Apoferritins
The protein components of ferritins. Apoferritins are shell-like structures containing nanocavities and ferroxidase activities. Apoferritin shells are composed of 24 subunits, heteropolymers in vertebrates and homopolymers in bacteria. In vertebrates, there are two types of subunits, light chain and heavy chain. The heavy chain contains the ferroxidase activity.
Ferritins
Iron-containing proteins that are widely distributed in animals, plants, and microorganisms. Their major function is to store IRON in a nontoxic bioavailable form. Each ferritin molecule consists of ferric iron in a hollow protein shell (APOFERRITINS) made of 24 subunits of various sequences depending on the species and tissue types.
Iron
Encyclopedias as Topic
Neurodegenerative Diseases
Pseudogenes
Genes bearing close resemblance to known genes at different loci, but rendered non-functional by additions or deletions in structure that prevent normal transcription or translation. When lacking introns and containing a poly-A segment near the downstream end (as a result of reverse copying from processed nuclear RNA into double-stranded DNA), they are called processed genes.
Overexpression of the hereditary hemochromatosis protein, HFE, in HeLa cells induces and iron-deficient phenotype. (1/232)
A transfectant HeLa cell clone expressing HFE under the control of a tetracycline-repressible promoter was generated. HFE expression was fully repressed by the presence of doxycycline, while it was strongly induced by growth in the absence of doxycycline. HFE accumulation was accompanied by a large (approximately 10-fold) decrease in H- and L-ferritin levels, by a approximately 3-4-fold increase in transferrin receptor, and a approximately 2-fold increase in iron regulatory protein activity. These indices of cellular iron deficiency were reversed by iron supplementation complexes. The overexpressed HFE immunoprecipitated together with transferrin receptor, indicating a physical association which is the likely cause for the observed approximately 30% decrease in 55Fe-transferrin incorporation after 18 h incubation. In the HFE-expressing cells the reduction in transferrin-mediated iron incorporation was partially compensated by a approximately 30% increase in non-transferrin iron incorporation from 55Fe-NTA, evident after prolonged, 18 h, incubations. The findings indicate that HFE binding to transferrin receptor reduces cellular iron availability and regulates the balance between transferrin-mediated and non-transferrin-mediated cellular iron incorporation. (+info)Iron primes hepatic macrophages for NF-kappaB activation in alcoholic liver injury. (2/232)
NF-kappaB activation induced by lipopolysaccharide (LPS) in cultured hepatic macrophages (HM) may be abrogated by pretreatment of cells with a lipophilic iron chelator, 1,2-dimethyl-3-hydroxypyrid-4-one (L1, deferiprone), suggesting a role for iron in this molecular event [M. Lin, M., R. A. Rippe, O. Niemela, G. Brittenham, and H. Tsukamoto, Am. J. Physiol. 272 (Gastrointest. Liver Physiol. 35): G1355-G1364, 1997]. To ascertain the relevance in vivo of this hypothesis, HM from an experimental model of alcoholic liver injury were examined for the relationship between nuclear factor (NF)-kappaB activation and iron storage. HM showed a significant increase in nonheme iron concentration (+70%), accompanied by enhanced generation of electron paramagnetic resonance-detected radicals (+200%), NF-kappaB activation (+100%), and tumor necrosis factor-alpha (+150%) and macrophage inflammatory protein-1 (+280%) mRNA induction. Treatment of the cells ex vivo with L1 normalized all these parameters. HM content of ferritin protein, ferritin L chain mRNA, and hemeoxygenase-1 mRNA and splenic content of nonheme iron were increased, suggesting enhanced heme turnover as a cause of the increased iron storage and NF-kappaB activation. To test this possibility, increased iron content in HM was reproduced in vitro by phagocytosis of heat-treated red blood cells. Treatment caused a 40% increase in nonheme iron concentration and accentuated LPS-induced NF-kappaB activation twofold. Both effects could be abolished by pretreatment of cells with zinc protoporphyrin, a hemeoxygenase inhibitor. To extend this observation, animals were splenectomized before 9-wk alcohol feeding. Splenectomy resulted in further increments in HM nonheme iron storage (+60%) and NF-kappaB activation (+90%) and mononuclear cell infiltration (+450%), particularly around the iron-loaded HM in alcohol-fed animals. These results support the pivotal role of heme-derived iron in priming HM for NF-kappaB activation and expression of proinflammatory genes in alcoholic liver injury. (+info)Interactions and aggregation of apoferritin molecules in solution: effects of added electrolytes. (3/232)
