. Individual nuclei were visualized at ×400, and cells were ... DNA ladders in fetal rat brains were examined as previously described using the apoptotic DNA ladder kit from Sigma [8, 9]. DNA ... 2). Quantification of cocaine-induced apoptotic nuclei defined by the fluorescent DNA-binding dye Hoechst 33258 indicated that ...
more infohttp://www.medsci.org/v05p0295.htm
  • The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. (scielo.br)
  • Specific nucleosomal DNA fragmentation after the UVB irradiation was detected also using agarose gel electrophoresis. (radioprotection.org)
  • While conventional in situ detection techniques such as ISEL (Klenow DNA polymerase), TUNEL (terminal deoxynucleotidyl transferase, TdT) and ISNT (DNA Polymerase I) are useful in detecting internucleosomal DNA cleavage, they do not differentiate DNase Type I and DNase Type II cleavage which results from the activation of apoptotic endonucleases. (emdmillipore.com)
  • Neurons were subjected to several apoptotic paradigms, including serum deprivation, serum deprivation combined with exposure to NMDA receptor antagonists, or treatment with PI-3 kinase inhibitors. (jneurosci.org)
  • The Wild Type (WT)-SAH group behaved poorly when compared to the knockout (KO)-SAH mice and their poor neurological score was accompanied by an increase in the number of apoptotic neurons. (degruyter.com)
  • Conversely, the improvement of behavior in the KO-SAH group was associated with a marked reduction in apoptotic neurons. (degruyter.com)
  • Despite this confirmation that sensory neurons survive, neurons had elevated expression of activated caspase-3 in unique patterns that included their nuclei, cytoplasm, and proximal axonal segments. (diabetesjournals.org)
  • In contrast, diabetic sensory neurons had elevated expression of the DNA repair enzyme poly(ADP-ribose) polymerase (PARP) in their nuclei, cytoplasm, and proximal axonal segments not overlapping with caspase-3 localization. (diabetesjournals.org)
  • Inside the cell, HMGB1 binds to DNA and promotes transcription as well as other functions ( 1 , 2 ). (jimmunol.org)
  • Specifically, this enzyme has pro-apoptotic functions. (wikipedia.org)
  • The ApopTag ISOL Dual Fluorescence Kit utilizes a proprietary double hairpin, dual fluorescently labeled oligonucleotide labeling process to detect and distinguish between typical apoptotic DNA breaks induced by either DNase I or DNase II enzyme activities. (emdmillipore.com)
  • After ~30 h, proliferation (DNA synthesis) and protein synthesis ceased, and viability started to decrease towards 10% at 72 h. (diva-portal.org)
  • In summary, CHS 828 induced cell death is predominantly non-apoptotic, does not involve caspases and can be postponed by maintained protein synthesis and ATP levels. (diva-portal.org)
  • The distinguishing feature of aroids is the occasionally beautiful and often bizarre combination of spathe and spadix called the inflorescenceis, which is used for trapping their pollinators because of their particular morphology and organization of their inflorescences [ 1 ]. (hindawi.com)
  • In contrast, normal or proliferative nuclei, which have relatively insignificant numbers of DNA 3'-OH ends, usually do not stain with the kit. (emdmillipore.com)
  • CPP32, a Novel Human Apoptotic Protein with Homology to Caenorhabditis elegans Cell Death Protein Ced-3 and Mammalian Interleukin-1.beta. (patentgenius.com)
  • Can also activate NF-κB to initiate mitochondria-to-nucleus signaling under stress. (wikipedia.org)
  • Interestingly, it is now well established that ion channels responsible for RVD are also involved in the induction of apoptotic volume decrease (AVD), which is an early event in apoptotic cell death. (physiology.org)
  • We suggest that deregulation of the control of chromatin assembly might contribute to development of tumors in tuberous sclerosis patients and provide important new insights into the molecular development, especially since deregulation of chromatin assembly and DNA repair results in genomic instability, a hallmark of tumor development. (ageofautism.com)
  • In addition to its importance as a biological phenomenon, defective apoptotic processes have been implicated in a wide variety of diseases. (wikipedia.org)
  • The DNA strand breaks are detected by enzymatically labeling the free 3'-OH termini with modified nucleotides. (emdmillipore.com)