We have studied the structure of the protein species and the protein-protein interactions in solutions containing two apoferritin molecular forms, monomers and dimers, in the presence of Na(+) and Cd(2+) ions. We used chromatographic, and static and dynamic light scattering techniques, and atomic force microscopy (AFM). Size-exclusion chromatography was used to isolate these two protein fractions. The sizes and shapes of the monomers and dimers were determined by dynamic light scattering and AFM. Although the monomer is an apparent sphere with a diameter corresponding to previous x-ray crystallography determinations, the dimer shape corresponds to two, bound monomer spheres. Static light scattering was applied to characterize the interactions between solute molecules of monomers and dimers in terms of the second osmotic virial coefficients. The results for the monomers indicate that Na(+) ions cause strong intermolecular repulsion even at concentrations higher than 0.15 M, contrary to the predictions of the commonly applied Derjaguin-Landau-Verwey-Overbeek theory. We argue that the reason for such behavior is hydration force due to the formation of a water shell around the protein molecules with the help of the sodium ions. The addition of even small amounts of Cd(2+) changes the repulsive interactions to attractive but does not lead to oligomer formation, at least at the protein concentrations used. Thus, the two ions provide examples of strong specificity of their interactions with the protein molecules. In solutions of the apoferritin dimer, the molecules attract even in the presence of Na(+) only, indicating a change in the surface of the apoferritin molecule. In view of the strong repulsion between the monomers, this indicates that the dimers and higher oligomers form only after partial denaturation of some of the apoferritin monomers. These observations suggest that aggregation and self-assembly of protein molecules or molecular subunits may be driven by forces other than those responsible for crystallization and other phase transitions in the protein solution. (+info)The role of lactoferrin in the bactericidal function of polymorphonuclear leucocytes. (4/232)
Rabbit polymorphonuclear leucocytes contain the iron-binding protein lactoferrin, and can rapidly phagocytose and destroy Pseudomonas aeruginosa. The lactoferrin normally has a large unsaturated iron-binding capacity. If the cells are exposed to a ferritin-antibody complex, large amounts of this are phagocytosed and appear in the cytoplasmic granules and phagosomes. This leads to saturation of the cellular iron-binding protein with Fe. In these circumstances, the bactericidal power of the cells is greatly reduced with the result that some phagocytosed bacteria survive and eventually grow and destroy the cells. An apoferritin-antibody complex used as a control is also phagocytosed but has no effect on the bactericidal power of the cell. The results support the view that lactoferrin plays an essential role in the bactericidal power of the cell. (+info)Overexpression of wild type and mutated human ferritin H-chain in HeLa cells: in vivo role of ferritin ferroxidase activity. (5/232)
Transfectant HeLa cells were generated that expressed human ferritin H-chain wild type and an H-chain mutant with inactivated ferroxidase activity under the control of the tetracycline-responsive promoter (Tet-off). The clones accumulated exogenous ferritins up to levels 14-16-fold over background, half of which were as H-chain homopolymers. This had no evident effect in the mutant ferritin clone, whereas it induced an iron-deficient phenotype in the H-ferritin wild type clone, manifested by approximately 5-fold increase of IRPs activity, approximately 2.5-fold increase of transferrin receptor, approximately 1.8-fold increase in iron-transferrin iron uptake, and approximately 50% reduction of labile iron pool. Overexpression of the H-ferritin, but not of the mutant ferritin, strongly reduced cell growth and increased resistance to H(2)O(2) toxicity, effects that were reverted by prolonged incubation in iron-supplemented medium. The results show that in HeLa cells H-ferritin regulates the metabolic iron pool with a mechanism dependent on the functionality of the ferroxidase centers, and this affects, in opposite directions, cellular growth and resistance to oxidative damage. This, and the finding that also in vivo H-chain homopolymers are much less efficient than the H/L heteropolymers in taking up iron, indicate that functional activity of H-ferritin in HeLa cells is that predicted from the in vitro data. (+info)Hydrogen ion interactions of horse spleen ferritin and apoferritin. (6/232)
The interactions of horse spleen ferritin and its derivative apoferritin with H+ ions were studied by potentiometric and spectrophotometric titration; to aid in data analysis, heats of ionization over a limited pH range and amide content were also determined. Per apoferritin subunit, all tyrosine and cysteine side chains, two of the nine lysine side chains and at least three of the six histidine side chains were found not to titrate; a preliminary but self-consistent analysis of the titration data is proposed. The titration curve of ferritin was identical with that of apoferritin in the pH range 5.5 to 3. In addition, under the conditions used, the reactivities of ferritin histidines to bromoacetate and of ferritin lysines to formaldehyde were identical with those in apoferritin. Above pH 8, a time-dependent titration of the ferritin core occurs which prevents comparison of the titration curves of the two proteins in this region. However, in the pH regions 5.5 to 7.5, two extra groups per subunit titrate reversibly in ferritin relative to apoferritin. Moreover, although the isoionic points of ferritin and apoferritin are identical in water, the isoionic point of ferritin is 0.5 pH unit lower than that of apoferritin in 0.16 to 1 M KCl. The different effects of KCl and NaCl on the two proteins indicate the presence of cation binding sites in ferritin that are absent in apoferritin and possibly also the presence of anion binding sites in apoferritin that are occupied in ferritin by anions of the core. The difference between the isoionic points of the two proteins in KCl has been interpreted to indicate the presence of approximately 2 phosphate residues per ferritin subunit which serve as cation binding sites and which are negatively charged at the isoionic point in KCl. These phosphates may also represent the additional residues that titrate in ferritin between pH 5.5 and 7.5, or may interact with positively charged residues on the inner surface of the ferritin shell, or both. (+info)Expression and characterization of the chemokine receptors CCR2 and CCR5 in mice. (7/232)
The chemokine receptors CCR2 and CCR5 play important roles in the recruitment of monocytes/macrophages and T cells. To better understand the role of both receptors in murine models of inflammatory diseases and to recognize potential problems when correlating these data to humans, we have generated mAbs against murine CCR2 and CCR5. In mice CCR2 is homogeneously expressed on monocytes and on 2--15% of T cells, closely resembling the expression pattern in humans. In contrast to humans, murine NK cells are highly CCR5 positive. In addition, CCR5 is expressed on 3--10% of CD4 and 10--40% of CD8-positive T cells and is weakly detectable on monocytes. Using a model of immune complex nephritis, we examined the effects of inflammation on chemokine receptor expression and found a 10-fold enrichment of CCR5(+) and CCR2(+) T cells in the inflamed kidneys. The activity of various chemokines and the antagonistic properties of the mAbs were measured by ligand-induced internalization of CCR2 and CCR5 on primary leukocytes. The Ab MC-21 (anti-CCR2) reduced the activity of murine monocyte chemotactic protein 1 by 95%, whereas the Ab MC-68 (anti-CCR5) blocked over 99% of the macrophage-inflammatory protein 1alpha and RANTES activity. MC-21 and MC-68 efficiently blocked the ligand binding to CCR2 and CCR5 with an IC(50) of 0.09 and 0.6--1.0 microg/ml, respectively. In good correlation to these in vitro data, MC-21 almost completely prevented the influx of monocytes in thioglycollate-induced peritonitis. Therefore, both Abs appear as useful reagents to further study the role of CCR2 and CCR5 in murine disease models. (+info)Chemokine and chemokine receptor expression during initiation and resolution of immune complex glomerulonephritis. (8/232)
Chemokines participate in leukocyte infiltration, which plays a major role in glomerular injury during immune complex glomerulonephritis (IC-GN). Because target cell expression of chemokine receptors (CCR) is thought to mediate leukocyte migration, the expression pattern of chemokines and CCR in a model of IC-GN was examined. The transient course and predominant glomerular pathology of this model allows the examination of both the induction and resolution phases of IC-GN. GN was induced in mice by daily apoferritin injection for 2 wk. Urine samples and kidneys were obtained at 1, 2, and 4 wk. Albuminuria was noted at 2 wk, but resolved after 4 wk. This was associated with glomerular IC deposits and mesangial proliferation. Glomerular macrophage infiltration was prominent at 1 and 2 wk, which resolved at 4 wk. Expression of monocyte chemoattractant protein-1 (MCP-1) and RANTES mRNA was upregulated at week 1 and decreased to control levels at weeks 2 and 4. The expression was localized to glomeruli by in situ hybridization and immunohistochemistry. The mRNA of CCR1, CCR2, and CCR5 but not CCR3 or CCR4 were upregulated at week 1 and decreased at weeks 2 and 4. Expression of CCR5 was located to the glomerulus by in situ hybridization and quantitative reverse transcription-PCR of isolated glomeruli. In summary, in a model of transient IC-GN, MCP-1 and RANTES and their receptors CCR1, CCR2, and CCR5 are expressed early and are already downregulated at the peak of proteinuria and leukocyte infiltration. Resolution of glomerulonephritis is associated with a return to baseline of chemokine and CCR expression. Therefore, it is concluded that glomerular MCP-1 and RANTES production directs circulating leukocytes that express CCR1, CCR2, and CCR5 into the glomerulus. After initiating GN, MCP-1 and RANTES and their receptors are readily downregulated. (+info)
Mouse Ferritin light chain 1, Ftl1 ELISA KIT
FTH1 - Wikipedia
Correction: Ferritin heavy chain protects the developing wing from reactive oxygen species and ferroptosis | proLékaře.cz
4v6b - Proteopedia, life in 3D
Molecular Vision: Distribution of ferritin chains in canine
lenses with and without age-related nuclear cataracts
Ferritin Heavy Chain in Triple Negative Breast Cancer: A Favorable Prognostic Marker that Relates to a Cluster of...
The Amino-acid-sequence of Human Spleen Apoferritin
| DIAL.pr - BOREAL
FTLP17 Gene - GeneCards | FTLP17 Pseudogene
ELISA Kit for Ferritin, Heavy Polypeptide (FTH) | SED021Mu | Mus musculus (Mouse) CLOUD-CLONE CORP.(CCC)
Bibliography | NNTC
Structure Cluster
- 1DAT: CUBIC CRYSTAL STRUCTURE RECOMBINANT HORSE L APOFERRITIN 3D Similarity Report Page
Supplement advice please - Thyroid UK | HealthUnlocked
Guest Post: A Five Minute Example of HFT Shenanigans | Zero Hedge
FTL: Faster Than Light on the App Store
Recommended - Similar items - FTL: Faster Than Light + Soundtrack
FTL: The Fatal Frontier, Sector 7 | Rock, Paper, Shotgun
The new Liquiphant FTL62 - digital, simple and safe | Endress+Hauser
Vibronic - Liquiphant FTL81 point level switch | Endress+Hauser
2FTL | Genus
FTL258 - Dare to Dream | Stampers With Altitude!!!
Northside Manipal Hospital in Malleshwaram, Bangalore - Book Appointment, View Contact Number, Feedbacks, Address | Dr....
Hereditary Hyperferritinemia Cataract Syndrome - EyeWiki
Hereditary Hyperferritinemia Cataract Syndrome - EyeWiki
Homozygous mutation of the 5UTR region of the L-Ferritin gene in the hereditary hyperferritinemia cataract syndrome and its...
Ferritin | Clinical Diagnostics | Bio-Rad
Cytoprotective effects of ferritin on doxorubicin-induced breast cancer cell death. - hepaton
Cadmium in the structure of Cubic Crystal Structure of Native Horse Spleen Ferritin (pdb 1ier)
Incorporation and release of inorganic phosphate in horse spleen ferritin | Biochemical Journal
Ferritin and ferritin isoforms I : structure-function relationships synthesis degradation and secretion
Goat Anti Human Ferritin Antibody - Serum | Bio-Rad
Main Page
Chemistry 333: Protein Structure and Function
The response of intracellular and surface ferritin after T-cell stimulation in vitro | Clinical Science | Portland Press
Homeostatic Mechanisms for Iron Storage Revealed by Genetic Manipulations and Live Imaging of Drosophila Ferritin | Genetics
CPC #13: HYPERFERRITIN CATARACT SYNDROME - Living an Optimized Life
FTL monoclonal antibody, clone 1E7 - (H00002512-M04) - Products - Abnova
FTL monoclonal antibody, clone 3E12 - (H00002512-M09) - Products - Abnova
Pets Megastore : **HFT Ezybones Medium 100s - US$225.52 [AU$281.70] discount medical supplies for pets
Humanized archaeal ferritin as a tool for cell targeted delivery - Nanoscale (RSC Publishing)
FTL: Faster Than Light for Windows (2012) - MobyGames
Hft | Get involved | DIY Fundraising - support and guidance
Pets Megastore : HFT Ezybones Sml Dogs 6 Pack - US$18.33 [AU$22.90] discount medical supplies for pets
Archive for March 2021
FABORY HHCS,7/8-9x5-1/2,Black Oxide,Gr 5,PK20 - 42HA89|B01000.087.0550 - Grainger
FABORY HHCS,3/8-24x2,Black Oxide,Gr 8,PK300 - 42HP52|B04160.037.0200 - Grainger
HAProxy vs nginx: Why you should NEVER use nginx for load balancing! - The HFT Guy
HAProxy vs nginx: Why you should NEVER use nginx for load balancing! - The HFT Guy
Posts - Bank of Canada
Gentaur Molecular :BioBasic \ Ferritin \ FB0457
Gentaur Molecular :BioBasic \ Ferritin \ FB0457
FTH1
Addison JM, Fitton JE, Lewis WG, May K, Harrison PM (November 1983). "The amino acid sequence of human liver apoferritin". FEBS ... "Cloning and sequencing of a full length cDNA coding for a human apoferritin H chain: evidence for a multigene family". The EMBO ... "Structure of gene and pseudogenes of human apoferritin H". Nucleic Acids Research. 14 (2): 721-36. doi:10.1093/nar/14.2.721. ...
Ferritin light chain
Addison JM, Fitton JE, Lewis WG, May K, Harrison PM (November 1983). "The amino acid sequence of human liver apoferritin". FEBS ... A study conducted with different apoferritins with distinct compositions of heavy and light subunits revealed that both ... "Cloning of the gene coding for human L apoferritin". Nucleic Acids Res. 14 (7): 2863-76. doi:10.1093/nar/14.7.2863. PMC 339708 ... "Negative and positive elements in the promoter region of the human apoferritin L gene". Biochem. Biophys. Res. Commun. 215 (1 ...
Philip Bourne
"Helix packing and subunit conformation in horse spleen apoferritin". Nature. 288 (5788): 298-300. Bibcode:1980Natur.288..298C. ...
Ferritin
Apoferritin binds to free ferrous iron and stores it in the ferric state. As ferritin accumulates within cells of the ... Ferritin that is not combined with iron is called apoferritin.[citation needed] Ferritin genes are highly conserved between ... "Ferritin - Homo sapiens (Human)". Look up ferritin or apoferritin in Wiktionary, the free dictionary. Ferritins at the US ... "Size-Selective Olefin Hydrogenation by a Pd Nanocluster Provided in an Apo-Ferritin Cage". Angewandte Chemie. 43 (19): 2527-30 ...
Neural binding
Ho, Ru-Hung; Chen, Yu-Hung; Wang, Chong Mou (June 2012). "Surface differentiation of ferritin and apoferritin with atomic force ...
Polysaccharide-protein conjugate
Luo, Yanan; Wang, Xuenv; Du, Dan; Lin, Yuehe (2015-09-15). "Hyaluronic acid-conjugated apoferritin nanocages for lung cancer ...
Sam Granick
A second paper, by Granick and Michaelis, showed that iron could be removed from ferritin to produce apoferritin. Granick went ... Kresge, Nicole; Robert D. Simoni; Robert L. Hill (2004-12-03). "The Characterization of Ferritin and Apoferritin by Leonor ...
Biomimetics
This is an alternative to the apoferritin cage technique currently used to synthesize uniform CdSe nanoparticles. Such ... Apoferritin". Chemistry Letters. 33 (9): 1158-1159. doi:10.1246/cl.2004.1158. Benyus, J. M. (2001). Along Came a Spider. Sierra ...
Sjors Scheres
In 2020, Scheres and collaborators used RELION to reach atomic resolution for a cryo-EM reconstruction of apo-Ferritin. Besides ...
List of MeSH codes (D12.776)
... apoferritin MeSH D12.776.556.579.374.187 - hemerythrin MeSH D12.776.556.579.374.281 - inositol oxygenase MeSH D12.776.556.579. ...
Iron in biology
... which is accomplished by Fe2+ binding to apoferritin (in which case the iron will leave the body when the cell dies and is ...
List of MeSH codes (D12.776.157)
... apoferritin MeSH D12.776.157.427.374.187 - hemerythrin MeSH D12.776.157.427.374.375 - iron-sulfur proteins MeSH D12.776.157.427 ...
Human iron metabolism
... which is accomplished by Fe2+ binding to apoferritin (in which case the iron will leave the body when the cell dies and is ...
Pauline Harrison
Harrison, Pauline M. (1959), "The structures of ferritin and apoferritin: Some preliminary X-ray data", Journal of Molecular ...
apoferritin - Atlas of Science
FTL gene: MedlinePlus Genetics
apoferritin
Posted in Achievements, Data, Papers , Tagged apoferritin, cryo-EM, data, Glacios , Leave a reply Vitrified apoferritin in ... Tag Archives: apoferritin. Our paper on the 2.7 Å structure of apoferritin with the Glacios and the Falcon 3EC is available as ... Posted in Data , Tagged apoferritin, data, Glacios , Leave a reply First cryo-image from our JEOL. Posted on 15/01/2019. by ... Posted in Data , Tagged apoferritin, cryo-EM, Glacios, imaging, Warp , Leave a reply a sneak peek into Halle automated ...
apo-ferritin - KPWu's group research site
Advanced Search Results - Public Health Image Library(PHIL)
NIOSHTIC-2 Search Results - Full View
Different apoferritin templated metal phosphates were used as distinguishable signal reporters (apoferritin templated cadmium ... Simultaneous immunoassay of phosphorylated proteins based on apoferritin templated metallic phosphates as voltammetrically ... Apoferritin templated metallic phosphates; Phoaphorylated protein; Electrochemical detection ... phosphates (ATCP) and apoferritin templated lead phosphates (ATLP)) to enhance the detection sensitivity. Here, magnetic Fe3O4 ...
FTH1 - Wikipedia
Addison JM, Fitton JE, Lewis WG, May K, Harrison PM (November 1983). "The amino acid sequence of human liver apoferritin". FEBS ... "Cloning and sequencing of a full length cDNA coding for a human apoferritin H chain: evidence for a multigene family". The EMBO ... "Structure of gene and pseudogenes of human apoferritin H". Nucleic Acids Research. 14 (2): 721-36. doi:10.1093/nar/14.2.721. ...
publication list | Max-Planck-Institut für Polymerforschung
Ferritin - a multifaceted protein scaffold for biotherapeutics | Experimental & Molecular Medicine
An apoferritin-based drug delivery system for the tyrosine kinase inhibitor gefitinib. Adv. Healthc. Mater. 4, 2816-2821 (2015 ... Szabo, I., Crich, S. G., Alberti, D., Kalman, F. K. & Aime, S. Mn loaded apoferritin as an MRI sensor of melanin formation in ... Li, M., Wu, D., Chen, Y., Shan, G. & Liu, Y. Apoferritin nanocages with Au nanoshell coating as drug carrier for multistimuli- ... A self-targeting, dual ROS/pH-responsive apoferritin nanocage for spatiotemporally controlled drug delivery to breast cancer. ...
Transferrin and H-ferritin involvement in brain iron acquisition during postnatal development: impact of sex and genotype<...
CRYO ARM™ series microscopes for cryo-EM in structural analysis of proteins and viruses | | JEOL Resources
5: Results from apo-ferritin sample in an AG-compatible cartridge.. Throughput challenge I. Figure 6 shows results from an ... 6: Results from apo-ferritin obtained to determine the amount of data/time needed to break 1.5Å. The results were obtained ... Figure 5 shows results obtained from apo-ferritin using an AG-compatible cartridge after only 30 minutes of data collection. ... 7: Results from apo-ferritin sample. Final map is at 1.29Å resolution. ...
Protein
Related articles
Profile for Richard Strange at the University of Essex
Biomimetics - greenergyexpo.eu
IUCr) Acta Crystallographica Section D Volume 78, Part 1, January 2022
EMDB references: apoferritin in 1.7% glycerol acquired at 200 keV, EMD-24795; apoferritin in 20% glycerol acquired at 200 keV, ... EMD-24796; apoferritin in 20% glycerol acquired at 300 keV, EMD-24797; aldolase in 20% glycerol acquired at 200 keV, EMD-24798 ... Å resolution reconstruction of mouse apoferritin (∼500 kDa) and an ∼3.3 Å resolution reconstruction of rabbit muscle aldolase ...
Browse by Subjects - Nottingham ePrints
Direct visualization of polypeptide shell of ferritin molecule by atomic force microscopy<...
These structures observed in the AFM images are quite similar to the electron density map near the top of the apoferritin ... These structures observed in the AFM images are quite similar to the electron density map near the top of the apoferritin ... These structures observed in the AFM images are quite similar to the electron density map near the top of the apoferritin ... These structures observed in the AFM images are quite similar to the electron density map near the top of the apoferritin ...
Role of ferritin and ferroportin genes in unexplained hyperferritinaemia<...
PDF] Facile fabrication of suspended as-grown carbon nanotube devices | Semantic Scholar
Troponin T, Human, Recombinant (Rec. TnT) - scrippslabs.com
This preparation of Recombinant Troponin T (Rec. TnT) is produced in E. coli and is provided as a powder, lyophilized from 10 mM HCl. Resuspend in 25 mM Tris, 7 M Urea, 15 mM b-Mercaptoethanol, 5 mM EDTA, pH 7.5. Purity is ≥95% by SDS-PAGE. Protein content is determined by A280 prior to lyophilization. (Emg/ml = 0.48). ...
Determinação da estrutura secundária de molécula única de proteínas através de nanoespectroscopia de absorção infravermelha |...
Shake-it-off: A simple ultrasonic cryo-EM specimen preparation device - preLights
As a proof-of-principle the authors obtain the structure of horse spleen apoferritin at 2.6 Å resolution, which is on par with ... Apoferritin is an extremely stable protein sample, has this set-up been tested with air-sensitive samples? ... Apoferritin is an extremely stable protein sample, has this set-up been tested with air-sensitive samples? ...
Search results - ORA - Oxford University Research Archive
H ferritin gene silencing in a human metastatic melanoma cell line : A proteomic analysis
Kwang Meyung Kim - Research output - Korea University
Lai, M. H., Lee, S., Kim, K. M. & Kong, H., 2014 Jan 1, Nanoscale Science and Engineering Forum 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future. AIChE, 1 p. (Nanoscale Science and Engineering Forum 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future).. Research output: Chapter in Book/Report/Conference proceeding › Conference contribution ...
Gregory M Vercellotti - Research output
- [email protected]
Vinchi, F., Sparla, R., Passos, S. T., Sharma, R., Vance, S. Z., Zreid, H. S., Juaidi, H., Manwani, D., Yazdanbakhsh, K., Nandi, V., Silva, A. M. N., Agarvas, A. R., Fibach, E., Belcher, J. D., Vercellotti, G. M., Ghoti, H. & Muckenthaler, M. U., May 2021, In: British journal of haematology. 193, 3, p. 637-658 22 p.. Research output: Contribution to journal › Article › peer-review ...
Refeyn | Measuring molecules with light | Applications
Advances in Size Exclusion Chromatography for the …...Advances in Size Exclusion Chromatography for the Analysis of...
IgM Pentamer (900 Kda), 2. Thyroglobulin (667 KDa), 3. Apoferritin (443KDa), 4. β-Amylase (200 Kda), 5. IgG (150 KDa), 6. BSA ( ... IgM Pentamer (900 Kda), 2. Thyroglobulin (667 KDa), 3. Apoferritin (443KDa), 4. β-Amylase (200 KDa), 5. IgG (150 KDa). Sample ... half-height measurement) calculated for the separation between the apoferritin monomer (MW=443 KDa) and ... for the separation of apoferritin (443 KDa). Sample injection volumes and flow rate were normalized for column geometry. UV ...
Protein5
- As examples to demonstrate the capabilities of this technique, single molecules of two different protein species, apoferritin and thyroglobulin, were successfully measured, and their secondary structures were accurately determined from the high-quality AFM-IR spectra. (bruker.com)
- Apoferritin is an extremely stable protein sample, has this set-up been tested with air-sensitive samples? (biologists.com)
- Apoferritin (ApoF) is a globular protein complex consisting of 24 protein subunits forming a nanocage. (refeyn.com)
- The protein components of a number of complexes, such as enzymes (APOENZYMES), ferritin (APOFERRITINS), or lipoproteins (APOLIPOPROTEINS). (lookformedical.com)
- Researchers from the Medical Research Council Laboratory of Molecular Biology in Cambridge, UK, obtained a 1.2 ångström resolution structure of the iron-storing protein apoferritin. (rdworldonline.com)
Ferritin3
- The difference between the tightly peaked apoferritin mass distribution and the broad mass distribution of ferritin with an iron core, can be seen in Figure 2. (refeyn.com)
- The ferritin that is not combined with iron is known as apoferritin. (differencebetween.com)
- Macara IG, Hoy TG, Harrison PM. The formation of ferritin from apoferritin. (wikipathways.org)
Nanocage2
- They used apoferritins, which are proteins with hollow nanocage structures, to encapsulate tiny nanocatalyst particles. (asiaresearchnews.com)
- Elzenaty O, Luciani P , Aleandri S *. A lipidic mesophase with tunable release properties for the local delivery of macromolecules: the apoferritin nanocage, a case study. (lucianiresearch.com)
Proteins1
- Simultaneous immunoassay of phosphorylated proteins based on apoferritin templated metallic phosphates as voltammetrically distinguishable signal reporters. (cdc.gov)
Sensitivity1
- Different apoferritin templated metal phosphates were used as distinguishable signal reporters (apoferritin templated cadmium phosphates (ATCP) and apoferritin templated lead phosphates (ATLP)) to enhance the detection sensitivity. (cdc.gov)
Successfully1
- We have successfully vitrified apoferritin in own Vitrobot Mark 4. (uni-halle.de)
Structure7
- Our paper on the 2.7 Å structure of apoferritin with the Glacios and the Falcon 3EC is available as a preprint! (uni-halle.de)
- In this work, we have determined the atomic structure of mouse apoferritin using the Glacios microscope. (uni-halle.de)
- Structure of gene and pseudogenes of human apoferritin H". Nucleic Acids Research. (wikipedia.org)
- These structures observed in the AFM images are quite similar to the electron density map near the top of the apoferritin viewed down from a 4-fold axis structure reported previously (S. H. Banyard, D. K. Stammers, and P. M. Harrison, 1978. (elsevier.com)
- As a proof-of-principle the authors obtain the structure of horse spleen apoferritin at 2.6 Å resolution, which is on par with previous articles. (biologists.com)
- We have determined the first true-atomic resolution structure of apoferritin by cryo-EM in which individual atoms are clearly resolved. (asso.fr)
- 1.42 Angstrom Apoferritin structure determined using G1 Titan krios S-FEG operated at 300kV, zero loss imaging using Gatan BioQuantum energy filter operated at 10eV slit width and imaged using K2 camera. (pdbj.org)
Gene1
- A ny gene located in or near the level of anxiety, tension, and copious irrigation is performed to identify the tubes failure to fill in the gi tract but still present some amount of apoferritin synthesised is reduced if the drug of choice for diagnosing urinary tract infection in primary care. (windmillharbourmarina.org)
Data1
- Thanks to Warp (http://www.warpem.com/warp/), an amazing on-the-fly image processing suite for cryo-EM data, we are able not only to acquire beautiful apoferritin images with EPU, but also estimate the quality of our image acquisition on the fly! (uni-halle.de)
Protein apoferritin1
- Ferritin - A water-soluble complex of iron hydroxide with the protein apoferritin, found in liver, spleen, bone marrow cells, reticulocytes and small amounts of blood serum, where it performs the function of transporting iron. (synevo.ge)
Ferroxidase activity1
- The heavy chain in the apoferritin molecule exerts ferroxidase activity, catalyzing the oxidation of Fe 2+ into Fe 3+ , which prevents iron-induced cyclic red-ox reactions that would spread and amplify the oxidative damage. (medscape.com)
Molecule1
- When needed, the iron molecule is released from the apoferritin shell and bind to transferrin, whic. (ceetexams.co.in)
Crystals3
- Here we used tungsten and iron deposition to form doped iron oxide crystals inside the apoferritin cavity to form a WFe nanoparticle with a disordered crystal and un-coupled atomic magnetic moments. (wustl.edu)
- Here, apoferritin single crystals are used as templates for the production of mesoporous silica. (aalto.fi)
- Successive data sets were collected from four holoferritin and three apoferritin crystals. (ox.ac.uk)
Molecular2
- The WFe apoferritin nanoparticles were not cytotoxic up to 700 nm particle concentrations, making them potentially important for targeted molecular MRI. (wustl.edu)
- His values of the molecular weights of apoferritin from man, horse, and dog agreed with each other within a range of possible error of little more than 1 per cent. (nih.gov)
Structures3
- Apoferritins are shell-like structures containing nanocavities and ferroxidase activities. (nih.gov)
- As examples to demonstrate the capabilities of this technique, single molecules of two different protein species, apoferritin and thyroglobulin, were successfully measured, and their secondary structures were accurately determined from the high-quality AFM-IR spectra. (bruker.com)
- Apoferritin can be removed by calcination to yield solid state structures with the crystalline periodicity retained in the mesopores. (aalto.fi)
Iron2
- Synthesis of apoferritin is induced at both the transcriptional and posttranscriptional levels by the presence of free iron. (medscape.com)
- Wishing to check his own method on a stable, reproducible protein, he chose apoferritin (the iron-free fraction of heme discovered by Granick). (nih.gov)
Single1
- We demonstrated this by using single-particle cryo-EM and cryo-electron tomography (cryo-ET) to examine hemagglutinin, insulin receptor complex, and apoferritin. (nih.gov)